Consulta: subjectFacets:"Transgenic"
Registros recuperados: 27
Data/hora: 15/06/2024 16:18:39

Attitudes towards genomic: Research in four Latin American countries
Provedor de dados: 69
Autores: Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando.
The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,...
Tipo: Journal article
Palavras-chave: Cloning; Genome; Social representations; Transgenic.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003

A practical approach to the understanding and teaching of RNA silencing in plants
Provedor de dados: 69
Autores: Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián.
Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach...
Tipo: Journal article
Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002

Chitinase activities, scab resistance, mycorrhization rates and biomass of own-rooted and grafted transgenic apple
Provedor de dados: 74
Autores: Schäfer,Tina; Hanke,Magda-Viola; Flachowsky,Henryk; König,Stephan; Peil,Andreas; Kaldorf,Michael; Polle,Andrea; Buscot,François.
This study investigated the impact of constitutively expressed Trichoderma atroviride genes encoding exochitinase nag70 or endochitinase ech42 in transgenic lines of the apple cultivar Pinova on the symbiosis with arbuscular mycorrhizal fungi (AMF). We compared the exo- and endochitinase activities of leaves and roots from non-transgenic Pinova and the transgenic lines T386 and T389. Local and systemic effects were examined using own-rooted trees and trees grafted onto rootstock M9. Scab susceptibility was also assessed in own-rooted and grafted trees. AMF root colonization was assessed microscopically in the roots of apple trees cultivated in pots with artificial substrate and inoculated with the AMF Glomus intraradices and Glomus mosseae. Own-rooted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple; Chitinase; Mycorrhiza; Transgenic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300014

Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco
Provedor de dados: 74
Autores: Tizaoui,Kalthoum; Kchouk,Mohamed Elyes.
Transgene integration into plant genomes is a complex process accompanied by molecular rearrangements. Classic methods that are normally used to study transgenic population genetics are generally inadequate for assessing such integration. Two major characteristics of transgenic populations are that a transgenic genome may harbor many copies of the transgene and that molecular rearrangements can create an unstable transgenic locus. In this work, we examined the segregation of T1, T2 and T3 transgenic tobacco progenies. Since transfer DNA (T-DNA) contains the NptII selectable marker gene that confers resistance to kanamycin, we used this characteristic in developing a method to estimate the number of functional inserts integrated into the genome. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copy number variation; Gene rearrangements; Genetic inheritance; Transgenic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400015

Ensaio de competição de cultivares tolerantes ao glifosato da rede soja sul de pesquisa, safra 2009/10.
Provedor de dados: 14
Autores: BERTAGNOLLI, P. F.; COSTAMILAN, L. M.; NICOLAU, M.; VERNETTI JUNIOR, F. de J.; STECKLING, C.; ROVERSI, T.; RUBIN, S. de A. L.; GONÇALVES, J. A.; CASTRO, R. L. de; GABE, N. L.; OLIVEIRA, M. A. R. de; VICENTE, D.; MATZENBACHER, R.; GIASSON, N. F.; BAGATINI, N. P.; SILVA, A. E. L. da.
bitstream/item/128741/1/ID41911LVdocumentos102-p41-58.pdf
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Soybean; Transgenic.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/882340

Assessing the effects of genotype-by-environment interaction on epigenetic, transcriptomic, and phenotypic response in a Pacific salmon
Provedor de dados: 5
Autores: Christensen, Kris A; Le Luyer, Jeremy; Chan, Michelle T T; Rondeau, Eric B; Koop, Ben F; Bernatchez, Louis; Devlin, Robert H.
Genotype-by-environment (GxE) interactions are non-parallel reaction norms among individuals with different genotypes in response to different environmental conditions. GxE interactions are an extension of phenotypic plasticity and consequently studying such interactions improves our ability to predict effects of different environments on phenotype as well as the fitness of genetically distinct organisms and their capacity to interact with ecosystems. Growth hormone transgenic coho salmon grow much faster than non-transgenics when raised in tank environments, but show little difference in growth when reared in nature-like streams. We used this model system to evaluate potential mechanisms underlying this growth rate GxE interaction, performing RNA-seq to...
Tipo: Text
Palavras-chave: Epigenetics; Transcriptome; GxE; Transgenic; Growth hormone; Salmonid.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00677/78890/81188.pdf

