Consulta: creatorFacets:"Boudry, Pierre"
Registros recuperados: 273
Data/hora: 10/06/2024 10:25:45

Effects of age and environment on the summer mortality in cupped oyster Crassostrea gigas during the first two years
Provedor de dados: 5
Autores: Degremont, Lionel; Boudry, Pierre; Soletchnik, Patrick; Bedier, Edouard; Ropert, Michel; Samain, Jean-francois.
Three successive generations were produced between 2001 and 2003 to assess to what extent genetic variability exists for survival in juvenile oysters Crassostrea gigas. For each generation, two groups were selected for their high ("R" for resistant) and low ("S" for susceptible) survival. Significant differences in mortality were observed during the first year in Riviere d'Auray (RA) (Brittany-France). However, low and similar mortality were observed for both groups, with no significant difference between groups in the first and second generation in this site, where the first summer is the critical, sensitive period with a clear age effect. In Baie des Veys (BDV) (Normandie-France), where mortalities usually mainly affect adults, mortality between groups,...
Tipo: Text
Palavras-chave: Crassostrea gigas; Spat; Spatial variations; Survival; Rearing; Mollusc culture; Marine molluscs; Environmental factors; Nursery grounds; Mortality causes; Biological age; Aquaculture techniques.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3385.pdf

A preliminary genetic linkage map for the European flat oyster Ostrea edulis L.
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Harley, Crhis; Boudry, Pierre; Heurtebise, Serge; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic to European coasts, both Atlantic and Mediterranean. It has been exploited since Roman times, but its aquaculture production decreased from around 20000 tons in the 1950's to 1500 tons today because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has been undertaking a breeding program to produce families of oysters tolerant to Bonamia. In this context, a further step would be to identify QTLs for resistance to this parasite. Therefore, as a first step a genetic map is now being built. The establishment of a genetic linkage map will provide a foundation for the mapping of QTLs, with the ultimate objective to implement...
Tipo: Text
Palavras-chave: QTLs; Ostrea edulis; Flat oyster; Genetic linkage map; Genetic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3466.pdf

Generation and analysis of a 29,745 unique Expressed Sequence Tags from the Pacific oyster (Crassostrea gigas) assembled into a publicly accessible database: the GigasDatabase
Provedor de dados: 5
Autores: Fleury, Elodie; Huvet, Arnaud; Lelong, Christophe; De Lorgeril, Julien; Boulo, Viviane; Gueguen, Yannick; Bachere, Evelyne; Tanguy, Arnaud; Moraga, Dario; Fabioux, Caroline; Lindeque, Penelope; Shaw, Jenny; Reinhardt, Richard; Prunet, Patrick; Davey, Grace; Lapegue, Sylvie; Sauvage, Christopher; Corporeau, Charlotte; Moal, Jeanne; Gavory, Frederick; Wincker, Patrick; Moreews, Francois; Klopp, Christophe; Mathieu, Michel; Boudry, Pierre; Favrel, Pascal.
Background: Although bivalves are among the most-studied marine organisms because of their ecological role and economic importance, very little information is available on the genome sequences of oyster species. This report documents three large-scale cDNA sequencing projects for the Pacific oyster Crassostrea gigas initiated to provide a large number of expressed sequence tags that were subsequently compiled in a publicly accessible database. This resource allowed for the identification of a large number of transcripts and provides valuable information for ongoing investigations of tissue-specific and stimulus-dependant gene expression patterns. These data are crucial for constructing comprehensive DNA microarrays, identifying single nucleotide...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6770.pdf

High-density genetic map and identification of QTLs for responses to temperature and salinity stresses in the model brown alga Ectocarpus
Provedor de dados: 5
Autores: Avia, Komlan; Coelho, Susana M.; Montecinos, Gabriel J.; Cormier, Alexandre; Lerck, Fiona; Mauger, Stephane; Faugeron, Sylvain; Valero, Myriam; Cock, J. Mark; Boudry, Pierre.
Deciphering the genetic architecture of adaptation of brown algae to environmental stresses such as temperature and salinity is of evolutionary as well as of practical interest. The filamentous brown alga Ectocarpus sp. is a model for the brown algae and its genome has been sequenced. As sessile organisms, brown algae need to be capable of resisting the various abiotic stressors that act in the intertidal zone (e.g. osmotic pressure, temperature, salinity, UV radiation) and previous studies have shown that an important proportion of the expressed genes is regulated in response to hyposaline, hypersaline or oxidative stress conditions. Using the double digest RAD sequencing method, we constructed a dense genetic map with 3,588 SNP markers and identified 39...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00377/48779/49186.pdf

A first genetic linkage map of the European flat oyster, Ostrea edulis : towards the identification of Bonamia resistance QTLs
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Beaumont, Andy.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, the next step is to identify QTLs for resistance to this parasite. A genetic map is therefore being established as a basis for QTL work. The reference family is an F2 family produced by single pair mating of two oysters from the same F1 family. The F1 family was itself produced from a bi-parental cross between a wild oyster and an individual from the...
Tipo: Text
Palavras-chave: Genetic linkage map; Parasite; Genetic; QTLs; Resistance; Bonamia ostreae; Ostrea edulis; Flat oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3464.pdf

Selective breeding to improve resistance against summer mortality in the Pacific oyster Crassostrea gigas : results after 3 generations
Provedor de dados: 5
Autores: Boudry, Pierre; Degremont, Lionel; Bedier, Edouard; Samain, Jean-francois.
Summer mortality of adults and juveniles has been reported in the Pacific oyster, Crassostrea gigas, for many years and in several countries. The French multidisciplinary program "Morest" aims to investigate the causes of the summer mortality in Crassostrea gigas. Within this program, we designed multi-site field experiments to assess to what extent genetic variability exists for summer mortality in French populations of C. gigas and to determine whether selective breeding could improve survival.
Tipo: Text
Palavras-chave: Growth; Resistance; Selective breeding; Genetic; Crassostrea gigas; Pacific oyster; Summer mortality.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3448.pdf

The identification of genes from the oyster Crassostrea gigas that are differentially expressed between progeny exhibiting opposed susceptibility to summer mortality
Provedor de dados: 5
Autores: Huvet, Arnaud; Degremont, Lionel; Labreuche, Yannick; Daniel, Jean-yves; Dubrunfaut, Thomas; Boudry, Pierre; Haure, Joel; Bedier, Edouard; Ropert, Michel; Herpin, Amaury; Cunningham, Charles; Moal, Jeanne; Samain, Jean-francois.
Summer mortality associated with juveniles of the oyster Crassostrea gigas is the result of a complex interaction between the host, pathogens and environment. Divergent selection based on summer survival have been applied to produce families with resistant and susceptible progeny. Here, we describe the use of suppression subtractive hybridization to generate 150 clones that were differentially regulated between resistant and susceptible F2 progeny. The nucleotide sequence of these 150 clones was determined. Among the 25 unique sequences that matched with the products of known genes, those putatively implicated in energy generation (12%) and immune function (16%), systems suspected to be implicated in summer mortality, were extended and analyzed by real...
Tipo: Text
Palavras-chave: Selection; Gene; Genetic; Summer mortality; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3447.pdf

Conséquences génétiques de la production de larves d'huîtres en écloserie : étude des processus de dérive et de sélection
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Batista, Frederico; Baron, Sophie; Ernande, Bruno; Haffray, Pierrick; Boudry, Pierre.
Previous studies have shown heritable variation in larval developmental traits in the Pacific oyster Crassostrea gigas. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors, specific to hatcheries, were examined: the effect of discarding the smallest larvae (i.e. culling) and the effect of temperature (20°C versus 26°C). A mixed-family approach was used in order to infer the genetic composition of larval populations and family assignment, limiting possible environmental bias and allowing the study of a relatively large number of families using a limited number of larval tanks. Our results show that three multiplexed highly polymorphic microsatellite markers are a powerful tool for family assignment and,...
Tipo: Text
Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-1505.pdf

Genetic basis of summer mortality in juvenile cupped oysters
Provedor de dados: 5
Autores: Degremont, Lionel; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Soletchnik, Patrick; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois.
The French project "Morest", coordinated by IFREMER, aims to understand the causes of the summer mortalities in Crassostrea gigas. In 2001, three sets of families were bred following a nested half-sib mating design. 17 half-sib families (HSF) were obtained in this first generation (G1) and reared in 3 sites. Significant differences in survival were observed among HSF, and some HSF showed high levels of mortality in all sites, clearly indicating a genetic basis for survival. In 2002, a second generation (G2), including divergent selection and inbred lines, was constituted. Monitoring of survival and growth of G2s were the same as in 2001. Significant differences in survival were found between the offspring of the "high" and "low" selected groups and between...
Tipo: Text
Palavras-chave: Summer; Oyster culture; Juveniles mortality; Genetics; Commercial species; Breeding.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3346.pdf

RAD sequencing sheds new light on the genetic structure and local adaptation of European scallops and resolves their demographic histories
Provedor de dados: 5
Autores: Vendrami, David L. J.; De Noia, Michele; Telesca, Luca; Handal, William; Charrier, Gregory; Boudry, Pierre; Eberhart-phillips, Luke; Hoffman, Joseph I.
Recent developments in genomics are advancing our understanding of the processes shaping population structure in wild organisms. In particular, reduced representation sequencing has facilitated the generation of dense genetic marker datasets that provide greater power for resolving population structure, investigating the role of selection and reconstructing demographic histories. We therefore used RAD sequencing to study the great scallop Pecten maximus and its sister species P. jacobeus along a latitudinal cline in Europe. Analysis of 219 samples genotyped at 82,439 single nucleotide polymorphisms clearly resolved an Atlantic and a Norwegian group within P. maximus as well as P. jacobeus, in support of previous studies. Fine-scale structure was also...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00497/60901/64240.pdf

The use of microsatellite markers for parentage analysis in the Pacific cupped oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Kotoulas, Georgios; Magoulas, Antonio; Hervouet, Veronique; Bonhomme, Francois; Gerard, Andre.
Highly polymorphic genetic markers can be useful tools for the analysis of parental contributions. The present paper reports 3 experiments using microsatellite markers to analyse parental contributions in controlled in vitro factorial crosses of the cupped oyster Crassostrea gigas. Parentage analysis was eased by the large polymorphism observed at the 3 loci studied. In a cross between 20 males and 20 females, a quarter of the progeny could be assigned to a given parent using a single microsatellite locus showing 28 alleles. In 3 crosses between 5 males and females, all the progeny could be assigned to a family by using 3 loci. In all cases, the combined genotyping at 2 loci allowed parentage to be detennined unambiguously. Despite the balanced gametic...
Tipo: Text
Palavras-chave: Crassostrea gigas; Microsatellites; Parental contributions; Crassostrea gigas; Microsatellites; Contributions parentales.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/acte-2739.pdf

Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana; Milan, Massimo; Guo, Xinmei; Li, Li; Zhang, Guofan; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.
AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism...
Tipo: Text
Palavras-chave: Marine bivalve; Crassostrea gigas; Hypoxia; AMP-activated protein kinase; Alternative splicing; Adductor muscle.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00119/22998/21397.pdf

Projet CRYOYSTER: Optimisation, standardisation et validation de la congélation de laitance d'huître creuse Crassostrea gigas à des fins de conservation et de diffusion génétique - Appel d'offre de l'OFIMER du 8 Novembre 2001 - Rapport final
Provedor de dados: 5
Autores: Brizard, Raphael; Bernardi, Maud; Boudry, Pierre; Haffray, Pierrick; Labbe, Catherine; Maisse, Gérard; Maurouard, Elise; Robert, Rene; Roger, Jean-luc.
Afin d'appuyer la filière ostréicole, les programmes de recherche actuels visent à proposer des méthodes d'amélioration génétique des rendements et de la qualité du produit (sélection, polyploïdisation). A l'image des productions animales terrestres, la continuation de ces travaux et la diffusion du progrès génétique dans la filière seraient grandement facilitée par le développement de techniques de conservation des génotypes sélectionnés. La cryopréservation qui est utilisée fréquemment dans les programmes de sélection des animaux terrestres et des espèces piscicoles n'est toujours pas disponible pour les mollusques bivalves. Cette technique présente les atouts qui peuvent pourraient satisfaire les différents acteurs de la filière ostréicole car elle...
Tipo: Text
Palavras-chave: Congélation sperme; Standardisation; Procédure; Ecloserie expérimentale; Fécondation; Cryopréservation; Sperme cryopréservé.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00049/16056/13516.pdf

Effect of age and environment on the summer mortality in cupped oyster Crassostrea gigas during the first two years
Provedor de dados: 5
Autores: Degremont, Lionel; Boudry, Pierre; Soletchnik, Patrick; Bedier, Edouard; Ropert, Michel; Samain, Jean-francois.
Three successive generations were produced between 2001 and 2003 to assess to what extent genetic variability exists for this trait in juvenile oysters Crassostrea gigas.  For each generation, two groups were selected for their high ("R" for resistant) and low ("S" for susceptible) survival.  Significant differences of mortality were observed during the first year in Rivière d'Auray (RA) (Brittany-France).  However, low and similar mortality were observed for both groups, with no significant difference between groups in the first and second generation in this site, where the first summer is the critical sensitive period with a clear age effect.  In Baie des Veys (BDV) (Normandie-France), where mortalities usually mainly affect adults, mortality between...
Tipo: Text
Palavras-chave: Genetic variability; Genetic; Summer mortality; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3442.pdf

A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Taris, Nicolas; Baron, S; Sharbel, Tim; Sauvage, Christopher; Boudry, Pierre.
The analysis of parentage using microsatellite markers is of increasing importance, notably in aquaculture genetic research where communal rearing of mixed families can be used to reduce unwanted environmental variance. We present here an optimization of parental genotype assessment for larvae or adults of the Pacific oyster, Crassostrea gigas, using a multiplex system of three microsatellite loci. In conjunction with a simple DNA extraction protocol, this method enables high throughput analyses of parentage in C. gigas. Using this method, we successfully determined the parentage of 93% (1224 out of 1318) of the progeny in a factorial cross between 3 females and 10 males. The inability to genotype the remaining 7% was due to DNA degradation of larvae...
Tipo: Text
Palavras-chave: Oysters; Aquaculture; Parental assignment; High throughput; Larval DNA extraction; Multiplex.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1814.pdf

In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Fleury, Elodie; Corporeau, Charlotte; Quillien, Virgile; Daniel, Jean-yves; Riviere, Guillaume; Boudry, Pierre; Fabioux, Caroline.
We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated...
Tipo: Text
Palavras-chave: Marine bivalve; Crassostrea gigas; Reproduction; RNA interference; Transforming growth factor-beta.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19794/17486.pdf

Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Jeffroy, Fanny; Daniel, Jean-yves; Quere, Claudie; Le Souchu, Pierrick; Van Wormhoudt, A.; Boudry, Pierre; Moal, Jeanne; Samain, Jean-francois.
In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values...
Tipo: Text
Palavras-chave: Alpha-amylase; Crassostrea gigas; Digestive enzyme; Michaelis-Menten constant; Regulation.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19869/17521.pdf

Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc.
In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potential (MMP), and intracellular ATP content in relation to motion parameters and 2) the involvement of OXPHOS for spermatozoal movement using carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a...
Tipo: Text
Palavras-chave: Crassostrea gigas; Intracellular ATP content; Mitochondrial membrane potential; Oxidative phosphorylation; Sperm motility.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00303/41376/40666.pdf

Possibilities to prevent Crassostrea gigas oyster summer mortality in France
Provedor de dados: 5
Autores: Samain, Jean-francois; Ropert, Michel; Bedier, Edouard; Soletchnik, Patrick; Mazurie, Joseph; Le Coz, Florence; Blin, Jean-louis; Mille, Dominique; Pajot, Régis; Haffray, Patrick; Bacher, Cedric; Grangere, Karine; Grangere, Karine; Pouvreau, Stephane; Bourles, Yves; Gohin, Francis; Woerther, Patrice; Boudry, Pierre.
Summer mortality has been reported in the Pacific oyster, Crassostrea gigas, for many years in Japan, US and Europe. It affects both adults and juveniles and the causes of this phenomenon are complex. Environmental, physiological or pathological putative causes were studied all over the world. Recently, the MOREST project ((MORtalitEStivale) a multidisciplinary project (2001-2005) focused on his problem led to a better understanding of this phenomenon in France. The results showed in these geographical conditions, the main interactions between the oysters, their environment and certain pathogens.
Tipo: Text
Palavras-chave: Interaction model; Environmental parameters; Summer mortality; Crassostrea gigas; Pacific oyster.
Ano: 2006
URL: https://archimer.ifremer.fr/doc/2006/acte-3476.pdf

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles following its introduction for aquaculture production
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andrew; King, J. W.; Turner, J. R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, has been translocated widely for the purpose of aquaculture and is among the most important cultured shellfish. C. gigas has a complex history of introductions in Europe. In France, massive introduction was performed in the early 1970’s, allowing massive spatfall in favourable areas to become the basis of the local oyster industry. In the U.K, hatchery propagation was used to supply local production. Some seed was also exported to Northern Europe were demographically independent populations have been reported in the last decades. In the present study 22 sites were sampled in the British Isles, Denmark, France and Spain in order to assess the genetic diversity and population differentiation...
Tipo: Text
Palavras-chave: Invasion genetics; Pacific oyster; Crassostrea gigas; British Isles.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27814/26007.pdf

Etude transcriptomique, génétique et fonctionnelle de la gamétogenèse chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Bigot, L.; Dubos, M. P.; Dheilly, N.; Favrel, P.; Kellner, K.; Lelong, C.; Martinez, A. S.; Riviere, G.; Santerre, C.; Sourdaine, P.; Degremont, Lionel; Flahauw, Emilie; Hatt, Philippe-jacques; Heurtebise, Serge; Lapegue, Sylvie; Azzouzi, N.; Galibert, F.; Boudry, Pierre; Corporeau, Charlotte; Fabioux, Caroline; Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana.
Le projet GAMETOGENES vise à améliorer les connaissances des bases physiologiques et génétiques de la reproduction et des métabolismes associés chez un mollusque d'importance économique l'huître creuse, Crassostrea gigas. Il a pour objectifs (1) la définition de marqueurs clés de la gamétogenèse, de l'investissement reproducteur et du déterminisme du sexe chez une espèce hermaphrodite à protandrie irrégulière (2) la recherche de QTL de survie aux mortalités estivales ou à l'effort de reproduction dont le succès repose sur l'augmentation de la densité de marqueurs sur les cartes génétiques existantes (SNP en particulier) et sur les liens qui pourront être établis entre ces cartes de liaison et une carte physique (3) le développement d'outils pour valider la...
Tipo: Text
Palavras-chave: Génétique; Huître creuse; Crassostrea gigas; Gamétogenèse; QTL; Marqueur; Physiologie.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00107/21781/19355.pdf

Rapport annuel 2017 de l’unité Physiologie Fonctionnelle des Organismes Marins (PFOM)
Provedor de dados: 5
Autores: Boudry, Pierre; Fleury, Elodie; Le Roux, Frederique; Zambonino, Jose-luis.
Tipo: Text
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00451/56298/57880.pdf

Ostreid herpes virus 1 infection in families of the Pacific oyster, Crassostrea gigas, during a summer mortality outbreak: Differences in viral DNA detection and quantification using real-time PCR
Provedor de dados: 5
Autores: Sauvage, Christopher; Pepin, Jean-francois; Lapegue, Sylvie; Boudry, Pierre; Renault, Tristan.
Ostreid herpes virus 1 (OsHV-1) infections, notably reported in Europe and the USA, are closely associated with significant mortalities of the Pacific oyster, Crassostrea gigas, especially during its early stages of life. In summer 2006, we monitored mortality by strict daily verification of three full-sib families of oysters reared under common conditions. We quantified OsHV-1 using real-time PCR in dead and living individuals during and after a mortality event. Mortality events were severe and brief, but significantly different between tested families (cumulative mortality ranging from 1.2 to 49%). Real-time PCR assays revealed different viral DNA loads in dead individuals from different families (P &lt; 0.001). Moreover, the mean level of infection...
Tipo: Text
Palavras-chave: Viral DNA quantification; Real time PCR; OsHV 1; Mortality; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6265.pdf

Individual growth variation and its relationship with survival in juvenile Pacific oysters, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, B; Mccombie, Helen; Ernande, Bruno; Morand, B; Heurtebise, Serge; Gerard, Andre.
In order to study individual growth variability and its relationship with survival in juvenile Crassostrea gigas, parental oysters were sampled at four sites located along the French Atlantic coast and bred under controlled hatchery conditions. Four groups of larvae were obtained by crossing five males and five females from each of the four sites, and a fifth group by crossing these 20 males and 20 females together in a pool. Larvae were reared under conditions allowing the maintenance of a maximum variability of size and gave five experimental groups. Oysters were individually monitored for growth and survival from 3 to 10 months after fertilization. The individual growth performances were relatively stable over time and no noticeable compensation for...
Tipo: Text
Palavras-chave: Survival; Oysters; Growth; Crassostrea gigas; Aquaculture.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-467.pdf

Modulations of the interactions between pacific cupped oyster Crassostrea gigas and Vibrio according to bacterial virulence and to genetic and physiological status of the host : Methodology and first results
Provedor de dados: 5
Autores: De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis.
Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and...
Tipo: Text
Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf

Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites
Provedor de dados: 5
Autores: Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois.
High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes).   Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered...
Tipo: Text
Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf

Overwiew on selective breeding and genetic improvement in bivalve shellfish
Provedor de dados: 5
Autores: Boudry, Pierre.
Dr. Boudry focussed his talk on oysters, which are the most important bivalve species produced world wide (more than 4 million tons). One of the main characteristic of bivalve aquaculture is that most of the juveniles ("seed or "spat") are collected from natural recruitment (e.g. 100% of the European mussel production). Hatchery propagation is used in the case of introduced species (e.g. Crassostrea gigas in USA)  or limited natural recruitment.    The most significant genetic improvement for the production of Pacific oyster (C. gigas) to date has been obtained through the breeding of triploids, especially since the development of tetraploids. Triploidy lead to highly reduced gametogenesis and, as a result better growth and survival.  Many quantitative...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oyster; Genetic variability; Bivalve shellfish; Selective breeding; Genetic.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3479.pdf

Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L. (2003)
Provedor de dados: 5
Autores: Lapegue, Sylvie; Taris, Nicolas; Boudry, Pierre; Langlade, Aime; Bonhomme, Francois.
Le milieu marin est un milieu difficile à appréhender car composé d'un ensemble d'organismes et d'écosystèmes caractérisés par des conditions de fonctionnement tout à fait originales et souvent difficiles d'accès. Ces spécificités peuvent être attribuées à la structure spatiale du milieu marin, qui à bien des égards, diffère du milieu terrestre. En effet si les notions de barrières géographiques sont une réalité concrète pour les organismes terrestres, elles restent rarement identifiées en milieu marin (PALUMBI 1994). Dans un tel environnement, les organismes présentent des cycles de vie souvent mal connus, notamment ceux qui associent phase benthique et phase planctonique. L'étude de la structure génétique des populations, dans un espace aussi ouvert aux...
Tipo: Text
Palavras-chave: Diversité génétique; Génétique; Recrutement; Génotypage; Variabilité génétique; Huître plate; Ostrea edulis.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00044/15478/12857.pdf

Fitness landscapes support the dominance theory of post-zygotic isolation in the mussels Mytilus edulis and M. galloprovincialis
Provedor de dados: 5
Autores: Bierne, Nicolas; Bonhomme, Francois; Boudry, Pierre; Szulkin, M; David, Pierre.
We studied the genetic basis of post-zygotic isolation in the marine mussels Mytilus edulis and Mytilus galloprovincialis. Evidence was obtained for a high number of recessive Dobzhansky-Muller substitutions in the genome of these two mussel taxa. We analysed the segregation of unlinked diagnostic markers in the progeny of two backcrosses and an F-2 cross, 36 h and 200 days after fertilization. Directional selection favouring M. galloprovincialis genotypes was observed in both kinds of cross. In the F-2, epistatic interactions between each pair of chromosome fragments mapped by the markers were identified in addition. Our results imply that homozygous-homozygous interactions are required for breakdown of coadaptation, in accordance with the dominance...
Tipo: Text
Palavras-chave: Mytilus; Dobzhansky Muller incopatibilities; Post zygotic isolation; Negative epistasis; Hybrid breakdown; Hybrid zone.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1350.pdf

Genetic basis of growth, survival and their plasticity in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis.
Introduction : Growth and survival are the life-history traits of the most interest for oyster production since they determine biomass output (growth x survival). This study aimed to investigate whether there is genetic basis for growth, survival and their plasticity to know if a selection program is conceivable for these traits. Material and Methods : Nested half-sibs mating design was used to produce families of oysters: 5 males were each mated to three females producing 5 half-sibs families, each containing 3 full-sib families. At 15 months of age (May 1999), each full-sibs family was randomly split into five groups which were given different treatments. Two groups were placed in a constant environment: one with low food resources and another with high...
Tipo: Text
Palavras-chave: Genotyic; Plasticity; Survival; Growth; Genetic; Crassostrea gigas; Pacific oyster.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3498.pdf

