Consulta: subjectFacets:"VNTR"
Registros recuperados: 7
Data/hora: 01/06/2024 10:35:28

PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
Provedor de dados: 74
Autores: Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Genetic diversity; Shrimp; VNTR; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012

A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors
Provedor de dados: 5
Autores: Nasfi, H.; Travers, Marie-agnes; De Lorgeril, Julien; Habib, C.; Sannie, T.; Sorieul, L.; Gerard, J.; Avarre, J. C.; Haffner, Philippe; Tourbiez, Delphine; Renault, Tristan; Furones, D.; Roque, A.; Pruzzo, C.; Cheslett, D.; Gdoura, R.; Vallaeys, T.
The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase...
Tipo: Text
Palavras-chave: VNTR; Vibrio splendidus; Epidemiology; Vsm; OmpU; Crassotrea giga; Mortality.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00255/36666/35350.pdf

Diversidad genética de Mycoplasma hyopneumoniae en granjas porcinas de Argentina
Provedor de dados: 78
Autores: Tamiozzo,P.J; Lucchesi,P.M.A; Ambrogi,A.
El Mycoplasma hyopneumoniae es el agente causal de la neumonía enzoótica porcina (NEP), una de las enfermedades respiratorias de mayor impacto en la industria porcina. Conocer su diversidad genética permite el desarrollo de estudios epidemiológicos y de patogénesis para desarrollar estrategias de control más efectivas. En este trabajo se analizaron 39 muestras de ADN de hisopado nasal y lavado bronquial de cerdos de distintas edades, provenientes de siete orígenes diferentes, que habían sido positivas a la detección de M. hyopneumoniae por nPCR. Para tipificar este patógeno en las muestras provenientes de hisopados nasales, se diseñó una nPCR que amplifica en el gen p146 una región que codifica serinas repetidas en tándem. Para las muestras de lavados...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma hyopneumoniae; VNTR; Gen p146; Tipificación.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982011000100003

Association of number of tandem repeats in two important adhesins in Mycoplasma hyopneumoniae
Provedor de dados: 45
Autores: Santos,L. F. dos; Alvarez,J.; Perez,A.; Moreira,M. A. S.; Pieters,M.
RESUMODiversidade genética de Mycoplasma hyopneumoniae tem sido relatada em análise múltipla de repetições em tandem em número variável (MLVA). O objetivo deste estudo foi descrever a distribuição espacial e a heterogeneidade genética de tipos de M. hyopneumoniae no Brasil, bem como investigar a correlação entre regiões de repetição 1 (RR1) e 3 (RR3) de duas adesinas importantes (P97 e P146). Foram identificados 39 tipos de MLVA baseados no número de repetições em tandem em P97 RR1 e RR3 P146. A correlação negativa significativa (Spearman's rho = -0,26; P = 0,022) entre P97 RR1 e RR3 P146 foi observada, o que sugere um possível mecanismo compensatório que permitiria a bactéria manter a sua capacidade de adesão. Os resultados contribuem para compreender a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Adhesion; VNTR; MLVA; Swine; Enzootic pneumonia.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000501461

Variable number tandem repeats in the promoter region of prostacyclin synthase gene in choline deficient rats
Provedor de dados: 47
Autores: Denninghoff,Valeria C.; Ossani,Georgina P.; Uceda,Ana M.; Avagnina,Maria A.; Elsner,Boris; Monserrat,Alberto J.
Weanling Sprague-Dawley rats were fed on a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Pathogenesis of the latter is controversial and an ischemic mechanism has been proposed. Arachidonic acid derivatives are involved in the regulation of vascular tonus. Vasospasm could be due to an increase in tromboxane A2-mediated vasoconstriction or to a decrease in prostacyclin-induced vasodilatation. Enzymes involved in the synthesis of both compounds are tromboxane A2- and prostacyclin-synthase respectively. The aim of this study was to identify the variable number tandem repeats (VNTR) in the promoter region of prostacyclin synthase gene and verify if there exists a relationship between the occurrence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: VNTR; Prostacyclin synthase gene; Kidney.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200002

Characterization of a virulent Leptospira interrogans strain isolated from an abandoned swimming pool
Provedor de dados: 58
Autores: Forster,Karine M.; Hartwig,Daiane D.; Seixas,Fabiana K.; McBride,Alan J.A.; Monte,Leonardo G.; Recuero,Ana Lúcia C.; Brod,Claudiomar S.; Hartleben,Cláudia P.; Amaral,Marta; Dellagostin,Odir A.
Pathogenic Leptospira spp. are the etiological agents of leptospirosis, an important disease of both humans and animals. In urban settings, L. interrogans serovars are the predominant cause of disease in humans. The purpose of this study was to characterize a novel Leptospira isolate recovered from an abandoned swimming pool. Molecular characterization through sequencing of the rpoB gene revealed 100% identity with L. interrogans and variable-number tandem-repeat (VNTR) analysis resulted in a banding pattern identical to L. interrogans serogroup Icterohaemorrhagiae, serovar Copenhageni or Icterohaemorrhagiae. The virulence of the strain was determined in a hamster model of lethal leptospirosis. The lethal dose 50% (LD50) was calculated to be two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Leptospirosis; Virulent; VNTR; RpoB.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100024

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
Provedor de dados: 58
Autores: Miraglia,Fabiana; Moreno,Andréa Mike; Gomes,Cleise Ribeiro; Paixão,Renata; Liuson,Esequiel; Morais,Zenaide Maria; Maiorka,Paulo; Seixas,Fabiana Kömmling; Dellagostin,Odir Antonio; Vasconcellos,Silvio Arruda.
With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers &gt; 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pomona; Swine; VNTR; Culture; PCR; Genital Tract.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017