Consulta: creatorFacets:"Robert, Rene"
Registros recuperados: 102
Data/hora: 11/06/2024 08:10:05

Approche économique de la pêche bonitière en Polynésie française
Provedor de dados: 5
Autores: Ugolini, Bruno; Robert, Rene; Mauri, Moea.
Tipo: Text
Ano: 1983
URL: http://archimer.ifremer.fr/doc/00091/20202/17864.pdf

Testing of the Argentina oyster, Ostrea puelchana, in several French oyster farming sites
Provedor de dados: 5
Autores: Pascual, Marcela; Martin, Anne-genevieve; Zampatti, Eduardo; Coatanea, Denis; Defossez, Jérôme; Robert, Rene.
Because of diseases affecting fIat oyster Ostrea edulis, acclimatization of the Argentina oyster Ostrea puelchana has been tested in 1989-90 in five French oyster farming sites. Following ICES recommendations , hatchery produced juveniles, from parents imported from Argentina, were transferred to experimental si tes in summer 1989. Very high mortalities occurred, reaching 46 to 98% in March-Apri1 1990. They continued later on, resulting in the end of experiments in March 1991. The growth of surviving oysters was relatively poor in comparison of the local species. Parasites Marteilia refringens and Bonamia ostreae have been detected but at rates inadequate ta explain the high mortalities. AlI these results lead not to retain this species for breeding on...
Tipo: Text
Palavras-chave: Huître; Ostrea puelchana; Parasitologie; Marteilia refringens; Oysters; Ostrea puelchana; Parasitology; Marteilia refringens.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00018/12946/9916.pdf

Influence of food density and temperature on ingestion, growth and settlement of Pacific oyster larvae, Crassostrea gigas
Provedor de dados: 5
Autores: Rico Villa, Benjamin; Pouvreau, Stephane; Robert, Rene.
Ingestion, growth and metamorphosis of Pacific oyster, Crassostrea gigas, larvae were studied under controlled conditions of food density and temperature using a combination of a flow-through rearing system and a hydrobiological monitoring device. In a first experiment larvae were exposed to three different phytoplankton densities (12, 20 and 40 cells &amp;#956;l-1) while in a second trial larvae were reared at five different temperatures (17, 22, 25, 27 and 32°C). Both food concentration and temperature significantly affected the larval physiology throughout the entire development from D-veliger to young spat. Larvae survived over a wide range of both environmental parameters with high survival at the end of experiments. The feeding functional response...
Tipo: Text
Palavras-chave: Temperature; Food; Growth; Ecophysiology; Bivalve larvae; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2008/publication-4747.pdf

Effects of blue light on the biochemical composition and photosynthetic activity of Isochrysis sp. (T-iso)
Provedor de dados: 5
Autores: Marchetti, Julie; Bougaran, Gael; Jauffrais, Thierry; Lefebvre, Sebastien; Rouxel, Catherine; Saint-jean, Bruno; Lukomska, Ewa; Robert, Rene; Cadoret, Jean-paul.
In aquaculture, particularly in bivalve hatcheries, the biochemical composition of algal diets has a strong influence on larval and post-larval development. Biochemical composition is known to be related to culture conditions, among which light represents a major source of variation. The effects of blue light on biochemical composition and photosynthetic rate of Isochrysis sp. (T-iso) CCAP 927/14 were assessed in chemostat at a single irradiance (300 μmol photons m−2 s−1) and compared with white light. Two different dilution (renewal) rates were also tested: 0.7 and 0.2 d−1. Relative carbohydrate content was lower under blue light than under white light at both dilution rates, whereas chlorophyll a and photosynthesis activity were higher. In contrast,...
Tipo: Text
Palavras-chave: Isochrysis; Blue light; Photosynthesis; Proximate composition; Chemostat.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00085/19599/17237.pdf

Compte-rendu de mission en Tunisie (13-17 Juin 2005). Atelier de travail sur les nouvelles perspectives de la recherche scientifique en aquaculture
Provedor de dados: 5
Autores: Thouard, Emmanuel; Buchet, Vincent; Cahu, Chantal; Coeurdacier, Jean-luc; Elie, Pierre; Gerard, André; Hussenot, Jerome; Legendre, Marc; Prou, Jean; Robert, Rene.
Les missions réalisées fin 2004 et début 2005 ont permis de faire le point sur les coopérations potentielles entre l'INSTM et l'IFREMER. Un certain nombre de domaines de coopération et de sujets de collaboration ont été identifiés et se sont traduits (ou se traduiront) par des actions de coopération (missions, stages, formations) soutenues financièrement par le SCAC de l'Ambassade de France à Tunis. La coopération entre l'IFREMER et l'INSTM dans le domaine de l'aquaculture a été très active dans le cadre du programme« Aquaculture 2001 » (1997-2001). Depuis cette période les relations se sont limitées à la préparation du programme DORAD qui était la suite d' «Aquaculture 2001 » . En l'absence du financement FSP sollicité pour DORAD, les deux organismes ont...
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00305/41648/40857.pdf

Eléments de biologie larvaire et postlarvaire de mollusques bivalves en milieu naturel et conditions contrôlées
Provedor de dados: 5
Autores: Robert, Rene.
Tipo: Text
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00090/20148/17798.pdf

Projet CRYOYSTER: Optimisation, standardisation et validation de la congélation de laitance d'huître creuse Crassostrea gigas à des fins de conservation et de diffusion génétique - Appel d'offre de l'OFIMER du 8 Novembre 2001 - Rapport final
Provedor de dados: 5
Autores: Brizard, Raphael; Bernardi, Maud; Boudry, Pierre; Haffray, Pierrick; Labbe, Catherine; Maisse, Gérard; Maurouard, Elise; Robert, Rene; Roger, Jean-luc.
Afin d'appuyer la filière ostréicole, les programmes de recherche actuels visent à proposer des méthodes d'amélioration génétique des rendements et de la qualité du produit (sélection, polyploïdisation). A l'image des productions animales terrestres, la continuation de ces travaux et la diffusion du progrès génétique dans la filière seraient grandement facilitée par le développement de techniques de conservation des génotypes sélectionnés. La cryopréservation qui est utilisée fréquemment dans les programmes de sélection des animaux terrestres et des espèces piscicoles n'est toujours pas disponible pour les mollusques bivalves. Cette technique présente les atouts qui peuvent pourraient satisfaire les différents acteurs de la filière ostréicole car elle...
Tipo: Text
Palavras-chave: Congélation sperme; Standardisation; Procédure; Ecloserie expérimentale; Fécondation; Cryopréservation; Sperme cryopréservé.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00049/16056/13516.pdf

A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae
Provedor de dados: 5
Autores: Rico Villa, Benjamin; Woerther, Patrice; Mingant, Christian; Le Piver, David; Pouvreau, Stephane; Hamon, Michel; Robert, Rene.
While literature is relatively abundant on adult shellfish, ecophysiological studies at the larval stage are scarce because of both technical difficulties and inadequate methodology. A tool dedicated to provide basic information for larval ecophysiology was accordingly developed. Two steps were followed: first a flow-through method of Crassostrea gigas larval culture was perfected during a set of experiments in which rearing systems and larval density were assessed. Then a continuous hydrobiological data recorder was adapted with modifications to comply with our experimental aim. SILO (Système d'Instrumentation des Larves en flux Ouvert) allowed the successive acquisition of hydrobiological parameters in ten 150 l larval tanks in which larvae were reared...
Tipo: Text
Palavras-chave: Temperature; Ecophysiology; Bivalve larvae; Crassostrea gigas; Flow through.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4167.pdf

Utilisation des "algues-fourrage" en aquaculture
Provedor de dados: 5
Autores: Chretiennot-dinet, Marie-josèphe; Robert, Rene; His, Edouard.
Studies concerned with unicellular algae as a food source for laval and post-larval stages of commercially valuable bivalves are reviewed. As only about ten species of microalgae are now produced in large quantities in commercial hatcheries, these are described and their main characteristics illustrated. Finally the properties which make tem a suitable source of food for rearing bivalves are discussed.
Tipo: Text
Ano: 1986
URL: http://archimer.ifremer.fr/doc/00021/13251/10293.pdf

Utilisation des élevages larvaires de Crassostrea gigas en écotoxicologie marine
Provedor de dados: 5
Autores: His, Edouard; Robert, Rene.
The study of the anomalies occurring in the reproduction of Crassostrea gigas in the Arcachon Bay has been addressed through the development of some experimental protocols aimed at highlighting the action of a number of alteragenes on veligeres. The techniques used are described. They are based on the research of the action limits of the products tested on the breeding, formation and growth of oyster larvae. In addition, the sensitivity to micropollutants of the algae the veligere feed on is precised. Finally, larviculture and algae growth monitoring enabled the assessment of the quality of the sea-water and of the sediments in shellfish farming areas.
Tipo: Text
Palavras-chave: Chaetoceros calcitrans; Isochysis galbana; Algues unicellulaires; Crassostrea gigas; Larves; Bio essais; Ecotoxicologie.
Ano: 1985
URL: http://archimer.ifremer.fr/doc/1985/acte-6184.PDF

Essai de culture de l'huître plate Ostrea edulis dans le bassin d'Arcachon. Résultats préliminaires
Provedor de dados: 5
Autores: Robert, Rene; Pichot, Yves; Comps, Michel.
From 1988 to 1989, a study has been conducted pertaining to the growth of the flat oyster Ostrea edulis, as well as Marteilia refringens and Bonamia ostreae outbreaks.  Commercial mono specific farming (flat oysters), with low density or mixed (flat and hollow oysters) along with higher density, has been taking place in the Arcachon basin oceanographic sectors, where main hydrobiological parameters were acquired simultaneously.   The excellent growth of the flat oyster was witnessed in mono specific farming. However this growth was average in mixed farming, probably due to higher total oyster density and interspecies competition. Regardless of the type of farming, Marteilia refringens infection was very quick and may have been related to climatic bay water...
Tipo: Text
Palavras-chave: Basin of Arcachon; Pathology; Bonamia ostreae; Marteilia refringens; Growth; Ostrea edulis; Bassin d'Arcachon; Pathologie; Bonamia ostreae; Marteilia refringens; Croissance; Ostrea edulis.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/rapport-1592.pdf

Influence of diet assemblage on Ostrea edulis broodstock conditioning and subsequent larval development
Provedor de dados: 5
Autores: Gonzalez, Ricardo; Mingant, Christian; Petton, Bruno; Robert, Rene.
In contrast with the Japanese oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum, Ostrea edulis seed production in the hatchery has been reported to be erratic, with sudden and unexplained larval and post-metamorphosis mortalities. Fecundity and initial larval quality have been related to broodstock conditioning, but effects on larval development and metamorphosis remain poorly understood. In addition, molluscan larval mortalities have been often associated with bacterial contamination and flow-through techniques may help to overcome this problem. Both aspects have been considered in the present work. O. edulis broodstock were conditioned at 19 °C and fed three different microalgal diets. Two were single-species diets: Rhodomonas salina...
Tipo: Text
Palavras-chave: Ostrea edulis; Feeding; Broodstock conditioning; Larval development; Flow-through.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00090/20093/17732.pdf

Etudes sur les causes de la perturbation de la reproduction et du développement larvaire de Crassostrea gigas dans le bassin d'Arcachon
Provedor de dados: 5
Autores: Robert, Rene.
Centre autonome de production, d'élevage et de commercialisation, le bassin d'Arcachon assurait ces dernières années une part non négligeable de l'approvisionnement en naissain d'huîtres creuses des régions conchylicoles du littoral septentrional et méditerranéen de la France.
Tipo: Text
Ano: 1983
URL: http://archimer.ifremer.fr/doc/00090/20149/17799.pdf

