Consulta: subjectFacets:"Collagen"
Registros recuperados: 39
Data/hora: 14/06/2024 17:18:15

Methylation-specific PCR analysis in Col8A1 promoter in Creole cattle carrier of rob(1;29)
Provedor de dados: 69
Autores: Postiglioni,Alicia; García,Cristina B; Rincón,Gonzalo; Arruga,M. Victoria.
Robertsonian translocation (rob(1;29)) is the most frequent structural chromosomal abnormality in cattle. Heterozygous carriers have a normal phenotype but show a 3-5% decrease in fertility. Chromatin decondensation was evaluated similar to the inactive X chromosome when submitted to demethylating agent. Based on this result, and the concept that imprinted genes are essential in embryonic development, we decided to query genes located on BTA1 and BTA29 that could undergo genome imprinting. The collagen typeVIII-Î±1 (Col8A1) acted on extracellular matrix structural proteins. DNA bisulfite conversion and sequentiation methods were used to compare its differential methylation patterns. It was performed on eight Creole cattle DNA blood samples from normal and...
Tipo: Journal article
Palavras-chave: Bisulfite; Collagen; Creole cattle; Cytosines; Methylation.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300013

Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly
Provedor de dados: 5
Autores: Norris, Karl; Mishukova, Oksana; Zykwinska, Agata; Colliec-jouault, Sylvia; Sinquin, Corinne; Koptioug, Andrei; Cuenot, Stéphane; Kerns, Jemma; Surmeneva, Maria; Surmenev, Roman; Douglas, Timothy.
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of...
Tipo: Text
Palavras-chave: Marine exopolysaccharide; Collagen; Surface modification; Ti6Al4V.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00477/58881/61433.pdf

Unusual Glycosaminoglycans from a Deep Sea Hydrothermal Bacterium Improve Fibrillar Collagen Structuring and Fibroblast Activities in Engineered Connective Tissues
Provedor de dados: 5
Autores: Senni, Karim; Gueniche, Farida; Changotade, Sylvie; Septier, Dominique; Sinquin, Corinne; Ratiskol, Jacqueline; Lutomski, Didier; Godeau, Gaston; Guezennec, Jean; Colliec-jouault, Sylvia.
Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800...
Tipo: Text
Palavras-chave: Marine hydrothermal bacteria; Glycosaminoglycan-mimetic; Collagen; Matrix metalloproteinases; Dermal fibroblast; Polysaccharides; Tissue engineering.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00138/24973/23072.pdf

Contribution Towards the Anatomy of the Esophageal Hiatus and its Relationship with the Presence of Bundles of Collagen Fibers in its Margins
Provedor de dados: 77
Autores: Filho,José Jeová de Oliveira; Filho,Benedito Herani; Reis,Francisco Prado; Feitosa,Vera Lúcia Correa; Aragão,José Aderval.
The aim was to detect the presence of bundles of collagen fibers in the margins of the esophageal hiatus and establish if there is any relationship of these bundles with the anatomy of the hiatus. Ten adult male cadavers, with no gross anatomical alteration caused by trauma, surgery or disease, upon the esophageal hiatus were used in the study. A piece of anatomical structure comprising the diaphragm with the esophageal hiatus and adjacent tissues was removed, dissected and 10% formol embedding. With the aid of a digital caliper, measurements of the perimeter of the esophageal hiatus were done both in the abdominal and thoracic sides. For the structural study each margin was divided in six sections. Staining techniques of Masson and Picrosirius-hematoxilin...
Tipo: Journal article
Palavras-chave: Diaphragm; Esophagogastric Junction; Collagen; Cadaver; Humans.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300015

Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy
Provedor de dados: 77
Autores: Sanchis,E. G; Cristofolini,A. L; Taglialegna,A; Merkis,C. I.
The molecules that constitute the extracellular matrix are important in several functions related to tissue support and cell-cell, cell-extracellular matrix interaction. Among the macromolecules that constitute the mentioned matrix we find osteopontin, fibrinogen and collagen. The present study was undertaken to analyze the rol of osteopontin, fibrinogen and collagen in uterine-placental interface during normal porcine gestation. Uterine and placental tissues from crossbred gilts of 30 (n=5), 60 (n=5), 70 (n=5) and 114 (at term, n=5) days of gestation were used. Macroscopic analysis of the embryos/fetuses allowed us to determine their gestational age by means of the crown-rump lenght. Haematoxylin-Eosin and Masson's Trichrome dyes along with light...
Tipo: Journal article
Palavras-chave: Pig; Placenta; Osteopontin; Fibrinogen; Collagen.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400062

ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS
Provedor de dados: 77
Autores: Pinto de Medeiros,Juliana; Wanderley Teixeira,Valéria; Aguiar Coelho Teixeira,Álvaro; Baratella Evêncio,Liriane; Evêncio Neto,Joaquim.
Some studies have shown that pinealectomized female rats present a large concentration of collagen fibers in the endometrium. The lack of light results in morphologic alterations in the uterine lamina propria and in the muscular layer, reducing thus the uterine weight. Therefore, this work aimed to analyze ultra-structurally the influence of melatonin in the remodeling of the collagen in the endometrium, in the estrus phase, in pinealectomized adult rats or submitted to lack of light. The experimental groups: I- sham-pinealectomized maintained in a clear/dark cycle 12/12 hours, during three months; II- pinealectomized rats maintained in a clear/dark cycle 12/12 hours, for three months; III- normal rats maintained in clear/dark cycle 12/12 hours for three...
Tipo: Journal article
Palavras-chave: Melatonin; Endometrium; Pinealectomy; Collagen.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300008

Fractal Analysis of Skeletal Muscle Tissue of Rats Subjected to Stretch Injury
Provedor de dados: 77
Autores: Ozaki,Guilherme; Camargo,Regina Celi Trindade; Koike,Tatiana Emy; Garcia,Thiago Alves; Castoldi,Robson Chacon; Camargo Filho,Jose Carlos Silva.
The aim of this study was to analyze changes in the skeletal muscle tissue of rats after muscle stretch injury using fractal analysis. Nineteen rats were divided into three groups, Control (n= 5) (C), Lesion two days (n= 7) (L2), and Lesion seven days (n= 7) (L7). Histological slides of the gastrocnemius muscle of the animals were prepared and stained with Hematoxylin and eosin for analysis of the inflammatory process, and picrosirius for the demonstration of collagen. The stained slides were subsequently analyzed using Fractal Dimension (FD). The results showed high FD values of the inflammatory process in the experimental groups L2 and L7 in relation to C (p= 0.000). The analysis of collagen in the picrosirius stained slides showed high FD in the L2...
Tipo: Journal article
Palavras-chave: Fractal analysis; Skeletal muscle injury; Collagen; Inflammation; Pathology.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300017

Morphometric and Fractal Analysis of Injured Skeletal Muscle Tissue Subjected to A Combination of Treatments; Cryotherapy and Therapeutic Ultrasound
Provedor de dados: 77
Autores: Koike,Tatiana Emy; Camargo,Regina Celi Trindade; Ozaki,Guilherme Akio Tamura; Castoldi,Robson Chacon; Seraphim,Patrícia Monteiro; Oikawa,Sérgio Minoro; Camargo Filho,José Carlos Silva.
The aim of this study was to analyze the effects of an association of cryotherapy and therapeutic ultrasound on the treatment of muscle injured by impact. Fifty-five Wistar rats were divided into five groups (n = 11), Acute Injury (AI), Injury (I), Cryotherapy (CR), Therapeutic Ultrasound (TU) and Association of Cryotherapy and Therapeutic Ultrasound (CRTU). The CR and CRTU groups received applications of Cryotherapy three times (immediately, 24 and 48 h after injury) of 20 minutes duration. The TU and CRTU groups received applications of Therapeutic Ultrasound for seven days, for five minutes, in pulsed mode, 0.5 w/cm intensity, frequency 1 MHz. Body mass and gastrocnemius mass were analyzed. In addition to histological slides stained with hematoxylin and...
Tipo: Journal article
Palavras-chave: Fractal; Regeneration; Cryotherapy; Ultrasound Therapy; Collagen.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300042

Quantitative Analysis of Collagen Fibrillar Structure in Canine Uterus Exposed to Acetate of Medroxyprogesterone Using Second Harmonic Generation Microscopy
Provedor de dados: 77
Autores: Salinas,Paulo; Miglino,Maria Angélica; del Sol,Mariano.
Collagen is the most abundant structural protein found in organs and is responsible for providing tissues with structure and function. In order to investigate in canine uteri the potential effect of medroxyprogesterone acetate (MPA) on the changes in collagen deposition were grouped as nulliparous (n=11), multiparous (n=11) and treated with MPA (n=11; nulliparous with two treatments; 5 mg/kg; i.m.). The amount of collagen was studied in the fold and basal regions of the endometrium and myometrium using second harmonic generation with a two-photon spectral confocal microscope, quantified using ImageJ software with a color segmentation plugin, was expressed as fraction area (%) and analyzed by ANOVA (p&lt;0,05). No differences were observed between groups in...
Tipo: Journal article
Palavras-chave: Canine; Medroxyprogesterone acetate; Collagen; Second harmonic generation.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100025

Lean vs. Obese Mice: The Ventral Prostate Revisited
Provedor de dados: 77
Autores: Martins,Fabiane Ferreira; del Sol,Mariano; Aguila,Marcia Barbosa; Mandarim-de-Lacerda,Carlos Alberto.
Obese mice (C57BL/6J-ob/ob) do not express leptin and develops hyperphagia, decreased energy expenditure, obesity, hyperglycemia, hyperinsulinemia, hypothermia, and infertility. Obesity causes reproductive dysfunction with negative impacts on prostatic structure and fertility. We aimed to compare the structure and molecular aspects of the ventral prostate between of lean and obese (ob/ob) mice. Three months old male lean and obese mice had their prostates dissected and prepared for light microscopy and immunofluorescence. In comparison to the lean mouse, the obese mouse showed a substantial structural modification in the ventral prostate starting with an atrophy of the prostate ventral lobe. Histologically, the acini showed a reduction in size, and in the...
Tipo: Journal article
Palavras-chave: Collagen; IL6; TNFalpha; Alpha-actin; Reproduction; Ob/ob mouse.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200003

Sub-lethal injection of honeybee venom decreased the levels of endogenously present substances in organs of mice
Provedor de dados: 79
Autores: LIPPS,B. V.
Pharmacological substances such as adenosine deaminase (ADA), collagen, histamine, IgE, myoglobin, and nerve growth factor (NGF) are endogenously present in animals. Research from this laboratory reported decreased levels of ADA, histamine, IgE, and NGF in organs of mice injected with sub-lethal doses of cobra venom. The goal of this research is to observe the levels of ADA, collagen, histamine, IgE, myoglobin, and NGF in certain organs of mice injected with venom from the bee Apis mellifera. Adult Balb/c female mice IM injected with half lethal dose of bee venom were sacrificed after 2 and 8 hours for removal of organs. The homogenates of the organs were assayed by ELISA for ADA, collagen, histamine, IgE, myoglobin, and NGF using respective antisera....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Honeybee venom; Adenosine deaminase; Collagen; Histamine; IgE; Myoglobin; Nerve growth factor.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200006

Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua
Provedor de dados: 80
Autores: Sato,Ana Claudia; Bosch,Rosemary Viola; Will,Sonia Elisabete Alves; Alvarez-Flores,Miryam Paola; Goldfeder,Mauricio Barbugiani; Pasqualoto,Kerly Fernanda Mesquita; Silva,Bárbara Athayde Vaz Galvão da; Andrade,Sonia Aparecida de; Chudzinski-Tavassi,Ana Marisa.
Abstract  Background  Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar Lonomia obliqua, presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac’s in vivo effects using a skin wound healing model in rats.  Methods  Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolin; RLosac; Wound healing; Collagen.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100324

Detection of Collagen-cross Reactive Antigenic Components in Toxoplasma gondii
Provedor de dados: 19
Autores: Omata, Yoshitaka; Inoue, Noboru; Yonematsu, Kunio; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Collagen; Cross-reactive antigens; Toxoplasma gondii.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/223

Biaxial testing of canine annulus fibrosus tissue under changing salt concentrations
Provedor de dados: 42
Autores: Huyghe,Jacques M.
The in vivo mechanics of the annulus fibrosus of the intervertebral disc is one of biaxial rather than uniaxial loading. The material properties of the annulus are intimately linked to the osmolarity in the tissue. This paper presents biaxial relaxation experiments of canine annulus fibrosus tissue under stepwise changes of external salt concentration. The force tracings show that stresses are strongly dependent on time, salt concentration and orientation. The force tracing signature of are sponse to a change instrain, is one of a jumpin stress that relaxes partly as the new strain is maintained. The force tracing signature of a stepwise change in salt concentration is a progressive monotonous change in stress towards a new equilibrium value. Although the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swelling; Collagen; Osmosis; Donnan; Cartilaginous.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000100012

The extracellular matrix of the lung and its role in edema formation
Provedor de dados: 42
Autores: Pelosi,Paolo; Rocco,Patricia R.M.; Negrini,Daniela; Passi,Alberto.
The extracellular matrix is composed of a three-dimensional fiber mesh filled with different macromolecules such as: collagen (mainly type I and III), elastin, glycosaminoglycans, and proteoglycans. In the lung, the extracellular matrix has several functions which provide: 1) mechanical tensile and compressive strength and elasticity, 2) low mechanical tissue compliance contributing to the maintenance of normal interstitial fluid dynamics, 3) low resistive pathway for an effective gas exchange, d) control of cell behavior by the binding of growth factors, chemokines, cytokines and the interaction with cell-surface receptors, and e) tissue repair and remodeling. Fragmentation and disorganization of extracellular matrix components comprises the protective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen; Glycosaminoglycans; Proteoglycan; Interstitial pressure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000200010

Histological evaluation of the periodontal ligament from aged wistar rats supplemented with ascorbic acid
Provedor de dados: 42
Autores: Zanoni,Jacqueline N.; Lucas,Nathalia M.; Trevizan,Aline R.; Souza,Ivan D.S.
Ascorbic acid (AA) is able to neutralize reactive oxygen species and is essential for collagen synthesis. In aging process oxidative stress is elevated. This study aims to investigate the effects of AA supplementation on the periodontal ligament (PL) of rats during aging. Twenty five rats were used and divided into groups: J90 (90-day-old control), E345 (345-day-old control), E428 (428-day-old control), EA345 (345-day-old supplemented with AA from 90-day-old on) and EA428 (428-day-old supplemented with AA from 90-day-old on). We analyzed the thickness, density of fibroblasts and blood vessels and collagen fibers types in the PL. In group J90 there was predominantly type III collagen fibers (87.64%). In animals supplemented with AA, the area filled by type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Ascorbic acid; Collagen; Periodontal ligament.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100327

Concentration of collagen fibers in the musculature of broiler chickens fed with cupuacu seed by-product
Provedor de dados: 45
Autores: Silveira,T.B.; Souza,S.F.; Medeiros,L.S.; Freitas,H.J.; Gracci,R.E.; Miglino,M.A.; Carvalho,Y.K.
ABSTRACT In this essay, we quantify the concentration of collagen fibers in broiler chickens exposed to increasing concentrations of cupuacu seed by-product. Collection of material was carried out in five chickens per treatment at 70 days old in the groups: control, 5% and 10% inclusion of cupuacu seed by-product. Fragments of Thoracic Pectoralis (PT) and Iliotibial lateralis (ITL) muscles were prepared for light and electronic microscopy. The amount of collagen fibers in the muscle groups was 1.08±0.61% in the PTC group; 6.24±2.58% in PT5% and 7.30±2.75% in PT10%. In the Iliotibial Lateralis groups, the results were 6.96±3.14% in the ITLC; 7.43±4.22% in the ITL5% and 8.66±2.35% in ITL10%. The amount of collagen fibers in the ITL5% and ITL10% groups showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler chickens; Collagen; Theobroma grandiflorum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300791

Placental osseous metaplasia associated with abortion in a mare: case report
Provedor de dados: 45
Autores: Lima,P.A.; Eckstein,C.; Ecco,R.; Santos,R.L.
ABSTRACT The aim of this report is to describe a case of marked osseous metaplasia in the placenta associated with abortion in a mare. A 19-year-old Campolina mare with a history of multiple parturitions and absence of reproductive failure, aborted a fetus with approximately six months of gestation. There were no gross changes in the fetus and the placenta. Microscopically, there was multifocal replacement of fetal connective mesenchyme of the chorionic villi with well differentiated and partially mineralized osseous tissue (confirmed by von Kossa staining). To further characterize the collagenous component of the lesion, sections of the placenta were stained with Masson’s trichrome and Picrosirius red. Osseous tissue deposition had abundance of type I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Ossification; Osseous matrix; Collagen; Chorionic villi.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100053

Dermatosparaxis in two White Doper lambs in Brazil: case report
Provedor de dados: 45
Autores: Silva,A.P.C.; Mol,J.P.S.; Carvalho Junior,C.A.; Paixão,T.A.; Santos,R.L.
Dermatosparaxis is a genetic disease that affects collagen maturation. This disease is characterized by marked impairment of the resistance of collagen fibers that leads to skin fragility, and it may affect several species. This is the first report of dermatosparaxis in sheep in Brazil. Clinically, two White Dorper lambs had multiple skin lacerations in the neck, dorsum and limbs. Transmission microscopy demonstrated irregular collagen fibers arranged in hieroglyphic shape and scanning electron microscopy demonstrated thin collagen fibrils that were not arranged in bundles as observed in the normal dermis. These findings are consistent with the diagnosis of dermatosparaxis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Dermatosparaxis; Collagen; Skin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300741

Extracellular matrix of ostrich articular cartilage
Provedor de dados: 47
Autores: Tomiosso,Tatiana Carla; Gomes,Laurecir; de Campos Vidal,Benedicto; Pimentel,Edson Rosa.
The composition and organization of the extracellular matrix of ostrich articular cartilage was investigated, using samples from the proximal and distal surfaces of the tarsometatarsus. For morphological analysis, sections were stained with toluidine blue and analyzed by polarized light microscopy. For biochemical analysis, extracellular matrix components were extracted with 4 M guanidinium chloride, fractionated on DEAE-Sephacel and analyzed by SDS-PAGE. Glycosaminoglycans were analyzed by electrophoresis in agarose gels. Structural analysis showed that the fibrils were arranged in different directions, especially on the distal surface. The protein and glycosaminoglycan contents of this region were higher than in the other regions. SDS-PAGE showed the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Articular cartilage; Collagen; Decorin; Ostrich; Proteoglycan.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100007

