Consulta: subjectFacets:"Antibodies"
Registros recuperados: 47
Data/hora: 06/06/2024 01:04:52

Importância do colostro na transmissão da imunidade passiva em caprinos.
Provedor de dados: 14
Autores: ELOY, A. M. X.
bitstream/item/92545/1/cot-133.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Imunidade passiva; Raça Saanen; Raça Moxotó; Immunological factors; Kids.; Caprino; Colostro; Cabrito; Anticorpo; Imunologia; Goats; Antibodies; Passive immunity.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/971211

Desenvolvimento de uma prova de imunoadsorcao enzimática para detecção de anticorpos contra Trypanosoma vivax em bovinos: resultados preliminares.
Provedor de dados: 14
Autores: MADRUGA, C. R.; ARAUJO, F. R.; CRUZ, T. M. da; SCHENK, M. A. M.
bitstream/item/137691/1/PESQ-EM-ANDAMENTO-50.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Mato Grosso; Mato Grosso do Sul; Brasil.; Bovino; Anticorpo; Elisa; Trypanosoma Vivax.; Antibodies; Brazil; Cattle.
Ano: 1999
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/322104

Teste ELISA para detectar anticorpos contra as toxinas "C" e "D" de clostridium botulinum em bovinos.
Provedor de dados: 14
Autores: MADRUGA, C. R.; QUEIROZ, R. A.; PIRES, P. P.; MORAES, S. da S.
bitstream/item/132393/1/COT-64.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Anticorpo; Botulismo; Bovino; Clostridium Botulinum; Elisa.; Antibodies; Cattle.; Botulism.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/323911

Effects de le temperature et du regime alimentaire sur certains parametres de l'immunite cellulaire du loup de mer en elevage
Provedor de dados: 5
Autores: Deschaux, P; Peres, G.
The data showed that antibody response and NK cells activity of a teleostean fish Dicentrarchus labrax (Loup de mer) were maximal when animals were bred at a temperature equal to 18 degree C. A proteinoprive diet enhanced T cytotoxic response and diminished NK cell activity. A fast of 15 days had no effect on these two cellular responses.
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Serranidae; Pisces; Antibodies; Starvation; Toxicity; Cells; Immunity; Diets; Nutrition; Temperature effects; Fish culture.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1248.pdf

Fixation d''un anticorps anti-lysozyme dans des cellules de mollusques bivalves
Provedor de dados: 5
Autores: Auffret, Mathieu; Diouris, M; Herry, A.
A polyclonal rabbit anti-human lysozyme (Dako) was applied on histological sections from bivalve mollusc and revealed with a mouse anti-rabbit IgG fluorescein conjugated antibody to localise cellular lysozyme in tissues where its activity had previously detected by enzymatic techniques. Labelling occurred in the cytoplasm of epithelial cells covering the body. This is in agreement with the intracellular distribution of this enzyme. In the Lucinid Lucinella divaricata gills, the cytoplasm of bacteriocytes was also labelled. This suggests that antibacterial activity occurred in these cells. Control sections indicate that fluorescence was a consequence of true primary antibody fixation. However, its specificity should be verified by electrophoretical...
Tipo: Text
Palavras-chave: Bivalvia; Immunology; Antibodies; Electrophoresis; Histology.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-1723.pdf

TOXIC SHOCK SYNDROME
Provedor de dados: 79
Autores: BERGDOLL,M. S.
Toxic shock syndrome is a staphylococcal disease caused by toxins produced by the staphylococci, toxic shock syndrome toxin-1 and enterotoxin B. The disease results from staphylococci growing in the vagina with the use of tampons during menstruation, primarily in young women. However, any staphylococcal infection can result in toxic shock syndrome if the staphylococci produce the appropriate toxins and the individual has no antibodies to the toxins. The symptoms can be quite severe, with high fever, low blood pressure, diffuse macular erythroderma, orthostatic dizziness, vomiting and or diarrhea at the onset, severe myalgia, peeling of the skin of the palms of the hands and the soles of the feet after seven to ten days, and death in some cases. The disease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxic shock syndrome; Toxic shock syndrome toxin; Staphylococci; Enterotoxins; Antibodies.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000100002

In vitro assay of biological and chemical toxins using antibodies against lethal toxin neutralizing factor
Provedor de dados: 79
Autores: LIPPS,B. V.
Lethal Toxin Neutralizing Factor (N-LTNF), MW 63.0 kDa, was isolated from opossum serum. After trypsin digestion, the active domain of N-LTNF was isolated and sequenced. The synthetic peptide consisting of ten amino acids was designated as LT-10. N-LTNF and LT-10 inhibited the lethality of animal, plant and bacteria toxins when tested on mice non-immunologically. The antibodies against N-LTNF and LT-10 only reacted immunologically with toxins and not with non-toxic substances. Anti-LTNF and anti-LT-10 reacted immunologically by ELISA test with toxins that were not detected by mouse test, such as cholera toxin and digoxin. Anti-LTNF and anti-LT-10 failed to react immunologically with non-toxic substances, such as nerve growth factor and collagen. Currently,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; In vitro assay; Lethal toxin neutralizing factor; Toxins; Venoms.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200003

