Consulta: creatorFacets:"Haffner, Philippe"
Registros recuperados: 46
Data/hora: 01/06/2024 08:23:57

Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1
Provedor de dados: 5
Autores: Segarra, Amelie; Mauduit, Florian; Faury, Nicole; Trancart, Suzanne; Degremont, Lionel; Tourbiez, Delphine; Haffner, Philippe; Barbosa-solomieu, Valerie; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
Background:  Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches.  Results:  The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant...
Tipo: Text
Palavras-chave: Interactions; Ostreid herpesvirus 1; Gene expression; Susceptibility; Crassostrea gigas.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00199/31029/29431.pdf

Real-time PCR assay for rapid detection and quantification of Vibrio aestuarianus in oyster and seawater: A useful tool for epidemiologic studies
Provedor de dados: 5
Autores: Saulnier, Denis; De Decker, Sophie; Haffner, Philippe.
Because Vibrio aestuarianus is known to cause serious infections in Pacific oyster Crassostrea gigas, a real-time PCR assay was developed targeting the dnaJ gene of this bacterium. Only V. aestuarianus strains isolated from C. gigas mortality events in different geographic areas and the reference strain tested positive, whereas no amplification products was obtained with type strains belonging to 23 other species of Vibrio. Sensitivity and reproducibility of the method were assessed using either seawater or oyster homogenate samples spiked with one V aestuarianus strain. All these samples were stored at -20 degrees C in order to mimic retrospective or grouped natural sample analysis without quantification bias due to prolonged freezing. Analysis of...
Tipo: Text
Palavras-chave: V. aestuarianus; Vibrio; Taqman; Real time PCR; Pathogen; Oyster; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6447.pdf

Vibrio damsela as a pathogenic agent causing mortalities in cultured sea bass (Lates calcarifer)
Provedor de dados: 5
Autores: Renault, Tristan; Haffner, Philippe; Malfondet, C.; Weppe, Maurice.
Vibrio anguillarum and Vibrio ordali are species frequently described as fish pathogens. Seven species of Vibrio can also be implicated in disease problems in mariculture (Toranzo  1990). sorne of Vibrios and Barja, In addition, these marine such as V. vulnificus (Tison et al.. 1982) and V. damsela (Love et al., 1981) can  also cause illness homoiothermic animals
Tipo: Text
Palavras-chave: Fish pathogens; Lates calacrifer; Mortality; Vibrio damsela.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-2889.pdf

A field report on vibrio disease of seabass (Dicentrarchus labrax ) in the South of France
Provedor de dados: 5
Autores: Breuil, Gilles; Haffner, Philippe.
Vibrio anguillarum was isolated from diseased seabass reared in aquatic farms (hatcheries and grow-out farms) located along the French Mediterranean coast. A representative strain (V62) differs from the French V. anguillarum strain (V408) by its ability to grow on a 0% salinity medium. The pathogenicity of the representative strain for sea bass was confirmed by injections and bath challenge. The V62 strain reacted with V. anguillarum antiserum (V408) and 84% of the isolated strains from diseased sea bass agglutinated with anti V62 serum.
Tipo: Text
Palavras-chave: MED; France Dicentrarchus labrax Serranidae Pisces Vibrio anguillarum Hatcheries Fish culture Rearing Bacteria Marine aquaculture Marine fish Mortality.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/acte-1490.pdf

Species-specific mechanisms of cytotoxicity toward immune cells determine the successful outcome of Vibrio infections
Provedor de dados: 5
Autores: Rubio, Tristan; Oyanedel, Daniel; Labreuche, Yannick; Toulza, Eve; Luo, Xing; Bruto, Maxime; Chaparro, Cristian; Torres, Marta; De Lorgeril, Julien; Haffner, Philippe; Vidal-dupiol, Jeremie; Lagorce, Arnaud; Petton, Bruno; Mitta, Guillaume; Jacq, Annick; Le Roux, Frederique; Charriere, Guillaume; Destoumieux-garzon, Delphine.
Vibrio species cause infectious diseases in humans and animals, but they can also live as commensals within their host tissues. How Vibrio subverts the host defenses to mount a successful infection remains poorly understood, and this knowledge is critical for predicting and managing disease. Here, we have investigated the cellular and molecular mechanisms underpinning infection and colonization of 2 virulent Vibrio species in an ecologically relevant host model, oyster, to study interactions with marine Vibrio species. All Vibrio strains were recognized by the immune system, but only nonvirulent strains were controlled. We showed that virulent strains were cytotoxic to hemocytes, oyster immune cells. By analyzing host and bacterial transcriptional...
Tipo: Text
Palavras-chave: T6SS; Toxin; Dual RNA-seq; Cytolysis; Pathogenesis.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00507/61837/65900.pdf

Summer mortality outbreaks of French Pacific oysters Crassostrea gigas in 2008 and 2009
Provedor de dados: 5
Autores: Renault, Tristan; Allain, Gwenhael; Arzul, Isabelle; Chollet, Bruno; Cobret, Laetitia; De Decker, Sophie; Faury, Nicole; Ferrand, Sylvie; Francois, Cyrille; Garcia, Celine; Haffner, Philippe; Joly, Jean-pierre; Miossec, Laurence; Morga, Benjamin; Nicolas, Jean-louis; Omnes, Emmanuelle; Pepin, Jean-francois; Saulnier, Denis; Schikorski, David; Segarra, Amelie.
Abnormal mortality outbreaks occurred in the majority of Pacific oyster (Crassostrea gigas) producing areas in France in 2008 during the summer period. These outbreaks were usually sudden and severe (up to 100%) and affected essentially spat (oysters less than 1 year-old) and juveniles (12 to 18 month-old oysters). Crassostrea gigas was the single affected species Mortality outbreaks were first observed from mid May to mid June in Brittany, Vendée, Charente Maritime and Mediterranean coast A second episode of massive mortality events was reported at the end of June and the beginning of July. All oyster production sites were affected except some particular locations including Arcachon Bay and Brittany. Mortality occurred latter in Arcachon Bay in August....
Tipo: Text
Palavras-chave: Abnormal mortality; Oysters; Crassostrea gigas; Pathogens.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00014/12554/9420.pdf

Mass mortalities in hatchery-reared sea bass (Lates calcarifer) larvae associated with the presence in the brain and retina of virus-like particles
Provedor de dados: 5
Autores: Renault, Tristan; Haffner, Philippe; Baudin Laurencien, F.; Breuil, Gilles; Bonami, Jean-robert.
Since 1986, an unknown disease causing high mortalities appeared in Ifremer's Pacific OceanologicaI Center (Tahiti) nursery of sea bass (Lates calcarifer). opathology of lesions involving both centraI nervous system (CNS) and eye retina, and the tuation exists in South-East Asian countries (Indonesia, Philippines, Malaysia and Singapore), and in northem Australia.  This paper describes the histmorphology of a new fish virus, which is believed to be the aetiological agent of high mortalities among sea bass larvae.
Tipo: Text
Palavras-chave: Virus like; Lates calacrifer; Hatchery; Mortality.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/publication-2885.pdf

Ecologically realistic model of infection for exploring the host damage caused by Vibrio aestuarianus
Provedor de dados: 5
Autores: Parizadeh, Leila; Tourbiez, Delphine; Garcia, Celine; Haffner, Philippe; Degremont, Lionel; Le Roux, Frederique; Travers, Marie-agnes.
Although vibrios are frequently associated with marine organisms mortality outbreaks, knowledge on their ecology and pathogenicity is sparse, thus limiting disease management and prophylactic strategies. Here, we investigated V. aestuarianus infection onset and progression in the wild, taking advantage of a “claire” pond: a semi‐closed system with limited seawater renewal, theoretically more adapted to disease transmission. We showed a positive association of the bacteria with oysters, which can constitute a reservoir for the bacteria in the winter. Moreover, passage through oysters was found to be necessary for experimental disease reproduction as vibrios shedding from diseased oysters has higher infectivity than from in vitro grown. We next developed an...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00446/55761/57423.pdf

A single regulatory gene is sufficient to alter Vibrio aestuarianus pathogenicity in oysters
Provedor de dados: 5
Autores: Goudenege, David; Travers, Marie-agnes; Lemire, Astrid; Petton, Bruno; Haffner, Philippe; Labreuche, Yannick; Tourbiez, Delphine; Mangenot, Sophie; Calteau, Alexandra; Mazel, Didier; Nicolas, Jean-louis; Jacq, Annick; Le Roux, Frederique.
Oyster diseases caused by pathogenic vibrios pose a major challenge to the sustainability of oyster farming. In France since 2012 a disease affecting specifically adult oysters has been associated with the presence of Vibrio aestuarianus. Here, by combining genome comparison, phylogenetic analyses and high throughput infections of strains isolated before or during the recent outbreaks, we show that virulent strains cluster into two V. aestuarianus lineages independently of the sampling dates. The bacterial lethal dose was not different between strains isolated before or after 2012. Hence the emergence of a new highly virulent clonal strain is unlikely. Each lineage comprises nearly identical strains, the majority of them being virulent, suggesting that...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00226/33685/32530.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 96 RPC R70 - Programme Pathologie - Rapport Scientifique année 1996
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Lipart, Cecile; Thebault, Anne.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00043/15452/12827.pdf

Rapport de mission en Nouvelle-Calédonie du 6/07 /98 au 13/07 /98
Provedor de dados: 5
Autores: Haffner, Philippe.
Cette mission avait pour objectif de faire un point sur les travaux des six derniers mois, menés au COP et à la SASV, principalement, mais pas exclusivement, autour du "syndrôme 93. Les thèmes milieux, pathologie, immunologie, génétique et zootechnie ont été abordés. Différentes perspectives de recherches et de collaborations sont proposées.
Tipo: Text
Palavras-chave: Penaeus stylirostris; Nouvelle Calédonie; Syndrôme 93.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00680/79228/81742.pdf

Development of an antiseptic procedure to improve retention rates during the grafting process of black-lip oyster, Pinctada margaritifera
Provedor de dados: 5
Autores: Cochennec, Nathalie; Haffner, Philippe; Levy, Peva; Saulnier, Denis; Fougerouse, A.; Langy, Sandra.
In French Polynesia, black pearl cultivation now takes place on about 30 of the 118 atolls or islands. This activity has largely replaced copra, coconut oil and fishing as a source of income. Around 1,000 pearl farms, generally small ones of less than 5 ha, are registered. The pearl farms are mainly owned by families. In 1998, about 7,000 persons were employed in theentire pearl industry, from production to retailing. In value, this activity represents the first exportation of French Polynesian products. From 1988 to date, exports increased exponentially before leveling. Starting with less than two kilograms in 1972, exports have now stabilized at Il tons of "black" pearls.
Tipo: Text
Palavras-chave: Vibrio; Microbiology; Grafting process; Pinctada margaritifera.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-4928.PDF

Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters
Provedor de dados: 5
Autores: De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume.
Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf

Mission du 13-05 au 07-06-90 à l'écloserie Sodacal - Utilisation des AQUA-PLAKr en écloserie industrielle. Rapport final
Provedor de dados: 5
Autores: Haffner, Philippe.
La mission effectuée du 13 mai au 7 juin 90 à l'écloserie de la ferme Sodacal en Nouvelle-Calédonie avait pour but de tester les aqua-plakR pour les comptages bactériens en routine dans une écloserie industrielle. La technique classique (comptage sur boite de Pétri) est en effet très peu utilisée par les éleveurs du fait de sa lourdeur alors qu'une bonne connaissance de l'évolution de la flore bactérienne dans les bacs d'élevages peut s'avérer nécessaire pour assurer une gestion sanitaire correcte de ces élevages. D'autre part, une collection la plus large possible des différentes souches bactériennes rencontrées a été réalisée. Le présent rapport n'aborde que l'aspect utilisation des aqua-plakR, les souches bactériennes étant actuellement en cours...
Tipo: Text
Palavras-chave: Ecloserie; Aqua-PlakR; Comptage bactérien; Nouvelle Calédonie.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00131/24191/22185.pdf

Oyster Farming, Temperature, and Plankton Influence the Dynamics of Pathogenic Vibrios in the Thau Lagoon
Provedor de dados: 5
Autores: Lopez-joven, Maria-carmen; Rolland, Jean-luc; Haffner, Philippe; Caro, Audrey; Roques, Cecile; Carre, Claire; Travers, Marie-agnes; Abadie, Eric; Laabir, Mohamed; Bonnet, Delphine; Destoumieux-garzon, Delphine.
Vibrio species have been associated with recurrent mass mortalities of juvenile oysters Crassostrea gigas threatening oyster farming worldwide. However, knowledge of the ecology of pathogens in affected oyster farming areas remains scarce. Specifically, there are no data regarding (i) the environmental reservoirs of Vibrio populations pathogenic to oysters, (ii) the environmental factors favoring their transmission, and (iii) the influence of oyster farming on the persistence of those pathogens. This knowledge gap limits our capacity to predict and mitigate disease occurrence. To address these issues, we monitored Vibrio species potentially pathogenic to C. gigas in 2013 and 2014 in the Thau Lagoon, a major oyster farming region in the coastal French...
Tipo: Text
Palavras-chave: Vibrio; Shellfish farming; Bivalve mollusks; Mortality outbreak; Phytoplankton; Zooplankton.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00463/57479/59598.pdf

Episodes de mortalité massive de moules bleues observés en 2014 dans les Pertuis charentais
Provedor de dados: 5
Autores: Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan.
Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen
Tipo: Text
Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 95 RPC R 62 - Programme Pathologie - Rapport Scientifique année 1995
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Thuillier, Benoit.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00043/15453/12828.pdf

Surmortalités de la moule bleue Mytilus edulis dans les Pertuis Charentais (mars 2014)
Provedor de dados: 5
Autores: Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre.
Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf

Bilan 2009 du réseau REPAMO - Réseau national de surveillace de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Cobret, Laetitia; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Breerette, Stephane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Banamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00086/19711/17352.pdf

Etude du virus de type herpes observé chez les huîtres
Provedor de dados: 5
Autores: Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Delsert, Claude; Cochennec, Nathalie; Renault, Tristan.
Since 1991, sporadic, but high mortality in larvae of the Pacific oyster (Crassostrea gigas) has been observed during the summer in various French hatcheries. This mortality was concomitant with the detection of a virus by transmission electron microscopy. This virus, by virtue of its morphological characteristics and developmental cycle, seems to belong to the Herpesviridae family. The pathogenicity of this viral agent has been demonstrated in the laboratory by experimentally induced mortality on healthy larvae. Moreover, concomitant mortality has been reported in larvae of Crassostrea gigas and Ostrea edulis reared in the same tanks, with detection of a herpes-type virus.
Tipo: Text
Palavras-chave: Virus; Herpeviridae; Pathologie; Crassostrea gigas.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3248.pdf

Note de synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Avril-Mai 1996
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile; Le Deuff, Rose-marie; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade dans le cadre de l'étude du virus de type herpès et de la mise au point de méthodes de diagnostic fait suite à la précédente note du 16 avril 1996. Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont donnés et commentés en sept points. 1- Analyses Pays de Loire 2 - Bilan des analyses réalisées dans le cadre du protocole IFREMER BOUIN/IFREMER ARGENTON/SODABO 3 - Bilan global des analyses Pays de Loire et Bouin-Argenton-SODABO 4 - Analyses effectuées en PCR pour la recherche de virus de type herpès sur des lots prélevés en 1996, dans le...
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15499/12867.pdf

Bilan 2013 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Tourbiez, Delphine; Chollet, Bruno; Faury, Nicole; Haffner, Philippe; Dubreuil, Christine; Serpin, Delphine; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Cheve, Julien; Penot, Julia; Le Gal, Dominique; Lebrun, Luc; Le Gac-abernot, Chantal; Langlade, Aime; Bedier, Edouard; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrick; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Repamo a pour objectif de détecter précocement les infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage.  L’étude des hausses de mortalités a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la collecte d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités d’huîtres creuses (Crassostrea gigas) (44 interventions) ont été recensées dans la majorité des bassins de production de mai à...
Tipo: Text
Palavras-chave: Réseau Surveillance; Pathologie; Mollusques; Coquillages; Santé.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00197/30798/29167.pdf

Comparative study of bactrial infections responsible for mass mortality in penaeid shrimp hatcheries of the Pacific zone
Provedor de dados: 5
Autores: Le Groumellec, M.; Haffner, Philippe; Martin, B.; Martin, C.
Mass mortality associated with luminescence has been dbserved in sorne Thai and Ecuadorian penaeid shrimp hatcheries in recent years. Samples collected from hatcheries in these two different areas revealed luminescent sucrose-negative Vibrio strains in larVal rearing tanks where mortality occurred. Pathogenicity trials were carried out to identify the causative agents of mortality and to study the course of infection . . Results showed that strains isolated from Thai hatclleries were more virulent than Ecuadorian strains when tested on different larval stages of Penaeus monodon. The virulence of these strains was also estimated. Trials on other species ofpenaeid shrimp demonstrated the specifie action of Thai strains. Drug sensitivity patterns revealed a...
Tipo: Text
Palavras-chave: Mass mortality; Bacterial; Vibrio; Hatcheries; Penaeus monodon; Shrimp.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/1993/acte-6100.PDF

