Consulta: subjectFacets:"Yeast"
Registros recuperados: 75
Data/hora: 06/06/2024 16:22:54

Elaboración y caracterización de un aditivo biologicamente activo atraves de una fermentación en estado líquido.
Provedor de dados: 32
Autores: Hernández Cadena, Javier.
La melaza de caña de azúcar en el mercado internacional se comercializaba principalmente para la alimentación animal, actualmente tiene un mayor uso en la industria de la fermentación alcohólica y debido a su alto costo se ha venido limitado en la ganadería. Con el objetivo de establecer el nivel óptimo de melaza en un aditivo biológicamente activo (Vitafert), se estudió en diseño completamente al azar con arreglo factorial 3 niveles de melaza (5, 10 y 15%)  y 8 tiempos de fermentación líquida (0, 1, 2, 3, 6, 9, 15 y 30 día), con 3 repeticiones por tratamientos para las variables bromatológicas y fermentativas. Para las variables microbiológicas se utilizó un diseño completamente al azar con 3 tratamientos (niveles de melaza) y 8 repeticiones por...
Palavras-chave: Melaza; Yoghurt; Lactobacilos; Levaduras; Vitafert; Probióticos; Molasses; Yogurt; Lactobacillus; Yeast; Probiotics; Maestría; Producción Agroalimentaria en el Trópico.
Ano: 2010
URL: http://hdl.handle.net/10521/345

Evaluación de dos compuestos comerciales con cromo+selenio quelatados y Saccharomyces cerevisiae en dietas para ovinos en engorda.
Provedor de dados: 32
Autores: Reséndiz Hernández, Miriam.
El objetivo de este estudio fue evaluar el efecto de dos fuentes orgánicas queladas comerciales, Cr-levadura y Se-levadura, y de Saccharomyces cerevisiae (SC), en la digestibilidad in situ de la MS, FDN, FDA y PC; pH, AGV y N-NH3 en el rumen; consumo de MS, ganancia diaria de peso (GDP), conversión alimenticia (CA), peso y contenido de grasa de la canal de borregos. Los tratamientos fueron: 1) testigo (T; sin Cr, Se, ni SC); 2) T + Cr + Se; 3) T + SC; y 4) T + Cr + Se + SC, los cuales se asignaron al azar a 20 borregos criollos (29.88 ± 2.98 kg) en finalización para el experimento de variables productivas; y en un cuadro latino 4 x 4 con cuatro borregos machos (30 ± 2 kg) con cánula ruminal para evaluar degradación in situ y variables de fermentación...
Palavras-chave: Cromo; Selenio; Levadura; Ovinos; Digestibilidad; Rumen; Chromium; Selenium; Yeast; Sheep; Digestibility; Maestría; Ganadería.
Ano: 2012
URL: http://hdl.handle.net/10521/750

Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae
Provedor de dados: 69
Autores: Zhang,Min; Shi,Jun; Jiang,Li.
Background Yeast strains are exposed to numerous environmental stresses during industrial alcoholic fermentation. High temperature accumulated acetic acid, enhanced the growth inhibition and decreased ethanol production. Results In this study the influence of high temperature on the cellular and mitochondrial membrane integrity of Saccharomyces cerevisiae as well as reactive oxygen species (ROS) formation was investigated to understand the mechanisms of the high temperature fermentation process. However, increasing the temperature to 42°C resulted in a clear decrease in the cytoplasmic and mitochondrial membrane potential and an increase in intracellular ROS formation. It was also determined that the different thermostability between YZ1 and YF31 strains...
Tipo: Journal article
Palavras-chave: Bioethanol; Random amplified polymorphic DNA (RAPD); Yeast.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300010

Yeast lipases: enzyme purification, biochemical properties and gene cloning
Provedor de dados: 69
Autores: Vakhlu,Jyoti.
Lipases are placed only after proteases and carbohydrases in world enzyme market and share about 5% of enzyme market. They occur in plants, animals and microorganisms and are accordingly classified as plant, animal and microbial lipases. Wherever they exist, they function to catalyze hydrolysis of triglycerides to glycerol and fatty acid. Like carbohydrases and proteases, lipases of microbial origin enjoy greater industrial importance as they are more stable (compared to plant and animal lipases) and can be obtained in bulk at low cost. Majority of yeast lipases are extracelluar, monomericglycoproteins with molecular weight ranging between ~33 to ~65 kD. More than 50% reported lipases producing yeast, produce it in the forms of various isozymes. These...
Tipo: Journal article
Palavras-chave: Candida; Geotrichum; Lipase; Trichosporon; Yeast.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100010

Lead biosorption onto waste beer yeast by-product: a means to decontaminate effluent generated from battery manufacturing industry
Provedor de dados: 69
Autores: Parvathi,Krishnakumar; Nagendran,Ramachandramurthy; Nareshkumar,Radhakrishnan.
This paper projects the potential of waste beer yeast Saccharomyces cerevisiae in biosorbing lead from battery manufacturing industrial effluent. Experiments were carried out as a function of pH, biosorbent concentration, lead concentration and agitation speed. Specific lead uptake of 2.34 mg/g was recorded and the data gave good fits for Freundlich and Langmuir models with Kf and Qmax values of 0.5149 and 55.71 mg/g. The roles played by amines, carboxylic acids, phosphates, sulfhydryl group and lipids in lead biosorption were studied. Electrostatic attraction may be the mechanism of biosorption. The extent of contribution of the functional groups and lipids to lead biosorption was in the order: carboxylic acids &gt; lipids &gt; amines &gt; phosphates....
Tipo: Journal article
Palavras-chave: Biosorption; Electrostatic attraction; Freundlich; Langmuir; Functional group; Lead; Yeast.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100009

From yeast to humans: Understanding the biology of DNA Damage Response (DDR) kinases
Provedor de dados: 74
Autores: Cussiol,José Renato Rosa; Soares,Bárbara Luísa; Oliveira,Francisco Meirelles Bastos de.
Abstract The DNA Damage Response (DDR) is a complex network of biological processes that protect cells from accumulating aberrant DNA structures, thereby maintaining genomic stability and, as a consequence, preventing the development of cancer and other diseases. The DDR pathway is coordinated by a signaling cascade mediated by the PI3K-like kinases (PIKK) ATM and ATR and by their downstream kinases CHK2 and CHK1, respectively. Together, these kinases regulate several aspects of the cellular program in response to genomic stress. Much of our understanding of these kinases came from studies performed in the 1990s using yeast as a model organism. The purpose of this review is to present a historical perspective on the discovery of the DDR kinases in yeast...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Genome instability; DNA damage response; Cell cycle checkpoint; Kinase.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200302

Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain
Provedor de dados: 74
Autores: Morais Junior,Marcos Antonio de; Santos,José Ferreira dos; Henriques,João Antonio Pegas.
The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Genetic recombination; RecA gene; Sporulation; Yeast.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200016

Trial of probiotics to prevent the vertebral column compression syndrome in rainbow trout (Oncorhynchus mykiss Walbaum)
Provedor de dados: 5
Autores: Aubin, Joel; Gatesoupe, Joel; Labbe, Laurent; Lebrun, Luc.
Two probiotics were tested as alternative treatment to limit the prevalence of the vertebral column compression syndrome (VCCS) in rainbow trout, compared with a preventive treatment with florfenicol. Either the antibiotic, or a lactic acid bacterium, Pediococcus acidilactici, or a yeast, Saccharomyces cerevisiae var. boulardii, was introduced into experimental diets, which were compared with the control diet without supplementation. The antibiotic caused some mortality during the first week after treatment, likely because of the toxicity of the compound. However, this antibiotic treatment limited the occurrence of VCCS to 3%, vs. 13% of fish affected in the control group. Pediococcus acidilactici provided the same level of protection as the antibiotic,...
Tipo: Text
Palavras-chave: Carnobacterium; Debaryomyces hansenii; Pseudomonas; Antibiotic; Lactic acid bacterium; Yeast; Spinal deformities.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-752.pdf

Live yeasts in the gut: Natural occurrence, dietary introduction, and their effects on fish health and development
Provedor de dados: 5
Autores: Gatesoupe, Joel.
This minireview summarizes the present state of knowledge concerning the importance of yeasts in fish gut. Yeasts have been commonly isolated in the gastrointestinal tract, and high population densities were sometimes noted in healthy fish, but the data were quite variable in terms of colony counts and taxonomical diversity. Rhodotorula sp. seemed relatively frequent in both marine and freshwater fish, and Debaryomyces hansenii has been found to be dominant in rainbow trout. Some other dominant strains have been described, such as Metschnikowia zobelii, Trichosporon cutaneum, and Candida tropicalis in marine fish, and Candida sp., Saccharomyces cerevisiae, and Leucosporidium sp. in rainbow trout. The natural proliferation of yeasts in fish mucus may be...
Tipo: Text
Palavras-chave: Immune system; Growth promotion; Gut maturation; Gastrointestinal microbiota; Probiotics; Yeast.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2144.pdf

