Consulta: creatorFacets:"Quillien, Virgile"
Registros recuperados: 26
Data/hora: 11/06/2024 10:11:17

Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)
Provedor de dados: 5
Autores: Fuhrmann, Marine; Petton, Bruno; Quillien, Virgile; Faury, Nicole; Morga, Benjamin; Pernet, Fabrice.
Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the...
Tipo: Text
Palavras-chave: Bivalve; Herpesviridae; Infection; Disease transmission; Risk analysis.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00350/46100/45781.pdf

In vivo RNA interference in oyster -vasa silencing inhibits germ cell development
Provedor de dados: 5
Autores: Fabioux, Caroline; Corporeau, Charlotte; Quillien, Virgile; Favrel, Pascal; Huvet, Arnaud.
This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas,...
Tipo: Text
Palavras-chave: Vasa; RNAi; Marine bivalve; Germline; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6641.pdf

Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana; Milan, Massimo; Guo, Xinmei; Li, Li; Zhang, Guofan; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.
AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism...
Tipo: Text
Palavras-chave: Marine bivalve; Crassostrea gigas; Hypoxia; AMP-activated protein kinase; Alternative splicing; Adductor muscle.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00119/22998/21397.pdf

In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Fleury, Elodie; Corporeau, Charlotte; Quillien, Virgile; Daniel, Jean-yves; Riviere, Guillaume; Boudry, Pierre; Fabioux, Caroline.
We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated...
Tipo: Text
Palavras-chave: Marine bivalve; Crassostrea gigas; Reproduction; RNA interference; Transforming growth factor-beta.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19794/17486.pdf

Understanding the mechanisms involved in the high sensitivity of Pecten maximus larvae to aeration
Provedor de dados: 5
Autores: Pauletto, Marianna; Di Camillo, Barbara; Miner, Philippe; Huvet, Arnaud; Quillien, Virgile; Milan, Massimo; Ferraresso, Serena; Pegolo, Sara; Patarnello, Tomaso; Bargelloni, Luca.
Among reared bivalves, some “novel species”, such as the great scallop, Pecten maximus, have experienced more difficulty with routine reproduction due to their high sensitivity to biological, chemical, and physical stress during stages of early development. Working with high larval densities requires the use of aeration systems to provide optimal larval suspension and feed distribution. The high susceptibility of the great scallop to aeration in small-volume systems may impose an important limitation in hatchery-based practices. The present study aimed to investigate the processes impacted by aeration in P. maximus veliger larvae exposed to continuous aeration in small-volume tanks (10 L). Aeration appeared as major stressor that was responsible for early...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00453/56420/58327.pdf

Bioactive extracellular compounds produced by the dinoflagellate Alexandrium minutum are highly detrimental for oysters
Provedor de dados: 5
Autores: Castrec, Justine; Soudant, P.; Payton, L.; Tran, D.; Miner, Philippe; Lambert, C.; Le Goic, N.; Huvet, Arnaud; Quillien, Virgile; Boullot, F.; Amzil, Zouher; Hegaret, Helene; Fabioux, C.
Blooms of the dinoflagellate Alexandrium spp., known as producers of paralytic shellfish toxins (PSTs), are regularly detected on the French coastline. PSTs accumulate into harvested shellfish species, such as the Pacific oyster Crassostrea gigas, and can cause strong disorders to consumers at high doses. The impacts of Alexandrium minutum on C. gigas have often been attributed to its production of PSTs without testing separately the effects of the bioactive extracellular compounds (BECs) with allelopathic, hemolytic, cytotoxic or ichthyotoxic properties, which can also be produced by these algae. The BECs, still uncharacterized, are excreted within the environment thereby impacting not only phytoplankton, zooplankton but also marine invertebrates and...
Tipo: Text
Palavras-chave: Harmful algal bloom (HAB); Crassostrea gigas; Paralytic shellfish toxins (PST); Bioactive extracellular compounds (BEC); Histology; Behavior.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00435/54637/56042.pdf

A Microarray-Based Analysis of Gametogenesis in Two Portuguese Populations of the European Clam Ruditapes decussatus
Provedor de dados: 5
Autores: Sousa, Joana; Milan, Massimo; Bargelloni, Luca; Pauletto, Marianna; Matias, Domitilia; Joaquim, Sandra; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud.
The European clam, Ruditapes decussatus is a species with a high commercial importance in Portugal and other Southern European countries. Its production is almost exclusively based on natural recruitment, which is subject to high annual fluctuations. Increased knowledge of the natural reproductive cycle of R. decussatus and its molecular mechanisms would be particularly important in providing new highly valuable genomic information for better understanding the regulation of reproduction in this economically important aquaculture species. In this study, the transcriptomic bases of R. decussatus reproduction have been analysed using a custom oligonucleotide microarray representing 51,678 assembled contigs. Microarray analyses were performed in four gonadal...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00188/29935/28623.pdf

Tracing key genes associated with the Pinctada margaritifera albino phenotype from juvenile to cultured pearl harvest stages using multiple whole transcriptome sequencing
Provedor de dados: 5
Autores: Auffret, Pauline; Le Luyer, Jeremy; Sham Koua, Manaarii; Quillien, Virgile; Ky, Chin-long.
Background Albino mutations are commonly observed in the animal kingdom, including in bivalves. In the black-lipped pearl oyster Pinctada margaritifera, albino specimens are characterized by total or partial absence of colouration resulting in typical white shell phenotype expression. The relationship of shell colour with resulting cultured pearl colour is of great economic interest in P. margaritifera, on which a pearl industry is based. Hence, the albino phenotype provides a useful way to examine the molecular mechanisms underlying pigmentation. Results Whole transcriptome RNA-sequencing analysis comparing albino and black wild-type phenotypes at three stages over the culture cycle of P. margaritifera revealed a total of 1606, 798 and 187 differentially...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Albinism; Pigmentation; RNA-seq; Notch signaling pathway; Tyrosinase; Biomineralization.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00652/76420/77477.pdf

A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus
Provedor de dados: 5
Autores: De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud.
A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment...
Tipo: Text
Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00260/37147/35676.pdf

Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Normand, Julien; Li, Ronghua; Quillien, Virgile; Nicolas, Jean-louis; Boudry, Pierre; Pernet, Fabrice; Huvet, Arnaud.
Pacific oyster Crassostrea gigas suffers from chronic or sporadic mortality outbreaks worldwide, resulting from infectious diseases and/or physiological disorders triggered by environmental factors. Since 2008, ostreid herpesvirus OsHV-1 μVar has been identified as the main agent responsible for mass mortality of juvenile oysters in Europe. Previous studies of genome-wide expression profiling have provided candidate genes that potentially contribute to genetically-based resistance to summer mortality. To assess their value in determining resistance to the juvenile mass mortality that has occurred in France since 2008, we analyzed the expression of 17 candidate genes in an experimental infection by OsHV-1 μVar, and in an in vivo field experiment. Individual...
Tipo: Text
Palavras-chave: Molluscs; Gene expression profiling; Inhibitor of kappa B; Summer mortality; Ostreid herpesvirus.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00177/28823/27325.pdf

Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas
Provedor de dados: 5
Autores: Guevelou, Eric; Huvet, Arnaud; Galindo-sanchez, Clara E.; Milan, Massimo; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the...
Tipo: Text
Palavras-chave: AMP-activated protein kinase; Crassostrea gigas; Gametogenesis; Marine bivalve; Reproduction.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00168/27911/26470.pdf

Phenome of pearl quality traits in the mollusc transplant model Pinctada margaritifera
Provedor de dados: 5
Autores: Ky, Chin-long; Quillien, Virgile; Broustal, Floriane; Soyez, Claude; Devaux, Dominique.
The bivalve Pinctada margaritifera exhibits three main transplant phenotypes derived from  the donor (from which a mantle graft tissue, the saibo, is excised), the recipient (into which  the saibo is implanted with a nucleus, leading to the formation of a pearl sac "chimera") and  the cultured pearls themselves. This first phenome study on the species derived from a large  experimental graft. Transplant phenotype was assessed at three scales: 1) macro, pearl size,  colour, grade, 2) micro, pearl surface microstructure, and 3) molecular, biomineralisation  gene expression level in saibo and pearl sac tissues. From donor to pearl, the phenome  revealed fine variations of quality traits dependent on the position on the mantle where the  saibo was cut, whose...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53419/54306.pdf