Mise au point d'outils de gestion des gamètes mâles d'huîtres creuses Crassostrea gigas diploïdes et tétraploïdes à des fins de conservation et de diffusion
Provedor de dados: 5
Autores: Brizard, Raphael; Boudry, Pierre; Haffray, Patrick; Labbe, C.; Suquet, Marc; Robert, Rene; Masse, G.; Pepin, Jean-francois; Saulnier, Denis.
In order to improve oyster culture, many research programs try and propose new methods aiming at improving genetically the product performances and quality (selection, polyploidisation). As for terrestrial animal production, the continuity of the different works as well as the spreading of the genetical progress would be made easier by the development of conservation techniques of the selected genotypes. Cryopreservation, which is often used in selection programs of terrestrial animals or fish farming species, is not always available for bivalves. This technique would enable the conservation of the gametes of individuals selected for their high growth potential, their resistance to diseases and/or their quality for a smaller cost and less risks than the...
Tipo: Text
Palavras-chave: Tetraploïde; Diploïde; Concervation; Spermatozoides; Cryoconservation; Génétique; Crassostrea gigas; Huîtres.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3339.pdf

Etude du polymorphisme des gènes de l'amylase chez Crassostrea gigas : relation avec les paramètres physiologiques de l'assimilation et croissance
Provedor de dados: 5
Autores: Degremont, Lionel; Moal, Jeanne; Daniel, Jean-yves; Boudry, Pierre; Van Vormhoudt, A.; Sellos, D.; Samain, Jean-francois.
In the scope of the European program GENEPHYS, which aims to establish the relationship between the genetic base and the physiological characters in relation to the growth in Crassostrea gigas, it has been demonstrated that the most explanatory factors for the growth variability are assimilation performance and aneuploidy. A model linking the assimilation performances and the digestive equipment has been set up and more than 70% of the variability can be explained by two digestive enzymes: laminarinase and amylase. A number of laboratories have decided to focus on the latter.
Tipo: Text
Palavras-chave: Croissance; Génétique; Polymorphisme; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3269.pdf

Is fertility of hybrids enough to conclude that the two oysters Crassostrea gigas and Crassostrea angulata are the same species?
Provedor de dados: 5
Autores: Huvet, Arnaud; Gerard, Andre; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Boudry, Pierre.
The distinction of the two cupped oysters Crassostrea gigas (Thunberg, 1793) and Crassostrea angulata (Lamark, 1819) into two species was chiefly due to their differing geographical distributions, C. gigas being present in Asia and C. angulata in Europe. Today it is commonly accepted that C. angulata and C. gigas are a single species according to morphological, genetic and F1 hybridization data. However, the demonstration of the fertility of their hybrids and the absence of any reproductive isolation remained to be investigated. Consequently, we studied the fertility of hybrids and sperm competition by performing three different experiments and producing G1 and G2 hybrid progenies between wild populations of C. angulata and C. gigas. Progenies showed very...
Tipo: Text
Palavras-chave: Crassostrea angulata; Crassostrea gigas; Developmental yields of larvae; Fertility; Controlled crosses; Hybridization.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-1395.pdf

Oocyte activation in Mytilus edulis by Crassostrea gigas spermatozoa
Provedor de dados: 5
Autores: Mccombie, Helen; Cornette, Florence; Beaumont, Andy; Boudry, Pierre.
The development of hatchery production techniques for blue mussels is receiving increased research attention in Europe as it could offer an alternative source of spat for the industry ('Blue Seed' Project, Kamermans ICSR 2007). Among the necessary techniques are those of controlled spawning and fertilization, and techniques of triploidisation that can offer increased seasonal availability, yield and/or decreased growing time in the subsequent production stages. In several experiments, Mytilus edulis oocytes, obtained by thermally induced spawning, were fertilized with Mytilus edulis spermatozoa obtained in the same way or by stripping.  Parallel treatments examined the effect of mixing Mytilus edulis oocytes with spermatozoa stripped from the Pacific...
Tipo: Text
Palavras-chave: Stripping; Spermatozoa; Crassostrea gigas; Mytilus edulis; Ooctye.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3460.pdf

Forty questions of importance to the policy and practice of native oyster reef restoration in Europe
Provedor de dados: 5
Autores: Zu Ermgassen, Philine S. E.; Bonačić, Kruno; Boudry, Pierre; Bromley, Cass A.; Cameron, Tom C.; Colsoul, Bérenger; Coolen, Joop W. P.; Frankić, Anamarija; Hancock, Boze; Have, Tom M.; Holbrook, Zoë; Kamermans, Pauline; Laugen, Ane T.; Nevejan, Nancy; Pogoda, Bernadette; Pouvreau, Stephane; Preston, Joanne; Ranger, Christopher J.; Sanderson, William G.; Sas, Hein; Strand, Åsa; Sutherland, William J.
Oyster reefs are among the most threatened marine habitats globally. In Europe, oyster reefs have been extirpated from most locations within their historical range. Active restoration of the native oyster (Ostrea edulis) in Europe has grown substantially in recent years. In sharing experiences between oyster restoration projects in Europe at the Native Oyster Restoration Alliance conference, NORA2, in Edinburgh in May 2019, it became apparent that a number of similar barriers are experienced. This study identified the top 40 questions, which, if answered, would have the greatest influence on the policy and practice of oyster restoration in Europe. Initially 71 people were consulted across 28 institutions and 11 European countries to generate 194 questions....
Tipo: Text
Palavras-chave: Alien species; Estuary; Fishing; Invertebrates; Pollution; Restoration; Subtidal.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00658/77004/78261.pdf

Comparative karyological study of cupped oyster species
Provedor de dados: 5
Autores: Leitao, Alexandra; Boudry, Pierre; Labat, Jean-philippe; Thiriot-quievreux, Catherine.
Chromosomes of six cupped oyster species were studied using karyometric analysis alter conventional Giemsa staining, and silver staining, Karyotypes of Crassotrea angulata (nine metacentric and one submetacentric chromosome pairs), C. sikamea (nine metacentric and one submetacentric chromosome pairs), C. virginica (eight metacentric and two submetacentric chromosome pairs), C, ariakensis (eight metacentric and two submetacentric chromosome pairs), C, gasar (six metacentric and four submetacentric chromosome pairs), and Saccostrea commercialis (eight metacentric and two submetacentric chromosome pairs) are distinguishable by the number and position of the submetacentric chromosome pair and by the location of nucleolus organizer regions, Comparative...
Tipo: Text
Palavras-chave: Bivalvia; Cytotaxonomy; Karyotype; Chromosome; Cupped oyster.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-2737.pdf

Mapping QTL for resistance to summer mortality in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Heurtebise, Serge; De Koning, D.; Haley, Chris; Boudry, Pierre; Lapegue, Sylvie.
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). Such selective breeding would strongly benefit from the identification of markers associated to the resistance to allow the development of markers assisted selection. Recently, a set of a new kinds of markers (SNPs) have been developed in the Pacific oyster. These markers were identified and characterized in several genes involved in the immune response and energetic pathways (Sauvage, 2007). In this context, the mapping of SNPs, of 16 new EST-SSR based markers and of...
Tipo: Text
Palavras-chave: Mortality; Genetic; Crassostrea gigas; Pacific oyster; Mapping QTL.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3504.pdf

Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Normand, Julien; Li, Ronghua; Quillien, Virgile; Nicolas, Jean-louis; Boudry, Pierre; Pernet, Fabrice; Huvet, Arnaud.
Pacific oyster Crassostrea gigas suffers from chronic or sporadic mortality outbreaks worldwide, resulting from infectious diseases and/or physiological disorders triggered by environmental factors. Since 2008, ostreid herpesvirus OsHV-1 μVar has been identified as the main agent responsible for mass mortality of juvenile oysters in Europe. Previous studies of genome-wide expression profiling have provided candidate genes that potentially contribute to genetically-based resistance to summer mortality. To assess their value in determining resistance to the juvenile mass mortality that has occurred in France since 2008, we analyzed the expression of 17 candidate genes in an experimental infection by OsHV-1 μVar, and in an in vivo field experiment. Individual...
Tipo: Text
Palavras-chave: Molluscs; Gene expression profiling; Inhibitor of kappa B; Summer mortality; Ostreid herpesvirus.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00177/28823/27325.pdf

Interspecific hybridization in oysters: Restriction enzyme digestion chromosome banding confirms Crassostrea angulata x Crassostrea gigas F1 hybrids
Provedor de dados: 5
Autores: Leitao, A; Chaves, R; Santos, S; Guedes Pinto, H; Boudry, Pierre.
The taxonomic status of the two commercially important cupped oysters, Crassostrea angulata, the Portuguese oyster (Lamarck, 1819) and Crassostrea gigas, the Japanese oyster (Thunberg, 1793) has long been in question. The recent observation of the hybridization between C. gigas and C. angulata and the production of fertile F1s led us to search for cytogenetic evidence of both parental genomes in the interspecific hybrids. The cytogenetic characterization of the hybrids was performed by the use of restriction endonuclease treatments. This technique has recently shown the potential for individual chromosome identification by banding in oysters. Chromosomes of C. gigas, C. angulata and their hybrids were treated with two different restriction enzymes (ApaI...
Tipo: Text
Palavras-chave: Interspecific hybrids; In situ restriction enzyme banding; Crassostrea gigas; C. angulata; Chromosome banding.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-4959.pdf

Bonamia ostreae-induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf

Phenotypic and genetic consequences of size selection at the larval stage in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Taris, Nicolas; Ernande, Bruno; Mccombie, Helen; Boudry, Pierre.
The life histories of oysters in the genus Crassostrea, like those of most marine bivalves, are typified by high fecundity and low survival in nature. Rearing conditions in hatcheries however ensure optimized density, diet, and temperature. Hatcheries are becoming increasingly important for the production of juveniles in aquaculture, and their culture practices often include culling of slow growing larvae to reduce and synchronize the time taken to reach settlement. Because previous studies have found substantial genetic variation for early life developmental traits in Crassostrea gigas, these culling practices are likely to cause highly different selective pressures in hatcheries from those in the natural environment. We studied the phenotypic and genetic...
Tipo: Text
Palavras-chave: Oysters; Larvae; Hatchery; Genetic diversity; Culling; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1677.pdf

Single Nucleotide polymorphisms and their relationship to codon usage bias in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Bierne, N; Lapegue, Sylvie; Boudry, Pierre.
DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other...
Tipo: Text
Palavras-chave: Crassostrea gigas; Codon bias; Genetic diversity; SNP.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3417.pdf

Variabilité génétique de la réponse aux organismes pathogènes : un outil pour améliorer la santé des mollusques et poissons d'élevage
Provedor de dados: 5
Autores: Quillet, Edwige; Boudry, Pierre; Lapegue, Sylvie.
Controlling health of livestock has become of considerable importance to the aquaculture industry. Selective breeding of animals with increased resistance to pathogens is likely to complete efficiently the use of drugs, vaccines or prophylactic approaches that may be difficult to implement. A review of the scientific literature shows that the prospect of selecting for increased resistance is quite hopeful in both molluscs and fish. Yet, selecting for disease resistance is hampered by the difficulty to measure the resistance of individuals. Prospects for the development of «markers» that would predict the genetic merit of individuals without pathogen challenge are presented. The breeding schemes that may be implemented for fish and molluscs in the French...
Tipo: Text
Palavras-chave: Marqueurs phénotypiques; Marqueurs génétiques; Poissons; Mollusques; Agents pathogènes; Variabilité génétique.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2979.pdf

Etude de la connectivité potentielle en rade de Brest : application au cas de l'huître plate, Ostrea edulis
Provedor de dados: 5
Autores: Bernard, Ismael; Duchene, Julie; Hily, Christian; Lazure, Pascal; Pouvreau, Stephane; Boudry, Pierre.
En complément du rapport final (Anonyme (2014). PERLE. Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis. Rapport final de l'ensemble du projet 2011-2014. http://archimer.ifremer.fr/doc/00248/35932/), ce rapport présente l'étude de la phase de dispersion des larves en rade de Brest et analyse le lien entre la quantité de larves émises et la quantité de larves captées. Ce lien, qui possède une forte composante spatiale, est appelé connectivité. Il est sous l’influence de la dispersion et de la survie des larves mais seule la dispersion larvaire, avec différents forçages biophysiques du milieu, a été considérée ici. Les effets de la localisation des zones d’émission et de captage, du comportement des larves, du vent ou de la durée...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00251/36233/34793.pdf

Relationships between growth, survival, physiology and behaviour — A multi-criteria approach to Haliotis tuberculata phenotypic traits
Provedor de dados: 5
Autores: Lachambre, Sebastien; Huchette, Sylvain; Day, Rob; Boudry, Pierre; Rio-cabello, Antoine; Fustec, Timothee; Roussel, Sabine.
Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored...
Tipo: Text
Palavras-chave: Haliotis tuberculata; Growth; Behaviour; Physiology; Multivariate analysis; Phenotyping.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00333/44434/44101.pdf

Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
The most significant genetic improvement for production of Pacific oyster (Crassastrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have slowed the development of selective breeding programs. Recently, in the USA, Australia and New Zealand, family-based selective breeding programs have been initiated to improve growth and yield. In Europe, where both natural and...
Tipo: Text
Palavras-chave: Microsatellites; Quantitative genetic; Genetic variability; Crassostrea gigas; Oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2861.pdf

Approche comparative de la variabilité et de la structuration génétique chez deux espèces d'huîtres cultivées (Crassostrea gigas et Ostrea edulis) présentant des stratégies reproductives et des histoires contrastées
Provedor de dados: 5
Autores: Boudry, Pierre; Launey, Sophie; Huvet, Arnaud.
The two common oyster species cultivated in Europe are the flat oyster Ostrea edulis and the cupped oyster Crassostrea gigas. These species have two really different reproduction strategies (oviparity versus larviparity) and histories (natural distribution versus introductions linked to oyster culture). We used microsatellite markers to compare the observed variabilities Europe wide. O. edulis presents a lower rate of alleles per locus than C. gigas. The observed structuration levels (global Fst and by pairs values) are similar for both species, but the spatial organisation of the diversity is different. In O. edulis, appears a differentiation based on the distance (concordance of the results with those obtained with the allozymes) whereas in C. gigas,...
Tipo: Text
Palavras-chave: Différenciation génétique; Variabilité; Génétique; Crassostrea gigas; Ostrea edulis; Huîtres.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/acte-3455.pdf

Genetical improvement of marine fish and shellfish : a french perspective
Provedor de dados: 5
Autores: Boudry, Pierre; Chatain, Beatrice; Naciri-graven, Yamama; Lemaire, Christophe; Gerard, Andre.
In France, marine fish and shellfish farming is based mainly on the breeding of wild species, whose natural populations are traditionally exploited. Research programs are conducted by IFREMER, in co-operation with INRA and Universities. French research programs for the genetical improvement of marine fish and shellfish concern primarily seabass (Dicentrarchus labrax) and oysters (Ostrea edulis and Crassostrea gigas). The following programs are developed:  •The knowledge of wild and farmed populations. The most recent prodjects involve the highly variable microsatellite markers. Seven markers are presently available for seabass and the work is now initiated on turbot (Scophthalmus maximus). In molluscs, microsatellite markers have been developed for the...
Tipo: Text
Palavras-chave: Selection; Triploid; Shellfish; Fish; Genetic.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/1996/acte-3206.pdf

Summer mortality of selected juvenile pacific oyster Crassostrea gigas under laboratory conditions and in comparison with field performance
Provedor de dados: 5
Autores: Degremont, Lionel; Soletchnik, Patrick; Boudry, Pierre.
This study reports mortality under laboratory conditions in unselected controls and two lines of juvenile Pacific oysters Crassostrea gigas, previously selected for their high or low survival in the field during the summer period. Oysters were also deployed in field conditions and mortality between both conditions was then compared. In the laboratory, mortality was observed in all experiments and it always lasted for a week indicating that mortality under laboratory conditions was a short-term event. It was also shown that mortality could be repeated for a batch in several experiments using oysters which never experienced any abnormal mortality. This approach could facilitate further studies to investigate the causes of mortality by allowing repeated...
Tipo: Text
Palavras-chave: Summer mortality; Crassostrea gigas; Laboratory; Field; Oyster; Juvenile.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12432/9228.pdf

The molecular phylogeny of oysters based on a satellite DNA related to transposons
Provedor de dados: 5
Autores: Lopez Flores, Inmaculada; De La Herran, R; Garrido Ramos, M; Boudry, Pierre; Ruiz Rejon, C; Ruiz Rejon, M.
We have analysed a centromeric satellite DNA family that is conserved in several commercial and non-commercial oyster species (Ostrea edulis, O. stentina, Crassostrea angulata, C gigas, C gasar, C ariakensis, C virginica and C sikamea). This satellite DNA family is composed of AT-rich repeat sequences of 166+/-2 bp and presents a 9-bp motif similar to the mammalian CENP-B box. The homology of oyster HindIII satellite DNA with satellite DNAs from other bivalves and its relation to a part of a mobile element suggest the existence of an ancient transposable element as a generating unit of satellite DNA in bivalve molluscs. Taking advantage of its degree of conservation in oyster species, we have used this element as a taxonomic marker. This marker clearly...
Tipo: Text
Palavras-chave: Satellite DNA origin; Genetic identification; CENP B box; Crassostrea; Ostrea.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-761.pdf

Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality
Provedor de dados: 5
Autores: Fleury, Elodie; Moal, Jeanne; Boulo, Viviane; Daniel, Jean-yves; Mazurais, David; Henaut, Alain; Corporeau, Charlotte; Boudry, Pierre; Favrel, Pascal; Huvet, Arnaud.
Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially...
Tipo: Text
Palavras-chave: Mollusca; Bivalvia; Gene expression; CDNA microarrays; Reproduction; Oxidative stress; Energy metabolism.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00005/11590/8258.pdf

Is the Portuguese oyster Crassostrea angulata in Southern Europe endangered by the expansion of the Pacific oyster C. gigas?
Provedor de dados: 5
Autores: Batista, Frederico; Boudry, Pierre; Lapegue, Sylvie; Heurtebise, Serge; Monteiro, C. C.
The Portuguese oyster, Crassostrea angulata, was introduced from Portugal to the French Atlantic Coast in the 1860s. C. angulata quickly settled and expanded and leaded to the development of a new aquacultural industry in France. In the late 1960s, mortality associated with the detection of an iridovirus, led to the wipe out of C. angulata from French Atlantic waters and to the massive introduction of C. gigas to sustain production. In Southern Europe, similar symptoms were also observed in natural stocks of C. angulata from Sado River (Portugal) and from the area of Cadiz (Spain). Nowadays, only very few «pure» populations of C. angulata remain in southern Europe. These populations are potentially endangered by the current expansion of C. gigas...
Tipo: Text
Palavras-chave: Selection; Hybridization; Settlement pattern; Population genetic; Genetic; Oysters; Crassostrea angulata; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3310.pdf

Impact of atrazine on somatic aneuploidy in cupped oysters, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Chaves, R.; Guedes Pinto, H; Boudry, Pierre; Lapegue, Sylvie.
In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species
Tipo: Text
Palavras-chave: Genetic; Aneuploidy; Toxicology; Impact; Atrazine; Crassostrea gigas; Pacific oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3489.pdf

Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - Third Progress Report 1st January-31st December 1998 - Contract N°FAIR 95-421 Commission of the European Communities
Provedor de dados: 5
Autores: Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Buestel, Dominique; Samain, Jean-francois; Hawkins, A. J. S.; Wilins, N.; Zouros, Eleftherios; Bonhomme, Francois; Thiriot Quievreux, Catherine.
The EU project "GENEPHYS", initiated by the IFREMER laboratory GAP-La Tremblade (Génétique Aquaculture et Pathologie) and CREMA-l'Houmeau (Centre de Recherche en Ecologie Marine et Aquaculture), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main bjectives are : 1. To express the maximum variability of growth, both at the larval stage and at the adult stage, in a oyster population originating from parents of various origins. 2. To check the persistence over the time, of growth performance and related physiological functions. 3. To explain...
Tipo: Text
Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Growth; Absorption efficiency; Oysters; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00050/16151/13634.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Contrat Région Poitou-Charentes 1994-1998 - 1ère partie : rapport année 1994
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Launey, Sophie; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous Ies membres du Réseau Génétique Mollusques (REGEMO) a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses. Le contrat de plan Etat-Région a été signé en...
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Saccostrea; Génétique population.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00044/15517/12904.pdf

A first linkage map for the European flat oyster, Ostrea edulis towards the identification of Bonamia resistance QTLs
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, a genetic map was therefore established as a basis for Quantitative Trait Loci (QTLs) analysis. The reference family was an F2 family coming from a first bi-parental cross between a wild oyster and an individual from the fifth generation inbred line of the Bonamia tolerance selection program. This inbred line had shown no mortality when reared in...
Tipo: Text
Palavras-chave: Parasite; Bonamia ostreae; Bonamiosis; QTL; Genetic; Ostrea edulis; Flat oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3459.pdf

"First class oysters" : progress and constgraints in genetic improvement of the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Boudry, Pierre.
The most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) up until now has been obtained through the production of triploids, particularly since the development of tetraploids in the mid 90s. Alternatively, quantitative genetics studies suggest that significant gains could be obtained in traits of aquacultural interest. However, the limited extent of hatchery propagation (compared with natural recruitment) in some countries and/or technical difficulties and biological characteristics of this species have retarded the development of selective breeding programs for C. gigas. Individual selection can however be performed easily in such a highly fecund species, though it often leads to small effective population sizes...
Tipo: Text
Palavras-chave: Selective breeding; Genetic; Crassostrea gigas; Pacific oysters.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3462.pdf

Défi MOREST - Mortalités estivales de l'huître creuse Crassostrea gigas - Caracterisation des facteurs de risques associés au mortalités estivales - Synthèse du thème IVA - Etude des bases génétiques de la mortalité estivale du naissain et des possibilités d'amélioration de la survie par sélection. (La Rochelle 14- 15 mars 2006)
Provedor de dados: 5
Autores: Boudry, Pierre.
Malgré l'importance économique de la production de I'huître creuse Crassostrea gigas en France et dans le monde, cette espèce n'a encore que peu bénéficié des améliorations observées chez la quasi-totalité des espèces d'intérêt agricole et un nombre croissant d'espèces d'intérêt aquacole. Le principal apport de la génétique à l'amélioration de la production de cette espèce consiste en la production d'individus triploïdes (pour revue voir Nell, 2002). Chez les huîtres, les principaux objectifs des programmes de sélection ont été classés par Mahon (1983) en quatre groupes principaux par caractères d'intérêts : (a) caractères liés à la ponte et au développement larvaire ; (b) amélioration de la survie et de la croissance de la fixation à la taille commerciale...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00304/41534/40742.pdf

Acclimatation de nouvelles espèces d'huîtres creuse du genre Crassostrea: hybridation et conservatoire de souches - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Synthèse finale
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Huvet, Arnaud; Chollet, Bruno; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Actions menées de 1994 à 1998 au sein du conservatoire de souches : 1. Résultats des importations, acclimatation et hybridation. a) Rappel des conditions d'introduction de nouvelles souches. L'importation de toute espèce est conditionnée par les résultats d'une enquête préliminaire dont l'objet est de rechercher les exigences écologiques de l'espèce et la situation épidémiologique dans son milieu d'origine. Le travail du LGP et de ses partenaires a été partagé en différentes actions: 1. Recherche de contacts dans divers pays étrangers, en vue de l'importation d'huîtres présentant des caractéristiques intéressantes. 2. Importation : L'importation se fait selon les normes du CIEM (Conseil International pour l'Exploration de la Mer) : contrôles pathologiques...
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Marqueurs moléculaire; Marqueurs populations; Crassostrea.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00044/15520/12907.pdf

Genetic structure at different spatial scales in the pearl oyster (Pinctada margaritifera cumingii) in French Polynesian lagoons: beware of sampling strategy and genetic patchiness
Provedor de dados: 5
Autores: Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre.
In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat...
Tipo: Text
Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf

Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters
Provedor de dados: 5
Autores: De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume.
Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Rapport année 1996
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Collet, Bertrand; Cornette, Florence; Heurtebise, Serge; Ledu, Christophe; Chollet, Bruno; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1996 étaient les suivants: Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant Limitée).
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Sélection génétique; Saccostrea commercialis.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15516/12903.pdf

Bonamia-like parasite found in the Suminoe oyster Crassostea rivularis reared in France
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea. Thus,...
Tipo: Text
Palavras-chave: Bonamia like parasite; Bonamiosis; Simunoe oyster; Crassostrea rivularis.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-2904.pdf

Environmental anomalies, energetic reserves and fatty acid modifications in oysters coincide with an exceptional mortality event
Provedor de dados: 5
Autores: Pernet, Fabrice; Barret, Jean; Marty, Claire; Moal, Jeanne; Le Gall, Patrik; Boudry, Pierre.
Mortality of oysters Crassostrea gigas occurred along the coasts of France during summer 2008, causing a serious crisis in French oyster production. During spring to summer 2008, 5 groups of 1-yr old oysters of different origins and ploidy levels deployed in the Thau lagoon (France) were sampled for biometrical and biochemical analyses; environmental parameters were monitored in the same area. Mortality occurred in 2 episodes: 13 May to 10 June and 24 June to 9 July. Wild-collected oysters showed mortality sooner than other groups but total overall mortality was 85% in all groups. Mortality occurred when seawater temperature was &gt;19 degrees C during the reproductive season when energetic reserves of oysters were at their lowest. Energy reserve levels...
Tipo: Text
Palavras-chave: Bivalves; Energetic reserve; Environment; Fatty acid; Summer mortality; Trophic signatures; Temperature.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11388/7964.pdf

