Consulta: subjectFacets:"Vibriosis"
Registros recuperados: 26
Data/hora: 06/06/2024 08:46:35

DEDUCTION : A research project for shrimp farming sustainability in New-Caledonia
Provedor de dados: 5
Autores: Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly.
New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government...
Tipo: Text
Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf

Molecular epidemiology of Vibrio nigripulchritudo, a pathogen of cultured penaeid shrimp (Litopenaeus stylirostris) in New Caledonia
Provedor de dados: 5
Autores: Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Saulnier, Denis; Leroux, Frederique.
A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into this possibly emerging pathogen. Additionally, results obtained with the two molecular typing techniques were congruent and allowed discriminating the strains associated with the "Summer Syndrome" from strains isolated from other contexts, especially the other shrimp...
Tipo: Text
Palavras-chave: Virulence; Epidemiology; Typing; MLST; AP PCR; Mariculture; Vibriosis.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1913.pdf

Identification de marqueurs génétiques de la virulence chez Vibrio nigripulchritudo, un pathogène de crevettes pénéides en Nouvelle-Calédonie
Provedor de dados: 5
Autores: Reynaud, Yann.
Since 1997, a new pathology seasonally occurs in new caledonian shrimp farms during the warm season and was named Summer Syndrome. Diseased Litopenaeus stylirostris shrimp suffer from a septicemic vibriosis which was attributed to V. nigripulchritudo. Preliminary studies based on a collection of V. nigripulchritudo strains have brought to light different virulence levels according to experimental infections results; three virulence statuses were defined: highly (HP), moderately (MP) and non pathogenic (NP). The aim of this work was to genetically characterize virulent V. nigripulchritudo strains. In a first step the genetic diversity of 58 V. nigripulchritudo strains was analyzed by MLST and AP-PCR, revealing a cluster of HP and MP strains, characterized...
Tipo: Text
Palavras-chave: Plasmide; SSH; Épidémiology; Virulence; Shrimp; Vibriosis; Vibrio nigripulchritudo; Plasmide; SSH; Épidémiologie; Virulence; Crevette; Vibriose; Vibrio nigripulchritudo.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-3906.pdf

Nutrient and microbial dynamics in eutrophying shrimp ponds affected or unaffected by vibriosis
Provedor de dados: 5
Autores: Lemonnier, Hugues; Courties, Claude; Mugnier, Chantal; Torreton, Jean-pascal; Herbland, Alain.
A field survey was conducted on two intensive shrimp farms using similar technical practices: one (DF) historically affected by a vibriosis, the other (HC) in which the pathogen has been observed although no mortality event has occurred. Because historical data suggest that eutrophication process may directly or indirectly play a role in the disease outbreak, we focussed our research on its dynamics. A higher variability of the phytoplanktonic compartment linked to an imbalance in the molar N:P ratio was observed in farm DF compared to farm HC, implying a modification on the linkage between the bacteria and phytoplankton compartments at DF. The beginning of the mortality outbreak at DF followed a shift from picoto nanophytoplankton. The organic matter...
Tipo: Text
Palavras-chave: Aquaculture; Shrimp; Eutrophication; Ecosystem shift; Phytoplankton; Vibriosis.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11368/8119.pdf

Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris
Provedor de dados: 5
Autores: Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
in this study we evaluated the effects of the probiotic Pediococcus acidilactici MA18/5M on shrimp, Litopenaeus stylirostris (also called Penaeus stylirostris), first on antioxidant defences and secondly on the oxidative stress status in the shrimps' haemolymph and digestive gland. We conducted two experiments with the same protocol in which shrimps were fed two diets for three weeks: a control diet and a probiotic diet containing 1 g of live P. acidilactici MA18/5M kg(-1). In the first experiment, the shrimps were found to be healthy over the trial period; no mortalities and non-detectable signs of infection were recorded. These resulted in high final survival rates (above 90% in both treatments). On the other hand, during the second trial, carried out at...
Tipo: Text
Palavras-chave: Vibriosis; Antioxidant defences; Oxidative stress; Probiotic; Shrimps.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6780.pdf