Histocytological examination on organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant
Provedor de dados: 85
Autores: Guan,Z-J; Guo,B; Huo,Y-L; Dai,J-K; Wei,Y-H.
The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige &amp;amp; Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron microscopy indicated that there were significant changes in morphology and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histocytological; Organogenesis; Somatic embryogenesis; HBsAg; Transgenic; Cherry tomato; Mutant.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100008

The role of glyphosate in RR soybean production and seed quality
Provedor de dados: 87
Autores: Albrecht,L.P.; Albrecht,A.J.P.; Braccini,A.L.; Oliveira Jr.,R.S.; Zobiole,L.H.S.; Ávila,M.R.
This study aimed to evaluate the production components and quality of RR soybean seeds (Roundup Ready®), after application of increasing rates of gliphosate. Field experiments were conducted in Mandaguari, Paraná, during two seasons. Treatments consisted of five doses of glyphosate. All applications were performed once, between development stages V4 and V5. The experiment was arranged in a completely randomized block design, with four replicates. Data were subjected to analysis of variance, and when significant, t-tests and a regression analysis were applied to verify the behavior of the treatments. The physiological and sanitary quality, yield and mass of one thousand seeds were evaluated. The results indicated that seed quality can be adversely affected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Herbicide; Transgenic.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582014000200018

Ex-Ante Evaluation of Drought Tolerant Varieties in Eastern and Central Africa
Provedor de dados: 31
Autores: Kostandini,   Genti; Mills,   Bradford F.; Mykerezi,   Elton.
Tipo: Conference Paper or Presentation
Palavras-chave: Transgenic; Drought Tolerance; Household Benefits; Consumer/Household Economics; Crop Production/Industries.
Ano: 2008
URL: http://purl.umn.edu/49372

Welfare Measures and Mandatory Regulation for Transgenic Food in the European Union: A Theoretical Framework for the Analysis
Provedor de dados: 31
Autores: Diez,   Maria Del Carmen Fernandez.
This paper presents an analytical framework for studying the impact of mandatory labelling regulation for transgenic food. We compare Genetically Modified (GM) and conventional crop markets and identify gains for food processors prior to mandatory labelling and losses after this measure for the GM market. Nevertheless, food processors could obtain gains for conventional products after market disgregation. Finally, consumers will be worse off both for conventional and GM foods unless qualities other than changes to prices are considered.
Tipo: Conference Paper or Presentation
Palavras-chave: Welfare; Mandatory labelling; Transgenic; Genetically modified organism; European Union policy; Agricultural and Food Policy; Q18; K32; D62.
Ano: 2005
URL: http://purl.umn.edu/24472

Transgenic Trees: Implementation and Outcomes of the Plant Protection Act
Provedor de dados: 31
Autores: Sedjo,   Roger A.
The responsibility for protecting U.S. agriculture from pests and diseases is assigned by the Federal Plant Pest Act (FPPA) to the Animal and Plant Health Inspection Service (APHIS) of the Department of Agriculture. The Plant Protection Act (Title 7 U.S.C. Sections 7701 et seq.) gives Aphis statutory authority over genetically modified organisms (GMO), in effect assigning to APHIS a related responsibility of determining whether a genetically altered plant, crop, or tree is likely to pose unacceptable risks to the environment. Although APHIS has considerable experience with crop plants, it has only limited experience with trees. Yet the possible benefits of applying genetic engineering to trees are substantial and include industrial wood production and...
Tipo: Working or Discussion Paper
Palavras-chave: Forestry; Biotechnology; Transgenic; Tree plantations; Timber supply; Genes; GMOs; Industrial wood; Economics; Regulations; Costs; Benefits; Conservation; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 2004
URL: http://purl.umn.edu/10629

