Consulta: subjectFacets:"Venomics"
Registros recuperados: 7
Data/hora: 01/06/2024 12:46:09

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity
Provedor de dados: 80
Autores: Erdeş,Efe; Doğan,Tuğba Somay; Coşar,İlhan; Danışman,Tarık; Kunt,Kadir Boğaç; Şeker,Tamay; Yücel,Meral; Özen,Can.
Background Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs.Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.Methods In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.Results Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Toxin; Peptide; Leiurus abdullahbayrami; Microfluidic capillary; Electrophoresis; Peptidomics; Venomics; Cytotoxicity; Antimicrobial activity; Turkey.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200338

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms
Provedor de dados: 80
Autores: Lomonte,Bruno; Calvete,Juan J.
Abstract This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; Proteomics; Venomics; Antivenomics; Toxicovenomics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100206

Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening
Provedor de dados: 80
Autores: El-Aziz,Tarek Mohamed Abd; Khoury,Sawsan Al; Jaquillard,Lucie; Triquigneaux,Mathilde; Martinez,Guillaume; Bourgoin-Voillard,Sandrine; Sève,Michel; Arnoult,Christophe; Beroud,Rémy; Waard,Michel De.
Abstract  Background Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms should be rich enough in sperm-modulating compounds for a drug discovery program. Our objective was to demonstrate this fact by using a sperm-based phenotypic screening to identify positive modulators from the venom of Walterinnesia aegyptia.  Methods Herein, as proof of concept that venoms contain interesting compounds for sperm physiology, we fractionated Walterinnesia aegyptia snake venom by RP-HPLC and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Walterinnesia aegyptia; Bioactive compounds; Fertility; Sperm motility; Venomics; Tandem mass spectrometry; De novo sequencing; Edman degradation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100301

Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia
Provedor de dados: 80
Autores: Lingam,Thava Malar Changra; Tan,Kae Yi; Tan,Choo Hock.
Background The Eastern Russell’s viper, Daboia siamensis, is a WHO Category 1 medically important venomous snake. It has a wide but disjunct distribution in Southeast Asia. The specific antivenom, D. siamensis Monovalent Antivenom (DsMAV-Thailand) is produced in Thailand but not available in Indonesia, where a heterologous trivalent antivenom, Serum Anti Bisa Ular (SABU), is used instead. This study aimed to investigate the geographical venom variation of D. siamensis from Thailand (Ds-Thailand) and Indonesia (Ds-Indonesia), and the immunorecognition of the venom proteins by antivenoms.  Methods:  The venom proteins were decomplexed with reverse-phase high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venomics; Antivenomics; Eastern Russell's viper; Neutralization.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100302

It is time for top-down venomics
Provedor de dados: 80
Autores: Melani,Rafael D.; Nogueira,Fabio C. S.; Domont,Gilberto B.
Abstract The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (&gt; 50 kDA) and protein interactions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venomics; Toxiforms; Top-down proteomics; Denaturing top-down proteomics; Native top-down proteomics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100209

Venomics and antivenomics of the poorly studied Brazil’s lancehead, Bothrops brazili (Hoge, 1954), from the Brazilian State of Pará
Provedor de dados: 80
Autores: Sanz,Libia; Pérez,Alicia; Quesada-Bernat,Sarai; Diniz-Sousa,Rafaela; Calderón,Leonardo A.; Soares,Andreimar M.; Calvete,Juan J.; Caldeira,Cleópatra A. S.
ABSTRACT  Background: The Brazil’s lancehead, Bothrops brazili, is a poorly studied pit viper distributed in lowlands of the equatorial rainforests of southern Colombia, northeastern Peru, eastern Ecuador, southern and southeastern Venezuela, Guyana, Suriname, French Guiana, Brazil, and northern Bolivia. Few studies have been reported on toxins isolated from venom of Ecuadorian and Brazilian B. brazili. The aim of the present study was to elucidate the qualitative and quantitative protein composition of B. brazili venom from Pará (Brazil), and to carry out a comparative antivenomics assessment of the immunoreactivity of the Brazilian antibothropic pentavalent antivenom [soro antibotrópico (SAB) in Portuguese] against the venoms of B. brazili and reference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops brazili; Venomics; Third-generation antivenomics; Brazilian antibothropic polyvalent antivenom.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100308

Protein structure of the venom in nine species of snake: from bio-compounds to possible healing agents
Provedor de dados: 56
Autores: Cristina,R.T.; Kocsis,R.; Tulcan,C.; Alexa,E.; Boldura,O.M.; Hulea,C.I.; Dumitrescu,E.; Radulov,I.; Muselin,F.
Due to its various structures in bio-compounds, snake venom is the indisputable result of evolutionary stages of molecules with an increasingly complex structure, high specificity, and of great importance for medicine because of their potential. The present study proposed an underpinning examination of venom composition from nine species of venomous snakes using a useful and replicable methodology. The objective was the extension of the evaluation of protein fractions in the field up to 230 kDa to permit possible identification of some fractions that are insufficiently studied. The gel capillary electrophoresis method on the chip was performed using an Agilent 2100 bioassay with the 80 and 230-LabChip Protein kits. Interpretation of electrophoresis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bio-compounds; Protein LabChip; Venomics; Vipera aspis; V. xantina palestinae.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100610