Vibriosis in bivalves : review of recent molecular, biochemical, and physiological studies
Provedor de dados: 5
Autores: Paillard, Christine; Nicolas, Jean-louis; Le Chevalier, Patrick; Lambert, Christophe; Le Boulay, Christine; Berthe, Franck; Haras, Dominique.
The emergence of new vibriosis in the European Atlantic coasts has been associated with mass mortalities of molluscs causing important economic losses. For examples, Vibrio carchariae, a bacteria already isolated from shark, has been associated with a severe Haliotis epizooty in hatchery. In oysters, Crass()strea gigas, a strain closely related to Vibrio splendidus is suspected to be associated with summer mortalities.
Tipo: Text
Palavras-chave: Pathogenic vibrios; Bivales; Vibriosis; Bacteriology.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3287.pdf

Bacterial disease in marine bivalves, a review of recent studies: Trends and evolution
Provedor de dados: 5
Autores: Paillard, Christine; Leroux, Frederique; Borrego, Juan.
The main microbial diseases affecting marine cultured bivalves have been revised on the basis of the etiologic agents, pathogenesis and pathogenicity. Several recent bivalve-interaction models have been studied, including Pecten larvae-Vibrio pectinicida, brown ring disease, juvenile oyster disease, Pacific oyster nocardiosis and summer mortalities of oysters. In addition, the taxonomy and phylogeny of new potential bivalve pathogens and their virulence factors have been established. Facing the difficulty of identifying bacterial strains associated with molluscan diseases (mainly vibriosis), polyphasic approaches have been developed to correlate the phenotype and genotype of potential pathogens. By evaluating likely virulence mechanisms, developing...
Tipo: Text
Palavras-chave: Diagnostic tools; Virulence factors; Pathogenicity; Pathogenesis; Etiology; Bivalve mollusc; Vibriosis.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-407.pdf

Two Vibrio splendidus related strains collaborate to kill Crassostrea gigas: taxonomy and host alterations
Provedor de dados: 5
Autores: Gay, Melanie; Renault, Tristan; Pons, Anne-marie; Le Roux, Frédérique.
For several years, strains phenotypically related to Vibrio splendidus have been associated with mortality outbreaks of molluscs. A former study on Crassostrea gigas demonstrated the genetic diversity of V splendidus strains associated with diseased animals. Another study suggested that different strains may act in an additive/synergistic way leading to higher C. gigas mortality rates. Here, a strain pair (31+32) was characterised at taxonomic and virulence levels. Using a polyphasic approach, these strains were confirmed to be V splendidus-related, without a clear discrimination between V. kanaloae and V. pomeroyi since hybridisation rates with both these strains were above 70%. Following experimental infection of C. gigas by injection in the adductor...
Tipo: Text
Palavras-chave: Histopathology; Molecular taxonomy; Agonism; Vibriosis.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2912.pdf

Scallop larval survival from erythromycin treated broodstock after conditioning without sediment
Provedor de dados: 5
Autores: Holbach, Marine; Robert, Rene; Boudry, Pierre; Petton, Bruno; Archambault, Philippe; Tremblay, Rejean.
Pathogenic bacteria are known to be one of the main factors affecting Pecten maximus larval survival in hatcheries. As a result, juvenile production often relies on the use of antibiotics during larval culture. However, limitations of the usage of chemicals such as chloramphenicol in aquaculture have been reinforced due to their negative environmental impact and alternatives are accordingly needed. Thus, the importance of bacterial transfer from oocytes to larvae has been studied here as well as procedures to limit larval mortality in P. maximus. In order to reduce bacterial contamination during larval development, we focused on two periods, broodstock conditioning and post-fertilization. The animals were conditioned for 2 months with two erythromycin...
Tipo: Text
Palavras-chave: Scallop; Broodstock conditioning; Larval survival; Vibriosis.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00240/35171/33675.pdf

Diagnosis of vibriosis in the era of genomics: lessons from invertebrates
Provedor de dados: 5
Autores: Le Roux, Frederique.
Global changes linked to increases in temperature and ocean acidification, but also to more direct anthropogenic influences such as aquaculture, have caused a worldwide increase in the reports of Vibrio-associated illnesses affecting humans and also animals such as shrimp and molluscs. Investigation of the emergence of Vibrio pathogenesis events requires the analysis of microbial evolution at the gene, genome and population levels, in order to identify genomic modifications linked to increased virulence, resistance and/or prevalence, or to recent host shift. From a more applied point of view, the elucidation of virulence mechanisms is a prerequisite to devising prophylactic methods to fight infectious agents. In comparison with human pathogens, fairly...
Tipo: Text
Palavras-chave: Écologie; Émergence; Évolution; Invertébrés marins; Vibriose; Virulence; Ecology; Emergence; Evolution; Marine invertebrate; Vibriosis; Virulence.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00344/45560/46722.pdf