Niveau de ploïdie des huîtres des bassins de captage de Marennes-Oléron et Arcachon
Provedor de dados: 5
Autores: Cornette, Florence; Grouhel, Stephanie; Sharbel, Tim; Boudry, Pierre; Goulletquer, Philippe; Lapegue, Sylvie.
La mise en place du réseau « biovigilance » résulte des recommandations formulées dans le cadre de l’expertise indépendante demandée par le Comité Scientifique du Ministère de l’Agriculture et de la Pêche concernant « l’effet d’un flux éventuel d’huîtres tétraploïdes dans les zones conchylicoles ». Il avait été alors préconisé de réaliser « une biovigilance légère, avec mesure régulière du taux d’huîtres tétraploïdes dans les bassins conchylicoles ». Le sous-projet « Réseau biovigilance » s’inscrit dans le projet Ifremer « Typologie et surveillance des systèmes de productions aquacoles » du programme de recherches «Durabilité des systèmes de production » du thème « Surveillance et optimisation des productions aquacoles ». Ce réseau a pour objectif d’être...
Tipo: Text
Palavras-chave: Reproduction; Captage; Arcachon; Marennes Oléron; Génétique; Crassostrea gigas; Ploïdie; Huîtres.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00043/15445/12815.pdf

Variance of reproductive success in the European flat oyster Ostrea edulis assessed by microsatellite-based parentage analyses in a natural population and in hatchery
Provedor de dados: 5
Autores: Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie.
he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,...
Tipo: Text
Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf

Impact génétique de la production intensive d'huîtres en écloserie : processus de dérive et de sélection liés aux pratiques d'élevage larvaire
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Oysters, like many other marine bivalves, are characterized by high fertility and low survival rates during the precocious stages. Hatchery reproduction, realised in very different conditions than in their natural habitat (density, food, temperature, etc.), tends to optimize the production of larvae and juveniles. As a consequence, slow growing larvae are systematically culled. Previous results suggest the existence of a genetic variability linked to precocious development. Our approach consists in studying the evolutionary process (genetic drift and selection) linked with hatchery reproduction. Thanks to microsatellite markers enabling us to analyse the relatedness in "mixed" cultivated families, we studied the variability of breeding success in...
Tipo: Text
Palavras-chave: Crassostrea gigas; Huîtres; Marqueurs microsatellites; Reproduction; Sélection; Dérive génétique; Variabilité génétique; Génétique.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3481.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat de Plan Région Poitou-Charentes 2000-2006. Convention 2000
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00043/15474/12853.pdf

Aneuploidy and its relationship with growth in different populations of the Pacific oyster (Crassostrea gigas, Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Leitao, Alexandra; Mccombie, Helen; Thiriot-quievreux, Catherine.
Cytogenetic abnormalities arising both in mitosis and meiosis are known to be common in bivalves. Here we review result obtained from the observations of somatic aneuploidy in different populations of the commercially important Pacific oyster, Crassostrea gigas. The oysters studied were either (1) produced in IFREMER hatcheries between 1986 and 1999, (some of these were part of the EU funded programme GENEPHYS); or (2) collected from wild populations along the French Atlantic coast. Chromosome numbers were scored from 30 mitotic metaphases in gill tissue per  individual studied. Aneuploid cells (2n - X) were observed in all the 13 populations studied. A highly significant negative correlation was observed between the level of aneuploidy and growth, i.e....
Tipo: Text
Palavras-chave: Growth; Genetic; Aneuploidy; Crassostrea gigas; Pacific oysters.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3446.pdf

Evidence of differential chromosome loss in aneuploid karyotypes of the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Leitao, Alexandra; Boudry, Pierre; Thiriot Quievreux, Catherine.
The G-banding technique was performed on aneuploid karyotypes from gill tissue of the Pacific oyster, Crassostrea gigas, to assess whether chromosome losses could be explained by differential chromosomal susceptibility and to clarify the negative correlation between aneuploidy and growth rate previously reported in different populations of this oyster. The study of 95 G-banded aneuploid karyotypes showed that only 4 of the 10 chromosome pairs (viz. 1, 5, 9, and 10) of C. gigas were affected by the loss of one homologous chromosome. Pairs 1, 9, and 10, which were lost in 56, 33, and 44% of cases, respectively, may be considered to be differentially affected. Hypotheses on this differential chromosomal susceptibility are discussed.
Tipo: Text
Palavras-chave: Oyster; Karyotype; G banding; Growth; Aneuploidy; Huître; Caryotype; Bandes G; Croissance; Aneuploïdie.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-742.pdf

Trans-Atlantic distribution of a mangrove oyster species revealed by 16S mtDNA and karyological analyses
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre.
Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been...
Tipo: Text
Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf

Genetic basis of gametogenesis in diploid and triploid Pacific oysters, Crassostrea gigas
Provedor de dados: 5
Autores: Normand, Julien; Ernande, Bruno; Boudry, Pierre.
Today, triploidy is the most common method used to genetically improve marine bivalve aquaculture production. In the Pacific oyster Crassostrea gigas, triploidy affects many traits. The most proeminent one is reproductive effort, which is greatly reduced compared to diploids. The combined effects of physiological trade-offs between survival, growth and reproduction together with the reduction of reproductive effort in triploids explains the global enhancement of yield observed. However, the sterility of triploid oysters is only partial and some triploid individuals can show normal gametogenesis (i.e. similar to diploids), especially under warm conditions. In diploid oysters, the heritability of reproductive effort has been shown to be relatively low, but a...
Tipo: Text
Palavras-chave: Parentage analysis; Triploidy; Oyster; Quantitative genetics; Gametogenesis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4525.pdf

Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Leitao, Alexandra; Chaves, Raquel; Guedes Pinto, Henrique; Boudry, Pierre; Lapegue, Sylvie.
Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Restriction enzyme digestion chromosome banding; Atrazine; Aneuploidy; Crassostrea gigas; Huître creuse; Marquage chromosomique par digestion d'enzymes de restriction; Atrazine; Aneuploïdie.
Ano: 2005
URL: https://archimer.ifremer.fr/doc/2005/publication-654.pdf

Mortalités estivales et croissance du naissain de l'huître creuse Crassostrea gigas : étude de familles sélectionnées
Provedor de dados: 5
Autores: Degremont, Lionel; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Soletchnik, Patrick; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois.
In the scope of the Morest program, 3 series of bi-parental families of cupped oyster consisting of full and half-sib have been created in 2001, and then studied on a regular basis on several sites over the summer period. In October, major survival differences had been demonstrated between the 3 families. The average survival rate for the half-sib families monitored on the three sites varies from 17 to 97 %. Some of them showed high mortality for the three sites, indicating a strong genetic basis (h2 = 0.81 - 0.29) to the variations observed regarding survival. To a lesser extent, growth differences between the studied half-sib families indicate a weaker heritability for this character. ....
Tipo: Text
Palavras-chave: Physiologie; Génétique; Naissain; Sélection; Croissance; Survie; Huîtres; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3490.pdf

QTL for resistance to summer mortality and OsHV-1 load in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Sauvage, Christopher; Boudry, Pierre; De Koning, D. -j.; Haley, C. S.; Heurtebise, Serge; Lapegue, Sylvie.
P&gt;Summer mortality is a phenomenon severely affecting the aquaculture production of the Pacific oyster (Crassostrea gigas). Although its causal factors are complex, resistance to mortality has been described as a highly heritable trait, and several pathogens including the virus Ostreid Herpes virus type 1 (OsHV-1) have been associated with this phenomenon. A QTL analysis for survival of summer mortality and OsHV-1 load, estimated using real-time PCR, was performed using five F-2 full-sib families resulting from a divergent selection experiment for resistance to summer mortality. A consensus linkage map was built using 29 SNPs and 51 microsatellite markers. Five significant QTL were identified and assigned to linkage groups V, VI, VII and IX. Analysis of...
Tipo: Text
Palavras-chave: Crassostrea gigas; Herpes virus; Pacific oyster; QTL mapping; Summer mortality.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12241/9063.pdf

NPY signalling in the gonad of the oyster Crassostrea gigas : involvement in summer mortality?
Provedor de dados: 5
Autores: Corporeau, Charlotte; Fleury, Elodie; Moal, Jeanne; Boudry, Pierre; Favrel, Pascal; Huvet, Arnaud.
In summer, oysters suffer mortality that decimates this year about 60% of the population in France. We previously demonstrated a genetic basis for summer survival thatallowed us to produce resistant (R) and susceptible (S) oyster familiesby divergent selection (Samainet al., 2007). At the physiological level, R families can survive summer mortality because they are reproductively less active than S families (Fleury et al., 2008).In order to identify the molecular basis of the summer survival,a transcriptomicapproach on R and S families was performed using microarray analysis and 34 differentially-expressed genes were identified between R and S in the gonad (Fleury et al., in prep). Among these genes, we found an EST encoding a neuropeptide Y related...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00083/19424/17035.pdf

Genetic structure of a commercially exploited bivalve, the great scallop Pecten maximus, along the European coasts
Provedor de dados: 5
Autores: Morvezen, Romain; Charrier, Gregory; Boudry, Pierre; Chauvaud, Laurent; Breton, Florian; Strand, Oivind; Laroche, Jean.
The great scallop Pecten maximus is harvested in several European countries and fisheries targeting this species are severely regulated by fishing quotas. In addition, hatchery-based population enhancement has been developed in some countries to provide alternative or complementary production. The genetic structure of wild populations of P. maximus and the potential impact of aquaculture on the genetic diversity of this species remains poorly documented. In this study, we explored the genetic structure of P. maximus using 12 microsatellite markers, considering 14 populations sampled from Galicia (Spain) to the North of Norway, and one population of Pecten jacobaeus (L., 1758) from the Lion Gulf (Mediterranean Sea). Results indicated a clear differentiation...
Tipo: Text
Palavras-chave: Great scallop; Pecten maximus; Microsatellites; Population genetics; Aquaculture.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00311/42251/41692.pdf

Effects of bioactive extracellular compounds and paralytic shellfish toxins produced by Alexandrium minutum on growth and behaviour of juvenile great scallops Pecten maximus
Provedor de dados: 5
Autores: Borcier, Elodie; Morvezen, Romain; Boudry, Pierre; Miner, Philippe; Charrier, Gregory; Laroche, Jean; Hegaret, Helene.
Dinoflagellates of the genus Alexandrium are a major cause of harmful algal blooms (HABs) that have increasingly disrupted coastal ecosystems for the last several decades. Microalgae from the genus Alexandrium are known to produce paralytic shellfish toxins (PST) but also bioactive extracellular compounds (BEC) that can display cytotoxic, allelopathic, ichtyotoxic or haemolytic effects upon marine organisms. The objective of this experimental study was to assess the effects of PST and BEC produced by A. minutum upon juvenile great scallops Pecten maximus. Scallops were exposed for one week to two different strains of A. minutum, the first producing both PST and BEC and the second producing only BEC. Escape response to starfish, daily shell growth,...
Tipo: Text
Palavras-chave: Harmful Algal Bloom (HAB); Alexandrium minutum; Pecten maximus; Paralytic Shellfish Toxins (PST); Extracellular compounds; Behaviour; Histology.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00368/47878/47893.pdf

Residual genetic variability in domesticated populations of the Pacific blue shrimp (Litopenaeus stylirostris) of New Caledonia, French Polynesia and Hawaii and some management recommendations
Provedor de dados: 5
Autores: Goyard, Emmanuel; Arnaud, Sophie; Vonau, Vincent; Bishoff, Vincent; Mouchel, Olivier; Pham, Dominique; Wyban, Jim; Boudry, Pierre.
The Latin American shrimp Litopenaeus stylirostris was introduced in three different Pacific islands (Tahiti, New Caledonia via Tahiti, and Hawaii) and hatchery-propagated for 7-25 generations to develop shrimp farming based on these domesticated stocks. Three microsatellite markers have been used in an attempt to assess the genetic bases of the populations available to start a selective breeding program. The comparison of eight hatchery stocks (five New Caledonian, two Hawaiian and one Tahitian stocks) and one wild Ecuadorian population showed a much lower variability in the domesticated stocks than in the wild population, especially in New Caledonia and Tahiti (2-3.7 vs. 14-27 alleles per locus; 20-60% vs. 90% expected heterozygosity). The Tahitian and...
Tipo: Text
Palavras-chave: Microsatellite marker; Genetic variability; Domestication; Litopenaeus stylirostris; Shrimp.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-596.pdf

Impact de la dissémination accidentelle d'huîtres tétraploïdes : potentiel de dispersion et succès reproducteur
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Cornette, Florence; Brizard, Raphael; Sharbel, Tim; Boudry, Pierre.
L'ostréiculture française réalise une production très largement extensive, basée sur le recrutement de juvéniles dans le milieu naturel et la production sur des sites côtiers. Ainsi, l’huître est l’une des rares espèces cultivées à être encore considéré par le consommateur comme un produit alimentaire totalement « naturel ». L'utilisation de la polyploïdisation a permis d'ouvrir une nouvelle voie dans cette filière, en permettant d'obtenir des animaux à croissance plus rapide, plus homogènes et stériles. Cette dernière caractéristique est un gage de qualité gustative et permet aussi de positionner ce produit en priorité sur les mois en "r" traditionnellement bannis à cause de la présence de laitance. Le but principal de la triploïdisation a été atteint:...
Tipo: Text
Palavras-chave: Génétique; Triploïdisation; Tétraploïde; Impact; Environnement; Bivalvia; Crassostrea gigas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00043/15460/12835.pdf

Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas
Provedor de dados: 5
Autores: Guevelou, Eric; Huvet, Arnaud; Galindo-sanchez, Clara E.; Milan, Massimo; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the...
Tipo: Text
Palavras-chave: AMP-activated protein kinase; Crassostrea gigas; Gametogenesis; Marine bivalve; Reproduction.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00168/27911/26470.pdf

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and...
Tipo: Text
Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf

Etudes des bases génétiques et de la varibailité des caractères physiologiques impliqués dans la croissance chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Collet, Bertrand; Boudry, Pierre; Bougrier, Serge; Heurtebise, Serge; Phelipot, Pascal; Ledu, Christophe; Morand, Berenice; Heral, Maurice; Gerard, Andre.
A European programme (GENEPHYS) was launched in 1996 to study the genetic basis and variability of physiological characters involved in growth in Crassostrea gigas. This research programme was a prerequisite for any genetic selection programme on physiological functions. Research groups from Ireland, England, Greece and France are involved in this project and participate in genetic analyses (allozymes and microsatellites) and physiological analyses (ecophysiology, enzymology, stress proteins). Ifremer at La Tremblade was responsible for producing the biological material while Ifremer-CREMA (human-influenced marine ecosystems research centre) was responsible for carrying out non-destructive physiological analyses.
Tipo: Text
Palavras-chave: Sélection génétique; Croissance; Crassostrea gias; Génétique.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3243.pdf

Stress response of farmed European abalone reveals rapid domestication process in absence of intentional selection
Provedor de dados: 5
Autores: Lachambre, Sebastien; Day, Rob; Boudry, Pierre; Huchette, Sylvain; Rio-cabello, Antoine; Fustec, Tirnothee; Roussel, Sabine.
Farming, and thus the domestication of Haliotis tuberculata, began recently. We compared the responses of unselected farmed and wild abalone to stressors that occur on farms. The aim was to determine if the farm environment had induced differences in the behavioural or physiological performances of the abalone. Thirty hatchery-born 3.5 year-old abalone and thirty wild ones were reared under standard farm conditions for 6 months and characterised for 19 traits related to growth, survival, behaviour and immunology. Behavioural and immunological responses to stressors differed between the two stocks. Farmed abalone retracted and swivelled less in reaction to a finger contact. Phagocytosis efficiency was reduced by shaking in abalone from both origins, but the...
Tipo: Text
Palavras-chave: Abalone aquaculture; Domestication syndrome; Behavioural adaptation; Responses to stress; Immune response; Farm stressors.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00395/50598/53725.pdf

Summer mortality in juvenile cupped oysters : response to selection in the field and in laboratory
Provedor de dados: 5
Autores: Degremont, Lionel; Bedier, Edouard; Soletchnik, Patrick; Ropert, Michel; Samain, Jean-francois; Boudry, Pierre.
The French project "Morest", coordinated by IFREMER, aims to a better understanding of the causes of summer mortalities in juvenile oysters by a multidisciplinary approach. Within this program, a first generation (G1) was bred in 2001 to assess to what extent genetic variability exists for this trait in French populations. 17 half-sib families (HSF) including 44 full-sib families (FSF) were obtained in the G1, and each FSF was reared in three sites along the French coast during the summer period. Significant differences in survival were observed among HSF, clearly indicating a genetic basis for survival. Estimation of heritability for survival was very high: 0.81 &amp;#61617; 0.29. In 2002, 3 "good" HSF and 3 "bad" HSF were selected on their survival...
Tipo: Text
Palavras-chave: Selection; Genetic; Mortalities; Crassostrea gigas; Oyster.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3439.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea : hybridation et conservatoire de souches. Contrat Région Poitou-Charentes 1999 - Convention 99 RPC-A-203 "Génétique"
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Dans le cadre du plan Etat-Région 1994-1998, un programme de conservatoire de souches a été initié au Laboratoire IFREMER de La Tremblade. Ce conservatoire a pour objectif d'acclimater différentes espèces ou souches d'huîtres creuses (genres Crassostrea et Saccostrea) afin d'acquérir des connaissances sur ces espèces et d'évaluer l'intérêt qu'elles pourraient présenter pour l'ostréiculture locale. L'importation d'espèces étrangères sur les côtes françaises est désormais strictement réglementée: la dissémination d'une nouvelle espèce peut se révéler néfaste d'un point de vue écologique et nuire aux productions existantes. De plus, ces huîtres peuvent être porteuses de pathogènes qui, s'il venaient à se répandre dans le milieu, pourraient toucher les stocks...
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Croisement; Crassostrea gigas; Crassostrea ariakensis; Crassostrea sikamea; Hybridation.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00044/15514/12901.pdf

Rapport d’activité 2015 - Unité Physiologie Fonctionnelle des Organismes Marins
Provedor de dados: 5
Autores: Boudry, Pierre; Alunno-bruscia, Marianne; Zambonino, Jose-luis; Le Roux, Frederique.
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00346/45767/45409.pdf

Historique, expériences en cours et perspectives du programme de sélection de l'huître plate Ostrea edulis pour la résistance à la bonamiose
Provedor de dados: 5
Autores: Barre, Marc; Naciri, Yamama; Boudry, Pierre; Goyard, Emmanuel; Launey, Sophie; Cochennec, Nathalie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
The Aquaculture and Pathological Genetics (GAP) laboratory at La Tremblade has been monitoring three populations of European flat oyster since 1985 or 1989 under selection for higher survival rates in the presence of the parasite Bonamia ostrea. One selection cycle (1985 population) or two (1989 populations) were carried out by mass selection by placing the oysters for several years in a highly infested zone, and then harvesting spawns from survivors.
Tipo: Text
Palavras-chave: Huîtres plates; Génétique; Selection génétique; Ostrea edulis.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3244.pdf

Gestion des ressources génétiques de l'huître perlière Pinctada margaritifera de Polynésie française : caractérisation génétique des populations et optimisation du recrutement pour l'exploitation perlière
Provedor de dados: 5
Autores: Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre.
Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different...
Tipo: Text
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF

Hybridation naturelle entre les deux sous-espèces d'huîtres creuses Crassostrea gigas et Crassostrea angulata au sud de l'Europe
Provedor de dados: 5
Autores: Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Boudry, Pierre.
Crassostrea angulata and Crassostrea gigas, two cupped oysters of Asian origin (O'Foighil et al, 1998), have been introduced successively in Europe during the XVlth and the XXth century (Grizel et Héral, 1991). The present populations of C. angulata appear to be limited to the south of Spain and Portugal, whereas the distribution of C. gigas in Europe is rising because of the development of oyster farming. The distinction between the two subspecies in based on a RFLP marker of the mitochondrial genome (COI) which shows a strong genetic differentiation between the two subspecies populations (Boudry et al., 1998). On the contrary, the nuclear markers (microsatellites) showed a smaller genetic differentiation between them (Huvet et al, under press). The...
Tipo: Text
Palavras-chave: Microsatellite; Hybridation; Crassostrea angulata; Crassostrea gigas; Huîtres; Génétique.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3282.pdf

Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas
Provedor de dados: 5
Autores: Bigot, Laetitia; Beets, Isabel; Dubos, Marie-pierre; Boudry, Pierre; Schoofs, Liliane; Favrel, Pascal.
Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (CgsNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values...
Tipo: Text
Palavras-chave: Oyster; Crassostrea gigas; SNPF; Energy metabolism; Reproduction; Starvation.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00207/31777/30188.pdf

Morphological and mitochondrial DNA characteristics of two cultured species of cupped-oysters (Bivalvia : Ostreidae) in Hong Kong : towards a significant taxonomic name change
Provedor de dados: 5
Autores: Lam, Katherine; Morton, Brian; Boudry, Pierre; Heurtebise, Serge.
Cultured cupped-oysters species in Hong Kong have rarely been differentiated properly due to similarities in shell characters. This study attempted to characterize putative Crassostrea gigas and Crassostrea ariakensis by morphological and genetic means. Allometric ratios of shell height/length and depth/length were significantly different between the two species. Restriction fragment length polymorphism (RFLP) of a PCR amplified mitochondrial COI (cytochrome oxidase I) fragment was used to examine genetic differentiation between putative C. gigas from Hong Kong and previously studied populations of Crassostrea from Europe, Japan and Taiwan. The two Hong Kong species are not only morphologically, but also genetically different from each other in terms of...
Tipo: Text
Palavras-chave: Oysters; Hong Kong Crassostrea; Characteristics; Genetic population; Crassostrea ariakensis; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2844.pdf

Génomique de l'adaptation de l'huître creuse du Pacifique, Crassostrea gigas, dans le cadre de son expansion géographique
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).  Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf

PERLE - Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis - Rapport final des sous-programmes 2 et 3
Provedor de dados: 5
Autores: Arzul, Isabelle; Baillon, Laury; Boudry, Pierre; Bouget, Jean-francois; Chollet, Bruno; Cochet, Helene; Couraleau, Yann; Dubreuil, Christine; Duchene, Julie; Dutartre, Julie; Garcia, Celine; Heloury, Purotu; Heurtebise, Serge; Huchet, Eve; Hussenot, Mathieu; Langlade, Aime; Ollier, Simon; Omnes, Emmanuelle; Pouvreau, Stephane; Roby, Charlotte; Serpin, Delphine; Viard, Frederique; Wacrenier, Candice; Lapegue, Sylvie.
L’huître plate (Ostrea edulis) est une espèce endémique des côtes européennes, elle est présente depuis des millions d’années. Son aire de répartition est très vaste allant de la Norvège jusqu’au Maroc. Elle est également présente en mer Méditerranée, en mer Noire, mais également au Canada, aux Etats-Unis ou encore en Afrique du Sud, à la suite d'introductions pour des raisons aquacoles. En France, à partir de la fin des années 70, de fortes mortalités ont été constatées sur les bancs naturels et en élevage, faisant rapidement chuter la production de 20 000 tonnes à 2 000 tonnes. Ces mortalités ont été attribuées à deux maladies parasitaires : la marteiliose (due au parasite Marteilia refringens) et la bonamiose (due au parasite Bonamia ostreae). Trente...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00247/35815/34328.pdf

Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Boudry, Pierre; Lapegue, Sylvie.
We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.
Tipo: Text
Palavras-chave: Pacific oyster; Null alleles; EST SSR markers; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6419.pdf

Establishment of a genetic map in the European flat oyster Ostrea edulis L.
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic from European coasts, Atlantic and Mediterranean. It has been exploited since Roman times in Europe. But its production decreased from around 20000 tons in the 1950ies to 1500 tons nowadays because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer initiated a program of selection to produce families of oysters tolerant to Bonamia. In this context, a further step is to identify QTLs of resistance to this parasite. Therefore, a genetic map is first now being built. The establishment of a genetic linkage map will represent the basement for the mapping of QTLs, with the ultimate objective to implement marker-assisted selection in O....
Tipo: Text
Palavras-chave: Genetic map; Ostrea edulis; Genetic; European flat oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3472.pdf

In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement
Provedor de dados: 5
Autores: Foulon, Valentin; Boudry, Pierre; Artigaud, Sebastien; Guérard, Fabienne; Hellio, Claire.
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Pediveliger larvae; Bioadhesive; Transcriptome.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00475/58677/61172.pdf

Genetic and reproduction strategy in Crassostrea gigas mortality
Provedor de dados: 5
Autores: Moal, Jeanne; Bedier, Edouard; Fleury, Pierre-gildas; Langlade, Aime; Le Coguic, Yvette; Degremont, Lionel; Boudry, Pierre; Le Coz, Jean-rene; Pouvreau, Stephane; Enriquez-diaz, M.; Lambert, Christophe; Quere, Christele; Soudant, Philippe; Samain, Jean-francois.
Bi-parental families were produced in hatchery and tested in the field in 2001. Two sets of 5 families were constituted, selected on their high (R) and low (S) survival. These two sets were reared in Brittany from March to November 2002. Samplings were performed twice a month to obtain data on biometry, survival, reproductive cycle, biochemical composition, adenylate energy charge, hemolymph parameters (ions and defense system) and muscle strength. "R" and "S" oysters exhibited different reproductive effort and spawning strategy. "R" oysters allocated less energy in gonad than "S" ones and presented a complete spawning at the end of August contrary to the "S" which spawns partially. Mortality started in July when the seawater temperature reached 19 degree...
Tipo: Text
Palavras-chave: Summer; Reproduction; Oyster culture; Mortality; Genetic diversity; Commercial species; Breeding.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3345.pdf