USE OF AN ELISA ASSAY TO EVALUATE VENOM, ANTIVENOM, IgG AND IgM HUMAN ANTIBODY LEVELS IN SERUM AND CEREBROSPINAL FLUID FROM PATIENTS BITTEN BY Crotalus durissus terrificus IN BRAZIL
Provedor de dados: 79
Autores: BARRAVIERA,B.; SARTORI,A.; PEREIRA DA SILVA,M. F.; KANENO,R.; PERAÇOLI,M. T. S.
A sandwich-type ELISA technique for specific and sensitive detection of Crotalus durissus terrificus venom antigens, horse-antivenom, human IgG and IgM antibodies was set up. Sixteen patients, 13 males and 3 females aged between 13 to 63 years (mean 33 ± 15) bitten by Crotalus durissus terrificus snakes were studied. Of the 15 patients, 6 had previously received anti-Crotalus venom and no seric venom was detected. For the other 9 patients studied, the venom levels ranged from 2 to 108 ng/ml according to the severity of each case. Seric antivenom was detected up to 44 days after the bite. IgM human antibody levels against Crotalus venom were higher between 3 and 18 days after specific treatment. IgG human antibody levels against Crotalus venom were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme-linked immunosorbent assay; Crotalus durissus terrificus; Venom; Antivenom; Antibodies; Cerebrospinal fluid.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000100003

Leptospira spp. antibodies in captive coatis (Nasua nasua Storr, 1780) (Carnivora: Procyonidae)
Provedor de dados: 80
Autores: Langoni,H; Kawaguchi,MF; Oshika,JC; Da Silva,RC; Teixeira,CR.
Leptospirosis is a worldwide infection that affects many species, including wild animals. The present study aimed to detect Leptospira spp. antibodies in 17 captive coatis (Nasua nasua) by the microscopic agglutination test (MAT). Nine (52.94%) animals tested positive and the following serovars were identified: Copenhageni (22.22%), Shermani (22.22%), Andamana (11.11%), Wolfii (11.11%) and Pyrogenes (11.11%). Two samples presented coagglutination, one (11.11%) for Hebdomadis and Wolfii, and another (11.11%) for Hebdomadis, Hardjo and Wolfii. The current study revealed the presence of the infection even in animals without any clinical signs, reinforcing the possibility that wild animals in captivity may be infected by leptospire serovars, thus enabling...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nasua nasua; Leptospira spp.; Captive coatis; Antibodies; Microscopic agglutination test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400013

Detection of antibodies to Toxoplasma gondii in wild animals in Brazil
Provedor de dados: 80
Autores: Silva,Rodrigo Costa da; Machado,Gustavo Puglia; Cruvinel,Tatiane Morosini de Andrade; Cruvinel,Ciro Alexandre; Langoni,Helio.
Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008.Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Natural infection; Antibodies; Wild animals; MAT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200603

Parasitological and Serological Survey of Domestic Goats for Leishmaniosis in Baringo District, Kenya
Provedor de dados: 19
Autores: Robert, L. L.; Mbati, P. A.; Schaefer, K. U.; Shatry, A. M.; Anjili, C. O.; Githure, J. I.
Palavras-chave: Goat; Leishmania donovani; Reservoir; Antibodies.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/268

Diagnosis of infectious bronchitis: an overview of concepts and tools
Provedor de dados: 89
Autores: Villarreal,LYB.
Infectious bronchitis (IB) casues multi-systemic infection in chickens with signs similar caused by other poultry pathogens and thus a high diagnostic accuracy can only be achieved by s series of laboratory assays. This article reviews in a brief way the traditional virus assays such as embryo innoculation, tracheal rings and virus neutralization assays for the direct detection of Avian infectious bronchitis virus (IBV) and methods based on gene molecular biology and some assays for the detection of anti-IBV antibodies, including ELISA. A critical view on each technique is also provived by the author.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Diagnosis; IBV; Typing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200006

Development of an enzyme-linked immunosorbent assay for quantification of Salmonella enteritidis-specific antibodies in egg yolk
Provedor de dados: 89
Autores: Tayeb,IT; Nehme,P; Jaber,L; Barbour,EK.
The present study aims at developing an indirect ELISA to quantify yolk antibodies specific to all surface proteins of the invasive Salmonella Enteritidis (SE), which acquired the 1.8, 14.1, and ~ 50 Kb plasmids. An ELISA checkerboard was used in four different experiments to account for the different parameters included in the preliminary ELISA procedure, and consequently to maximize the difference in Optical Density (OD) values between control positive and negative yolk samples. The first experiment aimed at studying the impact of 5% Bovine Serum Albumin (BSA) dissolved in distilled water as a blocking reagent on a 28 µg/well SE antigen-coated plate, while applying the positive and negative control yolk samples to different concentrations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Egg yolk; ELISA; Salmonella Enteritidis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400009

Influence of glutamine and vitamin E on the performance and the immune responses of broiler chickens
Provedor de dados: 89
Autores: Sakamoto,MI; Murakami,AE; Silveira,TGV; Fernandes,JIM; Oliveira,CAL de.
This study aimed at evaluating the influence of Glutamine (Gln) and Vitamin E (VE) supplementation on the performance and immune response of broilers. A completely randomizes experimental design with a 2 x 3 (VE x Gln) factorial arrangement was used. VE was supplemented at 10 and 500 mg/kg feed, with or without Gln (1%) addition, and two periods of supplementation in the starter diets (1-7 and 1-14 days of age), with five replicates of 50 birds each. The analyzed parameters were: live performance (weight gain, feed intake, and feed conversion ratio); relative weights of the spleen, bursa, and thymus; antibody titers (with sheep red blood cells suspension - SRBC) and cutaneous basophilic hypersensivity (CBH). Data were submitted to the analysis of variance,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Sheep red blood cells; Cutaneous basophilic hypersensitivity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400007