Mise au point d'outils de gestion des gamètes mâles d'huîtres creuses Crassostrea gigas diploïdes et tétraploïdes à des fins de conservation et de diffusion
Provedor de dados: 5
Autores: Brizard, Raphael; Boudry, Pierre; Haffray, Patrick; Labbe, C.; Suquet, Marc; Robert, Rene; Masse, G.; Pepin, Jean-francois; Saulnier, Denis.
In order to improve oyster culture, many research programs try and propose new methods aiming at improving genetically the product performances and quality (selection, polyploidisation). As for terrestrial animal production, the continuity of the different works as well as the spreading of the genetical progress would be made easier by the development of conservation techniques of the selected genotypes. Cryopreservation, which is often used in selection programs of terrestrial animals or fish farming species, is not always available for bivalves. This technique would enable the conservation of the gametes of individuals selected for their high growth potential, their resistance to diseases and/or their quality for a smaller cost and less risks than the...
Tipo: Text
Palavras-chave: Tetraploïde; Diploïde; Concervation; Spermatozoides; Cryoconservation; Génétique; Crassostrea gigas; Huîtres.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3339.pdf

The food quality of Tetraselmis suecica slurry for Crassostrea gigas spat
Provedor de dados: 5
Autores: Robert, Rene; Parisi, Guilina; Pastorelli, Roberta; Poli, Bianca Maria; Tredici, Mario.
In an attempt to provide cost-effective alternatives to live microalgae and to simplify hatchery-nursery procedures, concentrated microalgae appear to be very promising. Due to its high preservation ability, Prasinophytes appear to be good candidates for such alternative diets. Nevertheless, in a previous study the utilization of Tetraselmis suecica was only conceivable for old Crassostrea gigas larvae. In the present work its nutritional quality has been accordingly searched on ]apanese oyster's juveniles. Whatever the assemblages and condition, fresh or preserved microalgae, T. suecica did not allow an adequate postlarval development in C. gigas.
Tipo: Text
Palavras-chave: Nutritional quality; Crassostrea gigas; Tetraselmis suecica; Microalgae; Valeur alimentaire; Crassostrea gigas; Tetraselmis suecica; Microalgues.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-6202.PDF

Dispositifs de concentration de poissons en Polynésie française
Provedor de dados: 5
Autores: Ugolini, Bruno; Robert, Rene.
Tipo: Text
Ano: 1982
URL: http://archimer.ifremer.fr/doc/00091/20201/17863.pdf

Manila clam Tapes philippinarum culture: Sediment-clam interactions
Provedor de dados: 5
Autores: Goulletquer, Philippe; Robert, Rene; Trut, Gilles.
Manila clam (Tapes philippinarum) culture and sediment interactions were tested by comparing two rearing areas, including an oceanic ('Le-Ferret') and a more estuarine ('Les-Jacquets') sites in the bay of Arcachon (France). The growth of a calibrated clam population (10-mm spat) was monitored in these two areas with a concomitant sediment-water interface survey over a 1.5-year period. Two sites per area, including control and rearing plots, were sampled on a monthly basis. The potential clam farming impacts by bioturbation and interactions were examined at three sediment depths: 0-1, 1-2 and 2-10 cm. Moreover, the main hydrobiological parameters were measured on a weekly basis to establish a relationship between these parameters and sediment-water...
Tipo: Text
Palavras-chave: Bay of Arcachon; Tapes philippinarum; Sediment water interface; Bioturbation; Clam culture; Bassin d'Arcachon; Tapes philippinarum; Interface eau sédiment; Bioturbation; Vénériculture.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-864.pdf

Impact des facteurs anthropiques sur le recrutement de l'huître : l'exemple du bassin d'Arcachon
Provedor de dados: 5
Autores: His, Edouard; Robert, Rene.
"Il est impossible d'imaginer un lieu plus favorable à l'ostréiculture que le bassin d'Arcachon" (Yonge, 1950). En effet, l'huître indigène, Ostrea edulis, et l'espèce plus récemment introduite, Crassostrea gigas, peuvent s'y reproduire en abondant. Si l'exploitation de la première a pris fin à la suite de deux épizooties consécutives, la seconde est l'objet d'une culture intensive dont la production annuelle est d'environ 12 000 tonnes de mollusques commercialisés. Des anomalies ont sévi dans la baie, de 1977 à 1981, rendant les récoltes de naissain pratiquement sensibles aux facteurs d'agression, et ont même été proposées par Woelke (1967) comme matériel biologique, afin de tester la qualité des eaux en zone littorale. La technique des élevages larvaires...
Tipo: Text
Palavras-chave: Bassin d'Arcachon; Crassostrea gigas; Larves; Température; Nutrition; Bioessais; Facteurs anthropiques.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00019/13055/10013.pdf

Substitutes for live microalgae in mariculture: a review
Provedor de dados: 5
Autores: Robert, Rene; Trintignac, P.
In mollusc hatcheries and nurseries, live microalgae are traditionally used as feed for bivalves. Despite recent improvements in efficiency, such intensive culture of microalgae represents major cost factors in the production of bivalve larvae and spat. In an attempt to provide cost-effective alternatives and to simplify hatchery-nursery procedures, non-living algal food has been developed over the past few years. Substitution products such as bacteria, yeast, vitamin or docosahexaenoic acid enriched powders, dry and concentrated microalgae and microcapsules have been tested and evaluated on an experimental scale. The present article reviews major research aimed at developing non living diets in mariculture, which generally resulted in lower growth and...
Tipo: Text
Palavras-chave: Mollusc rearing; Artificial feed; Microalgae; Hatchery; Nursery.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00090/20076/17719.pdf

Optimisation de la production en écloserie de mollusques : Mise au point d'un pilote industriel de production phytoplanctonique en continu et des techniques associées pour leur intégration en écloserie commerciale
Provedor de dados: 5
Autores: Angeri, S.; Bougaran, Gael; Connan, Jean-paul; Jouin, Jean-claude; Kaas, Raymond; Le Dean, Loic; Legrand, J.; Loubiere, K.; Lukomska, Ewa; Olivo, Erell; Picquet, Marie-lise; Pruvost, J.; Robert, Rene.
Les travaux présentés dans le cadre de ce rapport d'activité rendent compte des avancées réalisées sur le plan (1) biologique, avec d'une part une étude sur le comportement du genre Chaetoceros en culture continue et, d'autre part, les résultats d'expérimentations sur les performances d'élevages larvaires réalisés sur la base de cultures de microalgues en continu, et (2) technologique, avec la continuité des actions de développement du photobioréacteur destiné aux écloseries commerciales ainsi que des périphériques indispensables à son intégration dans le secteur aquacole.
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00304/41518/40726.pdf

Anaesthesia and gonad sampling in the European flat oyster (Ostrea edulis)
Provedor de dados: 5
Autores: Suquet, Marc; Gonzalez, Ricardo; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Robert, Rene.
Controlled reproduction of the European flat oyster requires the development of tools adapted to this species, including a practical anaesthesia and gonad sampling protocol to facilitate sex determination and the verification of gametogenesis. Three replicate groups of 10 oysters (mean weight +/- SD: 29.9 +/- 8.5 g) were anaesthetised in 5 L containers using magnesium chloride designed either for laboratory (Flucka (R)) or agricultural (Dead Sea Work (R): DSW) use, at two concentrations (50 or 72 g L-1). No significant differences were observed in the percentages of oysters anaesthetised or subsequent oyster mortality with the different anaesthetics or concentrations, but increasing water temperature from 14.9 to 18.8 degrees C significantly increased the...
Tipo: Text
Palavras-chave: Oyster; Ostrea edulis; Anaesthesia; Magnesium chloride; Gonad sampling.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00018/12907/9876.pdf

A glimpse on the mollusc industry in Europe
Provedor de dados: 5
Autores: Robert, Rene; Sanchez, Jose Luis; Perez-paralle, Luz; Ponis, Emanuele; Kamermans, Pauline; O'Mahoney, Maria.
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00126/23737/21744.pdf

Croissance de l'huitre japonaise Crassostrea gigas en cylindre rotatif Stanway. Premiers resultats en France
Provedor de dados: 5
Autores: Robert, Rene; Maurer, Daniele.
A new method of oyster cultivation, the Stanway oyster cylinder, has been experimented in the basin of Arcachon since the autumn of 1989. The first observations on three species of juvenile bivalves showed that this system could be successfully used with the Japanese oyster Crassostrea gigas . A comparative study of the growth of this last species, reared in cylinders and oyster bags, has been carried out since spring 1990. The first results showed that the growth of juveniles and 18 months old oysters in cylinders is not as good as in bags. On the other hand, mortality did not differ between both techniques: less than 3%. This new system seems to be less efficient than the traditional French way of rearing oysters. However, an improvement in the quality...
Tipo: Text
Palavras-chave: Crassostrea gigas; Bivalvia; Juveniles; Oyster culture; Growth; Rearing.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-917.pdf

Nutritional value of six Pavlovophyceae for Crassostrea gigas and Pecten maximus larvae
Provedor de dados: 5
Autores: Ponis, Emanuele; Probert, I; Veron, Benoit; Le Coz, Jean-rene; Mathieu, Michel; Robert, Rene.
Four unidentified new strains of Pavlovophyceae [Pavlova sp. AC 250 (M), Pavlova sp. AC 248 (Psh), Pavlova sp. AC 251 (Pth), Pavlova sp. AC 538 (Psm)] and two known species [Pavlova pinguis (Ppi), Rebecca salina (ex Pavlova salina, Rsa)] were characterized (productivity, size, dry weight, ash, gross composition, fatty acids, sterols) and their nutritional value in bispecific diets were evaluated both on Crassostrea gigas and Pecten maximus larvae. All microalgae exhibited poor food value for C gigas larvae. P. pinguis and R. salina did not sustain any growth, like the control starved larvae. No exo-toxicity of P. pinguis and R. salina was detected. These two species were weakly ingested by C. gigas larvae, while the four other pavlovophytes were...
Tipo: Text
Palavras-chave: Biochemical composition; Larval nutrition; Nutritional value; Microalgae.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1558.pdf

Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique
Provedor de dados: 5
Autores: Pouvreau, Stephane; Rambeau, Marc; Cochard, Jean-claude; Robert, Rene.
Classically, investigation of the internal morphology and composition of molluscs and especially bivalves relies on destructive method (biometry, biochemistry and histology). These techniques have given essential information, but in contrast are time consuming and lead to the irreversible loss of the animal while they don't allow integration of the various levels of molecular-to-organism functioning. The aim of this study is to analyze for the first time the potential of NMR (nuclear magnetic resonance) imaging (MRI) to depict, with sufficient resolution and satisfactory contrast, the anatomy of a bivalve model, the Pacific oyster, Crassostrea gigas, without opening it. MRI experiments were carried out at 19 °C in several non-anaesthetized adult Pacific...
Tipo: Text
Palavras-chave: In vivo physiology; Aquaculture; Anatomy; MRI; Pacific oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1819.pdf

Etudes sur les mortalites larvaires de la coquille st Jacques Pecten maximus en ecloserie
Provedor de dados: 5
Autores: Robert, Rene; Miner, Philippe; Nicolas, Jean-louis; Mazuret, Michel; Connan, Jean-paul.
Larval mortalities occurring in molluscan hatcheries have often been associated with bacterial contamination. Although batches of oyster and clam larvae have been routinely reared in the hatchery of Argenton without antibiotics, high larval mortalities have been recorded with Pecten maxirnus under similar conditions. For this species, an addition of chloramphenicol was found necessary. However, this chemical has now been banned in Europe. Thus either substitition products or an improvement in the rearing procedures is essential. Studies carried out have shown that neither a decrease in larval density (1 larvae. ml"l) nor sea water change frequency (1 per day) had any positive effects. Furthermore, elective substances such as sugars were not suitable and...
Tipo: Text
Palavras-chave: Mortality; Pecten maximus; Hatchery; Larvae; Mollusc; Mortalité; Pecten maximus; Ecloserie; Larve; Mollusque.
Ano: 1995
URL: https://archimer.ifremer.fr/doc/1995/rapport-1568.pdf

