Consulta: subjectFacets:"Transposable elements"
Registros recuperados: 17
Data/hora: 11/06/2024 14:50:56

Boty-II, a novel LTR retrotransposon in Botrytis cinerea B05.10 revealed by genomic sequence
Provedor de dados: 69
Autores: Zhao,Ming; Zhou,Jin yan; Li,Zhi dong; Song,Wei wei; Tan,You jiu; Tan,Hong.
Botrytis cinerea is a necrotrophic pathogen causing pre- and post-harvest diseases in at least 235 plant species. It manifests extraordinary genotype and phenotype variation. One of the causes of this variation is transposable elements. Two transposable elements have been discovered in this fungus, the retrotransposon (Boty), and the transposon (Flipper). In this work, two complete (Boty-II-76 and Boty-II-103) and two partial (Boty-II-95 and Boty-II-141) long terminal repeat (LTR) retrotransposons were identified by an in silico genomic sequence analysis. Boty-II-76 and Boty-II-103 contain 6439 bp nucleotides with a pair of LTRs at both ends, and an internal deduced pol gene encoding a polyprotein with reverse transcriptase and DDE integrase domains. They...
Tipo: Journal article
Palavras-chave: Botrytis cinerea; LTR retrotransposons; Molecular marker; Plant pathogen; Population diversity; Transposable elements.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300002

Evidence of ectopic recombination and a repeat-induced point (RIP) mutation in the genome of Sclerotinia sclerotiorum, the agent responsible for white mold
Provedor de dados: 74
Autores: Goldfarb,Míriam; Santana,Mateus Ferreira; Salomão,Tânia Maria Fernandes; Queiroz,Marisa Vieira de; Barros,Everaldo Gonçalves de.
Abstract Two retrotransposons from the superfamilies Copia and Gypsy named as Copia-LTR_SS and Gypsy-LTR_SS, respectively, were identified in the genomic bank of Sclerotinia sclerotiorum. These transposable elements (TEs) contained direct and preserved long terminal repeats (LTR). Domains related to codified regions for gag protein, integrase, reverse transcriptase and RNAse H were identified in Copia-LTR_SS, whereas in Gypsy-LTR_SS only domains for gag, reverse transcriptase and RNAse H were found. The abundance of identified LTR-Solo suggested possible genetic recombination events in the S. sclerotiorum genome. Furthermore, alignment of the sequences for LTR elements from each superfamily suggested the presence of a RIP (repeat-induced point mutation)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Phytopathogens; Retrotransposons; Transposable elements.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300426

A re-annotation of the Anopheles darlingi mobilome
Provedor de dados: 74
Autores: Diesel,Jose Francisco; Ortiz,Mauro F.; Marinotti,Osvaldo; Vasconcelos,Ana Tereza R.; Loreto,Elgion L. S.
Abstract The mobilome, portion of the genome composed of transposable elements (TEs), of Anopheles darlingi was described together with the genome of this species. Here, this mobilome was revised using similarity and de novo search approaches. A total of 5.6% of the A. darlingi genome is derived of TEs. Class I gypsy and copia were the most abundant superfamilies, corresponding to 22.36% of the mobilome. Non-LTR elements of the R1 and Jockey superfamilies account for 11% of the TEs. Among Class II TEs, the mariner superfamily is the most abundant (16.01%). Approximately 87% of the A. darlingi mobilome consist of short, truncated and/or degenerated copies of TEs. Only three retrotransposons, two belonging to gypsy and one to copia superfamilies, are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposable elements; Transposons; Mosquitoes; Evolvability.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100125

A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
Provedor de dados: 74
Autores: Deprá,Maríndia; Sepel,Lenira Maria Nunes; Loreto,Élgion Lucio da Silva.
We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenic Drosophila; PiggyBac; GFP; Transposable elements.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012