Cross effects of the strain of dietary Saccharomyces cerevisiae and rearing conditions on the onset of intestinal microbiota and digestive enzymes in rainbow trout, Onchorhynchus mykiss, fry
Provedor de dados: 5
Autores: Wache, Yann; Auffray, Francoise; Gatesoupe, Joel; Zambonino, Jose-luis; Gayet, Vincent; Labbe, Laurent; Quentel, C.
Two strains of Saccharomyces cerevisiae were tested as probiotics for rainbow trout fry, during the first month of feeding. Each strain was introduced into separate diets, at the rate of 106 CFU g&amp;#8722; 1 and their effects were compared with those of a control diet. Two rearing conditions were simultaneously compared, to test the adaptability of the probiotic treatment. From start feeding onwards, the water supply came from either spring or river, resulting in two different temperature ranges, 11¿11.5 and 7¿8 °C respectively. Growth and development were optimal in spring water, while some delay was observed with colder river water. A slight but significant increase in mortality was also observed in the river group. In all groups, the counts of...
Tipo: Text
Palavras-chave: Debaryomyces hansenii; Yeast; Brush border membrane; Gut maturation; Larval development; Start feeding.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1816.pdf

Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds
Provedor de dados: 86
Autores: Fialho,Maurício Batista; Moraes,Maria Heloisa Duarte de; Tremocoldi,Annelise Roberta; Pascholati,Sérgio Florentino.
The objective of this work was to evaluate the potential of an artificial mixture of volatile organic compounds (VOCs), produced by Saccharomyces cerevisiae, to control Sclerotinia sclerotiorum in vitro and in bean seeds. The phytopathogenic fungus was exposed, in polystyrene plates, to an artificial atmosphere containing a mixture of six VOCs formed by alcohols (ethanol, 3-methyl-1-butanol, 2-methyl-1-butanol and phenylethyl alcohol) and esters (ethyl acetate and ethyl octanoate), in the proportions found in the atmosphere naturally produced by yeast. Bean seeds artificially contamined with the pathogen were fumigated with the mixture of VOCs in sealed glass flasks for four and seven days. In the in vitro assays, the compounds 2-methyl-1-butanol and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Alternative control; Fumigation; White mold; Yeast.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000200004

Basil conservation affected by cropping season, harvest time and storage period
Provedor de dados: 86
Autores: Silva,Franceli da; Santos,Ricardo Henrique Silva; Andrade,Nélio José de; Barbosa,Luiz Cláudio Almeida; Casali,Vicente Wagner Dias; Lima,Renato Ribeiro de; Passarinho,Ricardo Vaz de Melo.
Fresh basil (Ocimum basilicum L.) is used in food, phytotherapic industry, and in traditional therapeutic, due to its essential oil content and composition. Nevertheless basil can not be kept for long periods after harvest and its quality can be reduced. This work aimed to assess the influence of the season and harvest time in the postharvest conservation of basil stored for different periods. Basil was harvested at 8 am and 4 pm both in August/1999 and January/2000. Cuttings were conditioned in PVC packages and stored for 3, 6, and 9 days. During storage, chlorophyll content, essential oil content and composition were determined as well as microbiological analyses were carried out. Harvest season and the days of storage influenced the final content of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocimum basilicum; Eugenol; Linalool; Chlorophyll; Mold; Yeast.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000400002

Use of prebiotics and probiotics of bacterial and yeast origin for free-range broiler chickens
Provedor de dados: 89
Autores: Pelícia,K; Mendes,AA; Saldanha,ESPB; Pizzolante,CC; Takahashi,SE; Moreira,J; Garcia,RG; Quinteiro,RR; Paz,ICLA; Komiyama,CM.
This study aimed to evaluate the effects of probiotics and prebiotics of bacterial and yeast origin on the performance, development of the digestive system, carcass yield and meat quality of free-range broiler chickens. Five hundred and sixty male chicks of the strain ISA S757-N were reared from one to 84 days old. The birds were distributed in four treatments according to a completely randomized block design: T1 = Control, T2 = Probiotics and Prebiotics of bacterial origin, T3 = Probiotics and prebiotics of yeast origin, T4 = Probiotics and prebiotics of bacterial origin + probiotics and prebiotics of yeast origin. There were four repetitions with 35 birds per repetition, and the birds had access to a pasture area after 35 days of age. Characteristics...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Free-range broiler chickens; Digestive system; Probiotics; Symbiotics; Yeast.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000300006

Aerobic fungi in the rumen fluid from dairy cattle fed different sources of forage
Provedor de dados: 96
Autores: Almeida,Patrícia Natalicia Mendes de; Duarte,Eduardo Robson; Abrão,Flávia Oliveira; Freitas,Cláudio Eduardo Silva; Geraseev,Luciana Castro; Rosa,Carlos Augusto.
The objective of this study was to evaluate the aerobic microbiota of the rumen fluid from Holstein cows and heifers fed different tropical forage in the north of Minas Gerais, Brazil. A total of 30 samples of rumen fluid from cows fed with sorghum silage were collected: 32 from cows fed Brachiaria brizantha, 12 from heifers that received sorghum silage and 11 from calves fed sugar cane foliage. The culture was carried out using the agar Sabouraud medium and the solid C medium, containing microcrystalline cellulose. The isolated mycelial fungi were identified by microculture technique and yeasts by micromorphological and physical-chemical analysis. Specific identification for yeasts was confirmed by ribosomal DNA sequence analysis. The presence of fungal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy farming; Forage supplementation; Rumen microbiota; Semiarid; Tropical pasture; Yeast.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001100006

Yeast fermented additive enhances broiler growth
Provedor de dados: 96
Autores: Yasar,Sulhattin; Yegen,Mustafa Kemal.
ABSTRACT We investigated the effects of dietary supplementations of yeast (YFA) or non-yeast fermented additives (NYFA) at 5.0 and 10 g kg−1 on the performance parameters and the size of digestive organs of broiler chickens. An isocaloric and isonitrogenous basal diet used as negative control was added with 5.0 or 10.0 g kg−1 of YFA or NYFA. Each of five experimental diets was given to broiler chickens kept in three randomized floor pens, each with 21 birds, from one-day old to 42 days old. Although the feed intake of broiler chickens was not significantly influenced by the diets, there were significant increases in weight gain and improvements in feed conversion ratio and carcass yield in broiler chickens fed the diets supplemented with 5.0 and 10 g kg−1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler; Fermentation; Microbial additive; Yeast.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017001000814

Animal performance and nutrient digestibility of feedlot steers fed a diet supplemented with a mixture of direct-fed microbials and digestive enzymes
Provedor de dados: 96
Autores: Encinas,Christian Michael Alvarez; Villalobos,Guillermo Villalobos; Viveros,Joel Domínguez; Flores,Gabriela Corral; Almora,Enrique Alvarez; Rangel,Francisco Castillo.
ABSTRACT The objective of this study was to evaluate the effect of a mixture of direct-fed microbials (DFM), yeast, and digestive enzymes on animal performance in feedlot cattle, as well as digestibility when finishing feedlot cattle. Thirty crossbreed (Charolais × Beefmaster) steers averaging 15 months old and 321.83±3.73 kg of initial body weight were used. Animals were randomly assigned to one of two treatment groups: control (basal diet) and a mixture of DFM, yeast, and digestive enzymes (basal diet + 30 g of the mixture). Each group contained fifteen experimental units. Animals were fed individually twice a day, and dry matter intake (DMI) was recorded daily. Body weights were recorded initially and subsequently at 28-day intervals for a total of 140...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Finishing; Probiotics; Saccharomyces cerevisiae; Yeast.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100615

Silage microbiology and its control through additives
Provedor de dados: 96
Autores: Muck,Richard E.
Ensiling is a method of preserving a moist crop. A moist crop can support the growth of a wide range of microorganisms, most of which will degrade the nutrient value to livestock. However, ensiling generally controls microbial activity by a combination of an anaerobic environment and a natural fermentation of sugars by lactic acid bacteria on the crop. This fermentation and the resulting low pH primarily suppress the growth of other anaerobic microorganisms. The fermentation can also inhibit yeasts, molds and aerobic bacteria, but the anaerobic environment is essential to preventing most of the spoilage microorganisms from growing. Inoculants have become the dominant additives for making silage. Homofermentative strains help guarantee a rapid suppression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridia; Enterobacteria; Inoculant; Lactic acid bacteria; Mold; Yeast.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300021

Changes in trehalose content of baker's yeast as affected by octanoic acid
Provedor de dados: 63
Autores: Gutierrez,L.E.
Octanoic acid inhibited ethanolic fermentation by Saccharomyces cerevisiae (bakers yeast) and the trehalose accumulation, however did not affect the endogenous degradation of trehalose. This inhibition may be explained by the binding of octanoic acid to hexokinase or other proteins of plasma membrane because they are not necessary for endogenous fermentation. The degradation of trehalose may be due to an activation of trehalase.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trehalose; Yeast; Saccharomyces; Octanoic acid.
Ano: 1993
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161993000300020