Probiotic P-acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia
Provedor de dados: 5
Autores: Castex, Mathieu; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Wabete, Nelly; Nicolas, Jean-louis; Schmidely, P; Mariojouls, C.
We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing probiotic-coated pellets against control was replicated in two earthen ponds. High mortalities, characteristic of Summer syndrome, were observed during the first 2 weeks of the trial. The probiotic improved production in the treated cages from both ponds with increases...
Tipo: Text
Palavras-chave: Vibriosis; Floating cages; Litopenaeus stylirostris; Pediococcus acidilactici; Probiotic.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4079.pdf

Interaction de la bactérie Vibrio harveyi avec son hôte, l'hormeau Haliotis tuberculata : approches physiologiques, cellulaires et moléculaires
Provedor de dados: 5
Autores: Travers, Marie-agnes.
Abalone aquaculture only recently developed in western Europe, but already it has to face numerous pathologies. Since 1998, the natural and farm stocks of Haliotis tuberculata suffer regularly high mortalities, mainly due to the pathogenic bacterium, Vibrio harveyi. The vibriosis of the European abalone, in particular its macroscopic signs, and the conditions necessary for its development have been studied in detail by field and laboratory experiments. We have been able to show the importance of the interplay between water temperature (&gt;17°C), animal physiology (reproduction period) and a pathogenic strain (harbouring the plasmid pVCR1) for the development of this abalone disease. A limited localisation study of the abalone target tissues (gills, muscle...
Tipo: Text
Palavras-chave: Ormeau; Haliotis tuberculata; Bactéries; Vibrio harveyi; Vibriose; Température; Immunité; Reproduction; Hémocytes; MAP Kinases; Plasmide; Virulence; GFP; Abalone; Haliotis tuberculata; Bacteria; Vibrio harveyi; Vibriosis; Temperature; Immunity; Reproduction; Haemocytes; MAP Kinases; Plasmid; Virulence; GFP.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00176/28729/27196.pdf

Report about mollusc diseaes
Provedor de dados: 5
Autores: Berthe, Franck.
Of the 75 laboratories contacted through the survey, 54 answered, 14 of which are partially or totally devoted to mollusc diseases. In fact, only 4 laboratories are devoted full-time to this work. Of the 14 laboratories that declared to work on the diagnosis of mollusc diseases, 12 stated that they worked with mussels, 12 with oysters, 9 with clams and 8 with other mollusc species. This is coherent with available production information. According to production data in 2001 (FAO source), the main mollusc species in the region are the blue mussel, Mytilus edulis (297,485 tonnes), the Mediterranean mussel, Mytilus galloprovincialis (131,014 tonnes), the Pacific cupped oyster, Crassostrea gigas (127,323 tonnes, an important part of which is produced on the...
Tipo: Text
Palavras-chave: Bonamia existiosus; Bonamis ostreae; Bonamiosis; Marteilia sydneyi; Marteilia maurini; Marteilia refringens; Marteiliosis; Perkinsus marinus; Perkinsus olseni; Perkinsus atlanticus; Perkinsosis; Vibriosis; Herpes like virus; Oysters; Pathology; Mollusc diseases.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-3300.pdf

