Consulta: subjectFacets:"Virulence factors"
Registros recuperados: 29
Data/hora: 05/06/2024 09:18:59

Bacterial disease in marine bivalves, a review of recent studies: Trends and evolution
Provedor de dados: 5
Autores: Paillard, Christine; Leroux, Frederique; Borrego, Juan.
The main microbial diseases affecting marine cultured bivalves have been revised on the basis of the etiologic agents, pathogenesis and pathogenicity. Several recent bivalve-interaction models have been studied, including Pecten larvae-Vibrio pectinicida, brown ring disease, juvenile oyster disease, Pacific oyster nocardiosis and summer mortalities of oysters. In addition, the taxonomy and phylogeny of new potential bivalve pathogens and their virulence factors have been established. Facing the difficulty of identifying bacterial strains associated with molluscan diseases (mainly vibriosis), polyphasic approaches have been developed to correlate the phenotype and genotype of potential pathogens. By evaluating likely virulence mechanisms, developing...
Tipo: Text
Palavras-chave: Diagnostic tools; Virulence factors; Pathogenicity; Pathogenesis; Etiology; Bivalve mollusc; Vibriosis.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-407.pdf

The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence Mechanisms
Provedor de dados: 5
Autores: Perez-pascual, David; Lunazzi, Aurelie; Magdelenat, Ghislaine; Rouy, Zoe; Roulet, Alain; Lopez-roques, Celine; Larocque, Robert; Barbeyron, Tristan; Gobet, Angelique; Michel, Gurvan; Bernardet, Jean-francois; Duchaud, Eric.
Tenacibaculum maritimum is a devastating bacterial pathogen of wild and farmed marine fish with a broad host range and a worldwide distribution. We report here the complete genome sequence of the T. maritimum type strain NCIMB 2154(T). The genome consists of a 3,435,971-base pair circular chromosome with 2,866 predicted protein-coding genes. Genes encoding the biosynthesis of exopolysaccharides, the type IX secretion system, iron uptake systems, adhesins, hemolysins, proteases, and glycoside hydrolases were identified. They are likely involved in the virulence process including immune escape, invasion, colonization, destruction of host tissues, and nutrient scavenging. Among the predicted virulence factors, type IX secretion-mediated and cell-surface...
Tipo: Text
Palavras-chave: Tenacibaculum maritimum; Fish pathogen; Virulence factors; Genome; Toxins.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00488/59939/63150.pdf

Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran
Provedor de dados: 80
Autores: Mousavi,Soolmaz; Dehkordi,Farhad Safarpoor; Rahimi,and Ebrahim.
Background Despite the high importance of Helicobacter pylori, the origin and transmission of this bacterium has not been clearly determined. According to controversial theories and results of previous studies, animal source foods – especially milk – play an important role in the transmission of H. pylori to humans. The aim of the present study was to determine the distribution of vacA, cagA,iceA and oipA virulence factors inH. pylori strains isolated from milk and dairy products and study their antimicrobial resistance properties.Methods A total of 520 raw milk and 400 traditional dairy product samples were cultured and tested. Those that were H. pylori-positive were analyzed for the presence of vacA,cagA, iceA and oipAvirulence factors. Antimicrobial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Virulence factors; Antibiotic resistance properties; Milk; Dairy products; Iran.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200341

Highly Pathogenic and Multidrug Resistant Avian Pathogenic Escherichia Coli in Free-Range Chickens from Brazil
Provedor de dados: 89
Autores: Oliveira,ES; Cardozo,MV; Borzi,MM; Borges,CA; Guastalli,EAL; Ávila,FA.
ABSTRACT Avian pathogenic Escherichiacoli (APEC) virulence mechanism has been continuously studied and it is believed to be multifactorial and because of this, this work aimed to characterize potentially APEC strains isolated from free-range hens. Isolates were submitted to PCR for the detection of virulence genes, which were of high prevalence. In vivo inoculation of day-old chicks revealed that 49 of these strains were of high and intermediate pathogenicity. In addition, isolates were submitted to antimicrobials susceptibility test with the majority of the strains presenting multiresistance. Phylogenetic analysis showed a greater presence of potentially APEC isolates in-group B2. In addition, high heterogeneity was detected among the isolates byXbaI...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: APEC; Backyard chickens; Colibacillosis; Pathogenicity; Virulence factors.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100326

