Consulta: subjectFacets:"Virus"
Registros recuperados: 115
Data/hora: 03/06/2024 06:44:17

Etiología de un síndrome fungoso y viral en cladodios de nopal verdura (Opuntia ficus-indica).
Provedor de dados: 32
Autores: Alonso Barrera, Berenice.
El cultivo de nopal (Opuntia ficus-indica) presenta diversas enfermedades de importancia económica asociadas a hongos y virus. Los objetivos de esta investigación fueron: a) detectar la ocurrencia de infecciones latentes del agente causal de la mancha negra, Pseudocercospora opuntiae (Po), mediante estudios histológicos y evaluar la implicación sinergética de Colletotrichum sp. (C) en la infección de P.opuntiae, y b) demostrar la naturaleza etiológica viral asociada a manchas cloróticas anulares en nopal. Con respecto al primer objetivo se evaluaron 31200 cortes histológicos provenientes de 78 cladodios sintomáticos colectados en siete fechas de muestreo comprendido en el periodo, junio-agosto, caracterizado por alta incidencia de mancha negra....
Palavras-chave: Pseudocercospora; Colletotrichum; Virus; Infección latente; Sinergismo; Maestría; Fitopatología.
Ano: 2011
URL: http://hdl.handle.net/10521/525

Virus asociados al cultivo de jitomate en el estado de Morelos.
Provedor de dados: 32
Autores: Pérez Rojas, Moisés.
En un invernadero comercial de jitomate ubicado en Zacatepec, Morelos, se realizaron aplicaciones independientes de miel de abeja, Saccharomyces cerevisiae, ácido acetil salicílico, cobre-manganeso-zinc y acibenzolar S-metil para determinar su efecto sobre el rendimiento, incidencia y severidad de virosis en este cultivo. Adicionalmente, se evaluó el extracto de clavo como repelente de mosca blanca. Las plantas tratadas con levadura tuvieron la menor incidencia en la primera fecha de evaluación. Plantas asperjadas con acibenzolar S-metil mostraron la menor severidad en las tres primeras fechas de evaluación, mientras que las tratadas con miel presentaron los valores más altos de incidencia en todas las fechas de evaluación. El extracto de clavo no funcionó...
Palavras-chave: Solanum lycopersicum; Virus; Incidencia; Severidad; Rendimiento; Control; Incidence; Severity; Yield; Fitopatología; Maestría.
Ano: 2013
URL: http://hdl.handle.net/10521/2000

Etiología de un síndrome fungoso y viral en cladodios de nopal verdura (Opuntia ficus-indica).
Provedor de dados: 32
Autores: Alonso Barrera, Berenice.
El cultivo de nopal (Opuntia ficus-indica) presenta diversas enfermedades de importancia económica asociadas a hongos y virus. Los objetivos de esta investigación fueron: a) detectar la ocurrencia de infecciones latentes del agente causal de la mancha negra, Pseudocercospora opuntiae (Po), mediante estudios histológicos y evaluar la implicación sinergética de Colletotrichum sp. (C) en la infección de P.opuntiae, y b) demostrar la naturaleza etiológica viral asociada a manchas cloróticas anulares en nopal. Con respecto al primer objetivo se evaluaron 31200 cortes histológicos provenientes de 78 cladodios sintomáticos colectados en siete fechas de muestreo comprendido en el periodo, junio-agosto, caracterizado por alta incidencia de mancha negra....
Palavras-chave: Pseudocercospora; Colletotrichum; Virus; Infección latente; Sinergismo; Maestría; Fitopatología.
Ano: 2011
URL: http://hdl.handle.net/10521/525

Wild Bird Movements and Avian Influenza Risk Mapping in Southern Africa
Provedor de dados: 7
Autores: Cumming, Graeme S.; Percy FitzPatrick Institute, DST/NRF Center of Excellence, University of Cape Town, Rondebosch, 7701, South Africa; graeme.cumming@uct.ac.za; Hockey, Philip A. R.; Percy FitzPatrick Institute, DST/NRF Center of Excellence, University of Cape Town, Rondebosch, 7701, South Africa; phil.hockey@uct.ac.za; Bruinzeel, Leo W.; Percy FitzPatrick Institute, DST/NRF Center of Excellence, University of Cape Town, Rondebosch, 7701, South Africa; lbruinze@adu.uct.ac.za; Du Plessis, Morne A.; Percy FitzPatrick Institute, DST/NRF Center of Excellence, University of Cape Town, Rondebosch, 7701, South Africa; mornedup@vodamail.co.za.
Global analyses of the potential for avian influenza transmission by wild birds have ignored key characteristics of the southern African avifauna. Although southern Africa hosts a variety of migratory, Holarctic-breeding wading birds and shorebirds, the documented prevalence of avian influenza in these species is low. The primary natural carriers of influenza viruses in the northern hemisphere are the anatids, i.e., ducks. In contrast to Palearctic-breeding species, most southern African anatids do not undertake predictable annual migrations and do not follow migratory flyways. Here we present a simple, spatially explicit risk analysis for avian influenza transmission by wild ducks in southern Africa. We developed a risk value for each of 16 southern...
Tipo: Peer-Reviewed Reports
Palavras-chave: Anatidae; Avian influenza; Botswana; Ducks; Influenza; Landscape ecology; Namibia; Pathogen; South Africa; Virus; Waterfowl; Zimbabwe.
Ano: 2008

Medicina de la conservación, enfermedades y aves rapaces
Provedor de dados: 68
Autores: Saggese,Miguel D.
Como resultado de los cambios de origen humano en los ambientes naturales, del sostenido aumento de la población humana mundial y de la creciente tasa de contacto entre seres humanos, animales silvestres y domésticos, la exposición de todos ellos a macroparásitos (helmintos y artrópodos) y microparásitos (bacterias, virus, protozoos) está en constante aumento. Esto conduce a la aparición de numerosas enfermedades en animales y seres humanos. Sumados a los factores clásicamente considerados (pérdida de hábitat, introducción de especies, efecto en cadena de las extinciones y persecución directa), los parásitos pueden ocasionar también severos cambios demográficos en poblaciones de aves rapaces y otros animales silvestres, y contribuir a su declinación. Como...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artrópodos; Bacterias; Conservación; Enfermedades; Falconiformes; Helmintos; Hongos; Medicina; Protozoos; Strigiformes; Virus.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0073-34072007000200004

Novel antiviral activity of dialdehyde starch
Provedor de dados: 69
Autores: Song,Le; Cruz,Caroline; Farrah,Samuel R; Baney,Ronald H.
A significant effort worldwide is being directed toward development of novel biocides against drug-resistant bacterial and viruses because of the significant potential human infection risks in the general population. We report here the discovery of a strong antiviral biocide, dialdheyde starch (DAS). Antiviral tests were carried out against three non-envelop viruses, including two bacterial viruses MS2 and PRD1, and one human virus Poliovirus. Dialdehyde starch aqueous suspensions were effective biocides against these three test viruses in a 1 hr exposure test. The antiviral activity was significantly enhanced in a four-hour exposure test, with maximum seven orders of magnitude reductions against MS2 and PRD1, and four-order reduction against Poliovirus....
Tipo: Journal article
Palavras-chave: Antiviral; Biocide; Dialdehyde starch; Virus.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200013

Detecção de vírus de macieiras e pereiras por IC-RT-PCR usando anticorpos policlonais contra proteínas virais expressadas em Escherichia coli.
Provedor de dados: 14
Autores: NICKEL, O.; FAJARDO, T. V. M.
bitstream/item/170378/1/Comunicado-Tecnico-182.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Anticorpos policlonais; Proteínas virais; Macieira.; Maca; Virus; Pera; Escherichia Coli.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1043254

Vírus latentes aumentam a suscetibilidade de macieira cv. Maxi Gala à mancha foliar de Glomerella.
Provedor de dados: 14
Autores: ALVES, S. A. M.; NICKEL, O.; FAJARDO, T. V. M.
bitstream/item/150628/1/Comunicado-Tecnico-188.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Apple stem pitting virus (ASGV); Apple chlorotic leaf spot virus (ACLSV); Doenças fúngicas; Macieira.; Maca; Virus; Doenca de planta; Fungo.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1057185

Viroses desafiadoras.
Provedor de dados: 14
Autores: NOGUEIRA, I.; CARVALHO, R. de C. P.; BOITEUX, L. S.; FONSECA-BOITEUX, M. E. de N.
Crinivírus, transmitidos por mosca-branca, engrossam a lista de viroses enfrentadas pelos produtores de tomate no Brasil e se somam a desafios como a incidência de Begomovírus e Tospovírus. A busca por cultivares resistentes tem se mostrado indispensável diante da ampla gama de hospedeiros e das limitações no controle do inseto-vetor.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Tomate; Virus; Virose vegetal; Inseto; Praga; Mosca branca; Variedade resistente; Begomovirus; Crinivirus; Tospovirus.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1029787

Manejo regional.
Provedor de dados: 14
Autores: INOUE-NAGATA, A. K.; MACEDO, M. A. de; SOUZA, J. O. de; MICHEREFF FILHO, M.
A begomovirose ou geminivirose, transmitida por Bemisia tabaci, é um dos piores pesadelos enfrentados pelos produtores de tomate. Altamente dependente da população de moscas brancas preentes na área, a doença exige medidas integradas contra o inseto-vetor, de forma consciente, planejada e organizada entre os produtores, considerando todo o sistema agrícola da região.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Bergomovirose; Planta tiguera; Doença de planta; Mosca branca; Virus; Transplante de planta; Tomate; Erva daninha.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/992483

Vírus.
Provedor de dados: 14
Autores: NICKEL, O.; FAJARDO, T. V. M.
Tipo: Parte de livro
Palavras-chave: Vírus da pereira; Enxertia; Doença de planta; Virus; Pera.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1040125

Controle de doenças causador por vírus.
Provedor de dados: 14
Autores: FAJARDO, T. V. M.
São conhecidas na videira (Vitis spp.) cerca de sessenta doenças consideradas de origem viral (Tabela 1). A videira, por ser propagada vegetativamente, facilita a disseminação desses patógenos e favorece o aparecimento de doenças complexas, pelo acúmulo de diferentes vírus numa mesma planta. Muitas dessas doenças estão bem identificadas e caracterizadas, enquanto outras dependem ainda de estudos complementares para definir sua natureza etiológica. Algumas ocorrem de forma ocasional na videira, aparentemente sem expressão econômica. Outras, embora causem prejuízos econômicos importantes, estão restritas a determinadas regiões ou países, possivelmente condicionadas por certas características regionais, como o plantio de cultivares sensíveis ou devido a...
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Doenças da videira; Doenças de oriegem viral; Uvas finas; Videira.; Doenca de planta; Virus; Praga.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1060283

CADEIA produtiva do alho ganha nova cultivar.
Provedor de dados: 14
bitstream/item/136814/1/digitalizar0031.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Cultivar Amarante; Alho; Variedade resistente; Virus.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1033300

Processo de formulação do baculovirus spodoptera em pó molhável.
Provedor de dados: 14
Autores: VALICENTE, F. H.; TUELHER, E. de S.; BARROS, E. C. de.
bitstream/item/31847/1/circ-156.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Virus; Controle biológico.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/884430

