Consulta: subjectFacets:"Transmission electron microscopy"
Registros recuperados: 32
Data/hora: 07/06/2024 09:36:16

A study of autophagy in hemocytes of the Pacific oyster,   Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...
Tipo: Text
Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf

Characterization of the protozoan parasite Marteilia refringens infecting the dwarf oyster Ostrea stentina in Tunisia
Provedor de dados: 5
Autores: Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle.
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010...
Tipo: Text
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf

Virus-like particles associated with brown muscle disease in Manila clam, Ruditapes philippinarum, in Arcachon Bay (France)
Provedor de dados: 5
Autores: Dang, C.; Gonzalez, P.; Mesmer-dudons, N.; Bonami, J-r; Caill-milly, Nathalie; De Montaudouin, X.
Recently, Manila clam, Ruditapes philippinarum, populations have suffered mortalities in Arcachon Bay (SW France). Mortality was associated with extensive lesions of the posterior adductor muscle, which become progressively brown and calcified. Ultrastructural observations by transmission electron microscopy revealed tissue degradation with necrotized muscle fibres and granulocytomas. Unenveloped virus-like particles (VLPs) were detected in muscle, granulocytic, epithelial and rectal cells. VLPs were abundant in the extracellular space, in the cytoplasm (free or enclosed in vesicles) and in the nucleoplasm of granulocytes. Nuclei and mitochondria of granulocytes displayed changes which suggested reactive oxygen species production and apoptosis induction....
Tipo: Text
Palavras-chave: Adductor muscle; Apoptosis; Brown muscle disease; Necrosis; Ruditapes philippinarum; Transmission electron microscopy.
Ano: 2009
URL: https://archimer.ifremer.fr/doc/00000/11130/7991.pdf

Detection of a mollicute-like organism in kuruma shrimp, Penaeus japonicus
Provedor de dados: 5
Autores: Choi, Dong-lim; Park, Myoung-ae; Sohn, Sang-gyu; Heo, Moon-soo; Renault, Tristan.
A filamentous form of mollicute-like bacterium was detected during a routine health survey of wild adult kuruma shrimp Penaeus japonicus. The kuruma shrimp were native to Japan and were imported to Korea. The histology showed no pathological changes. Transmission electron microscopy revealed extensive infections of hepatopancreatic epithelial cells by a pleomorphic, filamentous intracellular bacterium. The filamentous bacterium was of about 60 nm in diameter and 300 nm to more than 1µm in length. The morphology of bacteria were filamentous and branched with terminal blebs, or knobs, on the branches. They lacked the cell wall, and were bounded by the plasma membrane. They contained typical prokaryotic ribosomes and fibrillar DNA-like strands. No additional...
Tipo: Text
Palavras-chave: Transmission electron microscopy; Hepatopancreas; Mollicute; Intracellular bacteria; Penaeus japonicus.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/1996/publication-2901.pdf

Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods
Provedor de dados: 5
Autores: Takahashi, Eri; Ledauphin, Jerome; Goux, Didier; Orvain, Francis.
There is no universal method that can be applied to extract bound extracellular polymeric substances (EPS) from benthic diatoms of intertidal sediments without causing cell lysis. Six extraction methods were tested on a diatom culture of Navicula jeffreyi to establish the best compromise between high yields of carbohydrate extraction and minimum cell lysis. Extraction with distilled water provoked cell lysis (as already known). The five other extraction methods (dowex resin, artificial seawater of half salinity and extractions after pretreatment with gluteraldehyde by three methods: water, dowex water and dowex buffer) did not provoke cell lysis as shown by transmission electronic microscopy. This result was confirmed by the minimum release of internal...
Tipo: Text
Palavras-chave: Transmission electron microscopy; Protein; Navicula jeffreyi; Intertidal sediments; Immuno staining; Immunostaining; GCMS analysis; Dowex; Cell lysis; Biofilm.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7418.pdf

Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation?
Provedor de dados: 5
Autores: Coutard, Francois; Crassous, Philippe; Droguet, Michael; Gobin, Eric; Colwell, Rita; Pommepuy, Monique; Hervio Heath, Dominique.
The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population...
Tipo: Text
Palavras-chave: Transmission electron microscopy; Scanning electron microscopy; Resuscitation; Regrowth; Vibrio parahaemolyticus; VBNC state.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3117.pdf

Herpesvirus infection in European flat oysters Ostrea edulis obtained from brood stocks of various geographic origins and grown in Galicia (NW Spain)
Provedor de dados: 5
Autores: Da Silva, P; Renault, Tristan; Fuentes, J; Villalba, A.
We evaluated differences in productive traits and disease susceptibility among Ostrea edulis stocks. We produced 4 to 5 families from each of 4 oyster populations (Irish, Greek and 2 Galician) in a hatchery. Spat corresponding to 19 different families were transferred to a raft in the Ria de Arousa (Galicia, Spain) for grow-out. Samples of each family were histologically processed every month for 2 yr. One of the pathological conditions disclosed by histological examination was characterised by the occurrence of numerous abnormal cells throughout the connective tissue of various organs, showing hypertrophied nuclei with marginated chromatin and a characteristic large intranuclear acidophilic inclusion. Ultrastructural examination showed that the abnormal...
Tipo: Text
Palavras-chave: In situ hybridisation; Transmission electron microscopy; Histology; Bonamiosis; Ostreid herspesvirus 1; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3735.pdf

Transmission Electron Microscopy Study of the Neonatal Rat Tongue Mucosa Treated with Special Attention to the Bacteriae on the Epithelial Cell Membrane
Provedor de dados: 77
Autores: Picoli,Lara Cristina; Lopes,Ruberval A; Semprini,Marisa; Sala,Miguel Angel; Ogawa,Koichi; Watanabe,Ii-sei.
The epithelial cell surfaces of neonatal Wistar rat tongue mucosa were examined by transmission electron microscopic method. The tissues were fixed by modified Karnovksy solution and embedded in Spurr resin. Thin sections of 90 nm thickness were cut and examined in the Jeol 1010 transmission electron microscopy. It was revealed that the queratinized squamous epithelial cells of the tongue mucosa exposed to cadmium presented groupings of numerous bacteriae which are attached to the flat cell membrane surfaces. The coccus and bacillus are attached to the cell membrane in organized rows or at random, as clearly seen in the TEM images. At high magnification, the transmission electron microscopic images showed that bacteriae are attached by a fibrillar...
Tipo: Journal article
Palavras-chave: Epithelial cell; Tongue mucosa; Bacteria; Cadmium; Transmission electron microscopy.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300006

Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy
Provedor de dados: 77
Autores: Catroxo,M. H. B; Martins,A. M. C. R. P. F; Petrella,S; Curi,N. A; Melo,N.A.
The pigeon feces are vehicle of diseases both for humans and other animal species. In these birds, the most important viral diseases of the digestive tract are transmitted by the paramyxovirus, adenovirus and coronavirus. Avian paramyxoviruses have been isolated from a variety of species of free living and domestic birds worldwide, with several symptoms and clinical signs and economic losses. Paramyxoviruses belong to the Paramyxoviridae family and Avulovirus genus that includes nine serotypes (APMV 1 to 9). Avian adenoviruses belong to the Adenoviridae family and Aviadenovirus genus. In pigeons, cause classical adenovirosis and necrotizing hepatitis. The respiratory and enteric tracts are common targets of coronavirus. They belong to the Coronaviridae...
Tipo: Journal article
Palavras-chave: Paramyxovirus; Adenovirus; Coronavirus; Pigeons; Transmission electron microscopy.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200055

Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil
Provedor de dados: 77
Autores: Catroxo,M. H. B; Bersano,J. G; Martins,A. M. C. P. F; Petrella,S; Portugal,M. A. S. C; Souza,O. S.
The myxomatosis is a contagious worldwide disease caused by poxvirus which infects domestic and wild rabbits. In the present study we present two distinct outbreaks of myxomatosis when raising rabbits, one for commercial purpose of production of meat and skins and, another one for the commercialization of ornamental rabbits. The observed signs were ocular, auricular, nasal, testis lesions and many times scattered throughout the body of the animals. The lesions were characterized by formation of nodules that by palpation disclosed gummy or gelatinous aspect. At the transmission electron microscopy, all the skin and crust samples were analyzed by negative staining technique. A great number of particles with morphology similar to the poxvirus, some enveloped...
Tipo: Journal article
Palavras-chave: Myxomatosis; Poxvirus; Rabbits; Transmission electron microscopy.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200039

Detection of Coronavirus in Capybaras (Hydrochoeris hydrochaeris) by Transmission Electron Microscopy in São Paulo, Brazil
Provedor de dados: 77
Autores: Catroxo,M. H. B; Miranda,L.B; Lavorenti,A; Petrella,S; Melo,N. A; Martins,A. M. C. P. R. F.
Coronaviruses are enveloped, positive-stranded RNA, belongs to the Nidovirales order, Coronaviridae family and are classified into three groups. Groups 3a and 3c comprising the avian coronaviruses. They are worldwide in distribution and can cause respiratory, enteric and in some cases hepatitis and neurological diseases in a wide variety of animals, resulting in economic losses to breeders and breeding. In this study, we describe an outbreak of diarrhea in a herd of capybaras found in the city of Piracicaba, São Paulo, Brazil. The animals had sudden symptoms of anorexia, profuse and watery diarrhea, yellowish or greenish, dehydration, weight loss, sharp and twisting of limbs leading to death in less than a week, mostly weaned and with an age of three...
Tipo: Journal article
Palavras-chave: Coronavirus; Capybaras; Transmission electron microscopy.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200035

Identification of Poxvirus Under Transmission Electron Microscopy During Outbreak Period in Wild Birds, in São Paulo, Brazil
Provedor de dados: 77
Autores: Catroxo,M. H. B; Pongiluppi,T; Melo,N. A; Milanelo,L; Petrella,S; Martins,A. M. C. P. F; Rebouças,M. M.
Avianpox is a highly contagious disease infecting both commercial and wild birds, causing great damages to breeders and breeding. Caused by DNA viruses of the family Poxviridae, genus Avipoxvirus, if manifest through 3 forms, cutaneous, diphtheric and septicemic. In June 2003, during illegal commercialization of Brazilian birds, 800 wild birds (Paroaria dominicana, Sporophila caerulescens and Sporophila albogularis) were apprehended and being forwarded to the CRAS (Wild Animals Recovery Center), Tietê Ecological Park. After one month, birds presented cutaneous lesions in the beak and feet and anorexia, emaciation, locomotion difficulties, diarrhea, dehydration and death. Among the 800 birds, 500 died and 40 these (15 Paroaria dominicana, 15 Sporophila...
Tipo: Journal article
Palavras-chave: Poxvirus; Paroaria dominicana; Sporophila caerulescens; Sporophila albogularis; Transmission electron microscopy.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200043

Ultrastructural Analysis of Masseter and Left Medial Pterigoid Muscles, After Unilateral Mandibular Molar Extraction: Experimental Study
Provedor de dados: 77
Autores: Benigno,Maria Ivone Mendes; Liberti,Edson Aparecido; Conde Jr,Airton Mendes; de Sousa Leal,Noélia Maria; Lopes Rodrigues,Karla Emanuelle; Mota,Vanessa Benigno; Amstalden,Eliane Maria Ingrid.
Twelve adult male Wistar rats (220 g average weight) were divided in 3 experimental groups: GI -15. GII ­ 30 and GIII ­ 60 days, after mandibular molar extraction, with three experimental animals and one control per group. Qualitatively, ultrastructural changes of protein filaments from myofibrils of these muscles and ipsilateral to the extractions were observed. Ultrastructure asymmetry and disorganization of Z line and I band, in the experimental group GII, of Medial Pterigoid muscle (MPT) were observed. The temporomandibular dysfunction, stimulated by the unilateral extractions of mandibular molars in rats may lead to modifications in the Z line and I band, which showed to be sensitive to this dysfunction. Changes in the MPT muscle, probably related to...
Tipo: Journal article
Palavras-chave: Skeletal muscular fibre; Masticator muscles; Occlusal alterations; Temporomandibular joint; Transmission electron microscopy.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200007

