Consulta: subjectFacets:"Biodistribution"
Registros recuperados: 24
Data/hora: 13/06/2024 02:13:03

Technetium-99m-labeled deoxynivalenol from Fusarium mycotoxin alters organ toxicity in BALB/c mice by oral and intravenous route
Provedor de dados: 80
Autores: Chattopadhyay,P; Pandey,A; Goyary,D; Chaurasia,A; Singh,L; Veer,V.
The toxicity of deoxynivalenol, both intravenously and orally, was investigated in male and female BALB/c mice. Technetium-99m (99m Tc)-labeled deoxynivalenol was administered to mice by tail vein injection and orally dosed. Distribution of labeled deoxynivalenol at 26 hours was monitored by gamma-scintigraphy. In the evaluated organs, the accumulation of radioactive deoxynivalenol was correlated with the amount of radioactivity. In addition, the toxicity of deoxynivalenol was measured by biochemical assays followed by histopathological findings. Kidney and hepatic marker enzymes were significantly increased in intravenously administered deoxynivalenol as compared to orally treated mice. Intravenously treated mice showed severe damage in liver and kidney...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m (99mTc); Deoxynivalenol; Biodistribution; Toxicity; Histopathology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300002

Pharmacokinetics of neutron-irradiated meglumine antimoniate in Leishmania amazonensis-infected BALB/c mice
Provedor de dados: 80
Autores: Borborema,Samanta Etel Treiger; Osso Junior,João Alberto; Andrade Junior,Heitor Franco de; Nascimento,Nanci do.
Abstract  Background:  Cutaneous leishmaniasis (CL) is a parasitic disease caused by the protozoan Leishmania spp. Pentavalent antimonial agents have been used as an effective therapy, despite their side effects and resistant cases. Their pharmacokinetics remain largely unexplored. This study aimed to investigate the pharmacokinetic profile of meglumine antimoniate in a murine model of cutaneous leishmaniasis using a radiotracer approach.  Methods:  Meglumine antimoniate was neutron-irradiated inside a nuclear reactor and was administered once intraperitoneally to uninfected and L. amazonensis-infected BALB/c mice. Different organs and tissues were collected and the total antimony was measured.  Results:  Higher antimony levels were found in infected than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Meglumine antimoniate; Pharmacokinetics; Biodistribution; Antimony; Radioisotope.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100304

Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom
Provedor de dados: 80
Autores: Shirmardi,SP; Shamsaei,M; Gandomkar,M; Saniei,E; Ghannadi,M; Zare,A.
Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purification, labeling and biological evaluation of M. eupeus venom are described. For separation, soluble venom was loaded on a chromatography column packed with Sephadex G-50 gel. Subsequently, the fractions were collected according to UV absorption at a wavelength of 280 nm. Toxic fraction (F3) was loaded on an anionic ion exchanger resin and then on a cationic resin....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesobuthus eupeus; Venom; Purification; Chromatography; Radiolabeling; Biodistribution.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400015

Biodistribution studies of bee venom and spider toxin using radiotracers
Provedor de dados: 80
Autores: Yonamine,C. M.; Costa,H.; Silva,J. A. A.; Muramoto,E.; Rogero,J. R.; Troncone,L. R. P.; Camillo,M. A. P.
The use of radiotracers allows the understanding of the bioavailability process, biodistribution, and kinetics of any molecule labelled with an isotope, which does not alter the molecule's biological properties. In this work, technetium-99m and iodine-125 were chosen as radiotracers for biodistribution studies in mice using bee (Apis mellifera) venom and a toxin (PnTX2-6) from the Brazilian "armed" spider (Phoneutria nigriventer) venom. Incorporated radioactivity was measured in the blood, brain, heart, lung, liver, kidney, adrenal gland, spleen, stomach, testicle, intestine, muscle, and thyroid gland. Results provided the blood kinetic parameter, and different organs distribution rates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phoneutria nigriventer; Apis mellifera; Toxin; Venom; Radiotracer; Biodistribution.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100006

