Consulta: subjectFacets:"Enzymatic activity"
Registros recuperados: 29
Data/hora: 08/06/2024 13:43:58

Modification of the activity of an alpha-amylase from Bacillus licheniformis by several surfactants
Provedor de dados: 69
Autores: Bravo Rodríguez,Vicente; Jurado Alameda,Encarnación; Martínez Gallegos,Juan Francisco; Reyes Requena,Antonia; García López,Ana Isabel; Sampaio Cabral,Joaquim Manuel; Fernandes,Pedro; Pina da Fonseca,Luis Joaquim.
The influence of different commercial surfactants on the enzymatic activity of a commercial alpha-amylase from Bacillus licheniformis (Termamyl 300 L) has been studied. As non-ionic surfactants, alkyl polyglycosides (Glucopon® 215, Glucopon® 600 and Glucopon® 650) were studied, as were fatty alcohol ethoxylates (Findet 1214N/23 and Findet 10/15), and nonyl phenol ethoxylate (Findet 9Q/21.5NF). Also, an anionic surfactant, linear alkyl benzene sulfonate (LAS) was assayed. In general, none of the non-ionic surfactants studied, except Findet 10/15, vary substantially the enzymatic activity. Findet 10/15 has the strongest hydrophobic character and reduces the enzymatic activity more significantly the greater its concentration. Regarding LAS, this surfactant...
Tipo: Journal article
Palavras-chave: Alpha-amylase; Alkyl polyglycosides; Enzymatic activity; Fatty alcohol ethoxylates; Linear alkyl benzene sulfonate; Nonyl phenol ethoxylate.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500012

Cloning, expression and characterization of alcohol dehydrogenases in the silkworm Bombyx mori
Provedor de dados: 74
Autores: Wang,Nan; Shi,Haifeng; Yao,Qin; Zhou,Yang; Kang,Lequn; Chen,Huiqin; Chen,Keping.
Alcohol dehydrogenases (ADH) are a class of enzymes that catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones, by using either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), as coenzymes. In this study, a short-chain ADH gene was identified in Bombyx mori by 5'-RACE PCR. This is the first time the coding region of BmADH has been cloned, expressed, purified and then characterized. The cDNA fragment encoding the BmADH protein was amplified from a pool of silkworm cDNAs by PCR, and then cloned into E. coli expression vector pET-30a(+). The recombinant His-tagged BmADH protein was expressed in E. coli BL21 (DE3), and then purified by metal chelating affinity chromatography. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5'-RACE PCRADH; Enzymatic activity; Recombinant protein.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200013

Physiological and biochemical changes in lettuce seeds during storage at different temperatures
Provedor de dados: 75
Autores: Catão,Hugo Cesar RM; Gomes,Luiz Antonio Augusto; Guimarães,Renato M; Fonseca,Pedro Henrique F; Caixeta,Franciele; Galvão,Alexandre G.
ABSTRACT Dormancy in lettuce seeds is a process not yet fully understood. High storage temperatures can cause seed dormancy promoting physiological and enzymatic changes. The goal of this study was to investigate the influence of storage period and environments on the quality and dormancy of different lettuce cultivars. We also investigated the biochemical changes. A completely randomized experimental design was used in a 4x8x3 factorial arrangement to evaluate physiological quality on different storage periods (30, 60, 90 and 120 days) and environments (15, 25 and 35°C) of seeds from eight lettuce cultivars (Everglades, Babá de Verão, Elisa, Luiza, Grand Rapids, Hortência, Salinas 88 and Rubete). The biochemical activity was accessed by tetrazolium test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactuca sativa; Thermotolerance; Thermoinhibition; Dormancy; Enzymatic activity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000100118

