Consulta: subjectFacets:"Vibrio penaeicida"
Registros recuperados: 6
Data/hora: 06/06/2024 08:24:17

A relationship between antimicrobial peptide gene expression and capacity of a selected shrimp line to survive a Vibrio infection
Provedor de dados: 5
Autores: De Lorgeril, Julien; Gueguen, Yannick; Goarant, Cyrille; Goyard, Emmanuel; Mugnier, Chantal; Fievet, Julie; Piquemal, D; Bachere, Evelyne.
Understanding of antimicrobial defence mechanisms of penaeid shrimp should help in the design of efficient strategies for the management and disease control in aquaculture. In this study, we have specifically analysed the expression in circulating hemocytes of antimicrobial peptides (AMPs) encoding genes, such as PEN2 and PEN3, ALF, crustin, lysozyme and a putative cysteine-rich peptide. We evidenced a relationship between the level of expression of some AMPs and the successful response of the shrimp, Litopenaeus stylirostris, to circumvent a pathogenic Vibrio penaeicida infection. Additionally, significant differences in some AMP transcript amounts are evidenced between control, non-selected shrimp line and the third generation breeding of shrimp selected...
Tipo: Text
Palavras-chave: Real time PCR; Cysteine rich peptide; Crustin; Anti LPS factor; Lysozyme; Penaeidins; Immune response; Vibrio penaeicida; Penaeid; Decapoda; Crustacean.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4524.pdf

Pathogenicity of Vibrio penaeicida or white shrimp Litopenaeus vannamei: a cysteine protease-like exotoxin as a virulence factor
Provedor de dados: 5
Autores: Aguirre Guzman, G; Ascencio, F; Saulnier, Denis.
The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p &lt; 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD50) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD50 values of 1.68 and 5.61 mu g protein ind.(-1), respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to...
Tipo: Text
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus vannamei; Cysteine protease; Vibrio penaeicida.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3618.pdf

Epidémiologie et facteurs de virulence des bactéries du genre Vibrio responsables de mortalité de crevettes d'élevage en Nouvelle-Calédonie. Pespectives de lutte
Provedor de dados: 5
Autores: Goarant, Cyrille.
La Nouvelle-Calédonie présente de nombreux atouts pour la pénéiculture. Le ralentissement brutal de son développement en 1993 s'explique par l'émergence d'une pathologie saisonnière qui affecte les crevettes juvéniles en grossissement, mettant en cause des bactéries du genre Vibrio et qui a reçu le nom de Syndrome 93. Vibrio penaeicida et V. nigripulchritudo sont deux espèces au pouvoir pathogène particulièrement élevé qui affectent les élevages. Une étude de typage moléculaire a permis de préciser leur épidémiologie et de proposer quelques conseils d'ordre zoosanitaire. Un suivi épidémiologique par amplification génique est en cours pour V. penaeicida, qui montre l'importance des facteurs écologiques dans l'apparition des épisodes pathologiques. La...
Tipo: Text
Palavras-chave: Litopenaeus stylirostris; Vibrio penaeicida; Epidémiologie; Pathogénicité; Nouvelle-Calédonie.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00444/55510/57060.pdf

Proteinaceous exotoxins of shrimp-pathogenic isolates of Vibrio penaeicida and Vibrio nigripulchritudo
Provedor de dados: 5
Autores: Aguirre-guzman, Gabriel; Labreuche, Yannick; Ansquer, Dominique; Espiau, Benoit; Levy, Peva; Ascencio, Felipe; Saulnier, Denis.
The pathogenicity of two V. penaeicida strains, AM101 and KH-1, with different geographic origin, and V. nigripulchritudo strain AM102, were investigated in juvenile blue shrimp species Litopenaeus stylirostris. Alive bacteria and protein fractions (PFs) obtained from cell-free supernatants (CFS) were used in experimental challenges. Strains AM102, AM101, and KH-1 produced respectively 60, 54 and 12% mortality at 96h after infection using 104 cfu mL-1 of bacterial suspension. Exocellular toxin-like factors were evidenced in CFS from the New Caledonian strains (AM102 and AM101) but not the Japanese strain (KH-1). At 48h post injection of each CFS, mortality rates were respectively 96,98 and 5% when these strains were cultivated at 20°C, whereas only O,16...
Tipo: Text
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus stylirostris; Vibrio nigripulchritudo; Vibrio penaeicida.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2807.pdf

Rapid and sensitive PCR detection of Vibrio penaeicida, the putative etiological agent of syndrome 93 in New Caledonia
Provedor de dados: 5
Autores: Saulnier, Denis; Avarre, Jean-christophe; Le Moullac, Gilles; Ansquer, Dominique; Levy, Peva; Vonau, Vincent.
Experimental infections of Penaeus (Litopenaeus) stylirostris were performed with a Vibrio penaeicida strain (AM101) isolated in New Caledonia from Syndrome 93 diseased shrimp. Cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that V. penaeicida could be the etiological agent of Syndrome 93. The median lethal dose (LD50) for AM101 was 1.3 x 104 CFU (colony forming units) ml-1 by immersion and less than 5 CFU shrimp-1 by intramuscular challenge, with mortality outbreaks at 48 and 22 h after challenge, respectively. A polymerase chain reaction (PCR) detection assay using a primer set designed from the 16S ribosomal RNA gene of V....
Tipo: Text
Palavras-chave: Small subunit rDNA gene; Penaeus (Litopenaeus) stylirostris; Vibriosis; Shrimp disease; PCR diagnosis; Vibrio penaeicida; Syndrome 93.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-4436.pdf

Toxic factors of Vibrio strains pathogenic to shrimp
Provedor de dados: 5
Autores: Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B.
Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida...
Tipo: Text
Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf