Consulta: creatorFacets:"Renault, Tristan"
Registros recuperados: 225
Data/hora: 11/06/2024 14:35:20

Compte-rendu des journées de la surveillance de la santé des mollusques marins 2013
Provedor de dados: 5
Autores: Garcia, Celine; Francois, Cyrille; Renault, Tristan; Lupo, Coralie.
Les 12 et 13 novembre 2013, les journées de la surveillance de la santé des mollusques marins ont rassemblé les acteurs de la santé des mollusques marins (Ifremer, DGAl, DPMA, DDTM, CNC, CRC, laboratoires d'analyses agréés et reconnus, centres techniques). Les objectifs de cette réunion étaient de faire un bilan des activités 2013 du Laboratoire National de Référence (LNR), des réseaux de surveillance (REPAMO) et d'Observation (RESCO et réseaux de centres techniques).
Tipo: Text
Palavras-chave: Réseau Surveillance; Mollusques marins; Maladie; Organismes pathogènes; Détection.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00170/28146/26371.pdf

Lymphocystis outbreaks in farmed sea bream, Sparus aurata, first report on French Mediterranean coast
Provedor de dados: 5
Autores: Le Deuff, Rose-marie; Renault, Tristan.
Among the Iridovirus related infections frequently described on many teleosts fish species, Viral Erythrocytic Necrosis (VEN) (Pinto et al., 1989), Epizootic Haematopoietic Necrosis Virus (EHNV) (Langdon et al., 1988) as weil as Lymphocystis Disease Virus (LDV) are not considered to directly cause fatality. Indeed, Lymphocystis outbreaks of farmed fish could be observed in some cases, as a consequence of a bacterial double infection or cannibalism (Ahne ef al., 1991; Moate et al., 1992). Here, we report for the first time a strong LDV infection, and an additional physical stress responsible for mortalities of reared sea bream, Sparus aurata, from Toulon area, on French Mediterranean coast. Although, since the first report of Lymphocystis in sea bream under...
Tipo: Text
Palavras-chave: Mortalities; Sparus aurata; Fish; Iridovirus.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/1993/publication-2886.pdf

Rapid and sensitive detection of ostreid Herpesvirus 1 in oyster samples by real-time PCR (Poster)
Provedor de dados: 5
Autores: Pepin, Jean-francois; Riou, Antoine; Renault, Tristan.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region, which is present in two copies in the OsHV-1 genome and encodes two proteins of unknown function. The assay was applied successfully to rapid diagnosis (100 minutes)...
Tipo: Text
Palavras-chave: Crassostrea gigas; PCR; Oyster; OsHV 1; Detection; Herpes virus 1.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3653.pdf

Molecular responses of Ostrea edulis haemocytes to an in vitro infection with Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms.  Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf

Les apports de la génétique dans la filière ostréicole française
Provedor de dados: 5
Autores: Lapegue, Sylvie; Renault, Tristan.
The most known and certainly the most important contribution of genetics to the oyster indus try is the development of the triploid cupped oyster. Released for the first time in the 1980s, the triploid oyster has triplets of  chromosomes instead of pairs. Currently, triploid oysters are obtained by crossing diploid and tetraploid oysters. These oysters are characterized by a near lack of milt in summer, which makes them generally popular with consumers, and by a faster growth, allowing a shorter production cycle. In parallel, the exploitation of natural resistance in the diversity of French oysters has been considered to improve the health of farmed populations which is a  major challenge for the sustainability of aquaculture farms. Currently, the...
Tipo: Text
Palavras-chave: Huître creuse; Triploïdie; Sélection; Maladies; Ostréiculture; Cupped oyster; Triploidy; Selection; Disease; Shellfish farming.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00498/60931/64326.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - Final Report 4th January 1999 to 3rd January 2002
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf

Infections à virus de type herpes chez les bivalves : large spectre d'hôte et détection au stade adulte
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan.
In 1972, Farley reported the presence of herpes-type viral particles in adult American oysters (Crassostrea virginica) for the first time. Since then, this pathogen has been detected in various marine bivalve species, usually associated with massive mortality events. To date, herpes-type viruses have been detected in the following species: C gigas, Ostrea edulis, O. angasi, Tiostrea chilensis, Ruditapes decussatus, R. philippinarum and most recently, Pecten maximus.
Tipo: Text
Palavras-chave: Pathogènes; Bivalves; Herpesvirus; Herpesviridae.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3266.pdf

Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1
Provedor de dados: 5
Autores: Segarra, Amelie; Mauduit, Florian; Faury, Nicole; Trancart, Suzanne; Degremont, Lionel; Tourbiez, Delphine; Haffner, Philippe; Barbosa-solomieu, Valerie; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
Background:  Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches.  Results:  The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant...
Tipo: Text
Palavras-chave: Interactions; Ostreid herpesvirus 1; Gene expression; Susceptibility; Crassostrea gigas.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00199/31029/29431.pdf

Bilan 1998 du réseau REPAMO - Réseau National de Surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Chollet, Bruno; Cochennec, Nathalie; Renault, Tristan.
Le réseau REPAMO répond à plusieurs objectifs, définis dans différentes Directives Européennes: prévenir et détecter l'introduction et la propagation d'un agent pathogène émergent, réémergent ou exotique pour les productions de coquillages d'intérêt commercial en France, surveiller l'évolution des maladies à déclaration obligatoire, surveiller les échanges avec nos partenaires commerciaux. Le fonctionnement repose sur la collaboration entre les professionnels, la DPMCM, les laboratoires côtiers d'IFREMER, et trois cellules d'analyses. Les prélèvements et les outils diagnostiques choisis dépendent des différents objectifs. Le suivi est fait sur des populations sauvages et cultivées du littoral français. Le nombre d'analyses progresse régulièrement grâce à...
Tipo: Text
Palavras-chave: Réseau surveillance; Pathologie; Mortalités; Mollusques marins.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00098/20904/18523.pdf

Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays
Provedor de dados: 5
Autores: Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan.
Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted...
Tipo: Text
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf

Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene
Provedor de dados: 5
Autores: Chen, M.h.; Kuo, S. T.; Renault, Tristan; Friedman, C. S.; Chang, P. H.
A 5781-base pair (bp) fragment of genomic DNA from the Taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the DNA polymerase gene of the abalone herpesvirus strain Victoria/AUS/2007. Homology of the amino acid sequence with the DNA polymerase of ostreid herpesvirus 1 was 30% (563/1856). In this study, a PCR-based procedure for detecting herpesvirus infection of abalone, Haliotis diversicolor supertexta, in Taiwan was developed. The method employed primer sets targeting the viral DNA polymerase gene, and was able to amplify DNA fragments of the expected size from infected samples. Primer sets of 40f and 146r were designed for amplification of an...
Tipo: Text
Palavras-chave: Herpes virus; Abalone; Haliotis diversicolor supertexta; DNA polymerase; Polymerase chain reaction.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20952/19675.pdf

Pathogens in aquaculture : specific pathogens or human contamination risks
Provedor de dados: 5
Autores: Renault, Tristan.
The vatness of aquaculture ventures worldwide has taken impressive leaps durint the past three decades, and a large portion of this pertains to mariculture. Significant strides have been made in the design and implementation of more efficient and cost effective growing areas, feed, and harvesting techniques. Moreover the economic impact of aquaculture will certainly increase in the years ahead.
Tipo: Text
Palavras-chave: Mycoplasmas; Chlamydiae; Rickettsia; Bacteria; Virus; Aquaculture; Pathogens.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2899.pdf

Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Thebault, Anne; Gerard, Andre.
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of...
Tipo: Text
Palavras-chave: Protein; DNA; Healthy carrier; Adult oyster; Crassostrea gigas; Herpesvirus.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-697.pdf

Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1
Provedor de dados: 5
Autores: Renault, Tristan; Faury, Nicole; Barbosa Solomieu, Valérie; Moreau, Kevin.
Virus-induced genes were identified using suppression subtractive hybridisation (SSH) from Pacific cupped oyster, Crassostrea gigas, haemocytes challenged by OsHV-1. A total of 304 clones from SSH forward library were sequenced. Among these sequences, some homologues corresponded to (i) immune related genes (macrophage express protein, IK cytokine, interferon-induced protein 44 or multicopper oxidase), (ii) apoptosis related genes (Bcl-2) and (iii) cell signalling and virus receptor genes (glypican). Molecular characterization and phylogenic analysis of 3 immune-related genes (macrophage expressed protein, multicopper oxidase and immunoglobulin domain cell adhesion molecule) were performed. Finally, quantitative PCR revealed significant changes in the...
Tipo: Text
Palavras-chave: Pacific cupped oyster; Crassostrea gigas; OsHV-1; Immunity; Gene expression; Multicopper oxidase; Macrophage expressed protein; Ig cell adhesion molecule; Interferon-induced protein 44; Virus RNA.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00043/15466/12843.pdf

Le Réseau REPAMO : un outil de surveillance de la santé des mollusques marins élevés et sauvages
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Arzul, Isabelle; Robert, Maeva; Omnes, Emmanuelle; Chollet, Bruno; Renault, Tristan.
The Ifremer Repamo network (REseau de PAthologie des MOllusques) is the national surveillance network in charge of monitoring marine mollusc health on French coastlines. It is commissioned to enforce the official regulations and to provide a public service, under the supervision of the competent authority (DGAL Veterinary Directorate) in the Ministry.of Agriculture and Fisheries. Its goals are to prevent the introduction and the dissemination of infectious agents, in particular pathogens involved in reportable diseases, and to monitor the changes in the pathogens already present on the French coasts.
Tipo: Text
Palavras-chave: Network; Surveillance; Mollusks; Pathogens; Regulations.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6946.pdf

Shellfish viruses
Provedor de dados: 5
Autores: Renault, Tristan.
Mortalities in a number of commercially important mollusc species have been associated with the detection of viruses belonging to several families. The first description of a virus was in adult eastern oyters, Crassostrea virginica, with the detection of virus particles resembling members of the family Herpesviridae. Subsequently, mass mortalities in French stocks of adult Portuguese oysters, C. angulata, were associated with irido-like virus infection. Other viruses observed in molluscs are described as members of the families Iridoviridae, Papovaviridae and Reoviridae. Little information is available on viral infections that affect molluscs due to primarily to the inadequacy of diagnostic methods that are employed when mass mortality events occur. Most...
Tipo: Text
Palavras-chave: Virus; Scallops; Picornavirus; Oyter herpesvirus 1; Oysters; Molluscs; Irido Like virus; Diseases; Herpesvirus; Clams; Bivalves; Birnavirus; Abalones.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4932.pdf

Etude de l'herpès virus de l'huître - Fonds d'Aide à la Recherche et à l'Innovation - Décision d'Aide à la Recherche n° 95-07-01 - Rapport intermédiaire 1998
Provedor de dados: 5
Autores: Renault, Tristan.
Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les outils de diagnostic développés sont basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR). Ce projet, proposé sur une durée de trois années (1995-1998),...
Tipo: Text
Palavras-chave: Virus; Pathologie; Herpès virus; Crassostrea gigas; Pathogène; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15450/12825.pdf

Herpesviruses associated with mortalities among Pacific oyster, Crassostrea gigas, in France-Comparative study
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Maffart, Patricia.
Sporadic mortalities were reported in june and july of 1992 and 1993 among batches of hatchery-reared larval Pacifie oyster, Crassastrea gigas, and at the beginning of July and in August of 1993 among five batches of 3-7 month old young spats, C. gigas. Observations with transmission electron microscope showed the presence of herpes-like virus particles in infected larvae and young spats. The viruses observed in diseased larvae and affected young spat oysters were very similar. Elevated temperature and crowding may increase susceptibility of oysters to these herpes-like virus infections.
Tipo: Text
Palavras-chave: Spat; Larvae; Crassostrea gigas; Pacific oysters; Mortality; Herpes like virus; Naissain; Larve; Crassostrea gigas; Huître japonaise; Mortalités; Herpès like virus.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-2898.pdf

In situ localization and tissue distribution of ostreid herpesvirus 1 proteins in infected Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Martenot, Claire; Segarra, Amelie; Baillon, Laury; Faury, Nicole; Houssin, Maryline; Renault, Tristan.
Immunohistochemistry (IHC) assays were conducted on paraffin sections from experimentally infected spat and unchallenged spat produced in hatchery to determine the tissue distribution of three viral proteins within the Pacific oyster, Crassostrea gigas. Polyclonal antibodies were produced from recombinant proteins corresponding to two putative membrane proteins and one putative apoptosis inhibitor encoded by ORF 25, 72, and 87, respectively. Results were then compared to those obtained by in situ hybridization performed on the same individuals, and showed a substantial agreement according to Landis and Koch numeric scale. Positive signals were mainly observed in connective tissue of gills, mantle, adductor muscle, heart, digestive gland, labial palps, and...
Tipo: Text
Palavras-chave: OsHV-1; Viral proteins; Crassostrea gigas.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00326/43697/43124.pdf

Pollutant effects on Pacific oyster, Crassostrea gigas (Thunberg), hemocytes: Screening of 23 molecules using flow cytometry
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan.
The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed...
Tipo: Text
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf

Surveillance du milieu marin et protection sanitaire des coquillages (Acte)
Provedor de dados: 5
Autores: Renault, Tristan.
Les mollusques sont à la base d'une activité économique importante au sein de l'Union Européenne, la France étant un des premiers pays producteurs. La conchyliculture doit répondre aujourd'hui aux attentes de la société en termes de sécurité alimentaire. Des réglementations ont ainsi été établies, tao! au niveau des étapes primaires de pêche, d'élevage ou de récolte des mollusques, que lors de la transformation et de la commercialisation. Au cours des étapes de production et de récolte, une contamination des coquillages (toxines algales, agents infectieux ou polluants) peut survenir du fait, notamment, des conditions environnementales auxquelles sont confrontés les animaux. Des réseaux de surveillance ont ainsi été développés afin de détecter la présence...
Tipo: Text
Palavras-chave: Polluants; Contamination microbiologique; Algues toxiques; Coquillages; Milieu marin; Réseaux; Surveillance.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/acte-6933.pdf

Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat
Provedor de dados: 5
Autores: Segarra, Amelie; Baillon, Laury; Faury, Nicole; Tourbiez, Delphine; Renault, Tristan.
High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral...
Tipo: Text
Palavras-chave: Crassostrea gigas; In situ hybridization; Ostreid herpesvirus 1; Viral DNA; Viral RNA.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00300/41099/40272.pdf

A study of autophagy in hemocytes of the Pacific oyster,   Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...
Tipo: Text
Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf

Characterization of summer mortalities of C. gigas oyster in France in relation to environmental parameters
Provedor de dados: 5
Autores: Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Costil, K. S.; Dubois, Brice; Degremont, Lionel; Bouget, Jean-francois; Martin, Jean-louis; Enriquez-diaz, M.; Faury, Nicole; Le Moine, Olivier; Renault, Tristan; Gagnaire, Beatrice; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois.
Field characterization of summer mortality was performed in France in the frame of the Morest project. Natural and hatchery spat were compared between three oyster production areas in France. Regardless of the natural or hatchery origin, oysters died during the reproduction period after temperature reaches 19 degree C. Thus, in southern areas, temperature accelerated gametogenesis of small spat (10mm) as well as adults, and mortality appeared for the two age classes. In contrast, sexual maturation proceeded more slowly in northern where spat mortality was lower compared to 18 months old oysters. However, critical gametogenesis and temperature were not sufficient to induce mortalities, as observed in examples with stable environment. Alternatively sediment...
Tipo: Text
Palavras-chave: Seasonal variation; Oyster culture; Mortality causes; Hatcheries; Gametogenesis; Environmental factors; Crassostrea gigas; Oysters.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3348.pdf

Vibrio damsela as a pathogenic agent causing mortalities in cultured sea bass (Lates calcarifer)
Provedor de dados: 5
Autores: Renault, Tristan; Haffner, Philippe; Malfondet, C.; Weppe, Maurice.
Vibrio anguillarum and Vibrio ordali are species frequently described as fish pathogens. Seven species of Vibrio can also be implicated in disease problems in mariculture (Toranzo  1990). sorne of Vibrios and Barja, In addition, these marine such as V. vulnificus (Tison et al.. 1982) and V. damsela (Love et al., 1981) can  also cause illness homoiothermic animals
Tipo: Text
Palavras-chave: Fish pathogens; Lates calacrifer; Mortality; Vibrio damsela.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-2889.pdf

Rapport d'activité 1993 de l'Unité de Recherche ne Pathologie et Immunologie Géénrales (URPIG) La Tremblade
Provedor de dados: 5
Autores: Renault, Tristan.
Rapport d'activité 1993 de l'Unité de Recherche en Pathologie et Immunologie Générales.
Tipo: Text
Palavras-chave: Pathologie; Immunologie; Invertébrés marins; Rapport Activité.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00071/18250/15813.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text
Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf

Autophagy in Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Moreau, Pierrick; Moreau, Kevin; Morga, Benjamin; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David; Renault, Tristan.
Mass mortality outbreaks of Pacific oysters, Crassostrea gigas, are reported in different areas around the world affecting seriously the shellfish aquaculture sector. Two pathogens are associated with these mortality outbreaks, ostreid herpesvirus 1 (OsHV-1) and Vibrio aestuarianus. In this contexte, a better knowledge is needed in terms of oyster immunity. For this purpose, as an important degradation pathway autophagy was investigated in the Pacific oysters. An in silico research of genes involved in autophagy using the C. gigas genome allowed identification of homologs of ATG1 and ATG8/LC3(Microtubule-associated protein 1A/1B-light chain 3). Gene expression and protein detection were analysed using real-time PCR and western blot, respectively. ATG1 and...
Tipo: Text
Palavras-chave: Autophagy; Pacific oyster; Crassostrea gigas; OsHV-1; Vibrio aestuarianus.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00218/32876/31345.pdf

Diploid and triploid Pacific oysters, Crassostrea gigas (Thunberg), reared at two heights above sediment in Marennes-Oleron Basin, France: Difference in mortality, sexual maturation and hemocyte parameters
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Soletchnik, Patrick; Madec, Patrick; Geairon, Philippe; Le Moine, Olivier; Renault, Tristan.
Summer mortality is an important economic concern for the Pacific oyster, Crassostrea gigas, industry all over the world and particularly in France. Outbreaks appear when oysters are in gonadal maturation. Bivalve defence mechanisms against pathogen invasion are assumed by circulating cells, the hemocytes. A field experiment was conducted in the Marennes-Oleron Basin (Charente-Maritime, France) in order to monitor hemocyte parameters, gametogenesis and mortality rates among C. gigas during 7 months from March to September 2002. Diploid (from natural bed and from hatchery) and triploid oysters were placed at 15 cm or 70 cm above sediment at Marennes-Oleron Basin. Triploid animals were included because they are considered as sterile animals. Mortality rates...
Tipo: Text
Palavras-chave: Sexual maturation; Summer mortality; Triploids; Diploids; Flow cytometry; Hemocyte activities; Crassostrea gigas; Pacific oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2923.pdf

Rapport sur les causes des mortalités d'huîtres creuses observées en juin 1992 sur le Banc de Ronce Les Bains (Bassin de Marennes-Oléron)
Provedor de dados: 5
Autores: Heral, Maurice; Bacher, Cedric; Razet, Daniel; Prou, Jean; Galgani, Francois; Renault, Tristan; Bodoy, Alain; Garnier, Jacqueline; Geairon, Philippe; Chagot, Dominique; Grizel, Henri; Comps, Michel; Masson, Daniel; Noinin, Michel.
Dès le 4 JUIN 1992, un ostréiculteur digne de confiance avait signalé aux services d'IFREMER, une situation anormale sur le banc de Ronce les Bains. Un premier prélèvement a été effectué le jour même. En effet, ce secteur avait déjà été le lieu de mortalités d'huitres estimées à 7 000 tonnes, au printemps 1988, et s'est révélé par le passé comme étant un endroit sensible. Pour information, figure en annexe le rapport établi en 1988, à destination du Préfet de la Charente Maritime. Ces mortalités ayant rapidement pris de l'ampleur, un dépôt de plainte contre X a été effectué par la Section Régionale de la Conchyliculture. A la suite de quoi, les services d'IFREMER ont été requis par la Gendarmerie Nationale (Brigade de Marennes) le 10 juin 1992, pour...
Tipo: Text
Palavras-chave: Ostréiculture; Huîtres creuses; Crassostrea gigas; Mortalité; Banc de Ronce Les Bains; Bassin de Marennes Oléron.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00048/15899/13327.pdf

Ostreid herpes virus 1 infection in families of the Pacific oyster, Crassostrea gigas, during a summer mortality outbreak: Differences in viral DNA detection and quantification using real-time PCR
Provedor de dados: 5
Autores: Sauvage, Christopher; Pepin, Jean-francois; Lapegue, Sylvie; Boudry, Pierre; Renault, Tristan.
Ostreid herpes virus 1 (OsHV-1) infections, notably reported in Europe and the USA, are closely associated with significant mortalities of the Pacific oyster, Crassostrea gigas, especially during its early stages of life. In summer 2006, we monitored mortality by strict daily verification of three full-sib families of oysters reared under common conditions. We quantified OsHV-1 using real-time PCR in dead and living individuals during and after a mortality event. Mortality events were severe and brief, but significantly different between tested families (cumulative mortality ranging from 1.2 to 49%). Real-time PCR assays revealed different viral DNA loads in dead individuals from different families (P &lt; 0.001). Moreover, the mean level of infection...
Tipo: Text
Palavras-chave: Viral DNA quantification; Real time PCR; OsHV 1; Mortality; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6265.pdf

Ostreid Herpesvirus 1 Infection among Pacific Oyster (Crassostrea gigas) Spat: Relevance of Water Temperature to Virus Replication and Circulation Prior to the Onset of Mortality
Provedor de dados: 5
Autores: Renault, Tristan; Bouquet, Anne Lise; Maurice, Julien-thomas; Lupo, Coralie; Blachier, Philippe.
number of bivalve species worldwide, including the Pacific oyster, Crassostrea gigas, have been affected by mass mortality events associated with herpesviruses, resulting in significant losses. A particular herpesvirus was purified from naturally infected larval Pacific oysters, and its genome was completely sequenced. This virus has been classified as Ostreid herpesvirus 1 (OsHV-1) within the family Malacoherpesviridae. Since 2008, mass mortality outbreaks among C. gigas in Europe have been related to the detection of a variant of OsHV-1 called µVar. Additional data are necessary to better describe mortality events in relation to environmental-parameter fluctuations and OsHV-1 detection. For this purpose, a single batch of Pacific oyster spat was deployed...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00207/31871/30320.pdf

La Bonamiose : études des mécanismes cellulaires de défense de l'huître plate, Ostrea edulis, vis à vis du parasite Bonamia ostreae
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Chollet, Bruno.
Bonamiosis—a disease caused by a protozoan parasite, Bonamia ostreae—was detected for the first time in Brittany in 1979 in conjunction with a massive mortality event. Since then, the disease has spread to all French oyster farming areas, and then throughout Europe.
Tipo: Text
Palavras-chave: Pathologie; Ostrea edulis; Bonamia ostreae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3241.pdf

Commentaire de l'Ifremer sur le projet de manuel de diagnostic et de surveillance pour les animaux aquatiques
Provedor de dados: 5
Autores: Garcia, Celine; Lapegue, Sylvie; Renault, Tristan.
Tipo: Text
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00217/32853/80979.pdf

Défi MOREST - Mortalités estivales de l'huître creuse Crassostrea gigas - Caractérisation des facteurs de risques associés aux mortalités estivales - Synthèse du thème 5 - Risque associé aux pathogènes. (La Rochelle 14-15 mars 2006)
Provedor de dados: 5
Autores: Nicolas, Jean-louis; Renault, Tristan; Gagnaire, Beatrice; Garcia, Celine; Le Roux, F; Miossec, Laurence; Pepin, Jean-francois; Saulnier, Denis.
L'un des objectifs principaux des travaux en pathologie des mollusques est d'identifier et d'étudier des agents infectieux capables d'induire des maladies et des mortalités chez les coquillages, en particulier, chez I'huître creuse, Crassostrea gigas, espèce principalement exploitée en France. Ces travaux semblent d'autant plus indispensables qu'il n'existait pas, jusqu'à ces dernières années, de maladies identifiées chez I'huître creuse en Europe excepté le virus herpès suspecté dès 1995 de provoquer des mortalités de naissain (Renault et al, 1995). Les principales maladies des espèces proches sont dues à des parasites comme Haplosporidium nelsoni (Ford et Heskin, 1987) et Perkinsus marinus Olivier et al, 1 998) pour Crassostrea virginica, Marteilia...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00304/41535/40743.pdf

Viruses infecting marine molluscs
Provedor de dados: 5
Autores: Arzul, Isabelle; Corbeil, Serge; Morga, Benjamin; Renault, Tristan.
Although a wide range of viruses have been reported in marine molluscs, most of these reports rely on ultrastructural examination and few of these viruses have been fully characterized. The lack of marine mollusc cell lines restricts virus isolation capacities and subsequent characterization works. Our current knowledge is mostly restricted to viruses affecting farmed species such as oysters Crassostrea gigas, abalone Haliotis diversicolor supertexta or the scallop Chlamys farreri. Molecular approaches which are needed to identify virus affiliation have been carried out for a small number of viruses, most of them belonging to the Herpesviridae and birnaviridae families. These last years, the use of New Generation Sequencing approach has allowed increasing...
Tipo: Text
Palavras-chave: Molluscs; Viruses; Diseases; Herpesviridae; Bivalve; Abalone.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00371/48206/48319.pdf

Impact of diuron on aneuploidy and hemocyte parameters in Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Bonnard, Marc; Gagnaire, Beatrice; Renault, Tristan; Lapegue, Sylvie.
Diuron is a substituted urea herbicide used for agricultural and nonagricultural weed control. Its widespread use and relatively slow breakdown led us to analyze its influence on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in Pacific oysters, Crassostrea gigas. Adult oysters were subjected to two diuron concentrations (300 ng L-1 and 3 mu g L-1) for 11 weeks. Significantly higher aneuploidy level was observed in diuron-treated oysters compared with the control. Furthermore, the observed impact on aneuploidy persisted to the next generation as offspring exhibited significantly higher aneuploidy levels when their parents had been exposed to diuron. Significant increases in hemocyte parameters (cell...
Tipo: Text
Palavras-chave: Diuron; Herbicide impact; Cytometry; Hemocyte; Aneuploidy; Crassostrea gigas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2178.pdf

Environment and immunomodulation : effects of the soluble fraction of Norwegian heavy fuel on the immune system of Pacifc oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Bado-nilles, Anne; Auffret, Michel; Quentel, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood. The aim of the present work was to detect the impact in vivo of PAHs on the immune...
Tipo: Text
Palavras-chave: PaHs; Contamination; Crassostrea gigas; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3428.pdf

Effets d'un mélange de pesticides sur les hémocytes d'huître creuse, Crassostrea gigas.
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Geret, Florence; Haure, Joel; Martin, Jean-louis; Burgeot, Thierry; Thomas-guyon, Hélène; Renault, Tristan.
Shellfish industry is often realised in estuarine areas, subjected to pollutions due to agricultural activities. The harmful effects of pesticides on animals inhabiting these estuarine zones are poorly documented. Bivalve molluscs represent an interesting model because they are sedentary and they filter water intensively. Effects of pesticides on haemocytes functions were tested in Pacific oyster, Crassostrea gigas and analysed by flow cytometry. A combination of pesticide at environmental concentrations was tested in vivo. Animals used corresponded to two genetically selected populations produced in hatchery.
Tipo: Text
Palavras-chave: Haemocytes; Flow cytometry; Pesticides; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-924.pdf

Viruses of bivalve shellfish
Provedor de dados: 5
Autores: Renault, Tristan.
Bivalve molluscs are filter feeders and as a consequence they may bioaccumulate in their tissues viruses that infect humans and higher vertebrates. However, there have also been described mortalities of bivalve molluscs associated with viruses belonging to different families. Mass mortalities of adult Portuguese oysters, Crassostrea angulata, among French livestocks (between 1967 et 1973) were associated with irido-like virus infections. Herpesviruses were reported in the eastem oyster, Pacific oyster and European flat oyster and lately in scallops and clams. Disseminated neoplasia, a proliferative cell disorder of the circulatory system of bivalves, although of uncertain aetiology, has been suggested to be caused by retroviral infections. Other viruses...
Tipo: Text
Palavras-chave: Infection; Virus; Mollusc; Bivalve; Infection; Virus; Mollusque; Bivalve.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2925.pdf

Le réseau Repamo : un outil de surveillance de la santé des mollusques marins élevés et sauvages (acte)
Provedor de dados: 5
Autores: Francois, Cyrille; Miossec, Laurence; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Robert, Maeva; Ferrand, Sylvie; Chollet, Bruno; Renault, Tristan.
Created in 1992, the Repamo network (REseau de PAthologie des MOllusques – Mollusc Pathology Network) is in charge of the surveillance and monitoring of the marine molluscs health status along the French coast. It has a regulatory mission (European legislation 91/67 and 95/70) as well as a public service activity under the supervision of the Ministry of Agriculture and Fisheries through the DPMA. It aims at preventing the introduction and propagation of pathogenic agents, in particular the noticeable agents, and monitoring the evolution of those already present on the national territory. These activities are part of the institutional missions of the Ifremer. The missions of the network are the following: 1) surveillance of the evolution of noticeable...
Tipo: Text
Palavras-chave: Mortalités; Maladies; Pathologie; Mollusques; Réseau surveillance; REPAMO.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3715.pdf