Etude histoensymologique de la digestion chez Ruditapes philippinarum
Provedor de dados: 5
Autores: Hily, A.
Examinations of alimentary tract and digestive tubules after feeding give evidence of continuous digestive process in juveniles of Ruditapes philippinarum . Histoenzymologic study of digestive tract suggests important intracellular enzymatic activities in stomacal epithelium, digestive gland and intestinal cells. Main part of intracellular digestion occurred in digestive cells by lysosyme associated with vacuolar system.
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Bivalvia; Digestive system; Enzymatic activity; Histology; Digestive glands; Digestion; Enzymes digestives; Digestion; Bivalve.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1180.pdf

Microbial extracellular enzyme detection on agar plates by means of fluorogenic methylum-belliferyl-substrates.
Provedor de dados: 5
Autores: Kim, S; Hoppe, H.
A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts.
Tipo: Text
Palavras-chave: Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-959.pdf

Dynamique et enzymologie de la digestion chez l'huitre Crassostrea gigas
Provedor de dados: 5
Autores: Boucaud, C; Lebesnerais, C; Lubet, P; Lihrmann, I.
Feeding experiments carried out on Crassostrea gigas allowed the authors to set up the stages and the timing of the movement of food throughout the digestive tract. Various activities involved in digestion (glycosidases, esterases, and peptidases) were investigated histochemically in the digestive tract in order to localize the sites of absorption and the sites of protein and carbohydrate digestion. An explicative model of the different stages of digestion is proposed.
Tipo: Text
Palavras-chave: Crassostrea gigas; Bivalvia; Cytology; Histochemistry; Histology; Digestive system; Enzymatic activity; Digestive glands; Digestion; Enzymes; Transit digestif; Digestion; Huitre; Crassostrea gigas.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1179.pdf

Seasonal development of bacterial communities in a coastal marine sediment as related to the input of organic material.
Provedor de dados: 5
Autores: Meyerreil, L; Graf, G.
The seasonal development of benthic communities was followed in a coastal marine sediment of the Kiel Bight (Baltic Sea; FRG). Total benthic biomass, (ATP), bacterial biomass, overall benthic activity (heat production), and enzymatic decomposition rates of carbohydrate (d-amylase activity) followed a seasonal cycle strongly related to certain events in the sediment. Among these, the input of the phytoplankton blooms in autumn and spring, the accumulation of organic material during winter, and changes in the physical characteristics of the sediment turned out to be most important for the development of benthic biomass and activity. Processes within the benthic community occurred in very short time scales (within days).
Tipo: Text
Palavras-chave: Bacteria; Organic matter; Sediments; Bioenergetics; Enzymatic activity; Biomass.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-944.pdf

Ontogeny of the digestive enzyme activity of the Amazonian pimelodid catfish Pseudoplatystoma punctifer (Castelnau, 1855)
Provedor de dados: 5
Autores: Castro-ruiz, Diana; Mozanzadeh, Mansour Torfi; Fernández-méndez, Christian; Andree, Karl B.; García-dávila, Carmen; Cahu, Chantal; Gisbert, Enric; Darias, Maria J.
The aim of the study was to evaluate the functional ontogeny of the digestive system of Pseudoplatystoma punctifer through the analysis of the activity of the main intestinal (alkaline phosphatase, aminopeptidase N, maltase and leucine-alanine peptidase), pancreatic (trypsin, chymotrypsin, total alkaline proteases, bile-salt activated lipase and amylase) and gastric (pepsin) enzymes. Larvae were raised in triplicate in a recirculation system from 4 to 27 days post fertilization (dpf) at an initial density of 90 larvae L−1, 27.8 ± 0.7 °C and 0 L: 24D photoperiod. Larvae were fed from 4 to 17 dpf with Artemia nauplii and weaned onto an experimentally formulated feed (crude protein content ~ 45%; crude fat content ~ 10%; crude carbohydrate ~ 8%) within...
Tipo: Text
Palavras-chave: Pseudoplatystoma punctifer; Ontogeny; Enzymatic activity; Digestive system; Brush border; Pancreatic enzymes; Gastric enzymes.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00479/59082/61723.pdf