Molecular markers as a tool to study genetic resources in oysters
Provedor de dados: 5
Autores: Boudry, Pierre.
Despite the world economic importance of oyster farming, genetic improvement has not yet had a great impact on these species. Oyster farming is traditionnally based on wild stocks, whose natural populations are often overexploited, and frequently does not fulfil market demande....
Tipo: Text
Palavras-chave: Molecular markers; Genetic resources; Oysters.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3212.pdf

Mortalité du naissain d’Huître creuse Crassostrea gigas dans l’étang de Thau en 2009
Provedor de dados: 5
Autores: Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Malet, Nathalie; Pastoureaud, Annie; Munaron, Dominique; De Lorgeril, Julien; Bachere, Evelyne; Vaquer, Andre; Huvet, Arnaud; Corporeau, Charlotte; Normand, Julien; Boudry, Pierre; Moal, Jeanne; Quere, Claudie; Quilien, Virgile; Daniel, Jean-yves; Pepin, Jean-francois; Saulnier, Denis; Gonzalez, Jean-louis.
This study was conducted in the Mediterranean area during a mass mortality event of Pacific oyster Crassostrea gigas. The objectives of this work were to increase our understanding of the mass mortality phenomena in Pacific oysters, to identify risky cultivation practises and to propose mitigation strategies. In the first part of this work, we followed environmental parameters of the Thau lagoon in relation with mortality events. Then, we compared mortality and growth parameters of oysters as a function of their origins, their development stages and cultivation sites in relation with energetic, reproduction and pathogens. Finally, we conducted transfer experiments where oysters maintained free of mortality in open sea were transferred in the Thau lagoon...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11354/7927.pdf

Population genetics of the European flat oyster (Ostrea edulis) : from larvae to populations
Provedor de dados: 5
Autores: Boudry, Pierre; Launey, Sophie; Diaz-almela, E.; Naciri-graven, Yamama; Ledu, Christophe; Mira, Sara; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
Nowadays, PCR-based DNA markers allow the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European flat oyster (Ostrea edulis) populations has aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species.  An Atlantic/Mediterranean differentiation pattern was observed. Results were congruent between allozymes, microsatellites and mitochondrial DNA and  support an isolation by distance model. High variability was observed in the breadth of diversity between populations using a mitochondrial marker, this highlights the small effective population sizes in some locations. A 10-fold quantitative difference was observed in Fst...
Tipo: Text
Palavras-chave: Larvae; Reproduction; Genetic; Ostrea edulis; European flat oyster; Population genetics.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-3493.pdf

Etude de la croissance et de l'activité écophysiologique des huîtres creuses Crassostrea gigas, Crassostrea angulata et de leurs hybrides en système contrôlé
Provedor de dados: 5
Autores: Haure, Joel; Huvet, Arnaud; Palvadeau, Hubert; Nourry, Max; Martin, Jean-louis; Boudry, Pierre; Penisson, Christian.
A comparative study of the growth of the oysters Crassostrea gigas, Crassostrea angulata and their hybrids was carried out in 1999 in system controlled with contribution of the micro alga Skeletonema costatum. The results showed significant differences of the pondéral growth of the four studied progenies. The animals C. gigas had growth rates 1.4 times greater than C. angulata oysters. The hybrid progenies were shown more powerful than Portuguese oysters but are not different from the Japanese oysters. The ecophysiologic study showed that the averages of filtration and oxygen uptake, obtained by means of a three hours recording, cannot differentiate the four studied progenies. On the other hand, the calculation of the FTA (Feeding Time Activity) allowed to...
Tipo: Text
Palavras-chave: Skeletonema costatum; Hybrids; Crassostrea angulata; Crassostrea gigas; Ecophysiology; Growth; Skeletonema costatum; Hybrides; Crassostrea angulata; Crassostrea gigas; Ecophysiologie; Croissance.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/rapport-1636.pdf

Les surmortalités des naissains d’huîtres creuses, Crassostrea gigas : acquis des recherches en 2010
Provedor de dados: 5
Autores: Cochennec-laureau, Nathalie; Baud, Jean-pierre; Pepin, Jean-francois; Benabdelmouna, Abdellah; Soletchnik, Patrick; Lupo, Coralie; Garcia, Celine; Arzul, Isabelle; Boudry, Pierre; Huvet, Arnaud; Pernet, Fabrice; Bachere, Evelyne; Bedier, Edouard; Petton, Bruno; Gaussem, Florian; Stanisiere, Jean-yves; Degremont, Lionel.
Depuis 2008, la filière ostréicole française doit faire face à des surmortalités exceptionnelles des naissains d’huîtres creuses, Crassostrea gigas, (huîtres de moins d’un an) comprises entre 60 et 90% dans tous les sites d’élevage. Des cas de mortalité similaires ont été décrits en Irlande, dans les îles anglo-normandes, au Portugal en 2009 et au Royaume-Uni en 2010. Récemment deux épisodes de mortalité, associés à la présence d’un virus OsHV1 μvar (ou un variant très proche) ont été également décrits en Nouvelle Zélande (décembre 2010) et en Australie (janvier 2011) soulignant la vulnérabilité de la filière ostréicole dans le monde. L’objectif de ce document est de faire un point sur les recherches menées depuis l’apparition de ce phénomène et de...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14423/11719.pdf

Mise en évidence expérimentale de la variance du succès reproducteur chez l'huître creuse Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Kotoulas, G.; Bonhomme, Francois.
Oysters, like many other marine species, are characterized by very high fertilities. Natural or hatchery populations, often present very low Ne/N ratios (Hedgecock, 1994). It can be explained by very high variances of their breeding success (Li and Hedgecock, 1998), the effective population size being estimated through the temporal variance of the allelic frequencies in natural populations (Hedgecock et al., 1992) or, more directly, through the estimated number of founders in hatchery populations (Launey, 1998). This document is a presentation of the results of a number of experiments, realised in the scope of the European program "GENEPHYS" (FAIR 95-421), and using microsatellite markers to analyse the parental contributions in the flat oyster Crassostrea...
Tipo: Text
Palavras-chave: Microsatellite; Succès reproducteur; Contributions parentales; Génétique; Crassostrea gigas; Huîtres.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3454.pdf

Integrative Study of Physiological Changes Associated with Bacterial Infection in Pacific Oyster Larvae
Provedor de dados: 5
Autores: Genard, Bertrand; Miner, Philippe; Nicolas, Jean-louis; Moraga, Dario; Boudry, Pierre; Pernet, Fabrice; Tremblay, Rejean.
Background:  Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus. Findings:  Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00144/25504/23662.pdf

Scallop larval survival from erythromycin treated broodstock after conditioning without sediment
Provedor de dados: 5
Autores: Holbach, Marine; Robert, Rene; Boudry, Pierre; Petton, Bruno; Archambault, Philippe; Tremblay, Rejean.
Pathogenic bacteria are known to be one of the main factors affecting Pecten maximus larval survival in hatcheries. As a result, juvenile production often relies on the use of antibiotics during larval culture. However, limitations of the usage of chemicals such as chloramphenicol in aquaculture have been reinforced due to their negative environmental impact and alternatives are accordingly needed. Thus, the importance of bacterial transfer from oocytes to larvae has been studied here as well as procedures to limit larval mortality in P. maximus. In order to reduce bacterial contamination during larval development, we focused on two periods, broodstock conditioning and post-fertilization. The animals were conditioned for 2 months with two erythromycin...
Tipo: Text
Palavras-chave: Scallop; Broodstock conditioning; Larval survival; Vibriosis.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00240/35171/33675.pdf

Comparative study of shell shape and muscle scar pigmentation in the closely related cupped oysters Crassostrea angulata, C. gigas and their reciprocal hybrids
Provedor de dados: 5
Autores: Batista, Frederico; Ben-hamadou, Radhouan; Fonseca, Vera; Taris, Nicolas; Ruano, Francisco; Reis-henriques, Maria; Boudry, Pierre.
The taxonomic status of the cupped oysters Crassostrea angulata and C. gigas has received considerable attention in the last decades. Based on larval shell morphology, experimental hybridization, allozymes and nuclear DNA studies several authors have considered these two taxa as being synonymous. However, mitochondrial data showed clear genetic differences between the two taxa. In addition, microsatellite-based studies and cytogenetic studies have also provided evidence that supports their differentiation. Considerable differences have also been observed at the phenotypic level in terms of growth rate and ecophysiological parameters. In the present study, C. angulata from Sado estuary (Portugal) and C. gigas from Seudre estuary (France) were collected and...
Tipo: Text
Palavras-chave: Crassostrea gigas; Crassostrea angulata; Hybridization; Shell pigmentation; Shell morphology.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3904.pdf

Etude comparative de la croissance et de l'activité écophysiologique des huîtres creuses Crassostrea gigas, Crassostrea angulata et des hybrides
Provedor de dados: 5
Autores: Haure, Joel; Huvet, Arnaud; Palvadeau, Hubert; Nourry, Max; Penisson, Christian; Boudry, Pierre; Martin, Jean-louis.
A comparative study of the growth performances in cupped oysters Crassostrea gigas, Crassostrea angulata and their hybrids was conducted from March to October 1999 in a controlled farm with phytoplankton (Skeletonema costatum) input. The individuals, of the same age, were from genitors which had been identified through a marker analysis of the mitochondrial genome (Boudry et al, 1998). As a consequence, two pure offspring were obtained through the crossing of Portuguese oyster C. angulata (AA) genitors and the crossing of Japanese oyster C. gigas (GG) genitors. Two other offsprings were produced through the hybrid crossing of C. gigas female genitors with C. angulata (GA) male genitors on one hand, and C. angulata female genitors with C. gigas (AG) male...
Tipo: Text
Palavras-chave: Géniteur; Génétique; Performances croissance; Crassostrea gigas; Crassostrea angulata; Huîtres.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3281.pdf

Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) (slides)
Provedor de dados: 5
Autores: Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective...
Tipo: Text
Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf

Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - Second Progress Report 1st January-31st December 1997 - Contract n° FAIR 95-421 Commission of the European Communities
Provedor de dados: 5
Autores: Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkins, N. P.; Zouros, Eleftherios; Bonhomme, Francois; Buestel, Dominique; Samain, Jean-francois.
The UE project "GENEPHYS", initiated by the IFREMER laboratory GAP-La Tremblade (Génétique Aquaculture et Pathologie) and CREMA-L'Houmeau (Centre de Recherche en Ecologie marine et Aquaculture), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are : 1. To express the maximum variability of growth, both at the larval stage and at the adult stage, in a oyster population originating from parents of various origins. 2. To check the persistence over the time, of growth performance and related physiological functions. 3. To explain...
Tipo: Text
Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00050/16150/13633.pdf

Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L.
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boudry, Pierre; Mira, Sara; Taris, Nicolas; Bonhomme, Francois.
In order to understand better some fundamental aspects of the bivalve biology, one has to pay attention to the variance of their breeding success, the time structure of the populations and their effective size. An abundant literature shows the existence of a heterozygote deficit as well as positive relationships between heterozygoty and fitness components. This last point is the subject of explanatory hypotheses which have never been clearly decided between until now. One of these hypotheses is the possible existence of a certain degree of inbreeding among those populations. This is only possible in the case of separated colonies coming from a small amount of spawners, thus creating blood relations between individuals recruited from the same area, and...
Tipo: Text
Palavras-chave: Reproducteur; Variabilité génétique; Génétique population; Diversité génétique; Ostrea edulis; Huître plate.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-3494.pdf

Relationship between pre- and post-metamorphic growth in the Pacific oyster Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Collet, Bertrand; Boudry, Pierre; Thebault, Anne; Heurtebise, Serge; Morand, Berenice; Gerard, Andre.
Twenty male and 20 female parental oysters, originating from four sites located along the French Atlantic coast, were crossed together. The 400 crosses were performed separately and then pooled to give a batch of larvae with a large genetic base. Successive sieving after 17 days at 23°C enabled the separation of the largest larvae from the batch. These larvae (i.e., sieving groups) were left to metamorphose and fix onto flat PVC collectors changed daily. Four groups of larvae representing distinctly different growth rates were successively separated during the attachment and metamorphosis of the whole population. This lasted 12 days, from the 17th day to the 29th after fertilisation. A part of each sieving group was settled separately as replicates on...
Tipo: Text
Palavras-chave: Settlement; Metamorphosis; Crassostrea gigas; Growth.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-468.pdf

Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy
Provedor de dados: 5
Autores: Dheilly, Nolwenn M.; Jouaux, Aude; Boudry, Pierre; Favrel, Pascal; Lelong, Christophe.
Background: Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named "3n beta'') yet a low but significant proportion show an advanced gametogenesis (named "3n alpha''). These oysters thus constitute an interesting model to study the effect of triploidy on germ cell development. We used microarrays to compare the gonad transcriptomes of diploid 2n and the abovementioned triploid 3n beta and 3n alpha male and female oysters throughout gametogenesis. Results: All triploids displayed an upregulation of genes related to DNA repair and apoptosis and a downregulation of genes...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00239/34992/33538.pdf

Bilan des « Journées Surmortalité des huîtres creuses, Crassostrea gigas » du Programme P7 « Aquaculture Durable » des 8 et 9 décembre 2009
Provedor de dados: 5
Autores: Cochennec-laureau, Nathalie; Baud, Jean-pierre; Bedier, Edouard; Boudry, Pierre; Huvet, Arnaud; Nicolas, Jean-louis; Pepin, Jean-francois; Petton, Bruno.
Ce bilan comprend deux parties : la première est dédiée à une synthèse des travaux présentés lors des journées « Surmortalités des huîtres creuses, Crassostrea gigas » les 8 et 9 décembre 2009 et, la seconde partie, à une proposition d'un projet de recherche spécifique 2010-2012 en complément des propositions de « sortie de crise ».  Contexte Les mortalités estivales de l'huître creuse, Crassostrea gigas, sont décrites depuis une vingtaine d'années en France mais également au Japon, en Corée, aux USA et en Australie. Ces phénomènes ou « syndrome des mortalités estivales » ont fait l'objet d'une étude pluridisciplinaire en France entre 2001 et 2006 (Défi MOREST). Toutefois, en 2008 et 2009, l'ostréiculture française a du faire face à deux épisodes de...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/2010/acte-7393.pdf

Mark-recapture cloning: a straightforward and cost-effective cloning method for population genetics of single-copy nuclear DNA sequences in diploids
Provedor de dados: 5
Autores: Bierne, Nicolas; Tanguy, A; Faure, Michel; Faure, B; David, E; Boutet, I; Boon, E; Quere, N; Plouviez, S; Kemppainen, P; Jollivet, D; Moraga, D; Boudry, Pierre; David, P.
We describe a simple protocol to reduce the number of cloning reactions of nuclear DNA sequences in population genetic studies of diploid organisms. Cloning is a necessary step to obtain correct haplotypes in such organisms, and, while traditional methods are efficient at cloning together many genes of a single individual, population geneticists rather need to clone the same locus in many individuals. Our method consists of marking individual sequences during the polymerase chain reaction (PCR) using 5'-tailed primers with small polynucleotide tags. PCR products are mixed together before the cloning reaction and clones are sequenced with universal plasmid primers. The individual from which a sequence comes from is identified by the tag sequences upstream...
Tipo: Text
Palavras-chave: Sequence; Population genetics; High throughput allele recognition; DNA polymorphism.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-4222.pdf

Additive transcriptomic variation associated with reproductive traits suggest local adaptation in a recently settled population of the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Sussarellu, Rossana; Huvet, Arnaud; Lapegue, Sylvie; Quillen, Virgile; Lelong, Christophe; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Background Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe.  Methods To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60).  Results A female-biased sex-ratio and a higher condition index were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Adaptation; Invasiveness; Fertility; Sex-ratio; Transcriptome; Microarray.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00287/39829/38343.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu'il existe, chez l'huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Anomalies; Aneuploïdie; Atrazine; Huîtres creuses; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31013/29417.pdf

Diversité génétique chez les huîtres creuses du genre Crassostrea : quelles ressources pour quelle amélioration des productions de C; gigas?
Provedor de dados: 5
Autores: Boudry, Pierre.
Despite the economic importance of oyster farming, knowledge about genetic resources of these species is still limited. The objectives of my research were to provide further information about (1) taxomomy, phylogeny and phylogeography of Crassostrea oysters, (2) to compare C. gigas and C. angulata strains of various geographic origins and (3) to study phenotypic and genetic variation for traits of aquacultural interest in C. gigas. Studies based on DNA polymorphism and chromosomes allowed to improve or correct identification, geographic range and phylogeographic relationships among several species. The comparison of various strains of C. gigas and C. angulata, both in laboratory and field conditions, revealed significant differences among taxa and the...
Tipo: Text
Palavras-chave: Crassostrea  gigas; Cupped oysters; Genetic improvement by selection; Variability; Strains testing; Phylogeny; Genetic resources; Crassostrea gigas; Huîtres creuses; Amélioration par la sélection; Variabilité; Testage de souches; Phylogénie; Ressources génétiques.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/these-3354.pdf

Study of the reproductive potential of triploid oysters
Provedor de dados: 5
Autores: Normand, Julien; Boudry, Pierre; Cornette, Florence; Ledu, Christophe.
Reproduction was studied in triploid Crassostrea gigas, by crossing gametes from diploid and triploid parents.Triploids are able to produce viable gametes and their resulting progeny consisted in diploids, aneuploids and polyploids. Gametes from triploid parents frequently led to abnormal polar body formation, even before caryogamy occurred. A high frequency of aneuploids was observed, related to the missegregation of chromosomes during meiosis in triploids. This might be due to the observed incomplete synapsis in triploid eggs during Prophase I. The proportion of aneuploids decreased during larval development, reflecting differential mortality between ploidy levels. Due to limited gonadic development and high larval mortality rates, the reproductive...
Tipo: Text
Palavras-chave: Triploid; Genetic; Oysters; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3319.pdf

Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review
Provedor de dados: 5
Autores: Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf

From Crassostrea gigas oyster larvae adhesion studies to potential biotechnological development of marine adhesives
Provedor de dados: 5
Autores: Foulon, Valentin; Boudry, Pierre; Guerard, Fabienne; Hellio, Claire.
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00373/48449/48717.pdf

Disruption of amylase genes by RNA interference affects reproduction in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Beguel, Jean-philippe; Pereira Cavaleiro, Nathalia; Thomas, Yoann; Quillien, Virgile; Boudry, Pierre; Alunno-bruscia, Marianne; Fabioux, Caroline.
Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15...
Tipo: Text
Palavras-chave: Alpha-Amylase; DsRNA; Gametogenesis; Marine bivalve; Dynamic energy budget.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00271/38239/36422.pdf

Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). II. Response to selection for survival and its influence on growth and yield
Provedor de dados: 5
Autores: Degremont, Lionel; Bedier, Edouard; Boudry, Pierre.
Response to divergent selection for "high" and "low" survival during the summer period, from July to October, was investigated in juvenile (six-month-old) Pacific oysters, Crassostrea gigas, by producing two sets of progenies in 2002 (Generation 2) and three sets of progenies in 2003 (Generation 3). A strict between-family approach was used and resistance of these selected progenies to summer mortality was assessed in three sites along French coasts, to determine their response to selection and estimate realized heritability of the trait. A significant difference in survival was observed between the "high" and "low" selected groups at all sites for all sets of progenies, indicating a significant genetic component. High realized heritabilities for survival...
Tipo: Text
Palavras-chave: Crassostrea gigas; Heritability; Juveniles; Summer mortality; Survival; Growth.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/2009/publication-6950.pdf

A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments
Provedor de dados: 5
Autores: Mccombie, Helen; Ledu, Christophe; Phelipot, Pascal; Lapegue, Sylvie; Boudry, Pierre; Gerard, Andre.
We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However,...
Tipo: Text
Palavras-chave: Oyster breeding; Gynogenesis; Polar body; Cytochalasin B; Polyploidy; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-632.pdf

What role for genomics in fisheries management and aquaculture?
Provedor de dados: 5
Autores: Wenne, Roman; Boudry, Pierre; Hemmer-hansen, Jakob; Lubieniecki, Krzysztof P.; Was, Anna; Kause, Antti.
The development and application of genomics has been facilitated in a number of fields by the availability of new methodologies and tools, such as high throughput DNA sequencing and complementary DNA (cDNA) microarrays. Genomic tools are already used in research on commercially important fish and shellfish species. Thousands of expressed sequence tags (EST) are now available for some of these species, and the sequencing of complete genomes of tilapia, cod, salmonids, flatfishes, sea bass and Pacific oyster has been proposed. Microarray technology through simultaneous analysis of the expression of thousands of genes allows the identification of candidate genes involved in the function of multiple physiological, morphological and behavioural traits of...
Tipo: Text
Palavras-chave: Oyster; Fish; Quantitative trait loci; Mariculture; Fisheries; Genetics; Genomics.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2927.pdf

Response to unintentional selection for faster development associated with inbreeding depression in Crassostrea gigas larvae, resulting from a broodstock selective breeding plan
Provedor de dados: 5
Autores: Taris, Nicolas; Batista, Frederico; Marissal, Eric; Boudry, Pierre.
Direct and indirect consequences of selective breeding in marine bivalves still remain largely unexplored. For species with two-phase life cycle, like the Pacific oyster (Crassostrea gigas), most studies have focused on juvenile and adult stages, but relatively few have focused on juvenile and adult stages, but relatively few have considered the larval stage, especially in a domestication context. We assessed the impact of hatchery practices on larval traits, notably on larval growth (due to the culling of slow growing larvae), by the study of larval progenies. Larvae originating from crosses using parental oysters both from natural beds and from hatchery broodstock which had been selected for adult growth and shell shape for seven generations. A set of...
Tipo: Text
Palavras-chave: Inbreeding depresion; Larvae stage; Genetic variability; Hatchery; Domestication; Genetic; Crassostrea gigas; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3337.pdf

Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas
Provedor de dados: 5
Autores: Boullot, Floriane; Castrec, Justine; Bidault, Adeline; Dantas, Natanael; Payton, Laura; Perrigault, Mickael; Tran, Damien; Amzil, Zouher; Boudry, Pierre; Soudant, Philippe; Hegaret, Helene; Fabioux, Caroline.
Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed...
Tipo: Text
Palavras-chave: Crassostrea gigas; Sodium channel; Alternative splicing; Alexandrium minutum; Paralytic shellfish toxins.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00368/47877/47892.pdf

Evidence for natural hybrtidisation between the two oyster sub-species Crassostrea gigas and Crassostrea angulata in Southern Europe
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie.
Crassostrea angulata and Crassostrea gigas, two cupped oyster taxa of Asian origin (O'Foighil et al, 1998), were successively introduced into Europe  during the XVIth  and the XXth century respectively (Héral and Grizel, 1991). To date, populations presumed to be C. angulata remain only in the south of Spain and Portugal, while the geographic range of C. gigas is expending, notably due to aquaculture. The two taxa can be distinguished by RFLP analysis of the mitochondrial Cytochrome Oxydase I locus (COI).  This marker revealed strong genetic differentiation between Asian populations of the two taxa (Boudry et al., 1998). In contrast, nuclear markers showed a much lower genetic differentiation (Huvet et al, in press). The absence of any reproductive barrier...
Tipo: Text
Palavras-chave: Microsatellite; Genetic; Crassostrea angulata; Crassostrea gigas; Oysters.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3453.pdf

Bonamia-like parasite found in the Suminoe oyster Crassostrea rivularis reared in France
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production. Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-Like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea....
Tipo: Text
Palavras-chave: Crassostrea rivularis; Suminoe oyster; Bonamiosis; Bonamia-like parasite.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00446/55795/62652.pdf

Impact of the reproduction dynamics on genetic variation in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to pursue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period; (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females; (3) the variance of individual...
Tipo: Text
Palavras-chave: Ostrea edulis; Oyster; Sexual reproduction; Population dynamics; Oyster culture; Inbreeding; Heterosis; Genetic isolation; Genetic diversity; Breeding success; Biological fertilization.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3436.pdf

Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status
Provedor de dados: 5
Autores: De Decker, Sophie; Normand, Julien; Saulnier, Denis; Pernet, Fabrice; Castagnet, Sophie; Boudry, Pierre.
Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohisto-chemical analyses showed the affinity of Vibrio...
Tipo: Text
Palavras-chave: Experimental vibriosis; Crassostrea gigas; Vibrio; Survival distribution function; Triploidy; Reproductive effort.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00030/14108/11368.pdf

Bonamia-ostreae induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas. Contrat Région Poitou-Charentes 2000-2006. Convention 99
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00043/15473/12852.pdf