Biodistribution of the radiopharmaceutical technetium-99m-sodium phytate in rats after splenectomy
Provedor de dados: 52
Autores: Pereira,Kércia Regina Santos Gomes; Açucena,Maria Kadja Meneses Torres; Villarim Neto,Arthur; Rêgo,Amália Cínthia Meneses; Bernardo-Filho,Mário; Azevedo,Ítalo Medeiros; Araújo Filho,Irami; Medeiros,Aldo Cunha.
Drugs and surgery can interfere with the biodistribution of radiopharmaceuticals and data about the effect of splenectomy on the metabolism of phytate-Tc-99m are scarce. This study aimed at evaluating the interference of splenectomy on phytate-Tc-99m biodistribution and liver function in rats. The SP group rats (n=6) underwent splenectomy. In group C (control) the animals were not operated on. After 15 days, all rats were injected with 0.1mL of Tc-99m-phytate via orbital plexus (0.66MBq). After 30 minutes, liver samples were harvested, weighed and the percentage of radioactivity per gram (%ATI/g) was determined by a Wizard Perkin-Elme gama counter. The ATI%/g in splenectomized rats (0.99±0.02) was significantly higher than in controls (0.4±0.02),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Splenectomy; Sodium phytate; Biodistribution; Liver.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700033

Ethanol affects the absorption and tissue distribution of orally administered antigens in mice
Provedor de dados: 52
Autores: Oliveira,Flávia Márcia; Gusmão,Leandro José; Cardoso,Valbert do Nascimento; Ramaldes,Gilson Andrade; Ruiz-de-Souza,Valéria; Gontijo,Cristiano Machado.
The aim of this work was to evaluate the effects of ethanol on the adsorption and tissue distribution of orally administered antigens in mice. Results showed that ethanol reduced the level of anti-ovalbumin IgA antibodies in intestinal fluid for the mice treated orally with a palmitoyl-ovalbumin conjugate. Ethanol was administered intragastrically to mice at 5 g/kg body weight for 14 days (chronic treatment), or 10 g/kg body weight every 7th day up to 14 days (acute treatment). Thereafter, 99m technetium-labeled antigens were administered and lymphoid tissues were collected. Ethanol interfered with the transport of ovalbumin to the liver. Moreover, the transport of palmitoyl-ovalbumin to mesenteric lymph nodes was reduced 6 h after the antigen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Absorption; Biodistribution; Technetium; Immune response.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400008

Biodistribution of the radiopharmaceutical sodium pertechnetate after biliopancreatic bypass with a duodenal switch
Provedor de dados: 52
Autores: Araújo-Filho,Irami; Rêgo,Amália Cínthia Meneses; Brandão-Neto,José; Villarim-Neto,Arthur; Egito,Eryvaldo Sócrates Tabosa; Azevedo,Ítalo Medeiros; Medeiros,Aldo Cunha.
Study with the purpose to examine the effects of duodenal switch (DS), regularly performed in morbidly obese patients, on biodistribution of sodium pertechnetate in several organs of rats. There was no early or late mortality in either rats groups. The values of percent radioactivity per gram of tissue (%ATI/g), showed no significant difference in liver, stomach, small bowel, duodenum, kidney, heart, bladder, bone and brain, when compared the DS rats with sham and controls rats. A postoperative significant increase (p&lt;0.05) in mean %ATI/g levels was observed in spleen, pancreas and muscle in group DS rats, as compared to group S and C rats. In the lung there was an increase and in thyroid a decrease in mean %ATI/g of DS rats, when compared to sham rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bariatric surgery; Duodenal switch; Sodium pertechnetate; Biodistribution.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600024