RNAi: uma estratégia a ser explorada para a indução de resistência a viroses em tomateiro.
Provedor de dados: 14
Autores: RÊGO, C. M.; NAGATA, A. K. I.; NAKASU, E. Y. T.
bitstream/item/152688/1/edicao20-inoueNagata.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: RNA interferente; Mosca-branca.; Virus; Doença de planta; Inseticida; Variedade resistente.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1059831

Embrapa 5.1: o feijoeiro geneticamente modificado resistente ao mosaico dourado.
Provedor de dados: 14
Autores: FARIA, J. C. de; ARAGÃO, F. J. L.
Introdução. Caracterização do agente causal. Transgênicos. O feijoeiro Embrapa 5.1. Construção gênica utilizada. Metodologia de transformação. Métodos utilizados para a modificação genética. Plantas transgênicas obtidas. Análise da resistência ao BGMV. Experimento de campo. Biossegurança. Principais considerações. Informações relativas ao OGM. Caracterização agronômica. Avaliação de risco à saúde humana e animal. Segurança alimentar. Alimentação animal. Segurança Ambiental. Perspectivas. Referências.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Feijão; Organismo transgênico; Planta transgênica; Mosaico dourado; Doença de planta; Virus; Biotecnologia; Engenharia genética; Biossegurança.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/974491

Cultura de tecidos no controle de viroses da bananeira no Brasil.
Provedor de dados: 14
Autores: SILVA NETO, S. P. da; SILVA, M. S.
bitstream/CPAC-2010/31580/1/doc-274.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Plant diseases; Tropical fruits.; Banana; Musa acuminata; Fruta tropical; Doença de planta; Virus; Bananas; Viruses.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/697556

Enrolamento da folha da videira (Grapevine leafroll-associated virus 3).
Provedor de dados: 14
Autores: EIRAS, M.; FAJARDO, T. V. M.
bitstream/item/152449/1/Cultura-videira-INSTITUTO-BIOLOGICO-Guia-de-Sanidade-Vegetal.pdf
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Folha da videira; Enrolamento da folha da videira; Videira.; Virus; Doenca de planta; Vitis Vinifera.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1059489

Limpeza clonal de batata-doce: produção de matrizes com elevada qualidade fitossanitária.
Provedor de dados: 14
Autores: FERNANDES, F. R.
bitstream/item/81876/1/ct-117.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Limpeza clonal; Batata-doce.; Qualidade; Virus; Semente; Limpeza de semente; Ipomoea batatas.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/956451

Produção de alho-semente livre de vírus em pequenas propriedades.
Provedor de dados: 14
Autores: RESENDE, F. V.; MELO, W. F. de; GUIDUCCI FILHO, E.; DUSI, A. N.
bitstream/item/57208/1/CT-99.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Alho; Produção; Semente; Virus; Agricultura familiar.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/916712

Levantamento de doenças e mosca-branca em tomateiro em regiões produtoras no Brasil.
Provedor de dados: 14
Autores: QUEZADO-DUVAL, A. M.; INOUE-NAGATA, A. K.; REIS, A.; PINHEIRO, J. B.; LOPES, C. A.; ARAÚJO, E. R.; FONTENELLE, M. R.; COSTA, J. R.; GUIMARÃES, C. M. N.; ROSSATO, M.; BECKER, W. F.; COSTA, H.; FERREIRA, M. A. S. V.; DESTÁFANO, S. A. L.
No intuito de estudar quais as principais doenças para os cultivos nacionais, realizou-se um levantamento no período de 2008 a 2011 em diferentes regiões produtoras no Brasil, que abrangeu 93 lavouvas de tomate para mesa em 12 estados e no Distrito Federal e 51 lavouras nos três estados produtores de tomate para processamento industrial e no Distrito Federal.
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Tomate; Doença de planta; Praga; Inseto; Mosca branca; Virus; Bacteria; Fungo; Nematoide.; Solanum lycopersicum.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/974139

Viroses em batata-doce: principais agentes.
Provedor de dados: 14
Autores: FERNANDES, F. R.
bitstream/item/91495/1/digitalizar0065.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Batata-doce.; Doença de planta; Virus; Produtividade; Ipomoea batatas.; Sweet potato chlorotic fleck virus; Sweet potato chlorotic stunt virus; Sweet potato feathery mottle virus; Sweet potato latent virus.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/969436

Crinivírus e geminivírus transmitidos por mosca branca: ameaça à produção nacional de batata.
Provedor de dados: 14
Autores: LIMA, M. F.
bitstream/item/143089/1/Milza-BShow-1642016.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Bemisia tabaci; Praga; Inseto; Solanum tuberosum; Virus.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1045175

Guia para o reconhecimento e manejo da mosca-branca, da geminivirose e da crinivirose na cultura do tomateiro.
Provedor de dados: 14
Autores: MICHEREFF FILHO, M.; INOUE-NAGATA, A. K.
Esta Circular Técnica apresenta informações sobre os vírus transmitidos pelas moscas-brancas ao tomateiro, a forma de transmissão e os sintomas das doenças, assim como as formas de monitoramento e medidas de controle das moscas-brancas.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Tomate; Praga; Inseto; Virus; Controle químico; Variedade resistente.; Solanum lycopersicum.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1021765

Embrapa vai produzir mudas de videiras livres de vírus
Provedor de dados: 14
Autores: MELO, N. F. de.
A infecção de plantas por vírus é um dos problemas mais sérios que afetam a produtividade dos parreirais nos quatro cantos do planeta. Responsável por perdas que podem chegar a 100%, preocupa agricultores e pesquisadores de especialidades e de lugares diversos. No Submédia do Vale do rio São Francisco, a Embrapa Semiárido e a Cooperativa Agrícola de Juazeiro (CAJ) firmaram convênio para contornar esse problema, produzindo mudas isentas desses microrganismos. Análises recentes feitas em variedades
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Uvas sem sementes; Thompson; Superior; Crimson Seedless; GLRaV-3; Enrolamento das folhas.; Uva; Doença; Virus; Biotecnologia; Muda.; Grapes.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1059076

Guia de identificacao das doencas do tomateiro.
Provedor de dados: 14
Autores: LOPES, C. A.
Requeima; Pinta-preta; Septoriose; Mancha-de-estenfilio; Oidio; Podridao-de-esclerotinia; Rizoctoniose; Vira-cabeca; Mosaico-dourado; Murcha-bacteriana; Cancro-bacteriano; Pinta-bacteriana; Mancha-bacteriana; Talo-oco ou podridao-mole; Galhas ou nematoide.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Oidiopsis sicula; Clavibacter michiganensis michiganensis; Pseudomonas syringae tomato; Xanthomonas campestris vesicatoria.; Tomate; Doença de planta; Fungo; Bacteria; Virus; Nematoide; Alternaria solani; Septoria lycopersici; Sclerotinia Sclerotiorum; Rhizoctonia Solani; Ralstonia Solanacearum; Meloidogyne spp; Phytophthora Infestans.; Tomatoes; Erysiphe; Nematoda; Solanum lycopersicum.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/768979

Viroses de cucurbitáceas.
Provedor de dados: 14
Autores: LIMA, M. F.
O objetivo desta Circular Técnica é descrever as principais viroses que afetam espécies de cucrbitáceas no Brasil, quanto aos sintomas, etiologia, epidemiologia e medidas de controle.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Melon yellowing associated virus; Cucurtita moschata.; Melão; Melancia; Abóbora; Pepino; Maxixe; Chuchu; Doença de planta; Virus; Controle cultural; Cucumis melo; Citrullus lanatus; Cucumis sativus; Cucumis anguria; Sechium edule.; Cucumber mosaic virus; Potyvirus; Squash mosaic virus; Watermelon mosaic virus; Zucchini lethal chlorosis virus; Zucchini yellow mosaic virus.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/916636

Doença vira-cabeça em alface: sintomatologia, transmissão, epidemiologia e medidas de controle.
Provedor de dados: 14
Autores: LIMA, M. F.; MICHEREFF FILHO, M.; BOITEUX, L. S.; SUINAGA, F. A.
bitstream/item/152753/1/CT-153-PARA-INTERNET.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Vira-cabeça; Alface; Lactuca sativa; Virus; Doença de planta.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1059924

Medidas gerais de controle das viroses em alho.
Provedor de dados: 14
Autores: FERNANDES, F. R.
bitstream/item/74810/1/Nosso-alho.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Diagnose; Alho-semente; Allim sativum.; Alho; Doença de planta; Virus; Praga de planta; Inseto; Pulverização.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/945806

Evidence of the presence of viral shellfish contamination in shellfish after short rainfall events
Provedor de dados: 5
Autores: Le Saux, Jean-claude; Serais, Ophelie; Krol, Joanna; Parnaudeau, Sylvain; Salvagnac, P.; Delmas, G.; Cicchelero, V.; Claudet, J.; Pothier, P.; Balay, K.; Fiandrino, Annie; Pommepuy, Monique; Le Guyader, Soizick.
Infectious diseases linked to the consumption of raw shellfish have long been identified. Over the past century, various strategies have been set up in shellfish growing areas throughout the world to guarantee the sanitary quality of shellfish and to protect consumers. However despite sanitary improvements, human enteric viruses - especially Hepatitis A virus and norovirus– have been found to be associated with shellfish outbreaks. A recent example demonstrated the impact of storm events. Following heavy rain and sewage overflow, shellfish beds were contaminated and the shellfish from them were marketed after depuration. However, since viruses persist longer than fecal contamination indicator bacteria, several clusters of gastroenteritis cases were...
Tipo: Text
Palavras-chave: Virus; Shellfish; Contamination events.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00066/17736/15257.pdf

Use of rotavirus virus-like particles as surrogates to evaluate virus persistence in shellfish
Provedor de dados: 5
Autores: Loisy, Fabienne; Atmar, R; Le Saux, Jean-claude; Cohen, J; Caprais, Marie-paule; Pommepuy, Monique; Le Guyader, Soizick.
Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log(10) of VLPs was depurated after 1 week in warm seawater (22 degrees C). Phage MS2 was depurated more rapidly (about 2 log(10) in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10(10) and 10(9) particles per g of pancreatic tissue)...
Tipo: Text
Palavras-chave: Seawater; Viral diseases; Bioaccumulation; Depuration; Contamination; Model; Pancreas; Regression analysis; Shellfish; Phages; Virus.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1233.pdf

Pathogens in aquaculture : specific pathogens or human contamination risks
Provedor de dados: 5
Autores: Renault, Tristan.
The vatness of aquaculture ventures worldwide has taken impressive leaps durint the past three decades, and a large portion of this pertains to mariculture. Significant strides have been made in the design and implementation of more efficient and cost effective growing areas, feed, and harvesting techniques. Moreover the economic impact of aquaculture will certainly increase in the years ahead.
Tipo: Text
Palavras-chave: Mycoplasmas; Chlamydiae; Rickettsia; Bacteria; Virus; Aquaculture; Pathogens.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2899.pdf

