Consulta: subjectFacets:"Virus identification"
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Data/hora: 09/06/2024 06:08:48

Production of policlonal antisera specific to plant viruses by rabbit oral immunization
Provedor de dados: 102
Autores: LIMA,J. ALBÉRSIO A.; LIMA,ROBERTO C.A.; GONÇALVES,M. FÁTIMA B.
Serological techniques are of great importance for plant virus identification and characterization. The major limiting factor for using these techniques for plant virus identification is the requirement of a good virus purified preparation to be used in immunizing animals for antiserum production. In the present study, two New Zealand rabbits were orally immunized with extracts from cowpea (Vigna unguiculata) plants systemically infected with Cowpea severe mosaic virus (CPSMV) and with extracts from papaya (Carica papaya) infected with Papaya lethal yellowing virus (PLYV). The leaf extracts were prepared in saline solution 0.15 M in the rate of 1:1 (w/v) and clarified by a centrifugation of 10,000 g for 10 min. The clarified extracts containing the viruses...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Serology; Immunology; Virus identification; CPSMV; PLYV.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000400016