Effect of tripanosomicide benznidazole (Rochagan®) on the biodistribution of sodium pertechnetate (Na99mTcO4) in Wistar rats
Provedor de dados: 52
Autores: Barbosa,Vanessa Santos de Arruda; Holanda,Maria de Carvalho Xavier; Silva,Roseane Pereira da; Oliveira,Daniel Pereira de; Silva Júnior,Maurício Ferreira da; Oliveira,Elias Herculano de; Spyrides,Maria Helena Constantino; Medeiros,Aldo Cunha.
Benznidazole, a drug with specific anti-Trypanosoma cruzi activity, is used in the treatment of Chagas' disease. The radiopharmaceutical sodium pertechnetate (Na99mTcO4) is used to obtain diagnostic images of the stomach, thyroid, parathyroids, salivary glands, brain and in the study of esophageal reflux and blood flow. This study aimed at evaluating in vivo the influence of benznidazole treatment on the sodium pertechnetate biodistribution in Wistar rats. The percentage of radioactivity per gram (%ATI/g) of various organs (brain, heart, esophagus, stomach, small intestine, large intestine, spleen, liver, muscle and blood) was determined. Comparing the treated rats with the controls, we observed that sodium pertechnetate biodistribution did not change when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiparasite; Radiopharmaceutical; Technetium-99m; Benznidazole; Biodistribution; Trypanosoma cruzi.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700028

Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents
Provedor de dados: 52
Autores: Diniz,Claudia Leite; Carmo,Fernanda Santos do; Almeida,Danielle Santos de; Santos-Filho,Sebastião David; Missailidis,Sotiris; Fonseca,Adenilson de Souza da; Bernardo Filho,Mario.
This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p&lt;0.05) the %ATI/g in several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodistribution; Centella asiatica; Blood; Plasma; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700035

Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats
Provedor de dados: 52
Autores: Açucena,Maria Kadja Meneses Torres; Pereira,Kércia Regina Santos Gomes; Villarim Neto,Arthur; Rêgo,Amália Cínthia Meneses; Bernardo-Filho,Mario; Azevedo,Ítalo Medeiros; Araújo Filho,Irami; Medeiros,Aldo Cunha.
This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p&lt;0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p&lt;0.05) of splenectomized animals, a significant increase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Splenectomy; Biodistribution; Technetium; Rats.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700032

Preparation and evaluation of modified composition for lyophilized kits of [Cu(MIBI)4]BF4 for [99mTc] technetium labeling
Provedor de dados: 52
Autores: Lima,Marisa de Jesus de C.; Marques,Fabio Luiz Navarro; Okamoto,Miriam Roseli Yoshie; Garcez,Alexandre Tales; Sapienza,Marcelo Tatit; Buchpiguel,Carlos Alberto.
The [Cu(MIBI)4]BF4 complex was synthesized and different formulations for lyophilized kits that could be cost-effectively used with different routines in nuclear medicine laboratories were investigated. In one preparation the kit components were kept similar to the Cardiolite® , except that the SnCl2.2H2O concentration was increased to 0.150 mg. In a second formulation, component concentrations were reduced to 1/5 of the original value and the SnCl2.2H2O concentration was adjusted to 0.04 mg. These products were labeled with maximum activities of 55.5 GBq and 8.14 GBq, respectively, and have shown an average radiochemical purity of 95 %. Biodistribution of the products was assessed by dissection in mice and in rabbits, and did not show any statistical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: [99mTc]technetium; [[99mTc](MIBI)6]+; Radiopharmaceutical; Biodistribution.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700001

Use of labelled cisplatin obtained by direct irradiation in a preliminary biodistribution study
Provedor de dados: 52
Autores: Carvalho Júnior,Álvaro Dutra de; Abrantes,Fabiana Moreira; Menezes,Maria Ângela; Leal,Alexandre Soares; Cardoso,Valbert Nascimento; Oliveira,Mônica Cristina de.
This work presents the preparation of radiolabelled cis-dichlorodiammineplatinum (II), CDDP*, from its direct irradiation into a cadmium capsule, using the TRIGA MARK I IPR R-1 research reactor of the CDTN. The ability to detect CDDP* in Ehrlich tumour-bearing mice after administration via an intravenous route was evaluated. After 24 hours, blood and some organs were collected to determine the incorporated activity. The CDDP* showed a great chemical purity and high specific activity that resulted in an optimum in vivo detection. The CDDP* was taken up principally by liver, spleen and kidney. The CDDP* obtained from this condition was shown to be a good tool for biodistribution studies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiolabelled cisplatin; Biodistribution; TRIGA research reactor.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700013