Shellfish viruses
Provedor de dados: 5
Autores: Renault, Tristan.
Mortalities in a number of commercially important mollusc species have been associated with the detection of viruses belonging to several families. The first description of a virus was in adult eastern oyters, Crassostrea virginica, with the detection of virus particles resembling members of the family Herpesviridae. Subsequently, mass mortalities in French stocks of adult Portuguese oysters, C. angulata, were associated with irido-like virus infection. Other viruses observed in molluscs are described as members of the families Iridoviridae, Papovaviridae and Reoviridae. Little information is available on viral infections that affect molluscs due to primarily to the inadequacy of diagnostic methods that are employed when mass mortality events occur. Most...
Tipo: Text
Palavras-chave: Virus; Scallops; Picornavirus; Oyter herpesvirus 1; Oysters; Molluscs; Irido Like virus; Diseases; Herpesvirus; Clams; Bivalves; Birnavirus; Abalones.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4932.pdf

Etude de l'herpès virus de l'huître - Fonds d'Aide à la Recherche et à l'Innovation - Décision d'Aide à la Recherche n° 95-07-01 - Rapport intermédiaire 1998
Provedor de dados: 5
Autores: Renault, Tristan.
Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les outils de diagnostic développés sont basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR). Ce projet, proposé sur une durée de trois années (1995-1998),...
Tipo: Text
Palavras-chave: Virus; Pathologie; Herpès virus; Crassostrea gigas; Pathogène; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15450/12825.pdf

Purification and characterization of infectious myonecrosis virus of penaeid shrimp
Provedor de dados: 5
Autores: Poulos, Bonnie; Tang, K; Pantoja, C; Bonami, Jean-robert; Lightner, D.
The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1(.)366 g ml(-1) in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5' ORF (ORF 1, nt 136-4953)...
Tipo: Text
Palavras-chave: Purification; Myonecrosis; Virus; Shrimp; Penaeid.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1504.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text
Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par des virus. Sous-région marine Manche - Mer du Nord. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Pommepuy, Monique.
Tipo: Text
Palavras-chave: Manche; Mer du Nord; Coquillages; Virus; Risques; Sources de contamination; Impacts; Suivi.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00328/43875/43449.pdf

Viruses of bivalve shellfish
Provedor de dados: 5
Autores: Renault, Tristan.
Bivalve molluscs are filter feeders and as a consequence they may bioaccumulate in their tissues viruses that infect humans and higher vertebrates. However, there have also been described mortalities of bivalve molluscs associated with viruses belonging to different families. Mass mortalities of adult Portuguese oysters, Crassostrea angulata, among French livestocks (between 1967 et 1973) were associated with irido-like virus infections. Herpesviruses were reported in the eastem oyster, Pacific oyster and European flat oyster and lately in scallops and clams. Disseminated neoplasia, a proliferative cell disorder of the circulatory system of bivalves, although of uncertain aetiology, has been suggested to be caused by retroviral infections. Other viruses...
Tipo: Text
Palavras-chave: Infection; Virus; Mollusc; Bivalve; Infection; Virus; Mollusque; Bivalve.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2925.pdf

Etude de l'herpès virus de l'huître. Fonds d'Aide à la Recherche et à l'Innovation. Décision d'Aide à la Recherche n° 95-07-01 (Rapport intermédiaire)
Provedor de dados: 5
Autores: Renault, Tristan.
L'Unité de Recherche en Pathologie et Immunologie Générales (Station IFREMER de La Tremblade) se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occassionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années...
Tipo: Text
Palavras-chave: Virus; Herpès; Pathogène; Purification; Herpesviridae; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00043/15447/12818.pdf

Transmission expérimentale du virus de type herpes chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Arzul, Isabelle.
Au cours de l'été 1991, d'importantes mortalités de larves d'huître creuse, Crassostrea gigas, ont été observées dans des écloseries, simultanément en France (Comps et Cochennec, 1993) et en Nouvelle-Zélande (Hine &amp; al., 1992).  Les examens histologiques ainsi que des examens réalisés en microscopie électronique à transmission d'échantillons de larves présentant des mortalités ont permis de détecter la présence de particules virales associées à des lésions cellulaires.  D'après la morphologie, la taille, les localisations intranucléaires et cytoplasmiques du virus, il est possible d'envisager son appartenance à la famille des Herpesviridae.  De nouvelles mortalités sporadiques ont été observées au cours des étés suivants sur des lots de larves élevées...
Tipo: Text
Palavras-chave: Virus; Herpesviridae; Bivalve; Crassostrea gigas; Etiologie; Epidémiologie; Diagnostic; Reproduction infection.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00106/21711/19289.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 96 RPC R70 - Programme Pathologie - Rapport Scientifique année 1996
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Lipart, Cecile; Thebault, Anne.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00043/15452/12827.pdf

Infection virale associée à des mortalités chez l'huître Crassostrea gigas Thunberg
Provedor de dados: 5
Autores: Comps, Michel; Bonami, Jean-robert.
Some viral particles close to the lridovirus are described in the cytoplasm of some connective cells in the Japanese oyster Crassostrea gigas. This virus looks similar to the one described in Crassostrea angulata and associated with the mortalities of 1970-1973 and to the one observed in the gill damages in the same species.
Tipo: Text
Palavras-chave: Virus; Mortalité; Pathologie; Crassostrea gigas; Huître.
Ano: 1977
URL: http://archimer.ifremer.fr/doc/1977/publication-6082.PDF

Influence of low temperatures on the survival of the Pacific oyster (Crassostrea gigas) infected with Ostreid herpesvirus type 1
Provedor de dados: 5
Autores: Pernet, Fabrice; Tamayo, David; Petton, Bruno.
Mortalities of oyster seed of Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have been observed in many oyster producing countries since 2008. The objective of this study was to further investigate whether low temperature treatments can offer a viable option to mitigate oyster mortalities. An experiment was set-up to further evaluate the effect of low temperature treatments (10 and 13°C vs. 21°C) and their duration (6 d to 83 d) on the survival of oysters previously infected with OsHV-1 μVar by means of exposure to field conditions in areas where mortalities were occurring. Average survival of oysters infected with OsHV-1μVar was 71% after 83 d at low temperatures compared to only 23% in controls maintained at 21°C. During...
Tipo: Text
Palavras-chave: Bivalve; Disease; OsHV-1; Oyster; Temperature; Virus.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00259/37047/35555.pdf

Recherches histologiques et cytologiques sur les infections intracellulaires des mollusques bivalves marins
Provedor de dados: 5
Autores: Comps, Michel.
Trois types d'infections intracellulaires des molluques bivalves marins sont étudiés dans leurs aspects histologiques et ultrastructuraux. La première partie traite de deux maladies causées par des virus ADN intracytoplasmiques chez les huîtres Crassostrea angulata et Crassostrea gigas : la maladie des branchies et les mortalités massives de 1970 à 1977. Les caractéristiques morphologiques et ultrastructurales de vrions, leur morphogénèse et les effets pathogènes sont décrits et développés dans une étude comparative des deux types de viroses. Dans la seconde partie sont abordées les infections rickettsiennes des Pélécypodes marins avec la description de procaryotes appartenant aux Rickettsiales et d'agents infectieux à cycle de développement...
Tipo: Text
Palavras-chave: Mollusques bivalves marins; Infections intra-cellulaires; Virus; Iridovirus; Rickettsies; Cycle chlamydien; Protiste; Histologie; Ultrastructure.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/00422/53365/54209.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf

The role of models in assessing the impact of sewage overflows on feacal water contamination
Provedor de dados: 5
Autores: Riou, Philippe; Le Saux, Jean-claude; Dumas, Franck; Le Guyader, Soizick; Le Goff, R.; Maheux, Frank; Lamort, Laure; Pommepuy, Monique.
Climate change contributes to local weather modifications such as storm events and heavy rainfall, and in particular, changes in the frequency and intensity of extreme storm and rainfall events. Observations have indicated that rainfall-runoff processes, like sewage overflow may induce fecal contamination in coastal water. Pathogens present in runoff water could therefore contaminate shellfish and be responsible for food-borne disease outbreaks. Mathematical models for this were used to predict the effect of climate modifications on shellfish quality. Using a hydrodynamic model, simulations of water quality conditions under a variety of storm event scenarios were run and are presented here for a harvesting area in France. Major storm events occurring in...
Tipo: Text
Palavras-chave: Modeling; Seawater fecal contamination; E. coli; Virus; Shellfish; Climate change.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00066/17739/15261.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 95 RPC R 62 - Programme Pathologie - Rapport Scientifique année 1995
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Thuillier, Benoit.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00043/15453/12828.pdf

Performance of viruses and bacteriophages for fecal source determination in a multi-laboratory, comparative study
Provedor de dados: 5
Autores: Harwood, Valerie J.; Boehm, Alexandria B.; Sassoubre, Lauren M.; Vijayavel, Kannappan; Stewart, Jill R.; Fong, Theng-theng; Caprais, Marie-paule; Converse, Reagan R.; Diston, David; Ebdon, James; Fuhrman, Jed A.; Gourmelon, Michele; Gentry-shields, Jennifer; Griffith, John F.; Kashian, Donna R.; Noble, Rachel T.; Taylor, Huw; Wicki, Melanie.
An inter-laboratory study of the accuracy of microbial source tracking (MST) methods was conducted using challenge fecal and sewage samples that were spiked into artificial freshwater and provided as unknowns (blind test samples) to the laboratories. The results of the Source Identification Protocol Project (SIPP) are presented in a series of papers that cover 41 MST methods. This contribution details the results of the virus and bacteriophage methods targeting human fecal or sewage contamination. Human viruses used as source identifiers included adenoviruses (HAdV), enteroviruses (EV), norovirus Groups I and II (NoVI and NoVII), and polyomaviruses (HPyVs). Bacteriophages were also employed, including somatic coliphages and F-specific RNA bacteriophages...
Tipo: Text
Palavras-chave: Virus; Bacteriophage; Water quality; Fecal pollution; Validation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28197/27786.pdf

Les virus des crevettes pénéides tropicales. 1ère partie : Symptomatologie-Epidémiologie, Diagnostic sur le terrain, Diagnostic histologique
Provedor de dados: 5
Autores: Weppe, Maurice; Aquacop.
Ce rapport fait le point sur nos connaissances, et les résultats des expérimentations menées au COP sur les virus des pénéides. Sont également inclus des détails sur les méthodes de diagnostic sur le terrain ainsi que les conseils pour l'expédition de matériel vers les laboratoires de diagnostic.
Tipo: Text
Palavras-chave: Peneides; Virus; Epidemiologie; Histologie.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00180/29112/27517.pdf

Etude de l'herpès virus de l'huître - Fonds d'Aide à la Recherche et à l'Innovation - Décision d'Aide à la Recherche n° 95-07-01 - Rapport Final Août 1998
Provedor de dados: 5
Autores: Renault, Tristan.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en œuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En effet,...
Tipo: Text
Palavras-chave: Virus; Pathologie; Herpès virus; Crassostrea gigas; Pathogène; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15451/12826.pdf

Etude du virus de type herpes observé chez les huîtres
Provedor de dados: 5
Autores: Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Delsert, Claude; Cochennec, Nathalie; Renault, Tristan.
Since 1991, sporadic, but high mortality in larvae of the Pacific oyster (Crassostrea gigas) has been observed during the summer in various French hatcheries. This mortality was concomitant with the detection of a virus by transmission electron microscopy. This virus, by virtue of its morphological characteristics and developmental cycle, seems to belong to the Herpesviridae family. The pathogenicity of this viral agent has been demonstrated in the laboratory by experimentally induced mortality on healthy larvae. Moreover, concomitant mortality has been reported in larvae of Crassostrea gigas and Ostrea edulis reared in the same tanks, with detection of a herpes-type virus.
Tipo: Text
Palavras-chave: Virus; Herpeviridae; Pathologie; Crassostrea gigas.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3248.pdf

Note de synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Avril-Mai 1996
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile; Le Deuff, Rose-marie; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade dans le cadre de l'étude du virus de type herpès et de la mise au point de méthodes de diagnostic fait suite à la précédente note du 16 avril 1996. Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont donnés et commentés en sept points. 1- Analyses Pays de Loire 2 - Bilan des analyses réalisées dans le cadre du protocole IFREMER BOUIN/IFREMER ARGENTON/SODABO 3 - Bilan global des analyses Pays de Loire et Bouin-Argenton-SODABO 4 - Analyses effectuées en PCR pour la recherche de virus de type herpès sur des lots prélevés en 1996, dans le...
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15499/12867.pdf

Insights into the antiviral functions of the RNAi machinery in penaeid shrimp
Provedor de dados: 5
Autores: Labreuche, Yannick; Warr, Gregory W.
Over the last decade, RNA interference pathways have emerged in eukaryotes as critical regulators of many diverse biological functions including, among others, transcriptional gene regulation, post-transcriptional gene silencing, heterochromatin remodelling, suppression of transposon activity, and antiviral defences. Although this gene silencing process has been reported to be relatively well conserved in species of different phyla, there are important discrepancies between plants, invertebrates and mammals. In penaeid shrimp, the existence of an intact and functional RNAi machinery is supported by a rapidly growing body of evidence. However, the extent to which this process participates to the host immune responses remains poorly defined in this non-model...
Tipo: Text
Palavras-chave: Shrimp; Innate immune response; In vertebrate immunity; RNA interference; Virus.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00119/23063/20899.pdf

Shrimp Taura syndrome virus: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses
Provedor de dados: 5
Autores: Mari, Jocelyne; Poulos, Bonnie; Lightner, Donald; Bonami, Jean-robert.
The single-stranded genomic RNA of Taura syndrome virus (TSV) is 10205 nucleotides in length, excluding the 3' poly(A) tail, and contains two large open reading frames (ORFs) that are separated by an intergenic region of 207 nucleotides. The ORFs are flanked by a 377 nucleotide 5' untranslated region (UTR) and a 226 nucleotide 3' UTR followed by a poly(A) tail. The predicted amino acid sequence of ORF1 revealed sequence motifs characteristic of a helicase, a protease and an RNA-dependent RNA polymerase, similar to the non-structural proteins of several plant and animal RNA viruses. In addition, a short amino acid sequence located in the N-terminal region of ORF1 presented a significant similarity with a baculovirus IAP repeat (BIR) domain of inhibitor of...
Tipo: Text
Palavras-chave: Genomic characterization; Taura syndrome; Virus; Shrimp disease; Penaeidae.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/2002/publication-715.pdf

Situation of wild beds of Crassostrea gigas regarding OsHV-1 µvar: spatiotemporal description of contamination in Pacific oyster wild beds in Marennes Oleron Bay
Provedor de dados: 5
Autores: Pepin, Jean-francois; Soletchnik, Patrick; Lupo, Coralie; Geairon, Philippe; Seugnet, Jean-luc; Robert, Stephane; Bernard, Ismal; Le Moine, Olivier.
This study focuses on the situation of wild oyster beds of Crassostrea gigas against OsHV-1 μvar and presents a spatio-temporal description of the OsHV-1 contamination in wild oyster beds in the bay of Marennes-Oléron from a range of sampling based on a hydrodynamic model and analyzes conducted in 2010. The objectives were to describe wild populations of pacific oysters in Marennes Oleron bay; to characterize these populations regarding OsHV-1 prevalence for spat and adults in connection with the isolation level from reared stocks and the sampling periods.
Tipo: Text
Palavras-chave: Virus; OsHV-1; Contamination; Oysters; Crassostrea gigas; Spatiotemporal description; Marennes Oleron Bay.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00105/21673/19251.pdf

Viruses infecting bivalve molluscs
Provedor de dados: 5
Autores: Renault, Tristan; Novoa, Beatriz.
Bivalve molluscs are filter feeders and as a consequence they may bioaccumulate in their tissues viruses that infect humans and higher vertebrates. However, there have also been described mortalities of bivalve molluscs associated with viruses belonging to different families. Mass mortalities of adult Portuguese oysters, Crassostrea angulata, among French livestocks (between 1967 and 1973) were associated with irido-like virus infections. Herpesviruses were reported in the eastern oyster, Pacific oyster and European flat oyster and lately in scallops and clams. Disseminated neoplasia, a proliferative cell disorder of the circulatory system of bivalves, although of uncertain aetiology, has been suggested to be caused by retroviral infections. Other viruses...
Tipo: Text
Palavras-chave: Infection; Virus; Molluscs; Bivalves.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-2910.pdf

Differential Mortality and High Viral Load in Naive Pacific Oyster Families Exposed to OsHV-1 Suggests Tolerance Rather than Resistance to Infection
Provedor de dados: 5
Autores: Agnew, M. Victoria; Friedman, Carolyn S.; Langdon, Christopher; Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Degremont, Lionel; Dhar, Arun K.; Kirkland, Peter; Dumbauld, Brett; Burge, Colleen A.
Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant, which is similar to the OsHV-1 reference, found in Tomales Bay, CA. The goals of this study were to investigate the resistance of C. gigas juveniles produced by the...
Tipo: Text
Palavras-chave: OsHV-1; Mu vars; Oyster; Virus; Tolerance; Virulence; QPCR.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00665/77739/79862.pdf

Herpesvirus observé chez les huîtres - Résultats août 1995/avril 1996
Provedor de dados: 5
Autores: Renault, Tristan.
Les travaux concernant le virus de type herpès, observé chez les huîtres, ont été poursuivis en 1995-1996 et ont ainsi permis d'aboutir à la mise au point d'un protocole de purification du virus, malgré l'ensemble des difficultés rencontrées (impossibilité de reproduire le virus sur lignées cellulaires, dépendance vis à vis des professionnels pour l'approvisionnement en matériel infecté et difficulté de planifier les expériences). De ce fait, le point de blocage que représentait l'obtention de particules virales en nombre suffisant pour le développement d'outils de diagnostic fiables et sensibles a été levé.
Tipo: Text
Palavras-chave: Herpès virus; Pathologie; Virus; Mortalité; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00050/16153/13636.pdf

Foodborne viruses: Detection, risk assessment, and control options in food processing
Provedor de dados: 5
Autores: Bosch, Albert; Gkogka, Elissavet; Le Guyader, Soizick; Loisy-hamon, Fabienne; Lee, Alvin; Van Lieshout, Lilou; Marthi, Balkumar; Myrmel, Mette; Sansom, Annette; Schultz, Anna Charlotte; Winkler, Anett; Zuber, Sophie; Phister, Trevor.
In a recent report by risk assessment experts on the identification of food safety priorities using the Delphi technique, foodborne viruses were recognized among the top rated food safety priorities and have become a greater concern to the food industry over the past few years. Food safety experts agreed that control measures for viruses throughout the food chain are required. However, much still needs to be understood with regard to the effectiveness of these controls and how to properly validate their performance, whether it is personal hygiene of food handlers or the effects of processing of at risk foods or the interpretation and action required on positive virus test result. This manuscript provides a description of foodborne viruses and their...
Tipo: Text
Palavras-chave: Virus; Detection; Risk assessment; Food; Processing technologies.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00443/55507/57057.pdf

Programme Pathologie - Rapport scientifique Année 1994 - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 94 RPC R 62
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno; Maffart, Patricia; Haffner, Philippe; Berthe, Franck; Thuillier, Benoit.
Le but de ce programme pathologie est de recherher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassastrea gigas, afin d'identifier d'éventuels agents causant des mortalité, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socioéconomique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la pathologie....
Tipo: Text
Palavras-chave: Pathologie; Agent pathogène; Infection; Virus; Herpèsvirus; Purification; Huître creuse; Crassostrea gigas.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00044/15502/12874.pdf

Infections virales chez les huîtres Crassostrea angulata Lmk. et Crassostrea gigas Th.
Provedor de dados: 5
Autores: Comps, Michel; Duthoit, Jean-louis.
Two viral infections associated to some epizooties have been observed in Crassostrea angulata Lmk. The first one concerns the Gill disease in which the viral damages go hand in hand with a cellular hypertrophy, while, in the second one, the contaminated cells are associated with a deterioration of the connective tissue. These branchial necrosis associated to a virus have been observed again recently in some Portuguese oysters from the natural deposits of the Cadix (Spain) area. Moreover, the study of the summer mortalities (July 1977) in the oyster Crassostrea gigas Th. demonstrated the presence in this species of a virus of the same type as the one associated to the massive mortalities of the Portuguese oyster. In both cases, the viral morphogenesis takes...
Tipo: Text
Palavras-chave: Oyster; Crassostrea gigas; Crassostrea angulata; Pathology; Virus; Huître; Crassostrea gigas; Crassostrea angulata; Pathologie; Virologie.
Ano: 1979
URL: http://archimer.ifremer.fr/doc/1979/publication-5922.PDF

REPAMO - REseau de PAtologie des MOlusques : Bilan des examens zoosanitaires réalisés en 1998
Provedor de dados: 5
Autores: Pichot, Yves.
Tipo: Text
Palavras-chave: Virus; Parasitology; Monitoring; Bivalves; Molluscs.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/rapport-1600.pdf

Lack of evidence for Litopenaeus vannamei Toll receptor (lToll) involvement in activation of sequence-independent antiviral immunity in shrimp
Provedor de dados: 5
Autores: Labreuche, Yannick; O'Leary, Nuala A.; De La Vega, Enrique; Veloso, Artur; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.
injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection...
Tipo: Text
Palavras-chave: Shrimp; Virus; RNA interference; Invertebrate immunity; Innate immune response; Toll like receptor; DSRNA.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6428.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 98 RPC R 7 - Programme Pathologie - Rapport Final année 1998
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf

Fecal contamination in coastal areas: An engineering approach
Provedor de dados: 5
Autores: Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick.
Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These...
Tipo: Text
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf

Etat des connaissances sur la pathologie chez les mollusques bivalves
Provedor de dados: 5
Autores: Fouche, Dominique.
A short description of the disease causing organisms affecting bivalves is presented in this document, including the diagnosis, the oustanding research and bivalve husbandry. Mollusc populations may be safe from disease epizootics if an early prevention of disease is coupled with a strict regulatory system on transfer (according to what happens in agriculture). The species to culture may be thoroughly selected and risky populations moved. Imported stocks are strictly controlled. The researchcs's target is to improve the diagnosis tools and try to find resistant mollusc strains. Epidemiological surveys are also necessary to control diseases.
Tipo: Text
Palavras-chave: Transfer; Husbandry; Prophylatics; Bivalve; Protozoan; Virus; Bacteria; Metazoan; Transfert; Zootechnie; Prophylaxie; Bivalve; Protozoaire; Virus; Bactérie; Métazoaire.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/rapport-1923.pdf