Drosophila relics hobo and hobo-MITEs transposons as raw material for new regulatory networks
Provedor de dados: 74
Autores: Loreto,Elgion L.S.; Deprá,Maríndia; Diesel,José F.; Panzera,Yanina; Valente-Gaiesky,Vera Lucia S.
Abstract Hypermutable strains of Drosophila simulans have been studied for 20 years. Several mutants were isolated and characterized, some of which had phenotypes associated with alteration in development; for example, showing ectopic legs with eyes being expressed in place of antennae. The causal agent of this hypermutability is a non-autonomous hobo-related sequence (hoboVA). Around 100 mobilizable copies of this element are present in the D. simulans genome, and these are likely mobilized by the autonomous and canonical hobo element. We have shown that hoboVA has transcription factor binding sites for the developmental genes, hunchback and even-skipped, and that this transposon is expressed in embryos, following the patterns of these genes. We suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hobo; Transposable elements; Cis-regulatory sequences; Drosophila.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200198

Unraveling the evolutionary scenario of the hobo element in populations of Drosophila melanogaster and D. simulans in South America using the TPE repeats as markers
Provedor de dados: 74
Autores: Ragagnin,Geovani T.; Bernardo,Larissa P.; Loreto,Elgion L. S.
Abstract Transposable elements (TEs) are nucleotide sequences found in most studied genomes. These elements are highly diversified and have a large variation in nucleotide structure and mechanisms of transposition. hobo is a member of class II, belonging to hAT superfamily, described inDrosophila melanogaster, and it presents in its Open Reading Frame, a repetitive region encoding the amino acids threonine-proline-glutamic acid (TPE), which shows variability in the number of repeats in some regions of the world. Due to this variability some evolutionary scenarios of the hobo element are discussed, such as the scenario of the invasion of hobo element in populations ofD. melanogaster. In the present study, we investigated 22 DNA sequences of D. melanogaster...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Transposable elements; Transposons; HAT; Genomic evolution; Molecular evolution.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100145

Transposable elements from the mesophragmatica group of Drosophila
Provedor de dados: 74
Autores: Germanos,Erika; Mota,Nina R.; Loreto,Elgion L.S.
Transposable elements (TEs) are middle repetitive DNA sequences classified into families according to their sequence similarities, such elements can playing an important role in the evolutionary process of their host genomes. There are many reports on the distribution of TEs in the fruit fly genus Drosophila, although there is relatively little information relating to the Neotropical mesophragmatica group of Drosophila, probably the most typical cluster of species occurring almost exclusively in the Andes mountains. Dot Blot and PCR analyses was used to study the distribution of some TEs (I, mariner, hobo, gypsy, Tom/17.6, micropia and P elements) within the mesophragmatica group of Drosophila. We found gypsy elements in all the mesophragmatica group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila; Mesophragmatica; Phylogenetic distribution; Transposable elements.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400026

Evolutionary history of the Tip100 transposon in the genus Ipomoea
Provedor de dados: 74
Autores: Christoff,Ana-Paula; Loreto,Elgion L.S.; Sepel,Lenira M.N.
Tip100 is an Ac-like transposable element that belongs to the hAT superfamily. First discovered in Ipomoea purpurea (common morning glory), it was classified as an autonomous element capable of movement within the genome. As Tip100 data were already available in databases, the sequences of related elements in ten additional species of Ipomoea and five commercial varieties were isolated and analyzed. Evolutionary analysis based on sequence diversity in nuclear ribosomal Internal Transcribed Spacers (ITS), was also applied to compare the evolution of these elements with that of Tip100 in the Ipomoea genus. Tip100 sequences were found in I. purpurea, I. nil, I. indica and I. alba, all of which showed high levels of similarity. The results of phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HAT; Transposable elements; Ac-Ds; Ipomoea; Genome evolution; ITS.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300013

Geographic polymorphism of P element in populations of Drosophila sturtevanti
Provedor de dados: 74
Autores: Almeida,Luciane M. de; Langeani,Francisco; Carareto,Claudia M.A.
The aim of this report was to detect full-sized P element sequences in eight strains of Drosophila sturtevanti populations from distant geographic regions and to assess the structural geographic variation among P element sequences. PCR analysis confirmed the presence of a putative complete P element in all strains. Southern blot analysis indicated bands shared by all strains, and bands restricted to geographically related strains. Parsimony analysis corroborated the hybridization pattern that reflected the geographic relationships.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canonical P elements; Drosophila; P element polymorphism; Saltans group; Transposable elements.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200010

Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum
Provedor de dados: 74
Autores: Kajihara,Daniela; Godoy,Fabiana de; Hamaji,Thais Alves; Blanco,Silvia Regina; Van Sluys,Marie-Anne; Rossi,Magdalena.
Transposable elements (TEs) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%-80% of the genomic content. TEs have recently been shown to be a source of new genes and new regulatory networks. The most striking contribution of TEs is referred as “molecular domestication”, by which the element coding sequence loses its movement capacity and acquires cellular function. Recently, domesticated transposases known as mustang and derived from the Mutator element have been described in sugarcane. In order to improve our understanding of the function of these proteins, we identified mustang genes from Sorghum bicolor and Zea mays and performed a phenetic analysis to assess the diversity and evolutionary history of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domesticated tranposases; Saccharum sp.; Sorghum bicolor; Transposable elements; Zea mays.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400014

New insights into Phakopsora pachyrhizi infection based on transcriptome analysis in planta
Provedor de dados: 74
Autores: Rincão,Michelle Pires; Carvalho,Mayra Costa da Cruz Gallo de; Nascimento,Leandro Costa; Lopes-Caitar,Valéria S.; Carvalho,Kenia de; Darben,Luana M.; Yokoyama,Alessandra; Carazzolle,Marcelo Falsarella; Abdelnoor,Ricardo Vilela; Marcelino-Guimarães,Francismar Correa.
Abstract Asian soybean rust (ASR) is one of the most destructive diseases affecting soybeans. The causative agent of ASR, the fungus Phakopsora pachyrhizi, presents characteristics that make it difficult to study in vitro, limiting our knowledge of plant-pathogen dynamics. Therefore, this work used leaf lesion laser microdissection associated with deep sequencing to determine the pathogen transcriptome during compatible and incompatible interactions with soybean. The 36,350 generated unisequences provided an overview of the main genes and biological pathways that were active in the fungus during the infection cycle. We also identified the most expressed transcripts, including sequences similar to other fungal virulence and signaling proteins. Enriched P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asian soybean rust; Multigenic families; Transposable elements.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400671

The biology and potential for genetic research of transposable elements in filamentous fungi
Provedor de dados: 74
Autores: Fávaro,Léia Cecilia de Lima; Araújo,Welington Luiz de; Azevedo,João Lúcio de; Paccola-Meirelles,Luzia Doretto.
Recently many transposable elements have been identified and characterized in filamentous fungi, especially in species of agricultural, biotechnological and medical interest. Similar to the elements found in other eukaryotes, fungal transposons can be classified as class I elements (retrotransposons) that use RNA and reverse transcriptase and class II elements (DNA transposons) that use DNA. The changes (transposition and recombination) caused by transposons can supply wide-ranging genetic variation, especially for species that do not have a sexual phase. The application of transposable elements to gene isolation and population analysis is an important tool for molecular biology and studies of fungal evolution.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposable elements; Filamentous fungi; Genetic application.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500024

Gonadal hybrid dysgenesis in Drosophila sturtevanti (Diptera, Drosophilidae)
Provedor de dados: 76
Autores: Almeida,Luciane M. de; Carareto,Claudia M. A.
The occurrence of hybrid dysgenesis was investigated in Drosophila sturtevanti Duda, 1927 using diagnostic crosses similar to those used for induction of dysgenics traits in D. melanogaster. Reciprocal test crosses were made, at 27° C, between an old laboratory strain of D. sturtevanti (COL, from Colombia), assumed to be an M'-like strain, and eight freshly collected strains from several natural populations. The gonadal dysgenesis indices were under 10% in most of crosses, except in hybrids of COL with I27, a strain from Minas Gerais (Brazil), in which the index values were moderate in both directions of crosses (25.71 and 12.87). The smallest productivity was also observed in hybrids of females COL mated to I27 males. No causal relationship between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hybrid sterility; P element; I element; Hobo element; Transposable elements.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212002000200007