Fermentation of irradiated sugarcane must
Provedor de dados: 63
Autores: Alcarde,André Ricardo; Walder,Júlio Marcos Melges; Horii,Jorge.
Bacillus and Lactobacillus are bacteria that usually contaminate the ethanolic fermentation by yeasts and may influence yeast viability. As microorganisms can be killed by ionizing radiation, the efficacy of gamma radiation in reducing the population of certain contaminating bacteria from sugarcane must was examined and, as a consequence, the beneficial effect of lethal doses of radiation on some parameters of yeast-based ethanolic fermentation was verified. Must from sugarcane juice was inoculated with bacteria of the genera Bacillus and Lactobacillus. The contaminated must was irradiated with 2.0, 4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation. After ethanolic fermentation by the yeast (Saccharomyces cerevisiae) the total and volatile acidity produced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Gamma radiation; Yeast.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400011

การศึกษาการเพิ่มระดับโปรตีนของกากมะพร้าวสดและแห้ง โดยกระบวนการหมักยีสต์และยูเรีย
Provedor de dados: 106
Autores: Narumon Somkuna; Jarous Sawangtap; Jiraprapa Rodjarkken; Surasak Uttarawichian.
The objective of this study was to compare the increasing protein level of fresh and dry coconut meal by urea-yeast (Saccharomyces cerevisiae) fermentation process. The design was 2 X 8 factorial in CRD : factor A composed of 2 factors (dry and fresh coconut meal) and factor B composed of 8 factors (day of fermentation: 5, 10, 15, 20, 25, 30, 35 and 40 days). The samples of each day of fermentation were collected for crude protein analysis. The data was analyzed by using Analysis of Variance (ANOVA). The results were shown that the protein level at the different days of fermentation was different in fresh and dry coconut meal (P&lt;0.05) which protein level of fresh coconut meal at day 10 was highest (34.40 %). While protein level in dry coconut meal was...
Tipo: PhysicalObject
Palavras-chave: Fresh coconut meal; Dry coconut meal; Coconut copra; Kernel meal; Fermentation process; Yeast; Saccharomyces cerevisiae; Urea; Animal feed; Crude protein level; กากมะพร้าวสด; กากมะพร้าวแห้ง; มะพร้าว; กระบวนการหมัก; ยีสต์; ยูเรีย; ระยะเวลาหมัก; อาหารสัตว์; โปรตีนหยาบ; การเพิ่มโปรตีน; ระดับโปรตีน.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5742

Nitrogen source and pH interact and modulate lipase secretion in a non-clinical strain of Candida parapsilosis
Provedor de dados: 3
Autores: Ribas, Rodolfo Krüger da Câmara; Carboni, Diórgenes dos Santos; Cazarolli, Juciana Clarice; Flôres, Simone Hickmann; Ramirez-Castrillon, Maurício; Valente, Patricia.
Lipases (E.C. 3.1.1.3) are serine-hydrolases, and act on long chain fatty acid ester bonds. They exhibit specific and enantioselective activities, which are desirable for many industrial applications. This study aimed at screening and optimizing the production of lipases by wild yeast strains from a variety of substrates, as well as characterizing the enzyme. An initial selection was made in oxygenated oil-supplemented minimum medium, and the enzymatic activity of the supernatant was tested over p- nitrophenyl palmitate. One-hundred and twenty-four yeast strains from different substrates were tested, and twenty-three showed significantly higher lipolytic activity (p &amp;lt;0.01). One yeast in particular, QU110, showed best lipase production and therefore...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-NPP; Tryptone; Yeast; Palmitate; Response Surface Model; Serine-hydrolase.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/45481

Synthesis and evaluation of arylamidine derivatives for new antimicrobial and cytotoxic activities
Provedor de dados: 42
Autores: MONTE,ZENAIDE S.; SILVA,AMANDA M.; LIMA,GLÁUCIA M.S.; SILVA,TERESINHA G. DA; MARQUES,KARLA M.R.; RODRIGUES,MARIA D.; FALCÃO,EMERSON P.S.; MELO,SEBASTIÃO J.
ABSTRACT A series of arylamidines 3a-j was designed, synthesized and investigated for antimicrobial activity. Structures of the compounds were confirmed by IR, 1H-NMR and 13C-NMR and a 2D spectroscopic study was performed. A preliminary screening of the antimicrobial tests clearly showed that three out of ten arylamidines, viz, 3f, 3g and 3i, were effective against all the gram-negative bacteria: Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella enteric; and against the yeast, candida albicans. Further, the Minimum Inhibitory Concentrations (MIC) against the bacteria and yeast were determined. All compounds 3a-d, 3f, 3g, 3i and 3j were also investigated for their low cytotoxic effects on tested cell lines. Compounds 3d and 3f were the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arylamidines; 2D spectroscopy; Gram-negative bacteria; Yeast; Cytotoxicity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301051

Antifungal susceptibility of yeasts isolated from anthropogenic watershed
Provedor de dados: 42
Autores: MILANEZI,ANA C.M.; WITUSK,JOÃO P.D.; VAN DER SAND,SUELI T.
Abstract Yeasts are unicellular fungi widespread in the environment, and studies suggest that there is a positive correlation between yeast and polluted aquatic environments. The aim of this study was to analyze the diversity and resistance of yeasts isolated from water samples collected along the Arroio Dilúvio in Porto Alegre to antifungals. Yeast isolates from the Arroio Dilúvio were subjected to susceptibility assays against antifungals using the minimum inhibitory concentration (MIC) test, and amplification of the ITS1-5.8S-IT2 region; in addition, the ITS-5.8S region was sequenced for some of the isolates. The amplification product was subjected to PCR-RFLP, and the restriction profile allowed the construction of a dendrogram. Susceptibility tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Antifungal; Watershed; Resistance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100601

Athelia (Sclerotium) rolfsii in Allium sativum: potential biocontrol agents and their effects on plant metabolites
Provedor de dados: 42
Autores: CAVALCANTI,VYTÓRIA P.; ARAÚJO,NEILTON A.F.; SCHWANESTRADA,KÁTIA R.F.; PASQUAL,MOACIR; DÓRIA,JOYCE.
Abstract Garlic (Allium sativum L.) plays an important role in popular culture due to its dietary and medicinal uses. It is also used to produce a wide range of pharmacologically interesting molecules. Several pathogens affect garlic plants, especially Athelia (Sclerotium) rolfsii, a fungus that is widespread and causes large economic losses. It causes direct damage to crops and leads to plant stress, which induces secondary metabolite production in plants. The use of microorganisms as biocontrol agents may induce the production of beneficial metabolites in plants that will protect it and promote resistance to pathogen attack. In addition to suppressing disease, biological control agents may have elicitor effects that could induce an increase in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Phytopathogen; Yeast; Bacillus spp.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703949

Candida albicans Clinical Isolates from a Southwest Brazilian Tertiary Hospital Exhibit MFS-mediated Azole Resistance Profile
Provedor de dados: 42
Autores: PINTO,ANA CAROLINA C.; ROCHA,DEBORA A.S.; MORAES,DANIEL C. DE; JUNQUEIRA,MARIA L.; FERREIRA-PEREIRA,ANTONIO.
Abstract: ﻿Candida albicans is the most frequent fungal species that causes infections in humans. Fluconazole is the main antifungal used to treat Candida infections, and its prolonged and indiscriminate use for the last decades are the most established causes which originated resistant strains. Fungal drug resistance is associated to alterations in ERG11 gene and overexpression of multidrug resistance (MDR) transporters belonging to two families: ATP-binding cassette (ABC) and Major Facilitator Superfamily (MFS). To evaluate the role of MFS transporters in azoles resistance of C. albicans clinical strains, this study aimed to analyze four Candida albicans clinical isolates from the University Hospital in Juiz de Fora (Minas Gerais/Brazil), selected in our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida; Fluconazole; MFS; Multidrug resistance; Yeast.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500603

Probiotic, antibiotic and combinations in Nile tilapia juveniles culture
Provedor de dados: 42
Autores: FERREIRA,ANDRE L.; AMORIM,MATHEUS P.S.; SOUZA,EMILENE R.; SCHORER,MARIANNE; CASTRO,GUSTAVO H.F.; PEDREIRA,MARCELO M.
Abstract: This study aimed to evaluate the effect of the commercial probiotic (Saccharomyces cerevisiae), antibiotic (florfenicol), and its combination for Nile tilapia culture. The experiment was arranged in a completely randomized design with five replications, and five treatments diets: Control: water and diet without additives; YD: yeast in the diet (1 g Kg-1); AW: antibiotic in the water (0.002 g L-1); AWYD: antibiotic in the water, and yeast in the diet (0.002 g L-1 and 1 g Kg-1); and AD: antibiotic in the diet (0.01 g kg-1). The growth parameters as total and standard length, weight, weight gain, biomass, Fulton’s condition factor, specific growth rate and plasma cortisol of tilapia did not show the difference between the treatments. The survival...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal welfare; Florfenicol; Yeast; Prebiotic and probiotic; Growth promoter.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700856