Etude de l'herpès virus de l'huître. Fonds d'Aide à la Recherche et à l'Innovation. Décision d'Aide à la Recherche n° 95-07-01 (Rapport intermédiaire) 1997
Provedor de dados: 5
Autores: Renault, Tristan.
Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années...
Tipo: Text
Palavras-chave: Virus; Herpès virus; Crassostrea gigas; Pathogènes; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15448/12821.pdf

Définition et test de nouvelles amorces pour la détection en PCR du virus de type herpès infectant les huîtres
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile.
La collaboration, entamée en 1996 avec A. Davison du Medical Research Council de Glasgow, a permis d'aboutir à l'obtention d'informations sur la équence de fragments clonés d'ADN de virus de type herpès infectant les huîtres.
Tipo: Text
Palavras-chave: Détection PCR; Virologie; Virus; Herpès; Huîtres creuses; Crassostrea gigas; Techniques.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00199/31048/29465.pdf

Synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Juin-Septembre 1996
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type . herpès et de la mise au point des méthodes de diagnostic fait suite à la précédente note (fin mai 1996). Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont données et commentés en quatre points. Cette étude est soutenue par la Région Poitou-Charente et la Région Pays de Loire.
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Infection; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15503/12875.pdf

Mortalités massives en élevage larvaire de Lates Calcarifer ( Mise en évidence d'un virus neurotrope )
Provedor de dados: 5
Autores: Renault, Tristan; Thouard, Emmanuel; Weppe, Maurice.
Since 1986 high mortality of hatchery-reared sea bass, Lates Calcarifer, larvae appeared in Ifremer's Pacifie Oceanological Center (Tahiti). Light and electron microscopy show vacuolation and cells with cytoplasmic inclusion bodies in the brain, the cord and the eye relina of affected larvae. Numerous non-enveloped particles identified as viral units, icosahedral in morphology and measuring about 25 nm in diameter were found in the cytoplasm of the degenerating cells. In affected larvae, these particles were isolated by density grandient centrifugations. In vitro tests on cell lines and in vivo test on fish are performed with samples of diseased larvae. And immunisation of mice are made with purified immunoglobulins of Lates calcarifer.
Tipo: Text
Palavras-chave: Aquaculture; Élevage larvaire; Lates calcarifer; Pathologie; Virus; Spongiose; Aquaculture; Hatchery; Lates calcarifer; Pathology; Virus; Spongiosis.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00142/25306/23378.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 97 RPC R 58 - Programme Pathologie - Rapport Scientifique année 1997
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf

Programme Herpèsvirus. Synthèse des travaux réalisés au Laboratoire IFREMER de La Tremblade - Mars 1996 - Février 1997
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Ce rapport représente la synthèse des travaux réalisés de mars 1996 à février 1997 au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type herpès observé chez les huîtres et de la mise au point de méthodes de diagnostic. La majorité des travaux rapportés ici de façon complète, a déjà fait l'objet de comptes rendus intermédiaires.
Tipo: Text
Palavras-chave: Pathologie; Herpèsvirus; Herpesviridae; Huître creuse; Crassostrea gigas; Huître plate; Ostrea edulis; Virus; Détection; ¨Protocole; ADN; PCR.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00165/27663/25838.pdf

Effects of experimental warming on small phytoplankton, bacteria and viruses in autumn in the Mediterranean coastal Thau Lagoon
Provedor de dados: 5
Autores: Courboulès, Justine; Vidussi, Francesca; Soulié, Tanguy; Mas, Sébastien; Pecqueur, David; Mostajir, Behzad.
To investigate the responses of a natural microbial plankton community of coastal Mediterranean waters to warming, which are still poorly known, an in situ mesocosm experiment was carried out in Thau Lagoon during autumn 2018. Several microorganisms, including virio-, bacterio-, and phytoplankton &lt; 10 µm in size, were monitored daily and analysed using flow cytometry for 19 consecutive days in six mesocosms. Three mesocosms (control) had the same natural water temperature as the lagoon, and the other three were warmed by + 3 °C in relation to the control temperature. The cytometric analyses revealed an unexpected community dominated by picophytoplanktonic cells, including Prochlorococcus-like and Picochlorum-like cells, which had not previously been...
Tipo: Text
Palavras-chave: Pico- and nanophytoplankton; Bacterioplankton; Virus; Warming; In situ mesocosm; Thau lagoon.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00688/79979/82933.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par des virus. Sous-région marine Mers celtiques. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Pommepuy, Monique.
Tipo: Text
Palavras-chave: Mers celtiques; Coquillages; Virus; Risques; Sources de contamination; Impacts.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00329/43978/43550.pdf

Recherche sur la paralysie virale du crustace decapode Macropipus depurator L.
Provedor de dados: 5
Autores: Bonami, Jean-robert.
The trembling of legs followed by increasing paralysis and eventual death, were recorded on specimens of the above sp from Sete region. EM studies of tissue showed paraspherical bodies measuring approx 61 nm with a size range of 58-65 nm. By experimentally infecting specimens with a purified viral suspension the following pathogenesis was observed: in greater than 60 per cent of the specimens, trembling of the legs began within 6 days, growing very pronounced on the 8th day, leading to a general paralysis and 9 days after the infection a mortality rate of 70-80 per cent was recorded. The following characteristics of the virus are noted: its cytoplasmic localisation, paraspherical form, size ranging from 60-70 nm and the absence of an envelope. Considering...
Tipo: Text
Palavras-chave: Paralysis; Virus; Decapoda.
Ano: 1973
URL: http://archimer.ifremer.fr/doc/1973/publication-2151.pdf

In situ structuring of virioplankton through bacterial exoenzymatic activity: interaction with phytoplankton
Provedor de dados: 5
Autores: Ory, Pascaline; Palesse, S.; Delmas, Daniel; Montanie, Helene.
The abundance and composition of microbial communities were investigated during 2007 in Marennes Oleron Bay (France) in order to characterize the biological relationships governing the planktonic food web. Compared to the results of previous years, there was a lower abundance and a lower magnitude of variations in autotrophic and heterotrophic microbial entities in 2007. Using a Spearman rank correlation analysis, a significant link for biological abundance was only noted between bacteria and ciliates. However, an interesting relationship was highlighted between chlorophyll a and bacterial aminopeptidase and beta-glucosidase exoenzymatic activities. Two characteristic periods of virus-bacteria uncoupling were observed in spring and autumn 2007. Using...
Tipo: Text
Palavras-chave: Virus; Bacteria; Phytoplankton; Diversity; Aminopeptidase.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00050/16099/13571.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par des virus. Sous-région marine Golfe de Gascogne. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Pommepuy, Monique.
Tipo: Text
Palavras-chave: Golfe de Gascogne; Coquillages; Virus; Risques; Sources de contamination; Impacts.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00329/44062/43633.pdf

Epidémiologie analytique et descriptive des agents pathogènes, étude de la diversité des virus de type herpès (action 1). Etude des relations hôtes/agents pathogènes, pathogénèse des infections à virus de type herpèsvirus (action 2) - Contrat Région Poitou-Charentes 2000-2006
Provedor de dados: 5
Autores: Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno.
Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés.
Tipo: Text
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf

Microbial interactions in marine water amended by eroded benthic biofilm: A case study from an intertidal mudflat
Provedor de dados: 5
Autores: Montanie, Helene; Ory, Pascaline; Orvain, Francis; Delmas, Daniel; Dupuy, Christine; Hartmann, Hans J.
In shallow macrotidal ecosystems with large intertidal mudflats, the sediment-water coupling plays a crucial role in structuring the pelagic microbial food web functioning, since inorganic and organic matter and microbial components (viruses and microbes) of the microphytobenthic biofilm can be suspended toward the water column. Two experimental bioassays were conducted in March and July 2008 to investigate the importance of biofilm input for the pelagic Microbial and Viral Loops. Pelagic inocula (&lt; 0.6 μ- and &lt; 10 μ filtrates) were diluted either with &lt; 30 kDa-ultrafiltered seawater or with this ultrafiltrate enriched with the respective size-fractionated benthic biofilm or with &lt; 30 kDa-benthic compounds (BC). The kinetics of heterotrophic...
Tipo: Text
Palavras-chave: Mudflats; Virus; Bacteria; HNF; Coastal water.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00166/27691/25883.pdf

Infecciones de Transmisión Sexual en Semen: El Hombre como Vector de Transmisión
Provedor de dados: 77
Autores: Viscarra A,Tamara; Brebi M,Priscilla; Andana V,Alejandra; Sánchez G,Raúl.
En los últimos años el estudio de las infecciones de transmisión sexual ha cobrado gran importancia debido principalmente al incremento de estas en parejas heterosexuales y hombres que tienen sexo con hombres. En mujeres existe mucha información de epidemiología y patogénesis de estas infecciones, sin embargo, en hombres la información es muy escasa debido a que la mayoría no presenta sintomatología. En los últimos años se ha evidenciado un creciente interés en el estudio del semen como vía de transmisión, debido principalmente a la afinidad de algunos patógenos con los espermatozoides. Dentro de los principales microorganismos infectantes en semen se encuentran Chlamydia trachomatis, Neisseria gonorrhoeae, Mollicutes, Virus de la Inmunodeficiencia Humana...
Tipo: Journal article
Palavras-chave: Infecciones de transmisión sexual; Semen; Virus; Bacterias; Protozoos.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100041

Desarrollo de una técnica de inmunodifusión para la detección del virus Sacbrood en abejas
Provedor de dados: 78
Autores: Albo,GN.; Kuzmanich,R.; Reynaldi,FJ.; Picotto,LD.; Sguazza,GH.
La apicultura es una de las actividades agrícolas más importantes de la República Argentina. En los últimos años se ha observado, a nivel mundial, una marcada disminución en el número de abejas. La mortalidad de las abejas, puede atribuirse a la interacción de factores ambientales, al manejo de las colonias o a la acción de diversos microorganismos, entre ellos los virus. Teniendo en cuenta que en la Argentina en este momento solamente se cuenta con técnicas de diagnostico moleculares para la detección de virus, el objetivo de este trabajo fue desarrollar un test de inmunodifusión que permita la detección del Virus de la Cría Ensacada de forma más sencilla y económica. Para ello una cepa autóctona del virus Virus de la Cría Ensacada fue utilizada para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abejas melíferas; Virus; Cría ensacada; Inmunodifusión.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982016000200009