Aeromonas species isolated from PINTADO fish (Pseudoplatystoma sp): virulence factors and drug susceptibility
Provedor de dados: 59
Autores: Rall,Vera Lúcia Mores; Iaria,Sebastião Timo; Heidtmann,Sandra; Pimenta,Fabiana Cristina; Gamba,Rosa Carvalho; Pedroso,Débora Midori Myaki.
Aeromonas has been described as an emergent foodborne pathogen of increasing importance. In this study, we report that 48% of 50 Pintado fish samples collected at the retail market of São Paulo city were positive for Aeromonas sp, as detected by the direct plating method. When the presence/absence method was used, the positivity was 42%. A. caviae was the most frequent species, followed by A. hydrophila and A. sobria. Production of cytotoxic enterotoxin, observed in suckling mouse assay, was detected in 67% of A. sobria strains, in 60% of A. hydrophila strains and in 40% of A. caviae strains. In vitro tests, performed with HEp-2 cells, showed that 88% of A. hydrophila, 27% of A. sobria and 13% of A. caviae strains were positive for this toxin. The in vivo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas sp; Virulence factors; Drug susceptibility; Fish.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300015

Chymotrypsin- and trypsin-like activities secreted by the multidrug-resistant yeasts forming the Candida haemulonii complex
Provedor de dados: 42
Autores: SOUTO,XÊNIA M.; BRANQUINHA,MARTA H.; SANTOS,ANDRÉ L.S.
Abstract: Candida haemulonii complex (C. haemulonii, C. haemulonii var. vulnera and C. duobushaemulonii) consists of emergent multidrug-resistant pathogens that cause bloodstream and deep-seated infections. However, little is known about their virulence factors. Herein, we evaluated the presence of extracellular serine peptidases in this fungal complex. Serine peptidase activity was measured by spectrophotometry using chromogenic peptide substrates to the S1 family. Chymotrypsin-, trypsin- and elastase-like activities were detected in all fungal isolates. Since higher chymotrypsin- and trypsin-like activities were observed from the cleavage of N-succinyl-Ala-Ala-Pro-Phe-pNa and N-benzoyl-Phe-Val-Arg-pNa, respectively, these substrates were selected for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian clinical isolates; Candida haemulonii complex; Chymotrypsin; Serine peptidases; Trypsin; Virulence factors.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500803

Prevalence of virulence factors in Escherichia coli strains isolated from the genital tract of healthy cows
Provedor de dados: 45
Autores: Resende,D.; Santo,E.; Macedo,C.; Marin,J.M.
The prevalence of virulence genes expressing fimbriae, production of hemolysin, colicin and aerobactin, was determined in Escherichia coli isolates from healthy cow’s genital tract not showing clinical signs of infection. The presence of fimbriae expression genes (pap, sfa, afa) was assayed using specific primers in a polymerase chain reaction; none were detected in any of the isolates. Yet, a prevalence of 90.4%, 69.8%, and 28.5% of virulence factors for colicin, hemolysin and aerobactin respectively, was detected in the isolates. Analysis of the bacterial pathogenicity of isolates from the bovine genital tract may contribute towards the understanding of E. coli behavior.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cow; Escherichia coli; Genital tract; Virulence factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300003

Pseudomonas Aeruginosa: Virulence Factors and Antibiotic Resistance Genes
Provedor de dados: 52
Autores: Rocha,Antônio José; Barsottini,Mario Ramos de Oliveira; Rocha,Renan Rhonalty; Laurindo,Maria Vitória; Moraes,Francisco Leandro Laurindo de; Rocha,Soraya Lília da.
Abstract In this review, we explore some aspects of Pseudomonas aeruginosa virulence factors that are related to disease development in healthy organisms and resistance to antibiotics. This pathogen is one of the most clinically and epidemiologically important bacteria in Brazil, being the major cause of opportunistic infections. Among the virulence factors, biofilm formation acting of manner different in the organism. Furthermore, we review several P. aeruginosa genes that act in antimicrobial resistance, such as β-lactamases against β-lactamers. The resistance to pied-lactamases in P. aeruginosa is associated to resistance to the broad-spectrum cephalosporin. On the other hand, there is a group of synthetic broad-spectrum antibiotics acting on DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Virulence factors; Antimicrobial activities; Biofilms; Β-lactamase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100902