Leveduras associadas a Spermologus rufus Boheman (Coleoptera: Curculionidae)
Provedor de dados: 44
Autores: Barreto,Marliton R.; Barreto,Eriana S.; Anjos,Norivaldo.
Larvae and adults of Spermologus rufus Boheman can cause severe damage to seeds of Brazilian pine, Araucaria angustifolia. In this study, yeasts were isolated from seeds of A. angustifolia and from the digestive tract of S. rufus to verify their similarity and determine the existence of feeding sites in order to elaborate an artificial diet for this insect. Pichia spp. were found in seeds and Pichia spp. and P. stipitis Pignal in the digestive tract of S. rufus. The low variability among observed microorganisms suggests a food preference, which can facilitate the elaboration of artificial diets.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Coleoptera; Curculionidae; Yeast; Pichia; Artificial diets; Brazilian pine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000200017

Identification of Candida species isolated from cows suffering mastitis in four Brazilian states
Provedor de dados: 45
Autores: Sartori,L.C.A.; Santos,R.C.; Marin,J.M.
A mastite representa um dos problemas mais sérios que afetam as fazendas de gado leiteiro. As mastites clínica e subclínica em bovinos, devido a leveduras, são causadas principalmente por espécies do gênero Candida. O objetivo deste trabalho foi identificar quais espécies dessa levedura estavam presentes em amostras de leite obtidas da glândula mamária de vacas apresentando mastite. Um total de 428 amostras de leite foi coletado nos estados de São Paulo, Paraná, Santa Catarina e Rio Grande do Sul. A identificação foi realizada por meio de critérios fenotípicos e fisiológicos. Foi possível isolar seis espécies de Candida em 55 (12,8%) das amostras. As espécies mais frequentes foram C. krusei(34,6%), C. parapsilosis(25,4%), C. tropicalis (18,2%) e C....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cow; Bovine mastitis; Milk; Yeast; Candida.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501615

The influence of probiotic additives or multienzyme composition on blood biochemical parameters and milk quality of Lithuanian Black-and-White cattle
Provedor de dados: 45
Autores: Dailidavičienė,J.; Budreckienė,R.; Gružauskas,R.; Kerzienė,S.; Andrulevičiūtė,V.; Sinkevičienė,I.
ABSTRACT The aim of the study was to determine the effect of Saccharomyces cerevisiae, multienzyme composition supplementation on milk yield, quality, blood biochemical parameters of Lithuanian-Black-and-White cows. For this reason 28 cows were divided into four groups (three experimental and one control) each with seven cows fed balanced ration (control group) and following experimental groups: addition of 40g supplement of live yeast with organic selenium (group A); 40g supplement of live yeast with aromatic additives (group B); and 0.2g supplement of multienzyme composition (group C) during a 90 days period. The study showed that milk yield was 2.64%, 1.75%, 1.4% higher in groups A, B, C respectively, comparing with the control group. The milk SCC in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cow; Probiotic; Yeast; Milk yield; Blood.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300939

Saccharomyces uvarum mannoproteins stimulate a humoral immune response in mice
Provedor de dados: 52
Autores: Darpossolo,Fernanda Patrícia Brito; Eto,Silas Fernandes; Venancio,Emerson José; Castro-Goméz,Raul Jorge Hernan.
Yeasts discarded in industrial processes can be used as a nutritional supplement and to extract cellular components with biotechnological aims. In this study, the humoral immune response of Swiss mice treated with mannoproteins (MP) from the yeast Saccharomyces uvarum was evaluated. The mice were treated with MPs at different doses and times and inoculated with 2% sheep red blood cells. An increase in total Ig in mice treated with 100 μg of MP at the time of immunization or 24 h before was observed in the primary immune response; in the secondary immune response, an increase was observed in total Ig values for all groups, and an increase of IgG was observed in the mice treated with MPs (100 μg) at the time of immunization or 24 h before. These results show...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycoprotein; Swiss mice; Antibody production; Yeast; Cell wall.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400016

Comparison of two lipid extraction methods produced by yeast in cheese whey
Provedor de dados: 52
Autores: Castanha,Rodrigo Fernandes; Morais,Lilia Aparecida Salgado de; Mariano,Adriano Pinto; Monteiro,Regina Teresa Rosim.
This work aimed to evaluate nine strains of yeast, previously identified as good producers of lipids in honey medium, for selecting the most suitable strain for the production of lipids in cheese whey medium and compared two well known extraction methods of lipids from the culture medium. The highest yield of total lipids was 1.27 g.L-1 produced by Cryptococcus laurentii 11. A comparison was made between the two culture media: cheese whey and liquid YEPG, and two lipid extraction methods: Bligh and Dyer and Folch et al. for C. laurentii. The experiments were performed with 2² full factorial design using two factors and two levels. Lipid content was higher in cheese whey and there was no difference in the extraction methods statistically. The method of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cheese whey; Yeast; Lipid extraction; Microbial oil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400013

Determination of the Effect of the Elimination Diet Applied for Overweight and Obese People with Food Intolerance on Body Composition and Biochemical Parameters
Provedor de dados: 52
Autores: Gubur,Sema.
ABSTRACT Food Intolerance, is a reaction against food, but not immunological manner, and may be confused with real food allergies. In this study, effects of special weight-loss diet together with an elimination diet on body composition and biochemical parameters of overweight and obese people who were diagnosed with food intolerance were investigated. The study group consists of 20 patients in total who were followed-up and treated in Yorktest Turkey Laboratory; who were diagnosed with food intolerance, and whose BMI was &gt; 26kg/m2. Bloodletting for these patients was executed with Lancet from their fingertips, and the blood drawn from these patients was assessed via enzyme-linked immunosorbent assay (ELİSA) method, and food reactions of patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food intolerance; Obesity; IgG; Elimination diet; Yeast.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100314

Production of alcohol by simultaneous saccharification and fermentation of low-grade wheat flour
Provedor de dados: 52
Autores: Neves,Marcos Antonio das; Kimura,Toshinori; Shimizu,Naoto; Shiiba,Kiwamu.
Two samples of low-grade wheat flour, namely low-grade 1 (LG1) and low-grade 2 (LG2), with different carbohydrate and fibrous content, were used as substrates. The samples were liquefied using various concentrations of alpha- or beta-amylase, in order to optimize the production of fermentable sugars; the enzyme alpha-amylase revealed higher performance. After liquefaction, the simultaneous saccharification and fermentation was conducted in a jar fermentor. Amyloglucosidase was used for saccharification, and dry baker's yeast, S. cerevisiae, for fermentation simultaneously. Glucose was consumed promptly in both cases, LG1 and LG2; ethanol production was considerably higher in LG1 (38.6 g/L), compared to LG2 (24.9 g/L). The maximum ATP production was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low grade; Wheat flour; Yeast; Fermentation; Ethanol.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400017

Optimization of biomass production with copper bioaccumulation by yeasts in submerged fermentation
Provedor de dados: 52
Autores: Arakaki,Andréa Haruko; Souza Vandenberghe,Luciana Porto de; Soccol,Vanete Thomaz; Masaki,Ryu; Rosa Filho,Ernani Francisco da; Gregório,Alexsandro; Soccol,Carlos Ricardo.
The objective of this work was to study the production of biomass with copper bioaccumulation in submerged fermentation using sugarcane molasses. Candida pelliculosa BARU 05 isolated from Baru (Dipteryx alata) was selected for its good capacity to accumulate the copper. Fermentation was carried out using the medium composed by sugarcane molasses at 5 °Brix enriched with (g/L) CuSO4.5H2O 0.1; yeast extract, 10.0; (NH4)2SO4, 5.0 ; KH2PO4, 5.0 MgSO4, 0.5, inoculum 10 % of total volume (100 ml), pH 6.0, and incubation at 30 °C, 120 rpm for 120 h. After three steps of optimization an uptake of 95.04% and 13.397 g/L biomass were obtained. The kinetics of copper bioaccumulation and biomass production was followed in a 10- liter bioreactor in a batch and fed-batch...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioaccumulation; Baru; Yeast; Copper; Sugarcane molasses.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500021