Virus de Distemper Canino: detección molecular de diferentes aislamientos provenientes de perros de la provincia de santa fe, argentina, entre los años 2000 y 2010
Provedor de dados: 78
Autores: Pinotti,M.; Gollan,A; Canavesio,M.; Passeggi,C.; Larrateguy,M.V.; Paz,M.E.; Formentini,E.
En la ciudad de Santa Fe, Argentina, durante los años 2000 y 2010, se aislaron 13 cepas autóctonas del virus Distemper Canino. Las muestras se tomaron de caninos con signos clínicos de la enfermedad y consistieron en hisopados nasales, oculares, faríngeos (tonsilas) y conjuntivales. Los aislamientos se realizaron en células MDCK, las cuales mostraron efecto citopatogénico característico. Los virus provenientes de la cosecha, se detectaron por RT-PCR de una región que codifica la nucleoproteína NP asociada al RNA (posición 769 a la 1055 del genoma) obteniéndose en todos los casos un producto de 287 pb comparable con el obtenido con cepas vacunales (cepas Ondersterpoort y Lederle), lo que indica presencia viral en todas las muestras procesadas. Las cepas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virus; Distemper canino; Estudio molecula; RT-PCR.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982016000200007

Outbreaks of Ebola virus disease in Africa: the beginnings of a tragic saga
Provedor de dados: 80
Autores: Chippaux,Jean-Philippe.
The tremendous outbreak of Ebola virus disease occurring in West Africa since the end of 2013 surprises by its remoteness from previous epidemics and dramatic extent. This review aims to describe the 27 manifestations of Ebola virus that arose after its discovery in 1976. It provides an update on research on the ecology of Ebola viruses, modes of contamination and human transmission of the disease that are mainly linked to close contact with an infected animal or a patient suffering from the disease. The recommendations to contain the epidemic and challenges to achieve it are reminded.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ebola; Outbreak; Virus; Hemorrhagic fever; Africa.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200209

Microbial control of arthropod pests of tropical tree fruits
Provedor de dados: 82
Autores: Dolinski,Claudia; Lacey,Lawrence A.
A multitude of insects and mites attack fruit crops throughout the tropics. The traditional method for controlling most of these pests is the application of chemical pesticides. Growing concern on the negative environmental effects has encouraged the development of alternatives. Inundatively and inoculatively applied microbial control agents (virus, bacteria, fungi, and entomopathogenic nematodes) have been developed as alternative control methods of a wide variety of arthropods including tropical fruit pests. The majority of the research and applications in tropical fruit agroecosystems has been conducted in citrus, banana, coconut, and mango. Successful microbial control initiatives of citrus pests and mites have been reported. Microbial control of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Virus; Fungus; Nematode.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000200001

The Role of Carbohydrates in Infection Strategies of Enteric Pathogens
Provedor de dados: 19
Autores: Kato, Kentaro; Ishiwa, Akiko; 加藤, 健太郎.
Enteric pathogens cause considerable public health concerns worldwide including tropical regions. Here, we review the roles of carbohydrates in the infection strategies of various enteric pathogens including viruses, bacteria and protozoa, which infect the epithelial lining of the human and animal intestine. At host cell entry, enteric viruses, including norovirus, recognize mainly histo-blood group antigens. At the initial step of bacterial infections, carbohydrates also function as receptors for attachment. Here, we describe the function of carbohydrates in infection by Salmonella enterica and several bacterial species that produce a variety of fimbrial adhesions. During invasion by enteropathogenic protozoa, apicomplexan parasites utilize sialic acids...
Palavras-chave: Bacteria; Carbohydrate; Enteric pathogen; Infection; Protozoa; Virus.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4299

Sequence similarity between the viral cp gene and the transgene in transgenic papayas
Provedor de dados: 86
Autores: Souza Júnior,Manoel Teixeira; Gonsalves,Dennis.
The Papaya ringspot virus (PRSV) coat protein transgene present in 'Rainbow' and 'SunUp' papayas disclose high sequence similarity (&gt;89%) to the cp gene from PRSV BR and TH. Despite this, both isolates are able to break down the resistance in 'Rainbow', while only the latter is able to do so in 'SunUp'. The objective of this work was to evaluate the degree of sequence similarity between the cp gene in the challenge isolate and the cp transgene in transgenic papayas resistant to PRSV. The production of a hybrid virus containing the genome backbone of PRSV HA up to the Apa I site in the NIb gene, and downstream from there, the sequence of PRSV TH was undertaken. This hybrid virus, PRSV HA/TH, was obtained and used to challenge 'Rainbow', 'SunUp', and an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carica papaya; Potyvirus; Resistance; Breeding; Virus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000500009

Occurrence of Aceria tosichella in Brazil
Provedor de dados: 86
Autores: Pereira,Paulo Roberto Valle da Silva; Navia,Denise; Salvadori,José Roberto; Lau,Douglas.
The objective of this work was to evaluate the occurrence of Aceria tosichella Keifer (Prostigmata: Eriophyidae), the wheat curl mite, in Rio Grande do Sul, Brazil. Samples to detect A. tosichella specimens were collected in wheat, corn, oat crops and potential host grasses in 46 localities, in October 2006, August and October 2007. Samples of wheat were also collected in experimental greenhouses at Embrapa Trigo, Passo Fundo, RS, Brazil. A. tosichella specimens were found in wheat samples from Passo Fundo, Palmeira das Missões, São Luís Gonzaga, and Santo Antônio das Missões, RS, Brazil. Symptoms due to A. tosichella infestations were observed only in greenhouse conditions. This is the first report of A. tosichella in Brazil and the second in South America.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eriophyoidea; South America; Acari; Cereal; Quarantine; Virus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500015

Turnip mosaic virus infecting kale plants in Ordu, Turkey
Provedor de dados: 85
Autores: Sevik,MA.
Brassica oleracea var. acephala L. (kale) is widely grown in the Black Sea Region of Turkey. Kale growing has not been common in the other regions of Turkey. A number of diseases can seriously afect Brassica crop production. Field surveys were done to determine the occurrence of viruses in kale-growing areas in Ordu in 2013-2014. Leaf samples were collected from kale plants and tested for the presence of Turnip mosaic virus (TuMV), Cauliflower mosaic virus (CaMV), Turnip yellow mosaic virus (TYMV), and Cucumber mosaic virus (CMV) by DAS-ELISA and bioassays. Result of serological and biological tests showed that 7.7% of these samples were infected with TuMV. However, CaMV, CMV, and TYMV were not detected in any of the tested kale plants. The occurrence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica; Kale; Survey; Disease; Virus.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200009

Primer reporte de Tobacco mosaic virus (TMV) y Poinsettia mosaic virus (PnMV) en nochebuena de sol (Euphorbia pulcherrima Willd. Ex Klotzch) en México
Provedor de dados: 85
Autores: Ocampo Ocampo,T; Ochoa Martínez,DL; Ramírez Rojas,S; Valdovinos Ponce,G; Nava Díaz,C.
El Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), a través del Campo Experimental "Zacatepec" en el estado Morelos, inició un programa de mejoramiento genético de plantas silvestres y semicultivadas de nochebuena ("nochebuena de sol"). Dicho programa requiere de la generación de una base de datos fitosanitarios con tolerancia a los principales patógenos. México no cuenta con información sobre las enfermedades causadas por virus en la nochebuena de sol, por lo que el objetivo de la presente investigación fue generar datos preliminares sobre los virus asociados a estos materiales. Con base en los resultados obtenidos en las técnicas DAS-ELISA y RT-PCR, se reportan por primera vez en México los virus Tobacco mosaic virus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nochebuena silvestre; Nochebuena semicultivada; Virus; DAS-ELISA; RT-PCR.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572013000200011

EVA: EQUINE VIRAL ARTERITIS AND THE U.S. HORSE INDUSTRY
Provedor de dados: 31
Autores: Garber,   Lindsey.
The National Animal Health Monitoring System (NAHMS) is sponsored by the USDA:APHIS:Veterinary Services (VS). The NAHMS Equine '98 Study was designed to provide information about the nation's equine population for education and research purposes. Equine viral arteritis population for education and research purposes. Equine viral arteritis (EVA) is an infectious disease caused by the equine arteritis virus (EAV). It can cause abortion in pregnant mares and death in young foals. Breeding stallions may become permanent carriers of the virus. Financial losses may include those due to abortion and/or disease and death in very young foals, reduced demand to breed and/or decreased commercial value of persistently infected stallions, and denied export market for...
Tipo: Report
Palavras-chave: NAHMS; Horse; Equine; Equid; Pony; Stallion; Mare; Foal; Breeding; Equine viral arteritis; EVA; Abortion; Virus; Export; EAV; Equine arteritis virus; Racing; Vaccination; Titer; Breed; Standardbred; Livestock Production/Industries.
Ano: 2000
URL: http://purl.umn.edu/32749

NEW OCCURANCE OF AVIAN ENCEPHALOMYELITIS IN BROILER - IS THIS AN EMERGING DISEASE?
Provedor de dados: 89
Autores: de,Freitas ES; Back,A.
ABSTRACTAvian encephalomyelitis is caused by an Hepatovirus and primarily affects chickens. Chickens of all ages are susceptible to the virus, but the nervous symptoms are manifested only in young chicks, between one to five weeks of age. During the last thirty years, avian encephalomyelitis appeared to be well controlled by breeder vaccination. However, the increase of the number of cases is causing concern in the poultry industry. In the present study, we performed a retrospective analysis of the cases presenting histological lesions compatible with avian encephalomyelitis in broilers. The evaluated cases affected broilers from one to 35 days old from the southern region of Brazil. Only cases with compatible microscopic lesions and associated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reemerging; Disease; Young chickens; Virus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000300399

Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil
Provedor de dados: 89
Autores: Pinto,LB; Ometto,T; Araújo,J; Thomazelli,LM; Seixas,MM; Barbosa,CM; Ramos,DGS; Melo,ALT; Pinho,JB; Durigon,EL; Aguiar,DM.
ABSTRACT The Pantanal is the world's largest wetland biome with a seasonal flood pulse that attracts a great diversity of birds, many of which are migratory. Birds can be natural reservoirs Influenza A, West Nile and Newcastle Disease viruses. However, the occurrence of carriers for these viruses in the Pantanal was not verified yet. The present study evaluated the occurrence of natural infection by Influenza A, WN and ND virus of birds in the municipality of Poconé, a subregion of the Pantanal in the state of Mato Grosso, Brazil. A total of 76 birds belonging to 11 orders and 20 families were captured using mist nets. The most representative order was Passeriformes, followed by the other nine orders, which included Columbiformes, Psittaciformes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pantanal birds; Infectious Diseases; RT-PCR; QRT-PCR; Virus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200291

Crimean-Congo haemorrhagic fever in ostriches: could it be a public health risk?
Provedor de dados: 195
Autores: Doretto Júnior, Luciano.
Crimean-Congo haemorrhagic fever is a zoonose caused by a virus of nairovirus genera, which maybe transmitted by ixodides ticks. The ostriches among other domestic and wild birds species are refractory to CCHF, but in humans the disease have shown to be lethal in 30% of the cases. The disease is disserninated in A ia, Africa and east of Europe. In the past few year in South Africa – The first country to produce and export ostriches, many cases of such disease were reported in people who work in slaughterhouses, as weU a people who deal with ostriches and/or diagnostic laboratory technician.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ostrich; Crimean-Congo haemorrhagic fever; Avian pathology; Virus; Nairovirus; Ticks avestruz; Febre hemorrágica crimean-congo; Patologia aviária; Vírus; Nairovirus; Carrapatos.
Ano: 1999
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3377

