Consulta: subjectFacets:"Vibrio parahaemolyticus"
Registros recuperados: 11
Data/hora: 01/06/2024 08:45:44

Amplification of tlh gene in other Vibrionaceae specie by specie-specific multiplex PCR of Vibrio parahaemolyticus
Provedor de dados: 69
Autores: Yáñez,Romina; Bastías,Roberto; Higuera,Gastón; Salgado,Oscar; Katharios,Pantelis; Romero,Jaime; Espejo,Romilio; García,Katherine.
Background The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification of three different hemolysin genes, which are specie-specific virulence factors. These genes are also employed in the determination of V. parahaemolyticus pathogenic load in seafood and for characterization of pathogenic strains associated to diarrhea cases in human. During environmental surveillance that we performed every summer, we occasionally observed a thermolabile hemolysin (tlh) PCR product of a slightly smaller size than expected, which was coincident with low loads of V. parahaemolyticus in the environment. In order to understand this observation, we probed the specificity of tlh primers for the detection of V....
Tipo: Journal article
Palavras-chave: Hemolysin; Multiplex PCR; Pathogen surveillance; Virulence factor; Vibrio parahaemolyticus.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600012

Bactéries pathogènes indigènes des eaux estuariennes: cas des vibrios. Revue bibliographique
Provedor de dados: 5
Autores: Fouche, Dominique.
De nombreuses bactéries, peuplant habituellement les zones estuariennes, se révèlent être de plus en plus fréquemment des agents de maladies dont les vecteurs principaux sont les coquillages et leur consommation par l'homme. Le présent travail synthétise l'état des connaissances sur les vibrios, en particulier Vibrio cholerae, Vibrio parahaemolyticus et Vibrio vulnificus, bactéries pour lesquelles il existe une  abondante littérature. L'écologie, le pouvoir pathogène et les milieux de culture les plus couramment utilisés en vue de leur détection sont présentés. Enfin, leur interaction avec les mollusques bivalves, leur survie dans ceux-ci et le problème de l'épuration conséquente de ces derniers sont abordés.
Tipo: Text
Palavras-chave: Vibrio cholerae; Vibrio parahaemolyticus; Vibrio vulnificus; Bactéries indigènes; Estuaires; Bivalves; Purification.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00408/51929/52554.pdf

MRNA detection by reverse transcription-PCR for monitoring viability and potential virulence in a pathogenic strain of Vibrio parahaemolyticus in viable but nonculturable state
Provedor de dados: 5
Autores: Coutard, Francois; Pommepuy, Monique; Loaec, Solen; Hervio Heath, Dominique.
Aims: This work investigates the maintenance of viability and potential virulence of Vibrio parahaemolyticus in a viable but nonculturable population (VBNC) state by reverse transcription-polymerase chain reaction (RT-PCR).   Methods and Results: Housekeeping genes, 16S-23S rDNA and rpoS, as well as virulence genes, tdh1 and tdh2, were selected and detected by PCR in a pathogenic strain of V. parahaemolyticus (Vp4). Their expression was then studied by RT-PCR in V. parahaemolyticus Vp4 cultivated in rich medium at 37degreesC. The 16S-23S rDNA and rpoS, tdh1, tdh2 genes were transcripted at the mid-logarithmic, stationary and late stationary phases, corresponding to various physiological states. The expression of these genes was also studied by RT-PCR in a...
Tipo: Text
Palavras-chave: Vibrio parahaemolyticus; Viable but nonculturable state; Tdh2; Tdh1; RT PCR; RpoS; Environment; 16S 23S rDNA.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1098.pdf

Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation?
Provedor de dados: 5
Autores: Coutard, Francois; Crassous, Philippe; Droguet, Michael; Gobin, Eric; Colwell, Rita; Pommepuy, Monique; Hervio Heath, Dominique.
The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population...
Tipo: Text
Palavras-chave: Transmission electron microscopy; Scanning electron microscopy; Resuscitation; Regrowth; Vibrio parahaemolyticus; VBNC state.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3117.pdf