Enzymatic polymorphism study as help for constitution of initial broodstock for a new cultivated finfish species.
Provedor de dados: 5
Autores: Renno, J; Luquet, P.
Leporinus friderici is a South American promising species for fish culture. In French Guiana the "morphological species", L. friderici, L. granti, L. lebaili and L. affinis steyermarki are found. Their identification is sometimes difficult on usual criterion. To constitute an initial broodstock of L. friderici from local wild populations, the genetic structure of these species was studied. Fifteen enzyme systems, representing twenty loci, were screened.
Tipo: Text
Palavras-chave: French Guiana; Leporinus; Leporinus granti; Leporinus friderici; Anostomidae; Pisces; Embryos; Mortality; Genetics; Freshwater fish; Rearing; Breeding; Biopolymorphism; Brood stocks; Enzymatic activity.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/acte-1441.pdf

Variations des activites digestives en fonction des facteurs du milieu chez les crustaces
Provedor de dados: 5
Autores: Galgani, Francois; Benyamin, Y; Vanworhmoudt, A; Cecaldi, J.
Proteolytic enzymes of hepatopancreas of six species of peneids (Penaeus kerathurus, P. japonicus, P. monodon, P. stylirostris, P. merguiensis and P. vannamei ) have been studied. Some of their physiological variations have been characterised. Increase in acclimation temperature of crustaceans enhances increase of specific activities of proteases. Variations of salinity does not modify specific activities, nor quantities of trypsin, nor electrophoretic patterns of these different proteases.
Tipo: Text
Palavras-chave: Penaeus kerathurus; Penaeidae; Malacostraca; Diets; Growth; Larval development; Life cycle; Acclimation; Environmental effects; Digestive glands; Enzymatic activity; Salinity effects; Temperature effects; Enzymes; Endocrinology; Digestion; Développement larvaire; Croissance; Cycle d'intermue; Carboxypeptidase A and B; Leucine aminopeptidase; Chymotrypsin; Trypsine; Enzymes digestives; Homarus ganmarus; Palaemon serratus; P. vannamei; P. merguiensis; P. stylirostris; P. monodon; P. japonicus; Penaeus keratkurus.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1195.pdf

Adaptive features of a bacteriolytic enzyme from the style of the mussel Choromytilus meridionalis in response to environmental fluctuations.
Provedor de dados: 5
Autores: Seiderer, L; Robb, F.
The extracellular digestive apparatus of bivalve molluscs consists of a crystalline style containing diverse degradative enzymes which are released during rotation and dissolution of the style. This paper reviews the occurrence of a temperature-regulated bacteriolytic enzyme in the black mussel Choromytilus meridionalis , which is an inhabitant of coastal reefs on the south and south west coast of South Africa. This agent is unable to lyse Micrococcus luteus or Escherichia coli , both of which are susceptible to lysis by a true lysozyme. It appears to have a restricted spectrum of activity which includes both free-living and kelp-associated gram-negative bacteria. In addition, the level of activity varies dramatically according to the environmental water...
Tipo: Text
Palavras-chave: Choromytilus meridionalis; Bivalvia; Digestive system; Bacteria; Upwelling; Environmental effects; Adaptations; Bacteriocides; Marine molluscs; Enzymatic activity.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-991.pdf

Etude de la fraction azotee soluble de l'anchois sale en cours de maturation
Provedor de dados: 5
Autores: Durand, Patrick.
Different parameters have been followed regularly at various times of anchovy (Engraulis encrasicholus ) ripening. Concentration in NaCl and water content as well as values of water activity and pH are constant and remain quite stable during the entire study period. The relatively acid pH value and the quite low measured water activity level created good conditions for preventing microbial putrefaction at room temperature. The free amino acids and non proteinaceous nitrogen increase indicated the modifications occurring in muscular proteins. These changes resulted in an important proteolytic activity modulated by the medium conditions, especially; pH, temperature and ionic strength. The enzymatic activity slowed down regularly with time but was not...
Tipo: Text
Palavras-chave: Enzymatic activity; Nitrogen compounds; PH; Biochemical composition; Cured products.
Ano: 1981
URL: http://archimer.ifremer.fr/doc/1981/publication-1889.pdf