Effect of bee pollen on the immunity and tibia characteristics in broilers
Provedor de dados: 89
Autores: Oliveira,MC de; Silva,DM da; Loch,FC; Martins,PC; Dias,DMB; Simon,GA.
This study was carried out to evaluate the effect of bee pollen (BP) levels on the IgG and IgM titers, weight of lymphoid organs, and on the tibia morphometric measures and mineralization in broilers at 21 and 42 days of age. Four hundred birds were used in an entirely randomized design with four treatments (0, 0.5, 1 and 1.5% of BP feed inclusion) and five replicates. At 21 and 42 days of rearing, blood samples were collected for IgG and IgM analysis, as well as lymphoid organs (bursa, thymus and spleen) and the tibiae. There was no effect (p&gt;0.05) of the BP inclusion on IgG titers, bursa and spleen weights, tibia morphometric measures and mineralization at 21 and 42 days, IgM titer at 42 days or thymus weight at 21 days. However, IgM titers at 21 days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Bee products; Lymphoid organs.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000400006

Colostral immunoglobulins absorption in Canchim and Nelore calves
Provedor de dados: 96
Autores: Machado Neto,Raul; Packer,Irineu Umberto; Prado,Gladys Villas Boas do; Bessi,Rosana; Pauletti,Patricia.
The efficiency of absorption of colostral immunoglobulins was evaluated in five Canchim and seven Nelore calves. They received colostrum pools with concentration of 70.20 ± 6.14 mg/mL through esofageal feeder at 2, 12, 24 and 36 hours after birth. The immunoglobulins concentrations of the pools were estimated through specific gravity and measured by radial immunodifusion. In the blood collection at birth and during the first 70 days of life, the total protein was assayed by biuret method and the immunoglobulins were assayed by radial immunodifusion. Data were analysed as a randomized split-plot statistical model. The highest concentrations of serum immunoglobulins and total protein were observed at 24 hours of age. No significant differences (P&gt;0.5484)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Colostrum; Newborn calves; Passive immunity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000600021

HUMORAL RESPONSE TO DIFFERENT VACCINATION SCHEMES AGAINST CLASSICAL SWINE FEVER (CSF) SUCCESSIVELY APPLIED DURING AN OUTBREAK OF THE DISEASE
Provedor de dados: 101
Autores: Percedo,María Irian; Alfonso,P; Frías,María Teresa; Díaz de Arce,Heydi; Barrera,Maritza; Fonseca,O; Castell,Sara.
Vaccination against classical swine fever (CSF) is an essential measure for its control in endemic areas, and the Chinese Strain has been used with success in already disease free countries. Nevertheless the complexity of the factors interacting on the epidemiology and control of CSF, when the expected success is not reached with the vaccination of premises, doubts always fall on the vaccine used and the vaccination scheme applied In consecuences, many changes in vaccination scheme by the breeders are frecuently observed whitout positive influence in CSF control. To clarify the CSF recurrence in a herd vaccinated by a different scheme as the one recommended in the national control program, serological samples were taken to different animal groups in three...
Tipo: Journal article
Palavras-chave: Classical swine fever; Antibodies; Vaccination; Swine; Serology; Focus.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000300004

Quality of colostral passive immunity and pattern of serum protein fluctuation in newborn calves
Provedor de dados: 63
Autores: Pauletti,Patricia; Machado Neto,Raul; Packer,Irineu Umberto; D'Arce,Raul Dantas; Bessi,Rosana.
Immunity acquired by newborn animals is known as passive immunity, and for ruminants, antibody acquisition depends on the ingestion and absorption of adequate amounts of immunoglobulins from colostrum. This study relates different initial levels of acquired passive protection and serum total protein (TP) and immunoglobulin G (IgG). Serum immunoglobulin concentration and total protein were evaluated for female Holstein calves in the first sixty days of life. Animals were separated into three groups according to their initial level of passive immunity: group 1- animals with a low level of passive immunity (below 20 mg mL-1); group 2- animals with a medium level (between 20 and 30 mg mL-1), and group 3- animals with a high level (above 30 mg mL-1). Serum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colostrum; Dairy calves; Antibodies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000300006

Expression of Grapevine leafroll-associated virus 3 coat protein gene in Escherichia coli and production of polyclonal antibodies
Provedor de dados: 102
Autores: Fajardo,Thor V.M.; Barros,Danielle R.; Nickel,Osmar; Kuhn,Gilmar B.; Zerbini,F. Murilo.
Grapevine leafroll-associated virus 3 (GLRaV-3), the main viral species of the grapevine leafroll complex, causes yield and quality reduction in grapes (Vitis spp.). The coat protein gene was RT-PCR-amplified from total RNA extracted from infected grapevine leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pRSET-C expression vector. The recombinant plasmid was used to transform Escherichia coli BL21:DE3 and express the capsid protein. The coat protein, fused to a 6 His-tag, was purified by affinity chromatography using an Ni-NTA resin. The identity of the purified protein was confirmed by SDS-PAGE and Western blot. The in vitro-expressed protein was quantified and used for rabbit...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Grapevine; GLRaV-3; Recombinant protein; Serology; Antibodies.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000600007