Study of interactions between flat oyster, Ostrea edulis, and the parasite Bonamia ostreae using flow cytometry and suppression subtractrive hybridization (SSH)
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Gagnaire, Beatrice; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. Control of bonamiosis requires a good knowledge of the parasite life cycle including host-parasite interactions. In this study, we investigated interactions between parasites and haemocytes by flow cytometry and Suppression Subtractive Hybridization (SSH). In both approaches, analyses were performed after 2 hours of contact between haemocytes and parasites purified from highly infected flat oysters. Flow cytometry analyses consisted in testing haemocyte activities including esterase activities, reactive oxygen species (ROS) production and phagocytosis after contact with live parasites and parasites inactivated by...
Tipo: Text
Palavras-chave: SSH; Suppression subtractive hybridization; Cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3367.pdf

Autophagy plays an important role in protecting Pacific oysters from OsHV-1 and Vibrio aestuarianus infections
Provedor de dados: 5
Autores: Moreau, Pierrick; Moreau, Kevin; Segarra, Amelie; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David C.; Renault, Tristan.
Recent mass mortality outbreaks around the world in Pacific oysters, Crassostrea gigas, have seriously affected the aquaculture economy. Although the causes for these mortality outbreaks appear complex, infectious agents are involved. Two pathogens are associated with mass mortality outbreaks, the virus ostreid herpesvirus 1 (OsHV-1) and the bacterium Vibrio aestuarianus. Here we describe the interactions between these 2 pathogens and autophagy, a conserved intracellular pathway playing a key role in innate immunity. We show for the first time that autophagy pathway is present and functional in Pacific oysters and plays an important role to protect animals from infections. This study contributes to better understand the innate immune system of Pacific...
Tipo: Text
Palavras-chave: Autophagy; Crassostrea gigas; OsHV-1; Pacific oyster; Vibrio aestuarianus; ATG; Autophagy-related; Atg8-PE; Atg8-phosphatidylethenolamine; DNA; Deoxyribonucleic acid; Hpi; Hours postinfection; LC3-II; Cleaved; Lipidated and autophagosome-associated form of LC3; MAP1LC3A; B (LC3A; B); Microtubule-associated proteins 1 light chain 3 alpha.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/37989/36072.pdf

Summer mortality outbreaks of French Pacific oysters Crassostrea gigas in 2008 and 2009
Provedor de dados: 5
Autores: Renault, Tristan; Allain, Gwenhael; Arzul, Isabelle; Chollet, Bruno; Cobret, Laetitia; De Decker, Sophie; Faury, Nicole; Ferrand, Sylvie; Francois, Cyrille; Garcia, Celine; Haffner, Philippe; Joly, Jean-pierre; Miossec, Laurence; Morga, Benjamin; Nicolas, Jean-louis; Omnes, Emmanuelle; Pepin, Jean-francois; Saulnier, Denis; Schikorski, David; Segarra, Amelie.
Abnormal mortality outbreaks occurred in the majority of Pacific oyster (Crassostrea gigas) producing areas in France in 2008 during the summer period. These outbreaks were usually sudden and severe (up to 100%) and affected essentially spat (oysters less than 1 year-old) and juveniles (12 to 18 month-old oysters). Crassostrea gigas was the single affected species Mortality outbreaks were first observed from mid May to mid June in Brittany, Vendée, Charente Maritime and Mediterranean coast A second episode of massive mortality events was reported at the end of June and the beginning of July. All oyster production sites were affected except some particular locations including Arcachon Bay and Brittany. Mortality occurred latter in Arcachon Bay in August....
Tipo: Text
Palavras-chave: Abnormal mortality; Oysters; Crassostrea gigas; Pathogens.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00014/12554/9420.pdf

Mass mortalities in hatchery-reared sea bass (Lates calcarifer) larvae associated with the presence in the brain and retina of virus-like particles
Provedor de dados: 5
Autores: Renault, Tristan; Haffner, Philippe; Baudin Laurencien, F.; Breuil, Gilles; Bonami, Jean-robert.
Since 1986, an unknown disease causing high mortalities appeared in Ifremer's Pacific OceanologicaI Center (Tahiti) nursery of sea bass (Lates calcarifer). opathology of lesions involving both centraI nervous system (CNS) and eye retina, and the tuation exists in South-East Asian countries (Indonesia, Philippines, Malaysia and Singapore), and in northem Australia.  This paper describes the histmorphology of a new fish virus, which is believed to be the aetiological agent of high mortalities among sea bass larvae.
Tipo: Text
Palavras-chave: Virus like; Lates calacrifer; Hatchery; Mortality.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/publication-2885.pdf

Haemocytes from Crassostrea gigas and OsHV-1: A promising in vitro system to study host/virus interactions
Provedor de dados: 5
Autores: Morga, Benjamin; Faury, Nicole; Guesdon, Stephane; Chollet, Bruno; Renault, Tristan.
Since 2008, mass mortality outbreaks associated with the detection of particular variants of OsHV-1 have been reported in Crassostrea gigas spat and juveniles in several countries. Recent studies have reported information on viral replication during experimental infection. Viral DNA and RNA were also detected in the haemolymph and haemocytes suggesting that the virus could circulate through the circulatory system. However, it is unknown if the virus is free in the haemolymph, passively associated at the surface of haemocytes, or able to infect and replicate inside these cells inducing (or not) virion production. In the present study, we collected haemocytes from the haemolymphatic sinus of the adductor muscle of healthy C. gigas spat and exposed them in...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00399/50997/51780.pdf

Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat
Provedor de dados: 5
Autores: Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco.
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides...
Tipo: Text
Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf

Etude de l'herpès virus de l'huître. Fonds d'Aide à la Recherche et à l'Innovation. Décision d'Aide à la Recherche n° 95-07-01 (Rapport intermédiaire)
Provedor de dados: 5
Autores: Renault, Tristan.
L'Unité de Recherche en Pathologie et Immunologie Générales (Station IFREMER de La Tremblade) se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occassionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années...
Tipo: Text
Palavras-chave: Virus; Herpès; Pathogène; Purification; Herpesviridae; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00043/15447/12818.pdf

Characterization of Vibrio isolated from Crassostrea gigas spat suffering summer mortality outbreaks (slides)
Provedor de dados: 5
Autores: Gay, Melanie; Renault, Tristan; Le Roux, Frédérique.
The pathogens related to summer mortality outbreaks are a herpes virus and two bacterial strains one belonging to Vibrio splendidus biovar II and the other to Vibrio splendidus spp. However, the feature pathogen/opportunist of these strains is still unknown. Several strains belonging to the genus Vibrio have been identified as pathogen for different mollusk species. In the context of the French program Morest, experiments of cohabitation have been used to demonstrate the potential presence of a transmissible infectious agent in batches of oysters suffering from summer mortality outbreaks. More than one hundred Vibrio strains have been isolated from these experiments. These strains have been phenotypically and genotypically characterized. Their virulence...
Tipo: Text
Palavras-chave: Vibrio splendidus; Vibrio lentus; Crassostrea gigas; Summer; Spat; Pathogen; Disease transmission; Oyster culture; Mortality; Microbial contamination; Oysters.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3343.pdf

In vitro effects of cadmium and mercury on Pacific oyster, Crassostrea gigas (Thunberg), haemocytes
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Thomas Guyon, H; Renault, Tristan.
In the past decades. shellfish culture has developed in a significant way around the world. However, culture areas are Often Subject to recurring anthropic pollution. The recrudescent presence of industrial wastes is a source of heavy metals and results ill pollutant transfer towards the aquatic environment in estuarine areas. Because of their mode of life. bivalves. including Mussels and oysters. are suggested as ideal indicator organisms. The development of techniques allowing the analysis of the effects of Pollutants on bivalve biology may lead to the monitoring of pollutant transfer in estuarine areas. in this context. the effects of cadmium and mercury On defence mechanisms were analysed in Pacific oysters. Crassostrea gigas. Pollutant effects were...
Tipo: Text
Palavras-chave: Toxicity; Cellular activities; Phenoloxidase; Mercury; Cadmium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-691.pdf

Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens
Provedor de dados: 5
Autores: Renault, Tristan; Tchaleu, Gwenaelle; Faury, Nicole; Moreau, Pierrick; Segarra, Amelie; Barbosa-solomieu, Valerie; Lapegue, Sylvie.
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest....
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00172/28306/26582.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 96 RPC R70 - Programme Pathologie - Rapport Scientifique année 1996
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Lipart, Cecile; Thebault, Anne.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00043/15452/12827.pdf

Summer seed mortality of the Pacific oyster, Crassostrea gigas Thunberg grown in Tomales Bay, California, USA : the influence of oyster stock, planting time, pathogens, and environmental stressors
Provedor de dados: 5
Autores: Burge, Colleen; Judah, Linda; Conquest, Loveday; Griffin, Frederick; Cheney, Daniel; Suhrbier, Andrew; Vadopalas, Brent; Olin, Paul G.; Renault, Tristan; Friedman, Carolyn S.
Summer seed mortality (SSM) has occurred yearly in Tomales Bay, California since 1993. SSM has resulted in up to 90% cumulative losses, and has been associated with extreme temperature, phytoplankton blooms, and an oyster herpesvirus. In this study, three stocks of Pacific oysters were planted at three sites in California (Inner Tomales Bay, Outer Tomales Bay, and Bodega Harbor) in October of 2000 (Fall) and April of 2001 (Spring) and monitored for mortality, growth, and health status. In April of 2001, a similar study was conducted in Totten Inlet, WA state using cohorts of oysters planted in California; animals were monitored for mortality and growth. Temperature data were collected at all sites; phytoplankton abundance data were collected  at the...
Tipo: Text
Palavras-chave: California; Tomales Bay; Oyster herpesvirus; Temperature; Mortality; Crassostrea gigas; Pacific oysters.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2976.pdf

Development of a new diagnostic tool (Mini-Array) for ostreid Herpesvrus 1 (OsHV-1) detection in the Pacific oyster Crassostre gigas
Provedor de dados: 5
Autores: Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan.
OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves...
Tipo: Text
Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf

Project ROMEO : E. C. n° FA-S2 9052 - Research objectives : Mortality in European Oysters - Final Report
Provedor de dados: 5
Autores: Renault, Tristan; Arzul, Isabelle.
Crassostrea gigas is increasingly being cultured in a great number of countries from animaIs originally introduced from Japan, and is one of the most exploited bivalve species in the EU (FAO, 1989). Although no serious pathogen was detected previously in Japanese oysters in Europe, mortalities have been observed since the introduction of this bivalve species into the EU. Thus, oyster cultivation may be endangered by the occurrence of epizootics, especially virus diseases, which are considered one of the maj or risks to production. Indeed, mortalities have been described among different species of ostreids and are associated with the presence of viruses belonging to various families. The first description of a virus was reported in adult Eastern oyters,...
Tipo: Text
Palavras-chave: Pathology; Virology; Herpes-like infection; Detection; Diagnosis; Herpesviridae; Oyster; Crassostrea gigas; Mortality.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15536/12915.pdf

Etude des intéractions entre le parasite Bonamia ostreae et son hôte, huître plate Ostrea edulis in vitro
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan.
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in...
Tipo: Text
Palavras-chave: Hémocytes; Ostrea edulis; Bonamia ostreae; Parasite; Huîtres.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3368.pdf

Ostreid herpesvirus type 1 replication and host response in adult Pacific oysters, Crassostrea gigas
Provedor de dados: 5
Autores: Segarra, Amelie; Baillon, Laury; Tourbiez, Delphine; Benabdelmouna, Abdellah; Faury, Nicole; Bourgougnon, Nathalie; Renault, Tristan.
Since 2008, massive mortality outbreaks associated with OsHV-1 detection have been reported in Crassostrea gigas spat and juveniles in several countries. Nevertheless, adult oysters do not demonstrate mortality in the field related to OsHV-1 detection and were thus assumed to be more resistant to viral infection. Determining how virus and adult oyster interact is a major goal in understanding why mortality events are not reported among adult Pacific oysters. Dual transcriptomics of virus-host interactions were explored by real-time PCR in adult oysters after a virus injection. Thirty-nine viral genes and five host genes including MyD88, IFI44, IkB2, IAP and Gly were measured at 0.5, 10, 26, 72 and 144 hours post infection (hpi). No viral RNA among the 39...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00213/32414/30887.pdf

Pesticides and Ostreid Herpesvirus 1 Infection in the Pacific Oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Moreau, Pierrick; Faury, Nicole; Burgeot, Thierry; Renault, Tristan.
Since 2008, mass mortality outbreaks have been reported in all French regions producing Pacific oysters, and in several Member States of the European Union. These mass mortality events of Pacific oysters are related to OsHV-1 infection. They occur during spring and summer periods leaving suspect the quality of the marine environment and the role of seasonal use of pesticides associated with the arrival of freshwater in oyster rearing areas. Pesticides have been also detected in French coastal waters, especially in areas of oyster production. Using PMA real-time PCR we showed that a mixture of 14 pesticides has no effect on the integrity of virus capsids from viral suspension in the conditions tested. A contact of oysters with this pesticide mixture was...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00273/38405/36730.pdf

Ultrastructural study of neoplastic cells in Macoma balthica (Bivalvia) from the Gulf of Gdansk (Poland)
Provedor de dados: 5
Autores: Smolarz, Kasia; Renault, Tristan; Wolowicz, Maciej.
In the Baltic clam Macoma balthica from the Gulf of Gdansk, neoplasia is considered as a serious epizootic linked to bad environmental conditions and high levels of pollutants. Previous research was focused on the diagnosis, prevalence, seasonality and histopathological characteristic of the cancer. This study is focused on electron microscopy analyses describing cell ultrastructure abnormalities related to neoplasia. Examinations using the electron microscopy highlighted changes confined to anatomic ultrastructures, shapes and functions of neoplastic cells. The lobulated appearance of the nucleus, changes in cellular matrix and the occurrence of large granular cells with hyperchromatic nuclei, atypical Golgi structures and deterioration of rough...
Tipo: Text
Palavras-chave: Gulf of Gdansk; Macoma balthica; Neoplasia; Myofibroblast like cells; Cell ultrastructures.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1689.pdf

