Consulta: subjectFacets:"Type II collagen"
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Data/hora: 02/06/2024 00:47:39

Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits
Provedor de dados: 45
Autores: Filgueiras,R.R.; Del Carlo,R.J.; Alves,N.P.; Viloria,M.I.V.; McManus,C.M.; Castro,M.B.; Filgueiras,F.P.F.; Monteiro,B.S.; Almeida,R.M.; Andrade,R.R.
Chondrocytes obtained from stifle joint of New Zealand White rabbits were cultivated. Half of cells were maintained in culture for later implantation and the others frozen during six months to evaluate viability. A circular osteochondral defect was created in the right stifle of other twenty seven rabbits. The control group (CG) received no treatment. The thawed (TH) and fresh (FH) heterologous groups received, respectively, an implant of cultivated thawed or fresh heterologous chondrocytes associated with platelet rich plasma (PRP). The CG group showed greatest pain and lameness compared to the other groups seven days after the implantation. Microscopically, at 45 and 90 days, the TH and FH groups showed filling with cartilaginous tissue containing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabbits; Articular cartilage; Chondrocyte implantation; Platelet rich plasma; Type II collagen.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100024

Effect of freezing on rabbit cultured chondrocytes
Provedor de dados: 45
Autores: Filgueiras,R.R; Del Carlo,R.J; Alves,N.P; Viloria,M.I.V; McManus,C.M; Castro,M.B; Filgueiras,F.P.F; Monteiro,B.S; Paula,A.C; Farias,A.
This work evaluated the effect of freezing on chondrocytes maintained in culture, aiming the establishment of a cell bank for future application as heterologous implant. Chondrocytes extracted from joint cartilage of nine healthy New Zealand White rabbits were cultivated and frozen with the cryoprotector 5% dimethylsulfoxide for six months. Phenotypic and scanning electron microscopy analyses were carried out to identify morphological and functional differences between fresh and thawed cells. After enzymatic digestion, a total of 4.8x10(5)cells per rabbit were obtained. Fresh chondrocytes showed a high mitotic rate and abundant matrix was present up to 60 days of culture. Loss of phenotypic stability was notable in the thawed chondrocytes, with a low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabbit; Cell freezing; Cell therapy; Joint cartilage; Type II collagen.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100008

Cotransfected human chondrocytes: over-expression of IGF-I and SOX9 enhances the synthesis of cartilage matrix components collagen-II and glycosaminoglycans
Provedor de dados: 56
Autores: Simental-Mendía,M.; Lara-Arias,J.; Álvarez-Lozano,E.; Said-Fernández,S.; Soto-Domínguez,A.; Padilla-Rivas,G. R.; Martínez-Rodríguez,H. G.
Damage to cartilage causes a loss of type II collagen (Col-II) and glycosaminoglycans (GAG). To restore the original cartilage architecture, cell factors that stimulate Col-II and GAG production are needed. Insulin-like growth factor I (IGF-I) and transcription factor SOX9 are essential for the synthesis of cartilage matrix, chondrocyte proliferation, and phenotype maintenance. We evaluated the combined effect of IGF-I and SOX9 transgene expression on Col-II and GAG production by cultured human articular chondrocytes. Transient transfection and cotransfection were performed using two mammalian expression plasmids (pCMV-SPORT6), one for each transgene. At day 9 post-transfection, the chondrocytes that were over-expressing IGF-I/SOX9 showed 2-fold increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type II collagen; Glycosaminoglycans; IGF-I/SOX9 transgenes; Human chondrocytes; Articular cartilage; Cotransfection.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201063

Adhesion of the human pathogen Sporothrix schenckii to several extracellular matrix proteins
Provedor de dados: 56
Autores: Lima,O.C.; Figueiredo,C.C.; Pereira,B.A.S.; Coelho,M.G.P.; Morandi,V.; Lopes-Bezerra,L.M.
The pathogenic fungus Sporothrix schenckii is the causative agent of sporotrichosis. This subcutaneous mycosis may disseminate in immunocompromised individuals and also affect several internal organs and tissues, most commonly the bone, joints and lung. Since adhesion is the first step involved with the dissemination of pathogens in the host, we have studied the interaction between S. schenckii and several extracellular matrix (ECM) proteins. The binding of two morphological phases of S. schenckii, yeast cells and conidia, to immobilized type II collagen, laminin, fibronectin, fibrinogen and thrombospondin was investigated. Poly (2-hydroxyethyl methacrylate) (poly-HEMA) was used as the negative control. Cell adhesion was assessed by ELISA with a rabbit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sporothrix schenckii; Fibronectin; Laminin; Type II collagen; Adhesion.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500020