Bivalve hatchery technology: The current situation for the Pacific oyster Crassostrea gigas and the scallop Pecten maximus in France
Provedor de dados: 5
Autores: Robert, Rene; Gerard, Andre.
The French oyster farming industry relies almost exclusively on juveniles collected from the natural environment; the supply of spat produced by hatcheries is low, about 10% of the industry's requirements. Development through selective breeding of oyster stocks which are better suited for aquaculture purposes, is likely to reverse this tendency since only hatcheries will be able to supply such animals. Scallop farming, which at present is poorly developed in France, relies exclusively on hatchery produced spat. Although hatchery technology is constantly being improved, significant production problems remain which must be solved before hatcheries become a major supplier of juveniles for the industry. This paper describes the present state of hatchery...
Tipo: Text
Palavras-chave: Pecten maximus; Crassostrea gigas; Hatchery; Molluscan rearing; Pecten maximus; Crassostrea gigas; Ecloserie; Élevage de bivalve.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-861.pdf

Developpement des veligeres de Crassostrea gigas dans le bassin d'Arcachon. Etudes sur les mortalites larvaires.
Provedor de dados: 5
Autores: His, Edouard; Robert, Rene.
Experiments in the hatchery rearing of C. gigas larvae showed that: the antifouling paints represent a real danger for spatting areas; the larvae of Arcachon breeding stocks grown in the seawater taken from the Bay develop normally in the laboratory; and, the growth of the larvae, collected from the Bay of Arcachon and reared in hatchery at 18 degree C, confirms that the last summer temperature regimes could not explain the abnormalities. It also confirms the good quality of the seawater of the bay for C. gigas reproduction.
Tipo: Text
Palavras-chave: Crassostrea gigas; Bivalvia; Environmental effects; Veligers; Oyster culture.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/publication-1835.pdf

Oyster sex determination is influenced by temperature - First clues in spat during first gonadic differentiation and gametogenesis
Provedor de dados: 5
Autores: Santerre, Christelle; Sourdaine, Pascal; Marc, Nicolas; Mingant, Christian; Robert, Rene; Martinez, Anne-sophie.
The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMI, Cg-SoxE, Cg-beta-catenin, Cg-Foxl2/Cg-FoxI2os) and of Oyvlg, a germ cell marker, were investigated by real-time PCR in spat grown at different temperatures (18, 22, 25 and 28 degrees C). In parallel, gonadic differentiation, gametogenesis and sex ratios were assessed by histology at each of these temperatures. Whatever the temperature, Cg-DMI, Cg-SoxE, Cg-beta-catenin and Oyvlg expressions peaked at the same developmental stage, always after Cg-Foxl2/Cg-Foxl2os (around 40-44 dpf for spat grown at 18 degrees C). Temperatures...
Tipo: Text
Palavras-chave: Oyster spat; Temperature; Sex ratio; TSD; Sex determination pathway.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00136/24767/22874.pdf

Nursery function of coastal temperate benthic habitats: New insight from the bivalve recruitment perspective
Provedor de dados: 5
Autores: Barbier, Pierrick; Meziane, Tarik; Foret, Martin; Tremblay, Rejean; Robert, Rene; Olivier, Frederic.
Marine habitat function has been typically investigated in terms of biogeochemical regulation but rarely in terms of population renewal, which is mainly controlled by recruitment dynamics. The recruitment phase is crucial for organisms with a bentho-pelagic life cycle, such as bivalves, and it regulates the population renewal success. This study provides new insight on the role of temperate benthic habitats on bivalve recruitment, as a function of nursery areas. Six dominant benthic habitats of the Chausey archipelago (Normandy, France) were studied. In each habitat, bivalve recruit assemblages were described at the end of two reproductive seasons. Furthermore, Ostrea edulis juveniles were immerged on each habitat during two months to compare growth...
Tipo: Text
Palavras-chave: Benthic habitats; Bivalve recruitment; Nursery function; Ostrea edulis; Fatty acids.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00367/47863/47845.pdf

Influence of feeding regime and temperature on development and settlement of oyster   Ostrea edulis   (Linnaeus, 1758) larvae
Provedor de dados: 5
Autores: Robert, Rene; Vignier, Julien; Petton, Bruno.
Under controlled conditions of food density and temperature, larval performances (ingestion, growth, survival and settlement success) of the flat oyster, Ostrea edulis, were investigated using a flow-through rearing system. In the first experiment, oyster larvae were reared at five different phytoplankton densities (70, 500, 1500, 2500 and 3500 μm3 μL−1: ≈1, 8, 25, 42 and 58 cells μL−1 equivalent TCg), and in the second, larvae were grown at four different temperatures (15, 20, 25 and 30°C). Overall, larvae survived a wide range of food density and temperature, with high survival recorded at the end of the experiments. Microalgae concentration and temperature both impacted significantly larval development and settlement success. A mixed diet of Chaetoceros...
Tipo: Text
Palavras-chave: Ostrea edulis; Larvae; Temperature; Feeding regime; Flow-through; DEB parameters.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00377/48817/49295.pdf

A New Photobioreactor for Continuous Microalgal Production in Hatcheries Based on External-Loop Airlift and Swirling Flow
Provedor de dados: 5
Autores: Loubiere, K; Olivo, Erell; Bougaran, Gael; Pruvost, J; Robert, Rene; Legrand, J.
This study deals with the scale of a new photobioreactor for continuous microalgal production in hatcheries. The combination of the state-of-art with the constraints inherent to hatcheries has turned the design into a closed, artificially illuminated and external-loop airlift configuration based on a succession of elementary modules, each one being composed of two transparent vertical interconnected columns. The liquid circulation is ensured pneumatically (air injections) with respect to a swirling motion (tangential inlets). A single module of the whole photobioreactor was built-up to scale its geometry (diameter and length) and to optimize its design (air sparger, tangential inlets). The volumetric productivities were predicted by modeling radiative...
Tipo: Text
Palavras-chave: Isochrysis affinis galbana; Swirling flow; Airlift; Hatchery; Continuous photobioreactor.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-5288.pdf

Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea
Provedor de dados: 5
Autores: Da Costa, Fiz; Le Grand, Fabienne; Quere, Claudie; Bougaran, Gael; Cadoret, Jean-paul; Robert, Rene; Soudant, Philippe.
Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates...
Tipo: Text
Palavras-chave: Tisochrysis lutea; Nitrogen limitation; Growth phase; Flow cytometry; Fatty acids; Lipid classes.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00399/51074/51936.pdf

Effects of copper chloride on the eggs and D larvae of Crassostrea gigas (Thunberg). Preliminary results
Provedor de dados: 5
Autores: His, Edouard; Robert, Rene.
Copper chloride is toxic to the embryonic development and D larvae of Crassostrea gigas and it affects t h e growth of the veligers at low concentrations.
Tipo: Text
Ano: 1981
URL: http://archimer.ifremer.fr/doc/00090/20166/17819.pdf

Nouvelle méthode pour étudier la nutrition de jeunes larves de Crassostrea gigas (Thunberg) en milieu naturel. Premières données expérimentales
Provedor de dados: 5
Autores: His, Edouard; Robert, Rene; Chretiennot-dinet, Marie-josèphe.
This paper describes a new technique for isolating unicellular algae which are thought to play a role ine the feeding fo bivalve larvae. The results are compared with the data obtained from experimental culture of bivalve larvae under controlled conditions. Effectively ingested algal strains can be isolated by this technique. The food value of one strain obtained as monsopecific culture is demonstrated.
Tipo: Text
Ano: 1985
URL: http://archimer.ifremer.fr/doc/00021/13255/10301.pdf

Action de l'érythromycine et de l'acide oxolinique sur le déveloloppement larvaire de la coquille St Jacques Pecten maximus
Provedor de dados: 5
Autores: Robert, Rene; Nicolas, Jean-louis; Conan, Rony.
For lack of antibiotics, King scallop larviculture faces high mortalities. In the experimental mollusc hatchery of Argenton (Brittany, France), the presence of a vibrio, Vibrio pectinicida, associated with the stocks seems to be responsible for this low survival rate. As prophylactic methods are difficult to implement in the current structures (water sterilization, egg decontamination, isolation of the stocks), the addition of chloramphenicol is the only solution to protect the stocks. However, the use of this antibiotic, considered as dangerous for man, has been prohibited in animal production throughout the whole of Europe since 1994. The action of two other antibiotics, the erythromycine and the oxolinic acid, has been searched for in Pecten maximus...
Tipo: Text
Palavras-chave: Acide oxolinique; Erythromycine; Antibiotiques; Coquille St Jacques; Pecten maximus L.; Développement larvaire.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-6199.PDF

Evolution spatio-temporelle des paramètres hydrobiologiques dans le bassin d'Arcachon (juillet 1984-juillet 1985)
Provedor de dados: 5
Autores: Robert, Rene; Guillocheau, N.
L'évolution des paramètres hydrobiologiques du bassin d'Arcachon a été étudiée de juillet 1984 à juillet 1985. Ces observations confèrent à cette période un caractère exceptionnel pour la baie.
Tipo: Text
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00081/19232/16827.pdf

La production aquacole de coquille Saint-Jacques (Pecten maximus) et le soutien scientifique à l'operation pilote
Provedor de dados: 5
Autores: Dao, Jean-claude; Barret, Jean; Carval, Jean-pierre; Connan, Jean-paul; Corre, Christian; Devauchelle, Nicole; Fleury, Pierre-gildas; Laurent, Pascale; Mazuret, Michel; Miner, Philippe; Mingant, Christian; Muzellec, Marie-louise; Robert, Rene.
Aquaculture production of scallop has been identified by a series of technical operations during the past ten years programme under a partnership involving the fishermen organizations with regional and national grants. 3 million juveniles have been produced annually, giving a potential recapture by fishing of 100 to 120 tonnes 3 years later. Results in terms of technical and economical approach have pointed out the principal unknown factors responsable for high costs. 7he pilot programme aims to increase the production to reach a level of 5 million juveniles to support the fishery of the rade de Brest and induce new management of the resources. Two priorities were selected, control of the reproduction in the hatchery to obtain reliable larval and...
Tipo: Text
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00044/15494/12860.pdf

Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas
Provedor de dados: 5
Autores: Da Costa Gonzalez, Fiz; Robert, Rene; Quere, Claudie; Wikfors, Gary H.; Soudant, Philippe.
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5...
Tipo: Text
Palavras-chave: Fatty acid; Larvae; Lipids; Metabolism; Oyster; Synthesis.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00268/37962/36086.pdf

Observations on larval development and settlement of Patinofecten yessoensis in hatcheries
Provedor de dados: 5
Autores: Robert, Rene; Miner, Philippe; Mazuret, Michel; Connan, Jean-paul.
The influence of density on Patinopecten yessoensis larval development was studied during production of an F2 generation. Setting behaviour of P. yessoensis on PVC removable spat collectors, previously tested with Pecten maximus, was determined. Poor larval growth of P. yessoensis was observed at a larval density of 7 ·ml·1 and post larval settlement occurred mainly on the bottom of the baskets, as has been observed with the king scallop, P. maximus. The PVC collector is not suitable for either species of scallop.
Tipo: Text
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00090/20163/17816.pdf