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Provedor de dados: 5
Autores: Klionsky, Daniel J.; Abdel-aziz, Amal Kamal; Abdelfatah, Sara; Abdellatif, Mahmoud; Abdoli, Asghar; Abel, Steffen; Abeliovich, Hagai; Abildgaard, Marie H.; Abudu, Yakubu Princely; Acevedo-arozena, Abraham; Adamopoulos, Iannis E.; Adeli, Khosrow; Adolph, Timon E.; Adornetto, Annagrazia; Aflaki, Elma; Agam, Galila; Agarwal, Anupam; Aggarwal, Bharat B.; Agnello, Maria; Agostinis, Patrizia; Agrewala, Javed N.; Agrotis, Alexander; Aguilar, Patricia V.; Ahmad, S. Tariq; Ahmed, Zubair M.; Ahumada-castro, Ulises; Aits, Sonja; Aizawa, Shu; Akkoc, Yunus; Akoumianaki, Tonia; Akpinar, Hafize Aysin; Al-abd, Ahmed M.; Al-akra, Lina; Al-gharaibeh, Abeer; Alaoui-jamali, Moulay A.; Alberti, Simon; Alcocer-gómez, Elísabet; Alessandri, Cristiano; Ali, Muhammad; Alim Al-bari, M. Abdul; Aliwaini, Saeb; Alizadeh, Javad; Almacellas, Eugènia; Almasan, Alexandru; Alonso, Alicia; Alonso, Guillermo D.; Altan-bonnet, Nihal; Altieri, Dario C.; Álvarez, Élida M. C.; Alves, Sara; Alves Da Costa, Cristine; Alzaharna, Mazen M.; Amadio, Marialaura; Amantini, Consuelo; Amaral, Cristina; Ambrosio, Susanna; Amer, Amal O.; Ammanathan, Veena; An, Zhenyi; Andersen, Stig U.; Andrabi, Shaida A.; Andrade-silva, Magaiver; Andres, Allen M.; Angelini, Sabrina; Ann, David; Anozie, Uche C.; Ansari, Mohammad Y.; Antas, Pedro; Antebi, Adam; Antón, Zuriñe; Anwar, Tahira; Apetoh, Lionel; Apostolova, Nadezda; Araki, Toshiyuki; Araki, Yasuhiro; Arasaki, Kohei; Araújo, Wagner L.; Araya, Jun; Arden, Catherine; Arévalo, Maria-angeles; Arguelles, Sandro; Arias, Esperanza; Arikkath, Jyothi; Arimoto, Hirokazu; Ariosa, Aileen R.; Armstrong-james, Darius; Arnauné-pelloquin, Laetitia; Aroca, Angeles; Arroyo, Daniela S.; Arsov, Ivica; Artero, Rubén; Asaro, Dalia Maria Lucia; Aschner, Michael; Ashrafizadeh, Milad; Ashur-fabian, Osnat; Atanasov, Atanas G.; Au, Alicia K.; Auberger, Patrick; Auner, Holger W.; Aurelian, Laure; Autelli, Riccardo; Avagliano, Laura; Ávalos, Yenniffer; Aveic, Sanja; Aveleira, Célia Alexandra; Avin-wittenberg, Tamar; Aydin, Yucel; Ayton, Scott; Ayyadevara, Srinivas; Azzopardi, Maria; Baba, Misuzu; Backer, Jonathan M.; Backues, Steven K.; Bae, Dong-hun; Bae, Ok-nam; Bae, Soo Han; Baehrecke, Eric H.; Baek, Ahruem; Baek, Seung-hoon; Baek, Sung Hee; Bagetta, Giacinto; Bagniewska-zadworna, Agnieszka; Bai, Hua; Bai, Jie; Bai, Xiyuan; Bai, Yidong; Bairagi, Nandadulal; Baksi, Shounak; Balbi, Teresa; Baldari, Cosima T.; Balduini, Walter; Ballabio, Andrea; Ballester, Maria; Balazadeh, Salma; Balzan, Rena; Bandopadhyay, Rina; Banerjee, Sreeparna; Banerjee, Sulagna; Bánréti, Ágnes; Bao, Yan; Baptista, Mauricio S.; Baracca, Alessandra; Barbati, Cristiana; Bargiela, Ariadna; Barilà, Daniela; Barlow, Peter G.; Barmada, Sami J.; Barreiro, Esther; Barreto, George E.; Bartek, Jiri; Bartel, Bonnie; Bartolome, Alberto; Barve, Gaurav R.; Basagoudanavar, Suresh H.; Bassham, Diane C.; Bast, Robert C.; Basu, Alakananda; Batoko, Henri; Batten, Isabella; Baulieu, Etienne E.; Baumgarner, Bradley L.; Bayry, Jagadeesh; Beale, Rupert; Beau, Isabelle; Beaumatin, Florian; Bechara, Luiz R.g.; Beck, George R.; Beers, Michael F.; Begun, Jakob; Behrends, Christian; Behrens, Georg M.n.; Bei, Roberto; Bejarano, Eloy; Bel, Shai; Behl, Christian; Belaid, Amine; Belgareh-touzé, Naïma; Bellarosa, Cristina; Belleudi, Francesca; Belló Pérez, Melissa; Bello-morales, Raquel; Beltran, Jackeline Soares De Oliveira; Beltran, Sebastián; Benbrook, Doris Mangiaracina; Bendorius, Mykolas; Benitez, Bruno A.; Benito-cuesta, Irene; Bensalem, Julien; Berchtold, Martin W.; Berezowska, Sabina; Bergamaschi, Daniele; Bergami, Matteo; Bergmann, Andreas; Berliocchi, Laura; Berlioz-torrent, Clarisse; Bernard, Amélie; Berthoux, Lionel; Besirli, Cagri G.; Besteiro, Sebastien; Betin, Virginie M.; Beyaert, Rudi; Bezbradica, Jelena S.; Bhaskar, Kiran; Bhatia-kissova, Ingrid; Bhattacharya, Resham; Bhattacharya, Sujoy; Bhattacharyya, Shalmoli; Bhuiyan, Md. Shenuarin; Bhutia, Sujit Kumar; Bi, Lanrong; Bi, Xiaolin; Biden, Trevor J.; Bijian, Krikor; Billes, Viktor A.; Binart, Nadine; Bincoletto, Claudia; Birgisdottir, Asa B.; Bjorkoy, Geir; Blanco, Gonzalo; Blas-garcia, Ana; Blasiak, Janusz; Blomgran, Robert; Blomgren, Klas; Blum, Janice S.; Boada-romero, Emilio; Boban, Mirta; Boesze-battaglia, Kathleen; Boeuf, Philippe; Boland, Barry; Bomont, Pascale; Bonaldo, Paolo; Bonam, Srinivasa Reddy; Bonfili, Laura; Bonifacino, Juan S.; Boone, Brian A.; Bootman, Martin D.; Bordi, Matteo; Borner, Christoph; Bornhauser, Beat C.; Borthakur, Gautam; Bosch, Jürgen; Bose, Santanu; Botana, Luis M.; Botas, Juan; Boulanger, Chantal M.; Boulton, Michael E.; Bourdenx, Mathieu; Bourgeois, Benjamin; Bourke, Nollaig M.; Bousquet, Guilhem; Boya, Patricia; Bozhkov, Peter V.; Bozi, Luiz H. M.; Bozkurt, Tolga O.; Brackney, Doug E.; Brandts, Christian H.; Braun, Ralf J.; Braus, Gerhard H.; Bravo-sagua, Roberto; Bravo-san Pedro, José M.; Brest, Patrick; Bringer, Marie-agnès; Briones-herrera, Alfredo; Broaddus, V. Courtney; Brodersen, Peter; Brodsky, Jeffrey L.; Brody, Steven L.; Bronson, Paola G.; Bronstein, Jeff M.; Brown, Carolyn N.; Brown, Rhoderick E.; Brum, Patricia C.; Brumell, John H.; Brunetti-pierri, Nicola; Bruno, Daniele; Bryson-richardson, Robert J.; Bucci, Cecilia; Buchrieser, Carmen; Bueno, Marta; Buitrago-molina, Laura Elisa; Buraschi, Simone; Buch, Shilpa; Buchan, J. Ross; Buckingham, Erin M.; Budak, Hikmet; Budini, Mauricio; Bultynck, Geert; Burada, Florin; Burgoyne, Joseph R.; Burón, M. Isabel; Bustos, Victor; Büttner, Sabrina; Butturini, Elena; Byrd, Aaron; Cabas, Isabel; Cabrera-benitez, Sandra; Cadwell, Ken; Cai, Jingjing; Cai, Lu; Cai, Qian; Cairó, Montserrat; Calbet, Jose A.; Caldwell, Guy A.; Caldwell, Kim A.; Call, Jarrod A.; Calvani, Riccardo; Calvo, Ana C.; Calvo-rubio Barrera, Miguel; Camara, Niels Os; Camonis, Jacques H.; Camougrand, Nadine; Campanella, Michelangelo; Campbell, Edward M.; Campbell-valois, François-xavier; Campello, Silvia; Campesi, Ilaria; Campos, Juliane C.; Camuzard, Olivier; Cancino, Jorge; Candido De Almeida, Danilo; Canesi, Laura; Caniggia, Isabella; Canonico, Barbara; Cantí, Carles; Cao, Bin; Caraglia, Michele; Caramés, Beatriz; Carchman, Evie H.; Cardenal-muñoz, Elena; Cardenas, Cesar; Cardenas, Luis; Cardoso, Sandra M.; Carew, Jennifer S.; Carle, Georges F.; Carleton, Gillian; Carloni, Silvia; Carmona-gutierrez, Didac; Carneiro, Leticia A.; Carnevali, Oliana; Carosi, Julian M.; Carra, Serena; Carrier, Alice; Carrier, Lucie; Carroll, Bernadette; Carter, A. Brent; Carvalho, Andreia Neves; Casanova, Magali; Casas, Caty; Casas, Josefina; Cassioli, Chiara; Castillo, Eliseo F.; Castillo, Karen; Castillo-lluva, Sonia; Castoldi, Francesca; Castori, Marco; Castro, Ariel F.; Castro-caldas, Margarida; Castro-hernandez, Javier; Castro-obregon, Susana; Catz, Sergio D.; Cavadas, Claudia; Cavaliere, Federica; Cavallini, Gabriella; Cavinato, Maria; Cayuela, Maria L.; Cebollada Rica, Paula; Cecarini, Valentina; Cecconi, Francesco; Cechowska-pasko, Marzanna; Cenci, Simone; Ceperuelo-mallafré, Victòria; Cerqueira, João J.; Cerutti, Janete M.; Cervia, Davide; Cetintas, Vildan Bozok; Cetrullo, Silvia; Chae, Han-jung; Chagin, Andrei S.; Chai, Chee-yin; Chakrabarti, Gopal; Chakrabarti, Oishee; Chakraborty, Tapas; Chakraborty, Trinad; Chami, Mounia; Chamilos, Georgios; Chan, David W.; Chan, Edmond Y. W.; Chan, Edward D.; Chan, H.y. Edwin; Chan, Helen H.; Chan, Hung; Chan, Matthew T.v.; Chan, Yau Sang; Chandra, Partha K.; Chang, Chih-peng; Chang, Chunmei; Chang, Hao-chun; Chang, Kai; Chao, Jie; Chapman, Tracey; Charlet-berguerand, Nicolas; Chatterjee, Samrat; Chaube, Shail K.; Chaudhary, Anu; Chauhan, Santosh; Chaum, Edward; Checler, Frédéric; Cheetham, Michael E.; Chen, Chang-shi; Chen, Guang-chao; Chen, Jian-fu; Chen, Liam L.; Chen, Leilei; Chen, Lin; Chen, Mingliang; Chen, Mu-kuan; Chen, Ning; Chen, Quan; Chen, Ruey-hwa; Chen, Shi; Chen, Wei; Chen, Weiqiang; Chen, Xin-ming; Chen, Xiong-wen; Chen, Xu; Chen, Yan; Chen, Ye-guang; Chen, Yingyu; Chen, Yongqiang; Chen, Yu-jen; Chen, Yue-qin; Chen, Zhefan Stephen; Chen, Zhi; Chen, Zhi-hua; Chen, Zhijian J.; Chen, Zhixiang; Cheng, Hanhua; Cheng, Jun; Cheng, Shi-yuan; Cheng, Wei; Cheng, Xiaodong; Cheng, Xiu-tang; Cheng, Yiyun; Cheng, Zhiyong; Chen, Zhong; Cheong, Heesun; Cheong, Jit Kong; Chernyak, Boris V.; Cherry, Sara; Cheung, Chi Fai Randy; Cheung, Chun Hei Antonio; Cheung, King-ho; Chevet, Eric; Chi, Richard J.; Chiang, Alan Kwok Shing; Chiaradonna, Ferdinando; Chiarelli, Roberto; Chiariello, Mario; Chica, Nathalia; Chiocca, Susanna; Chiong, Mario; Chiou, Shih-hwa; Chiramel, Abhilash I.; Chiurchiù, Valerio; Cho, Dong-hyung; Choe, Seong-kyu; Choi, Augustine M.k.; Choi, Mary E.; Choudhury, Kamalika Roy; Chow, Norman S.; Chu, Charleen T.; Chua, Jason P.; Chua, John Jia En; Chung, Hyewon; Chung, Kin Pan; Chung, Seockhoon; Chung, So-hyang; Chung, Yuen-li; Cianfanelli, Valentina; Ciechomska, Iwona A.; Cifuentes, Mariana; Cinque, Laura; Cirak, Sebahattin; Cirone, Mara; Clague, Michael J.; Clarke, Robert; Clementi, Emilio; Coccia, Eliana M.; Codogno, Patrice; Cohen, Ehud; Cohen, Mickael M.; Colasanti, Tania; Colasuonno, Fiorella; Colbert, Robert A.; Colell, Anna; Čolić, Miodrag; Coll, Nuria S.; Collins, Mark O.; Colombo, María I.; Colón-ramos, Daniel A.; Combaret, Lydie; Comincini, Sergio; Cominetti, Márcia R.; Consiglio, Antonella; Conte, Andrea; Conti, Fabrizio; Contu, Viorica Raluca; Cookson, Mark R.; Coombs, Kevin M.; Coppens, Isabelle; Corasaniti, Maria Tiziana; Corkery, Dale P.; Cordes, Nils; Cortese, Katia; Costa, Maria Do Carmo; Costantino, Sarah; Costelli, Paola; Coto-montes, Ana; Crack, Peter J.; Crespo, Jose L.; Criollo, Alfredo; Crippa, Valeria; Cristofani, Riccardo; Csizmadia, Tamas; Cuadrado, Antonio; Cui, Bing; Cui, Jun; Cui, Yixian; Cui, Yong; Culetto, Emmanuel; Cumino, Andrea C.; Cybulsky, Andrey V.; Czaja, Mark J.; Czuczwar, Stanislaw J.; D’adamo, Stefania; D’amelio, Marcello; D’arcangelo, Daniela; D’lugos, Andrew C.; D’orazi, Gabriella; Da Silva, James A.; Dafsari, Hormos Salimi; Dagda, Ruben K.; Dagdas, Yasin; Daglia, Maria; Dai, Xiaoxia; Dai, Yun; Dai, Yuyuan; Dal Col, Jessica; Dalhaimer, Paul; Dalla Valle, Luisa; Dallenga, Tobias; Dalmasso, Guillaume; Damme, Markus; Dando, Ilaria; Dantuma, Nico P.; Darling, April L.; Das, Hiranmoy; Dasarathy, Srinivasan; Dasari, Santosh K.; Dash, Srikanta; Daumke, Oliver; Dauphinee, Adrian N.; Davies, Jeffrey S.; Dávila, Valeria A.; Davis, Roger J.; Davis, Tanja; Dayalan Naidu, Sharadha; De Amicis, Francesca; De Bosscher, Karolien; De Felice, Francesca; De Franceschi, Lucia; De Leonibus, Chiara; De Mattos Barbosa, Mayara G.; De Meyer, Guido R.y.; De Milito, Angelo; De Nunzio, Cosimo; De Palma, Clara; De Santi, Mauro; De Virgilio, Claudio; De Zio, Daniela; Debnath, Jayanta; Debosch, Brian J.; Decuypere, Jean-paul; Deehan, Mark A.; Deflorian, Gianluca; Degregori, James; Dehay, Benjamin; Del Rio, Gabriel; Delaney, Joe R.; Delbridge, Lea M. D.; Delorme-axford, Elizabeth; Delpino, M. Victoria; Demarchi, Francesca; Dembitz, Vilma; Demers, Nicholas D.; Deng, Hongbin; Deng, Zhiqiang; Dengjel, Joern; Dent, Paul; Denton, Donna; Depamphilis, Melvin L.; Der, Channing J.; Deretic, Vojo; Descoteaux, Albert; Devis, Laura; Devkota, Sushil; Devuyst, Olivier; Dewson, Grant; Dharmasivam, Mahendiran; Dhiman, Rohan; Di Bernardo, Diego; Di Cristina, Manlio; Di Domenico, Fabio; Di Fazio, Pietro; Di Fonzo, Alessio; Di Guardo, Giovanni; Di Guglielmo, Gianni M.; Di Leo, Luca; Di Malta, Chiara; Di Nardo, Alessia; Di Rienzo, Martina; Di Sano, Federica; Diallinas, George; Diao, Jiajie; Diaz-araya, Guillermo; Díaz-laviada, Inés; Dickinson, Jared M.; Diederich, Marc; Dieudé, Mélanie; Dikic, Ivan; Ding, Shiping; Ding, Wen-xing; Dini, Luciana; Dinić, Jelena; Dinic, Miroslav; Dinkova-kostova, Albena T.; Dionne, Marc S.; Distler, Jörg H.w.; Diwan, Abhinav; Dixon, Ian M.c.; Djavaheri-mergny, Mojgan; Dobrinski, Ina; Dobrovinskaya, Oxana; Dobrowolski, Radek; Dobson, Renwick C.j.; Đokić, Jelena; Dokmeci Emre, Serap; Donadelli, Massimo; Dong, Bo; Dong, Xiaonan; Dong, Zhiwu; Dorn Ii, Gerald W.; Dotsch, Volker; Dou, Huan; Dou, Juan; Dowaidar, Moataz; Dridi, Sami; Drucker, Liat; Du, Ailian; Du, Caigan; Du, Guangwei; Du, Hai-ning; Du, Li-lin; Du Toit, André; Duan, Shao-bin; Duan, Xiaoqiong; Duarte, Sónia P.; Dubrovska, Anna; Dunlop, Elaine A.; Dupont, Nicolas; Durán, Raúl V.; Dwarakanath, Bilikere S.; Dyshlovoy, Sergey A.; Ebrahimi-fakhari, Darius; Eckhart, Leopold; Edelstein, Charles L.; Efferth, Thomas; Eftekharpour, Eftekhar; Eichinger, Ludwig; Eid, Nabil; Eisenberg, Tobias; Eissa, N. Tony; Eissa, Sanaa; Ejarque, Miriam; El Andaloussi, Abdeljabar; El-hage, Nazira; El-naggar, Shahenda; Eleuteri, Anna Maria; El-shafey, Eman S.; Elgendy, Mohamed; Eliopoulos, Aristides G.; Elizalde, María M.; Elks, Philip M.; Elsasser, Hans-peter; Elsherbiny, Eslam S.; Emerling, Brooke M.; Emre, N. C. Tolga; Eng, Christina H.; Engedal, Nikolai; Engelbrecht, Anna-mart; Engelsen, Agnete S.t.; Enserink, Jorrit M.; Escalante, Ricardo; Esclatine, Audrey; Escobar-henriques, Mafalda; Eskelinen, Eeva-liisa; Espert, Lucile; Eusebio, Makandjou-ola; Fabrias, Gemma; Fabrizi, Cinzia; Facchiano, Antonio; Facchiano, Francesco; Fadeel, Bengt; Fader, Claudio; Faesen, Alex C.; Fairlie, W. Douglas; Falcó, Alberto; Falkenburger, Bjorn H.; Fan, Daping; Fan, Jie; Fan, Yanbo; Fang, Evandro F.; Fang, Yanshan; Fang, Yognqi; Fanto, Manolis; Farfel-becker, Tamar; Faure, Mathias; Fazeli, Gholamreza; Fedele, Anthony O.; Feldman, Arthur M.; Feng, Du; Feng, Jiachun; Feng, Lifeng; Feng, Yibin; Feng, Yuchen; Feng, Wei; Fenz Araujo, Thais; Ferguson, Thomas A.; Fernández, Álvaro F.; Fernandez-checa, Jose C.; Fernández-veledo, Sonia; Fernie, Alisdair R.; Ferrante, Anthony W.; Ferraresi, Alessandra; Ferrari, Merari F.; Ferreira, Julio C.b.; Ferro-novick, Susan; Figueras, Antonio; Filadi, Riccardo; Filigheddu, Nicoletta; Filippi-chiela, Eduardo; Filomeni, Giuseppe; Fimia, Gian Maria; Fineschi, Vittorio; Finetti, Francesca; Finkbeiner, Steven; Fisher, Edward A.; Fisher, Paul B.; Flamigni, Flavio; Fliesler, Steven J.; Flo, Trude H.; Florance, Ida; Florey, Oliver; Florio, Tullio; Fodor, Erika; Follo, Carlo; Fon, Edward A.; Forlino, Antonella; Fornai, Francesco; Fortini, Paola; Fracassi, Anna; Fraldi, Alessandro; Franco, Brunella; Franco, Rodrigo; Franconi, Flavia; Frankel, Lisa B.; Friedman, Scott L.; Fröhlich, Leopold F.; Frühbeck, Gema; Fuentes, Jose M.; Fujiki, Yukio; Fujita, Naonobu; Fujiwara, Yuuki; Fukuda, Mitsunori; Fulda, Simone; Furic, Luc; Furuya, Norihiko; Fusco, Carmela; Gack, Michaela U.; Gaffke, Lidia; Galadari, Sehamuddin; Galasso, Alessia; Galindo, Maria F.; Gallolu Kankanamalage, Sachith; Galluzzi, Lorenzo; Galy, Vincent; Gammoh, Noor; Gan, Boyi; Ganley, Ian G.; Gao, Feng; Gao, Hui; Gao, Minghui; Gao, Ping; Gao, Shou-jiang; Gao, Wentao; Gao, Xiaobo; Garcera, Ana; Garcia, Maria Noé; Garcia, Verónica E.; García-del Portillo, Francisco; Garcia-escudero, Vega; Garcia-garcia, Aracely; Garcia-macia, Marina; García-moreno, Diana; Garcia-ruiz, Carmen; García-sanz, Patricia; Garg, Abhishek D.; Gargini, Ricardo; Garofalo, Tina; Garry, Robert F.; Gassen, Nils C.; Gatica, Damian; Ge, Liang; Ge, Wanzhong; Geiss-friedlander, Ruth; Gelfi, Cecilia; Genschik, Pascal; Gentle, Ian E.; Gerbino, Valeria; Gerhardt, Christoph; Germain, Kyla; Germain, Marc; Gewirtz, David A.; Ghasemipour Afshar, Elham; Ghavami, Saeid; Ghigo, Alessandra; Ghosh, Manosij; Giamas, Georgios; Giampietri, Claudia; Giatromanolaki, Alexandra; Gibson, Gary E.; Gibson, Spencer B.; Ginet, Vanessa; Giniger, Edward; Giorgi, Carlotta; Girao, Henrique; Girardin, Stephen E.; Giridharan, Mridhula; Giuliano, Sandy; Giulivi, Cecilia; Giuriato, Sylvie; Giustiniani, Julien; Gluschko, Alexander; Goder, Veit; Goginashvili, Alexander; Golab, Jakub; Goldstone, David C.; Golebiewska, Anna; Gomes, Luciana R.; Gomez, Rodrigo; Gómez-sánchez, Rubén; Gomez-puerto, Maria Catalina; Gomez-sintes, Raquel; Gong, Qingqiu; Goni, Felix M.; González-gallego, Javier; Gonzalez-hernandez, Tomas; Gonzalez-polo, Rosa A.; Gonzalez-reyes, Jose A.; González-rodríguez, Patricia; Goping, Ing Swie; Gorbatyuk, Marina S.; Gorbunov, Nikolai V.; Görgülü, Kıvanç; Gorojod, Roxana M.; Gorski, Sharon M.; Goruppi, Sandro; Gotor, Cecilia; Gottlieb, Roberta A.; Gozes, Illana; Gozuacik, Devrim; Graef, Martin; Gräler, Markus H.; Granatiero, Veronica; Grasso, Daniel; Gray, Joshua P.; Green, Douglas R.; Greenhough, Alexander; Gregory, Stephen L.; Griffin, Edward F.; Grinstaff, Mark W.; Gros, Frederic; Grose, Charles; Gross, Angelina S.; Gruber, Florian; Grumati, Paolo; Grune, Tilman; Gu, Xueyan; Guan, Jun-lin; Guardia, Carlos M.; Guda, Kishore; Guerra, Flora; Guerri, Consuelo; Guha, Prasun; Guillén, Carlos; Gujar, Shashi; Gukovskaya, Anna; Gukovsky, Ilya; Gunst, Jan; Günther, Andreas; Guntur, Anyonya R.; Guo, Chuanyong; Guo, Chun; Guo, Hongqing; Guo, Lian-wang; Guo, Ming; Gupta, Pawan; Gupta, Shashi Kumar; Gupta, Swapnil; Gupta, Veer Bala; Gupta, Vivek; Gustafsson, Asa B.; Gutterman, David D.; H.b., Ranjitha; Haapasalo, Annakaisa; Haber, James E.; Hać, Aleksandra; Hadano, Shinji; Hafrén, Anders J.; Haidar, Mansour; Hall, Belinda S.; Halldén, Gunnel; Hamacher-brady, Anne; Hamann, Andrea; Hamasaki, Maho; Han, Weidong; Hansen, Malene; Hanson, Phyllis I. ; Hao, Zijian; Harada, Masaru; Harhaji-trajkovic, Ljubica; Hariharan, Nirmala; Haroon, Nigil; Harris, James; Hasegawa, Takafumi; Hasima Nagoor, Noor; Haspel, Jeffrey A.; Haucke, Volker; Hawkins, Wayne D.; Hay, Bruce A.; Haynes, Cole M.; Hayrabedyan, Soren B.; Hays, Thomas S.; He, Congcong; He, Qin; He, Rong-rong; He, You-wen; He, Yu-ying; Heakal, Yasser; Heberle, Alexander M.; Hejtmancik, J. Fielding; Helgason, Gudmundur Vignir ; Henkel, Vanessa; Herb, Marc; Hergovich, Alexander; Herman-antosiewicz, Anna; Hernández, Agustín; Hernandez, Carlos; Hernandez-diaz, Sergio; Hernandez-gea, Virginia; Herpin, Amaury; Herreros, Judit; Hervás, Javier H.; Hesselson, Daniel; Hetz, Claudio; Heussler, Volker T.; Higuchi, Yujiro; Hilfiker, Sabine; Hill, Joseph A.; Hlavacek, William S.; Ho, Emmanuel A.; Ho, Idy H.t.; Ho, Philip Wing-lok; Ho, Shu-leong; Ho, Wan Yun; Hobbs, G. Aaron; Hochstrasser, Mark; Hoet, Peter H.m.; Hofius, Daniel; Hofman, Paul; Höhn, Annika; Holmberg, Carina I.; Hombrebueno, Jose R.; Yi-ren Hong, Chang-won Hong,; Hooper, Lora V.; Hoppe, Thorsten; Horos, Rastislav; Hoshida, Yujin; Hsin, I-lun; Hsu, Hsin-yun; Hu, Bing; Hu, Dong; Hu, Li-fang; Hu, Ming Chang; Hu, Ronggui; Hu, Wei; Hu, Yu-chen; Hu, Zhuo-wei; Hua, Fang; Hua, Jinlian; Hua, Yingqi; Huan, Chongmin; Huang, Canhua; Huang, Chuanshu; Huang, Chuanxin; Huang, Chunling; Huang, Haishan; Huang, Kun; Huang, Michael L.h.; Huang, Rui; Huang, Shan; Huang, Tianzhi; Huang, Xing; Huang, Yuxiang Jack; Huber, Tobias B.; Hubert, Virginie; Hubner, Christian A.; Hughes, Stephanie M.; Hughes, William E.; Humbert, Magali; Hummer, Gerhard; Hurley, James H.; Hussain, Sabah; Hussain, Salik; Hussey, Patrick J.; Hutabarat, Martina; Hwang, Hui-yun; Hwang, Seungmin; Ieni, Antonio; Ikeda, Fumiyo; Imagawa, Yusuke; Imai, Yuzuru; Imbriano, Carol; Imoto, Masaya; Inman, Denise M.; Inoki, Ken; Iovanna, Juan; Iozzo, Renato V.; Ippolito, Giuseppe; Irazoqui, Javier E.; Iribarren, Pablo; Ishaq, Mohd; Ishikawa, Makoto; Ishimwe, Nestor; Isidoro, Ciro; Ismail, Nahed; Issazadeh-navikas, Shohreh; Itakura, Eisuke; Ito, Daisuke; Ivankovic, Davor; Ivanova, Saška; Iyer, Anand Krishnan V.; Izquierdo, José M.; Izumi, Masanori; Jäättelä, Marja; Jabir, Majid Sakhi; Jackson, William T.; Jacobo-herrera, Nadia; Jacomin, Anne-claire; Jacquin, Elise; Jadiya, Pooja; Jaeschke, Hartmut; Jagannath, Chinnaswamy; Jakobi, Arjen J.; Jakobsson, Johan; Janji, Bassam; Jansen-dürr, Pidder; Jansson, Patric J.; Jantsch, Jonathan; Januszewski, Sławomir; Jassey, Alagie; Jean, Steve; Jeltsch-david, Hélène; Jendelova, Pavla; Jenny, Andreas; Jensen, Thomas E.; Jessen, Niels; Jewell, Jenna L.; Ji, Jing; Jia, Lijun; Jia, Rui; Jiang, Liwen; Jiang, Qing; Jiang, Richeng; Jiang, Teng; Jiang, Xuejun; Jiang, Yu; Jimenez-sanchez, Maria; Jin, Eun-jung; Jin, Fengyan; Jin, Hongchuan; Jin, Li; Jin, Luqi; Jin, Meiyan; Jin, Si; Jo, Eun-kyeong; Joffre, Carine; Johansen, Terje; Johnson, Gail V.w.; Johnston, Simon A.; Jokitalo, Eija; Jolly, Mohit Kumar; Joosten, Leo A.b.; Jordan, Joaquin; Joseph, Bertrand; Ju, Dianwen; Ju, Jeong-sun; Ju, Jingfang; Juárez, Esmeralda; Judith, Delphine; Juhász, Gábor; Jun, Youngsoo; Jung, Chang Hwa; Jung, Sung-chul; Jung, Yong Keun; Jungbluth, Heinz; Jungverdorben, Johannes; Just, Steffen; Kaarniranta, Kai; Kaasik, Allen; Kabuta, Tomohiro; Kaganovich, Daniel; Kahana, Alon; Kain, Renate; Kajimura, Shinjo; Kalamvoki, Maria; Kalia, Manjula; Kalinowski, Danuta S.; Kaludercic, Nina; Kalvari, Ioanna; Kaminska, Joanna; Kaminskyy, Vitaliy O.; Kanamori, Hiromitsu; Kanasaki, Keizo; Kang, Chanhee; Kang, Rui; Kang, Sang Sun; Kaniyappan, Senthilvelrajan; Kanki, Tomotake; Kanneganti, Thirumala-devi; Kanthasamy, Anumantha G.; Kanthasamy, Arthi; Kantorow, Marc; Kapuy, Orsolya; Karamouzis, Michalis V.; Karim, Md. Razaul; Karmakar, Parimal; Katare, Rajesh G.; Kato, Masaru; Kaufmann, Stefan H.e.; Kauppinen, Anu; Kaushal, Gur P.; Kaushik, Susmita; Kawasaki, Kiyoshi; Kazan, Kemal; Ke, Po-yuan; Keating, Damien J.; Keber, Ursula; Kehrl, John H.; Keller, Kate E.; Keller, Christian W.; Kemper, Jongsook Kim; Kenific, Candia M.; Kepp, Oliver; Kermorgant, Stephanie; Kern, Andreas; Ketteler, Robin; Keulers, Tom G.; Khalfin, Boris; Khalil, Hany; Khambu, Bilon; Khan, Shahid Y.; Khandelwal, Vinoth Kumar Megraj; Khandia, Rekha; Kho, Widuri; Khobrekar, Noopur V.; Khuansuwan, Sataree; Khundadze, Mukhran; Killackey, Samuel A.; Kim, Dasol; Kim, Deok Ryong; Kim, Do-hyung; Kim, Dong-eun; Kim, Eun Young; Kim, Eun-kyoung; Kim, Hak-rim; Kim, Hee-sik; Hyung-ryong Kim,; Kim, Jeong Hun; Kim, Jin Kyung; Kim, Jin-hoi; Kim, Joungmok; Kim, Ju Hwan; Kim, Keun Il; Kim, Peter K.; Kim, Seong-jun; Kimball, Scot R.; Kimchi, Adi; Kimmelman, Alec C.; Kimura, Tomonori; King, Matthew A.; Kinghorn, Kerri J.; Kinsey, Conan G.; Kirkin, Vladimir; Kirshenbaum, Lorrie A.; Kiselev, Sergey L.; Kishi, Shuji; Kitamoto, Katsuhiko; Kitaoka, Yasushi; Kitazato, Kaio; Kitsis, Richard N.; Kittler, Josef T.; Kjaerulff, Ole; Klein, Peter S.; Klopstock, Thomas; Klucken, Jochen; Knævelsrud, Helene; Knorr, Roland L.; Ko, Ben C.b.; Ko, Fred; Ko, Jiunn-liang; Kobayashi, Hotaka; Kobayashi, Satoru; Koch, Ina; Koch, Jan C.; Koenig, Ulrich; Kögel, Donat; Koh, Young Ho; Koike, Masato; Kohlwein, Sepp D.; Kocaturk, Nur M.; Komatsu, Masaaki; König, Jeannette; Kono, Toru; Kopp, Benjamin T.; Korcsmaros, Tamas; Korkmaz, Gözde; Korolchuk, Viktor I.; Korsnes, Mónica Suárez; Koskela, Ali; Kota, Janaiah; Kotake, Yaichiro; Kotler, Monica L.; Kou, Yanjun; Koukourakis, Michael I.; Koustas, Evangelos; Kovacs, Attila L.; Kovács, Tibor; Koya, Daisuke; Kozako, Tomohiro; Kraft, Claudine; Krainc, Dimitri; Krämer, Helmut; Krasnodembskaya, Anna D.; Kretz-remy, Carole; Kroemer, Guido; Ktistakis, Nicholas T.; Kuchitsu, Kazuyuki; Kuenen, Sabine; Kuerschner, Lars; Kukar, Thomas; Kumar, Ajay; Kumar, Ashok; Kumar, Deepak; Kumar, Dhiraj; Kumar, Sharad; Kume, Shinji; Kumsta, Caroline; Kundu, Chanakya N.; Kundu, Mondira; Kunnumakkara, Ajaikumar B.; Kurgan, Lukasz; Kutateladze, Tatiana G.; Kutlu, Ozlem; Kwak, Seongae; Kwon, Ho Jeong; Kwon, Taeg Kyu; Kwon, Yong Tae; Kyrmizi, Irene; La Spada, Albert; Labonté, Patrick; Ladoire, Sylvain; Laface, Ilaria; Lafont, Frank; Lagace, Diane C.; Lahiri, Vikramjit; Lai, Zhibing; Laird, Angela S.; Lakkaraju, Aparna; Lamark, Trond; Lan, Sheng-hui; Landajuela, Ane; Lane, Darius J. R.; Lane, Jon D.; Lang, Charles H.; Lange, Carsten; Langel, Ülo; Langer, Rupert; Lapaquette, Pierre; Laporte, Jocelyn; Larusso, Nicholas F.; Lastres-becker, Isabel; Lau, Wilson Chun Yu; Laurie, Gordon W.; Lavandero, Sergio; Law, Betty Yuen Kwan; Law, Helen Ka-wai; Layfield, Rob; Le, Weidong; Le Stunff, Herve; Leary, Alexandre Y.; Lebrun, Jean-jacques; Leck, Lionel Y.w.; Leduc-gaudet, Jean-philippe; Lee, Changwook; Lee, Chung-pei; Lee, Da-hye; Lee, Edward B.; Lee, Erinna F.; Lee, Gyun Min; Lee, He-jin; Lee, Heung Kyu; Lee, Jae Man; Lee, Jason S.; Lee, Jin-a; Lee, Joo-yong; Lee, Jun Hee; Lee, Michael; Lee, Min Goo; Lee, Min Jae; Lee, Myung-shik; Lee, Sang Yoon; Lee, Seung-jae; Lee, Stella Y.; Lee, Sung Bae; Lee, Won Hee; Lee, Ying-ray; Lee, Yong-ho; Lee, Youngil; Lefebvre, Christophe; Legouis, Renaud; Lei, Yu L.; Lei, Yuchen; Leikin, Sergey; Leitinger, Gerd; Lemus, Leticia; Leng, Shuilong; Lenoir, Olivia; Lenz, Guido; Lenz, Heinz Josef; Lenzi, Paola; León, Yolanda; Leopoldino, Andréia M.; Leschczyk, Christoph; Leskelä, Stina; Letellier, Elisabeth; Leung, Chi-ting; Leung, Po Sing; Leventhal, Jeremy S.; Levine, Beth; Lewis, Patrick A.; Ley, Klaus; Li, Bin; Li, Da-qiang; Li, Jianming; Li, Jing; Li, Jiong; Li, Ke; Li, Liwu; Li, Mei; Li, Min; Li, Min; Li, Ming; Li, Mingchuan; Li, Pin-lan; Li, Ming-qing; Li, Qing; Li, Sheng; Li, Tiangang; Li, Wei; Li, Wenming; Li, Xue; Li, Yi-ping; Li, Yuan; Li, Zhiqiang; Li, Zhiyong; Li, Zhiyuan; Lian, Jiqin; Liang, Chengyu; Liang, Qiangrong; Liang, Weicheng; Liang, Yongheng; Liang, Yongtian; Liao, Guanghong; Liao, Lujian; Liao, Mingzhi; Liao, Yung-feng; Librizzi, Mariangela; Lie, Pearl P. Y.; Lilly, Mary A.; Lim, Hyunjung J.; Lima, Thania R.r.; Limana, Federica; Lin, Chao; Lin, Chih-wen; Lin, Dar-shong; Lin, Fu-cheng; Lin, Jiandie D.; Lin, Kurt M.; Lin, Kwang-huei; Lin, Liang-tzung; Lin, Pei-hui; Lin, Qiong; Lin, Shaofeng; Lin, Su-ju; Lin, Wenyu; Lin, Xueying; Lin, Yao-xin; Lin, Yee-shin; Linden, Rafael; Lindner, Paula; Ling, Shuo-chien; Lingor, Paul; Linnemann, Amelia K.; Liou, Yih-cherng; Lipinski, Marta M.; Lipovšek, Saška; Lira, Vitor A.; Lisiak, Natalia; Liton, Paloma B.; Liu, Chao; Liu, Ching-hsuan; Liu, Chun-feng; Liu, Cui Hua; Liu, Fang; Liu, Hao; Liu, Hsiao-sheng; Liu, Hua-feng; Liu, Huifang; Liu, Jia; Liu, Jing; Liu, Julia; Liu, Leyuan; Liu, Longhua; Liu, Meilian; Liu, Qin; Liu, Wei; Liu, Wende; Liu, Xiao-hong; Liu, Xiaodong; Liu, Xingguo; Liu, Xu; Liu, Xuedong; Liu, Yanfen; Liu, Yang; Liu, Yang; Liu, Yueyang; Liu, Yule; Livingston, J. Andrew; Lizard, Gerard; Lizcano, Jose