A transposable element annotation pipeline and expression analysis reveal potentially active elements in the microalga Tisochrysis lutea
Provedor de dados: 5
Autores: Berthelier, Jeremy; Casse, Nathalie; Daccord, Nicolas; Jamilloux, Veronique; Saint-jean, Bruno; Carrier, Gregory.
Background Transposable elements (TEs) are mobile DNA sequences known as drivers of genome evolution. Their impacts have been widely studied in animals, plants and insects, but little is known about them in microalgae. In a previous study, we compared the genetic polymorphisms between strains of the haptophyte microalga Tisochrysis lutea and suggested the involvement of active autonomous TEs in their genome evolution. Results To identify potentially autonomous TEs, we designed a pipeline named PiRATE (Pipeline to Retrieve and Annotate Transposable Elements, download: https://doi.org/10.17882/51795), and conducted an accurate TE annotation on a new genome assembly of T. lutea. PiRATE is composed of detection, classification and annotation steps. Its...
Tipo: Text
Palavras-chave: Transposable elements; Genome assembly; Pipeline; Tool; Annotation; Algae; Haptophyte; Tisochrysis lutea.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00441/55250/56732.pdf

Chromosomal distribution of the retroelements Rex 1, Rex 3 and Rex 6 in species of the genus Harttia and Hypostomus (Siluriformes: Loricariidae)
Provedor de dados: 83
Autores: Traldi,Josiane B.; Lui,Roberto L.; Martinez,Juliana de F.; Vicari,Marcelo R.; Nogaroto,Viviane; Moreira Filho,Orlando; Blanco,Daniel R.
ABSTRACT The transposable elements (TE) have been widely applied as physical chromosome markers. However, in Loricariidae there are few physical mapping analyses of these elements. Considering the importance of transposable elements for chromosomal evolution and genome organization, this study conducted the physical chromosome mapping of retroelements (RTEs) Rex1, Rex3 and Rex6 in seven species of the genus Harttia and four species of the genus Hypostomus, aiming to better understand the organization and dynamics of genomes of Loricariidae species. The results showed an intense accumulation of RTEs Rex1, Rex3 and Rex6 and dispersed distribution in heterochromatic and euchromatic regions in the genomes of the species studied here. The presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Armored catfish; Fluorescence in situ hybridization; Hypostominae; Loricariinae; Transposable elements.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000200214

PCR amplification and sequence analyses of reverse transcriptase-like genes in Crinipellis perniciosa isolates
Provedor de dados: 102
Autores: Pereira,Jorge F.; Ignacchiti,Mariana D.C.; Araújo,Elza F.; Brommonschenkel,Sérgio H.; Cascardo,Júlio C.M.; Pereira,Gonçalo A. G.; Queiroz,Marisa V.
Reverse transcriptase (RT) sequence analysis is an important technique used to detect the presence of transposable elements in a genome. Putative RT sequences were analyzed in the genome of the pathogenic fungus C. perniciosa, the causal agent of witches' broom disease of cocoa. A 394 bp fragment was amplified from genomic DNA of different isolates of C. perniciosa belonging to C-, L-, and S-biotypes and collected from various geographical areas. The cleavage of PCR products with restriction enzymes and the sequencing of various RT fragments indicated the presence of several sequences showing transition events (G:C to A:T). Southern blot analysis revealed high copy numbers of RT signals, forming different patterns among C-, S-, and L-biotype isolates....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Transposable elements; Witches' broom; Theobroma cacao.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500001

Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP
Provedor de dados: 52
Autores: Finokiet,Manuela; Goni,Beatriz; Loreto,Élgion Lúcio Silva.
Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposable elements; Insect transgenesis; Germ-line transformation; Fluorescent protein marker; Microinjection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013