Epidemiological investigation of a foodborne outbreak in Spain associated with U.S. West Coast genotypes of Vibrio parahaemolyticus
Provedor de dados: 5
Autores: Martinez-urtaza, Jaime; Powell, Andy; Jansa, Josep; Castro Rey, Jose Luis; Paz Montero, Oscar; Garcia Campello, Marta; Zamora Lopez, Ma Jose; Pousa, Anxela; Faraldo Valles, Ma Jose; Trinanes, Joaquin; Hervio-heath, Dominique; Keay, William; Bayley, Amanda; Hartnell, Rachel; Baker-austin, Craig.
We describe an outbreak of seafood-associated Vibrio parahaemolyticus in Galicia, Spain in on 18th of August 2012 affecting 100 of the 114 passengers travelling on a food banquet cruise boat. Epidemiological information from 65 people was available from follow-on interviews, of which 51 cases showed symptoms of illness. The food items identified through the questionnaires as the most probable source of the infections was shrimp. This product was unique in showing a statistically significant and the highest OR with a value of 7.59 (1.52–37.71). All the nine strains isolated from stool samples were identified as V. parahaemolyticus, seven were positive for both virulence markers tdh and trh, a single strain was positive for trh only and the remaining strain...
Tipo: Text
Palavras-chave: Seafood; Tdh; Trh; Vibrio parahaemolyticus; PNW clone; ST 36.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00311/42181/41482.pdf

The use of API 20E for the identification of &lt;i&gt;Vibrio anguillarum&lt;/i&gt; and &lt;i&gt;V. ordalii&lt;/i&gt;
Provedor de dados: 27
Autores: Grisez, L.; Ceusters, R.; Ollevier, F.P.
Sixty-eight strains of &lt;i&gt;Vibrio anguillarum&lt;/i&gt;, five of &lt;i&gt;V. ordalii&lt;/i&gt; and the type strains of &lt;i&gt;V. alginolyticus, V. carchariae, V. damsela&lt;/i&gt; and &lt;i&gt;V. parahaemolyticus&lt;/i&gt; were compared using the API 20E gallery. Within the &lt;i&gt;V. anguillarum&lt;/i&gt; strains, distinct groups could be separated mainly on the basis of their reaction on indole production and the fermentation of amygdalin and arabinose. &lt;i&gt;Vibrio ordalii&lt;/i&gt;, the former &lt;i&gt;V. anguillarum&lt;/i&gt; biotope 2, could easily be separated from &lt;i&gt;V. anguillarum&lt;/i&gt; and from the other fish pathogenic &lt;i&gt;Vibrio&lt;/i&gt; spp.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial diseases; Disease control; Disease detection; Fish diseases; Identification; Vibrio carchariae; Vibrio ordalii; Vibrio alginolyticus; Vibrio anguillarum; Vibrio damsela; Vibrio parahaemolyticus.
Ano: 1991
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2859

Avaliação de parâmetros físicos e químicos e pesquisa de Vibrio parahaemolyticus em águas do estuário do rio Anil (São Luís, Estado do Maranhão) - DOI: 10.4025/actascibiolsci.v25i2.1978
Provedor de dados: 3
Autores: Serra, Carmen Lucia Martins; UFMA; Cavalcante, Paulo Roberto Saraiva; UFMA; Alves, Lucia Maria Coelho; UFMA; Nascimento, Adenilde Ribeiro; UFMA; Diniz, Silvia Cristina Costa de Santana de; UFMA.
Avaliação dos parâmetros físico-químicos e isolamento de Vibrio parahaemolyticus em águas do estuário do rio Anil (São Luís-MA, Brasil), em bancos naturais de moluscos. Foi utilizada a técnica do Número Mais Provável (NMP) com enriquecimento e subseqüente isolamento em agar tiossulfato-citrato-sacarose-sais-biliares (TCBS). Adicionalmente, foram monitorados “in situ” alguns parâmetros físico-químicos. As determinações para o pH variaram (7,25 a 7,90), O2 (0,20 a 1,2mg/L), T°C (29 a 32°C), S‰ (5 a 28,0 UPS), NH4+ (0,04 a 3,41mg/L), NO2- (0,10 a 1,91mg/L), PO43- (0,03 a 4,88mg/L). Em 56,1% na estação I e 55,4% na estação II, apresentaram positividade, para V. parahaemolyticus, com níveis entre 1,1x10 e &gt; 1,1x105NMP/mL. Os resultados exigem uma atenção...
Palavras-chave: 2.01.00.00-0    Biologia Geral parâmetros físicos e químicos; Vibrio parahaemolyticus; Estuário; Nutrientes; Análises de água 2.01.00.00-0    Biologia Geral.
Ano: 2003
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1978