Individual monitoring of gonad development in the European flat oyster Ostrea edulis by in vivo magnetic resonance imaging
Provedor de dados: 5
Autores: Davenel, Armel; Gonzalez, Ricardo; Suquet, Marc; Quellec, Stephane; Robert, Rene.
Previous studies have shown that magnetic resonance imaging is a very appropriate non-invasive technique for quantifying the growth of somatic and gonadic tissues and sex determination in the Pacific oyster, Crassostrea gigas. Despite a thinner gonad, harder to distinguish than that of the Pacific oyster, we showed in this study that it was possible to distinguish and quantify the development of the gonad of the European flat oyster, Ostrea edulis, using a 3D MRI sequence with a suitable finer resolution. Compared to T2-weighted images, theoretically the most appropriate for good anatomical description, T1-weighted images were more suited to gonad observation. The gonad development was quantified by the number of voxels with higher grey level. Larvae were...
Tipo: Text
Palavras-chave: Oyster; Ostrea edulis; Gonad development; Magnetic resonance imaging.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12508/9363.pdf

Experimental shift of diet and DIC stable carbon isotopes: Influence on shell delta C-13 values in the Manila clam Ruditapes philippinarum
Provedor de dados: 5
Autores: Poulain, C.; Lorrain, Anne; Mas, R.; Gillikin, D. P.; Dehairs, F.; Robert, Rene; Paulet, Yves-marie.
The influences of diet and seawater dissolved inorganic carbon (DIC) on the carbon isotope composition of shell aragonite (Cinched in the Manila clam Ruditapes philippinarum reared under laboratory conditions were investigated. Clams were exposed to two successive negative carbon isotope shifts: a first shift in diet (delta C-13(phytoplankton)) and a second shift, 35 days later, in DIC (delta C-13(DIC)). Both successive shifts induced a decrease in delta C-13(shell). These results are the first to experimentally confirm an incorporation of respired carbon derived from food and carbon from DIC into shell carbonate of adult bivalves. Skeletal delta C-13 responded to changes in the delta C-13 of both diet and DIC in less than 7 days. Consequently, proxies...
Tipo: Text
Palavras-chave: Delta C-13(shell); DIC; Salinity; Metabolic carbon; Ruditapes philippinarum; Estuarine water; Proxy.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11443/8001.pdf

Scallop larval survival from erythromycin treated broodstock after conditioning without sediment
Provedor de dados: 5
Autores: Holbach, Marine; Robert, Rene; Boudry, Pierre; Petton, Bruno; Archambault, Philippe; Tremblay, Rejean.
Pathogenic bacteria are known to be one of the main factors affecting Pecten maximus larval survival in hatcheries. As a result, juvenile production often relies on the use of antibiotics during larval culture. However, limitations of the usage of chemicals such as chloramphenicol in aquaculture have been reinforced due to their negative environmental impact and alternatives are accordingly needed. Thus, the importance of bacterial transfer from oocytes to larvae has been studied here as well as procedures to limit larval mortality in P. maximus. In order to reduce bacterial contamination during larval development, we focused on two periods, broodstock conditioning and post-fertilization. The animals were conditioned for 2 months with two erythromycin...
Tipo: Text
Palavras-chave: Scallop; Broodstock conditioning; Larval survival; Vibriosis.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00240/35171/33675.pdf

The effects of eight single microalgal diets on sex-ratio and gonad development throughout European flat oyster (Ostrea edulis L.) conditioning
Provedor de dados: 5
Autores: Gonzales, Ricardo; Quillien, Virgile; Robert, Rene.
To determine the effects of food quality on Ostrea edulis reproduction, European flat oysters were conditioned during two sets of experiments, carried out in spring and autumn, during 40 days at 19 degrees C, in 50 l transparent flow-through tanks, in triplicate, and fed constantly at 900 mu m(3) mu l(-1), with eight different types of microalgae. Four species were fed per group of trials: Isochrysis affinis galbana, Chaetoceros gracilis, Skeletonema marinoi, and Tetraselmis suecica were fed to flat oysters in the first set; whereas Rhodomonas salina, Thalassiosira weissflogii, Thalassiosira pseudonana, and Pavlova lutheri were provided during the second set of experiments. At the beginning and end of both conditioning periods, oysters were sampled and...
Tipo: Text
Palavras-chave: Ostrea edulis; Broodstock conditioning; Gametogenesis; Microalgal diets.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00144/25497/24163.pdf

Influence of phytoplankton diet mixtures on microalgae consumption, larval development and settlement of the Pacific oyster Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Rico Villa, Benjamin; Le Coz, Jean-rene; Mingant, Christian; Robert, Rene.
Microalgae commonly used as feed for bivalves, Pavlova lutheri (P), Isochrisis affinis galbana (T) and Chaetoceros calcitrans forma pumilum (Cp), were fed to Pacific oyster Crassostrea gigas to assess their nutritional value for larval development and metamorphosis during two experiments. Monospecific, bispecific and trispecific diets were firstly evaluated during 3 weeks from D larvae to young postlarvae. Then bispecific diets, based on different T and Cp proportions, were assessed during a similar period. Concurrently, ingestion was studied through the whole larval and postlarval development for each diet and/or diet mixture. Because lipids are assumed to be a key nutrient for bivalves, biochemical analysis was undertaken on the second set of trials...
Tipo: Text
Palavras-chave: Lipids; Grazing; Metamorphosis; Growth; Larvae; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1707.pdf

Dispositifs de concentration de poissons en Polynésie française (La Pêche Maritime)
Provedor de dados: 5
Autores: Ugolini, B.; Robert, Rene.
Tipo: Text
Ano: 1982
URL: http://archimer.ifremer.fr/doc/00090/20167/17820.pdf

An evaluation of Mg/Ca, Sr/Ca, and Ba/Ca ratios as environmental proxies in aragonite bivalve shells
Provedor de dados: 5
Autores: Poulain, C.; Gillikin, D. P.; Thebault, Julien; Munaron, Jean-marie; Bohn, M; Robert, Rene; Paulet, Yves-marie; Lorrain, Anne.
The influence of salinity and water chemistry on Mg/Ca, Sr/Ca and Ba/Ca ratios in the aragonitic shells of the Manila clam were investigated. Clams were reared at constant temperature (20 °C) under different controlled conditions of salinity, commonly encountered in their natural habitat. Clams were held in three tanks with a constant salinity of 35 for the first 35 days and then two tanks were changed to lower salinities (20 and 28) for the next 29 days. Individual shell Mg/Ca, Sr/Ca and Ba/Ca ratios were studied through time. Despite stable conditions (temperature, salinity, and Mg/Cawater) for clams reared at salinity 35 during the experiment, Mg/Ca shell ratios increased through the time. Moreover the salinity decrease at t35 slowed the increase of...
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Aragonite; Sclerochronology; Bivalved mollusk; Trace element; Proxy.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00245/35649/34158.pdf

Nutrional inadequacy of Nannochloris atomus and Stichoccocucs bacillaris for the oyster Crassostre gigas (Thunberg) larvae
Provedor de dados: 5
Autores: Robert, Rene.
The evaluation of Nannochloris atomliS and StichoccocllS bacillaris as nutrient sources for oyster larvae has been attempted in the present work. After a study of their grawth and size distributions, these two species were used as food for Crassostrea gigas larvae. Despite praper sizes (2.5 to 20 µm3) the larval development of Crassostrea gigas was poor. Evaluation of ingestion and digestion of both algae, by means of epifluorescence microscopy, on 2 day and 6 day old larvae showed that they were normally ingested but weakly digested. Consequently, their nutritional inadequacy for larval molluscs may be also related to their indigestibility.
Tipo: Text
Palavras-chave: Stichococcus bacillaris; Nannochloris atomus; Larvae; Nutrient sources; Huître; Crassostrea gigas; Nutrition; Stichococcus bacillaris; Nannochloris atomus; Larve; Algue fourrage; Huître; Crassostrea gigas; Nutrition.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-6198.PDF

Pavlova lutheri: Production, preservation and use as food for Crassostrea gigas larvae
Provedor de dados: 5
Autores: Ponis, Emanuele; Parisi, G; Chini Zittelli, G; Lavista, F; Robert, Rene; Tredici, M. R.
The haptophyte Pavlova lutheri was cultivated in a 4-l flat alveolar photobioreactor (FAP) in semi-continuous mode during 35 days, achieving a mean volumetric productivity of 0.4 g l-1 day-1. The microalgal culture was harvested from the photobioreactor at an average concentration of 3.5 g l-1 and subjected to different storage conditions in which the effects of temperature (1 °C, 4 °C and 25 °C), air supply and preservative (ascorbic or propionic acid) addition on the viability of the stored suspensions were investigated. Low temperatures (1 and 4 °C) and air-bubbling extended the shelf-life of the stored suspension, while no beneficial effects of ascorbic or propionic acid were found. The influence of temperature and air-bubbling on fatty acid content...
Tipo: Text
Palavras-chave: Crassostrea gigas; Fatty acid composition; Mollusc nutrition; Photobioreactor; Pavlova lutheri; Preservation; Algal suspensions.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4194.pdf

Evaluation of toxicity of Deepwater Horizon slick oil on spat of the oyster Crassostrea virginica
Provedor de dados: 5
Autores: Vignier, Julien; Rolton, Anne; Soudant, Philippe; Chu, Fu-lin E.; Robert, Rene; Volety, Aswani K.
The 2010 explosion of the Deepwater Horizon (DWH) oil rig generated the largest marine oil spill in US history with millions of barrels of crude oil released in the Gulf of Mexico (GoM). The eastern oyster, Crassostrea virginica, is an ecologically and economically important species in the northern GoM. Due to its biological characteristics (sessile, filter feeding), juvenile oysters may have been affected. This study investigated the effects of surface-collected DWH oil prepared as high-energy water-accommodated fraction (HEWAF) on the survival of 2-month-old oyster spat, and evaluated the potential impacts of HEWAF on particle clearance rate and spat tissue. Exposure of oysters to a range of oil/HEWAF (0-7-66-147-908-3450 mu g tPAH50 (sum of 50...
Tipo: Text
Palavras-chave: Oyster spat; Deepwater Horizon oil; Sublethal; Tissue; Inflammatory response; Histopathology.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00425/53615/54548.pdf

Growth, fatness and gross biochemical  composition of the japanese oyster Crassostrea gigas in stanway cylinders in the bay of Arcachon, France
Provedor de dados: 5
Autores: Robert, Rene; Trut, Gilles; Borel, Michel; Maurer, Daniele.
The Stanway cylinder was tested in the bay of Arcachon from September 1989 to August 1991. Trials were mainly performed on the Japanese oyster Crassostrea gigas in a wind-exposed area. The seed, spat, 18 month old oysters development and fattening of 2 year old oysters were studied. Compared to plastic bags the Stanway cylinder has high positive effects on survival and growth of Crassostrea gigas seed. It also has positive effects on commercial sized Crassostrea gigas fattening. Nevertheless, the cylinder has negative effects on the growth of Crassostrea gigas spat and 18 month old oysters, except for width. However, oyster quality is greatly improve : higher condition index, higher carbohydrate contents and higher shell densities were recorded. Because...
Tipo: Text
Palavras-chave: Bay of Arcachon; Culture methods; Growth; Crassostrea gigas; Bassin d'Arcachon; Technique d'élevage; Croissance; Crassostrea gigas.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/rapport-1644.pdf