Accuracy of immunoglobulin M and immunoglobulin A of saliva in early diagnosis of dengue: Systematic Review and Meta-analysis
Provedor de dados: 42
Autores: COLONETTI,TAMY; ROCHA,BELISE V.E.; GRANDE,ANTÔNIO J.; ALEXANDRE,MARIA C.M.; DONDOSSOLA,EDUARDO R.; MADEIRA,KRISTIAN; ROSA,MARIA I.
ABSTRACT The objective of the study was to conduct a systematic review to synthesize the current evidence on the accuracy of IgM and IgA to early diagnosis the dengue virus. The review protocol was registered at PROSPERO (CRD 42015024808). We searched for studies in the following electronic database from 1990 to January 2018. The search identified 3507 studies. Five studies were included for quantitative analysis. Three studies included evaluations of salivary IgM provided a sensitivity of 86% and specificity of 93%. Two studies included evaluating of IgA salivary showed a combined sensitivity of 69% and a combined specificity of 98%. Despite the results found and the low methodological quality of the studies included in the meta-analysis it is still soon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunoglobulin M; Immunoglobulin A; Antibodies; Dengue virus; Diagnosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603147

Antibodies against pseudorabies virus in feral swine in southeast Brazil
Provedor de dados: 45
Autores: Cunha,E.M.S.; Nassar,A.F.C.; Lara,M.C.C.S.H.; Bersano,J.G.; Villalobos,E.M.C.; Oliveira,J.C.F.
Serum samples collected from 358 wild boars (Sus scrofa) in breeding farms in São Paulo, southeast Brazil, from 1998 to 2001, were tested for antibodies against pseudorabies virus (PRV) by means of serum neutralization (SN) and enzyme-linked immunobsorbent assay (ELISA). Seropositive animals were detected in three of seven herds analyzed. Overall seroprevalence as assessed by SN was 30.7%, ranging from 25.2% to 100% for the herds that presented seropositive animals. Indirect ELISA detected lower seroprevalence (19.3%). Sensitivity and specificity of ELISA were equal to 57.3% and 97.6%, respectively. Agreement was equal to 85.2% (P&lt;0.0001). These results showed that PRV infections occurred in farmed feral swine in southeast Brazil, and affect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wild boar; Sus scrofa; Pseudorabies virus; Aujeszky’s disease virus; Antibodies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400002

Ocular alterations in dogs naturally infected by Leishmania (Leishmania) chagasi
Provedor de dados: 45
Autores: Brito,F.L.C.; Alves,L.C.; Maia,F.C.L.; Santos,E.S.C.; Laus,J.L.; Meunier,I.M.J.
Ocular conditions, anti-Leishmania antibodies and total protein of the aqueous humor were studied in dogs naturally infected by Leishmania (Leishmania) chagasi. Fifty dogs were analyzed and assigned into two groups of 25 animals each. All animals were submitted to routine ophthalmic exam. Results showed that 76% of the affected animals presented ocular signs, being uveitis the predominant. The mean of total protein in the aqueous humor of animals with uveitis was higher (P&lt;0.05) when compared to the mean of animals with other ocular signs or no clinical sign. The anti-Leishmania chagasi antibody values in plasma were superior to those found in the aqueous humor (P&lt;0.05).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Leishmania (Leishmania) chagasi; Aqueous humor; Antibodies; Protein.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500011

Serological diagnosis of Babesia equi and B. caballi in pregnant mares
Provedor de dados: 45
Autores: Passos,L.M.F.; Ribeiro,M.F.B.; Anderegg,P.I.; Böse,R.
O objetivo do presente estudo foi determinar a dinâmica da produção de anticorpos anti-Babesia equi e B. caballi em éguas gestantes em condições naturais de campo em Minas Gerais e avaliar a eficiência da transferência passiva de anticorpos via colostro. Foram analisadas, pela imunofluorescência indireta, amostras de soros colhidas de 34 fêmeas aos 290, 305 e 320 dias de gestação, e no momento do parto, e de seus respectivos potros até 36 horas após o nascimento. Aos 290 dias de gestação, todas as fêmeas estavam positivas para B. equi e apenas duas estavam negativas para B. caballi. Todos os potros tornaram-se positivos para B. equi após ingestão de colostro, entretanto 42% permaneceram negativos para B. caballi 36 horas após o nascimento. Não se observou...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Babesia equi; Babesia caballi; Pregnant mares; Antibodies.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000600003

Production of specific polyclonal antibodies anti-human factor IX
Provedor de dados: 52
Autores: Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos.
In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010

Production of rabbit antibodies against purified Glucose oxidase
Provedor de dados: 52
Autores: Zia,Muhammad Anjum; Ain,Qurat-ul; Iftikhar,Tehreema; Abbas,Rao Zahid; Rahman,Khalil-ur.
Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg -1 specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) resulted into 22.53 U mg-1 specific activity and 10.27 fold purification. This was applied to sephadex G-200 column for gel filtration chromatography. It was observed that enzyme achieved 59.37 U mg-1of specific activity with 27.08 fold purity and 64.36% recovery. Purified glucose oxidase was injected into rabbits through intravenous route, to raise the glucose oxidase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose oxidase; A. niger; Antibodies; Rabbits.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100008