Viral Research in Brazilian Owls (Tyto alba and Rhinoptynx clamator) by Transmission Electron Microscopy
Provedor de dados: 77
Autores: Catroxo,M. H. B; Taniguchi,D. L; Melo,N. A; Milanelo,L; Petrella,S; Alves,M; Martins,A. M. C. R. P. F; Rebouças,M. M.
The barn-owl (Tyto Alba) and striped-owl (Rhinoptynx clamator) belong respectively to the families Tytonidae and Strigidae. Avian paramyxoviruses have been isolated from a variety of species of wild and domestic birds wordlwide causing diverse clinical symptoms and signs. Paramyxoviruses belong to the family Paramyxoviridae and Avulovirus genus, including nine serotypes (APMV 1 to 9). The lymphoid leukosis is a retrovirus-induced neoplasia. The avian retroviruses belong to the Retroviridae family and to the Alpharetrovirus genus. Coronaviruses can cause respiratory and enteric disease in several species of birds. They belong to the Coronaviridae family and to the groups 3a e 3c. In this study, we describe the presence of viruses in four owls, two barn owls...
Tipo: Journal article
Palavras-chave: Paramyxovirus; Coronavirus; Retrovirus; Owls; Transmission electron microscopy.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200047

Fine structural analysis of the stinger in venom apparatus of the scorpion Euscorpius mingrelicus (Scorpiones: Euscorpiidae)
Provedor de dados: 80
Autores: Yigit,N; Benli,M.
In this study, the morphology, histology and fine structure of the stinger, a part of the venom apparatus of Euscorpius mingrelicus (Kessler, 1874) (Scorpiones: Euscorpiidae) were studied by light microscopy and transmission electron microscopy (TEM). The stinger, located at the end section of the telson, is sickle-shaped. The venom is ejected through a pair of venom pores on its subterminal portion. Both venom ducts extend along the stinger without contact with each other since they are separated by connective tissue cells. The stinger cuticle is composed of two layers. Additionally, there are many pore canals and some hemolymph vessels in the cuticle. This work constitutes the first histological and fine structure study on Euscorpius mingrelicus stinger.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Euscorpius mingrelicus; Telson; Stinger; Fine structure; Transmission electron microscopy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100008

The host-parasite relationship between the rainbow trout (Oncorhynchus mykiss) and the ectoparasite Argulus foliaceus (Crustacea: Branchiura): epithelial mucous cell response, cortisol and factors which may influence parasite establishment
Provedor de dados: 16
Autores: Nolan, D.T.; Salm, A.L. van der; Wendelaar Bonga, S.E.
The effects of short-term infection with the branchurian crustacean ectoparasite Argulus foliaceus, and the fish stress hormone cortisol (which is reported to stimulate mucus discharge), were studied on the mucous cell population of the head skin of rainbow trout (Oncorhynchus mykiss). Argulus infection did not raise plasma cortisol significantly and had no effect on the number of epidermal mucous cells in the head skin. Cortisol was administered twice to groups of trout via the food, significantly elevating circulating plasma cortisol at 24 h post feeding without affecting numbers of mucous cells, and increasing the numbers of vesicles in the upper cells of the epidermis. Subsequent infection with the parasite (6 Argulus f/fish) did not affect either...
Tipo: Article / Letter to the editor
Palavras-chave: Ectoparasites; Host-parasite interaction; Skin epidermis; Cortisol; Mucous cells; Transmission electron microscopy.
Ano: 2000
URL: http://www.repository.naturalis.nl/record/534370