Experimental models using radionuclides for assessment of the effects of natural drugs
Provedor de dados: 52
Autores: Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Rebello,Bernardo; Rocha,Emely Kazan; Timóteo,Margareth de Oliveira; Olej,Beni; Bernardo Filho,Mário; Caldas,Luiz Querino de Araújo.
The aim of this study was to evaluate the effect of the extracts of Nectandra membranacea (N. membranacea), Ginkgo biloba (EGb) and Passiflora (PEF) on the morphology of red blood cells (RBC), on the biodistribution of sodium pertechnetate (99mTcO4Na), on the morphology of duodenum and on the labeling of blood constituents (BC, IF-P, IF-BC) with technetium-99m (Tc-99m). Morphometry studies also were performed. The results show that EGb promotes alteration of the labeling of BC, IF-P and IF-BC (p&lt;0.05). The N. membranacea extract does not promote significant alteration of the radiolabeling, and PEF extract alters the IF-P labeling. N. membranacea, EGb and PEF extracts were able to alter the RBC morphology (P&lt;0.05). N. membranacea extract and EGb...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nectandra membranacea (N. membranacea); Ginkgo biloba (EGb); Passiflora edulis flavicarpa (PEF); Blood constituents (BC) labeling; Technetium-99m; Biodistribution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700027

Ultrastructural analysis of kidney, liver and duodenum isolated from treated rats with Ginkgo Biloba extract and effects of this medicinal plant on the biodistribution of the padiopharmaceutical sodium pertechnetate
Provedor de dados: 52
Autores: Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Nascimento,Ana Lúcia; Arnobio,Adriano; Olej,Beni; Timóteo,Margareth de Oliveira; Caldas,Luiz Querino de Araújo; Bernardo Filho,Mário.
Ginkgo biloba extract (EGb) has been used to treat memory and concentration deficits, acts as platelet activating factor antagonism and prevents against damages caused by free radicals. EGb is a standardized extract that contains 24% flavonoids and 6% terpenoids. The aim of this work was to evaluate the possible influence of an EGb on the ultrastructure of some organs isolated from rats and on the biodistribution of sodium pertechnetate (99mTcO4Na). The animals were treated with EGb and after six days, received 99mTcO4Na. The organs were isolated and fixed for ultrastructural analysis. The results showed that EGb has modified the ultrastructure of kidney, liver and duodenum and altered the biodistribution of 99mTcO4Na (P&lt;0.05). It is speculated that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginkgo biloba; Technetium-99m; Biodistribution; Morphology; Ultrastructure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700030

Effects of chronic sucralose sweetener on the labeling of blood constituents with technetium-99m, morphology of red blood cells and the biodistribution of sodium pertechnetate in rats
Provedor de dados: 52
Autores: Rocha,Gabrielle de Souza; Pereira,Marcia de Oliveira; Benarroz,Mônica de Oliveira; Frydman,Jacques Natan Grinapel; Garcia-Pinto,Angélica Beatriz; Pereira,Mário José; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodistribution; Blood; Morphology; Sweetener; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700021

Effect of medicinal plants on the parasitemia of Trypanosoma cruzi and on the biodistribution of sodium pertechnetate (Na99mTcO4)
Provedor de dados: 52
Autores: Silva,Roseane Pereira da; Holanda,Cecília Maria de Carvalho Xavier; Barbosa,Vanessa Santos de Arruda; Oliveira,Daniel Pereira de; Lima,Natália Alves; Câmara,Antônia Cláudia Jácome da; Medeiros,Aldo da Cunha; Constantino,Maria Helena Spyrides.
Artemisia vulgaris (AV) is an antihelmintic and antimalarial drug; Aloe vera(babosa) acts as antidiabetic, laxative and anti-inflammatory;Benznidazole (BZ) is a trypanocidal of Trypanosoma cruzi (TC). Technetium-99m (99mTc) has been used in nuclear medicine to obtain diagnostic images. This study evaluated the plant effects in TC parasitemia and on the biodistribution of 99mTc in mice. Twenty mice were infected by TC. At the peak of parasitemia, 5 mice received babosa; 5 received AV and 5 received BZ. The parasitemia was determined at 0, 2, 4 and 6 h of drugs administration. Five infected mice without drugs, 5 mice without TC and the group treated with AV, received 99mTc. The radioactivity was calculated. Infected mice that received babosa reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aloe vera; Artemisia vulgaris; Technetium-99m; Trypanosoma cruzi; Parasitemia; Biodistribution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700034