Expression de gènes viraux de l'herpès virus OsHV-1 sous sa forme µVar chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Segarra, Amelie; Faury, Nicole; Haffner, Philippe; Pepin, Jean-francois; Tourbiez, Delphine; Trancart, Suzanne; Travers, Marie-agnes; Renault, Tristan.
Le premier cas d'infection à virus de type herpès a été rapporté en 1972, aux Etats Unis, chez l'huître américaine, Crassostrea virginica (Farley et al, 1972). A partir de 1991, des mortalités massives associées à la détection de virus de type herpes ont été observées chez plusieurs espèces de bivalves marins, à différents stades de développement (essentiellement chez les larves et le naissain) et dans différentes régions du monde. Sur la base d’observation en microscopie électronique à transmission, ces virus présentaient des caractéristiques morphologiques identiques à celles des Herpesvirus (Roizman, 1982).  Les connaissances acquises concernant la taille, la structure et le séquençage du génome d’un virus infectant les larves d’huîtres creuses en...
Tipo: Text
Palavras-chave: Herpès virus OsHV-1; Expression gènes; Huître creuse; Crassostrea gigas.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00195/30627/29076.pdf

Que savons-nous des Marteillia?
Provedor de dados: 5
Autores: Berthe, Franck; Haffner, Philippe; Zerabib, Maamar; Rodriguez, David.
Marteilia refringens, a parasitic protist, is partially responsible for the drastic reduction in French production of the European flat oyster (Ostrea edulis) in the 1970s and still constitutes a major obstacle for revitalizing coastal farming of this species.
Tipo: Text
Palavras-chave: Bivalves; Ostrea edulis; Protiste; Parasite; Marteilia refringens; Pathologie.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3247.pdf

Bilan 2012 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Pepin, Jean-francois; Hatt, Philippe-jacques; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Huchet, Eve; Dubreuil, Christine; Chollet, Bruno; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Annezo, Jean-pierre; Terre-terrillon, Aouregan; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Hittier, Benoist; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à réglementés et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font partie des missions institutionnelles de l’IFREMER.   L’étude des hausses de mortalités (protocole II) a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la réalisation d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités...
Tipo: Text
Palavras-chave: Réseau; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00143/25470/23625.pdf

A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors
Provedor de dados: 5
Autores: Nasfi, H.; Travers, Marie-agnes; De Lorgeril, Julien; Habib, C.; Sannie, T.; Sorieul, L.; Gerard, J.; Avarre, J. C.; Haffner, Philippe; Tourbiez, Delphine; Renault, Tristan; Furones, D.; Roque, A.; Pruzzo, C.; Cheslett, D.; Gdoura, R.; Vallaeys, T.
The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase...
Tipo: Text
Palavras-chave: VNTR; Vibrio splendidus; Epidemiology; Vsm; OmpU; Crassotrea giga; Mortality.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00255/36666/35350.pdf

Vibrio splendidus   O-antigen structure: A trade-off between virulence to oysters and resistance to grazers
Provedor de dados: 5
Autores: Oyanedel, Daniel; Labreuche, Yannick; Bruto, Maxime; Amraoui, Hajar; Robino, Etienne; Haffner, Philippe; Rubio, Tristan; Charrière, Guillaume; Le Roux, Frederique; Destoumieux-garzón, Delphine.
A major debate in evolutionary biology is whether virulence is maintained as an adaptive trait and/or evolves to non‐virulence. In the environment, virulence traits of non‐obligatory parasites are subjected to diverse selective pressures and trade‐offs. Here we focus on a population of Vibrio splendidus that displays moderate virulence for oysters. A MARTX (Multifunctional‐autoprocessing repeats‐in‐toxin) and a type‐six secretion system (T6SS) were found to be necessary for virulence toward oysters, while a region (wbe) involved in O‐antigen synthesis is necessary for resistance to predation against amoebae. Gene inactivation within the wbe region had major consequences on the O‐antigen structure, conferring lower immunogenicity, competitive advantage and...
Tipo: Text
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00619/73070/72194.pdf