Assessment of the plan for pre-exposition vaccination with Fuenzalida-Palacios anti-rabies vaccine
Provedor de dados: 52
Autores: Preto,Angela Aparecida; Gomes,Elisa Maines; Fernandes,Maurício José; Hennig,Nei Adolfo; Germano,Pedro Manoel Leal.
Studies ere carried out to monitor the titres of anti-rabies antibodies taken from individuals exposed to the risk of infection. Fifty-seven individuals aged between 20 and 40, were vaccinated with a minimum titre of 1.3 IU Fuenzalida-Palacios type anti-rabies vaccine. The vaccination plan consisted of the application of three doses at two day intervals and a booster applied 28 days after the final dose. The accompainment of the antibody titres was achieved by means of the seroneutralization test over a total period of 18 months. Through the monitoring carried out, it was found to be necessary to apply a booster vaccination to part of the group. Some of these individuals were revaccinated with Fuenzalida-Palacios type vaccine with a minimum titre of 1.3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Rabies vaccine; Antibodies; Neutralization; Fuenzalida-Palacios.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300002

Investigation on Newcastle Disease Virus (NDV), Infectious Bursal Disease Virus (IBDV) and Avian Poxvirus (APV) in magellanic penguins in Southern region of Brazil
Provedor de dados: 52
Autores: Nunes,Cristina Freitas; Fonseca,Fabiane; Leite,Alice Teixeira Meirelles; Silva Filho,Rodolfo Pinho da; Finger,Paula Fonseca; Castro,Clarissa Caetano; Fischer,Geferson; Vargas,Gilberto D'avila; Hübner,Silvia de Oliveira.
To investigate the exposure of the Newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and avian poxvirus (APV) in Magellanic penguins found on the beaches in Southern regions of Brazil, the frequency of serum antibodies was estimated in 89 samples taken during 2005 and 2006. All the penguins were negative for the presence of antibodies against NDV by hemagglutination inhibition test and to APV by indirect ELISA. The reactivity was similar to the positives controls using ELISA kit for the IBDV made in the chickens in 50 samples. This reactivity also was demonstrated in 42 samples using agar gel immunodiffusion. No clinical signs related to IBDV infection were observed. The results indicated the absence of infection by NDV and APV but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magellanic penguins; Antibodies; Newcastle disease virus; Infectious bursal disease virus; Avian poxvirus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400008

Seroprevalence of antibodies against group A rotavirus in cattle from a pioneer frontier in Brazilian Amazon
Provedor de dados: 62
Autores: Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio.
Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003

Placental transfer of Haemophilus influenzae type b antibodies in malnourished pregnant women
Provedor de dados: 55
Autores: Cavalcante,Rejane S.; Kopelman,Benjamin I.; Costa-Carvalho,Beatriz T.
This study evaluated the vaccination response to Haemophilus influenzae type b (Hib) in malnourished pregnant women (MN), cord blood (CB) and in infants at two and six months of age for comparison with a control group (C). Twenty-eight malnourished pregnant women and 29 pregnant controls were immunized with conjugated Act-HIB® in the third trimester of pregnancy. Blood samples were collected from all before the immunization, during labor (post immunization), and from CB. All infants were immunized with Hib vaccine according to normal vaccine schedule and sera were collected at two and six months of age. Antibody levels to polyribosylribitol phosphate (PRP) were similar for both groups. Preimmunization: MN 1.94 µg/mL, C 1.68 µg/mL; post-vaccination: MN...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pregnant women; Malnourished; Haemophilus influenzae type b; Passive immunization; Vaccine; Antibodies; Immunoglobulin G.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100011

Are anti-interferon antibodies the cause of failure in: chronic HCV hepatitis treatment?
Provedor de dados: 55
Autores: Barone,Antonio Alci; Tosta,Rose Aparecida Borges; Tengan,Fátima Mitiko; Marins,José Humberto Caetano; Cavalheiro,Norma de Paula; Cardi,Bruno Andrade.
A follow-up study was made of 94 chronic hepatitis C patients at a hepatitis clinic in Brazil, after interferon alpha (IFN-alpha) therapy, to determine the influence of anti-interferon antibodies on treatment outcome. Patients diagnosed as having chronic hepatitis C, confirmed by PCR (HCV RNA) and liver biopsy, were treated with interferon alpha 2a or 2b for at least six months, and were followed up for 24 weeks after termination of treatment in order to assess biochemical, virological and clinical pathology responses. Only 6% of the 94 patients developed anti-IFN antibodies, 70% presented a biochemical response and 23% maintained a sustained virological response. Clinical evaluation revealed that in only 2 patients was there progression of fibrosis; the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon-alpha; Antibodies; Chronic HCV; Treatment.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100002

Epitope of dengue virus E protein detect human antibodies associated with mild disease: a potential peptide for vaccine development
Provedor de dados: 55
Autores: Sánchez-Burgos,Gilma; Herrera-Nájera,Carla; Cedillo-Rivera,Roberto; Dumonteil,Eric.
ABSTRACT The antigenic potential of seven immunogenic peptides of the dengue virus was evaluated in the sera of patients with dengue confirmed by IgM/IgG serology. Antibodies IgM and IgG against dengue virus peptides were analyzed by ELISA in 31 dengue sero-positive and 20 sero-negative patients. The P5 peptide showed significant IgG immunoreactivity mostly in the sera of patients with dengue without warning signs in comparison with patients with dengue with warning signs, correlating with mild disease. This finding suggests that the low antibody response against P5 epitope could be a risk factor for higher susceptibility to dengue virus infection with warning signs, and that P5 could be a potential antigen for vaccine development.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dengue virus; Epitopes; Peptides; Antibodies.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100085