The effects of eight single microalgal diets on sex-ratio and gonad development throughout European flat oyster (Ostrea edulis L.) conditioning
Provedor de dados: 5
Autores: Gonzales, Ricardo; Quillien, Virgile; Robert, Rene.
To determine the effects of food quality on Ostrea edulis reproduction, European flat oysters were conditioned during two sets of experiments, carried out in spring and autumn, during 40 days at 19 degrees C, in 50 l transparent flow-through tanks, in triplicate, and fed constantly at 900 mu m(3) mu l(-1), with eight different types of microalgae. Four species were fed per group of trials: Isochrysis affinis galbana, Chaetoceros gracilis, Skeletonema marinoi, and Tetraselmis suecica were fed to flat oysters in the first set; whereas Rhodomonas salina, Thalassiosira weissflogii, Thalassiosira pseudonana, and Pavlova lutheri were provided during the second set of experiments. At the beginning and end of both conditioning periods, oysters were sampled and...
Tipo: Text
Palavras-chave: Ostrea edulis; Broodstock conditioning; Gametogenesis; Microalgal diets.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00144/25497/24163.pdf

Disruption of amylase genes by RNA interference affects reproduction in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Beguel, Jean-philippe; Pereira Cavaleiro, Nathalia; Thomas, Yoann; Quillien, Virgile; Boudry, Pierre; Alunno-bruscia, Marianne; Fabioux, Caroline.
Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15...
Tipo: Text
Palavras-chave: Alpha-Amylase; DsRNA; Gametogenesis; Marine bivalve; Dynamic energy budget.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00271/38239/36422.pdf

Microplastics Affect Energy Balance and Gametogenesis in the Pearl Oyster     Pinctada margaritifera
Provedor de dados: 5
Autores: Gardon, Tony; Reisser, Celine; Soyez, Claude; Quillien, Virgile; Le Moullac, Gilles.
Plastic pollution in the environment is increasing at global scale. Microplastics (MP) are derived from degradation of larger plastic items or directly produced in microparticles form (&lt;5 mm). Plastics, widely used in structures and equipments of pearl farming, are a source of pollution to the detriment of the lagoon ecosystem. In order to evaluate the impact of MP on the physiology of Pinctada margaritifera, a species of ecological and commercial interests, adult oysters were exposed to polystyrene microbeads (micro-PS of 6 and 10 μm) for 2 months. Three concentrations were tested: 0.25, 2.5, 25 μg L-1 and a control. Ingestion and respiration rate and assimilation efficiency were monitored on a metabolic measurement system to determine the individual...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00435/54684/56104.pdf

Reproductive effort of Pacific oysters: A trait associated with susceptibility to summer mortality
Provedor de dados: 5
Autores: Huvet, Arnaud; Normand, Julien; Fleury, Elodie; Quillien, Virgile; Fabioux, Caroline; Boudry, Pierre.
Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment and their pathogens. The physiological status of an oyster, especially its reproductive status, is suspected to play a significant role in the outcome of this interaction. As genetic variability exists for susceptibility to summer mortality, resistant (R) and susceptible (S) oyster lines were produced using a divergent selection scheme. The present paper reports a histological study on gonad area, which is representative of reproductive effort, in randomly chosen five R and five S oyster lines. The R lines showed a significantly lower gonad area than the S lines (P&lt;0.001). with an estimated mean difference of 12.5%, whereas,...
Tipo: Text
Palavras-chave: Bivalve mollusc; Crassostrea gigas; Reproduction; Selected lines; Summer mortality.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11766/8502.pdf