Morphological study of partridge Eimeria procera development in the foreign host - Leghorn chicks (Gallus gallus)
Provedor de dados: 19
Autores: Revajová, Viera; Loószová, Adriana; Goldová, Maria; Zibrín, Martin; Herich, Robert; Levkut, Mikulas.
In order to morphological study of non-specific host immune response, cross-species experimental infection of Leghorn chicks with partridge specific coccidium, Eimeria procera (106 oocyst/per birds) was done. Schizonts of E. procera were histologically observed in all the parts of small intestine and caeca of chicks. Heterophilic and lymphocytic infiltration in lamina propria, and damage of villi intestine were observed. Schizonts were studied by electron microscope after 12, 30, and 60 hours post-infection (h pi). Results demonstrated immature and structurally damaged schizonts at 12 h pi, delayed maturation of first generation schizonts at 30 h pi, and phagocytosis of parasites at 60 h pi by intraepithelial lymphocytes.
Palavras-chave: Eimeria procera; Transmission electron microscopy; Specific and non-specific host; Intraepithelial lymphocytes.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/144

Cryptosporidiosis and Isosporosis among AIDS Patients with Chronic Diarrhea in Thailand
Provedor de dados: 19
Autores: Wanachiwanawin, Darawan; Manatsathit, Sathaporn; Suwanagool, Parvinee; Tunsupasawasdikul, Somsith; Lertlaituan, Punpob; Thakerngpol, Kleophant.
Palavras-chave: Cryptosporidiosis; Isosporosis; AIDS patients; Chronic diarrhea; Transmission electron microscopy.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/274

Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
Provedor de dados: 63
Autores: Alves,Eduardo; Leite,Breno; Pascholati,Sérgio Florentino; Ishida,Maria Lúcia; Andersen,Peter Craig.
Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrastructure; Scanning electron microscopy; Transmission electron microscopy; Immunolabel.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200011

Cytopathology of callus cells infected with grapevine leafroll-associated virus 3
Provedor de dados: 102
Autores: SCAGLIUSI,SANDRA M. M.; VEGA,JORGE; KUNIYUKI,HUGO.
The cytopathology of grapevine (Vitis spp.) callus tissue infected with Grapevine leafroll-associated virus 3 (GLRaV-3), genus Vitivirus was studied in order to investigate the usefulness of callus cultures to study grapevine leafroll-associated viruses. Ultrathin sections were made from in vitro callus obtained from stems and shoots of GLRaV-3 infected grapevine plants. Callus was composed of two types of tissue. Translucent, soft callus was formed and composed of large loosely arranged cells, containing big vacuoles and a thin layer of cytoplasm. Other parts of the callus were brown-coloured and composed of small compactly arranged cells, which showed flexuous and rod-shaped closterovirus-like particles, with 10-12 nm in diameter, at higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Closterovirus; Vitis spp.; Transmission electron microscopy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000400008

Ocurrence of pepper yellow mosaic virus and cucumber mosaic virus on Capsicum chinense in the state of Amazonas, Brazil
Provedor de dados: 36
Autores: SOUZA,Leonor Cristina Silva; HANADA,Rogerio Eiji; ASSIS,Luiz Alberto Guimarães; CAMELO-GARCÍA,Viviana M.; REZENDE,Jorge Alberto Marques; YUKI,Valdir A.; KITAJIMA,Elliot W.
ABSTRACT The habanero chilli pepper, Capsicum chinense is an important crop in the Amazon Basin, mainly grown by small-scale producers. Capsicum chinense plants in an experimental field in the northern Brazilian state of Amazonas were found exhibiting characteristic symptoms of viral infection. Leaf sap from symptomatic plants examined under a transmission electron microscope revealed the presence of elongated flexuous particles and isometric particles. Using molecular assays, the viruses were identified as pepper yellow mosaic virus (PepYMV) and cucumber mosaic virus (CMV). Aphids, identified as Aphis gossypii, were found colonizing the C. chinense plants in the field and may be the vector for both PepYMV and CMV. We report the first occurrence of these...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Cucumovirus; Potyvirus; Transmission electron microscopy; RT-PCR; Aphis gossypii.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100005

Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã - doi: 10.4025/actasciagron.v34i4.14501
Provedor de dados: 1
Autores: Ribeiro, Luciene Oliveira; Universidade Federal de Lavras; Paiva, Luciano Vilela; Universidade Federal de Lavras; Pádua, Marlúcia Souza; Universidade Federal de Lavras; Santos, Breno Régis; Universidade Federal de Alfenas; Alves, Eduardo; Universidade Federal de Lavras; Stein, Vanessa Cristina; Universidade Federal de Goiás.
This work was carried out to characterise morphologically and ultrastructurally the banana callus, obtained from the scalp method. Genotypes of banana, cv. Prata anã, cultivated in vitro were used to induce meristems at the leaf base; subsequently, structures known as scalps were formed. For ultrastructural analysis, five samples of callus were collected, fixed in Karnovsky solution and analysed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The formation of three types of callus was observed: Type 1 - transparent watery callus, Type 2 - yellow callus with small clusters, Type 3 - yellow callus with large clusters. SEM analysis showed that Type 1 callus cells were elongated and that Type 2 and Type 3 callus cells were...
Tipo: Laboratory analysis
Palavras-chave: 5.01.00.00-9 Agronomia 5.01.00.00-9 scanning electron microscopy; Transmission electron microscopy; Musa sp.; Cytology; Embryogenesis Agronomia.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/14501

Ecophysiological, anatomical and ultrastructural characteristics of Vitex polygama Cham. (Verbenaceae) submitted to different concentrations of fluoride
Provedor de dados: 3
Autores: Rossi, Zélia Terezinha Teixeira; Castro, Evaristo Mauro de; Pereira, Giuslan Carvalho; Barbosa, João Paulo Rodrigues Alves Delfino; Carvalho, Graziele Wolff de Almeida; Rossi, Marco Aurélio de Lima Silva; Pereira, Fabrício José.
Fluorides released during the manufacture of aluminum, fertilizers, glass and ceramics are considered to be highly toxic to plants, causing damages in relatively small concentrations. This study aimed to evaluate fluoride (F) accumulation and its effects on Vitex polygama Cham. (Verbenaceae) exposed to simulated rain containing potassium fluoride in concentrations of 5, 10, 15 and 20 mg L-1. Vitex polygama presented no visual symptoms of foliar injury and accumulated a relatively small amount of F-, significant only for the treatments with higher concentrations of the pollutant.  F- promoted an increase in non-photochemical quenching (qN) and in the coefficient due to non-photochemical extinction (NPQ), which indicates higher dissipation of radiant energy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.03.00.00-0; 2.03.02.03-7; 2.03.03.00-9 chlorophyll a fluorescence; Potassium fluoride; Scanning electron microscopy; Transmission electron microscopy; Biomonitoring studies Botânica; Anatomia Vegetal; Fisiologia Vegetal.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31400

Anatomical and biochemical changes associated with the development and germination of Araucaria angustifolia seeds
Provedor de dados: 3
Autores: Shibata, Marília; Coelho, Cileide Maria Medeiros; Schmidt, Eder Carlos; Bouzon, Zenilda Laurita; Campos, José Marcello Salabert de; Maraschin, Marcelo.
This study aimed at assessing the cell cycle, and anatomical and biochemical changes that the embryonic axis of Araucaria angustifolia undergoes during development, focusing on the maturation stage. During all development, cells exhibited intense metabolic activity with an abundance of mitochondria, lipid bodies, and vacuolated cells. The continued accumulation of starch and protein was observed by LM and TEM and indicated by spectra of FTIR. Cell differentiation of the procambium was observed with a thickening of the cell wall and the formation of resiniferous ducts. At Stage III and IV, cells exhibited structural changes such as altered or elongated mitochondria and presence of plastoglobules. These results suggest that there is a gradual transition from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.03.00.00-0 Botânica; 2.03.03.00-9 Fisiologia Vegetal; 5.02.01.04-2 Sementes Florestais maturation; Recalcitrant seed; Light microscopy; Transmission electron microscopy; Flow cytometry Botânica.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43381

