Consulta: subjectFacets:"Triploid induction"
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Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe.
A novel method using 6-dimcthylaminopurinc (6-0MAP) has recently been demonstrated to be a good triploid inductor for bivalve molluscs. The effects of 6-DMAP concentration, timing of treatment after fertilization and treatment duration on survival and triploid induction of Crassostrea gigas (Thunberg) embryos were examined. An influence of sperm concentration was also detected and discussed. Survival to D-stage was inversely related to 6-DMAP concentration and the percentage of triploids was shown to be 6-DMAP dose dependent. Two optimal moments for treatment application were determined corresponding to extrusions of the first and second polar bodies. Optimal treatment of 450 µmol/l 6-DMAP beginning 15 min after fertilization over a 10 min period yielded...
Tipo: Text
Palavras-chave: Genetic; Triploid induction; Crassostrea gigas; 6 DMAP; Triploid; Oysters.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-3198.pdf