Importance of the nutritional status for the interpretation of nuclear medicine examinations
Provedor de dados: 52
Autores: Moura,Egberto Gaspar de; Passos,Magna Cottini Fonseca.
Malnutrition is very prevalent in the Third World, but still in developing Countries and is found in certain communities in developed Countries. Several laboratories examinations are affected by malnutrition. Recently, gestational or neonatal malnutrition were considered to contribute to the development of chronic diseases in adulthood, this phenomena was named programming or metabolic imprinting. Similar consideration were suggested for Nuclear Medicine examinations. Here we review the literature about this aspect and present our own data showing changes in biodistribution of a radiopharmaceutical compound in different animal models of adult malnutrition or caused by maternal malnutrition programming.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Programming; Nuclear Medicine; Malnutrition; Biodistribution; Radiopharmaceuticals.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500021

Plasma clearance and biodistribution of oxidatively modified 99mTc-ß-VLDL in rabbits
Provedor de dados: 56
The biodistribution and removal from plasma (measured as fractional clearance rate, FCR, per hour) of native and oxidatively modified 99mtechnetium-labeled ß-very low density lipoprotein (99mTc-ß-VLDL) were investigated in hypercholesterolemic (HC) and control (C) three-month old New Zealand rabbits. The intracellular accumulation of ß-VLDL labeled with 99mTc was studied in vitro in THP-1 cells and monocyte-derived macrophages isolated from rabbits. After intravenous injection into C rabbits, copper-oxidized ß-VLDL (99mTc-ox-ß-VLDL) was cleared from the circulation faster (0.362 ± 0.070/h) than native ß-VLDL (99mTc-nat-ß-VLDL, 0.241 ± 0.070/h). In contrast, the FCR of 99mTc-ox-ß-VLDL in HC rabbits was lower (0.100 ± 0.048/h) than that of 99mTc-nat-ß-VLDL...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SS-very low density lipoprotein; Copper-mediated oxidation; Biodistribution; Fractional clearance rate; Atherosclerosis; Hypercholesterolemia; Cholesterol feeding; Rabbit.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600002

Effect of oral ingestion of an extract of the herb Uncaria tomentosa on the biodistribution of sodium pertechnetate in rats
Provedor de dados: 56
Autores: Moreno,S.R.F.; Silva,A.L.C.; Diré,G.; Honeycut,H.; Carvalho,J.J.; Nascimento,A.L.; Pereira,M.; Rocha,E.K.; Oliveira-Timóteo,M.; Arnobio,A.; Olej,B.; Bernardo-Filho,M.; Caldas,L.Q.A.
The aim of the present study was to determine the effect of the oral ingestion of an extract of the herb Uncaria tomentosa (cat's claw) on the biodistribution of the radiobiocomplex sodium pertechnetate (Na99mTcO4) in rats. The animals (male Wistar rats, 2 months old, 180-220 g), were treated (1 mL) with an U. tomentosa extract (32 mg/mL, N = 5) or 0.9% NaCl solution (control, N = 5) for 7 days. After this period, Na99mTcO4 (3.7 MBq, 0.3 mL) was injected through the ocular plexus and after 10 min the rats were killed, the organs isolated and counted in a well-gamma counter. A significant (P &lt; 0.05) alteration in Na99mTcO4 uptake i) from 0.57 ± 0.008 to 0.39 ± 0.06 %ATI/organ (P &lt; 0.05) and from 0.57 ± 0.17 to 0.39 ± 0.14 %ATI/g (P &lt; 0.05) was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Uncaria tomentosa; Biodistribution; Sodium pertechnetate; Radiobiocomplex.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100010