Rhamdia quelen (Quoy &amp;amp; Gaimard, 1824), submitted to a stressful condition: effect of dietary addition of the essential oil of Lippia alba on metabolism, osmoregulation and endocrinology
Provedor de dados: 83
Autores: Souza,Carine de Freitas; Salbego,Joseânia; Gressler,Luciane T.; Golombieski,Jaqueline I.; Ferst,Juliana G.; Cunha,Mauro A.; Heinzmann,Berta M.; Caron,Braulio O.; Glanzner,Werner G.; Gonçalves,Paulo B. D.; Baldisserotto,Bernardo.
ABSTRACT The aim of this study was to evaluate the effect of the essential oil of Lippia alba (EOLA) as a feed additive on ionoregulatory and metabolic parameters and pituitary hormones expression in silver catfish, Rhamdia quelen , submitted to a stressful condition (stocking density of 10.6 kg m-3 and limited space). Fish were fed with different concentrations of EOLA (0.0 - control, 0.25 and 0.50 mL kg food-1) for 20 days. Metabolic parameters were not affected by the diet, with the exception of alanine aminotransferase, which was higher in the liver of fish fed 0.50 mL EOLA kg food-1. Plasma ions and activity of H+-ATPase did not change, but fish fed 0.25 mL EOLA kg food-1 presented higher Na+/K+-ATPase activity. Somatolactin expression in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cortisol; Enzymatic activity; Growth hormone; Somatolactin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000400707

Characterization of a leucine aminopeptidase from Toxoplasma gondii
Provedor de dados: 19
Autores: Jia, Honglin; Nishikawa, Yoshifumi; Luo, Yuzi; Yamagishi, Junya; Sugimoto, Chihiro; Xuan, Xuenan.
The M17 family leucine aminopeptidase (LAP) hydrolyze amino acids from the N-terminus of peptides. Many LAPs from parasitic protozoa including Plasmmodium, Trypanosoma and Leishmania, have been intensely investigated because of their crucial roles in parasite biology. In this study, a functional recombinant Toxoplasma gondii LAP (rTgLAP) was expressed in Escherichia coli and its enzymatic activity against synthetic substrates for aminopeptidase, as well as the cellular localization was determined. Our results indicated that TgLAP is a functional aminopeptidase in the cytoplasma of T. gondii.
Palavras-chave: Toxoplasma gondii; Leucine aminopeptidase; Enzymatic activity.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2822

Phosphites for the control of anthracnose in common bean
Provedor de dados: 86
Autores: Gadaga,Stélio Jorge Castro; Abreu,Mário Sobral de; Resende,Mário Lúcio Vilela de; Ribeiro Júnior,Pedro Martins.
Abstract: The objective of this work was to evaluate the effect of phosphites in the protection of common bean (Phaseolus vulgaris) against anthracnose. Different phosphite formulations were evaluated by quantifying peroxidase and polyphenol oxidase activity, total phenols, and lignin content. The treatments consisted of sprayings, in the V4, R5, and R7 stages, with: the K, Zn, Mn, K+Mn, K+salicyclic acid, and Cu phosphites; salicylic acid; acibenzolar-S-methyl; the fungicide azoxystrobin; besides a control, without sprayings. The area under the disease progress curve was lower in plants that received applications of the K and Mn phosphites, whose values ranged between 74 and 81%, compared with the control. The K, Zn, and K+salicyilic acid phosphites were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colletotrichum limdemuthianum; Phaseolus vulgaris; Alternative control; Enzymatic activity; Phenolic compounds.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000100036