Programme Pathologie - Rapport scientifique Année 1994 - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 94 RPC R 62
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno; Maffart, Patricia; Haffner, Philippe; Berthe, Franck; Thuillier, Benoit.
Le but de ce programme pathologie est de recherher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassastrea gigas, afin d'identifier d'éventuels agents causant des mortalité, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socioéconomique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la pathologie....
Tipo: Text
Palavras-chave: Pathologie; Agent pathogène; Infection; Virus; Herpèsvirus; Purification; Huître creuse; Crassostrea gigas.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00044/15502/12874.pdf

Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Travers, Marie-agnes; Tourbiez, Delphine; Parizadeh, Leila; Haffner, Philippe; Kozic-djellouli, Angelique; Aboubaker, Mohamed; Koken, Marcel; Degremont, Lionel; Lupo, Coralie.
This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00386/49768/50308.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 98 RPC R 7 - Programme Pathologie - Rapport Final année 1998
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf

Mortalités massives de l'huître creuse - Rapport final du programme de recherche sur l'étude de la cinétique de détection d'agents infectieux associés à des épisodes de mortalités de naissains d'huîtres creuses Crassostrea gigas sur un site ostréicole de Marennes-Oléron (CIDAGINF 2009-2010)
Provedor de dados: 5
Autores: Saulnier, Denis; Pepin, Jean-francois; Guesdon, Stephane; Degremont, Lionel; Faury, Nicole; Haffner, Philippe; Renault, Tristan; Travers, Marie-agnes; Tourbiez, Delphine; Geairon, Philippe; Le Moine, Olivier; Seugnet, Jean-luc; Soletchnik, Patrick.
This study aims to improve understanding of causes of mortalities in oyster Crassostrea gigas in pertuis charentais related to environmental parameters and the kinetics of targeted infectious agents, such as bacteria Vibrio splendidus (group ) and V. aestuarianus and Ostreid herpesvirus OsHV-1. Two sampling campaigns were carried out on the site of Agnas in 2009 and 2010, before, during or following mortality events of spat from March to September. The approach is to detect and quantify in short time step infectious agents from tissues of farmed oysters placed on rack and in various samples taken nearby: native oysters and other species (wild mussels and barnacles), seawater and sediment. With physical and chemical automatic probes placed on the site,...
Tipo: Text
Palavras-chave: Huître creuse; Crassostrea gigas; Mortalité; Virus herpès; Vibrions; Environnement; Pertuis Charentais; Pacific oyster; Crassostrea gigas; Mortality; Herpes virus; Vibrios; Environment; Pertuis Charentais.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00058/16920/14400.pdf

Bilan 2008 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Tourbiez, Delphine; Omnes, Emmanuelle; Cobret, Laetitia; Faury, Nicole; Haffner, Philippe; Saulnier, Denis; Pepin, Jean-francois; Renault, Tristan; Antajan, Elvire; Rauflet, Fabienne; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00086/19709/17351.pdf

Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome
Provedor de dados: 5
Autores: De Lorgeril, Julien; Petton, Bruno; Lucasson, Aude; Perez, Valérie; Stenger, Pierre-louis; Degremont, Lionel; Montagnani, Caroline; Escoubas, Jean Michel; Haffner, Philippe; Allienne, Jean-françois; Leroy, Marc; Lagarde, Franck; Vidal Dupiol, Jeremie; Gueguen, Yannick; Mitta, Guillaume.
Background: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein...
Tipo: Text
Palavras-chave: Pacific oyster; Oyster disease; Resistance; OsHV-1; Antiviral molecular pathways; Invertebrate immunity.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00602/71369/69811.pdf