Detección de infecciones mixtas en genotipos de caña de azúcar en Cuba
Provedor de dados: 100
Autores: Zardón,María A; Gallo,Araíz; Mesa,José M; Arencibia,Ariel; Zamora,Loidy; Sautié,Miguel; Casas,Mario A; La O,María L.
Los síntomas de amarilleamiento y clorosis presentan alta incidencia en el Banco de Germoplasma de la Caña de Azúcar de Cuba. El objetivo de este trabajo fue determinar su asociación con fitoplasmas, Xanthomonas albilineans (Ashby) Dowson, los virus del mosaico y de la hoja amarilla. Para ello se seleccionaron del Banco de Germoplasma aquellos genotipos que manifestaron síntomas de amarilleamiento y clorosis de forma sostenida durante el ciclo de vida del cultivo. Se diagnosticaron ambos virus empleando la técnica Reverso Transcriptasa-Reacción en Cadena de la Polimerasa (RT-PCR), mientras que X. albilineans y fitoplasmas fueron estudiados por PCR anidada. Del total de muestras, ocho resultaron positivas al virus de la hoja amarilla, tres al mosaico, ocho...
Tipo: Journal article
Palavras-chave: Saccharum spp.; Virus; Fitoplasmas; Xanthomonas albilineans.
Ano: 2012
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522012000200002

Molecular-assisted selection for resistance to cassava mosaic disease in Manihot esculenta Crantz
Provedor de dados: 63
Autores: Carmo,Cátia Dias do; Silva,Maiane Suzarte da; Oliveira,Gilmara Alvarenga Fachardo; Oliveira,Eder Jorge de.
The geminivirus complex known as cassava mosaic disease (CMD) is one of the most devastating viruses for cassava (Manihot esculenta Crantz). The aim of this study was to use molecular-assisted selection (MAS) to identify CMD-resistant accessions and ascertain promising crosses with elite Brazilian varieties. One thousand two hundred twenty-four accessions were genotyped using five molecular markers (NS169, NS158, SSRY028, SSRY040 and RME1) that were associated with resistance to CMD, along with 402 SNPs (single-nucleotide polymorphism). The promising crosses were identified using a discriminant analysis of main component (DAPC), and the matrix of genomic relationship was estimated with SNP markers. The CMD1 gene, previously described in M. glaziovii, was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Breeding; Disease; Virus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000600520

Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
Provedor de dados: 102
Autores: Souza Júnior,Manoel T.; Nickel,Osmar; Gonsalves,Dennis.
Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carica papaya; Potyvirus; Resistance; Breeding; Virus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400004

Evaluación agronómica de accesiones de Capsicum del banco de germoplasma de la Universidad Nacional de Colombia Sede Palmira
Provedor de dados: 109
Autores: Pardey Rodríguez,Catherine; García Dávila,Mario Augusto; Vallejo Cabrera,Franco Alirio.
En un diseño de bloques incompletos 10 x 10 con tres repeticiones se estudiaron descriptores cuantitativos relacionados con producción por planta, contenido de capsaicina y presencia de virus en 100 accesiones de Capsicum pertenecientes a la Universidad Nacional de Colombia. Las diferencias entre las accesiones fueron explicadas por el contenido de capsaicina, características asociadas con la producción por planta y características del fruto. Se encontró correlación inversa entre el contenido de capsaicina y producción; número de frutos por planta con peso de fruto. La dispersión de las accesiones en el plano cartesiano situó las variedades comerciales Cayenne, Tabasco y Habanero, en función de producción, peso y cantidad de frutos, en posiciones...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pimentón; Ají; Producción; Capsaicina; Virus.
Ano: 2009
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122009000100004

Herencia de la resistencia al virus del mosaico deformante del pimentón PepDMV en Capsicum
Provedor de dados: 109
Autores: García Dávila,Mario Augusto; Pardey Rodríguez,Catherine.
En busca de determinar el modo de herencia de la resistencia en tres materiales resistentes, se hicieron cruzamientos hacia tres líneas susceptibles. Se siguió el método del retrocruzamiento propuesto por Warner (1952) fundamentado en el modelo matemático de Fisher Immer y Tedin (1932) descrito con detalle por Mater (1949) y Jenkins (1982, 1984) en el cual se descompone la varianza genética en tres componentes: aditiva, dominancia y epistática. El modelo del retrocruzamiento incluye los dos parentales, el hibrido de la primera generación y la autofecunfación de la F1 para formar la población F2 y las retrocruzas hacia ambos padres. Las poblaciones fueron evaluadas a resistencia al virus del mosaico del pimentón PepDMV en condiciones de invernadero. Los...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanaceae; Ají; Virus; Resistencia varietal; Capsicum annuum; C. frutescens; C. chínense.
Ano: 2009
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122009000400013

GARRAPATAS DURAS (ACARI: IXODIDAE) DE COLOMBIA, UNA REVISIÓN A SU CONOCIMIENTO EN EL PAÍS
Provedor de dados: 37
Autores: ACEVEDO-GUTIÉRREZ,Leidy Yoana; PATERNINA,Luis Enrique; PÉREZ-PÉREZ,Juan Camilo; LONDOÑO,Andrés Felipe; LÓPEZ,Gustavo; RODAS,Juan David.
RESUMEN Las garrapatas son artrópodos de gran impacto sobre la economía pecuaria y la salud humana debido a las pérdidas generadas en la producción, y a su capacidad de transmitir microorganismos. Pese a la evidencia de circulación de una gran diversidad de garrapatas en Colombia, no se cuenta con compilaciones actualizadas de la variedad de especies presentes, por lo cual los autores presentan una revisión de literatura sobre las garrapatas de la familia Ixodidae reportadas en Colombia incluyendo su distribución geográfica, sus hospederos y los microorganismos reportados en aquellas especies registradas en este país. En la recopilación se evidenció la presencia en Colombia de diferentes especies de garrapatas de los géneros Amblyomma, Ixodes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artrópodos vectores; Artrópodos hematófagos; Bacteria; Distribución espacial; Infestación; Virus.
Ano: 2020
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000100126

DESARROLLO DE UN POXVIRUS RECOMBINANTE QUE EXPRESA LA GLICOPROTEÍNA D DEL HERPESVIRUS BOVINO-1
Provedor de dados: 37
Autores: RUIZ SÁENZ,JULIÁN; OSORIO,JORGE E; VERA,VÍCTOR J.
El herpesvirus bovino-1 (BHV-1) es un virus de genoma DNA perteneciente a la familia Herpesviridae, el cual afecta al bovino en el que provoca un amplio espectro de manifestaciones clínicas y pérdidas económicas. El principal componente inmunogénico de su envoltura es la glicoproteína D (gD), la cual ha sido caracterizada y utilizada como inmunógeno en distintos sistemas de expresión. El objetivo de este trabajo fue generar un poxvirus recombinante (Raccoonpox [RCN]) que expresara una versión truncada de la gD del BHV-1 para ser usado como inmunógeno. Para ello, se amplificó el gen que codifica para la versión truncada de la gD, la cual se clonó en el plásmido de transferencia pTK/ IRES/tpa que posee sitios de homología a la timidinakinasa del poxvirus, un...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Clonación molecular; Rinotraqueitis; Vacunas; Virus; Vectores genéticos.
Ano: 2012
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000300004

IDENTIFICACIÓN DEL VIRUS DEL MOSAICO ESTRIADO DE LA CEBADA (Barley stripe mosaic virus, BSMV) EN CEBADAS EN CHILE
Provedor de dados: 113
Autores: Herrera M.,Guido; Beratto M.,Edmundo; Andrade V.,Orlando; Madariaga V.,Mónica.
Semillas de cebadas (Hordeum vulgare L.) introducidas desde Argentina se sembraron en la zona sur de Chile. Las plantas adultas mostraron mosaico estriado en las hojas. Estos síntomas sugirieron la presencia del virus del mosaico estriado de la cebada (BSMV). Las plantas se sometieron a pruebas de transmisión mecánica, microscopía electrónica, ELISA y PCR (reacción en cadena de la polimerasa). Los resultados permitieron establecer como el agente causal de los síntomas en las plantas a BSMV. Esta es la primera identificación del virus en Chile, y como las plantas infectadas se encontraron en un lugar específico, todo el material fue eliminado mediante quema.
Tipo: Journal article
Palavras-chave: Cebada; Virus; Chile; BSMV.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072001000300003

Presencia de Diferentes Virus de Pimiento (Capsicum annuum L.) en Especies de Malezas Asociadas al Cultivo
Provedor de dados: 113
Autores: Ormeño N,Juan; Sepúlveda R,Paulina.
En prospecciones de malezas realizadas durante dos temporadas agrícolas, 30 especies anuales y 13 perennes fueron determinadas en cultivos de pimientos (Capsicum annuum L.) de los Valles de Elqui y Limarí, Región de Coquimbo (29° a 30º lat. Sur). Los muestreos fueron aleatorios dentro y fuera de los potreros, con y sin cultivo presente, en invierno y primavera. Se recolectaron 676 plantas de malezas, analizándose un total de 379 muestras. Utilizando la prueba DAS-ELISA se determinó Virus del mosaico del pepino (CMV), Virus del bronceado del tomate (TSWV), Virus del mosaico de la alfalfa (AMV), Virus Y de la papa (PVY) y Virus INSV (Impatients necrotic spot virus). Un 17% (64) de las muestras fueron positivas al menos a un virus, de las cuales 7,4% (28)...
Tipo: Journal article
Palavras-chave: Malezas; Virus; Epidemiología; Pimientos; CMV; AMV; PVY; TSWV; INSV.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072005000400001

INCIDENCIA DE PRUNUS NECROTIC RINGSPOT VIRUS (PNRSV), PRUNE DWARF VIRUS (PDV), TOMATO RINGSPOT VIRUS (ToRSV) Y PLUM POX VIRUS (PPV) EN VIVEROS DE FRUTALES DE CAROZO DE LA ZONA CENTRAL DE CHILE
Provedor de dados: 113
Autores: Herrera M.,Guido; Madariaga V.,Mónica.
La forma más eficiente de control de los virus en frutales es la utilización de material limpio en el establecimiento de los huertos. Por tanto, es de primordial importancia la limpieza de virus en plantas de viveros. A la fecha, no existen antecedentes cuantitativos de la incidencia de los diferentes virus a nivel de viveros de frutales de carozo en Chile. Esta información es básica para justificar el desarrollo de programas tendientes a la producción de plantas limpias de virus conocidos. El objetivo de esta investigación fue cuantificar la incidencia de prunus necrotic ringspot virus (PNRSV), prune dwarf virus (PDV), tomato ringspot virus (ToRSV) y plum pox virus (PPV) en viveros de frutales de carozo en la zona central de Chile. El trabajo se realizó...
Tipo: Journal article
Palavras-chave: Frutales de carozo; Virus; PNRSV; PDV; ToRSV; PPV.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072002000100004

