Consulta: subjectFacets:"Typing"
Registros recuperados: 8
Data/hora: 01/06/2024 01:18:40

Molecular epidemiology of Vibrio nigripulchritudo, a pathogen of cultured penaeid shrimp (Litopenaeus stylirostris) in New Caledonia
Provedor de dados: 5
Autores: Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Saulnier, Denis; Leroux, Frederique.
A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into this possibly emerging pathogen. Additionally, results obtained with the two molecular typing techniques were congruent and allowed discriminating the strains associated with the "Summer Syndrome" from strains isolated from other contexts, especially the other shrimp...
Tipo: Text
Palavras-chave: Virulence; Epidemiology; Typing; MLST; AP PCR; Mariculture; Vibriosis.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1913.pdf

Potential use of molecular-typing methods for the identification and characterization of salmonella enterica serotypes isolated in the poultry production chain
Provedor de dados: 89
Autores: Baratto,CM; Gelinski,JMLN; Bernardi,AZ; Marafon,A; Braun,F.
Salmonella is widespread in nature and can be found in all links of the poultry production chain. Due to its high impact on meat processing, techniques for the rapid detection and reproducible characterization of Salmonella serotypes in foods are needed. The present study investigated the potential of molecular profiling to identify and differentiate 15 Salmonella serotypes isolated from the poultry production chain, based on 5 primers by random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC-PCR), amplification of rDNA internal spacer analysis (RISA), and amplified ribosomal DNA restriction analysis (ARDRA) of 16S-23S rRNA internal spacer region (ISR) cleaved with Alu I and Hha I restriction enzymes. Three isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular analysis; Salmonella; Serotypes; Typing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000300003

Diagnosis of infectious bronchitis: an overview of concepts and tools
Provedor de dados: 89
Autores: Villarreal,LYB.
Infectious bronchitis (IB) casues multi-systemic infection in chickens with signs similar caused by other poultry pathogens and thus a high diagnostic accuracy can only be achieved by s series of laboratory assays. This article reviews in a brief way the traditional virus assays such as embryo innoculation, tracheal rings and virus neutralization assays for the direct detection of Avian infectious bronchitis virus (IBV) and methods based on gene molecular biology and some assays for the detection of anti-IBV antibodies, including ELISA. A critical view on each technique is also provived by the author.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Diagnosis; IBV; Typing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200006

Typing class I HLA-A gene using a nested PCR-RFLP procedure
Provedor de dados: 56
Autores: Castelli,E.C.; Gil,D.S.; Veiga,L.C.S.; de Camargo,J.L.V.
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human leukocyte antigen-A; HLA-A; Typing; RFLP; Polymorphism; Major histocompatibility complex.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600004

Typing of Pseudomonas aeruginosa from hospitalized patients: a comparison of susceptibility and biochemical profiles with genotype
Provedor de dados: 56
Autores: Freitas,A.L.; Barth,A.L.
Typing techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the "gold standard" for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to perform and to interpret and relatively inexpensive. Antibiotypes (susceptibility profiles) and biotypes (biochemical profiles) were compared to genotypes established by DNA restriction enzyme analysis in 81 clinical isolates of P. aeruginosa from three hospitals in Porto Alegre, Brazil. The epidemiological relationship among patients was also evaluated. Susceptibility and restriction profiles were discrepant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Typing; Genotyping; Phenotypic markers.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100011

First identification of Mycobacterium avium paratuberculosis sheep strain in Argentina
Provedor de dados: 58
Autores: Travería,G.E.; Zumarraga,M.; Etchechoury,I.; Romano,M.I.; Cataldi,A.; Alvarado Pinedo,M.F.; Pavlik,I.; Pribylova,R.; Romero,J.R.
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Sheep; Typing; PCR; PCR-REA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300037

Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA)
Provedor de dados: 58
Autores: Bustamante,Ana V.; Lucchesi,Paula M.A.; Parma,Alberto E.
The aim of this work was to adapt described MLVA protocols to the molecular typing and characterization of VTEC O157:H7 isolates from Argentina. Nine VNTR loci were amplified by PCR showing diversity values from 0.49 to 0.73. Nine MLVA profiles were observed and the cluster analysis indicated both unrelated and closely related VTEC O157:H7 strains. In spite of the limited number of isolates studied, the panel of VNTR used made it possible to perform a first approach of the high genetic diversity of native strains of O157:H7 by MLVA.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MLVA; VTEC O157:H7; Molecular characterization; Typing.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400026

Typing of Streptococcus mutans strains isolated from caries free and susceptible subjects by multilocus enzyme electrophoresis
Provedor de dados: 58
Autores: Tahmourespour,Arezoo; Nabinejad,Abdolreza; Shirian,Hannaneh; Rosa,Edvaldo Antonio Ribeiro; Tahmourespour,Sanaz.
This study was evaluated the clonal diversity of Streptococcus mutans in caries-free and caries-active subjects using MLEE. Strains from caries-free subjects were grouped in a single taxon. Unrooted dendrogram showed that different strains clustered in four different clades, also showed that more than one clonal type can be found in a same individual.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus mutans; Multilocus enzyme electrophoresis; Caries free; Caries susceptible; Typing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300033