A Sustained Immune Response Supports Long-Term Antiviral Immune Priming in the Pacific Oyster,  Crassostrea gigas
Provedor de dados: 5
Autores: Lafont, Maxime; Vergnes, Agnes; Vidal-dupiol, Jeremie; De Lorgeril, Julien; Gueguen, Yannick; Haffner, Philippe; Petton, Bruno; Chaparro, Cristian; Barrachina, Celia; Destoumieux-garzon, Delphine; Mitta, Guillaume; Gourbal, Benjamin; Montagnani, Caroline.
Over the last decade, innate immune priming has been evidenced in many invertebrate phyla. If mechanistic models have been proposed, molecular studies aiming to substantiate these models have remained scarce. We reveal here the transcriptional signature associated with immune priming in the oyster Crassostrea gigas. Oysters were fully protected against Ostreid herpesvirus 1 (OsHV-1), a major oyster pathogen, after priming with poly(I·C), which mimics viral double-stranded RNA. Global analysis through RNA sequencing of oyster and viral genes after immune priming and viral infection revealed that poly(I·C) induces a strong antiviral response that impairs OsHV-1 replication. Protection is based on a sustained upregulation of immune genes, notably genes...
Tipo: Text
Palavras-chave: Innate immunity; Priming; OsHV-1; Antiviral response; Immune memory; Oyster; POMS; Poly(I·C); Interferon; Transcriptomic.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00614/72580/71541.pdf

Bilan 2010 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Guichard, Benjamin; Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Brerette, Stéphane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam; Cantin, Christian.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l’IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l’huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00059/17050/14599.pdf

Synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Juin-Septembre 1996
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type . herpès et de la mise au point des méthodes de diagnostic fait suite à la précédente note (fin mai 1996). Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont données et commentés en quatre points. Cette étude est soutenue par la Région Poitou-Charente et la Région Pays de Loire.
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Infection; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15503/12875.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 97 RPC R 58 - Programme Pathologie - Rapport Scientifique année 1997
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf

Histological abnormalities related to mortality in penaeid shrimp (Penaeus stylirostris) reared in New-Caledonia
Provedor de dados: 5
Autores: Berthe, Franck; Legroumellec, Marc; Haffner, Philippe; Renault, Tristan.
Outbreaks of mass mortality occurred since 1993 in penaeid shrimp (Penaeus stylirostris) reared in New-Caledonia. Since then, the disease, called "syndrome 93" (S93) has inflicted serious losses (60 to 100%) during the cold period and thus must be considered as an economically significant disease.
Tipo: Text
Palavras-chave: Penaeus stylirostris; Penaeid shrimp; Mortality; Histology.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2974.pdf

Programme Herpèsvirus. Synthèse des travaux réalisés au Laboratoire IFREMER de La Tremblade - Mars 1996 - Février 1997
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Ce rapport représente la synthèse des travaux réalisés de mars 1996 à février 1997 au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type herpès observé chez les huîtres et de la mise au point de méthodes de diagnostic. La majorité des travaux rapportés ici de façon complète, a déjà fait l'objet de comptes rendus intermédiaires.
Tipo: Text
Palavras-chave: Pathologie; Herpèsvirus; Herpesviridae; Huître creuse; Crassostrea gigas; Huître plate; Ostrea edulis; Virus; Détection; ¨Protocole; ADN; PCR.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00165/27663/25838.pdf

Experimental transmission of Marteilia refringens with special consideration of its life cycle
Provedor de dados: 5
Autores: Berthe, Franck; Pernas, Maria; Zerabib, Maamar; Haffner, Philippe; Thebault, Anne; Figueras, Antonio.
Ever since its first description the paramyxean parasite Marteilia refringens has been recognised as one of the most significant pathogens of bivalve molluscs. The existence of a complex life cycle has been postulated by many authors. However, the transmission route of M. refringens and its life cycle remain unresolved. Experimental observations are presented here that may help lead to a satisfactory hypothesis of the M. refringens life cycle. Trials were performed to test the infectivity of purified early and late stages of M. refringens by different routes of inoculation. Horizontal transmission was also tested by cohabitation with carriers in experimental tanks. Field cohabitation and exposure to natural sources of the parasite helped examine the role...
Tipo: Text
Palavras-chave: Marteilia refringens; Ostrea edulis; Transmission trial.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/1998/publication-2742.pdf

First description of French V. tubiashii strains pathogenic to mollusk: I. Characterization of isolates and detection during mortality events
Provedor de dados: 5
Autores: Travers, Marie-agnes; Mersni Achour, Rachida; Haffner, Philippe; Tourbiez, Delphine; Cassone, Anne-laure; Morga, Benjamin; Doghri, Ibtissem; Garcia, Celine; Renault, Tristan; Fruitier-arnaudin, Ingrid; Saulnier, Denis.
Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was...
Tipo: Text
Palavras-chave: Crassostrea gigas Pathogenicity Extracellular products Polyphasic approach; Real time PCR.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00189/30028/28743.pdf