Characterization of ten microsatellite loci in the blue mussel Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy R.
Mussels of the genus Mytilus are ecologically and commercially important worldwide, and they form hybrid complexes where their distributions overlap. Allozyme and nuclear markers have bee It Used to investigate their genetics over many years, but successful development of reliable highly valuable microsatellite markers has lagged be hind other shellfish species. We have developed and characterized tell novel microsatellite loci that amplify reliably for the blue mussel Mytilus edulis. The number of alleles among 30 individuals from a wild population (Menai Strait, North Wales UK) ranged between 9 and 29 and the observed heterozygosity between 0.300 and 0.954. Significant heterozygote deficiencies against the Hardy-Weinberg model were observed at 6 out of...
Tipo: Text
Palavras-chave: Mytilus edulis; Microsatellites; Universal tailed-primer labeling; Null alleles; Mussels.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00002/11275/7841.pdf

Effects of age and environment on survival of summer mortality by two selected groups of the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Degremont, Lionel; Boudry, Pierre; Ropert, Michel; Samain, Jean-francois; Bedier, Edouard; Soletchnik, Patrick.
This study investigated the effects of age (first or second summer of life) and environment (three study sites on French coasts) on summer mortality in Crassostrea gigas, using three generations of oysters produced during the Morest project. For each generation, two groups of oysters, selected either as 'resistant' (R) or 'susceptible' (S) to summer mortality at the age of 6 months in Rivière d'Auray (RA, South Brittany), were evaluated. In Ronce (Marennes-Oléron Bay) and RA, R oysters had low mortality during their first and second summers, while mortality was higher during the second summer in Baie des Veys (BDV, Normandy). In RA, S oysters exhibited higher mortality during their first summer than during their second, while the opposite result was found...
Tipo: Text
Palavras-chave: Summer mortality; Age; Environment; Genetic; Crassostrea gigas.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/2009/publication-6972.pdf

Phylogeographic study of the dwarf oyster, Ostreola stentina, from Morocco, Portugal and Tunisia: evidence of a geographic disjunction with the closely related taxa, Ostrea aupouria and Ostreola equestris
Provedor de dados: 5
Autores: Lapegue, Sylvie; Ben Salah, Ines; Batista, Frederico; Heurtebise, Serge; Neifar, L; Boudry, Pierre.
Despite the economic importance of oysters due to the high aquaculture production of several species, the current knowledge of oyster phylogeny and systematics is still fragmentary. In Europe, Ostrea edulis, the European flat oyster, and Ostreola stentina, the Provence oyster or dwarf oyster, are both present along the European and African, Atlantic and Mediterranean, coasts. In order to document the relationship not only between O. stentina and O. edulis, but also with the other Ostrea and Ostreola species, we performed a sequence analysis of the 16S mitochondrial fragment (16S rDNA: the large subunit rRNA-coding gene) and the COI fragment (COI: cytochrome oxidase subunit I). Oysters were sampled from populations in Portugal (two populations), Tunisia...
Tipo: Text
Palavras-chave: Tunisia; Portugal; Marocco; Ostreola stentina; Ostreola equestris; Ostrea aupouria; Phylogeographic study.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1912.pdf

A first generation genetic linkage map of the European flat oyster Ostrea edulis (L.) based on AFLP and microsatellite markers
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),...
Tipo: Text
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf

Anti-predator response of Haliotis tuberculata is modified after only one generation of domestication
Provedor de dados: 5
Autores: Roussel, Sabine; Bisch, T; Lachambre, S; Boudry, Pierre; Gervois, Jl; Lambert, Christophe; Huchette, S; Day, R.
Domestication of Haliotis tuberculata has only recently begun. During the process, we expect that behavioural and physiological traits may evolve to become more adapted to their captive environment. These modifications may result from intentional selection of production traits or unconscious and unintentional selection due to conditions experienced in the farm environment. To study this process at the earliest stage, the progeny of 3 different broodstocks obtained from wild parents, selected farmed abalone and randomly sampled farmed abalone, were studied. After rearing for 16 mo in separate tanks, offspring from the 3 progenies were placed together in sea cages at the same density. After 3 yr, behavioural traits were studied, and the immune status after a...
Tipo: Text
Palavras-chave: Predation; Hiding behaviour; Haliotis tuberculata; Domestication; Selection; Captive; Abalone.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59841/62989.pdf

Transcriptome based SNP discovery and validation for parentage assignment in hatchery progeny of the European abalone Haliotis tuberculata
Provedor de dados: 5
Autores: Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre.
Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by...
Tipo: Text
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf

Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes
Provedor de dados: 5
Autores: Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre.
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials...
Tipo: Text
Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf

Point sur le programme huître plate - Complément au compte-rendu de réunion REGEMO sur l'Amélioration Génétique de l'huître plate du 11 mars 1998
Provedor de dados: 5
Autores: Goyard, Emmanuel; Lapegue, Sylvie; Gerard, Andre; Boudry, Pierre.
Ce document vient compéter le rapport REGEMO sur l'huître plate. Une première version visait essentiellement à faciliter le transfert de responsabilité du programme en cours. Celle-ci, épurée des aspects pratiques purement internes, se propose de faire le point sur les familles produites en 1998, et la continuité du programme d'amélioration.
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00049/15980/13422.pdf

Gill Development and Its Functional and Evolutionary Implications in the Blue Mussel Mytilus edulis (Bivalvia: Mytilidae)
Provedor de dados: 5
Autores: Cannuel, Rozenn; Beninger, Peter G.; Mccombie, Helen; Boudry, Pierre.
Study of gill development in bivalve larvae and postlarvae provides information on the evolution of this organ and feeding mechanisms of early stages. Scanning electron microscopy was used to document the development of the filibranch homorhabdic gill in hatchery-reared larval, postlarval, and juvenile Mytilus edulis. Four key stages were identified during gill development: (1) transfer of the particle collection function from velum to gill at metamorphosis, with subsequent elongation of the gill filaments to form a gill basket, with complete frontal ciliation; (2) reflection of the inner demibranchs, and transition to a V-shaped gill; (3) delayed development of the outer demibranchs, occuring simultaneously along the gill axis, with transition to the...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6935.pdf

Génomique de l'adaptation de l'huître creuse, Crassostrea gigas, dans le contexte de sa récente expansion géographique en Europe
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Originaire du nord de l’Asie, l'huître creuse, Crassostrea gigas, a été introduite et transférée, principalement pour l’aquaculture, dans plusieurs pays européens depuis le début des années 1970. Le caractère invasif de cette espèce a été observé au cours de la dernière décennie dans plusieurs pays et elle a même été considérée comme une espèce nuisible dans certaines régions, telles que la mer de Wadden. Dans le cadre du projet HiFlo, soutenu par l’ANR, nous avons pour objectif d'identifier les caractéristiques d'une telle espèce florissante: son succès peut-il être expliqué par le hasard des opportunités (expansion de leur niche écologique) ou est-il lié à des processus évolutifs, ce qui impliquerait une adaptation génétique de l'espèce à de nouvelles...
Tipo: Text
Palavras-chave: Huîtres; Crassostrea gigas; Génétique; Génétique des populations.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12552/9418.pdf

Evidence of response to unintentional selection for faster development and inbreeding depression in Crassostrea gigas larvae
Provedor de dados: 5
Autores: Taris, Nicolas; Batista, F; Boudry, Pierre.
Underlying consequences of domestication and artificial selection still remain largely unexplored in most aquacultured species. For species with a two phase life cycle, including the Pacific oyster Crassostrea gigas, most genetic studies have focused on the post-metamorphosis juvenile and adult stages, but relatively few considered the larval stage. To assess the consequence of hatchery practices on larval characters, especially growth, we performed a phenotypic study on larval progenies derived from crosses between Pacific oysters from natural beds and farmed Pacific oysters selected for desirable production traits such as rapid growth, for over seven generations. A set of three microsatellite loci was used to compare the genetic variability between the...
Tipo: Text
Palavras-chave: Crassostrea gigas; Inbreeding depression; Selection; Domestication; Larval stage.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3848.pdf

Comparative histological study of gametogenesis in diploid and triploid pacific oysters (Crassostrea gigas) reared in an estuarine farming site in France during the 2003 heatwave
Provedor de dados: 5
Autores: Normand, Julien; Le Pennec, Marcel; Boudry, Pierre.
We compared the temporal dynamics of gametogenesis in diploid and triploid Pacific oysters (Crassostrea gigas) using histology. Oysters were reared in an estuarine farming site in Brittany over 3 years and their gametogenesis was monitored over the exceptionally hot summer of 2003. Both diploids and triploids showed active gametogenesis, but a high proportion of triploids remained at early stages of gonad development. Gametogenesis of triploids was characterized by the simultaneous occurrence of gonias and immature cytes together with mature gametes rather than overall retardation, though some triploids showed complete gonad maturation. Evidence of spawning was seen in both groups, and the quantity of germinal products emitted appeared to be similar in...
Tipo: Text
Palavras-chave: Triploidy; Crassostrea gigas; Oyster; Gametogenesis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4152.pdf

Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text
Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00341/45195/44592.pdf

AFLP-based genetic linkage maps of the blue mussel (Mytilus edulis)
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Hecquet, Celine; Boudry, Pierre; Beaumont, A.
We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the male parent (type 1:1) and 154 through both parents (type 3:1). Chi-square goodness-of-fit tests revealed that 71% and 73% of type 1:1 and 3:1 markers respectively segregated according to Mendelian inheritance. Sex-specific linkage maps were built with mapmaker 3.0 software. The female framework map consisted of 121 markers ordered into 14 linkage groups, spanning...
Tipo: Text
Palavras-chave: Mytilus edulis; Genetic linkage map; Blue mussel; AFLP.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2830.pdf

Genetical basis of the plasticity of resource allocation in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Ernande, Bruno; Haure, Joel; Degremont, Lionel; Bedier, Edouard; Boudry, Pierre.
Phenotypic and genetic correlations between fitness-related life-history traits - such as survival, growth and reproductive allocation - have to be considered to better understand selective processes, both in the wild and in breeding programs. Quantitative genetic experiments, based on nested half-sib mating designs, were perfomed in the Pacific oyster C. gigas (1) to estimate genetic variance and the response to selection and (2) to document phenotypic and genetic trade-offs between life history traits. Physiological trade-offs, i.e. plastcity of resource allocation, were shown to vary among genotypes. Genetic polymorphism was primarily observed for the the plasticity of reproductive effort. In addition, the degree of plasticity in reproductive effort...
Tipo: Text
Palavras-chave: Genetic polymorphism; Genetic; Plasticity; Genetical basis; Crassostrea gigas; Pacific oysters.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-3443.pdf

Oyster population genetics : understanding natural populations and tracing introductions
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boudry, Pierre.
Oysters are among the most familiar of all marine invertebrate taxa. However our knowledge of oyster phylogeny and systematics is fragmentary. This is principally due to the plastic growth patterns of these animals, which result in a wide range of overlapping, ecophenotypic variants that greatly reduce the value of analysis based on shell morphology. Besides that, many intentional or accidental anthropogenic transfers have emphasised this situation. In several cases of misclassification or misidentification of oysters, DNA molecular data, have provided valuable new insights on the tracing of introductions or more generally on the phylogeographic relationships between oyster species. We will illustrate this issue with recent case studies from our lab on...
Tipo: Text
Palavras-chave: Genetic; Ostreola stentina; Crassostrea honkongensis; Crassostrea gigas; Crassostrea angulata; Oysters; Population genetics.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3475.pdf

New insights about the introduction of the Portuguese oyster, Crassostrea angulata, into the North East Atlantic from Asia based on a highly polymorphic mitochondrial region
Provedor de dados: 5
Autores: Grade, Ana; Chairi, Hicham; Lallias, Delphine; Power, Deborah M.; Ruano, Francisco; Leitao, Alexandra; Drago, Teresa; King, Jonathan W.; Boudry, Pierre; Batista, Frederico M.
It is commonly presumed that the Portuguese oyster Crassostrea angulata was introduced into the North East (NE) Atlantic from Asia. The analysis of the nucleotide sequence of a highly polymorphic non-coding mitochondrial region (major noncoding region - MNR) of C. angulata samples collected in Europe (Portugal), Africa (Morocco) and Asia (Shantou and Taiwan) provided new insight into the introduction of this species into the NE Atlantic. Sixty haplotypes and a nucleotide diversity of 0.0077 were observed in 130 analyzed sequences. Higher nucleotide diversity levels were observed in NE Atlantic sites than in Asian sites and significant genetic differentiation was found between the two. Our results suggest that C. angulata might have been introduced to the...
Tipo: Text
Palavras-chave: Crassostrea angulata; Phylogeography; Genetic signature; Biological invasions.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00371/48200/48316.pdf

Chromosome loss in bi-parental progenies of tetraploid Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre.
Alterations of chromosome number have been observed in the somatic tissue of Crassostrea gigas diploids and artificial polyploids. Tetraploid oysters with abnormal chromosome numbers in some or all of their tissue are considered undesirable as parents either for triploids (produced via a cross with diploids and of aquacultural interest) or for tetraploid breeding. Aneuploid tetraploid oysters may confer the tendency to lose chromosomes and to revert to lower ploidy levels to their offspring. More directly, their offspring could have a lower ploidy because of potential links between somatic and gametic chromosome loss. The present study evaluated the phenomenon in six bi-parental tetraploid families bred from parents of differing somatic ploidy quality. The...
Tipo: Text
Palavras-chave: Crassostrea gigas; Mitosis; Chromosome; Cytogenetics; Aneuploid; Polyptoid; Tetraploid; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-633.pdf

Effects of hydrodynamic factors on     Pecten maximus     larval development
Provedor de dados: 5
Autores: Holbach, Marine; Robert, Rene; Miner, Philippe; Mingant, Christian; Boudry, Pierre; Tremblay, Rejean.
Hatchery production of great scallop, Pecten maximus, remains unpredictable, notably due to poor larval survival. Large-scale flow-through systems up to 3500 L have been developed to avoid the use of antibiotics in static systems. Alternatively, small-scale flow-through systems have been successfully applied for oysters but they proved to be unsuitable to rear scallop larvae. By focusing on physical factors presumed to limit P. maximus larval development, this study aimed to optimize great scallop larvae rearing parameters under controlled conditions. First, the influence of aeration on larval performances, energetic metabolism and antioxidant defences were studied both in static and flow-through systems. Aeration depressed larval food intake, regardless...
Tipo: Text
Palavras-chave: Aeration; Flow-through; Larval physiology; Pecten maximus.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00384/49508/51151.pdf

Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet
Provedor de dados: 5
Autores: Geay, Florian; Culi, E. Santigosa I.; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y.; Corcos, L.; Bodin, Nathalie; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol...
Tipo: Text
Palavras-chave: Sea bass (Dicentrarchus labrax); FADS2 expression and activity; HUFA biosynthesis.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00008/11929/8635.pdf

Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - First Progress Report 1st January-31st December 1996 - Contract n° FAIR 95-421 Commission of the European Communities
Provedor de dados: 5
Autores: Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkinns, N. P.; Zouros, Eleftherios; Bonhomme, Francois.
The EEC project "Genephys 1996-2000", initiated by the IFREMER laboratory GAP (Génetique, Aquaculture et Pathologie, La Tremblade and CREMA (Centre de Recherche en Ecologie Marine et Aquaculture, l'Houmeau), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are: 1- To express the maximum variability of growth, both at the larval stage and at the adult stage, in an oyster population originating from parents of various origins. 2- To check the persistence over the time, of growth performance and related physiological functions. 3-...
Tipo: Text
Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00050/16149/13632.pdf

Comparative analysis of oxygen consumption rates between cupped oyster spat of Crassostrea gigas of French, Japanese, Spanish and Taiwanese origins
Provedor de dados: 5
Autores: Goulletquer, Philippe; Wolowicz, Maciej; Latala, Adam; Geairon, Philippe; Huvet, Arnaud; Boudry, Pierre.
Respiration rates of various geographical strains of Crassostrea gigas were compared to assess the respiratory expenditure as a physiological indicator of catabolism (cost of maintenance). Parental oysters, sampled in France (Marennes-Oleron), Japan (Hiroshima), Taiwan (Tunkang) and Spain (Cadix), were differentiated by both their geographic origin and by mitochondrial DNA markers, allowing the distinction between the two closely related taxa Crassostrea gigas and Crassostrea angulata. After reproduction of these parental oysters, respiration rates of spat of each strain, reared under common controlled conditions, were individually estimated at 20 degrees C by using a volumetric microrespirometer. Our results demonstrated that physiological variability...
Tipo: Text
Palavras-chave: Pacific Ocean; NE Atlantic; Oyster strains; Hybrids; Crassostrea angulata; Crassostrea gigas; Metabolism; Respiration rates; Océan Pacifique; Atlantique NE; Souches génétiques; Hybrides; Crassostrea angulata; Crassostrea gigas; Métabolisme; Taux de respiration.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-859.pdf

Bases génétiques et plasticité de la croissance et de la survie chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis.
Growth and survival are the two most interesting characters for oyster farming as they determine the biomass produced in the farms (growth x survival). Thus, a selection program aiming at improving these two characters could be of a great help. However, the oyster, as a sessile organism, is influenced by environmental variability (spatial and temporal) either natural or caused by the used oyster culture methods. Submitted to this variability, oysters must have evolved so as to adapt (short term) to this environmental variability.
Tipo: Text
Palavras-chave: Crassostrea gigas; Huîtres; Croissance; Plasticité; Génétique.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3279.pdf

Genetic improvement of hatchery propagated bivalve stocks : prospects and constraints
Provedor de dados: 5
Autores: Boudry, Pierre.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the production of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or other traits, could be obtained in diploids. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. In the U.S.A., Australia and New Zealand, family-based selective breeding programs have recently been initiated to improve growth, disease tolerance and yield.  In Europe, where both natural and...
Tipo: Text
Palavras-chave: Microsatellite markers; Crassostrea gigas; Selective breeding; Triploid; Pacific oyster; Genetic.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3451.pdf

Etude des processus de dérive et de sélection liés aux pratiques d'élevage en écloserie d'huître creuse
Provedor de dados: 5
Autores: Boudry, Pierre.
Genetic consequences of production of Pacific oyster larval in hatchery: drift and selective pressures related to rearing practices. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors were examined: the effects of discarding the smallest larvae (i.e. culling) and temperature effects. A mixed-family approach was used in order to infer the genetic composition of the larval population. The results show that high polymorphic microsatellite-based family assignment is a powerful tool for the study of bivalve larvae genetics. Culling by selective sieving is an advantageous practice at a phenotypic scale, but also represents a substantial risk for diversity loss if parentage assignment is not introduced as a...
Tipo: Text
Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-1459.pdf

Taxonomie de l'huître naine Ostreola stentina
Provedor de dados: 5
Autores: Lapegue, Sylvie; Ben Salah, Inès; Batista, Frederico; Heurtebise, Serge; Neifar, Lassad; Boudry, Pierre.
Despite the economic importance of numerous oyster species throughout the world, our knowledge of their systematics and phylogeography remains fragmentary. In Europe, the indigenous species, Ostrea edulis, suffered overexploitation as well as two successive parasitosis over the last century. However, this species is still cultivated in Europe, and especially in Spain and France. Its distribution area stretches from the Atlantic coasts of Europe to the north of Africa as well as around the Mediterranean and Black Sea. Its Mediterranean and South Atlantic distribution area is shared with another indigenous species, the dwarf oyster, Ostreola stentina. Its very small adult size (a few centimetres) makes its culture pointless from a commercial point of view....
Tipo: Text
Palavras-chave: Ostreola stentina; Oyster; Taxonomy; Ostreola stentina; Huître; Taxonomie.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3364.pdf

FADS2: A potential limiting step in HUFA metabolic pathway of European sea bass (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Tipo: Text
Ano: 2010

Assortative fertilization and selection at larval stage in the mussels Mytilus edulis and M-galloprovincialis
Provedor de dados: 5
Autores: Bierne, Nicolas; David, Pierre; Boudry, Pierre; Bonhomme, F.
Assortative mating (prezygotic isolation) and reduced hybrid fitness (postzygotic isolation) are typically invoked to explain the stability of hybrid zones. In the tension zone model. these factors work in opposition to migration, which promotes genetic homogeneity. Many marine animals migrate over long distances through a planktonic larval stage. Therefore. strong reproductive isolation is needed to maintain stable marine hybrid zones. However, surprisingly little is known about mating preferences and hybrid fitness in marine organisms. Smooth-shelled mussels (Mytilus spp.) form a well-known species complex, with hybridization over extensive areas such as the contact zone of M. edulis and M. galloprovincialis around European Atlantic coasts. This paper...
Tipo: Text
Palavras-chave: Preferential fertilization; Mytilus galloprovincialis; Mytilus edulis; Hybrid viability; Heterosis; Gamete competition.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2781.pdf

Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie.
We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage...
Tipo: Text
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf

The Kinome of Pacific Oyster Crassostrea gigas, Its Expression during Development and in Response to Environmental Factors
Provedor de dados: 5
Autores: Epelboin, Yanouk; Quintric, Laure; Guevelou, Eric; Boudry, Pierre; Pichereau, Vianney; Corporeau, Charlotte.
Oysters play an important role in estuarine and coastal marine habitats, where the majority of humans live. In these ecosystems, environmental degradation is substantial, and oysters must cope with highly dynamic and stressful environmental constraints during their lives in the intertidal zone. The availability of the genome sequence of the Pacific oyster Crassostrea gigas represents a unique opportunity for a comprehensive assessment of the signal transduction pathways that the species has developed to deal with this unique habitat. We performed an in silico analysis to identify, annotate and classify protein kinases in C. gigas, according to their kinase domain taxonomy classification, and compared with kinome already described in other animal species....
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00340/45069/44494.pdf

Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas
Provedor de dados: 5
Autores: Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre.
Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combination with OsHV-1 DNA quantification by standard real-time PCR and total vibrio population levels in oyster tissues. To examine the effect of seawater temperature on disease transmission and related mortality of oysters, cohabitation experiments were conducted between healthy naïve oysters and oysters previously exposed to field conditions in areas where mortalities were occurring. Oysters initially maintained in controlled conditions (free of mortality and negative...
Tipo: Text
Palavras-chave: Juvenile oyster; Pathology; Temperature; Survival; Vibrios; Ostreid herpesvirus 1.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00148/25891/23912.pdf

Exploitation par la pêche de coquillages d’écloserie en rade de Brest : quels acteurs pour quelles perspectives ?
Provedor de dados: 5
Autores: Danto, Anatole; Boudry, Pierre; Ragueneau, Olivier; Maze, Camille.
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00375/48577/48899.pdf

Recent advances in EST sequencing in Crassostrea gigas: towards the sequencing of the Pacific oyster genome
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud; Lapegue, Sylvie; Sauvage, Christopher; Tanguy, A.; Moraga, D.; Goostrey, A.; Prunet, P.; Boulo, Viviane; Gueguen, Y.; Moal, Jeanne; Fleury, Elodie; Samain, Jean-francois; Mathieu, M.; Favrel, P.
Rationale for EST and genome sequencing of the Pacific oyster: • its membership of the Lophotrochozoa, an little studied branch of the Eukaryotes, • its worldwide aquacultural importance (4.2 million metric tons, worth 3.5 billion US dollars), • its high fecundity, with concomitantly high DNA polymorphism, • its use as a biosensor of coastal pollution. In 2004, an international community of biologists, the Oyster Genome Consortium (OGC) led by D. Hedgecock (University of Southern California) and uniting 70 participants from 10 countries, first presented the Pacific oyster Crassostrea gigas as a genome-sequencing candidate to Joint Genome Institute (JGI, USA) (Hedgecock et al., 2005). Besides this project (that remains to be accepted), several EST...
Tipo: Text
Palavras-chave: Characteristics; Genome size; Genome; Crassostrea gigas; Oyster; Genetic.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3414.pdf

The taxonomic status and origin of the Portuguese oyster Crassostea angulata (Lamark, 1819)
Provedor de dados: 5
Autores: Batista, Frederico; Leitao, Alexandra; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre.
The taxonomic status of the Portuguese oyster Crassostrea angulata and the Pacific oyster Crassostrea gigas has often been a matter of controversy. Based on larval shell morphology, experimental hybridisation and electrophoretic studies of enzyme polymorphism several authors have considered these two species as being synonymous. During the recent years, several genetic studies based on mitochondrial DNA and microsatellites data have provided evidences that the two taxa are genetically distinct although closely related. Furthermore, karyotype analyses have also highlighted the close genetic similarity of these taxa in comparison with other cupped oyster species. However, a comparative analysis of restriction enzymes ideograms revealed differences between...
Tipo: Text
Palavras-chave: Geographical origin; Phylogenetic; Genetic; Taxonomic status; Crassostrea angulata; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3433.pdf

Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Bigot, Laetitia; Zatylny-gaudin, Celine; Rodet, Franck; Bernay, Benoit; Boudry, Pierre; Favrel, Pascal.
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the...
Tipo: Text
Palavras-chave: Mollusk; Bivalve; Oyster; Neuroendocrinology; Reproduction; GnRH.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00079/19003/17477.pdf

Apport d'un programme de génétique à une filière de production aquacole : l'exemple de l'ostréiculture
Provedor de dados: 5
Autores: Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre.
Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between...
Tipo: Text
Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf

Etude des ressources génétiques chez les huîtres creuses
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Lam, Katherine; Mccombie, Helen; Leitao, Alexandra; Gerard, Andre.
L'étude de la variabilité génétique de l'huître creuse Crassostrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 'ro de la production mondiale d'huîtres. Introduite en France au début des années 1970 pour remplacer l'huître portugaise Crassostrea angulata, c'est aujourd'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n'a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la...
Tipo: Text
Palavras-chave: Génétique; Huîtres creuses; Crassostrea gigas; Crassostrea gasar; Variabilité génétique.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31008/29411.pdf

Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions
Provedor de dados: 5
Autores: Delaporte, Maryse; Soudant, P; Lambert, C; Jegaden, M; Moal, Jeanne; Pouvreau, Stephane; Degremont, Lionel; Boudry, Pierre; Samain, Jean-francois.
Within the framework of a national scientific program named "MORtalites ESTivales de l'huitre creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassostrea gigas. As part of the MOREST project, the second generation of three resistant families and two susceptible families were chosen and pooled into two respective groups: "R" and "S". These two groups of oysters were conditioned for 6 months on two food levels (4% and 12% of oyster soft-tissue dry weight in algal dry weight per day) with a temperature gradient that mimicked the Marennes-Oleron natural cycle during the oyster reproductive period. Oyster mortality remained low for the first two...
Tipo: Text
Palavras-chave: Summer mortality; Reproduction; Reactive oxygen species ROS; Hemocyte parameters; Genetic selection; Crassostrea gigas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3578.pdf

Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species
Provedor de dados: 5
Autores: Huvet, Arnaud; Boudry, Pierre; Ohresser, M; Delsert, Claude; Bonhomme, F.
Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Cloning System, CA). White colonies (11,500) were screened by hybridisation with double-stranded DNA probes containing dinucleotide repeats. Poly(dAdC), poly(dAdG), poly(dAdT) (Pharmacia Biotech, Sweden) were mixed at equimolar ratio and radiolabelled by [&amp;#61537;-32P] alpha dATP and dCTP using a random priming labelling kit (Life Technologies, Germany). Filter...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oyster; Microsatellites.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-703.pdf

Reproductive effort of Pacific oysters: A trait associated with susceptibility to summer mortality
Provedor de dados: 5
Autores: Huvet, Arnaud; Normand, Julien; Fleury, Elodie; Quillien, Virgile; Fabioux, Caroline; Boudry, Pierre.
Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment and their pathogens. The physiological status of an oyster, especially its reproductive status, is suspected to play a significant role in the outcome of this interaction. As genetic variability exists for susceptibility to summer mortality, resistant (R) and susceptible (S) oyster lines were produced using a divergent selection scheme. The present paper reports a histological study on gonad area, which is representative of reproductive effort, in randomly chosen five R and five S oyster lines. The R lines showed a significantly lower gonad area than the S lines (P&lt;0.001). with an estimated mean difference of 12.5%, whereas,...
Tipo: Text
Palavras-chave: Bivalve mollusc; Crassostrea gigas; Reproduction; Selected lines; Summer mortality.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11766/8502.pdf

Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (Poster)
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Early-life history of oysters, like in most marine bivalves, is characterized by high fecundity and low survival rate. In hatcheries, which are ecoming more and more important for the production of juveniles for aquaculture production, rearing conditions are optimised (density, feeding, temperature...), and contrast with those observed in the wild. Additionally, slow growing larvae are culled by sieving.
Tipo: Text
Palavras-chave: Crassostrea gigas; Juvenile; Genetic variability; Selection; Genetic; Hatcheries; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3444.pdf

Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Corporeau, Charlotte; Vanderplancke, Gwenaelle; Boulais, Myrina; Suquet, Marc; Quere, Claudie; Boudry, Pierre; Huvet, Arnaud; Madec, Stephanie.
We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and...
Tipo: Text
Palavras-chave: Marine bivalves; Crassostrea gigas; Oocyte quality; Proteomics.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20925/18534.pdf

Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Rapport année 1995
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Heral, Maurice; Collet, Bertrand; Bougrier, Serge; Geffard, Olivier; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre.
Le programme de recherche présenté ci-dessous est le fruit d'une réflexion conjointe entre généticiens et physiologistes afin de mettre sur pied un ensemble d'expériences permettant d'étudier les bases génétiques de la variabilité des caractères impliqués dans la croissance chez l'huître creuse, Crassostrea gigas.
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Génétique des populations; Croissance; Crassostrea gigas.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00044/15518/12905.pdf

Genetic variation and trade-offs for reproduction ans survival in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Boudry, Pierre; Degremont, Lionel; Bedier, Edouard; Pouvreau, Stephane; Normand, Julien; Ernande, Bruno.
To date, the most significant method to genetically reduce reproductive effort in the Pacific oyster (Crassostrea gigas) has been through the production of triploids, especially since the development of tetraploid lines allowing the breeding of 'natural' triploids. Gametogenesis of triploid oysters is strongly reduced compared with diploids, although they are not fully sterile and can produce viable gametes and some progenies when crossed with diploids. Reduced reproductive allocation and higher heterozygocity are commonly proposed as the main reasons why triploids often present superior yield compared with diploids. Temperature and food availability are known to favour gametogenesis in triploids, but the existence of genetic variation for this trait...
Tipo: Text
Palavras-chave: Selective breeding; Reproduction; Genetic variation; Crassostrea gigas; Pacific oyster; Genetic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3445.pdf

The Proposed Dropping of the Genus Crassostrea for All Pacific Cupped Oysters and Its Replacement by a New Genus Magallana: A Dissenting View
Provedor de dados: 5
Autores: Bayne, B. L.; Ahrens, M.; Allen, S. K.; D'Auriac, M. Angles; Backeljau, T.; Beninger, P.; Bohn, R.; Boudry, Pierre; Davis, J.; Green, T.; Guo, X.; Hedgecock, D.; Ibarra, A.; Kingsley-smit, P.; Krause, M.; Langdon, C.; Lapegue, Sylvie; Li, C.; Manahan, D.; Mann, R.; Perez-paralle, L.; Powell, E. N.; Rawson, P. D.; Speiser, D.; Sanchez, J. -l.; Shumway, S.; Wang, H.
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00418/52944/53886.pdf

Le phénomène de l'anauploïdie et sa relation avec la croissance chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Leitao, Alexandra; Boudry, Pierre; Mccombie, Helen; Lapegue, Sylvie; Gerard, Andre; Thiriot-quievreux, Catherine.
Cytogenetic abnormalities have often been observed on bivalves on meiosis or mitosis. This is a review of various observations of somatic aneuploidy conducted on different populations of Crassastrea gigas.
Tipo: Text
Palavras-chave: Croissance; Aneuploïdie; Huîtres; Génétique; Crassostrea gigas; Cytogénétique.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3280.pdf

QTL mapping for resistance to bonamiosis in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2...
Tipo: Text
Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf

Amylase polymorphism affects growth in the cupped oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Samain, Jean-francois; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Van Wormhoudt, A.
The better understanding of physiological and environmental factors that determine optimal food conversion efficiencies is of major interest for the cupped oyster Crassostrea gigas for which the strong increase of aquaculture has been correlated in France with a decrease in productivity due to competition between aquatic species for limited food supplies at grow-out sites. To investigate the non-neutrality of the polymorphism of amylase, a key enzyme for carbohydrate assimilation, in oyster physiology, we report in the present paper the growth and survival measurement of amylase genotypes within five bi-parental C. gigas families, bred to be polymorph at the two PCR-RFLP amylase markers, and reared over one year under standard culture conditions in two...
Tipo: Text
Palavras-chave: Amylase markers; Growth; Amylase Polymorphism; Genetic; Oysters; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3311.pdf

ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas )
Provedor de dados: 5
Autores: Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc.
Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability...
Tipo: Text
Palavras-chave: Spermatozoa; Quality; Motility; Viability; ATP content; ROS production.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00386/49769/50309.pdf

Population genomics shed light on the demographic and adaptive histories of European invasion in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Crassostrea gigas originated from the Pacific coast of Asia, but was introduced into several European countries in the early 1970s. Natural populations have now spread across the length of the western seaboard of Europe. To elucidate the demographic and selective processes at play during this rapid expansion, genome-scan analysis was performed on different populations. High diversities and low differentiation were observed overall, but significant genetic differentiation was found among newly established populations and between the newly established northern group and a nearly panmictic group composed of southern European populations and a population from Japan. Loss of genetic diversity was also seen in the north, likely caused by founder events during...
Tipo: Text
Palavras-chave: AFLPs; Crassostrea gigas; Genome scan; Invasive species; Microsatellites; SNPs.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00149/25977/24070.pdf

Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids
Provedor de dados: 5
Autores: Batista, Frederico M.; Lopez-sanmartin, Monserrat; Boudry, Pierre; Navas, Jose I.; Ruano, Francisco; Renault, Tristan; Fonseca, Vera G.; Leitao, Alexandra.
Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid...
Tipo: Text
Palavras-chave: Aneuploidy; Ostreid herpesvirus 1; Oyster; Crassostrea.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00215/32581/35759.pdf

Strategies for the retention of high genetic variability in European flat oyster (Ostrea edulis) restoration programmes
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Lapegue, Sylvie; King, Jon W.; Beaumont, Andy R.
The native European flat oyster Ostrea edulis is listed in the OSPAR Convention for the Protection of the Marine Environment of the North-East Atlantic (species and habitat protection) and in the UK Biodiversity Action Plan. Once extremely abundant in the 19th century, European stocks of O. edulis have declined during the 20th century to rare, small, localised populations due to overexploitation, habitat degradation and, most recently, the parasitic disease bonamiosis. Selective breeding programmes for resistance to bonamiosis have been initiated in France and Ireland. High genetic diversity and bonamiosis-resistance would be important features of any sustainable restoration programmes for O. edulis. Oysters were sampled across Europe from four hatchery...
Tipo: Text
Palavras-chave: Restoration programme; Ostrea edulis; Genetic variation; Pedigree reconstruction.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00001/11259/11375.pdf

Microsatellite analysis of 6-hour-old embryos reveals no preferential intraspecific fertilization between cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Huvet, Arnaud; Balabaud, K; Bierne, Nicolas; Boudry, Pierre.
Experimental examination of reproductive isolation is the first step in understanding li,hybridization processes. Here, we studied preferential fertilization between 2 cupped oyster taxa, Crassostrea angulata and Crassostrea gigas, as a potential prezygotic reproductive isolation. Early examination of sperm competition is now possible by molecular analysis of oyster embryos. This avoids the confounding effect of differential mortality during the larval stage. Six hundred embryos were sampled from 2 crosses. Three microsatellite loci were enough to determine without ambiguity the taxa of contributing sires of embryos. No evidence of preferential fertilization between gametes from the same taxa was shown. A significantly higher contribution of the C. gigas...
Tipo: Text
Palavras-chave: Paternal contribution; Microsatellites; In vitro crossings; Hybridization; Crassostrea angulata; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-436.pdf

Exploring local reproductive differentiation of Pacific oyster population recently settled in northern Europe by transcriptomic approach
Provedor de dados: 5
Autores: Sussarellu, Rossana; Huvet, Arnaud; Lelong, Christophe; Pernet, Fabrice; Quillen, Virgile; Lapegue, Sylvie; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Originating from north-eastern Asia, the Pacific oyster Crassostrea gigas has been introduced, mainly for aquaculture purpose, into a large number of countries. Although highly variable, its invasive nature has been reported in an increasing number of coastal areas, notably. Northern Europe. We set up a common garden experiment based on the comparison of progenies of Pacific oysters sampled in France, where populations settled since the introduction of the species in the 70’s, and Denmark, where established populations were observed since the beginning of 90s. Pure and hybrid progenies, resulting from crosses within and between adults sampled in both populations, were studied for their sex-ratio, condition index, transcriptomics, and biochemical parameters
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27815/26008.pdf

Influence of temperature on disease transmission associated with ostreid herpes virus OSHV-1 µVAR in relation to survival of juvenile Crassostrea gigas
Provedor de dados: 5
Autores: Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre; Alunno-bruscia, Marianne.
Since 2008, mass mortalities of 1-yr-old oysters Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have occurred along all coasts of France. These mortality events represent the most serious crisis for the French oyster industry since the introduction of C. gigas in the early 1970s. Temperature is commonly one of the major triggering factors of disease epizootics, notably for aquatic species. In France, OsHV-1 is generally detected in dying oysters when seawater temperature is higher than 16° C. The influence of temperature on OsHV-1 detection and expression was also demonstrated for C. gigas larvae and suspected for juveniles. The present study aims to characterize the effect of temperature on disease transmission and survival related...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00151/26259/24343.pdf

Relative importance of family, site, and field placement timing on survival, growth, and yield of hatchery-produced Pacific oyster spat (Crassostrea gigas)
Provedor de dados: 5
Autores: Degremont, Lionel; Bedier, Edouard; Soletchnik, Patrick; Ropert, Michel; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre.
Summer mortality has been reported in the Pacific oyster, Crassostrea gigas, for many years in different parts of the world. The causes of this phenomenon are complex. The multidisciplinary program "MOREST", coordinated by IFREMER, was initiated to understand the causes of summer mortality of Crassostrea gigas juveniles in France and to reduce its impact on oyster production. Within this program, three successive groups of bi-parental families were bred in a hatchery in 2001 and placed in the field during summer in three sites (Ronce, Riviere d'Auray, and Baie des Veys). This paper reports the relative importance of family, site and field placement timing for three characters of major importance for oyster production: survival, growth, and yield. At the...
Tipo: Text
Palavras-chave: Juvenile; Crassostrea gigas; Oysters; Yield; Growth; Survival.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1693.pdf

Gametogenesis in the Pacific Oyster Crassostrea gigas: A Microarrays-Based Analysis Identifies Sex and Stage Specific Genes
Provedor de dados: 5
Autores: Dheilly, Nolwenn; Lelong, Christophe; Huvet, Arnaud; Kellner, Kristell; Dubos, Marie-pierre; Riviere, Guillaume; Boudry, Pierre; Favrel, Pascal.
Background: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. Methodology/Principal Findings: Gene expression was...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19862/17505.pdf

Hybridization and morphological differences between the two closely related oyster taxa Crassostrea angulata and C. gigas
Provedor de dados: 5
Autores: Batista, Frederico; Fonseca, Vera; Ben-hamadou, Radhouan; Taris, Nicolas; Henriques, Maria; Boudry, Pierre.
Unlike in some crops, no large heterotic effects have been observed in most farmed animals. Interestingly, significant hybrid vigour for some traits has been reported in the Pacific oyster, Crassostrea gigas. Crossbreeding between closely related taxa can be seen as an efficient way to exploit the dominance component of the genetic variance of a trait. Factorial crosses between the Portuguese oyster C. angulata (from Sado estuary, Portugal) and C. gigas (from Seudre estuary, France) were done to evaluate the aquaculture potential of hybrids. Juveniles of the different progenies were reared in Ria Formosa (Portugal) under usual farming conditions. The genetic confirmation of all progenies was done using mitochondrial and nuclear markers in order to detect...
Tipo: Text
Palavras-chave: Hybridization; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3429.pdf

Analysis of genome-wide differentiation between native and introduced populations of the cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie.
The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end...
Tipo: Text
Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf

Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Several studies have suggested that significant genetic variability might exist for some early developmental traits in the Pacific oyster, C. gigas. However, most studies have examined only limited numbers of families.  More critically, these families were rarely replicated, leading to possible bias due to heterogeneous larval rearing conditions among families. To overcome these potential problems, we used a mixed-family approach with subsequent parentage analysis. A recently developed set of three multiplexed microsatellite markers was used to determine parentage in a complete factorial cross between 12 males and 4 females. Larvae were reared at two different temperatures (20 and 26°C) to provide a contrast resembling wild versus hatchery conditions....
Tipo: Text
Palavras-chave: Selection; Genetic variability; Microsatellites; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3440.pdf

Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae
Provedor de dados: 5
Autores: Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Tipo: Text
Palavras-chave: DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2916.pdf

Etude de faisabilité d’opérations de ‘repeuplement orienté’ dans deux sites français de captage de l’huître creuse (Crassostrea gigas)
Provedor de dados: 5
Autores: Bernard, Ismael; Lapegue, Sylvie; Dumas, Franck; Talarmain, Eric; Cornette, Florence; Degremont, Lionel; Chavanne, Herve; Pouvreau, Stephane; Auby, Isabelle; Maurer, Daniele; Plus, Martin; Le Moine, Olivier; Boudry, Pierre.
La forte augmentation des mortalités de juvéniles d’huître creuse depuis 2008 a relancé la problématique de l’approvisionnement en naissains de l’ostréiculture française. Dans ce contexte, cette étude a pour objectif d’étudier la faisabilité d’opérations de repeuplement en huîtres génétiquement plus résistantes, afin d’améliorer les performances de survie du naissain capté dans le milieu naturel. Cette étude s’est ainsi focalisée sur les deux bassins naisseurs historiques, Marennes-Oléron et Arcachon. Nous avons donc cherché à établir (1) une hiérarchie des bancs en fonction de l’expulsion de larves d’huître dans le bassin d’Arcachon, (2) une cartographie des liens en termes de connectivité entre les bancs des pertuis charentais et (3) la faisabilité du...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00083/19410/17013.pdf

Mitochondrial and Nuclear DNA Analysis of Genetic Heterogeneity Among Recruitment Cohorts of the European Flat Oyster Ostrea edulis
Provedor de dados: 5
Autores: Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Camara, Mark D.; Lapegue, Sylvie.
Marine species with high fecundity and high early mortality may also have high variance in reproductive success among individuals due to stochastic factors, making successful reproduction a "sweepstakes." In some cases, the impact is sufficient to reduce the effective number of breeders in wild populations. We tested two predictions of the sweepstakes reproductive success hypothesis in a French Atlantic population of the European flat oyster, Ostrea edulis, by evaluating (1) whether individuals belonging to temporally discrete recruitment cohorts within a single reproductive season displayed reduced genetic variation relative to the entire adult population, and (2) whether these temporal cohorts of recruits were genetically differentiated from each other....
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7399.pdf

Stock enhancement or sea ranching? Insights from monitoring the genetic diversity, relatedness and effective population size in a seeded great scallop population (Pecten maximus)
Provedor de dados: 5
Autores: Morvezen, R.; Boudry, Pierre; Laroche, J.; Charrier, G.
The mass release of hatchery-propagated stocks raises numerous questions concerning its efficiency in terms of local recruitment and effect on the genetic diversity of wild populations. A seeding program, consisting of mass release of hatchery-produced juveniles in the local naturally occurring population of great scallops (Pecten maximus L.), was initiated in the early 1980s in the Bay of Brest (France). The present study aims at evaluating whether this seeding program leads to actual population enhancement, with detectable effects on genetic diversity and effective population size, or consists of sea ranching with limited genetic consequences on the wild stock. To address this question, microsatellite-based genetic monitoring of three hatchery-born and...
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00344/45530/45105.pdf

Genetic characterisation of oyster populations along the north-eastern coast of Tunisia
Provedor de dados: 5
Autores: Dridi, S.; Romdhane, M. S.; Heurtebise, Serge; El Cafsi, M.; Boudry, Pierre; Lapegue, Sylvie.
The taxonomy of oysters has been traditionally based on characteristics of the shell. More recently, the analysis of protein and DNA polymorphism has provided a means to overcome difficulties in distinguishing the different species of oysters based solely on shell morphology. In order to identify oysters of the Tunisian north-east coast, we sequenced a 16S rRNA mitochondrial fragment from 68 oysters sampled from the Bizert Lagoon and the Gulf of Hammamet in northern Tunisia. Comparison of oyster 16S rRNA sequences available in GenBank showed the presence of both Ostreola stentina and Crassostrea gigas in our samples, which could not be detected on the basis of shell morphology only. These data confirmed that C. gigas, a non-native species, is now...
Tipo: Text
Palavras-chave: 16S rRNA; Crassostrea gigas; Genetics; Mediterranean Sea; Ostreola stentina; Polymorphism.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00000/11063/7820.pdf

Mortalités massives de l’Huître creuse: causes et perspectives
Provedor de dados: 5
Autores: Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Lagarde, Franck; Fiandrino, Annie; Huvet, Arnaud; Corporeau, Charlotte; Boudry, Pierre; Quere, Claudie; Degremont, Lionel; Pepin, Jean-francois; Saulnier, Denis; Boulet, Hélène; Keck, Nicolas.
The objectives of this study are to increase our understanding of the mass mortality phenomena in Pacific oysters Crassostrea gigas in the Thau lagoon and to examine the effect of cultivation practises. Mortality events in 2009 and 2010 always coincide with simple or dual infections involving primarily the Herpes virus OsHV-I and secondarily the bacteria Vibrio splendidus, when seawater temperature is between 17°C and 24°C. In the Thau lagoon, mortality starts early May and last until fall with an interruption period when seawater temperature is higher than 24°C in July and August. Mortality of oysters varies as a function of zootechnical life-history, hydrodynamics and cultivation practises. First, mortality depends on whether or not oysters have been...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00043/15404/12764.pdf

Genetic impact of the reproduction dynamics in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual...
Tipo: Text
Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huîtres creuses Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 97 RPC-R-54 "Génétique" - Rapport année 1997
Provedor de dados: 5
Autores: Boudry, Pierre; Lapegue, Sylvie; Goyard, Emmanuel; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1997 étaient les suivants : • Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. • Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant limitée).
Tipo: Text
Palavras-chave: Génétique; Génétique des Populations; Acclimatation; Hybridation; Marqueurs génétiques; Sélection génétique; Crassostrea gigas.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00044/15519/12906.pdf

A first genetic linkage map of the blue mussel Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Hecquet, Céline; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy.
The blue mussel, Mytilus edulis, is widely distributed in the northern hemisphere, from the White Sea to the Atlantic coast of southern France, and is an economically important species (1 million tons per year, FAO, 2002). At present however, little domestication has been achieved with bivalves, in contrast to agricultural crops, livestock and horticultural species like tomatoes, where selection and production is entirely controlled by man. For bivalves, despite their economic importance, no lines with selected characteristics have been taken to the production scale. There is therefore a need to make up for lost time. Our interest in genetic mapping sprang from some results showing a genetic component to some economically important traits like growth and...
Tipo: Text
Palavras-chave: QTLs; AFLP; Genetic; Mussel; Mytilus edulis; Genetic linkage map.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3465.pdf

Genetic structure of wild European populations of the invasive Pacific oyster Crassostrea gigas due to aquaculture practices
Provedor de dados: 5
Autores: Meistertzheim, Anne-leila; Arnaud-haond, Sophie; Boudry, Pierre; Thebault, Marie-therese.
As a result of aquaculture activities, Pacific oysters Crassostrea gigas (Thunberg, 1793) have invaded European coasts. Using seven microsatellites, we found virtually no genetic differentiation between natural populations throughout the European range (from the south of the Wadden Sea (the Netherlands) to the south of France) and French cultivated oysters. The genetic homogeneity of Pacific oyster samples appears to be the result of repeated transfers from same seed stocks made for aquaculture and, to a lesser extent, widespread dispersal due to specific biological traits of this species. The only genetic differentiation of Sylt population in the north of the Wadden Sea (Germany) suggests a stronger, persistent impact of ongoing supply of new genetic...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00119/23051/20924.pdf

Phylogeography of mangrove oysters from the Southern Atlantic ocean : Crassostrea gasar and Crassostre rhizophorae
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Thiriot-quievreux, Catherine; Heurtebise, Serge; Garcia, P.; Boudry, Pierre.
Mangrove oysters encompass several species and their taxomony is unclear. Among then, Crassostrea rhizophorae has been described along the Central and South American Atlantic coasts and Crassostrea gasar along African Atlantic coasts. Most taxonomic studies have so far been based on morphological criteria which are particularly susceptible to plasticity and new insight can be provided by molecular markers. The present work aims to provide information on the phylogeography of these two taxa.
Tipo: Text
Palavras-chave: Genetic; Phylogeography; Crassostrea rhizophorae; Crassostrea gasar; Mangrove oysters.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3496.pdf

Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe
Provedor de dados: 5
Autores: Huvet, Arnaud; Lapegue, Sylvie; Magoulas, A; Boudry, Pierre.
The respective status of the Portuguese oyster, Crassostrea angulata, and the Pacific oyster, Crassostrea gigas, has long been a matter of controversy. Morphological and physiological similarities, homogeneity of allozyme allelic frequencies between populations of the two taxa and the demonstration of hybridization lead most authors to suggest that they should be regrouped within the same species. The risk of introgression and the present expansion of C. gigas aquaculture in Europe raises the question of the need for preservation of C. angulata in Europe, as only a few populations remain. We studied European and Asian populations of C. gigas and C. angulata using microsatellite and mitochondrial DNA markers to estimate their genetic diversity and...
Tipo: Text
Palavras-chave: Phylogeography; Population genetics; Microsatellites; Crassostrea gigas; Crassostrea angulata.
Ano: 2000
URL: https://archimer.ifremer.fr/doc/2000/publication-707.pdf

Factors influencing disease-induced mortality of Pacific oysters Crassostrea gigas
Provedor de dados: 5
Autores: Petton, Bruno; Boudry, Pierre; Alunno-bruscia, Marianne; Pernet, Fabrice.
Mortalities of oyster Crassostrea gigas seed associated with ostreid herpesvirus OsHV-1 μVar have been observed in many oyster-producing countries since 2008. The present study, comprised of 4 complementary experiments, aimed to identify factors associated with disease-induced oyster mortality in order to propose mitigation strategies. Our first experiment compared survival of oysters from natural spatfall with others sampled from nurseries, after thermal elevation in the laboratory from &lt;14 to 21°C. A total of 60% of the tested wild seed batches (n = 51) were infected by OsHV-1, exhibited mortality and were able to transmit the disease to cohabited naïve oysters. Comparatively, only 1 out of the 32 tested batches sampled from nurseries presented...
Tipo: Text
Palavras-chave: Epidemiology; Risk analyses; Shellfish farming; Virus OsHV-1 mu Var.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00257/36777/35390.pdf