Comparison of dot-ELISA and standard ELISA for detection of Neisseria meningitidis outer membrane complex-specific antibodies
Provedor de dados: 55
Autores: Belo,Elza FT; Farhat,Calil K; Gaspari,Elizabeth N De.
Dot-ELISA using the outer membrane complex antigens of Neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. We investigated the level of meningococcal-specific IgG, IgA, and IgM in serum using dot-ELISA with outer membrane antigens prepared from Neisseria meningitidis serotype B:4.19:P1.15,3,7,9 (a strain isolated from a Brazilian epidemic). The dot-ELISA is based on the same principles as the standard ELISA and is useful for detection of anti-N. meningitidis B antibodies in serum of patients with meningococcal infections. For the assay, outer membrane complexes (OMCs) were absorbed by nitrocellulose membrane and blocked with a 5% skim milk solution. Serum samples were drawn upon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria meningitidis; Dot-ELISA; ELISA; Antibodies; Outer membrane complex.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100008

Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay in patients from Northern Paraná State, Brazil
Provedor de dados: 55
Autores: Yoneyama,Kelly Aparecida Geraldo; Peder,Leyde Daiane de; Lonardoni,Maria Valdrinez Campana; Silveira,Thaís Gomes Verzignassi.
American cutaneous leishmaniasis (ACL) is an endemic disease caused by Leishmania parasites. The ACL diagnosis is commonly accomplished by parasitological and immunological methods. The objective of this work was to evaluate the enzyme immunoassay (EIA) for ACL laboratorial diagnosis. IgG antibodies against Leishmania (Viannia) braziliensis promastigotes were researched. For the method standardization 240 sera were used: 72 from patients with positive parasitological diagnosis, 38 from normal individuals and 113 from individuals with other pathologies. The sensibility was 93% and the specificity was 70%. Regarding diagnosis, EIA showed 97% positivity in patients with ACL and 97% negativity in patients with other cutaneous lesions. The EIA presented a good...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Diagnosis; Antibodies; Immunoenzyme; Leishmania braziliensis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300012

Prevalence of antibodies to Borrelia burgdorferi sensu stricto in humans from a Cuban village
Provedor de dados: 55
Autores: Rodríguez,Islay; Fernández,Carmen; Sánchez,Lizet; Martínez,Bárbara; Siegrist,Hans H.; Lienhard,Reto.
Lyme disease has not been officially reported in Cuba. However, clinical cases have been serologically reported. Seroprevalence survey of Borrelia burgdorferi sensu stricto antibodies in humans in the country has not been conducted. OBJECTIVE: To estimate the prevalence of borrelial antibodies in inhabitants of a village with historically high level of tick infestation. METHODS: Serum specimens from 247 persons randomly selected from the population of the village were examined by IgG Western blot using B31 strain for estimating the prevalence of antibodies profile. RESULTS: A seroprevalence value interval (95% CI) of 0.6%-7.2% was estimated for the studied population. The prevalent borrelial protein bands on immunoblots were 41, 72, 90/93, 34, 47, 60, 58,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Borrelia burgdorferi; Prevalence; Lyme disease; Antibodies.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000100015

Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in elderly residents of a long-term care facility
Provedor de dados: 55
Autores: Valenzuela B.,M. Teresa; Altuzarra H.,Rogelio; Trucco A.,Olivia; Villegas R.,Rodrigo; Inostroza S.,Jaime; Granata S.,Paulo; Fleiderman V.,José; Maggi C.,Leonardo.
S. pneumoniae is a significant cause of community-acquired pneumonia in the elderly, and accounts for the majority of the pneumonia deaths among the elderly. We conducted this randomized double-blind study to evaluate the immune response to a 23-valent pneumococcal polysaccharide vaccine and the persistence of antibodies two years after the vaccination in an elderly population in Santiago, Chile. A total of 118 elderly nursing home residents received either the pneumococcal or a tetanus control vaccine. Serum samples were taken at enrolment, at two months, and at two years post-vaccination. Pre-vaccination anti-pneumococcal antibody geometric mean concentrations (GMC) were similar in both study groups, with increased levels of antibodies found only against...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Elderly; Immunogenicity; Antibodies.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300005

Health care workers humoral immune response against GLcB, MPT51 and HSPX from Mycobacterium tuberculosis
Provedor de dados: 55
Autores: Reis,Michelle Cristina Guerreiro dos; Rabahi,Marcelo Fouad; Kipnis,André; Junqueira-Kipnis,Ana Paula.
Tuberculosis (TB) is one of the oldest human infectious diseases and one third of the world's population is latently infected. Brazil is an endemic area for TB. One of the most important challenges in TB control is the identification of latently infected individuals. Health Care Workers (HCW) are at high risk of being infected with Mycobacterium tuberculosis and even to become TB latently infected. The aim of this study was to increase knowledge about humoral immune response in TB latently infected individuals. HCW were classified according to their tuberculin skin test (TST), as positive or negative. The antibody response to GLcB, MPT51 and HSPX from Mycobacterium tuberculosis was evaluated. TST negative HCW constituted the majority of those who showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Latent tuberculosis; Infection; Antibodies.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000600006