Antiviral immunity in the Pacific oyster, Crassostrea gigas: last development and perspective
Provedor de dados: 5
Autores: Renault, Tristan; Faury, Nicole; Barbosa, Valerie; Moreau, Kevin; Haffner, Philippe; Pepin, Jean-francois.
Herpes-like viruses have been reported last decades in various marine mollusc species in association with mortality outbreaks throughout the world (Renault &amp; Novoa 2004). One of these viruses isolated during French outbreaks has been characterised as an unassigned member of the Herpesviridae family and named ostreid herpes virus 1 (OsHV-1) or Pacific oyster herpes virus (Davison et al. 2005). Studying anti-viral non-specific defence mechanisms (innate immunity) is of great interest, because they constitute the only one in molluscs. Therefore, innate immunity has be investigated in the Pacific cupped oyster, Crassostrea gigas. The main aim of the work was focused on the identification and the characterisation of genes induced by OsHV-1 in the Pacific...
Tipo: Text
Palavras-chave: Pathoogy; Protein gene; Immunity; Herpesviridae; OsHV 1; Crassostrea gigas; Pacific oyster.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4035.pdf

First evidence of a potential antibacterial activity involving a laccase-type enzyme of the phenoloxidase system in Pacific oyster Crassostrea gigas haemocytes
Provedor de dados: 5
Autores: Luna-acosta, Andrea; Saulnier, Denis; Pommier, Mylene; Haffner, Philippe; De Decker, Sophie; Renault, Tristan; Thomas-guyon, Helene.
Phenoloxidases (POs) are a group of copper proteins including tyrosinase, catecholase and laccase. In several insects and crustaceans, antibacterial substances are produced through the PO cascade, participating in the direct killing of invading microorganisms. However, although POs are widely recognised as an integral part of the invertebrate immune defence system, experimental evidence is lacking that these properties are conserved in molluscs, and more particularly in the Pacific oyster Crassostrea gigas. In the present study, Vibrio splendidus LGP32 and Vibrio aestuarianus 02/041 growths were affected, after being treated with C. gigas haemocyte lysate supernatant (HLS), and either a common substrate of POs, l-3,4-dihydroxyphenylalanine (l-DOPA), to...
Tipo: Text
Palavras-chave: Phenoloxidases; Antibacterial activity; Vibrio; Bivalve; Immunity.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00045/15598/13001.pdf

A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity
Provedor de dados: 5
Autores: Saulnier, Denis; De Decker, Sophie; Haffner, Philippe; Cobret, Laetitia; Robert, Maeva; Garcia, Celine.
A 4-year bacteriological survey (2003-2007) of four molluscs cultivated in France and faced with mortality episodes was performed by the French shellfish pathology network. The more abundant bacteria isolated during 92 mortality episodes, occurring mainly in Pacific oyster Crassostrea gigas, were identified by genotyping methods. It allowed us both to confirm the representativeness of Vibrio splendidus and Vibrio aestuarianus bacterial strains and to identify both a large number of Vibrio harveyi-related strains mainly detected during 2007 oyster mortality outbreaks and to a lesser extent bacterial strains identified as Shewanella colwelliana. Because metalloprotease has been reported to constitute a virulence factor in a few Vibrio strains pathogenic for...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/2009/publication-6974.pdf

Suivi bactérien d'un élevage larvaire de crevettes péneïdes - Ecloserie CAMCAR, 12 janvier - 12 février 1991
Provedor de dados: 5
Autores: Haffner, Philippe; Berthe, Franck.
La mission effectuée de mi-janvier à mi-février à l'écloserie CAMCAR (Colombie), avait pour objectif l'analyse quantitative et qualitative de la flore bactérienne présente en élevage larvaire de crevettes pénéides. L'intérêt principal du choix de ce site est de travailler dans une écloserie jeune (- de de deux ans) et n'ayant jamais rencontré de problème pathologique. Cette analyse fait donc le point sur les équilibres bactériens présents dans une écloserie industrielle saine mais bien évidemment dans un lieu et à une époque précise.
Tipo: Text
Palavras-chave: Ecloserie; Bactériologie; Flore bactérienne; Colombie.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00131/24192/22186.pdf