The food value of starch rich flagellates for Pecten maximus (Linné) larvae. Preliminary results
Provedor de dados: 5
Autores: Robert, Rene; Moal, Jeanne; Campillo, Maria-josé; Daniel, Jean-yves.
Pecten maximus larvae exhibit amylase as a main digestive enzyme indicating the possibility of starch hydrolysis. The food value of Rhodomonas salina and Porphyridium cruentum, both rich in starch, on the development of Pecten maximus larvae was investigated. The larval development was studied by means of an image analysis technique and epifluorescence microscopy was used for detecting ingestion and digestion of each phytoplankton species. The biochemical contents of algae and larvae were also assessed. The best growth of Pecten maximus larvae was observed when fed with the mixture PTS (plurispecific control). No growth was observed for larvae fed with Porphyridium cruentum of which most of the population died on day 16 (90%). This algal species was...
Tipo: Text
Palavras-chave: Larvae; Food value; Pecten maximus.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-6192.PDF

Effects of hydrodynamic factors on     Pecten maximus     larval development
Provedor de dados: 5
Autores: Holbach, Marine; Robert, Rene; Miner, Philippe; Mingant, Christian; Boudry, Pierre; Tremblay, Rejean.
Hatchery production of great scallop, Pecten maximus, remains unpredictable, notably due to poor larval survival. Large-scale flow-through systems up to 3500 L have been developed to avoid the use of antibiotics in static systems. Alternatively, small-scale flow-through systems have been successfully applied for oysters but they proved to be unsuitable to rear scallop larvae. By focusing on physical factors presumed to limit P. maximus larval development, this study aimed to optimize great scallop larvae rearing parameters under controlled conditions. First, the influence of aeration on larval performances, energetic metabolism and antioxidant defences were studied both in static and flow-through systems. Aeration depressed larval food intake, regardless...
Tipo: Text
Palavras-chave: Aeration; Flow-through; Larval physiology; Pecten maximus.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00384/49508/51151.pdf

Croissance des larves de Crassostrea gigas et de Mytilus galloprovincialis en présence d'algues monocellulaires isolées du tractus digestif des véligères du milieu naturel
Provedor de dados: 5
Autores: His, Edouard; Robert, Rene.
The collection of Crassostrea gigas veligeres in their natural environment and the homogenate technique (HIS et al., 1985) enable the monospecific culture of nine algae which larvae feed on. Four of them could be mass-produced: Nannochloris atomus, Stichococcus bacillaris, Chlamidomonas bullosa and Tetraselmis Tetrathele. Their feeding quality has been tested using the cross-growth method, and using Crassostrea gigas and Mytilus galloprovincialis veligeres. Although regularly found in the homogenates, and thus fed on by larvae, the first three algae gave poor results. Only Tetraselmis tetrathele has good nutritional qualities for Crassostrea gigas veligeres.
Tipo: Text
Palavras-chave: Tetraselmis tetrathele; Chlamydomonas bullosa; Stichococcus bacillaris; Nannochloris atomus; Algues unicellulaires; Mytilus galloprovincialis; Crassostrea gigas; Nutrition; Larves.
Ano: 1986
URL: http://archimer.ifremer.fr/doc/1986/acte-6186.PDF

Action d'un sel organo-métallique, l'acétate de tributyle-étain sur les oeufs et les larves D de Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: His, Edouard; Robert, Rene.
L'action de l'acétate de tributyle-étain, à des concentrations variant de 5 à 100 micro- grammes/l a été étudiée sur les oeufs de Crassostrea gigas en cours de fécondation. Parallèlement les mêmes concentrations ont été testées sur les larves D âgées de 24 heures.
Tipo: Text
Palavras-chave: Huître; Crassostrea gigas; Expérimentation; Larves; Reproduction; Sel organo-métallique; Acétate de tributyle étain.
Ano: 1980
URL: http://archimer.ifremer.fr/doc/00009/11983/8687.pdf

Interet du compteur de particules ZB-ZBI et de l'analyseur C1000 pour la numeration des algues unicellulaires de culture.
Provedor de dados: 5
Autores: Robert, Rene.
Marine unicellular algae growth rates were studied with: a Coulter Counter in conjunction with a channelizer, and a hemacytometer. Compared to the hemacytometer a good precision of the culture density was observed when the Coulter Counter was used in conjunction with a channelizer. Nevertheless care had to be taken when determining the growth of the diatoms.
Tipo: Text
Palavras-chave: Laboratory equipment; Growth; Particle counters; Laboratory culture; Algal culture.
Ano: 1985
URL: http://archimer.ifremer.fr/doc/1985/publication-1777.pdf

Ontogenic changes in the contents of dopamine, norepinephrine and serotonin in larvae and postlarvae of the bivalve Pecten maximus
Provedor de dados: 5
Autores: Cann Moisan, Christine; Nicolas, Laurence; Robert, Rene.
In the present study, norepinephrine (NE), epinephrine (E), dopamine (DA) and 5-hydroxy-tryptamine (5HT) have been quantified by high performance liquid chromatography (HPLC) in Pecten maximus during larval and post-larval development. With average values ranging from 2 to 5 pg mug(-1) of protein, NE remained low through the whole larval life, while epinephrine (E) was undetected. DA and 5HT were the most abundant monoamines with significant variations between larval, pre-metamorphic and post-larval stages. During the first 20 days, corresponding to D larval and umboned larval stages, levels of DA and 5HT increased from 15 to 30 and 10 to 15 pg mug(-1) of protein, respectively. Then during the last week of larval life, at the approach of metamorphosis, DA...
Tipo: Text
Palavras-chave: Pecten maximus; Metamorphosis; Post larvae; Larvae; Monoamines.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-568.pdf

Microalgues et nutrition  larvaire en écloserie de mollusques
Provedor de dados: 5
Autores: Robert, Rene; Trintignac, Pascal.
The production of live microalgae remains the only reliable technique for feeding bivalve mollusc larvae in a controlled environment. However, these algae must answer a number of size, nutritive value and mass-production criteria. The current knowledge in this field is assessed in this work which will lead us to the conclusion that only a few species meet these requirements.
Tipo: Text
Palavras-chave: Algues fourrage; Mollusque; Ecloserie; Microalgue; Nutrition; Elevage larvaire.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/publication-6197.PDF

Determination of stocking density limits for Crassostrea gigas larvae reared in flow-through and recirculating aquaculture systems and interaction between larval density and biofilm formation
Provedor de dados: 5
Autores: Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis.
The first aim of this study was to determine the stocking density limits for Pacific oyster Crassostrea gigas larvae reared in flow-through system (FTS) and recirculating aquaculture systems (RAS). The second aim was to examine biofilm formation on the larval tank wall and its interaction with larvae growth. Three larvae concentrations were tested: 50, 150, and 300 mL−1. Chemical parameters and larvae performance were measured. The biofilm was observed by scanning electron microscopy, and its bacterial composition was investigated by pyrosequencing analysis of part of the 16S rRNA gene. The highest growth (13 µm day–1), survival (87%) and metamorphosis (50%) rates were observed in FTS at 50 larvae mL–1, while lower and similar performances occurred at...
Tipo: Text
Palavras-chave: Pacific oyster; Larval culture; High stocking density; Biofilm; Filamentous bacteria.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00395/50611/51324.pdf

Impacts of Deepwater Horizon oil and associated dispersant on early development of the Eastern oyster Crassostrea virginica
Provedor de dados: 5
Autores: Vignier, J.; Donaghy, L.; Soudant, P.; Chu, F. L. E.; Morris, J. M.; Carney, M. W.; Lay, C.; Krasnec, M.; Robert, Rene; Volety, A. K.
The explosion of the Deepwater Horizon (DWH) oil platform resulted in large amounts of crude oil and dispersant Corexit 9500A® released into the Gulf of Mexico and coincided with the spawning season of the oyster, Crassostrea virginica. The effects of exposing gametes and embryos of C. virginica to dispersant alone (Corexit), mechanically (HEWAF) and chemically dispersed (CEWAF) DWH oil were evaluated. Fertilization success and the morphological development, growth, and survival of larvae were assessed. Gamete exposure reduced fertilization (HEWAF: EC201 h = 1650 μg tPAH50 L− 1; CEWAF: EC201 h = 19.4 μg tPAH50 L− 1; Corexit: EC201 h = 6.9 mg L− 1). CEWAF and Corexit showed a similar toxicity on early life stages at equivalent nominal concentrations....
Tipo: Text
Palavras-chave: Deepwater Horizon oil; Oyster; Fertilization; CEWAF; Corexit; PAH.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00277/38853/42693.pdf

Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas
Provedor de dados: 5
Autores: Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre.
Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combination with OsHV-1 DNA quantification by standard real-time PCR and total vibrio population levels in oyster tissues. To examine the effect of seawater temperature on disease transmission and related mortality of oysters, cohabitation experiments were conducted between healthy naïve oysters and oysters previously exposed to field conditions in areas where mortalities were occurring. Oysters initially maintained in controlled conditions (free of mortality and negative...
Tipo: Text
Palavras-chave: Juvenile oyster; Pathology; Temperature; Survival; Vibrios; Ostreid herpesvirus 1.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00148/25891/23912.pdf

Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds
Provedor de dados: 5
Autores: Knuckey, R; Brown, M; Robert, Rene; Frampton, D.
A novel technique was developed for the flocculation of marine microalgae commonly used in aquaculture. The process entailed an adjustment of pH of culture to between 10 and 10.6 using NaOH, followed by addition of a non-ionic polymer Magnafloc LT-25 to a final concentration of 0.5 mg L&amp;#8722;1. The ensuing flocculate was harvested, and neutralised giving a final concentration factor of between 200- and 800-fold. This process was successfully applied to harvest cells of Chaetoceros calcitrans, C. muelleri, Thalassiosira pseudonana, Attheya septentrionalis, Nitzschia closterium, Skeletonema sp., Tetraselmis suecica and Rhodomonas salina, with efficiencies &amp;#8805;80%. The process was rapid, simple and inexpensive, and relatively cost neutral with...
Tipo: Text
Palavras-chave: Chaetoceros; Thalassiosira; Flocculation; Concentrate; Bivalve; Algal paste.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1820.pdf

New microalgae for the Pacific oyster Crassostrea gigas larvae
Provedor de dados: 5
Autores: Ponis, Emanuele; Probert, Ian; Veron, Benoit; Mathieu, Michel; Robert, Rene.
The number of microalgae of high nutritional value actually available to mollusc hatcheries is limited to a few species and this represents a constraint for the industry. Fifteen microalgal species belonging to seven different taxonomic classes were selected from the Algobank-Caen microalgal culture collection of the University of Caen. An initial screening of these microalgae, based on their cytomorphological characteristics and growth performances, led to the selection of four species (Imantonia rotunda, Emiliania huxleyi, Pseudoisochrysis paradoxa and Diacronema vlkianum), which were then tested as food for Crassostrea gigas larvae. Two species (L rotunda and E huxleyi) were of poor food value for oyster larvae, while P paradoxa and D. vlkianum resulted...
Tipo: Text
Palavras-chave: Biochemical composition; Larval nutrition; Mollusc hatchery; Microalgae.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1547.pdf

Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas
Provedor de dados: 5
Autores: Fabioux, Caroline; Huvet, Arnaud; Lelong, C; Robert, Rene; Pouvreau, Stephane; Daniel, Jean-yves; Mingant, Christian; Le Pennec, M.
The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-mount in situ hybridization and real-time PCR. The Oyvlg transcripts appeared to be localized to the vegetal pole of unfertilized oocytes and maternally transmitted to embryos. At early development, these maternal transcripts were observed to segregate into a single blastomere, from the CD macromere of 2-cell stage to the 4d mesentoblast of blastula. From late blastula stage, the mesentoblast divided into two cell clumps that migrated to both sides of the larvae...
Tipo: Text
Palavras-chave: Primordial germ cells; Oyster vasa like gene; Germline; Development; Crassostrea gigas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-648.pdf