M.; Ljubojevic-holzer, Senka; Lleonart, Matilde E.; Llobet-navàs, David; Llorente, Alicia; Lo, Chih Hung; Lobato-márquez, Damián; Long, Qi; Long, Yun Chau; Loos, Ben; Loos, Julia A.; López, Manuela G.; López-doménech, Guillermo; López-guerrero, José Antonio; López-jiménez, Ana T.; López-pérez, Óscar; López-valero, Israel; Lorenowicz, Magdalena J.; Lorente, Mar; Lorincz, Peter; Lossi, Laura; Lotersztajn, Sophie; Lovat, Penny E.; Lovell, Jonathan F.; Lovy, Alenka; Lőw, Péter; Lu, Guang; Lu, Haocheng; Lu, Jia-hong; Lu, Jin-jian; Lu, Mengji; Lu, Shuyan; Luciani, Alessandro; Lucocq, John M.; Ludovico, Paula; Luftig, Micah A.; Luhr, Morten; Luis-ravelo, Diego; Lum, Julian J.; Luna-dulcey, Liany; Lund, Anders H.; Lund, Viktor K.; Lünemann, Jan D.; Lüningschrör, Patrick; Luo, Honglin; Luo, Rongcan; Luo, Shouqing; Luo, Zhi; Luparello, Claudio; Lüscher, Bernhard; Luu, Luan; Lyakhovich, Alex; Lyamzaev, Konstantin G.; Lystad, Alf Håkon; Lytvynchuk, Lyubomyr; Ma, Alvin C.; Ma, Changle; Ma, Mengxiao; Ma, Ning-fang; Ma, Quan-hong; Ma, Xinliang; Ma, Yueyun; Ma, Zhenyi; Macdougald, Ormond A.; Macian, Fernando; Macintosh, Gustavo C.; Mackeigan, Jeffrey P.; Macleod, Kay F.; Maday, Sandra; Madeo, Frank; Madesh, Muniswamy; Madl, Tobias; Madrigal-matute, Julio; Maeda, Akiko; Maejima, Yasuhiro; Magarinos, Marta; Mahavadi, Poornima; Maiani, Emiliano; Maiese, Kenneth; Maiti, Panchanan; Maiuri, Maria Chiara; Majello, Barbara; Major, Michael B.; Makareeva, Elena; Malik, Fayaz; Mallilankaraman, Karthik; Malorni, Walter; Maloyan, Alina; Mammadova, Najiba; Man, Gene Chi Wai; Manai, Federico; Mancias, Joseph D.; Mandelkow, Eva-maria; Mandell, Michael A.; Manfredi, Angelo A.; Manjili, Masoud H.; Manjithaya, Ravi; Manque, Patricio; Manshian, Bella B.; Manzano, Raquel; Manzoni, Claudia; Mao, Kai; Marchese, Cinzia; Marchetti, Sandrine; Marconi, Anna Maria; Marcucci, Fabrizio; Mardente, Stefania; Mareninova, Olga A.; Margeta, Marta; Mari, Muriel; Marinelli, Sara; Marinelli, Oliviero; Mariño, Guillermo; Mariotto, Sofia; Marshall, Richard S.; Marten, Mark R.; Martens, Sascha; Martin, Alexandre P.j.; Martin, Katie R.; Martin, Sara; Martin, Shaun; Martín-segura, Adrián; Martín-acebes, Miguel A.; Martin-burriel, Inmaculada; Martin-rincon, Marcos; Martin-sanz, Paloma; Martina, José A.; Martinet, Wim; Martinez, Aitor; Martinez, Ana; Martinez, Jennifer; Martinez Velazquez, Moises; Martinez-lopez, Nuria; Martinez-vicente, Marta; Martins, Daniel O.; Martins, Joilson O.; Martins, Waleska K.; Martins-marques, Tania; Marzetti, Emanuele; Masaldan, Shashank; Masclaux-daubresse, Celine; Mashek, Douglas G.; Massa, Valentina; Massieu, Lourdes; Masson, Glenn R.; Masuelli, Laura; Masyuk, Anatoliy I.; Masyuk, Tetyana V.; Matarrese, Paola; Matheu, Ander; Matoba, Satoaki; Matsuzaki, Sachiko; Mattar, Pamela; Matte, Alessandro; Mattoscio, Domenico; Mauriz, José L.; Mauthe, Mario; Mauvezin, Caroline; Maverakis, Emanual; Maycotte, Paola; Mayer, Johanna; Mazzoccoli, Gianluigi; Mazzoni, Cristina; Mazzulli, Joseph R.; Mccarty, Nami; Mcdonald, Christine; Mcgill, Mitchell R.; Mckenna, Sharon L.; Mclaughlin, Bethann; Mcloughlin, Fionn; Mcniven, Mark A.; Mcwilliams, Thomas G.; Mechta-grigoriou, Fatima; Medeiros, Tania Catarina; Medina, Diego L.; Megeney, Lynn A.; Megyeri, Klara; Mehrpour, Maryam; Mehta, Jawahar L.; Meijer, Alfred J.; Meijer, Annemarie H.; Mejlvang, Jakob; Meléndez, Alicia; Melk, Annette; Memisoglu, Gonen; Mendes, Alexandrina F.; Meng, Delong; Meng, Fei; Meng, Tian; Menna-barreto, Rubem; Menon, Manoj B.; Mercer, Carol; Mercier, Anne E.; Mergny, Jean-louis; Merighi, Adalberto; Merkley, Seth D.; Merla, Giuseppe; Meske, Volker; Mestre, Ana Cecilia; Metur, Shree Padma; Meyer, Christian; Meyer, Hemmo; Mi, Wenyi; Mialet-perez, Jeanne; Miao, Junying; Micale, Lucia; Miki, Yasuo; Milan, Enrico; Milczarek, Małgorzata; Miller, Dana L.; Miller, Samuel I.; Miller, Silke; Millward, Steven W.; Milosevic, Ira; Minina, Elena A.; Mirzaei, Hamed; Mirzaei, Hamid Reza; Mirzaei, Mehdi; Mishra, Amit; Mishra, Nandita; Mishra, Paras Kumar; Misirkic Marjanovic, Maja; Misasi, Roberta; Misra, Amit; Misso, Gabriella; Mitchell, Claire; Mitou, Geraldine; Miura, Tetsuji; Miyamoto, Shigeki; Miyazaki, Makoto; Miyazaki, Mitsunori; Miyazaki, Taiga; Miyazawa, Keisuke; Mizushima, Noboru; Mogensen, Trine H.; Mograbi, Baharia; Mohammadinejad, Reza; Mohamud, Yasir; Mohanty, Abhishek; Mohapatra, Sipra; Möhlmann, Torsten; Mohmmed, Asif; Moles, Anna; Moley, Kelle H.; Molinari, Maurizio; Mollace, Vincenzo; Møller, Andreas Buch; Mollereau, Bertrand; Mollinedo, Faustino; Montagna, Costanza; Monteiro, Mervyn J.; Montella, Andrea; Montes, L. Ruth; Montico, Barbara; Mony, Vinod K.; Monzio Compagnoni, Giacomo; Moore, Michael N.; Moosavi, Mohammad A.; Mora, Ana L.; Mora, Marina; Morales-alamo, David; Moratalla, Rosario; Moreira, Paula I.; Morelli, Elena; Moreno, Sandra; Moreno-blas, Daniel; Moresi, Viviana; Morga, Benjamin; Morgan, Alwena H.; Morin, Fabrice; Morishita, Hideaki; Moritz, Orson L.; Moriyama, Mariko; Moriyasu, Yuji; Morleo, Manuela; Morselli, Eugenia; Moruno-manchon, Jose F.; Moscat, Jorge; Mostowy, Serge; Motori, Elisa; Moura, Andrea Felinto; Moustaid-moussa, Naima; Mrakovcic, Maria; Muciño-hernández, Gabriel; Mukherjee, Anupam; Mukhopadhyay, Subhadip; Mulcahy Levy, Jean M.; Mulero, Victoriano; Muller, Sylviane; Münch, Christian; Munjal, Ashok; Munoz-canoves, Pura; Muñoz-galdeano, Teresa; Münz, Christian; Murakawa, Tomokazu; Muratori, Claudia; Murphy, Brona M.; Murphy, J. Patrick; Murthy, Aditya; Myöhänen, Timo T.; Mysorekar, Indira U.; Mytych, Jennifer; Nabavi, Seyed Mohammad; Nabissi, Massimo; Nagy, Péter; Nah, Jihoon; Nahimana, Aimable; Nakagawa, Ichiro; Nakamura, Ken; Nakatogawa, Hitoshi; Nandi, Shyam S.; Nanjundan, Meera; Nanni, Monica; Napolitano, Gennaro; Nardacci, Roberta; Narita, Masashi; Nassif, Melissa; Nathan, Ilana; Natsumeda, Manabu; Naude, Ryno J.; Naumann, Christin; Naveiras, Olaia; Navid, Fatemeh; Nawrocki, Steffan T.; Nazarko, Taras Y.; Nazio, Francesca; Negoita, Florentina; Neill, Thomas; Neisch, Amanda L.; Neri, Luca M.; Netea, Mihai G.; Neubert, Patrick; Neufeld, Thomas P.; Neumann, Dietbert; Neutzner, Albert; Newton, Phillip T.; Ney, Paul A.; Nezis, Ioannis P.; Ng, Charlene C.w.; Ng, Tzi Bun; Nguyen, Hang T. T.; Nguyen, Long T.; Ni, Hong-min; Ní Cheallaigh, Clíona; Ni, Zhenhong; Nicolao, M. Celeste; Nicoli, Francesco; Nieto-diaz, Manuel; Nilsson, Per; Ning, Shunbin; Niranjan, Rituraj; Nishimune, Hiroshi; Niso-santano, Mireia; Nixon, Ralph A.; Nobili, Annalisa; Nobrega, Clevio; Noda, Takeshi; Nogueira-recalde, Uxía; Nolan, Trevor M.; Nombela, Ivan; Novak, Ivana; Novoa, Beatriz; Nozawa, Takashi; Nukina, Nobuyuki; Nussbaum-krammer, Carmen; Nylandsted, Jesper; O’donovan, Tracey R.; O’leary, Seónadh M.; O’rourke, Eyleen J.; O’sullivan, Mary P.; O’sullivan, Timothy E.; Oddo, Salvatore; Oehme, Ina; Ogawa, Michinaga; Ogier-denis, Eric; Ogmundsdottir, Margret H.; Ogretmen, Besim; Oh, Goo Taeg; Oh, Seon-hee; Oh, Young J.; Ohama, Takashi; Ohashi, Yohei; Ohmuraya, Masaki; Oikonomou, Vasileios; Ojha, Rani; Okamoto, Koji; Okazawa, Hitoshi; Oku, Masahide; Oliván, Sara; Oliveira, Jorge M. A.; Ollmann, Michael; Olzmann, James A.; Omari, Shakib; Omary, M. Bishr; Önal, Gizem; Ondrej, Martin; Ong, Sang-bing; Ong, Sang-ging; Onnis, Anna; Orellana, Juan A.; Orellana-muñoz, Sara; Ortega-villaizan, Maria Del Mar; Ortiz-gonzalez, Xilma R.; Ortona, Elena; Osiewacz, Heinz D.; Osman, Abdel-hamid K.; Osta, Rosario; Otegui, Marisa S.; Otsu, Kinya; Ott, Christiane; Ottobrini, Luisa; Ou, Jing-hsiung James; Outeiro, Tiago F.; Oynebraten, Inger; Ozturk, Melek; Pagès, Gilles; Pahari, Susanta; Pajares, Marta; Pajvani, Utpal B.; Pal, Rituraj; Paladino, Simona; Pallet, Nicolas; Palmieri, Michela; Palmisano, Giuseppe; Palumbo, Camilla; Pampaloni, Francesco; Pan, Lifeng; Pan, Qingjun; Pan, Wenliang; Pan, Xin; Panasyuk, Ganna; Pandey, Rahul; Pandey, Udai B.; Pandya, Vrajesh; Paneni, Francesco; Pang, Shirley Y.; Panzarini, Elisa; Papademetrio, Daniela L.; Papaleo, Elena; Papinski, Daniel; Papp, Diana; Park, Eun Chan; Park, Hwan Tae; Park, Ji-man; Park, Jong-in; Park, Joon Tae; Park, Junsoo; Park, Sang Chul; Park, Sang-youel; Parola, Abraham H.; Parys, Jan B.; Pasquier, Adrien; Pasquier, Benoit; Passos, João F.; Pastore, Nunzia; Patel, Hemal H.; Patschan, Daniel; Pattingre, Sophie; Pedraza-alva, Gustavo; Pedraza-chaverri, Jose; Pedrozo, Zully; Pei, Gang; Pei, Jianming; Peled-zehavi, Hadas; Pellegrini, Joaquín M.; Pelletier, Joffrey; Peñalva, Miguel A.; Peng, Di; Peng, Ying; Penna, Fabio; Pennuto, Maria; Pentimalli, Francesca; Pereira, Cláudia Mf; Pereira, Gustavo J.s.; Pereira, Lilian C.; Pereira De Almeida, Luis; Perera, Nirma D.; Pérez-lara, Ángel; Perez-oliva, Ana B.; Pérez-pérez, María Esther; Periyasamy, Palsamy; Perl, Andras; Perrotta, Cristiana; Perrotta, Ida; Pestell, Richard G.; Petersen, Morten; Petrache, Irina; Petrovski, Goran; Pfirrmann, Thorsten; Pfister, Astrid S.; Philips, Jennifer A.; Pi, Huifeng; Picca, Anna; Pickrell, Alicia M.; Picot, Sandy; Pierantoni, Giovanna M.; Pierdominici, Marina; Pierre, Philippe; Pierrefite-carle, Valérie; Pierzynowska, Karolina; Pietrocola, Federico; Pietruczuk, Miroslawa; Pignata, Claudio; Pimentel-muiños, Felipe X.; Pinar, Mario; Pinheiro, Roberta O.; Pinkas-kramarski, Ronit; Pinton, Paolo; Pircs, Karolina; Piya, Sujan; Pizzo, Paola; Plantinga, Theo S.; Platta, Harald W.; Plaza-zabala, Ainhoa; Plomann, Markus; Plotnikov, Egor Y.; Plun-favreau, Helene; Pluta, Ryszard; Pocock, Roger; Pöggeler, Stefanie; Pohl, Christian; Poirot, Marc; Poletti, Angelo; Ponpuak, Marisa; Popelka, Hana; Popova, Blagovesta; Porta, Helena; Porte Alcon, Soledad; Portilla-fernandez, Eliana; Post, Martin; Potts, Malia B.; Poulton, Joanna; Powers, Ted; Prahlad, Veena; Prajsnar, Tomasz K.; Praticò, Domenico; Prencipe, Rosaria; Priault, Muriel; Proikas-cezanne, Tassula; Promponas, Vasilis J.; Proud, Christopher G.; Puertollano, Rosa; Puglielli, Luigi; Pulinilkunnil, Thomas; Puri, Deepika; Puri, Rajat; Puyal, Julien; Qi, Xiaopeng; Qi, Yongmei; Qian, Wenbin; Qiang, Lei; Qiu, Yu; Quadrilatero, Joe; Quarleri, Jorge; Raben, Nina; Rabinowich, Hannah; Ragona, Debora; Ragusa, Michael J.; Rahimi, Nader; Rahmati, Marveh; Raia, Valeria; Raimundo, Nuno; Rajasekaran, Namakkal-soorappan; Ramachandra Rao, Sriganesh; Rami, Abdelhaq; Ramírez-pardo, Ignacio; Ramsden, David B.; Randow, Felix; Rangarajan, Pundi N.; Ranieri, Danilo; Rao, Hai; Rao, Lang; Rao, Rekha; Rathore, Sumit; Ratnayaka, J. Arjuna; Ratovitski, Edward A.; Ravanan, Palaniyandi; Ravegnini, Gloria; Ray, Swapan K.; Razani, Babak; Rebecca, Vito; Reggiori, Fulvio; Régnier-vigouroux, Anne; Reichert, Andreas S.; Reigada, David; Reiling, Jan H.; Rein, Theo; Reipert, Siegfried; Rekha, Rokeya Sultana; Ren, Hongmei; Ren, Jun; Ren, Weichao; Renault, Tristan; Renga, Giorgia; Reue, Karen; Rewitz, Kim; Ribeiro De Andrade Ramos, Bruna; Riazuddin, S. Amer; Ribeiro-rodrigues, Teresa M.; Ricci, Jean-ehrland; Ricci, Romeo; Riccio, Victoria; Richardson, Des R.; Rikihisa, Yasuko; Risbud, Makarand V.; Risueño, Ruth M.; Ritis, Konstantinos; Rizza, Salvatore; Rizzuto, Rosario; Roberts, Helen C.; Roberts, Luke D.; Robinson, Katherine J.; Roccheri, Maria Carmela; Rocchi, Stephane; Rodney, George G.; Rodrigues, Tiago; Rodrigues Silva, Vagner Ramon; Rodriguez, Amaia; Rodriguez-barrueco, Ruth; Rodriguez-henche, Nieves; Rodriguez-rocha, Humberto; Roelofs, Jeroen; Rogers, Robert S.; Rogov, Vladimir V.; Rojo, Ana I.; Rolka, Krzysztof; Romanello, Vanina; Romani, Luigina; Romano, Alessandra; Romano, Patricia S.; Romeo-guitart, David; Romero, Luis C.; Romero, Montserrat; Roney, Joseph C.; Rongo, Christopher; Roperto, Sante; Rosenfeldt, Mathias T.; Rosenstiel, Philip; Rosenwald, Anne G.; Roth, Kevin A.; Roth, Lynn; Roth, Steven; Rouschop, Kasper M.a.; Roussel, Benoit D.; Roux, Sophie; Rovere-querini, Patrizia; Roy, Ajit; Rozieres, Aurore; Ruano, Diego; Rubinsztein, David C.; Rubtsova, Maria P.; Ruckdeschel, Klaus; Ruckenstuhl, Christoph; Rudolf, Emil; Rudolf, Rüdiger; Ruggieri, Alessandra; Ruparelia, Avnika Ashok; Rusmini, Paola; Russell, Ryan R.; Russo, Gian Luigi; Russo, Maria; Russo, Rossella; Ryabaya, Oxana O.; Ryan, Kevin M.; Ryu, Kwon-yul; Sabater-arcis, Maria; Sachdev, Ulka; Sacher, Michael; Sachse, Carsten; Sadhu, Abhishek; Sadoshima, Junichi; Safren, Nathaniel; Saftig, Paul; Sagona, Antonia P.; Sahay, Gaurav; Sahebkar, Amirhossein; Sahin, Mustafa; Sahin, Ozgur; Sahni, Sumit; Saito, Nayuta; Saito, Shigeru; Saito, Tsunenori; Sakai, Ryohei; Sakai, Yasuyoshi; Sakamaki, Jun-ichi; Saksela, Kalle; Salazar, Gloria; Salazar-degracia, Anna; Salekdeh, Ghasem H.; Saluja, Ashok K.; Sampaio-marques, Belém; Sanchez, Maria Cecilia; Sanchez-alcazar, Jose A.; Sanchez-vera, Victoria; Sancho-shimizu, Vanessa; Sanderson, J. Thomas; Sandri, Marco; Santaguida, Stefano; Santambrogio, Laura; Santana, Magda M.; Santoni, Giorgio; Sanz, Alberto; Sanz, Pascual; Saran, Shweta; Sardiello, Marco; Sargeant, Timothy J.; Sarin, Apurva; Sarkar, Chinmoy; Sarkar, Sovan; Sarrias, Maria-rosa; Sarkar, Surajit; Sarmah, Dipanka Tanu; Sarparanta, Jaakko; Sathyanarayan, Aishwarya; Sathyanarayanan, Ranganayaki; Scaglione, K. Matthew; Scatozza, Francesca; Schaefer, Liliana; Schafer, Zachary T.; Schaible, Ulrich E.; Schapira, Anthony H.v.; Scharl, Michael; Schatzl, Hermann M.; Schein, Catherine H.; Scheper, Wiep; Scheuring, David; Schiaffino, Maria Vittoria; Schiappacassi, Monica; Schindl, Rainer; Schlattner, Uwe; Schmidt, Oliver; Schmitt, Roland; Schmidt, Stephen D.; Schmitz, Ingo; Schmukler, Eran; Schneider, Anja; Schneider, Bianca E.; Schober, Romana; Schoijet, Alejandra C.; Schott, Micah B.; Schramm, Michael; Schröder, Bernd; Schuh, Kai; Schüller, Christoph; Schulze, Ryan J.; Schürmanns, Lea; Schwamborn, Jens C.; Schwarten, Melanie; Scialo, Filippo; Sciarretta, Sebastiano; Scott, Melanie J.; Scotto, Kathleen W.; Scovassi, A. Ivana; Scrima, Andrea; Scrivo, Aurora; Sebastian, David; Sebti, Salwa; Sedej, Simon; Segatori, Laura; Segev, Nava; Seglen, Per O.; Seiliez, Iban; Seki, Ekihiro; Selleck, Scott B.; Sellke, Frank W.; Selsby, Joshua T.; Sendtner, Michael; Senturk, Serif; Seranova, Elena; Sergi, Consolato; Serra-moreno, Ruth; Sesaki, Hiromi; Settembre, Carmine; Setty, Subba Rao Gangi; Sgarbi, Gianluca; Sha, Ou; Shacka, John J.; Shah, Javeed A.; Shang, Dantong; Shao, Changshun; Shao, Feng; Sharbati, Soroush; Sharkey, Lisa M.; Sharma, Dipali; Sharma, Gaurav; Sharma, Kulbhushan; Sharma, Pawan; Sharma, Surendra; Shen, Han-ming; Shen, Hongtao; Shen, Jiangang; Shen, Ming; Shen, Weili; Shen, Zheni; Sheng, Rui; Sheng, Zhi; Sheng, Zu-hang; Shi, Jianjian; Shi, Xiaobing; Shi, Ying-hong; Shiba-fukushima, Kahori; Shieh, Jeng-jer; Shimada, Yohta; Shimizu, Shigeomi; Shimozawa, Makoto; Shintani, Takahiro; Shoemaker, Christopher J.; Shojaei, Shahla; Shoji, Ikuo; Shravage, Bhupendra V.; Shridhar, Viji; Shu, Chih-wen; Shu, Hong-bing; Shui, Ke; Shukla, Arvind K.; Shutt, Timothy E.; Sica, Valentina; Siddiqui, Aleem; Sierra, Amanda; Sierra-torre, Virginia; Signorelli, Santiago; Sil, Payel; Silva, Bruno J. De Andrade; Silva, Johnatas D.; Silva-pavez, Eduardo; Silvente-poirot, Sandrine; Simmonds, Rachel E.; Simon, Anna Katharina; Simon, Hans-uwe; Simons, Matias; Singh, Anurag; Singh, Lalit P.; Singh, Rajat; Singh, Shivendra V.; Singh, Shrawan K.; Singh, Sudha B.; Singh, Sunaina; Singh, Surinder Pal; Sinha, Debasish; Sinha, Rohit Anthony; Sinha, Sangita; Sirko, Agnieszka; Sirohi, Kapil; Sivridis, Efthimios L.; Skendros, Panagiotis; Skirycz, Aleksandra; Slaninová, Iva; Smaili, Soraya S.; Smertenko, Andrei; Smith, Matthew D.; Soenen, Stefaan J.; Sohn, Eun Jung; Sok, Sophia P. M.; Solaini, Giancarlo; Soldati, Thierry; Soleimanpour, Scott A.; Soler, Rosa M.; Solovchenko, Alexei; Somarelli, Jason A.; Sonawane, Avinash; Song, Fuyong; Song, Hyun Kyu; Song, Ju-xian; Song, Kunhua; Song, Zhiyin; Soria, Leandro R.; Sorice, Maurizio; Soukas, Alexander A.; Soukup, Sandra-fausia; Sousa, Diana; Sousa, Nadia; Spagnuolo, Paul A.; Spector, Stephen A.; Srinivas Bharath, M. M.; St. Clair, Daret; Stagni, Venturina; Staiano, Leopoldo; Stalnecker, Clint A.; Stankov, Metodi V.; Stathopulos, Peter B.; Stefan, Katja; Stefan, Sven Marcel; Stefanis, Leonidas; Steffan, Joan S.; Steinkasserer, Alexander; Stenmark, Harald; Sterneckert, Jared; Stevens, Craig; Stoka, Veronika; Storch, Stephan; Stork, Björn; Strappazzon, Flavie; Strohecker, Anne Marie; Stupack, Dwayne G.; Su, Huanxing; Su, Ling-yan; Su, Longxiang; Suarez-fontes, Ana M.; Subauste, Carlos S.; Subbian, Selvakumar; Subirada, Paula V.; Sudhandiran, Ganapasam; Sue, Carolyn M.; Sui, Xinbing; Summers, Corey; Sun, Guangchao; Sun, Jun; Sun, Kang; Sun, Meng-xiang; Sun, Qiming; Sun, Yi; Sun, Zhongjie; Sunahara, Karen K.s.; Sundberg, Eva; Susztak, Katalin; Sutovsky, Peter; Suzuki, Hidekazu; Sweeney, Gary; Symons, J. David; Sze, Stephen Cho Wing; Szewczyk, Nathaniel J.; Tabęcka-łonczynska, Anna; Tabolacci, Claudio; Tacke, Frank; Taegtmeyer, Heinrich; Tafani, Marco; Tagaya, Mitsuo; Tai, Haoran; Tait, Stephen W. G.; Takahashi, Yoshinori; Takats, Szabolcs; Talwar, Priti; Tam, Chit; Tam, Shing Yau; Tampellini, Davide; Tamura, Atsushi; Tan, Chong Teik; Tan, Eng-king; Tan, Ya-qin; Tanaka, Masaki; Tanaka, Motomasa; Tang, Daolin; Tang, Jingfeng; Tang, Tie-shan; Tanida, Isei; Tao, Zhipeng; Taouis, Mohammed; Tatenhorst, Lars; Tavernarakis, Nektarios; Taylor, Allen; Taylor, Gregory A.; Taylor, Joan M.; Tchetina, Elena; Tee, Andrew R.; Tegeder, Irmgard; Teis, David; Teixeira, Natercia; Teixeira-clerc, Fatima; Tekirdag, Kumsal A.; Tencomnao, Tewin; Tenreiro, Sandra; Tepikin, Alexei V.; Testillano, Pilar S.; Tettamanti, Gianluca; Tharaux, Pierre-louis; Thedieck, Kathrin; Thekkinghat, Arvind A.; Thellung, Stefano; Thinwa, Josephine W.; Thirumalaikumar, V.p.; Thomas, Sufi Mary; Thomes, Paul G.; Thorburn, Andrew; Thukral, Lipi; Thum, Thomas; Thumm, Michael; Tian, Ling; Tichy, Ales; Till, Andreas; Timmerman, Vincent; Titorenko, Vladimir I.; Todi, Sokol V.; Todorova, Krassimira; Toivonen, Janne M.; Tomaipitinca, Luana; Tomar, Dhanendra; Tomas-zapico, Cristina; Tomić, Sergej; Tong, Benjamin Chun-kit; Tong, Chao; Tong, Xin; Tooze, Sharon A.; Torgersen, Maria L.; Torii, Satoru; Torres-lópez, Liliana; Torriglia, Alicia; Towers, Christina G.; Towns, Roberto; Toyokuni, Shinya; Trajkovic, Vladimir; Tramontano, Donatella; Tran, Quynh-giao; Travassos, Leonardo H.; Trelford, Charles B.; Tremel, Shirley; Trougakos, Ioannis P.; Tsao, Betty P.; Tschan, Mario P.; Tse, Hung-fat; Tse, Tak Fu; Tsugawa, Hitoshi; Tsvetkov, Andrey S.; Tumbarello, David A.; Tumtas, Yasin; Tuñón, María J.; Turcotte, Sandra; Turk, Boris; Turk, Vito; Turner, Bradley J.; Tuxworth, Richard I.; Tyler, Jessica K.; Tyutereva, Elena V.; Uchiyama, Yasuo; Ugun-klusek, Aslihan; Uhlig, Holm H.; Ułamek-kozioł, Marzena; Ulasov, Ilya V.; Umekawa, Midori; Ungermann, Christian; Unno, Rei; Urbe, Sylvie; Uribe-carretero, Elisabet; Üstün, Suayib; Uversky, Vladimir N; Vaccari, Thomas; Vaccaro, Maria I.; Vahsen, Björn F.; Vakifahmetoglu-norberg, Helin; Valdor, Rut; Valente, Maria J.; Valko, Ayelén; Vallee, Richard B.; Valverde, Angela M.; Van Den Berghe, Greet; Van Der Veen, Stijn; Van Kaer, Luc; Van Loosdregt, Jorg; Van Wijk, Sjoerd J.l.; Vandenberghe, Wim; Vanhorebeek, Ilse; Vannier-santos, Marcos A.; Vannini, Nicola; Vanrell, M. Cristina; Vantaggiato, Chiara; Varano, Gabriele; Varela-nieto, Isabel; Varga, Máté; Vasconcelos, M. Helena; Vats, Somya; Vavvas, Demetrios G.; Vega-naredo, Ignacio; Vega-rubin-de-celis, Silvia; Velasco, Guillermo; Velázquez, Ariadna P.; Vellai, Tibor; Vellenga, Edo; Velotti, Francesca; Verdier, Mireille; Verginis, Panayotis; Vergne, Isabelle; Verkade, Paul; Verma, Manish; Verstreken, Patrik; Vervliet, Tim; Vervoorts, Jörg; Vessoni, Alexandre T.; Victor, Victor M.; Vidal, Michel; Vidoni, Chiara; Vieira, Otilia V.; Vierstra, Richard D.; Viganó, Sonia; Vihinen, Helena; Vijayan, Vinoy; Vila, Miquel; Vilar, Marçal; Villalba, José M.; Villalobo, Antonio; Villarejo-zori, Beatriz; Villarroya, Francesc; Villarroya, Joan; Vincent, Olivier; Vindis, Cecile; Viret, Christophe; Viscomi, Maria Teresa; Visnjic, Dora; Vitale, Ilio; Vocadlo, David J.; Voitsekhovskaja, Olga V.; Volonté, Cinzia; Volta, Mattia; Vomero, Marta; Von Haefen, Clarissa; Vooijs, Marc A.; Voos, Wolfgang; Vucicevic, Ljubica; Wade-martins, Richard; Waguri, Satoshi; Waite, Kenrick A.; Wakatsuki, Shuji; Walker, David W.; Walker, Mark J.; Walker, Simon A.; Walter, Jochen; Wandosell, Francisco G.; Wang, Bo; Wang, Chao-yung; Wang, Chen; Wang, Chenran; Wang, Chenwei; Wang, Cun-yu; Wang, Dong; Wang, Fangyang; Wang, Feng; Wang, Fengming; Wang, Guansong; Wang, Han; Wang, Hao; Wang, Hexiang; Wang, Hong-gang; Wang, Jianrong; Wang, Jigang; Wang, Jiou; Wang, Jundong; Wang, Kui; Wang, Lianrong; Wang, Liming; Wang, Maggie Haitian; Wang, Meiqing; Wang, Nanbu; Wang, Pengwei; Wang, Peipei; Wang, Ping; Wang, Ping; Wang, Qing Jun; Wang, Qing; Wang, Qing Kenneth; Wang, Qiong A.; Wang, Wen-tao; Wang, Wuyang; Wang, Xinnan; Wang, Xuejun; Wang, Yan; Wang, Yanchang; Wang, Yanzhuang; Wang, Yen-yun; Wang, Yihua; Wang, Yipeng; Wang, Yu; Wang, Yuqi; Wang, Zhe; Wang, Zhenyu; Wang, Zhouguang; Warnes, Gary; Warnsmann, Verena; Watada, Hirotaka; Watanabe, Eizo; Watchon, Maxinne; Wawrzyńska, Anna; Weaver, Timothy E.; Wegrzyn, Grzegorz; Wehman, Ann M.; Wei, Huafeng; Wei, Lei; Wei, Taotao; Wei, Yongjie; Weiergräber, Oliver H.; Weihl, Conrad C.; Weindl, Günther; Weiskirchen, Ralf; Wells, Alan; Wen, Runxia H.; Wen, Xin; Werner, Antonia; Weykopf, Beatrice; Wheatley, Sally P.; Whitton, J. Lindsay; Whitworth, Alexander J.; Wiktorska, Katarzyna; Wildenberg, Manon E.; Wileman, Tom; Wilkinson, Simon; Willbold, Dieter; Williams, Brett; Williams, Robin S.b.; Williams, Roger L.; Williamson, Peter R.; Wilson, Richard A.; Winner, Beate; Winsor, Nathaniel J.; Witkin, Steven S.; Wodrich, Harald; Woehlbier, Ute; Wollert, Thomas; Wong, Esther; Wong, Jack Ho; Wong, Richard W.; Wong, Vincent Kam Wai; Wong, W. Wei-lynn; Wu, An-guo; Wu, Chengbiao; Wu, Jian; Wu, Junfang; Wu, Kenneth K.; Wu, Min; Wu, Shan-ying; Wu, Shengzhou; Wu, Shu-yan; Wu, Shufang; Wu, William K.k.; Wu, Xiaohong; Wu, Xiaoqing; Wu, Yao-wen; Wu, Yihua; Xavier, Ramnik J.; Xia, Hongguang; Xia, Lixin; Xia, Zhengyuan; Xiang, Ge; Xiang, Jin; Xiang, Mingliang; Xiang, Wei; Xiao, Bin; Xiao, Guozhi; Xiao, Hengyi; Xiao, Hong-tao; Xiao, Jian; Xiao, Lan; Xiao, Shi; Xiao, Yin; Xie, Baoming; Xie, Chuan-ming; Xie, Min; Xie, Yuxiang; Xie, Zhiping; Xie, Zhonglin; Xilouri, Maria; Xu, Congfeng; Xu, En; Xu, Haoxing; Xu, Jing; Xu, Jinrong; Xu, Liang; Xu, Wen Wen; Xu, Xiulong; Xue, Yu; Yakhine-diop, Sokhna M.s.; Yamaguchi, Masamitsu; Yamaguchi, Osamu; Yamamoto, Ai; Yamashina, Shunhei; Yan, Shengmin; Yan, Shian-jang; Yan, Zhen; Yanagi, Yasuo; Yang, Chuanbin; Yang, Dun-sheng; Yang, Huan; Yang, Huang-tian; Yang, Hui; Yang, Jin-ming; Yang, Jing; Yang, Jingyu; Yang, Ling; Yang, Liu; Yang, Ming; Yang, Pei-ming; Yang, Qian; Yang, Seungwon; Yang, Shu; Yang, Shun-fa; Yang, Wannian; Yang, Wei Yuan; Yang, Xiaoyong; Yang, Xuesong; Yang, Yi; Yang, Ying; Yao, Honghong; Yao, Shenggen; Yao, Xiaoqiang; Yao, Yong-gang; Yao, Yong-ming; Yasui, Takahiro; Yazdankhah, Meysam; Yen, Paul M.; Yi, Cong; Yin, Xiao-ming; Yin, Yanhai; Yin, Zhangyuan; Yin, Ziyi; Ying, Meidan; Ying, Zheng; Yip, Calvin K.; Yiu, Stephanie Pei Tung; Yoo, Young H.; Yoshida, Kiyotsugu; Yoshii, Saori R.; Yoshimori, Tamotsu; Yousefi, Bahman; Yu, Boxuan; Yu, Haiyang; Yu, Jun; Yu, Jun; Yu, Li; Yu, Ming-lung; Yu, Seong-woon; Yu, Victor C.; Yu, W. Haung; Yu, Zhengping; Yu, Zhou; Yuan, Junying; Yuan, Ling-qing; Yuan, Shilin; Yuan, Shyng-shiou F.; Yuan, Yanggang; Yuan, Zengqiang; Yue, Jianbo; Yue, Zhenyu; Yun, Jeanho; Yung, Raymond L.; Zacks, David N.; Zaffagnini, Gabriele; Zambelli, Vanessa O.; Zanella, Isabella; Zang, Qun S.; Zanivan, Sara; Zappavigna, Silvia; Zaragoza, Pilar; Zarbalis, Konstantinos S.; Zarebkohan, Amir; Zarrouk, Amira; Zeitlin, Scott O.; Zeng, Jialiu; Zeng, Ju-deng; Žerovnik, Eva; Zhan, Lixuan; Zhang, Bin; Zhang, Donna D.; Zhang, Hanlin; Zhang, Hong; Zhang, Hong; Zhang, Honghe; Zhang, Huafeng; Zhang, Huaye; Zhang, Hui; Zhang, Hui-ling; Zhang, Jianbin; Zhang, Jianhua; Zhang, Jing-pu; Zhang, Kalin Y.b.; Zhang, Leshuai W.; Zhang, Lin; Zhang, Lisheng; Zhang, Lu; Zhang, Luoying; Zhang, Menghuan; Zhang, Peng; Zhang, Sheng; Zhang, Wei; Zhang, Xiangnan; Zhang, Xiao-wei; Zhang, Xiaolei; Zhang, Xiaoyan; Zhang, Xin; Zhang, Xinxin; Zhang, Xu Dong; Zhang, Yang; Zhang, Yanjin; Zhang, Yi; Zhang, Ying-dong; Zhang, Yingmei; Zhang, Yuan-yuan; Zhang, Yuchen; Zhang, Zhe; Zhang, Zhengguang; Zhang, Zhibing; Zhang, Zhihai; Zhang, Zhiyong; Zhang, Zili; Zhao, Haobin; Zhao, Lei; Zhao, Shuang; Zhao, Tongbiao; Zhao, Xiao-fan; Zhao, Ying; Zhao, Yongchao; Zhao, Yongliang; Zhao, Yuting; Zheng, Guoping; Zheng, Kai; Zheng, Ling; Zheng, Shizhong; Zheng, Xi-long; Zheng, Yi; Zheng, Zu-guo; Zhivotovsky, Boris; Zhong, Qing; Zhou, Ao; Zhou, Ben; Zhou, Cefan; Zhou, Gang; Zhou, Hao; Zhou, Hong; Zhou, Hongbo; Zhou, Jie; Zhou, Jing; Zhou, Jing; Zhou, Jiyong; Zhou, Kailiang; Zhou, Rongjia; Zhou, Xu-jie; Zhou, Yanshuang; Zhou, Yinghong; Zhou, Yubin; Zhou, Zheng-yu; Zhou, Zhou; Zhu, Binglin; Zhu, Changlian; Zhu, Guo-qing; Zhu, Haining; Zhu, Hongxin; Zhu, Hua; Zhu, Wei-guo; Zhu, Yanping; Zhu, Yushan; Zhuang, Haixia; Zhuang, Xiaohong; Zientara-rytter, Katarzyna; Zimmermann, Christine M.; Ziviani, Elena; Zoladek, Teresa; Zong, Wei-xing; Zorov, Dmitry B.; Zorzano, Antonio; Zou, Weiping; Zou, Zhen; Zou, Zhengzhi; Zuryn, Steven; Zwerschke, Werner; Brand-saberi, Beate; Dong, X. Charlie; Kenchappa, Chandra Shekar; Li, Zuguo; Lin, Yong; Oshima, Shigeru; Rong, Yueguang; Sluimer, Judith C.; Stallings, Christina L.; Tong, Chun-kit.
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on...
Tipo: Text
Palavras-chave: Autophagosome; Cance; Rflux; LC3; Lysosome; Macroautophagy; Neurodegeneration; Phagophore; Stress; Vacuole.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00688/79984/83003.pdf

Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples
Provedor de dados: 5
Autores: Schikorski, David; Faury, Nicole; Pepin, Jean-francois; Saulnier, Denis; Tourbiez, Delphine; Renault, Tristan.
Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas.  In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR.
Tipo: Text
Palavras-chave: Ostreid herpesvirus 1; Cupped Pacific oyster; Crassostrea gigas; Experimental infection; Kinetic viral DNA quantification.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00025/13607/10688.pdf

Haemocytes collected from experimentally infected Pacific oysters, Crassostrea gigas: Detection of ostreid herpesvirus 1 DNA, RNA, and proteins in relation with inhibition of apoptosis
Provedor de dados: 5
Autores: Martenot, Claire; Gervais, Ophelie; Chollet, Bruno; Houssin, Maryline; Renault, Tristan.
Recent transcriptomic approaches focused on anti-viral immunity in molluscs lead to the assumption that the innate immune system, such as apoptosis, plays a crucial role against ostreid herpesvirus type 1 (OsHV-1), infecting Pacific cupped oyster, Crassostrea gigas. Apoptosis constitutes a major mechanism of anti-viral response by limiting viral spread and eliminating infected cells. In this way, an OsHV-1 challenge was performed and oysters were monitored at three times post injection to investigate viral infection and host response: 2h (early after viral injection in the adductor muscle), 24h (intermediate time), and 48h (just before first oyster mortality record). Virus infection, associated with high cumulative mortality rates (74% and 100%), was...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00386/49715/50244.pdf

The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA
Provedor de dados: 5
Autores: Chen, Meilin; Kuo, S. T.; Renault, Tristan; Chang, P. H.
A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10...
Tipo: Text
Palavras-chave: Herpesvirus; Abalone; Haliotis diversicolor supertexta; Polymerase chain reaction; Loop-mediated isothermal amplification; SYBR green PCR.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00166/27690/25882.pdf

Toward reference intervals for shellfish: An illustrative case of feeding and respiratory activities in the Pacific cupped oyster,   Crassostrea gigas
Provedor de dados: 5
Autores: François, Cyrille; Haure, Joel; Billy, Jean-christophe; Renault, Tristan.
Background The Quality Assurance and Laboratory Standards Committee of the American Society for Veterinary Clinical Pathology and the guidelines of the Clinical and Laboratory Standards Institute provide a framework for establishing reference intervals of physiological parameters in reputedly healthy individuals, humans, and terrestrial animals, respectively. This framework was applied for the first time to the Pacific cupped oyster, Crassostrea gigas. Reference intervals (RIs) would, first, be of interest for research purposes, including pathophysiology studies. RI determination is the first step before considering the use of RIs for field applications by farmers and marine shellfish health services. Objectives The purpose of this study was to propose...
Tipo: Text
Palavras-chave: Bivalvia; Clearance rate; Health; Ostreidae; Oxygen consumption rate; Reference values.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00627/73913/73286.pdf

New Insight for the Genetic Evaluation of Resistance to Ostreid Herpesvirus Infection, a Worldwide Disease, in Crassostrea gigas
Provedor de dados: 5
Autores: Degremont, Lionel; Lamy, Jean-baptiste; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
The Pacific oyster, Crassostrea gigas, is the most important commercial oyster species cultivated in the world. Meanwhile, the ostreid herpesvirus 1 (OsHV-1) is one of the major pathogens affecting the Pacific oyster, and numerous mortality outbreaks related to this pathogen are now reported worldwide. To assess the genetic basis of resistance to OsHV-1 infection in spat C. gigas and to facilitate breeding programs for such a trait, if any exist, we compared the mortality of half- and full-sib families using three field methods and a controlled challenge by OsHV-1 in the laboratory. In the field, three methods were tested: (A) one family per bag; (B) one family per small soft mesh bag and all families inside one bag; (C) same as the previous methods but...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/38066/36178.pdf

Concomitant herpes-like virus infections in hatchery-reared larvae and nursery-cultured spat Crassostrea gigas and Ostrea edulis
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Concomitant sporadic high mortalities were reported in France in May 1994 among batches of hatchery-reared larval Pacific oysters Crassostrea gigas and European flat oysters Ostrea edulis in 2 hatcheries, and in June and July 1994 among batches of cultured spat of both species in a shellfish nursery. Histological observation showed the presence of cellular abnormalities in moribund animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in infected larvae and spat of both oyster species. This is the first description of a herpes-like virus infection in larval O.. edulis. Viruses observed in diseased larvae and spat of both species are similar with respect to ultrastructure and morphogenesis, They were detected...
Tipo: Text
Palavras-chave: Apoptosis; Virus replication; Concomitant infection; Oyster mortality; Crassostrea gigas; Pacific oyster; Ostrea edulis; European flat oyster; Herpes like virus.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-820.pdf

Bonamia-like parasite found in the Suminoe oyster Crassostea rivularis reared in France
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea. Thus,...
Tipo: Text
Palavras-chave: Bonamia like parasite; Bonamiosis; Simunoe oyster; Crassostrea rivularis.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-2904.pdf

New Trends in Important Diseases Affecting the Culture of Fish and Molluscs in the ICES Area 2002– 2015
Provedor de dados: 5
Autores: Alfjorden, A; Areskog, M; Bruno, D; Carnegie, R; Cheslett, D; Feist, S; Ford, S; Jones, S; Lillehaug, Atle; Madsen, L; Renault, Tristan; Ruane, N; Vennerstrom, P.
The ICES Working Group on Pathology and Diseases of Marine Organisms (WGPDMO) provides annual reviews of national reports on the disease status of wild and farmed fish and molluscs in the ICES area. In 2004, the group published a first report collating this information from 1998-2002. This second report aims to provide an update on the status of the major diseases described in the original report and also to provide an overview of new diseases which have emerged since the previous report was published.
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00585/69731/67620.pdf

Recherches d'ADN d'hepès virus infectant les mollusques dans les populations d'huîtres en milieu naturel et dans leur environnement
Provedor de dados: 5
Autores: Pepin, Jean-francois; Solliec, Gaelle; Vigneron, Vassilia; Thebault, Anne; Lamothe, Julien; Renault, Tristan.
The presence of a herpes-type virus has been demonstrated in various species of marine shellfish (bivalves and gastropoda) throughout different regions of the world (Europe, North and South America, Asia, Australia), and is frequently associated with high mortality levels. Nevertheless, apart from the sanitary monitoring in mollusc farming, very little data is available about the detection of this type of virus in the natural populations of bivalves and about the persistence of such viruses in sea water. Various diagnostic tools have been developed in order to detect the oyster herpes virus (Ostreid herpesvirus, OsHV). The presence of this virus has been looked for by PCR in marine water samples coming from fattening ponds, in order to precise the natural...
Tipo: Text
Palavras-chave: Levures; Eau de mer; Diagnostic; Huîtres; Mollusques; OsHV; Herpèsvirus.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3716.pdf

Study of atrazine effects on Pacific oyster, Crassostrea gigas, haemocytes
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Bouilly, Karine; Lapegue, Sylvie; Thomas-guyon, H.
Shellfish farming is an important economic activity around the world. This activity often takes place in areas subjected to various recurring pollutions. The recrudescent use of herbicides in agriculture including atrazine implies pollutant transfer towards aquatic environment in estuarine areas. Harmful effects of such substances on animals in marine environment, particularly on cultured bivalves, are poorly documented. Bivalve molluscs such as mussels and oysters have been postulated as ideal indicator organisms because of their way of life. They filter large volumes of seawater and may therefore accumulate and concentrate eontaJ.11inants within their tissues. Moreover, development of techniques allowing effect analysis of such compounds on bivalve...
Tipo: Text
Palavras-chave: Crassostrea gigas; Haemocytes; Flow cytometry; Atrazine; Cellular activities; Toxicity.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2827.pdf

Infection with the protozoan parasite Bonamia ostreae modifies in vitro haemocyte activities of flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan.
Bonamia ostreae is an intracellular protozoan parasite, infecting haemocytes of the European flat oyster Ostrea edulis. Oyster defence mechanisms mainly rely on haemocytes. In the present study in vitro interactions between parasites and flat oyster haemocytes were investigated using flow cytometry and light microscopy. Haemocyte parameters including: non specific esterase activity, reactive oxygen species (ROS) production and phagocytosis were monitored using flow cytometry after 2 hour cell incubation with live and dead B. ostreae. Two ratios of parasites per haemocyte were tested (5:1 and 10:1), haemocytes alone were used as controls and the experiment was carried out three times. Flow cytometry revealed a decrease of non specific esterase activities...
Tipo: Text
Palavras-chave: Reactive oxygen species (ROS); Esterase; Flow cytometry; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6271.pdf

Experimental infection of Pacific oyster Crassostrea gigas spat by ostreid herpesvirus 1: demonstration of oyster spat susceptibility
Provedor de dados: 5
Autores: Schikorski, David; Renault, Tristan; Saulnier, Denis; Faury, Nicole; Moreau, Pierrick; Pepin, Jean-francois.
In 2008 and 2009, acute mortalities occurred in France among Pacific cupped oyster, Crassostrea gigas, spat. Different hypothesis including the implication of environmental factors, toxic algae and/or pathogens have been explored. Diagnostic tests indicated that OsHV-1 including a particular genotype, termed OsHV-1 mu Var, was detected in most of samples and especially in moribund oysters with the highlighting of virus particles looking like herpes viruses by TEM examination. In this study, an experimental protocol to reproduce OsHV-1 infection in laboratory conditions was developed. This protocol was based on the intramuscular injection of filtered (0.22 mu m) tissue homogenates prepared from naturally OsHV-1 infected spat collected on French coasts...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00037/14795/12104.pdf

Contribution à l'étude de mollusques marins : Iridovirus-like et Herpèsvirus-like. Description, caractérisation biochimique, cycle de multiplication viral, diagnostic et étude épidémiologique
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses...
Tipo: Text
Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf

Effects of temperature and salinity on the survival of Bonamia ostreae parasite infecting falt oyster Ostrea edulis in sea water
Provedor de dados: 5
Autores: Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could be demonstrated between the development of the disease and environmental parameters such as temperature or salinity. The parasite life cycle, including its survival outside the host is not completely known. Nevertheless, the infection can be directly transmitted by cohabitation between infected and non infected oysters suggesting that the parasite does not need intermediate host to complete its cycle. In the present study, the impact of temperature and salinity on the survival of purified parasites...
Tipo: Text
Palavras-chave: Effects; Temperarature; Salinity; Bonamia ostreae; Parasite; Pathology; Flat oyster; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3375.pdf

Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters
Provedor de dados: 5
Autores: Diaz, Seila; Renault, Tristan; Villalba, Antonio; Carballal, María Jesús.
Disseminated neoplasia (DN) has been detected in cockles from various beds in Galicia (NW Spain). A study was performed to characterise cockle neoplastic cell ultrastructure and to evaluate the effect of this disease at different severity stages on various haemolymph cell parameters. Examination of cockle neoplastic cells with transmission electron microscopy (TEM) showed round shapes and a lack of pseudopods, a high nucleus:cytoplasm diameter ratio, Golgi complexes, abundant mitochondria, ribosomes, and numerous endoplasmic reticulum tubes and electron-lucent vesicles. Various haemolymph cell parameters (cell mortality, non-specific esterase and lysosome biovolume, reactive oxygen intermediates [ROI] production, phagocytosis ability, intracellular Ca2+...
Tipo: Text
Ano: 2011
URL: https://archimer.ifremer.fr/doc/00043/15468/12846.pdf

Facteurs de risque d'apparition et d'émergence des maladies infectieuses en aquaculture
Provedor de dados: 5
Autores: Renault, Tristan; Guichard, B.
In the last few years, the global increase of aquatic animal production and farmed species has been associated with an increase in the number and spread of infectious diseases. In 2002-2003, 62 cases of emerging diseases were reported in aquatic animals, two thirds in aquacultured species, mainly in Europe and Northern America. A disease is emerging when its incidence significantly increases in a given time, place and population. To emerge, a disease has to be introduced in a free   zone or a free population, and animals must be exposed to favouring transmission factors. The risks of introducing new diseases are mainly associated to movements of infected animals (farmed or feral, dead or alive), contaminated farming gear or transport media,  evolution of...
Tipo: Text
Palavras-chave: Facteurs d'exposition; Animaux aquatiques; Maladies émergentes.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2977.pdf

Rapport d'activité 1994 du Laboratoire Génétique, Aquaculture et Pathologie de La Tremblade - Bouin
Provedor de dados: 5
Autores: Gerard, Andre; Baud, Jean-pierre; Renault, Tristan; Goulletquer, Philippe.
Rapport d'activité 1994 du Laboratoire Génétique, Aquaculture et Pathologie de La Tremblade et Bouin.
Tipo: Text
Palavras-chave: Génétique; Pathologie; Invertébrés marins; Rapport Activité.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00071/18252/15815.pdf

Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection
Provedor de dados: 5
Autores: Leprêtre, Maxime; Faury, Nicole; Segarra, Amelie; Claverol, Stéphane; Degremont, Lionel; Palos-ladeiro, Mélissa; Armengaud, Jean; Renault, Tristan; Morga, Benjamin.
Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and...
Tipo: Text
Palavras-chave: Proteomics; Interactions; Antiviral response; OsHV-1; Pacific oysters.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00669/78075/80307.pdf

Juvenile oyster disease (JOD) in American oysters, Crassostrea virginica, reared in France. Detection of abnormal calcium deposits in gill and mantle tissues
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared during 1992 and 1993, to test their growth performances. These assays were performed to investigate the possible replacement of Crassostrea gigas with a non indigenous species, Crassostrea virginica. During the summer of 1993, sporadic mass mortalities (80-90%) occurred among young, Crassastrea virginica, oysters reared in the IFREMER nursery in La Tremblade (Charente Maritime).
Tipo: Text
Palavras-chave: Histology; Crassostrea virginica; Mortality; Oysters.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2975.pdf

Episodes de mortalité massive de moules bleues observés en 2014 dans les Pertuis charentais
Provedor de dados: 5
Autores: Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan.
Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen
Tipo: Text
Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf

Rapport d'activités 2014 de l'Unité SG2M. Santé, Génétique et Microbiologie des mollusques
Provedor de dados: 5
Autores: Renault, Tristan; Lapegue, Sylvie; Le Guyader, Soizick; Hatt, Philippe-jacques; Rivet, Florence.
L’environnement marin est un système dynamique soumis à une forte variabilité d’origines naturelle et anthropique. Les pressions d’origine anthropique sont diverses (surexploitations des ressources vivantes, rejets de contaminants, aménagements, changements globaux) et ont fortement augmenté aux cours des dernières décennies, générant des évolutions importantes dans les écosystèmes marins et dans les usages qui en dépendent, en particulier en zone côtière. L’amélioration du bon état écologique et des services rendus par les écosystèmes marins pose des questions complexes en termes de fonctionnalité et de biodiversité, de durabilité de l’exploitation des ressources vivantes, des politiques publiques et des interactions entre usages. Ces questions entrent...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00283/39455/37921.pdf

Two Vibrio splendidus related strains collaborate to kill Crassostrea gigas: taxonomy and host alterations
Provedor de dados: 5
Autores: Gay, Melanie; Renault, Tristan; Pons, Anne-marie; Le Roux, Frédérique.
For several years, strains phenotypically related to Vibrio splendidus have been associated with mortality outbreaks of molluscs. A former study on Crassostrea gigas demonstrated the genetic diversity of V splendidus strains associated with diseased animals. Another study suggested that different strains may act in an additive/synergistic way leading to higher C. gigas mortality rates. Here, a strain pair (31+32) was characterised at taxonomic and virulence levels. Using a polyphasic approach, these strains were confirmed to be V splendidus-related, without a clear discrimination between V. kanaloae and V. pomeroyi since hybridisation rates with both these strains were above 70%. Following experimental infection of C. gigas by injection in the adductor...
Tipo: Text
Palavras-chave: Histopathology; Molecular taxonomy; Agonism; Vibriosis.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2912.pdf

Effects of temperature on herpes-like virus detection among hatchery-reared larval Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Le Deuff, Rose-marie; Renault, Tristan; Gerard, Andre.
This paper describes effects of temperature on herpes-like virus detection in Pacific oyster Crassostrea gigas larvae held at different temperatures. Intranuclear, intracytoplasmic and extracellular viral particles were observed in velum and mantle connective tissues of oyster larvae reared at 25-26°C. In larvae held at 22-23OC, although nuclear lesions were observed, the presence of viral particles was not detected. Results were obtained for oyster larvae with 4 different parental origins. Herpesvirus infection was found in 3 of the 4 groups of oyster larvae held In the same conditions at the higher temperature.
Tipo: Text
Palavras-chave: Temperature; Mortality; Larval culture; Crassotrea gigas; Pacific oyster; Herpesvirus.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/1996/publication-2900.pdf

Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin.
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members...
Tipo: Text
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf

Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Duchemin, M; Auffret, Mathieu; Thomas-guyon, Helene; Renault, Tristan.
Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Phagocytosis; Hemocytes; Flow cytometry.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3898.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 95 RPC R 62 - Programme Pathologie - Rapport Scientifique année 1995
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Thuillier, Benoit.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00043/15453/12828.pdf

Evidence for interspecies transmission of oyster herpesvirus in marine bivalves
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Lipart, Cécile; Davison, Andrew.
Since 1991, numerous herpesvirus infections associated with high mortality have been reported around the world in various marine bivalve species. In order to determine whether these infections are due to ostreid herpesvirus-l (OsHV1), a previously characterized pathogen of the Japanese oyster (Crassostrea gigas), PCR analysis was carried out on 30 samples of larvae collected from four bivalve species (C. gigas, Ostrea edulis, Ruditapes decussatus and Ruditapes philippinarum), most exhibiting mortality prior to collection. All samples were shown to be infected by OsHV1. Viral genomes in three samples of C. gigas and three of R. philippinarum that originated from the same hatchery were unusual in bearing a deletion of at least 2.8 kbp in an inverted repeat...
Tipo: Text
Palavras-chave: Bivalve; Crassostrea gigas; Herpesvirus; Japanese oyster; Transmission.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-705.pdf

Surmortalités de la moule bleue Mytilus edulis dans les Pertuis Charentais (mars 2014)
Provedor de dados: 5
Autores: Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre.
Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf

Molecular and cellular characterization of apoptosis in flat oyster a key mechanisms at the heart of host-parasite interactions
Provedor de dados: 5
Autores: Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle.
Bonamia ostreae has been associated with the decline of flat oyster Ostrea edulis populations in some European countries. This obligatory intracellular parasite persists and multiplies into hemocytes. Previous in vitro experiments showed that apoptosis is activated in hemocytes between 1 h and 4 h of contact with the parasite. The flat oyster uses the apoptosis pathway to defend against B. ostreae. However, the parasite might be also able to modulate this response in order to survive in its host. In order to investigate this hypothesis the apoptotic response of the host was evaluated using flow cytometry, transmission electron microscopy and by measuring the response of genes involved in the apoptotic pathway after 4 h. In parallel, the parasite response...
Tipo: Text
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00454/56540/58246.pdf

Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Gagnaire, Beatrice; Bonnard, Marc; Thomas-guyon, Helene; Renault, Tristan; Miramand, Pierre; Lapegue, Sylvie.
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oleron bay (Charente-Maritime, France; 50 ng L-1) and the second was 10 times higher (500 ng L-1). Exposure to 50 ng L-1 cadmium caused a significant...
Tipo: Text
Palavras-chave: Phenoloxidase; Flow cytometry; Hemocytes; Aneuploidy; Crassostrea gigas; Cadmium.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1687.pdf

Vibriosis in aquaculture. 16th EAFP Conference, Tampere, Finland, 4th September 2013
Provedor de dados: 5
Autores: Haenen, O. L. M.; Fouz, B.; Amaro, C.; Isern, M. M.; Mikkelsen, H.; Zrncic, S.; Travers, Marie-agnes; Renault, Tristan; Wardle, R.; Hellstrom, A.; Dalsgaard, I.
Aquaculture in brackish and marine water is growing worldwide (FAO, 2014), including new cultured species. Various Vibrio infections occur, and cause significant problems, in fish, crustacean and shellfish (Austin &amp; Austin, 2007. Vibrio anguillarum, V. salmonicida, V. ordalii and V. vulnificus are among the pathogens that lead to the biggest losses in aquaculture all over the world (Toranzo et al. 2005, Sandlund et al., 2010; Sitjà-Bobadilla et al. 2014).
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00211/32186/30615.pdf

Sensitivity of mortality reporting by the French oyster farmers
Provedor de dados: 5
Autores: Lupo, Coralie; Osta Amigo, Axel; Mandard, Y. V.; Peroz, C.; Arzul, Isabelle; Francois, Cyrille; Garcia, Celine; Renault, Tristan.
As diseased shellfish show only very seldom symptoms, any mortality event can be a disease suspicion. In theory, monitoring of mortality events in shellfish can thus provide a tool for early detection of infectious diseases in order to implement effective control measures. This consists in passive mortality event reporting by shellfish farmers to the local competent authority. Sensitivity of the detection of mortality events is a key quality indicator for this type of surveillance. Since 2008, increased mortality outbreaks occur in Pacific oysters Crassostrea gigas in France, leading to economic losses. It is important to evaluate how much the outbreaks have altered the quality indicators of the reporting system and if this modification is long-standing,...
Tipo: Text
Palavras-chave: Early detection; Passive surveillance; Aquaculture; Capture-recapture methods.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00102/21343/18966.pdf

Chlamydia-like organisms in ctenidia and mantle cells of the Japanese oyster Crassostrea ggias from the French Atlantic coast
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie.
Grossly visible lesions of the ctenidia and occasional mortalities in Japanese oyster Crassostrea gigas from the Atlantic French coast were reported in 1992-1993. From August 1992 to March 1993, 235 living oysters were collected to determine the cause of tissue lesions and mortalities. Microscopical examination of fixed, stained sections revealed that 53 2% of these oysters had ctenidia and mantle lesions associated with irregular basophllic and Feulgen positive cytoplasmic inclusion bodies. Ultrastructurally, the infectious agent was a chlamydia-like organism. Indirect fluorescent and peroxidase conjugated antibody tests suggested that this agent might share common antigens with the prokaryotic agent Chlamydia psittaci, strain ovis
Tipo: Text
Palavras-chave: Mantle; Ctenidia; Chlamydia like organisms; Pacific oyster; Crassostrea gigas.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/publication-2896.pdf

Characterization of Vibrio isolated from Crassostrea gigas spat suffering summer mortality outbreaks
Provedor de dados: 5
Autores: Gay, Melanie; Renault, Tristan; Le Roux, Fredérique.
The French oyster production is mainly based on the pacific oyster, Crassos/rea gigas. However, for several years, C. gigas spat has suffered severe mortality outbreaks. A multifactorial etiology implicating environment, oyster physiology, genetics and pathogens is heavily suspected. A herpesvims and bacteria belonging to the Vihrio s\p/endidus polyphyletic group have been isolated From summer mortality outbreaks. Moreover, several strains belonging to the genus Vibrio have been identified as pathogen for different mollusk species. However, few tools are available for the identification of these Vibrios and for the characterization of the virulence of the pathogenic strains.
Tipo: Text
Palavras-chave: Mortality; Vibrio splendidus; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2854.pdf

In vitro research of anti-HSV-1 activity in different extracts from Pacific oysters Crassostrea gigas
Provedor de dados: 5
Autores: Olicard, Cecile; Didier, Yohann; Marty, Christel; Bourgougnon, Nathalie; Renault, Tristan.
Mortalities related to the detection of Ostreid Herpesvirus 1 (OsHV-1) have been previously reported in France among larvae and spat of the Pacific oyster Crassostrea gigas. Adult oysters appear less sensitive to herpesvirus infections, although OsHV-1 has been detected in adults without signs of disease or mortality. This suggests that the virus is able to persist in its host and that adult oysters may be able to control OsHV-1 infection. Little is known about antiviral substances in invertebrates. The present work concerns the research of antiviral substances in adult oyster C. gigas, where putative antiviral activities were monitored using 3 strategies: (1) in metabolites with variable polarity, (2) in peptidic extracts and (3) in crude haemolymph. In...
Tipo: Text
Palavras-chave: Haemolymph; Antiviral peptides; HSV 1; OsHV 1; Antiviral defence; Crassostrea gigas; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2922.pdf