Dynamics of phytoplankton communities in eutrophying tropical shrimp ponds affected by vibriosis
Provedor de dados: 5
Autores: Lemonnier, Hugues; Lantoine, Francois; Courties, Claude; Guillebault, Delphine; Nezan, Elisabeth; Chomerat, Nicolas; Escoubeyrou, Karine; Galinie, Christian; Blockmans, Bernard; Laugier, Thierry.
Tropical shrimp aquaculture systems in New Caledonia regularly face major crises resulting from outbreaks of Vibrio infections. Ponds are highly dynamic and challenging environments and display a wide range of trophic conditions. In farms affected by vibriosis, phytoplankton biomass and composition are highly variable. These conditions may promote the development of harmful algae increasing shrimp susceptibility to bacterial infections. Phytoplankton compartment before and during mortality outbreaks was monitored at a shrimp farm that has been regularly and highly impacted by these diseases. Combining information from flow cytometry, microscopy, pigment and phylogenetic analysis, the presence of Picocyanobacteria, Prasinophyceae and Diatomophyceae were...
Tipo: Text
Palavras-chave: Tropical aquaculture pond; Litopenaeus stylirostris; Vibriosis; Eutrophication; Phytoplankton; Harmful algae.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00343/45411/44986.pdf

Biological, physiological, immunological and nutritional assessment of farm-reared Litopenaeus stylirostris shrimp affected or unaffected by vibriosis
Provedor de dados: 5
Autores: Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene.
Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical...
Tipo: Text
Palavras-chave: Vibriosis; Penaeid shrimp; Field survey; Physiology; Immunology; Nutritional status.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00176/28764/27567.pdf

Rapid and sensitive PCR detection of Vibrio penaeicida, the putative etiological agent of syndrome 93 in New Caledonia
Provedor de dados: 5
Autores: Saulnier, Denis; Avarre, Jean-christophe; Le Moullac, Gilles; Ansquer, Dominique; Levy, Peva; Vonau, Vincent.
Experimental infections of Penaeus (Litopenaeus) stylirostris were performed with a Vibrio penaeicida strain (AM101) isolated in New Caledonia from Syndrome 93 diseased shrimp. Cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that V. penaeicida could be the etiological agent of Syndrome 93. The median lethal dose (LD50) for AM101 was 1.3 x 104 CFU (colony forming units) ml-1 by immersion and less than 5 CFU shrimp-1 by intramuscular challenge, with mortality outbreaks at 48 and 22 h after challenge, respectively. A polymerase chain reaction (PCR) detection assay using a primer set designed from the 16S ribosomal RNA gene of V....
Tipo: Text
Palavras-chave: Small subunit rDNA gene; Penaeus (Litopenaeus) stylirostris; Vibriosis; Shrimp disease; PCR diagnosis; Vibrio penaeicida; Syndrome 93.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-4436.pdf

Effet des conditions environnementales sur le développement des pathologies à Vibrio dans les élevages de crevettes en Nouvelle-Calédonie
Provedor de dados: 5
Autores: Lemonnier, Hugues.
Shrimp aquaculture in New Caledonia is a developing industry, exclusively based on the species Litopenaeus stylirostris. However, it is subject to mortalities during grow out as observed all over the world. Since 1993, shrimp reared during the cold period are affected by a disease named "syndrome 93". As a consequence, the whole industry has become seasonal and produces essentially during the warm season. The climatic conditions during the periods of transition between the two main seasons could be responsible for an environmental stress and the origin reason for mortality outbreaks. Stocking density is the main risk factor. It increases the probability of the appearance and development of the epizooty in the pond. The "Summer Syndrome", a septicemic...
Tipo: Text
Palavras-chave: New Caledonia; Risk factors; Environmental stress; Vibriosis; Epizooty; Pond management; Eutrophication; Pond health; Shrimp; Litopenaeus stylirostris; Peneids; Aquaculture; Nouvelle  Calédonie; Facteur de risque; Stress environnemental; Vibrioses; Pratiques d'élevage; Eutrophisation; Santé de l'écosystème bassin; Crevette; Litopenaeus stylirostris; Pénéides; Aquacultre.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/these-2643.pdf

"Summer syndrome" in Litopenaeus stylirostris grow out ponds in New Caledonia: zootechnical and environmental factors
Provedor de dados: 5
Autores: Lemonnier, Hugues; Herbland, Alain; Salery, Lucas; Soulard, Benoit.
The "Summer Syndrome", a septicemic vibriosis caused by Vibrio nigripulchritudo, is responsible for one of the two main seasonal mortalities which affect shrimp aquaculture in New Caledonia. It was identified for the first time in December 1997 in an intensive shrimp farm (called farm DF) and has been enzootic ever since. The "Summer syndrome" affects shrimp during warm-season growout. Although the geographic area concerned is limited, it is a potential threat for the industry in that the disease could spread. Analysis of grow out data from 1991 to 2002 in this farm was carried out in order to characterize the disease and the environmental conditions in relation to mortality. Results of farm DF were compared to data from farms not affected by the disease...
Tipo: Text
Palavras-chave: Eutrophication; Pond management; Vibriosis; Shrimp culture ponds.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1868.pdf