ISSUES IN DEVELOPMENT AND ADOPTION OF GENETICALLY MODIFIED (GM) WHEATS
Provedor de dados: 31
Autores: Wilson,   William W.; Janzen,   Edward L.; Dahl,   Bruce L.; Wachenheim,   Cheryl J.
Development of genetically modified (GM) wheat varieties is proceeding; however, several critical issues remain the focus of contention.  This project summarizes the current state of knowledge on some of these critical issues for commercialization of GM wheats.  Background on the evolution of GM Wheats is presented.  Then, agronomic adoption and competitiveness of GM crops; research on GM traits in wheat; consumer acceptance of GM crops (a separate section is included on issues related to consumer acceptance of GM crops); regulatory issues and status; international trade; testing, segregation, and identity preservation; and production and marketing risks are examined.  Finally, there is a description of the likely marketing system to evolve and a...
Tipo: Working or Discussion Paper
Palavras-chave: Wheat; Genetic modification; Transgenic; Marketing; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/23497

Biotechnology and Planted Forests: Assessment of Potential and Possibilities
Provedor de dados: 31
Autores: Sedjo,   Roger A.
This paper addresses the potential impact of the introduction and development of biotechnology on planted forests. It includes a description of some recent innovations in forestry including the use of traditional breeding, and also more recent innovations involving biotechnology, including the development of clonal propagation and the use of modern molecular biology techniques. In addition to describing these innovations, the paper undertakes an assessment of their probable impact on future production of the forest industry, on the global timber supply, and on future markets for timber and wood products. The paper offers a description of recent innovations in tree breeding and biotechnology, including a discussion of innovations in agriculture that have...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Plantation forests; Genetic modification; Genetic research; Economic benefits; Transgenic; GMO; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 1999
URL: http://purl.umn.edu/10862

Valuing Transgenic Cotton Technologies Using a Risk/Return Framework
Provedor de dados: 31
Autores: Bryant,   Kelly J.; Reeves,   Jeanne M.; Nichols,   Robert L.; Greene,   Jeremy K.; Tingle,   Christopher H.; Studebaker,   Glenn E.; Bourland,   Fred M.; Capps,   Charles D.; Groves,   Frank E.
Stochastic Efficiency with Respect to a Function (SERF) is used to rank transgenic cotton technology groups and place an upper and lower bound on their value. Yield and production data from replicated plot experiments are used to build cumulative distribution functions of returns for nontransgenic, Roundup Ready, Bollgard, and stacked gene cotton cultivars. Analysis of Arkansas data indicated that the stacked gene and Roundup Ready technologies would be preferred by a large number of risk neutral and risk averse producers as long as the costs of the technology and seed are below the lower bounds calculated in this manuscript.
Tipo: Journal Article
Palavras-chave: Cotton; Financial risk; Market value; SERF; Transgenic; Agribusiness; Crop Production/Industries; Risk and Uncertainty; Q12; Q16.
Ano: 2008
URL: http://purl.umn.edu/47257

Consumers Willingness to Pay to Avoid Transgenic Products
Provedor de dados: 31
Autores: Ribeiro,   Francis Lee; de Noronha,   Jose Ferreira; Noronha,   Cassia Maria Silva.
The debate on transgenic, which is embebed in asymmetrical information, has as a focal point, the risks and uncertainties to them associated. Many are the concerns and questions about the risks for the human health and the environment. Considering the interests of the productive sector in the transgenic technology, the matter sets itself in terms of how fast it wants to be settled taking into consideration the incapability of establishing certainties about the damages or benefits within the process. With the recent liberation by the Brazilian government for its production the sector starts showing segmentation. Therefore, this issue reflects in the market itself. The question is: are the consumers willing to buy transgenic? As an attempt to answer this...
Tipo: Conference Paper or Presentation
Palavras-chave: Transgenic; Contingent Valuation; Measure of Well-being; Demand and Price Analysis; Food Consumption/Nutrition/Food Safety.
Ano: 2005
URL: http://purl.umn.edu/24221