PRESENCIA E INCIDENCIA DE VIRUS DE LA VID EN LA ZONA CENTRAL DE CHILE
Provedor de dados: 113
Autores: Herrera M.,Guido; Madariaga V.,Mónica.
Entre las temporadas de cultivo 1996 y 2000 se realizó una prospección con el propósito de establecer la incidencia de Grape Fan Leaf virus (GFLV), Grape Leaf Roll Virus (GLRV), Tomato Ringspot Virus (ToRSV) y Cherry Leaf Roll Virus (CLRV) en vides (Vitis vinifera L.), tanto en producción como en material madre para propagación. Se analizaron 16.974 muestras provenientes de 33 localidades. En cada predio se colectaron muestras siguiendo el siguiente esquema: en forma correlativa se tomó la décima hilera y dentro de la hilera se colectaron muestras, sucesivamente, de la décima planta. Todas las muestras se analizaron para cada uno de los virus indicados mediante la prueba ELISA empleando sueros policlonales (SANOFI, Francia). Los resultados mostraron que el...
Tipo: Journal article
Palavras-chave: Viñas; Uvas; Virus.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072001000400001

Ocurrencia de un tobamovirus asociado con manchas anulares amarillas en nopal tunero en México
Provedor de dados: 115
Autores: Torre-Almaraz,Rodolfo De La; Salazar-Segura,Mario; Ruiz-Medrano,Roberto.
Resumen: Se detectó un virus en cladodios de nopal tunero cultivado en la región de San Martín de las Pirámides, Estado de México, con manchas anulares de color amarillo. Los cladodios afectados desarrollan, en ataques severos, clorosis y marchitez. El virus se transmitió mecánicamente a algunas especies de plantas hospedantes indicadoras, causando lesiones locales cloróticas y después necróticas en las hojas inoculadas; sólo Nicotiana tabacum L. var. White Burley y Capsicum annuum L. desarrollaron además mosaicos tenues en hojas no inoculadas. El desarrollo de síntomas en los hospedantes alternos fue lento, pero se aceleraron a temperaturas mayores a 30 °C en invernadero. El análisis electroforético y el tratamiento con nucleasas del ARN replicativo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Opuntia amyclaea; Virus.
Ano: 2007
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952007000700763

Tomato spotted wilt virus: agente causal de la marchitez del Miguelito (Zinnia elegans Jacquin) en el Estado de Morelos, México
Provedor de dados: 115
Autores: Morales-Díaz,Ma. Valeria; Alcacio-Rangel,Salomé; De La Torre-Almaraz,Rodolfo.
Se observaron síntomas de moteado, manchas necróticas anulares, enanismo y marchitez severa en plantas de miguelito (Zinnia elegans Jacquin) (Fam: Compositae) cultivadas en viveros comerciales del Estado de Morelos, México. Por tanto, el objetivo del presente trabajo fue determinar la etiología de esta enfermedad. Se identificó al virus marchitez manchada del tomate (Tomato spotted wilt virus. TSWV) como el agente causal de la enfermedad del miguelito con base en los ensayos de transmisión mecánica en plantas indicadoras y de patogenicidad en plántulas sanas de Z. elegans, cultivadas desde semilla en el invernadero. En las plantas el virus causó los síntomas de moteado, manchas necróticas en forma de anillos concéntricos, enanismo y marchitez, idénticos a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zinnia elegans; Plantas ornamentales; TSWV; Virus.
Ano: 2008
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952008000300008

Biological and serological characteristics of Zucchini yellow mosaic virus isolated from Zucchini (Cucurbita pepo), squash (C. maxima), and pumpkin (C. moschata)
Provedor de dados: 115
Autores: Sevik,Mehmet Ali.
Abstract Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses of cucurbit crops, causing severe mosaic, blistering of leaf lamina, malformation, and knobbed fruits, and presents an important biological variability. The objective of this study was to determine the prevalence and incidence of ZYMV in major summer (Cucurbita pepo), and winter squashes (C. maxima, and C. moschata) growing areas of Turkey-Samsun. Field surveys were made in 2015-2016 cropping seasons in major cucurbit growing regions. In five regions, 225 symptomatic samples were collected. Samples from three different species of Cucurbita were tested by biological and serological method using ZYMV polyclonal antiserum. Serological test results revealed that 84...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological and serological tests; Summer squash; Virus; Winter squash; ZYMV.
Ano: 2018
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952018000701005

Viruses infecting cool season crops in the northern Turkey
Provedor de dados: 42
Autores: SEVIK,MEHMET A.
Abstract:Virus diseases of cool season vegetable crops (mainly cabbage, white and red head cabbage, broccoli, kale, radish, rocket salad, garden cress, and turnip) were surveyed in Bafra Plain, Turkey during winter 2017, and 2018. Leaf samples were collected from different species of the Brassicaceae family showing mosaic, mottling, necrotic spots, malformation, and chlorosis symptoms. These samples were tested for the presence of Cauliflower mosaic virus (CaMV), Cucumber mosaic virus (CMV), Beet western yellows virus (BWYV), Radish mosaic virus (RaMV), Turnip mosaic virus (TuMV), Turnip yellow mosaic virus (TYMV), and Turnip yellows virus (TuYV) by biological and serological methods. A total of 455 samples were collected from cole crop fields and tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassicaceae family; Cole crop; Plant disease; Occurrence; Virus.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500853

Morphometric analysis of the thymus of puppies infected with the Snyder Hill Strain of canine distemper virus
Provedor de dados: 45
Autores: Alves,C.M.; Vasconcelos,A.C.; Martins,A.S.; del Puerto,H.L.; Santos,F.G.A.; Nunes,J.E.S.; Campos,P.P.; Moro,L.
The thymic morphometry analysis was used for determining apoptosis and atrophy of the thymus of eight puppies inoculated with canine distemper virus (CDV). Three healthy dogs were used as negative controls. Sections, 5µm thick, were stained by HE and Shorr, and the latter were evaluated by morphometry. CDV nucleoprotein was detected by immunohistochemistry. Morphometric results confirmed lymphoid hypotrophy in CDV inoculated dog thymuses, more stroma, less parenchyma and higher apoptotic index/field than negative control (not inoculated) puppies. Apoptosis plays a role in the mechanism of thymus atrophy that develops in canine distemper.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puppy; Apoptosis; Thymus; Canine distemper; Immunology; Virus; Immunosuppression.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400004

Avaliação sorológica para Toxoplasma gondii pela imunofluorescência indireta e detecção do vírus da imunodeficiência felina pela nested PCR em felinos selvagens
Provedor de dados: 45
Autores: Rivetti Jr.,A.V.; Caxito,F.A.; Resende,M.; Lobato,Z.I.P.
Nineteen sera and blood samples from wild feline kept in captivity were tested for Toxoplasma gondii antibody and presence of feline immunodeficiency virus (FIV) DNA, respectively. Eighteen (94.7%) of the them were seropositive for toxoplasma. However, the only negative animal, a Leopardus pardalis, was the only FIV positve. These results suggest that the infection by FIV may have compromised its immune system and interfered with antibody production for toxoplasma.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Feline; Wild; Virus; Immunodeficiency; Toxoplasma gondii.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000500038

Bovine viral diarrhea diagnostic: immunohistochemistry standardization for routine
Provedor de dados: 45
Autores: Andrade,G.I.; Serra,C.V.; Barbosa-Stancioli,E.F.; Serakides,R.; Lobato,Z.I.P.
The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best results were seen using monoclonal antibodies and a commercial kit consisting of labelled streptavidin biotin (LSAB) reagents and the diaminobenzidine (DAB) substrate-chromogen reagent. The immunohistochemistry demonstrated to be an useful method for routine diagnosis for the controll and detection of BVDV infection.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Diarréia bovina a vírus; Imunoistoquímica; Bovine viral diarrhea; Virus; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500018

Possibility of vertical transmission of caprine arthritis-encephalitis virus in neonate kids
Provedor de dados: 45
Autores: Lara,M.C.C.S.H.; Birgel Junior,E.H.; Birgel,E.H.
Estudou-se a capacidade do vírus da artrite-encefalite dos caprinos (CAEV) infectar o feto ou o cabrito neonato pela via de transmissão transplacentária ou no momento do parto. Foram utilizados 26 cabritos recém-nascidos, filhos de cabras sororreagentes aos antígenos do CAEV e que nasceram de partos eutócicos. Na pesquisa de anticorpos séricos anti-CAEV, foi utilizada a técnica de imunodifusão em gel de ágar. Nenhum cabrito nasceu sororreagente aos antígenos do vírus, indicando que a possibilidade de transmissão vertical transplacentária da infecção foi menor do que 3,8% (&lt; 1:26).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprine; Caprine arthritis-encephalitis; Virus; CAEV; Lentivirus; Transmission.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400019

Histopathology of Anticarsia gemmatalis Hübner (Lepidoptera; Noctuidae) treated with Nucleopolyhedrovirus and Bacillus thuringiensis serovar kurstaki
Provedor de dados: 58
Autores: Knaak,Neiva; Fiuza,Lidia Mariana.
The Anticarsia gemmatalis is responsible for the use of chemical insecticides in the soybean culture, causing a significant increase in the costs of farming and a great unbalance in the ecosystem. The use of microbial agents, like Bacillus thuringiensis serovar kurstaki (Btk) and Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV), they are an alternative to chemical control of the pest insects. In the interaction analysis of the entomopathogenic bacteria and virus it is considered important the in vitro action mode of these microbiology control agents. Therefore, the present study aims the histopathological analysis of the A. gemmatalis larvae digestive system after the interaction in vivo of the entomopathogenic Btk and AgNPV, represented the Dipel and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virus; Bacteria; Biological control; Lepidoptera.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200017

Investigations of the Plum pox virus in Chile in the past 20 years
Provedor de dados: 117
Autores: Herrera,Guido.
Sharka disease, which is caused by Plum pox virus (PPV), is one of the most serious diseases affecting stone fruit trees around the world. Identified in Bulgaria in 1931, it was restricted to the European continent until 1992 when the virus was identified in Chile. It was subsequently verified in the USA, Canada, and Argentina. After 20 years since first detecting PPV in Chile, it seems clear that the disease cannot be eradicated in spite of various measures. Considering the seriousness of this problem for the domestic industry, a series of studies have been conducted to determine the distribution and degree of transmission of the disease, its biological and molecular characterization and epidemiological aspects, etc. The available information has allowed...
Tipo: Journal article
Palavras-chave: Sharka disease; Virus; Stone fruit.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000100009

Equine infectious anemia in the western region of Rio Grande do Sul, Brazil
Provedor de dados: 65
Autores: Barzoni,Cristiane Santin; Nogueira,Daniele Martinez Pereira; Marques,Gisele Dias; Diehl,Gustavo Nogueira; Pellegrini,Débora da Cruz Payão; Brum,Mário Celso Sperotto.
ABSTRACT: This study aimed to characterize the outbreaks of equine infectious anemia (EIA) identified, between the years 2009 and 2015, in the western region of the state of Rio Grande do Sul, Brazil. We identified 26 positive horses on 24 properties. Each positive property was considered an outbreak of the disease. The diagnoses were made using the agar gel immunodiffusion (AGID) test as a part of the sanitary checks conducted during animal transportation or certification of the horse´s sanitary status. The positive properties included farms or horse barns, and the infected animals were used for ranch work, sports, or reproduction. One outbreak was identified in animals that were being illegally transported from Argentina to Brazil. Fifteen outbreaks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transboundary disease; Illegal transport; EIA; Passive surveillance; Virus.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600451