Identification of yeasts Isolated from processed and frozen cocoa (Theobroma cacao) pulp for wine production
Provedor de dados: 52
Autores: Trindade,Rita de Cássia; Resende,Maria Aparecida de; Barreto,Eriana Gomes Serpa; Mendes,Taniella de Carvalho; Rosa,Carlos Augusto.
The alternative use of cocoa (Theobroma cacao) for wine production was tested. The pulp samples, obtained from Formosa farm, Itacaré, Brazil, were diluted, homogenized and inoculated on Sabouraud dextrose agar medium (SDA) and incubated at 28º C for 5-8 days. Selected colonies were tested for the ability to ferment cocoa pulp and divided into fermentative, non-fermentative and weak/late fermentative species. Isolates characterized as fermentative were further tested in a small-scale wine production plant and identified. Species from the genus Brettanomyces constituted the main fermentative yeasts, with the exception of two Kloeckera apis samples. The final wine product was normally pale or clear, making clarification unnecessary, and with a sweet or dry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Cocoa pulp; Fermentation; Wine production; Brettanomyces sp.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300013

Production and Optimization of Killer Toxin in Debaryomyces hansenii Strains
Provedor de dados: 52
Autores: Çorbacı,Cengiz; Uçar,Füsun Bahriye.
ABSTRACT Postharvest diseases of fruits and vegetables result in critical losses of production in worldwide. The losses often are caused by fungi and nowadays, most fungal pathogens are controlled by several strategies such as the use of fungicides. However, most of the fungicides are chemical-based compounds and are dangerous to human health and the nature. Therefore, the discovery of healthy and reliable strategies is crucial to control of fungal pathogens. In the paper, it was aimed to evaluate and characterize yeast isolates previously isolated from dairy products for the production of killer toxin. A total of 18 yeasts have been found to produce antagonistic behavior against susceptible fungal species. All of the yeasts expressing killer character...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Killer toxin; Optimization; Postharvest disease; Yeast.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100402

Apple wine processing with different nitrogen contents
Provedor de dados: 52
Autores: Alberti,Aline; Vieira,Renato Giovanetti; Drilleau,Jean Françoise; Wosiacki,Gilvan; Nogueira,Alessandro.
The aim of this work was to evaluate the nitrogen content in different varieties of apple musts and to study the effect of different nitrogen concentrations in apple wine fermentation. The average total nitrogen content in 51 different apples juices was 155.81 mg/L, with 86.28 % of the values above 100 mg/L. The apple must with 59.0, 122.0 and 163.0 mg/L of total nitrogen content showed the maximum population of 2.05x 10(7); 4.42 x 10(7) and 8.66 x 10(7) cell/mL, respectively. Therefore, the maximum fermentation rates were dependent on the initial nitrogen level, corresponding to 1.4, 5.1 and 9.2 g/L.day, respectively. The nitrogen content in the apple musts was an important factor of growth and fermentation velocity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple juice fermentated; Fermentation rate; Cider; Yeast.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300017

Selection of Xilose-Fermenting Yeast Strains
Provedor de dados: 52
Autores: Silva,Rosimeire Oenning da; Cereda,Marney Pascoli; Gomes,Eleni; Martins,Gisele Marta; Pagnocca,Fernando Carlos; Silva,Roberto da.
ABSTRACT In Brazil, ethanol is obtained by fermentat of sugar cane juice using Saccharomyces cerevisiae. The cane juice extraction generates the bagasse that has been used for obtaining generation biofuel. However, the sugarcane bagasse has 30% pentose that cannot be fermented to ethanol by S. cerevisiae. Thus the aim of this study was to isolate a yeast able to ferment xylose to ethanol. Samples of cane juice and flowers were used for the isolation of 165 strains that were then screened for ethanol production using plate testing. Among them, the ethanol positive strains Wickerhamomyces anomalus, Schizosaccharomyces pombe and Starmerella meliponinorum were selected for a xylose fermentation assay, using a semi-synthetic and bagasse hydrolysate as must. S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylose; Ethanol; Yeast; Sugarcane bagasse.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100419

Growth of Nile tilapia Oreochromis niloticus fed diets with different levels of proteins of yeast
Provedor de dados: 52
Autores: Medri,Vandir; Medri,Waldir; Caetano Filho,Mauro.
This experiment was based on observations of 72 juveniles of Nile tilapia (Oreochromis niloticus), sexually reverted with an initial mean weight of 37.27 ± 4.92g, distributed in 12 cages of 100 l to evaluate the effects of the yeast inclusion as proteins source in the diet. The fishes were distributed in a completely randomized design with four treatments (0; 20; 40; and 60%) of yeast protein in substitution to the protein of traditional sources with three repetitions. Effects of the treatments were not observed (p &gt; 0.05) on the survival and to food conversion. It was observed a quadratic effect on weight gain (Y = 73.39 + 0.173X - 0.0034X²; R²= 0.9986). It was concluded the best level of yeast inclusion as source proteins in the diet for reversed Nile...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Cages; Tilapia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300024

Determination of the Effect of the Elimination Diet Applied for Overweight and Obese People with Food Intolerance on Body Composition and Biochemical Parameters
Provedor de dados: 52
Autores: Gubur,Sema.
ABSTRACT   Food Intolerance, is a reaction against food, but not immunological manner, and may be confused with real food allergies. In this study, effects of special weight-loss diet together with an elimination diet on body composition and biochemical parameters of overweight and obese people who were diagnosed with food intolerance were investigated. The study group consists of 20 patients in total who were followed-up and treated in Yorktest Turkey Laboratory; who were diagnosed with food intolerance, and whose BMI was &gt; 26kg/m2. Bloodletting for these patients was executed with Lancet from their fingertips, and the blood drawn from these patients was assessed via enzyme-linked immunosorbent assay (ELİSA) method, and food reactions of patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food intolerance; Obesity; IgG; Elimination diet; Yeast.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100305

Application of the taguchi’s loss function in the breeding of nile tilapia fed with different levels of alcohol yeast
Provedor de dados: 52
Autores: Medri,Vandir; Pereira,Geni Varéa; Leonhardt,Júlio Hermann.
Studies were conducted to evaluate the effect of 10%, 20% and 30% ration substitutions for distillery yeast on the function Taguchi’s loss using two hundred and forty 45-day-old fingerlings of Nile tilapia (Oreochromis niloticus), sexually reverted with an initial average weight of 1.25 ± 0.14 g placed in amianthus box.. The average results obtained for the limnological parameters of the water quality control through chemical analysis were considered normal. Taguchi’s losses for the total production, for the dead and discarded fishes did not show correlation for the different levels of alcohol yeast inclusion, showing that the choice of the yeast level in the ration for these fishes depended on its availability and occasional cost. It was observed that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality; Ration; Taguchi; Tilapia; Yeast.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100011

Protein enrichment of cactus pear (Opuntia ficus - indica Mill) using Saccharomyces cerevisiae in solid-state fermentation
Provedor de dados: 52
Autores: Araújo,Lúcia de Fátima; Medeiros,Ariosvaldo Nunes; Perazzo Neto,Américo; Oliveira,Líbia de Sousa Conrado; Silva,Flávio Luiz Honorato da.
The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein enrichment; Solid-state fermentation; Cactus pear; Yeast.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400020

Growth of Nile tilapia Oreochromis niloticus fed with different levels of alcohol yeast
Provedor de dados: 57
Autores: MEDRI,V.; PEREIRA,G. V.; LEONHARDT,J. H.
Two hundred and forty 45-day-old fingerlings of Nile tilapia (Oreochromis niloticus), sexually reverted with an initial average weight of 1.25 ± 0.14 g, distributed in a totally casualized delineation, during 330 days. The effect of the substitution of 10%, 20% and 30% of the ration by distillery yeast (Saccharomiyces cerevisae) in balanced experimental rations on the development in the breeding of tilapias did not show a harmful effect up to the maximum tested level of 30%, showing that the choice of the yeast in the ration for these fishes depends on the availability and occasional cost. The yeast provides fish with good resistance to unfavorable environmental conditions, which could be verified since there were no statistical differences among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Oreochromis niloticus; Ration; Tilapia.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000100014

Genetic engineering of baker's and wine yeasts using formaldehyde hyperresistance-mediating plasmids
Provedor de dados: 56
Autores: Schmidt,M.; Cömer,A.; Grey,M.; Brendel,M.
Yeast multi-copy vectors carrying the formaldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker's and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO2 during fermentation, i.e., the selecting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Transformation; Hyperresistance to formaldehyde.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200004

Shared control of maltose and trehalose utilization in Candida utilis
Provedor de dados: 56
Autores: Rolim,M.F.; de Araujo,P.S.; Panek,A.D.; Paschoalin,V.M.F.; Silva,J.T.
Trehalose biosynthesis and its hydrolysis have been extensively studied in yeast, but few reports have addressed the catabolism of exogenously supplied trehalose. Here we report the catabolism of exogenous trehalose by Candida utilis. In contrast to the biphasic growth in glucose, the growth of C. utilis in a mineral medium with trehalose as the sole carbon and energy source is aerobic and exhibits the Kluyver effect. Trehalose is transported into the cell by an inducible trehalose transporter (K M of 8 mM and V MAX of 1.8 µmol trehalose min-1 mg cell (dry weight)-1. The activity of the trehalose transporter is high in cells growing in media containing trehalose or maltose and very low or absent during the growth in glucose or glycerol. Similarly, total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trehalose; Trehalase; Sugar transporter; Candida utilis; Yeast.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700002

