Consulta: subjectFacets:"Vibrio tapetis"
Registros recuperados: 11
Data/hora: 11/06/2024 03:17:08

Relation between Biofilm and Virulence in Vibrio tapetis: A Transcriptomic Study
Provedor de dados: 5
Autores: Rodrigues, Sophie; Paillard, Christine; Van Dillen, Sabine; Tahrioui, Ali; Berjeaud, Jean-marc; Dufour, Alain; Bazire, Alexis.
Marine pathogenic bacteria are able to form biofilms on many surfaces, such as mollusc shells, and they can wait for the appropriate opportunity to induce their virulence. Vibrio tapetis can develop such biofilms on the inner surface of shells of the Ruditapes philippinarum clam, leading to the formation of a brown conchiolin deposit in the form of a ring, hence the name of the disease: Brown Ring Disease. The virulence of V. tapetis is presumed to be related to its capacity to form biofilms, but the link has never been clearly established at the physiological or genetic level. In the present study, we used RNA-seq analysis to identify biofilm- and virulence-related genes displaying altered expression in biofilms compared to the planktonic condition. A...
Tipo: Text
Palavras-chave: Biofilm; Virulence; Vibrio tapetis; Transcriptome; Quorum sensing; Type VI secretion system; Brown ring disease.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00470/58140/60611.pdf

External pH modulation during the growth of Vibrio tapetis, the aetiological agent of brown ring disease
Provedor de dados: 5
Autores: Rahmani, Alexandra; Mathien, Clémentine; Bidault, Adeline; Le Goïc, Nelly; Paillard, Christine; Pichereau, Vianney.
Aims Brown Ring Disease (BRD) is an infection of the Manila clam Ruditapes philippinarum due to the pathogen Vibrio tapetis. During BRD, clams are facing immunodepression and shell biomineralization alteration. In this paper, we studied the role of pH on the growth of the pathogen and formulated hypothesis on the establishment of BRD by V. tapetis. Methods and Results In this study, we monitored the evolution of pH during the growth of V. tapetis in a range of pH and temperatures. We also measured the pH of Manila clam hemolymph and extrapallial fluids during infection by V. tapetis. We highlighted that V. tapetis modulates the external pH during its growth, to a value of 7.70. During the development of BRD, V. tapetis also influences extrapallial fluids...
Tipo: Text
Palavras-chave: BRD; Modulation effect; Neutralizing activity; PH; PH modifications; Ruditapes philippinarum; Vibrio tapetis.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00629/74142/73669.pdf

Development of a Taqman real-time PCR assay for rapid detection and quantification of Vibrio tapetis in extrapallial fluids of clams
Provedor de dados: 5
Autores: Bidault, Adeline; Richard, Gaelle G.; Le Bris, Cedric; Paillard, Christine.
The Gram-negative bacterium Vibrio tapetis is known as the causative agent of Brown Ring Disease (BRD) in the Manila clam Venerupis (=Ruditapes) philippinarum. This bivalve is the second most important species produced in aquaculture and has a high commercial value. In spite of the development of several molecular methods, no survey has been yet achieved to rapidly quantify the bacterium in the clam. In this study, we developed a Taqman real-time PCR assay targeting virB4 gene for accurate and quantitative identification of V. tapetis strains pathogenic to clams. Sensitivity and reproducibility of the method were assessed using either filtered sea water or extrapallial fluids of clam injected with the CECT4600(T) V. tapetis strain. Quantification curves of...
Tipo: Text
Palavras-chave: Vibrio tapetis; VirB4 gene; Taqman real-time PCR; Molecular diagnostic; Venerupis philippinarum; Marine pathogen; Brown ring disease.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00308/41909/73194.pdf

Optimisation du diagnostic et épidémiologie de la maladie de l'anneau brun sur la côte Atlantique
Provedor de dados: 5
Autores: Beguier, Jeremy.
A l'échelle nationale, la conchyliculture a connu des épizooties catastrophiques ; l'huître portugaise (Crassostrea angulata) a disparu des côtes françaises, suite à des infections impliquant des iridovirus. L'huître plate (Ostrea edulis) a vus sa production fortement réduite suite à l'introduction de deux maladies parasitaires, la bonamiose et la marteiliose. La maladie de l'anneau brun, maladie d'origine bactérienne due à Vibrio tapetis a engendré depuis 1987 en Europe des mortalité allant de 60 à 80% dans les élevages, écloseries et bancs naturels de palourdes japonaises Ruditapes philippinarum et de palourdes européennes R. decussatus, compromettant le développement de cette filière aquacole. Cette étude a pour but d'obtenir une image de la maladie de...
Tipo: Text
Palavras-chave: Palourdes; Ruditapes philippinarum; Ruditapes decussatus; Anneau brun; Vibrio tapetis; Biologie moléculaire.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00034/14480/11780.pdf

A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum
Provedor de dados: 5
Autores: Smits, M.; Artigaud, Sebastien; Bernay, B.; Pichereau, Vianney; Bargelloni, L.; Paillard, Christine.
Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by...
Tipo: Text
Palavras-chave: Bivalve; Diseases; Resistance; Manila clam; Vibrio tapetis; Proteomics; Immune response.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00615/72670/71758.pdf

Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection
Provedor de dados: 5
Autores: Le Bris, Cedric; Richard, Gaelle; Paillard, Christine; Lambert, Christophe; Seguineau, Catherine; Gauthier, Olivier; Pernet, Fabrice; Guerard, Fabienne.
Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection....
Tipo: Text
Palavras-chave: Venerupis philippinarum; Vibrio tapetis; Immunity; Phenoloxidase; Superoxide dismutase.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00221/33257/31733.pdf

Implication of the Type IV Secretion System in the Pathogenicity of Vibrio tapetis, the Etiological Agent of Brown Ring Disease Affecting the Manila Clam Ruditapes philippinarum
Provedor de dados: 5
Autores: Rahmani, Alexandra; Delavat, François; Lambert, Christophe; Le Goic, Nelly; Dabas, Eric; Paillard, Christine; Pichereau, Vianney.
Vibrio tapetis is a Gram-negative bacterium that causes infections of mollusk bivalves and fish. The Brown Ring Disease (BRD) is an infection caused by V. tapetis that primarily affects the Manila clam Ruditapes philippinarum. Recent studies have shown that a type IV secretion system (T4SS) gene cluster is exclusively found in strains of V. tapetis pathogenic to clams. However, whether the T4SS is implicated or not during the infection process remains unknown. The aim of this study was to create and characterize a V. tapetis T4SS null mutant, obtained by a near-complete deletion of the virB4 gene, in order to determine the role of T4SS in the development of BRD. This study demonstrated that the T4SS is neither responsible for the loss of hemocyte adhesion...
Tipo: Text
Palavras-chave: Brown Ring Disease; Vibrio tapetis; Ruditapes philippinarum; Hemocytes; Type four secretion system; Rounding phenotype; Colonization; Mutagenesis.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00692/80383/83507.pdf

Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis
Provedor de dados: 5
Autores: Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C.
Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity...
Tipo: Text
Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf

Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs
Provedor de dados: 5
Autores: Dias, Graciela M.; Bidault, Adeline; Le Chevalier, Patrick; Choquet, Gwenaelle; Sarkissian, Clio Der; Orlando, Ludovic; Medigue, Claudine; Barbe, Valerie; Mangenot, Sophie; Thompson, Cristiane C.; Thompson, Fabiano L.; Jacq, Annick; Pichereau, Vianney; Paillard, Christine.
The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam Venerupis philippinarum introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, Vibrio tapetis, which is also pathogenic to fish. Here we report the first assembly of the complete genome of V. tapetis CECT4600T, together with the genome sequences of 16 additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan-and core genomes and to identify putative virulence factors. The V. tapetis core genome consists of 3,352 genes, including multiple potential virulence factors represented by haemolysins, transcriptional regulators, Type I...
Tipo: Text
Palavras-chave: Vibrio tapetis; Venerupis philippinarum; Comparative genomics; Pathogenicity; T4SS; Pangenome; Core genome.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00429/54077/72091.pdf

Vibrio tapetis, the Causative Agent of Brown Ring Disease, Forms Biofilms with Spherical Components
Provedor de dados: 5
Autores: Rodrigues, Sophie; Paillard, Christine; Le Pennec, Gael; Dufour, Alain; Bazire, Alexis.
Vibrio tapetis is a marine bacterium causing Brown Ring Disease (BRD) in the Manila clam Ruditapes philippinarum. V. tapetis biofilm formation remains unexplored depite the fact that it might be linked to pathogenicity. Our objectives were to characterize the in vitro biofilm formation of V tapetis and evaluate the effects of culture conditions. Biofilm structure and its matrix composition were examined by confocal laser scanning microscopy and scanning electron microscopy. V tapetis was able to form biofilms on a glass substratum within 24 h. Polysaccharides and extracellular DNA of the biofilm matrixes were differently distributed depending on the V tapetis strains. Spherical components of about 1-2 mu m diameter were found at the biofilm surface. They...
Tipo: Text
Palavras-chave: Vibrio tapetis; Biofilm; Brown Ring Disease; Ruditapes philippinarum; Spherical components; Temperature effect; Salinity effect.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00307/41783/42796.pdf

Metabolic responses of clam Ruditapes philippinarum exposed to its pathogen Vibrio tapetis in relation to diet
Provedor de dados: 5
Autores: Richard, Gaelle; Guerard, Fabienne; Corporeau, Charlotte; Lambert, Christophe; Paillard, Christine; Pernet, Fabrice.
We investigated the effect of brown ring disease (BRD)1 development and algal diet on energy reserves and activity of enzymes related to energy metabolism, antioxidant system and immunity in Manila clam, Ruditapes philippinarum. We found that algal diet did not impact the metabolic response of clams exposed to Vibrio tapetis. At two days post-injection (dpi), activities of superoxide dismutase and glutathione peroxidase (GPx) decreased whereas activities of nitric oxide synthase (iNOS) and catalase increased in infected clams, although no clinical signs were visible (BRD-). At 7 dpi, activities of several antioxidant and immune-related enzymes were markedly increased in BRD-likely indicating an efficient reactive oxygen species (ROS) scavenging compared to...
Tipo: Text
Palavras-chave: Antioxidant enzymes; Immunity; Energetic metabolism; Ruditapes philippinarum; Vibrio tapetis.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00316/42684/42057.pdf