Raw oysters can be a risk for infections
Provedor de dados: 55
Autores: Vieira,Regine Helena Silva dos Fernandes; Sousa,Oscarina Viana de; Costa,Renata Albuquerque; Theophilo,Grace Nazareth Diogo; Macrae,Andrew; Fonteles Filho,Antonio Adauto; Rodrigues,Dália dos Prazeres.
The aims of this study were to count and identify sucrose positive and negative vibrios isolated from cultivated Crassostrea rhizophorae oysters during their growing cycle. Every month for 12 months, 10 to 18 oysters were collected for study. Collections occurred at the Center for Studies of Coastal Aquaculture (CSCA), which is associated with the Institute of Marine Science, Labomar, located in Euzebio, Ceará, Brazil. Approximately 150 oysters and their intervalvular liquor were studied. Vibrio Standard Plates Counts (SPC) from oyster meat and their intervalvular liquor varied from 25 to 59,000,000 CFU/g. For most of the 12 months of the oysters' life, it was possible to identify Vibrio parahaemolyticus. Vibrio carchariae was identified in four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vibrio spp; Oyster; Public health; Vibrio parahaemolyticus; Vibrio carchariae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100013

Depuration Times of Donax trunculus and Tapes decussatus
Provedor de dados: 58
Autores: Çolakolu,Fatma Arik; Çardak,Mine; Çolakoglu,Serhat; Künili,Ibrahim Ender.
The present study was performed to determine the depuration time and ability of Donax trunculus (Wedge Clam) and Tapes decussatus (Carpet Shell) contaminated with Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium and Vibrio parahaemolyticus. Clams were contaminated with each bacterium at the level of 7.0 - 8.0 Log10 cfu/g. After contamination, clams were analyzed every 3 h in the first 24 h time period and every 6 h until the 72nd hour. During the depuration process of both clams, the level of bacteria decreased quickly to 40% of initial load in the first 12 h. The results of this study indicate that the depuration time of carpet shells for all bacteria is 66 h. The depuration process of the wedge clam was different from the carpet...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clam; Purification; Vibrio parahaemolyticus; Escherichia coli; Salmonella typhimurium.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300034

Microbial contamination of sand from major beaches in Fortaleza, Ceará State, Brazil
Provedor de dados: 58
Autores: Vieira,Regine Helena Silva dos Fernandes; Rodrigues,Dália dos Prazeres; Menezes,Everardo A.; Evangelista,Norma Suely de Santana; Reis,Eliane M.F. dos; Barreto,Leopoldo Melo; Gonçalves,Flávia Araújo.
The presence of faecal contamination and pathogenic microorganisms in samples of dry and wet sand collected from three major beaches in Fortaleza, Ceará State, Brazil: (Praia do Mucuripe, Praia do Futuro and Praia do Caça e Pesca), during the period of May 1999 to January 2000 was evaluated. Praia do Caça e Pesca had the highest incidence of E. coli in dry sand (56%) followed by Praia do Mucuripe (28%) and Praia do Futuro (16%). In wet sand, results were 48%, 28% and 24% for Praia do Caça e Pesca, Praia do Futuro and Praia do Mucuripe, respectively. Only two samples from Praia do Futuro, one from dry sand and another one from wet sand, were positive for Salmonella. V. parahaemolyticus was isolated from four samples from Praia do Caça e Pesca (two from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sand contamination; Yeasts; Faecal coliforms; Vibrio parahaemolyticus; Salmonella.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200001

Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in Natal, Brazil
Provedor de dados: 58
Autores: Melo,Ligia Maria Rodrigues de; Almeida,Dulce; Hofer,Ernesto; Reis,Cristhiane Moura Falavina dos; Theophilo,Grace Nazareth Diogo; Santos,André Felipe das Mercês; Vieira,Regine Helena Silva dos Fernandes.
Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei) collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil) tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50%) presented multiple antibiotic resistance to ampicillin (90%) and amikacin (60%), while two strains (20%) displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vibrio parahaemolyticus; Litopenaeus vannamei; Multiple antibiotic resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400032