Yeast CUP1 protects HeLa cells against copper-induced stress
Provedor de dados: 56
Autores: Xie,X.X.; Ma,Y.F.; Wang,Q.S.; Chen,Z.L.; Liao,R.R.; Pan,Y.C.
As an essential trace element, copper can be toxic in mammalian cells when present in excess. Metallothioneins (MTs) are small, cysteine-rich proteins that avidly bind copper and thus play an important role in detoxification. Yeast CUP1 is a member of the MT gene family. The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress. In this study, CUP1 expression was determined by quantitative real-time PCR, and copper content was detected by inductively coupled plasma mass spectrometry. Production of intracellular reactive oxygen species (ROS) was evaluated using the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) assay. Cellular viability was detected using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Overexpression; Copper stress; Viability; ROS.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700616

The biological effects of high-pressure gas on the yeast transcriptome
Provedor de dados: 56
Autores: Matsuoka,H.; Suzuki,Y.; Iwahashi,H.; Arao,T.; Suzuki,Y.; Tamura,K.
The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA microarray; Yeast; Hydrostatic pressure; Gas pressure; Nitrogen gas.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800016

Interdigital foot infections: Corynebacterium minutissimum and agents of superficial mycoses
Provedor de dados: 58
Autores: Sariguzel,Fatma Mutlu; Nedret Koc,A.; Yagmur,Gülhan; Berk,Elife.
Interdigital foot infections are mostly caused initially by dermatophytes, yeasts and less frequently by bacteria. Erythrasma caused by Corynebacterium minutissimum can be confused with superficial mycoses. The aim of the study was to determine the prevalence of the etiologic agents of superficial mycoses and the frequency of Corynebacterium minutissimum in interdigital foot infections. All the samples obtained from the 121 patients with interdigital foot infections were examined directly with the use of 20% potassium hydroxide mounts and Gram stain under the microscope and cultured on Sabouraud's dextrose agar plates. In identification of superficial mycoses, the rate was found to be 14% with the cultural method and 14% with direct microscopic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interdigital foot infections; Superficial mycoses; Dermatophytosis; Yeast; Corynebacterium minutissimum.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300003

Development and characterization of hybrids from native wine yeasts
Provedor de dados: 58
Autores: García,Verónica; Rivera,José; Contreras,Angela; Ganga,María Angélica; Martínez,Claudio.
For commercial purposes, the winemaking industry is constantly searching for new yeast strains. Historically, this has been achieved by collecting wild strains and selecting the best for industrial use through an enological evaluation. Furthermore, the increasing consumer demands have forced the industry to incorporate new strategies such as genetic engineering to obtain improved strains. In response to the lack of public acceptance of this methodology, alternative strategies based on breeding have gained acceptance in recent years. Through the use of conjugation of individual spores without the support of genetic engineering methods we generated intraspecific hybrids from wild strains with outstanding enological characteristics and interdelta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Hybrids; Wine.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200008

Analysis of the secondary compounds produced by Saccharomyces cerevisiae and wild yeast strains during the production of "cachaça"
Provedor de dados: 58
Autores: Dato,Maria Cecília Fachine; Pizauro Júnior,João Martins; Mutton,Márcia Justino Rossini.
The aim of this study is to compare the composition of "cachaças" produced in 10 fermentation cycles by Saccharomyces cerevisiae (Sc) and wild yeast strains [Pichia silvicola (Ps), Pichia anomala 1 (Pa1), Pichia anomala 2 (Pa2) and Dekkera bruxelensis (Db)], isolated from distilleries in Jaboticabal - SP, Brazil. The secondary components of the heart fraction were determined by gas chromatography. The levels of secondary components were influenced by the wine pH, which varied among yeast strains. S. cerevisiae showed slightly more secondary components, whereas wild strains produced more higher alcohols. Wild yeast strains were shown to be adequate for the production of a high quality "cachaça".
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distillate composition; Aldehyde; Higher alcohols; Secondary components; Yeast.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100014

Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate
Provedor de dados: 58
Autores: Accorsini,Fábio Raphael; Mutton,Márcia Justino Rossini; Lemos,Eliana Gertrudes Macedo; Benincasa,Maria.
Biosurfactants are bioactive agents that can be produced by many different microorganisms. Among those, special attention is given to yeasts, since they can produce many types of biosurfactants in large scale, using several kinds of substrates, justifying its use for industrial production of those products. For this production to be economically viable, the use of residual carbon sources is recommended. The present study isolated yeasts from soil contaminated with petroleum oil hydrocarbons and assessed their capacity for producing biosurfactants in low cost substrates. From a microbial consortium enriched, seven yeasts were isolated, all showing potential for producing biosurfactants in soybean oil. The isolate LBPF 3, characterized as Candida antarctica,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Biosurfactants; Glycerol; Soybean oil.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100013

Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris
Provedor de dados: 58
Autores: Zepeda,Andrea B.; Figueroa,Carolina A.; Pessoa,Adalberto; Farías,Jorge G.
ABSTRACT The growth of yeasts in culture media can be affected by many factors. For example, methanol can be metabolized by other pathways to produce ethanol, which acts as an inhibitor of the heterologous protein production pathway; oxygen concentration can generate aerobic or anaerobic environments and affects the fermentation rate; and temperature affects the central carbon metabolism and stress response protein folding. The main goal of this study was determine the implication of free fatty acids on the production of heterologous proteins in different culture conditions in cultures of Pichia pastoris. We evaluated cell viability using propidium iodide by flow cytometry and thiobarbituric acid reactive substances to measure cell membrane damage. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid peroxidation; Pyruvate pathway; Yeast.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400856

Antifungal activity of lectins against yeast of vaginal secretion
Provedor de dados: 58
Autores: Gomes,Bruno Severo; Siqueira,Ana Beatriz Sotero; Maia,Rita de Cássia Carvalho; Giampaoli,Viviana; Teixeira,Edson Holanda; Arruda,Francisco Vassiliepe Sousa; Nascimento,Kyria Santiago do; Lima,Adriana Nunes de; Souza-Motta,Cristina Maria; Cavada,Benildo Sousa; Porto,Ana Lúcia Figueiredo.
Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Sensitivity; Lectins.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200042

Statistical optimisation of cell growth and carotenoid production by Rhodotorula mucilaginosa
Provedor de dados: 58
Autores: Maldonade,Iriani R.; Rodriguez-Amaya,Delia B.; Scamparini,Adilma R. P.
Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 2(5-1) experimental design was used, and the variables were pH and the levels of glucose, yeast extract, MgSO4.7H2O and KH2PO4. The nitrogen source (yeast extract) was the most important variable in enhancing carotenoid production; MgSO4.7H2O and KH2PO4 had a negative influence. The initial pH had no significant effect on carotenoid and cell productions. We further investigated the effects of glucose and yeast extract effects, using a second-order central composite design (CCD) to optimise carotenoid production, which was adequately approximated with a full quadratic equation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fermentation; Torulene; Yeast; Response surface design; Rhodotorula mucilaginosa.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100012

Ethanol production in Brazil: a bridge between science and industry
Provedor de dados: 58
Autores: Lopes,Mario Lucio; Paulillo,Silene Cristina de Lima; Godoy,Alexandre; Cherubin,Rudimar Antonio; Lorenzi,Marcel Salmeron; Giometti,Fernando Henrique Carvalho; Bernardino,Claudemir Domingos; Amorim Neto,Henrique Berbert de; Amorim,Henrique Vianna de.
ABSTRACT In the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in Brazil. These contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. Nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. Better control of fermentation conditions can reduce the stress conditions for yeast cells and contamination by bacteria and wild yeasts. There are great research opportunities in production processes of the first-generation ethanol regarding high-value added products, cost reduction and selection of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Yeast; Fermentation; Distillery.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500064

Use of Pseudomonas stutzeri and Candida utilis in the improvement of the conditions of Artemia culture and protection against pathogens
Provedor de dados: 58
Autores: Abdelkarim,Mahdhi; Kamel,Chaieb; Fathi,Kammoun; Amina,Bakhrouf.
To evaluate the effect of two bacterial strains isolated from Artemia cysts and yeast (Candida utilis) on the survival, growth and total biomass production of its larvae, challenge tests were performed with Candida utilis, Pseudomonas stutzeri and Pasteurella haemolityca. In addition, a pathogenic strain of Vibrio alginolyticus was tested for comparative purposes. Pseudomonas stutzeri and Candida utilis have no impact on survival, but enhance growth and total biomass production of the larvae. However, we noted that Pasteurella haemolityca affect negatively Artemia larvae. The adhesion and antagonism assay demonstrates that Candida utilis and Pseudomonas stutzeri are fairly adherent and play an important role in the enhancement of the protection of Artemia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemia; Probiotics; Adhesion; Yeast; Bacteria.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100017

Chlorine dioxide against bacteria and yeasts from the alcoholic fermentation
Provedor de dados: 58
Autores: Meneghin,Silvana Perissatto; Reis,Fabricia Cristina; Almeida,Paulo Garcia de; Ceccato-Antonini,Sandra Regina.
The ethanol production in Brazil is carried out by fed-batch or continuous process with cell recycle, in such way that bacterial contaminants are also recycled and may be troublesome due to the substrate competition. Addition of sulphuric acid when inoculum cells are washed can control the bacterial growth or alternatively biocides are used. This work aimed to verify the effect of chlorine dioxide, a well-known biocide for bacterial decontamination of water and equipments, against contaminant bacteria (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides) from alcoholic fermentation, through the method of minimum inhibitory concentration (MIC), as well as its effect on the industrial yeast inoculum. Lower MIC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorine dioxide; Bacteria; Yeast; Antibacterial agent; Alcohol; Fermentation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200026

Mycological contamination in dental unit waterlines in Istanbul, Turkey
Provedor de dados: 58
Autores: Kadaifciler,Duygu Göksay; Ökten,Suzan; Sen,Burhan.
Studies on dental units (DUs) are conducted either for the prevention or the reduction of the density of bacterial contamination in dental unit waterlines (DUWLs). However, the existence of fungi in the these systems requires more attention. During dental treatment, direct contact with water contaminated with fungi such as Candida, Aspergillus, or inhalation of aerosols from high-speed drill may cause various respiratory infections, such as asthma, allergies, and wounds on mucose membranes, especially on immunocompromised patients and dentists. The aims of this study are to investigate the number and colonization of fungi in DUWLs in the city of Istanbul, Turkey. Water samples were collected from air-water syringes, high-speed drills, and inlet waters from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental unit waterlines; Dental equipments; Filamentous fungi; Mycological contamination; Yeast.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300049

Occurrence of killer Candida glabrata clinical isolates
Provedor de dados: 58
Autores: O,Arroyo-Helguera; Alejandro,De Las Penas; Irene,Castaño.
In this work we characterized the occurrence of killer activity in 64 Candida glabrata clinical isolates under different conditions. We found that only 6.25 % of the clinical isolates tested were positive for killer activity against a Saccharomyces cerevisiae W303 sensitive strain. Sensitivity of killer activity to different values of pH and temperatures was analyzed. We found that the killer activity presented by all isolates was resistant to every pH and temperature tested, although optimal activity was found at a range of pH values from 4 to 7 and at 37ºC. We did not observe extrachromosomal genetic elements associated with killer activity in any of the positive C. glabrata isolates. The killer effect was due to a decrease in viability and DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida glabrata; Killer activity; Yeast; Saccharomyces.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300005

Hyphal-like extension and pseudohyphal formation in industrial strains of yeasts induced by isoamyl alcohol
Provedor de dados: 58
Autores: Ceccato-Antonini,Sandra Regina; Silva,Paula Cristina da.
Yeasts can produce pseudohyphae and hyphal-like extensions under certain growth conditions like isoamyl alcohol (IAA) induction, a chief constituent of fusel oil, which is a subproduct from the ethanolic fermentation. The morphology switch from yeast to a filamentous form can be troublesome to the process. In this work it was studied the influence of fusel alcohols, nitrogen sources (ammonium sulphate and leucine) and glifosate (a chemical maturator for sugar cane) added to a complex medium on some industrial strains of yeasts isolated from the fermentative process. Two industrial strains showed transition to hyphal-like extensions or pseudohyphae (clusters of cells) upon addition of IAA from 0.3 to 0.9% /v. The alterations were reversible when the yeasts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoamyl alcohol; Ethanol; Yeast; Pseudohyphae; Hyphal-like extension.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300004

Fermentation process for production of apple-based kefir vinegar: microbiological, chemical and sensory analysis
Provedor de dados: 58
Autores: Viana,Roberta Oliveira; Magalhães-Guedes,Karina Teixeira; Braga Jr.,Roberto Alves; Dias,Disney Ribeiro; Schwan,Rosane Freitas.
Abstract The aim of this study was to develop a kefir apple-based vinegar and evaluate this fermentation process using new methodology with Biospeckle Laser. Brazilian kefir grains were inoculated in apple must for vinegar production. In this study, the microbial community present in kefir, and correspondent vinegar, was investigated using Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) technique. Saccharomyces cerevisiae, Lactobacillus paracasei, Lactobacillus plantarum, Acetobacter pasteurianus and Acetobacter syzygii were the microbial species identified. S. cerevisiae, L. plantarum, A. pasteurianus and A. syzygii were found in smaller quantities at the beginning of the alcoholic fermentation, but were found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Saccharomyces; Lactobacillus; Apple; MALDI-TOF; Biospeckle Laser; Vinegar.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300592

Identification of genes expressed by Cryptococcus gattii during iron deprivation
Provedor de dados: 58
Autores: Paula,Daphine Ariadne Jesus de; Silva,Lívia Kmetzsch Rosa e; Staats,Charley Christian; Vainstein,Marilene H.; Joanoni,Ana Lúcia Pinto; Nakazato,Luciano; Dutra,Valéria.
Cryptococcus neoformans and C. gattii are pathogenic yeasts that cause life-threatening diseases in humans and animals. Iron is an essential nutrient for virtually every organism as it functions as a cofactor in numerous essential enzymatic reactions. In the literature, the competition for iron between microbes and mammalian hosts during infection is well documented. In this study, we used representational difference analysis (RDA) in order to gain a better understanding of how C. gattii responds to iron starvation. A total of 15 and 29 genes were identified as having altered expression levels due to iron depletion after 3 h and 12 h, respectively. Of these, eight genes were identified in both libraries. The transcripts were related to many biological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcus gattii; Iron; Representational difference analysis; Yeast.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300008

Recombinants proteins for industrial uses: utilization of Pichia pastoris expression system
Provedor de dados: 58
Autores: Rabert,Claudia; Weinacker,Daniel; Pessoa Jr,Adalberto; Farías,Jorge G.
The innovation in industrial process with impact in the efficient production is the major challenge for actual industry. A high numerous of enzymes are utilized in at different level of process; the search for new alternatives with better characteristic has become a field of study of great interest, the recombinant protein achievement in a different host system is an alternative widely assessed for production of this. The microorganism Pichia pastoris has been used like a successful expression system in diverse areas, improved the yield and extraction-recovery of the product expressed. The reported of diverse authors in the production of enzymes with different application in industry is varied, in this review the different industry areas and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant protein; Protein production; Yeast; Industrial applications.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200002

The uptake of different iron salts by the yeast Saccharomyces cerevisiae
Provedor de dados: 58
Autores: Gaensly,Fernanda; Picheth,Geraldo; Brand,Debora; Bonfim,Tania M.B.
Yeasts can be enriched with microelements, including iron; however, special physicochemical conditions are required to formulate a culture media that promotes both yeast growth and iron uptake. Different iron sources do not affect biomass formation; however, considering efficacy, cost, stability, and compatibility with Saccharomyces cerevisiae metabolism, ferrous sulphate is recommended.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Saccharomyces cerevisiae; Iron uptake; Iron sources; Biomass formation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200016

Isolation and characterization of yeasts from fermented apple bagasse as additives for ruminant feeding
Provedor de dados: 58
Autores: Castillo-Castillo,Y.; Ruiz-Barrera,O.; Burrola-Barraza,M.E.; Marrero-Rodriguez,Y.; Salinas-Chavira,J.; Angulo-Montoya,C.; Corral-Luna,A.; Arzola-Alvarez,C.; Itza-Ortiz,M.; Camarillo,J.
Abstract Solid-state fermentation can be used to produce feeds for ruminants, which can provide an enriched population of yeasts to improve ruminal fermentation. Fermentation of apple bagasse was performed to obtain a yeast-rich product, with the objective of isolating, identifying, and characterizing yeast strains and testing their capability to enhance in vitro ruminal fermentation of fibrous feeds. Yeasts were isolated from apple bagasse fermented under in vitro conditions, using rumen liquor obtained from cannulated cows and alfalfa as a fibrous substrate. A total of 16 new yeast strains were isolated and identified by biochemical and molecular methods. The strains were designated Levazot, followed by the isolate number. Their fermentative capacity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple bagasse; Ruminal; Fermentation; Yeast.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400889