Reduced female gene flow in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Diaz Almela, F; Boudry, Pierre; Launey, Sophie; Bonhomme, Francois; Lapegue, Sylvie.
The geographical structure of 15 natural populations of the flat oyster (Ostrea edulis L.) was assessed by single-strand conformation polymorphism (SSCP) of a 313-base-pair (bp) fragment of the mitochondrial 12S-rRNA gene. Fourteen haplotypes were observed, with one being dominant in the Mediterranean samples and another one in the Atlantic populations. The geographically extreme populations sampled in Norway and the Black Sea appeared differentiated by exhibiting the dominance of a third group of haplotypes. The results were compared to available microsatellite data at five loci. The Atlantic/Mediterranean differentiation pattern was qualitatively the same with both types of markers, confirming an isolation-by-distance pattern. The average mitochondrial...
Tipo: Text
Palavras-chave: Ostrea edulis; Polymorphism; Flat oyster; Population; Gene.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-680.pdf

GigaTON: an extensive publicly searchable database providing a new reference transcriptome in the pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Riviere, Guillaume; Klopp, Christophe; Ibouniyamine, Nabihoudine; Huvet, Arnaud; Boudry, Pierre; Favrel, Pascal.
Background: The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture shellfish resources worldwide. Important efforts have been undertaken towards a better knowledge of its genome and transcriptome, which makes now C. gigas becoming a model organism among lophotrochozoans, the under-described sister clade of ecdysozoans within protostomes. These massive sequencing efforts offer the opportunity to assemble gene expression data and make such resource accessible and exploitable for the scientific community. Therefore, we undertook this assembly into an up-to-date publicly available transcriptome database: the GigaTON (Gigas TranscriptOme pipeliNe) database. Description: We assembled 2204 million sequences obtained from 114 publicly...
Tipo: Text
Palavras-chave: Oyster; Transcriptome; Development; Stress; Tissues; NGS.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00302/41321/40520.pdf

Infestation of the cupped oysters Crassostrea angulata, C. gigas and their first-generation hybrids by the copepod Myicola ostreae: differences in susceptibility and host response
Provedor de dados: 5
Autores: Batista, F. M.; Boudry, Pierre; Dos Santos, A.; Renault, Tristan; Ruano, F.
We Studied the prevalence and intensity of the parasitic copepod Myicola ostreae in 2 closely related oysters Crassostrea angulata and C. gigas and their F1 hybrids. The effects on host and host reaction were also analysed to better understand host-parasite relationships between copepods and bivalve molluscs. Full reciprocal crosses were carried out between C. angulata and C. gigas and the progenies were reared in the wild in Ria Formosa Lagoon (Portugal), allowing natural infestation by M. ostreae. Prevalence and intensity were significantly higher in C. angulata than in C. gigas. The parasite level of F1 hybrids was similar to C. angulata and significantly higher than in C. gigas. The results of our study support a hypothesis of dominantly inherited...
Tipo: Text
Palavras-chave: Encapsulation; Host reaction; Host parasite relationship; Dominance inherence; Parasitism; Hybridization; Copepods; Oysters.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6442.pdf

A comparative field study of growth, survival and reproduction of Crassostrea gigas, C-angulata and their hybrids
Provedor de dados: 5
Autores: Soletchnik, Patrick; Huvet, Arnaud; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Faury, Nicole; Goulletquer, Philippe; Boudry, Pierre.
This paper reports a comparative study of two cupped oysters, Crassostrea gigas and C angulata, and their hybrids reared in the Marennes-Oleron Sound (France) under usual farming conditions. The relative proficiency of these species was characterized in terms of growth, survival and reproduction, three major concerns for oyster farming. Four populations of each taxon were sampled (C gigas: Japan and France; C angulata: Taiwan and Spain) and used as progenitors to produce six different progenies under common hatchery and nursery conditions (transfer of 5 to 6 g of seed oysters at sea and rearing in bags for 2 years). Mortality profiles differed greatly among the progenies. The C angulata progeny of Taiwanese origin gave the poorest results, with 100%...
Tipo: Text
Palavras-chave: Atlantic coast; Crassostrea gigas; Phenotypes; Hybrids; Oyster fanning.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-560.pdf

Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae
Provedor de dados: 5
Autores: Foulon, Valentin; Artigaud, Sebastien; Buscaglia, Manon; Bernay, Benoit; Fabioux, Caroline; Petton, Bruno; Elies, Philippe; Boukerma, Kada; Hellio, Claire; Guerard, Fabienne; Boudry, Pierre.
Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00462/57379/59452.pdf

Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality
Provedor de dados: 5
Autores: David, Elise; Boudry, Pierre; Degremont, Lionel; Tanguy, Amelie; Quere, N; Samain, Jean-francois; Moraga, D.
Large-scale mortality events have been observed in Pacific oyster Crassostrea gigas on the west coast of France since the early 1980s, particularly during summer. In order to understand the causes of this mortality, two generations of oysters from single-pair matings were studied in three sites on the French Atlantic coast (Baie-des-Veys, Auray and Ronce-les-Bains). The present paper examines the role of two candidate genes in the susceptibility of oysters to summer mortality, and the selective pressure exerted by such mortality on their polymorphism. Glutamine synthetase (amino-acid metabolism) and delta-9 desaturase (lipid metabolism) genes were studied in the successive generations, using polymerase chain reaction single-strand conformation polymorphism...
Tipo: Text
Palavras-chave: SSCP; Mortality; Glutamine synthetase; Delta 9 desaturase; Crassostrea gigas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3040.pdf

Sustainable large‐scale production of European flat oyster (     Ostrea edulis     ) seed for ecological restoration and aquaculture: a review
Provedor de dados: 5
Autores: Colsoul, Bérenger; Boudry, Pierre; Pérez‐parallé, María Luz; Bratoš Cetinić, Ana; Hugh‐jones, Tristan; Arzul, Isabelle; Mérou, Nicolas; Wegner, Karl Mathias; Peter, Corina; Merk, Verena; Pogoda, Bernadette.
The conservation and active restoration of European flat oyster (Ostrea edulis) populations are a major focus of ecological restoration efforts to take advantage of the wide‐ranging ecosystem functions and services this species provides. Accordingly, additional and new demands for seed oysters have arisen. In commercial aquaculture (mariculture), the production of O. edulis is still largely based on natural seed collection. Considering the specific requirements, related to ecological restoration, such as the absence of pathogens and the preservation of high genetic diversity, the current supply is insufficient. Despite the development of breeding and controlled reproduction techniques for this species since the late 1930s, seed production today is mainly...
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00678/79001/81394.pdf

Impacts of ocean acidification and warming stress on juvenile growth and metabolism in two populations of Pecten maximus
Provedor de dados: 5
Autores: Harney, Ewan; Rastrick, Samuel; Artigaud, Sébastien; Pisapia, Julia; Miner, Philippe; Pichereau, Vianney; Strand, Oivind; Boudry, Pierre; Charrier, Gregory.
Elevated atmospheric CO2 is a major driver of global change in the ocean, causing sea surface temperatures to rise and acidification of marine environments. Together these stressors may have additive, synergistic or unforeseen consequences for many marine organisms, particularly during early life stages. The economically important King Scallop (Pecten maximus) is found along a large latitudinal gradient in the Eastern Atlantic, but it is unclear how this species will respond to changing environmental conditions. Studies of genetic structure suggest that Atlantic populations (from Spain to the UK) are genetically distinct from Norwegian populations, a result that is borne out by phenotypic differences. Yet whether genetic differences arise as a result of...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00404/51520/52114.pdf

Past and present geographical distribution of populations of Portuguese (Crassostrea angulata) and Pacific (C. gigas) oysters along the European and north African Atlantic coats
Provedor de dados: 5
Autores: Fabioux, Caroline; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre.
Today, it is commonly accepted that C. angulata and C. gigas are a single species according to morphological, genetic and experimental hybridization data. Following the viral disease that affected C. angulata and the subsequent expansion of C. gigas in Europe, it was of interest to examine the geographical distribution of both taxa in Europe. We studied the genetic composition of seventeen populations of cupped oysters, sampled in France, Spain, Portugal, Morocco and Italy, using a diagnostic marker, the mitochondrial Cytochrome Oxydase I gene. Results showed two distinct geographical zones. All the populations located in the northern part of the European sampled zone were mainly composed of C. gigas haplotypes whereas populations from southern Europe and...
Tipo: Text
Palavras-chave: Geographical distribution; Crassostrea angulata; Crassostrea gigas; Distribution géographique; Crassostrea angulata; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2785.pdf

"Bivalvomix" : génomique évolutive des bivalves marins
Provedor de dados: 5
Autores: Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice.
For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang &amp; Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf

Différenciation génétique des populations d'huître plate européenne, Ostrea edulis, à l'aide de marqueurs des génomes mitochondrial et nucléaire
Provedor de dados: 5
Autores: Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois.
Two oyster species are cultivated on the French coasts: the Pacific cupped oyster (Crassostrea gigas), which has been introduced from Japan in the 1970's after the disappearance of the Portuguese oyster Crassostrea angulala, and the European flat oyster (Ostrea edulis). During the second half of the 1960's, the latter suffered successively from two diseases caused by protozoan parasites: marteiliosis (also called "maladie des Abers") and bonamiosis. In France, its production dropped from 20,000 tian in the 1960's to 2,000 today. The impact of these two pathogens as well as the exploitation of the remaining stocks show the importance of the conservation of this species, the only natural European species, whose population is more and more fragmented.
Tipo: Text
Palavras-chave: Marqueurs; Génétique population; Génétique; Ostrea edulis; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3273.pdf

Experimental evidence for a genetic basis to differences in aneuploidy in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Leitao, Alexandra; Boudry, Pierre; Mccombie, Helen; Gerard, Andre; Thiriot-quievreux, Catherine.
Aneuploidy has been previously reported in wild and cultivated Pacific oysters, Crassostrea gigas, and has been shown to be negatively correlated with growth. This is especially important since high variability of growth rate is one of the major problems in the aquacultural production of this species. The existence of a genetic basis for the observed differences in aneuploidy was first investigated through the comparative study of six full-sib families, with mean individual weights ranging from 0.59 to 1.49 g. The slowest growing family was found to have the highest level of aneuploidy. Significant differences in aneuploidy were also found among families when individuals with the same growth rate were sampled. This supports the hypothesis of the existence...
Tipo: Text
Palavras-chave: Oyster; Growth; Genetics; Full sib families; Crassostrea gigas; Aneuploidy; Huître; Croissance; Génétique; Famille de plein frères; Crassostrea gigas; Aneuploïdie.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/2001/publication-2700.pdf

Variance in the reproductive success of flat oyster Ostrea edulis L. assessed by parentage analyses in natural and experimental conditions
Provedor de dados: 5
Autores: Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie.
In order to document further the phenomena of variance in reproductive success in natural populations of the European flat oyster Ostrea Midis, two complementary studies based on natural and experimental populations were conducted. The first part of this work was focused on paternity analyses using a set of four microsatellite markers for larvae collected from 13 brooding females sampled in Quiberon Bay (Brittany, France). The number of individuals contributing as the male parent to each progeny assay was highly variable, ranging from 2 to more than 40. Moreover, paternal contributions showed a much skewed distribution, with some males contributing to 50-100% of the progeny assay. The second part of this work consisted of the analysis of six successive...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00012/12329/9222.pdf

De novo assembly and annotation of the European abalone Haliotis tuberculata transcriptome
Provedor de dados: 5
Autores: Harney, Ewan; Dubief, Bruno; Boudry, Pierre; Basuyaux, Olivier; Schilhabel, Markus B.; Huchette, Sylvain; Paillard, Christine; Nunes, Flavia.
The European abalone Haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. Aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic Vibrio infection. Transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. To generate an extensive transcriptome, we performed next-generation sequencing of RNA on larvae exposed to temperature and pH variation and on haemolymph of adults from two wild populations after experimental infection with Vibrio harveyi. We obtained more than 1.5...
Tipo: Text
Palavras-chave: RNA-Seq; Mollusca; Gastropoda; Development; Vibrio harveyi; Climate change.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00318/42940/45103.pdf

The use of SNPs in characterizing oysters genomes and their resistance to pathogens
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Harrang, Estelle; Morga, Benjamin; Flahauw, Emilie; Sauvage, Christopher; Boudry, Pierre.
During the last five years, a great effort has been made to develop genomic tools in oyster species all over the world. In Europe, the Pacific cupped oyster, Crassostrea gigas, and the European flat oyster, Ostrea edulis received a special attention as they are the key species for local aquaculture. In particular, the Pacific oyster is a marine bivalve of major economical and ecological importance worldwide. Better knowledge of its physiology and its genetics contributes to optimize its aquaculture production using selective breeding or to understand its invasive behavior in some coastal areas. Current international developments of genomic resources include EST databases, BAC libraries, genome mapping and sequencing. The Genoscope (Evry, France) and the...
Tipo: Text
Palavras-chave: Oysters; Crassostrea gigas; Ostrea edulis; Resistance pathogens; QTLs; Single Nucleotide Polymorphism.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12584/9454.pdf

Phylogeny and phylogeography of Atlantic oyster species: evolutionary history, limited genetic connectivity and isolation by distance
Provedor de dados: 5
Autores: Lazoski, C.; Gusmao, J.; Boudry, Pierre; Sole-cava, A. M.
The phylogenetic relationships between naturally occurring Atlantic Crassostrea oyster species were inferred through analyses of mitochondrial (cytochrome oxidase subunit I and 16S) and nuclear (second internal transcribed spacer) sequences. We also scored 15 allozyme loci on 422 oysters to study population structuring of C. rhizophorae and C. brasiliana along 9000 km of the Western Atlantic coastline. Despite morphological similarities, C. virginica was genetically more closely related to C. rhizophorae than to C. brasiliana. In contrast, C. paraibanensis was genetically indistinguishable from C. brasiliana, which is probably a junior synonym of the African C. gasar. Significant genetic differentiation between populations of C. rhizophorae and C. gasar...
Tipo: Text
Palavras-chave: Population genetics; Biogeography; Allozymes; Cytochrome c oxidase subunit I; COI; 16S; Internal transcribed spacer 2; ITS-2.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14464/11765.pdf

Bases génétiques et variabilité des caractères physiologiques impliqués dans la croissance de Crassostrea gigas
Provedor de dados: 5
Autores: Baud, Jean-pierre; Palvadeau, Hubert; Nourry, Max; Haure, Joel; Penisson, Christian; Boudry, Pierre.
La production française d' huître creuse Crassostrea gigas représente, avec 144000 tonnes de production, la 4ème' place au ni veau mondial après la Chine (2 285 000 t), le Japon (225 000t.) et la Corée (1 85 000t.). Ce bivalve filtreur possède un potentiel d'adaptation élevé puisqu'on le trouve sous des latitudes très diverses. Malgré son importance économique mondiale (2 900 000 tonnes de production en 1996), à ce jour, très peu de programmes de sélection ont été mis en oeuvre sur cette espèce, qui n'est pas considérée comme domestiquées au sens génétique du terme. Toutefois, avant de lancer des programmes - nécessairement de grande ampleur - pour la sélection de souches d' huîtres creuses Crassostrea gigas, il est utile de répondre au préalable à...
Tipo: Text
Palavras-chave: Génétique; Variabilité génétique; Croissance; Huîtres creuses; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00048/15881/13303.pdf

Rapport d’activité 2018-2019 UNITE RBE/PFOM. Physiologie Fonctionnelle des Organismes Marins
Provedor de dados: 5
Autores: Boudry, Pierre; Fleury, Elodie; Le Roux, Frederique; Zambonino Infante, Jose-luis.
Activités scientifiques 2018-2019
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00689/80155/83222.pdf

Nutrition and reproduction are key parameters in the interaction process leading to Crassostrea gigas oyster summer mortality in France
Provedor de dados: 5
Autores: Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre.
Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence...
Tipo: Text
Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy.
Ano: 2006
URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf

Expression of candidate genes related to metabolism, immunity and cellular stress during massive mortality in the American oyster Crassostrea virginica larvae in relation to biochemical and physiological parameters
Provedor de dados: 5
Autores: Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean.
Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested...
Tipo: Text
Palavras-chave: Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00075/18585/16138.pdf

Mitochondrial and microsatellite genetic differentiation of the flat oyster Ostrea edulis along the european coasts, and comparison with allozyme data
Provedor de dados: 5
Autores: Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois.
Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in  the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes.
Tipo: Text
Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf

Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Tanguy, Arnaud; Boutet, Isabelle; Boudry, Pierre; Degremont, Lionel; Laroche, Jean; Moraga, Dario.
Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA...
Tipo: Text
Palavras-chave: Gene expression; Xenobiotics; Mollusc; Phosphoglucomutase.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2220.pdf

Phylogéographie des huîtres de mangrove de l'Océan Atlantique Sud : C. gasar et C. rhisophorae
Provedor de dados: 5
Autores: Heurtebise, Serge; Boutet, Isabelle; Verden, B.; Lapegue, Sylvie; Leitao, Alexandra; Thiriot-quievreux, Catherine; Garica, P.; Boudry, Pierre.
Mangrove oysters include various species that are also present on different coasts. In the southern part of the Atlantic Ocean, Crassostrea rhizophorae has been observed along the southern coast of America and Crassostrea gasar along the western coast of Africa. Fifteen populations of these oysters were sampled from these two geographic areas. Their polymorphism was studied on the 16S mitochondrial fragment both by par sequencing and RFLP analysis. Some samples' caryotype were also analysed. Two haplotypes were identified: C. gasar was found in Africa, but also for the first time in South America, whereas C. rhizophorae was only found on the coasts of South America.
Tipo: Text
Palavras-chave: Crassostrea rhizophorae; Crassostrea gasar; Huîtres; Génétique; Phylogéographie.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3278.pdf

A preliminary genetic linkage map for the European flat oyster Ostrea eduli
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Halley, Chris; Boudry, Pierre; Beaumont, Andy.
The European flat oyster, Ostrea edulis, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and throughout the Mediterranean. The oyster has been a harvested species for at least 6000 years. However, it successively suffered from over harvesting and then from two protozoan diseases during the 1960s and its production has drastically decreased in Europe from that time. Following two generations of selection for tolerance to one of these diseases (bonamiosis), our aim is to optimize this selection with the assistance of molecular markers, that is to say to implement marker-assisted selection. This will enhance the efficiency of selection by reducing the generation time, as the selection on sites cannot be made before the oysters are...
Tipo: Text
Palavras-chave: QTLs; Ostrea edulis; Genetic linkage map; Genetic; European flat oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3473.pdf

Utilisation des marqueurs microsatellites pour l'étude des contributions parentales chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Kotoulas, G.; Hervouet, Veronique; Cornette, Florence; Bonhomme, Francois; Gerard, Andre.
Numerous aquacultural species show very high fertilities. As a consequence, it is possible to produce, in hatcheries, a large amount of sibs from a small amount of parents. The sibs' genetic variability is, of course, directly linked to the number of parents involved, but it may be largely lowered by imbalanced parental contributions. Polymorphic genetic markers are very handy tools in the study of these phenomena in cultivated populations (Harris et al. 1991; Herbinger et al., 1996a; Garcia de Leon et al. 1998) or in natural populations (Ferguson et al, 1995; Kellog et al., 1995; Colbourne et al., 1996; Herbinger et al., 1996b). This document is a presentation of the results of a number of experiments, realised in the scope of the European program...
Tipo: Text
Palavras-chave: Microsatellites; Contributions parentales; Crassostrea gigas; Huîtres.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3452.pdf

Evidence for the presence of the Portuguese oyster, Crassostrea angulata, in northern China
Provedor de dados: 5
Autores: Lapegue, Sylvie; Batista, Frederico; Heurtebise, Serge; Yu, Ziniu; Boudry, Pierre.
The Pacific oyster, Crassostrea gigas (Thunberg), and the Portuguese oyster, C. angulata (Lamarck), are two closely related taxa. Although these two taxa were both introduced from Asia into Europe. one (C. gigas) was voluntarily introduced in the early 1970s, whereas the other (C. angulata) was presumed to be present in Europe for at least four centuries, but nearly disappeared because of disease. Few C. angulata populations remained in southern Portugal, Spain and Morocco and their putative origin was traced in Taiwan. The present paper reports evidence for its presence in Northern China. We reanalyzed recently published mitochondrial cytochrome oxidase C subunit I (COI) sequence data from presumed Dalianwan oysters (C. talienwhanensis) and compared them...
Tipo: Text
Palavras-chave: Phylogeography; Cytochrome oxidase C subunit 1 gene; Crassostrea talienwhanensis; Gigas; Crosstrea; Crassostrea angulata; Cupped oysters.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-3173.pdf

An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas
Provedor de dados: 5
Autores: Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud.
This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,...
Tipo: Text
Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf

Ressources génétiques chez l'huître creuse Crassostrea gigas : variabilité, différenciation et adaptation aux conditions de production en Charente Maritime
Provedor de dados: 5
Autores: Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie.
es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)...
Tipo: Text
Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf

Physiological and molecular basis of gametogenesis in triploid Pacific oysters, Crassostrea gigas
Provedor de dados: 5
Autores: Normand, Julien; Huvet, Arnaud; Fabioux, Caroline; Raguenes, Morgane; Boudry, Pierre.
Genetic improvement of the Pacific oyster, Crassostrea gigas, can be achieved through triploidisation, which leads to partially sterility, allowing higher growth and survival together with improved meat quality during the reproductive period. Most triploids are not fully sterile and the genetic and environmental factors involved in their partial fertility remained to be investigated.  We examined gametogenesis by image analysis of histological cross sections in diploid (2n) and triploid oysters obtained either by crossing tetraploids and diploid broodstock (« natural » triploids: 3nn) or from second polar body retention (« chemical » triploids: 3nc).  5-month old triploid spat reared in a standard intensive nursery system only showed a reduction of 50% of...
Tipo: Text
Palavras-chave: Gametogenesis; Triploid; Crassostrea gigas; Genetic; Oysters.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3327.pdf

Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas)
Provedor de dados: 5
Autores: Sauvage, Christopher; Lapegue, Sylvie; Bierne, Nicolas; Boudry, Pierre.
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). The identification of markers associated to the resistance would allow to better understand this complex phenomenon and, eventually to establish markers assisted selection. Recently, two moderately dense genetic linkage maps have been established in the Pacific oyster based on microsatellites and AFLP markers (Hubert and Hedgecock, 2004; Li and Guo, 2004). In this context, the development and mapping of SNPs will increase the density of the linkage map and consequently the...
Tipo: Text
Palavras-chave: QTLs analyses; Summer mortaliy; Genetic; SNPs; Crassostrea gigas; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3351.pdf

Overwiew on molecular tools in bivalve shellfish aquaculture
Provedor de dados: 5
Autores: Boudry, Pierre; Saavedra, Carlos.
Dr. Boudry presented an overview of the current development of molecular tools in shellfish bivalves, focussing on the recent development in oysters, species to which the most efforts are dedicated. This review was prepared together with Dr. Saavedra (Mollusc Research Group, Torre de la Sal, Spain) within the Fish and Shellfish group of the EU Network of Excellence "Marine Genomics Europe". Searches in DNA sequence databank ("GenBank") show that 4% are microsatellite sequences, 23% are mitochondrial sequences and the remaining 73 % other nuclear sequences. Among these, 75% concern oyster (Crassostrea) species, 15% mussel (Mytilus) species and 10% scallop (Argopecten) species. Some of these sequences were targeted as candidate genes related to known...
Tipo: Text
Palavras-chave: QTLs; Mitochondrial geneome; Crassostrea virginica; Crassostrea gigas; Crassostrea; Shellfish bivalves; Molecular tools; Genetic.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3480.pdf

Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane.
To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to...
Tipo: Text
Palavras-chave: Ingestion; Gene expression; Bivalve; Alpha amylase.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-5165.pdf

Effects of genetic drift and selection at larval stage resulting from hatchery practices in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Batista, Frederico; Boudry, Pierre.
Hatchery techniques are now well handled in an increasing number of aquaculture species. However, unintentional effects of domestication often remain poorly documented. In this context, we studied how current hatchery practices may genetically influence larval traits for which significant genetic variation had previously been reported in the Pacific oyster. Two main factors were studied: culling (i.e. discarding the smallest larvae) and rearing temperature (26°C versus 20°C). In parallel, we estimated genetic diversity of broodstock populations sampled in French hatcheries. Finally, we compared larval development and settlement success in the progeny of one of these hatchery broodstock, which had been selected for seven generations, with those of wild...
Tipo: Text
Palavras-chave: Genetic drift; Selection; Genetic; Crassostrea gigas; Oysters; Hatchery techniques.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3362.pdf