Potential environmental drivers of a regional blue mussel mass mortality event (winter 2014, Breton Sound, France)
Provedor de dados: 5
Autores: Polsenaere, Pierre; Soletchnik, Patrick; Le Moine, Olivier; Gohin, Francis; Robert, Stephane; Pepin, Jean-francois; Stanisiere, Jean-yves; Dumas, Franck; Bechemin, Christian; Goulletquer, Philippe.
In the context of global change, increasing mariculture production has raised particular concerns regarding its environmental impact and sustainability. Molluscs and particularly blue mussel account for a significant part of this total production. Although blue mussels are considered as pretty resilient to environmental disturbances, we report in this study an unprecedented mussel mortality event that occurred during the winter 2014 in the Breton Sound. 9000 metric tonnes of mussels were lost and mortality rates up to 100% were recorded at some farming areas. Through a coupling approach, the present work aimed to better understand the potential environmental drivers associated to those mortalities. Firstly we analysed long-term in situ and satellite data...
Tipo: Text
Palavras-chave: Mytilus edulis mortalities; Climate change; Breton sound; Long-term monitoring; Hydrodynamic modelling; Vibriosis.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00376/48708/49083.pdf

Quantification of Vibrio penaeicida, the etiological agent of Syndrome 93 in New Caledonian shrimp, by real-time PCR using SYBR Green I chemistry
Provedor de dados: 5
Autores: Goarant, Cyrille; Merien, F.
Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular...
Tipo: Text
Palavras-chave: Vibriosis; Vibrio; Real time PCR; Quantification; Mariculture; Extraction techniques.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1903.pdf

Effect du Vibrio P1, pathogene de Ruditapes philippinarum , sur d'autres especes de bivalves
Provedor de dados: 5
Autores: Maes, P; Paillard, C.
The symptom which characterizes the brown ring disease (BRD) in Manilla clam Ruditapes philippinarum has been observed in other Bivalvia species (Veneridae and Pectinidae). Vibrio P1, responsible of the BRD in R. philippinarum , has been detected in other Veneridae which were carrying the symptom. In mussel and oysters which are cultivated in Europe, the symptom has never been reported and Vibrio P1 has not been detected. Experimental contaminations of bivalves of commercial interest with the pathogen showed a clear Vibrio P1 sensitivity of European clam R. decussatus ; this latter seems however to be less sensitive than the Manilla clam.
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Bivalvia; Diseases; Marine molluscs; Pathology; Vibriosis.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-926.pdf

Evaluation du probiotique bactérien Pediococcus acidilactici MA18/5M chez la crevette pénéide Litopenaeus stylirostris en Nouvelle- Calédonie
Provedor de dados: 5
Autores: Castex, Mathieu.
P. acidilactici MA 18/5M is a dietary probiotic (BACTOCELL®) which has shown its efficiency on several terrestrial and aquatic species. The present thesis aimed to investigate more fully the effects of this probiotic on cultured penaeid shrimps. For this study, we chose a global approach by replacing the problem studied in a model of interactions between three compartments: the shrimp, its intestinal microflora and the probiotic. Having first defined how the probiotic should be administrated, we studied its effects under controlled laboratory conditions at several levels of Litopenaeus stylirostris biology: (i) Nutritional status and growth, (ii) associated bacterial microflora, and (iii) health status with respect to oxidative stress. Then the probiotic...
Tipo: Text
Palavras-chave: Floating cages; Vibriosis; Oxidative stress; Intestinal microflora; Nutrition; Growth; Shrimp; Pediococcus acidilactici; Probiotic; Cages flottantes; Vibriose; Stress oxydant; Microflore intestinale; Nutrition; Croissance; Crevette; Pediococcus acidilactici; Probiotique.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/these-6814.pdf

Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum
Provedor de dados: 5
Autores: Paillard, Christine; Gausson, Sabrina; Nicolas, Jean-louis; Le Pennec, Jean Paul; Haras, Dominique.
Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V...
Tipo: Text
Palavras-chave: DOT BLOT hybridisation; SSP PCR; Vibriosis; Bivalve; Detection; Diagnostic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1692.pdf

Cross breeding of different domesticated lines as a simple way for genetic improvement in small aquaculture industries: Heterosis and inbreeding effects on growth and survival rates of the Pacific blue shrimp Penaeus (Litopenaeus) stylirostris
Provedor de dados: 5
Autores: Goyard, Emmanuel; Goarant, Cyrille; Ansquer, Dominique; Brun, Pierre; De Decker, Sophie; Dufour, Robert; Galinie, C; Peignon, Jean-marie; Pham, Dominique; Vourey, Elodie; Harache, Yves; Patrois, Jacques.
Two populations of the Latin American shrimp Penaeus (Litopenaeus)stylirostris domesticated in Hawaii and in New Caledonia were previously shown to be genetically differentiated and proven highly inbred. In New Caledonia, where different Vibriosis affect shrimp production and antibiotic use is banned in growing ponds, the Hawaiian population was introduced to increase the allelic variability available for local shrimp farmers and start a genetic improvement program. Growth and survival rates of the two pure populations and the two-way F-1-hybrids obtained by breeding Hawaiian animals with New Caledonian animals were assessed in several simple experiments (earthen ponds, floating cages and experimental infection challenges) during two years on two...
Tipo: Text
Palavras-chave: Vibriosis; Survival; Growth; Penaeus Litopenaeus stylirostris; Shrimp; Cross breeding; Genetic improvement.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4316.pdf

Microbioal assessment of el-max farm
Provedor de dados: 20
Autores: Elsersy, N.A.; Abo-Ella, G.M.; El-Shenawy, M.; Abdel-Mawla, E.
El-Max fish farm is one of the most important natural fish farm in Alexandria which is suffering from die-off of large amounts of fish. Facing this problem we must deal with the estimation of the quality of the farm raring unit environment (ponds). Analysis of samples using the Bray - Curtis similarity coefficient test yielded that, the input and El-Khandac were similar at (97%), while the eight ponds were classified at different level of similarity as follows: pond1 (B1) and pond7 (B7) were similar at (86%), B2 and B3 were similar at (90%), B14, B10 and B9 were similar at (93%), where as B11 is singly separated at (83%). Moreover, results indicate that distribution of the different bacterial groups was affected with one or more chemical parameters such as...
Tipo: Journal Contribution
Palavras-chave: Vibriosis; Fish farm; E. coli; Streptococcus faecalis Microbiological analysis Microbiology Fish farms Microbiological analysis Microbiology http://aims.fao.org/aos/agrovoc/c_25302 http://aims.fao.org/aos/agrovoc/c_4800.
Ano: 2005
URL: http://hdl.handle.net/1834/1262

Probiotic actions of Bacillus cereus var. toyoi and Saccharomyces boulardii in silver catfish (Rhamdia quelen) larvae culture
Provedor de dados: 96
Autores: Souza,Diego Moreira de; Martins,Gabriel Bernardes; Piedras,Sérgio Renato Noguez; Pouey,Juvêncio Luís Osório Fernandes; Robaldo,Ricardo Berteaux; Leite,Fabio Pereira Leivas.
The objective of this study was to evaluate the use of Bacillus cereus var. toyoi and Saccharomyces boulardii as probiotics to improve Rhamdia quelen culture. Six hundred larvaes (0.16±0.07 g) were divided in three replicate tanks (25-L recirculation, 20 ºC, photoperiod of 12 h light/12 h darkness) per treatment and were randomly assigned to the following treatments: Bacillus cereus var. toyoi; Saccharomyces boulardii; B. toyoi and S. boulardii; and control (without probiotic addition) for a period of 30 days. The fish were fed five times daily (56% crude protein - Supra alevino inicial®) and the probiotics were applied in water once a day. The doses of probiotics were &lt;img src="/img/revistas/rbz/v41n3/aproximadamente.jpg"&gt;5 × 10(8) and &lt;img...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; Feed additives; Silver catfish; Vibriosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000300048