Whole transcriptome sequencing and biomineralization gene architecture associated with cultured pearl quality traits in the pearl oyster, Pinctada margaritifera
Provedor de dados: 5
Autores: Le Luyer, Jeremy; Auffret, Pauline; Quillien, Virgile; Leclerc, Nicolas; Reisser, Celine; Vidal-dupiol, Jeremie; Ky, Chin-long.
Background Cultured pearls are unique gems produced by living organisms, mainly molluscs of the  Pinctada genus, through the biomineralization properties of pearl sac tissue.  Improvement of P. margaritifera pearl quality is one of the biggest challenges that  Polynesian research has faced to date. To achieve this goal, a better understanding of the  complex mechanisms related to nacre and pearl formation is essential and can now be  approached through the use of massive parallel sequencing technologies. The aim of this  study was to use RNA-seq to compare whole transcriptome expression of pearl sacs that  had producing pearls with high and low quality. For this purpose, a comprehensive  reference transcriptome of P. margaritifera was built based on...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Biomineralization; Pearl quality; Pearl sac; RNA-seq.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00475/58695/61207.pdf

Evaluation of the gonad volume throughout magnetic resonance imaging in Crassostrea gigas and comparison with the histological method
Provedor de dados: 5
Autores: Flahauw, Emilie; Davenel, Armel; Quellec, Stéphane; Quillien, Virgile; Degremont, Lionel; Lapegue, Sylvie; Hatt, Philippe-jacques.
Biological parameters of the cupped oyster Crassostrea gigas such as growth, reproductive effort (duration of the gametogenesis, date of spawning, and fecundity) differ widely among oysters in the same location. Furthermore, most of the traditional methods, like histological observations, could lead to large variability on the observations and the measurements ofthese traits, and large uncertainties on their changes over time. These can be drastically reduced by using Magnetic Resonance Imaging (MRI) which is a non invasive method allowing to measure the same individual during its development. Forty oysters were assessed using MRI for the volume of whole soft body, the intra valves space, the adductor muscle, and the gonad, which also include the digestive...
Tipo: Text
Palavras-chave: Molluscs; Crassostrea gigas; Gonad; Histology; MRI.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00107/21784/19370.pdf

Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication
Provedor de dados: 5
Autores: Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud.
Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters...
Tipo: Text
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf

Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment
Provedor de dados: 5
Autores: Beguel, Jean-philippe; Huvet, Arnaud; Quillien, Virgile; Lambert, Christophe; Fabioux, Caroline.
Energy allocation principle is a core element of life-history theory in which "the cost of reproduction" corresponds to an acceleration of senescence caused by an increase in reproductive investment. In the "theory of aging", senescence is mainly due to the degradation of lipids, proteins and DNA by reactive oxygen species (ROS), by-products of oxidative metabolism. Some studies have shown that oxidative stress susceptibility could be a cost of reproduction. The present study investigates the effect of reproductive investment on antioxidant capacity in the gills of a species with a very high reproductive investment, the Pacific oyster Crassostrea gigas. We used RNA interference targeting the oyster vasa-like gene (Oyvlg) to produce oysters with contrasted...
Tipo: Text
Palavras-chave: Antioxidant capacity; Crassostrea gigas; Reproduction; RNA interference; ROS.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00116/22744/21494.pdf

Oyster reproduction is affected by exposure to polystyrene microplastics
Provedor de dados: 5
Autores: Sussarellu, Rossana; Suquet, Marc; Thomas, Yoann; Lambert, Christophe; Fabioux, Caroline; Pernet, Marie Eve Julie; Le Goic, Nelly; Quillien, Virgile; Mingant, Christian; Epelboin, Yanouk; Corporeau, Charlotte; Guyomarch, Julien; Robbens, Johan; Paul-pont, Ika; Soudant, Philippe; Huvet, Arnaud.
Plastics are persistent synthetic polymers that accumulate as waste in the marine environment. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Because filter-feeder organisms ingest MP while feeding, they are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (micro-PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 0.023 mg·L−1) for 2 mo during a reproductive cycle. Effects were investigated on ecophysiological parameters; cellular, transcriptomic, and proteomic responses; fecundity; and offspring development. Oysters preferentially ingested the 6-µm micro-PS...
Tipo: Text
Palavras-chave: Microplastic; Reproduction; Energy allocation; Oyster.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00311/42233/41600.pdf

Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Boulais, Myrina; Corporeau, Charlotte; Huvet, Arnaud; Bernard, Ismael; Quere, Claudie; Quillien, Virgile; Fabioux, Caroline; Suquet, Marc.
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oocyte quality; Trochophore; Lipid; Gene expression; ATP.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00239/35010/33544.pdf

Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane.
To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to...
Tipo: Text
Palavras-chave: Ingestion; Gene expression; Bivalve; Alpha amylase.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-5165.pdf

Microplastics induce dose-specific transcriptomic disruptions in energy metabolism and immunity of the pearl oyster Pinctada margaritifera
Provedor de dados: 5
Autores: Gardon, Tony; Morvan, Lucie; Huvet, Arnaud; Quillien, Virgile; Soyez, Claude; Le Moullac, Gilles; Le Luyer, Jeremy.
A combined approach integrating bioenergetics and major biological activities is essential to properly understand the impact of microplastics (MP) on marine organisms. Following experimental exposure of polystyrene microbeads (micro-PS of 6 and 10 μm) at 0.25, 2.5, and 25 μg L−1, which demonstrated a dose-dependent decrease of energy balance in the pearl oyster Pinctada margaritifera, a transcriptomic study was conducted on mantle tissue. Transcriptomic data helped us to decipher the molecular mechanisms involved in P. margaritifera responses to micro-PS and search more broadly for effects on energetically expensive maintenance functions. Genes related to the detoxification process were impacted by long-term micro-PS exposure through a decrease in...
Tipo: Text
Palavras-chave: Microbeads; Marine bivalve; Differentially expressed genes; Stress response; Energy-limited tolerance.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00640/75213/75351.pdf

Donor effect on cultured pearl nacre development and shell matrix gene expression in Pinctada margaritifera reared in different field sites
Provedor de dados: 5
Autores: Ky, Chin-long; Broustal, Floriane; Koua, Manaarii Sham; Quillien, Virgile; Beliaeff, Benoit.
Understanding the respective roles played by donor and recipient pearl oysters in pearl quality determination in relation to the environment is a challenge for the pearl industry. In most Pinctada species, pearl size is mainly related to recipient oyster growth performance, but also relies to some extent on the biomineralisation activity of the pearl sac, a tissue that originates from the donor oyster mantle. We examined donor effect on pearl size in response to culture in the lagoons on Arutua and Apataki atolls. Overall nacre weight and thickness were greater in Arutua than in Apataki, but sensitivity to the environment differed between donors. Some donors were associated with significantly heavier and thicker nacre in Arutua (I group), while others had...
Tipo: Text
Palavras-chave: Biomineralisation; Environment; Pearl oyster; Pearl size; Pinctada margaritifera.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00425/53699/54610.pdf

In situ markers of Crassostrea gigas oyster sensibility to pathogen infection : toward the selection of early indicators of summer mortality
Provedor de dados: 5
Autores: Fleury, Elodie; Normand, Julien; Suquet, Eloïse; Dechamps, Lucie; Quillien, Virgile; Richard, Marion; Cochennec-laureau, Nathalie.
Massive mortalities of Crassostrea gigas spat associated with OsHV-1 virus occurred every year since 2008, all along the French coasts. Previous experimental studies lead to identify physiological functions of the oyster metabolism that responds to infection, denoting infection routes and symptoms or indicating immune response. Among these functions, studies highlighted several genes whose expression was significantly down or upregulated before the mortality event. The expression of these genes should be then used as predictive indicator of oyster response to pathogen infection, including marker-associated selection. In this study, two experiments were designed to identify genes linked to early infection response of Pacific oyster spat during in situ...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00286/39768/38261.pdf