Reproductive effort and growth in Crassostrea gigas: comparison of young diploid and triploid oysters issued from natural crosses or chemical induction
Provedor de dados: 5
Autores: Normand, Julien; Ernande, Bruno; Haure, Joel; Mccombie, Helen; Boudry, Pierre.
Early reproductive effort and growth were measured in 3 groups of the Pacific oyster Crassostrea gigas: 1 diploid group and 2 triploid groups resulting from either chemical induction (3nCB) or crosses between tetraploid and diploid parents (3nDT). Oysters were reared under intensive nursery conditions and sampled when 5 mo old. Reproductive effort was estimated by cross-sectional area measurements of the visceral mass (i.e. gonadic occupation) and maturation stage was assessed by qualitative histology. As expected, comparison of the reproductive patterns of these 3 groups revealed a lower reproductive effort in triploid individuals relative to diploid. However, gonadic occupation in triploid oysters was higher than expected in both 3nCB and 3nDT groups, as...
Tipo: Text
Palavras-chave: Triploidy; Oyster; Gametogenesis; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6978.pdf

Mitochondrial and nuclear DNA phylogeography of  two cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud.
The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data...
Tipo: Text
Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf

Genetic improvement and selection in shellfish : a review based on oyster research and production
Provedor de dados: 5
Autores: Boudry, Pierre; Barre, Marc; Gerard, Andre.
Despite the economic importance of shellfish, genetic improvement has not yet had a great impact on these species. Shellfish farming is traditionally based on wild species whose natural populations are often overexploited and do not fulfill market demand. Up to now, the most effective answer to disease problems or for the improvement of productivity has been the introduction of new species. However, the introduction of new species is constrained by their ecological impact and is also limited by the availability of suitable species. Genetic improvement of local species should therefore be of great importance as a viable alternative for the long term sustainability of the shellfish industry. The different approaches to genetic improvement include...
Tipo: Text
Palavras-chave: Selective breeding; Hybridization; Polyplidy; Introduction; Oysters; Selection; Hybridation; Polyploidie; Introduction; Huitres.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3499.pdf

Cytogenetic study of Ostrea conchaphila (Mollusca : Bivalvia) and comparative karyological analysis within Ostreinae
Provedor de dados: 5
Autores: Leitao, Alexandra; Chaves, Raquel; Santos, Sara; Boudry, Pierre; Guedes Pinto, Henrique; Thiriot Quievreux, Catherine.
Chromosome preparations of the Olympia oyster Ostrea conchaphila Carpenter were studied using conventional Giemsa, silver staining, and C-banding techniques. The karyotype consists of six metacentric (1, 2, 4, 6, 8, and 10) and four submetacentric (3, 5, 7, 9) chromosome pairs. The silver-stained nucleolus organizer regions (Ag-NORs) were terminally located on the short arms of the submetacentric pair 5 (56% of cases) and on the long arms of submetacentric pair 7 (6% of cases). Constitutive heterochromatin was observed as telomeric C-bands on the short arm of the NOR-bearing chromosome pair 5 and as centromeric blocks of several chromosome pairs. Comparative analysis of patterns of karyotype, Ag-NORs, and C-bands of this species and of five other flat...
Tipo: Text
Palavras-chave: Ostreinae; Cytotaxonomy; Cytogenetics; Ostrea conchaphila.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/2002/publication-2792.pdf

Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of  Crassostrea gigas,  the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text
Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf

Pacific cupped oyster - Crassostrea gigas
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boudry, Pierre; Goulletquer, Philippe.
Originating from the north eastern Asia, Crassostrea gigas is endemic to Japan, but has been introduced and translocated, mainly for aquaculture purpose, into several countries, almost worldwide (1). In North America, the species can be found from Southeast Alaska to Baja California, while in European waters the species is cultured from Norway to Portugal as well as in Mediterranean Sea (Fig.1) (2). Biological characteristics make it suitable for a wide range of environmental conditions, although it is usually found in coastal and estuarine areas within its natural range. Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (3).
Tipo: Text
Palavras-chave: Genetics; Ecology; Crassostrea gigas; Biology; Oysters.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3322.pdf

Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (slides)
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Oysters, like many marine bivalves, are characterized by very high fecundity and low survival rate at early stages of life. Rearing conditions in hatcheries (i.e. density, feeding, temperature...) are different from those observed in the wild. Additionally, slow growing larvae are culled by sieving. As previous studies have shown that genetic variability exists for several early development traits, we aimed to assess if common hatchery rearing practices exert specific selective processes at the larval stage. In order to avoid possible bias and to increase the number of families studied, we used a mixed-family approach with subsequent parentage analysis using a recently developed set of three multiplexed microsatellite markers. We studied the variance in...
Tipo: Text
Palavras-chave: Hatcheries; Selection; Genetic drift; Genetic; Crassotrea gigas; Pacific oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3483.pdf

Genetic improvement and selection in shellfish : a review based on oyster research and production
Provedor de dados: 5
Autores: Boudry, Pierre; Barre, Marc; Gerard, Andre.
Despite the economic importance of shellfish, genetic improvement has not yet had a great impact on these species. Shellfish farming is traditionally based on wild species whose natural populations are often overexploited and do not fulfil market demand. Up to now, the most effective answer 10 disease problems or for the improvement of productivity has been the introduction of new species. However, the introduction of new species is constrained by their ecological impact and is also limited by the availability 01 suitable species. Genetic improvement of local species should therefore be of great importance as a viable alternative for the long term sustainability 01 the shellfish Industry. The different approaches 10 genetic improvement include...
Tipo: Text
Palavras-chave: Selective breeding; Hybridization; Polyploidy; Introduction; Oyter; Sélection; Hybridation; Polyploïdie; Introduction; Huîtres.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-2740.pdf

Genetic effect of domestication selective pressures on Pacific oyster at larval stage
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Among bivalve species, the Pacific oyster, Crassostrea gigas, is the most economically important bivalve production over the world. Today, C. gigas is subject to an important production effort that leads to an intensive artificial selection. Larval stage is relatively unknown, specifically in a domestication context. Genetic consequence of artificial selection is still at a preliminary study. We aimed to tackle the consequence of inconscient domestication on the variance reproductive success focusing on larval stage, keystone of the life cycle. We studied two kinds of specific selective processes that common hatchery rearing practices exert : the effect of discarding the smallest larvae on genetic diversity and the artificial environment rearing effect via...
Tipo: Text
Palavras-chave: Selection; Genetic correlations; Genetic variability; Genetic; Domestication selective; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3441.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridations et conservatoire de souches. Contrat de Plan Etat Région Poitou- Charentes 1994-1998 - Convention 95/RPC-R-57 "Génétique" - Rapport année 1995
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Heurtebise, Serge; Delsert, Claude; Ledu, Christophe; Phelipot, Pascal; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous les membres du Réseau Génétique Mollusques (REGEMO), a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou-Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses.
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea virgnica.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00044/15515/12902.pdf

European flat oyster - Ostrea edulis
Provedor de dados: 5
Autores: Lapegue, Sylvie; Beaumont, A.; Boudry, Pierre; Goulletquer, Philippe.
The European flat oyster, Ostrea edulis, a native of Europe, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and in the whole Mediterranean Basin; It has been a harvested species for at least 6000 years. Natural populations are also observed in eastern North America, from Maine to Rhode Island, following intentional introductions in the 1940s and 1950s. The species was also introduced in Canada for aquaculture purpose 30 years ago and some populations naturalised in Nova Scotia, New Brunswick and British Columbia. These stocks were imported from naturalised populations in Maine whose ancestors originated in the Netherlands.
Tipo: Text
Palavras-chave: Genetics; Ecology; Biology; Ostrea edulis; Oysters.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3321.pdf

Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). I. Estimation of genetic parameters for survival and growth
Provedor de dados: 5
Autores: Degremont, Lionel; Ernande, Bruno; Bedier, Edouard; Boudry, Pierre.
The multidisciplinary project "MOREST" aims to improve our understanding of causes of summer mortality in Crassostrea gigas juveniles in France and to reduce its impact on oyster production. As pail of the MOREST project, 43 full-sib families nested within 17 half-sib families were produced, planted out and tested in 3 sites during summer 2001 to assess to what extent genetic variability exists for this trait. A strong genetic basis was found for survival as narrow-sense heritability estimates ranged from 0.47 to 1.08, with higher values in sites where summer mortality was higher. Genetic correlations across sites were positive and very high for survival, indicating no genotype by environment interaction. In contrast, lower genetic variation was observed...
Tipo: Text
Palavras-chave: Growth; Survival; Juveniles; Heritability; Crassostrea gigas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2457.pdf

Research and development for the genetic improvement of the Pacific oyster in France : present and prospects
Provedor de dados: 5
Autores: Boudry, Pierre; Haffray, Pierrick.
The most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) in France to date has been through the breeding of triploids, especially since the development of tetraploids. Triploid oysters commonly resist summer mortality better than diploids. This is most probably because reproductive allocation is so important in this phenomenon. Quantitative genetics studies strongly suggest that a significant gain in resistance to summer mortality, or other traits of interest, could be obtained by selective breeding. Practical difficulties however, and the high cost of breeding large numbers of families under common conditions over many generations remain major constraints to family-based selective breeding in oysters. In the last...
Tipo: Text
Palavras-chave: Polyploidization; Selective breeding; Genetic; Crassostrea gigas; Pacific oysters.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3438.pdf

Genetic parameters of resistance to Vibrio aestuarianus, and OsHV-1 infections in the Pacific oyster, Crassostrea gigas, at three different life stages
Provedor de dados: 5
Autores: Azema, Patrick; Lamy, Jean-baptiste; Boudry, Pierre; Renault, Tristan; Travers, Marie-agnes; Degremont, Lionel.
Background  In France, two main diseases threaten Pacific oyster production. Since 2008, Crassostrea gigas spat have suffered massive losses due to the ostreid herpesvirus OsHV-1, and since 2012, significant mortalities in commercial-size adults have been related to infection by the bacterium Vibrio aestuarianus. The genetic basis for resistance to V. aestuarianus and OsHV-1 and the nature of the genetic correlation between these two traits were investigated by using 20 half-sib sire families, each containing two full-sib families. For each disease, controlled infectious challenges were conducted using naïve oysters that were 3 to 26 months old. In addition, siblings were tested under field, pond and raceway conditions to determine whether laboratory...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00372/48350/48518.pdf

Review and practical use of genome mapping in aquacultured organisms. Position paper by the Working Group on the Application of Genetics in Fisheries and Mariculture (WGAGFM)
Provedor de dados: 5
Autores: Boudry, Pierre; Lapegue, Sylvie; Guyomard, R.
The Working Group on the Application of Genetics in Fisheries and Mariculture (WGAGFM) met in La Rochelle, France from 10-12 March, 2003. Seven persons representing six countries were present. Three terms of reference (ToR) were addressed, while one was deferred until the appropriate information was available for review. WGAGFM first considered ToR a) Review and report on the practical use of genome mapping in aquacultured organisms. Genome maps are used extensively in the production of selected strains of agriculture plants and livestock. Studies on the genome of aquatic animais have been summarised in the WG report. Genome mapping projects in aquaculture exist for most major species and linkage maps are now becoming available. WGAGFM produced four...
Tipo: Text
Palavras-chave: Conservation of genetic diversity; Triploids; Recommandation; Aquaculture; Fish; Genome mapping; Genetic.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3301.pdf

Scanning the genome of the Pacific cupped oyster, Crassostrea gigas, for adaptative differentiation during geographic range expansion
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf

Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
Provedor de dados: 5
Autores: Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf

Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes)
Provedor de dados: 5
Autores: Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,...
Tipo: Text
Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf

Detailed insights into pan-European population structure and inbreeding in wild and hatchery Pacific oysters (Crassostrea gigas) revealed by genome-wide SNP data
Provedor de dados: 5
Autores: Vendrami, David L. J.; Houston, Ross D.; Gharbi, Karim; Telesca, Luca; Gutierrez, Alejandro P.; Gurney-smith, Helen; Hasegawa, Natsuki; Boudry, Pierre; Hoffman, Joseph I.
Cultivated bivalves are important not only because of their economic value, but also due to their impacts on natural ecosystems. The Pacific oyster (Crassostrea gigas) is the world's most heavily cultivated shellfish species and has been introduced to all continents except Antarctica for aquaculture. We therefore used a medium-density single nucleotide polymorphism (SNP) array to investigate the genetic structure of this species in Europe, where it was introduced during the 1960s and has since become a prolific invader of coastal ecosystems across the continent. We analyzed 21,499 polymorphic SNPs in 232 individuals from 23 localities spanning a latitudinal cline from Portugal to Norway and including the source populations of Japan and Canada. We confirmed...
Tipo: Text
Palavras-chave: Aquaculture; Crassostrea gigas; Genetic structure; High-density genotyping array; Inbreeding; Pacific oyster; Restriction site-associated DNA (RAD) sequencing; Single nucleotide polymorphism (SNP).
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00484/59561/62557.pdf

NORA moving forward: Developing an oyster restoration network in Europe to support the Berlin Oyster Recommendation
Provedor de dados: 5
Autores: Pogoda, Bernadette; Boudry, Pierre; Bromley, Cass; Cameron, Tom C.; Colsoul, Bérenger; Donnan, David; Hancock, Boze; Hugh‐jones, Tristan; Preston, Joanne; Sanderson, William G.; Sas, Hein; Brown, Janet; Bonacic, Kruno; Nordheim, Henning; Zu Ermgassen, Philine S.e.
The Native Oyster Restoration Alliance (NORA) supports the protection and ecological restoration of the native European oyster, Ostrea edulis, and its habitat across its current and historical biogeographical range. NORA works to overcome barriers to the conservation, restoration, and recovery of the European oyster by providing a platform for the NORA community to collaborate and participate in knowledge exchange. NORA seeks to support responsible restoration practice, in compliance with biosecurity and sustainability. Against this background, the NORA community formulated a series of specific recommendations, the Berlin Oyster Recommendation, to support native oyster restoration by developing and applying best practice with the aim to recover healthy and...
Tipo: Text
Palavras-chave: Ecological restoration; Native Oyster Restoration Alliance; Ostrea edulis.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00659/77135/78451.pdf

Etude du niveau d'aneuploïdie dans les populations des zones de captage du bassin de Marennes-Oléron - Contrat Plan Etat Région Poitou-Charentes 1999 - Rapport 1999
Provedor de dados: 5
Autores: Lapegue, Sylvie; Thiriot Quievreux, Catherine; Mccombie, Helen; Heurtebise, Serge; Boudry, Pierre; Robert, Stephane; Soletchnik, Patrick; Goulletquer, Philippe; Gerard, Andre.
Divers travaux, menés depuis 1984, montrent qu'il existe, chez l'huître creuse Crassotrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Le but de l'expérimentation menée en 1999 est de faire un...
Tipo: Text
Palavras-chave: Génétique; Aneuploïdie; Génétique des populations; Biochimie; Marennes-Oléron; Huître creuse; Crassostrea gigas.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00044/15522/12909.pdf

Chromosome loss in tetraploid families of the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre.
Alterations of  chromosome number have been observed in the somatic tissue of  Crassostrea gigas diploids and artificial polyploids bred in this species.   Tetraploid animals with lower chromosome counts in some or all of their somatic tissue are considered undesirable as parents either for commercial triploids (produced via a  cross with diploids) or for tetraploid breeding because (1) they may confer the tendency to lose chromosomes and revert to lower ploidy levels to their offspring or (2) their offspring could have a lower ploidy directly because of potential links between somatic and gametic chromosome loss.  The present study evaluated the phenomenon in bi-parental tetraploid families bred from parents of differing ploidy quality, including a...
Tipo: Text
Palavras-chave: Tetraploid; Chromosome; Genetic; Crassostrea gigas; Oyster.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3435.pdf

Physiological and biochemical changes associated with massive mortality events occurring in larvae of American oyster (Crassostrea virginica)
Provedor de dados: 5
Autores: Genard, Bertrand; Pernet, Fabrice; Lemarchand, Karine; Boudry, Pierre; Moraga, Dario; Tremblay, Rejean.
In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of...
Tipo: Text
Palavras-chave: Massive mortality; Larvae; Microbial environment; Lipid; Fatty acids; Energy metabolism; Oxidative stress; Antioxidant enzymes; Crassostrea virginica.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00051/16231/13753.pdf

Ressources génétiques chez les huîtres creuses : conservatoire de souche et marqueurs moléculaires
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Collet, Bertrand; Cornette, Florence; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
The cupped oyster strain bank was created at Ifremer at La Tremblade in 1992. It was funded by the Poitou-Charentes regional council. Its goals were: 1. to study the intra- and interspecific genetic differentiation within the genus Crassostrea. 2. to study acclimatization potential of foreign oysters in French waters in order to identify species that could replace Crassostrea gigas in case of a major disease outbreak in C. gigas or in case of a need or desire to diversify shellfish production. 3. to study the possibility of interspecific hybridisation in the genus Crassostrea and hybrid performance.
Tipo: Text
Palavras-chave: Saccostrea commercialis; Crassostrea rhisophorae; Crassostrea gasar; Crassostrea sikamea; Crassostrea virginica; Crassostrea rivularis; Crassotea angulata; Crassostrea gigas; Génétique.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3242.pdf

Contrôle de la gamétogénèse des huîtres creuses et plates. Relations "reproduction" et "génétique"
Provedor de dados: 5
Autores: Gerard, Andre; Naciri-graven, Yamama; Boudry, Pierre; Launey, Sophie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal.
At the end of the 1980s, after two decades of aquaculture research in molluscs, Ifremer initiated its first genetic studies. These new studies quickly revealed the limits of knowledge in aquaculture, particularly in terms of controlling oyster reproduction. This presentation attempts to summarize the available information on genetics in aquaculture. In particular, breeding practices in the 1980s involved populations of broodstock, while genetics requires modern techniques where individuals, not just populations, can be identified. Controlling reproduction at the individual level in oysters raises new questions that existing protocols cannot address, especially in terms of controlling sex determination, individual maturity, spawning synchrony, chemical...
Tipo: Text
Palavras-chave: Gamètes; Fécondation; Induction ponte; Reproducteur; Conditionnement; Gamétogénèse; Génétique; Reproduction; Crassostrea gigas; Huîtres.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-3210.pdf

Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text
Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6421.pdf

Domestication modifies behaviour of first generation of domesticated abalone
Provedor de dados: 5
Autores: Roussel, Sabine; Bish, Thomas; Day, Rob; Boudry, Pierre; Huchette, Sylvain; Lambert, Christophe; Lachambre, Sébastien.
The domestication of Haliotis tuberculata began recently. During this domestication process, abalone may acquire behavioural and physiological traits to become more adapted to their captive environment. These modifications could be the result of intentional selection on production traits, or of unintentional selection due to specific conditions experienced in the farm environment. In order to study this process, progenies of 3 different broodstock origins were studied. Wild abalone, farmed abalone selected for faster growth at 5% selection pressure and randomly selected farmed abalone were used as broodstock. Farmed abalone were the third generation of abalone resulting from systematic mating between wild and farmed broodstock (either males or females were...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00404/51546/52138.pdf

Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus
Provedor de dados: 5
Autores: Huvet, Arnaud; Fabioux, Caroline; Mccombie, Helen; Lapegue, Sylvie; Boudry, Pierre.
The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely inter-fertile under controlled conditions. However, hybridization in the wild had not been investigated, mainly due to the lack of nuclear diagnostic markers. In the present paper, we first estimated genetic differentiation between these 2 closely related taxa using 8 microsatellite markers. Interestingly, 5 markers displayed significant differences of allele size between taxa. The subsequent sequencing of alleles of one of these microsatellites showed several...
Tipo: Text
Palavras-chave: Allele size; Microsatellites; Genetic differentiation; Hybridization; Crassostrea gigas; Crassostrea angulata.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-3355.pdf

A "G" chromosome banding study of three cupped oyster species : Crassostrea gigas, Crassostrea angulata and Crassostrea virginica (Mollusca : Bivalvia)
Provedor de dados: 5
Autores: Leitao, Alexandra; Thiriot-quievreux, Catherine; Malheiro, Isabel; Boudry, Pierre.
The G-banding technique was performed on chromosomes from gill tissue of three cupped oyster species : Crassostrea gigas, Crassostrea angulata and Crassostrea virginica. Identification of the ten individual chromosome pairs was obtained. Comparative analysis of G-banded karyotypes of the three species showed that their banding patterns generally resembled each other, with chromosome pair 3 being similar in all three species. However, differences from one species to another were also observed. The G-banding pattern highlighted greater similarities between C. gigas and C. angulata than between these two species and C. virginica, thus providing an additional argument for genetic divergence between, these two evolutionary lineages. C. gigas and C. angulata...
Tipo: Text
Palavras-chave: Crassostrea virginica; Crassostrea angulata; Crassostrea gigas; G banding; Chromosome.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-2736.pdf

Programme 3 : Gestion durable des productions ostréicoles : les apports de la génétique. Convention 2000 RPC-A-530 Ifremer - Région Poitou-Charentes
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Heurtebise, Serge; Ledu, Christophe; Blouin, Frederic; Phelipot, Pascal; Gerard, Andre.
L'étude de la variabilité génétique de l' huître creuse Crassastrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 % de la production mondiale d' huîtres. Introduite en France au début des années 1970 pour remplacer l' huître portugaise Crassostrea angulata, c'est aujourd 'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n' a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la...
Tipo: Text
Palavras-chave: Génétique; Ressources génétiques; Différenciation génétique; Hybridation; Anomalies chromosomiques; Huîtres creuses; Crassostrea gigas; Crassostrea angulata.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15564/12955.pdf

Using a satellite DNA family for species identification of commercial oysters
Provedor de dados: 5
Autores: Lopez-flores, I.; De La Herran, R.; Garrido-ramos, M. A.; Boudry, Pierre; Ruiz-rejon, C.; Ruiz-rejon, M.
The cultivation of oyster presents some problems related with the identification and differentiation of species as Ostrea edulis (European flat oyster) with respect to Ostrea stentina (Provence oyster) or Crassostrea angulata (portuguese oyster). Crossostrea gigas (Pacific oyster). We have characterized a satellite DNA family and used it as a molecular marker for genetic differentiation between commercial and non-commercial oyster species present in Europe. This marker clearly supports a high degree of differentiation between O. edulis and O. stentina and conversely upholds the contention that C. gigas and C. angulata are the same species.
Tipo: Text
Palavras-chave: Satellite DNA; Ostres stentina; Ostrea edulis; Genetic; Crassostrea angulata; Crassostrea gigas; Oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3484.pdf

Response to divergent selection for resistance to summer mortality in Pacific oyster spat : genetic parameters and correlated responses with subsequent survival and growth
Provedor de dados: 5
Autores: Boudry, Pierre; Bedier, Edouard; Ernande, Bruno; Degremont, Lionel.
The multidisciplinary program "MOREST" aims to improve our understanding of causes of summer mortality in Crassostrea gigas juveniles in France and to reduce its impact on oyster production. As part of this program, four successive generations of families were bred between 2001 and 2005 in our research hatchery in La Tremblade. Survival and growth were recorded in the field in three sites along the French coast (Ronce, Rivière d'Auray and Baie des Veys). The first generation (44 full-sib families nested within 17 half-sib families) was tested during summer 2001 to assess to what extent genetic variability exists for spat survival.  An important genetic basis was found (estimated narrow-sense heritability among sites = 0.83 ± 0.40). In contrast, lower...
Tipo: Text
Palavras-chave: Survival; Growth; Genetic parameters; Summer mortality; Selection; Genetic; Crassostrea gigas; Pacific oysters.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3458.pdf

Caractérisation sur estran et en laboratoire des 3 séries produites en troisième génération : G3SD - G3C1 - G3C2
Provedor de dados: 5
Autores: Degremont, Lionel; Fortin, Adeline; Boudry, Pierre.
Dans le cadre du programme MOREST, une première génération d'huître creuse Crassostrea gigas (G1) a été réalisée en 2001 à partir d'un plan de croisement hiérarchisé par les mâles. La caractérisation sur estran de 44 familles de plein-frères réparties en 17 familles de demi-frères a permis d'estimer l'héritabilité de la survie des naissains en période estivale. La valeur obtenue (h2 = 0,81 ± 0,29) indique que la majorité de la variance phénotypique est d'origine génétique. A partir de familles G1 sélectionnées pour les performances de survies, une sélection divergente visant à améliorer ou diminuer la survie du naissain en période estivale a été réalisée en 2002 et constitue la seconde génération (G2). La sélection divergente comprend deux séries de...
Tipo: Text
Palavras-chave: Huître creuse; Crassostrea gigas; Génétique; Sélection; Estran.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00035/14668/11970.pdf

Microsatellites development in Ostrea edulis and Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Lapegue, Sylvie; Boudry, Pierre; Beaumont, Andy.
The European flat oyster Ostrea edulis and the blue mussel Mytilus edulis are both very valuable commercial species across Europe. Despite their economical importance, only a few microsatellite markers have so far been developed in those species. The aim of this study was to develop new microsatellites for O. edulis and M. edulis that could be used for population genetics studies, genetic variability assessment of stocks, parentage analysis or genetic and QTL mapping studies. For Ostrea edulis, an enriched library was made by ecogenics GmbH (Zurich, Switzerland) from size selected genomic DNA ligated into SAULA/SAULB-linker and enriched by magnetic bead selection with biotin-labelled (GT)13 and (CT)13 oligonucleotide repeats (Gautschi et al. 2000a,b). Of...
Tipo: Text
Palavras-chave: M13 tail; Blue mussel; European flat oyster; Microsatellite.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4536.pdf