Effect of broiler breeder age on pancreas enzymes activity and digestive tract weight of embryos and chicks
Provedor de dados: 89
Autores: Maiorka,A; Santin,E; Silva,AVF; Routman,KS; Pizauro Jr.,JM; Macari,M.
A study was carried out to evaluate the effect of broiler breeder age on the development of the digestive tract of embryos and chicks. Fertilized eggs Cobb from 30 and 60 week-old broiler breeder was utilized in this experiment. The results showed that eggs from older (60 weeks of age) broiler breeders were heavier (p = 0.001) than those from younger (30 weeks of age) broiler breeder. In addition, older broiler breeder had larger (p = 0.001) embryos showing a higher yolk sac (p = 0.001) and higher gastrointestinal tract relative weight (p = 0.007) than those from younger broiler breeder. The activities of pancreatic lipase and trypsin enzymes were also higher in embryos from older broiler breeder than those from younger broiler breeder (p = 0.001 and p =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler breeder age; Embryo; Enzymatic activity; Gastrointestinal tract.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100003

Effect of ph and temperature on the activity of phytase products used in broiler nutrition
Provedor de dados: 89
Autores: Naves,L de P; Corrêa,AD; Bertechini,AG; Gomide,EM; Santos,CD dos.
The activity of three commercial microbial phytase (Aspergillus oryzae, A. niger, and Saccharomyces cerevisae) products used in broiler nutrition was determined at different pH (2.0 to 9.0) and temperature (20 to 90°C) values. Enzymatic activity was determined according to the reaction of the phytase with its substrate (sodium phytate), in four replicates, and was expressed in units of phytase activity (FTU). A. oryzae phytase exhibited optimal activity at pH 4.0 and 40°C, but its absolute activity was the lowest of the three phytases evaluated. A. niger phytase exhibited maximal activity close to pH 5.0 and 45ºC, whereas S. cerevisae phytase presented its highest activity at pH close to 4.5 and temperatures ranging between 50 and 60°C. It was concluded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic activity; Enzyme; Phytate; Phytic acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000300004

Chemical and Biochemical Properties of Oxisols after Sewage Sludge Application for 16 Years
Provedor de dados: 90
Autores: Yada,Marcela Midori; Melo,Wanderley José de; Mingotte,Fábio Luiz Checchio; Melo,Valéria Peruca de; Melo,Gabriel Maurício Peruca de.
ABSTRACT The large production of sewage sludge (SS), especially in large urban centers, has led to the suggestion of using this waste as fertilizer in agriculture. The economic viability of this action is great and contributes to improve the environment by cycling the nutrients present in this waste, including high contents of organic matter and plant nutrients. This study evaluated the chemical and biochemical properties of Dystrophic and EutroferricLatossolos Vermelhos (Oxisols) under corn and after SS application at different rates for 16 years. The field experiment was carried out in Jaboticabal, São Paulo State, Brazil, using a randomized block design with four treatments and five replications. Treatments consisted of control - T1 (mineral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soil fertility; Enzymatic activity; Soil basal respiration; Microbial biomass; Urban waste.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501303

Antioxidant enzymes and antioxidant activity in two soursop selections (Annona muricata L.) from Nayarit,Mexico stored at 15 °C
Provedor de dados: 94
Autores: Balois-Morales,Rosendo; Jiménez-Zurita,José Orlando; Alia-Tejacal,Irán; López-Guzmán,Graciela Guadalupe; Palomino-Hermosillo,Yolotzin Apatzingán; Sánchez-Herrera,Leticia Mónica.
Abstract  The changes in concentration of vitamin C, enzymatic and antioxidant activity during the ripening of two soursop selections (G1 and G2) at room temperature (22 ºC) and refrigeration (15 ºC) with an HR 85% were evaluated. The content of soluble protein, the activity of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), the concentration of vitamin C, as well as the antioxidant activity was evaluated by DPPH, ABTS and FRAP methods. The initial soluble protein concentration of the G1 and G2 selections diminished at 22 and 15 °C during ripening. Fruits stored at 22 °C showed the highest CAT activity. The maximum activity of SOD was recorded on the sixth and fourth day in fruits stored at 22 and 15 ºC, respectively. Fruits stored at 22 °C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annona muricata; Ripening; Enzymatic activity; Antioxidant activity; Refrigeration.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000100303