Targeting of Virulence Factors and Plasmid Profiling of Klebsiella pneumoniae Causing Urinary Tract Infection in Sylhet City of Bangladesh
Provedor de dados: 52
Autores: Foysal,Md Javed; Majlish,Al-Nahian Khan; Islam,Kamrul; Alam,Md Jahangir; Ali,Md Hazrat; Momtaz,Farhana.
ABSTRACT Studies were conducted to characterize Klebsiella pneumoniae isolates from urinary tract infection (UTI) patients in Sylhet city of Bangladesh. At the same time, all isolates were screened for some common virulence genes and four significant isolates were searched for plasmid number and sizes by mini alkaline-lysis method. Among five tested isolates from female UTI patients, gyrase subunit B2 (gyrb2) amplified in all isolates, lipase and nuclease detected in three isolates and serine protease amplifies in two isolates and gave the expected band of 1130 bp, 517 bp, 1055 bp and 211 bp respectively. Two of four isolates showed 9.82 kb plasmid band on agarose gel. Isolates bearing 9.82 kb plasmid were found to be resistant to multiple commercial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: K. pneumoniae; Urinary tract infection; Virulence factors; Plasmid profiling; Correlation study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100423

Plesiomonas shigelloides exports a lethal cytotoxic-enterotoxin (LCE) by membrane vesicles
Provedor de dados: 55
Autores: Ludovico,Marilucia Santos; Martins,Luciano Moura; Bianco,Juares Ednaldo Romero; Andrade,Célia Guadalupe Tardelli de Jesus; Falcon,Rosabel; Joazeiro,Paulo Pinto; Gatti,Maria Silvia Viccari; Yano,Tomomasa.
ABSTRACT Plesiomonas shigelloides isolated from water in Brazil was previously described as a hemorrhagic heat-labile cytotoxic-enterotoxin producer. We purified this toxin from culture supernatants using ion metallic affinity chromatography (IMAC) followed by molecular exclusion chromatography. The pure toxin presented molecular mass of 50 kDa and isoelectric point (pI) around 6.9 by 2D electrophoresis. When injected intravenously, the purified cytotoxic-enterotoxin induced also severe spasms followed by sudden death of mice. Hence, we entitled it as lethal cytotoxic-enterotoxin (LCE). The presence of membrane vesicles (MVs) on cell surfaces of P. shigelloides was observed by scan electron microscopy (SEM). From these MVs the LCE toxin was extracted and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lethal cytotoxic enterotoxin (LCE); Membrane vesicles (MVs); Plesiomonas shigelloides; Toxin transportation; Virulence factors.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600546

Isolation, pathogenicity and disinfection of Staphylococcus aureus carried by insects in two public hospitals of Vitória da Conquista, Bahia, Brazil
Provedor de dados: 55
Autores: Oliveira,Pollianna S.; Souza,Simone G.; Campos,Guilherme B.; Silva,Danilo C.C. da; Sousa,Daniel S.; Araújo,Suerda P.F.; Ferreira,Laiziane P.; Santos,Verena M.; Amorim,Aline T.; Santos,Angelita M.O.G.; Timenetsky,Jorge; Cruz,Mariluze P.; Yatsuda,Regiane; Marques,Lucas M.
Currently, hospital infection is a serious public health problem, and several factors may influence the occurrence of these infections, including the presence of insects, which are carriers of multidrug-resistant bacterial species. The aim of this study was to isolate staphylococci carried by insects in two public hospitals of Vitoria da Conquista, Bahia and to identify the resistance profile, pathogenicity and efficacy of disinfection of the premises. A total of 91 insects were collected in 21 strategic points of these hospitals, and 32 isolated strains ofStaphylococcus aureus were isolated. Based on antibiogram and Minimum Inhibitory Concentration results, 95% of these strains were susceptible to oxacillin. These strains were also evaluated for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insects; Staphylococcus aureus; Resistance; Virulence factors; Disinfection.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200129