Impact of temperature on larval development and evolution of physiological indices in Crassostrea gigas
Provedor de dados: 5
Autores: Ben Kheder, Rym; Moal, Jeanne; Robert, Rene.
The effect of four temperatures, ranging between 17 degrees C and 32 degrees C, was studied on development and lipid reserve management of Crassostrea gigas larvae. No effect of temperature was found on larval mortality, as high survival (&gt;90%) was recorded before competence at all temperatures studied. Temperature did, nonetheless, have a strong effect on growth and settlement success. At low temperature (17 degrees C), larvae competent to metamorphose were only observed from day 23 and only a low percentage finally achieved metamorphosis (12%). The opposite was seen at temperatures &gt;= 27 degrees C: larval competence appeared at day 18 and led to high rates of metamorphosis (60-90%). This difference at settlement seemed to be linked to larval...
Tipo: Text
Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Temperature; Lipids; Physiological indices.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00019/13046/10146.pdf

Analysis of larval oyster grazing by flow cytometry
Provedor de dados: 5
Autores: Chretiennot-dinet, Marie-josèphe; Vaulot, Daniel; Galois, Robert; Spano, Anna-maria; Robert, Rene.
The ingestion of 8 algal species by oyster larvae (Crassostrea gigas Thunberg, 1793) was measured by flow cytometry (FCM). In a preliminary experiment, cell size (estimated by light scatter) and chlorophyll fluorescence of 30 algal species were evaluated to select those species which could be mixed together and still be easily discriminated by FCM. Grazing experiments were carried out over 48 h with 6 and 15-day old larvae fed on 3 algal mixtures, each containing 3 different algal species. The concentration of each species was estimated at 0, 6, 12, 24 and 48 h by FCM. Grazing pressure on a given algal species was dependent upon the age of the larvae, the time of the day and the composition of the mixture. Grazing rates of older larvae were about twice...
Tipo: Text
Palavras-chave: Oyster larvae; Crassostrea gigas; Grazing; Algae; Selective feeding; Flow cytometry.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00090/20164/17817.pdf

In-situ microcosms, a tool for assessment of pesticide impacts on oyster spat (Crassostrea gigas)
Provedor de dados: 5
Autores: Stachowski Haberkorn, S; Quiniou, Francoise; Nedelec, M; Robert, Rene; Limon, G; De La Broise, D.
Effects of the herbicide Basamais (bentazon) and the fungicide Opus (epoxiconazole) on oyster spat (Crassostrea gigas) were assessed using in-situ microcosms in a field experiment lasting 13 days. Six-week-old hatchery spat (mean size 1.1 mm), previously collected on PVC plates, was immersed in glass bottles filled with 200 mu m filtered seawater. Bottles were maintained underwater at 6 m depth and their water content changed every other day. Growth, measured as shell area index increase, was 126 +/- 4% in the control bottles. While no growth differences were observed between control and individual pesticide treatments at 10 mu g l(-1), oysters treated with a mix of 10 mu g l(-1) Opus and 10 mu g l(-1) Basamais showed a 50% growth reduction compared with...
Tipo: Text
Palavras-chave: Epoxiconazole; Bentazon; Pesticide; Toxicity; Oyster; Microcosm.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4134.pdf

Influence of temperature on disease transmission associated with ostreid herpes virus OSHV-1 µVAR in relation to survival of juvenile Crassostrea gigas
Provedor de dados: 5
Autores: Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre; Alunno-bruscia, Marianne.
Since 2008, mass mortalities of 1-yr-old oysters Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have occurred along all coasts of France. These mortality events represent the most serious crisis for the French oyster industry since the introduction of C. gigas in the early 1970s. Temperature is commonly one of the major triggering factors of disease epizootics, notably for aquatic species. In France, OsHV-1 is generally detected in dying oysters when seawater temperature is higher than 16° C. The influence of temperature on OsHV-1 detection and expression was also demonstrated for C. gigas larvae and suspected for juveniles. The present study aims to characterize the effect of temperature on disease transmission and survival related...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00151/26259/24343.pdf

Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries
Provedor de dados: 5
Autores: Marchetti, Julie; Bougaran, Gael; Le Dean, Loic; Megrier, Cyril; Lukomska, Ewa; Kaas, Raymond; Olivo, Erell; Baron, Regis; Robert, Rene; Cadoret, Jean-paul.
Optimal culture conditions were investigated for the continuous culture of Isochrysis affinis galbana (T-Iso) by assessing the effects of irradiance, temperature, pH and nitrogen and phosphorus requirements. Assessment of the growth rate-irradiance relationship in a turbidostat experiment highlighted that an optimum irradiance efficiency for growth (E-mu) occurred at irradiance much lower than the saturating level. Furthermore, our modeling approach for productivity pointed out that an optimal set of irradiance and dilution rate could be achieved in order to maximize irradiance efficiency. Optimal conditions for growth were assessed for temperature, pH and irradiance by means of a factorial design experiment. The resulting model predicted that optimal...
Tipo: Text
Palavras-chave: Isochrysis affinis galbana; Irradiance efficiency; Factor interaction; Nutrient limitation; Microalgae cultures.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00076/18736/16465.pdf

The selection of an ideal diet for Ostrea edulis (L.) broodstock conditioning (part B)
Provedor de dados: 5
Autores: Gonzalez-araya, Ricardo; Lebrun, Luc; Quere, Claudie; Robert, Rene.
Four microalgae species (Rhodomonas salina, Thalassiosira weissflogii, Thalassiosira pseudonana and Pavlova lutheri) were evaluated to estimate their potential as food for Ostrea edulis (L.) reproductive conditioning. Best ingestion and absorption were observed with R. salina (3.44 and 1.59 mg g− 1 h− 1, respectively), followed by T. pseudonana (2.75 and 0.98 mg g− 1 h− 1) and P. lutheri (2.40 and 0.91 mg g− 1 h− 1). Oysters fed T. weissflogii exhibited the lowest ingestion and absorption values (1.40 and 0.68 mg g− 1 h− 1). Proximate composition (proteins and carbohydrates) and lipid content (fatty acids and sterols) analysed in four main tissues (gonad, digestive gland, muscle and gills) also differed significantly with diet. Protein ranged from 355 mg...
Tipo: Text
Palavras-chave: Ostrea edulis; Conditioning; Algal diets; Ingestion; Absorption; Biochemical composition.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00090/20092/17731.pdf

Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition
Provedor de dados: 5
Autores: Da Costa, F.; Petton, Bruno; Mingant, Christian; Bougaran, Gael; Rouxel, Catherine; Quere, Claudie; Wikfors, G. H.; Soudant, P.; Robert, Rene.
Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate...
Tipo: Text
Palavras-chave: Larvae; Lipids; Oyster; Tisochrysis lutea.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00251/36258/36063.pdf

Comparaison de deux méthodes de prélèvement pour le dénombrement des larves de Crassostrea gigas en zone conchylicole
Provedor de dados: 5
Autores: His, Edouard; Borel, Michel; Robert, Rene; Laborde, Jean-louis.
La numération des larves d'huître dans le plancton renseigne les ostréiculteurs sur la date de pose des collecteurs. La technique utilisée est très ancienne (BOURY, 1928) ; pour chaque station trois prélèvements par traits de filets pendant 10 minutes sont effectués : deux en surface (filet 200 et filet 130) et un à 1 mètre de profondeur (filet 130). Une méthode par pompage avec mesure instantanée du volume d'eau filtrée a été mise au point (prélèvements en surface et à 1 mètre de profondeur, et rétention des véligères sur filets 200). La comparaison des deux techniques met en évidence l'inadaptation de l'ancienne méthode. Le choix de la porosité (70 µm pour les filets 200) est mal adapté à la récolte des larves D (stade 1) dont le nombre peut être sous...
Tipo: Text
Palavras-chave: Numération; Technique; Reproduction; Crassostrea gigas; Larves; Naissain; Recrutement; Ostréiculture.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/1987/acte-6444.PDF

Competition for food in the larvae of two marine molluscs, Crepidula fornicata and Crassostrea gigas
Provedor de dados: 5
Autores: Blanchard, Michel; Pechenik, J; Giudicelli, E; Connan, Jean-paul; Robert, Rene.
The degree to which larvae of the invasive American slipper limpet (Crepidula fornicata) and the Japanese oyster (Crassostrea gigas) may compete for food was examined during 2003 in the laboratory. Larval microalgae uptake, growth and mortality were compared for larvae fed each of six species of unicellular algae, ranging in length from 2 to 10 mu m. Tested diets included the two flagellates Tetraselmis chui (Prasinophyceae) and Isochrysis affinis galbana (T-ISO, Haptophyceae), one member of the Chlorophyceae (Nannochloris atomus), and three diatom species (Chaetoceros calcitrans forma pumilum, Chaetoceros gracilis, Skeletonema marino). We found that the limpet larvae ingested phytoplankton over a wider range of cell sizes and ate at higher rates on each...
Tipo: Text
Palavras-chave: Larvae; Crepidula fornicata; Crassostrea gigas Growth Feeding rate Diet Phytoplankton.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4703.pdf

Croissance et mortalite du petoncle noir Chlamys varia dans le bassin d'Arcachon, France
Provedor de dados: 5
Autores: Trut, Gilles; Robert, Rene; Laborde, Jean-louis.
Until 1960, the Black scallop Chlamys varia was relatively abundant along the French Atlantic coasts. Today most of these natural beds have disappeared. Because mollusc hatchery and nursery techniques have been mastered, the reintroduction of this species, under control, was carried out in several areas and particularly in the bay of Arcachon, from 1989 to 1992. Good results were obtained in hatchery and nursery but the development of the juveniles in the natural fields was poor. The growth of Chlamys varia was satisfactory but heavy mortalities were rapidly recorded which could not be related to the hydrobiological conditions. This species seems to be very sensitive to short emersion and removal operations which are incompatible with aquaculture processes.
Tipo: Text
Palavras-chave: Bassin d'Arcachon; Mortalité; Croissance; Chlamys varia; Mollusque; Bay of Arcachon; Mortality; Growth; Chlamys varia; Mollusc.
Ano: 1994
URL: https://archimer.ifremer.fr/doc/1994/rapport-1567.pdf

Bacterial problems associated with scallop Pecten maximus larval culture
Provedor de dados: 5
Autores: Nicolas, Jean-louis; Corre, S; Gauthier, Gilles; Robert, Rene; Ansquer, Dominique.
Scallop Pecten maximus larvae have been cultured at the Argenton and Tinduff (Brittany, France) hatcheries with antibiotic treatment (chloramphenicol at 8 ppm) for 15 yr. Without treatment, outbreak of disease has normally occurred between Day 12 and Day 19 or sometimes earlier. A bacteriological study of larvae reared with and without antibiotic was performed over a 4 yr period. Among the collected strains, 2 clusters (C and F) of vibrios were present at high densities only in larvae cultured without treatment. One cluster (C) was routinely isolated over the 4 yr of study, while the other (F) was collected only in the third year. Their virulence with respect to scallop larvae and their lack of infectivity with respect to oyster larvae were demonstrated in...
Tipo: Text
Palavras-chave: Larvae; Bivalve; Vibrio; Scallop.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00090/20077/17716.pdf