EL PAPEL DEL ADMINISTRADOR DE AGRONEGOCIOS EN LA SEGURIDAD ALIMENTARIA
Provedor de dados: 31
Autores: Guerra E.,   Guillermo A.; Aguilar Valdes,   Alfredo; Cabral Martell,   Agustin; Alvarado Martinez,   Luis Felipe; Alvarado Martinez,   Tomas Everardo; Arras Vota,   Ana Maria; Moreno Medina,   Salomon.
For decades, food security has been a worldwide problem affecting the poorest in order to find a possible solution to this dilemma that not only continues, but constantly grows with the increase in population. Agriculture, as the main food supplier, has evolved and increased its productivity, and has expanded its agricultural frontiers, since previous decades, in its attempts to solve this problem. If predominant conventional agriculture (mainly dedicated to productivity) is complemented with substantial increase of transgenic, organic, agroecological, greenhouse and hydroponic crops, it will be more feasible to avoid the loss of forest, the damage and loss of soils and at the same time diminish food insecurity. Within this context, the agribusiness...
Tipo: Journal Article
Palavras-chave: Food security; Transgenic; Organics; Agro ecological; Hydroponics; Greenhouse; Agribusiness manager.; Agribusiness.
Ano: 2011
URL: http://purl.umn.edu/99477

Chemical control of stalk regrowth in glyphosate-resistant transgenic cotton
Provedor de dados: 91
Autores: Ferreira,Alexandre C. de B.; Bogiani,Julio C.; Sofiatti,Valdinei; Silva Filho,João L. da.
ABSTRACT Cotton stalk regrowth must be controlled after harvest to ensure the phytosanitary quality and sustainability of cotton farming systems. Mechanical destruction generally does not conform to the no-tillage system, and the control of cotton stalk regrowth by herbicides is little studied in Brazil. The objective of this study was to evaluate the efficiency of herbicides to control glyphosate-resistant transgenic cotton stalk regrowth. The study was conducted in three environments of the Brazilian Cerrado region, two in Santa Helena de Goiás (SHGO), during 2013 and 2014, and one in Luís Eduardo Magalhães (LEM-BA) in 2014. In each of the three environments, the experiment was carried out in a randomized complete block with four replications. Each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gossypium hirsutum; Transgenic; Stalk regrowth; Herbicides; Environment.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662018000800530

Detection of adventitious presence of genetically modified seeds in lots of non transgenic soybean
Provedor de dados: 95
Autores: Pádua,Gilda Pizzolante de; Jesus,Adriana Madeira Santos; Fronza,Vanoli; Arantes,Neylson Eustáquio; Zito,Roberto Kazuhiko.
The difficulty on identifying, lack of segregation systems and absence of suitable standards for coexistence of non trangenic and transgenic soybean are contributing for contaminations that occur during productive system. The objective of this study was to evaluate the efficiency of two methods for detecting mixtures of seeds genetically modified (GM) into samples of non-GM soybean, in a way that seed lots can be assessed within the standards established by seed legislation. Two sizes of soybean samples (200 and 400 seeds), cv. BRSMG 810C (non-GM) and BRSMG 850GRR (GM), were assessed with four contamination levels (addition of GM seeds, for obtaining 0.0%, 0.5%, 1.0%, and 1.5% contamination), and two detection methods: immunoassay of lateral flux (ILF) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Glyphosate; Transgenic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000400007