Essai d'acclimatation de Crassostrea virginica et performances biologiques comparées avec Crassostrea gigas (2ème partie)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Heurtebise, Serge; Nourry, Max; Renault, Tristan; Cochennec, Nathalie; Zanette, Yvan; Blachier, Philippe.
Deuxième volet du programme d'essai d'acclimatation de l'huître américaine Crassostrea virginica, ce rapport présente les actions et les résultats de l'année 1993 qui a été consacrée au suivi des performances comparées de croissance des populations de C. gigas et C. virginica produites en 1992, ceci en trois sites différents: • dans le bassin de Marennes-Oléron en claire et sur estran, à la station IFREMER de Bouin en élevage intensif, au CREAA sur l'Ile d'Oléron en marais extensif. Au terme de cette seconde année d'étude, il est d'ores et déjà possible d'affirmer que que la souche anglaise de C. virginica étudiée ne présente pas de caractéristiques de croissance susceptibles de la rendre compétitive par rapport au témoin Crassostrea gigas et cela d'autant...
Tipo: Text
Palavras-chave: Mollusques; Acclimatation; Crassostrea virginica; Crassostrea gigas; Génétique; Pathologie.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00077/18846/16419.pdf

Etude pluridiciplinaire de la DYNAmique des MOrtalités estivales dans le bassin de Marennes- Oléron : Dynamique des mortalités en relation avec la reproduction in situ et la température - Suivi de paramètres hémocytaires - Synergie écologie côtière et activité hémocytaire
Provedor de dados: 5
Autores: Renault, Tristan; Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Guilpain, Patrice; Geairon, Philippe; Razet, Daniel; Seugnet, Jean-luc; Gagnaire, Beatrice; Kerdudou, Nolwenn.
Massive abnormal mortalities of flat oysters Crassostrea during the summer period are nothing new, and such episodes have been described in scientific literature for about forty years. Numerous studies demonstrate that there is not one single identified cause to this phenomenon and that it is, on the contrary, a combination of different parameters. This program proposes a multidisciplinary approach to the problem of summer mortality of C. gigas in which the working hypothesis is that these mortalities result from the interaction of three parameters: environment, host and pathogens. As a consequence, this program gathers all the necessary competences for the study of these interactions: competences in genetics, physiology, immunology, ecotoxicology,...
Tipo: Text
Palavras-chave: Marennes Oléron; Activité hémoctyaire; Synergie écologie côtière; Pathologie; Paramètres hémocytaires; Physiologie; Paramètres; Reproduction; Mortalités; Crassostrea gigas; Huîtres.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-3404.pdf

Exploring First Interactions Between Ostreid Herpesvirus 1 (OsHV-1) and Its Host, Crassostrea gigas: Effects of Specific Antiviral Antibodies and Dextran Sulfate
Provedor de dados: 5
Autores: Martenot, Claire; Faury, Nicole; Morga, Benjamin; Degremont, Lionel; Lamy, Jean-baptiste; Houssin, Maryline; Renault, Tristan.
Viral entry mechanisms of herpesviruses constitute a highly complex process which implicates several viral glycoproteins and different receptors on the host cell surfaces. This initial infection stage was currently undescribed for Ostreid herpes virus 1 (OsHV-1), a herpesvirus infecting bivalves including the Pacific oyster, Crassostrea gigas. To identify OsHV-1 glyproteins implicated in the attachment of the virus to oyster cells, three viral putative membrane proteins, encoded by ORF 25, 41, and 72, were selected and polyclonal antibodies against these targets were used to explore first interactions between the virus and host cells. In addition, effects of dextran sulfate, a negative charged sulfated polysaccharide, were investigated on OsHV-1 infection....
Tipo: Text
Palavras-chave: OsHV-1; Antiviral antibodies; Dextran sulfate; Interaction; Crassostrea gigas; Infection.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00501/61273/64847.pdf

Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf

Bilan 2009 du réseau REPAMO - Réseau national de surveillace de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Cobret, Laetitia; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Breerette, Stephane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Banamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00086/19711/17352.pdf

Rapid and sensitive detection of ostreid herpesvirus 1 in oyster samples by real-time PCR
Provedor de dados: 5
Autores: Pepin, Jean-francois; Riou, Antoine; Renault, Tristan.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region. The melt curve analysis of OsHV-1 DNA or DNA extracted from infected material showed only one melting temperature peak (75.75 ± 0.1 °C). The assay had a detection...
Tipo: Text
Palavras-chave: SYBR® Green; Real time PCR; Crassostrea gigas; Ostreid herpesvirus 1; Herpesvirus.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3951.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular immunological and cellular tools "VINO". Second periodic progress report: 4th January 2000 at 3rd January 2001
Provedor de dados: 5
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using teclmiques developed by the patiners to characterise viruses by studying their genome and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpesvirus (OsHV-1) DNA sequence with determination of the genome structure. 2 - Comparing OsHV -1 with viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure. 3 -...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00048/15947/13377.pdf

Etude de l'herpès virus de l'huître - Fonds d'Aide à la Recherche et à l'Innovation - Décision d'Aide à la Recherche n° 95-07-01 - Rapport Final Août 1998
Provedor de dados: 5
Autores: Renault, Tristan.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en œuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En effet,...
Tipo: Text
Palavras-chave: Virus; Pathologie; Herpès virus; Crassostrea gigas; Pathogène; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15451/12826.pdf

Isolation and primary culture of gill and digestive gland cells from the common mussel Mytilus edulis
Provedor de dados: 5
Autores: Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry.
As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater...
Tipo: Text
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf

Bonamia ostreae, parasite of the European flat oyster, Ostrea edulis, does not experimentally infect the Japanese oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Grizel, Henri.
The protozoan Bonamia ostreae (Ascetospora) is the etiological agent of bonamiosis, or hemocyte disease of the European fiat oyster, Ostrea edulis (Pichot et al., 1980). Since this infection was first reported from the coast of Brittany (Comps et al., 1980), the parasite has spread rapidly along the French Atlantic coasts (Grizel &amp; Tigé, 1982), and in the European countries including the UK, Holland, lreland and Spain (Bannister &amp; Key, 1982; Van Banning, 1982; Polanco et al., 1984). Under the EEC Council Directive 91/67, this parasitic protozoan is included in list II of Annex A. Serious pathogen agents for molluscs that should be obligatorily declared are placed in this list
Tipo: Text
Palavras-chave: Infection; Protozoan; Oyster; Ostrea edulis; Bonamia ostreae.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/publication-2892.pdf

Putative antiviral activity in hemolymph from adult Pacific oysters, Crassostrea gigas
Provedor de dados: 5
Autores: Olicard, Cecile; Renault, Tristan; Torhy, Corinne; Benmansour, Abdenour; Bourgougnon, Nathalie.
Innate, non-specific resistance mechanisms are important to pathogens, particularly for delaying virus replication at the onset of infection, Innate immunity constitutes the first line of defense in vertebrates and is the only one in invertebrates. Little is known about possible antiviral substances in invertebrates. The present work concerns a Study of antiviral substances in hemolymph from adult Crassostrea gigas oysters. Despite the detection of cytotoxicity in fresh filtered hemolymph for both mammalian (CC50: 750 mu g/ml) and fish cells (CC50: &gt; 2000 mu g/ml for EPC cells and 345 mu g/ml for RTG-2 cells), an antiviral substance was detected. Fresh filtered hemolymph was capable of inhibiting the replication of herpes simplex virus type I in vitro...
Tipo: Text
Palavras-chave: Hemolymph; VHSV; IPNV; HSV; Antiviral defense; Crassosirea gigas; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-774.pdf

Etude du virus de type herpes observé chez les huîtres
Provedor de dados: 5
Autores: Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Delsert, Claude; Cochennec, Nathalie; Renault, Tristan.
Since 1991, sporadic, but high mortality in larvae of the Pacific oyster (Crassostrea gigas) has been observed during the summer in various French hatcheries. This mortality was concomitant with the detection of a virus by transmission electron microscopy. This virus, by virtue of its morphological characteristics and developmental cycle, seems to belong to the Herpesviridae family. The pathogenicity of this viral agent has been demonstrated in the laboratory by experimentally induced mortality on healthy larvae. Moreover, concomitant mortality has been reported in larvae of Crassostrea gigas and Ostrea edulis reared in the same tanks, with detection of a herpes-type virus.
Tipo: Text
Palavras-chave: Virus; Herpeviridae; Pathologie; Crassostrea gigas.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3248.pdf

Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review
Provedor de dados: 5
Autores: Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf

Etude des mouvements calciques dans les hémocytes de l'huître creuse Crassostrea gigas lors de leur immunostimulation : 1ères approches expérimentales
Provedor de dados: 5
Autores: Aton, Emilie; Cognard, C.; Renault, Tristan; Thomas-guyon, Hélène; Imbert, N.
Bivalve mollusc farms are continuously exposed to variations of factors either natural (variation of salinity and temperature) or from man (heavy metals, HAP, phytosanitary) likely to alter the defence systems of bivalve molluscs. These alterations would explain, in certain cases, their sensitivity vis-à-vis certain marine pathogens and could in part be at the source of the episodes of massive mortalities seen periodically in oyster and mussel larval populations. That is why many studies concerning the contaminants' immunotoxicity are currently in progress.
Tipo: Text
Palavras-chave: Polluant; Calicum; Hemocytes; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2855.pdf

Note de synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Avril-Mai 1996
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile; Le Deuff, Rose-marie; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade dans le cadre de l'étude du virus de type herpès et de la mise au point de méthodes de diagnostic fait suite à la précédente note du 16 avril 1996. Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont donnés et commentés en sept points. 1- Analyses Pays de Loire 2 - Bilan des analyses réalisées dans le cadre du protocole IFREMER BOUIN/IFREMER ARGENTON/SODABO 3 - Bilan global des analyses Pays de Loire et Bouin-Argenton-SODABO 4 - Analyses effectuées en PCR pour la recherche de virus de type herpès sur des lots prélevés en 1996, dans le...
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15499/12867.pdf

Bonamia-like parasite found in the Suminoe oyster Crassostrea rivularis reared in France
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production. Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-Like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea....
Tipo: Text
Palavras-chave: Crassostrea rivularis; Suminoe oyster; Bonamiosis; Bonamia-like parasite.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00446/55795/62652.pdf

Bilan 2013 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Tourbiez, Delphine; Chollet, Bruno; Faury, Nicole; Haffner, Philippe; Dubreuil, Christine; Serpin, Delphine; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Cheve, Julien; Penot, Julia; Le Gal, Dominique; Lebrun, Luc; Le Gac-abernot, Chantal; Langlade, Aime; Bedier, Edouard; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrick; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Repamo a pour objectif de détecter précocement les infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage.  L’étude des hausses de mortalités a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la collecte d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités d’huîtres creuses (Crassostrea gigas) (44 interventions) ont été recensées dans la majorité des bassins de production de mai à...
Tipo: Text
Palavras-chave: Réseau Surveillance; Pathologie; Mollusques; Coquillages; Santé.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00197/30798/29167.pdf

Survey for neoplasia in Macoma balthica from the Gulf of Gdansk by flow cytometry
Provedor de dados: 5
Autores: Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek.
Using flow cytometry, 234 Macoma balthica were examined during a survey to determine frequency of neoplasia in the Gulf of Gdansk (Poland). Clams were collected in 4 locations and DNA content in gill tissue cells was determined by flow cytometry using propidium iodide staining. Cell permeabilization was induced by osmotic shock. Prevalence of neoplasia ranged from 9.6 to 26.7 % depending on location. DNA content in aneuploid cells was higher than in normal dividing cells. The fluorescence value for aneuploid cells corresponded to tetraploid/pentaploid cells. Three stages of neoplasia were defined, based on the percentage of aneuploid cells determined by flow cytometry. Histopathological and cytogenetic analyses were also carried out on the same clams for...
Tipo: Text
Palavras-chave: Prevalence; Macoma balthica; Flow cytometry; Gill neoplasia.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2919.pdf

Recherche d'effets biologiques de l'atrazine sur hémocytes d'huître creuse, Crassostrea gigas, in vivo et in vitro
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Thomas-guyon, Hélène; Lapegue, Sylvie; Bouilly, Karine; Renault, Tristan.
In the last decades, shellfish farming and in particular ostreiculture have developed in a significant way in France. Marennes-Oleron (Charente-Maritime) basin is the first French producer of oysters. But this area is also subjected to many recurring pollution, brought mainly by the Charente river.  The recrudescence using of herbicides in agriculture including atrazine implies the transfer towards the aquatic environment of new pollutants in estuarine areas. Moreover industrial rejections bring many heavy metals in these same estuarine areas.  It is appropriate of wondering on harmful effects of all these pollutants on the animals living in these areas, particularly oysters.  Bivalve molluscs have been postulated as ideal indicator organisms because of...
Tipo: Text
Palavras-chave: Atrazine; Flow cytometry; Haemocytes; Crassostrea gigas; Atrazine; Cytométrie de flux; Hémocytes; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-834.pdf

Appearance and spread of diseases among bivalve molluscs in the northern hemisphere in relation to international trade
Provedor de dados: 5
Autores: Renault, Tristan.
Bivalve mollusc culture is a well-developed marine aquaculture activity in many countries around the world, notably in the northern hemisphere. During the development of this activity, numerous countries have been confronted with infectious diseases of varying severity and duration. Research has been conducted to determine the aetiology, epidemiology and control measures for these epizootics. Major epizootics in bivalve molluscs have been caused by viruses, bacteria, fungi and protozoan parasites. Moreover, important commercial relations exist in marine mollusc culture between different geographical areas. This must be taken into account in explaining the appearance and the spread of some infectious diseases in several countries around the world. The...
Tipo: Text
Palavras-chave: Northern hemisphere; International trade; Infectious diseases; Bivalve molluscs; Mollusques bivalves; Maladies infectieuses; Hémisphère Nord; Commerce internatioanal.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/1996/publication-2913.pdf

Detection of a mollicute-like organism in kuruma shrimp, Penaeus japonicus
Provedor de dados: 5
Autores: Choi, Dong-lim; Park, Myoung-ae; Sohn, Sang-gyu; Heo, Moon-soo; Renault, Tristan.
A filamentous form of mollicute-like bacterium was detected during a routine health survey of wild adult kuruma shrimp Penaeus japonicus. The kuruma shrimp were native to Japan and were imported to Korea. The histology showed no pathological changes. Transmission electron microscopy revealed extensive infections of hepatopancreatic epithelial cells by a pleomorphic, filamentous intracellular bacterium. The filamentous bacterium was of about 60 nm in diameter and 300 nm to more than 1µm in length. The morphology of bacteria were filamentous and branched with terminal blebs, or knobs, on the branches. They lacked the cell wall, and were bounded by the plasma membrane. They contained typical prokaryotic ribosomes and fibrillar DNA-like strands. No additional...
Tipo: Text
Palavras-chave: Transmission electron microscopy; Hepatopancreas; Mollicute; Intracellular bacteria; Penaeus japonicus.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/1996/publication-2901.pdf

Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri
Provedor de dados: 5
Autores: Ren, Weicheng; Chen, Haixia; Renault, Tristan; Cai, Yuyong; Bai, Changming; Wang, Chongming; Huang, Jie.
Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb,...
Tipo: Text
Palavras-chave: Acute viral necrosis virus; Herpesvirus; OsHV-1; Genome; Scallop; Chlamys farreri; AVNV.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00135/24620/22650.pdf

Surveillance du milieu marin et protection santiaire des coquillages
Provedor de dados: 5
Autores: Renault, Tristan.
Shellfish farming is a major economic activity in the European Union, France being one of the main producing countries. Regulations were introduced to meet society's current expectations in terms of food safety at the fishing, farming and harvesting stages, as weil as during processing and marketing. Toxic algae, pathogens or pollutants may contaminate shellfish products during production and harvesting, partly because of the particular environ mental conditions in which these animais are grown. Monitoring programmes were thus deve/oped to detect the presence of biological and chemical contaminants in coastal waters and in shellfish, and to monitor the food safety of the latter.
Tipo: Text
Palavras-chave: Pollutants; Microbiological contamination; Toxic algae; Shellfish; Marine environment; Programmees; Monitoring; Polluants; Contamination microbiologique; Algues toxiques; Coquillages; Milieu marin; Réseaux; Surveillance.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6932.pdf

Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes
Provedor de dados: 5
Autores: Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre.
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials...
Tipo: Text
Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf

Viruses infecting bivalve molluscs
Provedor de dados: 5
Autores: Renault, Tristan; Novoa, Beatriz.
Bivalve molluscs are filter feeders and as a consequence they may bioaccumulate in their tissues viruses that infect humans and higher vertebrates. However, there have also been described mortalities of bivalve molluscs associated with viruses belonging to different families. Mass mortalities of adult Portuguese oysters, Crassostrea angulata, among French livestocks (between 1967 and 1973) were associated with irido-like virus infections. Herpesviruses were reported in the eastern oyster, Pacific oyster and European flat oyster and lately in scallops and clams. Disseminated neoplasia, a proliferative cell disorder of the circulatory system of bivalves, although of uncertain aetiology, has been suggested to be caused by retroviral infections. Other viruses...
Tipo: Text
Palavras-chave: Infection; Virus; Molluscs; Bivalves.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-2910.pdf

Combination of a pesticide exposure and a bacterial challenge: In vivo effects on immune response of Pacific oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan.
To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR)....
Tipo: Text
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf

Expression de gènes viraux de l'herpès virus OsHV-1 sous sa forme µVar chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Segarra, Amelie; Faury, Nicole; Haffner, Philippe; Pepin, Jean-francois; Tourbiez, Delphine; Trancart, Suzanne; Travers, Marie-agnes; Renault, Tristan.
Le premier cas d'infection à virus de type herpès a été rapporté en 1972, aux Etats Unis, chez l'huître américaine, Crassostrea virginica (Farley et al, 1972). A partir de 1991, des mortalités massives associées à la détection de virus de type herpes ont été observées chez plusieurs espèces de bivalves marins, à différents stades de développement (essentiellement chez les larves et le naissain) et dans différentes régions du monde. Sur la base d’observation en microscopie électronique à transmission, ces virus présentaient des caractéristiques morphologiques identiques à celles des Herpesvirus (Roizman, 1982).  Les connaissances acquises concernant la taille, la structure et le séquençage du génome d’un virus infectant les larves d’huîtres creuses en...
Tipo: Text
Palavras-chave: Herpès virus OsHV-1; Expression gènes; Huître creuse; Crassostrea gigas.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00195/30627/29076.pdf

Bilan 1999 du Réseau REPAMO - Réseau National de Surveillance zoosanitaire des Mollusques marins
Provedor de dados: 5
Autores: Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Grizel, Henri; Berthe, Franck; Cochennec, Nathalie; Renault, Tristan; Le Roux, Frederique.
Le réseau REPAMO (Réseau de Pathologie des Mollusques) est un réseau de santé animale, qui s'occupe des coquillages marins du littoral français métropolitain. Les mollusques marins des côtes françaises représentent une ressource par la pêche, mais sont surtout une part importante de l'aquaculture. Les pertes liées aux maladies dans ce domaine d'activité sont aujourd'hui considérées comme l'un des principaux facteurs limitants de son développement au niveau mondial. Deux exemples récents suffisent à illustrer ce danger pour l'ostréiculture française : • La culture de l''huître plate Ostrea edulis, déjà atteinte par un parasite, Marteilia refringens, est passée en quelques années de 20 000 tonnes à 1800 tonnes, suite à une épizootie. En effet à la fin des...
Tipo: Text
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00098/20903/18522.pdf

Experimental herpes-like viral infections in marine bivalves: demonstration of interspecies transmission
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Lipart, Cecile.
Since 1972, herpes-like virus infections have been reported in several marine bivalve species around the world. Viral detection was often associated with high mortality rates in larvae and spat. To determine whether a single virus is able to infect different bivalve host species, we carried out experimental transmission assays. As a first step, 8 assays were performed to infect axenic Crassostrea gigas larvae with virus from infected C. gigas larvae using a previously described protocol. The protocol appeared reliable and PCR was confirmed as a powerful technique for detecting viral DNA in experimentally infected oysters. The defined protocol was then applied to infect different bivalve species. Interspecies viral transmission was demonstrated under...
Tipo: Text
Palavras-chave: Experimental infection; Host specificty; Interspecies transmission; Marine bivalves; Herpes like virus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-819.pdf

Les virus infectant les mollusques marins : un exemple d'actualité, les herpèsvirus
Provedor de dados: 5
Autores: Renault, Tristan.
Although viruses have been associated with mortality outbreaks in different mollusc species, little information is available on their exact affiliation and taxonomic position. Herpes viruses have attracted particular attention because of their economic and ecological impact on both farmed and wild marine molluscs over the past 20 years. Investigations based on molecular approaches have been carried out on the herpes virus infecting Pacific oysters in France. Molecular and ultra-structural data were used to classify the virus under the name ostreid herpesvirus 1 (OsHV-1), as the first known species in the family Malacoherpesviridae. Since 2008, massive mortality outbreaks were reported in several Crassostrea gigas oyster farms in France, Ireland, and the...
Tipo: Text
Palavras-chave: Molluscs; Pacific cupped oyster; Herpes virus; Ostreid herpesvirus 1; Excess mortality.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00069/18000/15562.pdf

Space-time clustering of mortality notifications in Pacific oysters of Charente sluices, France, 2008-2010
Provedor de dados: 5
Autores: Lupo, Coralie; Mandard, Yann-vari; Arzul, Isabelle; Francois, Cyrille; Garcia, Celine; Renault, Tristan; Bareille, Nathalie.
Current monitoring of exceptional mortality events of oysters in France consists in passive surveillance, leading to delayed investigation of causation by scientists and management by policy makers. Early detection of clusters of mortality events in time and space would increase possibilities for their investigation and management. A space-time cluster analysis of existing mortality notifications in Pacific oysters was conducted in France, Charente sluices, from 2008 to 2010. As an example of potential demographic confounder of clustering, the analyses were adjusted on oyster age. Several clusters were identified in space and time each studied year. Patterns of mortality notifications were consistent with the introduction and spread of a contagious disease...
Tipo: Text
Palavras-chave: Notification; Oyster; Mortality; Cluster analysis; Saptiotemporal.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00058/16915/14393.pdf

Ecophysiological study on the Pacific oyster Crassostrea gigas naturally infected by a Chlamydia-like microorganism : effect of infection level and diet on oyster physiological responses
Provedor de dados: 5
Autores: Soletchnik, Patrick; Goulletquer, Philippe; Cochennec, Nathalie; Renault, Tristan; Geairon, Philippe.
A population of Pacific ovsters, Crassostrea gigas, infected with a Chlamydia-like procaryote was reared to compare infection trends under three different feeding conditions: a starved condition, an  intermediate condition with a diet of Skeletonema costatllm, and a mixed algal diet. Several physiological and pathological criteriJ: presence of gill abnormality; microscopic examination, basophilic intracytoplasmic inclusions, Feulgen reaction; condition index, filtration, food consumptiQn; fecal production and respiration were used to compare the various modalities one month after the initiation of the experiment. The Irodality of starved oysters showed reduce physiological performances in terms of scope for growth and condition index compared to oysters...
Tipo: Text
Palavras-chave: Pathogen; Chlamydia; Procaryote; Crassostrea gigas; Oyster; Ecophysiology; Pathogène; Chlamydia; Procaryote; Crassostrea gigas; Huître; Ecophysiologie.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-2741.pdf

Bilan 2012 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Pepin, Jean-francois; Hatt, Philippe-jacques; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Huchet, Eve; Dubreuil, Christine; Chollet, Bruno; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Annezo, Jean-pierre; Terre-terrillon, Aouregan; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Hittier, Benoist; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à réglementés et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font partie des missions institutionnelles de l’IFREMER.   L’étude des hausses de mortalités (protocole II) a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la réalisation d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités...
Tipo: Text
Palavras-chave: Réseau; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00143/25470/23625.pdf

Rapport sur la situation zoosanitaire du cheptel Crassostrea gigas : cas du bassin de Marennes-Oléron
Provedor de dados: 5
Autores: Grizel, Henri; Renault, Tristan; Cochennec, Nathalie; Tige, Gilbert; Audic, Guylaine; Pichot, Yves; Chollet, Bruno; Goulletquer, Philippe; Soletchnik, Patrick; Geairon, Philippe; Prou, Jean.
Le cheptel d'huîtres creuses, Crassostrea gigas, du Bassin de Marennes-Oléron a subi ces dernières années des morta lités (mai 88, mai 91, été 1992 et hiver 92-93). Les examens histopathologiques pratiqués durant celles-ci n'ont pas permis de déceler d'agents pathogènes. Les causes avaient été attribuées essentiellement, pour les premiers cas, à un manque de nourriture corrélé avec des conditions de sécheresse (donc un manque d'apport d'eau douce pour le bassin) et à des surcharges trop importantes des biomasses de fiItreurs, huîtres y compris. L'explication du dernier cas de mortalité survenue au cours de l'hiver serait d'un autre ordre: l'abaissement brutal des salinités, notamment dans les claires où étaient épurées soit des huîtres natives fragilisées,...
Tipo: Text
Palavras-chave: Etude zoosanitaire; Mortalité; Epidémiologie; Pathologie; Marennes Oléron.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00050/16152/13635.pdf

A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors
Provedor de dados: 5
Autores: Nasfi, H.; Travers, Marie-agnes; De Lorgeril, Julien; Habib, C.; Sannie, T.; Sorieul, L.; Gerard, J.; Avarre, J. C.; Haffner, Philippe; Tourbiez, Delphine; Renault, Tristan; Furones, D.; Roque, A.; Pruzzo, C.; Cheslett, D.; Gdoura, R.; Vallaeys, T.
The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase...
Tipo: Text
Palavras-chave: VNTR; Vibrio splendidus; Epidemiology; Vsm; OmpU; Crassotrea giga; Mortality.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00255/36666/35350.pdf

Identification of genes from flat oyster Ostrea edulis as suitable housekeeping genes for quantitative real time PCR
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan.
Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases.  Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their...
Tipo: Text
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf

Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Bado Nilles, A; Gagnaire, Beatrice; Thomas-guyon, Helene; Le Floch, S; Renault, Tristan.
The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24 h contamination period and 10 of them showed significant modulations of...
Tipo: Text
Palavras-chave: Toxicity; PAHs; Pacific oyster; Spectrophotometry; Flow cytometry; Haemocyte activity.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4696.pdf

Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro
Provedor de dados: 5
Autores: Hellio, Claire; Bado Nilles, Anne; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Helene.
The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), beta-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at...
Tipo: Text
Palavras-chave: Prophenoloxidase; Phenoloxidase; Oyster; Immunity; Defence; Crassostrea gigas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2926.pdf

Herpesvirus observé chez les huîtres - Résultats août 1995/avril 1996
Provedor de dados: 5
Autores: Renault, Tristan.
Les travaux concernant le virus de type herpès, observé chez les huîtres, ont été poursuivis en 1995-1996 et ont ainsi permis d'aboutir à la mise au point d'un protocole de purification du virus, malgré l'ensemble des difficultés rencontrées (impossibilité de reproduire le virus sur lignées cellulaires, dépendance vis à vis des professionnels pour l'approvisionnement en matériel infecté et difficulté de planifier les expériences). De ce fait, le point de blocage que représentait l'obtention de particules virales en nombre suffisant pour le développement d'outils de diagnostic fiables et sensibles a été levé.
Tipo: Text
Palavras-chave: Herpès virus; Pathologie; Virus; Mortalité; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00050/16153/13636.pdf

Programme Pathologie - Rapport scientifique Année 1994 - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 94 RPC R 62
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno; Maffart, Patricia; Haffner, Philippe; Berthe, Franck; Thuillier, Benoit.
Le but de ce programme pathologie est de recherher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassastrea gigas, afin d'identifier d'éventuels agents causant des mortalité, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socioéconomique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la pathologie....
Tipo: Text
Palavras-chave: Pathologie; Agent pathogène; Infection; Virus; Herpèsvirus; Purification; Huître creuse; Crassostrea gigas.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00044/15502/12874.pdf

Ostreid herpesvirus 1 detection and relationship with Crassostrea gigas spat mortality in France between 1998 and 2006
Provedor de dados: 5
Autores: Garcia, Celine; Thebault, Anne; Degremont, Lionel; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno; Francois, Cyrille; Joly, Jean-pierre; Ferrand, Sylvie; Kerdudou, Nolwenn; Renault, Tristan.
Since its molecular characterisation, Ostreid herpesvirus 1 (OsHV-1) has been regularly detected in Crassostrea gigas in France. Although its pathogenicity was demonstrated on larval stages, its involvement during mortality outbreaks at the juvenile stage was highly suspected but not evidenced. To investigate mortality outbreaks, the French National Network for Surveillance and Monitoring of Mollusc Health (REPAMO) carried out two surveys in juvenile C. gigas. The first survey lasted from 1998 to 2006 and was an epidemiological inquiry occurring when oyster farmers reported mortality outbreaks. The second survey, a longitudinal one, was set up in 1998 to complete the network observations on OsHV-1. Data analysis showed a specific pattern of mortality...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15110/12572.pdf

Environmental stress in the pacific oyster Crassostrea gigas during gametogenesis : role of herbicides?
Provedor de dados: 5
Autores: Burgeot, Thierry; Quirion, Sophie; Quiniou, Francoise; Knoery, Joel; Soletchnik, Patrick; Gagnaire, Beatrice; Renault, Tristan; Osada, M.; Geret, F.; Communal, P. Y.; Jadas-hecart, A.; Gagne, F.
In the Atlantic French coast, significant mortalities were observed in oyster communities during gametogenesis. These deaths possibly result from the cumulative effects of various stressors such as seasonal temperature variations, pesticide application and the release of contaminants from the resuspension of sediments. A field survey, based on aquaculture conditions at 15 and 70 cm from the sediment bed with genetically-resistant (R) and -sensitive (S) lines, showed a stress condition. Significant weight loss was observed during May 14th through June 10th for both R and S lines. On one hand, the levels of the egg yolk vitellin in females were decreased at May 14th for both R and S lines.  On the other hand, a significant increase in vitellin production was...
Tipo: Text
Palavras-chave: Mortalities; Herbicides; Sediment; Biomarkers; Gametogenesis; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3384.pdf