Prédation de la palourde japonaise Ruditapes philippinarum par le baliste Balistes capricus
Provedor de dados: 5
Autores: Robert, Rene; Artiguenave, F.; Parra, M.
1988 s’avérait être, pour le bassin d'Arcachon, l'année d'un réel départ de l’élevage de la palourde Japonaise Ruditapes phillipinarum. Cinq millions de naissains étaient ainsi ensemencés, principalement au printemps, soit 4 a 5 fois plus que les années antérieures. La récolte théorique aurait dû avoisiner 50 tonnes en 1990, soit 1/10 de la production nationale . Or d'importantes mortalités étaient signalées au cours de l’été 1988, 80% environ du cheptel ensemencé ayant été touché.
Tipo: Text
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00090/20161/17814.pdf

Effect of the culture system and culture technique on biochemical characteristics of Pavlova lutheri and its nutritional value for Crassostrea gigas larvae
Provedor de dados: 5
Autores: Ponis, Emanuele; Parisi, G; Le Coz, Jean-rene; Robert, Rene; Zittelli, G; Tredici, R.
Pavlova lutheri was cultivated in carboys using batch technique and in a flat alveolar photobioreactor (FAP), using initially batch and then semi-continuous techniques. Growth and productivity of the cultures were compared and their nutritional quality was assessed by analysing their biochemical composition (gross composition, fatty acids and sterols) and by evaluating their food value for Pacific oyster (Crassostrea gigas) larvae as bispecific diet in association with Chaetoceros calcitrans forma pumilum. The highest productivity was achieved with the FAP operating in semi-continuous mode. Gross composition of P. lutheri was similar in all cultures, while cellular organic matter content of the microalga grown in FAP in semi-continuous mode was lower....
Tipo: Text
Palavras-chave: Semi continuous culture; Pavlova lutheri; Flat alveolar photobioreactors; Crassostrea gigas; Carboys; Biochemical composition; Batch culture.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-6117.pdf

Pathogenic threats and probiotic use in larviculture of the scallop, Pecten maximus
Provedor de dados: 5
Autores: Kesarcodi-watson, Aditya; Miner, Philippe; Nicolas, Jean-louis; Asmani, Katia; Robert, Rene.
During a series of experiments, bacteriological elements in scallop larval rearing were investigated: larvae susceptibility to pathogens as a function of their age, and the use of probiotic bacteria during larviculture. Younger larvae (d5 PF) were highly more susceptible to pathogenic-challenge than their older siblings, which were challenged at an older age (d15 PF). A challenge with 104 CFU mL−1 of V. pectenicida killed 100% of d5 PF larvae 7 days following challenge, yet killed only 9% of d15 PF larvae 9 days following challenge. Use of the probiotics Phaeobacter gallaeciensis, Alteromonas macleodii 0444 and Neptunomonas sp. 0536, provided for larger larvae, a high yield of competent larvae and, perhaps more importantly, protection against...
Tipo: Text
Palavras-chave: Scallop; Pecten maximus; Probiotics; Alteromonas macleodii; Neptunomonas; Phaeobacter.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00210/32153/30900.pdf

Action de l'acétate de tributyle-étain sur les oeufs et larve D de deux mollusques d'intérêt commercial : Crassostrea gigas (Thunberg) et Mytilus galloprovincialis (Lmk)
Provedor de dados: 5
Autores: Robert, Rene; His, Edouard.
L'acétate de tributyle-étain, qui entre dans la composition de certaines peintures antisalissures, est fortement toxique pour les oeufs et les larves D de Crassostrea gigas a la teneur de 5 p.p.b. (HIS et. ROBERT, 1980). Des concentrations inférieures, aussi basses que 1 microgramme par litre, ont été testées sur cette même espèce, mais aussi sur la moule, Mytilus galloprovincialis.
Tipo: Text
Ano: 1981
URL: http://archimer.ifremer.fr/doc/00091/20268/17912.pdf

Rearing of scallops (Pecten maximus) in France, from hatchery to intermediate culture, results of a 10 year programme (1983-1993)
Provedor de dados: 5
Autores: Dao, Jean-claude; Barret, Jean; Devauchelle, Nicole; Fleury, Pierre-gildas; Robert, Rene.
A stock enhancement programmr for scallop was initiated in France in 1983. As the natural spat collection on the french fishing grounds as well on Irish and Scottish waters was unsuccessful. (...)
Tipo: Text
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00091/20263/17908.pdf

Élevage de la palourde japonaise Ruditapes philippinarum dans le bassin d'Arcachon. Bilan des dix dernières années et  perspectives de développement
Provedor de dados: 5
Autores: Robert, Rene; Deltreil, Jean-pierre.
Farming of the Japanese clam Ruditapes philippinarum took place for the first time in 1980 in the Arcachon basin. After an experimentation period of about ten years, a farming strategy, taking into consideration the distinctive features found in the bay, was able to emerge. For example, in order to fight against clam predators, crabs such as Carcinus maenas, and balistes such as Balistes capriscus, vertical protection coupled with prior ground gravelling, and laying of nets over the beds during the summer months, have become necessary. The best raising technique seems to rest on early spring planting of middle size spats (T5), after checking that no brown rings are visible. If clam growth seems to be better in oceanic zones, it looks similar in other areas...
Tipo: Text
Palavras-chave: Basin of Arcachon; Culture methods; Growth; Predation; Balistes capriscus; Carcinus maenas; Ruditapes philippinarum; Bassin d'Arcachon; Stratégie d'élevage; Croissance; Prédation; Balistes capriscus; Carcinus maenas; Ruditapes philippinarum.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/rapport-1652.pdf

Effect of nutrition on Crassostrea gigas larval development and the evolution of physiological indices Part B: Effects of temporary food deprivation
Provedor de dados: 5
Autores: Ben Kheder, Rym; Quere, Claudie; Moal, Jeanne; Robert, Rene.
In the natural environment bivalve larvae are exposed to variable conditions and can therefore face periods of food shortage. To understand the resistance of early life stages of Pacific oyster (Crassostrea gigas), larvae were experimentally starved for 4 days at different periods of their development, and the resulting variations in their lipid reserves then analysed using image analysis and biochemical techniques. Faced with a temporary lack of food, the larvae halted their growth in size and weight and started to live off their lipid reserves; this effect was highly marked when the period of food deprivation was at the beginning of larval rearing, but was less marked when food deprivation was after day 14. The larvae conserved their developmental...
Tipo: Text
Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Starvation; Lipids.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00018/12906/10145.pdf

Croissance et spectre de taille de trois algues en cultures non renouvelées, utilisées pour la nutrition de larves de bivalves en écloserie
Provedor de dados: 5
Autores: Robert, Rene; His, Edouard.
The different growth stages of three marine unicellular algae used in aquaculture, Isochrysis galbana, Chaetoceros calcitrans forma pumilum and tetraselmis suecica have been studied for 50 days under controlled culture conditions using a particle meter. At the same time, the size spectrum of the cells has been determined using a C 1000 scanner. The exponential growth stage is 11 days in the first two species, and 13 in the last one. Chaetoceros calcitrans forma pumilum is the smallest with an average size of 3.5 µm. Its cellular volume is constant. The average size of Isochrysis galbana is 4.5 µm. An increase of the cellular volume is observed through its stationary stage. The modal size of Tetraselmis suecica is 8 µm.  Again, an increase in the cellular...
Tipo: Text
Palavras-chave: Spectre de taille; Croissance; Tetraselmis suecica; Chaetoceros calcitrans forma pumilum; Isochrysis galbana; Algues unicellulaires.
Ano: 1986
URL: http://archimer.ifremer.fr/doc/1986/acte-6187.PDF

Toxicité d'un désherbant, l'atrazine-simazine, sur les jeunes stades larvaires de Crassostrea gigas et sur deux algues fourrages, Isochrysis aff-galbana et Chaestoceros calcitrans
Provedor de dados: 5
Autores: Robert, Rene; His, Edouard; Maurer, Daniele.
Corn culture has strongly developed in Aquitaine (France) over the past years, and lead to an increased use of pesticides and herbicides. One of the most commonly used formulations around the coast of the Arcachon Bay, an historical oyster spat capture zone, is the Atrazine-Simazine mix. The action limits of this product are defined on Crassostrea gigas larvae and on two nanoplankton test strains used in a controlled environment for their food, Isochrysis aff-galbana and Chaetoceros calcitrans. Herbicides can indeed directly impact on oyster larvae, but they can also have a negative impact by interfering with the development of the algae used as aquaculture feed and feed for veligeres.
Tipo: Text
Palavras-chave: Chaestoceros calcitrans; Isochrysis aff galbana; Algues unicellulaires; Crassostrea gigas; LARVES; Simazine; Atrazine; Ecotoxicologie.
Ano: 1985
URL: http://archimer.ifremer.fr/doc/1985/acte-6183.PDF

Amélioration des productions phytoplanctoniques en écloserie de mollusques : caractérisation des microalgues fourrage
Provedor de dados: 5
Autores: Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie.
in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the...
Tipo: Text
Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf

Action de l'acétate de tributyle-étain sur les oeufs et les larves D de deux mollusques d'intérêt commercial : Crassostrea gigas (Thunberg) et Mytilus galloprovincialis (Lmk)
Provedor de dados: 5
Autores: Robert, Rene; His, Edouard.
The Tributyl tin acetate, a component of certain antifouling paints, has deleterious effects on eggs and D shaped larvae from Crassostrea gigas at 5 p.p.b. (HIS &amp; ROBERT, 1980). Here we have tested concentrations as low as 1 p.p.b. on eggs and on 24 hours old larvae both from Crassostrea gigas and Mytilus galloprovincialis. The same results are got ; this product kills embryos and larvae in a few days ; it prevents growth of 24 hours old larvae exposed to this toxic. Mussels larvae seem to be less sensitive.
Tipo: Text
Palavras-chave: Antifouling; Larves; Pollution; Toxicité; Bivalvia; Crassostrea gigas; Mytilus galloprovincialis; Antifouling; Larvae; Pollution; Toxicity; Bivalvia; Crassostrea gigas; Mytilus galloprovincialis.
Ano: 1981
URL: http://archimer.ifremer.fr/doc/00037/14862/12185.pdf

A Dynamic Energy Budget (DEB) growth model for Pacific oyster larvae, Crassostrea gigas
Provedor de dados: 5
Autores: Rico-villa, Benjamin; Bernard, Ismael; Robert, Rene; Pouvreau, Stephane.
Tipo: Text
Palavras-chave: Dynamic Energy Budget; Crassostrea gigas; Bivalve larvae; Ecophysiology; Growth.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00009/11996/9040.pdf

Bilan des essais d'élevage du pétoncle noir, Chlamys vria (Linné) dans le Bassin d'Arcachon France
Provedor de dados: 5
Autores: Robert, Rene; Trut, Gilles; Laborde, Jean-louis; Miner, Philippe.
Until 1960, the black scallop Chlamys varia (Linné, 1758) was largely distributed over the French Atlantic coast. Nowadays, most of the deposits are gone. Since controlled mollusc reproduction techniques are now relatively mastered, some reimplatation attempts have been conducted on a number of oyster culture sites, including the Arcachon Bay, from 1989 to 1992. Despite some good results in hatchery and nursery, the rearing attempts failed. If some good growth rates have been monitored in the Arcachon Bay over these three years, these performances have been frustrated by very high mortality rates occurring shortly after the setting up of the farms. Although this species is known to be relatively sensitive to environmental factors, the hydrobiological...
Tipo: Text
Palavras-chave: Bassin Arcachon; Elevage; Chlamys varia; Pétoncle.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-6193.PDF

