Consulta: subjectFacets:"Apoptosis"
Registros recuperados: 269
Data/hora: 11/06/2024 09:17:44

Potencial antileucémico in vitro de extractos de cuatro genotipos de Sechium spp. (Cucurbitaceae).
Provedor de dados: 32
Autores: Aguiñiga Sánchez, Itzen.
El género Sechium (Cucurbitaceae) está formado por 10 especies, entre ellas Sechuim compositum, Sechium chinantlense y Sechium edule (Jacq.) Sw.; solo esta última es fuente medicinal y de alimento, de la cual se ha generado el híbrido H-387-07-GISeM a partir de sus diversas variedades. En este trabajo se analiza el contenido de los principales grupos de metabolitos contenido en extractos de frutos de Sechuim compositum,  Sechium chinantlense y H-387-07-GISeM, la actividad antiproliferativa del extracto completo o fraccionado, así como el mecanismo de inhibición de la proliferación tanto en células leucémicas WEHI-3 como en células hematopoyéticas normales. En todas las especies estudiadas, los principales metabolitos detectados fueron terpenos y...
Palavras-chave: Cucurbitaceae; Antiproliferativo; Apoptosis; Fitoquímica; Antiproliferative; Phytochemical; Botánica; Maestría.
Ano: 2013
URL: http://hdl.handle.net/10521/1968

Anticancer and apoptosis-inducing activities of microbial metabolites
Provedor de dados: 69
Autores: Phonnok,Sirinet; Uthaisang-Tanechpongtamb,Wanlaya; Wongsatayanon,Benjamas Thanomsub.
The problems of systemic toxicity and drug resistance in cancer chemotherapy urge the continuing discovery of new anticancer agents. We explored the specific anticancer activity from microbial metabolites to find new lead compound. 394 microbial extracts were evaluated on anti-proliferative activity against 4 cancer cell lines using MTT assay. Of these, 20 samples showed varying degree of cytotoxicity but specifically to the cancer cell lines since the growth of normal cells was not significantly inhibited by 1 mg/ml of each cell extracts. The 4 most potent extracts exhibited strongest growth inhibition to each cancer cell type were selected for further studied. Cell morphological changes such as cell shrinkage, lose of surface contact and blebbing were...
Tipo: Journal article
Palavras-chave: Anticancer; Apoptosis; Bioactive compound; Caspase; Microbial product.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500001

New stereoisomeric derivatives of jasmonic acid generated by biotransformation with the fungus Gibberella fujikuroi affect the viability of human cancer cells
Provedor de dados: 69
Autores: Carvajal,Marcela; Espinoza,Luis; Caggia,Silvia; Cardile,Venera; Garbarino,Juan A; Peña-Cortés,Hugo; Russo,Alessandra.
Background: Several studies have shown that (-)-Jasmonic acid, (+)-7-iso-Jasmonic acid and its methyl ester, methyl jasmonate, have anti-cancer activity in vitro and in vivo, exhibiting selective cytotoxicity towards cancer cells. The degree of activity of these molecules is strongly related to their stereochemistry. The biotransformation of known compounds, natural or synthesized, related to interesting biological activities, generates new molecules displaying new improved properties compared with the original ones, increasing its value and providing new more effective products. Therefore, based on the above rationales and observations, in this work a biotransformation protocol to modify the chemical structure of the plant hormone jasmonic acid by using...
Tipo: Journal article
Palavras-chave: Apoptosis; Biotransformations; Cancer cells; Cell vitality; Gibberella fujikuroi; Jasmonic acid.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200007

Using Bcl-xL anti-apoptotic protein for altering target cell apoptosis
Provedor de dados: 69
Autores: Iz,Sultan Gülçe; Çalimlioglu,Beste; Gürhan,Saime Ismet Deliloglu.
Background: Altering target cell apoptosis is one of the challenging ideas of biotechnological applications. There are several applications of over expressing Bcl-xL anti-apoptotic protein from recombinant protein production to DNA vaccination strategies. The aim of the present study is to evaluate the anti-apoptotic efficacy of Bcl-xL expressing dual promoter plasmid system as a candidate to be used for recombinant protein production and DNA vaccination approaches. For this purpose, Bcl-xL anti-apoptotic protein gene was inserted in a dual expressing vector system in frame with EGFP (enhanced green fluorescence protein) after IRES (internal ribosomal site). The plasmid has a multiple cloning site after CMV (cytomegalovirus promoter) left empty to be...
Tipo: Journal article
Palavras-chave: Animal cell biotechnology; Apoptosis; Bcl-xL anti-apoptotic protein.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500002

Electroacupuncture inhibits apoptosis in annulus fibrosis cells through suppression of the mitochondria-dependent pathway in a rat model of cervical intervertebral disc degradation
Provedor de dados: 74
Autores: Liao,Jun; Ke,Meigui; Xu,Teng; Lin,Lili.
The purpose of this study was to investigate whether treatment with electroacupuncture (EA) inhibited mitochondria-dependent apoptosis in annulus fibrosis (AF) cells in a rat model of cervical intervertebral disc degradation induced by unbalanced dynamic and static forces. Forty Sprague-Dawley rats were used in this study, of which 30 underwent surgery to induce cervical intervertebral disc degradation, 10 rats received EA at acupoints Dazhui (DU 14) and Shousanli (LI 10). TUNEL staining was measured to assess apoptosis in AF cells, immunohistochemistry was used to examine Bcl-2 and Bax expression, colorimetric assays were used to determine caspase 9 and caspase 3 activities and RT-PCR and western blotting were used to assess the mRNA and protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annulus fibrosis cells; Apoptosis; Electroacupuncture; Mitochondria.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400021

Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)
Provedor de dados: 74
Autores: Rizo,Walace Fraga; Ferreira,Luis Eduardo; Colnaghi,Vanessa; Martins,Juliana Simões; Franchi,Leonardo Pereira; Takahashi,Catarina Satie; Beleboni,Rene Oliveira; Marins,Mozart; Pereira,Paulo Sérgio; Fachin,Ana Lúcia.
Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 3T3; Apoptosis; Comet assay; Indole alkaloid; LDH.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100015

Role of the MAPK pathway in human lung epithelial-like A549 cells apoptosis induced by paraquat
Provedor de dados: 74
Autores: Sun,Da-Zhuang; Song,Chun-Qing; Xu,Yong-Min; Dong,Xue-Song.
Abstract This study aims to investigate the value of mitogen-activated protein kinases (MAPKs) for paraquat (PQ)-induced apoptosis in human lung epithelial-like A549 cells and the specific mechanism. A549 cell apoptosis were induced by PQ. These cells were divided into six groups: control group (cells were cultured in RPMI-1640 medium); SP600125 group (cells were preconditioned with SP600125); SB203580 group (cells were preconditioned with SB203580); PQ group (cells were treated with PQ); SP600125+PQ group (cells were preconditioned with SP600125 following PQ); SB203580+PQ group (cells were preconditioned with SB203580 following PQ). The cell survival rate, apoptosis rate, and activities of caspase-3 and -9 were detected. When compared with the control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paraquat; A549 cells; MAPKs; Mitochondria; Apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400701

LNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action In Hepatoma Cells
Provedor de dados: 74
Autores: Zanelatto,Leonardo Campos; Silva,Patrícia Benites Gonçalves da; Sartori,Daniele; Panis,Carolina; Lepri,Sandra; Fátima,Ângelo de; Mantovani,Mário Sérgio.
Abstract The identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thalidomide analogues; Antiproliferative activity; Cytotoxicity; Free radicals; Apoptosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200270

Targeting STAT-3 signaling pathway in cancer for development of novel drugs: Advancements and challenges
Provedor de dados: 74
Autores: Arshad,Sundas; Naveed,Muhammad; Ullia,Mahad; Javed,Khadija; Butt,Ayesha; Khawar,Masooma; Amjad,Fazeeha.
Abstract Signal transducers and activators of transcription 3 (STAT-3) is a transcription factor that regulates the gene expression of several target genes. These factors are activated by the binding of cytokines and growth factors with STAT-3 specific receptors on cell membrane. Few years ago, STAT-3 was considered an acute phase response element having several cellular functions such as inflammation, cell survival, invasion, metastasis and proliferation, genetic alteration, and angiogenesis. STAT-3 is activated by several types of inflammatory cytokines, carcinogens, viruses, growth factors, and oncogenes. Thus, the STAT3 pathway is a potential target for cancer therapeutics. Abnormal STAT-3 activity in tumor development and cellular transformation can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: STAT-3; DNA binding domain; Apoptosis; Drug discovery STAT-3 inhibitors.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100101

Toxicity and genotoxicity in Astyanax bimaculatus (Characidae) induced by microcystins from a bloom of Microcystis spp
Provedor de dados: 74
Autores: Silva,Ricardo Rocha Pavan da; Pires Junior,Osmindo Rodrigues; Grisolia,Cesar Koppe.
Studies of genotoxicity in fish caused by cyanobacterial microcystins can be useful both in determining the sensitivity of native species, as well as comparing exposure routes. The genotoxicity caused by the microcystins LR and LA from a bloom collected in a eutrophic lake, was revealed in the fish Astyanax bimaculatus, a native species from South America. LC50 (72 h) was determined as 242.81 µg L-1 and LD50 (72 h) as 49.19 µg kg-1 bw. There was a significant increase of DNA damage in peripheral erythrocytes, following intraperitoneal injection (ip) with tested concentrations of 24.58 µg kg-1 bw and 36.88 µg kg-1 bw, as well as through body exposure to a concentration of 103.72 µg L-1. Micronucleus (MN) induction was observed after ip injections of 24.58...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcystin; Comet assay; Fish micronucleus; Necrosis; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400023

Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line
Provedor de dados: 74
Autores: Carminati,Patricia Oliveira; Mello,Stephano Spano; Fachin,Ana Lucia; Junta,Cristina Moraes; Sandrin-Garcia,Paula; Carlotti,Carlos Gilberto; Donadi,Eduardo Antonio; Passos,Geraldo Aleixo Silva; Sakamoto-Hojo,Elza Tiemi.
Gliomas are the most common tumors in the central nervous system, the average survival time of patients with glioblastoma multiforme being about 1 year from diagnosis, in spite of harsh therapy. Aiming to study the transcriptional profiles displayed by glioma cells undergoing cisplatin treatment, gene expression analysis was performed by the cDNA microarray method. Cell survival and apoptosis induction following treatment were also evaluated. Drug concentrations of 12.5 to 300 μM caused a pronounced reduction in cell survival rates five days after treatment, whereas concentrations higher than 25 μM were effective in reducing the survival rates to ~1%. However, the maximum apoptosis frequency was 20.4% for 25 μM cisplatin in cells analyzed at 72 h,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cisplatin; Gene expression; Glioma.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100027

Cardanol: toxicogenetic assessment and its effects when combined with cyclophosphamide
Provedor de dados: 74
Autores: Schneider,Beatriz Ursinos Catelan; Meza,Alisson; Beatriz,Adilson; Pesarini,João Renato; Carvalho,Pamela Castilho de; Mauro,Mariana de Oliveira; Karaziack,Caroline Bilhar; Cunha-Laura,Andréa Luiza; Monreal,Antônio Carlos Duenhas; Matuo,Renata; Lima,Dênis Pires de; Oliveira,Rodrigo Juliano.
Abstract Cardanol is an effective antioxidant and is a compound with antimutagenic and antitumoral activity. Here, we evaluated the genotoxic and mutagenic potential of saturated side chain cardanol and its effects in combination with cyclophosphamide in preventing DNA damage, apoptosis, and immunomodulation. Swiss mice were treated with cardanol (2.5, 5 and 10 mg/kg) alone or in combination with cyclophosphamide (100 mg/kg). The results showed that cardanol is an effective chemopreventive compound, with damage reduction percentages that ranged from 18.9 to 31.76% in the comet assay and from 45 to 97% in the micronucleus assay. Moreover, cardanol has the ability to reduce the frequency of apoptosis induced by cyclophosphamide. The compound did not show...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic lipid; Antimutagenesis; Micronucleus; Comet assay; Apoptosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200279

Impact of the tissue factor pathway inhibitor gene on apoptosis in human vascular smooth muscle cells
Provedor de dados: 74
Autores: Dong,Xia; Song,Li-ping; Zhu,Dun-wan; Zhang,Hai-ling; Liu,Lan-xia; Leng,Xi-gang.
Tissue factor pathway inhibitor (TFPI) plays a vitally important role in the blood coagulation pathway. Recent studies indicated that TFPI induces apoptosis in vascular smooth-muscle cells (VSMCs) in animals. The present study investigated whether the TFPI gene could also induce apoptosis in human vascular smooth-muscle cells (hVSMCs). Such cells were isolated from human umbilical arteries and subsequently transfected with pIRES-TFPI plasmid (2 μg/mL). MTT assaying and cell counting were applied to measure cell viability and proliferation, RT-PCR was utilized to analyze TFPI gene expression in the cells. Apoptosis was analyzed by fluorescence activated cell sorting (FACS). Several key proteins involved in apoptosis were examined through Western blotting....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue factor pathway inhibitor; Vascular smooth muscle cells; Apoptosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100005

Chromosomal damage and apoptosis analysis in exfoliated oral epithelial cells from mouthwash and alcohol users
Provedor de dados: 74
Autores: Rocha,Rodrigo dos Santos; Meireles,José Roberto Cardoso; Cerqueira,Eneida de Moraes Marcílio.
Chromosomal damage and apoptosis were analyzed in users of mouthwash and/or alcoholic beverages, using the micronucleus test on exfoliated oral mucosa cells. Samples from four groups of 20 individuals each were analyzed: three exposed groups (EG1, EG2 and EG3) and a control group (CG). EG1 comprised mouthwash users; EG2 comprised drinkers, and EG3 users of both mouthwashes and alcoholic beverages. Cell material was collected by gently scraping the insides of the cheeks. Then the cells were fixed in a methanol/acetic acid (3:1) solution and stained and counterstained, respectively, with Schiff reactive and fast green. Endpoints were computed on 2,000 cells in a blind test. Statistical analysis showed that chromosomal damage and apoptosis were significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus; Apoptosis; Mouthwash; Alcoholic beverages.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500013

Crude alkaloid extract of Rhazya stricta inhibits cell growth and sensitizes human lung cancer cells to cisplatin through induction of apoptosis
Provedor de dados: 74
Autores: Elkady,Ayman I.
There is an urgent need to improve the clinical management of non-small cell lung cancer (NSCLC), one of the most frequent causes of cancer-related deaths in men and women worldwide. Rhazya stricta, an important medicinal plant used in traditional Oriental medicine, possesses anti-oxidant, anti-carcinogenic and free radical scavenging properties. This study was done to explore the potential anticancer activity of a crude alkaloid extract of R. stricta (CAERS) against the NSCLC line A549. CAERS markedly suppressed the growth of A549 cells and considerably enhanced the anti-proliferative potential of cisplatin. CAERS-mediated inhibition of A549 cell growth correlated with the induction of apoptosis that was accompanied by numerous morphological changes, DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Lung cancer; Medicinal plant; RT-PCR; Western blot.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100003

Apoptose et infections à virus de type herpès chez les bivalves marins : détection de séquences codant pour des protéines inhibitrices de l'apoptose
Provedor de dados: 5
Autores: Didier, Yohann.
Tipo: Text
Palavras-chave: PCR; Apoptosis; Marine bivalve; Herpes like virus.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/rapport-1768.pdf

Etude de nouvelles cibles moleculaires de cancer bronchopulmonaire non a petites cellules pharmacomodulées par des substances originales naturelles et synthetiques
Provedor de dados: 5
Autores: Moreau, Dimitri.
Still rare disease at the beginning of the 20th century, the lung cancer is at the origin of more than 6 % of the deaths in France currently. It occupies there the first rank of the deaths by cancer whereas France is regarded as a country at the average risk among the Western countries. The discovery of new anti-cancer treatments increasingly more effective and specific, is thus today of primary importance.  The validation of the capacity of a molecule to being able to inhibit the cancerous proliferation, passes by several stages of tests starting with the description of an activity at the cellular level. One of the strategies used for the discovery of new compounds antiproliferativs, passes by an investigation of the bioactiv potential of a great number...
Tipo: Text
Palavras-chave: Microalgae; Natural products; Apoptosis; Antiproliferativ; Gene expression; Regulatory RNA; Molecular targets; Bronchopulmonary cancer; Microalgues; Molécules naturelles; Apoptose; Antiprolifératif; Expression génique; ARN régulateur; Cibles moléculaires; Cancer bronchopulmonaire.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/these-2196.pdf

Concomitant herpes-like virus infections in hatchery-reared larvae and nursery-cultured spat Crassostrea gigas and Ostrea edulis
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Concomitant sporadic high mortalities were reported in France in May 1994 among batches of hatchery-reared larval Pacific oysters Crassostrea gigas and European flat oysters Ostrea edulis in 2 hatcheries, and in June and July 1994 among batches of cultured spat of both species in a shellfish nursery. Histological observation showed the presence of cellular abnormalities in moribund animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in infected larvae and spat of both oyster species. This is the first description of a herpes-like virus infection in larval O.. edulis. Viruses observed in diseased larvae and spat of both species are similar with respect to ultrastructure and morphogenesis, They were detected...
Tipo: Text
Palavras-chave: Apoptosis; Virus replication; Concomitant infection; Oyster mortality; Crassostrea gigas; Pacific oyster; Ostrea edulis; European flat oyster; Herpes like virus.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-820.pdf

Exposure to the Neurotoxic Dinoflagellate, Alexandrium catenella, Induces Apoptosis of the Hemocytes of the Oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Medhioub, Walid; Ramondenc, Simon; Vanhove, Audrey; Vergnes, Agnes; Masseret, Estelle; Savar, Veronique; Amzil, Zouher; Laabir, Mohamed; Rolland, Jean-luc.
This study assessed the apoptotic process occurring in the hemocytes of the Pacific oyster, Crassostrea gigas, exposed to Alexandrium catenella, a paralytic shellfish toxins (PSTs) producer. Oysters were experimentally exposed during 48 h to the toxic algae. PSTs accumulation, the expression of 12 key apoptotic-related genes, as well as the variation of the number of hemocytes in apoptosis was measured at time intervals during the experiment. Results show a significant increase of the number of hemocytes in apoptosis after 29 h of exposure. Two pro-apoptotic genes (Bax and Bax-like) implicated in the mitochondrial pathway were significantly upregulated at 21 h followed by the overexpression of two caspase executor genes (caspase-3 and caspase-7) at 29 h,...
Tipo: Text
Palavras-chave: Shellfish; Toxins; Apoptosis; Gene expression.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00176/28744/27213.pdf

Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf

OsHV-1 countermeasures to the Pacific oyster’s anti-viral response
Provedor de dados: 5
Autores: Green, Timothy J.; Rolland, Jean-luc; Vergnes, Agnes; Raftos, David; Montagnani, Caroline.
The host-pathogen interactions between the Pacific oyster (Crassostrea gigas) and Ostreid herpesvirus type 1 (OsHV-1) are poorly characterised. Herpesviruses are a group of large, DNA viruses that are known to encode gene products that subvert their host’s antiviral response. It is likely that OsHV-1 has also evolved similar strategies as its genome encodes genes with high homology to C. gigas inhibitors of apoptosis (IAPs) and an interferon-stimulated gene (termed CH25H). The first objective of this study was to simultaneously investigate the expression of C. gigas and OsHV-1 genes that share high sequence homology during an acute infection. Comparison of apoptosis-related genes revealed that components of the extrinsic apoptosis pathway (TNF) were...
Tipo: Text
Palavras-chave: Crassostrea; Anti-viral response; Apoptosis; Interferon-like; Poly I:C.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00279/39048/37610.pdf

Virus-like particles associated with brown muscle disease in Manila clam, Ruditapes philippinarum, in Arcachon Bay (France)
Provedor de dados: 5
Autores: Dang, C.; Gonzalez, P.; Mesmer-dudons, N.; Bonami, J-r; Caill-milly, Nathalie; De Montaudouin, X.
Recently, Manila clam, Ruditapes philippinarum, populations have suffered mortalities in Arcachon Bay (SW France). Mortality was associated with extensive lesions of the posterior adductor muscle, which become progressively brown and calcified. Ultrastructural observations by transmission electron microscopy revealed tissue degradation with necrotized muscle fibres and granulocytomas. Unenveloped virus-like particles (VLPs) were detected in muscle, granulocytic, epithelial and rectal cells. VLPs were abundant in the extracellular space, in the cytoplasm (free or enclosed in vesicles) and in the nucleoplasm of granulocytes. Nuclei and mitochondria of granulocytes displayed changes which suggested reactive oxygen species production and apoptosis induction....
Tipo: Text
Palavras-chave: Adductor muscle; Apoptosis; Brown muscle disease; Necrosis; Ruditapes philippinarum; Transmission electron microscopy.
Ano: 2009
URL: https://archimer.ifremer.fr/doc/00000/11130/7991.pdf

Antiproliferative Activity of Cyanophora paradoxa Pigments in Melanoma, Breast and Lung Cancer Cells
Provedor de dados: 5
Autores: Baudelet, Paul-hubert; Gagez, Anne-laure; Berard, Jean-baptiste; Juin, Camille; Bridiau, Nicolas; Kaas, Raymond; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent.
The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 mu g.mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 mu g.mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of...
Tipo: Text
Palavras-chave: Pigments; Microalgae; Cyanophora paradoxa; Melanoma; Cancer; Apoptosis; Zeaxanthin; Cryptoxanthin.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00177/28818/27289.pdf

Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts
Provedor de dados: 5
Autores: Pasquet, Virginie; Morisset, Perrine; Ihammouine, Said; Chepied, Amandine; Aumailley, Lucie; Berard, Jean-baptiste; Serive, Benoit; Kaas, Raymond; Lanneluc, Isabelle; Thiery, Valerie; Lafferriere, Mathieu; Piot, Jean-marie; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at...
Tipo: Text
Palavras-chave: Pigments; Microalgae; Dunaliella; Violaxanthin; Carotenoid; Cancer; Apoptosis.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15142/12575.pdf

Etude cellulaire et moléculaire de l’apoptose chez l’huître plate Ostrea edulis en réponse au parasite Bonamia ostreae
Provedor de dados: 5
Autores: Gervais, Ophelie.
The flat oyster, Ostrea edulis, is the European endemic oyster species. Its production has been reduced because of overfishing and diseases including bonamiosis. Massive mortalities observed on the Pacific cupped oyster these last years explained the wish of shellfish farmers to diversify their production and their revival of interest for this patrimonial oyster species. However, bonamiosis due to the protozoan, Bonamia ostreae, is still a major problem for the production of this species. Measures to control the disease are limited and require a better knowledge of the interactions between the flat oyster and the parasite. Previous studies have suggested the involvement of apoptosis in flat oyster defense mechanisms against B. ostreae. This mechanism is...
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Bonamia ostreae; Apoptose; Flat oyster; Ostrea edulis; Bonamia ostreae; Apoptosis.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00370/48142/48243.pdf

Induction of apoptosis by UV in the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle.
Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that...
Tipo: Text
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf

Mitochondria-targeted Triphenylamine Derivatives Activatable by Two-Photon Excitation for Triggering and Imaging Cell Apoptosis
Provedor de dados: 5
Autores: Chennoufi, Rahima; Bougherara, Houcine; Gagey-eilstein, Nathalie; Dumat, Blaise; Henry, Etienne; Subra, Frederic; Bury-mone, Stephanie; Mahuteau-betzer, Florence; Tauc, Patrick; Teulade-fichou, Marie-paule; Deprez, Eric.
Photodynamic therapy (PDT) leads to cell death by using a combination of a photosensitizer and an external light source for the production of lethal doses of reactive oxygen species (ROS). Since a major limitation of PDT is the poor penetration of UV-visible light in tissues, there is a strong need for organic compounds whose activation is compatible with near-infrared excitation. Triphenylamines (TPAs) are fluorescent compounds, recently shown to efficiently trigger cell death upon visible light irradiation (458 nm), however outside the so-called optical/therapeutic window. Here, we report that TPAs target cytosolic organelles of living cells, mainly mitochondria, triggering a fast apoptosis upon two-photon excitation, thanks to their large two-photon...
Tipo: Text
Palavras-chave: Apoptosis; Small molecules.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00320/43168/42726.pdf

A herpes-like virus infects a non-ostreid bivalve species: virus replication in Ruditapes philippinarum larvae
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile; Arzul, Isabelle.
Sporadic high mortalities were reported in June 1997 among hatchery-reared larval Manila clam Ruditapes philippinarum in a French commercial hatchery. Cellular abnormalities were observed in semi-thin sections in affected animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in larvae. This is the first description of a herpes-like virus infection in larval R. philippinarum, a non-ostreid bivalve species. Virus particles were similar to other herpes-like viruses described from different oyster species with respect to ultrastructure and morphogenesis. Electron microscopic examination also demonstrated cells with condensed chromatin and extensive perinuclear fragmentation of chromatin. Like viruses infecting oysters,...
Tipo: Text
Palavras-chave: Apoptosis; Virus replication; Mortality; Ruditapes philippinarum; Manila clam; Herpes like virus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-816.pdf

Deciphering the molecular adaptation of the king scallop (Pecten maximus) to heat stress using transcriptomics and proteomics
Provedor de dados: 5
Autores: Artigaud, Sebastien; Richard, Joelle; Thorne, Michael A. S.; Lavaud, Romain; Flye-sainte-marie, Jonathan; Jean, Fred; Peck, Lloyd S.; Clark, Melody S.; Pichereau, Vianney.
Background: The capacity of marine species to survive chronic heat stress underpins their ability to survive warming oceans as a result of climate change. In this study RNA-Seq and 2-DE proteomics were employed to decipher the molecular response of the sub-tidal bivalve Pecten maximus, to elevated temperatures. Results: Individuals were maintained at three different temperatures (15, 21 and 25 degrees C) for 56 days, representing control conditions, maximum environmental temperature and extreme warming, with individuals sampled at seven time points. The scallops thrived at 21 degrees C, but suffered a reduction in condition at 25 degrees C. RNA-Seq analyses produced 26,064 assembled contigs, of which 531 were differentially expressed, with putative...
Tipo: Text
Palavras-chave: Marine biology; Metabolism; DNA repair; Transcription regulation; Apoptosis; Energy reserves.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00301/41173/40360.pdf

Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae
Provedor de dados: 5
Autores: Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle.
The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses....
Tipo: Text
Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf

Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes
Provedor de dados: 77
Autores: Ghafari,Soraya; Asadi,Ebrahim; Shabani,Ronak; Golalipour,Mohammad Jafar.
Gestational diabetes mellitus (GDM) defined as impaired glucose tolerance affects approximately 6 % of all pregnant women who have never before had diabetes, but who do have high blood glucose levels during pregnancy. This study was done to evaluate the apoptosis in the neuronal cells in the CA1, CA2 and CA3 subfields of hippocampus and dentate gyrus in offspring of gestational diabetes at the 7, 21 and 28 d in postnatal rats. Thirty Wistar rat dams were randomly allocated in control and diabetic group. Dams in diabetic group were received 40 mg/kg/BW of streptozotocin at the first day of gestation and control groups received an equivalent volume normal saline injection intraperitoneally (IP). Six offspring of GDM and control dams, at the 7, 21, 28...
Tipo: Journal article
Palavras-chave: Gestational diabetes; Hippocampus; Neuron; Apoptosis; TUNEL Assay; Dentate gyrus; Rat.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100029

Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring
Provedor de dados: 77
Autores: Nazari,Zahra; Nabiuni,Mohammad; Ghaffari,Soraya; Saeidi,Mohsen; Shahriyari,Alireza; Golalipour,Mohammad Jafar.
Several studies indicated that pancreatic ß-cell death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the ß-cells in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated in control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and controls at the age of 16 weeks were sacrificed and pancreases harvested and fixed. The number of ß-cells and were counted by Gomori's method staining. Also, apoptosis in pancreas tissue of diabetic and control offspring was detected by TUNEL assay. Results showed a significant...
Tipo: Journal article
Palavras-chave: Gestational diabetes mellitus; Pancreatic islets (Langerhans); SS-cell; Apoptosis; Rat offspring.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100003

Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks
Provedor de dados: 77
Autores: Sun,PengPeng; Luo,You; Wu,Xin Tong; Ansari,Abdur Rahman; Wang,Jing; Yang,KeLi; Xiao,Ke; Peng,KeMei.
Boron is an essential trace element which plays an important role in process of metabolism and the function of the tissues. However, the effects of boron on the intestinal cells in African ostrich chicks are poorly reported. Therefore, this study was designed to investigate the role of boron on proliferation and apoptosis of the intestinal cells. A total of 36, ten day-old ostrich chicks were randomly divided into six groups and fed on the same basal diet supplemented with 0, 40, 80, 160, 320 and 640 mg/L boric acid in drinking water for 80 days. Proliferatingcell nuclearantigen (PCNA) wasused to test the proliferation indexof intestine in different group byimmunohistochemicalstaining (IHC). Apoptoticcellsofintestinewere detectedbyDutp-biotin nick end...
Tipo: Journal article
Palavras-chave: Apoptosis; Boron; Intestine; Ostrich chicks; Proliferation.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300002

Angiogenesis and Tumor Progression Inhibition of Cyclooxygenase-2 Selective Inhibitor Celecoxib Associated with Poly (lactic-co-glycolic acid) in Tumor Cell Line Resistant to Chemotherapy
Provedor de dados: 77
Autores: Roa,Ignacio; Cantín,Mario; Vilos,Cristian; Rosas,Carlos; Lemus,David.
Although, antineoplastic therapies have now been developed reduction of tumor progression,itis necessarytofind new therapeutic alternatives to suppress angiogenesis.Thus celecoxib (Cx) has been used for its antiangiogenic action in combination with certain polymeric compounds such as poly (lactic co-glycolic acid) (PLGA) acid, which help to improve the bioavailability and avoid effects of long drug administrations. For this purpose we used a murine tumor modelinduced by mammary adenocarcinoma cells resistant to chemotherapy (TA3-MTXR). CX/PLGA inhibits the microvascular density, VEGF expression and cell proliferationinaddition to increased apoptosis (P &lt;0.0001). Cx reduces tumor progression in a concentration of 1000 ppm associated with PLGA, reducing...
Tipo: Journal article
Palavras-chave: Angiogenesis; Cancer; Celecoxib; PLGA; Apoptosis; VEGF; CellProliferation.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200055

Apoptosis in Denervated Skeletal Muscle
Provedor de dados: 77
Autores: Davatz,Giovanna Castilho; Andreo,Jesus Carlos; Rodrigues,Antonio de Castro; Rosa Júnior,Geraldo Marco; Moraes,Luiz Henrique Rapucci.
The apoptosis phenomenon happens in normal tissues and in many pathological conditions as described by several researchers. Most studied in the 70s, this phenomenon remained almost forgotten in the 90s, when, with the advent of more advanced techniques, returned to laboratories. Thus, electronic microscopy, Comet Assays and histochemistry techniques, among others, were utilized in an effort to elucidate in a thorough way, the mechanisms that lead the cell to "suicide". No one can deny the importance of the knowledge of such phenomenon, so as to control its induction or blocking in the treatment of pathologies such as cancer. In this work, the apoptosis study was aimed at denervated muscles in different periods. Furthermore, two types of skeletal striated...
Tipo: Journal article
Palavras-chave: Skeletal muscle; Denervation; Apoptosis; Rat.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300009

Prenatal Water Deprivation Induces Apoptosis in Sexual Dimorphic Nucleus of the Brain of Male New Born Sprague-Dawley Rats
Provedor de dados: 77
Autores: Chehreie,Shima; Sadri,Soheil; Khazaei,Mozafar; Ghanbari,Ali; Ayubian,Mahmood; Amiri,Sara.
Considering the size of some nuclei and area, sex hormones control the sexual development of the brain. The sexual development of the brain can also be influenced by environmental stress. This study aimed to clear the effect of prenatal water deprivation on the development of sexual dimorphic nucleus (SDN) of the brain. In this research, pregnant rats were divided into two groups (control and treated). For the treated animals, water was removed from the ewes for 48 h at the end of third trimester of gestation (19-21 days). TUNEL staining was used for detection of apoptosis in paraffin embedded diencephalon selected sections. The ratio of apoptotic cells to non- apoptotic ones was calculated as apoptotic index. Differences of apoptotic index and serum...
Tipo: Journal article
Palavras-chave: Apoptosis; Rat; SDN nucleus; Stress; TUNEL.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200032

Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue
Provedor de dados: 77
Autores: Alvarez,Marco; Urbina,Gidalia; Müller,Claudia; Perdomo,Lourdes.
The aim of this study was to evaluate the acute cell injury of excretion products present in culture filtrate from Shigella dysenteriae in both whole lower limb of chick embryo ex vivo and myoblasts cells developed in hanging-drop cultures in vitro. Three controls were defined: a) Tyrode's solution b) brain-heart broth infusion (CCC) and c) supernatant not toxigenic of E.coli 0157:H7. Shigella dysenteriae were culture for 24 hours and the excretion products were obtained after centrifugation of the culture. After lh of treatment, the morphologic changes in limbs treated with raw filtrate were evaluated through histopathological examination of sections stained with hematoxylin-eosin (H&amp;E-stain) and Gomori's trichrome by image analysis techniques....
Tipo: Journal article
Palavras-chave: Shigella dysenteriae; Shiga toxin; Chick embryo; Muscle tissue; Apoptosis.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300024

MICE TESTICULAR DAMAGE ELICITED BY MALATHION
Provedor de dados: 77
Autores: Bustos-Obregón,Eduardo; González-Hormazábal,Patricio.
Organophosphorous pesticides are a healh problem worldwide, mostly for agroworkers, which are around 2600 millions of person in the year 2001. Little is known about male reproductive damage elicited by these chemicals. The acute effects of malathion (single intraperitoneal injection), 1/12 the LD50 to mice were analyzed at 1, 8, 16, 35 and 40 days after injection, in terms of spermatogenic cell proliferation and apoptosis and of Sertoli cell compromise as revealed by immunocytochemical detection of CK-18 (cytokeratin). The micronuclei test was done to assess for genotoxic activity of the agropesticide. The results showed decreased germ cell number followed by compensatory spermatogonial proliferation by 16 days (as shown by thymidine-H3 testicular uptake),...
Tipo: Journal article
Palavras-chave: Spermatogenesis; Organophosphorous agropesticides; Proliferation; Apoptosis.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000200010

El Extracto Acuoso de Ruta graveolens del Norte de México Causa Apoptosis y Muestra Efecto Antiproliferativo sobre el Hígado de Rata Wistar: Evidencia Morfológica
Provedor de dados: 77
Autores: Soto-Domínguez,Adolfo; Serrano-Gallardo,Luis B; García-Garza,Rubén; Ruíz-Flores,Pablo; Morán-Martínez,Javier; Nava-Hernández,Martha P; Martínez-Pérez,Edith F.
Ruta graveolens es una planta nativa del Mediterráneo Oriental y del área Sur Occidental de Asia, de esta planta se han aislado más de 120 compuestos químicos. En un estudio previo en nuestro laboratorio se observó que un extracto acuoso de R. graveolens causó necrosis y alteraciones morfológicas sugestivas de apoptosis sobre el hígado de rata Wistar. El objetivo del presente estudio, fue evaluar la inducción de apoptosis y el posible efecto antiproliferativo in vivo de un extracto acuso de R. graveolens del norte de México, mediante métodos inmunohistoquímicos. Se utilizaron 25 ratas Wistar y se dividieron en 5 grupos (n=5). El grupo 1 correspondió al grupo control negativo, el grupo 2 o control positivo se trató con 100 mg de dexametasona/kg/día. Los...
Tipo: Journal article
Palavras-chave: Ruta graveolens; Apoptosis; Efecto antiproliferativo.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400031

Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
Provedor de dados: 77
Autores: Ili,Carmen G; Brebi,Priscilla; Garcia,Patricia; Leal,Pamela; Lopez,Jaime; Tapia,Oscar; Letelier,Pablo; Weber,Helga; Castillo,Jonathan; Roa,Juan C.
Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected...
Tipo: Journal article
Palavras-chave: Apoptosis; Proliferation; Cervical cancer; SiRNA; C-FLIPL.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036

Histological Changes in Ovaries of Mice Exposed to Butea monosperma: Preliminary Study
Provedor de dados: 77
Autores: Gupta,Neelam; Singh,Gajendra; Singh,S. M; Reddy,K. R. C.
In Ayurvedic practice Butea monosperma (Palash) is in clinical use for hundreds of years as a contraceptive. Seeds of Butea monosperma are also used as an anthelmitic (Ansani et al., 1979) and antimicrobial (Avirutnant &amp;amp; Pongpan, 1983). Butea monosperma (Fabaceae family) locally known as Palash (Dhak) if given for 3 consecutive days acts as an antifertility agent for which it has been is traditionally used since time immemorial. The objective of the present study was to search the effect of Butea monosperma seeds on the ovary of mice. Observations in the present study were massive degeneration of ova in almost all the follicles, irrespective of the stage of their development. The ova from treated animals showed different stages of necrotic process....
Tipo: Journal article
Palavras-chave: Palash; Butea monosperma; Apoptosis; Disintegration; Follicles.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400051

Apoptosis Durante el Desarrollo Embrionario de la Retina de Tortuga (Trachemys scripta elegans)
Provedor de dados: 77
Autores: Segovia,Y.
La apoptosis o muerte celular programada es un proceso que ocurre durante el desarrollo del sistema nervioso. El objetivo de este estudio fue observar los patrones de apoptosis que se producen durante el desarrollo embrionario de la retina, desde el estadio S16 al momento del nacimiento, mediante miscoscopía óptica y electrónica. Se utilizaron retinas de embriones de tortuga. Nuestros datos muestran que los primeros signos de apoptosis comienzan en el estadio S16, en la capa nuclear interna y alcanzan su máxima densidad tanto en la capa nuclear interna como en la capa de células ganglionares en S20, para extinguirse, prácticamente, en el momento del nacimiento. Por otra parte, la apoptosis sigue un gradiente centro-periferia.
Tipo: Journal article
Palavras-chave: Trachemys scripta elegans; Apoptosis; Retina; Tortuga.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100020

Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approach
Provedor de dados: 77
Autores: da Silva,Jairo Pinheiro; de Carvalho,Camila Albuquerque Mello; Romualdo,Andressa Rodrigues; Schiavoni,Vagner Sarraipo; Barbosa,Marcello Henrique Nogueira; de Moraes,Marcella Suélen Torrecilla; Tirapelli,Daniela Petri Cunha; Fazan,Valéria de Paula Sassoli; Thomazini,José Antônio; Tirapelli,Luis Fernando.
The aim of this study is evaluate the efficacy of 904 nm laser diode in bone regeneration in the bone defect in diabetic rats. Six groups of 10 male Wistar rats and 2 mm bone defects drilled on the left and right tibia were used. The diabetic animals were treated with streptozotocin (40 mg/kg, i.v.). We compared the diode laser doses of treatment of bone defects 50 w ­ 4 J/cm and 100 w ­ 4J/. The right tibia was used for immunohistochemical analysis with the apoptosis markers XIAP and Caspase-3 and the left tibia was submitted to computer tomography (CT). Caspase-3 marker showed greater amount of apoptosis in all the untreated groups compared to both laser treatments. There was no statistical significance for XIAP marker. CT scan showed improvement of bone...
Tipo: Journal article
Palavras-chave: Bone defect; Diabetes mellitus; Laser therapy; Regeneration; Apoptosis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100029

The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma
Provedor de dados: 77
Autores: Hongfu,Liu; Luping,Zhang; Shaojie,Chen; Zengxian,Wang; Fei,Huang; Dong,Wang.
This study aimed to observe the regularity of liver cell apoptosis and expression of apoptosis related gene Bcl-2 and Bax in the induced cancer, and explore the relationship between apoptosis and the development of liver cancer. In this study, 84 rats were used, 72 rats as experimental group induced by drinking water containing DEN, 12 rats as control group (CG). After laparotomy, the form, color, texture of the liver and metastatic tumor in both control and experimental groups were observed and recorded. The metastatic tumor and the liver tissue were sectioned and stained with hematoxylin-eosin staining to demonstrate the characteristic in morphological changes, the Hoechst 33342 fluorescent staining was applied to show and count the rate of apoptotic...
Tipo: Journal article
Palavras-chave: Primary hepatic carcinoma; Apoptosis; Bcl-2; Bax; Rats.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400032

Death-Associated Protein Kinase is Underexpressed in High-Grade Oral Squamous Cell Carcinoma
Provedor de dados: 77
Autores: Meneses,Ismário Silva de; Souza,Rafael Reis de; Jeraldo,Verónica de Lourdes Sierpe; Cavalcante,Danielle Rodrigues Ribeiro; Reis,Francisco Prado; Albuquerque Júnior,Ricardo Luiz Cavalcanti de.
An immunohistochemical analysis of 40 cases of oral squamous cell carcinoma was performed to evaluate the relationship between the expression pattern of death-associated protein kinase (DAPk) positive cells with the histological malignancy grading of these lesions. According to our results, eleven cases (27.5%) were high-grade malignancy tumours and 29 (72.5%) were low-grade ones. We found that 92.86% of the low-grade tumours were positive to anti-DAP kinase antibody whereas only 7.14% of the high-grade tumours presented positivity, and this difference was statistically significant (p&lt;0.01). Sixteen (55.2%) of the low-grade carcinomas exhibited moderate immunoreactivity whereas ten cases (34.5%) showed weak staining and three cases (10.3%) were negative...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; DAP kinase; Apoptosis; Squamous cell carcinoma.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200044

AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus
Provedor de dados: 77
Autores: Bustos-Obregón,Eduardo; Esponda,Pedro.
Apoptosis has been largely analyzed in the testis. Nevertheless, the epididymis has been scarcely studied. We analyzed the number of apoptotic cells in the different regions (caput, corpus and cauda) of the epididymis of the South American rodent Octodon degus both young and senile. Apoptosis was identified using the TUNEL method which detects in situ DNA fragmentation. Apoptosis was detected in the principal cells of the epididymal epithelium. The caput epididymis was the region more affected. The caput of young animals showed that 0.32/1000 cells were apoptotic in contrast to 5.1/1000 of senile animals. Also in the cauda epididymis apoptosis is increased with age, appearing 0.14/1000 and 3.9/1000 in young and senile animals, respectively. On the other...
Tipo: Journal article
Palavras-chave: Androgens; Apoptosis; Epididymis; Ageing; Heat Shock Proteins.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000100005

Excretion Product of Shigella dysenteriae (SdyEP) Induced Cell Death in Early Larval Stage of Zebrafish (Danio rerio): Acridine Orange and Ethidium Bromide (AO/EB) in vivo Staining
Provedor de dados: 77
Autores: Alvarez,M; Urbina,G; Perdomo,L.
In the field of studies of acute toxicity induced by bacterial agents, Shiga toxins have been relevant due to the severity of the extra-intestinal diseases they cause. Numerous studies have shown that Shiga toxin induced apoptosis in different cell types; however, this important process has been little studied in vivo experimental models. In this study, the effects of excretion products of Shigella dysenteriae, in which Shiga toxin is present, were investigated on early larval stages of Zebrafish, an animal model with many advantages over other in vivo experimental models traditionally used. Both the collection of eggs and larvae of Zebrafish, and the product from excretion from Shigella dysenteriae (SdyEP) were performed according to laboratory standards....
Tipo: Journal article
Palavras-chave: Zebrafish (Danio rerio); Shigella dysenteriae; Apoptosis; Acridine Orange; Ethidium Bromide.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100014

Malathion Affects Spermatogenic Proliferation in Mouse
Provedor de dados: 77
Autores: Penna-Videau,S; Bustos-Obregón,E; Cermeño-Vivas,J. R; Chirino,D.
The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermatogenesis. For the experiments, male albino mice of strain NMRI-IVIC, weighing between 30-40 g were used, and divided into control and experimental groups of 5 each. The animals of the experimental groups were injected with a single dose of MP: 241mg/kg weight (1/12 LD 50 ) resuspended in 0.9% saline, intraperitoneally. Animals were sacrificed at 8.3, 16.6 and 33.2 days post-injection (first, second and third spermatogenic cycles). Testicular samples were...
Tipo: Journal article
Palavras-chave: Malathion; Testis; Mouse; Apoptosis; Morphology; P53; Proliferation.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400023

Quercetin Inhibits Left Ventricular Dysfunction Induced by Chronic Stress in Rats
Provedor de dados: 77
Autores: Bin-Jaliah,Ismaeel.
Complications of chronic stress including cardiovascular disease are among the common public health problems that affect the lives of millions of people around the globe. We sought to determine whether the anti-oxidant and anti-apoptotic agent, quercetin can inhibit chronic stress-induced left ventricular dysfunction (LVD). Chronic unpredictable stress (CUS) was induced in rats using a variety of stressors in the presence and absence of quercetin (50 mg/kg body weight/day). Harvested tissues from the left ventricles (LV) of these animals were examined using basic histological staining. In addition, LV tissue homogenates were assayed for markers of oxidative and anti-oxidative stress that are known to be modulated in cardiac dysfunction. Furthermore, LV...
Tipo: Journal article
Palavras-chave: Quercetin; Chronic stress; Heart failure; Left ventricular dysfunction; Antioxidant; Apoptosis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200044

Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis
Provedor de dados: 77
Autores: Novais,P. C; de Paula,A. A. P; Reis,R. B; Barione,D. F; Franqueiro,A. G; Rodrigues Jr,A. A; Lizarte,F. S. N; de Carvalho,C. A. M; Tirapelli,D. P. C; Peria,F. M; Saggioro,F. P; Tucci Jr,S; Cologna,A. J; Martins,A. C. P; Tirapelli,L. F.
The aim was to analyze the protein expression of apoptotic genes caspase-3, caspase-8 and bcl-2 with the immunohistochemistry technique, correlating with tumor grade (I, II and III) and with the patient survival in order to understand the basic mechanism of tumoral transformation. The immunohistochemistry reactions on 50 samples of squamous cell carcinoma were carried out with the avidin-biotin immunoperoxidase method and antigen recovery. The analyses were made using the graduation method "in crosses" (0 to 4 crosses - no stain to more than 75% of positives cells) and in categories (low, intermediate, high) of the cytoplasm immunoreactivity of the epidermoid penile carcinoma cells. It was observed a statistically significant difference when the expression...
Tipo: Journal article
Palavras-chave: Bcl-2; Caspase-3; Caspase-8; Penile cancer; Apoptosis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300043

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against Cisplatin­Induced Apoptosis on Testis in Mice
Provedor de dados: 77
Autores: Keshtmand,Zahra; Ghanbari,Ali; Khazaei,Mozafar; Rabzia,Arezou.
Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris (TT) has been used as an aphrodisiac. The present study amid to investigate protective effect of TT hydroalcoholic extract against cisplatin-induced apoptosis on testis in mice. Male adult mice (n=30) were divided into control and 4 experimental groups (n=6). Control group received saline, first experimental group received cisplatin (5.5 mg/kg) and other three experimental group received cisplatin (5.5 mg/kg) and different doses of hydroalcoholic extact of TT (100, 300 and 500 mg/kg/i.p) respectively. Day after the last injection, histopathology and histomorphic analysis...
Tipo: Journal article
Palavras-chave: Cisplatin; Tribulus terrestris; Apoptosis; TUNEL.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100044

Carbonated Water and Necro-Apoptotic Cell Death in Chicken Embrio Low Limb
Provedor de dados: 77
Autores: Álvarez,Marco; Perdomo,Lourdes.
Carbonated water is a fundamental part of many drinks and its effects have been studied in many pathological situations. However, cells and tissue damage as a consequence of carbonated water has not been the subject of extensive research. We assessed the short-term effects of soda on in vitro Hanging-drop culture of myoblasts and ex vivo lower limb of 8-day-old chicken embryo skeletal muscle tissue. Several groupswerendesigned: a) Control (Con-tyr), b) Carbonated water (Car), c) Coffe (Caf), and d) Cola beverage (Glu). The samples were observed with light microscopy and digital imaging analysis was performed. The ultra-structure of control and treated tissue were observed with electron microscopy. Immunohistochemistry techniques, such as terminal...
Tipo: Journal article
Palavras-chave: Soda Club; Chicken embryo; Low limb; Apoptosis; Necrosis.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200006

Increase in Apoptosis and of the Stress Protein HSP70 in the Mouse Epididymis Produced by the Antiandrogen Flutamide
Provedor de dados: 77
Autores: Bustos Obregón,Eduardo; Esponda,Pedro.
The general aim of this paper was to characterize some changes induced by androgen receptors blockage in the epithelial cells of the mouse epididymis. The antiandrogen flutamide was injected (10 mg/Kg b.w.) to adult male mice which were sacrificed 24h. and 72h. after. Controls injected with the vehicle (corn oil) were sacrificed at the same intervals. Cryosections were made of the epididymides and examined by the TUNEL method for quantification of apoptosis and also using immunocytochemistry to visualize the expression of the stress protein HSP70. The highest indexes of apoptosis were observed in the caput epididymis after 72 h. and were of 7.40 cells/1000 in contrast to controls (0.21 cells/1000). HSP70 appeared particularly increased in the caput and...
Tipo: Journal article
Palavras-chave: Antiandrogen; Apoptosis; Epididymis; Flutamide; Stress proteins.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200025

Reproductive Response in Offspring Male Rats Exposed to Prenatal Stress and to Early Postnatal Stimulation
Provedor de dados: 77
Autores: Chen Cárdenas,S. M; Mayer,N; Romanini,M. C; Rolando,A. N; Liaudat,A. C; Brun,N; Vivas,A; Gauna,H. F; Rodríguez,N.
Stress in pregnant rats alters the pattern of secretion of corticosterone (COR) and modifies transplacentally hypothalamic-pituitary-adrenal axis (HPA) fetus. Prenatal stress during the critical hypothalamic differentiation is related to decreased fertility of male offspring by an increase in the basal level of COR. This modification could induce long-term changes in the process of apoptosis in the testis. However, early postnatal handling increases maternal behavior and could reverse the effects caused by increased secretion of COR. The aim of this research was to investigate the effects of early postnatal stimulation of male rats prenatal stressed by chronic immobilization during the last two weeks of pregnancy, on the hypothalamic-pituitary-gonadal axis...
Tipo: Journal article
Palavras-chave: Early postnatal stimulation; Prenatal stress; HPA axis; HPG axis; Rats; Apoptosis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200065

Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice
Provedor de dados: 77
Autores: Asadi,Ebrahim; Shabani,Ronak; Ghafari,Soraya; Golalipour,Mohammad Jafar.
Several studies have shown that Morphine Sulfate affects on fertility, embryogenesis and consequent pregnancy loss and ultrastructural alterations of oocytes in animal model. This study was done to determine the effect of morphine sulfate on oocytes apoptosis and preventive role of daily supplementation of Vitamin E on oocytes apoptosis in morphine sulfate -treated mice. Twenty-four NMARI female mice were randomly allocated into four experimental groups. For 15 days, control group received saline (0.2 ml/day by subcutaneous injection), group I Vitamin E (60 mg/kg/day orally), group II Morphine Sulfate (10 mg/kg/day by subcutaneous injection) and group III Morphine Sulfate with Vitamin E (60 mg/kg/day orally). Then, animals were superovulated with PSMG (10...
Tipo: Journal article
Palavras-chave: Oocytes; Morphine sulfate; Vitamin E; Apoptosis; TUNEL assay; Mice.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200029

Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes
Provedor de dados: 77
Autores: Najafdari,Simin; Rezaei,Noorallah; Shafaroodi,Majid Malekzadeh; Ghafari,Soraya; Golalipour,Mohammad Jafar.
Previous studies have shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal models. This study was conducted to determine the effect of gestational diabetes on retinal ganglionic cell density, the thickness of the retinal layer and apoptotic ganglionic cell density in 28-day-old of rat offspring. In this experimental study, 10 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally, dams in control group received an equivalent volume normal saline. At postnatal day 28, six offspring of each gestational diabetes and controls were randomly selected, sacrificed and sections (6...
Tipo: Journal article
Palavras-chave: Gestational diabetes; Apoptosis; Retinal layer; Ganglionic cells; TUNEL Assay; Rat offspring.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400001

Excretion Product of Shigella dysenteriae (SdyEP) Induced Cell Death in Early Larval Stage of Zebrafish (Danio rerio): Acridine Orange and Ethidium Bromide (AO/EB) in vivo Staining
Provedor de dados: 77
Autores: Álvarez,M; Urbina,G; Perdomo,L.
In the field of studies of acute toxicity induced by bacterial agents, Shiga toxins have been relevant due to the severity of the extra-intestinal diseases they cause. Numerous studies have shown that Shiga toxin induced apoptosis in different cell types; however, this important process has been little studied in vivo experimental models. In this study, the effects of excretion products of Shigella dysenteriae, in which Shiga toxin is present, we investigated on early larval stages of Zebrafish, an animal model with many advantages over other in vivo experimental models traditionally used. Both the collection of eggs and larvae of Zebrafish, and the product from excretion from Shigella dysenteriae (SdyEP) were performed according to laboratory standards....
Tipo: Journal article
Palavras-chave: Zebrafish (Danio rerio); Shigella dysenteriae; Apoptosis; Acridine Orange; Ethidium Bromide.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400005

Perfil de Metilación de Genes Supresores de Tumores APAF1, ASSP1, p73 y FHIT en Pacientes con Leucemia Linfoblástica Aguda Infantil
Provedor de dados: 77
Autores: Melo A,Angélica; Artigas A,Carmen Gloria; Muñoz N,Sergio; Brebi M,Priscilla; Hoffstetter G,Rene; Roa S,Juan Carlos.
La leucemia linfoblástica aguda (LLA), es la neoplasia mas frecuente en la población infantil. Se manifiesta por una perdida de diferenciación de progenitores linfoides produciendo un aumento de células inmaduras. La hipermetilación en la región promotora de genes supresores de tumores (GST) puede producir un silenciamiento génico que le proporciona a la célula leucémica una ventaja proliferativa o la previene de la apoptosis. Se estudia el estado de hipermetilación de 4 GST involucrados en la apoptosis: APAF1, ASPP1, p73 y FHIT y su asociación con la sobrevida de pacientes menores de 15 años con diagnóstico de LLA. Se analizaron 38 muestras de médula ósea mediante modificación con bisulfito del ADN y reacción en cadena de la polimerasa especifica de...
Tipo: Journal article
Palavras-chave: Hipermetilación; Leucemia linfoblástica aguda Infantil; Genes supresores de tumores; Genes blanco de p53; Apoptosis; Sobrevida.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300033

Efecto de Dosis Única Intraperitoneal de Cipermetrina en la Corteza Cerebral Somatosensorial de Ratones CF- 1
Provedor de dados: 77
Autores: Jiménez Venegas,Leonella; Quilodrán Peredo,Javier; Miranda Olivares,Juan Pablo; Rodríguez Bustos,Héctor.
La cipermetrina es un pesticida ampliamente utilizado en agricultura y en salud pública. Sus efectos histopatológicos a nivel de sistema nervioso central han sido poco estudiados, aún cuando se han demostrado casos de intoxicación en humanos, con importante compromiso neurológico. El propósito de este trabajo es conocer los efectos de una dosis única intraperitoneal de cipermetrina sobre la morfología neuronal de corteza somatosensorial (láminas superficiales y profundas) de ratones CF-1. 35 ratones fueron separados en tres grupos: control (n=5), control vehículo (n=15) y experimental (n=15); los dos últimos grupos sacrificados los días 1, 8, 18, 26 y 35, en grupos de a tres. Los encéfalos fueron removidos, fijados, y procesados con técnica de rutina, para...
Tipo: Journal article
Palavras-chave: Corteza somatosensorial; Apoptosis; Cipermetrina; Neurotoxicidad.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000100003

Comparación de dos técnicas para la obtención de complejos cumulus ovocito porcinos*
Provedor de dados: 78
Autores: Lorenzo,MS; Tello,MF; Fischman,ML; Claver,J; Lombardo,DM.
La selección de ovocitos de calidad es clave para el éxito de la producción in vitro de embriones. La técnica de obtención de complejos cumulus ovocito (COC) parecería influir en la calidad de los ovocitos. En objetivo del presente trabajo fue comparar dos métodos de obtención de COC porcinos: slicing y aspiración folicular, determinando en cada caso, el porcentaje de maduración meiótica ovocitaria y la apoptosis temprana y tardía en el COC. Al utilizar aspiración folicular se obtuvieron porcentajes significativamente mayores en la maduración nuclear ovocitaria, sin embargo, esta técnica quedó restringida para folículos antrales grandes. Asimismo, se recuperó menor cantidad de ovocitos por ovario que por el método de slicing. Por otra parte, el desarrollo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COC porcinos; Apoptosis; Aspiración folicular; Slicing.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982015000100003

Disrupción de las uniones mediadas por caderinas. Su rol en la apoptosis de las células granulosas del ovario porcino
Provedor de dados: 78
Autores: Lombardo,DM; Medina,JM; Revilla,M; Carou,CM; Fiorito,CD.
La fisiología normal de los tejidos reproductivos dependería de un apropiado contacto célula-célula mediado por caderinas, moléculas Ca++ dependientes. El proceso de la atresia dado por apoptosis de las células de granulosa (CG), podría estar relacionado con la pérdida de los contactos celulares. El objetivo del trabajo fue establecer la relación entre la disrupción de estas uniones y la apoptosis de las CG. Considerando la importancia de las caderinas (E-CAM y N-CAM) en el desarrollo y en la atresia folicular, que la mayoría de los folículos empiezan a degenerar en el estadio antral temprano y considerando la atresia como un proceso estadio-dependiente se planteó identificar y localizar la expresión de estas moléculas en el ovario de cerdo adulto,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Células granulosas; Caderina E; Apoptosis; Ovario; Porcino.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982010000100012

Interferencia del Herpesvirus equino 1 (EHV-1) en la apoptosis inducida
Provedor de dados: 78
Autores: Scrochi,M.R.; Zanuzzi,C.N.; Muglia,C.I.; Fuentealba,N.A.; Nishida,F; Gimeno,E; Barbeito,C.G.; Portiansky,E.L.; Galosi,C.M.
La apoptosis es un tipo de muerte celular programada que puede ser desencadenada por múltiples factores, tanto internos como externos; dentro de estos últimos se encuentran las infecciones virales. Algunos alphaherpesvirus han desarrollado diversas estrategias para retardar o inhibir la muerte celular obteniendo, de esta manera, su propio beneficio al poder permanecer durante más tiempo en la célula. Hasta el momento no se ha identificado ningún mecanismo relacionado con la modulación de la muerte celular durante la infección con Herpesvirus equino tipo 1 (EHV-1). El objetivo del presente trabajo fue describir el efecto producido por la infección con EHV-1 sobre cultivos celulares inducidos a la muerte por apoptosis. La evaluación de la apoptosis se...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anexina V; Apoptosis; Citoqueratina 18; Fragmentación de ADN; Herpesvirus equino 1.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200001

Apoptosis inducida por un análogo de GnRH en una línea celular de granulosa de ovario bovino (BGC-1)
Provedor de dados: 78
Autores: Cruzans,P.R.; Carou,M.C.; Lorenzo,M.S.; Lombardo,D.M.
Se han identificado receptores de la hormona liberadora de gonadotrofina (GnRHR) en células de granulosa ovárica (CG) y en células luteales (CL). Asimismo, se ha observado que la activación de los GnRHR en CG regula la función gonadal. Estudios realizados utilizando agonistas de GnRH (GnRHa) en ovarios de ratas, in vivo e in vitro, demostraron un incremento de la atresia folicular. Nuestro objetivo fue estudiar la regulación de la apoptosis en una línea establecida de células de granulosa de ovario bovino (BGC-1) utilizando el acetato de leuprolide (LA) como GnRHa y el ANTIDE como antagonista competitivo. Nuestros resultados demuestran un aumento significativo de la proporción de células apoptóticas inducidas durante 24 y 48 h con 100 nM LA, confirmado...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; BGC-1; Acetato de leuprolide; GnRH.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982012000100011

In vitro apprach to the study of chronic exposure to low does of X-rays
Provedor de dados: 46
Autores: Ponzinibbio,M.V; Peral-García,P; Seoane,A.
The present research was undertaken in order to study the effect of repeated low doses of radiation on two different cell lines from the same species. An in vitro model test was developed to avoid the influence of the confounding factors affecting epidemiological studies and to simulate a chronic exposure (50 mSv of x-rays during ten consecutive days). Comet assay and early apoptosis were analyzed immediately after exposure and after chronic irradiation. Sequential irradiation induced an increase of cells showing DNA damage. Index Damage was higher than that of the controls in both cell lines immediately after exposure and after chronic irradiation. These differences between exposed and control cells were statistically significant only for the transformed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Comet assay; In vitro; Ionizing radiation; Low dose.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000200004

Cytotoxic and apoptotic effects of scorpion Leiurus quinquestriatus venom on 293T and C2C12 eukaryotic cell lines
Provedor de dados: 80
Autores: Omran,M. A. A.
Scorpion venom toxicity is of major concern due to its influence on human activities and public health. The cytotoxicity and apoptosis induced by scorpion L. quinquestriatus venom on two established eukaryotic cell lines (293T and C2C12) were analyzed. Both cultured cell lines were incubated with varying doses (10, 20, and 50 µg/ml) of scorpion venom in serum free medium (SFM) for 0.5, 1, 2, 4, and 8 hours at 37°C. The percentage of total lactate dehydrogenase (LDH) released in the culture during venom incubation was used as an index of cell damage. Control culture was treated with an equal amount of SFM. Cell injury was recognized morphologically and apoptosis was researched by a Fluorescing Apoptosis Detection System using the principle of TUNEL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Leiurus quinquestriatus; Cytotoxicity; Apoptosis; LDH.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200008

Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines
Provedor de dados: 80
Autores: Silva,Cássio Prinholato da; Costa,Tássia R.; Paiva,Raquel M. Alves; Cintra,Adélia C. O.; Menaldo,Danilo L.; Antunes,Lusânia M. Greggi; Sampaio,Suely V.
Abstract  Background  Phospholipases A 2 (PLA 2 s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA 2isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines.  Methods  The cytotoxic effects of BthTX-I were evaluated on the tumor cell lines HL-60 (promyelocytic leukemia), HepG2 (human hepatocellular carcinoma), PC-12 (murine pheochromocytoma) and B16F10 (murine melanoma) using the MTT method. Flow cytometry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; BthTX-I; Antitumor potential; Apoptosis; Cell cycle alterations.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100348

Integrins, cancer and snake toxins (mini-review)
Provedor de dados: 80
Autores: Rádis-Baptista,G.
Integrins encompass a family of transmembrane heterodimeric proteins of adhesion that maintain cells attached to other cells and to the extracellular matrix (ECM). Integrins work as bi-directional mechanotransducers, conveying mechanical signal from outside to inside the cell through a cascade of phosphorylation signals. On the other hand, the signal from inside to outside controls the strength and affinity of integrin adhesion. As proteins of focal contact, integrins are involved in diverse cell functions, such as cell activation, migration, growth, and survival. In the development of neoplastic disease and metastatic tumor, integrins can influence cancer invasiveness and progression, as well as mediate the formation of new blood vessels (angiogenesis)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Integrin; Angiogenesis; Cancer; Apoptosis; Snake toxin; Snake venom C-type lectin; Metalloprotease; Disintegrin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300002

Cytotoxic activity of Androctonus australis hector venom and its toxic fractions on human lung cancer cell line
Provedor de dados: 80
Autores: Béchohra,Louisa; Laraba-Djebari,Fatima; Hammoudi-Triki,Djelila.
Abstract  Background: Several studies have showed that animal venoms are a source of bioactive compounds that may inhibit the growth of cancer cells, which makes them useful agents for therapeutic applications. Recently, it was established that venom toxins from scorpions induced cytotoxic, antiproliferative and apoptogenic effects on cancer cells. Therefore, the present study aims to investigate the cytotoxic activity of Androctonus australis hector (Aah) scorpion venom and its toxic fractions (FtoxG-50 and F3) on NCI-H358 human lung cancer cells.  Methods: The cytotoxic and antiproliferative activities were estimated using MTT assay, lactate dehydrogenase release and clonogenic assays. Apoptosis was evaluated by Hoechst 33258 staining, DNA fragmentation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aah venom; Cytotoxicity; F3 fraction; Apoptosis; Lung cancer cells; Oxidative stress.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100322

Potentiation of a novel palladium (II) complex lethality with bee venom on the human T-cell acute lymphoblastic leukemia cell line (MOLT-4)
Provedor de dados: 80
Autores: Safaeinejad,Zahra; Mohammad Nabiuni,; Nazari,Zahra.
Background : Although honeybee venom (BV) has been reported to induce apoptosis in different types of cancerous cells, its synergistic effects with customary anti-cancer drugs remain largely unknown. In the present study, we evaluated the cytotoxic effect of BV alone (as a natural product) and the synergistic cytological effects of this component in combination with [Pd (bpy) (Pi-Pydtc)]NO3 - a novel palladium complex on human T-cell lymphoblastic leukemia cells. To investigate the cytotoxic effect of the BV alone and in combination with palladium complex on MOLT-4 cells MTT assay was performed. In order to determine the apoptotic effects of BV separately and in combination with Pd (II) complex on these cells and its ability to induce apoptosis,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Bee venoms; Cytotoxicity; MOLT-4 cell line; Pd (II) complex.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100315

Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus
Provedor de dados: 80
Autores: Contreras-Ortiz,José María Eloy; Vázquez-Chagoyán,Juan Carlos; Martínez-Castañeda,José Simón; Estrada-Franco,José Guillermo; Aparicio-Burgos,José Esteban; Acosta-Dibarrat,Jorge; Barbabosa-Pliego,Alberto.
Background : The venom of Centruroides limpidus limpidus (Cll) is a mixture of pharmacologically active principles. The most important of these are toxic proteins that interact both selectively and specifically with different cellular targets such as ion channels. Recently, anticancer properties of the venom from other scorpion species have been described. Studies in vitro have shown that scorpion venom induces cell death, inhibits proliferation and triggers the apoptotic pathway in different cancer cell lines. Herein, after treating human cervical adenocarcinoma (HeLa) cells with Cll crude venom, their cytotoxic activity and apoptosis induction were assessed. Results : Cll crude venom induced cell death in normal macrophages in a dose-dependent manner....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venoms; Cytotoxic tests; Apoptosis; HeLa cells; Anticancer agents; Centruroides toxins.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100311

BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation
Provedor de dados: 80
Autores: Bezerra,Patrícia Heloise Alves; Ferreira,Isadora Marques; Franceschi,Beatriz Tinoco; Bianchini,Francine; Ambrósio,Luciana; Cintra,Adélia Cristina O.; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Torqueti,Maria Regina.
ABSTRACT  Background: Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer cell lines.  Methods:  BthTX-I cytotoxicity was determined via MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay. Cell death was measured by a hypotonic fluorescent solution method, annexin-V-FITC/propidium iodide staining and by apoptotic/autophagic protein expression. Cancer stem cells (CSCs) were quantified by flow cytometry using anti-CD24-FITC and anti-CD44-APC antibodies and propidium iodide.  Results:  BthTX-I at 102 µg/mL induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Bothropstoxin; Breast cancer; Cancer stem cells.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100315

Hepatotoxicity and oxidative stress induced byNaja haje crude venom
Provedor de dados: 80
Autores: Al-Quraishy,Saleh; Dkhil,Mahamed A; Moneim,Ahmed Esmat Abdel.
Background Snake venoms are synthesized and stored in venom glands. Most venoms are complex mixtures of several proteins, peptides, enzymes, toxins and non-protein components. In the present study, we investigated the oxidative stress and apoptosis in rat liver cells provoked by Naja haje crude injection (LD50) after four hours.Methods Wistar rats were randomly divided into two groups, the control group was intraperitoneally injected with saline solution while LD50-dose envenomed group was intraperitoneally injected with venom at a dose of 0.025 μg/kg of body weight. Animals were killed four hours after the injection. Lipid peroxidation, nitric oxide and glutathione levels were measured as oxidative markers in serum and liver homogenate. In addition, liver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naja haje venom; Hepatotoxicity; Oxidative stress; Apoptosis; Rats.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200314

Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells
Provedor de dados: 80
Autores: Shahbazi,Benyamin; Najafabadi,Zahra Salehi; Goudarzi,Hamidreza; Sajadi,Mahnaz; Tahoori,Fatemeh; Bagheri,Masoumeh.
ABSTRACT  Background:  Several studies have pointed out that certain snake venoms contain compounds presenting cytotoxic activities that selectively interfere with cancer cell metabolism. In this study, Pseudocerastes persicus venom and its fractions were investigated for their anticancer potential on lung cancer cells.  Methods: Lung cancer cells (A549) and normal fibroblast cells (Hu02) were treated with the P. persicus venom and its HPLC fractions and the cell cytotoxic effects were analyzed using MTT and lactate dehydrogenase release assays. Apoptosis was determined in venom-treated cell cultures using caspase-3 and caspase-9 assay kits.  Results:  The treatment of cells with HPLC fraction 21 (25-35 kDa) of P. persicus venom resulted in high LDH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudocerastes persicus venom; Persian horned viper; HPLC fractions; Cytotoxicity; Apoptosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100319

Histopathological characterization of experimentally induced cutaneous loxoscelism in rabbits inoculated with Loxosceles similis venom
Provedor de dados: 80
Autores: Pereira,NB; Kalapothakis,E; Vasconcelos,AC; Chatzaki,M; Campos,LP; Vieira,FO; Verçosa,BLA; Silva,SS; Ferreira,WM; Moro,L.
Envenomation by Loxosceles bites is characterized by dermonecrotic and/or systemic features that lead to several clinical signs and symptoms called loxoscelism. Dermonecrotic lesions are preceded by thrombosis of the dermal plexus. Recent studies show that atheromatous plaque is prone to thrombosis due to endothelial cell apoptosis. To the best of our knowledge, there are no reports of microscopic dermal lesion and endothelial cell apoptosis induced by Loxosceles similis venom in the literature. Thus, the aim of the present study is to describe histological lesions induced by L. similis venom in rabbit skin and to elucidate whether apoptosis of endothelial cells is involved in the pathogenesis of loxoscelism. Forty male rabbits were split into two groups:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loxosceles similis; Loxoscelism; Dermonecrosis; Cutaneous loxoscelism; Apoptosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300005

Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
Provedor de dados: 80
Autores: Bustillo,S; Lucero,H; Leiva,LC; Acosta,O; Kier Joffé,EB; Gorodner,JO.
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops alternatus; Bothrops diporus; Cytotoxicity; C2C12; Apoptosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100004

Snake venom L-amino acid oxidases: an overview on their antitumor effects
Provedor de dados: 80
Autores: Costa,Tássia R; Burin,Sandra M; Menaldo,Danilo L; Castro,Fabíola A de; Sampaio,Suely V.
The L-amino acid oxidases (LAAOs) constitute a major component of snake venoms and have been widely studied due to their widespread presence and various effects, such as apoptosis induction, cytotoxicity, induction and/or inhibition of platelet aggregation, hemorrhage, hemolysis, edema, as well as antimicrobial, antiparasitic and anti-HIV activities. The isolated and characterized snake venom LAAOs have become important research targets due to their potential biotechnological applications in pursuit for new drugs of interest in the scientific and medical fields. The current study discusses the antitumor effects of snake venom LAAOs described in the literature to date, highlighting the mechanisms of apoptosis induction proposed for this class of proteins.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; L-amino acid oxidases; Antitumor effects; Apoptosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200206

Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment
Provedor de dados: 80
Autores: Tamieti,B. P.; Damatta,R. A.; Cogo,J. C.; Da Silva,N. S.; Mittmann,J.; Pacheco-Soares,C.
Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell culture; Actin filaments; Nucleus; Endoplasmic reticulum; Crotalus; Rattlesnake venom; Apoptosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100004

Cytotoxic and pro-apoptotic action of MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, towards leukemic cells
Provedor de dados: 80
Autores: Benati,Rogério Bodini; Costa,Tássia Rafaela; Cacemiro,Maira da Costa; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Burin,Sandra Mara.
Abstract  Background: Chronic myeloid leukemia (CML) is a BCR-ABL1+ myeloproliferative neoplasm marked by increased myeloproliferation and presence of leukemic cells resistant to apoptosis. The current first-line therapy for CML is administration of the tyrosine kinase inhibitors imatinib mesylate, dasatinib or nilotinib. Although effective to treat CML, some patients have become resistant to this therapy, leading to disease progression and death. Thus, the discovery of new compounds to improve CML therapy is still challenging. Here we addressed whether MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, affects the viability of imatinib mesylate-resistant Bcr-Abl+ cell lines.  Methods: We examined the cytotoxic and pro-apoptotic effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic myeloid leukemia; Bcr-Abl; Phospholipase A2; MjTX-I; Bothrops moojeni; Apoptosis; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100330

Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)
Provedor de dados: 80
Autores: Meira,DA; Almeida,RAMB; Barbosa,AN; de Souza,LR; Olivo,TET; Henriques,RMS; Golim,MA; Araújo Jr,JP; Nagoshi,LR; Orikaza,CM; Calvi,SA.
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RT-PCR; ELISA; Cytokines; HIV; AIDS; HAART; Apoptosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011

Biochemical and morphological analysis of cell death induced by Egyptian cobra (Naja haje) venom on cultured cells
Provedor de dados: 80
Autores: Omran,M. A. A.; Fabb,S. A.; Dickson,G.
We investigated the in vitro process of cell death caused by Egyptian cobra venom on primary human embryonic kidney (293T) and mouse myoblast (C2C12) cell lines. The aim of these studies was to provide further information about triggering cell death, and suggest methods for eliminating unwanted cells, such as tumour cells. Both cell lines were treated with 10, 20, and 50 m g/ml of Egyptian cobra (Naja haje) venom in serum free media (SFM) and incubated for 8 hours. Total activities of the lactate dehydrogenase (LDH) and creatine kinase (CK) released in the culture during venom incubation were used as an indicator of the venom in vitro cytotoxicity. Cell injury was morphologically recognized and apoptosis determined by a Fluorescing Apoptosis Detection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cobra venom; Cytotoxicity; Apoptosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300004

Comparison of Enterotoxicity between Autumn Crocus (Colchicum autumnale L.) and Colchicine in the Guinea Pig and Mouse : Enterotoxicity in the Guinea Pig Differs from That in the Mouse
Provedor de dados: 19
Autores: YAMADA, Manabu; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; MATSUI, Takane; 古林, 与志安; 古岡, 秀文.
Autumn crocus poisoning of cattle is characterized by severe diarrhea caused by alkaloid colchicine. Previously, we examined pathologically this poisoning in cattle and reported that enterotoxic lesions were closely associated with apoptosis. To examine enterotoxicity of autumn crocus more precisely, a reproductive study was performed using guinea pigs and mice, and pathological findings associated with autumn crocus poisoning were compared with those of colchicine. Each group of guinea pigs given the bulb of autumn crocus or colchicine exhibited severe diarrhea. Histopathological findings in intoxicated guinea pigs were entirely consistent with those in the autumn crocus-poisoned cattle. In contrast, each group of mice administered with the bulb or...
Palavras-chave: Apoptosis; Autumn crocus (Colchicum autumnale L.); Colchicine; Guinea pig; Mouse.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4170

Effect of intraluteal injection of endothelin type A receptor antagonist on PGF2alpha-induced luteolysis in the cow.
Provedor de dados: 19
Autores: Watanabe, Sho; Shirasuna, Koumei; Matsui, Motozumi; Yamamoto, Dai; Berisha, Bajram; Schams, Dieter; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫.
Endothelin-1 (ET-1) is a luteolytic mediator in the bovine corpus luteum (CL), and its action appears to be via endothelin type A receptor (ETR-A). Thus, the aim of the present study was to determine the effect of ETR-A antagonist on PGF(2)alpha-induced luteolysis in the cow. Cows on days 1012 of the estrous cycle were subjected to five intraluteal injections of the ETR-A antagonist LU 135252 in saline or only saline at -0.5, 2, 4, 6, and 8 h after PGF(2)alpha administration (=0 h). Serial luteal biopsies were conducted to determine the expression of mRNA in the luteal tissue. There were no significant differences in the decrease in plasma progesterone (P) concentrations and the mRNA expressions of steroidogenic acute regulatory protein and 3...
Palavras-chave: Apoptosis; Blood flow; Corpus luteum; Cow; Endothelin-1.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/922

The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells
Provedor de dados: 89
Autores: Ibtisham,F; Zhao,Y; Nawab,A; Liguang,H; Wu,J; Xiao,M; Zhao,Z; An,L.
ABSTRACT The effects of oxidative stress induced by high temperature on the cell viability, proliferation, apoptosis and oxidative status of chicken embryonic fibroblasts (CEF) were analyzed. The viability, proliferation, apoptotic and anti-oxidative status were measured after incubating CEF at the temperatures of 37ºC (control) and 40-44ºC (experimental groups) for 6,12 and 24 hours. The results showed that at high temperature (42-43ºC), the viability of CEF cells decreased after 6, 12 and 24 h of incubation, but the difference was significant only at 43ºC. Cell proliferation was significantly reduced at 44oC/6h. The apoptotic rate of CEF cells was increased following heat treatments in a time-dependent manner. ROS formation increased with increasing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Chicken embryonic fibroblast; Cell proliferation; Heat stress; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300463

Epigallocatechin Gallate (EGCG) Inhibited the Alv-J-Induced Apoptosis in Df-1 Cells by Inactivation of Nuclear Factor κb Pathway
Provedor de dados: 89
Autores: Zhang,Y; Yang,H; Wu,X; Deng,M; Li,Z; Xu,Z.
ABSTRACT Avian leukosis virus subgroup J (ALV-J), a member of the retroviridae family, can infect both broilers and layers and induce a spectrum of different neoplasms, resulting in serious economic losses in poultry production. Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, has demonstrated remarkable anti-inflammatory and cancer chemopreventive effects in many animal tumor bioassays, cell culture systems and epidemiological studies. To assess the antiviral effects of EGCG on ALV-J-induced cell apoptosis in vitro, DF-1 cells were treated with different EGCG concentrations (0, 5, 10, 20 and 40 µg/mL), and their antiviral effects were examined at different time points (0, 24, 48, 72 and 96 h) using a variety of assays. EGCG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ALV-J; EGCG; NF-kB; DF-1 cell; Apoptosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100323

REPARACIÓN DEL ADN: UNA POSIBLE RELACIÓN ENTRE LA DEFICIENCIA DE FOLATO Y LA MUERTE NEURONAL
Provedor de dados: 37
Autores: RAMÍREZ,NELSON J; ARBOLEDA,GONZALO; ARBOLEDA,HUMBERTO.
El presente artículo explora el papel que desempeña el folato como conocido metabolito del ciclo de un carbono (OCM, del inglés onecarbon metabolism) en la alteración de la integridad de las células nerviosas. Aquí se discute evidencia reciente de la literatura que muestra la reparación del ADN como un proceso relacionado con la apoptosis neuronal inducida por ausencia de folato.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Metabolismo; ADN glicosilasas; Deficiencia de ácido fólico; Reparación del ADN.
Ano: 2007
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2007000200011

ACTIVIDAD ANTIPROLIFERATIVA DE ACEITES ESENCIALES DE PLANTAS CULTIVADAS EN COLOMBIA
Provedor de dados: 37
Autores: VELANDIA,Sindi Alejandra; QUINTERO,Elizabeth; STASHENKO,Elena E; OCAZIONEZ,Raquel E.
RESUMEN Colombia posee gran diversidad de plantas medicinales, pero pocas han sido objeto de investigación. En este trabajo se evaluó la actividad antiproliferativa de aceites esenciales obtenidos por hidrodestilación asistida por microondas. Se analizaron 15 muestras de 11 especies en ensayos del MTT en células cancerosas MCF-7, HeLa y HepG-2 y se incluyeron células normales humanas (HEK293) y de animales (Vero y B16F10) para evaluar selectividad. La composición química de muestras activas se determinó por cromatografía de gases acoplada a espectrometría de masas (GC-MS). Aceites esenciales de cuatro especies mostraron actividad antiproliferativa (CI50: 50 μg/mL) en células HeLa y/o MCF-7, en el siguiente rango (índice de selectividad en paréntesis):...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aceite esencial; Apoptosis; Cáncer; Fitoterapia; MTT.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2018000200189

Establecimiento de la línea PL104 Modelo de estudio del balance muerte/sobrevida de células tumorales
Provedor de dados: 38
Autores: Cavaliere,Victoria; Glickman,Ariel; Solari,Liliana; Gammella,Daniel; Hajos,Silvia; Álvarez,Élida.
La apoptosis es un proceso fisiológico crítico para el crecimiento celular, regulado por factores pro y anti-apoptóticos. Defectos en su control llevan a una ventaja selectiva en la sobrevida celular favoreciendo el desarrollo de patologías neoplásicas. NFkB puede promover la proliferación celular por lo que el uso de inhibidores selectivos lleva a un aumento de la apoptosis. El objetivo del presente trabajo fue caracterizar la línea celular PL104, obtenida de células de punción de médula ósea de una paciente con diagnóstico de leucemia mieloide aguda (LMA) atípica y evaluar la susceptibilidad de la misma frente a los quimioterápicos Doxorubicina (DOX), Vincristina (VCR), Gemcitabina e inhibidores de NFkB, CAPE y MG-132. El fenotipo evaluado por citometría...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leucemia mieloide aguda; Apoptosis; Factor de trascripción nuclear kB; Doxorubicina; Vincristina; Gemcitabina; Ácido cafein fenil etil éster; MG-132.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572006000100004

Active caspase-3 expression levels as bioindicator of individual radiosensitivity
Provedor de dados: 42
Autores: SANTOS,NEYLIANE F.G. DOS; SILVA,RAFAEL F.; PINTO,MARCELA M.P.L.; SILVA,EDVANE B. DA; TASAT,DEBORAH R.; AMARAL,ADEMIR.
ABSTRACT Several molecules and events involved in cell response to radiation-induced damage have been investigated towards a personalized radiotherapy. Considering the importance of active caspase-3 in the proteolytic cascade that ensures radiation-induced apoptosis execution, this research was designed to evaluate the expression levels of this protein as a bioindicator of individual radiosensitivity. Peripheral blood samples of 10 healthy individuals were gamma-irradiated (cobalt-60 source) with 1, 2 and 4 Gy (control: non-irradiated samples), and active caspase-3 expression levels were measured in lymphocytes, by flow cytometry, ex vivo and after different times of in vitro incubation (24, 48 and 72 hours). Short-term incubation of 24 h was the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Active caspase-3; Apoptosis; Flow cytometry; Ionizing radiation; Lymphocytes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200649

Inhibition of caspase-8 activity reduces IFN-gamma expression by T cells from Leishmania major infection
Provedor de dados: 42
Autores: Pereira,Wânia F.; Guillermo,Landi V.C.; Ribeiro-Gomes,Flávia L.; Lopes,Marcela F.
Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodes of C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression following Tcellactivation. After two weeks of infection, apoptotic T cells were not detected in draining lymph nodes but activation with anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-FasLigand, caspase-8 or caspase- 9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8 activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8 T cells expressed IFN-gamma upon activation. Treatment with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Caspase-8; CD8 T cells; IFN-gamma; Leishmaniasis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100008

Apoptotic cell and phagocyte interplay: recognition and consequences in different cell systems
Provedor de dados: 42
Autores: Moreira,Maria Elisabete C.; Barcinski,Marcello A.
Cell death by apoptosis is characterized by specific biochemical changes, including the exposure of multiple ligands, expected to tag the dying cell for prompt recognition by phagocytes. In non-pathological conditions, an efficient clearance is assured by the redundant interaction between apoptotic cell ligands and multiple receptor molecules present on the engulfing cell surface. This review concentrates on the molecular interactions operating in mammalian and non-mammalian systems for apoptotic cell recognition, as well as on the consequences of their signaling. Furthermore, some cellular models where the exposure of the phosphatidylserine (PS) phospholipid, a classical hallmark of the apoptotic phenotype, is not followed by cell death will be discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell death; Apoptosis; Apoptotic cell recognition; Apoptotic mimicry; Phagocyte; Intracellular signaling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000100009

Understanding the mechanisms of glutamine action in critically ill patients
Provedor de dados: 42
Autores: Oliveira,Gisele P.; Dias,Cristina M.; Pelosi,Paolo; Rocco,Patricia R.M.
Glutamine (Gln) is an important energy source and has been used as a supplementary energy substrate. Furthermore, Gln is an essential component for numerous metabolic functions, including acid-base homeostasis, gluconeogenesis, nitrogen transport and synthesis of proteins and nucleic acids. Therefore, glutamine plays a significant role in cell homeostasis and organ metabolism. This article aims to review the mechanisms of glutamine action during severe illnesses. In critically ill patients, the increase in mortality was associated with a decreased plasma Gln concentration. During catabolic stress, Gln consumption rate exceeds the supply, and both plasma and skeletal muscle pools of free Gln are severely reduced. The dose and route of Gln administration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat shock protein; Apoptosis; Cytokines; Glutamine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200018

Susceptible hosts: a resort for parasites right in the eye of the immune response
Provedor de dados: 42
Autores: DOSREIS,GEORGE A.
Trypanosomatid protozoan parasites express an aggressive strategy of parasitism by infecting host macrophages and inducing extensive T-lymphocyte activation. One goal of such strategy is to drive the immune response of genetically susceptible hosts to a state of unresponsiveness regarding parasite killing. Unresponsiveness is achieved through different mechanisms, depending on the parasite species. In this brief review, recent findings on the molecular and cellular bases of the parasites' exploitation of host immune responses are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Leishmania chagasi; T lymphocytes; CTLA-4; Apoptosis; Transforming growth factor.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000100011

Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells
Provedor de dados: 42
Autores: GARCIA,CHARLENE S.C.; MENTI,CAROLINE; LAMBERT,ANA PAULA F.; BARCELLOS,THIAGO; MOURA,SIDNEI; CALLONI,CAROLINE; BRANCO,CÁTIA S.; SALVADOR,MIRIAN; ROESCH-ELY,MARIANA; HENRIQUES,JOÃO A.P.
ABSTRACT Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Apoptosis; Cancer; Chemical compounds; Salvia officinalis (L.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100281

Caspase-3 activation and increased procollagen type I in irradiated hearts
Provedor de dados: 42
Autores: Ferreira-Machado,Samara C.; Salata,Camila; Rocha,Nazareth N.; Corrêa,Alexandre F.S.; Côrte-Real,Suzana; Peregrino,Antônio A.F.; Campos,Vera M.A. de; Andrade,Cherley B.V.; Bernardo-Filho,Mario; Cabral-Neto,Januário B.; Dealmeida,Carlos E.
The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Caspase-3; Fibrosis; Heart; Ionizing radiation; TGF-β1.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100215

Antifungal and antitumor models of bioactive protective peptides
Provedor de dados: 42
Autores: Rodrigues,Elaine G.; Dobroff,Andrey S.; Taborda,Carlos P.; Travassos,Luiz R.
Peptides are remarkably reactive molecules produced by a great variety of species and able to display a number of functions in uni-and multicellular organisms as mediators, agonists and regulating substances. Some of them exert cytotoxic effects on cells other than those that produced them, and may have a role in controlling subpopulations and protecting certain species or cell types. Presently, we focus on antifungal and antitumor peptides and discuss a few models in which specific sequences and structures exerted direct inhibitory effects or stimulated a protective immune response. The killer peptide, deduced from an antiidiotypic antibody, with several antimicrobial activities and other Ig-derived peptides with cytotoxic activities including antitumor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioactive peptides; Paracoccidioides brasiliensis; Tumor cells; Killer peptide; Melanoma; Apoptosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300015

The T-cell anergy induced by Leishmania amazonensis antigens is related with defective antigen presentation and apoptosis
Provedor de dados: 42
Autores: Pinheiro,Roberta O.; Pinto,Eduardo F.; Benedito,Alessandra B.; Lopes,Ulisses G.; Rossi-Bergmann,Bartira.
Leishmania amazonensis is the main agent of diffuse cutaneous leishmaniasis, a disease associated with anergic immune responses. In this study we show that the crude antigen of Leishmania amazonensis (LaAg) but not L. braziliensis promastigotes (LbAg) contains substances that suppress mitogenic and spontaneous proliferative responses of T cells. The suppressive substances in LaAg are thermoresistant (100ºC/1h) and partially dependent on protease activity. T cell anergy was not due to a decreased production of growth factors as it was not reverted by addition of exogenous IL-2, IL-4, IFN-gamma or IL-12. LaAg did not inhibit anti-CD3-induced T cell activation, suggesting that anergy was due to a defect in antigen presentation. It was also not due to cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Anergy; Apoptosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000300006

Diosmin induces caspase-dependent apoptosis in human glioblastoma cells
Provedor de dados: 42
Autores: SOARES,JULIANA M.; FARIA,BRUNA M. DE; ASCARI,LUCAS M.; ALVES-LEON,SONIZA V.; SOUZA,JORGE M. DE; SOARES,ANTONIO G.; CORDEIRO,YRAIMA; ROMÃO,LUCIANA F.
Abstract: Diosmin is a flavone glycoside clinically used as the main component of Daflon for the treatment of venous diseases. Several studies demonstrated that this natural compound can induce apoptosis in different tumors. However, isolated diosmin has not been studied regarding its effects on glioblastoma so far. Since glioblastoma is a highly lethal and fast-growing brain tumor, new therapeutic strategies are urgently needed. Herein, we evaluated the role of this flavonoid against glioblastoma cells using in vitro assays. Diosmin significantly reduced the viability of GBM95, GBM02, and U87MG glioblastoma cells, but not of healthy human astrocytes, as verified by MTT assay. Vimentin immunostaining showed that diosmin induced morphological changes in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Diosmin; Flavonoid; Glioblastoma.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700701

Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
Provedor de dados: 42
Autores: Esteves,Mabel B.; Marques-Santos,Luis F.; Affonso-Mitidieri,Ottília R.; Rumjanek,Vivian M.
Lymphocytes activated by mitogenic lectins display changes in transmembrane potential, an elevation in the cytoplasmic Ca2+ concentrations, proliferation and/or activation induced cell death. Low concentrations of ouabain (an inhibitor of Na+,K+-ATPase) suppress mitogen-induced proliferation and increases cell death. To understand the mechanisms involved, a number of parameters were analyzed using fluorescent probes and flow cytometry. The addition of 100nM ouabain to cultures of peripheral blood lymphocytes activated with 5µg/ml phytohemagglutinin (PHA) did not modify the increased expression of the Fas receptor or its ligand FasL induced by the mitogen. However, treatment with ouabain potentiated apoptosis induced by an anti-Fas agonist antibody. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocytes; Ouabain; PHA; Apoptosis; Membrane depolarization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000200008

India ink induces apoptosis in the yellow clam Mesodesma mactroides (Deshayes, 1854). Optical and ultrastructural study
Provedor de dados: 42
Autores: CARVALHO,YURI B.M.; JETHRO,JUAN; POERSCH,LUIS H.; ROMANO,LUIS A.
ABSTRACT This paper reports on the acute inflammatory and cellular process in the yellow clam, Mesodesma mactroides, induced by injection of India ink into the muscular foot. Histological observations with optical and electronic microscopy were made at 24 and 48 h after injection. The induced cellular inflammatory response consisted of a general hemocyte infiltration without necrosis and apoptotic activity. Migration of ink-laden phagocytes across the intestinal epithelium was recorded. It appeared that the yellow clam "excreted" ink particles through the gill and kidney. The positive staining for apoptosis was observed in the digestive gland. Electronic microscopy revealed ultrastructural changes of endoplasmic reticulum stress and apoptotic bodies in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: India ink; Inflammation process; Endoplasmic reticulum stress; Apoptosis; Mesodesmama mactroides.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000501981

Modulatory effects of rutin on biochemical and hematological parameters in hypercholesterolemic Golden Syrian hamsters
Provedor de dados: 42
Autores: Kanashiro,Alexandre; Andrade,Daiani C.O.; Kabeya,Luciana M.; Turato,Walter M.; Faccioli,Lucia H.; Uyemura,Sérgio A.; Lucisano-Valim,Yara M.
Flavonoids have been reported to exhibit several pharmacological properties, mainly in cardiovascular and inflammatory diseases. In the present study, we observed that rutin, a known glycosylated flavonoid isolated from Dimorphandra mollis, had a lowering effect on plasma triglyceride levels of diet-induced hypercholesterolemic Golden Syrian hamsters, but did not change total cholesterol and high-density lipoprotein cholesterol levels. Moreover, high-fat or rutin supplemented diets showed no immunotoxic effects, since no significant changes were observed on total white blood cells, granulocytes and mononuclear cells, as well as on the neutrophil apoptosis degree, when compared to untreated animals. Therefore, rutin seems to be a selective and non-toxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rutin; Golden Syrian hamster; Hypercholesterolemia; Atherosclerosis; Apoptosis; Dimorphandra mollis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000100009

Calcium and cell death signaling in neurodegeneration and aging
Provedor de dados: 42
Autores: Smaili,Soraya; Hirata,Hanako; Ureshino,Rodrigo; Monteforte,Priscila T.; Morales,Ana P.; Muler,Mari L.; Terashima,Juliana; Oseki,Karen; Rosenstock,Tatiana R.; Lopes,Guiomar S.; Bincoletto,Claudia.
Transient increase in cytosolic (Cac2+) and mitochondrial Ca2+ (Ca m2+) are essential elements in the control of many physiological processes. However, sustained increases in Ca c2+ and Ca m2+ may contribute to oxidative stress and cell death. Several events are related to the increase in Ca m2+, including regulation and activation of a number of Ca2+ dependent enzymes, such as phospholipases, proteases and nucleases. Mitochondria and endoplasmic reticulum (ER) play pivotal roles in the maintenance of intracellular Ca2+ homeostasis and regulation of cell death. Several lines of evidence have shown that, in the presence of some apoptotic stimuli, the activation of mitochondrial processes maylead to the release of cytochrome c followed by the activation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Apoptosis; Bax; Mitochondria; Endoplasmic reticulum; Neurodegeneration and aging.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300011

Attenuation of oxidative stress, inflammation and apoptosis by ethanolic and aqueous extracts of Crocus sativusL. stigma after chronic constriction injury of rats
Provedor de dados: 42
Autores: AMIN,BAHAREH; ABNOUS,KHALIL; MOTAMEDSHARIATY,VAHIDEH; HOSSEINZADEH,HOSSEIN.
In our previous study, the ethanolic and aqueous extracts of Crocus sativus elicited antinociceptive effects in the chronic constriction injury (CCI) model of neuropathic pain. In this study, we explored anti-inflammatory, anti-oxidant and anti-apoptotic effects of such extracts in CCI animals. A total of 72 animals were divided as vehicle-treated CCI rats, sham group, CCI animals treated with the effective dose of aqueous and ethanolic extracts (200 mg/kg, i.p.). The lumbar spinal cord levels of proinflammatory cytokines including tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and interleukin 6 (IL-6), were evaluated at days 3 and 7 after CCI (n=3, for each group). The apoptotic protein changes were evaluated at days 3 and 7 by western blotting....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crocus sativus; Chronic constriction injury; Inflammatory cytokines; Oxidative stress; Apoptosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401821

Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed
Provedor de dados: 45
Autores: Sprícigo,J.F.W.; Leme,L.O.; Guimarães,A.L.; Oliveira Neto,J.C.; Silva,P.C.P.; Moreira,N.H.; Pivato,I.; Silva,B.D.M.; Ramos,A.F.; Dode,M.A.N.
ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming, blastocysts were in vitro cultured to assess re-expansion and hatching at 24 and 48 hours. Finally, at 48 hours, hatched blastocysts were submitted to RT-qPCR searching for BCL2A1, BAK, BAX and CASP3 genes. For MALDI-TOF, the ion intensity was expressed in arbitrary units. Blastocyst development was compared by Qui-square (P&lt; 0.05). Among the most representative PL was the phosphatidylcholine [PC (32:0) + H]+; [PC (34:1) + H]+ and [PC (36:4) + H]+. Beyond the PL, MALDI revealed some triglycerides (TG), including PPL (50:2) + Na+, PPO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; Embryo; Cryopreservation; Lipids; Apoptosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300837

Morphometric analysis of the thymus of puppies infected with the Snyder Hill Strain of canine distemper virus
Provedor de dados: 45
Autores: Alves,C.M.; Vasconcelos,A.C.; Martins,A.S.; del Puerto,H.L.; Santos,F.G.A.; Nunes,J.E.S.; Campos,P.P.; Moro,L.
The thymic morphometry analysis was used for determining apoptosis and atrophy of the thymus of eight puppies inoculated with canine distemper virus (CDV). Three healthy dogs were used as negative controls. Sections, 5µm thick, were stained by HE and Shorr, and the latter were evaluated by morphometry. CDV nucleoprotein was detected by immunohistochemistry. Morphometric results confirmed lymphoid hypotrophy in CDV inoculated dog thymuses, more stroma, less parenchyma and higher apoptotic index/field than negative control (not inoculated) puppies. Apoptosis plays a role in the mechanism of thymus atrophy that develops in canine distemper.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puppy; Apoptosis; Thymus; Canine distemper; Immunology; Virus; Immunosuppression.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400004

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases
Provedor de dados: 45
Autores: Santos,F.G.A.; Vasconcelos,A.C.; Nunes,J.E.S.; Cassali,G.D.; Paixão,T.A.; Martins,A.S.; Silva,S.S.; Martins,R.F.; Moro,L.
Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Venereal tumor; Apoptosis; Cell death.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300013

Kint3-4 promotes apoptosis and inhibition of angiogenesis in solid Ehrlich Tumor
Provedor de dados: 45
Autores: Souza,C; Carvalho,L.F.; Ferreira,M.A.N.D; Pesquero,J.L.; Cassali,G.D.
A proteína Kint3-4 (PKint3-4), codificadora da angiostatina, é reconhecida por sua potencialidade antiangiogênica. O presente estudo teve como objetivo avaliar o efeito da proteína Kint3-4 no crescimento do tumor sólido de Ehrlich. Para isso, foram analisados a curva de desenvolvimento tumoral, o índice apoptótico e a dosagem de hemoglobina, a fim de se avaliar a angiogênese, em 20 camundongos Swiss fêmeas, inoculadas com o tumor sólido de Ehrlich em seus coxins plantares. Os resultados demonstraram a participação de PKint3-4 na indução à apoptose de células neoplásicas, na diminuição da concentração de hemoglobina e, principalmente, na diminuição do desenvolvimento tumoral. Sugere-se que a ação antitumoral, determinada pela sequência proteica utilizada,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Camundongo; Tumor de Ehrlich; Kint3-4; Apoptose; Angiogênese; Caspase-3; Tumor de Ehrlich; Mice; Ehrlich tumor; Kint3-4; Apoptosis; Angiogenesis; Caspase-3.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300036

Morphological abnormalities and apoptosis in lamellar tissue of equines after intestinal obstruction and treatment with hydrocortisone
Provedor de dados: 45
Autores: Laskoski,L.M; Valadão,C.A.A; Vasconcelos,R.O; Souza,A.H; Faleiros,R.R; Amorim,R.L.
Four experimental groups of equines were used in order to study morphological abnormalities and apoptosis in lamellar tissue. Group Cg (control) was composed of animals without any surgical procedure; group Ig (instrumented), animals that underwent enterotomy; group Tg (treated), animals that were subjected to intestinal obstruction and were treated with hydrocortisone; and group Ug (untreated), animals that were subjected to intestinal obstruction without treatment. The lamellar tissue was analyzed regarding the presence of tissue abnormalities and apoptosis. No morphological abnormalities were observed in animals of surgical groups, and no difference in apoptosis was observed between groups. It was concluded that intestinal obstruction allowed laminitis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Laminitis; Apoptosis; Intestinal obstruction; Morphological abnormalities.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600006

Determination of the minimal representative number of microscopic fields to quantify apoptosis in canine lymph nodes
Provedor de dados: 45
Autores: Moro,L.; Vasconcelos,A.C.; Santos,F.G.A.; Alves,C.M.; Nunes,J.E.S.; Sampaio,I.B.M.
Determinou-se o número mínimo de campos por lâmina para se ter uma amostragem representativa para o estudo de apoptose em linfonodos de cães com cinomose. Um linfonodo traqueobrônquico de cão foi colhido e processado segundo técnica para inclusão em parafina. Secções de 5µm foram coradas pelo tricrômico de Shorr. Utilizou-se um analisador de imagens para registrar o número de células em apoptose e os índices apoptóticos de 300 campos, na mesma lâmina. Obtiveram-se, então, valores médios para 10 amostras de 5, 10, 15, 20, 25, 30, 35, 40, 45 e 50 campos ao acaso. Realizou-se o estudo dos desvios-padrão das médias (DP) em relação ao tamanho da amostra que os originou. Quando as oscilações de DP entre amostras consecutivas foram menores que 5%, o ganho em...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dog; Apoptosis; Morphometry; Lymph node; Sampling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300020

Placental maturation and expulsion in Holstein and Nelore cows
Provedor de dados: 45
Autores: Martins,V.M.V.; Marques Junior,A.P.; Vasconcelos,A.C.; Martins,E.; Santos,R.L.; Lima,F.P.C.
Morphological changes in the placenta at periparturition and the involvement of apoptosis controlling cell populations in the placentome were evaluated in Holstein and Nelore cows. Twenty-two Holstein cows at the end of gestation with normal non-induced parturition (group I) and 10 Holstein cows with induced parturition (group II) were used in this study. In addition, groups III and IV were constituted by 10 Nelore cows at the end of gestation with normal non-induced parturition and 21 Nelore cows with induced parturition, respectivelly. Binucleate trophoblastic cells, epithelial caruncular cells and apoptotic bodies were quantified by light microscopy. Apoptosis was further evaluated by transmission electron microscopy, ELISA and DNA electrophoresis in...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bovine; Placentome; Binucleate cell; Epithelial cell; Apoptosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000200004

Etoposide-induced apoptosis in murine neuroblastoma (N2A) cells infected with Paramyxoviruses
Provedor de dados: 45
Autores: Moro,L.; Vasconcelos,A.C.; Martins,A.S.
The present study aimed to determine whether measles virus can induce apoptosis in murine neuroblastoma cells and the behavior of these cells under acute infection with measles virus or persistent infection with canine distemper virus upon treatment with etoposide. Measles virus induced necrosis in murine neuroblastoma cells. Canine distemper virus-persistent infection did not alter murine neuroblastoma cells behavior when treated with etoposide.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Measles virus; Canine distemper virus; Murine neuroblastoma cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000100007

Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
Provedor de dados: 45
Autores: Santos,W.G.; Silveira,T.L.; Fiúza,A.T.L.; Botelho,A.F.M.; Gonçalves,I.N.N.; Ferreira,E.; Soto-Blanco,B.; Melo,M.M.
RESUMO Este estudo objetiva reportar a ocorrência de apoptose in vivo induzida pelo veneno da serpente Bothrops alternatus em células musculares esqueléticas. Cinco coelhos machos, adultos, receberam 150μg/kg de veneno no músculo vasto lateral, enquanto outros cinco animais receberam 0,1% de BSA diluído em PBS no mesmo local. Após 12 horas, os animais foram eutanasiados, e amostras do local de inoculação foram coletadas para análise histopatológica. Foram evidenciadas necrose e hemorragia nas células musculares. Além disso, a análise imuno-histoquímica para identificação de caspase-3 ativada revelou marcações granulares e agregadas no citoplasma das células musculares, compatíveis com o processo de apoptose. Este é o primeiro relato que confirma o veneno...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snake venom; Botropic venom; Apoptosis; Toxicology; Rabbit.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100349

Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
Provedor de dados: 45
Autores: Sollecito,N.V.; Pereira,E.C.M.; Grázia,J.G.V.; Neves,B.P.; Couto,B.V.R.; Andrade,V.B.; Miranda,M.S.; Silva,J.K.R.; Borges,A.M.
ABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Apoptosis; Lippia origanoides; In vitro embryo production.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300723

Post-treatment with plant extracts used in Brazilian folk medicine caused a partial reversal of the antiproliferative effect of glyphosate in the Allium cepa test
Provedor de dados: 47
Autores: Dal-Souto Frescura,Viviane; Wouters Kuhn,Andrielle; Laughinghouse Iv,Haywood Dail; Terezinha Paranhos,Juçara; Bosio Tedesco,Solange.
Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared with the negative control (distilled water). Surprisingly, however, both extracts from P. brachypoda and P. birotula caused a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Psychotria brachypoda; Psychotria birotula; Cell proliferation; Apoptosis.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200002

Chronic stress effects on the apoptotic index of the adrenal cortex of pregnant rats
Provedor de dados: 47
Autores: Bozzo,Andrea; Soñez,Carlos Alberto; Mugnaini,María Teresa; Pastorino,Isabel Cecilia; Rolando,Alicia Nélida; Romanini,María Cristina; Gauna,Héctor Fernando.
Chronic stress by immobilization during gestation can alter several mechanisms that maintain homeostasis in adrenal gland. The aim of this work was to quantify the apoptotic index of adrenal cortex during mid-pregnancy and to prove cytological characteristics by electron microscopy. The apoptotic index did not present significant differences between the adrenal cortex areas of control and experimental rats in any of the three ages studied. The day of gestation influenced significantly on the apoptotic index in both groups. This index increased as gestation progressed. It may be concluded that chronic stress by immobilization might induce the increase of apoptotic index in adrenal cortex as gestation progresses which might be related variations of plasmatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic stress; Apoptosis; Adrenal cortex; Pregnancy.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300004

Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro
Provedor de dados: 47
Autores: Yoko,Iizuka; Hong,Samin; Yun Kim,Chan; Kab Kim,Seung; Je Seong,Gong.
Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 &amp;#956;M) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal uridine deoxynucleotidyl transferase nick end-labeling (TUNEL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuronal damage; Apoptosis; Neuroprotection.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300005

In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line
Provedor de dados: 47
Autores: Vorster,Chris; Joubert,Annie.
In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM's in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4µM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; 2-methoxyestradiol; Sulphamoylated estradiol derivative; Apoptosis; Autophagy.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200003

Cysteine proteinases of Trypanosoma cruzi: from digestive enzymes to programmed cell death
Provedor de dados: 47
Autores: Kosec,Gregor; Alvarez,Vanina; Cazzulo,Juan J.
Trypanosoma cruzi, the parasite causing Chagas disease, contains a number of proteolytic enzymes. The recent completion of the genome sequence of the T. cruzi CL Brener clone suggests the presence of 70 cysteine peptidases, 40 serine peptidases (none of them from the chymotrypsin family), about 250 metallopeptidases (most leishmanolysin homologues), 25 threonine peptidases, and only two aspartyl peptidases, none of them from the pepsin family. The cysteine peptidases belong to 7 families of Clan CA, 3 families of Clan CD, and one each of Clans CE and CF. In Clan CA, the C1 family is represented by cruzipains 1 and 2, biochemically well characterized, as well as cathepsin B and two other cathepsins. There are a number of homologues to calpains (family C2),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Chagas disease; Cysteine proteinases; Apoptosis; Autophagy.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300008

Influence of estradiol analogue on cell growth, morphology and death in esophageal carcinoma cells
Provedor de dados: 47
Autores: Mqoco,Thandi; Marais,Sumari; Joubert,Annie.
2-Methoxyestradiol-bis-sulphamate is a bis-sulphamoylated derivative of the naturally occurring 17-beta-estradiol metabolite namely 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate is regarded as a potential anticancer drug with increased antiproliferative activity when compared to 2-methoxyestradiol. The aim of this pilot in vitro study was to determine the influence of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and possible induction of certain types of cell death in the SNO esophageal carcinoma cell line. A dose-dependent study (0.2-1.0µM) was conducted with an exposure time of 24 hours. Data revealed that 2-methoxyestradiol-bis-sulphamate reduced cell numbers statistically significantly to 74% after exposure to 0.4µM of the drug....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Apoptosis; Autophagy.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300003

CAS role in the brain apoptosis of Bufo arenarum induced by Cypermethrin
Provedor de dados: 47
Autores: Izaguirre,M. F.; Vergara,M. N.; Casco,V. H.
CAS might have a key role in the apoptosis induced by toxins, acting as anti-apoptotic factor, stimulating the cellular proliferation and the cell contact stabilization. To start to elucidate their role in the brain apoptosis of Bufo arenarum induced by cypermethrin (CY), the expression patterns of CAS and several cell adhesion molecules (CAMs) were established. Bufo arenarum tadpoles of the control and acute bioassay survival at different doses (39, 156, 625 and 2,500 µg CY/L) and times (24, 48, 72 and 96 h) of CY treatment were fixed in Carnoy, embedded in paraffin and sectioned. CAS and CAMs expression was determined by immunofluorescence and immunohistochemistry, respectively. When the bioassay starts, CAS increases suggesting a proliferative or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Brain; Cypermethrin; CAS; Cams.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200007

WT-1 mRNA expression is modulated by nitric oxide availability and Hsp70 interaction after neonatal unilateral ureteral obstruction
Provedor de dados: 47
Autores: Mazzei,Luciana; García,Isabel Mercedes; Cacciamani,Valeria; Benardón,María Eugenia; Manucha,Walter.
Wilms tumor gene 1 (wt-1), a key regulator of mesenchymal-epithelial transformation, is downregulated during congenital obstructive nephropathy, leading to apoptosis. There is a functional interaction between WT-1 and inducible nitric oxide synthase (iNOS). In this regard, we reported that after neonatal unilateral ureteral obstruction, rosuvastatin prevents apoptosis through an increase in nitric oxide bioavailability, which in turn is linked to higher Hsp70 expression. Hence, the goal of this study was to determine whether a nitric oxide/Hsp70 interaction is involved in changes in WT-1 mRNA expression after ureteral obstruction. Neonatal rats submitted to experimental ureteral obstruction were treated with either vehicle or rosuvastatin for 14 days....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obstructive nephropathy; Apoptosis; Statins; Kidney development.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300004

Apoptosis as pathogenic mechanism of infection with vesicular stomatitis virus: Evidence in primary bovine fibroblast cultures
Provedor de dados: 47
Autores: López-Herrera,A.; Ruiz-Sáenz,J.; Góez,Y.P.; Zapata,W.; Velilla,P.A.; Arango,A.E.; Urcuqui-Inchima,S.
To determine whether fibroblasts from Blanco Orejinegro cattle, exhibit any level of resistance to infection against vesicular stomatitis virus (VSV) serotypes Indiana (VSV-I) or New Jersey (VSVNJ), 30 fibroblast cultures were phenotyped to evaluate their resistance/susceptibility. Thirty three % of Blanco Orejinegro fibroblast cultures were classified as very resistant, 50% as resistant, and 17% as susceptible to VSV-I infection, whereas 20% were classified as very resistant, 50% as resistant and 30% as susceptible to VSV-NJ infection. Therefore, there appears to be a large variation in phenotypic polymorphism among the fibroblasts to infection by VSV. To elucidate the mechanisms responsible for this diversity, we searched for a possible relationship...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vesicular stomatitis virus; Apoptosis; Resistance; Bovine fibroblasts (Source: NLM).
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200007

Mechanisms involved in the cytotoxic effects of berberine on human colon cancer HCT-8 cells
Provedor de dados: 47
Autores: XU,Li-na; LU,Bi-nan; HU,Ming-ming; XU,You-wei; HAN,Xu; QI,Yan; PENG,Jin-yong.
Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ethidium bromide staining. The concentrations of lactate dehydrogenase and both acid and alkaline phosphatases were significantly increased in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell cycle arrest; Death receptor pathway; Mitochondrial pathway; Natural product.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300003

Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat: A freeze-fracture study
Provedor de dados: 47
Autores: Cavicchia,Juan Carlos; Guembe,Gustavo; Fóscolo,Mabel.
In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Corpus luteum; Nuclear envelope; Nucleocytoplasmic transport.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200004

Mitochondrial apoptotic pathways
Provedor de dados: 47
Autores: Mohamad,Nora; Gutiérrez,Alicia; Núñez,Mariel; Cocca,Claudia; Martín,Gabriela; Cricco,Graciela; Medina,Vanina; Rivera,Elena; Bergoc,Rosa.
Apoptosis or programmed cell death (PCD) is a physiological process characteristic of pluricellular organisms leading to self-destruction of the cell. It is therefore involved in development, homeostasis and host defense. However, a significant difference has been shown between mammalian cell apoptosis and non-mammalian cell apoptosis: mitochondria are implicated only in the former. Execution of PCD includes the release of several proapoptotic proteins from the intermembrane space of mitochondria. They could exert their actions through a caspase dependent as well as a caspase independent way. On the other hand, regulation of PCD is mainly given by the Bcl-2 family members, which are in turn essentially regulated by activation of death receptors and/or DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Mitochondria; Bcl-2 family; Cytochrome-c.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200001

Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies
Provedor de dados: 47
Autores: Lopez,Lidia M.; Dubin,Marta; Carrizo,Patricia H.; Burgos,Mario H.; Pellegrino de Iraldi,Amanda; Stoppani,Andrés O. M.
CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho&amp;#091;1,2b&amp;#093;pyran- 5,6-dione; CG-NQ), a b-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; O-naphthoquinones; Hepatocytes; Chromatin Alteration; Mitochondrial Swelling Membrane; Blebs.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200007

Influence of 2-methoxyestradiol on MCF-7 cells: An improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels
Provedor de dados: 47
Autores: Joubert,Annie; Marais,Sumari; Maritz,Christine.
Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 &amp;#956;M 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Breast carcinoma; Mitosis; Apoptosis.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100007

The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells
Provedor de dados: 48
Autores: CARBALLO,JESÚS A; PINCHEIRA,JUANA; DE LA TORRE,CONSUELO.
Root growth, G2 length, and the frequency of aberrant mitoses and apoptotic nuclei were recorded after a single X-ray irradiation, ranging from 2.5 to 40 Gy, in Allium cepa L. root meristematic cells. After 72 h of recovery, root growth was reduced in a dose-dependent manner from 10 to 40 Gy, but not at 2.5 or 5 Gy doses. Flow cytometry plus TUNEL (TdT-mediated dUTP nick end labeling) showed that activation of apoptosis occurred only after 20 and 40 Gy of X-rays. Nevertheless, irrespective of the radiation dose, conventional flow cytometry showed that cells accumulated in G2 (4C DNA content). Simultaneously, the mitotic index fell, though a mitotic wave appeared later. Cell accumulation in G2 was transient and partially reversed by caffeine, thus it was...
Tipo: Journal article
Palavras-chave: Apoptosis; Checkpoint adaptation; G2 DNA damage checkpoint; Genome instability; X-rays.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200015

Ion movements in cell death: from protection to execution
Provedor de dados: 48
Autores: FELIPE BARROS,LUIS; CASTRO,JOEL; BITTNER,CARLA X.
Cell death is preceded by severe disruption of inorganic ion homeostasis. Seconds to minutes after an injury, calcium, protons, sodium, potassium and chloride are exchanged between the cell and its environment. Simultaneously, ions are shifted between membrane compartments inside the cell, whereby mitochondria and endoplasmic reticulum play a crucial role. Depending of the type and severity of injury, two mutually exclusive metastable states can be reached, which predict the final outcome. Cells characterized by large increases in cytosolic [Ca2+], [Na+]; and [Mg2+] swell and die by necrosis; alternatively, cells characterized by high [H+]and low [K+], with normal [Na+] and normal to moderate [Ca2+] increases die by apoptosis. The levels of these ions...
Tipo: Journal article
Palavras-chave: Cell death; Membrane channels; Apoptosis; Necrosis.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200012

Cobalt oxide nanoparticles aggravate DNA damage and cell death in eggplant via mitochondrial swelling and NO signaling pathway
Provedor de dados: 48
Autores: Faisal,Mohammad; Saquib,Quaiser; Alatar,Abdulrahman A.; Al-Khedhairy,Abdulaziz A.; Ahmed,Mukhtar; Ansari,Sabiha M.; Alwathnani,Hend A.; Dwivedi,Sourabh; Musarrat,Javed; Praveen,Shelly.
BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron...
Tipo: Journal article
Palavras-chave: Cobalt oxide nanoparticles; Nanotoxicity; DNA damage; Apoptosis; Oxidative stress.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100020

Expression of TRF2 and its prognostic relevance in advanced stage cervical cancer patients
Provedor de dados: 48
Autores: Ozden,Sevgi; Tiber,Pinar Mega; Ozgen,Zerrin; Ozyurt,Hazan; Serakinci,Nedime; Orun,Oya.
BACKGROUND: Telomeres are protective caps consisted of specific tandem repeats (5&amp;#8242;-TTAGGG-3&amp;#8242;). Shortening of telomeres at each cell division is known as "mitotic clock" of the cells, which renders telomeres as important regulators of lifespan. TRF2 is one of the critical members of shelterin complex, which is a protein complex responsible from the preservation of cap structure, and loss or mutation of TRF2 results in DNA damage, senescence or apoptosis. Since cancer is frequently associated with aberrant cell cycle progression, defective DNA repair or apoptosis pathways, TRF2 could be one likely candidate for cancer therapy. Here we investigated the prognostic role of TRF2 levels in cervical cancer patients. Fold-induction rates were...
Tipo: Journal article
Palavras-chave: Cervical cancer; Radiotherapy; Telomere repeat-binding factors 2; B-cell lymphoma-extra large; Apoptosis.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100055

Induction of programmed cell death by Prangos uloptera, a medicinal plant
Provedor de dados: 48
Autores: ZAHRI,SABER; RAZAVI,SEYED MEHDI; NIRI,FARSHAD HASSANZADEH; MOHAMMADI,SARIEH.
Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inhibitory and cell cytotoxicity effects of the extract were assayed by MTT and Trypan-blue tests, respectively. Morphological changes and DNA fragmentation were also evaluated. The viability tests showed 0.49 and 0.3 mg/ml as 50% inhibition concentration and 50% cytotoxicity concentration after 24 hours of treatment, respectively. Fluorescent microscopy analysis revealed chromatin fragmentation and scanning electron microscopy showed cell shrinkage and cytoplasmic...
Tipo: Journal article
Palavras-chave: Apoptosis; Cytotoxicity; McCoy; Prangos uloptera.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400013

Effects of silk sericin on the proliferation and apoptosis of colon cancer cells
Provedor de dados: 48
Autores: Kaewkorn,Waraporn; Limpeanchob,Nanteetip; Tiyaboonchai,Waree; Pongcharoen,Sutatip; Sutheerawattananonda,Manote.
Sericin is a silk protein woven from silkworm cocoons (Bombyx mori). In animal model, sericin has been reported to have anti-tumoral action against colon cancer. The mechanisms underlying the activity of sericin against cancer cells are not fully understood. The present study investigated the effects of sericin on human colorectal cancer SW480 cells compared to normal colonic mucosal FHC cells. Since the size of the sericin protein may be important for its activity, two ranges of molecular weight were tested. Sericin was found to decrease SW480 and FHC cell viability. The small sericin had higher anti-proliferative effects than that of the large sericin in both cell types. Increased apoptosis of SW480 cells is associated with increased caspase-3 activity...
Tipo: Journal article
Palavras-chave: Silk sericin; Colon cancer; Apoptosis; Bcl-2; Caspase-3.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100006

Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
Provedor de dados: 48
Autores: Guo,Jian-Ru; Chen,Qian-Qian; Lam,Christopher Wai-Kei; Zhang,Wei.
BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULTS: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin...
Tipo: Journal article
Palavras-chave: Karanjin; Cell cycle; Apoptosis; Cancer therapy.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040

Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells
Provedor de dados: 48
Autores: Doan,Chung Chinh; Le,Long Thanh; Hoang,Son Nghia; Do,Si Minh; Van Le,Dong.
BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, leads on restricting cancer progression. The purpose of this study is to examine the therapeutic potential of dual gene targeted siRNA cocktail on human hepatocellular carcinoma Hep3B cells. RESULTS: The predesigned siRNAs could inhibit VEGF and KSP at mRNA level. siRNA cocktail showed a further downregulation on KSP mRNA and protein levels compared to KSP-siRNA or VEGF-siRNA, but not on VEGF expression. It also exhibited greater suppression on cell...
Tipo: Journal article
Palavras-chave: Vascular endothelial cell growth factor (VEGF); Kinesin spindle protein (KSP); SiRNA cocktail; Proliferation; Apoptosis; Hepatocellular carcinoma.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100064

Free radical chemistry in biological systems
Provedor de dados: 48
Autores: VALDEZ,LAURA B; LORES ARNAIZ,SILVIA; BUSTAMANTE,JUANITA; ALVAREZ,SILVIA; COSTA,LIDIA E; BOVERIS,ALBERTO.
Mitochondria are an active source of the free radical superoxide (O2-) and nitric oxide (NO), whose production accounts for about 2% and 0.5% respectively, of mitochondrial O2 uptake under physiological conditions. Superoxide is produced by the auto-oxidation of the semiquinones of ubiquinol and the NADH dehydrogenase flavin and NO by the enzymatic action of the nitric oxide synthase of the inner mitochondrial membrane (mtNOS). Nitric oxide reversibly inhibits cytochrome oxidase activity in competition with O2. The balance between NO production and its utilization results in a NO intramitochondrial steady-state concentration of 20-50 nM, which regulates mitochondrial O2 uptake and energy supply. The regulation of cellular respiration and energy production...
Tipo: Journal article
Palavras-chave: Superoxide radical; Nitric oxide; Peroxynitrite; Mitochondria; Apoptosis; Aging.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200005

Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and Go/G1 phase arrest in human leukemia HL-60 cells
Provedor de dados: 48
Autores: Pieme,Constant Anatole; Santosh,Guru Kumar; Tekwu,Emmanuel Mouafo; Askun,Tülin; Aydeniz,Hatice; Ngogang,Jeanne Yonkeu; Bhushan,Shashi; Saxena,Ajit Kumar.
BACKGROUND: Zanthoxylum heitzii is a spice used to prepare several dishes and to treat tumors, syphilis, malaria, cardiac palpitations, urogenital infections in the west region of Cameroon, but the antitumor mechanisms and chemical composition are not yet investigated. This study was aimed to determine the antiproliferative effects of four extracts from the fruits and barks of Zanthoxyllum heitzii (Rutaceae) on apoptosis in human promyelocytic cells, their mechanisms and the chemical composition. The 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the fifty percent inhibition (IC50) concentration of the cell lines after treatment. The effect on morphology was observed using a light or fluorescence...
Tipo: Journal article
Palavras-chave: Zanthozyllum heitzii; Syringic acid; Apoptosis; HL-60 cells; ROS; Mitochondrial membrane potential.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100048

TMPYP4 exerted antitumor effects in human cervical cancer cells through activation of p38 mitogen-activated protein kinase
Provedor de dados: 48
Autores: Cheng,Ming&amp;#8209;Jun; Cao,Yun&amp;#8209;Gui.
Abstract  Background  The aim of the present study was to investigate the potential effects of the 5,10,15,20&amp;#8209;tetrakis (1&amp;#8209;meth&amp;#8209; ylpyridinium&amp;#8209;4&amp;#8209;yl) porphyrin (TMPyP4) on the proliferation and apoptosis of human cervical cancer cells and the underlying mechanisms by which TMPyP4 exerted its actions.  Results  After human cervical cancer cells were treated with different doses of TMPyP4, cell viability was determined by 3&amp;#8209;(4,5&amp;#8209;dimethyl&amp;#8209;2&amp;#8209;thiazolyl)&amp;#8209;2,5&amp;#8209;diphenyl&amp;#8209;2&amp;#8209;H&amp;#8209;tetrazolium bromide (MTT) method, the apoptosis was observed by flow cytometry (FCM), and the expression of p38 mitogen&amp;#8209;activated protein kinase...
Tipo: Journal article
Palavras-chave: TMPyP4; P38 MAPK; Human cervical cancer cells; Proliferation; Apoptosis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100217

Low concentrations of doxycycline attenuates FasL-induced apoptosis in HeLa cells
Provedor de dados: 48
Autores: Yoon,Jung Mi; Koppula,Sushruta; Huh,Se Jong; Hur,Sun Jin; Kim,Chan Gil.
BACKGROUND: Doxycycline (DC) has been shown to possess non-antibiotic properties including Fas/Fas Ligand (FasL)-mediated apoptosis against several tumor types in the concentration range of 10-40 &amp;#956;g/mL. However, the effect of DC in apoptotic signaling at much low concentrations was not studied. METHODS: The present study investigated the attenuation effect of low dose of DC on FasL-induced apoptosis in HeLa cell by the methods of MTT assay, fluorescence microscopy, DNA fragmentation, flow cytometry analysis, and western blotting. RESULTS AND CONCLUSION: In the present findings we showed that low concentration of DC (&lt;2.0 &amp;#956;g/mL) exhibited protective effects against FasL-induced apoptosis in HeLa cells. FasL treatment to HeLa cells...
Tipo: Journal article
Palavras-chave: Apoptosis; Caspase; Cisplatin; FasL; Hydrogen peroxide; Tetracycline.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100038

Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells
Provedor de dados: 48
Autores: Du,Maotao; Zhang,Zhong; Gao,Tao.
Abstract  Background Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells.  Methods Melanoma cell lines WM266-4 and A2058 were treated either with or without piceatannol. Cell viability and cell apoptosis were assessed by using MTT and Annexin V/PI assay, respectively. Cells were transfected with specific...
Tipo: Journal article
Palavras-chave: Piceatannol; Apoptosis; MicroRNA-181-a; Melanoma cells.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100226

Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis
Provedor de dados: 48
Autores: Wang,Hui; Liu,Zheng; Zhang,Wenxiu; Yuan,Ziao; Yuan,Hongyi; Liu,Xueting; Yang,Chunwen; Guan,Weijun.
BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 &amp;#956;&amp;#924;) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It...
Tipo: Journal article
Palavras-chave: Siberian tiger; Cadmium; Apoptosis; Fibroblast; Calcium.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100042

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
Provedor de dados: 48
Autores: Hernandez,Pedro P; Undurraga,Cristian; Gallardo,Viviana E; Mackenzie,Natalia; Allende,Miguel L; Reyes,Ariel E.
Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae...
Tipo: Journal article
Palavras-chave: Acute exposure; Apoptosis; Copper; Early life stage; Heat shock; Protein-70; Zebrafish.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100002

Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
Provedor de dados: 48
Autores: Urriola-Muñoz,Paulina; Lizama,Carlos; Lagos-Cabré,Raúl; Reyes,Juan G; Moreno,Ricardo D.
BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. RESULTS: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of...
Tipo: Journal article
Palavras-chave: Testis; Spermatogenesis; Apoptosis; Paracrine; Metalloproteinase.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100031

Phospholipid synthesis, diacylglycerol compartmentation, and apoptosis
Provedor de dados: 48
Autores: WRIGHT,MARCIA M.; MCMASTER,CHRISTOPHER R.
Apoptosis is a means by which organisms dispose of unwanted cells without inducing an inflammatory response. Alterations in apoptosis is a common process by which cells become cancerous. Paradoxically, many cancer chemotherapeutics preferentially kill cancer cells by inducing apoptosis. Diacylglycerol is a lipid second messenger that regulates cell growth and apoptosis and is produced during signal transduction by hydrolysis of membrane phospholipids. Protein kinase Cs are a family of diacylglycerol responsive enzymes that are recruited to cellular membranes as a consequence of diacylglycerol production where they phosphorylate specific target proteins responsible for regulating cell growth. In this review, we will first summarize our current understanding...
Tipo: Journal article
Palavras-chave: Apoptosis; Diacylglycerol; Farnesol; Phorbol ester; Protein kinase C.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200014

Mitochondrial Ca2+ homeostasis in health and disease
Provedor de dados: 48
Autores: CAMPANELLA,MICHELANGELO; PINTON,PAOLO; RIZZUTO,ROSARIO.
Although it has long been known that mitochondria possess a complex molecular repertoire for accumulating and releasing Ca2+, only in recent years has a large body of data demonstrated that these organelles promptly respond to Ca2+-mediated cell stimulations. In this contribution, we will review the principles of mitochondrial Ca2+ homeostasis and its signaling role in different physiological and pathological conditions.
Tipo: Journal article
Palavras-chave: Mitochondria; Calcium; Apoptosis; Bcl-2; Viral proteins.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400022

MicroRNA-98 inhibits the cell proliferation of human hypertrophic scar fibroblasts via targeting Col1A1
Provedor de dados: 48
Autores: Bi,Sheng; Chai,Linlin; Yuan,Xi; Cao,Chuan; Li,Shirong.
Abstract  Background  Hypertrophic scarring (HS) is a severe disease, and results from unusual wound healing. Col1A1 could promote the hypertrophic scar formation, and the expression of Col1A1 in HS tissue was markedly higher than that in the normal. In present study, we aimed to identify miRNAs as post-transcriptional regulators of Col1A1 in HS.  Methods  MicroRNA-98 was selected as the key miRNA comprised in HS. The mRNA levels of miR-98 in HS tissues and the matched normal skin tissues were determined by qRT-PCR. MTT and flow cytometry were used to determine the influence of miR-98 on cell proliferation and apoptosis of HSFBs, respectively. Col1A1 was found to be the target gene of miR-98 using luciferase reporter assay. Luciferase assay was performed...
Tipo: Journal article
Palavras-chave: MiR-98; Col1A1; Apoptosis; Proliferation; HSFBs.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100215

Anticancer properties and enhancement of therapeutic potential of cisplatin by leaf extract of Zanthoxylum armatum DC
Provedor de dados: 48
Autores: Singh,Thangjam Davis; Meitei,Heikrujam Thoihen; Sharma,Adhikarimayum Lakhikumar; Robinson,Asem; Singh,Lisam Shanjukumar; Singh,Thiyam Ramsing.
BACKGROUND: Clinical use of chemotherapeutic drug, cisplatin is limited by its toxicity and drug resistance. Therefore, efforts continue for the discovery of novel combination therapies with cisplatin, to increase efficacy and reduce its toxicity. Here, we screened 16 medicinal plant extracts from Northeast part of India and found that leaf extract of Zanthoxylum armatum DC. (ZALE) induced cytotoxicity as well as an effect on the increasing of the efficiency of chemotherapeutic drugs (cisplatin, mitomycin C and camptothecin). This work shows detail molecular mechanism of anti-cancer activity of ZALE and its potential for combined treatment regimens to enhance the apoptotic response of chemotherapeutic drugs. RESULTS: ZALE induced cytotoxicity, nuclear...
Tipo: Journal article
Palavras-chave: Zanthoxylum armatum; Apoptosis; ERK activation; Cisplatin sensitization; DNA damage.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100046

Microglia ­ astrocyte interaction in Alzheimer's disease: friends or foes for the nervous system?
Provedor de dados: 48
Autores: VON BERNHARDI,ROMMY; RAMIREZ,GIGLIOLA.
Brain glial cells secrete several molecules that can modulate the survival of neurons after various types of damage to the CNS. Activated microglia and astrocytes closely associate to amyloid plaques in Alzheimer Disease (AD). They could have a role in the neurotoxicity observed in AD because of the inflammatory reaction they generate. There is controversy regarding the individual part played by the different glial cells, and the interrelationships between them. Both astrocytes and microglia produce several cytokines involved in the inflammatory reaction. Moreover, the same cytokines may have different effects, depending on their concentration and the type of cells in the vicinity. In turn, the events occurring in response to injury may lead to changes in...
Tipo: Journal article
Palavras-chave: Apoptosis; SS-amyloid; Cell activation; Inflammatory process; Neurotoxicity; Senile plaques.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200017

Apoptosis in HEp-2 cells infected with Ureaplasma diversum
Provedor de dados: 48
Autores: Amorim,Aline Teixeira; Marques,Lucas Miranda; Santos,Angelita Maria Oliveira Gusmão; Martins,Hellen Braga; Barbosa,Maysa Santos; Rezende,Izadora Souza; Andrade,Ewerton Ferraz; Campos,Guilherme Barreto; Lobão,Tássia Neves; Cortez,Beatriz Araujo; Monezi,Telma Alvez; Machado-Santelli,Glaucia Maria; Timenetsky,Jorge.
BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and...
Tipo: Journal article
Palavras-chave: Ureaplasma diversum; Invasion; Apoptosis; HEp-2 cells.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100038

The proapoptotic activity of C-terminal domain of apoptosis-inducing factor (AIF) is separated from its N-terminal
Provedor de dados: 48
Autores: ZHANG,YONG; HAN,TAO; ZHU,QING; ZHANG,WEI; BAO,WEI; FU,HAI-JING; YANG,JIE; HUANG,XIAO-JUN; WEI,JUN-XIA; MENG,YAN-LING; ZHAO,JING; CAO,YUN-XIN; JIA,LIN-TAO; YANG,AN-GANG.
Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein that mediates both NADH-oxidizing and caspase-independent apoptosis. Further, the proapoptotic activity of AIF is located in the C-terminus of AIF, although the precise minimum sequence responsible for apoptosis induction remains to be investigated. In the present study, we generated two truncated AIFs, AIF&amp;#916;1-480-FLAG, which is a FLAG-tagged C-terminal peptide comprising amino acids from 481 to 613, and AIF360-480 containing amino acids from 360 to 480 of AIF. We used confocal microscopy to demonstrate that both the truncated proteins are expressed and located in the cytoplasm of transfected cells. AIF&amp;#916;1-480 but not AIF360-480 induces apoptosis in transfected cells. We also...
Tipo: Journal article
Palavras-chave: Apoptosis inducing factor (AIF); Apoptosis; Cytochrome c; Mitochondria; HER2.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200014

Galectin-8 promotes migration and proliferation and prevents apoptosis in U87 glioblastoma cells
Provedor de dados: 48
Autores: Metz,Claudia; Döger,Remziye; Riquelme,Elizabeth; Cortés,Priscilla; Holmes,Christopher; Shaughnessy,Ronan; Oyanadel,Claudia; Grabowski,Catalina; González,Alfonso; Soza,Andrea.
BACKGROUND: Glioblastoma is one of the most aggressive cancers of the brain. Malignant traits of glioblastoma cells include elevated migration, proliferation and survival capabilities. Galectins are unconventionally secreted glycan-binding proteins that modulate processes of cell adhesion, migration, proliferation and apoptosis by interacting with beta-galactosides of cell surface glycoproteins and the extracellular matrix. Galectin-8 is one of the galectins highly expressed in glioblastoma cells. It has a unique selectivity for terminally sialylated glycans recently found enhanced in these highly malignant cells. A previous study in glioblastoma cell lines reported that Gal-8 coating a plastic surface stimulates two-dimensional motility. Because in other...
Tipo: Journal article
Palavras-chave: Galectin-8; Glioblastoma; Cancer; Cell cycle; Apoptosis; Proliferation; Migration.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100033

Mycoplasma hyorhinis and Mycoplasma fermentans induce cell apoptosis and changes in gene expression profiles of 32D cells
Provedor de dados: 48
Autores: Liu,Wenbin; Shou,Chengchao.
Infection of mycoplasmas has been linked to various human diseases including arthritis, pneumonia, infertility and cancer. While Mycoplasma hyorhinis and Mycoplasma fermentans have been detected in gastric adenocarcinomas, the mechanisms underlyine the pathogenesis are unknown. In this study, cell growth kinetics, Hoechst 33258 staining, DNA ladder assays, Western blotting analysis and cDNA microarray assays were performed to investigate the roles of M. hyorhinis and M. fermentans during infection of mammalian cells. Our data demonstrated that these mycoplasmas inhibid the growth of immortalised cell lines (32D and COS-7) ane tumor cell lines (HeLa and AGS). In addition, the infection of the 32D cell line with M. hyorhinis and M. fermentans induced...
Tipo: Journal article
Palavras-chave: Mycoplasma; Apoptosis; Gene expression profiles.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400011

Protective effect of silk lutein on ultraviolet B-irradiated human keratinocytes
Provedor de dados: 48
Autores: Pongcharoen,Sutatip; Warnnissorn,Prateep; Lertkajornsin,Ongart; Limpeanchob,Nanteetip; Sutheerawattananonda,Manote.
Carotenoids are efficient antioxidants that are of great importance for human health. Lutein and zeaxanthin are carotinoids present in high concentrations in the human retina which are involved in the photoprotection of the human eye. Lutein may also protect the skin from ultraviolet (UV)-induced damage. The present study investigated the protective effect of lutein extracted from yellow silk cocoons of Bombyx mori on human keratinocytes against UVB irradiation. A human keratinocyte cell line and primary human keratinocytes were used to investigate the UVB protection effects of silk lutein and plant lutein. Silk lutein showed no cytotoxicity to keratinocytes. Treatment with silk lutein prior to UVB irradiation enhanced cell viability and cell...
Tipo: Journal article
Palavras-chave: Silk lutein; Keratinocytes; UV; Apoptosis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100006

Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae
Provedor de dados: 48
Autores: REYES,PAZ A; VARGAS,MACARENA F; GARCÍA,KATHERINE P; RUBILAR,PAULINA S; NAVARRETE,PATRICIA A; FUENTES,PAMELA M; RÍOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H; RUBIO,VÍCTOR H; HECKELS,JOHN; CHRISTODOULIDES,MYRON; CÁRDENAS,HUGO; VELASQUEZ,LUIS A.
Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-alpha dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of...
Tipo: Journal article
Palavras-chave: Apoptosis; Fallopian tube; Neisseria gonorrhoeae; Salpingitis; TNF-alpha.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400006

In vitro assessment of a computer-designed potential anticancer agent in cervical cancer cells
Provedor de dados: 48
Autores: Visagie,Michelle Helen; Jaiswal,Seema Rummurat; Joubert,Anna Margaretha.
BACKGROUND: Computer-based technology is becoming increasingly essential in biological research where drug discovery programs start with the identification of suitable drug targets. 2-Methoxyestradiol (2ME2) is a 17ß-estradiol metabolite that induces apoptosis in various cancer cell lines including cervical cancer, breast cancer and multiple myeloma. Owing to 2ME2's poor in vivo bioavailability, our laboratory in silico-designed and subsequently synthesized a novel 2ME2 analogue, 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10),15-tetraen-17-ol (ESE-15-ol), using receptor- and ligand molecular modeling. In this study, the biological effects of ESE-15-ol (180 nM) and its parent molecule, 2ME2 (1 &amp;#956;M), were assessed on morphology and apoptosis induction in...
Tipo: Journal article
Palavras-chave: Morphology; Apoptosis; Cervical cancer; ESE-15-ol.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100043

Propylthiouracil-induced hypothyroidism delays apoptosis during the first wave of spermatogenesis
Provedor de dados: 48
Autores: Silva,Doris; Lizama,Carlos; Tapia,Verónica; Moreno,Ricardo D.
Mammalian germ cell apoptosis plays a key role in controlling the correct number of germ cells supported by Sertoli cells during the first wave of spermatogenesis in mammalian puberty. However, little is known about hormonal factors that could influence the rate of germ cell apoptosis during puberty or adulthood. In this work we evaluate germ cell apoptosis under hypothyroidism induced by goitrogen propylthiouracil (PTU) during the first wave of spermatogenesis. Neonatally administered PTU promoted a delay in the differentiation of Sertoli cells as evaluated by the expression of clusterin using immunohistochemistry and RT-PCR. Clusterin had different expression levels in control and PTU-treated animals, but under both conditions the highest levels were...
Tipo: Journal article
Palavras-chave: Testis; Spermatocyte; Sertoli cell; Caspase; Apoptosis; Thyroid.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200010

The Role of Apollon Gene Silencing on Viablity and Radiosensitivity of Cervical Cancer Hela Cells
Provedor de dados: 52
Autores: Milani,Saeideh; Bandehpour,Mojgan; Sharifi,Zohreh; Kazemi,Bahram.
Cervical cancer is the second common cause of cancer deaths in women worldwide. Radioresistancy of cancer is a principal cause of treatment impairing. Inhibitor of apoptosis proteins (IAPs) widely block apoptosis against apoptotic stimuli, including current chemo- and radiation therapies. Apollon, a membrane of IAP, can support cells against apoptosis and is over expressed in some treatment-resistant cancer cells. The aim of this study was to evaluate the effects of apollon knockdown on induction of apoptosis and also its potential for enhancement of radiosensitvity on hela cells. plasmid encoding shRNA which has been confirmed its effect against apollon, transfected into hela cells. Consequent effects on the level of P53 , Bax and BAK analyzed by real...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apollon; Apoptosis; Radiosensitivity; Cervical cancer.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100320

Morphological alterations and G0/G1 cell cycle arrest induced by curcumin in human SK-MEL-37 melanoma cells
Provedor de dados: 52
Autores: Carneiro,Marcella Lemos Brettas; Porfírio,Elaine Paulucio; Otake,Andréia Hanada; Chammas,Roger; Báo,Sônia Nair; Guillo,Lidia Andreu.
The aim of this work was to study the effect of curcumin on cell cycle in the human SK-MEL-37 melanoma cell line. In addition, morphological and structural analyses were also performed. Flow cytometric analysis showed a G0/G1 arrest at 5 µM after 24 h exposure and a concentration-dependent increase in the proportion of sub-G0 hypodiploid cells. Typical apoptotic events were also observed by the fluorescence microscopy, transmission and scanning electronic microscopy. Loss of mitochondrial membrane potential was not detected. Results suggested that curcumin could arrest human melanoma cells at G0/G1 phase and induce a mitochondrial-independent apoptotic pathway.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin; Melanoma cells; Apoptosis; Cell cycle.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200013

Cardioprotection of Rosuvastatin Pre-conditioning on Myocardial Ischemia / Reperfusion Injury in a Rat Model
Provedor de dados: 52
Autores: Zhang,Pei-Xi; Zhang,Hong-Xia; Hou,Dai-Liang; Xiao,Zhi-Chao; Zhou,Cheng-Yun.
Abstract This study aimed to investigate the cardioprotection of rosuvastatin pre-conditioning (R-Pre) in a rat model of myocardial ischemia / reperfusion (I/R). Male SD rats were assigned into three groups: sham group, I/R group and R-Pre group. Rats in I/R group and R-Pre group received ischemia for 30 min and reperfusion for 2 h. In R-Pre group, rats received intragastrical administration with rosuvastatin at 5 mg/kg once daily for 1 week. After 2-h reperfusion, the cardiac function was detected by ultrasonography; the blood was collected for biochemical analysis; the heart was collected for the TUNEL staining and immunohistochemistry for Bcl-2 and Bax. Our results showed rosuvastatin pre-conditioning for 1 week could significantly reduce the infarct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial ischemia/reperfusion; Rosuvastatin; Oxidative stress; Apoptosis; Pre-conditioning.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100311

Apoptosis and Histopathology of the Heart after Renal Ischemia-Reperfusion in Male Rat Running title: Ischemia-Reperfusion Injury
Provedor de dados: 52
Autores: Alihemmati,Alireza; Yousefi,Hadi; Ahmadiasl,Naser; Habibi,Parisa.
ABSTRACT Ischemia-reperfusion injury was seen in strokes, myocardial infarctions, acute kidney injury, mesenteric ischemia, liver and systemic shock. Renal ischemia-reperfusion is more importance in the setting of kidney transplantation that affects distant organs. In this study forty Male Albino Wistar rats (200-250g) were randomly divided in four group (n=10) including control, sham operation group, nephrectomy and IRI group. All rats anesthetized with intraperitoneal injection of ketamine (50 mg/kg) and xylazine (10 mg/kg) and maintained the core body temperature at approximately 37°C. For inducing IRI group, it was performed right nephrectomy, and in continuing, the left kidney pedicle occluded to 45 min via nontraumatic microvascular clamp for making...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal Ischemia-reperfusion injury; Glycogen accumulation; Apoptosis; Heart; Rat.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100443

Aqueous extract of Huang-lian induces apoptosis in lung cancer cells via P53- mediated mitochondrial apoptosis
Provedor de dados: 52
Autores: Zhu,Deying; Lin,Zhe; Li,Zhenzhong.
The current study was designed to evaluate the activity of the aqueous extract of Huang-lian, and the main apoptosis pathway induced by the extracts of Huang-lian was detected on lung cancer. Antiproliferactive activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and TUNEL methods against human lung cancer cells (A549). Huang-lian regulated the Bcl-2 family protein-mediated mitochondrial pathway via p53 and was detected by western blot. It increased the activation of caspase-3 and caspase-3 cleavage increased as the time increased. These results suggested that Huang-lianregulated the Bcl-2 family protein-mediated mitochondrial via p53 pathway, suggesting that Huang-lian should be further investigation as a natural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Huang-lian; Aqueous extract; Apoptosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300353

Developmental Toxicity of (4S)-2- (4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic acid in Zebrafish ( Danio rerio )
Provedor de dados: 52
Autores: Akbulut,Cansu; Öztürk,Burcu; Genc,Hayriye; Zengin,Mustafa; Yön,Nazan Deniz.
ABSTRACT (4S)-2-(4-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid is new synthesized substance obtained from cysteine and valine. Thiazolidine derivates have important biological responses so scientists work intensively on these compounds in recent years. It is obvious that thiazolidine contained compounds will be used in future in the pharmaceutical industry to treat important diseases. Median lethal concentrations (LC50) for 48 h and 96 h were found as 1.106±0.052 mM and 0.804mM ± 0.102 respectively. According to LC50, exposure doses were determined as control, 0.4 mM, 0.2 mM and 0.1 mM (4S)-2-(4-hydroxy-3-methoxyphenyl)thiazolidine-4-carboxylic acid. Developmental toxicity and apoptotic features on zebrafish development were evaluated in this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thiazolidine; Toxicity; Development; Zebrafish; Apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100431

Evaluation of cell proliferation and apoptosis in placentas of rats with severe diabetes
Provedor de dados: 52
Autores: Rudge,Marilza Vieira Cunha; Costa,Elaine; Barbisan,Luis Fernando; Damasceno,Débora Cristina; Bueno,Aline; Saito,Felipe Hiroshi; Calderon,Iracema Mattos Paranhos; Rodrigues,Marcela Marcondes Pinto.
The aim of this work was to analyze the cell proliferation and apoptosis indexes on the 18th and 21st days of pregnancy of diabetic rats and to correlate with maternal glycemia and perinatal outcomes. Placentas from 20 Wistar rats were collected and divided into four experimental groups: control and diabetic of 18 and 21 days of pregnancy. The cell proliferation was analyzed using the PCNA expression and apoptosis by the TUNEL method. It was observed that PCNA and TUNEL indexes decreased from day 18 to 21 of pregnancy in the placentas of diabetic rats and these values were lower than control groups. Diabetic dams presented higher percentage of small for pregnancy age (SPA) fetuses. However, there was no difference between the PCNA and TUNEL indexes in SPA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Cell proliferation; Apoptosis; Placenta; Rat; IUGR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200009

Apoptosis induction by E2F decoy DNA of the prostate cancer cell line
Provedor de dados: 52
Autores: Wang,Tao; Jiang,An -li; Zhang,Jian-ye; Jing,Fei.
To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1 were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot. EMSA demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decoy DNA; Transcription factors; PC-3M cells; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200011

Aqueous Extract of Terminalia chebula Induces Apoptosis in Lung Cancer Cells Via a Mechanism Involving Mitochondria-mediated Pathways
Provedor de dados: 52
Autores: Wang,Meiling; Yang,Limin; Ji,Musi; Zhao,Pengwei; Sun,Peng; Bai,Ruixia; Tian,Yunpeng; Su,Liping; Li,Cunbao.
The current study was designed to evaluate the aqueous extract of Terminalia chebula activity, and the main pathway was detected on lung cancer by extracts of T. chebula. Aqueous extract of T. chebula was separated using a zeolite, and five fractions of T. chebula extract were obtained and analyzed by ultraviolet (UV) and infrared (IR) spectroscopy. Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) methods against human lung cancer (A549) and mouse lung cancer cell line LLC. T. chebula acts by regulating the Bcl-2 family protein-mediated mitochondrial pathway detected by western blot. Fraction 4 of the T. chebula extract showed much function and was thus studied further. Fraction 4 increased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T. chebula; Aqueous extract; Infrared spectroscopy; Apoptosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200208

Crucial Residues Modulating Interface of hBcl-B - hBaxBH3 Heterodimer as Probed by Computational Methods
Provedor de dados: 52
Autores: Sivakumar,Dakshinamurthy; Sivaraman,Thirunavukkarasu.
ABSTRACT Cancerous cells develop resistance to cell death by over expression of anti-apoptotic proteins, which are specific to interact with pro-apoptotic and BH3-only proteins of Bcl-2 family. Delineating crucial residues mediating the heterodimer complexes (anti-apoptotic proteins - pro-apoptotic/BH3-only proteins) is indispensable to develop specific antagonists to anti-apoptotic proteins. In these backgrounds, we have herein reported crucial residues of hBaxBH3 and hBcl-B (an anti-apoptotic protein specifically interacts with human Bax but does not interact with human Bak) for hetero dimerization of the polypeptides and as well validated the structural determinants of the polypeptides through variety of virtual 'alanine mutants' and 'switch mutants' by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Anti-apoptotic proteins; BH3-only peptides; Dimer interface; Dockings; Virtual mutants.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100422

Apoptosis as a prognostic marker in canine mammary tumors by TUNEL
Provedor de dados: 62
Autores: Pereira,Kariny Sanches; Zuccari,Débora Aparecida Pires de Campos; Cury,Patrícia Maluf; Cordeiro,José Antônio.
Apoptosis plays an important role in oncogenesis determining tumor growth and aggressiveness. The aim of this study was to examine the relationship between apoptosis, diagnosis and prognosis in canine mammary tumors. Thirty bitches were submitted to tumor extirpation and a method of labeling apoptotic cells, that labels DNA fragments, by terminal deoxynucleotidyl-tranferase (TdT) mediated by 5' deoxy-uridine-triphosphate (dUTP) nick and labeling (TUNEL) was used. The apoptotic cells were counted in ten high power fields (10HPF). Statistical results showed that when there are more than 30 apoptotic cells/10HPF the prognosis is worse (p= 0,0005). Thus suggesting that apoptosis can be used as a prognostic marker in canine breast tumors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Prognosis; Breast cancer; Bitch.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000500007

Pathogenic and opportunistic respiratory bacteria-induced apoptosis
Provedor de dados: 55
Autores: Lancellotti,Marcelo; Pereira,Rafaella Fabiana Carneiro; Cury,Gisele Gentile; Hollanda,Luciana Maria de.
Several pathogenic or opportunistic bacteria have the ability to either induce or inhibit host cell apoptosis. The capacity to modulate cell pathways that result in the induction or delay of host cell apoptosis is considered to be an important bacterial virulence mechanism. These processes could be mediated by different host cell signaling pathways that are subverted by the bacteria. Pathogens are able to activate apoptotic proteins, such as caspases, or inactivate anti-apoptotic proteins, such as NFkB and the MAPKKs, or even up-regulate the endogenous receptor/ligand system that induces apoptosis, generally when the bacteria are bound to the host cell surface. The bacteria-induced apoptotic or anti-apoptotic processes are often related with the fact that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenic bacteria; Opportunistic bacteria; Apoptosis; Bacteria induce apoptosis; Respiratory bacteria.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300014

Apoptosis and progression of hepatic fibrosis in hepatitis C patients
Provedor de dados: 55
Autores: Schinoni,Maria Isabel; Paraná,Raymundo; Cavalcante,Daniel.
Hepatitis C is a worldwide endemic disease, affecting roughly 200 million people. It has a variable prognosis, depending on the progression to fibrosis. During the last five years, the importance of apoptosis for the pathogenesis of various diseases, including hepatitis, has been recognized. It has been suggested that an increase in T cell-apoptosis during a hepatitis C virus infection is the cause of impaired regulation of the immune cellular response, helping to maintain infection. Thus, the interest in discovering the probable mechanisms by which the hepatitis C virus perpetuates in the liver, and to determine the conditions that predispose for progression of this disease, makes investigation of apoptosis in hepatic injury of great interest. We have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Hepatitis C; Liver fibrosis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200009

Effects of Epstein-Barr virus on the development of dendritic cells derived from cord blood monocytes: an essential role for apoptosis
Provedor de dados: 55
Autores: Wang,Juan-Juan; Li,Yu-Feng; Jin,Ying-Ying; Wang,Xi; Chen,Tong-Xin.
OBJECTIVE: Epstein-Barr virus (EBV) is a ubiquitous human γ-herpes virus, which can adapt and evade host immune defense. Dendritic cells (DCs) play a pivotal role in the initiation and maintenance of immune responses. This study investigated the effects of EBV on cord blood monocytes derived DCs (CBDC). METHODS: Monocytes were isolated from cord blood and cultured in medium containing recombinant IL-4 and GM-CSF to induce DCs development. B95-8 supernatant was added in monocytes culture medium for EBV infection at day 0. Phenotypic characterization of DCs, apoptotic cells, and mitochondrial membrane potential (MMP) were detected by flow cytometry. The morphology was observed by Hoechst 33258 staining and TUNEL staining, the expression of X-linked inhibitor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendritic cells; Apoptosis; Epstein-Barr virus infections.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000100004

Leishmania donovani infection activates Toll-like receptor 2, 4 expressions and Transforming growth factor-beta mediated apoptosis in renal tissues
Provedor de dados: 55
Autores: Kumar,Vinod; Tiwari,Neeraj; Gedda,Mallikarjuna Rao; Haque,Rizwanul; Singh,Rakesh K.
Abstract The present study was aimed to identify the underlying mechanisms of improper renal function in Leishmania donovani infection that causes VL. Mice (BALB/c) were infected with L. donovani and different parameters for proteinuria were assessed. The levels of superoxide anion (O2 -), hydrogen peroxide (H2O2), lipid peroxidation (MDA), inflammatory cytokines, and toll-like receptor (TLR) 2 and 4 expression were found significantly elevated at 60th day in these animals and declined at 90th day post infection. However, TGF-β and caspase 3 activities were higher at 90th day in comparison to 60th day post infection. These findings suggested that exacerbated inflammatory conditions correlate with abnormal renal functions in L. donovani infection, which is...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leishmaniasis; Renal; TLRs; Apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500545

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection
Provedor de dados: 55
Autores: Palma,Priscila F.R.; Baggio,Giovana L.; Spada,Celso; Silva,Renata da; Ferreira,Silvia Inês A.C.P.; Treitinger,Aricio.
Evaluation of apoptosis by flow cytometry is generally accomplished by methods that use annexin V-FITC as vital dye, which access phosphatidylserine exposed on the external membrane at the beginning of this process. In addition, the concomitant use of propidium iodide makes possible to verify the characteristic nuclear alterations in the late stages of apoptosis, as a consequence of the increase in membrane permeability. On the other hand, the use of calcein-AM in association with ethidium homodimer (EthD-1) allows the evaluation of cell apoptosis through detection of esterase activity and cellular membrane physical and chemical alterations. The aim of this study was to compare the sensibility of calcein-AM and EthD-1 with annexin V-FITC and propidium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Flow cytometry; Annexin V; Propidium iodide; Calcein-AM; Ethidium homodimer.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200003

Effect of N-acetyl-L-cysteine on lymphocyte apoptosis, lymphocyte viability, TNF-alpha and IL-8 in HIV-infected patients undergoing anti-retroviral treatment
Provedor de dados: 55
Autores: Treitinger,Aricio; Spada,Celso; Masokawa,Ivete Yoshico; Verdi,Júlio César Vidal; Silveira,Mariette Van Der  Sander; Luis,Magali Chaves; Reis,Marcellus; Ferreira,Silvia Inês Alejandra Cordova de Pires; Abdalla,Dulcinéia Saes Parra.
N-acetyl-L-cysteine (NAC) has been proposed as an additional therapeutic agent for AIDS patients because it reduces human immunodeficiency virus type 1 (HIV-1) replication in stimulated CD4+ lymphocytes, and it ameliorates immunological reactivity. In a randomized, 180-day, double-blind, placebo-controlled trial performed with HIV-infected patients classified as A2 and A3 according to the criteria of the Center for Disease Control and Prevention, we investigated the effects of oral administration of NAC on HIV-infected patients undergoing their first anti-retroviral therapy; viral load, CD4+ lymphocyte, lymphocyte viability and apoptosis, and TNF-alpha and IL-8 levels were determined. Sixteen patients who received anti-retroviral therapy plus a placebo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N-acetylcysteine; Apoptosis; Viability; HIV; Glutathione; TNF-alpha; IL-8.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500005

Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
Provedor de dados: 55
Autores: Khademvatan,Shahram; Gharavi,Mohammad Javad; Saki,Jasem.
OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. MATERIALS AND METHODS: Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Apoptosis; Gene expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500005

Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury
Provedor de dados: 56
Autores: Zhang,Weiwei; Li,Ying; Wang,Peng.
Long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of cardiovascular diseases, especially in myocardial infarction and ischemia/reperfusion (I/R). However, the underlying molecular mechanism remains unclear. In this study, we determined the role and the possible underlying molecular mechanism of lncRNA-ROR in myocardial I/R injury. H9c2 cells and human cardiomyocytes (HCM) were subjected to either hypoxia/reoxygenation (H/R), I/R or normal conditions (normoxia). The expression levels of lncRNA-ROR were detected in serum of myocardial I/R injury patients, H9c2 cells, and HCM by qRT-PCR. Then, levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were measured by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA; Ischemia/reperfusion (I/R); Hypoxia/reoxygenation (H/R); Cell viability; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600609

Benzyl isothiocyanate inhibits invasion and induces apoptosis via reducing S100A4 expression and increases PUMA expression in oral squamous cell carcinoma cells
Provedor de dados: 56
Autores: Ma,Lei; Chen,Yongjun; Han,Rui; Wang,Shuangyi.
Benzyl isothiocyanate (BITC) has been shown to inhibit invasion and induce apoptosis of various types of cancer. However, its role on human oral squamous cell carcinoma (OSCC) cells is still not well elucidated. In the present study, we investigated the effect of BITC on apoptosis and invasion of SCC9 cells, and its underlying mechanisms in vitro and in vivo. SCC9 cells were exposed to BITC (5 and 25 μM) for 24 and 48 h. Cell growth, apoptosis, invasion, and migration were detected in vitro by MTT, FITC-conjugated annexin V/propidium iodide staining followed by flow cytometry, Matrigel-coated semi-permeable modified Boyden, and wound-healing assay. S100A4, PUMA, and MMP-9 expressions were detected to investigate its mechanisms. Xenotransplantation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral squamous cell carcinoma; Benzyl isothiocyanate; Apoptosis; S100A4; MMP-9; PUMA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400605

Interleukin-27 augments the inhibitory effects of sorafenib on bladder cancer cells
Provedor de dados: 56
Autores: Cao,J.Y.; Yin,H.S.; Li,H.S.; Yu,X.Q.; Han,X.
Both sorafenib and interleukin-27 (IL-27) are antineoplastic drugs. This study aimed to investigate the synergistic effect of these two drugs on bladder cancer cells. HTB-9 and T24 cells were stimulated with IL-27 (50 ng/mL), sorafenib (2 μM) or the synergistic action of these two drugs. The cells without treatment acted as control. Cell proliferation, apoptosis and invasion were measured by bromodeoxyuridine assay, flow cytometry and modified Boyden chamber, respectively. Simultaneously, both modified Boyden chamber and scratch assay were used to assess cell migration. Finally, the phosphorylation levels of key kinases in the Akt/mechanistic target of rapamycin (mTOR)/mitogen-activated protein kinase (MAPK) pathway, and expression levels of matrix...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorafenib; Interleukin 27; Proliferation; Apoptosis; Invasion; Akt/mTOR/MAPK.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800610

Effect of different durations of overdistention on rat bladder function and morphology
Provedor de dados: 56
Autores: Jin,Xiao-Dong; Cao,Min; Zhou,Xie-Lai; Yin,Hong-Ping; Chen,Zhao-Dian; Xu,Ning; Jiang,Hai.
We investigated the contribution of the duration of overdistention (DOD) to rat bladder function and morphology and explored its possible molecular mechanisms. Bladder overdistention was induced in male Sprague-Dawley rats (200-250 g) by an infusion of saline. Forty rats were divided into 5 groups submitted to different DOD, i.e., 1, 2, 4, and 8 h, and control. Bladder function was evaluated by cystometry. Morphological changes were observed by light and transmission electron microscopy. Compared to control (44.567 ± 3.472 cmH2O), the maximum detrusor pressure of groups with 2-, 4- and 8-h DOD decreased significantly (means ± SEM): 32.774 ± 3.726, 31.321 ± 2.847, and 29.238 ± 3.724 cmH2O. With the increase of DOD, inflammatory infiltration and impairment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute urinary retention; Bladder overdistention; Urodynamics; Ultrastructure; Apoptosis; Rat.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000006

microRNA-142-3p inhibits apoptosis and inflammation induced by bleomycin through down-regulation of Cox-2 in MLE-12 cells
Provedor de dados: 56
Autores: Guo,F.; Lin,S.C.; Zhao,M.S.; Yu,B.; Li,X.Y.; Gao,Q.; Lin,D.J.
microRNA (miR)-142-3p is implicated in malignancy and has been identified as a biomarker for aggressive and recurrent lung adenocarcinomas. This study aimed to evaluate the inhibitory effect of miR-142-3p on apoptosis and inflammation induced by bleomycin in MLE-12 cells. MLE-12 cells were first transfected either with miR-142-3p mimic or miR-142-3p inhibitor and then the cells were exposed to 50 μg/mL of bleomycin. Thereafter, cell viability, apoptosis and the expression of pro-inflammatory cytokines were assessed using CCK-8, flow cytometry, RT-PCR and western blot analyses. Cox-2, PI3K, AKT and mTOR expressions were detected by western blotting after bleomycin was administered together with NS-398 (an inhibitor of Cox-2). As a result, cell viability was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-142-3p; MLE-12 cells; Bleomycin; Apoptosis; Pro-inflammatory cytokines; Idiopathic pulmonary fibrosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700606

The role of stress-activated protein kinase signaling in renal pathophysiology
Provedor de dados: 56
Autores: Ma,F.Y.; Liu,J.; Nikolic-Paterson,D.J.
Two major stress-activated protein kinases are the p38 mitogen-activated protein kinase (MAPK) and the c-Jun amino terminal kinase (JNK). p38 and JNK are widely expressed in different cell types in various tissues and can be activated by a diverse range of stimuli. Signaling through p38 and JNK is critical for embryonic development. In adult kidney, p38 and JNK signaling is evident in a restricted pattern suggesting a normal physiological role. Marked activation of both p38 and JNK pathways occurs in human renal disease, including glomerulonephritis, diabetic nephropathy and acute renal failure. Administration of small molecule inhibitors of p38 and JNK has been shown to provide protection from renal injury in different types of experimental kidney disease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P38; C-Jun amino terminal kinase; Inflammation; Fibrosis; Apoptosis; Renal pathophysiology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100006

Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage
Provedor de dados: 56
Autores: Ni,X.J.; Xu,Z.Q.; Jin,H.; Zheng,S.L.; Cai,Y.; Wang,J.J.
Ginsenoside Rg1, one of the most notable active components of Panax ginseng, has been widely reported to exert anti-inflammatory actions. This study aimed to reveal whether ginsenoside Rg1 also exhibits beneficial roles against lipopolysaccharide (LPS)-induced apoptosis and inflammation in human renal tubular epithelial cells, and to evaluate the potential role of the component on tubulointerstitial nephritis treatment. HK-2 cells were treated with various doses of ginsenoside Rg1 (0, 50, 100, 150, and 200 μM) in the absence or presence of 5 μg/mL LPS. Thereafter, CCK-8 assay, flow cytometry, western blot, migration assay, reactive oxygen species (ROS) assay, and ELISA were carried out to respectively assess cell viability, apoptosis, migration, ROS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginsenoside Rg1; LPS; Apoptosis; Inflammation; PI3K/AKT pathway; NF-κB pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200608

The inhibitory effect of photodynamic therapy and of an anti-VCAM-1 monoclonal antibody on the in vivo growth of C6 glioma xenografts
Provedor de dados: 56
Autores: Zhan,Qi; Yue,Wu; Shaoshan,Hu.
We investigated the effect of photodynamic therapy (PDT) and of an anti-vascular cell adhesion molecule-1 (VCAM-1) monoclonal antibody on the in vivo growth of C6 glioma. Seven days after inoculation with C6 cells, adult male Wistar rats weighing 280-300 g with MRI-confirmed glioma were randomly assigned to 4 groups (N = 15 per group): PDT + VCAM-1 antibody group; PDT group; VCAM-1 antibody group; control group. Eight days after inoculation, hematoporphyrin monomethyl ether (HMME) was administered as a photosensitizer and PDT was performed at 630 nm (illumination intensity: 360 J/cm²) for 10 min. VCAM-1 antibody (50 µg/mL) was then administered (0.5 mL) through the tail vein every other day from day 8 to day 16. At day 21, 5 rats in each group were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C6 glioma cells; Photodynamic therapy; Hematoporphyrin monomethyl ether; Anti-VCAM-1 monoclonal antibody; Apoptosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500016

A DNA enzyme targeting Egr-1 inhibits rat vascular smooth muscle cell proliferation by down-regulation of cyclin D1 and TGF-β1
Provedor de dados: 56
Autores: Wu,Y.; Han,W.; Liu,G.-N.
We have demonstrated that a synthetic DNA enzyme targeting early growth response factor-1 (Egr-1) can inhibit neointimal hyperplasia following vascular injury. However, the detailed mechanism of this inhibition is not known. Thus, the objective of the present study was to further investigate potential inhibitory mechanisms. Catalytic DNA (ED5) and scrambled control DNA enzyme (ED5SCR) were synthesized and transfected into primary cultures of rat vascular smooth muscle cells (VSMCs). VSMC proliferation and DNA synthesis were analyzed by the MTT method and BrdU staining, respectively. Egr-1, TGF-β1, p53, p21, Bax, and cyclin D1 expression was detected by RT-PCR and Western blot. Apoptosis and cell cycle assays were performed by FACS. Green fluorescence could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA enzyme; Egr-1; VSMC; Proliferation; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100004

Galectins: a key intersection between glycobiology and immunology
Provedor de dados: 56
Autores: Rabinovich,G.A.; Riera,C.M.; Landa,C.A.; Sotomayor,C.E.
Galectins are a family of evolutionarily conserved animal lectins, widely distributed from lower invertebrates to mammals. They share sequence and structure similarities in the carbohydrate recognition domain and specificity for polylactosamine-enriched glycoconjugates. In the last few years significant experimental data have been accumulated concerning their participation in different biological processes requiring carbohydrate recognition such as cell adhesion, cell growth regulation, inflammation, immunomodulation, apoptosis and metastasis. In the present review we will discuss some exciting questions and advances in galectin research, highlighting the significance of these proteins in immunological processes and their implications in biomedical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galectins; Immunomodulation; Apoptosis; Adhesion; Embryogenesis; Metastasis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400002

Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines
Provedor de dados: 56
Autores: Zong,L.; Yu,Q.H.; Du,Y.X.; Deng,X.M.
Studies have shown that edaravone may prevent liver injury. This study aimed to investigate the effects of edaravone on the liver injury induced by D-galactosamine (GalN) and lipopolysaccharide (LPS) in female BALB/c mice. Edaravone was injected into mice 30 min before and 4 h after GalN/LPS injection. The survival rate was determined within the first 24 h. Animals were killed 8 h after GalN/LPS injection, and liver injury was biochemically and histologically assessed. Hepatocyte apoptosis was measured by TUNEL staining; proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] in the liver were assayed by ELISA; expression of caspase-8 and caspase-3 proteins was detected by Western blot assay; and caspase-3 activity was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edaravone; Apoptosis; Endotoxin; Liver injury; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300231

Caspase dependence of the death of neonatal retinal ganglion cells induced by axon damage and induction of autophagy as a survival mechanism
Provedor de dados: 56
Autores: Sternberg,C.; Benchimol,M.; Linden,R.
We examined the degeneration of post-mitotic ganglion cells in ex-vivo neonatal retinal explants following axon damage. Ultrastructural features of both apoptosis and autophagy were detected. Degenerating cells reacted with antibodies specific for activated caspase-3 or -9, consistent with the presence of caspase activity. Furthermore, peptidic inhibitors of caspase-9, -6 or -3 prevented cell death (100 µM Ac-LEDH-CHO, 50 µM Ac-VEID-CHO and 10 µM Z-DEVD-fmk, respectively). Interestingly, inhibition of autophagy by 7-10 mM 3-methyl-adenine increased the rate of cell death. Immunohistochemistry data, caspase activation and caspase inhibition data suggest that axotomy of neonatal retinal ganglion cells triggers the intrinsic apoptotic pathway, which, in turn,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Autophagy; Caspases; Retina; Central nervous system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000005

Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a
Provedor de dados: 56
Autores: Fang,M.; Jin,A.; Zhao,Y.; Liu,X.
High plasma levels of homocysteine (Hcy) promote the progression of neurodegenerative diseases. However, the mechanism by which Hcy mediates neurotoxicity has not been elucidated. We observed that upon incubation with Hcy, the viability of a neuroblastoma cell line Neuro2a declined in a dose-dependent manner, and apoptosis was induced within 48 h. The median effective concentration (EC50) of Hcy was approximately 5 mM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) nuclear translocation and acylation has been implicated in the regulation of apoptosis. We found that nuclear translocation and acetylation of GAPDH increased in the presence of 5 mM Hcy and that higher levels of acetyltransferase p300/CBP were detected in Neuro2a cells. These findings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homocysteine; Glyceraldehyde-3-phosphate dehydrogenase; Apoptosis; Acetylate; P300/CBP.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200606

MicroRNA-455 suppresses the oncogenic function of HDAC2 in human colorectal cancer
Provedor de dados: 56
Autores: Mao,Q.D.; Zhang,W.; Zhao,K.; Cao,B.; Yuan,H.; Wei,L.Z.; Song,M.Q.; Liu,X.S.
Colorectal cancer (CRC) is the fourth leading cause of cancer-induced mortality. Histone deacetylase 2 (HDAC2) is involved in prognosis and therapy of CRC. This study aimed to explore novel therapeutic targets for CRC. The alteration of HDAC2 expression in CRC tissues was estimated by qRT-PCR. After lentivirus transfection, HDAC2 knockdown was confirmed by western blot analysis. The effect of HDAC2 knockdown on cell proliferation was then assessed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Screened by TargetScan, microRNA (miR)-455 was predicted to bind to 3′UTR of HDAC2 and the prediction was verified by luciferase assay. Finally, cells were transfected, respectively, with miR-455 mimics or miR-455 negative control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal cancer; Histone deacetylase 2; MiR-455; Proliferation; Apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600608

Side effects of oxysterols: cytotoxicity, oxidation, inflammation, and phospholipidosis
Provedor de dados: 56
Autores: Vejux,A.; Malvitte,L.; Lizard,G.
Oxysterols are 27-carbon atom molecules resulting from autoxidation or enzymatic oxidation of cholesterol. They are present in numerous foodstuffs and have been demonstrated to be present at increased levels in the plasma of patients with cardiovascular diseases and in atherosclerotic lesions. Thus, their role in lipid disorders is widely suspected, and they might also be involved in important degenerative diseases such as Alzheimer's disease, osteoporosis, and age-related macular degeneration. Since atherosclerosis is associated with the presence of apoptotic cells and with oxidative and inflammatory processes, the ability of some oxysterols, especially 7-ketocholesterol and 7β-hydroxycholesterol, to trigger cell death, activate inflammation, and modulate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Atherosclerosis; Inflammation; Oxysterols; Phospholipidosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700001

Propofol inhibits invasion and growth of ovarian cancer cells via regulating miR-9/NF-κB signal
Provedor de dados: 56
Autores: Huang,X.; Teng,Y.; Yang,H.; Ma,J.
Propofol is one of the most commonly used intravenous anesthetic agents during cancer resection surgery. A previous study has found that propofol can inhibit invasion and induce apoptosis of ovarian cancer cells. However, the underlying mechanisms are not known. miR-9 has been reported to be little expressed in ovarian cancer cells, which has been related to a poor prognosis in patients with ovarian cancer. Studies have also demonstrated that propofol could induce microRNAs expression and suppress NF-κB activation in some situations. In the present study, we assessed whether propofol inhibits invasion and induces apoptosis of ovarian cancer cells by miR-9/NF-κB signaling. Ovarian cancer ES-2 cells were transfected with anti-miR-9 or p65 cDNA or p65 siRNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; Ovarian cancer; MiR-9; NF-κB; Apoptosis; Invasion.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200605

Mechanistic effect of the human GJB6 gene and its mutations in HaCaT cell proliferation and apoptosis
Provedor de dados: 56
Autores: Lu,Yuting; Zhang,Ruili; Wang,Zhenying; Zhou,Shuhua; Song,Yali; Chen,Lamei; Chen,Nan; Liu,Wenmin; Ji,Canan; Wu,Wangli; Zhang,Li.
We constructed lentiviral vectors containing the human wild-type GJB6 gene and the mutant variants A88V and G11R. The three proteins were stably expressed by the Tet-on system in the HaCaT cell line and used to study the functional effect of the variants. The CCK-8 assay and flow cytometric analyses were used to determine the levels of cell proliferation and apoptosis. Western blot analyses were performed to analyze the relevant clinical indicators of hidrotic ectodermal dysplasia and markers of apoptosis in transfected HaCaT cells. The CCK8 assay and the flow cytometry results showed a significant increase (P&lt;0.05) in the apoptosis of HaCaT cells expressing the A88V and G11R mutants. In addition, we demonstrated that the A88V and G11R mutants induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clouston syndrome; GJB6 gene; Connexin 30; Caspase; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900611

MicroRNA-122 regulates caspase-8 and promotes the apoptosis of mouse cardiomyocytes
Provedor de dados: 56
Autores: Zhang,Z.W.; Li,H.; Chen,S.S.; Li,Y.; Cui,Z.Y.; Ma,J.
Cardiomyocyte apoptosis plays key roles in the pathogenesis of heart diseases such as myocardial infarction. MicroRNAs are important regulators of gene expression, which are also involved in the regulation of cardiomyocyte apoptosis. However, cardiomyocyte apoptosis regulated by microRNA (miR)-122 is largely unexplored. The aim of this study focused on the role of miR-122 in cardiomyocyte apoptosis. Cardiomyocytes were isolated from neonatal mice and primarily cultured. MiR-122 mimic and inhibitor were transfected to cardiomyocytes and verified by qRT-PCR. Cell viability and apoptosis post-transfection were assessed by MTT assay and flow cytometry, respectively. Changes in expression of caspase-8 were quantified by qRT-PCR and western blot. Results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiomyocyte; Apoptosis; MiR-122; Caspase-8; Myocardial infarction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200601

Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway
Provedor de dados: 56
Autores: Zhang,Lei-Yi; Zhou,Yue-Ying; Chen,Fei; Wang,Bing; Li,Jing; Deng,You-Wen; Liu,Wei-Dong; Wang,Zheng-Guang; Li,Ya-Wei; Li,Dong-Zhe; Lv,Guo-Hua; Yin,Bang-Liang.
Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.75% reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phosphorylation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taurine; Osteoblasts; Apoptosis; Taurine transporter; ERK.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700002

Co-culture of apoptotic breast cancer cells with immature dendritic cells: a novel approach for DC-based vaccination in breast cancer
Provedor de dados: 56
Autores: Zheng,Jin; Liu,Qiang; Yang,Jiandong; Ren,Qinyou; Cao,Wei; Yang,Jingyue; Yu,Zhaocai; Yu,Fang; Wu,Yanlan; Shi,Hengjun; Liu,Wenchao.
A dendritic cell (DC)-based vaccine strategy could reduce the risk of recurrence and improve the survival of breast cancer patients. However, while therapy-induced apoptosis of hepatocellular and colorectal carcinoma cells can enhance maturation and antigen presentation of DCs, whether this effect occurs in breast cancer is currently unknown. In the present study, we investigated the effect of doxorubicin (ADM)-induced apoptotic MCF-7 breast cancer cells on the activation of DCs. ADM-induced apoptotic MCF-7 cells could effectively induce immature DC (iDC) maturation. The mean fluorescence intensity (MFI) of DC maturity marker CD83 was 23.3 in the ADM-induced apoptotic MCF-7 cell group compared with 8.5 in the MCF-7 cell group. The MFI of DC co-stimulatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Dendritic cells; Immunotherapy; Apoptosis; Doxorubicin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600006

Effects of propofol on damage of rat intestinal epithelial cells induced by heat stress and lipopolysaccharides
Provedor de dados: 56
Autores: Tang,J.; Jiang,Y.; Tang,Y.; Chen,B.; Sun,X.; Su,L.; Liu,Z.
Gut-derived endotoxin and pathogenic bacteria have been proposed as important causative factors of morbidity and death during heat stroke. However, it is still unclear what kind of damage is induced by heat stress. In this study, the rat intestinal epithelial cell line (IEC-6) was treated with heat stress or a combination of heat stress and lipopolysaccharide (LPS). In addition, propofol, which plays an important role in anti-inflammation and organ protection, was applied to study its effects on cellular viability and apoptosis. Heat stress, LPS, or heat stress combined with LPS stimulation can all cause intestinal epithelial cell damage, including early apoptosis and subsequent necrosis. However, propofol can alleviate injuries caused by heat stress, LPS,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; Heat stress; Lipopolysaccharides; Apoptosis; Intestinal epithelial cells.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600507

Propofol inhibits proliferation, migration, and invasion but promotes apoptosis by regulation of Sox4 in endometrial cancer cells
Provedor de dados: 56
Autores: Du,Qing; Liu,Jia; Zhang,Xuezhi; Zhang,Xin; Zhu,He; Wei,Ming; Wang,Shilei.
Propofol is an intravenous sedative hypnotic agent of which the growth-inhibitory effect has been reported on various cancers. However, the roles of propofol in endometrial cancer (EC) remain unclear. This study aimed to explore the effects of propofol on EC in vitro and in vivo. Different concentrations of propofol were used to treat Ishikawa cells. Colony number, cell viability, cell cycle, apoptosis, migration, and invasion were analyzed by colony formation, MTT, flow cytometry, and Transwell assays. In addition, the pcDNA3.1-Sox4 and Sox4 siRNA plasmids were transfected into Ishikawa cells to explore the relationship between propofol and Sox4 in EC cell proliferation. Tumor weight in vivo was measured by xenograft tumor model assay. Protein levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endometrial cancer; Propofol; Cell proliferation; Apoptosis; Sox4; Wnt/β-catenin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400605

Beneficial effect of recombinant human growth hormone on the intestinal mucosa barrier of septic rats
Provedor de dados: 56
Autores: Yi,C.; Cao,Y.; Wang,S.R.; Xu,Y.Z.; Huang,H.; Cui,Y.X.; Huang,Y.
The objective of the present study was to investigate the effects of recombinant human growth hormone (rhGH) on the intestinal mucosa barrier of septic rats and explore its possible mechanism. Female Sprague-Dawley rats were randomized into three groups: control, Escherichia coli-induced sepsis (S) and treatment (T) groups. Groups S and T were subdivided into subgroups 1d and 3d, respectively. Expression of liver insulin-like growth factor-1 (IGF-1) mRNA, Bcl-2 and Bax protein levels and the intestinal Bax/Bcl-2 ratio, and plasma GH and IGF-1 levels were determined. Histological examination of the intestine was performed and bacterial translocation was determined. rhGH significantly attenuated intestinal mucosal injuries and bacterial translocation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal mucosa barrier; Growth hormone; Sepsis; Insulin-like growth factor-1; Apoptosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100006

Evasion of immune responses by Trypanosoma cruzi, the etiological agent of Chagas disease
Provedor de dados: 56
Autores: DosReis,G.A.
Infection with the protozoan parasite Trypanosoma cruzi leads to Chagas disease, which affects millions of people in Latin America. Infection with T. cruzi cannot be eliminated by the immune system. A better understanding of immune evasion mechanisms is required in order to develop more effective vaccines. During the acute phase, parasites replicate extensively and release immunomodulatory molecules that delay parasite-specific responses mediated by T cells. This immune evasion allows the parasite to spread in the host. In the chronic phase, parasite evasion relies on its replication strategy of hijacking the TGF-β signaling pathway involved in inflammation and tissue regeneration. In this article, the mechanisms of immune evasion described for T. cruzi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; T cells; GPI-anchors; TGF-β; Mucin; Apoptosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200001

Effect of curcumin on the proliferation and apoptosis of hepatic stellate cells
Provedor de dados: 56
Autores: Shu,J.C.; He,Y.J.; Lv,X.; Zhao,J.R.; Zhao,J.; Shen,Y.; Ye,G.R.; Wang,L.X.
This study was designed to investigate the effect of curcumin (diferuloylmethane) on the proliferation and apoptosis of hepatic stellate cells (HSC). The cell line HSC-T6 (1.25 x 10(5) cells/mL) was incubated with curcumin and HSC proliferation was detected by a methyl thiazolyl tetrazolium colorimetric assay. HSC apoptosis was detected by flow cytometry, transmission electron microscope and agarose gel electrophoresis. HSC proliferation was significantly inhibited in a concentration-dependent manner (10.6 to 63.5%) after incubation with 20-100 μM curcumin, compared with a control group. At 20, 40, and 60 μM, after 24 h of incubation, curcumin was associated with a significant increase in the number of HSC in the G2/M phase, and a significant decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin; Hepatic stellate cells; Proliferation; Apoptosis; Hepatic fibrosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200009

AKT inhibitor suppresses hyperthermia-induced Ndrg2 phosphorylation in gastric cancer cells
Provedor de dados: 56
Autores: Tao,Yurong; Guo,Yan; Liu,Wenchao; Zhang,Jian; Li,Xia; Shen,Lan; Ru,Yi; Xue,Yan; Zheng,Jin; Liu,Xinping; Zhang,Jing; Yao,Libo.
Hyperthermia is one of the most effective adjuvant treatments for various cancers with few side effects. However, the underlying molecular mechanisms still are not known. N-myc downstream-regulated gene 2 (NDRG2), a tumor suppressor, has been shown to be involved in diverse cellular stresses including hypoxia, lipotoxicity, etc. In addition, Ndrg2 has been reported to be related to progression of gastric cancer. In the current study, our data showed that the apoptosis rate of MKN28 cells increased relatively rapidly to 13.4% by 24 h after treatment with hyperthermia (42°C for 1 h) compared to 5.1% in control cells (P &lt; 0.05). Nevertheless, there was no obvious change in the expression level of total Ndrg2 during this process. Further investigation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperthermia; Gastric cancer; Ndrg2; Apoptosis; AKT inhibitor.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400394

Kaurene diterpene induces apoptosis in U87 human malignant glioblastoma cells by suppression of anti-apoptotic signals and activation of cysteine proteases
Provedor de dados: 56
Autores: Lizarte Neto,F.S.; Tirapelli,D.P.C.; Ambrosio,S.R.; Tirapelli,C.R.; Oliveira,F.M.; Novais,P.C.; Peria,F.M.; Oliveira,H.F.; Carlotti Junior,C.G.; Tirapelli,L.F.
Gliomas are the most common and malignant primary brain tumors in humans. Studies have shown that classes of kaurene diterpene have anti-tumor activity related to their ability to induce apoptosis. We investigated the response of the human glioblastoma cell line U87 to treatment with ent-kaur-16-en-19-oic acid (kaurenoic acid, KA). We analyzed cell survival and the induction of apoptosis using flow cytometry and annexin V staining. Additionally, the expression of anti-apoptotic (c-FLIP and miR-21) and apoptotic (Fas, caspase-3 and caspase-8) genes was analyzed by relative quantification (real-time PCR) of mRNA levels in U87 cells that were either untreated or treated with KA (30, 50, or 70 µM) for 24, 48, and 72 h. U87 cells treated with KA demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kaurenoic acid; Glioblastoma; MiR-21; C-FLIP; Apoptosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100071

Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway
Provedor de dados: 56
Autores: Wang,J.; Lu,M.L.; Dai,H.L.; Zhang,S.P.; Wang,H.X.; Wei,N.
This study investigated the in vitro and in vivo antiproliferative activity of esculetin against hepatocellular carcinoma, and clarified its potential molecular mechanisms. Cell viability was determined by the MTT (tetrazolium) colorimetric assay. In vivo antitumor activity of esculetin was evaluated in a hepatocellular carcinoma mouse model. Seventy-five C57BL/6J mice were implanted with Hepa1-6 cells and randomized into five groups (n=15 each) given daily intraperitoneal injections of vehicle (physiological saline), esculetin (200, 400, or 700 mg·kg-1·day-1), or 5-Fu (200 mg·kg-1·day-1) for 15 days. Esculetin significantly decreased tumor growth in mice bearing Hepa1-6 cells. Tumor weight was decreased by 20.33, 40.37, and 55.42% with increasing doses of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esculetin; Apoptosis; Hepatocellular carcinoma; Mitochondrial-dependent pathway; Antitumor activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300245

MFG-E8, a clearance glycoprotein of apoptotic cells, as a new marker of disease severity in chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Zhang,S.; Xie,J.G.; Su,B.T.; Li,J.L.; Hu,N.; Chen,J.; Luo,G.W.; Cui,T.P.
Milk fat globule epidermal growth factor 8 (MFG-E8) is an opsonin involved in the phagocytosis of apoptotic cells. In patients with chronic obstructive pulmonary disease (COPD), apoptotic cell clearance is defective. However, whether aberrant MFG-E8 expression is involved in this defect is unknown. In this study, we examined the expression of MFG-E8 in COPD patients. MFG-E8, interleukin (IL)-1β and transforming growth factor (TGF)-β levels were measured in the plasma of 96 COPD patients (93 males, 3 females; age range: 62.12±10.39) and 87 age-matched healthy controls (85 males, 2 females; age range: 64.81±10.11 years) using an enzyme-linked immunosorbent assay. Compared with controls, COPD patients had a significantly lower plasma MFG-E8 levels (P&lt;0.01)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; COPD; MFG-E8; Phagocytosis; Smoking.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101032

Fludarabine induces apoptosis in chronic lymphocytic leukemia - the role of P53, Bcl-2, Bax, Mcl-1, and Bag-1 proteins
Provedor de dados: 56
Autores: Faria,J.R.; Yamamoto,M.; Faria,R.M.D.; Kerbauy,J.; Oliveira,J.S.R.
The expression of P53, Bcl-2, Bax, Bag-1, and Mcl-1 proteins in CD5/CD20-positive B-chronic lymphocytic leukemia (B-CLL) cells from 30 typical CLL patients was evaluated before and after 48 h of incubation with 10-6 M fludarabine using multiparametric flow cytometric analysis. Protein expression was correlated with annexin V expression, Rai modified clinical staging, lymphocyte doubling time, and previous treatment. Our main goal was to determine the predictive value of these proteins in CLL cells in terms of disease evolution. Bcl-2 expression decreased from a median fluorescence index (MFI) of 331.71 ± 42.2 to 245.81 ± 52.2 (P &lt; 0.001) after fludarabine treatment, but there was no difference between viable cells (331.57 ± 44.6 MFI) and apoptotic cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic lymphocytic leukemia; Apoptosis; Fludarabine; P53; Bcl-2; Bax; Mcl-1; Bag-1.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300003

Immunoexpression of CD95 in chronic gastritis and gastric mucosa-associated lymphomas
Provedor de dados: 56
Autores: Vassallo,J.; Godoy Jr.,C.E.; Godoy,C.E.; Chagas,C.A.; Metze,K.; Trevisan,M.A.S.
CD95 (Fas/APO-1)-mediated apoptosis plays an important role in immunological regulation and is related to the pathogenesis of autoimmune diseases. Immunoexpression of CD95 has been reported to frequently occur in low grade non-Hodgkin lymphomas, especially of post-germinal center histogenesis, among which those originating in mucosa-associated lymphoid tissue (MALT lymphomas). However, there is no report comparing in situ immunoexpression of this marker in lymphomas and the hyperplastic lymphoid reaction (chronic gastritis) related to Helicobacter pylori infection. The purpose of the present research was to compare the intensity of lymphoid CD95 immunoexpression in 15 cases of H. pylori-related chronic gastritis and 15 gastric MALT lymphomas. CD95...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD95; FAS/APO1; Apoptosis; Chronic gastritis; MALT lymphoma.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900015

Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Provedor de dados: 56
Autores: Nunes,V.A.; Gozzo,A.J.; Juliano,M.A.; Cerqueira César,M.; Sampaio,M.U.; Sampaio,C.A.M.; Araújo,M.S.
Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Caspase activation; Apoptosis; Dietary selenium deficiency; Dietary vitamin E deficiency; Internucleosomal DNA fragmentation; Caspase substrate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010

Synergistic antitumor effect of TRAIL and adriamycin on the human breast cancer cell line MCF-7
Provedor de dados: 56
Autores: Cui,D.D.; Huang,Y.; Mao,S.H.; Chen,S.C.; Qiu,M.; Ji,L.L.; Yi,C.
The aim of the present study was to determine the effect of the combination of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and adriamycin (ADM) on the human breast cancer cell line MCF-7 and to identify potential mechanisms of apoptosis. Cell viability was analyzed by the MTT assay and the synergistic effect was assessed by the Webb coefficient. Apoptosis was quantified using the annexin V-FITC and propidium iodide staining flow cytometry. The mRNA expression of TRAIL receptors was measured by RT-PCR. Changes in the quantities of Bax and caspase-9 proteins were determined by Western blot. MCF-7 cells were relatively resistant to TRAIL (IC50 &gt;10 µg/mL), while MCF-7 cells were sensitive to ADM (IC50 &lt;10 µg/mL). A subtoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TRAIL; TRAIL receptors; Breast cancer; Adriamycin; Apoptosis; Synergism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900013

SULF2 promotes tumorigenesis and inhibits apoptosis of cervical cancer cells through the ERK/AKT signaling pathway
Provedor de dados: 56
Autores: Jiang,Tao; Chen,Zhao-Hui; Chen,Zhe; Tan,Dan.
The objective of this study was to explore the role of the SULF2-mediated ERK/AKT signaling pathway in cervical cancer. SULF2 expression was detected in tumor tissues and tumor-adjacent normal tissues from cervical cancer patients. HeLa cells were divided into six groups: control group, NC group, SULF2 siRNA group, SULF2 group, SULF2 + LY294002 group, and SULF2 + U0125 group. In each group, HeLa cells received the corresponding treatment, followed by measurement of the cellular biological characteristics and expression of the ERK/AKT signaling pathway. We also confirmed the effect of SULF2 in vivo using a xenograft model in nude mice. SULF2 was upregulated in cervical cancer tissues, which was specifically associated with the clinical stage, histological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SULF2; Cervical cancer; ERK/AKT; Proliferation; Apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200608

14-3-3 proteins in apoptosis
Provedor de dados: 56
Autores: Rosenquist,M.
The once obscure members of the 14-3-3 protein family play significant roles in the determination of cell fate. By inhibiting the pro-apoptotic BAD (Bcl-2-antagonist of cell death) and the transcription factor FKHRL-1, 14-3-3 displays important anti-apoptotic characteristics. To date, five points of interaction of 14-3-3 with the apoptotic machinery have been identified. How these interactions are regulated still remains a mystery.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 14-3-3; Apoptosis; Programmed cell death.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400001

Influence of the dopaminergic system, CREB, and transcription factor-κB on cocaine neurotoxicity
Provedor de dados: 56
Autores: Planeta,C.S.; Lepsch,L.B.; Alves,R.; Scavone,C.
Cocaine is a widely used drug and its abuse is associated with physical, psychiatric and social problems. Abnormalities in newborns have been demonstrated to be due to the toxic effects of cocaine during fetal development. The mechanism by which cocaine causes neurological damage is complex and involves interactions of the drug with several neurotransmitter systems, such as the increase of extracellular levels of dopamine and free radicals, and modulation of transcription factors. The aim of this review was to evaluate the importance of the dopaminergic system and the participation of inflammatory signaling in cocaine neurotoxicity. Our study showed that cocaine activates the transcription factors NF-κB and CREB, which regulate genes involved in cellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocaine; Apoptosis; NF-κB; CREB; BDNF; Neurotoxicity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100909

Berberine induces apoptosis via ROS generation in PANC-1 and MIA-PaCa2 pancreatic cell lines
Provedor de dados: 56
Autores: Park,S.H.; Sung,J.H.; Kim,E.J.; Chung,N.
Pancreatic cancer is the fourth leading cause of cancer death. Gemcitabine is widely used as a chemotherapeutic agent for the treatment of pancreatic cancer, but the prognosis is still poor. Berberine, an isoquinoline alkaloid extracted from a variety of natural herbs, possesses a variety of pharmacological properties including anticancer effects. In this study, we investigated the anticancer effects of berberine and compared its use with that of gemcitabine in the pancreatic cancer cell lines PANC-1 and MIA-PaCa2. Berberine inhibited cell growth in a dose-dependent manner by inducing cell cycle arrest and apoptosis. After berberine treatment, the G1 phase of PANC-1 cells increased by 10% compared to control cells, and the G1 phase of MIA-PaCa2 cells was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic cancer; Berberine; Gemcitabine; Apoptosis; Cell cycle arrest.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200111

Ischemia preconditioning is neuroprotective in a rat cerebral ischemic injury model through autophagy activation and apoptosis inhibition
Provedor de dados: 56
Autores: Xia,D.Y.; Li,W.; Qian,H.R.; Yao,S.; Liu,J.G.; Qi,X.K.
Sublethal ischemic preconditioning (IPC) is a powerful inducer of ischemic brain tolerance. However, its underlying mechanisms are still not well understood. In this study, we chose four different IPC paradigms, namely 5 min (5 min duration), 5×5 min (5 min duration, 2 episodes, 15-min interval), 5×5×5 min (5 min duration, 3 episodes, 15-min intervals), and 15 min (15 min duration), and demonstrated that three episodes of 5 min IPC activated autophagy to the greatest extent 24 h after IPC, as evidenced by Beclin expression and LC3-I/II conversion. Autophagic activation was mediated by the tuberous sclerosis type 1 (TSC1)-mTor signal pathway as IPC increased TSC1 but decreased mTor phosphorylation. Terminal deoxynucleotidyl transferase dUTP nick end...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemic preconditioning; Autophagy; Apoptosis; Focal cerebral ischemic reperfusion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700580

Two new mixed-ligand coordination polymers based on multi-N chelating ligand inhibit YAP expression and induce caspase-mediated spinal tumor cell apoptosis
Provedor de dados: 56
Autores: Sun,Yi Fu; Shao,Li Wei; Chen,Qi; Gao,Xu; Li,Fang; Wu,Chang Yan.
Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The cell viability curves showed that the two compounds have anti-proliferation activity on spinal tumor cells, and the activity of compound 1 is higher than compound 2. The annexin V-FITC/PI assay and western...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coordination polymers; Glioma cell strains; Cytotoxicity; Apoptosis; Immunoblotting.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500608

Low expression of APAF-1XL in acute myeloid leukemia may be associated with the failure of remission induction therapy
Provedor de dados: 56
Autores: Benites,B.D.; Fattori,A.; Hackel,C.; Lorand-Metze,I.; De Souza,C.A.; Schulz,E.; Costa,F.F.; Saad,S.T.O.
Apoptotic protease activating factor 1 (APAF-1) has a critical role in the regulation of apoptosis. In the present study, the mRNA expression analysis of different APAF-1 transcripts (APAF-1S, APAF-1LC, APAF-1LN, and APAF-1XL) was analyzed in bone marrow samples from 37 patients with acute myeloid leukemia (newly diagnosed, with no previous treatment). APAF-1XL and APAF-1LN transcripts (with and without an extra WD-40 repeat region, respectively) were detected in all samples, although the major form expressed was APAF-1XL in 65% of the samples (group 1), while 35% of the samples expressed primarily APAF-1LN (group 2). Only 46% of the patients presented complete remission in response to remission induction therapy (represented by less than 5% marrow blasts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: APAF-1; Acute myeloid leukemia; Apoptosis; Chemotherapy resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700004

Hydrogen sulfide postconditioning protects isolated rat hearts against ischemia and reperfusion injury mediated by the JAK2/STAT3 survival pathway
Provedor de dados: 56
Autores: Luan,Heng-Fei; Zhao,Zhi-Bin; Zhao,Qi-Hong; Zhu,Pin; Xiu,Ming-Yu; Ji,Yong.
The JAK2/STAT3 signal pathway is an important component of survivor activating factor enhancement (SAFE) pathway. The objective of the present study was to determine whether the JAK2/STAT3 signaling pathway participates in hydrogen sulfide (H2S) postconditioning, protecting isolated rat hearts from ischemic-reperfusion injury. Male Sprague-Dawley rats (230-270 g) were divided into 6 groups (N = 14 per group): time-matched perfusion (Sham) group, ischemia/reperfusion (I/R) group, NaHS postconditioning group, NaHS with AG-490 group, AG-490 (5 µM) group, and dimethyl sulfoxide (DMSO; &lt;0.2%) group. Langendorff-perfused rat hearts, with the exception of the Sham group, were subjected to 30 min of ischemia followed by 90 min of reperfusion after 20 min of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrogen sulfide; JAK2/STAT3; Apoptosis; Postconditioning; Ischemia/reperfusion injury.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000003

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells
Provedor de dados: 56
Autores: Hu,T.; Shi,J.; Jiao,X.; Zhou,J.; Yin,X.
Phosphatidylserine (PS) exposure occurs during the cell death program and fluorescein-labeled lactadherin permits the detection of PS exposure earlier than annexin V in suspended cell lines. Adherent cell lines were studied for this apoptosis-associated phenomenon to determine if PS probing methods are reliable because specific membrane damage may occur during harvesting. Apoptosis was induced in the human tongue squamous carcinoma cell line (Tca8113) and the adenoid cystic carcinoma cell line (ACC-2) by arsenic trioxide. Cells were harvested with a modified procedure and labeled with lactadherin and/or annexin V. PS exposure was localized by confocal microscopy and apoptosis was quantified by flow cytometry. The detachment procedure without trypsinization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adherent cells; Annexin V; Apoptosis; Flow cytometry; Lactadherin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900002

E3 ubiquitin ligase Cbl-b potentiates the apoptotic action of arsenic trioxide by inhibiting the PI3K/Akt pathway
Provedor de dados: 56
Autores: Yingchun,Li; Xiujuan,Qu; Jinglei,Qu; Ye,Zhang; Jing,Liu; Yuee,Teng; Xuejun,Hu; Kezuo,Hou; Yunpeng,Liu.
Arsenic trioxide (ATO) is a strong inducer of apoptosis in malignant hematological cells. Inducible phosphatidyl inositol 3 kinase (PI3K)-Akt activation promotes resistance to ATO. In the present study, we evaluated whether E3 ubiquitin ligase Cbl-b, a negative regulator of PI3K activation, is involved in the action of ATO. The effect of ATO on cell viability was measured by the Trypan blue exclusion assay or by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was determined by flow cytometry and protein expression was assayed by Western blotting. ATO decreased the viability of HL60 cells and induced cellular apoptosis, which was accompanied by transient activation of Akt. The PI3K/Akt inhibitor, LY294002,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arsenic trioxide; Apoptosis; Cbl-b; PI3K/Akt pathway.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200004

Apoptotic mimicry: an altruistic behavior in host/Leishmania interplay
Provedor de dados: 56
Autores: Wanderley,J.L.M.; Benjamin,A.; Real,F.; Bonomo,A.; Moreira,M.E.C.; Barcinski,M.A.
Apoptosis is the most common phenotype observed when cells die through programmed cell death. The morphologic and biochemical changes that characterize apoptotic cells depend on the activation of a diverse set of genes. Apoptosis is essential for multicellular organisms since their development and homeostasis are dependent on extensive cell renewal. In fact, there is strong evidence for the correlation between the emergence of multicellular organisms and apoptosis during evolution. On the other hand, no obvious advantages can be envisaged for unicellular organisms to carry the complex machinery required for programmed cell death. However, accumulating evidence shows that free-living and parasitic protozoa as well as yeasts display apoptotic markers. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Phosphatidylserine; Leishmania; Phagocyte.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600001

The regulation of apoptotic cell death
Provedor de dados: 56
Autores: Amarante-Mendes,G.P.; Green,D.R.
Apoptosis is a fundamental biological phenomenon in which the death of a cell is genetically and biochemically regulated. Different molecules are involved in the regulation of the apoptotic process. Death receptors, coupled to distinct members of the caspases as well as other adapter molecules, are involved in the initiation of the stress signals (The Indictment). Members of the Bcl-2 family control at the mitochondrial level the decision between life and death (The Judgement). The effector caspases are responsible for all morphological and biochemical changes related to apoptosis including the "eat-me" signals perceived by phagocytes and neighboring cells (The Execution). Finally, apoptosis would have little biological significance without the recognition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell death; Caspase; Mitochondria; Bcl-2; Oncogene.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900001

Tea polyphenols induce S phase arrest and apoptosis in gallbladder cancer cells
Provedor de dados: 56
Autores: Wang,Jiaqi; Pan,Yixuan; Hu,Jiacheng; Ma,Qiang; Xu,Yi; Zhang,Yijian; Zhang,Fei; Liu,Yingbin.
Gallbladder cancer (GBC) is the most common malignancy in the biliary tract. Without effective treatment, its prognosis is notoriously poor. Tea polyphenols (TPs) have many pharmacological and health benefits, including antioxidant, anti-inflammatory, anti-tumor, anti-thrombotic, antibacterial, and vasodilatory properties. However, the anti-cancer effect of TPs in human gallbladder cancer has not yet been determined. Cell viability and colony formation assay were used to investigate the cell growth. Cell cycle and apoptosis were evaluated by flow cytometry analysis. Western blot assay was used to detect the expression of proteins related to cell cycle and apoptosis. Human tumor xenografts were used to examine the effect of TPs on gallbladder cancer cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallbladder cancer; Tea polyphenols; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400614

Induction of apoptosis in cancer cells by tumor necrosis factor and butyrolactone, an inhibitor of cyclin-dependent kinases
Provedor de dados: 56
Autores: Belizário,J.E.; Sherwood,S.; Beçak,W.
Induction of apoptosis by tumor necrosis factor (TNF) is modulated by changes in the expression and activity of several cell cycle regulatory proteins. We examined the effects of TNF (1-100 ng/ml) and butyrolactone I (100 µM), a specific inhibitor of cyclin-dependent kinases (CDK) with high selectivity for CDK-1 and CDK-2, on three different cancer cell lines: WEHI, L929 and HeLa S3. Both compounds blocked cell growth, but only TNF induced the common events of apoptosis, i.e., chromatin condensation and ladder pattern of DNA fragmentation in these cell lines. The TNF-induced apoptosis events were increased in the presence of butyrolactone. In vitro phosphorylation assays for exogenous histone H1 and endogenous retinoblastoma protein (pRb) in the total cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell cycle; Cyclin-dependent kinases; Cyclin-dependent kinase chemical inhibitors; Tumor necrosis factor.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400016

Ziyuglycoside II-induced apoptosis in human gastric carcinoma BGC-823 cells by regulating Bax/Bcl-2 expression and activating caspase-3 pathway
Provedor de dados: 56
Autores: Zhu,A.K.; Zhou,H.; Xia,J.Z.; Jin,H.C.; Wang,K.; Yan,J.; Zuo,J.B.; Zhu,X.; Shan,T.
Ziyuglycoside II is an active compound of Sanguisorba officinalis L. that has anti-inflammation, antioxidation, antibiosis, and homeostasis properties. We report here on the anticancer effect of ziyuglycoside II on human gastric carcinoma BGC-823 cells. We investigated the effects of ziyuglycoside II on cell growth, cell cycle, and cell apoptosis of this cell line. Our results revealed that ziyuglycoside II could inhibit the proliferation of BGC-823 cells by inducing apoptosis but not cell cycle arrest, which was associated with regulation of Bax/Bcl-2 expression, and activation of the caspase-3 pathway. Our study is the first to report the antitumor potential of ziyuglycoside II in BGC-823 gastric cancer cells. Ziyuglycoside II may become a potential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ziyuglycoside II; BGC-823 cells; Apoptosis; Bax/Bcl-2; Caspase-3.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800670

PPARγ induces growth inhibition and apoptosis through upregulation of insulin-like growth factor-binding protein-3 in gastric cancer cells
Provedor de dados: 56
Autores: Kim,S.Y.; Kim,M.S.; Lee,M.K.; Kim,J.S.; Yi,H.K.; Nam,S.Y.; Lee,D.Y.; Hwang,P.H.
Peroxisome proliferator activator receptor-gamma (PPARγ) is a ligand-activated transcriptional factor involved in the carcinogenesis of various cancers. Insulin-like growth factor-binding protein-3 (IGFBP-3) is a tumor suppressor gene that has anti-apoptotic activity. The purpose of this study was to investigate the anticancer mechanism of PPARγ with respect to IGFBP-3. PPARγ was overexpressed in SNU-668 gastric cancer cells using an adenovirus gene transfer system. The cells in which PPARγ was overexpressed exhibited growth inhibition, induction of apoptosis, and a significant increase in IGFBP-3 expression. We investigated the underlying molecular mechanisms of PPARγ in SNU-668 cells using an IGFBP-3 promoter/luciferase reporter system. Luciferase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PPARγ; IGFBP-3; Apoptosis; Gastric cancer.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300226

Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells
Provedor de dados: 56
Autores: Zhang,L.; Liang,H.; Cao,W.; Xu,R.; Ju,X.L.
Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroblastoma; Survivin; Apoptosis; Invasion; RNA interference.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700548

The anesthetic agent sevoflurane attenuates pulmonary acute lung injury by modulating apoptotic pathways
Provedor de dados: 56
Autores: Wang,L.; Ye,Y.; Su,H.B.; Yang,J.P.
The objective of this study was to evaluate lung protection by the volatile anesthetic sevoflurane (SEVO), which inhibits apoptosis. Male Sprague-Dawley rats (250–280 g; n=18) were randomly divided into three groups. The LPS group received 5 mg/kg endotoxin (lipopolysaccharide), which induced acute lung injury (ALI). The control (CTRL) group received normal saline and the SEVO group received sevoflurane (2.5%) for 30 min after ALI was induced by 5 mg/kg LPS. Samples were collected for analysis 12 h after LPS. Lung injury was assessed by pathological observations and tissue wet to dry weight (W/D) ratios. Apoptotic index (AI) was determined by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and electron microscopy. Caspase-3 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sevofluran; Apoptosis; Pulmonary acute lung injury; Caspase-3; Bcl-2.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300702

FGFR1 is an independent prognostic factor and can be regulated by miR-497 in gastric cancer progression
Provedor de dados: 56
Autores: Xie,Gang; Ke,Qi; Ji,Yu Zu; Wang,An-qun; Jing,Meng; Zou,li-li.
Fibroblast growth factor receptor 1 (FGFR1) has been reported in gastric cancer to be a prognostic factor. However, miR-497-targeted FGFR1 has not been explored in the carcinogenesis of gastric cancer. The present study intended to revalidate the prognostic significance of FGFR1 in patients with gastric cancer, and the mechanism of miR-497-regulated FGFR1 was investigated in gastric cancer cell proliferation and apoptosis. The messenger RNA (mRNA) and protein levels were assayed by RT-qPCR and western blotting, respectively. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Cell proliferation was analyzed by CCK-8 assay. Annexin V-FITC/PI staining was used to evaluate the apoptosis in AGS and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FGFR1; MiR-497; Apoptosis; Post-transcriptional; Gastric cancer.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100602

Insulin impairs the maturation of chondrocytes in vitro
Provedor de dados: 56
Autores: Torres,E.S.; Andrade,C.V.; Fonseca,E.C.; Mello,M.A.; Duarte,M.E.L.
The precise nature of hormones and growth factors directly responsible for cartilage maturation is still largely unclear. Since longitudinal bone growth occurs through endochondral bone formation, excess or deficiency of most hormones and growth factors strongly influences final adult height. The structure and composition of the cartilaginous extracellular matrix have a critical role in regulating the behavior of growth plate chondrocytes. Therefore, the maintenance of the three-dimensional cell-matrix interaction is necessary to study the influence of individual signaling molecules on chondrogenesis, cartilage maturation and calcification. To investigate the effects of insulin on both proliferation and induction of hypertrophy in chondrocytes in vitro we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrocytes; Insulin; Micromass cultures; Apoptosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900007

Retinal protective effects of topically administered agmatine on ischemic ocular injury caused by transient occlusion of the ophthalmic artery
Provedor de dados: 56
Autores: Hong,S.; Hara,H.; Shimazawa,M.; Hyakkoku,K.; Kim,C.Y.; Seong,G.J.
Agmatine, an endogenous polyamine and putative neuromodulator, is known to have neuroprotective effects on various neurons in the central nervous system. We determined whether or not topically administered agmatine could reduce ischemic retinal injury. Transient ocular ischemia was achieved by intraluminal occlusion of the middle cerebral artery of ddY mice (30-35 g) for 2 h, which is known to also induce occlusion of the ophthalmic artery. In the agmatine group (N = 6), a 1.0 mM agmatine-containing ophthalmic solution was administered four times daily for 2 weeks before occlusion. In the control group (N = 6), a 0.1% hyaluronic acid ophthalmic solution was instilled at the same times. At 22 h after reperfusion, the eyeballs were enucleated and the retinal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Agmatine; Apoptosis; Ischemia; Neuroprotection; Retina.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300005

Fluorofenidone inhibits apoptosis of renal tubular epithelial cells in rats with renal interstitial fibrosis
Provedor de dados: 56
Autores: Yang,Hui; Zhang,Weiru; Xie,Tingting; Wang,Xuan; Ning,Wangbin.
This study aimed to investigate the mechanism of fluorofenidone (AKF-PD) in treating renal interstitial fibrosis in rats with unilateral urinary obstruction (UUO). Thirty-two male Sprague-Dawley rats were randomly divided into sham, UUO, UUO + enalapril, and UUO + AKF-PD groups. All rats, except sham, underwent left urethral obstruction surgery to establish the animal model. Rats were sacrificed 14 days after surgery, and serum was collected for renal function examination. Kidneys were collected to observe pathological changes. Immunohistochemistry was performed to assess collagen I (Col I) protein expression, and terminal deoxynucleotidyl transferase-mediated nick end-labeling staining to observe the apoptosis of renal tubular epithelial cells. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluorofenidone; Unilateral ureteral obstruction; Renal fibrosis; Apoptosis; Enalapril.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100604

Detection and analysis of apoptosis in peripheral blood cells from breast cancer patients
Provedor de dados: 56
Autores: Simão,T.A.; Andrada-Serpa,M.J.; Mendonça,G.A.S.; Marques,D.D.; Braga,M.A.; Santos,A.L.S.; Amorim,L.M.F.; Cardoso,L.E.B.; Carmo,P.A.O.; De-Moura-Gallo,C.V.
Apoptosis is a well-known specific process of cell death that normally occurs in physiological situations such as tissue or organ development and involution. During tumor growth there is a balance between proliferation and cell death which involves apoptotic mechanisms. In the present study genomic DNAs from 120 breast tumor biopsies were analyzed by agarose gel electrophoresis and none of them presented the fragmentation pattern characteristic of the apoptosis process. However, 33% of the 105 breast cancer patients clearly showed the apoptotic pattern when DNA from blood cells was analyzed. None of the DNAs from healthy volunteer blood cells showed any trace of apoptosis. Since the breast cancer patients were not receiving chemo- or hormone therapy, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Apoptosis; Breast cancer; Peripheral blood cells; Cortisol; Fas-system.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400004

Adiponectin is protective against endoplasmic reticulum stress-induced apoptosis of endothelial cells in sepsis
Provedor de dados: 56
Autores: Hou,Yun; Wang,Xi Feng; Lang,Zhi Qiang; Jin,Yin Chuan; Fu,Jia Rong; Xv,Xiao Min; Sun,Shi Tian; Xin,Xin; Zhang,Lian Shuang.
Endoplasmic reticulum (ER) stress is a critical molecular mechanism involved in the pathogenesis of sepsis. Hence, strategies for alleviating this stress may be essential for preventing cardiovascular injuries under sepsis. Adiponectin is secreted by adipocytes and its levels are decreased in sepsis. The purpose of this study was to investigate the protective effects of adiponectin treatment on endothelial cells and its mechanism. Male Wistar rats underwent cecal ligation and puncture (CLP) before being treated with adiponectin (72 and 120 μg/kg). The levels of malondialdehyde (MDA) in plasma, histological structure, and apoptosis of endothelial cells were evaluated. In vitro, human umbilical vein endothelial cells (HUVECs) were treated with adiponectin at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adiponectin; Apoptosis; Endoplasmic reticulum stress; Endothelial cell; Sepsis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200609

Noncrystalline uric acid inhibits proteoglycan and glycosaminoglycan synthesis in distal tubular epithelial cells (MDCK)
Provedor de dados: 56
Autores: Borges,F.T.; Dalboni,M.A.; Michelacci,Y.M.; Schor,N.
Hyperuricemia is associated with renal stones, not only consisting of uric acid (UrAc) but also of calcium oxalate (CaOx). Glycosaminoglycans (GAGs) are well-known inhibitors of growth and aggregation of CaOx crystals. We analyzed the effect of noncrystalline UrAc on GAG synthesis in tubular distal cells. MDCK (Madin-Darby canine kidney) cells were exposed to noncrystalline UrAc (80 µg/mL) for 24 h. GAGs were labeled metabolically and characterized by agarose gel electrophoresis. The expression of proteoglycans and cyclooxygenase 2 (COX-2) was assessed by real-time PCR. Necrosis, apoptosis and prostaglandin E2 (PGE2) were determined by acridine orange, HOESCHT 33346, and ELISA, respectively. CaOx crystal endocytosis was evaluated by flow cytometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Noncrystalline uric acid; Glycosaminoglycan; MDCK; Lipopolysaccharide; Apoptosis; Proteoglycan.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000006

Aliskiren attenuates cardiac dysfunction by modulation of the mTOR and apoptosis pathways
Provedor de dados: 56
Autores: Zhao,Zhengbo; Liu,Han; Guo,Dongmei.
Aliskiren (ALS) is well known for its antihypertensive properties. However, the potential underlying the molecular mechanism and the anti-hypertrophic effect of ALS have not yet been fully elucidated. The aim of the present study was to investigate the role of ALS in mammalian target of rapamycin (mTOR) and apoptosis signaling using in vivo and in vitro models of cardiac hypertrophy. A rat model of cardiac hypertrophy was induced by isoproterenol treatment (5 mg·kg-1·day-1) for 4 weeks, with or without ALS treatment at 20 mg·kg-1·day-1. The expression of hypertrophic, fibrotic, and apoptotic markers was determined by RT-qPCR. The protein expression of apoptotic markers mTOR and p-mTOR was assessed by western blot analysis. The proliferation of H9C2 cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aliskiren; Cardiac hypertrophy; Fibrosis; Mammalian target of rapamycin; Apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200602

Effect of iodide on Fas, Fas-ligand and Bcl-w mRNA expression in thyroid of NOD mice pretreated with methimazole
Provedor de dados: 56
Autores: Boechat,L.H.B.; Vilella,C.A.; Zollner,R.L.
Nonobese diabetic (NOD) mice and a derived strain, NOD.H.2h4, have been used as a model for experimental spontaneous thyroiditis and thyroiditis induced by iodide excess after a goiter-inducing period. Some authors have proposed that iodide, given after methimazole or propylthiouracil, is capable of inducing apoptosis in thyroid cells and that anti-thyroid drugs can modulate the expression of apoptosis components such as Fas and its ligand (Fas-L). Here we evaluated the effect of potassium iodide (20 µg/animal for 4 days, ip) given to NOD mice at the 10th week of life after exposure to methimazole (1 mg/ml) in drinking water from the 4th to the 10th week of life. Fas, Fas-L and Bcl-w expression were analyzed semiquantitatively by RT-PCR immediately after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroiditis; Nonobese diabetic mice; Apoptosis; Methimazole; Iodide; Fas mRNA; Fas-L mRNA; Bcl-w mRNA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300003

Polydatin protects H9c2 cells from hypoxia-induced injury via up-regulating long non-coding RNA DGCR5
Provedor de dados: 56
Autores: Dai,Jinhua; Ma,Jianbo; Liao,Yufeng; Luo,Xianhai; Chen,Guofang.
Polydatin (PD), a monocrystalline polyphenolic drug mainly found in the roots of Polygonum cuspidatum, has various pharmacological activities. Long non-coding RNAs (lncRNA) DiGeorge syndrome critical region gene 5 (DGCR5) was found to participate in the suppression of multiple cancers. Here, we proposed to study the effect of PD on myocardial infarction (MI) by inducing DGCR5. CCK-8 assay was performed to detect the viability of H9c2 cells. Flow cytometry was utilized to test apoptosis of H9c2 cells. These results determined the optimal concentration and effect time of hypoxia as well as PD. Si-DGCR5 was transfected into cells and the expression level was determined by qRT-PCR. Western blot was utilized to evaluate the expression of apoptosis-related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; Apoptosis; Autophagy; PI3K/AKT/mTOR pathway; MEK/ERK pathway.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200613

Early effects of estrogen on the rat ventral prostate
Provedor de dados: 56
Autores: García-Flórez,M.; Oliveira,C.A.; Carvalho,H.F.
Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Estrogen; Prostate; Stereology; Tissue kinetics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400002

Oxiracetam ameliorates cognitive deficits in vascular dementia rats by regulating the expression of neuronal apoptosis/autophagy-related genes associated with the activation of the Akt/mTOR signaling pathway
Provedor de dados: 56
Autores: Xu,Jing; Qi,Qianqian; Lv,Peiyuan; Dong,Yanhong; Jiang,Xin; Liu,Zhijuan.
Oxiracetam (ORC) is a commonly used nootropic drug for improving cognition and memory impairments. The therapeutic effect and underlying mechanism of ORC in vascular dementia (VaD) treatment remain unknown. In this study, 3-month-old male Sprague-Dawley rats with permanent bilateral common carotid artery occlusion-induced VaD were treated orally with low (100 mg/kg) or high (200 mg/kg) dose ORC once a day for 4 weeks. The results of the Morris water maze test and Nissl staining showed that ORC treatment significantly alleviated learning and memory deficits and neuronal damage in rats with VaD. Mechanistically, the protein levels of a panel of genes associated with neuronal apoptosis (Bcl-2, Bax) and autophagy (microtubule-associated protein 1 chain 3,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxiracetam; Vascular dementia; Bilateral common carotid artery occlusion; Autophagy; Apoptosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100607

Protective effect and mechanism of Lactobacillus on cerebral ischemia reperfusion injury in rats
Provedor de dados: 56
Autores: Wanchao,Shi; Chen,Ma; Zhiguo,Su; Futang,Xie; Mengmeng,Shi.
The present study was designed to investigate the protective effects and mechanism of inactivated lactobacillus (ILA) on cerebral ischemia reperfusion injury (CIRI) in rats. In this experiment, 30 male Sprague Dawley rats were randomly divided into control group, IRI groups, and ILA group. A middle cerebral artery occlusion and reperfusion model was prepared. The rats were killed after 24 hours of recovery of blood flow of cerebral ischemia resulting from 60-min occlusion. The cerebral infarction volume and neurological scores were assayed by staining and behavioral observation. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assayed by biochemical kits. Cell apoptosis was assayed by Tunnel and the Toll-like receptor (TLR)-4, IkB, and A20...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia reperfusion injury; Inactivated Lactobacillus; Apoptosis; NF-kappa B; TLR-4.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700607

Butyrate induces apoptosis in murine macrophages via caspase-3, but independent of autocrine synthesis of tumor necrosis factor and nitric oxide
Provedor de dados: 56
Autores: Ramos,M.G.; Rabelo,F.L.A.; Duarte,T.; Gazzinelli,R.T.; Alvarez-Leite,J.I.
We demonstrated that 4 mM butyrate induces apoptosis in murine peritoneal macrophages in a dose- and time-dependent manner as indicated by studies of cell viability, flow cytometric analysis of annexin-V binding, DNA ladder pattern and the determination of hypodiploid DNA content. The activity of caspase-3 was enhanced during macrophage apoptosis induced by butyrate and the caspase inhibitor z-VAD-FMK (100 µM) inhibited the butyrate effect, indicating the major role of the caspase cascade in the process. The levels of butyrate-induced apoptosis in macrophages were enhanced by co-treatment with 1 µg/ml bacterial lipopolysaccharide (LPS). However, our data indicate that apoptosis induced by butyrate and LPS involves different mechanisms. Thus, LPS-induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butyrate; Apoptosis; Caspase; Nitric oxide; Cytokine; Macrophage.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200004

Possible in vivo mechanisms involved in photodynamic therapy using tetrapyrrolic macrocycles
Provedor de dados: 56
Autores: Filip,A.G.; Clichici,S.; Daicoviciu,D.; Ion,R.M.; Tatomir,C.; Rogojan,L.; Opris,I.; Mocan,T.; Olteanu,D.; Muresan,A.
Photodynamic therapy (PDT) mediated by oxidative stress causes direct tumor cell damage as well as microvascular injury. To improve this treatment new photosensitizers are being synthesized and tested. We evaluated the effects of PDT with 5,10,15,20-tetrakis(4-methoxyphenyl)-porphyrin (TMPP) and its zinc complex (ZnTMPP) on tumor levels of malondialdehyde (MDA), reduced glutathione (GSH) and cytokines, and on the activity of caspase-3 and metalloproteases (MMP-2 and -9) and attempted to correlate them with the histological alterations of tumors in 3-month-old male Wistar rats, 180 ± 20 g, bearing Walker 256 carcinosarcoma. Rats were randomly divided into five groups: group 1, ZnTMPP+irradiation (IR) 10 mg/kg body weight; group 2, TMPP+IR 10 mg/kg body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Oxidative stress; Apoptosis; Tetrapyrrolic macrocycles; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100008

The cytotoxicity of methacryloxylethyl cetyl ammonium chloride, a cationic antibacterial monomer, is related to oxidative stress and the intrinsic mitochondrial apoptotic pathway
Provedor de dados: 56
Autores: Sai,Ma; Le-qun,Shan; Yu-hong,Xiao; Fang,Li; Li,Huang; Shen,Lijuan; Ji-hua,Chen.
Antibacterial monomers incorporated in dentin bonding systems may have toxic effects on the pulp. Thus, the cytotoxicity of antibacterial monomers and its underlying mechanisms must be elucidated to improve the safety of antibacterial monomer application. The influence of an antibacterial monomer, methacryloxylethyl cetyl ammonium chloride (DMAE-CB), on the vitality of L929 mouse fibroblasts was tested using MTT assay. Cell cycle progression was studied using flow cytometry. Production of intracellular reactive oxygen species (ROS) after DMAE-CB treatment was measured using 2,7-dichlorodihydrofluorescein diacetate staining and flow cytometry analysis. Loss of mitochondrial membrane potential, disturbance of Bcl-2 and Bax expression, as well as release of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quaternary ammonium compounds; Dental monomer; Cytotoxicity; Apoptosis; Reactive oxygen species; Methacryloxylethyl cetyl ammonium chloride.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100008

Galectin-1, an alternative signal for T cell death, is increased in activated macrophages
Provedor de dados: 56
Autores: Rabinovich,G.A.; Riera,C.M.; Sotomayor,C.E.
Galectin-1 belongs to an evolutionarily conserved family of animal ß-galactoside-binding proteins, which exert their functions by crosslinking the oligosaccharides of specific glycoconjugate ligands. During the past decade, attempts to identify the functional role of galectin-1 suggested participation in the regulation of the immune response. Only in the last few years has the molecular mechanism involved in these properties been clearly elucidated, revealing a critical role for galectin-1 as an alternative signal in the generation of T cell death. In the present study we will discuss the latest advances in galectin research in the context of the regulation of the immune response, not only at the central level but also at the periphery. Moreover, we will...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galectin-1; Apoptosis; Immunomodulation; Macrophage.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500009

Propofol inhibits burn injury-induced hyperpermeability through an apoptotic signal pathway in microvascular endothelial cells
Provedor de dados: 56
Autores: Tian,K.Y.; Liu,X.J.; Xu,J.D.; Deng,L.J.; Wang,G.
Recent studies have revealed that an intrinsic apoptotic signaling cascade is involved in vascular hyperpermeability and endothelial barrier dysfunction. Propofol (2,6-diisopropylphenol) has also been reported to inhibit apoptotic signaling by regulating mitochondrial permeability transition pore (mPTP) opening and caspase-3 activation. Here, we investigated whether propofol could alleviate burn serum-induced endothelial hyperpermeability through the inhibition of the intrinsic apoptotic signaling cascade. Rat lung microvascular endothelial cells (RLMVECs) were pretreated with propofol at various concentrations, followed by stimulation with burn serum, obtained from burn-injury rats. Monolayer permeability was determined by transendothelial electrical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Burn injury; Hyperpermeability; Propofol; Apoptosis; Endothelial cells.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500401

Apoptosis of sensory neurons and satellite cells after sciatic nerve transection in C57BL/6J mice
Provedor de dados: 56
Autores: Oliveira,A.L.R.
The rate of axonal regeneration, after sciatic nerve lesion in adult C57BL/6J mice, is reduced when compared to other isogenic strains. It was observed that such low regeneration was not due just to a delay, since neuronal death was observed. Two general mechanisms of cell death, apoptosis and necrosis, may be involved. By using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique, we demonstrated that a large number of sensory neurons, as well as satellite cells found in the dorsal root ganglia, were intensely labeled, thus indicating that apoptotic mechanisms were involved in the death process. Although almost no labeled neurons or satellite cells were observed one week after transection, a more than ten-fold increase in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: TUNEL; Apoptosis; Axotomy; Neuronal death; Satellite cells; Isogenic mice.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300012

Silencing of augmenter of liver regeneration inhibited cell proliferation and triggered apoptosis in U266 human multiple myeloma cells
Provedor de dados: 56
Autores: Zeng,H.Q.; Luo,Y.; Lou,S.F.; Liu,Q.; Zhang,L.; Deng,J.C.
Augmenter of liver regeneration (ALR) is a thermostable cytokine that was originally identified to promote the growth of hepatocytes. This study was conducted to explore the expression and function of ALR in multiple myeloma (MM), a common hematologic malignancy. Real-time PCR and western blot analysis were performed to detect the expression of ALR in U266 human MM cells and healthy peripheral blood mononuclear cells (PBMCs). U266 MM cells were exposed to 20 or 40 μg/mL of recombinant ALR and tested for cell proliferation. Small interfering RNA-mediated silencing of ALR was done to investigate the role of ALR in cell proliferation, apoptosis, and cytokine production. Compared to PBMCs, U266 MM cells exhibited significantly higher levels of ALR at both the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Augmenter of liver regeneration; Hematologic cancer; Proliferation; Small interfering RNA.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000611

Detection of early apoptosis and cell death in T CD4+ and CD8+ cells from lesions of patients with localized cutaneous leishmaniasis
Provedor de dados: 56
Autores: Bertho,A.L.; Santiago,M.A.; Da-Cruz,A.M.; Coutinho,S.G.
Human localized cutaneous leishmaniasis (LCL), induced by Leishmania braziliensis, ranges from a clinically mild, self-healing disease with localized cutaneous lesions to severe forms which can present secondary metastatic lesions. The T cell-mediated immune response is extremely important to define the outcome of the disease; however, the underlying mechanisms involved are not fully understood. A flow cytometric analysis of incorporation of 7-amino actinomycin D and CD4+ or CD8+ T cell surface phenotyping was used to determine whether different frequencies of early apoptosis or accidental cell death occur at different stages of LCL lesions. When all cells obtained from a biopsy sample were analyzed, larger numbers of early apoptotic and dead cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Flow cytometry; Human leishmaniasis; Cell death; T cells.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300010

Polydatin inhibits cell proliferation, invasion and migration, and induces cell apoptosis in hepatocellular carcinoma
Provedor de dados: 56
Autores: Jiao,Yang; Wu,Yan; Du,Dong.
Polydatin, a small molecule from Polygonum cuspidatum, has many biological functions, particularly anti-cancer effects. However, the anti-cancer effects of polydatin in hepatocellular carcinoma (HCC) have not been examined yet. In the present study, MTT assay, BrdU assay, transwell invasion assay, and wound healing assay were performed to determine cell proliferation, invasion and migration. Flow cytometry and TUNEL assay were used to measure cell apoptosis. Quantitative real-time PCR and western blotting assays were used to determine mRNA and protein expression levels. Xenograft experiment was performed to determine the in vivo anti-tumor effect of polydatin. Immunostaining was performed to analyze the expression of caspase-3 and Ki-67. Our results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polydatin; Hepatocellular carcinoma; Proliferation; Apoptosis; Wnt/beta-catenin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400608

Primary mechanism of apoptosis induction in a leukemia cell line by fraction FA-2-b-ß prepared from the mushroom Agaricus blazei Murill
Provedor de dados: 56
Autores: Gao,L.; Sun,Y.; Chen,C.; Xi,Y.; Wang,J.; Wang,Z.
Agaricus blazei Murill is a native Brazilian mushroom which functions primarily as an anticancer substance in transplanted mouse tumors. However, the mechanism underlying this function of A. blazei Murill remains obscure. The present study was carried out to investigate the effect of fraction FA-2-b-ß, an RNA-protein complex isolated from A. blazei Murill, on human leukemia HL-60 cells in vitro. Typical apoptotic characteristics were determined by morphological methods using DNA agarose gel electrophoresis and flow cytometry. The growth suppressive effect of fraction FA-2-b-ß on HL-60 cells in vitro occurred in a dose- (5-80 µg/mL) and time-dependent (24-96 h) manner. The proliferation of HL-60 cells (1 x 10(5) cells/mL) treated with 40 µg/mL of fraction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei Murill; Fraction FA-2-b-ß; Apoptosis; Caspase-3; Telomerase; HL-60 cells.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100015

Glycyrrhizin attenuates endotoxin- induced acute liver injury after partial hepatectomy in rats
Provedor de dados: 56
Autores: Tang,B.; Qiao,H.; Meng,F.; Sun,X.
Massive hepatectomy associated with infection induces liver dysfunction, or even multiple organ failure and death. Glycyrrhizin has been shown to exhibit anti-oxidant and anti-inflammatory activities. The aim of the present study was to investigate whether glycyrrhizin could attenuate endotoxin-induced acute liver injury after partial hepatectomy. Male Wistar rats (6 to 8 weeks old, weighing 200-250 g) were randomly assigned to three groups of 24 rats each: sham, saline and glycyrrhizin. Rats were injected intravenously with lipopolysaccharide (LPS) 24 h after 70% hepatectomy. Glycyrrhizin, pre-administered three times with 24 h intervals 48 h before hepatectomy, prolonged the survival of rats submitted to partial hepatectomy and LPS injection, compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycyrrhizin; Lipopolysaccharide; Hepatectomy; Inflammatory cytokine; Apoptosis; Endotoxemia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200007

Antioxidant effect of mogrosides against oxidative stress induced by palmitic acid in mouse insulinoma NIT-1 cells
Provedor de dados: 56
Autores: Xu,Q.; Chen,S.Y.; Deng,L.D.; Feng,L.P.; Huang,L.Z.; Yu,R.R.
Excessive oxidative stress in pancreatic β cells, caused by glucose and fatty acids, is associated with the pathogenesis of type 2 diabetes. Mogrosides have shown antioxidant and antidiabetic activities in animal models of diabetes, but the underlying mechanisms remain unclear. This study evaluated the antioxidant effect of mogrosides on insulinoma cells under oxidative stress caused by palmitic acid, and investigated the underlying molecular mechanisms. Mouse insulinoma NIT-1 cells were cultured in medium containing 0.75 mM palmitic acid, mimicking oxidative stress. The effects of 1 mM mogrosides were determined with the dichlorodihydrofluorescein diacetate assay for intracellular reactive oxygen species (ROS) and FITC-Annexin V/PI assay for cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mogrosides; Insulin-secreting cells; Oxidative stress damage; Apoptosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100949

Mitochondria, calcium and pro-apoptotic proteins as mediators in cell death signaling
Provedor de dados: 56
Autores: Smaili,S.S.; Hsu,Y.-T.; Carvalho,A.C.P.; Rosenstock,T.R.; Sharpe,J.C.; Youle,R.J.
Cellular Ca2+ signals are crucial in the control of most physiological processes, cell injury and programmed cell death through the regulation of a number of Ca2+-dependent enzymes such as phospholipases, proteases, and nucleases. Mitochondria along with the endoplasmic reticulum play pivotal roles in regulating intracellular Ca2+ content. Mitochondria are endowed with multiple Ca2+ transport mechanisms by which they take up and release Ca2+ across their inner membrane. During cellular Ca2+ overload, mitochondria take up cytosolic Ca2+, which in turn induces opening of permeability transition pores and disrupts the mitochondrial membrane potential (&lt;FONT FACE=Symbol&gt;Dy&lt;/FONT&gt;m). The collapse of &lt;FONT FACE=Symbol&gt;Dy&lt;/FONT&gt;m along...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+; Mitochondrial Ca2+ uptake; Mitochondrial Ca2+ efflux; Permeability transition; Apoptosis; Bcl-2 family; Bax and apoptosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200004

Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro
Provedor de dados: 56
Autores: Yang,H.; Zhu,Y.T.; Cheng,R.; Shao,M.Y.; Fu,Z.S.; Cheng,L.; Wang,F.M.; Hu,T.
Lipopolysaccharide exerts many effects on many cell lines, including cytokine secretion, and cell apoptosis and necrosis. We investigated the in vitro effects of lipopolysaccharide on apoptosis of cultured human dental pulp cells and the expression of Bcl-2 and Bax. Dental pulp cells showed morphologies typical of apoptosis after exposure to lipopolysaccharide. Flow cytometry showed that the rate of apoptosis of human dental pulp cells increased with increasing lipopolysaccharide concentration. Compared with controls, lipopolysaccharide promoted pulp cell apoptosis (P &lt; 0.05) from 0.1 to 100 μg/mL but not at 0.01 μg/mL. Cell apoptosis was statistically higher after exposure to lipopolysaccharide for 3 days compared with 1 day, but no difference was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental pulp cells; Lipopolysaccharide; Apoptosis; Bax; Bcl-2.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100003

Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway
Provedor de dados: 56
Autores: Li,Yiwen; Xu,Yuqing; Lei,Bo; Wang,Wenxiu; Ge,Xin; Li,Jingrui.
Rhein is a primary anthraquinone found in the roots of a traditional Chinese herb, rhubarb, and has been shown to have some anticancer effects. The aim of the present study was to investigate the effect of rhein on the apoptosis of the human gastric cancer line SGC-7901 and to identify the mechanism involved. SGC-7901 cells were cultured and treated with rhein (0, 50, 100, 150, and 200 µM) for 24, 48, or 72 h. Relative cell viability assessed by the MTT assay after treatment was 100, 99, 85, 79, 63% for 24 h; 100, 98, 80, 51, 37% for 48 h, and 100, 97, 60, 36, 15% for 72 h, respectively. Cell apoptosis was detected with TUNEL staining and quantified with flow cytometry using annexin FITC-PI staining at 48 h after 100, 200 and 300 µm rhein. The percentage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhein; SGC-7901 cells; MTT; TUNEL; Flow cytometry; Apoptosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100009

Curcumin induces human HT-29 colon adenocarcinoma cell apoptosis by activating p53 and regulating apoptosis-related protein expression
Provedor de dados: 56
Autores: Song,G.; Mao,Y.B.; Cai,Q.F.; Yao,L.M.; Ouyang,G.L.; Bao,S.D.
Curcumin, a major yellow pigment and active component of turmeric, has multiple anti-cancer properties. However, its molecular targets and mechanisms of action on human colon adenocarcinoma cells are unknown. In the present study, we examined the effects of curcumin on the proliferation of human colon adenocarcinoma HT-29 cells by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide method and confirmed the curcumin-induced apoptosis by morphology and DNA ladder formation. At the same time, p53, phospho-p53 (Ser15), and other apoptosis-related proteins such as Bax, Bcl-2, Bcl-xL, pro-caspase-3, and pro-caspase-9 were determined by Western blot analysis. The colon adenocarcinoma cells were treated with curcumin (0-75 µM) for 0-24 h. We observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin; Apoptosis; HT-29 cells; P53; Phosphorylation; DNA ladder.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200007

Apoptosis in parasites and parasite-induced apoptosis in the host immune system: a new approach to parasitic diseases
Provedor de dados: 56
Autores: Barcinski,M.A.; DosReis,G.A.
Apoptosis, a form of programmed cell death (PCD), has been described as essential for normal organogenesis and tissue development, as well as for the proper function of cell-renewal systems in adult organisms. Apoptosis is also pivotal in the pathogenesis of several different diseases. In this paper we discuss, from two different points of view, the role of apoptosis in parasitic diseases. The description of apoptotic death in three different species of heteroxenic trypanosomatids is reviewed, and considerations on the phylogenesis of apoptosis and on the eventual role of PCD on their mechanism of pathogenesis are made. From a different perspective, an increasing body of evidence is making clear that regulation of host cell apoptosis is an important factor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Parasites; Host cells; Host immune system; Programmed cell death; Trypanosomatids.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400003

miR-378a-5p and miR-630 induce lens epithelial cell apoptosis in cataract via suppression of E2F3
Provedor de dados: 56
Autores: Gao,Weiwei; Zhou,Xiaoqing; Lin,Ruihua.
Cataract, an eye disease that threatens the health of millions of people, brings about severe economic burden for patients and society. MicroRNA (miR)-378a-5p and miR-630 were recognized as essential regulators in multiple cancers. However, the exact functions of miR-378a-5p and miR-630 in cataract are still unclear. The expression of miR-378a-5p, miR-630, and E2F transcription factor 3 (E2F3) in tissues and cells was measured by quantitative real-time polymerase chain reaction. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was used to evaluate cell viability. Flow cytometry was conducted to analyze cell apoptosis. The interaction between E2F3 and miR-378a-5p or miR-630 was confirmed by dual-luciferase reporter assay. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-378a-5p; MiR-630; E2F3; Apoptosis; Cataract.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500609

miR-26a-5p protects against myocardial ischemia/reperfusion injury by regulating the PTEN/PI3K/AKT signaling pathway
Provedor de dados: 56
Autores: Xing,Xiaowei; Guo,Shuang; Zhang,Guanghao; Liu,Yusheng; Bi,Shaojie; Wang,Xin; Lu,Qinghua.
Reperfusion strategies in acute myocardial infarction (AMI) can cause a series of additional clinical damage, defined as myocardial ischemia/reperfusion (I/R) injury, and thus there is a need for effective therapeutic methods to attenuate I/R injury. miR-26a-5p has been proven to be an essential regulator for biological processes in different cell types. Nevertheless, the role of miR-26a-5p in myocardial I/R injury has not yet been reported. We established an I/R injury model in vitro and in vivo. In vitro, we used cardiomyocytes to simulate I/R injury using hypoxia/reoxygenation (H/R) assay. In vivo, we used C57BL/6 mice to construct I/R injury model. The infarct area was examined by TTC staining. The level of miR-26a-5p and PTEN was determined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myocardial infarction; MiR-26a-5p; PTEN; Apoptosis; PI3K/AKT pathway; Myocardial ischemia/reperfusion injury.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200601

Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells
Provedor de dados: 56
Autores: Ji,C.; Ren,F.; Xu,M.
Nasopharyngeal carcinoma is a common malignancy in Southern China of uncertain etiologic origin. Diallyl trisulfide (DATS), one of the major components of garlic (Allium sativum), is highly bactericidal and fungicidal. In this study, we investigated the function of p38 mitogen-activated protein kinase (MAPK) and caspase-8 in DATS-induced apoptosis of human CNE2 cells using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], flow cytometry assay, and Western blotting. After CNE2 cells were treated with DATS (50, 100, or 150 μM) for 24 h, cell viability rates were 75.9, 63.4 and 39.6%, and apoptosis rates were 24.5, 36.9, and 62.4%, respectively. The data showed that DATS induced CNE2 cell death in a dose-dependent manner. After human CNE2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DATS; P38 MAPK; Caspase-8; Inhibitor; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900003

Evaluation of chromosomal abnormalities by cIg-FISH and association with proliferative and apoptotic indexes in multiple myeloma
Provedor de dados: 56
Autores: Linardi,C.C.G.; Martinez,G.; Velloso,E.D.R.P.; Leal,A.M.; Kumeda,C.A.; Buccheri,V.; Azevedo,R.S.; Peliçario,L.M.; Dorlhiac-Llacer,P.
Eighty-six newly diagnosed multiple myeloma (MM) patients from a public hospital of São Paulo (Brazil) were evaluated by cIg-FISH for the presence of del(13)(q14), t(4;14)(p16.3;q32) and del(17)(p13). These abnormalities were observed in 46.5, 9.3, and 7.0% of the patients, respectively. In order to identify the possible role of del(13)(q14) in the physiopathology of MM, we investigated the association between this abnormality and the proliferative and apoptotic indexes of plasma cells. When cases demonstrating t(4;14)(p16.3;q32) and del(17)(p13) were excluded from the analysis, we observed a trend towards a positive correlation between the proportion of cells carrying del(13)(q14) and plasma cell proliferation, determined by Ki-67 expression (r = 0.23, P...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiple myeloma; Cell proliferation; Apoptosis; Prognosis; Fluorescence in situ hybridization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100012

Lentiviral-mediated RNAi targeting caspase-3 inhibits apoptosis induced by serum deprivation in rat endplate chondrocytes in vitro
Provedor de dados: 56
Autores: Ding,L.; Wu,J.P.; Xu,G.; Zhu,B.; Zeng,Q.M.; Li,D.F.; Lu,W.
Current studies find that degenerated cartilage endplates (CEP) of vertebrae, with fewer diffusion areas, decrease nutrient supply and accelerate intervertebral disc degeneration. Many more apoptotic cells have been identified in degenerated than in normal endplates, and may be responsible for the degenerated grade. Previous findings suggest that inhibition of apoptosis is one possible approach to improve disc regeneration. It is postulated that inhibition of CEP cell apoptosis may be responsible for the regeneration of endplates. Caspase-3, involved in the execution phase of apoptosis, is a candidate for regulating the apoptotic process. In the present study, CEP cells were incubated in 1% fetal bovine serum. Activated caspases were detected to identify...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cartilaginous endplate; Chondrocytes; RNA interference; Apoptosis; Caspase-3; Serum deprivation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600445

Ghrelin attenuates the growth of HO-8910 ovarian cancer cells through the ERK pathway
Provedor de dados: 56
Autores: Bai,R.X.; Wang,W.P.; Zhao,P.W.; Li,C.B.
Ovarian cancer is one of the most common causes of death from gynecologic tumors and is an important public health issue. Ghrelin is a recently discovered bioactive peptide that acts as a natural endogenous ligand of the growth hormone secretagogue receptor (GHSR). Several studies have identified the protective effects of ghrelin on the mammalian reproductive system. However, little research has been done on the effects of ghrelin on ovarian cancer cells, and the underlying mechanisms of these effects. We sought to understand the potential involvement of mitogen-activated protein kinases (MAPKs) in ghrelin-mediated inhibition of growth of the ovarian line HO-8910. We applied different concentrations of ghrelin and an inhibitor of the ghrelin receptor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ghrelin; MAPKs; HO-8910; Apoptosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300602

Advanced glycation end products (AGEs) and their receptor (RAGE) induce apoptosis of periodontal ligament fibroblasts
Provedor de dados: 56
Autores: Li,D.X.; Deng,T.Z.; Lv,J.; Ke,J.
Diabetics have an increased prevalence of periodontitis, and diabetes is one of the causative factors of severe periodontitis. Apoptosis is thought to be involved in this pathogenic relationship. The aim of this study was to investigate apoptosis in human periodontal ligament (PDL) fibroblasts induced by advanced glycation end products (AGEs) and their receptor (RAGE). We examined the roles of apoptosis, AGEs, and RAGE during periodontitis in diabetes mellitus using cultured PDL fibroblasts that were treated by AGE-modified bovine serum albumin (AGE-BSA), bovine serum albumin (BSA) alone, or given no treatment (control). Microscopy and real-time quantitative PCR indicated that PDL fibroblasts treated with AGE-BSA were deformed and expressed higher levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAGE; Apoptosis; Diabetes mellitus; Periodontal diseases.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201036

Gentamicin-induced preconditioning of proximal tubular LLC-PK1 cells stimulates nitric oxide production but not the synthesis of heat shock protein
Provedor de dados: 56
Autores: Pessoa,E.A.; Convento,M.B.; Silva,R.G.; Oliveira,A.S.; Borges,F.T.; Schor,N.
Nephrotoxicity is the main side effect of antibiotics such as gentamicin. Preconditioning has been reported to protect against injuries as ischemia/reperfusion. The objective of the present study was to determine the effect of preconditioning with gentamicin on LLC-PK1 cells. Preconditioning was induced in LLC-PK1 cells by 24-h exposure to 2.0 mM gentamicin (G/IU). After 4 or 15 days of preconditioning, cells were again exposed to gentamicin (2.0 mM) and compared to untreated control or G/IU cells. Necrosis and apoptosis were assessed by acridine orange and HOESCHT 33346. Nitric oxide (NO) and endothelin-1 were assessed by the Griess method and available kit. Heat shock proteins were analyzed by Western blotting. After 15 days of preconditioning, LLC-PK1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pre-conditioning; Gentamicin; Nitric oxide; LLC-PK1 cells; Acute kidney injury; Apoptosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700005

Paullinia cupana Mart var. sorbilis, guaraná, reduces cell proliferation and increases apoptosis of B16/F10 melanoma lung metastases in mice
Provedor de dados: 56
Autores: Fukumasu,H.; Avanzo,J.L.; Nagamine,M.K.; Barbuto,J.A.; Rao,K.V.; Dagli,M.L.Z.
We showed that guaraná (Paullinia cupana Mart var. sorbilis) had a chemopreventive effect on mouse hepatocarcinogenesis and reduced diethylnitrosamine-induced DNA damage. In the present experiment, we evaluated the effects of guaraná in an experimental metastasis model. Cultured B16/F10 melanoma cells (5 x 10(5) cells/animal) were injected into the tail vein of mice on the 7th day of guaraná treatment (2.0 mg P. cupana/g body weight, per gavage) and the animals were treated with guaraná daily up to 14 days until euthanasia (total treatment time: 21 days). Lung sections were obtained for morphometric analysis, apoptotic bodies were counted to calculate the apoptotic index and proliferating cell nuclear antigen-positive cells were counted to determine the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Melanoma; Guaraná; Metastases; Apoptosis; Growth control.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400008

Mechanism of bactericidal activity of Silver Nitrate - a concentration dependent bi-functional molecule
Provedor de dados: 58
Autores: Pandian,Sureshbabu Ram Kumar; Deepak,Venkataraman; Kalishwaralal,Kalimuthu; Viswanathan,Pushpa; Gurunathan,Sangiliyandi.
Silver nitrate imparts different functions on bacteria depending upon its concentration. At lower concentration it induced synthesis of nanoparticles, whereas at higher concentrations it induced cell death. Bacillus licheniformis was used as model system. The MIC was 5 mM, and it induced catalase production, apoptotic body formation and DNA fragmentation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver nitrate; Bacillus licheniformis; Nanoparticle synthesis; Apoptosis; DNA fragmentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300033

Cytotoxic and apoptotic potential of Myristica fragrans Houtt. (mace) extract on human oral epidermal carcinoma KB cell lines
Provedor de dados: 60
Autores: Rengasamy,Gayathri; Venkataraman,Anuradha; Veeraraghavan,Vishnu Priya; Jainu,Mallika.
Several studies have revealed that certain naturally occurring medicinal plants inhibit the growth of various cancers. The present study was conducted to evaluate cytotoxicity and apoptotic induction potential of Myristica fragrans Houtt mace extract. The cytotoxic activity of the Myristica fragrans Houtt mace acetone extract was assayed by MTT assay on human oral epidermal carcinoma KB cell lines. KB cells were incubated with different concentration of mace extract ranging from 25 to 125 μg/mL for 24hrs. The apoptotic induction potential was also studied by the analysis of Bcl-2 protein and gene expression in mace extract incubated KB cell lines using western blotting technique and real-time polymerase chain reaction. The mace extract exhibited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myristica fragrans Houtt; (mace)/extract; Apoptosis; Cytotoxicity; KB cell lines; MTT assay; Bcl-2 gene.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300629

Essential oil from Euphorbia esula inhibits proliferation and induces apoptosis in HepG2 cells via mitochondrial dysfunction
Provedor de dados: 60
Autores: Lv,Dan; Pan,Li-hong; Zhang,Ren; Yang,Jie; Chen,Hao; Wen,Yanzhang; Huang,Mi; Ma,Xinhua; Wang,Qiang; Yang,Xinzhou.
Hepatocellular carcinoma is one of the most prevalent malignancies and a leading cause of cancer-related mortality worldwide. However, the therapies to prevent hepatocellular carcinoma are still limited and the emergence of drug resistance leads to the development of new anti-cancer drugs and combinational chemotherapy regimens. Our study was aimed to explore the anticancer effects of the essential oil extract (EEEO) from Euphorbia esula which has been widely used in traditional Chinese folk medicine and possessed potential cytotoxic effects in several human tumor cells. However, the mechanisms of EEEO-induced anti-proliferation and apoptosis have not been completely elucidated. In this study, EEEO was prepared by hydro-distillation and the main chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbia esula; Hepatocellular carcinoma; Apoptosis; Reactive oxygen species; Mitochondrial pathway.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100506

Passiflora mucronata leaves extracts obtained from different methodologies: a phytochemical study based on cytotoxic and apoptosis activities of triterpenes and phytosterols constituents
Provedor de dados: 60
Autores: Silva,Isabel Cristina Vieira da; Oliveira,Pollyana Felix de; Barbosa,Gleyce Moreno; Wessjohann,Ludger A.; Cardozo-Filho,Lucio; Holandino,Carla; Muzitano,Michelle Frazão; Leal,Ivana Correa Ramos.
Cancer is one of the most prevalent diseases worldwide and the natural products could be a source of bioactive compounds. Passiflora mucronata (PM) belongs to a very known vegetal genus, although, there are no studies about cytotoxic activity or isolated compounds. Different extracts from PM were obtained by liquid-liquid partition (P), Soxhlet (Sox) and supercritical fluid (SFE1-5) extraction techniques, being compared concerning their yields, chemical profile and cytotoxicity. The Sox extracts showed the highest yields (6.03%: hexane; 2.51%: dichloromethane) followed by SFE (from 4.34 to 1.63%) and partitions (1.06 and 2.26%). The hexane partition (HP) showed the best cytotoxic activity against K562 cell line (IC50 = 18.72 µg.mL-1). From HP, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cytotoxic; Passiflora mucronata; Stigmasterol; Triterpenes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100510

Deregulated expression of A1, Bcl-2, Bcl-xL, and Mcl-1 antiapoptotic proteins and Bid, Bad, and Bax proapoptotic genes in polycythemia vera patients
Provedor de dados: 60
Autores: Gasparotto,Elainy Patricia Lino; Tognon,Raquel; Ferreira,Aline Fernanda; Oliveira,Gislane Lelis Vilela; Palma,Patrícia Vianna Bonini; Zanichelli,Maria Aparecida; Souto,Elizabeth Xisto; Velano,Carlos Eduardo Engel; Simões,Belinda Pinto; Carrara,Rita de Cassia Viu; Kashima,Simone; Covas,Dimas Tadeu; Castro,Fabíola Attie de; Souza,Ana Maria de.
Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycythemia vera; Gene mutation; Gene expression; Apoptosis; Bcl-2 family members.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400025

Hepatic protective effect of grape seed proanthocyanidin extract against Gleevec-induced apoptosis, liver Injury and Ki67 alterations in rats
Provedor de dados: 60
Autores: Al-Rasheed,Nawal Mohammed; El-Masry,Thanaa Ahmed; Tousson,Ehab; Hassan,Hanan Mohammad; Al-Ghadeer,Areej.
ABSTRACT Gleevec (imatinib) is an antineoplastic chemotherapeutic agent used in the treatment of many types of cancer. The current study was conducted to examine the possible modifying effects of grape seeds proanthocyandins extract (GSPE) against apoptosis, liver injury and Ki67 alterations induced by Gleevec in male rats. 40 male albino rats were equally divided into four groups (First and second groups were control and GSPE groups; third group was Gleevec group and fourth group was treated with Gleevec and GSPE). Gleevec induced elevations in P53 and depletion of Bcl2 levels in liver tissues were compared with the control group. Liver sections in rats treated with Gleevec exhibited marked cellular infiltrations, vacuolar degeneration hepatocytes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemotherapy; Gleevec/effects; Gleevec/Imatinib; Proanthocyandins; Liver; Apoptosis; Proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200612