Oxidative metabolism altered by plant growth regulators in atemoia seeds (Annona x atemoya Mabb.) ‘Thompson’
Provedor de dados: 94
Autores: Hata,Ana Paula Yuki; Ferreira,Gisela; Sousa,Marília Caixeta; Bezerra,Breno Kennedy Lima; Kissmann,Camila.
Abstract  The objective of this work was to validate the enzymatic activity in Annona x atemoyaseeds submitted to treatments with plant growth regulators belonging to the group of gibberellins and cytokinins for overcoming dormancy during the germination process. Initially, the water acquisition curve was determined, where two points of phase II (72 and 144 hours) were determined to evaluate the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enzymes in atemoya seeds after the application of two plant growth regulators. Treatments with GA resulted in higher activity of SOD enzyme, which may be favored the higher germination percentage and speed in comparison to control treatment. In cases in which SOD enzyme presented high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germination; Annonaceae; Enzymatic activity; Gibberellin; Cytokinin.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452021000102001

Influence of season, environment and feeding habits on the enzymatic activity of peptidase and β-glucosidase in the gastrointestinal tract of two Siluriformes fishes (Teleostei)
Provedor de dados: 103
Autores: Duarte,Silvana; Paiva,Marco A. R.; Lara,Camila C.; Bemquerer,Marcelo P.; Araújo,Francisco Gerson.
The enzymatic activities involved in the digestion of proteins and carbohydrates were compared among three organs of the digestive track of two Siluriformes fish species, and between two areas: a reservoir, and an area downriver of it. Our aim was to test the hypothesis that the digestive organs of species with varied feeding habits have different enzymatic activities, and that the enzymatic activity differs among seasons and environmental conditions. The iliophagous/herbivorous species Hypostomus auroguttatus Kner, 1854 had higher trypsin-like, chymotrypsin-like peptidase and β-glucosidase activity in the intestine when compared with the omnivorous species Pimelodus maculatus Lacepède, 1803, whereas the latter had more hepatic trypsin-like activity than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic activity; Fishes; Physiology; Reservoirs; Siluriformes; Peptidases; Β-glucosidase.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702013000300006

Enzymatic regulation of photosynthetic and light-independent carbon fixation in Laminaria setchellii (Phaeophyta), Ulva lactuca (Chlorophyta) and Iridaea cordata (Rhodophyta)
Provedor de dados: 97
Autores: CABELLO-PASINI,ALEJANDRO; ALBERTE,RANDALL S.
Carbon is acquired through photosynthetic and non-photosynthetic processes in marine algae. However, little is known about the biochemical regulation of these metabolic pathways along the thallus of seaweeds. Consequently, the objective of this study was to assess the distribution of in vivo carboxylation pathways and to relate them to the in vitro activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO), phosphoenolpyruvate carboxykinase (PEPCK), and phosphoenolpyruvate carboxylase (PEPC) in the Phaeophyte Laminaria setchellii, the Chlorophyte Ulva lactuca, and the Rhodophyte Iridaea cordata. Chlorophyll-a levels did not vary in U. lactuca and I. cordata. However, pigment levels were significantly lower in the meristematic region of L....
Tipo: Journal article
Palavras-chave: Enzymatic activity; Chlorophyll; Light-independent carbon fixation; Photosynthesis.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000200002

Root phosphatase activity, plant growth and phosphorus accumulation of maize genotypes
Provedor de dados: 63
Autores: Machado,Cynthia Torres de Toledo; Furlani,Ângela Maria Cangiani.
The activity of the enzyme phosphatase (P-ase) is a physiological characteristic related to plant efficiency in relation to P acquisition and utilization, and is genetically variable. As part of a study on maize genotype characterization in relation to phosphorus (P) uptake and utilization efficiency, two experiments were set up to measure phosphatase (P-ase) activity in intact roots of six local and improved maize varieties and two sub-populations. Plants were grown at one P level in nutrient solution (4 mg L-1) and the P-ase activity assay was run using 17-day-old plants for varieties and 24-day-old plants for subpopulations. Shoot and root dry matter yields and P concentrations and contents in plant parts were determined, as well as P-efficiency...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays L.; Genetic variability; Enzymatic activity; Nutritional efficiency.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000200015