Impact of Bt sweet corn on lepidopteran pests in Midwestern Brazil
Provedor de dados: 63
Autores: Schneider,Altair Marcos; Gontijo,Lessando Moreira; Costa,Lilian Lucia.
ABSTRACT: While the cultivation of conventional Bt corn has been widely adopted in Brazil, the production of Bt sweet corn is still incipient. The main lepidopteran pests found attacking conventional and sweet corn in Brazil are Spodoptera frugiperda (J.E. Smith) and Helicoverpa zea (Boddie). Additionally, a new harmful Lepidoptera species has recently appeared in Brazil, Helicoverpa armigera (Hübner). Since Bt sweet corn in Brazil is as yet cultivated on a limited scale there is no scientific information available about the performance of Bt sweet corn against lepidopterans out in the field. Thus, we conducted two field experiments to evaluate the efficacy of Bt sweet corn (event MON89034), alone and in conjunction with insecticides, against major...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spodoptera frugiperda; Helicoverpa; Transgenic; Insecticide.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001300214

Identification of geneticaly modified soybean seeds resistant to glyphosate
Provedor de dados: 63
Autores: Tillmann,Maria Ângela André; West,Shirlie.
Advances in genetic engineering permit the modification of plants to be tolerant to certain herbicides that are usually not selective. For practical and commercial purposes, it is important to be able to detect the presence or absence of these traits in genotypes. The objective of this research was to develop a procedure for identifying genetically modified soybean (Glycine max L. Merr.) with resistance to the herbicide glyphosate. Two studies were conducted based on germination test. In the first study, soybean seeds were pre-imbibed in paper towel with the herbicide solutions, then transferred to moist paper towel for the germination test. In the second study, seeds were placed directly in herbicide solutions in plastic cups and tested for germination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenic; Germination; Detection; Glyphosate.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000300017

Efficacy of alternative herbicides to glyphosate in the weed control ín glyphosate and sulfonyiurea-tolerant soybean
Provedor de dados: 109
Autores: Moreira Silva,André Felipe; Giraldeli,Ana Ligia; Soares da Silva,Gustavo; da Silva Araújo,Lucas; Nogueira Lima,Robson Josadaque; Góes de Oliveira,Diogo Alexandre; Paiola Albrecht,Alfredo Junior; Victoria Filho,Ricardo.
Abstract It is believed that ALS inhibitor herbicides in combination with ACCase inhibitors can be used as an alternative to glyphosate in weed control in RR2/STS soybeans. In the present study, the efficacy of ALS inhibitor herbicides, in combination with haloxyfop, is evaluated in the control of weeds for post- emergence application (V4) of RR/STS soybeans. The experiment is carried out in the 2017/18 season in Piracicaba, Sao Paulo (SP), Brazil. The cultivar BMX Garra RR2/STS is used, and the treatments were composed by the application of ALS inhibitor herbicides in association with haloxyfop. The experimental design was a randomized block with four replications. Were evaluated control and dry mass of weeds, symptoms of injury in soybean plants and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACCase inhibitors; ALS inhibitors; Glycine max; Herbicides mixture; Transgenic.
Ano: 2021
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122021000300292

Effects of high proline accumulation on chloroplast and mitochondrial ultrastructure and on osmotic adjustment in tobacco plants
Provedor de dados: 1
Autores: Borgo, Lucélia; Marur, Celso Jamil; Vieira, Luiz Gonzaga Esteves.
Proline accumulates in many organisms in response to abiotic stresses, such as drought and salinity. Reports have discussed the role of proline in relation to osmotic adjustment, even though no clear-cut evidence has yet been provided demonstrating this association as a defense mechanism against drought stress in plants. However, it has been indicated that the application of exogenous proline can damage the ultrastructure of chloroplasts and mitochondria. In this study, using transgenic tobacco plants carrying a mutant p5cs gene from Vigna aconitifolia under control of the CaMV 35S promoter, we show that the high levels of endogenous proline accumulated in leaves does not contribute to the osmotic adjustment in plants under water deficit conditions....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.01.00.00-9 p5cs; Transgenic; Water deficit Melhoramento Vegetal.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19097