Biological activity of Serratia marcescens cytotoxin
Provedor de dados: 56
Autores: Carbonell,G.V.; Amorim,C.R.N.; Furumura,M.T.; Darini,A.L.C.; Fonseca,B.A.L.; Yano,T.
Serratia marcescens cytotoxin was purified to homogeneity by ion-exchange chromatography on a DEAE Sepharose Fast Flow column, followed by gel filtration chromatography on a Sephadex G100 column. The molecular mass of the cytotoxin was estimated to be about 50 kDa. Some biological properties of the cytotoxin were analyzed and compared with well-characterized toxins, such as VT1, VT2 and CNF from Escherichia coli and hemolysin produced by S. marcescens. The sensitivity of the cell lines CHO, HeLa, HEp-2, Vero, BHK-21, MA 104 and J774 to the cytotoxin was determined by the cell viability assay using neutral red. CHO and HEp-2 were highly sensitive, with massive cellular death after 1 h of treatment, followed by BHK-21, HeLa, Vero and J774 cells, while MA 104...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell culture; Biological activity; Serratia marcescens; Cytotoxin; Virulence factors.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300010

Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil
Provedor de dados: 56
Autores: Kerbauy,G.; Perugini,M.R.E.; Yamauchi,L.M.; Yamada-Ogatta,S.F.
In this study, we report the characterization of a strain of Enterococcus faecium vanA, which grows only in the presence of vancomycin (VDEfm-UEL). The bacterium was isolated from the feces of a female patient who had undergone surgical treatment of Reinke’s edema and was receiving intravenous vancomycin therapy for infection with methicillin/oxacillin-resistant Staphylococcus aureus, a postoperative complication. Antimicrobial dependence was further confirmed by the vancomycin E-test. VDEfm-UEL was also shown to be resistant to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, levofloxacin, penicillin, rifampicin, and teicoplanin. The putative virulence genes efaA, gelE and esp were detected by PCR. The ddl gene from VDEfm-UEL was cloned and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Enterococcus faecium; Vancomycin; Dependence; VanA genotype; Virulence factors.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300013

Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil
Provedor de dados: 56
Autores: Santos,A.C.M.; Zidko,A.C.M.; Pignatari,A.C.; Silva,R.M.
Most of the knowledge of the virulence determinants of extraintestinal pathogenicEscherichia coli (ExPEC) comes from studies with human strains causing urinary tract infections and neonatal meningitis and animal strains causing avian colibacillosis. In this research, we analyzed the phylogenetic background, the presence of 20 ExPEC virulence factors, and the intrinsic virulence potential of 74 E. coli strains isolated in São Paulo, Brazil, from 74 hospitalized patients (43 males and 31 females) with unknown-source bacteremia. Unlike other places in the world, the bacteremic strains originated equally from phylogroups B2 (35%) and D (30%). A great variability in the profiles of virulence factors was noted in this survey. Nevertheless, 61% of the strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteremia; Extraintestinal pathogenic Escherichia coli; Virulence factors; Intrinsic virulence potential; Phylogenetic background.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100968

Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens
Provedor de dados: 56
Autores: Carbonell,G.V.; Alfieri,A.F.; Alfieri,A.A.; Vidotto,M.C.; Levy,C.E.; Darini,A.L.C.; Yanaguita,R.M.
Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells. These strains were isolated from distinct clinical sources and classified into seven different serotypes (O1:H7; O4:NM; O10:NT; O19:NM; O6,14:H4; O6,14:NM and O6,14:H1). No relationship was observed between cytotoxic activity and clinical source or serotypes of the strains. Plasmids from five cytotoxin-producing S. marcescens strains were transferred to E. coli K12/711. The transconjugants did not exhibit cytotoxicity, indicating that the cytotoxic effect is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxin production; Nosocomial infections; Plasmids; Serratia marcescens; Virulence factors.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100005