Osteogenic molecules for clinical applications: improving the BMP-collagen system
Provedor de dados: 48
Autores: Arrabal,Pilar M; Visser,Rick; Santos-Ruiz,Leonor; Becerra,José; Cifuentes,Manuel.
Among the osteogenic growth factors used for bone tissue engineering, bone morphogenetic proteins (BMPs) are the most extensively studied for use in orthopaedic surgery. BMP-2 and BMP-7 have been widely investigated for developing therapeutic strategies and are the only two approved for use in several clinical applications. Due to the chemical and biological characteristics of these molecules, their authorised uses are always in combination with a carrier based on collagen type I. Although the use of these growth factors is considered safe in the short term, the very high doses needed to obtain significant osteoinduction make these treatments expensive and their long-term safety uncertain, since they are highly pleiotropic and have the capacity to induce...
Tipo: Journal article
Palavras-chave: BMPs; Collagen; FGF-2; Growth factors; Osteogenesis; Recombinant proteins; RGD peptide.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400013

Soft Collagen-Gelatine Sponges by Convection Drying
Provedor de dados: 52
Autores: Meyer,Michael; Trommer,Kristin.
This study showed that thermally labile fibrillar collagen could be processed continuously in combination with gelatine as foaming additive by convection drying. The procedure led to stable sponges with similar structural and physical properties as found for freeze-dried collagen samples. The fibrillar collagen remained native, while gelatine acted as foaming additive. The absorbency of the sponges was improved by opening the surface with abrasives. A use as medical device with hemostyptic properties would be possible.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen; Gelatine; Convection drying; Sponge.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100109

Comparison of proximate chemical composition and texture of cupim, Rhomboideus m. and lombo, Longissimus dorsi m. of Nelore (Bos indicus)
Provedor de dados: 52
Autores: Pedrão,Mayka Reghiany; Lassance,Fernanda; Souza,Nilson Evelazio de; Matsushita,Makoto; Telles,Paulo; Shimokomaki,Massami.
The proximate chemical composition of hump, known in Brazil as cupim, Rhomboideus m. (RB), of Nelore (Bos indicus) aged 24 months revealed it to be a unique beef muscle. It presents a lipid fraction 14-fold as high as that of Longissimus dorsi m. (LD) taken from the same animal (p&lt;0.05), the highest value reported so far. This was unequivocally observed by the histological evaluation. Proportionally more protein fraction and conversely less moisture were also observed in RB. Analysis of collagen and its crosslinking with hydroxylysylpyridinium (HP) showed there to be 22.9% more collagen and 14-fold as much HP in RB as in LD. Contrary to the expectations, the tenderness of fresh samples evaluated by Warner Bratzler shear force measurements led to values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zebu breed; Collagen; Hydroxylysylpyridinium; Meat tenderness; Marbling fat.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300023

Histochemical study of fibrillar proteins of the extracellular matrix in benign and malignant mammary neoplasms in dogs
Provedor de dados: 62
Autores: Martins,Ana Maria Cristina Rabello Pinto da Fonseca; Tamaso,Elia; Guerra,José Luiz.
The aim of the present study was to study some of the proteins that form the extracellular matrix of 54 benign and malignant mammary neoplasms in dogs, using histochemical methods: Picrosirius and polarization microscopy for collagenous fibers, Gordon -Sweats's method for reticular fibers and Weigert's fucsine-resorcine method for elastic fibers. A large variability in quantity, distribution and characteristics of the matrix components was observed in the different types of neoplasms. Collagen type I, III and elements of the elastic system had different distribution in benign and malignant neoplasms. The simple Picrossirius method and under polarization enabled visualization of collagen as thick fibers irregularly distributed in the stroma of carcinomas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mammary neoplasms; Collagen; Dogs; Extracellular matrix.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100008

Corticosteroids in acute respiratory distress syndrome
Provedor de dados: 56
Autores: Fernandes,A.B.S.; Zin,W.A.; Rocco,P.R.M.
Improving the course and outcome of patients with acute respiratory distress syndrome presents a challenge. By understanding the immune status of a patient, physicians can consider manipulating proinflammatory systems more rationally. In this context, corticosteroids could be a therapeutic tool in the armamentarium against acute respiratory distress syndrome. Corticosteroid therapy has been studied in three situations: prevention in high-risk patients, early treatment with high-dose, short-course therapy, and prolonged therapy in unresolving cases. There are differences between the corticosteroid trials of the past and recent trials: today, treatment starts 2-10 days after disease onset in patients that failed to improve; in the past, the corticosteroid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute respiratory distress syndrome; Collagen; Cytokine; Methylprednisolone; Experimental models; Clinical trials.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200003