Acid phosphatase activity and leaf phosphorus content in soybean cultivars
Provedor de dados: 63
Autores: Raposo,Roberto Wagner Cavalcanti; Muraoka,Takashi; Basso,Luiz Carlos; Lavres Jr.,José; Franzini,Vinicius Ide.
The phosphate fertilization represents the most costly fraction of soybean crop production. Efficient soybean cultivars for P absorption and utilization in soils of medium available P are highly desirable and might contribute for increasing crop production potential. Thirty two soybean [Glycine max (L.) Merr.] cultivars recommended for 'Cerrado' and differing in growth cycle (early, semi-early, semi-late, and late) were grown in a dystrophic Typic Haplustox Cerrado soil to evaluate the acid phosphatase activity, P content in the diagnostic leaf, and shoot biomass. There were differences among the soybean cultivars within all maturation groups in acid phosphatase activity and shoot biomass. The diagnostic-leaf P-content showed significant differences on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic activity; Diagnostic-leaf; Maturation group.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400014

β-galactosidase Encapsulated in Carrageenan, Pectin and Carrageenan/Pectin: Comparative Study, Stability and Controlled Release
Provedor de dados: 42
Autores: SILVA,RENATA CRISTINA; TREVISAN,MARCELLO G.; GARCIA,JERUSA SIMONE.
Abstract  The present study investigated the encapsulation of β-galactosidase in carrageenan, pectin and its hybrid hydrogels by using the ionotropic gelation method. The material obtained was characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG/DTG) and scanning electron microscopy (SEM). The effects of pH, temperature and storage time were evaluated in terms of the catalytic activity of the free and encapsulated enzyme. Addition studies were conducted evaluating the performance of catalytic activity in vitro conditions. Carrageenan, pectin and hybrid hydrogels presented encapsulation efficiency of 58 ± 1%, 72 ± 1% and 77 ± 2%, respectively. The pectin hydrogel showed the higher β-galactosidase activity in pH and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopolymer; Encapsulation; Enzymatic activity; Ionotropic gelation; Lactase.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100905

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation
Provedor de dados: 52
Autores: Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002

Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
Provedor de dados: 52
Autores: Bresolin,Iara Rocha Antunes Pereira; Bresolin,Igor Tadeu Lazzarotto; Silveira,Edgar; Tambourgi,Elias Basile; Mazzola,Priscila Gava.
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromelain; Pineapple peel; Downstream processing; Waste; Therapeutic applications; Enzymatic activity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012

Biological Activity in a Degraded Alfisol Amended with Sewage Sludge and Cropped with Yellow Serradela (Ornithopus compressus L.)
Provedor de dados: 117
Autores: Celis H,José; Machuca H,Angela; Sandoval E,Marco; Morales C,Patricia.
There are few studies about the impact of sewage sludge on the biological properties in Alfisols of the Chilean Coastal Range drylands. Hence, the objective of this study was to evaluate its effect on the microbial respiration and enzymatic activities of a degraded Alfisol located in the Bío Bío Region (Chile) that was cropped with yellow serradela (Ornithopus compressus L.). Sludge was added to the soil at rates of 15, 30, and 60 t ha-1; he following treatments were defined: L15-P = 15 t ha-1 sludge + O. compressus; L30-P = 30 t ha-1 sludge + O. compressus; L60-P = 60 t ha-1 sludge + O. compressus; L15 = 15 t ha-1 sludge; L30 = 30 t ha-1 sludge; L60 = 60 t ha-1 sludge; CP = non-amended soil, cropped; and C = non-amended soil, no crop. Soil microorganism...
Tipo: Journal article
Palavras-chave: Biosolids; Respiration; Enzymatic activity; Degraded soils; Remediation.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100020

Different feeding habits influence the activity of digestive enzymes in freshwater fish
Provedor de dados: 65
Autores: Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia.
ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine.  Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406