Biodistribution of 99m technetium- labeled creatinine in healthy rats
Provedor de dados: 56
Autores: Yilmaz,O.; Yurt Lambrecht,F.; Soylu,A.; Durkan,K.; Kavukcu,S.
The distribution of creatinine, one of the toxic guanidine compounds, in various tissues has not been studied in detail by using radiolabeled creatinine. Our objective was to investigate the biodistribution of creatinine labeled with 99m technetium (99mTc) by the stannous (II) chloride method in healthy male Wistar rats. Quality controls were carried out by radio thin layer chromatography, high-performance liquid chromatography, and paper electrophoresis. The labeling yield was 85 ± 2% under optimum conditions (pH 7 and 100 µg stannous chloride). Rats (N = 12) were injected intravenously with 99mTc-creatinine and their blood and visceral organs were evaluated for 99mTc-creatinine uptake as percent of the injected dose per gram wet weight of each tissue...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biodistribution; Creatinine; 99mTechnetium; 99mTc-creatinine; Rat.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600009

Distribution of liposome-encapsulated antimony in dogs
Provedor de dados: 56
Autores: Schettini,D.A.; Costa Val,A.P.; Souza,L.F.; Demicheli,C.; Rocha,O.G.F.; Melo,M.N.; Michalick,M.S.M.; Frézard,F.
The achievement of complete cure in dogs with visceral leishmaniasis is currently a great challenge, since dogs are the main reservoir for the transmission of visceral leishmaniasis to humans and they respond poorly to conventional treatment with pentavalent antimonials. In order to improve the efficacy of treatment, we developed a novel formulation for meglumine antimoniate based on the encapsulation of this drug in freeze-dried liposomes (LMA). The aim of the present study was to evaluate the biodistribution of antimony (Sb) in dogs following a single intravenous bolus injection of LMA. Four healthy male mongrel dogs received LMA at 3.8 mg Sb/kg body weight and were sacrificed 3, 48 and 96 h and 7 days later. Antimony was determined in the blood, liver,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Liposomes; Meglumine antimoniate; Biodistribution; Leishmaniasis; Dogs.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200015

Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice
Provedor de dados: 56
Autores: Carlesso,F.N.; Fuscaldi,L.L.; Araújo,R.S.; Teixeira,C.S.; Oliveira,M.C.; Fernandes,S.O.A.; Cassali,G.D.; Reis,D.C.; Barros,A.L.B.; Cardoso,V.N.
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using99mTc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 99mTc-HYNIC-βAla-Bombesin; Capan-1; Scintigraphic images; Pancreas tumor; Biodistribution.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000010

Radiolabeling of cidofovir with technetium-99m and biodistribution studies
Provedor de dados: 60
Autores: Arribada,Raquel Gregorio; Pereira,Nara Caroline; Cardoso,Valbert Nascimento; Cunha Júnior,Armando da Silva; Barros,André Luís Branco de.
Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cidofovir; Radiotracer; Technetium-99m; Biodistribution; Scintigraphic imaging.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100570

Solid dispersion-based pellet for colon delivery of tacrolimus through time- and pH-dependent layer coating: preparation, in vitro and in vivo studies
Provedor de dados: 60
Autores: Guo,Jian; Fang,Huihui; Gui,Shuangying; Huang,Yuzhe.
The intent of the present investigation is to develop and evaluate colon-specific coated tacrolimus solid dispersion pellet (SDP) that retards drug release in the stomach and small intestine but progressively releases in the colon. Tacrolimus-SDP was prepared by extrusion-spheronization technology and optimized by the micromeritic properties including flowability, friability, yields and dissolution rate. Subsequently, the pH-dependent layer (Eudragit L30D55) and time-dependent layer (Eudragit NE30D and L30D55) were coated on the SDP to form tacrolimus colon-specific pellets (CSP) using a fluidized bed coater. Under in vitro gradient pH environment, tacrolimus only released from CSP after changing pH to 6.8 and then quickly released in the phosphate buffer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tacrolimus; Solid dispersion; Pellet; Oral colon-specific; Biodistribution.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100557