Adrenomedullin and adrenotensin regulate collagen synthesis and proliferation in pulmonary arterial smooth muscle cells
Provedor de dados: 56
Autores: Li,W.; Kong,Q.Y.; Zhao,C.F.; Zhao,F.; Li,F.H.; Xia,W.; Wang,R.; Hu,Y.M.; Hua,M.
To understand the pathophysiological mechanisms of pulmonary arterial smooth muscle cell (PASMC) proliferation and extracellular-matrix accumulation in the development of pulmonary hypertension and remodeling, this study determined the effects of different doses of adrenomedullin (ADM) and adrenotensin (ADT) on PASMC proliferation and collagen synthesis. The objective was to investigate whether extracellular signal-regulated kinase (ERK1/2) signaling was involved in ADM- and ADT-stimulated proliferation of PASMCs in 4-week-old male Wistar rats (body weight: 100-150 g, n=10). The proliferation of PASMCs was examined by 5-bromo-2-deoxyuridine incorporation. A cell growth curve was generated by the Cell Counting Kit-8 method. Expression of collagen I,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenomedullin; Adrenotensin; Pulmonary arterial smooth muscle cell; Extracellular signal-regulated kinase-1; Collagen.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201047

Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
Provedor de dados: 56
Autores: Vesentini,G.; Marini,G.; Piculo,F.; Damasceno,D.C.; Matheus,S.M.M.; Felisbino,S.L.; Calderon,I.M.P.; Hijaz,A.; Barbosa,A.M.P.; Rudge,M.V.C.
The urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Pregnancy; Collagen; Skeletal muscle; Rats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400611

Influence of He-Ne laser therapy on the dynamics of wound healing in mice treated with anti-inflammatory drugs
Provedor de dados: 56
Autores: Gonçalves,W.L.S.; Souza,F.M.; Conti,C.L.; Cirqueira,J.P.; Rocha,W.A.; Pires,J.G.P.; Barros,L.A.P.; Moysés,M.R.
We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in mice treated with steroidal and non-steroidal anti-inflammatory agents. Male albino mice, 28-32 g, were randomized into 6 groups of 6 animals each: control (C), He-Ne laser (L), dexamethasone (D), D + L, celecoxib (X), and X + L. D and X were injected im at doses of 5 and 22 mg/kg, respectively, 24 h before the experiment. A 1-cm long surgical wound was made with a scalpel on the abdomens of the mice. Animals from groups L, D + L and X + L were exposed to 4 J (cm²)-1 day-1 of He-Ne laser for 12 s and were sacrificed on days 1, 2, or 3 after the procedure, when skin samples were taken for histological examination. A significant increase of collagen synthesis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photo-stimulation; Skin; Collagen; Cyclooxygenase; Corticosteroids; Cicatrization; Anti-inflammatory drugs.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600018

Modulation of extracellular matrix by nutritional hepatotrophic factors in thioacetamide-induced liver cirrhosis in the rat
Provedor de dados: 56
Autores: Guerra,R.R.; Trotta,M.R.; Parra,O.M.; Avanzo,J.L.; Bateman,A.; Aloia,T.P.A.; Dagli,M.L.Z.; Hernandez-Blazquez,F.J.
Nutritional substances associated to some hormones enhance liver regeneration when injected intraperitoneally, being denominated hepatotrophic factors (HF). Here we verified if a solution of HF (glucose, vitamins, salts, amino acids, glucagon, insulin, and triiodothyronine) can revert liver cirrhosis and how some extracellular matrices are affected. Cirrhosis was induced for 14 weeks in 45 female Wistar rats (200 mg) by intraperitoneal injections of thioacetamide (200 mg/kg). Twenty-five rats received intraperitoneal HF twice a day for 10 days (40 mL·kg-1·day-1) and 20 rats received physiological saline. Fifteen rats were used as control. The HF applied to cirrhotic rats significantly: a) reduced the relative mRNA expression of the genes: Col-α1 (-53%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver cirrhosis; Hepatotrophic factors; Collagen; Matrix modulation; Progranulin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100005

Collagens and proteoglycans of the corneal extracellular matrix
Provedor de dados: 56
Autores: Michelacci,Y.M.
The cornea is a curved and transparent structure that provides the initial focusing of a light image into the eye. It consists of a central stroma that constitutes 90% of the corneal depth, covered anteriorly with epithelium and posteriorly with endothelium. Its transparency is the result of the regular spacing of collagen fibers with remarkably uniform diameter and interfibrillar space. Corneal collagen is composed of heterotypic fibrils consisting of type I and type V collagen molecules. The cornea also contains unusually high amounts of type VI collagen, which form microfibrillar structures, FACIT collagens (XII and XIV), and other nonfibrillar collagens (XIII and XVIII). FACIT collagens and other molecules, such as leucine-rich repeat proteoglycans,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornea; Proteoglycan; Glycosaminoglycan; Collagen; Dermatan sulfate; Keratan sulfate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800009

Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats
Provedor de dados: 56
Autores: Gianelo,M.C.S.; Polizzelo,J.C.; Chesca,D.; Mattiello-Sverzut,A.C.
The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Vimentin; Desmin; Intermittent stretching; Collagen.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200603

Collagen arrangement in hepatic granuloma in mice infected with Schistosoma mansoni: dependence on fiber radiation centers
Provedor de dados: 56
Autores: Lenzi,H.L.; Kimmel,E.; Schechtman,H.; Pelajo-Machado,M.; Vale,B.S.; Panasco,M.S.; Lenzi,J.A.
The collagen structure of isolated and in situ liver granuloma from Swiss Webster mice infected with Schistosoma mansoni was sequentially and three-dimensionally analyzed during different times of infection (early acute, acute, transitional acute-chronic, and chronic phases) by laser scanning confocal microscopy and electron scanning variable vacuum microscopy. The initial granuloma structure is characterized by vascular collagen residues and by anchorage points (or fiber radiation centers), from where collagenous fibers are angularly shed and self-assembled. During the exudative-productive stage, the self-assembly of these fibers minimizes energy and mass through continuous tension and focal compression. The curvature or angles between collagen fibers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Granuloma; Schistosoma mansoni; Collagen; Extracellular matrix; Confocal microscopy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500018

Supraorganized collagen enhances Schwann cell reactivity and organization in vitro
Provedor de dados: 56
Autores: Maturana,L.G.; Zanon,R.G.; Pierucci,A.; Vidal,B.C.; Oliveira,A.L.R.
We investigated the reactivity and expression of basal lamina collagen by Schwann cells (SCs) cultivated on a supraorganized bovine-derived collagen substrate. SC cultures were obtained from sciatic nerves of neonatal Sprague-Dawley rats and seeded on 24-well culture plates containing collagen substrate. The homogeneity of the cultures was evaluated with an SC marker antibody (anti-S-100). After 1 week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, S-100 and p75NTR (pan neurotrophin receptor) and for scanning electron microscopy (SEM). Positive labeling with antibodies to the cited molecules was observed, indicating that the collagen substrate stimulates SC alignment and adhesion (collagen IV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S-100; P75NTR; Type IV collagen; Collagen; Cell culture; Supraorganized collagen.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700011

Review of the Y chromosome and hypertension
Provedor de dados: 56
Autores: Ely,D.; Turner,M.; Milsted,A.
The Y chromosome from spontaneously hypertensive rats (SHR) has a locus that raises blood pressure 20-25 mmHg. Associated with the SHR Y chromosome effect is a 4-week earlier pubertal rise of testosterone and dependence upon the androgen receptor for the full blood pressure effect. Several indices of enhanced sympathetic nervous system (SNS) activity are also associated with the SHR Y chromosome. Blockade of SNS outflow reduced the blood pressure effect. Salt sensitivity was increased by the Y chromosome as was salt appetite which was SNS dependent. A strong correlation (r = 0.57, P&amp;lt;0.001) was demonstrable between plasma testosterone and angiotensin II. Coronary collagen increased with blood pressure and the presence of the SHR Y chromosome. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sympathetic nervous system; Testosterone; Androgen receptor; Collagen.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600009

Inactivation of capsaicin-sensitive nerves reduces pulmonary remodeling in guinea pigs with chronic allergic pulmonary inflammation
Provedor de dados: 56
Autores: Prado,C.M.; da Rocha,G.Z.; Leick-Maldonado,E.A.; Starling,C.M.; Capelozzi,V.L.; Martins,M.A.; Tibério,I.F.L.C.
Pulmonary remodeling is an important feature of asthma physiopathology that can contribute to irreversible changes in lung function. Although neurokinins influence lung inflammation, their exact role in the extracellular matrix (ECM) remodeling remains to be determined. Our objective was to investigate whether inactivation of capsaicin-sensitive nerves modulates pulmonary ECM remodeling in animals with chronic lung inflammation. After 14 days of capsaicin (50 mg/kg, sc) or vehicle administration, male Hartley guinea pigs weighing 250-300 g were submitted to seven inhalations of increasing doses of ovalbumin (1, 2.5, and 5 mg/mL) or saline for 4 weeks. Seventy-two hours after the seventh inhalation, animals were anesthetized and mechanically ventilated and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen; Elastic fibers; Capsaicin; Chronic lung inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200007