A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
Provedor de dados: 42
Autores: Alves-Costa,Fernanda A.; Figueiredo,Márcio A.; Lanes,Carlos F.C.; Almeida,Daniela V.; Marins,Luis F.; Wasko,Adriane P.
The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differential display; GH; Growth hormone; Transgenic; Zebrafish.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200017

An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance
Provedor de dados: 52
Autores: Pileggi,Marcos; Pereira,Albanin Aparecida Mielniczki; Silva,Joandrei dos Santos; Pileggi,Sônia Alvim Veiga; Verma,Desh Pal S.
An efficient method for constructing transgenic lettuce cultivars by Agrobacterium tumefaciens was described by Torres et al., 1993. In the present work, an improvement of the above procedure is described and applied to transform the cultivar Grand Rapids with a mutated P5CS gene. The major modifications were concerned with turning more practical the transformation and regeneration protocols. Also we tried to improve transformation steps by increasing injured area in explants and prolonging co-cultivation with Agrobacteria (in larger concentration). A more significant selective pressure was used against non-transformed plants and bacteria. In these work we were concerned to obtain T1 and T2 seeds. The P5CS gene codes for a delta¹-pyrroline-5-carboxylate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress resistance; Cold resistance; Transgenic; Lettuce; Proline.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200013

Transgenic animal models for the functional analysis of vasoactive peptides
Provedor de dados: 56
Autores: Bader,M.
The interplay of vasoactive peptide systems is an essential determinant of blood pressure regulation in mammals. While the endothelin and the renin-angiotensin systems raise blood pressure by inducing vasoconstriction and sodium retention, the kallikrein-kinin and the natriuretic-peptide systems reduce arterial pressure by eliciting vasodilatation and natriuresis. Transgenic technology has proven to be very useful for the functional analysis of vasoactive peptide systems. As an outstanding example, transgenic rats overexpressing the mouse Ren-2 renin gene in several tissues become extremely hypertensive. Several other transgenic rat and mouse strains with genetic modifications of components of the renin-angiotensin system have been developed in the past...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Transgenic; Animal models; Knockout.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900011

Genetic transformation of coffee
Provedor de dados: 61
Autores: Ribas,Alessandra Ferreira; Pereira,Luiz Filipe Protasio; Vieira,Luiz Gonzaga E.
In the last 15 years, considerable advances were made in coffee genetic transformation. Different research groups in the world have been able to transform coffee with genes for insect resistance, decaffeinated coffee, herbicide resistance and control of fruit maturation. Although the majority of the research is still limited to laboratory and greenhouse studies, initial field tests with transformed coffee are beginning to appear in the literature. In this review we provide an update on the state of coffee genetic transformation, presenting technical aspects related to tissue culture systems, strategies for selection and transformation with particle bombardment, as well as the use of Agrobacterium tumefaciens. We also discuss the potential applications of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agrobacterium; Coffea; Biolistic; Genetic engineering; Transgenic.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100007

Advances in Apple Transformation Technology to Confer Resistance to Fungal Diseases in Apple Crops: A Chilean Perspective
Provedor de dados: 117
Autores: Polanco,Víctor; Paredes,Mario; Becerra,Viviana; Pérez,Eduardo.
Apple (Malus domestica Borkh.) is one of the most consumed fruit in the world. Genetic transformation is a key process to sustain this demand by permitting the potential enhancement of existing cultivars as well as the development of new cultivars resistant to pests, diseases, and storage problems that occur in the major production areas. This review summarizes the advances of genetic engineering applied to the development of resistant apple cultivars to fungus disease, with particular attention in the generation of apples resistant to Venturia inaequalis (Cooke) G.Winter, the main phytosanitary problem that affects apple crops in Chile.
Tipo: Journal article
Palavras-chave: Apple; Fungal disease; Transgenic; Cisgenesis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200013