Applications of recombinant Pichia pastoris in the healthcare industry
Provedor de dados: 58
Autores: Weinacker,Daniel; Rabert,Claudia; Zepeda,Andrea B.; Figueroa,Carolina A.; Pessoa,Adalberto; Farías,Jorge G.
Since the 1970s, the establishment and development of the biotech industry has improved exponentially, allowing the commercial production of biopharmaceutical proteins. Nowadays, new recombinant protein production is considered a multibillion-dollar market, in which about 25% of commercial pharmaceuticals are biopharmaceuticals. But to achieve a competitive production process is not an easy task. Any production process has to be highly productive, efficient and economic. Despite that the perfect host is still not discovered, several research groups have chosen Pichia pastoris as expression system for the production of their protein because of its many features. The attempt of this review is to embrace several research lines that have adopted Pichia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Heterologous expression system; Recombinant protein; Production; Scale up.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400004

Mobilization of endogenous glycogen and trehalose of industrial yeasts
Provedor de dados: 58
Autores: Paulillo,Silene Cristina de Lima; Yokoya,Fumio; Basso,Luiz Carlos.
The fermentation of yeast reserve carbohydrates, glycogen and trehalose is a procedure to increase protein level of yeast cells and improve ethanol production. This work studied on the degradation kinetics of glycogen and trehalose carried out with two industrial strains of Saccharomyces cerevisiae (PE-2 and SA-1) and the effect of different temperatures (38º, 40º, 42º and 44ºC) on degradation rate. Endogenous fermentation was carried out in a yeast suspension at 20% (w/v) based on wet weight, suspended in fermented media with 3.0 to 4.5 % (v/v) of ethanol. The degradation of the carbohydrate reserves at 40ºC followed first-order kinetics, showing that its rate is mainly dependent on the carbohydrate concentration in the cell. The degradation rate (k)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Glycogen; Trehalose; Yeast; Fermentation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300014

Isolation and characterization of phenol degrading yeasts from wastewater in the coking plant of Zarand, Kerman
Provedor de dados: 58
Autores: Karimi,Maryam; Hassanshahian,Mehdi.
Abstract Phenol and phenolic compounds are environmental pollutants present in industrial wastewaters such as coal tar, oil refineries and petrochemical plants. Phenol removal from industrial effluents is extremely important for the protection of environment. Usually, phenol degradation is carried out by physicochemical methods that are costly and produce hazardous metabolites. Recently, phenol biodegradation has been considered. Yeasts are the most important phenol biodegraders. In this study, the phenol-degrading yeast from environmental samples (soil and wastewater) was isolated from the coking plant of Zarand, Kerman. Then total heterotrophic yeasts were counted. The soil samples had higher rates of yeast degrader, in comparison to wastewater samples....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Coal tar; Coking plant; Yeast; Zarand.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100018

Yeasts isolated from sand and sea water in beaches of Olinda, Pernambuco state, Brazil
Provedor de dados: 58
Autores: Loureiro,Silvia Tereza Azedo; Cavalcanti,Maria Auxiliadora de Queiroz; Neves,Rejane Pereira; Passavante,José Zanon de Oliveira.
The aim of this work was to isolate and identify yeasts from sand and sea water collected in two beaches of Olinda, Pernambuco state, Brazil. Thirty two samples of both sand and water in both beaches were obtained in the dry (December 2000 and February 2001) and rainy (June and July 2001) seasons. Two hundred and ninety two strains of yeast were obtained, and they belonged to four genera and 31 species. Candida was the most prevalent genus. Candida catenulata, C. fenica, C. sake, Brettanomyces bruxelenses and Rhodotorula mucilaginosa were the most commonly found species in both beaches Bairro Novo and Casa Caiada.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Sand; Water; Beaches.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400005

Oxidative stress and antioxidant response in a thermotolerant yeast
Provedor de dados: 58
Autores: Mejía-Barajas,Jorge A.; Montoya-Pérez,Rocío; Salgado-Garciglia,Rafael; Aguilera-Aguirre,Leopoldo; Cortés-Rojo,Christian; Mejía-Zepeda,Ricardo; Arellano-Plaza,Melchor; Saavedra-Molina,Alfredo.
Abstract Stress tolerance is a key attribute that must be considered when using yeast cells for industrial applications. High temperature is one factor that can cause stress in yeast. High environmental temperature in particular may exert a natural selection pressure to evolve yeasts into thermotolerant strains. In the present study, three yeasts (Saccharomyces cerevisiae, MC4, and Kluyveromyces marxianus, OFF1 and SLP1) isolated from hot environments were exposed to increased temperatures and were then compared with a laboratory yeast strain. Their resistance to high temperature, oxidative stress, and antioxidant response were evaluated, along with the fatty acid composition of their cell membranes. The SLP1 strain showed a higher specific growth rate,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Increased temperature; Oxygen-derived free radicals; Thermotolerant.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200326

Yeast biomass production: a new approach in glucose-limited feeding strategy
Provedor de dados: 58
Autores: Vieira,Érika Durão; Andrietta,Maria da Graça Stupiello; Andrietta,Silvio Roberto.
The aim of this work was to implement experimentally a simple glucose-limited feeding strategy for yeast biomass production in a bubble column reactor based on a spreadsheet simulator suitable for industrial application. In biomass production process using Saccharomyces cerevisiae strains, one of the constraints is the strong tendency of these species to metabolize sugars anaerobically due to catabolite repression, leading to low values of biomass yield on substrate. The usual strategy to control this metabolic tendency is the use of a fed-batch process in which where the sugar source is fed incrementally and total sugar concentration in broth is maintained below a determined value. The simulator presented in this work was developed to control molasses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Biomass; Fed-batch; Process simulation; Saccharomyces cerevisiae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200035

Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology
Provedor de dados: 58
Autores: Faria,Janaína T. de; Rocha,Pollyana F.; Converti,Attilio; Passos,Flávia M.L.; Minim,Luis A.; Sampaio,Fábio C.
The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under different conditions of ethanol concentration, treatment time and temperature according to a central composite rotatable design (CCRD), and the collected results were then worked out by response surface methodology (RSM). Cell permeabilization was improved by an increase in ethanol concentration and simultaneous decreases in the incubation temperature and treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Cheese whey; Permeabilization; Ethanol; Β-galactosidase.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400007

Influence of fermentation time on characteristics of sourdough bread
Provedor de dados: 60
Autores: Aplevicz,Krischina Singer; Ogliari,Paulo José; Sant'Anna,Ernani Sebastião.
Sourdough is used in the manufacture of numerous baked products. The microorganisms used in this preparation of sourdoughs included two strains from the Lactobacillus paracasei (1 and 2) and two strains from the Saccharomyces cerevisiae group (1 and 2). Samples of raw dough were analyzed for pH, titratable acidity and plate counts and samples of resulting bread were analyzed for pH, titratable acidity and specific volume. The samples were analyzed every 2 h, between 4 and 10 h of fermentation. After 10 hours of fermentation, the lowest values of pH were for dough with LC2 and bread with SC1. Titratable acidity values increased over time, with the highest levels of acidity were found in the dough and bread with yeasts. Lactic acid bacteria showed the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sourdough; Bread; Lactic acid bacteria; Yeast; Linear regression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200005

Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation
Provedor de dados: 118
Autores: Herrera,Esperanza; Murillo,Manuel; Berumen,Leslie; Soto-Cruz,Nicolas O.; Páez-Lerma,Jesús B.
Abstract Opuntia ficus-indica is used in animal feed but has a low protein content (above 4%) in comparison to high-quality forage (above 16%). It is necessary to develop technologies that improve the nutrimental value of Opuntia ficus-indica through the process of solid-state fermentation (SSF). Therefore, the objective of this study was to evaluate the changes in the chemical composition of Opuntia ficus-indica during the SSF with the yeast Kluyveromyces marxianus ITD00262. Kluyveromyces marxianus ITD00262 was grown in glucose, peptone, yeast (GPY) broth, at 28 °C and pH 4.8 for 12 h. Opuntia ficusindica was incubated in SSF at 28 °C without inoculation (T1) or inoculated with K. marxianus ITD00262 (T2). Fermented samples of Opuntia ficus-indica were...
Tipo: Journal article
Palavras-chave: Fermentation; Nutritional quality; Protein; Opuntia; Yeast.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000200113

RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma
Provedor de dados: 64
Autores: Rios,Danielle Alves da Silva; Borba,Thais de Matos de; Kalil,Susana Juliano; Burkert,Janaína Fernandes de Medeiros.
The interest in carotenoid production from natural sources has increased based in their possible use as dyes and their powerful biological antioxidant capacity. This study evaluated the use of rice parboiling wastewater (RPW) as an alternative substrate for the bioproduction of carotenoids using the yeast Phaffia rhodozyma and found it to be promising as the only source of nutrients, reaching a concentration of 0.6 μg mL-1 (259.1 μg g-1) in 48 h. To increase the potential use of this industrial effluent, a study of supplementation was carried out to enhance the production of carotenoids using the methodology of experimental design (a 2IV 6-2 fractional factorial design sequenced by a 24 central composite design). The conditions for maximizing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodye; Submerged cultive; Response surface methodology; Yeast.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000400401