Estimated prevalence of immunity to poliomyelitis in the city of São Paulo, Brazil: a population-based survey
Provedor de dados: 55
Autores: Kiffer,Carlos Roberto Veiga; Conceição,Orlando Jorge; Santos,Edgar Bortholi; Sabino,Ester; Focaccia,Roberto.
OBJECTIVES: Estimate the prevalence of immunity to poliomyelitis (anti-polio antibodies) in the city of São Paulo/Brazil through a population-based survey. METHODS: A quantitative and inductive method was used to draw a representative sample of the population. Randomization and stratification (based on sex, age and residence region) was done, and 1,059 individuals were studied on a home-visit basis (structured questionnaires and blood samples). A microneutralization test was performed to detect anti-polio antibodies against serotypes 1, 2 and 3. RESULTS: The estimated prevalence of immunity to poliomyelitis was high, with 94.6% prevalence of anti-polio 1 antibodies, 98.8% anti-polio 2 and 91.9% anti-polio 3. Despite this high prevalence, there were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunity prevalence; Poliomyelitis; Poliviruses; Seroepidemiologic studies; Antibodies; Viral blood.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000500004

Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages
Provedor de dados: 56
Autores: dos Santos,D.P.; Muniz,I.P.R.; Queiroz,A.F.; Pereira,I.S.; Souza,M.P.A.; Lima,L.J.; Sousa,L.R.O.; Ribeiro,I.S.; Galantini,M.P.L.; Marques,L.M.; Figueiredo,T.B.; da Silva,R.A.A.
Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Staphylococcus aureus; Mice; Air pouch; Antibodies.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500616

Evidence for the presence of a kininogen-like species in a case of total deficiency of low and high molecular weight kininogens
Provedor de dados: 56
Autores: Veloso,D.
Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178 &lt;FONT FACE="Symbol"&gt;®&lt;/FONT&gt; stop) has been blamed for the absence of LK and HK. However, using Western blots...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kininogen deficiency; Kinins; Antibodies; Plasma; Kininogen-like species; Human and nonhuman primates.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700004

HIV-1 binding and neutralizing antibodies of injecting drug users
Provedor de dados: 56
Autores: Ouverney,E.P.; Teixeira,S.L.M.; Silva-de-Jesus,C.; Hacker,M.A.; Morgado,M.G.; Bastos,F.I.; Bongertz,V.
Previous studies have demonstrated a stronger seroreactivity against some synthetic peptides responsible for inducing neutralizing antibodies in injecting drug users (IDU) compared to that of individuals sexually infected with HIV-1 (S), but the effectiveness in terms of the neutralizing ability of these antibodies has not been evaluated. Our objective was to study the humoral immune response of IDU by determining the specificity of their antibodies and the presence of neutralizing antibodies. The neutralization capacity against the HIV-1 isolate MN (genotype B), the primary HIV-1 isolate 95BRRJ021 (genotype F), and the seroreactivity with peptides known to induce neutralizing antibodies, from the V2 and V3 loops of different HIV-1 subtypes, were analyzed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Injecting drug users; Antibodies; Neutralization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900004

Anti-leishmania antibodies in cerebrospinal fluid from dogs with visceral leishmaniasis
Provedor de dados: 56
Autores: Lima,V.M.F.; Gonçalves,M.E.; Ikeda,F.A.; Luvizotto,M.C.R.; Feitosa,M.M.
Visceral leishmaniasis in Brazil is caused by Leishmania (Leishmania) chagasi and the dog is its most important reservoir. The clinical features in dogs include loss of weight, lymphadenopathy, renal failure, skin lesions, fever, hypergammaglobulinemia, hepatosplenomegaly, anemia, and, rarely, neurological symptoms. Most infected animals develop active disease, characterized by high anti-leishmania antibody titers and depressed lymphoproliferative ability. Antibody production is not primarily important for protection but might be involved in the pathogenesis of tissue lesions. An ELISA test was used to determine if there is an association between neurological symptoms and the presence of anti-L. chagasi antibodies in cerebrospinal fluid (CSF). Thirty serum...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Cerebrospinal fluid; Dogs; Antibodies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400010

DNA vaccines for viral diseases
Provedor de dados: 56
Autores: Donnelly,J.J.; Ulmer,J.B.
DNA plasmids encoding foreign proteins may be used as immunogens by direct intramuscular injection alone, or with various adjuvants and excipients, or by delivery of DNA-coated gold particles to the epidermis through biolistic immunization. Antibody, helper T lymphocyte, and cytotoxic T lymphocyte (CTL) responses have been induced in laboratory and domesticated animals by these methods. In a number of animal models, immune responses induced by DNA vaccination have been shown to be protective against challenge with various infectious agents. Immunization by injection of plasmids encoding foreign proteins has been used successfully as a research tool. This review summarizes the types of DNA vaccine vectors in common use, the immune responses and protective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasmid; Nucleic acid; Protection; Antibodies; T cells; CTL.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200010