Deformation of Mitochondrial Cristae in Human Neural Progenitor Cells Exposed to Valproic Acid
Provedor de dados: 42
Autores: COSTA,RODRIGO M. DA; KARMIRIAN,KARINA; REHEN,STEVENS K.
ABSTRACT Neural development represents a dynamic process where mitochondrial integrity is decisive for neuronal activity. Structural changes in these organelles may be related to neurological disorders. Valproic acid (VPA) is an anticonvulsive drug commonly used for epilepsy treatment and its use is associated to increased risk of neuropsychiatric disorders. Recently we showed changes in shape and membrane potential in mitochondria from human neural progenitor cells (NPCs) exposed to VPA (da Costa et al. 2015). Here, we applied transmission electron microscopy and electron tomography to evaluate mitochondrial damage caused by VPA in NPCs. Results showed mitochondrial cristae disorganization in a dose dependent manner. Disturbance in mitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transmission electron microscopy; Mitochondria; Valproic acid; Epilepsy; Human neural progenitor cells; Electron tomography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502223

Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
Provedor de dados: 42
Autores: PáDUA,MARLÚCIA S.; PAIVA,LUCIANO V.; LABORY,CLAÚDIA R.G.; ALVES,EDUARDO; STEIN,VANESSA C.
ABSTRACTOil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses inE.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryogenic potential; Growth regulators; Tissue culture; Transmission electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401545

Structural analysis of flagellar axonemes from inner arm dynein knockdown strains of Trypanosoma brucei
Provedor de dados: 47
Autores: Zukas,Randi; Chang,Alex J.; Rice,Marian; Springer,Amy L.
Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the a heavy chain, and IC138, an intermediate chain, cause severe motility defects including immotility. To determine if motility defects result from structural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flagellar motility; Transmission electron microscopy; Central pair.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300006

Sensory nerve endings in the rat cheek mucosa: an electron microscopic study
Provedor de dados: 62
Autores: Watanabe,Ii-sei; Silva,Marcelo Cavenaghi Pereira da; Kronka,Marcia Consentino.
The sensory nerve endings of the rat cheek mucosa were studied using the transmission electron microscopy. The specimens were fixed in modified Karnovsky solution and embedded in Epon resin. The sensory nerve endings showed a central terminal axon containing numerous mitochondria, neurofilaments, microtubules and clear vesicles. The proximal part of corpuscle revealed the cytoplasmic extensions of lamellar cells and the perineural cells. The fine bundles of collagen fibers are identified in the interlamellar spaces and the external part of corpuscle. Numerous concentric lamellae showed caveolae, interlamelar spaces filled with amorphous material, desmosome-type junctions between adjacent lamellae and the inner lamellar cells and the axoplasmic membrane....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nerve endings; Lamellated corpuscle; Cheek mucosa; Transmission electron microscopy; Rat.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500001