Collagen content, but not the ratios of collagen type III/I mRNAs, differs among hypertensive, alcoholic, and idiopathic dilated cardiomyopathy
Provedor de dados: 56
Autores: Soufen,H.N.; Salemi,V.M.C.; Aneas,I.M.S.; Ramires,F.J.A.; Benício,A.M.D.; Benvenuti,L.A.; Krieger,J.E.; Mady,C.
Cardiac interstitial fibrosis may contribute to ventricular dysfunction and the prognosis of patients with dilated cardiomyopathy. The objective of the present study was to determine if total myocardial collagen content and collagen type III/I (III/I ratio) mRNAs differ in hypertensive, alcoholic, and idiopathic dilated cardiomyopathy subjects. Echocardiography and exercise cardiopulmonary testing were performed in patients with idiopathic (N = 22), hypertensive (N = 12), and alcoholic (N = 11) dilated cardiomyopathy. Morphometric analysis of collagen was performed in fragments obtained by endomyocardial biopsy with picrosirius red staining. The collagen III/I ratio was determined by reverse transcription polymerase chain reaction. Samples of controls (N =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcohol; Heart failure; Hypertension; Dilated cardiomyopathy; Collagen.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200009

Effect of an aqueous extract of Phaseolus vulgaris on the properties of tail tendon collagen of rats with streptozotocin-induced diabetes
Provedor de dados: 56
Autores: Pari,L.; Venkateswaran,S.
Changes in the structural and functional properties of collagen caused by advanced glycation might be of importance for the etiology of late complications in diabetes. The present study was undertaken to investigate the influence of oral administration of aqueous pod extract (200 mg/kg body weight) of Phaseolus vulgaris, an indigenous plant used in Ayurvedic Medicine in India, on collagen content and characteristics in the tail tendon of streptozotocin-diabetic rats. In diabetic rats, collagen content (117.01 ± 6.84 mg/100 mg tissue) as well as its degree of cross-linking was increased, as shown by increased extent of glycation (21.70 ± 0.90 µg glucose/mg collagen), collagen-linked fluorescence (52.8 ± 3.0 AU/µmol hydroxyproline), shrinkage temperature...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen; Diabetes; Phaseolus vulgaris; Cross-linking; Glibenclamide; Collagen glycation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700006

An ultrasound and histomorphological analysis of experimental liver cirrhosis in rats
Provedor de dados: 56
Autores: Dias,J.V.; Paredes,B.D.; Mesquita,L.F.Q.; Carvalho,A.B.; Kozlowski,E.O.; Lessa,A.S.; Takiya,C.M.; Resende,C.M.C.; Coelho,H.S.M.; Campos-de-Carvalho,A.C.; Rezende,G.F.M.; Goldenberg,R.C.S.
We investigated whether liver injury by dual exposure to ethanol and carbon tetrachloride (EtOH + CCl4) for 15 weeks would persist after hepatotoxic agents were removed (EtOH + CCl4/8wR). After 15 weeks of hepatic injury with ethanol (5.5%, m/v) and carbon tetrachloride (0.05, mL/kg, ip), 5 of 11 female Wistar rats were sacrificed. The other 6 rats were maintained for an additional 8 weeks without hepatotoxic agents. Ultrasonography showed increased liver echogenicity and dilation of portal vein caliber in both groups (EtOH + CCl4: 0.22 ± 0.01 cm, P &lt; 0.001; EtOH + CCl4/8wR: 0.21 ± 0.02 cm, P &lt; 0.01) vs control (0.16 ± 0.02 cm). Histopathology showed regenerative nodules in both experimental groups. Histomorphometry revealed increased fibrosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat; Cirrhosis; Collagen; Ultrasound; CCl4; Ethanol.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100008

The in vivo effect of L-arginine on skin elasticity in mice
Provedor de dados: 60
Autores: Souza,Ádria do Prado Barros de; Oliveira,Marina Miranda Rocha de; Andrade,Rafael Rocha de; Amorim,Rivadávio Fernandes Batista de; Bocca,Anamélia Lonrezetti; Borin,Maria de Fátima.
ABSTRACT The human skin aging process is a complex mechanism that can be induced both by intrinsic and extrinsic factors. Observations include a decrease in the biosynthetic and proliferative capacity of cells, increased expression of matrix metalloproteinases, reduction in collagen type I expression, and the progressive disappearance of elastic tissue in the papillary dermis. L-arginine, the substrate of nitric oxide synthesis, is involved in angiogenesis and cell proliferation, as well as an indirect precursor of collagen synthesis via the proline pathway. The aim of this study was to examine the tensile strength, histology, and immunohistochemistry of female and male mice skin receiving different concentrations of topically applied L-arginine, in order...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-arginine; L-arginine/in vivo efficacy; Skin aging; Elastin; Collagen; INOS.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300615