Hormone replacement therapy increases levels of antibodies against heat shock protein 65 and certain species of oxidized low density lipoprotein
Provedor de dados: 56
Autores: Uint,L.; Gebara,O.C.E.; Pinto,L.B.; Wajngarten,M.; Boschcov,P.; Luz,P.L. da; Gidlund,M.
Hormone replacement therapy (HRT) reduces cardiovascular risks, although the initiation of therapy may be associated with transient adverse ischemic and thrombotic events. Antibodies against heat shock protein (Hsp) and oxidized low density lipoprotein (LDL) have been found in atherosclerotic lesions and plasma of patients with coronary artery disease and may play an important role in the pathogenesis of atherosclerosis. The aim of the present study was to assess the effects of HRT on the immune response by measuring plasma levels of antibodies against Hsp 65 and LDL with a low and high degree of copper-mediated oxidative modification of 20 postmenopausal women before and 90 days after receiving orally 0.625 mg equine conjugate estrogen plus 2.5 mg...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hormone replacement therapy; Postmenopause; Oxidized LDL; Heat shock protein; Immune response; Antibodies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400011

Sera from chronic chagasic patients depress cardiac electrogenesis and conduction
Provedor de dados: 56
Autores: Costa,P.C.S.; Fortes,F.S.A.; Machado,A.B.; Almeida,N.A.C.; Olivares,E.L.; Cabral,P.R.; Pedrosa,R.C.; Goldenberg,R.C.; Campos-de-Carvalho,A.C.; Masuda,M.O.
We report results obtained with sera from 58 chronic chagasic patients that were evaluated for effects on heart rate and atrioventricular (AV) conduction in isolated rabbit hearts and screened for the presence of muscarinic and beta-adrenergic activity. We show that sera from 26 patients decreased heart rate, while 10 increased it and 22 had no effect. Additionally, sera from 20 of the 58 patients blocked AV conduction. Muscarinic activation seems to be involved in both effects, but is not the only mechanism, since atropine did not antagonize the decrease in heart rate in 23% of sera or AV block in 40%. Sera from patients with complex arrhythmias were significantly more effective in depressing both heart rate and AV conduction. Sera that induce increases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas' disease; Antibodies; Muscarinic activation; Beta-adrenergic activation; Gap junction; Mammalian heart.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400010

Evaluation of antibodies production against Borrelia burgdorferi in cattle submitted to rBm86 protein Boophilus microplus tick immunization and associated challenges influence
Provedor de dados: 58
Autores: Ishikawa,Márcia Mayumi; Fonseca,Adivaldo Henrique; Yoshinari,Natalino Hajime; Osório,Ana Luiza Alves Rosa.
IgG antibodies production against Borrelia burgdorferi in immunized cattle with rBm86 protein from Boophilus microplus was evaluated as well as the influence of the association between immunizations and stress through indirect ELISA test during one year. In the present study there was no influence of the isolated challenged used on the production of IgG antibodies against B. burgdorferi. The rBm86 immunogen did not cause significant oscillation in the production of IgG antibodies against B. burgdorferi capable to interfere in the serological results for Lyme Borreliosis in cattle. This study demonstrated the possibility of transitory changes in the production of antibodies after the association of vaccine stimuli and stress, emphasizing the necessity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Borrelia; Cattle; Tick.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500032

Anti-influenza A virus antibodies in Tayassuidae from commercial rearing farms in Brazil
Provedor de dados: 65
Autores: Baraldi,Thaís Gasparini; Almeida,Henrique Meiroz de Souza; Morais,Amanda Bonalume Cordeiro de; Storino,Gabriel Yuri; Montassier,Hélio José; Motta,Rodrigo Garcia; Ribeiro,Márcio Garcia; Oliveira,Luís Guilherme de.
ABSTRACT:  Family Tayassuidae in the suborder Suina include two species of peccaries in Brazil: the white-lipped peccary (Tayassu pecari) and the collared peccary (Pecari tajacu). These animals share common pathogens with domestic swine (Sus scrofa); however, their role as potential carrier remains unclear. This study focused on detecting the prevalence of influenza A antibodies in Tayassu pecari and Pecari tajacu from commercial rearing farms from two states in Brazil. A set of 50 blood samples from Pecari tajacu and 55 from Tayassu pecari were analyzed using a commercial indirect ELISA in order to investigate anti influenza A antibodies. Pecari tajacu samples presented 22% (11/50) of seropositivity for the virus. Serological surveillance is an important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Influenza; Antibodies; Peccaries.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400451

A STUDY OF ASSOCIATION BETWEEN ELISA RESPONSE TO INFECTIOUS DISEASE AGENTS AND CALVING INTERVAL IN CATTLE IN THE TROPICS OF MÉXICO
Provedor de dados: 65
Autores: Barajas-Rojas,José Alfonso; Riemann,Hans; Franti,Charles.
Forty eight cows of Holstein-Zebu crosses with at least one completed pregnancy were tested for antibodies to 20 disease agents. The testing was performed within one month (average two weeks) after calving when the IgG level constantly reaches its lowest value during the cows reproductive cycle. The results indicate that increasing levels of IgG against Leptospira interrogans serovar hardjo and Mycobacterium paratuberculosis may be associated with an increased calving interval. Increase in calving intervals could not be explained by age of cows or their genotypic characteristics.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious diseases; Antibodies; Calving intervals; México.
Ano: 1993
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000300015