Ultrastructural aspects in perithecia hyphae septal pores of Glomerella cingulata F. SP. Phaseoli
Provedor de dados: 58
Autores: Roca M.,María Gabriela; Ongarelli,Maria das Graças; Davide,Lisete Chamma; Mendes-Costa,Maria Cristina.
Glomerella cingulata (Stonem.) Spauld. &amp;amp; Schrenk f. sp. phaseoli, better known in its anamorphic state Colletotrichum lindemuthianum (Sacc. &amp;amp; Magn.) Briosi &amp;amp; Cav., is a causal agent of anthracnose in beans (Phaseolus vulgaris L.). Ultrastructural aspects of the perithecial hyphae of this pathogen were studied. The perithecia hyphae septal pores were found either plugged by a vesicle or unplugged. Some perithecia hyphae septa presented no pore. The Woronin bodies, close to the septal pores, appeared as globose structures which were more electron dense than the occlusions plugging the septal pore.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glomerella cingulata; Colletotrichum lindemuthianum; Transmission electron microscopy; Septal pores; Bean anthracnose.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300014

Mucoadhesive microemulsion of ibuprofen: design and evaluation for brain targeting efficiency through intranasal route
Provedor de dados: 60
Autores: Mandal,Surjyanarayan; Mandal,Snigdha Das; Chuttani,Krishna; Subudhi,Bharat Bhushan.
This study aimed at designing mucoadhesive microemulsion gel to enhance the brain uptake of Ibuprofen through intranasal route. Ibuprofen loaded mucoadhesive microemulsion (MMEI) was developed by incorporating polycarbophil as mucoadhesive polymer into Capmul MCM based optimal microemulsion (MEI) and was subjected to characterization, stability, mucoadhesion and naso-ciliotoxicity study. Brain uptake of ibuprofen via nasal route was studied by performing biodistribution study in Swiss albino rats. MEI was found to be transparent, stable and non ciliotoxic with 66.29 ± 4.15 nm, -20.9 ± 3.98 mV and 98.66 ± 1.01% as average globule size, zeta potential and drug content respectively. Transmission Electron Microscopy (TEM) study revealed the narrow globule size...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ibuprofen/brain-targeting; Ibuprofen/intranasal administration; Ibuprofen/mucoadhesion; Mucoadhesive microemulsion gel; Drugs/design; Transmission electron microscopy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300721

Antifungal activity and ultrastructural alterations in Pseudocercospora griseola treated with essential oils
Provedor de dados: 64
Autores: Hoyos,Julián Mauricio Ágredo; Alves,Eduardo; Rozwalka,Luciane Cristina; Souza,Elaine Aparecida de; Zeviani,Walmes Marques.
Pseudocercospora griseola, the etiologic agent of angular leaf spot of common bean (Phaseolus vulgaris), is an important disease in all bean-producing regions worldwide and may cause extremely high yield losses. The control of this disease is made more difficult by the pathogen's genetic variability and the inefficiency of fungicides. In this study, of 26 essential oils tested at different concentrations, 25 demonstrated efficiency in affecting the germination of strains 63-31 and 63-63 of the pathogen, reaching inhibition levels of between 80% and 100%. Cymbopogon citratus and Cymbopogon martinii inhibited conidia germination at all concentrations; Eugenia caryophyllata, Cinnamomum sp., Thymus vulgaris, Matricaria recutita, Cordia verbenacea, Origanum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angular leaf spot; Transmission electron microscopy; Alternative control of plant disease.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000300002

Essential oils for rust control on coffee plants
Provedor de dados: 64
Autores: Pereira,Ricardo Borges; Lucas,Gilvaine Ciavareli; Perina,Fabiano José; Alves,Eduardo.
Rust is considered the most important disease in coffee because it causes severe defoliation in plants and, consequently, reduction in productivity. This study evaluated the in vitro effect of essential oils of cinnamon, citronella, lemongrass, clove, tea tree, thyme, neem and eucalyptus on the germination of urediniospores of Hemileia vastatrix; the effectiveness of these oils to control rust on seedlings of coffee cultivars Catucaí 2SL, Catuaí IAC 62 and Mundo Novo 379/19 in the greenhouse; and the effect of more promising oils on urediniospores of H. vastatrix by transmission electron microscopy (TEM). All the essential oils inhibited the germination of urediniospores with increasing concentrations. All oils promoted partial control of the disease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemileia vastatrix; Alternative control; Transmission electron microscopy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000100002