Safety, beneficial and technological properties of Enterococcus faecium isolated from Brazilian cheeses
Provedor de dados: 58
Autores: Santos,Karina Maria Olbrich dos; Vieira,Antônio Diogo Silva; Salles,Hévila Oliveira; Oliveira,Jacqueline da Silva; Rocha,Cíntia Renata Costa; Borges,Maria de Fátima; Bruno,Laura Maria; Franco,Bernadette Dora Gombossy de Melo; Todorov,Svetoslav Dimitrov.
This study aimed to characterize the safety and technological properties of Enterococcus faecium strains isolated from Brazilian Coalho cheeses. High levels of co-aggregation were observed between Enterococcus faecium strains EM485 and EM925 and both Escherichia coli and Clostridium perfringens. Both strains presented low levels of hydrophobicity. E. faecium EM485 and EM925 were both able to grow in the presence of 0.5% of the sodium salts of taurocholic acid (TC), taurodeoxycholic acid (TDC), glycocholic acid (GC), and glycodeoxycholic acid (GDC), although they showed the ability to deconjugate only GDC and TDC. Both strains showed good survival when exposed to conditions simulating the gastro intestinal tract (GIT). When tested for the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterococcus faecium; Probiotic properties; Technological properties; Virulence factors; Antibiotic resistance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100237

Technology and safety assessment for lactic acid bacteria isolated from traditional Bulgarian fermented meat product "lukanka"
Provedor de dados: 58
Autores: Todorov,Svetoslav Dimitrov; Stojanovski,Saso; Iliev,Ilia; Moncheva,Penka; Nero,Luis Augusto; Ivanova,Iskra Vitanova.
Abstract The present work discusses the technological and new selection criteria that should be included for selecting lactic acid bacteria for production of fermented meat. Lactic acid bacteria isolated from Bulgarian traditional fermented "lulanka" salami was studied regarding some positive technological parameters (growth at different temperature, pH, and proteolytic activity). The presence of genes related to the virulence factors, production of biogenic amines, and vancomycin resistance were presented in low frequency in the studied lactic acid bacteria. On the other hand, production of antimicrobial peptides and high spread of bacteriocin genes were broadly presented. Very strong activity against L. monocytogenes was detected in some of the studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lukanka; Antibiotic resistance; Virulence factors; Lactic acid bacteria; Bacteriocins.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300576

Characterization of avian pathogenic Escherichia coli isolated from free-range helmeted guineafowl
Provedor de dados: 58
Autores: Borzi,Mariana Monezi; Cardozo,Marita Vedovelli; Oliveira,Elisabete Schirato de; Pollo,Andressa de Souza; Guastalli,Elisabete Aparecida Lopes; Santos,Luis Fernando dos; Ávila,Fernando Antonio de.
Abstract Avian pathogenic Escherichia coli (APEC) isolates from apparently healthy free range helmeted guineafowl were characterized. Most of them had a high frequency of virulence associated genes, multi drug resistance and high pathogenicity. We demonstrated that helmeted guineafowl have potential to transmit antibiotic resistant APEC to other species including humans.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: APEC; Virulence factors; Colibacillosis; Zoonotic potential; Numida meleagris.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500107

Virulence-associated characteristics of Enterococcus faecalis strains isolated from clinical sources
Provedor de dados: 58
Autores: Furumura,Márcia T.; Figueiredo,Patricia M.S.; Carbonell,Gleize V.; Darini,Ana Lucia da Costa; Yano,Tomomasa.
Thirty-two clinical isolates of Enterococcus faecalis were screened for virulence factors. Twenty-four (75%) isolates produced hemolysin on Mueller-Hinton blood agar plates with sheep erythrocytes. However, the cell free heat-stable hemolysin was detected in all isolates (100%) of E. faecalis when grown in BHI-GA (BHI medium supplemented with 1% glucose and 0.03% L-arginine), but not in BHI broth alone. Twenty-four isolates (75%) produced caseinase and 23 (71.9%) lipase, but none of the isolates produced gelatinase. Fifteen (46.9%) culture filtrates caused rounding and membrane alterations with blebbing formation followed by death in HeLa and HEp-2 cells, but not in Vero cells. Thirteen isolates (40.6%) agglutinated rabbit erythrocytes, but did not produce...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterococcus faecalis; Virulence factors; Hemolysin; Proteases; Lipase; Cytotoxin; Biofilm.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300007

Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Provedor de dados: 58
Autores: Martins,Katheryne Benini; Faccioli-Martins,Patricia Yoshida; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Fernandes,Simone; Oliveira,Aline A.; Dantas,Ariane; Zafalon,Luiz Francisco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535

Aeromonas associated diarrhoeal disease in south Brazil: prevalence, virulence factors and antimicrobial resistance
Provedor de dados: 58
Autores: Guerra,Ivani M.F.; Fadanelli,Raquel; Figueiró,Manuela; Schreiner,Fernando; Delamare,Ana Paula L.; Wollheim,Claudia; Costa,Sérgio Olavo P.; Echeverrigaray,Sergio.
Aeromonas were isolated from 27 (6.6%) of 408 patients admitted with acute gastroenteritis in two hospitals at Rio Grande do Sul, Brazil. Isolates were classified as A. hydrophila (51.8%), A. caviae (40.8%), and A. veronii biotype sobria (7.4%). The highest prevalence of Aeromonas associated infections occurred in lactants and children. Virulence genes (aerA -aerolysin/hemolysin, ahpA -serine-protease, satA - glycerophospholipid-cholesterol acyltransferase, lipA -lipase, and ahyB -elastase) and virulence factors (hemolytic, proteolitic, lipolitic activities, and biofilm formation) were identified in most A. hydrophila and A. veronii biotype sobria isolates, with lower frequencies on A. caviae. All Aeromonas isolates were resistant to ampicillin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas; Diarrhea disease; Virulence factors; Antimicrobial resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400011

Virulence factors and biofilm production by isolates of Bacteroides fragilis recovered from dog intestinal tracts
Provedor de dados: 58
Autores: Reis,Ana Catarina M.; Silva,Janice O.; Laranjeira,Bruno J.; Pinheiro,Adriana Q.; Carvalho,Cibele B.M.
Bacteroides fragilis colonizes dog guts both as a commensal and as an opportunistic pathogen. This study aims to evaluate virulence factors of 13 B. fragilis strains isolated from dog intestinal tracts and their ability for biofilm formation. Capsules were detected in all the evaluated strains. A total of 61.5% of all strains were biofilm producers. These attributes most likely play an important role in B. fragilis persistent colonization in the gut.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B. fragilis; Biofilm; Virulence factors; Dogs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200037

Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil
Provedor de dados: 58
Autores: Salvadori,Márcia Regina; Valadares,Geórgio Freesz; Leite,Domingos da Silva; Blanco,Jesús; Yano,Tomomasa.
Two hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type 1 (0.5%), and type 2 (4.4%), enterotoxins LT-II (8.3%) and STa (3.9%). No strain produced enterotoxin LT-I. Fimbrial adhesins F5 and F17 were produced by 7.3% and 4.8% of the strains, respectivly, and none expressed F41. Seven strains (3.4%) possessed the gene eae and belonged to serotypes O26:H-; O111:H- and O118:H16. These results suggest that calves in Brazil may be an important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calves; Diarrhea; E. coli; Virulence factors.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009

Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine
Provedor de dados: 58
Autores: Furian,Thales Quedi; Borges,Karen Apellanis; Laviniki,Vanessa; Rocha,Silvio Luis da Silveira; Almeida,Camila Neves de; Nascimento,Vladimir Pinheiro do; Salle,Carlos Tadeu Pippi; Moraes,Hamilton Luiz de Souza.
Abstract Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pasteurellosis; Virulence factors; Multiplex-PCR; Antimicrobial susceptibility.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100210

Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken
Provedor de dados: 58
Autores: Barbosa,Fernanda de Oliveira; Freitas Neto,Oliveiro Caetano de; Batista,Diego Felipe Alves; Almeida,Adriana Maria de; Rubio,Marcela da Silva; Alves,Lucas Bocchini Rodrigues; Vasconcelos,Rosemeire de Oliveira; Barrow,Paul Andrew; Berchieri Junior,Angelo.
ABSTRACT Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE ΔmotB and SE ΔfliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Flagellum; Virulence factors; Poultry; Gut colonisation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400754

Lack of association between genotypes and virulence factors in C. albicans strains isolated from vaginal secretion
Provedor de dados: 58
Autores: Shinobu,Cristiane Suemi; Ogatta,Sueli Fumie Yamada; Bizerra,Fenando; Furlaneto,Luciana; Peralta,Rosane Marina; Svidzinski,Terezinha Inez Estivalet; Consolaro,Marcia Edilaine Lopes.
The physiopathogenesis of vulvovaginal candidiasis (VVC) is still not completely elucidated. The objective of this study was to evaluate if there is a relationship between the different genotypes of Candida albicans, their main agent and the virulence of this yeast in vaginal isolates, and to check if there are laboratorial markers that can predict the ability of each isolate to develop VVC independently of symptoms. The production of exoenzymes protease, phospholipase and haemolysin, resistance to hydrogen peroxide, and the genotype were determined. Genotype A was predominant (75%), protease, phospholipase and haemolytic activity were highly expressed, and the majority of the yeasts were sensitive to H2O2 in 1 and 2 hours of exposure, suggesting that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. albicans; Vulvovaginal candidiasis; Genotype; Virulence factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300015

Molecular identification of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea in Londrina, Parana, Brazil
Provedor de dados: 58
Autores: Kobayashi,Renata K. T.; Saridakis,Halha Ostrensky; Dias,Angela M.G.; Vidotto,Marilda C.
In this work, the prevalence of enteropathogenic Escherichia coli (EPEC) in children in Londrina-PR, Brazil, was evaluated by means of digoxigenin-labelled DNA probes which identify the plasmid responsible for EPEC adherence factor (EAF), and virulence genes for EPEC as bundle-forming pilus (bfp) and E. coli attaching-effacing factor (eae). In addition, the isolated strains were serotyped and tested for adherence to HEp-2 cells. From 102 children with diarrhoea, 19 strains hybridized with at least one probe, and eleven of them were identified as typical EPEC because they hybridized with the three probes used, showed a localized adherence (LA) pattern, and presented no genes for enterotoxins (ST and LT) or invasion as detected by PCR. Six of the typical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteropathogenic Escherichia coli; EPEC; Digoxigenin-labelled DNA probes; Virulence factors; Serotypes; Diarrhoea.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400007

Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil
Provedor de dados: 58
Autores: Costa,Mateus Matiuzzi da; Drescher,Guilherme; Maboni,Franciele; Weber,Shana; Botton,Sônia de Avila; Vainstein,Marilene Henning; Schrank,Irene Silveira; Vargas,Agueda Castagna de.
The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasmids; Virulence factors; Antimicrobial resistance; Swine.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400027

Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer
Provedor de dados: 65
Autores: Agostinho,Juliana Maria Avanci; Cardozo,Marita Vedovelli; Borzi,Mariana Monezi; Marin,José Moacir.
ABSTRACT: Brazilian poultry industry generates large amounts of organic waste, such as chicken litter, which is often used in agriculture. Among the bacteria present in organic fertilizer are members of the Enterobacteriaceae family, such as Escherichia coli. The aim of this study was to analyze the profile of virulence factors and antimicrobial resistance of E. coli isolates from avian organic fertilizer. A total of 47 E. coli strains were tested through Polymerase chain reaction to detect virulence genes (hlyF, iss, ompT, iutA and iroN). Fourteen antimicrobials were used to test antimicrobial susceptibility in the strains. Genes characteristic of Avian Pathogenic E. coli (APEC) were reported among the strains, with the hlyF, iss and ompT genes being the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poultry production; Enterobacteriacea; Zoonotic risk; Virulence factors.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200451