Lethal and sub-lethal effects of Deepwater Horizon slick oil and dispersant on oyster (Crassostrea virginica) larvae
Provedor de dados: 5
Autores: Vignier, J.; Soudant, P.; Chu, F. L. E.; Morris, J. M.; Carney, M. W.; Lay, C. R.; Krasnec, M. O.; Robert, Rene; Volety, A. K.
In April 2010, crude oil was spilled from the Deepwater Horizon (DWH) oil platform for 87 days, coincident with the spawning season and recruitment of the oyster, Crassostrea virginica, in the Gulf of Mexico. Impacts of acute exposures to surface-collected DWH oil (HEWAF), dispersed oil (CEWAF) and dispersant alone (Corexit 9500A®) on planktonic larval stages of C. virginica (veliger, umbo and pediveliger) were tested in the laboratory. Exposures to HEWAF, CEWAF and dispersant were toxic to larvae impairing growth, settlement success and ultimately survival. Larval growth and settlement were reduced at concentrations of tPAH50 ranging from 1.7 to 106 μg L−1 for HEWAF and 1.1–35 μg L−1 for CEWAF, concentrations well within the range of water sampled during...
Tipo: Text
Palavras-chave: Deepwater Horizon oil spill; Crassostrea virginica; Corexit 9500A (R); Larvae; Growth; Settlement; PAH.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00345/45572/45181.pdf

Interactions between Crassostrea virginica larvae and Deepwater Horizon oil: Toxic effects via dietary exposure
Provedor de dados: 5
Autores: Vignier, J.; Rolton, A.; Soudant, Philippe; Chu, F. L. E.; Robert, Rene; Volety, A. K.
The Deepwater Horizon (DWH) disaster released crude oil in the Gulf of Mexico for 87 days, overlapping with the reproductive season and recruitment of the oyster Crassostrea virginica. The pelagic larval life stages of C. virginica are particularly vulnerable to contaminants such as polycyclic aromatic hydrocarbons (PAHs) and oil droplets. Based on their lipophilic properties, PAHs and oil droplets can adsorb onto phytoplankton and filter-feeding C. virginica larvae may be exposed to these contaminants bound to suspended sediment, adsorbed onto algal and other particles, or in solution. This study examined the effects of exposure of C. virginica larvae to algae mixed with DWH oil. In a 14-day laboratory exposure, 5 day-old C. virginica larvae were exposed...
Tipo: Text
Palavras-chave: Deepwater Horizon oil; Droplet; Oyster larvae; Dietary exposure; PAH.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00473/58423/61445.pdf

The combined effects of blue light and dilution rate on lipid class and fatty acid composition of Tisochrysis lutea
Provedor de dados: 5
Autores: Marchetti, Julie; Da Costa, Fiz; Bougaran, Gael; Quere, Claudie; Soudant, Philippe; Robert, Rene.
The lipid class and the fatty acid compositions of microalgae highly influence bivalve larval and post-larval development. Light is an essential environmental factor for microalgal culture, and quantity and quality of light may induce changes in the biochemical composition of the algae. The objective of this study was to investigate the effect of light spectrum (blue vs. white light) on lipid class and fatty acid compositions of Tisochrysis lutea cultured in a chemostat. Two different dilution rates (D) were assayed for each light spectrum: 0.2 and 0.7 day−1. Triacylglycerol (TAG), sterol, and hydrocarbon (HC) content increased sharply at low D. The proportion of alkenones was significantly reduced under blue light. Polyunsaturated fatty acids (PUFA), and...
Tipo: Text
Palavras-chave: Tisochrysis lutea; Blue light; Dilution rate; Lipid classes; Fatty acids; Chemostat.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00412/52299/53355.pdf

Observations complementaires sur les causes possibles des anomalies de la reproduction de Crassostrea gigas (Thunberg) dans le bassin d'Arcachon.
Provedor de dados: 5
Autores: His, Edouard; Maurer, Daniele; Robert, Rene.
The spat production in the Bay of Arcachon has been recently disturbed. Bioassays using algae used as food for oyster larvae have shown that the organotin paints affect or inhibit growth of Isochrysis galbana and Chaetoceros calcitrans . Fresh waters coming into the bay and shore sediment did not affect the growth of these algae. Bioassays proposed by Woelke (1967) using embryos and larvae of Crassostrea gigas have shown that the quality of the sea water from Arcachon harbour has been improved a few months after the use of organotin paints has been prohibited. Likewise fresh waters are free from any micropollutants that could drastically disturb the reproduction of C. gigas in the bay.
Tipo: Text
Palavras-chave: Marine mollusks; Pollution effects; Recruitment; Population number; Oyster culture.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/publication-1788.pdf

Larval development and fatty acid composition of Ostrea edulis (L.) fed four different single diets from conditioning to pre-settlement
Provedor de dados: 5
Autores: Gonzalez-araya, Ricardo; Robert, Rene.
Survival, growth and fatty acid composition of Ostrea edulis larvae (L.) fed four different single species, microalgal diets, Tisochrysis lutea (T), Chaetoceros neogracile (Cg), Skeletonema marinoi (Sm) or Tetraselmis suecica (Ts) from broodstock to pre-settlement, were studied. Lower larval growth (5.5m to 6.5m/d) was recorded in progeny continuously fed single S.marinoi or T.suecica, whereas good growth was achieved with single T.lutea (7.8m/d). Larvae, originated from broodstock receiving Sm or Ts, exhibited growth compensation when fed a bispecific balanced diet (TCg). This did not occur when broodstock and larvae were fed Cg or T, for which single or mixed diets led to similar larval growth. Furthermore, survival was high (&gt;90%) regardless of...
Tipo: Text
Palavras-chave: Broodstock conditioning; Diet; Larval development; Ostrea edulis.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00438/54986/56483.pdf

Influence of sole and plurispecific diets on larval consumption and development of Pacific oyster Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Robert, Rene; Rico Villa, Benjamin; Mingant, Christian.
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-6695.pdf

Sensitivity of eastern oyster ( Crassostrea virginica) spermatozoa and oocytes to dispersed oil: Cellular responses and impacts on fertilization and embryogenesis
Provedor de dados: 5
Autores: Vignier, J.; Volety, A. K.; Rolton, A.; Le Goic, N.; Chu, F. -l. E.; Robert, Rene; Soudant, P.
The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF. Following a 30-min exposure, the number of spermatozoa and their viability were...
Tipo: Text
Palavras-chave: Deepwater Horizon oil spill; Oyster; Spermatozoa; Oocyte; Flow-cytometry.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00377/48851/51168.pdf

Estimation de la teneur en acétate de tributyle étain dans l'eau de mer, par une méthode biologique
Provedor de dados: 5
Autores: His, Edouard; Maurer, Daniele; Robert, Rene.
L’acétate de tributyle-étain, composé actif de certaines peintures antisalissures, représente un danger certain pour les zones conchylicoles de captage (E. HIS et R. ROBERT, 1980 et R. ROBERT et E. HIS, 1981). Les méthodes chimiques actuelles ne sont pas assez sensibles pour permettre le dosage de ce produit dans l'eau de mer, au seuil de toxicité pour les larves d'huitres. Une échelle d'action pour douze concentrations comprises entre 100 ug/l. et 0,02 ug/ l., sur les véligères de Crassostrea gigas est proposée. Elle permet d’évaluer le degré de contamination de l'eau de mer d'un part de plaisance et son évolution.
Tipo: Text
Ano: 1983
URL: http://archimer.ifremer.fr/doc/00090/20162/17815.pdf

A physiological and biochemical approach to selecting the ideal diet for Ostrea edulis (L.) broodstock conditioning (part A)
Provedor de dados: 5
Autores: Gonzalez-araya, Ricardo; Queau, Isabelle; Quere, Claudie; Moal, Jeanne; Robert, Rene.
To select the best microalgae for Ostrea edulis conditioning, four single species diets were fed to batches of broodstock, which were then compared using physiological and biochemical approaches. Ingestion and absorption were first studied, showing that Chaetoceros gracilis and Skeletonema marinoï were efficiently ingested (4.9–5.3 mg g−1 h−1) and absorbed (1.9–2.5 mg g−1 h−1) while Tetraselmis suecica led to the lowest physiological responses (0.36 and 0.12 mg g−1 h−1 respectively). When O. edulis were fed any microalgae except T-ISO with only 79 mg g−1, gonads accumulated carbohydrates from 116 to 141 mg g−1 and the extent of accumulation depended on the species supplied in the order T. suecica&lt;C. gracilis&lt;S. marinoï. When oysters were fed either...
Tipo: Text
Palavras-chave: Ostrea edulis; Conditioning; Algal diets; Ingestion; Absorption; Biochemical composition.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14449/11748.pdf

Croissance et spectre de tailles de six algues utilisees pour la nutrition de larves de bivalves en ecloserie, en culture non renouvelee
Provedor de dados: 5
Autores: Robert, Rene; His, Edouard.
The growth and the size-frequency distribution of six unicellular marine algae, Isochrysis aff. galbana "Tahiti" nom. prov., Pavlova lutheri (Droop) Green, Pseudoisochrysis paradoxa nom. prov., Tetraselmis tetrathele (G.S. West) Butcher, Chaetoceros calcitrans (Paulsen) Takano et Chaetoceros gracilis Schutt have been studied. Most of these species showed an increase in size during the stationary phase of growth. Because these data and anterior ones were achieved in similar experimental conditions, they were ranked by size and their growth performances discussed.
Tipo: Text
Palavras-chave: Molluscan larvae; Hatcheries; Nutrition; Growth; Algal culture.
Ano: 1985
URL: http://archimer.ifremer.fr/doc/1985/publication-1778.pdf

Effect of nutrition on Crassostrea gigas larval development and the evolution of physiological indices. Part A: Quantitative and qualitative diet effects
Provedor de dados: 5
Autores: Ben Kheder, Rym; Quere, Claudie; Moal, Jeanne; Robert, Rene.
Tipo: Text
Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Nutrition level and quality; Lipids; Condition indices.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00009/11997/9041.pdf

Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis.
This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5‰ in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 105 ml− 1) and in larvae (103 larva− 1) on day 15.  Bacterial...
Tipo: Text
Palavras-chave: RAS; Bivalve larvae; Microbiota; Pyrosequencing; Hatchery.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00346/45691/45311.pdf

Principaux aspects de la dynamique d'une population d'Ophiura texturata en Baie de Douarnenez
Provedor de dados: 5
Autores: Guillou, J.; Robert, Rene.
Sampling of Ophiura texturata was carried out during a two year cycle . Demographic structure is obtained using biometric method. The main parameter s of the population are computed . Plurimodal year classes are followed, and the periods of spawning and recruitment are defined . Bathymetric distribution taking into account the seasonal fluctuation is exposed. Segregation of the year classes and cohorts, and variations of their densities are demonstrated.
Tipo: Text
Ano: 1979
URL: http://archimer.ifremer.fr/doc/00090/20160/17813.pdf

Valeur alimentaire de Tetraselmis striata et T. chui pour les larves de Crassostrea gigas
Provedor de dados: 5
Autores: Ponis, Emanuele; Parisi, Guilina; Robert, Rene.
In an attempt to simplify mollusc hatchery-nursery procedures and to provide cost-effective alternative diets, the use of concentrated and preserved microalgae is an alternative to the use of fresh microalgae. Because of their ability for preservation, Prasinophytes appears to be good candidates for remote algal production. In the present work, the food value of fresh and preserved Tetraselmis chui and T. striata for umboned Crassostrea gigas larvae was studied. During the first experiment, fresh T. striata led to the best larval development, but these performances were not confirmed subsequently. When used as monospecific diets the food value of both Prasinophytes is unsufficient to support a good C. gigas larval development. However in bispecific diets a...
Tipo: Text
Palavras-chave: Food value; Microalgae; Crassostrea gigas; Tetraselmis chui; Tetraselmis striata; Valeur alimentaire; Microalgues; Crassostrea gigas; Tetraselmis chui; Tetraselmis striata.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-6203.PDF