In vivo effects of metaldehyde on Pacific oyster, Crassostrea gigas: comparing hemocyte parameters in two oyster families.
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf

Induction of apoptosis by UV in the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle.
Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that...
Tipo: Text
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf

Rapport d'activité 1995 du Laboratoire Génétique, Aquaculture et Pathologie de La Tremblade - Bouin
Provedor de dados: 5
Autores: Gerard, Andre; Goulletquer, Philippe; Baud, Jean-pierre; Renault, Tristan.
Rapport d'activité 1995 du GAP (Laboratoire Génétique, Aquaculture, Pathologie) de La Tremblade.
Tipo: Text
Palavras-chave: Pathologie; Génétique; Aquaculture; Invertebres marins; Physiologie; Rapport activités.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00071/18253/15816.pdf

Environment and immunomodulation : effects of the soluble fraction of Norwegian heavy fuel on the immune system of Sea bass, Dicentrarchus labrax
Provedor de dados: 5
Autores: Bado-nilles, Anne; Quentin, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Auffret, Michel; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood.  The aim of the present work was to detect the impact in vivo of PAHs on the immune...
Tipo: Text
Palavras-chave: PaHs; Fish; Contamination; Dicentrarchus labrax.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3427.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 98 RPC R 7 - Programme Pathologie - Rapport Final année 1998
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf

Mortalités massives de l'huître creuse - Rapport final du programme de recherche sur l'étude de la cinétique de détection d'agents infectieux associés à des épisodes de mortalités de naissains d'huîtres creuses Crassostrea gigas sur un site ostréicole de Marennes-Oléron (CIDAGINF 2009-2010)
Provedor de dados: 5
Autores: Saulnier, Denis; Pepin, Jean-francois; Guesdon, Stephane; Degremont, Lionel; Faury, Nicole; Haffner, Philippe; Renault, Tristan; Travers, Marie-agnes; Tourbiez, Delphine; Geairon, Philippe; Le Moine, Olivier; Seugnet, Jean-luc; Soletchnik, Patrick.
This study aims to improve understanding of causes of mortalities in oyster Crassostrea gigas in pertuis charentais related to environmental parameters and the kinetics of targeted infectious agents, such as bacteria Vibrio splendidus (group ) and V. aestuarianus and Ostreid herpesvirus OsHV-1. Two sampling campaigns were carried out on the site of Agnas in 2009 and 2010, before, during or following mortality events of spat from March to September. The approach is to detect and quantify in short time step infectious agents from tissues of farmed oysters placed on rack and in various samples taken nearby: native oysters and other species (wild mussels and barnacles), seawater and sediment. With physical and chemical automatic probes placed on the site,...
Tipo: Text
Palavras-chave: Huître creuse; Crassostrea gigas; Mortalité; Virus herpès; Vibrions; Environnement; Pertuis Charentais; Pacific oyster; Crassostrea gigas; Mortality; Herpes virus; Vibrios; Environment; Pertuis Charentais.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00058/16920/14400.pdf

Development of TaqMan real-time PCR assays for monitoring Vibrio harveyi infection and a plasmid harbored by virulent strains in European abalone Haliotis tuberculata aquaculture
Provedor de dados: 5
Autores: Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis.
The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial...
Tipo: Text
Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf

Bilan 2008 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Tourbiez, Delphine; Omnes, Emmanuelle; Cobret, Laetitia; Faury, Nicole; Haffner, Philippe; Saulnier, Denis; Pepin, Jean-francois; Renault, Tristan; Antajan, Elvire; Rauflet, Fabienne; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00086/19709/17351.pdf

Fonds d'Aide à la Recherche et à l'Innovation (FARI) - Décision d'Aide à la Recherche n° 95-07-01 - Rapport final
Provedor de dados: 5
Autores: Renault, Tristan.
Depuis 1991 , des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae.  Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre.  Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text
Palavras-chave: Mollusques bivalves; Pathologie; Mortalités; Herpes virus; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00166/27715/25910.pdf

Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids
Provedor de dados: 5
Autores: Batista, Frederico M.; Lopez-sanmartin, Monserrat; Boudry, Pierre; Navas, Jose I.; Ruano, Francisco; Renault, Tristan; Fonseca, Vera G.; Leitao, Alexandra.
Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid...
Tipo: Text
Palavras-chave: Aneuploidy; Ostreid herpesvirus 1; Oyster; Crassostrea.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00215/32581/35759.pdf

A flow cytometric approach to study intracellular-free Ca2+ in Crassostrea gigas haemocytes
Provedor de dados: 5
Autores: Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N.
Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium.   Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were...
Tipo: Text
Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf

Défis de la surveillance des maladies chez les coquillages marins en France
Provedor de dados: 5
Autores: Lupo, Coralie; Francois, Cyrille; Arzul, Isabelle; Garcia, Celine; Joly, Jean-pierre; Renault, Tristan.
The basic concepts and objectives of animal disease surveillance are common to all types of animal production. Yet, the procedures for implementation have been mostly developed for terrestrial animal productions. Disease surveillance in marine shellfish calls for specific measures in view of the peculiarities of marine biology and rearing techniques. The main objective of disease surveillance in marine shellfish is to detect as early as possible exotic and newly emerging diseases. With this objective in mind, a national surveillance network for surveillance of mollusc diseases called Repamo has been developed. It combines passive reporting and risk-based surveillance.
Tipo: Text
Palavras-chave: Surveillance; Coquillage; Milieu marin; Détection précoce; Epidémiologie; Surveillance; Shellfish; Marine environment; Early detection.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00109/22023/19653.pdf

Establishing causal link between an infectious agent and mortalities in marine molluscan aquaculture on the example of Bonamia ostreae and Herpèsvirosis in oysters : proposal of a causal grid analysis
Provedor de dados: 5
Autores: Thebault, Anne; Cochennec, Nathalie; Arzul, Isabelle; Renault, Tristan.
In marine molluscan aqauculture, infectious agents are regularly cited in literature associated with mass mortalities as emergin diseases, but the causal link is often based on very few argumeents. 24 ways to demonstrate causal links from epidemiolgical, ecological and marine pathology literature feasible for marine molluscs are given. Those criteria were applied to the ewample of Bonamia ostreae for Ostrea edulis and Herpesvirus for spats and larvae of Crassostrea gigas. Three principles could be applied in marine field for choosing criteria wich should be taken in priority : the demonstration must be given at three levels of organization, organ individual, population, the demonstration must keeps maximum epidemiological criterias, and then mixes...
Tipo: Text
Palavras-chave: Herpesvirus; Bonamia ostrea; Crassostrea gigas; Ostrea edulis; Marine pathology; Ecology; Epidemiology; Infectious agents; Molluscan aquaculture.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3341.pdf

Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae
Provedor de dados: 5
Autores: Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Tipo: Text
Palavras-chave: DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2916.pdf

Complementary approaches to diagnosing marine diseases: a union of the modern and the classic
Provedor de dados: 5
Autores: Burge, Colleen A.; Friedman, Carolyn S.; Getchell, Rodman; House, Marcia; Lafferty, Kevin D.; Mydlarz, Laura D.; Prager, Katherine C.; Sutherland, Kathryn P.; Renault, Tristan; Kiryu, Ikunari; Vega-thurber, Rebecca.
Linking marine epizootics to a specific etiology is notoriously difficult. Recent diagnostic successes show that marine disease diagnosis requires both modern, cutting-edge technology (e.g. metagenomics, quantitative realtime PCR) and more classic methods (e.g. transect surveys, histopathology and cell culture). Here, we discuss how this combination of traditional and modern approaches is necessary for rapid and accurate identification of marine diseases, and emphasize how sole reliance on any one technology or technique may lead disease investigations astray. We present diagnostic approaches at different scales, from the macro (environment, community, population and organismal scales) to the micro (tissue, organ, cell and genomic scales). We use disease...
Tipo: Text
Palavras-chave: Marine disease; Aetiology; Diagnostics; Marine epizootics.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00314/42536/41911.pdf

New insights in flat oyster Ostrea edulis resistance against the parasite Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality in flat oyster stocks in Europe. Control of the disease currently relies on disease management practices and transfer restriction. Previously, massal selections based on survival to challenge to infection with B. ostreae have been applied to produce flat oyster families with resistant progeny. In an attempt to understand the molecular mechanisms involved in disease resistance, differentially expressed sequence tags between resistant and wild Ostrea edulis haemocytes, both infected with the parasite, were identified using suppression subtractive hybridisation. Expression of seven ESTs has been studied using quantitative reverse-transcriptase PCR. The base-line...
Tipo: Text
Palavras-chave: Protozoan; Ostrea edulis; Flat oyster; Transcriptomic; Gene expression.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00071/18218/15790.pdf

Bilan 2010 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Guichard, Benjamin; Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Brerette, Stéphane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam; Cantin, Christian.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l’IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l’huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00059/17050/14599.pdf

Genomics and mollusc pathogens: trends and perspective
Provedor de dados: 5
Autores: Renault, Tristan.
Global mollusc production is continuously increasing and reached 12.8 million tonnes in 2004 representing 28.2 percent of the total world production valued at US$ 9.4 billions. However, cultivated molluscs may suffer from severe mortality outbreaks. Among the possible causes is the occurrence of infectious diseases due to a variety of pathogens including protozoan parasites, bacteria and viruses. Preventing and controlling diseases has become a priority for aquaculture sustainability. However, molluscs are unique in terms of health management. Molluscs like other invertebrates, lack a true adaptative immune system. Vaccination can not be used to protect them against pathogens. Moreover, molluscs are usually reared in the open sea which strongly limits the...
Tipo: Text
Palavras-chave: Diagnosis; Phylogeny; Genomics; Mollluscs; Pathogens.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4574.pdf

Impact de l'Erika sur la pathologie de bivalves d'intérêt commercial: résultats préliminaires
Provedor de dados: 5
Autores: Thebault, Anne; Robert, Maeva; Renault, Tristan; Dumais, M.; Goulletquer, Philippe.
Après le naufrage de l'Erika, la question s'est rapidement posée de l'impact des hydrocarbures sur la santé des coquillages, car la pollution concernait des zones de production conchylicoles. Le suivi zoosanitaire en place dans ces zones permettait d'avoir des données de référence avant impact. Il a été choisi différents sites exposés de référence, qui ont fait l'objet d'analyse en toxicologie et en pathologie. A la différence du cas de l'Arnoco Cadiz, l'effort n'a pas été mis uniquement sur les huîtres mais aussi sur d'autres espèces de coquillages, comme les moules et des fouisseurs, particulièrement exposés comme les coques et les palourdes. La mesure de l'exposition effectuée par la Direction de l'Environnement Littoral (MONERlKA) montre qu'une...
Tipo: Text
Palavras-chave: Pollution; Pathologie; Hydrocarbures; Coquillages; HAP; Histologie; Finistère; Vendée.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00087/19826/17468.pdf

Improving early detection of exotic or emergent oyster diseases in France: identifying factors associated with shellfish farmer reporting behaviour of oyster mortality
Provedor de dados: 5
Autores: Lupo, Coralie; Osta-amigo, Axel; Mandard, Yann-vari; Peroz, Carole; Renault, Tristan.
Farmers’ vigilance is essential for the detection of epidemics, including potential emerging diseases, in marine shellfish. A field study was conducted to investigate oyster farmers’ reporting practices and behaviour, and to identify factors influencing the reporting process of oyster mortality, with the ultimate aim of improving early detection of unexplained oyster mortality outbreaks.  A retrospective case-control study of oyster farmers from Charente-Maritime (France) was designed, based on interviews with 27 non-reporting and 89 reporting farmers, further split into 40 formerly-reporting and 49 currently-reporting farmers. Information about farmer and farm characteristics, farming practices, farm health history and related financial compensation on...
Tipo: Text
Palavras-chave: Case-control study; Ordinal logistic regression; Surveillance; Notification; Shellfish diseases.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00190/30132/28594.pdf

Disseminated neoplasms in bivalves
Provedor de dados: 5
Autores: Alderman, Dj; Green, M; Balouet, G; Renault, Tristan; Ford, Susan.
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00585/69739/67628.pdf

Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures
Provedor de dados: 5
Autores: Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie.
The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days....
Tipo: Text
Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf

Infection herpétiques chez les invertébrés : détection de virus de type herpès chez les mollusques bivalves marins
Provedor de dados: 5
Autores: Renault, Tristan.
Among the infectious diseases observed in bivalve molluscs, viral infections are often not well known because of a certain inadequacy of the diagnostic techniques usually applied during instances of mortality. In fact, photonic microscopy was still the base method for analysing specimens in several laboratories working on pathologies in molluscs. This technique is insufficient in the event of a pathology of viral etiology if it is not supplemented by other approaches such as the electronic microscope, research of cytopathogenic effects on cell lines or detection of the infectious agent with the help of specific reagents (specific antibodies and nucleic probes). Moreover, since no bivalve mollusc cell line is currently available, in vitro research of...
Tipo: Text
Palavras-chave: Virologie; Mollusques; Détection; Maladie; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-2903.pdf

Infestation of the cupped oysters Crassostrea angulata, C. gigas and their first-generation hybrids by the copepod Myicola ostreae: differences in susceptibility and host response
Provedor de dados: 5
Autores: Batista, F. M.; Boudry, Pierre; Dos Santos, A.; Renault, Tristan; Ruano, F.
We Studied the prevalence and intensity of the parasitic copepod Myicola ostreae in 2 closely related oysters Crassostrea angulata and C. gigas and their F1 hybrids. The effects on host and host reaction were also analysed to better understand host-parasite relationships between copepods and bivalve molluscs. Full reciprocal crosses were carried out between C. angulata and C. gigas and the progenies were reared in the wild in Ria Formosa Lagoon (Portugal), allowing natural infestation by M. ostreae. Prevalence and intensity were significantly higher in C. angulata than in C. gigas. The parasite level of F1 hybrids was similar to C. angulata and significantly higher than in C. gigas. The results of our study support a hypothesis of dominantly inherited...
Tipo: Text
Palavras-chave: Encapsulation; Host reaction; Host parasite relationship; Dominance inherence; Parasitism; Hybridization; Copepods; Oysters.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6442.pdf

Marteiliosis in American oysters Crassostrea virginica reared in France
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno.
Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared at 3 different locations. A survey was taken of these young C, virginica oysters during 1993. Histological and electron microscopical examination revealed a parasite interpreted as a Marteilia-like protozoan in 1 oyster. The structure and the initial stages of the developmental cycle of this parasite are described for the first time in American oysters. However, no mature spores were detected. Thus, exact identification of the protozoan is difficult. This study indicates that American oysters could be infected with Marteilia spp, during cohabitation with Marteilia-infected native bivalves in France.
Tipo: Text
Palavras-chave: American oyster; Crassostrea virginica; Marteiliosis; Marteilia-like parasite.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00258/36926/36177.pdf

Essai d'acclimatation de Crassostrea virginica et performances biologiques comparées avec Crassostrea gigas (1ère partie)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Nourry, Max; Renault, Tristan; Cochennec, Nathalie.
Premier volet du programme d'essai d'acclimatation de l'huître américaine Crassostrea virginica, ce rapport présente les actions et les résultats de l'année 1992 qui a été consacrée à : • l'importation selon les normes du CIEM d'un lot de 50 huîtres en provenance d'Angleterre, • leur élevage en salle de quarantaine et leur conditionnement en vue d'une accélération de la maturation sexuelle, • l'obtention d'une première génération, ainsi que son suivi en élevage larvaire et en micronurserie à l'URGE-La Tremblade, • au contrôle des performances comparées des deux populations de C. virginica et de C. gigas en nurserie de prégrossissement à l'URRA-Bouin pendant toute la période automnale et hivernale. Au terme de cette première année d'étude il apparait que la...
Tipo: Text
Palavras-chave: Mollusques; Acclimatation; Crassostrea virginica; Crassostrea gigas; Génétique; Pathologie.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00077/18843/16417.pdf

Etude des intéractions entre le parasite Bonamia ostreae et son hôte, huître plate Ostrea edulis in vitro (Diaporama)
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan.
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in...
Tipo: Text
Palavras-chave: Intéraction; Hémocytes; Ostrea edulis; Bonamia ostreae; Parasitologie.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3366.pdf

Detection and description of a particular Ostreid herpesvirus 1 genotype associated with massive mortality outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008
Provedor de dados: 5
Autores: Segarra, Amelie; Pepin, Jean-francois; Arzul, Isabelle; Morga, Benjamin; Faury, Nicole; Renault, Tristan.
Ostreid herpesvirus 1 (OsHV-1) infections have been reported around the world and are associated with high mortalities of the Pacific oyster (Crassostrea gigas). In the summer 2008, abnormal mortality rates ranging from 80% to 100% were reported in France and affected only Pacific oysters. Analyses of oyster samples collected during mortality outbreaks demonstrated a significant detection of OsHV-1 (75% of analysed batches), which appeared stronger than previous years. DNA sequencing based on C and IA regions was carried out on 28 batches of OsHV-1 infected Pacific oysters collected in 2008. Polymorphisms were described in both the C and IA regions and characterized a genotype of OsHV-1 not already reported and termed OsHV-1 μVar. A microsatellite zone...
Tipo: Text
Palavras-chave: Herpes virus; Oyster; Crassostrea gigas; OsHV-1 mu Var; Increased mortality; Microsatellites.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12260/9035.pdf

Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication
Provedor de dados: 5
Autores: Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud.
Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters...
Tipo: Text
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf

Herpes-like virus infecting Japanese oyster (Crassostrea gigas) spat
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno.
During the summer of 1993, abnormal sporadic mass mortalities (80-90%) and morbidities occurred among four batches of young Japanese oyster (Crassostrea gigas) spat from the French Atlantic coast. These mortalities were only observed in July. Samples of affected animaIs were examined by electron microscopy. This paper reports the presence and appearance of herpes viruses in these samples.
Tipo: Text
Palavras-chave: Herpes like virus; Mortalities; Crassostrea gigas; Oysters.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-2888.pdf

Tenter de maîtriser les maladies infectieuses chez les mollusques : une clé pour une aquaculture durable
Provedor de dados: 5
Autores: Renault, Tristan.
The world halieutic production reached 133 million tons in 2002. The part of aquaculture in the world halieutic production grew from 25.8% to 29.9% between 1998 and 2002. Over the same period, if the fishing production increased by 6.3%, the aquacultural production progressed by 30%. According to the FAO, the world fish, mollusc and crustacea consumption could be around 179 million tons by 2015. Most of this new demand will have to be satisfied by aquaculture. By 2015, aquaculture could represent 39% of the world halieutic production.  Infectious diseases may influence the survival, but also the growth and zootechnical performances of cultivated animals. As a matter of fact, they are hazards that have to be taken into account and mastered. Aquaculture, as...
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/these-3486.pdf

Etude de l'herpès virus de l'huître. Fonds d'Aide à la Recherche et à l'Innovation. Décision d'Aide à la Recherche n° 95-07-01 (Rapport intermédiaire) 1997
Provedor de dados: 5
Autores: Renault, Tristan.
Le Laboratoire de Génétique, Aquaculture et Pathologie (GAP) de la Station IFREMER de La Tremblade, se propose dans le présent projet d'étudier le virus de type herpès observée chez les huîtres (pouvoir pathogène, conditions d'expression de l'infection, transmission, etc ... ) et de développer des réactifs permettant de réaliser un diagnostic rapide, sensible et fiable de l'infection qu'il occasionne. Les diagnostics proposés seront basés sur la détection directe du virus, soit au moyen de réactifs immunologiques spécifiques des antigènes viraux, soit par des techniques de biologie moléculaire permettant de détecter l'ADN viral (hybridation de sondes nucléiques et réaction PCR : Polymerase Chain Reaction). Ce projet, proposé sur une durée de trois années...
Tipo: Text
Palavras-chave: Virus; Herpès virus; Crassostrea gigas; Pathogènes; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15448/12821.pdf

Compte rendu de la réunion des 14 et 15 Avril 1993 - La Tremblade - Réseau Génétique Mollusques (REGEMO)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Coatanea, Denis; Martin, Anne-genevieve; Barret, Jean; Claude, Serge; Heurtebise, Serge; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Baud, Jean-pierre; Nourry, Max; Bougrier, Serge; Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Le Deuff, Rose-marie; Peignon, Jean-marie.
La discussion générale organisée à la fin de la deuxième journée a fait ressortir les points suivants: 1- Les participants se sont déclarés satisfaits de la tenue de la réunion, tant dans son déroulement, que dans les échanges d'informations auxquels elle a donné lieu. La quantité et la qualité des résultats présentés a permis de mettre en évidence la puissance d'analyse que représente le travail en réseau. 2-L'importance de la valorisation des résultats a été soulignée à plusieurs reprises. La diffusion de l'information devrait se faire dans un premier temps par la voie de rapport internes à la DRV, puis par la voie d'articles publiés dans des revues scientifiques. Sur le programme "C. gigas triploïdes", une première diffusion des résultats obtenus en...
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00049/15981/13423.pdf

Flow cytometry to measure impact of temperature and salinity on the survival of Bonamia ostreae, parasite infecting flat oyster Ostrea edulis, in seawater
Provedor de dados: 5
Autores: Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979&amp;#305; in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely...
Tipo: Text
Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf

Rapport d'activité 1992 de l'Unité de Recherche en Génétique et Ecloserie (URGE) et de l'Unité de Recherche en Pathologie et Immunologie Générales (URPIG)
Provedor de dados: 5
Autores: Gerard, Andre; Renault, Tristan.
Rapport d'activité 1992 de l'Unité de Recherche en Génétique et Ecloserie (URGE) et de l'Unité de Recherche en Pathologie et Immunologie Générales (URPIG) de La Tremblade.
Tipo: Text
Palavras-chave: Génétique; Pathologie; Ecloserie; Immunologie; Invertébrés marins; Rapport activités.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00072/18278/15847.pdf

A herpes-like virus infects a non-ostreid bivalve species: virus replication in Ruditapes philippinarum larvae
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile; Arzul, Isabelle.
Sporadic high mortalities were reported in June 1997 among hatchery-reared larval Manila clam Ruditapes philippinarum in a French commercial hatchery. Cellular abnormalities were observed in semi-thin sections in affected animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in larvae. This is the first description of a herpes-like virus infection in larval R. philippinarum, a non-ostreid bivalve species. Virus particles were similar to other herpes-like viruses described from different oyster species with respect to ultrastructure and morphogenesis. Electron microscopic examination also demonstrated cells with condensed chromatin and extensive perinuclear fragmentation of chromatin. Like viruses infecting oysters,...
Tipo: Text
Palavras-chave: Apoptosis; Virus replication; Mortality; Ruditapes philippinarum; Manila clam; Herpes like virus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-816.pdf

Définition et test de nouvelles amorces pour la détection en PCR du virus de type herpès infectant les huîtres
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile.
La collaboration, entamée en 1996 avec A. Davison du Medical Research Council de Glasgow, a permis d'aboutir à l'obtention d'informations sur la équence de fragments clonés d'ADN de virus de type herpès infectant les huîtres.
Tipo: Text
Palavras-chave: Détection PCR; Virologie; Virus; Herpès; Huîtres creuses; Crassostrea gigas; Techniques.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00199/31048/29465.pdf

Outils de dépistage des pathogènes - Contrat Région Poitou-Charentes 1999 - Convention 99/RPC-A-200 "Pathologie"
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie.
Le Laboratoire de Génétique et Pathologie (LGP) de la station IFREMER de La Tremblade (Charente Maritime) est impliqué aux plans national et international dans l'étude des maladies affectant les mollusques bivalves marins d' intérêt économique et plus particulièrement les huîtres. Des travaux concernant le développement, la validation et l'utilisation d'outils de diagnostic spécifiques d'agents infectieux propres aux coquillages y sont ainsi menés.
Tipo: Text
Palavras-chave: Pathologie; Maladie; Mollusques bivalves; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00043/15471/12850.pdf

Effect of in vivo pesticide exposure and injection of bacteria on immune gene expression in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry.
Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such   contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly  known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and...
Tipo: Text
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf

Introduction d’organismes pathogènes pour les espèces exploitées. Sous-région marine Golfe de Gascogne. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Renault, Tristan; Guichard, Benjamin; Castric, Jeannette.
Tipo: Text
Palavras-chave: Golfe de Gascogne; Organismes pathogènes; Surveillance; Détection; Repamo.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00329/44063/43634.pdf

Histology, cytogenetics and cytofluorymetry in diagnosis of neoplasia in Macoma balthica (Bivalvia, L.) from the southern Baltic Sea
Provedor de dados: 5
Autores: Katarzyna, S; Renault, Tristan; Wolowicz, M.
This study presents results of histopathologic, cytogenetics and flow cytometry analyses performed on Macoma balthica collected from the Gulf of Gdansk (Baltic Sea) in 2003 in order to compare the techniques for diagnosis of neoplasia. The proportion of affected clams gave a crude prevalence of 15.7 %. The four stages of the disease defined by histology and three stages of neoplasia defined by flow cytometry were reported. Stage I defined by flow cytometry corresponded to stages I and II defined by histology. Chromosome analysis did not lead to a staging of neoplastic progression. Both cytogenetics and flow cytometry indicated a difference in the DNA content of non-neoplastic and neoplastic cells. Cytogenetics examination marked that the range of...
Tipo: Text
Palavras-chave: Baltic Sea; Cytofluorymetry; Cytogenetics; Histology; Macoma balthica; Neoplasia.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2921.pdf

Mass mortality in bivalves and the intricate case of the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Barbosa-solomieu, Valerie; Renault, Tristan; Travers, Marie-agnes.
Massive mortality outbreaks in cultured bivalves have been reported worldwide and they have been associated with infection by a range of viral and bacterial pathogens. Due to their economic and social impact, these episodes constitute a particularly sensitive issue in Pacific oyster (Crassostrea gigas) production. Since 2008, mortality outbreaks affecting C. gigas have increased in terms of intensity and geographic distribution. Epidemiologic surveys have lead to the incrimination of pathogens, specifically OsHV-1 and bacteria of the Vibrio genus, in particular Vibrio aestuarianus. Pathogen diversity may partially account for the variability in the outcome of infections. Host factors (age, reproductive status…) including their genetic background that has...
Tipo: Text
Palavras-chave: Pacific oyster; Spat; OsHV-1; Vibrio aestuarianus; Mortality.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00274/38534/37051.pdf

Synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Juin-Septembre 1996
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type . herpès et de la mise au point des méthodes de diagnostic fait suite à la précédente note (fin mai 1996). Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont données et commentés en quatre points. Cette étude est soutenue par la Région Poitou-Charente et la Région Pays de Loire.
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Infection; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15503/12875.pdf

Compte-rendu des résultats d'analyses effectuées en histologie - Année 1995
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno.
Dans le cadre du Réseau zoosanitaires CEE (huître plate) et suivi d'autres espèces, 890 individus provenant des zones 5, 6 et 7 ont été examinés sur l'ensemble de l'année 1995 par différentes techniques : 472 analyses sous coupes histologiques, 328 analyses sur appositions d'organes, 90 analyses sur milieu au thioglycolate.
Tipo: Text
Palavras-chave: Pathologie; Analyse; Histologie; Technique; Bivalves; Ostrea edulis; Huître plate; Mytilus edulis; Ruditapes philippinarum; Ruditapes decussatus; Cerastoderma edule; Mortalité.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00044/15504/12877.pdf

Mortalités massives en élevage larvaire de Lates Calcarifer ( Mise en évidence d'un virus neurotrope )
Provedor de dados: 5
Autores: Renault, Tristan; Thouard, Emmanuel; Weppe, Maurice.
Since 1986 high mortality of hatchery-reared sea bass, Lates Calcarifer, larvae appeared in Ifremer's Pacifie Oceanological Center (Tahiti). Light and electron microscopy show vacuolation and cells with cytoplasmic inclusion bodies in the brain, the cord and the eye relina of affected larvae. Numerous non-enveloped particles identified as viral units, icosahedral in morphology and measuring about 25 nm in diameter were found in the cytoplasm of the degenerating cells. In affected larvae, these particles were isolated by density grandient centrifugations. In vitro tests on cell lines and in vivo test on fish are performed with samples of diseased larvae. And immunisation of mice are made with purified immunoglobulins of Lates calcarifer.
Tipo: Text
Palavras-chave: Aquaculture; Élevage larvaire; Lates calcarifer; Pathologie; Virus; Spongiose; Aquaculture; Hatchery; Lates calcarifer; Pathology; Virus; Spongiosis.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00142/25306/23378.pdf

Etude in vivo et in vitro des effets du mercure sur la réponse hémocytaire de l'huître creuse, Crassostrea gigas : développement de techniques évaluant les impacts de la pollution estuarienne
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
In the last decades, shellfish industry represents an important economic activity in France and particularly in Charente-Maritime. Marennes-Oleron basin is the first French area of oyster production, especially Pacific  oyster, Crassostrea gigas. But this area is also subjected to many pollution due to anthropic activities. Industrial wastes are sources of mercury pollution in the Charente river. The harmful effects of such contaminants on animals inhabiting these estuarine zones are poorly known, although many studies begin to be developed. Most of these studies use bivalve molluscs as biological indicators : these animals can bioaccumulate pollutants within their tissues by filtration. Moreover, they are sedentary so they cannot escape in the case of a...
Tipo: Text
Palavras-chave: Flow cytometry; Haemocytes; Mercury; Crassostrea gigas; Cytométrie de flux; Hémocytes; Mercure; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-830.pdf

Nutrition and reproduction are key parameters in the interaction process leading to Crassostrea gigas oyster summer mortality in France
Provedor de dados: 5
Autores: Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre.
Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence...
Tipo: Text
Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy.
Ano: 2006
URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf

Analysis of Clinical Ostreid Herpesvirus 1 (Malacoherpesviridae) Specimens by Sequencing Amplified Fragments from Three Virus Genome Areas
Provedor de dados: 5
Autores: Renault, Tristan; Moreau, Pierrick; Faury, Nicole; Pepin, Jean-francois; Segarra, Amelie; Webb, Stephen.
Although there are a number of ostreid herpesvirus 1 (OsHV-1) variants, it is expected that the true diversity of this virus will be known only after the analysis of significantly more data. To this end, we analyzed 72 OsHV-1 "specimens" collected mainly in France over an 18-year period, from 1993 to 2010. Additional samples were also collected in Ireland, the United States, China, Japan, and New Zealand. Three virus genome regions (open reading frame 4 [ORF4], ORF35, -36, -37, and -38, and ORF42 and -43) were selected for PCR analysis and sequencing. Although ORF4 appeared to be the most polymorphic genome area, distinguishing several genogroups, ORF35, -36, -37, and -38 and ORF42 and -43 also showed variations useful in grouping subpopulations of this...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00083/19445/17221.pdf

Transcriptomic study of 39 ostreid herpesvirus 1 genes during an experimental infection
Provedor de dados: 5
Autores: Segarra, Amelie; Faury, Nicole; Pepin, Jean-francois; Renault, Tristan.
Massive mortality outbreaks have been reported in France since 2008 among Pacific oysters, Crassostrea gigas, with the detection of a particular OsHV-1 variant called μVar. Virus infection can be induced in healthy spat in experimental conditions allowing to better understand the disease process, including viral gene expression.  Although gene expression of other herpesviruses has been widely studied, we provide the first study following viral gene expression of OsHV-1 over time. In this context, an in vivo transcriptomic study targeting 39 OsHV-1 genes was carried out during an experimental infection of Pacific oyster spat. For the first time, several OsHV-1 mRNAs were detected by real-time PCR at 0 h, 2 h, 4 h, 18 h, 26 h and 42 h post injection. Several...
Tipo: Text
Palavras-chave: Crassostrea gigas; OsHV-1; Viral gene expression; Inhibitors of apoptosis; Real time PCR; Elongation factor.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00184/29549/27875.pdf

Experimental transmission of a Herpes-like virus to axenic larvae of Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Le Deuff, Rose-marie; Nicolas, Jean-louis; Renault, Tristan; Cochennec, Nathalie.
A herpes-like virus which was associated with mortalities amongst hatchery-reared larvae of Pacific oysters, Crassostrea gigas. was observed for the first time in France during the summer 1991 (Nicolas et al.. 1992). Since this first report, we have noted further outbreaks of the disease in the summers of 1992 and 1993 in hatcheries on the western coasts of France in which a similar virus was associated with moribund larvae. Moreover, in the summer 1991, Hine et al. (1992) described a herpes-like virus responsible for larval mortality of hatchery reared C. gigas, in New Zealand. Ail these viruses are c10sely related with respect to their size and morphology and to their apparent cellular tropism for fibroblastic cells.
Tipo: Text
Palavras-chave: Transmission; Herpes like virus; Crassostrea gigas; Oysters.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-2887.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 97 RPC R 58 - Programme Pathologie - Rapport Scientifique année 1997
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf

Histological abnormalities related to mortality in penaeid shrimp (Penaeus stylirostris) reared in New-Caledonia
Provedor de dados: 5
Autores: Berthe, Franck; Legroumellec, Marc; Haffner, Philippe; Renault, Tristan.
Outbreaks of mass mortality occurred since 1993 in penaeid shrimp (Penaeus stylirostris) reared in New-Caledonia. Since then, the disease, called "syndrome 93" (S93) has inflicted serious losses (60 to 100%) during the cold period and thus must be considered as an economically significant disease.
Tipo: Text
Palavras-chave: Penaeus stylirostris; Penaeid shrimp; Mortality; Histology.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2974.pdf

Infection à virus de type herpès chez les larves de coquilles Saint-Jacques
Provedor de dados: 5
Autores: Arzul, Isabelle; Nicolas, Jean-louis; Renault, Tristan.
King scallop (Pecten maximus) larvae suffered massive mortality during the summer of 2000 at a hatchery located in Brittany. Samples were taken for various types of analyses.
Tipo: Text
Palavras-chave: Pathologie; Pecten maximus; Coquilles St Jacques; Herpesvirus; Herpesviridae.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3267.pdf

Programme Herpèsvirus. Synthèse des travaux réalisés au Laboratoire IFREMER de La Tremblade - Mars 1996 - Février 1997
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Ce rapport représente la synthèse des travaux réalisés de mars 1996 à février 1997 au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type herpès observé chez les huîtres et de la mise au point de méthodes de diagnostic. La majorité des travaux rapportés ici de façon complète, a déjà fait l'objet de comptes rendus intermédiaires.
Tipo: Text
Palavras-chave: Pathologie; Herpèsvirus; Herpesviridae; Huître creuse; Crassostrea gigas; Huître plate; Ostrea edulis; Virus; Détection; ¨Protocole; ADN; PCR.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00165/27663/25838.pdf

Etude des agents pathogènes et épidémiologie
Provedor de dados: 5
Autores: Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Robert, Maeva; Chollet, Bruno; Gerard, Andre.
Le programme 4 portant sur la pathologie des coquillages dans le cadre d'une gestion durable des productions ostréicoles, comporte 2 actions principales réparties en une étude d'épidémiologie analytique et descriptive des agents pathogènes (Action 1), et l'étude des relations hôtes/agents pathogènes (Action 2). Les résultats 2001 présentés ici sont relatifs à ces 2 actions, avec notamment des informations sur les infections à virus de type herpès, et de recherche de bactéries pathogènes chez Crassostrea gigas lors des épisodes de mortalités estivales. Par ailleurs, l'émergence d'un programme national, intitulé MOREST (pour Mortalités Estivales) sur l' huître creuse C. gigas va permettre de rappeler le contexte scientifique des relations hôtes/pathogènes.
Tipo: Text
Palavras-chave: Pathologie; Agents pathogènes; Epidémiologie; Huître creuse; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31011/29414.pdf

Introduction d’organismes pathogènes pour les espèces exploitées. Sous-région marine Manche - mer du Nord. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Renault, Tristan; Guichard, Benjamin; Castric, Jeannette.
Tipo: Text
Palavras-chave: Manche; Mer du Nord; Organismes pathogènes; Surveillance; Détection; Repamo.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00328/43876/43450.pdf

First description of French V. tubiashii strains pathogenic to mollusk: I. Characterization of isolates and detection during mortality events
Provedor de dados: 5
Autores: Travers, Marie-agnes; Mersni Achour, Rachida; Haffner, Philippe; Tourbiez, Delphine; Cassone, Anne-laure; Morga, Benjamin; Doghri, Ibtissem; Garcia, Celine; Renault, Tristan; Fruitier-arnaudin, Ingrid; Saulnier, Denis.
Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was...
Tipo: Text
Palavras-chave: Crassostrea gigas Pathogenicity Extracellular products Polyphasic approach; Real time PCR.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00189/30028/28743.pdf

Session de formation à l'utilisation de la technique PCR pour le diagnostic de l'infection à Herpèsvirus chez les huîtres
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile.
Parmi les maladies infectieuses observées chez les mollusques bivalves marins, les viroses sont souvent mal connues, en raison d'une certaine inadéquation des techniques d'identification et de diagnostic généralement mises en oeuvre lors de phénomènes de mortalité. En effet, la microscopie photonique a été et reste encore, dans de nombreux laboratoires travaillant sur les pathologies des mollusques, la technique de base pour l'analyse des échantillons. Cette technique reste insuffisante en cas de pathologie d'origine virale si elle n'est pas complétée par d'autres approches (microscopie électronique à transmission, recherche d'effets cytopathogènes sur monocouches cellulaires et réactifs de diagnostic spécifiques). Aucune lignée de mollusques bivalves...
Tipo: Text
Palavras-chave: Pathologie; Technique laboratoire; PCR; Diagnostic; Herpèsvirus; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15501/12870.pdf

Antiviral immunity in the Pacific oyster, Crassostrea gigas: last development and perspective
Provedor de dados: 5
Autores: Renault, Tristan; Faury, Nicole; Barbosa, Valerie; Moreau, Kevin; Haffner, Philippe; Pepin, Jean-francois.
Herpes-like viruses have been reported last decades in various marine mollusc species in association with mortality outbreaks throughout the world (Renault &amp; Novoa 2004). One of these viruses isolated during French outbreaks has been characterised as an unassigned member of the Herpesviridae family and named ostreid herpes virus 1 (OsHV-1) or Pacific oyster herpes virus (Davison et al. 2005). Studying anti-viral non-specific defence mechanisms (innate immunity) is of great interest, because they constitute the only one in molluscs. Therefore, innate immunity has be investigated in the Pacific cupped oyster, Crassostrea gigas. The main aim of the work was focused on the identification and the characterisation of genes induced by OsHV-1 in the Pacific...
Tipo: Text
Palavras-chave: Pathoogy; Protein gene; Immunity; Herpesviridae; OsHV 1; Crassostrea gigas; Pacific oyster.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4035.pdf

French scallops: A new host for ostreid herpesvirus-1
Provedor de dados: 5
Autores: Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan.
Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been...
Tipo: Text
Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf

Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis
Provedor de dados: 5
Autores: Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek.
A flow cytometry protocol was applied for the detection of neoplasia in Macoma balthica L. from the Gulf of Gdansk (Baltic Sea, Poland). A simple method, based on an osmotic shock, was used to permeabilise gill cells. The cytometric pattern of normal clams consisted of 2 peaks, a major peak B and a smaller peak C. The cytometric pattern of affected clams consisted of 2 peaks named B' and C'. Two parameters were used to define the stages of abnormalities in M. balthica clams based on the percentage of cells in peaks B, C, B' and C' and on the ratio between the fluorescence value of peaks B, C, B' and C' in all individuals. Three stages of neoplasia were clearly distinguished by flow cytometry considering peak C'. Stage 1 was characterised by a major...
Tipo: Text
Palavras-chave: Baltic Sea; Macoma balthica; Neoplasia; Cytogenetics; Histology; Flow cytometry.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2918.pdf

Observations histologiques et analyses en hybridation in situ de bénitiers Tridacna maxima
Provedor de dados: 5
Autores: Arzul, Isabelle; Lapegue, Sylvie; Renault, Tristan.
Tipo: Text
Ano: 2013
URL: https://archimer.ifremer.fr/doc/00156/26683/81049.pdf

Rapport d'activités 2015 de l'Unité SG2M : Santé, Génétique et Microbiologie des Mollusques
Provedor de dados: 5
Autores: Lapegue, Sylvie; Renault, Tristan; Bechemin, Christian; Le Guyader, Soizick; Stavrakakis, Christophe; Rivet, Florence.
L’unité SG2M est structurée pour aborder de manière intégrée les domaines de la santé animale et de la santé humaine liée à production, à la consommation de coquillages, et à la qualité microbiologique du littoral. Ainsi, dans le cadre d’un continuum recherche/surveillance/expertise, et d’une approche pluridisciplinaire, l’unité cherche à développer des connaissances concernant les interactions entre agents infectieux, hôtes et environnement chez les mollusques marins, et les cycles de contamination. Dans le domaine de la recherche, les activités de l’unité sont déclinées selon quatre axes structurants : (1) la microbiologie sanitaire et les voies de transfert des agents pathogènes humains dans l’environnement côtier, (2) l’étude des maladies affectant les...
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00319/43055/42562.pdf

Analysis of hemocyte parameters in Pacific oysters, Crassostrea gigas, reared in the field — Comparison of hatchery diploids and diploids from natural beds
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Soletchnik, Patrick; Faury, Nicole; Kerdudou, Nolwenn; Le Moine, Olivier; Renault, Tristan.
Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster...
Tipo: Text
Palavras-chave: Hatchery; Natural beds; Diploids; Flow cytometry; Peroxidase activity; Esterase activity; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3119.pdf

First evidence of a potential antibacterial activity involving a laccase-type enzyme of the phenoloxidase system in Pacific oyster Crassostrea gigas haemocytes
Provedor de dados: 5
Autores: Luna-acosta, Andrea; Saulnier, Denis; Pommier, Mylene; Haffner, Philippe; De Decker, Sophie; Renault, Tristan; Thomas-guyon, Helene.
Phenoloxidases (POs) are a group of copper proteins including tyrosinase, catecholase and laccase. In several insects and crustaceans, antibacterial substances are produced through the PO cascade, participating in the direct killing of invading microorganisms. However, although POs are widely recognised as an integral part of the invertebrate immune defence system, experimental evidence is lacking that these properties are conserved in molluscs, and more particularly in the Pacific oyster Crassostrea gigas. In the present study, Vibrio splendidus LGP32 and Vibrio aestuarianus 02/041 growths were affected, after being treated with C. gigas haemocyte lysate supernatant (HLS), and either a common substrate of POs, l-3,4-dihydroxyphenylalanine (l-DOPA), to...
Tipo: Text
Palavras-chave: Phenoloxidases; Antibacterial activity; Vibrio; Bivalve; Immunity.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00045/15598/13001.pdf

Genetic parameters of resistance to Vibrio aestuarianus, and OsHV-1 infections in the Pacific oyster, Crassostrea gigas, at three different life stages
Provedor de dados: 5
Autores: Azema, Patrick; Lamy, Jean-baptiste; Boudry, Pierre; Renault, Tristan; Travers, Marie-agnes; Degremont, Lionel.
Background  In France, two main diseases threaten Pacific oyster production. Since 2008, Crassostrea gigas spat have suffered massive losses due to the ostreid herpesvirus OsHV-1, and since 2012, significant mortalities in commercial-size adults have been related to infection by the bacterium Vibrio aestuarianus. The genetic basis for resistance to V. aestuarianus and OsHV-1 and the nature of the genetic correlation between these two traits were investigated by using 20 half-sib sire families, each containing two full-sib families. For each disease, controlled infectious challenges were conducted using naïve oysters that were 3 to 26 months old. In addition, siblings were tested under field, pond and raceway conditions to determine whether laboratory...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00372/48350/48518.pdf

Introduction d’organismes pathogènes pour les espèces exploitées. Sous-région marine Méditerranée occidentale. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Renault, Tristan; Guichard, Benjamin.
Tipo: Text
Palavras-chave: Méditerranée; Organismes pathogènes; Surveillance; Détection; Repamo.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00331/44246/80281.pdf

Marteilia in American oysters Crassostrea virginica reared in France
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno.
Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared at 3 different locations. A survey was taken of these young C. virginica oysters during 1993. Histological and electron microscopical examination revealed a parasite interpreted as a Martelia-like protozoan in 1 oyster. The structure and the initial stages of the developmental cycle of this parasite are described for the first time in American oysters. However, no mature spores were detected. Thus, exact identification of the protozoan is difficult. This study indicates that American oysters could be infected with Marteilia spp. during cohabitation with Marteilia-infected native bivalves in France.
Tipo: Text
Palavras-chave: Marteilia like parasite; Marteiliosis; Crassostrea virginica; American oyster.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/publication-2897.pdf

Comparison of Heamograms from resistant and susceptible European flat oysters, Ostrea edulis, exposed to the parasite, Bonamia ostreae
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Chollet, Bruno; Maffart, Patricia.
To study the cellular defence mechanisms involved in the resistance of the flat oysters against Bonamia ostreae, a comparaison of haemograms was performed between susceptible and resistant animals
Tipo: Text
Palavras-chave: Pathogen; Bonamia ostreae; Ostrea edulis.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2906.pdf

Herpesvirus infection in European flat oysters Ostrea edulis obtained from brood stocks of various geographic origins and grown in Galicia (NW Spain)
Provedor de dados: 5
Autores: Da Silva, P; Renault, Tristan; Fuentes, J; Villalba, A.
We evaluated differences in productive traits and disease susceptibility among Ostrea edulis stocks. We produced 4 to 5 families from each of 4 oyster populations (Irish, Greek and 2 Galician) in a hatchery. Spat corresponding to 19 different families were transferred to a raft in the Ria de Arousa (Galicia, Spain) for grow-out. Samples of each family were histologically processed every month for 2 yr. One of the pathological conditions disclosed by histological examination was characterised by the occurrence of numerous abnormal cells throughout the connective tissue of various organs, showing hypertrophied nuclei with marginated chromatin and a characteristic large intranuclear acidophilic inclusion. Ultrastructural examination showed that the abnormal...
Tipo: Text
Palavras-chave: In situ hybridisation; Transmission electron microscopy; Histology; Bonamiosis; Ostreid herspesvirus 1; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3735.pdf

Recherche d'AND d'herpèsvirus infectant les bivalves dans des échantillons d'eau de claires ostréicoles
Provedor de dados: 5
Autores: Solliec, Gaëlle; Vigneron, Valérie; Montanie, Hélène; Renault, Tristan.
Viral particles, associated with high mortality rates, have been reported among larvae and juveniles in different marine bivalve specie and characterized as member of the Hespesviridae family. Most studies focused on the detection of such viral infections among economically important species. However, the persistence of bivalve herpes viruses in marine environment is poorly documented. This topic appears as an important research domain in order to clarify the natural infection cycle of the herpesvirus of marine bivalves.
Tipo: Text
Palavras-chave: Herpesviridae; Oyster; PCR; DNA; Herpesvirus; Huître; PCR; ADN.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2856.pdf

Effects of temperature and salinity on haemocyte activities of the Pacific oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Frouin, Heloise; Moreau, Kevin; Thomas Guyon, Helene; Renault, Tristan.
The Pacific oyster, Crassostrea gigas, is extensively cultivated and represents an important economic activity. Oysters are reared in estuarine areas, subjected to various biotic and abiotic factors. One of the limiting factors in aquaculture is mortality outbreaks, which may limit oyster production, and the causes of these outbreaks are not completely understood. In this context, the effects of temperature and salinity on Pacific oyster, C. gigas, haemocytes, were studied. Haemocytes are the invertebrate blood cells and thus have been shown to be involved in defence mechanisms. Flow cytometry was used for monitoring several haemocyte parameters. An increase of temperature induced an increase of haemocyte mortality, in both in vitro and in vivo...
Tipo: Text
Palavras-chave: Cellular activity; Flow cytometry; Salinity; Temperature; Haemocyte; Crassostrea gigas; Pacific oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-934.pdf

Diagnosis of herpes-like virus infections in oysters using molecular techniques
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile.
Several herpes-like virus infections have been reported since 1991 among different bivalve species around the world (Nicolas et al., 1992; Comps and Cochennec, 1993; Renault et al., 1994; Hine and Thome, 1997; Hine 1997). To date, detection of these infections has been strongly correlated with severe losses of oyster larvae and spat. The virus infections are currently diagnosed by laborious histologicai and transmission electron microscopical examinations. These techniques, however, facilitate neither rapid diagnosis nor the ability to analyse large sample numbers.
Tipo: Text
Palavras-chave: Diagnosis; Ostrea edulis; Crassostrea gigas; Herpes like virus.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/acte-2902.pdf

Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers
Provedor de dados: 5
Autores: Hubert, Francoise; Morga, Benjamin; Renault, Tristan; Le Guyader, Soizick.
Aims  This study was performed to develop passive sampling methodology for the detection of two viruses in seawater in the area of shellfish production, The Norovirus (NoV), a human pathogen implicated in gastroenteritis outbreaks linked to oyster consumption and the ostreid herpesvirus type 1 (OsHV-1) a virus associated with mass mortalities of Pacific oysters. Methods and Results  Commercially membranes were tested for their capacity to adsorb virus: Zetapor, gauze, nylon, low density polyethylene (LDPE) and Polyvinylidene difluoride (PVDF). Laboratory exposures of membranes to contaminated water samples (stool, sewage, seawater) were performed. Our data shown that the amount of NoV GII genome per membrane measured with qRT-PCR increased with the time of...
Tipo: Text
Palavras-chave: Norovirus; OsHV-1; Pacific oysters; Passive samplers; Seawater; Sewage.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00365/47646/47670.pdf

A unique self-organization of bacterial sub-communities creates iridescence in Cellulophaga lytica colony biofilms
Provedor de dados: 5
Autores: Kientz, Betty; Luke, Stephen; Vukusic, Peter; Peteri, Renaud; Beaudry, Cyrille; Renault, Tristan; Simon, David; Mignot, Tam; Rosenfeld, Eric.
Iridescent color appearances are widespread in nature. They arise from the interaction of light with micron- and submicron-sized physical structures spatially arranged with periodic geometry and are usually associated with bright angle-dependent hues. Iridescence has been reported for many animals and marine organisms. However, iridescence has not been well studied in bacteria. Recently, we reported a brilliant “pointillistic” iridescence in colony biofilms of marine Flavobacteria that exhibit gliding motility. The mechanism of their iridescence is unknown. Here, using a multi-disciplinary approach, we show that the cause of iridescence is a unique periodicity of the cell population in the colony biofilm. Cells are arranged together to form hexagonal...
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00311/42188/41488.pdf

Cellular and molecular responses of haemocytes from Ostrea edulis during in vitro infection by the parasite Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Chollet, Bruno; Arzul, Isabelle.
Bonamia ostreae is a protozoan, affiliated to the order Haplosporidia and to the phylum Cercozoa. This parasite is intracellular and infects haemocytes, cells notably involved in oyster defence mechanisms. Bonamiosis due to the parasite B. ostreae is a disease affecting the flat oyster, Ostrea edulis. The strategies used by protozoan parasites to circumvent host defence mechanisms remain largely unknown in marine bivalve molluscs. In the present work, in vitro experiments were carried out in order to study the interactions between haemocytes from O. edulis and purified parasite. B. ostreae. We monitored cellular and molecular responses of oyster haemocytes by light microscopy, flow cytometry and real-time PCR 1, 2, 4 and 8 h p.i. Light microscopy was used...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Haemocytes; Real-time PCR; Flow cytometry; Super oxide dismutase.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00037/14874/18001.pdf

Réseau de veille zoosanitaire des mollusques REPAMO - Rapport d'activité pour l'année 1997
Provedor de dados: 5
Autores: Berthe, Franck; Pichot, Yves; Chollet, Bruno; Thebault, Anne; Kuntz, Gregoire; Tige, Gilbert; Thebault, Anne; Fleury, Pierre-gildas; Hirata, Toshio; Le Coguic, Yvette; Renault, Tristan; Lipart, Cecile; Grizel, Henri; Joly, Jean-pierre; Mazurie, Joseph; Baud, Jean-pierre; Goulletquer, Philippe; Dreno, Jean-paul; Buestel, Dominique.
Le réseau de veille zoosanitaire des mollusques, REPAMO, a été créé en 1992 afin de protéger les espèces d'intérêt économique des maladies susceptibles de les affecter. En effet, de même que les autres productions animales, la conchyliculture est frappée par diverses maladies dont certaines se comportent comme des facteurs limitants de la qualité ou de la quantité des productions. L' histoire de l'ostréiculture française a connu de tristes expériences en la matière, notamment dans les années 70 pour l' huître creuse portugaise, Crassostrea angulata ou encore pour l'huître plate, Ostrea edulis. En fait, nous ne disposons que de très peu de possibilités de protéger les mollusques bivalves vis à vis des maladies infectieuses. D' une part, les traitements,...
Tipo: Text
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00098/20901/18521.pdf

Bilans depuis 1999 et évolution de la stratégie du REPAMO
Provedor de dados: 5
Autores: Thebault, Anne; Martin, Anne-genevieve; Pichot, Yves; Chollet, Bruno; Robert, Maeva; Le Coguic, Yvette; Tige, Gilbert; Hirata, Toshio; Mazurie, Joseph; Le Saux, Jean-claude; Masson, Nadine; Auby, Isabelle; Maurer, Daniele; Le Roux, Frédérique; Cochennec, Nathalie; Renault, Tristan; Berthe, Franck; Grizel, Henri.
The main objectives of the Mollusc Pathology Network (REPAMO) are to prevent introductions or outbreaks of infectious agents, prevent spread within a farm and especially from one farm to another, to study ways of decreasing the impact of infectious agent and to monitor the development of these pathogens in order to guarantee the health status of oysters exchanged with our commercial partners. On the whole, REPAMO's activities have not changed and include: - monitoring the development of diseases that have to be reported to authorities and carrying out analyses to classify zones: bonamiosis and marteiliosis; - baseline monitoring of farmed and wild populations of bivalve molluscs; - studying cases of abnormal mortality events; - controlling mollusc...
Tipo: Text
Palavras-chave: Mollusques; Réseau surveillance; Pathologie; REPAMO.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3265.pdf

Identifying the viral genes encoding envelope glycoproteins for differentiation of Cyprinid herpesvirus 3 isolates
Provedor de dados: 5
Autores: Han,; Kim, Ji Hyung; Renault, Tristan; Choresca, Casiano, Jr.; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang.
Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA,...
Tipo: Text
Palavras-chave: Cyprinid herpes virus 3; Glycoprotein gene; Koi herpesvirus; Microsatellite; Polymorphism; Phylogenetic tree.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00118/22940/20768.pdf

Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast
Provedor de dados: 5
Autores: Renault, Tristan; Stokes, Nancy; Chollet, Bruno; Cochennec, Nathalie; Berthe, Franck; Gerard, Andre; Burreson, Eugene.
Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A...
Tipo: Text
Palavras-chave: Haplosporidium nelsoni; Haplosporidiosis; Crassostrea gigas; Pacific oyster.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-821.pdf

Caractérisation des facteurs de risques associés au mortalités estivales - Synthèse du thème 3 - Risque associé au stress environnemental - 2002-2005
Provedor de dados: 5
Autores: Burgeot, Thierry; Gagnaire, Beatrice; Renault, Tristan; Haure, Joel; Moraga, D.; David, E.; Boutet, L.; Sauriau, P.g.; Malet, Nathalie; Bouchet, V.; Lapegue, Sylvie; Bouilly, Karine; Le Moullac, Gilles; Arzul, Genevieve; Knoery, Joel; Quiniou, Francoise; Bacher, Cedric; Soletchnik, Patrick.
Les mortalités d'huîtres observées sur les côtes françaises apparaissent principalement à proximité du sédiment et pendant la gamétogenèse. Les interactions de facteurs environnementaux sur les écosystèmes conchylicoles comme la température, les apports des bassins versants et le compartiment sédimentaire illustrent un risque de mortalité lié à différentes sources de stress (Thème 1). Les variations de la qualité de la ressource trophique pendant l'effort de gamétogenèse favorise une baisse de l'énergie disponible (Thème 2) et contraint l'organisme fragilisé à fournir un effort d'adaptation (Fent 2004). L'apparition d'un stress pendant cette période sensible des huîtres pourrait constituer un facteur aggravant de mortalité. L'objectif de cette étude est...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00304/41538/40746.pdf

Phylogenetic and morphological characterisation of the green algae infecting blue mussel Mytilus edulis in the North and South Atlantic oceans
Provedor de dados: 5
Autores: Rodriguez, Francisco; Feist, Stephen; Guillou, Laure; Harkestad, Lisbeth; Bateman, Kelly; Renault, Tristan; Mortensen, Stein.
Blue mussels Mytilus edulis with shell deformations and green pustules containing parasitic algae were collected at 3 coastal sites (Burøy, Norway; Bockholm, Denmark; Goose Green, Falkland Islands). A comparative study, including mussel histopathology, algal morphology, ultrastructure and phylogenetic position was performed. Green pustules were mainly located in the posterior portion of the mantle and gonad tissues and the posterior adductor muscle. Electron microscopy confirmed the presence of algal cells with similar morphology to Coccomyxa parasitica. Algae were oval shaped with a single nucleus and chloroplast, 1 or 2 mitochondria and a dense granular cytoplasm with a lipid inclusion body, Golgi apparatus and small vesicles. Partial small subunit (SSU)...
Tipo: Text
Palavras-chave: Phylogeny; Parasitic alga; Mytilus edulis chilensis; Mytilus edulis; Coccomyxa parasitica; Blue mussels.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4581.pdf

Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae
Provedor de dados: 5
Autores: Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle.
The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses....
Tipo: Text
Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf

Effects of temperature and salinity on the survival of Bonamia ostreae, a parasite infecting flat oysters Ostrea edulis
Provedor de dados: 5
Autores: Arzul, Isabelle; Gagnaire, Beatrice; Bond, Celine; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster Ostrea edulis. The parasite has been described in various ecosystems from estuaries to open sea, but no clear correlation has yet been demonstrated between disease development and environmental parameters. In this study, the effect of temperature and salinity on the survival of purified parasites maintained in vitro in seawater was investigated by flow cytometry. Purified parasites were incubated in various seawater media (artificial seawater, natural seawater, seabed borewater) at various temperatures (4, 15 and 25°C) and subjected to a range of salinities from 5 to 45 g l–1. Parasites were collected after 12, 24 and 48 h of...
Tipo: Text
Palavras-chave: Salinity tolerance; Temperature tolerance; Cell viability; In vitro assays; Flow cytometry; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6443.pdf

Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf

Epidémiologie analytique et descriptive des agents pathogènes, étude de la diversité des virus de type herpès (action 1). Etude des relations hôtes/agents pathogènes, pathogénèse des infections à virus de type herpèsvirus (action 2) - Contrat Région Poitou-Charentes 2000-2006
Provedor de dados: 5
Autores: Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno.
Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés.
Tipo: Text
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf

Detection of Bonamia ostreae and B. exitiosa (Haplosporidia) in Ostrea edulis from the Adriatic Sea (Italy)
Provedor de dados: 5
Autores: Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G.
The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,...
Tipo: Text
Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf