Consulta: repository:"56"
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Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury
Provedor de dados: 56
Autores: Zhang,Weiwei; Li,Ying; Wang,Peng.
Long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of cardiovascular diseases, especially in myocardial infarction and ischemia/reperfusion (I/R). However, the underlying molecular mechanism remains unclear. In this study, we determined the role and the possible underlying molecular mechanism of lncRNA-ROR in myocardial I/R injury. H9c2 cells and human cardiomyocytes (HCM) were subjected to either hypoxia/reoxygenation (H/R), I/R or normal conditions (normoxia). The expression levels of lncRNA-ROR were detected in serum of myocardial I/R injury patients, H9c2 cells, and HCM by qRT-PCR. Then, levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were measured by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA; Ischemia/reperfusion (I/R); Hypoxia/reoxygenation (H/R); Cell viability; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600609

Hypogammaglobulinemia: a diagnosis that must not be overlooked
Provedor de dados: 56
Autores: Pimenta,F.M.C.A.; Palma,S.M.U.; Constantino-Silva,R.N.; Grumach,A.S.
Humoral immunological defects are frequent and important causes of hypogammaglobulinemia, leading to recurrent infections, autoimmunity, allergies, and neoplasias. Usually, its onset occurs in childhood or during the second and third decades of life; however, the diagnosis is made, on average, 6 to 7 years afterwards. As a consequence, antibody defects can lead to sequelae. Here we describe the clinical-laboratory characteristics, treatment, and prognoses of patients with hypogammaglobulinemia. An observational, cross-sectional, and retrospective study of patients attending the recently established outpatient group of Clinical Immunology between 2013 and 2018 was carried out. Patients with IgG levels below 2 standard deviations from the mean values for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primary immunodeficiency; Common variable immunodeficiency; Hypogammaglobulinemia; Chemotherapy; Antibody defects; Immunoglobulin therapy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000608

Benzyl isothiocyanate inhibits invasion and induces apoptosis via reducing S100A4 expression and increases PUMA expression in oral squamous cell carcinoma cells
Provedor de dados: 56
Autores: Ma,Lei; Chen,Yongjun; Han,Rui; Wang,Shuangyi.
Benzyl isothiocyanate (BITC) has been shown to inhibit invasion and induce apoptosis of various types of cancer. However, its role on human oral squamous cell carcinoma (OSCC) cells is still not well elucidated. In the present study, we investigated the effect of BITC on apoptosis and invasion of SCC9 cells, and its underlying mechanisms in vitro and in vivo. SCC9 cells were exposed to BITC (5 and 25 μM) for 24 and 48 h. Cell growth, apoptosis, invasion, and migration were detected in vitro by MTT, FITC-conjugated annexin V/propidium iodide staining followed by flow cytometry, Matrigel-coated semi-permeable modified Boyden, and wound-healing assay. S100A4, PUMA, and MMP-9 expressions were detected to investigate its mechanisms. Xenotransplantation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral squamous cell carcinoma; Benzyl isothiocyanate; Apoptosis; S100A4; MMP-9; PUMA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400605

Are birth weight and maternal smoking during pregnancy associated with malnutrition and excess weight among school age children?
Provedor de dados: 56
Autores: Tomé,F.S.; Cardoso,V.C.; Barbieri,M.A.; Silva,A.A.M. da; Simões,V.M.F.; Garcia,C.A.; Bettiol,H.
In the late 1980's child malnutrition was still prevalent in Brazil, and child obesity was beginning to rise in the richest regions of the country. To assess the extent of the nutritional transition during the period and the influence of birth weight and maternal smoking on the nutritional condition of schoolchildren, we estimated the prevalence of excess weight and malnutrition in a cohort of Brazilian schoolchildren from 1987 to 1989. We calculated the body mass index (BMI) of 8- to 10-year-old schoolchildren born in Ribeirão Preto in 1978/79. We considered children with a BMI &lt;5th percentile (P5) to be malnourished, children with P5³BMI&lt;P85 to be thin and normal, and children with BMI ³P85 to be overweight. We evaluated the association of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Child malnutrition; Child obesity; Body mass index; Schoolchildren; Socioeconomic level.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900009

Delivery of the Sox9 gene promotes chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in an in vitro model
Provedor de dados: 56
Autores: Wang,Z.H.; Li,X.L.; He,X.J.; Wu,B.J.; Xu,M.; Chang,H.M.; Zhang,X.H.; Xing,Z.; Jing,X.H.; Kong,D.M.; Kou,X.H.; Yang,Y.Y.
SRY-related high-mobility-group box 9 (Sox9) gene is a cartilage-specific transcription factor that plays essential roles in chondrocyte differentiation and cartilage formation. The aim of this study was to investigate the feasibility of genetic delivery of Sox9 to enhance chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs). After they were isolated from human umbilical cord blood within 24 h after delivery of neonates, hUC-MSCs were untreated or transfected with a human Sox9-expressing plasmid or an empty vector. The cells were assessed for morphology and chondrogenic differentiation. The isolated cells with a fibroblast-like morphology in monolayer culture were positive for the MSC markers CD44, CD105,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic modification; Tissue engineering; Stem cells; Sox9; Chondrogenesis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400279

Mercury toxicity in the Amazon: contrast sensitivity and color discrimination of subjects exposed to mercury
Provedor de dados: 56
Autores: Rodrigues,A.R.; Souza,C.R.B.; Braga,A.M.; Rodrigues,P.S.S.; Silveira,A.T.; Damin,E.T.B.; Côrtes,M.I.T.; Castro,A.J.O.; Mello,G.A.; Vieira,J.L.F.; Pinheiro,M.C.N.; Ventura,D.F.; Silveira,L.C.L.
We measured visual performance in achromatic and chromatic spatial tasks of mercury-exposed subjects and compared the results with norms obtained from healthy individuals of similar age. Data were obtained for a group of 28 mercury-exposed subjects, comprising 20 Amazonian gold miners, 2 inhabitants of Amazonian riverside communities, and 6 laboratory technicians, who asked for medical care. Statistical norms were generated by testing healthy control subjects divided into three age groups. The performance of a substantial proportion of the mercury-exposed subjects was below the norms in all of these tasks. Eleven of 20 subjects (55%) performed below the norms in the achromatic contrast sensitivity task. The mercury-exposed subjects also had lower red-green...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury exposure; Mercury toxicity; Contrast sensitivity; Color vision; Visual dysfunction; Amazon riverside communities.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300018

Regulation of semaphorin 4D expression and cell proliferation of ovarian cancer by ERalpha and ERbeta
Provedor de dados: 56
Autores: Liu,Y.; Hou,Y.; Ma,L.; Sun,C.; Pan,J.; Yang,Y.; Zhou,H.; Zhang,J.
Ovarian cancer is one of the most common malignancies in women. Semaphorin 4D (sema 4D) is involved in the progress of multiple cancers. In the presence of estrogen-like ligands, estrogen receptors (ERα and ERβ) participate in the progress of breast and ovarian cancers by transcriptional regulation. The aim of the study was to investigate the role of sema 4D and elucidate the regulatory pattern of ERα and ERβ on sema 4D expression in ovarian cancers. Sema 4D levels were up-regulated in ovarian cancer SKOV-3 cells. Patients with malignant ovarian cancers had significantly higher sema 4D levels than controls, suggesting an oncogene role of sema 4D in ovarian cancer. ERα expressions were up-regulated in SKOV-3 cells compared with normal ovarian IOSE80...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semaphorin 4D expression; ERα; ERβ; Ovarian cancer; Cell proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300603

Disentangling the effects of insomnia and night work on cardiovascular diseases: a study in nursing professionals
Provedor de dados: 56
Autores: Silva-Costa,A.; Griep,R.H.; Rotenberg,L.
Cardiovascular diseases (CVDs) are known to be associated with poor sleep quality in general populations, but they have not been consistently associated with specific work schedules. Studies of CVD generally do not simultaneously consider sleep and work schedules, but that approach could help to disentangle their effects. We investigated the association between insomnia and a self-reported physician diagnosis of CVD in day and night workers, considering all sleep episodes during nocturnal and diurnal sleep. A cross-sectional study was conducted in 1307 female nursing professionals from 3 public hospitals, using baseline data from the “Health and Work in Nursing - a Cohort Study.” Participants were divided into two groups: i) day workers with no previous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular diseases; Insomnia; Night work; Nurse; Sleep.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200120

Dose-related effects of propericiazine in rats
Provedor de dados: 56
Autores: Cechin,E.M.; Quevedo,J.; Barichello,T.; Machado-Vieira,R.; Gentil,V.; Kapczinski,F.
We evaluated the effects of the neuroleptic agent propericiazine on animal models of anxiety and memory. Adult male Wistar rats (250 to 350 g) received intraperitoneal injections of propericiazine (0.05, 0.075 and 0.1 mg/kg), diazepam (1 mg/kg), saline, or diazepam vehicle (20% propylene glycol and 80% saline) 30 min prior to the experimental procedure. Animals (10-15 for each task) were tested for step-down inhibitory avoidance (0.3-mA footshock) and habituation to an open-field for memory assessment, and submitted to the elevated plus-maze to evaluate the effects of propericiazine in a model of anxiety. Animals treated with 0.075 mg/kg propericiazine showed a reduction in anxiety measures (P&lt;0.05) similar to that observed in those treated with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Propericiazine; Memory; Anxiety; Diazepam; Elevated plus-maze.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200010

Interleukin-12 as a biomarker of the beneficial effects of food restriction in mice receiving high fat diet or high carbohydrate diet
Provedor de dados: 56
Autores: de Almeida-Souza,C.B.; Antunes,M.M.; Godoy,G.; Schamber,C.R.; Silva,M.A.R.C.P.; Bazotte,R.B.
The impact of food restriction (FR) during 56 days on serum levels of cytokines in mice fed a high-fat diet (HFD) or high-carbohydrate diet (HCD) were evaluated. The amount of food was reduced 50% for HFD-FR and HCD-FR groups compared to mice receiving free access to HFD (HFD group) or HCD (HCD group). We quantified the serum levels of basic fibroblast growth factor, granulocyte-macrophage colony-stimulating factor, inducible protein 10, interferon γ, interleukin 1α (IL-1α), IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, keratinocyte chemoattractant, macrophage inflammatory protein-1α, monocyte chemotactic protein 1, monokine induced by IFN-γ, and tumor necrosis factor α. Only IL-12 levels were lower (P&lt;0.05), for both HFD-FR (HFD-FR vs HFD)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pro-inflammatory cytokines; Anti-inflammatory cytokines; Serum cytokines; Caloric restriction; Nutrition.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200606

The miR-17-92 cluster regulates FOG-2 expression and inhibits proliferation of mouse embryonic cardiomyocytes
Provedor de dados: 56
Autores: Xiang,Rui; Lei,Han; Chen,Mianzhi; Li,Qinwei; Sun,Huan; Ai,Jianzhong; Chen,Tielin; Wang,Honglian; Fang,Yin; Zhou,Qin.
MicroRNAs (miRNAs) have gradually been recognized as regulators of embryonic development; however, relatively few miRNAs have been identified that regulate cardiac development. A series of recent papers have established an essential role for the miRNA-17-92 (miR-17-92) cluster of miRNAs in the development of the heart. Previous research has shown that the Friend of Gata-2 (FOG-2) is critical for cardiac development. To investigate the possibility that the miR-17-92 cluster regulates FOG-2 expression and inhibits proliferation in mouse embryonic cardiomyocytes we initially used bioinformatics to analyze 3’ untranslated regions (3’UTR) of FOG-2 to predict the potential of miR-17-92 to target it. We used luciferase assays to demonstrate that miR-17-5p and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNAs; MiR-17-92; FOG-2; Proliferation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200007

HEV, TTV and GBV-C/HGV markers in patients with acute viral hepatitis
Provedor de dados: 56
Autores: Lyra,A.C.; Pinho,J.R.R.; Silva,L.K.; Sousa,L.; Saraceni,C.P.; Braga,E.L.; Pereira,J.E.; Zarife,M.A.S.; Reis,M.G.; Lyra,L.G.C.; Silva,L.C. da; Carrilho,F.J.
The aim of the present study was to evaluate the prevalence of HEV, TTV and GBV-C/GBV-C/HGV in patients with acute viral hepatitis A, B and non-A-C. We evaluated sera of 94 patients from a sentinel program who had acute hepatitis A (N = 40), B (N = 42) and non-A-C (N = 12); 71 blood donors served as controls. IgM and anti-HEV IgG antibodies were detected by enzyme immunoassay using commercial kits. TTV and GBV-C/HGV were detected by nested PCR; genotyping was done by sequencing and phylogenetic analysis. Anti-HEV IgG was present in 38, 10 and 17% of patients with hepatitis A, B and non-A-C. Four patients with hepatitis A and 1 with non-A-C hepatitis also had anti-HEV IgM detected in serum. TTV was detected in 21% of patients with acute hepatitis and in 31%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute hepatitis; Hepatitis E; TTV; GBV-C/HGV; Genotype; Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500015

Effects of nitric oxide on magnocellular neurons of the supraoptic nucleus involve multiple mechanisms
Provedor de dados: 56
Autores: Silva,M.P. da; Cedraz-Mercez,P.L.; Varanda,W.A.
Physiological evidence indicates that the supraoptic nucleus (SON) is an important region for integrating information related to homeostasis of body fluids. Located bilaterally to the optic chiasm, this nucleus is composed of magnocellular neurosecretory cells (MNCs) responsible for the synthesis and release of vasopressin and oxytocin to the neurohypophysis. At the cellular level, the control of vasopressin and oxytocin release is directly linked to the firing frequency of MNCs. In general, we can say that the excitability of these cells can be controlled via two distinct mechanisms: 1) the intrinsic membrane properties of the MNCs themselves and 2) synaptic input from circumventricular organs that contain osmosensitive neurons. It has also been...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnocellular neurons; Supraoptic nucleus; Vasopressin; Oxytocin; Nitric oxide; Homeostasis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200090

Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity
Provedor de dados: 56
Autores: Matsui,T.; Carneiro,C.R.W.; Leão,S.C.
The genome of Mycobacterium tuberculosis H37Rv contains three contiguous genes (plc-a, plc-b and plc-c) which are similar to the Pseudomonas aeruginosa phospholipase C (PLC) genes. Expression of mycobacterial PLC-a and PLC-b in E. coli and M. smegmatis has been reported, whereas expression of the native proteins in M. tuberculosis H37Rv has not been demonstrated. The objective of the present study was to demonstrate that native PLC-a is expressed in M. tuberculosis H37Rv. Sera from mice immunized with recombinant PLC-a expressed in E. coli were used in immunoblots to evaluate PLC-a expression. The immune serum recognized a 49-kDa protein in immunoblots against M. tuberculosis extracts. No bands were visible in M. tuberculosis culture supernatants or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Phospholipase C; Gene expression; Promoters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100003

Interleukin-27 augments the inhibitory effects of sorafenib on bladder cancer cells
Provedor de dados: 56
Autores: Cao,J.Y.; Yin,H.S.; Li,H.S.; Yu,X.Q.; Han,X.
Both sorafenib and interleukin-27 (IL-27) are antineoplastic drugs. This study aimed to investigate the synergistic effect of these two drugs on bladder cancer cells. HTB-9 and T24 cells were stimulated with IL-27 (50 ng/mL), sorafenib (2 μM) or the synergistic action of these two drugs. The cells without treatment acted as control. Cell proliferation, apoptosis and invasion were measured by bromodeoxyuridine assay, flow cytometry and modified Boyden chamber, respectively. Simultaneously, both modified Boyden chamber and scratch assay were used to assess cell migration. Finally, the phosphorylation levels of key kinases in the Akt/mechanistic target of rapamycin (mTOR)/mitogen-activated protein kinase (MAPK) pathway, and expression levels of matrix...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorafenib; Interleukin 27; Proliferation; Apoptosis; Invasion; Akt/mTOR/MAPK.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800610

[RETRACTED ARTICLE] Therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis
Provedor de dados: 56
Autores: Hai,Wang; Ping,Xu; Zhi-wen,Yang; Chun,Zhang.
Caspase recruitment domain-containing protein 9 (Card9) is located upstream of the nuclear factor kappa B (NF-κB) and p38 mitogen-activated protein kinase (MAPK) inflammatory pathways. This study investigated the therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis (SAP). SAP was induced by a retrograde infusion of 5.0% sodium taurocholate into the biliopancreatic duct of Sprague Dawley rats (n=54), which were then treated with pioglitazone. Blood and pancreatic tissues were harvested at 3, 6, and 12 h after SAP induction. Pancreatic pathological damage was evaluated by hematoxylin and eosin staining. Serum amylase, serum pro-inflammatory cytokines, and pancreatic myeloperoxidase (MPO) activities were determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caspase recruitment domain-containing protein 9; Pioglitazone; Severe acute pancreatitis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200612

Association of postalimentary lipemia with atherosclerotic manifestations
Provedor de dados: 56
Autores: Tentor,J.; Nakamura,R.T.; Gidlund,M.; Barros-Mazon,S.; Harada,L.M.; Zago,V.S.; Oba,J.F.; Faria,E.C. de.
We identified different lipemic and metabolic responses after the ingestion of a standardized meal by healthy adults and related them to atherosclerotic markers. Samples from 60 normolipidemic adults were collected before and after a liquid meal (40 g fat/m² body surface) at 0, 2, 4, 6, and 8 h for measurements of lipids, free fatty acids (FFA), insulin, cholesteryl ester transfer protein (CETP), autoantibodies to epitopes of oxidized LDL (oxLDL Ab), lipolytic activities, and apolipoprotein E polymorphism. Mean carotid intima-media thickness (cIMT) was determined by Doppler ultrasound. The volunteers were classified into early (N = 39) and late (N = 31) triacylglycerol (TAG) responders to the test meal. Late responders showed lower HDL cholesterol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Postalimentary lipemia; Autoantibodies to epitopes of oxidized LDL; Carotid intima-media thickness; Insulin; Free fatty acids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100014

Defensive responses to threat scenarios in Brazilians reproduce the pattern of Hawaiian Americans and non-human mammals
Provedor de dados: 56
Autores: Shuhama,R.; Del-Ben,C.M.; Loureiro,S.R.; Graeff,F.G.
A former study with scenarios conducted in Hawaii has suggested that humans share with non-human mammals the same basic defensive strategies - risk assessment, freezing, defensive threat, defensive attack, and flight. The selection of the most adaptive strategy is strongly influenced by features of the threat stimulus - magnitude, escapability, distance, ambiguity, and availability of a hiding place. Aiming at verifying if these strategies would be consistent in a different culture, 12 defensive scenarios were translated into Portuguese and adapted to the Brazilian culture. The sample consisted of male and female undergraduate students divided into two groups: 76 students, who evaluated the five dimensions of each scenario and 248 medical students, who...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Defensive strategies; Threat scenarios; Evolution-related responses; Lipp's Stress Symptoms Inventory for Adults.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400011

Baicalein restrains proliferation, migration, and invasion of human malignant melanoma cells by down-regulating colon cancer associated transcript-1
Provedor de dados: 56
Autores: Yang,Xiaoliang; Jiang,Jinjie; Zhang,Chunyan; Li,Yinghao.
Baicalein (BAI) is an acknowledged flavonoids compound, which is regarded as a useful therapeutic pharmaceutical for numerous cancers. However, its involvement in melanoma is largely unknown. This study aimed to examine the anti-melanoma function of BAI and unraveled the regulatory mechanism involved. A375 and SK-MEL-28 were treated with BAI for 24 h. Then, CCK-8 assay, flow cytometry, and transwell assay were carried out to investigate cell growth, migration, and invasion. RT-qPCR was applied to detect the expression of colon cancer associated transcript-1 (CCAT1) in melanoma tissues and cells. The functions of CCAT1 in melanoma cells were also evaluated. Western blot was utilized to appraise Wnt/β-catenin or MEK/ERK pathways. BAI restrained cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant melanoma; Colon cancer associated transcript-1; Wnt/β-catenin; MEK/ERK.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200602

Streptococcus mutans GlnK protein: an unusual PII family member
Provedor de dados: 56
Autores: Portugal,M.E.G.; Souza,E.M.; Pedrosa,F.O.; Benelli,E.M.
Streptococcus mutans is a Gram-positive bacterium present in the oral cavity, and is considered to be one of the leading causes of dental caries. S. mutans has a glnK gene, which codes for a PII-like protein that is possibly involved in the integration of carbon, nitrogen and energy metabolism in several organisms. To characterize the GlnK protein of S. mutans, the glnK gene was amplified by PCR, and cloned into the expression vectors pET29a(+) and pET28b(+). The native GlnK-Sm was purified by anion exchange (Q-Sepharose) and affinity (Hi-Trap Heparin) chromatography. The GlnK-His-Sm protein was purified using a Hi-Trap Chelating-Ni2+ column. The molecular mass of the GlnK-His-Sm proteins was 85 kDa as determined by gel filtration, indicating that this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GlnK; Streptococcus mutans; Nitrogen metabolism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500003

Effects of mercury on the arterial blood pressure of anesthetized rats
Provedor de dados: 56
Autores: Rossoni,L.V.; Amaral,S.M.C.; Vassallo,P.F.; França,A.; Oliveira,E.M.; Varner,K.J.; Mill,J.G.; Vassallo,D.V.
The available data suggests that hypotension caused by Hg2+ administration may be produced by a reduction of cardiac contractility or by cholinergic mechanisms. The hemodynamic effects of an intravenous injection of HgCl2 (5 mg/kg) were studied in anesthetized rats (N = 12) by monitoring left and right ventricular (LV and RV) systolic and diastolic pressures for 120 min. After HgCl2 administration the LV systolic pressure decreased only after 40 min (99 ± 3.3 to 85 ± 8.8 mmHg at 80 min). However, RV systolic pressure increased, initially slowly but faster after 30 min (25 ± 1.8 to 42 ± 1.6 mmHg at 80 min). Both right and left diastolic pressures increased after HgCl2 treatment, suggesting the development of diastolic ventricular dysfunction. Since HgCl2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Arterial blood pressure; Ventricular pressure; Pulmonary circulation; Diastolic dysfunction; Cholinergic activity.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800009

Stretch-induced nerve injury: a proposed technique for the study of nerve regeneration and evaluation of the influence of gabapentin on this model
Provedor de dados: 56
Autores: Machado,J.A.; Ghizoni,M.F.; Bertelli,J.; Teske,Gabriel C.; Teske,Guilherme C.; Martins,D.F.; Mazzardo-Martins,L.; Cargnin-Ferreira,E.; Santos,A.R.S.; Piovezan,A.P.
The rat models currently employed for studies of nerve regeneration present distinct disadvantages. We propose a new technique of stretch-induced nerve injury, used here to evaluate the influence of gabapentin (GBP) on nerve regeneration. Male Wistar rats (300 g; n=36) underwent surgery and exposure of the median nerve in the right forelimbs, either with or without nerve injury. The technique was performed using distal and proximal clamps separated by a distance of 2 cm and a sliding distance of 3 mm. The nerve was compressed and stretched for 5 s until the bands of Fontana disappeared. The animals were evaluated in relation to functional, biochemical and histological parameters. Stretching of the median nerve led to complete loss of motor function up to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nerve regeneration; Median nerve; Motor activity; Rat model.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100929

Effect of chronic fish oil supplementation on renal function of normal and cachectic rats
Provedor de dados: 56
Autores: Fernandez,R.; Piechnik,J.; Fabris,R.; Malnic,G.; Fernandes,L.C.
In the present study we determined the effect of chronic diet supplementation with n-3 PUFA on renal function of healthy and cachectic subjects by providing fish oil (1 g/kg body weight) to female rats throughout pregnancy and lactation and then to their offspring post-weaning and examined its effect on renal function parameters during their adulthood. The animals were divided into four groups of 5-10 rats in each group: control, control supplemented with fish oil (P), cachectic Walker 256 tumor-bearing (W), and W supplemented with fish oil (WP). Food intake was significantly lower in the W group compared to control (12.66 ± 4.24 vs 25.30 ± 1.07 g/day). Treatment with fish oil significantly reversed this reduction (22.70 ± 2.94 g/day). Tumor growth rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish oil supplementation; Renal function; Cachexia; Walker 256 tumor; Sodium excretion; Glomerular filtration rate.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000006

Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices
Provedor de dados: 56
Autores: Torres,I.L.S.; Gamaro,G.D.; Silveira-Cucco,S.N.; Michalowski,M.B.; Corrêa,J.B.; Perry,M.L.S; Dalmaz,C.
It has been suggested that glucocorticoids released during stress might impair neuronal function by decreasing glucose uptake by hippocampal neurons. Previous work has demonstrated that glucose uptake is reduced in hippocampal and cerebral cortex slices 24 h after exposure to acute stress, while no effect was observed after repeated stress. Here, we report the effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices and on plasma glucose and corticosterone levels. Male adult Wistar rats were exposed to restraint 1 h/day for 50 days in the chronic model. In the acute model there was a single exposure. Immediately or 24 h after stress, the animals were sacrificed and the hippocampus and cerebral...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Restraint stress; Chronic stress; CO2 production; Glucose oxidation; Hippocampus; Cerebral cortex.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100013

Early life, current socioeconomic position and serum lipids in young adulthood of participants in a cohort study initiated in 1978/1979
Provedor de dados: 56
Autores: Figueiredo,F.P.; Silva,A.A.M.; Bettiol,H.; Barbieri,M.A.; Batista,R.F.L.; Lamy Filho,F.; Silva,R.A.; Aragão,V.M.F.
The association between socioeconomic position (SEP) and serum lipids has been little studied and the results have been controversial. A total of 2063 young adults born in 1978/79 were evaluated at 23-25 years of age in the fourth follow-up of a cohort study carried out in Ribeirão Preto, SP, Brazil, corresponding to 31.8% of the original sample. Total serum cholesterol (TC), triglycerides, high-density cholesterol (HDL cholesterol) and low-density cholesterol (LDL cholesterol) were analyzed according to SEP at birth and during young adulthood. SEP was classified into tertiles of family income and a cumulative score of socioeconomic disadvantage was created. TC was 11.85 mg/100 mL lower among men of lower SEP in childhood (P &lt; 0.01) but no difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterol; HDL cholesterol; LDL cholesterol; Triglycerides; Cardiovascular diseases; Socioeconomic circumstances.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900014

Dynamic expression of desmin, α-SMA and TGF-β1 during hepatic fibrogenesis induced by selective bile duct ligation in young rats
Provedor de dados: 56
Autores: Gonçalves,J.O.; Tannuri,A.C.A.; Coelho,M.C.M.; Bendit,I.; Tannuri,U.
We previously described a selective bile duct ligation model to elucidate the process of hepatic fibrogenesis in children with biliary atresia or intrahepatic biliary stenosis. Using this model, we identified changes in the expression of alpha smooth muscle actin (α-SMA) both in the obstructed parenchyma and in the hepatic parenchyma adjacent to the obstruction. However, the expression profiles of desmin and TGF-β1, molecules known to be involved in hepatic fibrogenesis, were unchanged when analyzed by semiquantitative polymerase chain reaction (RT-PCR). Thus, the molecular mechanisms involved in the modulation of liver fibrosis in this experimental model are not fully understood. This study aimed to evaluate the molecular changes in an experimental model...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha smooth muscle actin; Desmin; Hepatic fibrogenesis; Selective bile duct ligation; RT-PCR; TGF-β1.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000850

Involvement of hippocampal AMPA glutamate receptor changes and the cAMP/protein kinase A/CREB-P signalling pathway in memory consolidation of an avoidance task in rats
Provedor de dados: 56
Autores: Bernabeu,R.; Cammarota,M.; Izquierdo,I.; Medina,J.H.
Training in step-down inhibitory avoidance (0.3-mA footshock) is followed by biochemical changes in rat hippocampus that strongly suggest an involvement of quantitative changes in glutamate AMPA receptors, followed by changes in the dopamine D1 receptor/cAMP/protein kinase A (PKA)/CREB-P signalling pathway in memory consolidation. AMPA binding to its receptor and levels of the AMPA receptor-specific subunit GluR1 increase in the hippocampus within the first 3 h after training (20-70%). Binding of the specific D1 receptor ligand, SCH23390, and cAMP levels increase within 3 or 6 h after training (30-100%). PKA activity and CREB-P levels show two peaks: a 35-40% increase 0 h after training, and a second increase 3-6 h later (35-60%). The results correlate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamate AMPA receptors; CAMP/PKA/CREB pathway; Memory formation; Rat hippocampus.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800008

Reduced hippocampal GABAergic function in Wistar audiogenic rats
Provedor de dados: 56
Autores: Drumond,L.E.; Kushmerick,C.; Guidine,P.A.M.; Doretto,M.C.; Moraes,M.F.D.; Massensini,A.R.
Epilepsy is a neurological disorder associated with excitatory and inhibitory imbalance within the underlying neural network. This study evaluated inhibitory γ-amino-butyric acid (GABA)ergic modulation in the CA1 region of the hippocampus of male Wistar rats and Wistar audiogenic rats (aged 90 ± 3 days), a strain of inbred animals susceptible to audiogenic seizures. Field excitatory postsynaptic potentials and population spike complexes in response to Schaffer collateral fiber stimulation were recorded in hippocampal slices before and during application of picrotoxin (50 µM, 60 min), a GABA A antagonist, and the size of the population spike was quantified by measuring its amplitude and slope. In control audiogenic-resistant Wistar rats (N = 9), picrotoxin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Extracellular recording; GABA; Hippocampus; Wistar audiogenic rats; Picrotoxin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000011

Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)-based scaffolds for tissue engineering
Provedor de dados: 56
Autores: Chang,H.M.; Wang,Z.H.; Luo,H.N.; Xu,M.; Ren,X.Y.; Zheng,G.X.; Wu,B.J.; Zhang,X.H.; Lu,X.Y.; Chen,F.; Jing,X.H.; Wang,L.
Development and selection of an ideal scaffold is of importance for tissue engineering. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a biocompatible bioresorbable copolymer that belongs to the polyhydroxyalkanoate family. Because of its good biocompatibility, PHBHHx has been widely used as a cell scaffold for tissue engineering. This review focuses on the utilization of PHBHHx-based scaffolds in tissue engineering. Advances in the preparation, modification, and application of PHBHHx scaffolds are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PHBHHx; Tissue engineering; Biomaterial.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700533

Peripheral markers of oxidative stress in chronic mercuric chloride intoxication
Provedor de dados: 56
Autores: Gutierrez,L.L.P.; Mazzotti,N.G.; Araújo,A.S.R.; Klipel,R.B.; Fernandes,T.R.G.; Llesuy,S.F.; Belló-Klein,A.
The present study was designed to evaluate the time course changes in peripheral markers of oxidative stress in a chronic HgCl2 intoxication model. Twenty male adult Wistar rats were treated subcutaneously daily for 30 days and divided into two groups of 10 animals each: Hg, which received HgCl2 (0.16 mg kg-1 day-1), and control, receiving the same volume of saline solution. Blood was collected at the first, second and fourth weeks of Hg administration to evaluate lipid peroxidation (LPO), total radical trapping antioxidant potential (TRAP), and superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and catalase (CAT). HgCl2 administration induced a rise (by 26%) in LPO compared to control (143 ± 10 cps/mg...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Heavy metals; Antioxidant enzymes; Lipid peroxidation; Total radical-trapping antioxidant potential; HgCl2.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600009

Infection with human papillomaviruses of sexual partners of women having cervical intraepithelial neoplasia
Provedor de dados: 56
Autores: Rombaldi,R.L.; Serafini,E.P.; Villa,L.L.; Vanni,A.C.; Baréa,F.; Frassini,R.; Xavier,M.; Paesi,S.
Epidemiological studies show that human papillomaviruses (HPV) are strongly related to cervical cancer and cervical intraepithelial neoplasias (CIN). Unlike the case for women, there are no consistent data on the natural history of HPV in the male population even though these viruses are prevalent in males. We carried out a prospective study to assess the prevalence of HPV in males as well as the factors that determine such infections in 99 male sexual partners of women with CIN. The genitalia of the males were physically examined and subjected to peniscopy for the collection of scrapings which were subjected to the polymerase chain reaction and restriction fragment length polymorphism to detect HPV. Of the 99 males sampled, 54 (54.5%) were positive for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical intraepithelial neoplasia; Epidemiology; Human papillomaviruses; Polymerase chain reaction.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200003

Clinical signs of pneumonia in children: association with and prediction of diagnosis by fuzzy sets theory
Provedor de dados: 56
Autores: Pereira,J.C.R.; Tonelli,P.A.; Barros,L.C.; Ortega,N.R.S.
The present study compares the performance of stochastic and fuzzy models for the analysis of the relationship between clinical signs and diagnosis. Data obtained for 153 children concerning diagnosis (pneumonia, other non-pneumonia diseases, absence of disease) and seven clinical signs were divided into two samples, one for analysis and other for validation. The former was used to derive relations by multi-discriminant analysis (MDA) and by fuzzy max-min compositions (fuzzy), and the latter was used to assess the predictions drawn from each type of relation. MDA and fuzzy were closely similar in terms of prediction, with correct allocation of 75.7 to 78.3% of patients in the validation sample, and displaying only a single instance of disagreement: a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiologic methods; Stochastic models; Fuzzy models; Clinical signs; Diagnosis; Data analysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500012

Identification of the main generator source of longitudinal muscle contraction in the earthworm ventral nerve cord
Provedor de dados: 56
Autores: Chang,Y.C.; Assmé,Z.; Caffaro,E.C.L.; Bartoszeck,A.B.
The main generator source of a longitudinal muscle contraction was identified as an M (mechanical-stimulus-sensitive) circuit composed of a presynaptic M-1 neuron and a postsynaptic M-2 neuron in the ventral nerve cord of the earthworm, Amynthas hawayanus, by simultaneous intracellular response recording and Lucifer Yellow-CH injection with two microelectrodes. Five-peaked responses were evoked in both neurons by a mechanical, but not by an electrical, stimulus to the mechanoreceptor in the shaft of a seta at the opposite side of an epidermis-muscle-nerve-cord preparation. This response was correlated to 84% of the amplitude, 73% of the rising rate and 81% of the duration of a longitudinal muscle contraction recorded by a mechano-electrical transducer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Earthworm muscle contraction; Main generator source; M-1 and M-2 neurons; Mechanical stimulus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000009

Long non-coding RNA CHCHD4P4 promotes epithelial-mesenchymal transition and inhibits cell proliferation in calcium oxalate-induced kidney damage
Provedor de dados: 56
Autores: Zhang,C.; Yuan,J.; Hu,H.; Chen,W.; Liu,M.; Zhang,J.; Sun,S.; Guo,Z.
Kidney stone disease is a major cause of chronic renal insufficiency. The role of long non-coding RNAs (lncRNAs) in calcium oxalate-induced kidney damage is unclear. Therefore, we aimed to explore the roles of lncRNAs in glyoxylate-exposed and healthy mouse kidneys using microarray technology and bioinformatics analyses. A total 376 mouse lncRNAs were differentially expressed between the two groups. Using BLAST, 15 lncRNA homologs, including AU015836 and CHCHD4P4, were identified in mice and humans. The AU015836 expression in mice exposed to glyoxylate and the CHCHD4P4 expression in human proximal tubular epithelial (HK-2) cells exposed to calcium oxalate monohydrate were analyzed, and both lncRNAs were found to be upregulated in response to calcium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney calculi; Long non-coding RNAs; Fibrosis; Cell proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100610

Cytoadhesion of Plasmodium falciparum-infected erythrocytes and the infected placenta: a two-way pathway
Provedor de dados: 56
Autores: Costa,F.T.M.; Avril,M.; Nogueira,P.A.; Gysin,J.
Malaria is undoubtedly the world's most devastating parasitic disease, affecting 300 to 500 million people every year. Some cases of Plasmodium falciparum infection progress to the deadly forms of the disease responsible for 1 to 3 million deaths annually. P. falciparum-infected erythrocytes adhere to host receptors in the deep microvasculature of several organs. The cytoadhesion of infected erythrocytes to placental syncytiotrophoblast receptors leads to pregnancy-associated malaria (PAM). This specific maternal-fetal syndrome causes maternal anemia, low birth weight and the death of 62,000 to 363,000 infants per year in sub-Saharan Africa, and thus has a poor outcome for both mother and fetus. However, PAM and non-PAM parasites have been shown to differ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasmodium falciparum; Cytoadhesion; Pregnancy-associated malaria; Var2CSA gene.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200003

Study of the embryofeto-toxicity of Crown-of-Thorns (Euphorbia milii) latex, a natural molluscicide
Provedor de dados: 56
Autores: Souza,C.A.M.; de-Carvalho,R.R.; Kuriyama,S.N.; Araujo,I.B.; Rodrigues,R.P.; Vollmer,R.S.; E.N.,Alves; Paumgartten,F.J.R.
The crude latex of Crown-of-Thorns (Euphorbia milii var. hislopii) is a potent plant molluscicide and a promising alternative to the synthetic molluscicides used in schistosomiasis control. The present study was undertaken to investigate the embryofeto-toxic potential of E. milii latex. The study is part of a comprehensive safety evaluation of this plant molluscicide. Lyophilized latex (0, 125, 250 and 500 mg/kg body weight) in corn oil was given by gavage to Wistar rats (N = 100) from days 6 to 15 of pregnancy and cesarean sections were performed on day 21 of pregnancy. The numbers of implantation sites, living and dead fetuses, resorptions and corpora lutea were recorded. Fetuses were weighed, examined for external malformations, and fixed for visceral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbiaceae; Embryofeto-toxicity; Plant molluscicides; Euphorbia milii; Reproductive toxicity; Safety evaluation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100011

Mortality rate after open Roux-in-Y gastric bypass: a 10-year follow-up
Provedor de dados: 56
Autores: Bruschi Kelles,S.M.; Diniz,M.F.H.S.; Machado,C.J.; Barreto,S.M.
The prevalence of obesity has increased to epidemic status worldwide. Thousands of morbidly obese individuals undergo bariatric surgery for sustained weight loss; however, mid- and long-term outcomes of this surgery are still uncertain. Our objective was to estimate the 10-year mortality rate, and determine risk factors associated with death in young morbidly obese adults who underwent bariatric surgery. All patients who underwent open Roux-in-Y gastric bypass surgery between 2001 and 2010, covered by an insurance company, were analyzed to determine possible associations between risk factors present at the time of surgery and deaths related and unrelated to the surgery. Among the 4344 patients included in the study, 79% were female with a median age of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Survival analysis; Mortality; Follow-up studies; Morbid obesity; Roux-in-Y gastric bypass; Surgeon expertise.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700617

Nonconventional amide bond formation catalysis: programming enzyme specificity with substrate mimetics
Provedor de dados: 56
Autores: Bordusa,F.
This article reports on the design and characteristics of substrate mimetics in protease-catalyzed reactions. Firstly, the basis of protease-catalyzed peptide synthesis and the general advantages of substrate mimetics over common acyl donor components are described. The binding behavior of these artificial substrates and the mechanism of catalysis are further discussed on the basis of hydrolysis, acyl transfer, protein-ligand docking, and molecular dynamics studies on the trypsin model. The general validity of the substrate mimetic concept is illustrated by the expansion of this strategy to trypsin-like, glutamic acid-specific, and hydrophobic amino acid-specific proteases. Finally, opportunities for the combination of the substrate mimetic strategy with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioorganic chemistry; Enzyme catalysis; Peptide synthesis; Proteases; Substrate mimetics.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500001

A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry
Provedor de dados: 56
Autores: Alves,L.P.S.; Almeida,A.T.; Cruz,L.M.; Pedrosa,F.O.; de Souza,E.M.; Chubatsu,L.S.; Müller-Santos,M.; Valdameri,G.
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flow cytometry; Nile red; Poly-3-hydroxybutyrate; Herbaspirillum seropedicae; Azospirillum brasilense.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100606

Daily blood pressure profile in Cushing's syndrome before and after surgery
Provedor de dados: 56
Autores: Kreze,A.; Mikulecky,M.
No significant difference has been demonstrated in the altered circadian blood pressure pattern between the pituitary-dependent and adrenal forms of Cushing's syndrome before surgery. The effect of therapy, however, proved to be different. The mesor was normalized in the pituitary-dependent Cushing's syndrome more conspicuously for systolic than for diastolic blood pressure. In Cushing's syndrome due to adrenal adenoma, systolic and diastolic blood pressure mesors have been even significantly "overnormalized" after treatment, being 11 to 27 and 2 to 13 mmHg (95% confidence) lower than corresponding mesors in controls. There was no difference between forms in the effect of treatment on blood pressure amplitudes, which remained significantly lower than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cushing's syndrome; Pituitary-dependent Cushing's syndrome; Cushing's syndrome due to adrenal adenoma; Blood pressure; Circadian rhythm; Surgery.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000004

Citations to papers from Brazilian institutions: a more effective indicator to assess productivity and the impact of research in graduate programs
Provedor de dados: 56
Autores: Meneghini,R.
A recent assessment of 4400 postgraduate courses in Brazil by CAPES (a federal government agency dedicated to the improvement of the quality of and research at the postgraduate level) stimulated a large amount of manifestations in the press, scientific journals and scientific congresses. This gigantic effort to classify 16,400 scientific journals in order to provide indicators for assessment proved to be puzzling and methodologically erroneous in terms of gauging the institutions from a metric point of view. A simple algorithm is proposed here to weigh the scientometric indicators that should be considered in the assessment of a scientific institution. I conclude here that the simple gauge of the total number of citations accounts for both the productivity...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Scientometrics; Institutional assessment; CAPES; Scientific journals; Citations.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800002

The husk fiber of Cocos nucifera L. (Palmae) is a source of anti-neoplastic activity
Provedor de dados: 56
Autores: Koschek,P.R.; Alviano,D.S.; Alviano,C.S.; Gattass,C.R.
In the present study, we investigated the in vitro anti-tumoral activities of fractions from aqueous extracts of the husk fiber of the typical A and common varieties of Cocos nucifera (Palmae). Cytotoxicity against leukemia cells was determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cells (2 x 104/well) were incubated with 0, 5, 50 or 500 µg/mL high- or low-molecular weight fractions for 48 h, treated with MTT and absorbance was measured with an ELISA reader. The results showed that both varieties have almost similar antitumoral activity against the leukemia cell line K562 (60.1 ± 8.5 and 47.5 ± 11.9% for the typical A and common varieties, respectively). Separation of the crude extracts with Amicon membranes...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cocos nucifera; Anti-tumor activity; Multidrug resistance; Leukemia cells.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000006

Photocytotoxicity of a 5-nitrofuran-ethenyl-quinoline antiseptic (Quinifuryl) to P388 mouse leukemia cells
Provedor de dados: 56
Autores: Daghastanli,N.A.; Degterev,I.A.; Tedesco,A.C.; Borissevitch,I.E.
Quinifuryl (MW 449.52), 2-(5'-nitro-2'-furanyl)ethenyl-4-{N-[4'-(N,N-diethylamino)-1'-methylbutyl]carbamoyl} quinoline, is a water soluble representative of a family of 5-nitrofuran-ethenyl-quinoline drugs which has been shown to be highly toxic to various lines of transformed cells in the dark. In the present study, the toxicity of Quinifuryl to P388 mouse leukemia cells was compared in the dark and under illumination with visible light (390-500 nm). Illumination of water solutions of Quinifuryl (at concentrations ranging from 0.09 to 9.0 µg/ml) in the presence of P388 cells resulted in its photodecomposition and was accompanied by elevated cytotoxicity. A significant capacity to kill P388 cells was detected at a drug concentration as low as 0.09 µg/ml....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phototoxicity; Nitrofurane derivatives; Quinifuryl; Oxygen and nitrogen active species.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200013

The cardiopulmonary reflexes of spontaneously hypertensive rats are normalized after regression of left ventricular hypertrophy and hypertension
Provedor de dados: 56
Autores: Uggere,T.A.; Abreu,G.R.; Sampaio,K.N.; Cabral,A.M.; Bissoli,N.S.
Cardiopulmonary reflexes are activated via changes in cardiac filling pressure (volume-sensitive reflex) and chemical stimulation (chemosensitive reflex). The sensitivity of the cardiopulmonary reflexes to these stimuli is impaired in the spontaneously hypertensive rat (SHR) and other models of hypertension and is thought to be associated with cardiac hypertrophy. The present study investigated whether the sensitivity of the cardiopulmonary reflexes in SHR is restored when cardiac hypertrophy and hypertension are reduced by enalapril treatment. Untreated SHR and WKY rats were fed a normal diet. Another groups of rats were treated with enalapril (10 mg kg-1 day-1, mixed in the diet; SHRE or WKYE) for one month. After treatment, the volume-sensitive reflex...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Volume-sensitive reflex; Cardiac hypertrophy; Chemosensitive reflex; SHR; Enalapril.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500014

L-histidine provokes a state-dependent memory retrieval deficit in mice re-exposed to the elevated plus-maze
Provedor de dados: 56
Autores: Serafim,K.R.; Kishi,M.; Canto-de-Souza,A.; Mattioli,R.
The effects of L-histidine (LH) on anxiety and memory retrieval were investigated in adult male Swiss Albino mice (weight 30-35 g) using the elevated plus-maze. The test was performed on two consecutive days: trial 1 (T1) and trial 2 (T2). In T1, mice received an intraperitoneal injection of saline (SAL) or LH before the test and were then injected again and retested 24 h later. LH had no effect on anxiety at the dose of 200 mg/kg since there was no difference between the SAL-SAL and LH-LH groups at T1 regarding open-arm entries (OAE) and open-arm time (OAT) (mean ± SEM; OAE: 4.0 ± 0.71, 4.80 ± 1.05; OAT: 40.55 ± 9.90, 51.55 ± 12.10, respectively; P &gt; 0.05, Kruskal-Wallis test), or at the dose of 500 mg/kg (OAE: 5.27 ± 0.73, 4.87 ± 0.66; OAT: 63.93 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-histidine; Anxiety; Emotional memory; Elevated plus-maze; Memory retrieval deficit.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100014

Neuroprotective effect of ketamine/xylazine on two rat models of Parkinson's disease
Provedor de dados: 56
Autores: Ferro,M.M.; Angelucci,M.E.M.; Anselmo-Franci,J.A.; Canteras,N.S.; Da Cunha,C.
There is a great concern in the literature for the development of neuroprotectant drugs to treat Parkinson's disease. Since anesthetic drugs have hyperpolarizing properties, they can possibly act as neuroprotectants. In the present study, we have investigated the neuroprotective effect of a mixture of ketamine (85 mg/kg) and xylazine (3 mg/kg) (K/X) on the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) or 6-hydroxydopamine (6-OHDA) rat models of Parkinson's disease. The bilateral infusion of MPTP (100 µg/side) or 6-OHDA (10 µg/side) into the substantia nigra pars compacta of adult male Wistar rats under thiopental anesthesia caused a modest (~67%) or severe (~91%) loss of tyrosine hydroxylase-immunostained cells, respectively. On the other hand, an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroprotection 6-Hydroxydopamine 1-Methyl-4-phenyl-1; 2; 3; 6- tetrahydropyridine Ketamine Parkinson's disease.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100012

Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
Provedor de dados: 56
Autores: Li,Lin; Zou,Changlin; Zhou,Zhenli; Wang,Ximo; Yu,Xiangyang.
The objective was to study the effect of mechanical intestinal obstruction in rats on the phenotype of interstitial cells of Cajal (ICC). Healthy Wistar rats were randomly divided into sham-operation group (C), one day obstruction group (M1), two days obstruction group (M2), and three days obstruction group (M3), with 10 rats in each group. The expression of SCF mRNA and c-Kit protein in intestinal tissue was investigated by RT-PCR and immunohistochemistry. Compared with the sham-operation group, the relative expression of SCF mRNA and the expression of c-Kit protein in intestinal tissue were significantly decreased in both obstruction groups. Levels decreased gradually with the prolongation of obstruction time, and significantly decreased on the 3rd day...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal obstruction; Interstitial cells of Cajal; Stem cell factor; C-Kit protein.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000611

Hyperhomocystinemia in patients with coronary artery disease
Provedor de dados: 56
Autores: Faria-Neto,J.R.; Chagas,A.C.P.; Bydlowski,S.P.; Lemos Neto,P.A.; Chamone,D.A.; Ramirez,J.A.F.; da Luz,P.L.
Hyperhomocystinemia has been related to an increased risk of cardiovascular disease in several studies. The C677T polymorphism for the gene that encodes the methylenetetrahydrofolate reductase enzyme (MTHFR) and low plasma folate levels are common causes of hyperhomocystinemia. Due to differences in nutritional patterns and genetic background among different countries, we evaluated the role of hyperhomocystinemia as a coronary artery disease (CAD) risk factor in a Brazilian population. The relation between homocysteine (Hcy) and the extent of CAD, measured by an angiographic score, was determined. A total of 236 patients referred for coronary angiography for clinical reasons were included. CAD was found in 148 (62.7%) patients and 88 subjects had normal or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperhomocystinemia; Homocysteine; Methylenetetrahydrofolate reductase; Atherosclerosis; Folic acid deficiency.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400005

Chlorophyll treatment combined with photostimulation increases glycolysis and decreases oxidative stress in the liver of type 1 diabetic rats
Provedor de dados: 56
Autores: Wunderlich,A.L.M.; Azevedo,S.C.S.F.; Yamada,L.A.; Bataglini,C.; Previate,C.; Campanholi,K.S.S.; Pereira,P.C.S.; Caetano,W.; Kaplum,V.; Nakamura,C.V.; Nakanishi,A.B.S.; Comar,J.F.; Pedrosa,M.M.D.; Godoi,V.A.F.
Photodynamic therapy (PDT) promotes cell death, and it has been successfully employed as a treatment resource for neuropathic complications of diabetes mellitus (T1DM) and hepatocellular carcinoma. The liver is the major organ involved in the regulation of energy homeostasis, and in pathological conditions such as T1DM, changes in liver metabolic pathways result in hyperglycemia, which is associated with multiple organic dysfunctions. In this context, it has been suggested that chlorophyll-a and its derivatives have anti-diabetic actions, such as reducing hyperglycemia, hyperinsulinemia, and hypertriglyceridemia, but these effects have not yet been proven. Thus, the biological action of PDT with chlorophyll-a on hepatic parameters related to energy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photopigment; Diabetes; Metabolism; Free Radicals; Phototherapy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100601

In vitro proliferation and differentiation of hepatic oval cells and their potential capacity for intrahepatic transplantation
Provedor de dados: 56
Autores: Li,Z.; Chen,J.; Li,L.; Ran,J.H.; Liu,J.; Gao,T.X.; Guo,B.Y.; Li,X.H.; Liu,Z.H.; Liu,G.J.; Gao,Y.C.; Zhang,X.L.
Hepatic oval cells (HOCs) are recognized as facultative liver progenitor cells that play a role in liver regeneration after acute liver injury. Here, we investigated the in vitro proliferation and differentiation characteristics of HOCs in order to explore their potential capacity for intrahepatic transplantation. Clusters or scattered HOCs were detected in the portal area and interlobular bile duct in the liver of rats subjected to the modified 2-acetylaminofluorene and partial hepatectomy method. Isolated HOCs were positive for c-kit and CD90 staining (99.8% and 88.8%, respectively), and negative for CD34 staining (3.6%) as shown by immunostaining and flow cytometric analysis. In addition, HOCs could be differentiated into hepatocytes and bile duct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic oval cells; Proliferation; Differentiation; Liver transplantation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800681

p53 as a prognostic factor in adrenocortical tumors of adults and children
Provedor de dados: 56
Autores: Sredni,S.T.; Zerbini,M.C.N.; Latorre,M.R.; Alves,V.A.F.
Mutations of the tumor suppressor gene p53 have been considered to be important determinants in several kinds of human cancer. Accumulation of p53 protein has been reported to correlate with more aggressive clinical behavior in some neoplasms. The role of p53 expression in adrenal cortical tumors (ACT) has not been elucidated but some studies have suggested its correlation with malignant behavior. Our objective was to determine if there is a correlation between the expression of immunoreactive p53 and the biological behavior of ACT. Fifty-seven ACT (21 from children and 36 from adults) were evaluated for p53 expression by immunohistochemistry in formalin-fixed paraffin-embedded tissue and analyzed in terms of outcome. The p53 parameter was utilized...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Adrenal cortical tumors; Histology; P53; Immunohistochemistry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100004

Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera
Provedor de dados: 56
Autores: Santana,A.N.C.; Trindade-Filho,E.M.; Cunha,R.B.; Sousa,M.V.; Cavalheiro,E.A.; Carvalho,K.M.
In this study, the behavioral and electroencephalographic (EEG) analysis of seizures induced by the intrahippocampal injection in rats of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera, was determined. The first alterations occurred during microinjection of granulitoxin (8 µg) into the dorsal hippocampus and consisted of seizure activity that began in the hippocampus and spread rapidly to the occipital cortex. This activity lasted 20-30 s, and during this period the rats presented immobility. During the first 40-50 min after its administration, three to four other similar short EEG seizure periods occurred and the rats presented the following behavioral alterations: akinesia, facial automatisms, head tremor, salivation,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bunodosoma granulifera; Granulitoxin; Sea anemones; Toxic peptides; Seizure; Electroencephalographic assay.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600016

Dexmedetomidine decreases the inflammatory response to myocardial surgery under mini-cardiopulmonary bypass
Provedor de dados: 56
Autores: Bulow,N.M.H.; Colpo,E.; Pereira,R.P.; Correa,E.F.M.; Waczuk,E.P.; Duarte,M.F.; Rocha,J.B.T.
Cardiopulmonary bypass (CPB) with extracorporeal circulation produces changes in the immune system accompanied by an increase in proinflammatory cytokines and a decrease in anti-inflammatory cytokines. We hypothesize that dexmedetomidine (DEX) as an anesthetic adjuvant modulates the inflammatory response after coronary artery bypass graft surgery with mini-CPB. In a prospective, randomized, blind study, 12 patients (4 females and 8 males, age range 42-72) were assigned to DEX group and compared with a conventional total intravenous anesthesia (TIVA) group of 11 patients (4 females and 7 males). The endpoints used to assess inflammatory and biochemical responses to mini-CPB were plasma interleukin (IL)-1, IL-6, IL-10, interferon (INF)-γ, tumor necrosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Systemic inflammatory response syndrome; Total intravenous anesthesia; Dexmedetomidine; Coronary artery bypass grafting surgery; Mini-cardiopulmonary bypass.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400702

Association between BDNF G196A (Val66Met) polymorphism and cognitive impairment in patients with Parkinson's disease: a meta-analysis
Provedor de dados: 56
Autores: Wang,Qian; Liu,Jianfang; Guo,Yikun; Dong,Guanzhong; Zou,Wenying; Chen,Zhuoyou.
Brain-derived neurotrophic factor (BDNF) is widely expressed in the central nervous system and prolongs the survival of dopaminergic neurons in the substantia nigra. Several studies have recently investigated the association between BDNF G196A (Val66Met), a single nucleotide polymorphism influencing cognitive processes, and cognitive impairment in Parkinson's disease (PD), but with contradictory findings. Thus, this meta-analysis was performed to clarify the possible association. Relevant studies were identified by a systematic search of PubMed, Embase, and China National Knowledge Infrastructure (CNKI) databases. The strength of the association was evaluated using crude odds ratios and 95% confidence interval. Finally, six studies involving 532 cases and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BDNF; Meta-analysis; Cognitive impairment; Parkinson's disease.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800604

Interrelationship between adipocytes and fibroblasts during acute damage to the subcutaneous adipose tissue of rats: an ultrastructural study
Provedor de dados: 56
Ultrastructural phenotypic transitional features were noted between adult adipocytes and fibroblasts in the subcutaneous adipose tissue of the dorsal pad of normal adult Wistar rats of both sexes, weighing 180-260 g, after acute injury either by the implantation of small (1.8 x 1 x 0.4 cm) perforated plastic boxes or by local heat application. Soon after the inflicted damage, fat-containing cells presented variable shapes. Early after damage, some of these cells were round, adipocyte-like, with numerous and large cytoplasmic fat droplets. A few days later, fat-containing cells became elongated, with the fat droplets in their cytoplasm becoming smaller and less numerous. The cells also showed a prominent active rough endoplasmic reticulum and newly formed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipocytes; Adipocyte potential for differentiation; Adipose tissue damage.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500009

Role of angiotensin II and vasopressin receptors within the supraoptic nucleus in water and sodium intake induced by the injection of angiotensin II into the medial septal area
Provedor de dados: 56
Autores: Antunes,V.R.; Camargo,G.M.P.A.; Saad,R.; Saad,W.A.; Luiz,A.C.; Camargo,L.A.A.
In this study we investigated the effects of the injection into the supraoptic nucleus (SON) of non-peptide AT1- and AT2-angiotensin II (ANG II) receptor antagonists, DuP753 and PD123319, as well as of the arginine-vasopressin (AVP) receptor antagonist d(CH2)5-Tyr(Me)-AVP, on water and 3% NaCl intake induced by the injection of ANG II into the medial septal area (MSA). The effects on water or 3% NaCl intake were assessed in 30-h water-deprived or in 20-h water-deprived furosemide-treated adult male rats, respectively. The drugs were injected in 0.5 µl over 30-60 s. Controls were injected with a similar volume of 0.15 M NaCl. Antagonists were injected at doses of 20, 80 and 180 nmol. Water and sodium intake was measured over a 2-h period. Previous...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin II; AT1 receptors; AT2 receptors; V1 receptors; Water intake; Sodium intake; Medial septal area; Supraoptic nucleus; Rats.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200013

Serum antigliadin antibody levels as a screening criterion before jejunal biopsy indication for celiac disease in a developing country
Provedor de dados: 56
Autores: Bahia,M.; Rabello,A.; Brasileiro Filho,G.; Penna,F.J.
The objective of the present study was to determine the efficacy of detection of antigliadin immunoglobulins G and A (IgG and IgA) for the diagnosis of celiac disease in a developing country, since other enteropathies might alter the levels of these antibodies. Three groups were studied: 22 patients with celiac disease (mean age: 30.6 months), 61 patients with other enteropathies (mean age: 43.3 months), and 46 patients without enteropathies (mean age: 96.9 months). Antigliadin IgG and IgA ELISA showed sensitivity of 90.9 and 95.5%, respectively. With the hypothetical values of prevalence ranging from 1:500 to 1:2000 liveborns, the positive predictive value varied from 8.5 to 2.3% for IgG and from 4.8 to 1.1% for IgA. Considering the patients without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celiac disease; Enteropathies; Antigliadin antibodies.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100007

Correlation between serum E-selectin levels and panoramic nailfold capillaroscopy in systemic sclerosis
Provedor de dados: 56
Autores: Valim,V.; Assis,L.S.S.; Simões,M.F.J.; Trevisani,V.F.M.; Pucinelli,M.L.C.; Andrade,L.E.C.
E-selectin is expressed by the activated endothelium and its plasma levels are increased in patients with systemic sclerosis. Eighteen patients fulfilling the American Rheumatism Association criteria for systemic sclerosis, 15 females and 3 males, 42-70 years old, 9 with diffuse and 9 with limited forms, were sequentially recruited for this study. Serum E-selectin levels were determined by commercially available ELISA and their association with nailfold capillaroscopic abnormalities was investigated. Nailfold capillaries were analyzed by 16X magnification wide-field capillaroscopy. Two parameters on capillaroscopy were used to correlate to serum E-selectin: deletion and ectasia. Data were analyzed statistically by the Student t-test and Spearman...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic sclerosis; Nailfold capillaroscopy; E-selectin; Adhesion molecules.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900018

Effect of (-)-∆9-tetrahydrocannabinoid on the hepatic redox state of mice
Provedor de dados: 56
Autores: Pinto,C.E.; Moura,E.; Serrão,M.P.; Martins,M.J.; Vieira-Coelho,M.A.
(-)-∆9-Tetrahydrocannabinol (∆9-THC), a psychoactive component of marijuana, has been reported to induce oxidative damage in vivo and in vitro. In this study, we administered ∆9-THC to healthy C57BL/6J mice aged 15 weeks in order to determine its effect on hepatic redox state. Mice were divided into 3 groups: ∆9-THC (N = 10), treated with 10 mg/kg body weight ∆9-THC daily; VCtrl (N = 10), treated with vehicle [1:1:18, cremophor EL® (polyoxyl 35 castor oil)/ethanol/saline]; Ctrl (N = 10), treated with saline. Animals were injected ip twice a day with 5 mg/kg body weight for 10 days. Lipid peroxidation, protein carbonylation and DNA oxidation were used as biomarkers of oxidative stress. The endogenous antioxidant defenses analyzed were glutathione (GSH)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: (-)-∆9-Tetrahydrocannabinol; Oxidative stress; Liver; Cannabinoid receptors; Cremophor EL®.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400001

Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells
Provedor de dados: 56
Autores: Ribeiro-Dias,F.; Russo,M.; Nascimento,F.R.F.; Barbuto,J.A.M.; Timenetsky,J.; Jancar,S.
Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elicited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited macrophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Macrophages; Cytotoxicity; Mycoplasma; Tumor cells; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100010

Expression of beta 2 integrin (CD18) in embryonic mouse and chicken heart
Provedor de dados: 56
Autores: Oliveira,L.A.M.; Baker,R.K.; Klewer,S.E.; Kitten,G.T.
Integrins are heterodimeric receptors composed of α and β transmembrane subunits that mediate attachment of cells to the extracellular matrix and counter-ligands such as ICAM-1 on adjacent cells. β2 integrin (CD18) associates with four different α (CD11) subunits to form an integrin subfamily, which has been reported to be expressed exclusively on leukocytes. However, recent studies indicate that β2 integrin is also expressed by other types of cells. Since the gene for β2 integrin is located in the region of human chromosome 21 associated with congenital heart defects, we postulated that it may be expressed in the developing heart. Here, we show the results from several different techniques used to test this hypothesis. PCR analyses indicated that β2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta 2 integrin; CD18; Cardiac development; Epithelial-mesenchymal transformation; Down syndrome; Mouse and chicken cardiogenesis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100005

Plasma clearance and biodistribution of oxidatively modified 99mTc-ß-VLDL in rabbits
Provedor de dados: 56
The biodistribution and removal from plasma (measured as fractional clearance rate, FCR, per hour) of native and oxidatively modified 99mtechnetium-labeled ß-very low density lipoprotein (99mTc-ß-VLDL) were investigated in hypercholesterolemic (HC) and control (C) three-month old New Zealand rabbits. The intracellular accumulation of ß-VLDL labeled with 99mTc was studied in vitro in THP-1 cells and monocyte-derived macrophages isolated from rabbits. After intravenous injection into C rabbits, copper-oxidized ß-VLDL (99mTc-ox-ß-VLDL) was cleared from the circulation faster (0.362 ± 0.070/h) than native ß-VLDL (99mTc-nat-ß-VLDL, 0.241 ± 0.070/h). In contrast, the FCR of 99mTc-ox-ß-VLDL in HC rabbits was lower (0.100 ± 0.048/h) than that of 99mTc-nat-ß-VLDL...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SS-very low density lipoprotein; Copper-mediated oxidation; Biodistribution; Fractional clearance rate; Atherosclerosis; Hypercholesterolemia; Cholesterol feeding; Rabbit.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600002

Effects of acid-base imbalance on vascular reactivity
Provedor de dados: 56
Autores: Celotto,A.C.; Capellini,V.K.; Baldo,C.F.; Dalio,M.B.; Rodrigues,A.J.; Evora,P.R.B.
Acid-base homeostasis maintains systemic arterial pH within a narrow range. Whereas the normal range of pH for clinical laboratories is 7.35-7.45, in vivo pH is maintained within a much narrower range. In clinical and experimental settings, blood pH can vary in response to respiratory or renal impairment. This altered pH promotes changes in vascular smooth muscle tone with impact on circulation and blood pressure control. Changes in pH can be divided into those occurring in the extracellular space (pHo) and those occurring within the intracellular space (pHi), although, extracellular and intracellular compartments influence each other. Consistent with the multiple events involved in the changes in tone produced by altered pHo, including type of vascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid-base balance; Nitric oxide; Vascular reactivity; Endothelium; Alkalosis; Acidosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600001

Effect of melatonin on the functional recovery from experimental traumatic compression of the spinal cord
Provedor de dados: 56
Autores: Schiaveto-de-Souza,A.; da-Silva,C.A.; Defino,H.L.A.; Bel,E.A.Del.
Spinal cord injury is an extremely severe condition with no available effective therapies. We examined the effect of melatonin on traumatic compression of the spinal cord. Sixty male adult Wistar rats were divided into three groups: sham-operated animals and animals with 35 and 50% spinal cord compression with a polycarbonate rod spacer. Each group was divided into two subgroups, each receiving an injection of vehicle or melatonin (2.5 mg/kg, intraperitoneal) 5 min prior to and 1, 2, 3, and 4 h after injury. Functional recovery was monitored weekly by the open-field test, the Basso, Beattie and Bresnahan locomotor scale and the inclined plane test. Histological changes of the spinal cord were examined 35 days after injury. Motor scores were progressively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Melatonin; Functional recovery.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400348

Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species
Provedor de dados: 56
Autores: Tiwari,F.; Singh,D.K.
Adult Lymnaea acuminata (average length 20-22 mm) were collected locally from lakes and low-lying submerged fields from Gorakhpur. The chemoattraction studies were made in round glass aquaria measuring 30 cm in diameter and filled to a depth of 10 mm with 500 ml dechlorinated tap water. Each aquarium was divided into four concentric zones. At the starting time of the assay 10 snails were placed on the circumference of outermost zone 0. Snail attractant pellets (SAP) were added simultaneously in the center of central zone 3. SAP of different amino acids were prepared at concentrations of 10, 20, 50, 80 and 100 mM/2% agar solution and, subsequently, spread to a uniform thickness of 5 mm. After cooling, SAP were cut in small pieces of 5 mm in diameter....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snails; Lymnaea acuminata; Fasciola; Amino acids; Attraction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400016

Trachoma among the Yanomami Indians
Provedor de dados: 56
Autores: Paula,J.S.; Medina,N.H.; Cruz,A.A.V.
The Yanomami are one of the last primitive groups of Indians living in Brazil. They have almost no contact with other cultures. The epidemiology of eye disease among Yanomami is virtually unknown. For the first time, a trachoma survey was conducted among Yanomami Indians in the State of Amazonas near the Venezuelan border of the Brazilian rain forest. Ophthalmic examination was carried out on a total of 613 individuals (338 males and 275 females) from eight Yanomami villages along the Marauiá River located in the upper Rio Negro Basin. Age was classified into three categories (children, adults, and elderly) and trachoma was classified into five grades: follicular, inflammatory intense, cicatricial, trichiasis, and corneal opacity. Trachoma was endemic in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trachoma; Yanomami; Epidemiology.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000007

Influence of progesterone on GAD65 and GAD67 mRNA expression in the dorsolateral striatum and prefrontal cortex of female rats repeatedly treated with cocaine
Provedor de dados: 56
Autores: Souza,M.F.; Toniazo,V.M.; Frazzon,A.P.G.; Barros,H.M.T.
Female rats are intensely affected by cocaine, with estrogen probably playing an important role in this effect. Progesterone modulates the GABA system and attenuates the effects of cocaine; however, there is no information about its relevance in changing GABA synthesis pathways after cocaine administration to female rats. Our objective was to investigate the influence of progesterone on the effects of repeated cocaine administration on the isoenzymes of glutamic acid decarboxylase (GAD65 and GAD67) mRNA in brain areas involved in the addiction circuitry. Ovariectomized, intact and progesterone replacement-treated female rats received saline or cocaine (30 mg/kg, ip) acutely or repeatedly. GAD isoenzyme mRNA levels were determined in the dorsolateral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocaine; Progesterone; GAD mRNA; Dorsolateral striatum; Prefrontal cortex.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100011

Relationship between matrix metalloproteinases and the occurrence and development of ovarian cancer
Provedor de dados: 56
Autores: Zhang,Y.; Chen,Q.
Ovarian cancer is one of the most malignant genital cancers, with a high mortality rate. Many researchers have suggested that matrix metalloproteinases (MMPs) have remarkably high expression in ovarian cancer tissues. MMPs are considered to be related to the occurrence, development, invasion and metastasis of ovarian cancer. Moreover, some studies have discovered that the unbalance between MMPs and tissue inhibitor of metalloproteinases (TIMPs) are associated with the malignant phenotype of tumors. This review summarizes the latest research progress of MMPs in ovarian cancer. The investigation of MMP mechanism in ovarian cancer will facilitate the development of effective anti-tumor drugs, and thereby improve the survival rate of patients with ovarian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovarian cancer; Matrix metalloproteinases (MMPs); Tissue inhibitor of metalloproteinases (TIMPs); Invasion; Metastasis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600301

Maternal aggression in Wistar rats: effect of 5-HT2A/2C receptor agonist and antagonist microinjected into the dorsal periaqueductal gray matter and medial septum
Provedor de dados: 56
Autores: Almeida,R.M.M. de; Giovenardi,M.; Silva,S.P. da; Oliveira,V.P. de; Stein,D.J.
The objective of the present study was to assess the role of the 5-HT2A/2C receptor at two specific brain sites, i.e., the dorsal periaqueductal gray matter (DPAG) and the medial septal (MS) area, in maternal aggressive behavior after the microinjection of either a 5-HT2A/2C receptor agonist or antagonist. Female Wistar rats were microinjected on the 7th postpartum day with the selective agonist alpha-methyl-5-hydroxytryptamine maleate (5-HT2A/2C) or the antagonist 5-HT2A/2C, ketanserin. The agonist was injected into the DPAG at 0.2 (N = 9), 0.5 (N = 10), and 1.0 µg/0.2 µl (N = 9), and the antagonist was injected at 1.0 µg/0.2 µl (N = 9). The agonist was injected into the medial septal area (MS) at 0.2 (N = 9), 0.5 (N = 7), and 1.0 µg/0.2 µl (N = 6) and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: 5-HT receptors; Ketanserin; Alpha-Methyl-5-hydroxytrypta- mine maleate; Microinjection; Serotonin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400014

2-Methyl 2-butanol suppresses human retinoblastoma cells through cell cycle arrest and autophagy
Provedor de dados: 56
Autores: Li,Xiangyun; Zhu,Xiangxiang; Xu,Chong; Wu,Jianhua.
2-Methyl-2-butanol (MBT) is a chemical compound from the group of alcohols more specifically pentanols, which has shown an excellent anti-cancer activity in our previous study. However, its mechanism of action remains unclear. The present study was designed to investigate the anti-cancer effect of MBT on human retinoblastoma cells. The results showed that the use of MBT leads to HXO-RB44 cell death but is cytotoxic to normal cells at higher concentrations. It showed a dose- as well as a time-dependent inhibition of HXO-RB44 cells. P27 is a cell cycle inhibitory protein, which plays an important role in cell cycle regulation whereas cyclin-B1 is a regulatory protein involved in mitosis. MBT increased the cell cycle arrest in a dose-dependent manner by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-methyl-2-butanol; Retinoblastoma; Cell cycle arrest; Autophagy; Phosphoinositide 3-kinase (PI3K)/Akt.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500605

Topographic abnormality of slow cortical potentials in schizophrenia
Provedor de dados: 56
Autores: Basile,L.F.H.; Yacubian,J.; Ferreira,B.L.C.; Valim,A.C.; Gattaz,W.F.
A recent study from our laboratory has provided evidence for the generation of slow potentials occurring in anticipation to task-performance feedback stimuli, in multiple association cortical areas, consistently including two prefrontal areas. In the present study, we intended to determine whether these slow potentials would indicate some abnormality (topographic) in schizophrenic patients, and thus serve as an indication of abnormal association cortex activity. We recorded slow potentials while subjects performed a paired-associates memory task. A 123-channel EEG montage and common average reference were used for 20 unmedicated schizophrenic (mean duration of illness: 11.3 ± 9.2 years; mean number of previous hospitalizations: 1.2 ± 1.9) and 22 healthy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-resolution EEG; Slow potentials; Contingent negative variations; Schizophrenia; Source localization; Functional brain mapping.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100014

Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages
Provedor de dados: 56
Autores: dos Santos,D.P.; Muniz,I.P.R.; Queiroz,A.F.; Pereira,I.S.; Souza,M.P.A.; Lima,L.J.; Sousa,L.R.O.; Ribeiro,I.S.; Galantini,M.P.L.; Marques,L.M.; Figueiredo,T.B.; da Silva,R.A.A.
Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Staphylococcus aureus; Mice; Air pouch; Antibodies.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500616

Role of sympathetic nervous system and neuropeptides in obesity hypertension
Provedor de dados: 56
Autores: Hall,J.E.; Brands,M.W.; Hildebrandt,D.A.; Kuo,J.; Fitzgerald,S.
Obesity is the most common cause of human essential hypertension in most industrialized countries. Although the precise mechanisms of obesity hypertension are not fully understood, considerable evidence suggests that excess renal sodium reabsorption and a hypertensive shift of pressure natriuresis play a major role. Sympathetic activation appears to mediate at least part of the obesity-induced sodium retention and hypertension since adrenergic blockade or renal denervation markedly attenuates these changes. Recent observations suggest that leptin and its multiple interactions with neuropeptides in the hypothalamus may link excess weight gain with increased sympathetic activity. Leptin is produced mainly in adipocytes and is believed to regulate energy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Leptin; Angiotensin; Kidney; Sodium excretion.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600001

Molecular detection of Leishmania spp in Lutzomyia longipalpis in the city of Lavras, Minas Gerais, Brazil
Provedor de dados: 56
Autores: Castro,J.C.; Bueno,L.L.; Milagres,T.F.; Rêgo,F.D.; Gontijo,C.M.F.; Peconick,A.P.; Andrade,A.J.; Barçante,T.A.; Barçante,J.M.P.
Leishmaniasis is a neglected disease that affects a large part of the world population. Knowing the sand fly fauna of a region is of fundamental importance for guiding health surveillance actions related to the prevention and control of leishmaniasis. A total of 86 specimens of sand flies (60 females and 26 males) were collected. Using the classification proposed by Galati (2003), the following species were identified: Lutzomyia longipalpis (Lutz &amp; Neiva, 1912), Migonemyia migonei (França, 1920), Evandromyia cortelezzi (Brethes, 1923), Ev. sallesi (Galvão &amp; Coutinho, 1939), Nyssomyia whitmani (Atunes &amp; Coutinho, 1939), Psathyromyia lutziana (Costa Lima, 1932), Ev. lenti (Mangabeira, 1938), Brumptomyia sp. (França and Parrot, 1921), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Sand fly; Epidemiology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900603

Preeclampsia: from epidemiological observations to molecular mechanisms
Provedor de dados: 56
Autores: López-Jaramillo,P.; Casas,J.P.; Serrano,N.
Preeclampsia is the main cause of maternal mortality and is associated with a five-fold increase in perinatal mortality in developing countries. In spite of this, the etiology of preeclampsia is unknown. The present article analyzes the contradictory results of the use of calcium supplementation in the prevention of preeclampsia, and tries to give an explanation of these results. The proposal of an integrative model to explain the clinical manifestations of preeclampsia is discussed. In this proposal we suggest that preeclampsia is caused by nutritional, environmental and genetic factors that lead to the creation of an imbalance between the free radicals nitric oxide, superoxide and peroxynitrate in the vascular endothelium. The adequate interpretation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Calcium supplementation; Nitric oxide; Infection; Inflammation; Oxidative stress; Endothelial dysfunction.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000001

Activation of locus coeruleus heme oxygenase-carbon monoxide pathway promoted an anxiolytic-like effect in rats
Provedor de dados: 56
Autores: Carvalho-Costa,P.G.; Branco,L.G.S.; Leite-Panissi,C.R.A.
The heme oxygenase-carbon monoxide pathway has been shown to play an important role in many physiological processes and is capable of altering nociception modulation in the nervous system by stimulating soluble guanylate cyclase (sGC). In the central nervous system, the locus coeruleus (LC) is known to be a region that expresses the heme oxygenase enzyme (HO), which catalyzes the metabolism of heme to carbon monoxide (CO). Additionally, several lines of evidence have suggested that the LC can be involved in the modulation of emotional states such as fear and anxiety. The purpose of this investigation was to evaluate the activation of the heme oxygenase-carbon monoxide pathway in the LC in the modulation of anxiety by using the elevated plus maze test (EPM)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heme oxygenase Carbon monoxide Locus coeruleus Anxiety Elevated plus maze; Light/dark box.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500601

Mapping cancer, cardiovascular and malaria research in Brazil
Provedor de dados: 56
Autores: Rodrigues,P.S.; Fonseca,L.; Chaimovich,H.
This paper presents performance indicators for the Brazilian cancer, cardiovascular and malaria research areas from 1981 to 1995. The data show an increasing number of papers since 1981 and author numbers indicate a continuous growth of the scientific community and suggest an expected impact of scientific activity on biomedical education. The data also characterize cardiovascular research as a well-established area and cancer research as a faster growing consolidating field. The 1989-1994 share of Brazilian articles among world publications shows a growing trend for the cancer (1.61) and cardiovascular (1.59) areas, and a decrease for the malaria area (0.89). The burden of the three diseases on society is contrasted by the small number of consolidated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Cardiovascular research; Malaria; Bibliometric indicators; Health indicators.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800001

Diclofenac plasma protein binding: PK-PD modelling in cardiac patients submitted to cardiopulmonary bypass
Provedor de dados: 56
Twenty-four surgical patients of both sexes without cardiac, hepatic, renal or endocrine dysfunctions were divided into two groups: 10 cardiac surgical patients submitted to myocardial revascularization and cardiopulmonary bypass (CPB), 3 females and 7 males aged 65 ± 11 years, 74 ± 16 kg body weight, 166 ± 9 cm height and 1.80 ± 0.21 m2 body surface area (BSA), and control, 14 surgical patients not submitted to CPB, 11 female and 3 males aged 41 ± 14 years, 66 ± 14 kg body weight, 159 ± 9 cm height and 1.65 ± 0.16 m2 BSA (mean ± SD). Sodium diclofenac (1 mg/kg, im Voltaren 75® twice a day) was administered to patients in the Recovery Unit 48 h after surgery. Venous blood samples were collected during a period of 0-12 h and analgesia was measured by the...
Palavras-chave: Diclofenac; Plasma protein binding; Cardiopulmonary bypass; Kinetic-dynamic parameters; Analgesia; Visual analogue scale.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300010

Experimental chronic entrapment of the sciatic nerve in adult hamsters: an ultrastructural and morphometric study
Provedor de dados: 56
Autores: Prinz,R.A.D.; Nakamura-Pereira,M.; De-Ary-Pires,B.; Fernandes,D.S.; Fabião-Gomes,B.D.S.V.; Bunn,P.S.; Martinez,A.M.B.; Pires-Neto,M.A.; Ary-Pires,R.
Entrapment neuropathy is a group of clinical disorders involving compression of a peripheral nerve and interference with nerve function mostly through traction injury. We have investigated the chronic compression of peripheral nerves as an experimental procedure for detecting changes in ultrastructural nerve morphology. Adult hamsters (Mesocricetus auratus, N = 30) were anesthetized with a 25% pentobarbital solution and received a cuff around the right sciatic nerve. Left sciatic nerves were not operated (control group). Animals survived for varying times (up to 15 weeks), after which they were sacrificed and both sciatic nerves were immediately fixed with a paraformaldehyde solution. Experimental nerves were divided into segments based upon their distance...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nerve entrapment; Ultrastructure; Morphometry; Hamster; Renaut body.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900015

Absence-like seizures in adult rats following pilocarpine-induced status epilepticus early in life
Provedor de dados: 56
Autores: Ferreira,B.L.C.; Valle,A.C.; Cavalheiro,E.A.; Timo-Iaria,C.
Administration of pilocarpine causes epilepsy in rats if status epilepticus (SE) is induced at an early age. To determine in detail the electrophysiological patterns of the epileptogenic activity in these animals, 46 Wistar rats, 7-17 days old, were subjected to SE induced by pilocarpine and electro-oscillograms from the cortex, hippocampus, amygdala, thalamus and hypothalamus, as well as head, rostrum and vibrissa, eye, ear and forelimb movements, were recorded 120 days later. Six control animals of the same age range did not show any signs of epilepsy. In all the rats subjected to SE, iterative spike-wave complexes (8.1 ± 0.5 Hz in frequency, 18.9 ± 9.1 s in duration) were recorded from the frontal cortex during absence fits. However, similar spike-wave...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Absence seizures; Spike-wave; Status epilepticus; Pilocarpine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200010

Survival benefits of interferon-based therapy in patients with recurrent hepatitis C after orthotopic liver transplantation
Provedor de dados: 56
Autores: Zanaga,L.P.; Vigani,A.G.; Angerami,R.N.; Giorgetti,A.; Escanhoela,C.A.F.; Ataíde,E.C.; Boin,I.F.S.F.; Stucchi,R.S.B.
Recurrent hepatitis C after orthotopic liver transplantation (OLT) is universal and can lead to graft failure and, consequently, reduced survival. Hepatitis C treatment can be used to prevent these detrimental outcomes. The aim of this study was to describe rates of hepatitis C recurrence and sustained virological response (SVR) to interferon-based treatment after OLT and its relationship to survival and progression of liver disease through retrospective analysis of medical records of 127 patients who underwent OLT due to cirrhosis or hepatocellular carcinoma secondary to chronic hepatitis C between January 2002 and December 2013. Fifty-six patients were diagnosed with recurrent disease, 42 started interferon-based therapy and 37 completed treatment....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Liver transplantation; Sustained virological response; Recurrent hepatitis C; Transplantation outcomes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100701

Beneficial effects of exercise training (treadmill) on insulin resistance and nonalcoholic fatty liver disease in high-fat fed C57BL/6 mice
Provedor de dados: 56
Autores: Marques,C.M.M.; Motta,V.F.; Torres,T.S.; Aguila,M.B.; Mandarim-de-Lacerda,C.A.
C57BL/6 mice develop signs and symptoms comparable, in part, to the human metabolic syndrome. The objective of the present study was to evaluate the effects of exercise training on carbohydrate metabolism, lipid profile, visceral adiposity, pancreatic islet alterations, and nonalcoholic fatty liver disease in C57BL/6 mice. Animals were fed one of two diets during an 8-week period: standard (SC, N = 12) or very high-fat (HF, N = 24) chow. An exercise training protocol (treadmill) was then established and mice were divided into SC and HF sedentary (SC-Sed, HF-Sed), exercised groups (SC-Ex, HF-Ex), or switched from HF to SC (HF/SC-Sed and HF/SC-Ex). HF/HF-Sed mice had the greatest body mass (65% more than SC/SC-Sed; P &lt; 0.0001), and exercise reduced it by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Lipid metabolism; Metabolic syndrome; Aerobic training; Fatty liver; Stereology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500008

Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
Provedor de dados: 56
Autores: Prezoto,B.C.; Couto,G.K.; Rossoni,L.V.; Schoorlemmer,G.H.M.; Carillo,B.A.; Campos,R.R.
The generation of bradykinin (BK; Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) in blood and kallidin (Lys-BK) in tissues by the action of the kallikrein-kinin system has received little attention in non-mammalian vertebrates. In mammals, kallidin can be generated by the coronary endothelium and myocytes in response to ischemia, mediating cardioprotective events. The plasma of birds lacks two key components of the kallikrein-kinin system: the low molecular weight kininogen and a prekallikrein activator analogous to mammalian factor XII, but treatment with bovine plasma kallikrein generates ornitho-kinin [Thr6,Leu8]-BK. The possible cardioprotective effect of ornitho-kinin infusion was investigated in an anesthetized, open-chest chicken model of acute coronary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Infarct size; Kallikrein-kinin system; Ornitho-kinin; Factor XII.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900009

Effect of different durations of overdistention on rat bladder function and morphology
Provedor de dados: 56
Autores: Jin,Xiao-Dong; Cao,Min; Zhou,Xie-Lai; Yin,Hong-Ping; Chen,Zhao-Dian; Xu,Ning; Jiang,Hai.
We investigated the contribution of the duration of overdistention (DOD) to rat bladder function and morphology and explored its possible molecular mechanisms. Bladder overdistention was induced in male Sprague-Dawley rats (200-250 g) by an infusion of saline. Forty rats were divided into 5 groups submitted to different DOD, i.e., 1, 2, 4, and 8 h, and control. Bladder function was evaluated by cystometry. Morphological changes were observed by light and transmission electron microscopy. Compared to control (44.567 ± 3.472 cmH2O), the maximum detrusor pressure of groups with 2-, 4- and 8-h DOD decreased significantly (means ± SEM): 32.774 ± 3.726, 31.321 ± 2.847, and 29.238 ± 3.724 cmH2O. With the increase of DOD, inflammatory infiltration and impairment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute urinary retention; Bladder overdistention; Urodynamics; Ultrastructure; Apoptosis; Rat.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000006

Functional and morphological effects of resistance exercise on disuse-induced skeletal muscle atrophy
Provedor de dados: 56
Autores: Nicastro,H.; Zanchi,N.E.; da Luz,C.R.; Lancha Jr.,A.H.
Abstract The reduction of skeletal muscle loss in pathological states, such as muscle disuse, has considerable effects in terms of rehabilitation and quality of life. Since there is no currently effective and safe treatment available for skeletal muscle atrophy, the search for new alternatives is necessary. Resistance exercise (RE) seems to be an important tool in the treatment of disuse-induced skeletal muscle atrophy by promoting positive functional (strength and power) and structural (hypertrophy and phenotypic changes) adaptive responses. Human and animal studies using different types of resistance exercise (flywheel, vascular occlusion, dynamic, isometric, and eccentric) have obtained results of great importance. However, since RE is a complex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resistance exercise; Vascular occlusion; Eccentric exercise; Disuse; Atrophy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100001

Sleep disorder or simple sleep ontogeny? Tendency for morningness is associated with worse sleep quality in the elderly
Provedor de dados: 56
Autores: Barbosa,A.A.; Miguel,M.A.L.; Tufik,S.; Sabino,F.C.; Cendoroglo,M.S.; Pedrazzoli,M.
The objective of this study was to evaluate the alterations in sleep and circadian parameters during the aging process. The study sample comprises volunteers older than 18 up to 90 years of age that answered the Pittsburgh Sleep Quality Index (PSQI) and the Horne and Östberg circadian preference questionnaire. We observed that the shift to morningness with increasing age is associated with a significant worsening in sleep quality. We discuss that this sleep profile characterized by morningness and worse sleep quality observed in elderly, when compared to younger people, reflects not necessarily a pathological state, but an expected profile for this age group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly; Sleep quality; Circadian rhythmicity; Chronotype; Sleep ontogeny.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000606

Angiotensin and baroreflex control of the circulation
Provedor de dados: 56
Autores: Head,G.A.; Saigusa,T.; Mayorov,D.N.
There is a close association between the location of angiotensin (Ang) receptors and many important brain nuclei involved in the regulation of the cardiovascular system. The present review encompasses the physiological role of Ang II in the brainstem, particularly in relation to its influence on baroreflex control of the heart and kidney. Activation of AT1 receptors in the brainstem by fourth ventricle (4V) administration to conscious rabbits or local administration of Ang II into the rostral ventrolateral medulla (RVLM) of anesthetized rabbits acutely increases renal sympathetic nerve activity (RSNA) and RSNA baroreflex responses. Administration of the Ang antagonist Sarile into the RVLM of anesthetized rabbits blocked the effects of Ang II on the RSNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; AT1 receptors; Losartan; Renal sympathetic activity; Baroreflex; Rostral ventrolateral medulla; Blood pressure; Rabbit.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900005

Influence of Treg cells and HBV genotype on sustained response and drug resistance in the treatment with nucleoside drugs
Provedor de dados: 56
Autores: Zhang,Y.R.; Li,B.; Wang,C.X.; Zhou,N.; Qi,W.; Li,X.L.; Wu,L.Y.; Wei,S.F.; Zhang,Y.D.
We aimed to investigate the influence of regulatory T cells including CD4+CD25+, CD8+CD28- and hepatitis B virus (HBV) genotype on sustained virological response and tolerance of nucleoside drugs. One hundred and thirty-seven patients were enrolled. Lamivudine was administered to 84 patients. Entecavir was administered to the other 53 patients. Before treatment, biochemical tests, HBV DNA load, HBV serum level, HBV genotype, PB CD3+, CD4+, CD8+, CD4+CD25+/CD3+, and CD8+CD28-/CD3+ frequencies were measured. Based on HBV DNA loads after 4 weeks of therapy, patients were divided into response group and suboptimal response group. The lamivudine group received treatment continuously, and then patients were categorized into non-resistance group and resistance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B chronic; CD4+CD25+; CD8+CD28-; Regulatory T cells; Viral response; Nucleoside.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300606

Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
Provedor de dados: 56
Autores: Sukoyan,M.A.; Kerkis,A.Y.; Mello,M.R.B.; Kerkis,I.E.; Visintin,J.A.; Pereira,L.V.
Embryonic stem cells are totipotent cells derived from the inner cell mass of blastocysts. Recently, the development of appropriate culture conditions for the differentiation of these cells into specific cell types has permitted their use as potential therapeutic agents for several diseases. In addition, manipulation of their genome in vitro allows the creation of animal models of human genetic diseases and for the study of gene function in vivo. We report the establishment of new lines of murine embryonic stem cells from preimplantation stage embryos of 129/Sv mice. Most of these cells had a normal karyotype and an XY sex chromosome composition. The pluripotent properties of the cell lines obtained were analyzed on the basis of their alkaline phosphatase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human genetic diseases; Murine embryonic stem cells; Mouse model; Transgenic mouse.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500004

Treatment with 1,25(OH)2D3 induced HDAC2 expression and reduced NF-κB p65 expression in a rat model of OVA-induced asthma
Provedor de dados: 56
Autores: Zhou,Y.; Wang,G.F.; Yang,L.; Liu,F.; Kang,J.Q.; Wang,R.L.; Gu,W.; Wang,C.Y.
Recent evidence indicates that a deficiency of 1,25-dihydroxyvitamin D3 (1,25[OH]2D3) may influence asthma pathogenesis; however, its roles in regulating specific molecular transcription mechanisms remain unclear. We aimed to investigate the effect of 1,25(OH)2D3 on the expression and enzyme activity of histone deacetylase 2 (HDAC2) and its synergistic effects with dexamethasone (Dx) in the inhibition of inflammatory cytokine secretion in a rat asthma model. Healthy Wistar rats were randomly divided into 6 groups: control, asthma, 1,25(OH)2D3 pretreatment, 1,25(OH)2D3 treatment, Dx treatment, and Dx and 1,25(OH)2D3 treatment. Pulmonary inflammation was induced by ovalbumin (OVA) sensitization and challenge (OVA/OVA). Inflammatory cells and cytokines in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 1; 25-Dihydroxyvitamin D3 Asthma Acetylation Deacetylation Histone deacetylase 2 NF-κB.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700654

microRNA-142-3p inhibits apoptosis and inflammation induced by bleomycin through down-regulation of Cox-2 in MLE-12 cells
Provedor de dados: 56
Autores: Guo,F.; Lin,S.C.; Zhao,M.S.; Yu,B.; Li,X.Y.; Gao,Q.; Lin,D.J.
microRNA (miR)-142-3p is implicated in malignancy and has been identified as a biomarker for aggressive and recurrent lung adenocarcinomas. This study aimed to evaluate the inhibitory effect of miR-142-3p on apoptosis and inflammation induced by bleomycin in MLE-12 cells. MLE-12 cells were first transfected either with miR-142-3p mimic or miR-142-3p inhibitor and then the cells were exposed to 50 μg/mL of bleomycin. Thereafter, cell viability, apoptosis and the expression of pro-inflammatory cytokines were assessed using CCK-8, flow cytometry, RT-PCR and western blot analyses. Cox-2, PI3K, AKT and mTOR expressions were detected by western blotting after bleomycin was administered together with NS-398 (an inhibitor of Cox-2). As a result, cell viability was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-142-3p; MLE-12 cells; Bleomycin; Apoptosis; Pro-inflammatory cytokines; Idiopathic pulmonary fibrosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700606

Anti-cyclic citrullinated peptide antibodies and rheumatoid factor in leprosy patients with articular involvement
Provedor de dados: 56
Autores: Ribeiro,S.L.E.; Pereira,H.L.A.; Silva,N.P.; Neves,R.M.S.; Sato,E.I.
The objective of the present research was to evaluate the usefulness of anti-cyclic citrullinated peptide (anti-CCP) antibodies and the IgM rheumatoid factor (IgM RF) test for the differential diagnosis of leprosy with articular involvement and rheumatoid arthritis (RA). Anti-CCP antibodies and IgM RF were measured in the sera of 158 leprosy patients (76 with and 82 without articular involvement), 69 RA patients and 89 healthy controls. Leprosy diagnosis was performed according to Ridley and Jopling classification criteria and clinical and demographic characteristics of leprosy patients were collected by a standard questionnaire. Leprosy patients with any concomitant rheumatic disease were excluded. Serum samples were obtained from all participants and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leprosy; Rheumatoid arthritis; Anti-cyclic citrullinated peptide antibodies; Rheumatoid factor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100010

Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
Provedor de dados: 56
Autores: Puscas,I.; Coltau,M.; Gilau,L.; Pasca,R.; Domuta,G.; Baican,M.; Hecht,A.
We studied the relationship between alpha- and beta-adrenergic agonists and the activity of carbonic anhydrase I and II in erythrocyte, clinical and vessel studies. Kinetic studies were performed. Adrenergic agonists increased erythrocyte carbonic anhydrase as follows: adrenaline by 75%, noradrenaline by 68%, isoprenaline by 55%, and orciprenaline by 62%. The kinetic data indicated a non-competitive mechanism of action. In clinical studies carbonic anhydrase I from erythrocytes increased by 87% after noradrenaline administration, by 71% after orciprenaline and by 82% after isoprenaline. The increase in carbonic anhydrase I paralleled the increase in blood pressure. Similar results were obtained in vessel studies on piglet vascular smooth muscle. We believe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenergic agonists; Carbonic anhydrase; Arterial blood pressure.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300007

Low expression of antigen-presenting and costimulatory molecules by lung cells from tuberculosis patients
Provedor de dados: 56
Autores: Flores-Batista,V.C.S.; Boechat,N.; Lago,P.M.; Lazzarini,L.C.; Pessanha,L.R.; Almeida,A.S.; Mafort,T.T.; Kritski,A.L.; Ho,J.L.; Lapa-e-Silva,J.R.
Costimulatory and antigen-presenting molecules are essential to the initiation of T cell immunity to mycobacteria. The present study analyzed by immunocytochemistry, using monoclonal antibodies and alkaline phosphatase-anti-alkaline phosphatase method, the frequency of costimulatory (CD86, CD40, CD40L, CD28, and CD152) and antigen-presenting (MHC class II and CD1) molecules expression on human lung cells recovered by sputum induction from tuberculosis (TB) patients (N = 22) and non-TB controls (N = 17). TB cases showed a statistically significant lower percentage of HLA-DR+ cells than control subjects (21.9 ± 4.2 vs 50.0 ± 7.2%, P &lt; 0.001), even though similar proportions of TB cases (18/22) and control subjects (16/17, P = 0.36) had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Costimulatory molecules; CD86; MHC class II; HLA-DR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200011

Acute effects of resistance exercise and intermittent intense aerobic exercise on blood cell count and oxidative stress in trained middle-aged women
Provedor de dados: 56
Autores: Cardoso,A.M.; Bagatini,M.D.; Roth,M.A.; Martins,C.C.; Rezer,J.F.P.; Mello,F.F.; Lopes,L.F.D.; Morsch,V.M.; Schetinger,M.R.C.
The aim of this study was to compare the effect of an intermittent intense aerobic exercise session and a resistance exercise session on blood cell counts and oxidative stress parameters in middle-aged women. Thirty-four women were selected and divided into three groups: RE group (performing 60 min of resistance exercises, N = 12), spinning group (performing 60 min of spinning, N = 12), and control group (not exercising regularly, N = 10). In both exercise groups, lymphocytes and monocytes decreased after 1-h recuperation (post-exercise) compared to immediately after exercise (P &lt; 0.05). Immediately after exercise, in both exercised groups, a significant increase in TBARS (from 16.5 ± 2 to 25 ± 2 for the spinning group and from 18.6 ± 1 to 28.2 ± 3 nmol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intermittent exercise; Anaerobic exercise; Oxidative stress; Immune suppression; Middle-aged women.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200010

Characterization of bovine respiratory syncytial virus isolated in Brazil
Provedor de dados: 56
Autores: Arns,C.W.; Campalans,J.; Costa,S.C.B.; Domingues,H.G.; D'Arce,R.C.F.; Almeida,R.S.; Coswig,L.T.
This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and non-hemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine respiratory syncytial virus; BRSV; Virus isolation; Characterization; Nested RT-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200008

Serum cytokine levels in autoimmune and non-autoimmune hyperthyroid states
Provedor de dados: 56
Autores: Ward,L.S.; Fernandes,G.A.
Although the role of interleukin-2 (IL-2) and interferon gamma (&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;IFN) is still poorly understood in hyperthyroid diseases, it is reasonable to assume that these cytokines may be present at higher levels in Graves' disease (GD) than in other primarily non-autoimmune thyroid diseases. In order to look for an easy method to distinguish GD from primarily non-autoimmune causes of hyperthyroidism, we compared 13 healthy individuals with 21 treated and untreated hyperthyroid GD patients and with 19 patients with hyperthyroidism due to other etiologies: 7 cases of multinodular goiter, 5 cases of excessive hormone replacement and 7 cases of amiodarone-associated hyperthyroidism. All patients presented low TSH levels and a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokines; IL-2; Hyperthyroidism; Graves' disease; Diagnosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100009

The role of stress-activated protein kinase signaling in renal pathophysiology
Provedor de dados: 56
Autores: Ma,F.Y.; Liu,J.; Nikolic-Paterson,D.J.
Two major stress-activated protein kinases are the p38 mitogen-activated protein kinase (MAPK) and the c-Jun amino terminal kinase (JNK). p38 and JNK are widely expressed in different cell types in various tissues and can be activated by a diverse range of stimuli. Signaling through p38 and JNK is critical for embryonic development. In adult kidney, p38 and JNK signaling is evident in a restricted pattern suggesting a normal physiological role. Marked activation of both p38 and JNK pathways occurs in human renal disease, including glomerulonephritis, diabetic nephropathy and acute renal failure. Administration of small molecule inhibitors of p38 and JNK has been shown to provide protection from renal injury in different types of experimental kidney disease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P38; C-Jun amino terminal kinase; Inflammation; Fibrosis; Apoptosis; Renal pathophysiology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100006

Narrow-band 1, 2, 3, 4, 8, 16 and 24 cycles/360º angular frequency filters
Provedor de dados: 56
Autores: Simas,M.L.B.; Santos,N.A.
We measured human frequency response functions for seven angular frequency filters whose test frequencies were centered at 1, 2, 3, 4, 8, 16 or 24 cycles/360º using a supra-threshold summation method. The seven functions of 17 experimental conditions each were measured nine times for five observers. For the arbitrarily selected filter phases, the maximum summation effect occurred at test frequency for filters at 1, 2, 3, 4 and 8 cycles/360º. For both 16 and 24 cycles/360º test frequencies, maximum summation occurred at the lower harmonics. These results allow us to conclude that there are narrow-band angular frequency filters operating somehow in the human visual system either through summation or inhibition of specific frequency ranges. Furthermore, as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spatial frequency; Angular frequency; Narrow-band frequency filters; Polar gratings; Radial frequency.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200014

Atrial natriuretic peptide and feeding activity patterns in rats
Provedor de dados: 56
This review presents historical data about atrial natriuretic peptide (ANP) from its discovery as an atrial natriuretic factor (ANF) to its role as an atrial natriuretic hormone (ANH). As a hormone, ANP can interact with the hypothalamic-pituitary-adrenal axis (HPA-A) and is related to feeding activity patterns in the rat. Food restriction proved to be an interesting model to investigate this relationship. The role of ANP must be understood within a context of peripheral and central interactions involving different peptides and pathways
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Atrial natriuretic peptide; Corticosterone; ACTH; Food restriction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400006

Ca2+ signaling in pancreatic acinar cells: physiology and pathophysiology
Provedor de dados: 56
Autores: Petersen,O.H.
The pancreatic acinar cell is a classical model for studies of secretion and signal transduction mechanisms. Because of the extensive endoplasmic reticulum and the large granular compartment, it has been possible - by direct measurements - to obtain considerable insights into intracellular Ca2+ handling under both normal and pathological conditions. Recent studies have also revealed important characteristics of stimulus-secretion coupling mechanisms in isolated human pancreatic acinar cells. The acinar cells are potentially dangerous because of the high intra-granular concentration of proteases, which become inappropriately activated in the human disease acute pancreatitis. This disease is due to toxic Ca2+ signals generated by excessive liberation of Ca2+...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+ signaling; Pancreatic acinar cells; Stimulus-secretion coupling; Human acinar cells; Ca2+ entry; Ca2+ extrusion.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100003

Gender-related differences in the effects of nitric oxide donors on neuroleptic-induced catalepsy in mice
Provedor de dados: 56
Autores: Pires,J.G.P.; Costa,P.G.; Saraiva,F.P.; Bonikovski,V.; Futuro Neto,H.A.
It has been suggested that nigrostriatal dopaminergic transmission is modulated by nitric oxide (NO). Since there is evidence that gonadal hormones can affect extrapyramidal motor behavior in mammals, we investigated the effects of isosorbide dinitrate (ISD), linsidomine (SIN-1) and S-nitroso-N-acetylpenicillamine (SNAP), three pharmacologically different NO donors, on neuroleptic-induced catalepsy in 60- to 80-day-old male and female albino mice. Catalepsy was induced with haloperidol (1 mg/kg, ip) and measured at 30-min intervals by means of a bar test. Drugs (or appropriate vehicle) were injected ip 30 min before haloperidol, with each animal being used only once. ISD (5, 20 and 50 mg/kg) caused a dose-dependent inhibition of catalepsy in male mice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroleptic-induced catalepsy; Nitric oxide donors; Gender differences; Linsidomine; SNAP; Isosorbide dinitrate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200012

Factors associated with Clostridium difficile diarrhea in a hospital in Beijing, China
Provedor de dados: 56
Autores: Lv,Z.; Peng,G.L.; Su,J.R.
Clostridium difficile is the most common cause of hospital-acquired diarrhea in patients treated with antibiotics, chemotherapeutic agents, and other drugs that alter the normal equilibrium of the intestinal flora. A better understanding of the risk factors for C. difficile-associated disease (CDAD) could be used to reduce the incidence of CDAD and the costs associated with its treatment. The aim of this study was to identify the risk factors for CDAD in a cohort of Chinese patients in a Beijing hospital. Medical charts of a total of 130 inpatients (62 males and 68 females) with hospital-acquired diarrhea (45 with CDAD; 85 without CDAD) were retrospectively reviewed. C. difficile toxins A and B were detected in fecal samples using enzyme-linked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics; Clostridium difficile; Glycopeptides.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201085

Vascular complications of black patients with type 2 diabetes mellitus in Southern Brazil
Provedor de dados: 56
Autores: Gerchman,F.; Zanatta,C.M.; Burttet,L.M.; Picon,P.X.; Lisboa,H.R.K.; Silveiro,S.P.; Gross,J.L.; Canani,L.H.
Ethnicity has been shown to be associated with micro- and macrovascular complications of diabetes in European and North American populations. We analyzed the contribution of ethnicity to the prevalence of micro- and macrovascular complications in Brazilian subjects with type 2 diabetes attending the national public health system. Data from 1810 subjects with type 2 diabetes (1512 whites and 298 blacks) were analyzed cross-sectionally. The rates of ischemic heart disease, peripheral vascular disease, stroke, distal sensory neuropathy, and diabetic retinopathy were assessed according to self-reported ethnicity using multiple logistic regression models. Compared to whites, black subjects [odds ratio = 1.72 (95%CI = 1.14-2.6)] were more likely to have ischemic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethnicity; Diabetes mellitus; Chronic complications.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800005

Radial frequency stimuli and sine-wave gratings seem to be processed by distinct contrast brain mechanisms
Provedor de dados: 56
Autores: Simas,M.L.B.; Nogueira,R.M.T.B.L.; Santos,N.A.
An assumption commonly made in the study of visual perception is that the lower the contrast threshold for a given stimulus, the more sensitive and selective will be the mechanism that processes it. On the basis of this consideration, we investigated contrast thresholds for two classes of stimuli: sine-wave gratings and radial frequency stimuli (i.e., j0 targets or stimuli modulated by spherical Bessel functions). Employing a suprathreshold summation method, we measured the selectivity of spatial and radial frequency filters using either sine-wave gratings or j0 target contrast profiles at either 1 or 4 cycles per degree of visual angle (cpd), as the test frequencies. Thus, in a forced-choice trial, observers chose between a background spatial (or radial)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sine-wave gratings; Spatial frequency; Radial frequency; Bessel functions; Multiple channels; Contrast sensitivity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300013

Effect of suramin on myotoxicity of some crotalid snake venoms
Provedor de dados: 56
Autores: Arruda,E.Z.; Silva,N.M.V.; Moraes,R.A.M.; Melo,P.A.
We investigated the protective effect of suramin, an enzyme inhibitor and an uncoupler of G protein from receptors, on the myotoxic activity in mice of different crotalid snake venoms (A.c. laticinctus, C.v. viridis, C.d. terrificus, B. jararacussu, B. moojeni, B. alternatus, B. jararaca, L. muta). Myotoxicity was evaluated in vivo by injecting im the venoms (0.5 or 1.0 mg/kg) dissolved in physiological saline solution (0.1 ml) and measuring plasma creatine kinase (CK) activity. Two experimental approaches were used in mice (N = 5 for each group). In protocol A, 1 mg of each venom was incubated with 1.0 mg suramin (15 min, 37ºC, in vitro), and then injected im into the mice at a dose of 1.0 mg/kg (in vivo). In protocol B, venoms, 1.0 mg/kg, were injected...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snake venom; Myotoxicity; Creatine kinase; Suramin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600013

Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
Provedor de dados: 56
Autores: Andrade,A.Q.; Casarini,D.E.; Schor,N.; Boim,M.A.
Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin; Prorenin; Mesangial cell; Kidney.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100003

Potential benefit of (-)-epigallocatechin-3-gallate for macrovascular complications in diabetes
Provedor de dados: 56
Autores: Tang,L.; Li,L.; Yang,J.; Zeng,C.
Vascular problems are the most common complications in diabetes. Substantial evidence from epidemiological and pathophysiological studies show that hyperglycemia is a major risk factor for macrovascular complications in patients with diabetes. (-)-Epigallocatechin-3-gallate (EGCG), the major catechin derived from green tea, is known to exert a variety of cardiovascular beneficial effects. The protective effects of EGCG in diabetes are also evident. However, whether EGCG is beneficial against macrovascular complications that occur in diabetes remains unknown. Our previous studies demonstrated that treatment of EGCG inhibits high glucose-induced vascular smooth muscle cell proliferation and suppresses high glucose-mediated vascular inflammation in human...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: (-)-Epigallocatechin-3-gallate; Diabetes; Macrovascular complications; Diabetes complications; Hypothesis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000401

The corporate bias and the molding of prescription practices: the case of hypertension
Provedor de dados: 56
Autores: Fuchs,F.D.
Drug management of hypertension has been a noticeable example of the influence of the pharmaceutical industry on prescription practices. The worldwide leading brands of blood pressure-lowering agents are angiotensin receptor-blocking agents, although they are considered to be simply substitutes of angiotensin-converting enzyme (ACE) inhibitors. Commercial strategies have been based on the results of clinical trials sponsored by drug companies. Most of them presented distortions in their planning, presentation or interpretation that favored the drugs from the sponsor, i.e., corporate bias. Atenolol, an ineffective blood pressure agent in elderly individuals, was the comparator drug in several trials. In a re-analysis of the INSIGHT trial, deaths appeared to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hypertension; Blood pressure agents; Clinical trials; Ethics; Corporate bias.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300002

High dietary calcium decreases blood pressure in normotensive rats
Provedor de dados: 56
Autores: Buassi,N.
This study evaluates the influence of different concentrations of calcium on blood pressure of normotensive rats. Four groups of Wistar rats (A, B, C and D) had free access to modified isocaloric and isoproteic diets containing 0.2, 0.5, 2 and 4 g% calcium as calcium carbonate for a period of 30 days. Systolic and diastolic arterial blood pressures were monitored in awake rats by the indirect tail cuff method using a Physiograph equipped with transducers and preamplifiers. Body weight and length and food intake were monitored. Under the conditions of the present experiment, the systolic and diastolic arterial blood pressures of group D rats fed a diet containing 4 g% calcium were significantly (P&amp;lt;0.05) lower compared to rats of the other groups.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Calcium carbonate; Arterial blood pressure; Dietary calcium.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800011

Biological evaluation of recombinant human erythropoietin in pharmaceutical products
Provedor de dados: 56
Autores: Ramos,A.S.; Schmidt,C.A.; Andrade,S.S.; Fronza,M.; Rafferty,B.; Dalmora,S.L.
The potencies of mammalian cell-derived recombinant human erythropoietin pharmaceutical preparations, from a total of five manufacturers, were assessed by in vivo bioassay using standardized protocols. Eight-week-old normocythemic mice received a single subcutaneous injection followed by blood sampling 96 h later or multiple daily injections with blood sampling 24 h after the last injection. Reticulocyte counting by microscopic examination was employed as the end-point using the brilliant cresyl blue or selective hemolysis methods, together with automated flow cytometry. Different injection schedules were investigated and dose-response curves for the European Pharmacopoeia Biological Reference Preparation of erythropoietin were compared. Manual and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant human erythropoietin; Bioassay; Polycythemia; Reticulocyte counting; Normocythemic mice.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100014

Fish oil ingestion reduces the number of aberrant crypt foci and adenoma in 1,2-dimethylhydrazine-induced colon cancer in rats
Provedor de dados: 56
Autores: Moreira,A.P.B.; Sabarense,C.M.; Dias,C.M.G.C.; Lunz,W.; Natali,A.J.; Glória,M.B.A.; Peluzio,M.C.G.
We determined the effect of fish oil (FO) ingestion on colonic carcinogenesis in rats. Male Wistar rats received 4 subcutaneous injections (40 mg/kg body weight each) of 1,2-dimethylhydrazine (DMH) at 3-day intervals and were fed a diet containing 18% by weight FO (N = 10) or soybean oil (SO, N = 10) for 36 weeks. At sacrifice, the colon was removed, aberrant crypt foci were counted and the fatty acid profile was determined. Intestinal tumors were removed and classified as adenoma or carcinoma. Liver and feces were collected and analyzed for fatty acid profile. FO reduced the mean (± SEM) number of aberrant crypt foci compared to SO (113.55 ± 6.97 vs 214.60 ± 18.61; P &lt; 0.05) and the incidence of adenoma (FO: 20% vs SO: 100%), but carcinoma occurred...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colonic aberrant crypt foci; Colorectal cancer prevention; Colorectal tumor; Omega-3 fatty acids; Polyamines.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200008

IS1245 restriction fragment length polymorphism typing of Mycobacterium avium from patients admitted to a reference hospital in Campinas, Brazil
Provedor de dados: 56
Autores: Panunto,A.C.; Villares,M.C.B.; Ramos,M.C.
Mycobacterium avium is an important pathogen among immunodeficient patients, especially patients with AIDS. The natural history of this disease is unclear. Several environmental sources have been implicated as the origin of this infection. Polyclonal infection with this species is observed, challenging the understanding of its pathogenesis and treatment. In the present study 45 M. avium strains were recovered from 39 patients admitted to a reference hospital between 1996 and 1998. Species identification was performed using a species-specific nucleic acid hybridization test (AccuProbe®) from Gen-Probe®. Strains were genotyped using IS1245 restriction fragment length polymorphism typing. Blood was the main source of the organism. In one patient with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium avium; IS1245 RFLP; Mycobacteria; AIDS; Genotyping.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000017

Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the mycobacteria growth indicator tube (MGIT) system
Provedor de dados: 56
Autores: Telles,M.A.S.; Bori,A.; Amorim,A.B.R.; Cruz,A.F.; Pini,M.I.T.; Sato,D.N.
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis has increased the need for rapid drug susceptibility tests, which are needed for adequate patient treatment. The objective of the present study was to evaluate the mycobacteria growth indicator tube (MGIT) system to detect multidrug-resistant M. tuberculosis strains. The MGIT system was compared with two standard methods (proportion and resistance ratio methods). One hundred clinical M. tuberculosis isolates [25 susceptible to isoniazid (INH) and rifampicin (RIF), 20 resistant to INH, 30 resistant to INH-RIF, and 25 resistant to INH-RIF and other drugs] obtained in the State of São Paulo were tested for INH and RIF susceptibility. Full agreement among the tests was found for all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multidrug-resistant Mycobacterium tuberculosis; Susceptibility tests; Mycobacteria growth indicator tube; Proportion method; Resistance ratio method; Multidrug resistance.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000003

Semi-automatic measurement of visual verticality perception in humans reveals a new category of visual field dependency
Provedor de dados: 56
Autores: Kaleff,C.R.; Aschidamini,C.; Baron,J.; Leone,C.N. de; Canavarro,S.; Vargas,C.D.
Previous assessment of verticality by means of rod and rod and frame tests indicated that human subjects can be more (field dependent) or less (field independent) influenced by a frame placed around a tilted rod. In the present study we propose a new approach to these tests. The judgment of visual verticality (rod test) was evaluated in 50 young subjects (28 males, ranging in age from 20 to 27 years) by randomly projecting a luminous rod tilted between -18 and +18° (negative values indicating left tilts) onto a tangent screen. In the rod and frame test the rod was displayed within a luminous fixed frame tilted at +18 or -18°. Subjects were instructed to verbally indicate the rod’s inclination direction (forced choice). Visual dependency was estimated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rod test; Rod and frame test; Subjective visual vertical; Visual streams; Two-alternative forced-choice task.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800004

Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage
Provedor de dados: 56
Autores: Ni,X.J.; Xu,Z.Q.; Jin,H.; Zheng,S.L.; Cai,Y.; Wang,J.J.
Ginsenoside Rg1, one of the most notable active components of Panax ginseng, has been widely reported to exert anti-inflammatory actions. This study aimed to reveal whether ginsenoside Rg1 also exhibits beneficial roles against lipopolysaccharide (LPS)-induced apoptosis and inflammation in human renal tubular epithelial cells, and to evaluate the potential role of the component on tubulointerstitial nephritis treatment. HK-2 cells were treated with various doses of ginsenoside Rg1 (0, 50, 100, 150, and 200 μM) in the absence or presence of 5 μg/mL LPS. Thereafter, CCK-8 assay, flow cytometry, western blot, migration assay, reactive oxygen species (ROS) assay, and ELISA were carried out to respectively assess cell viability, apoptosis, migration, ROS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginsenoside Rg1; LPS; Apoptosis; Inflammation; PI3K/AKT pathway; NF-κB pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200608

Effect of Lactobacillus delbrueckii on cholesterol metabolism in germ-free mice and on atherogenesis in apolipoprotein E knock-out mice
Provedor de dados: 56
Autores: Portugal,L.R.; Gonçalves,J.L.; Fernandes,L.R.; Silva,H.P.S.; Arantes,R.M.E.; Nicoli,J.R.; Vieira,L.Q.; Alvarez-Leite,J.I.
Elevated blood cholesterol is an important risk factor associated with atherosclerosis and coronary heart disease. Several studies have reported a decrease in serum cholesterol during the consumption of large doses of fermented dairy products or lactobacillus strains. The proposed mechanism for this effect is the removal or assimilation of intestinal cholesterol by the bacteria, reducing cholesterol absorption. Although this effect was demonstrated in vitro, its relevance in vivo is still controversial. Furthermore, few studies have investigated the role of lactobacilli in atherogenesis. The aim of the present study was to determine the effect of Lactobacillus delbrueckii on cholesterol metabolism in germ-free mice and the possible hypocholesterolemic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Atherosclerosis; Apolipoprotein E; Cholesterol; Germ-free mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500010

Karyotype of cryopreserved bone marrow cells
Provedor de dados: 56
Autores: Chauffaille,M.L.L.F.; Pinheiro,R.F.; Stefano,J.T.; Kerbauy,J.
The analysis of chromosomal abnormalities is important for the study of hematological neoplastic disorders since it facilitates classification of the disease. The ability to perform chromosome analysis of cryopreserved malignant marrow or peripheral blast cells is important for retrospective studies. In the present study, we compared the karyotype of fresh bone marrow cells (20 metaphases) to that of cells stored with a simplified cryopreservation method, evaluated the effect of the use of granulocyte-macrophage colony-stimulating factor (GM-CSF) as an in vitro mitotic index stimulator, and compared the cell viability and chromosome morphology of fresh and cryopreserved cells whenever possible (sufficient metaphases for analysis). Twenty-five bone marrow...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cryopreservation; Karyotype; Bone marrow cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700004

Association between varicose veins anatomical pattern and procedural complications following endovascular laser photothermolysis for chronic venous insufficiency
Provedor de dados: 56
Autores: Molnar,C.; Opincariu,D.; Benedek,T.; Toma,M.; Nicolescu,C.
We sought to assess clinical characteristics and pattern of collateral network involvement associated with development of truncal (systematized) versus diffuse/non-truncal (non-systematized) varicose veins (VVs) in patients undergoing endovascular laser photothermolysis for chronic venous insufficiency (CVI). Secondly, we aimed to assess whether the type of VVs influenced the procedural complications of endovascular laser therapy. A total of 508 patients with hydrostatic VVs of the lower limbs who underwent endovenous laser treatment were included, out of which 84.1% (n=427) had truncal VVs (group 1) and 15.9% (n=81) had diffuse (non-systematized) VVs (group 2). Patients with truncal varices were significantly older (47.50±12.80 vs 43.15±11.75 years,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Varicose veins; Venous insufficiency; Laser phlebectomy; Truncal veins.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400606

Distribution of the a2, a3, and a5 nicotinic acetylcholine receptor subunits in the chick brain
Provedor de dados: 56
Autores: Torrão,A.S.; Lindstrom,J.M.; Britto,L.R.G.
Nicotinic acetylcholine receptors (nAChRs) are ionotropic receptors comprised of &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; and ß subunits. These receptors are widely distributed in the central nervous system, and previous studies have revealed specific patterns of localization for some nAChR subunits in the vertebrate brain. In the present study we used immunohistochemical methods and monoclonal antibodies to localize the &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2, &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;3, and &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;5 nAChR subunits in the chick mesencephalon and diencephalon. We observed a differential distribution of these three subunits in the chick brain, and showed that the somata and neuropil of many central...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholinergic system; Neurotransmitters; Receptors; Receptor subunits.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000011

Administration of interferon-g to pregnant rats reverses the depressed adjuvant-induced arthritis of their chronically Trypanosoma cruzi-infected offspring
Provedor de dados: 56
Autores: Didoli,G.; Revelli,S.; Davila,H.; Ferro,M.E.; Romero-Piffiguer,M.; Bottasso,O.
We demonstrated that administration of interferon gamma (IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;) to the inbred "l" strain of pregnant rats conferred partial resistance on their offspring to challenge with Trypanosoma cruzi. We now examine if this intervention also modifies the reportedly immunodepressed cellular responses which occur during chronic infection. Offspring were born to mothers undergoing one of the following procedures during gestation: subcutaneous injections of recombinant rat IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;, 50,000 IU/rat, five times/week for 3 weeks, which was started on the day of mating (IFN-Mo); infection with 106 trypomastigotes of T. cruzi at 7, 14, and 21 days after mating plus IFN-&lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon gamma; Adjuvant arthritis; T. cruzi; T cell subsets.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600011

Evaluation of the antischistosomal activity of sulfated α-D-glucan from the lichen Ramalina celastri free and encapsulated into liposomes
Provedor de dados: 56
Autores: Araújo,R.V.S.; Melo-Júnior,M.R.; Beltrão,E.I.C.; Mello,L.A.; Iacomini,M.; Carneiro-Leão,A.M.A.; Carvalho Jr,L.B.; Santos-Magalhães,N.S.
The antischistosomal activity of the sulfated polysaccharide α-D-glucan (Glu.SO4) extracted from Ramalina celastri was evaluated after encapsulation into liposomes (Glu.SO4-LIPO) in Schistosoma mansoni-infected mice. The effect of treatment with Glu.SO4 and Glu.SO4-LIPO (10 mg/kg) on egg elimination, worm burden and hepatic granuloma formation was assessed using female albino Swiss mice, 35-40 days of age, weighing 25 ± 2 g, infected with 150 cercariae/animal (Biomphalaria glabrata, BH strain). Four groups (N = 10) were studied, two controls (empty liposomes and NaCl) and two treated groups (Glu.SO4-LIPO and Glu.SO4) using a single dose. Parasitological analysis revealed that Glu.SO4-LIPO was as efficient as Glu.SO4 in reducing egg elimination and worm...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polysaccharide; Α-D-glucan; Ramalina celastri; Liposomes; Schistosoma mansoni; Lectin histochemistry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400007

Effects of naltrexone and cross-tolerance to morphine in a learned helplessness paradigm
Provedor de dados: 56
Opiates have been implicated in learned helplessness (LH), a phenomenon known to be related to opiate stress-induced analgesia (SIA). In the present study, we investigated the role of opiates in the induction of LH and SIA under different conditions. Adult female Wistar rats were trained either by receiving 60 inescapable 1-mA footshocks (IS group, N = 114) or by confinement in the shock box (control or NS group, N = 92). The pain threshold of some of the animals was immediately evaluated in a tail-flick test while the rest were used 24 h later in a shuttle box experiment to examine their escape performance. The opiate antagonist naltrexone (0 or 8 mg/kg, ip) and the previous induction of cross-tolerance to morphine by the chronic administration of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Opiates; Stress-induced analgesia; Learned helplessness; Depression.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600012

Early remodeling of rat cardiac muscle induced by swimming training
Provedor de dados: 56
Autores: Verzola,R.M.M.; Mesquita,R.A.; Peviani,S.; Ramos,O.H.P.; Moriscot,A.S.; Perez,S.E.A.; Selistre-de-Araújo,H.S.
The aim of the present investigation was to study the effect of acute swimming training with an anaerobic component on matrix metallopeptidase (MMP) activity and myosin heavy chain gene expression in the rat myocardium. Animals (male Wistar rats, weighing approximately 180 g) were trained for 6 h/day in 3 sessions of 2 h each for 1 to 5 consecutive days (N = 5 rats per group). Rats swam in basins 47 cm in diameter and 60 cm deep filled with water at 33 to 35ºC. After the training period a significant increase (P &lt; 0.05) was observed in the heart weight normalized to body weight by about 22 and 35% in the groups that trained for 96 and 120 h, respectively. Blood lactate levels were significantly increased (P &lt; 0.05) in all groups after all training...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myosin heavy chain expression; Training; Real time PCR; Rat myocardium; Muscle hypertrophy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500009

Control of respiration in fish, amphibians and reptiles
Provedor de dados: 56
Autores: Taylor,E.W.; Leite,C.A.C.; McKenzie,D.J.; Wang,T.
Fish and amphibians utilise a suction/force pump to ventilate gills or lungs, with the respiratory muscles innervated by cranial nerves, while reptiles have a thoracic, aspiratory pump innervated by spinal nerves. However, fish can recruit a hypobranchial pump for active jaw occlusion during hypoxia, using feeding muscles innervated by anterior spinal nerves. This same pump is used to ventilate the air-breathing organ in air-breathing fishes. Some reptiles retain a buccal force pump for use during hypoxia or exercise. All vertebrates have respiratory rhythm generators (RRG) located in the brainstem. In cyclostomes and possibly jawed fishes, this may comprise elements of the trigeminal nucleus, though in the latter group RRG neurons have been located in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vertebrates; Control of respiration; Respiratory rhythm generation; Water and air breathing; Chemoreceptors; Mechanoreceptors.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500001

Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model
Provedor de dados: 56
Autores: Xu,Y.; Song,Y.F.; Lin,Z.X.
We investigated whether fibrin glue (FG) could promote urethral sphincter restoration in muscle-derived stem cell (MDSC)-based injection therapies in a pudendal nerve-transected (PNT) rat, which was used as a stress urinary incontinence (SUI) model. MDSCs were purified from the gastrocnemius muscles of 4-week-old inbred female SPF Wistar rats and labeled with green fluorescent protein. Animals were divided into five groups (N = 15): sham (S), PNT (D), PNT+FG injection (F), PNT+MDSC injection (M), and PNT+MDSC+FG injection (FM). Each group was subdivided into 1- and 4-week groups. One and 4 weeks after injection into the proximal urethra, leak point pressure (LPP) was measured to assess urethral resistance function. Histology and immunohistochemistry were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle-derived stem cells; Fibrin glue; Urinary incontinence; Cellular therapy; Pudendal nerve transection.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100009

The N-terminal 33 amino acid domain of Siva-1 is sufficient for nuclear localization
Provedor de dados: 56
Autores: Chen,J.Y.; Yang,L.X.; Huang,Z.F.
Siva-1 induces apoptosis in multiple pathological processes and plays an important role in the suppression of tumor metastasis, protein degradation, and other functions. Although many studies have demonstrated that Siva-1 functions in the cytoplasm, a few have found that Siva-1 can relocate to the nucleus. In this study, we found that the first 33 amino acid residues of Siva-1 are required for its nuclear localization. Further study demonstrated that the green fluorescent protein can be imported into the nucleus after fusion with these 33 amino acid residues. Other Siva-1 regions and domains showed less effect on Siva-1 nuclear localization. By site-mutagenesis of all of these 33 amino acid residues, we found that mutants of the first 1-18 amino acids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Siva-1; Nuclear localization signal (NLS); Subcellular compartmentalization; Nuclear localization.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201021

Mesopic radial frequency contrast sensitivity function for young and older adults
Provedor de dados: 56
Autores: Santos,N.A.; Oliveira,A.B.; Nogueira,R.M.T.B.L.; Simas,M.L.B.
The objective of the present study was to determine contrast sensitivity curves of concentric circular patterns with radial frequencies of 0.25, 0.5, 1.0, 2.0, and 4.0 cycles per degree in young and older adult volunteers. These parameters were also compared with sensitivity contrasts for sine-wave gratings. All participants had normal acuity vision and were free of identifiable ocular illness. Contrast sensitivity was measured in 6 young adults aged 19 to 23 years and 6 older adults aged 60 to 69 years using the psychophysical forced-choice method. In this paradigm the volunteers had to decide which of two stimuli contained the above radial frequencies at low contrast levels. The other neutral stimulus was gray with homogeneous luminance. We detected a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Visual form processing; Radial frequency; Contrast sensitivity; Forced-choice method.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600012

Preoperative cobalt60 irradiation delays the healing of rectal anastomoses in rats
Provedor de dados: 56
Autores: Fang,C.B.; Klug,W.A.; Capelhuchnik,P.
The healing of colorectal anastomoses after irradiation therapy continues to be a major concern. The authors evaluated the healing of rectal anastomoses in a rat model after a preoperative 500-cGy dose of cobalt60 irradiation. Thirty-six male Wistar rats were divided into two equal groups: control (group A), and irradiation group (group B). Group B received a single 500-cGy dose of irradiation, and a rectal resection and end-to-end anastomosis was performed in both groups on the 7th day after irradiation. Parameters of the healing process included bursting pressure and collagen content on the 5th, 7th, and 14th days after surgery. In the irradiation group, the mean bursting pressure on the 5th, 7th, and 14th days was 116, 218, and 273 mmHg, respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irradiation; Healing; Rectum.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600011

Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis
Provedor de dados: 56
The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac+; another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants...
Palavras-chave: Tetracycline; Mutation; LacZ-fusion; Proteus mirabilis; Bacteriophage Mu.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300009

Prolonged acceptance of skin grafts induced by B cells places regulatory T cells on the histopathology scene
Provedor de dados: 56
Autores: Langier,S.; Galvani,R.G.; Alves,A.P.G.; Fidelis,R.; Nunes,P.H.C.; Silva,M.H.; Castilho,L.R.; Monteiro,J.P.; Bonomo,A.
The participation of regulatory T (Treg) cells in B cell-induced T cell tolerance has been claimed in different models. In skin grafts, naive B cells were shown to induce graft tolerance. However, neither the contribution of Treg cells to B cell-induced skin tolerance nor their contribution to the histopathological diagnosis of graft acceptance has been addressed. Here, using male C57BL/6 naive B cells to tolerize female animals, we show that skin graft tolerance is dependent on CD25+ Treg cell activity and independent of B cell-derived IL-10. In fact, B cells from IL-10-deficient mice were able to induce skin graft tolerance while Treg depletion of the host inhibited 100% graft survival. We questioned how Treg cell-mediated tolerance would impact on...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skin graft; Tolerance; B cells; Treg; Histopathology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000009

Angiotensin-(1–7) inhibits inflammation and oxidative stress to relieve lung injury induced by chronic intermittent hypoxia in rats
Provedor de dados: 56
Autores: Lu,W.; Kang,J.; Hu,K.; Tang,S.; Zhou,X.; Yu,S.; Li,Y.; Xu,L.
Obstructive sleep apnea is associated with inflammation and oxidative stress in lung tissues and can lead to metabolic abnormalities. We investigated the effects of angiotensin1–7 [Ang-(1–7)] on lung injury in rats induced by chronic intermittent hypoxia (CIH). We randomly assigned 32 male Sprague-Dawley rats (180–200 g) to normoxia control (NC), CIH-untreated (uCIH), Ang-(1–7)-treated normoxia control (N-A), and Ang-(1–7)-treated CIH (CIH-A) groups. Oxidative stress biomarkers were measured in lung tissues, and expression of NADPH oxidase 4 (Nox4) and Nox subunits (p22phox, and p47phox) was determined by Western blot and reverse transcription-polymerase chain reaction. Pulmonary pathological changes were more evident in the uCIH group than in the other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ang-(1–7); Lung injury; Obstructive sleep apnea; Inflammation; Oxidative stress; Chronic intermittent hypoxia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000603

The inhibitory effect of photodynamic therapy and of an anti-VCAM-1 monoclonal antibody on the in vivo growth of C6 glioma xenografts
Provedor de dados: 56
Autores: Zhan,Qi; Yue,Wu; Shaoshan,Hu.
We investigated the effect of photodynamic therapy (PDT) and of an anti-vascular cell adhesion molecule-1 (VCAM-1) monoclonal antibody on the in vivo growth of C6 glioma. Seven days after inoculation with C6 cells, adult male Wistar rats weighing 280-300 g with MRI-confirmed glioma were randomly assigned to 4 groups (N = 15 per group): PDT + VCAM-1 antibody group; PDT group; VCAM-1 antibody group; control group. Eight days after inoculation, hematoporphyrin monomethyl ether (HMME) was administered as a photosensitizer and PDT was performed at 630 nm (illumination intensity: 360 J/cm²) for 10 min. VCAM-1 antibody (50 µg/mL) was then administered (0.5 mL) through the tail vein every other day from day 8 to day 16. At day 21, 5 rats in each group were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C6 glioma cells; Photodynamic therapy; Hematoporphyrin monomethyl ether; Anti-VCAM-1 monoclonal antibody; Apoptosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500016

Effects of microcystin-LR in isolated perfused rat kidney
Provedor de dados: 56
Autores: Nobre,A.C.L.; Jorge,M.C.M.; Menezes,D.B.; Fonteles,M.C.; Monteiro,H.S.A.
Microcystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR (1 µg/ml) was perfused over a period of 120 min, during which samples of urine and perfusate were collected at 10-min intervals to determine the levels of inulin, sodium, potassium and osmolality. We observed a significant increase in urinary flow with a peak effect at 90 min (control (C) = 0.20 ± 0.01 and treated (T) = 0.32 ± 0.01 ml g-1 min-1, P&amp;lt;0.05). At 90 min there was a significant increase in perfusate pressure (C = 129.7 ± 4.81 and T = 175.0 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcystin-LR; Kidney; Perfused kidney.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800008

Cocos nucifera (L.) (Arecaceae): A phytochemical and pharmacological review
Provedor de dados: 56
Autores: Lima,E.B.C.; Sousa,C.N.S.; Meneses,L.N.; Ximenes,N.C.; Santos Júnior,M.A.; Vasconcelos,G.S.; Lima,N.B.C.; Patrocínio,M.C.A.; Macedo,D.; Vasconcelos,S.M.M.
Cocos nucifera (L.) (Arecaceae) is commonly called the “coconut tree” and is the most naturally widespread fruit plant on Earth. Throughout history, humans have used medicinal plants therapeutically, and minerals, plants, and animals have traditionally been the main sources of drugs. The constituents of C. nucifera have some biological effects, such as antihelminthic, anti-inflammatory, antinociceptive, antioxidant, antifungal, antimicrobial, and antitumor activities. Our objective in the present study was to review the phytochemical profile, pharmacological activities, and toxicology of C. nucifera to guide future preclinical and clinical studies using this plant. This systematic review consisted of searches performed using scientific databases such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera (L.); Ethnopharmacology; Pharmacological properties; Biological activity; Review.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100953

Reactivity of anti-thyroid antibodies to thyroglobulin tryptic fragments: comparison of autoimmune and non-autoimmune thyroid diseases
Provedor de dados: 56
Autores: Boechat,L.H.B.; Zollner,R.L.
Studies concerning the antigenicity of thyroglobulin fragments allow the characterization of the epitopes but do not consider the role of heavier antigenic fragments that could result in vivo from the action of endoproteases. Here we assess the relative importance of the fragments obtained from thyroglobulin by limited proteolysis with trypsin and compare by immunoblotting their reactivity to serum from patients with autoimmune (Graves' disease and Hashimoto's thyroiditis) and non-autoimmune (subacute thyroiditis) disease. The results showed no difference in frequency of recognition of any peptide by sera from patients with autoimmune thyroiditis. In contrast, sera from patients with subacute thyroiditis reacted more frequently with a peptide of 80 kDa....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoantibodies; Autoimmunity; Thyroglobulin; Trypsin; Thyroiditis; Proteases.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400012

Effects of bromocriptine on serum prolactin levels, pituitary weight and immunoreactive prolactin cells in estradiol-treated ovariectomized rats: an experimental model of estrogen-dependent hyperprolactinemia
Provedor de dados: 56
The present study was designed to assess the effects of bromocriptine, a dopamine agonist, on pituitary wet weight, number of immunoreactive prolactin cells and serum prolactin concentrations in estradiol-treated rats. Ovariectomized Wistar rats were injected subcutaneously with sunflower oil vehicle or estradiol valerate (50 or 300 µg rat-1 week-1) for 2, 4 or 10 weeks. Bromocriptine (0.2 or 0.6 mg rat-1 day-1) was injected daily during the last 5 or 12 days of estrogen treatment. Data were compared with those obtained for intact control rats. Administration of both doses of estrogen increased serum prolactin levels. No difference in the number of prolactin cells in rats treated with 50 µg estradiol valerate was observed compared to intact adult animals....
Palavras-chave: Prolactin levels; Pituitary growth; Immunoreactive prolactin cells; Bromocriptine; Estrogen.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100017

Drag reduction by polyethylene glycol in the tail arterial bed of normotensive and hypertensive rats
Provedor de dados: 56
Autores: Bessa,K.L.; Belletati,J.F.; Dos Santos,L.; Rossoni,L.V.; Ortiz,J.P.
This study was designed to evaluate the effect of drag reducer polymers (DRP) on arteries from normotensive (Wistar) and spontaneously hypertensive rats (SHR). Polyethylene glycol (PEG 4000 at 5000 ppm) was perfused in the tail arterial bed with (E+) and without endothelium (E-) from male, adult Wistar (N = 14) and SHR (N = 13) animals under basal conditions (constant flow at 2.5 mL/min). In these preparations, flow-pressure curves (1.5 to 10 mL/min) were constructed before and 1 h after PEG 4000 perfusion. Afterwards, the tail arterial bed was fixed and the internal diameters of the arteries were then measured by microscopy and drag reduction was assessed based on the values of wall shear stress (WSS) by computational simulation. In Wistar and SHR groups,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shear stress; Flow-pressure response; Drag reduction; Endothelium; Polyethylene glycol; Hypertension.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800006

HLA-DRB1 alleles in juvenile-onset systemic lupus erythematosus: renal histologic class correlations
Provedor de dados: 56
Autores: Liphaus,B.L.; Kiss,M.H.B.; Goldberg,A.C.
Human leukocyte antigens (HLA) DRB1*03 and DRB1*02 have been associated with systemic lupus erythematosus (SLE) in Caucasians and black populations. It has been observed that certain HLA alleles show stronger associations with SLE autoantibodies and clinical subsets, although they have rarely been associated with lupus renal histologic class. In the present study, HLA-DRB1 allele correlations with clinical features, autoantibodies and renal histologic class were analyzed in a cohort of racially mixed Brazilian patients with juvenile-onset SLE. HLA-DRB1 typing was carried out by polymerase chain reaction amplification with sequence-specific primers using genomic DNA from 55 children and adolescents fulfilling at least four of the American College of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Human leukocyte antigen; Lupus nephritis; Juvenile-onset lupus; HLA-DRB1 alleles.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400019

Polymorphism in CYP17, GSTM1 and the progesterone receptor genes and its relationship with mammographic density
Provedor de dados: 56
Autores: Chambo,D.; Kemp,C.; Costa,A.M.M.; Souza,N.C.N.; Guerreiro da Silva,I.D.C.
Radiologic breast density is one of the predictive factors for breast cancer and the extent of the density is directly related to postmenopause. However, some patients have dense breasts even during postmenopause. This condition may be explained by the genes that codify for the proteins involved in the biosynthesis, as well as the activity and metabolism of steroid hormones. They are polymorphic, which could explain the variations of individual hormones and, consequently, breast density. The constant need to find markers that may assist in the primary prevention of breast cancer as well as in selecting high risk patients motived this study. We determined the influence of genetic polymorphism of CYP17 (cytochrome P450c17, the gene involved in steroid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mammography; 17-alpha hydroxylase steroid; Glutathione transferase; Progesterone receptor; Genetic polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400003

Erratum for: “Participation of hepatic α/β-adrenoceptors and AT1 receptors in glucose release and portal hypertensive response induced by adrenaline or angiotensin II” [Braz J Med Biol Res (2018) 51(12): e7526]
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200901

Effect of heat and moisture exchangers on the prevention of ventilator-associated pneumonia in critically ill patients
Provedor de dados: 56
Autores: Auxiliadora-Martins,M.; Menegueti,M.G.; Nicolini,E.A.; Alkmim-Teixeira,G.C.; Bellissimo-Rodrigues,F.; Martins-Filho,O.A.; Basile-Filho,A.
Ventilator-associated pneumonia (VAP) remains one of the major causes of infection in the intensive care unit (ICU) and is associated with the length of hospital stay, duration of mechanical ventilation, and use of broad-spectrum antibiotics. We compared the frequency of VAP 10 months prior to (pre-intervention group) and 13 months after (post-intervention group) initiation of the use of a heat and moisture exchanger (HME) filter. This is a study with prospective before-and-after design performed in the ICU in a tertiary university hospital. Three hundred and fourteen patients were admitted to the ICU under mechanical ventilation, 168 of whom were included in group HH (heated humidifier) and 146 in group HME. The frequency of VAP per 1000 ventilator-days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat and moisture exchanger; Ventilator-associated pneumonia; Quality improvement; Nosocomial infection; Critically ill patients.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200027

Gap effect and reaction time distribution: simple vs choice manual responses
Provedor de dados: 56
Autores: Machado-Pinheiro,W.; Gawryszewski,L.G.; Ribeiro-do-Valle,L.E.
It is well known that saccadic reaction times (SRT) are reduced when the target is preceded by the offset of the fixation point (FP) - the gap effect. Some authors have proposed that the FP offset also allows the saccadic system to generate a separate population of SRT, the express saccades. Nevertheless, there is no agreement as to whether the gap effect and express responses are also present for manual reaction times (MRT). We tested the gap effect and the MRT distribution in two different conditions, i.e., simple and choice MRT. In the choice MRT condition, subjects need to identify the side of the stimulus and to select the appropriate response, while in the simple MRT these stages are not necessary. We report that the gap effect was present in both...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Attention; Reaction time; Gap effect; Vision; Express responses.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000012

High expression of human carboxypeptidase M in Pichia pastoris: Purification and partial characterization
Provedor de dados: 56
Autores: Craveiro,R.B.; Ramalho,J.D.; Chagas,J.R.; Wang,P.H.M.; Casarini,D.E.; Pesquero,J.L.; Araújo,R.C.; Pesquero,J.B.
Carboxypeptidase M (CPM) is an extracellular glycosylphosphatidyl-inositol-anchored membrane glycoprotein, which removes the C-terminal basic residues, lysine and arginine, from peptides and proteins at neutral pH. CPM plays an important role in the control of peptide hormones and growth factor activity on the cell surface. The present study was carried out to clone and express human CPM in the yeast Pichia pastoris in order to evaluate the importance of this enzyme in physiological and pathological processes. The cDNA for the enzyme was amplified from total placental RNA by RT-PCR and cloned in the vector pPIC9, which uses the methanol oxidase promoter and drives the expression of high levels of heterologous proteins in P. pastoris. The cpm gene, after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinins; Human carboxypeptidase M; Recombinant protein; Pichia pastoris.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200007

Analysis of trophic responses in lesioned brain: focus on basic fibroblast growth factor mechanisms
Provedor de dados: 56
Autores: Chadi,G.; Fuxe,K.
The actions of fibroblast growth factors (FGFs), particularly the basic form (bFGF), have been described in a large number of cells and include mitogenicity, angiogenicity and wound repair. The present review discusses the presence of the bFGF protein and messenger RNA as well as the presence of the FGF receptor messenger RNA in the rodent brain by means of semiquantitative radioactive in situ hybridization in combination with immunohistochemistry. Chemical and mechanical injuries to the brain trigger a reduction in neurotransmitter synthesis and neuronal death which are accompanied by astroglial reaction. The altered synthesis of bFGF following brain lesions or stimulation was analyzed. Lesions of the central nervous system trigger bFGF gene expression by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioautography; In situ hybridization; Basic fibroblast growth factor; FGF receptor; Astrocyte; Image analysis; Central nervous system; Brain lesion.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200007

Exogenous normal lymph alleviates microcirculation disturbances and abnormal hemorheological properties in rats with disseminated intravascular coagulation
Provedor de dados: 56
Autores: Niu,Chun-Yu; Zhao,Zi-Gang; Zhang,Yu-Ping; Hou,Ya-Li; Li,Jun-Jie; Jiang,Hua; Zhang,Jing.
Disturbances of the microcirculation and abnormal hemorheological properties are important factors that play an important role in disseminated intravascular coagulation (DIC) and result in organ dysfunction or failure. In the present study, we established an animal model of DIC using intravenous Dextran 500 in rats, and used exogenous normal lymph corresponding to 1/15 of whole blood volume for injection through the left jugular vein. We found that normal lymph could improve the blood pressure and survival time of rats with DIC. The results regarding the mesenteric microcirculation showed that the abnormality of the diameter of mesenteric microvessels and micro-blood flow speed in the DIC+lymph group was significantly less than in the DIC+saline group....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disseminated intravascular coagulation; Lymph; Mesenteric microcirculation; Hemorheology; Dextran 500.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200138

Corticosteroids in acute respiratory distress syndrome
Provedor de dados: 56
Autores: Fernandes,A.B.S.; Zin,W.A.; Rocco,P.R.M.
Improving the course and outcome of patients with acute respiratory distress syndrome presents a challenge. By understanding the immune status of a patient, physicians can consider manipulating proinflammatory systems more rationally. In this context, corticosteroids could be a therapeutic tool in the armamentarium against acute respiratory distress syndrome. Corticosteroid therapy has been studied in three situations: prevention in high-risk patients, early treatment with high-dose, short-course therapy, and prolonged therapy in unresolving cases. There are differences between the corticosteroid trials of the past and recent trials: today, treatment starts 2-10 days after disease onset in patients that failed to improve; in the past, the corticosteroid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute respiratory distress syndrome; Collagen; Cytokine; Methylprednisolone; Experimental models; Clinical trials.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200003

MicroRNA-340-5p modulates cisplatin resistance by targeting LPAATβ in osteosarcoma
Provedor de dados: 56
Autores: Song,L.; Duan,P.; Gan,Y.; Li,P.; Zhao,C.; Xu,J.; Zhang,Z.; Zhou,Q.
MicroRNAs (miRNAs) play an important role in drug resistance and modulate the efficiency of chemotherapy. A recent study indicated that miR-340 functions as a tumor suppressor in various types of cancer. However, the role of miR-340 in chemotherapy has not been reported yet. In this study, we found that miR-340 enhanced cisplatin (CDDP)-induced cell death. Induction of miR-340-5p expression decreased the IC50 of CDDP and increased the apoptosis of CDDP-resistant MG-63 and Saos-2 cells. Moreover, miR-340-5p decreased the accumulation of MRP1 and MDR1. We further explored the mechanism underlying the promoting effects of miR-340-5p on CDDP-induced cell death. We identified a potential target of miR-340 in the 3′ untranslated region of lysophosphatidic acid...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Sensitivity to cisplatin; LPAATβ; MiR-340-5p; Osteosarcoma.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500606

Carotid intima-media thickness and plaques in internal carotid artery as surrogate markers of lower limb arterial lesions in Chinese patients with diabetic foot
Provedor de dados: 56
Autores: Zhang,Mei; Wen,Xiaorong; Zhou,Chenyun; Huang,Jing; He,Ying.
Peripheral arterial disease (PAD) can impair healing of diabetic foot (DF) in patients with diabetes mellitus. To determine whether carotid intima-media thickness (CIMT) can predict lower limb arterial lesions in patients with DF, this cross-sectional study enrolled patients with DF at West China Hospital (China) between January 2012 and December 2015. Ultrasonography was used to measure CIMT, assess the internal carotid arteries (ICA) for plaques, and evaluate lower limb segmental arteries for stenosis. The optimal CIMT cutoff for detecting lower limb PAD was determined by receiver operating characteristic (ROC) curve analysis. Factors associated with PAD were identified by logistic regression analyses. A total of 167 patients (mean age: 69.7±10.3 years;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Diabetic foot; Carotid artery; Intima-media thickness; Plaque; Peripheral arterial disease.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700611

Activation of neural cholecystokinin-1 receptors induces relaxation of the isolated rat duodenum which is reduced by nitric oxide synthase inhibitors
Provedor de dados: 56
Autores: Martins,S.R.; de Oliveira,R.B.; Ballejo,G.
Cholecystokinin (CCK) influences gastrointestinal motility, by acting on central and peripheral receptors. The aim of the present study was to determine whether CCK has any effect on isolated duodenum longitudinal muscle activity and to characterize the mechanisms involved. Isolated segments of the rat proximal duodenum were mounted for the recording of isometric contractions of longitudinal muscle in the presence of atropine and guanethidine. CCK-8S (EC50: 39; 95% CI: 4.1-152 nM) and cerulein (EC50: 58; 95% CI: 18-281 nM) induced a concentration-dependent and tetrodotoxin-sensitive relaxation. Nomeganitro-L-arginine (L-NOARG) reduced CCK-8S- and cerulein-induced relaxation (IC50: 5.2; 95% CI: 2.5-18 µM) in a concentration-dependent manner. The magnitude...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCK; CCK receptor subtype 1; Proximal duodenum; Myenteric neurons; Nitric oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200014

Effect of pre-exercise carbohydrate availability on fat oxidation and energy expenditure after a high-intensity exercise
Provedor de dados: 56
Autores: Ferreira,G.A.; Felippe,L.C.; Silva,R.L.S.; Bertuzzi,R.; De Oliveira,F.R.; Pires,F.O.; Lima-Silva,A.E.
The aim of this study was to test the hypothesis that reduced pre-exercise carbohydrate (CHO) availability potentiates fat oxidation after an exhaustive high-intensity exercise bout. Eight physically active men underwent a high-intensity exercise (∼95% V̇O2max) until exhaustion under low or high pre-exercise CHO availability. The protocol to manipulate pre-exercise CHO availability consisted of a 90-min cycling bout at ∼70% V̇O2max + 6 × 1-min at 125% V̇O2max with 1-min rest, followed by 48 h under a low- (10% CHO, low-CHO availability) or high-CHO diet (80% CHO, high-CHO availability). Time to exhaustion was shorter and energy expenditure (EE) lower during the high-intensity exercise in low- compared to high-CHO availability (8.6±0.8 and 11.4±1.6 min, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Excess post-exercise oxygen consumption; Fat oxidation; Carbohydrate oxidation; Diet manipulation; Aerobic metabolism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500617

Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King &amp;amp; H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro
Provedor de dados: 56
Autores: Almeida,V.G.; Avelar-Freitas,B.A.; Santos,M.G.; Costa,L.A.; Silva,T.J.; Pereira,W.F.; Amorim,M.L.L.; Grael,C.F.F.; Gregório,L.E.; Rocha-Vieira,E.; Brito-Melo,G.E.A.
Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Lymphocyte proliferation; Luteolin; Medicinal plant; Pseudobrickellia brasiliensis; Quinic acid.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800603

Cytokine profile and nitric oxide levels in sera from patients with brucellosis
Provedor de dados: 56
Autores: Refik,M.; Mehmet,N.; Durmaz,R.; Ersoy,Y.
The aims of this study were to investigate the serum levels of some cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin 1ß (IL-1ß), IL-2R, IL-6, and IL-8] and nitric oxide (NO) levels in patients with untreated brucellosis and to test the correlation of these parameters with each other. The study was conducted on 67 subjects, 37 patients with brucellosis and 30 healthy individuals with no history of Brucella infection. Brucellosis was identified by a positive blood culture and/or increased Brucella antibodies in serological tests in addition to compatible clinical symptoms. Cytokine profile analysis was performed by the immulite chemiluminescent enzyme immunometric assay whose inter- and intra-assay coefficients of variance were 2.6-3.6 and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human brucellosis; Cytokines; Nitric oxide; Interleukin-2; Interleukin-6; Interleukin-8.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100010

Gender differences in vascular expression of endothelin and ET A/ET B receptors, but not in calcium handling mechanisms, in deoxycorticosterone acetate-salt hypertension
Provedor de dados: 56
Autores: David,F.L.; Montezano,A.C.I.; Rebouças,N.A.; Nigro,D.; Fortes,Z.B.; Carvalho,M.H.C.; Tostes,R.C.A.
We determined if the increased vascular responsiveness to endothelin-1 (ET-1) observed in male, but not in female, DOCA-salt rats is associated with differential vascular mRNA expression of ET-1 and/or ET A/ET B receptors or with functional differences in Ca2+ handling mechanisms by vascular myocytes. Uninephrectomized male and female Wistar rats received DOCA and drinking water containing NaCl/KCl. Control rats received vehicle and tap water. Blood pressure and contractile responses of endothelium-denuded aortic rings to agents which induce Ca2+ influx and/or its release from internal stores were measured using standard procedures. Expression of mRNA for ET-1 and ET A/ET B receptors was evaluated by RT-PCR after isolation of total cell RNA from both aorta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DOCA-salt hypertension; Endothelin-1; Gender; Endothelin receptors; Calcium; Rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900006

Arachidonic acid triggers an oxidative burst in leukocytes
Provedor de dados: 56
Autores: Pompeia,C.; Cury-Boaventura,M.F.; Curi,R.
The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachidonic acid; NADPH oxidase; Nitroblue tetrazolium; Leukocytes; Reactive oxygen species.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013

Role of bradykinin in postprandial hypotension in humans
Provedor de dados: 56
Autores: Tumilero,S.; Gallo Jr.,L.; Foss,M.C.; Gouveia,L.M.F.; Reis,M.L.; Rothschild,A.M.
A transient significant decrease in mean arterial blood pressure (MAP) from 107 ± 3 to 98 ± 3 mmHg (P&amp;lt;0.05) was observed in elderly (59-69 years of age), healthy volunteers 25-30 min following ingestion of a test meal. In young volunteers (22-34 years of age), a postprandial decrease of MAP from 88 ± 3 to 83 ± 4 mmHg was also noted but it was not statistically significant. A 40% decrease in bradykinin (BK) content of circulatory high molecular weight kininogen had previously been observed in human subjects given the same test meal. We presently demonstrate by specific ELISA that the stable pentapeptide metabolite (1-5 BK) of BK increases from 2.5 ± 1.0 to 11.0 ± 2.5 pg/ml plasma (P&amp;lt;0.05) in elderly volunteers and from 2.0 ± 1.0 to 10.3 ± 3.2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradykinin; Postprandial hypotension; Indocyanine green; Captopril; 1-5 bradykinin.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600015

Hydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains
Provedor de dados: 56
Autores: Silveira,F.Q.P.; Ximenes,F.A.; Cacais,A.O.G.; Milagres,A.M.F.; Medeiros,C.L.; Puls,J.; Filho,E.X.F.
Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28oC in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylobiose and xylotriose to higher xylooligosaccharides. A purified ß-xylosidase from the Trichoderma harzianum strain released xylose, xylobiose and xylotriose from seaweed, deacetylated, oat spelt and birchwood xylans. The purified enzyme was not active against acetylated xylan and catalyzed the hydrolysis of xylooligosaccharides, including xylotriose, xylotetraose and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Trichoderma; Xylan; Xylanase.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800003

Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
Provedor de dados: 56
Autores: Tanan,C.L.; Ventura,D.F.; de Souza,J.M.; Grotzner,S.R.; Mela,M.; Gouveia Jr.,A.; Oliveira-Ribeiro,C.A.
Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methyl mercury; Horizontal cells; Electrophysiology; Hoplias malabaricus; Retina.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700017

Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems
Provedor de dados: 56
Autores: De Angelis,K.; Gama,V.M.; Farah,V.A.M.; Irigoyen,M.C.
The use of colored microspheres to adequately evaluate blood flow changes under different circumstances in the same rat has been validated with a maximum of three different colors due to methodological limitations. The aim of the present study was to validate the use of four different colors measuring four repeated blood flow changes in the same rat to assess the role of vasopressor systems in controlling arterial pressure (AP). Red (150,000), white (200,000), yellow (150,000), and blue (200,000) colored microspheres were infused into the left ventricle of 6 male Wistar rats 1) at rest and 2) after vasopressin (aAVP, 10 µg/kg, iv), 3) renin-angiotensin (losartan, 10 mg/kg, iv), and 4) sympathetic system blockade (hexamethonium, 20 mg/kg, iv) to determine...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Colored microspheres; Arterial pressure; Sympathetic system; Renin-angiotensin; Vasopressin; Local blood flow measurement.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100018

The 42-kDa coat protein of Andean potato mottle virus acts as a transcriptional activator in yeast
Provedor de dados: 56
Autores: Vidal,M.S.; Margis,R.
Interactions of viral proteins play an important role in the virus life cycle, especially in capsid assembly. Andean potato mottle comovirus (APMoV) is a plant RNA virus with a virion formed by two coat proteins (CP42 and CP22). Both APMoV coat protein open reading frames were cloned into pGBT9 and pGAD10, two-hybrid system vectors. HF7c yeast cells transformed with the p9CP42 construct grew on yeast dropout selection media lacking tryptophan and histidine. Clones also exhibited ß-galactosidase activity in both qualitative and quantitative assays. These results suggest that CP42 protein contains an amino acid motif able to activate transcription of His3 and lacZ reporter genes in Saccharomyces cerevisiae. Several deletions of the CP42 gene were cloned into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Andean potato mottle virus; Virus assembly; Coat protein; Transactivation; Yeast two-hybrid system.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400001

Morphological and functional alterations of the intestine of rats with iron-deficiency anemia
Provedor de dados: 56
Autores: Wayhs,M.L.C.; Patrício,F.S.R.; Amancio,O.M.S.; Pedroso,M.Z.; Fagundes Neto,U.; Morais,M.B.
The present study was designed to assess the intestinal absorption of D-xylose and jejunal morphometry in rats with iron-deficiency anemia. Male Wistar rats were randomly divided into a control group (diet containing 50 mg Fe/kg, N = 12) and an anemic group (diet containing &lt;5 mg Fe/kg, N = 12). The animals were housed in individual metabolic cages and deionized water and diet were provided ad libitum for 6 weeks. Hemoglobin and hematocrit were determined at 0, 2, 4, and 6 weeks. At the end of the study the rats were submitted to a D-xylose absorption test (50 mg/100 g body weight) and sacrificed and a jejunal specimen was obtained for morphometric study. At the end of the study the hemoglobin and hematocrit of the anemic rats (8.7 ± 0.9 g/dl and 34.1 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron deficiency; Anemia; Jejunal villus height; Jejunal mucosal thickness; Jejunal epithelial surface.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100006

Temperature, air pollution, and mortality from myocardial infarction in São Paulo, Brazil
Provedor de dados: 56
Autores: Sharovsky,R.; César,L.A.M.; Ramires,J.A.F.
An increase in daily mortality from myocardial infarction has been observed in association with meteorological factors and air pollution in several cities in the world, mainly in the northern hemisphere. The objective of the present study was to analyze the independent effects of environmental variables on daily counts of death from myocardial infarction in a subtropical region in South America. We used the robust Poisson regression to investigate associations between weather (temperature, humidity and barometric pressure), air pollution (sulfur dioxide, carbon monoxide, and inhalable particulate), and the daily death counts attributed to myocardial infarction in the city of São Paulo in Brazil, where 12,007 fatal events were observed from 1996 to 1998....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Myocardial infarction; Mortality; Temperature; Atmospheric pressure; Air pollution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100009

Fetal development of regulatory mechanisms for body fluid homeostasis
Provedor de dados: 56
Autores: Guan,J.; Mao,C.; Feng,X.; Zhang,H.; Xu,F.; Geng,C.; Zhu,L.; Wang,A.; Xu,Z.
The balance of body fluids is critical to health and the development of diseases. Although quite a few review papers have shown that several mechanisms, including hormonal and behavioral regulation, play an important role in body fluid homeostasis in adults, there is limited information on the development of regulatory mechanisms for fetal body fluid balance. Hormonal, renal, and behavioral control of body fluids function to some extent in utero. Hormonal mechanisms including the renin-angiotensin system, aldosterone, and vasopressin are involved in modifying fetal renal excretion, reabsorption of sodium and water, and regulation of vascular volume. In utero behavioral changes, such as fetal swallowing, have been suggested to be early functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fetal body fluids; Fetal hormonal regulation; Fetal renal regulation; Swallowing.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600002

Proteomic data show an increase in autoantibodies and alpha-fetoprotein and a decrease in apolipoprotein A-II with time in sera from senescence-accelerated mice
Provedor de dados: 56
Autores: Guo,S.J.; Qi,C.H.; Zhou,W.X.; Zhang,Y.X.; Zhang,X.M.; Wang,J.; Wang,H.X.
We evaluated changes in levels by comparing serum proteins in senescence-accelerated mouse-prone 8 (SAMP8) mice at 2, 6, 12, and 15 months of age (SAMP8-2 m, -6 m, -12 m, -15 m) to age-matched SAM-resistant 1 (SAMR1) mice. Mice were sacrificed, and blood was analyzed by 2-dimensional electrophoresis combined with mass spectrometry. Five protein spots were present in all SAMP8 serum samples, but only appeared in SAMR1 samples at 15 months of age except for spot 3, which also showed a slight expression in SAMR1-12 m sera. Two proteins decreased in the sera from SAMP8-2 m, -6 m, and -12 m mice, and divided into 2 spots each in SAMP8-15 m sera. Thus, the total number of altered spots in SAMP8 sera was 7; of these, 4 were identified as Ig kappa chain V region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SAMP8; Serum proteins; 2-DE; Mass spectrometry; M-T413.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500417

Renal and extrarenal manifestations of autosomal dominant polycystic kidney disease
Provedor de dados: 56
Autores: Romão,E.A.; Moysés Neto,M.; Teixeira,S.R.; Muglia,V.F.; Vieira-Neto,O.M.; Dantas,M.
The objective of the present study was to determine the frequency of the most common clinical features in patients with autosomal dominant polycystic kidney disease in a sample of the Brazilian population. The medical records of 92 patients with autosomal dominant polycystic kidney disease attended during the period from 1985 to 2003 were reviewed. The following data were recorded: age at diagnosis, gender, associated clinical manifestations, occurrence of stroke, age at loss of renal function (beginning of dialysis), and presence of a family history. The involvement of abdominal viscera was investigated by ultrasonography. Intracranial alterations were prospectively investigated by magnetic resonance angiography in 42 asymptomatic patients, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autosomal dominant polycystic kidney disease; Hepatic cyst; Intracranial aneurysm; Arachnoid cyst; End-stage renal disease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400014

A functional study of caustic strictures of the esophagus in children
Provedor de dados: 56
Autores: Da-Costa-Pinto,E.A.L.; Dorsa,T.K.; Altimani,A.; Andreollo,N.A.; Cardoso,S.R.; Morais,D.J.; Bustorff-Silva,J.M.
The objective of the present study was to assess esophageal motor function in 21 children (7.5 ± 2.9 years) with caustic strictures. Esophageal manometry was performed using a water-infusion system interfaced with a polygraph and displayed on a computer screen. The data were compared with those obtained from 9 healthy children. Radionuclide transit was determined by studying deglutition of a single bolus of 99mTc pertechnetate in 10 ml of water. Non-peristaltic low-amplitude and long-duration waves were the most common findings detected in patients with strictures longer than 20% of esophageal length (N = 11). Compared with the control group, these patients presented lower mean amplitude and longer mean duration of waves (24.4 ± 11.2 vs 97.9 ± 23.7 mmHg, P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caustic esophagitis; Esophageal dysfunction; Manometry; Esophageal transit.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100005

The role of secondary hyperparathyroidism in left ventricular hypertrophy of patients under chronic hemodialysis
Provedor de dados: 56
Autores: Randon,R.B.; Rohde,L.E.; Comerlato,L.; Ribeiro,J.P.; Manfro,R.C.
End-stage renal disease (ESRD) patients frequently develop structural cardiac abnormalities, particularly left ventricular hypertrophy (LVH). The mechanisms involved in these processes are not completely understood. In the present study, we evaluated a possible association between parathyroid hormone (PTH) levels and left ventricular mass (LVM) in patients with ESRD. Stable uremic patients on intermittent hemodialysis treatment were evaluated by standard two-dimensional echocardiography and their sera were analyzed for intact PTH. Forty-one patients (mean age 45 years, range 18 to 61 years), 61% males, who had been on hemodialysis for 3 to 186 months, were evaluated. Patients were stratified into 3 groups according to serum PTH: low levels (&lt;100 pg/ml;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: End-stage renal failure; Parathyroid hormone; Echocardiography; Left ventricular hypertrophy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900016

Effects of sedation during upper gastrointestinal endoscopy on endocrine response and cardiorespiratory function
Provedor de dados: 56
Autores: Yetkin,G.; Oba,S.; Uludag,M.; Paksoy,I.; Akgün,I.; Eren,N.
Upper gastrointestinal endoscopy is often accompanied by tachycardia which is known to be an important pathogenic factor in the development of myocardial ischemia. The pathogenesis of tachycardia is unknown but the condition is thought to be due to the endocrine response to endoscopy. The purpose of the present study was to investigate the effects of sedation on the endocrine response and cardiorespiratory function. Forty patients scheduled for diagnostic upper gastrointestinal endoscopy were randomized into 2 groups. While the patients in the first group did not receive sedation during upper gastrointestinal endoscopy, the patients in the second group were sedated with intravenous midazolam at the dose of 5 mg for those under 65 years or 2.5 mg for those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress response; Oxygen saturation; Sedation; Upper gastrointestinal endoscopy; Tachycardia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200008

A DNA enzyme targeting Egr-1 inhibits rat vascular smooth muscle cell proliferation by down-regulation of cyclin D1 and TGF-β1
Provedor de dados: 56
Autores: Wu,Y.; Han,W.; Liu,G.-N.
We have demonstrated that a synthetic DNA enzyme targeting early growth response factor-1 (Egr-1) can inhibit neointimal hyperplasia following vascular injury. However, the detailed mechanism of this inhibition is not known. Thus, the objective of the present study was to further investigate potential inhibitory mechanisms. Catalytic DNA (ED5) and scrambled control DNA enzyme (ED5SCR) were synthesized and transfected into primary cultures of rat vascular smooth muscle cells (VSMCs). VSMC proliferation and DNA synthesis were analyzed by the MTT method and BrdU staining, respectively. Egr-1, TGF-β1, p53, p21, Bax, and cyclin D1 expression was detected by RT-PCR and Western blot. Apoptosis and cell cycle assays were performed by FACS. Green fluorescence could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA enzyme; Egr-1; VSMC; Proliferation; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100004

Naringenin regulates production of matrix metalloproteinases in the knee-joint and primary cultured articular chondrocytes and alleviates pain in rat osteoarthritis model
Provedor de dados: 56
Autores: Wang,C.C.; Guo,L.; Tian,F.D.; An,N.; Luo,L.; Hao,R.H.; Wang,B.; Zhou,Z.H.
Inflammation of cartilage is a primary symptom for knee-joint osteoarthritis. Matrix metalloproteinases (MMPs) are known to play an important role in the articular cartilage destruction related to osteoarthritis. Naringenin is a plant-derived flavonoid known for its anti-inflammatory properties. We studied the effect of naringenin on the transcriptional expression, secretion and enzymatic activity of MMP-3 in vivo in the murine monosodium iodoacetate (MIA) osteoarthritis model. The assessment of pain behavior was also performed in the MIA rats. The destruction of knee-joint tissues was analyzed microscopically. Moreover, the effect of naringenin was also studied in vitro in IL-1β activated articular chondrocytes. The transcriptional expression of MMP-3,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metalloproteinase; Osteoarthritis; Naringenin; Chondrocyte; Knee-joint; Rat MIA model.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400603

Tissue-specific regulation of IRS-1 in unilaterally nephrectomized rats
Provedor de dados: 56
Autores: Sasse,A.D.; Chen,E.; Carvalho,C.R.O.; Gontijo,J.A.R.; Brenelli,S.L.; Saad,M.J.A.
Insulin stimulates the tyrosine kinase activity of its receptor, resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate 1 (IRS-1). IRS-1 is also a substrate for different peptides and growth factors, and a transgenic mouse "knockout" for this protein does not have normal growth. However, the role of IRS-1 in kidney hypertrophy and/or hyperplasia was not investigated. In the present study we investigated IRS-1 protein and tyrosine phosphorylation levels in the remnant kidney after unilateral nephrectomy (UNX) in 6-week-old male Wistar rats. After insulin stimulation the levels of insulin receptor and IRS-1 tyrosine phosphorylation were reduced to 79 ± 5% (P&amp;lt;0.005) and 58 ± 6% (P&amp;lt;0.0001), respectively, of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin receptor; Insulin receptor substrate; Unilateral nephrectomy; Insulin action.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000004

Computed tomography findings of hepatic veno-occlusive disease caused by Sedum aizoon with histopathological correlation
Provedor de dados: 56
Autores: Shao,H.; Chen,H. Z.; Zhu,J. S.; Ruan,B.; Zhang,Z. Q.; Lin,X.; Gan,M. F.
This study investigated the value of computed tomography (CT) in the diagnosis and treatment of hepatic veno-occlusive disease (HVOD) caused by Sedum aizoon (SA). The clinical manifestations, treatment results, imaging findings, and histological findings of the liver were analyzed in 39 patients with HVOD caused by SA. Hepatomegaly, liver dysfunction, abdominal effusion, and geographic density changes on liver CT scans were found in all 39 patients. The pathological findings of histological liver examination included swelling and point-like necrosis of liver cells, significant expansion and congestion of the sinuses, endothelial swelling, and wall thickening with incomplete lumen occlusion of small liver vessels. CT geographic density changes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic veno-occlusive disease; Sedum aizoon; Drug-induced liver disease; Computed tomography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201145

The role of oxytocin in cardiovascular regulation
Provedor de dados: 56
Autores: Gutkowska,J.; Jankowski,M.; Antunes-Rodrigues,J.
Studies of body volume expansion have indicated that lesions of the anteroventral third ventricle and median eminence block the release of atrial natriuretic peptide (ANP) into the circulation. Detailed analysis of the lesions showed that activation of oxytocin (OT)-ergic neurons is responsible for ANP release, and it has become clear that activation of neuronal circuitry elicits OT secretion into the circulation, activating atrial OT receptors and ANP release from the heart. Subsequently, we have uncovered the entire functional OT system in the rat and the human heart. An abundance of OT has been observed in the early development of the fetal heart, and the capacity of OT to generate cardiomyocytes (CMs) has been demonstrated in various types of stem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxytocin; Natriuretic peptides; Heart; Cardiomyogenesis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300206

Effects of pentoxifylline treatment before freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method
Provedor de dados: 56
Autores: Esteves,S.C.; Spaine,D.M.; Cedenho,A.P.
The objective of the present study was to investigate the effects of the direct addition of pentoxifylline (PF) to the ejaculates of men with poor sperm quality before freezing on post-thaw sperm motility, viability, acrosome integrity, and agonist-induced acrosome reaction. Semen specimens from 16 infertile men with impaired sperm count and motility (oligoasthenozoospermia) were divided into two equal aliquots: one received no treatment (control) while the other was incubated with 5 mM PF (treated). Both aliquots were cryopreserved by the liquid nitrogen vapor method. Motility was assessed according to WHO criteria. Acrosome integrity and spontaneous and calcium ionophore-induced acrosome reactions were assessed with fluorescein isothiocyanate-conjugated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrosome; Cryopreservation; Human spermatozoa; Ionophore; Pentoxifylline; Oligoasthenozoospermia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700014

Idiopathic focal segmental glomerulosclerosis and HLA antigens
Provedor de dados: 56
Autores: Gerbase-DeLima,M.; Pereira-Santos,A.; Sesso,R.; Temin,J.; Aragão,E.S.; Ajzen,H.
The objective of the present study was to investigate a possible association between HLA class II antigens and idiopathic focal segmental glomerulosclerosis (FSGS). HLA-A, -B, -DR and -DQ antigens were determined in 19 Brazilian patients (16 white subjects and three subjects of Japanese origin) with biopsy-proven FSGS. Comparison of the HLA antigen frequencies between white patients and white local controls showed a significant increase in HLA-DR4 frequency among FSGS patients (37.7 vs 17.2%, P&amp;lt;0.05). In addition, the three patients of Japanese extraction, not included in the statistical analysis, also presented HLA-DR4. In conclusion, our data confirm the association of FSGS with HLA-DR4 previously reported by others, thus providing further...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Focal segmental glomerulosclerosis; Genetics; HLA antigens; MHC.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300010

Decision support system for the diagnosis of schizophrenia disorders
Provedor de dados: 56
Autores: Razzouk,D.; Mari,J.J.; Shirakawa,I.; Wainer,J.; Sigulem,D.
Clinical decision support systems are useful tools for assisting physicians to diagnose complex illnesses. Schizophrenia is a complex, heterogeneous and incapacitating mental disorder that should be detected as early as possible to avoid a most serious outcome. These artificial intelligence systems might be useful in the early detection of schizophrenia disorder. The objective of the present study was to describe the development of such a clinical decision support system for the diagnosis of schizophrenia spectrum disorders (SADDESQ). The development of this system is described in four stages: knowledge acquisition, knowledge organization, the development of a computer-assisted model, and the evaluation of the system's performance. The knowledge was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinical decision support systems; Artificial intelligence; Decision making; Expert systems; Schizophrenia; Medical informatics.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100014

Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model
Provedor de dados: 56
Autores: Li,N.Q.; Yang,J.; Cui,L.; Ma,N.; Zhang,L.; Hao,L.R.
The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA miR-483 Renal fibrosis Igf2; UUO.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600486

Protein dynamics: hydration and cavities
Provedor de dados: 56
Autores: Heremans,K.
The temperature-pressure behavior of proteins seems to be unique among the biological macromolecules. Thermodynamic as well as kinetic data show the typical elliptical stability diagram. This may be extended by assuming that the unfolded state gives rise to volume and enthalpy-driven liquid-liquid transitions. A molecular interpretation follows from the temperature and the pressure dependence of the hydration and cavities. We suggest that positron annihilation spectroscopy can provide additional quantitative evidence for the contributions of cavities to the dynamics of proteins. Only mature amyloid fibrils that form from unfolded proteins are very resistant to pressure treatment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stability diagram; Thermodynamics; High pressure; Unfolding; Amyloid fibrils; Protein dynamics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800002

Correlation between serum 25-hydroxyvitamin D levels and carotid intima-media thickness in a Brazilian population descended from African slaves
Provedor de dados: 56
Autores: Monteiro Júnior,F.C.; Mandarino,N.R.; Santos,E.M.; Santos,A.M.; Salgado,J.V.; Brito,D.J.A.; Salgado,B.J.L.; Lages,J.S.; Castelo Branco,G.; Salgado Filho,N.
Hypovitaminosis D has been identified as a possible new cardiovascular risk factor. However, the results of studies correlating serum vitamin D levels with markers of subclinical atherosclerosis have been conflicting. The aim of this study was to correlate serum levels of 25-hydroxyvitamin D [25(OH)D] with carotid intima-media thickness (C-IMT) and conventional cardiovascular risk factors in Afro-descendants. A cross-sectional analysis was performed on a sample of 382 individuals from a cohort of descendants of African slaves, inhabitants of “Quilombola” communities, with a mean age of 57.79 ±15.3 years, 54.5% of whom were women. Socio-demographic and clinical data were collected and biochemical tests were performed, including serum levels of 25(OH)D by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin D deficiency; Carotid intima-media thickness (C-IMT); Atherosclerosis; African continental ancestry group.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400606

Bovine serum albumin potentiates caffeine- or ATP-induced tension in human skinned skeletal muscle fibers
Provedor de dados: 56
Human skinned muscle fibers were used to investigate the effects of bovine serum albumin (BSA) on the tension/pCa relationship and on the functional properties of the Ca2+-release channel of the sarcoplasmic reticulum (SR). In both fast- and slow-type fibers, identified by their tension response to pSr 5.0, BSA (0.7-15 µM) had no effect on the Ca2+ affinity of the contractile proteins and elicited no tension per se in Ca2+-loaded fibers. In contrast, BSA (&amp;gt;1.0 µM) potentiated the caffeine-induced tension in Ca2+-loaded fibers, this effect being more intense in slow-type fibers. Thus, BSA reduced the threshold caffeine concentration required for eliciting detectable tension, and increased the amplitude, the rate of rise and the area under the curve...
Palavras-chave: Calcium-release channel; Sarcoplasmic reticulum; Human muscle fiber; Bovine serum albumin; Caffeine.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500017

Effects of propofol on lipopolysaccharide-induced expression and release of HMGB1 in macrophages
Provedor de dados: 56
Autores: Wang,T.; Wei,X.Y.; Liu,B.; Wang,L.J.; Jiang,L.H.
This study aimed to determine the effects of different concentrations of propofol (2,6-diisopropylphenol) on lipopolysaccharide (LPS)-induced expression and release of high-mobility group box 1 protein (HMGB1) in mouse macrophages. Mouse macrophage cell line RAW264.7 cells were randomly divided into 5 treatment groups. Expression levels of HMGB1 mRNA were detected using RT-PCR, and cell culture supernatant HMGB1 protein levels were detected using enzyme-linked immunosorbent assay (ELISA). Translocation of HMGB1 from the nucleus to the cytoplasm in macrophages was observed by Western blotting and activity of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the nucleus was detected using ELISA. HMGB1 mRNA expression levels increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; HMGB1; LPS; RAW 264.7 cells; NF-κB.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400286

Role of 11β-hydroxysteroid dehydrogenase 2 renal activity in potassium homeostasis in rats with chronic renal failure
Provedor de dados: 56
Autores: Yeyati,N.L.; Altuna,M.E.; Damasco,M.C.; Mac Laughlin,M.A.
Aldosterone concentrations vary in advanced chronic renal failure (CRF). The isozyme 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2), which confers aldosterone specificity for mineralocorticoid receptors in distal tubules and collecting ducts, has been reported to be decreased or normal in patients with renal diseases. Our objective was to determine the role of aldosterone and 11β-HSD2 renal microsome activity, normalized for glomerular filtration rate (GFR), in maintaining K+ homeostasis in 5/6 nephrectomized rats. Male Wistar rats weighing 180-220 g at the beginning of the study were used. Rats with experimental CRF obtained by 5/6 nephrectomy (N = 9) and sham rats (N = 10) were maintained for 4 months. Systolic blood pressure and plasma creatinine (Pcr)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aldosterone; 5/6 Nephrectomy; 11β-HSD2; Potassium excretion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100008

Genetic analysis of the ELOVL6 gene polymorphism associated with type 2 diabetes mellitus
Provedor de dados: 56
Autores: Liu,Y.; Wang,F.; Yu,X.L.; Miao,Z.M.; Wang,Z.C.; Chen,Y.; Wang,Y.G.
Recent animal studies have indicated that overexpression of the elongation of long-chain fatty acids family member 6 (Elovl6) gene can cause insulin resistance and β-cell dysfunction. These are the major factors involved in the development of type 2 diabetes mellitus (T2DM). To identify the relationship between single nucleotide polymorphisms (SNP) ofELOVL6 and T2DM pathogenesis, we conducted a case-control study of 610 Han Chinese individuals (328 newly diagnosed T2DM and 282 healthy subjects). Insulin resistance and islet first-phase secretion function were evaluated by assessment of insulin resistance in a homeostasis model (HOMA-IR) and an arginine stimulation test. Three SNPs of the ELOVL6 gene were genotyped with polymerase chain reaction-restriction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elongation of long-chain fatty acids family member 6; Single nucleotide polymorphism; Type 2 diabetes mellitus; Insulin resistance; Β-cell function.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700623

Use of flash glucose-sensing technology in patients with type 2 diabetes treated with liraglutide combined with CSII: a pilot study
Provedor de dados: 56
Autores: Yao,Ming-yan; Li,Li-qin; Ma,Jian-xia; Xue,Peng; Li,Yu-kun.
Glycemic variability (GV) may be linked to the development of diabetic complications by inducing inflammation, oxidative stress, and endothelial dysfunction. Flash glucose monitoring (FGM) provides a novel method of continuously monitoring interstitial glucose levels for up to 14 days. This study randomly assigned poorly controlled type 2 diabetes mellitus patients treated with metformin and multiple daily injections of insulin (n=60) to either continuous subcutaneous insulin infusion (CSII) treatment or CSII in combination with liraglutide (CSII+Lira) treatment for 14 days during hospitalization. GV was assessed using a FGM system; weight and cardiometabolic biomarkers were also evaluated. The coefficient of variation was significantly reduced in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flash glucose monitoring; Glucagon-like peptide-1 analog; Continuous subcutaneous insulin infusion; Glucose fluctuation; Cardiometabolic risk markers; Type 2 diabetes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100604

Adult male circumcision with a circular stapler versus conventional circumcision: A prospective randomized clinical trial
Provedor de dados: 56
Autores: Jin,X.D.; Lu,J.J.; Liu,W.H.; Zhou,J.; Yu,R.K.; Yu,B.; Zhang,X.J.; Shen,B.H.
Male circumcision is the most frequently performed procedure by urologists. Safety and efficacy of the circumcision procedure requires continual improvement. In the present study, we investigated the safety and efficacy of a new male circumcision technique involving the use of a circular stapler. In total, 879 consecutive adult male patients were randomly divided into 2 groups: 441 underwent stapler circumcision, and 438 underwent conventional circumcision. The operative time, pain score, blood loss volume, healing time, treatment costs, and postoperative complications were compared between the two groups. The operative time and blood loss volume were significantly lower in the stapler group than in the conventional group (6.8 ± 3.1 vs 24.2 ± 3.2 min and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circumcision; Complication; Male; Stapler.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600577

The use and misuse of the "impact factor" as a parameter for evaluation of scientific publication quality: a proposal to rationalize its application
Provedor de dados: 56
Autores: Coelho,P.M.Z.; Antunes,C.M.F.; Costa,H.M.A.; Kroon,E.G.; Sousa Lima,M.C.; Linardi,P.M.
We present a critical analysis of the generalized use of the "impact factor". By means of the Kruskal-Wallis test, it was shown that it is not possible to compare distinct disciplines using the impact factor without adjustments. After assigning the median journal the value of one (1.000), the impact factor value for each journal was calculated by the rule of three. The adjusted values were homogeneous, thus permitting comparison among distinct disciplines.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Impact factor; Multidisciplinary adjustment; Citation index; Bibliometric evaluation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200001

Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin
Provedor de dados: 56
Autores: Bracesco,N.; Sosa,V.; Blanc,L.; Contreras,V.; Candreva,E.C.; Salvo,V.A.; Hocart,S.; Mechoso,B.; Nunes,E.
DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiation damage; Ilex paraguariensis infusion; Rutin; Radioprotection; Antimutagenic effects; Saccharomyces cerevisiae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900606

Involvement of dopamine receptors in diethylpropion-induced conditioning place preference
Provedor de dados: 56
Autores: Planeta,C.S.; DeLucia,R.
Diethylpropion (DEP) is an amphetamine-like agent used as an anorectic drug. Abuse of DEP has been reported and some restrictions of its use have been recently imposed. The conditioning place preference (CPP) paradigm was used to evaluate the reinforcing properties of DEP in adult male Wistar rats. After initial preferences were determined, animals weighing 250-300 g (N = 7 per group) were conditioned with DEP (10, 15 or 20 mg/kg). Only the dose of 15 mg/kg produced a significant place preference (358 ± 39 vs 565 ± 48 s). Pretreatment with the D1 antagonist SCH 23390 (0.05 mg/kg, sc) 10 min before DEP (15 mg/kg, ip) blocked DEP-induced CPP (418 ± 37 vs 389 ± 31 s) while haloperidol (0.5 mg/kg, ip), a D2 antagonist, 15 min before DEP was ineffective in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diethylpropion; Conditioning place preference; Reinforcing effect; Dopamine receptors.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400014

Assessment of the degree of contamination of rat germ cell preparations using specific cDNA probes
Provedor de dados: 56
Recent reports showing a decrease in sperm count in men have brought new concerns about male infertility. Animal models have been widely used to provide some relevant information about the human male gamete, and extrapolations are made to men and to the clinical context. The present study assesses one of the methods used for separation of germ cells of the adult rat testis, namely centrifugal elutriation followed by density gradients (Percoll®). This method was chosen since it presents the best results for cell purity in separating germ cells from the rat testis. A comparison between continuous and discontinuous Percoll® gradients was performed in order to identify the best type of gradient to separate the cells. Maximal cell purity was obtained for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rat testis; Germ cell separation; Percoll; Molecular analysis; Transition protein 2 (TP2); Mitochondrial cytochrome C oxidase II (COX II); Sulfated glycoprotein 1 (SGP1).
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300007

Structural properties of lipid reconstructs and lipid composition of normotensive and hypertensive rat vascular smooth muscle cell membranes
Provedor de dados: 56
Autores: Oliveira,T.R.; Lamy,M.T.; De Paula,U.M.; Guimarães,L.L.; Toledo,M.S.; Takahashi,H.K.; Straus,A.H.; Lindsey,C.J.; Paiva,T.B.
Multiple cell membrane alterations have been reported to be the cause of various forms of hypertension. The present study focuses on the lipid portion of the membranes, characterizing the microviscosity of membranes reconstituted with lipids extracted from the aorta and mesenteric arteries of spontaneously hypertensive (SHR) and normotensive control rat strains (WKY and NWR). Membrane-incorporated phospholipid spin labels were used to monitor the bilayer structure at different depths. The packing of lipids extracted from both aorta and mesenteric arteries of normotensive and hypertensive rats was similar. Lipid extract analysis showed similar phospholipid composition for all membranes. However, cholesterol content was lower in SHR arteries than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smooth muscle cell lipids; Membrane microviscosity; Spontaneously hypertensive rats; Lipid composition; Spin label; Electron spin resonance.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900012

In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
Provedor de dados: 56
Autores: Sun,Dao-Cai; Li,De-Hua; Ji,Hui-Cang; Rao,Guo-Zhou; Liang,Li-Hua; Ma,Ai-Jie; Xie,Chao; Zou,Gui-Ke; Song,Ying-Liang.
In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in vitro using the tissue explants adherent method. The biological properties of these cells were characterized using the following methods: alkaline phosphatase (ALP) chemical staining for cell viability, Alizarin red staining for osteogenic characteristics, MTT test for cell proliferation, enzyme dynamics for ALP contents, radio-immunoassay for bone gla protein (BGP) concentration, and ELISA for the concentration of type I collagen (COL-I) in the supernatant....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar bone; Cell culture; Implant; Osteoblast; Type 2 diabetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600005

Implications of ischemic penumbra for the diagnosis of brain death
Provedor de dados: 56
Autores: Coimbra,C.G.
The data reviewed here suggest the possibility that a global reduction of blood supply to the whole brain or solely to the infratentorial structures down to the range of ischemic penumbra for several hours or a few days may lead to misdiagnosis of irreversible brain or brain stem damage in a subset of deeply comatose patients with cephalic areflexia. The following proposals are advanced: 1) the lack of any set of clinically detectable brain functions does not provide a safe diagnosis of brain or brain stem death; 2) apnea testing may induce irreversible brain damage and should be abandoned; 3) moderate hypothermia, antipyresis, prevention of arterial hypotension, and occasionally intra-arterial thrombolysis may contribute to good recovery of a possibly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemic penumbra; Cerebral ischemia; Intracranial pressure; Traumatic brain injury; Brain death; Hypothermia; Thrombolysis; Apnea test.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200005

Delta sleep instability in children with chronic arthritis
Provedor de dados: 56
Autores: Lopes,M.C.; Guilleminault,C.; Rosa,A.; Passarelli,C.; Roizenblatt,S.; Tufik,S.
The objective of the present study was to evaluate the expression of a cyclic alternating pattern (CAP) in slow wave sleep (SWS) in children with the well-defined chronic syndrome juvenile idiopathic arthritis (JIA). Twelve patients (9-17 years of age), 7 girls, with JIA were compared to matched controls by age, pubertal stage and gender. After one night of habituation in the sleep laboratory, sleep measurements were obtained by standard polysomnography with conventional sleep scoring and additional CAP analyses. The sleep parameters of the JIA and control groups were similar for sleep efficiency (91.1 ± 6.7 vs 95.8 ± 4.0), sleep stage in minutes: stage 1 (16.8 ± 8.5 vs 17.8 ± 4.0), stage 2 (251.9 ± 41 vs 262.8 ± 38.1), stage 3 (17.0 ± 6.0 vs 15.1 ± 5.7),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Chronic-arthritis; Cyclic alternating pattern; Sleep fragmentation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000018

Effect of metabolic syndrome and of its individual components on renal function of patients with type 2 diabetes mellitus
Provedor de dados: 56
Autores: Moehlecke,M.; Leitão,C.B.; Kramer,C.K.; Rodrigues,T.C.; Nickel,C.; Silveiro,S.P.; Gross,J.L.; Canani,L.H.
The objective of this study was to evaluate the effect of metabolic syndrome (MetS) and its individual components on the renal function of patients with type 2 diabetes mellitus (DM). A cross-sectional study was performed in 842 type 2 DM patients. A clinical and laboratory evaluation, including estimated glomerular filtration rate (eGFR) calculated by the modification of diet in renal disease formula, was performed. MetS was defined according to National Cholesterol Education Program - Adult Treatment Panel III criteria. Mean patient age was 57.9 ± 10.1 years and 313 (37.2%) patients were males. MetS was detected in 662 (78.6%) patients. A progressive reduction in eGFR was observed as the number of individual MetS components increased (one: 98.2 ± 30.8;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Type 2 diabetes mellitus; Glomerular filtration rate; Modification of diet in renal disease.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700012

Functional differences between two morphologically distinct cell subpopulations within a human colorectal carcinoma cell line
Provedor de dados: 56
Autores: Solimene,A.C.C.; Carneiro,C.R.W.; Melati,I.; Lopes,J.D.
The LISP-I human colorectal adenocarcinoma cell line was isolated from a hepatic metastasis at the Ludwig Institute, São Paulo, SP, Brazil. The objective of the present study was to isolate morphologically different subpopulations within the LISP-I cell line, and characterize some of their behavioral aspects such as adhesion to and migration towards extracellular matrix components, expression of intercellular adhesion molecules and tumorigenicity in vitro. Once isolated, the subpopulations were submitted to adhesion and migration assays on laminin and fibronectin (crucial proteins to invasion and metastasis), as well as to anchorage-independent growth. Two morphologically different subpopulations were isolated: LISP-A10 and LISP-E11. LISP-A10 presents a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcinoembryonic antigen; CEA; Colorectal carcinoma cell line; Laminin; Fibronectin; Desmoglein; Metastasis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500014

Infrequent p53 gene alterations in ulcerative colitis
Provedor de dados: 56
Autores: Mattar,R.; Alexandrino,A.M.; Laudanna,A.A.
The purpose of this study was to determine whether point mutations and loss of the p53 gene take place in ulcerative colitis which is histologically negative for dysplasia. DNA was extracted from 13 frozen rectal or colon biopsies and blood samples. Ulcerative colitis was classified histologically as active (10 cases) and inactive (3 cases). Exons 5-8 were amplified by PCR, treated with exonuclease and shrimp alkaline phosphatase and sequenced by the dideoxy chain termination method with the Sequenase Version 2.0 DNA sequencing kit. PCR products of intron 6 and exon 4 were digested with MspI and AccII, respectively, for RFLP analysis. No p53 gene mutation was detected in these cases. The number of informative patients for loss of heterozygosity (LOH) at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P53 gene; Ulcerative colitis; LOH; Mutation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900005

Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
Provedor de dados: 56
Autores: Santos,M.F.M.; Oliveira,F.C.A.C.; Kishimoto,R.K.; Borri,D.; Santos,F.P.S.; Campregher,P.V.; Silveira,P.A.A.; Hamerschlak,N.; Mangueira,C.L.P.; Duarte,F.B.; Crepaldi,A.H.; Salvino,M.A.; Velloso,E.D.R.P.
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Karyotype; Leukemia; Myelodysplasia; Myeloproliferative disease.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608

Association of leprosy with HLA-DR2 in a Southern Brazilian population
Provedor de dados: 56
The association between HLA specificities and leprosy was investigated in a Southern Brazilian population. One hundred and twenty-one patients and 147 controls were typed for HLA-A, B, Cw, DR and DQ. Patients were subdivided into the following subgroups, according to clinical, histological and immunological criteria: lepromatous (N = 55), tuberculoid (N = 32), dimorphous (N = 20), and indeterminate (N = 14). The frequencies of HLA specificities were compared between the total group of patients and controls, and between the same controls and each subgroup of patients. After correction of the probabilities, deviations were not significant, except for the DR2 specificity, which presented a frequency of 44.2% in the total group of patients and 56.3% in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HLA; Leprosy; Mycobacterium leprae.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100008

Sildenafil decreases rat tracheal hyperresponsiveness to carbachol and changes canonical transient receptor potential gene expression after antigen challenge
Provedor de dados: 56
Autores: Sousa,C.T.; Brito,T.S.; Lima,F.J.B.; Siqueira,R.J.B.; Magalhães,P.J.C.; Lima,A.A.M.; Santos,A.A.; Havt,A.
Inhibition of type-5 phosphodiesterase by sildenafil decreases capacitative Ca2+ entry mediated by transient receptor potential proteins (TRPs) in the pulmonary artery. These families of channels, especially the canonical TRP (TRPC) subfamily, may be involved in the development of bronchial hyperresponsiveness, a hallmark of asthma. In the present study, we evaluated i) the effects of sildenafil on tracheal rings of rats subjected to antigen challenge, ii) whether the extent of TRPC gene expression may be modified by antigen challenge, and iii) whether inhibition of type-5 phosphodiesterase (PDE5) may alter TRPC gene expression after antigen challenge. Sildenafil (0.1 µM to 0.6 mM) fully relaxed carbachol-induced contractions in isolated tracheal rings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Ovalbumin; Capacitative Ca2+ entry; Transient receptor potential proteins.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600010

Negative inotropic and chronotropic effects on the guinea pig atrium of extracts obtained from Averrhoa carambola L. leaves
Provedor de dados: 56
Autores: Vasconcelos,C.M.L.; Araújo,M.S.; Silva,B.A.; Conde-Garcia,E.A.
It has been reported that star fruit can lead to a fatal outcome in uremic patients. The intoxication syndrome consists of hiccups, mental confusion, dizziness, and vomiting. On the other hand, folk medicine uses teas and infusions of carambola leaves to treat headache, vomiting, cough, insomnia, and diabetes. This motivated us to determine if Averrhoa carambola can act on the contractility and automaticity of the guinea pig heart. We measured the atrial isometric force in stimulated left atria and determined the chronotropic changes in spontaneously beating right atria. The carambola leaf extracts (1.5 mg/ml) abolished the contractile force in a concentration-dependent manner. Among the crude, methanolic, ethanolic, aqueous, and acetic extracts, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Averrhoa carambola L.; Star fruit; Inotropism; Chronotropism; Guinea pig atrium; Ca2+ channel blockade.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700015

Effect of microRNA-21 on the proliferation of human degenerated nucleus pulposus by targeting programmed cell death 4
Provedor de dados: 56
Autores: Chen,B.; Huang,S.G.; Ju,L.; Li,M.; Nie,F.F.; Zhang,Y.; Zhang,Y.H.; Chen,X.; Gao,F.
This study aims to explore the effect of microRNA-21 (miR-21) on the proliferation of human degenerated nucleus pulposus (NP) by targeting programmed cell death 4 (PDCD4) tumor suppressor. NP tissues were collected from 20 intervertebral disc degeneration (IDD) patients, and from 5 patients with traumatic spine fracture. MiR-21 expressions were tested. NP cells from IDD patients were collected and divided into blank control group, negative control group (transfected with miR-21 negative sequences), miR-21 inhibitor group (transfected with miR-21 inhibitors), miR-21 mimics group (transfected with miR-21 mimics) and PDCD4 siRNA group (transfected with PDCD4 siRNAs). Cell growth was estimated by Cell Counting Kit-8; PDCD4, MMP-2,MMP-9 mRNA expressions were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intervertebral disc degeneration; MicroRNA-21; Human nucleus pulposus cells; Matrix metalloproteinase-2; Matrix metalloproteinase-9; Target gene.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600602

Expression and purification of the non-tagged LipL32 of pathogenic Leptospira
Provedor de dados: 56
Autores: Hauk,P.; Carvalho,E.; Ho,P.L.
Leptospirosis is a reemerging infectious disease and the most disseminated zoonosis worldwide. A leptospiral surface protein, LipL32, only occurs in pathogenic Leptospira, and is the most abundant protein on the bacterial surface, being described as an important factor in host immunogenic response and also in bacterial infection. We describe here an alternative and simple purification protocol for non-tagged recombinant LipL32. The recombinant LipL32(21-272) was expressed in Escherichia coli without His-tag or any other tag used to facilitate recombinant protein purification. The recombinant protein was expressed in the soluble form, and the purification was based on ion exchange (anionic and cationic) and hydrophobic interactions. The final purification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigen; LipL32; Pathogenic Leptospira; Non-tagged protein purification; Diagnosis; Vaccine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400005

miR-27a protects human mitral valve interstitial cell from TNF-α-induced inflammatory injury via up-regulation of NELL-1
Provedor de dados: 56
Autores: Chen,Honglei; Zhang,Zhixu; Zhang,Li; Wang,Junzhi; Zhang,Minghui; Zhu,Bin.
MicroRNAs (miRNAs) have been reported to be associated with heart valve disease, which can be caused by inflammation. This study aimed to investigate the functional impacts of miR-27a on TNF-α-induced inflammatory injury in human mitral valve interstitial cells (hMVICs). hMVICs were subjected to 40 ng/mL TNF-α for 48 h, before which the expressions of miR-27a and NELL-1 in hMVICs were altered by stable transfection. Trypan blue staining, BrdU incorporation assay, flow cytometry detection, ELISA, and western blot assay were performed to detect cell proliferation, apoptosis, and the release of proinflammatory cytokines. We found that miR-27a was lowly expressed in response to TNF-α exposure in hMVICs. Overexpression of miR-27a rescued hMVICs from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-27a; Heart valve disease (HVD); Human mitral valve interstitial cell (hMVIC); TNF-α; Inflammatory injury; NELL-1.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600603

Manipulating Greek musical modes and tempo affects perceived musical emotion in musicians and nonmusicians
Provedor de dados: 56
Autores: Ramos,D.; Bueno,J.L.O.; Bigand,E.
The combined influence of tempo and mode on emotional responses to music was studied by crossing 7 changes in mode with 3 changes in tempo. Twenty-four musicians aged 19 to 25 years (12 males and 12 females) and 24 nonmusicians aged 17 to 25 years (12 males and 12 females) were required to perform two tasks: 1) listening to different musical excerpts, and 2) associating an emotion to them such as happiness, serenity, fear, anger, or sadness. ANOVA showed that increasing the tempo strongly affected the arousal (F(2,116) = 268.62, mean square error (MSE) = 0.6676, P &lt; 0.001) and, to a lesser extent, the valence of emotional responses (F(6,348) = 8.71, MSE = 0.6196, P &lt; 0.001). Changes in modes modulated the affective valence of the perceived emotions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Greek musical modes; Musical tempo; Emotional responses to music; Musical expertise.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200010

Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
Provedor de dados: 56
Autores: Merjan,A.J.; Kanashiro,C.A.; Krieger,J.E.; Han,S.W.; Paiva,A.C.M.
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Agonist-induced receptor internalization; Endocytosis; Fluorescence-tagged receptor; Nuclear angiotensin receptors; Renin-angiotensin system.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011

Nitric oxide modulation of the hypothalamo-neurohypophyseal system
Provedor de dados: 56
Autores: Kadekaro,M.
Nitric oxide (NO), a free radical gas produced endogenously from the amino acid L-arginine by NO synthase (NOS), has important functions in modulating vasopressin and oxytocin secretion from the hypothalamo-neurohypophyseal system. NO production is stimulated during increased functional activity of magnocellular neurons, in parallel with plastic changes of the supraoptic nucleus (SON) and paraventricular nucleus. Electrophysiological data recorded from the SON of hypothalamic slices indicate that NO inhibits firing of phasic and non-phasic neurons, while L-NAME, an NOS inhibitor, increases their activity. Results from measurement of neurohypophyseal hormones are more variable. Overall, however, it appears that NO, tonically produced in the forebrain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-NAME; Vasopressin; Oxytocin; CGMP; ODQ; Microdialysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400001

Effect of repeated restraint stress on memory in different tasks
Provedor de dados: 56
Autores: Gamaro,G.D.; Michalowski,M.B.; Catelli,D.H.; Xavier,M.H.; Dalmaz,C.
The present study investigated the effect of repeated stress applied to female rats on memory evaluated by three behavioral tasks: two-way shuttle avoidance, inhibitory avoidance and habituation to an open field. Repeated stress had different effects on rat behavior when different tasks were considered. In the two-way active avoidance test the stressed animals presented memory of the task, but their memory scores were impaired when compared to all other groups. In the habituation to the open field, only the control group showed a significant difference in the number of rearings between training and testing sessions, which is interpreted as an adequate memory of the task. In the handled and chronically stressed animals, on the other hand, no memory was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic stress; Memory; Inhibitory avoidance; Two-way active avoidance; Open field.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300015

Effects of terpineol on the compound action potential of the rat sciatic nerve
Provedor de dados: 56
Autores: Moreira,M.R.; Cruz,G.M.P.; Lopes,M.S.; Albuquerque,A.A.C.; Leal-Cardoso,J.H.
Terpineol, a volatile terpenoid alcohol of low toxicity, is widely used in the perfumery industry. It is an important chemical constituent of the essential oil of many plants with widespread applications in folk medicine and in aromatherapy. The effects of terpineol on the compound action potential (CAP) of rat sciatic nerve were studied. Terpineol induced a dose-dependent blockade of the CAP. At 100 µM, terpineol had no demonstrable effect. At 300 µM terpineol, peak-to-peak amplitude and conduction velocity of CAP were significantly reduced at the end of 180-min exposure of the nerve to the drug, from 3.28 ± 0.22 mV and 33.5 ± 7.05 m/s, respectively, to 1.91 ± 0.51 mV and 26.2 ± 4.55 m/s. At 600 µM, terpineol significantly reduced peak-to-peak amplitude...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Essential oil; Alpinia speciosa; Zingiberaceae; Sciatic nerve; Compound action potential; Nerve conduction velocity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000015

Opiatergic participation in the thirst-inhibiting effect of acute third ventricle injections of cadmium (Cd2+) and lead (Pb2+)
Provedor de dados: 56
Autores: De-Castro-e-Silva,E.; Luz,C.P.; Sarmento,C.; Nascimento,T.; Gonzalez,V.; Marinho,C.A.; Castro,L.; Oliveira,P.; Santana Jr.,P.; De-Oliveira,I.R.; De-Paula,S.; Lima,A.K.S.; Fregoneze,J.B.
We have previously demonstrated that acute third ventricle injections of both lead and cadmium prevent the dipsogenic response elicited by dehydration or by central injections of dipsogenic agents such as angiotensin II, carbachol and isoproterenol in rats. We have also shown that the antidipsogenic action of cadmium may be due, at least in part, to activation of thirst-inhibitory central serotonergic pathways. In the present paper we show that in Wistar male rats the antidipsogenic effect of both lead acetate (3.0 nmol/rat) and cadmium chloride (3.0 nmol/rat) may be partially dependent on the activation of brain opiatergic pathways since central injections of naloxone (82.5 nmol/rat), a non-selective opioid antagonist, blunt the thirst-inhibiting effect...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cadmium; Lead; Water intake; Opioids; Rats.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600013

Gender differences in serum CK-MB mass levels in healthy Braziliansubjects
Provedor de dados: 56
Autores: Araki,L.M.; Nogueira,A.A.R.; Mansur,A.P.
The creatine kinase-isoenzyme MB (CK-MB) mass assay is one of the laboratory tests used for the diagnosis of myocardial infarction. It is recommended, however, that reference limits should take gender and race into account. In the present study, we analyzed the plasma CK-MB mass and troponin levels of 244 healthy volunteers without a personal history of coronary artery disease and with no chronic diseases, muscular trauma or hypothyroidism, and not taking statins. The tests were performed with commercial kits, CK-MB mass turbo kit and Troponin I turbo kit, using the Immulite 1000 analyzer from Siemens Healthcare Diagnostic. The values were separated according to gender and showed significant differences by the Mann-Whitney test. Mean (± SD) CK-MB mass...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CK-MB mass; Gender; Healthy volunteers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300010

Pituitary glycoprotein hormone a-subunit secretion by cirrhotic patients
Provedor de dados: 56
Autores: Oliveira,M.C.; Pizarro,C.B.; Cassal,A.; Cremonese,R.; Vieira,J.G.H.
Secretion of the &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;-subunit of pituitary glycoprotein hormones usually follows the secretion of intact gonadotropins and is increased in gonadal failure and decreased in isolated gonadotropin deficiency. The aim of the present study was to determine the levels of the &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;-subunit in the serum of patients with cirrhosis of the liver and to compare the results obtained for eugonadal cirrhotic patients with those obtained for cirrhotic patients with hypogonadotropic hypogonadism. Forty-seven of 63 patients with cirrhosis (74.6%) presented hypogonadism (which was central in 45 cases and primary in 2), 7 were eugonadal, and 9 women were in normal menopause. The serum &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: &lt; FONT FACE=Symbol&gt; A&lt; /font&gt; -subunit pituitary gonadotropins hypogonadism cirrhosis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100011

Increase in skeletal muscle protein content by the ß-2 selective adrenergic agonist clenbuterol exacerbates hypoalbuminemia in rats fed a low-protein diet
Provedor de dados: 56
Autores: Sawaya,A.L.; Lunn,P.G.
This investigation examined how the nutritional status of rats fed a low-protein diet was affected when the animals were treated with the ß-2 selective agonist clenbuterol (CL). Males (4 weeks old) from an inbred, specific-pathogen-free strain of hooded rats maintained at the Dunn Nutritional Laboratory were used in the experiments (N = 6 rats per group). CL treatment (Ventipulmin, Boehringer-Ingelheim Ltd., 3.2 mg/kg diet for 2 weeks) caused an exacerbation of the symptoms associated with protein deficiency in rats. Plasma albumin concentrations, already low in rats fed a low-protein diet (group A), were further reduced in CL rats (A = 25.05 ± 0.31 vs CL = 23.64 ± 0.30 g/l, P&amp;lt;0.05). Total liver protein decreased below the level seen in either...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein-energy malnutrition; Rats; Clenbuterol; Hypoalbuminemia; Brown adipose tissue; SS-2 agonist.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600015

HFE gene mutations in Brazilian thalassemic patients
Provedor de dados: 56
Autores: Oliveira,T.M.; Souza,F.P.; Jardim,A.C.G.; Cordeiro,J.A.; Pinho,J.R.R.; Sitnik,R.; Estevão,I.F.; Bonini-Domingos,C.R.; Rahal,P.
Hereditary hemochromatosis is a disorder of iron metabolism characterized by increased iron intake and progressive storage and is related to mutations in the HFE gene. Interactions between thalassemia and hemochromatosis may further increase iron overload. The ethnic background of the Brazilian population is heterogeneous and studies analyzing the simultaneous presence of HFE and thalassemia-related mutations have not been carried out. The aim of this study was to evaluate the prevalence of the H63D, S65C and C282Y mutations in the HFE gene among 102 individuals with alpha-thalassemia and 168 beta-thalassemia heterozygotes and to compare them with 173 control individuals without hemoglobinopathies. The allelic frequencies found in these three groups were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HFE; H63D; S65C; C282Y; Thalassemia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200008

Histologic distribution of insulin and glucagon receptors
Provedor de dados: 56
Autores: Watanabe,M.; Hayasaki,H.; Tamayama,T.; Shimada,M.
Insulin and glucagon are the hormonal polypeptides secreted by the B and A cells of the endocrine pancreas, respectively. Their major physiologic effects are regulation of carbohydrate metabolism, but they have opposite effects. Insulin and glucagon have various physiologic roles, in addition to the regulation of carbohydrate metabolism. The physiologic effects of insulin and glucagon on the cell are initiated by the binding of each hormone to receptors on the target cells. Morphologic studies may be useful for relating biochemical, physiologic, and pharmacologic information on the receptors to an anatomic background. Receptor radioautography techniques using radioligands to label specific insulin and glucagon receptors have been successfully applied to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioautography; Insulin; Glucagon; Receptor; Distribution.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200008

Estrogen and progesterone promote breast cancer cell proliferation by inducing cyclin G1 expression
Provedor de dados: 56
Autores: Tian,J-M.; Ran,B.; Zhang,C-L.; Yan,D-M.; LI,X-H.
Breast cancer is the most common cause of cancer among women in most countries (WHO). Ovarian hormone disorder is thought to be associated with breast tumorigenesis. The present study investigated the effects of estrogen and progesterone administration on cell proliferation and underlying mechanisms in breast cancer MCF-7 cells. It was found that a single administration of estradiol (E2) or progesterone increased MCF-7 cell viability in a dose-dependent manner and promoted cell cycle progression by increasing the percentage of cells in the G2/M phase. A combination of E2 and progesterone led to a stronger effect than single treatment. Moreover, cyclin G1 was up-regulated by E2 and/or progesterone in MCF-7 cells. After knockdown of cyclin G1 in MCF-7 cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Estrogen; Progesterone; Cyclin G1; Cell proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300611

Antiproliferative effect of urolithin A, the ellagic acid-derived colonic metabolite, on hepatocellular carcinoma HepG2.2.15 cells by targeting Lin28a/let-7a axis
Provedor de dados: 56
Autores: Qiu,Zhenpeng; Zhou,Junxuan; Zhang,Cong; Cheng,Ye; Hu,Junjie; Zheng,Guohua.
An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively. Results revealed that urolithin A induced cytotoxicity in HepG2.2.15 cells, which was accompanied by the cleavage of caspase-3 protein and down-regulation of Bcl-2/Bax ratio. Moreover, urolithin A suppressed the protein expressions of Sp-1, Lin28a,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urolithin A; Hepatocellular carcinoma; Lin28a; Let-7a; Cell proliferation; HBx.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700602

Differential expression of integrin subunits on adherent and nonadherent mast cells
Provedor de dados: 56
Autores: Grodzki,A.C.G.; Dávila Pástor,M.V.; Sousa,J.F.; Oliver,C.; Jamur,M.C.
Mast cell progenitors arise in bone marrow and then migrate to peripheral tissues where they mature. It is presumed that integrin receptors are involved in their migration and homing. In the present study, the expression of various integrin subunits was investigated in three systems of adherent and nonadherent mast cells. Mesentery mast cells, freshly isolated bone marrow-derived mast cells (BMMC) and RBL-2H3 cells grown attached to tissue culture flasks are all adherent mast cells and peritoneal mast cells, and cultured BMMC and RBL-2H3 cells grown in suspension represent nonadherent mast cell populations. Pure populations of mast cells were immunomagnetically isolated from bone marrow, mesentery and peritoneal lavage using the mast cell-specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mast cells; Integrin; Mouse.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800017

Fine-tuning of defensive behaviors in the dorsal periaqueductal gray by atypical neurotransmitters
Provedor de dados: 56
Autores: Fogaça,M.V.; Lisboa,S.F.; Aguiar,D.C.; Moreira,F.A.; Gomes,F.V.; Casarotto,P.C.; Guimarães,F.S.
This paper presents an up-to-date review of the evidence indicating that atypical neurotransmitters such as nitric oxide (NO) and endocannabinoids (eCBs) play an important role in the regulation of aversive responses in the periaqueductal gray (PAG). Among the results supporting this role, several studies have shown that inhibitors of neuronal NO synthase or cannabinoid receptor type 1 (CB1) receptor agonists cause clear anxiolytic responses when injected into this region. The nitrergic and eCB systems can regulate the activity of classical neurotransmitters such as glutamate and γ-aminobutyric acid (GABA) that control PAG activity. We propose that they exert a ‘fine-tuning’ regulatory control of defensive responses in this area. This control, however, is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocannabinoid; Anandamide; Nitric oxide; Endovanilloid; TRPV1.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400010

Endothelium-dependent and -independent hepatic artery vasodilatation is not impaired in a canine model of liver ischemia-reperfusion injury
Provedor de dados: 56
Autores: Miranda,L.C.; Viaro,F.; Ceneviva,R.; Evora,P.R.B.
We investigated whether hepatic artery endothelium may be the earliest site of injury consequent to liver ischemia and reperfusion. Twenty-four heartworm-free mongrel dogs of either sex exposed to liver ischemia/reperfusion in vivo were randomized into four experimental groups (N = 6): a) control, sham-operated dogs, b) dogs subjected to 60 min of ischemia, c) dogs subjected to 30 min of ischemia and 60 min of reperfusion, and d) animals subjected to 45 min of ischemia and 120 min of reperfusion. The nitric oxide endothelium-dependent relaxation of hepatic artery rings contracted with prostaglandin F2a and exposed to increasing concentrations of acetylcholine, calcium ionophore A23187, sodium fluoride, phospholipase-C, poly-L-arginine, isoproterenol, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelium; Hepatic artery; Nitric oxide; Liver; Ischemia/reperfusion injury.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600016

Effects of ethanol intake on retinol concentration in the milk of lactating rats
Provedor de dados: 56
Autores: Albuquerque,K.T.; Ramalho,R.A.; Soares,A.G.; Tavares-do-Carmo,M.G.
The effect of the consumption of ethanol (5%) on retinol concentration in milk was studied in the rat on day 12 after delivery, together with the evolution of dam body weight and pup growth rate. Female Wistar rats receiving alcohol (5%) in drinking water during lactation (N = 7) were compared to normal controls fed ad libitum (N = 6). The mean maternal alcohol intake was 3.96 ± 0.23 g/kg body weight per day. To determine retinol levels in milk we used the Bessey and Lowry method, modified by Araújo and Flores ((1978) Clinical Chemistry, 24: 386-392). The pups were separated from dams for a 2-4-h period, after which the dams were injected intraperitoneally with anesthetic and oxytocin. The concentration of retinol in milk was 162.88 ± 10.60 µg/dl in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lactation; Ethanol; Retinol; Milk; Rat.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700007

Anxiety and depression symptoms in recurrent painful renal lithiasis colic
Provedor de dados: 56
Autores: Diniz,D.H.M.P.; Blay,S.L.; Schor,N.
Several studies have reported that symptoms of anxiety and depression are significantly associated with diseases characterized by painful crises. However, there is little information about the psychological aspects of recurrent painful episodes of renal stone disease. Our objective was to evaluate the association of symptoms of anxiety, depression and recurrent painful renal colic in a case-control study involving 64 subjects (32 cases/32 controls) matched for age and sex. Cases were outpatients with a confirmed diagnosis of nephrolithiasis as per their case history, physical examination, image examination and other laboratory exams. Patients had a history of at least two episodes within a 3-year period, and were currently in an intercrisis interval. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal colic; Lithiasis; Symptoms of anxiety; Symptoms of depression.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700009

Dexamethasone blocks the migration of the human neuroblastoma cell line SK-N-SH
Provedor de dados: 56
Autores: Casulari,L.A.; Dondi,D.; Maggi,R.; Demissie,M.; Piccolella,M.; Piva,F.
Glucocorticoids (Gc) influence the differentiation of neural crest-derived cells such as those composing sympathoadrenal tumors like pheochromocytomas, as well as neuroblastomas and gangliomas. In order to obtain further information on the effects of Gc on cells evolving from the neural crest, we have used the human neuroblastoma cell line SK-N-SH to analyze: 1) the presence and the binding characteristics of Gc receptors in these cells, 2) the effect of dexamethasone (Dex) on the migration of SK-N-SH cells, and 3) the effect of Dex on the organization of the cytoskeleton of SK-N-SH cells. We show that: 1) receptors that bind [³H]-Dex with high affinity and high capacity (Kd of 9.6 nM, Bmax of 47 fmol/mg cytosolic protein, corresponding to 28,303...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human neuroblastoma; SK-N-SH cells; Glucocorticoids; Migration; Cytoskeleton.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900011

Fecal estradiol and progesterone metabolite levels in the three-toed sloth (Bradypus variegatus)
Provedor de dados: 56
Autores: Mühlbauer,M.; Duarte,D.P.F.; Gilmore,D.P.; da Costa,C.P.
The present study was carried out to assess the possibility of measuring fecal steroid hormone metabolites as a noninvasive technique for monitoring reproductive function in the three-toed sloth, Bradypus variegatus. Levels of the estradiol (E2) and progesterone (P4) metabolites were measured by radioimmunoassay in fecal samples collected over 12 weeks from 4 captive female B. variegatus sloths. The validation of the radioimmunoassay for evaluation of fecal steroid metabolites was carried out by collecting 10 blood samples on the same day as defecation. There was a significant direct correlation between the plasma and fecal E2 and P4 levels (P &lt; 0.05, Pearson's test), thereby validating this noninvasive technique for the study of the estrous cycle in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sloth; Fecal estrogen and progestogen metabolites; Noninvasive monitoring; Estrous cycle; Fecal hormones.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200017

c-erbB-2 expression and nuclear pleomorphism in canine mammary tumors
Provedor de dados: 56
Autores: Dutra,A.P.; Granja,N.V.M.; Schmitt,F.C.; Cassali,G.D.
The objective of the present investigation was to study the expression of c-erbB-2 and MIB-1 and try to associate them with morphological features of the cell such as nuclear pleomorphism, mitotic count and histological grade in a series of 70 canine mammary gland tumors, 22 of them benign and 48 malignant. Tumors were collected at the Veterinary Hospital of UFMG (Brazil) and the Veterinary Faculty of Porto University (Portugal). c-erbB-2 expression was determined according to the guidelines provided by the manufacturer of the HercepTest system and nuclear pleomorphism, mitotic count and histological grade according the Elston and Ellis grading system. The HercepTest is the FDA-approved in vitro diagnostic test marketed by Dako. It is a semi-quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine mammary tumors; Nuclear pleomorphism; Breast cancer; C-erbB-2 expression; MIB-1 expression.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100013

The influence of septal lesions on sodium and water retention induced by Walker 256 tumor
Provedor de dados: 56
Autores: Guimarães,F.; Rettori,O.; Vieira-Matos,A.N.; Fernandes,G.A.
In the course of studies on the effects of septal area lesions on neuroimmunomodulation and Walker 256 tumor development, it was observed that tumor-induced sodium and water retention was less marked in lesioned than in non-lesioned rats. In the present study possible mechanisms involved in this phenomenon were investigated. The experiments were performed in septal-lesioned (LW; N = 15) and sham-operated (SW; N = 7) 8-week-old male Wistar rats, which received multifocal simultaneous subcutaneous (sc) inoculations of Walker 256 tumor cells about 30 days after the stereotaxic surgery. Control groups (no tumor, sham-operated food-restricted (SFR), N = 7) and lesioned food-restricted (LFR, N = 10) were subjected to a feeding pattern similar to that observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Na+ retention; Secondary diuretic response; Tumor systemic effects; Septal area lesion; Multifocal tumor inoculation; Walker 256 tumor.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300010

Association between MFN2 gene polymorphisms and the risk and prognosis of acute liver failure: a case-control study in a Chinese population
Provedor de dados: 56
Autores: Wei,Y.-L.; Tian,Q.; Zhao,X.-X.; Qiu,G.-Z.; Xu,Y.
This study aimed to determine the role of mitofusin 2 (MFN2) gene polymorphisms in the risk and prognosis of acute liver failure (ALF). A total of 298 blood samples were collected from 138 ALF patients (case group) and 160 healthy participants (control group). Coagulation function, glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), total bilirubin (TB), blood ammonia and lactic acid (LA) were measured. The predictive evaluation of MFN2 gene polymorphisms in the risk and prognosis of ALF patients was estimated using Kaplan-Meier survival analysis, haplotype analysis, binary logistic regression analysis and Cox regression analysis. Higher levels of GPT, GOT, TB, blood ammonia and LA were observed in ALF patients with the GG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute liver failure; MFN2; Gene polymorphism; Risk; Prognosis; Chinese population.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600605

Proliferative signaling initiated in ACTH receptors
Provedor de dados: 56
Autores: Lotfi,C.F.P.; Lepique,A.P.; Forti,F.L.; Schwindt,T.T.; Eichler,C.B.; Santos,M.O.; Rebustini,I.T.; Hajj,G.N.M.; Juliano,L.; Armelin,H.A.
This article reviews recent results of studies aiming to elucidate modes of integrating signals initiated in ACTH receptors and FGF2 receptors, within the network system of signal transduction found in Y1 adrenocortical cells. These modes of signal integration should be central to the mechanisms underlying the regulation of the G0-&gt;G1-&gt;S transition in the adrenal cell cycle. FGF2 elicits a strong mitogenic response in G0/G1-arrested Y1 adrenocortical cells, that includes a) rapid and transient activation of extracellular signal-regulated kinases-mitogen-activated protein kinases (ERK-MAPK) (2 to 10 min), b) transcription activation of c-fos, c-jun and c-myc genes (10 to 30 min), c) induction of c-Fos and c-Myc proteins by 1 h and cyclin D1 protein by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACTH; FGF2; Signal transduction; MAP kinases; Early response genes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000002

Anticonvulsant and antiarrhythmic effects of nifedipine in rats prone to audiogenic seizures
Provedor de dados: 56
Autores: Damasceno,D.D.; Ferreira,A.J.; Doretto,M.C.; Almeida,A.P.
Calcium ion participates in the regulation of neural transmission and the presynaptic release of neurotransmitters. It is also involved in epileptic events, cardiac arrhythmias and abnormal conduction of stimuli. The purpose of the present study was to evaluate the effects of nifedipine, a calcium channel blocker, on epileptic seizures and on reperfusion arrhythmias in rats prone to audiogenic epileptic seizures (Wistar audiogenic rats, WAR) and in normal Wistar rats (N = 6/group). The seizure severity index was applied after an intraperitoneal injection of 20 or 40 mg/kg nifedipine (N20 and N40 groups, respectively). The Langendorff technique was used to analyze cardiac function, as well as the incidence and severity of the reperfusion arrhythmias after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nifedipine; Calcium channel antagonism; Audiogenic seizures; Epilepsy; Arrhythmias.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100010

Testosterone therapy delays cardiomyocyte aging via an androgen receptor-independent pathway
Provedor de dados: 56
Autores: Zhang,L.; Wu,S.Z.; Ruan,Y.J.; Hong,L.; Xing,X.W.; Lai,W.Y.
The testicular feminized (Tfm) mouse carries a nonfunctional androgen receptor (AR) and reduced circulating testosterone levels. We used Tfm and castrated mice to determine whether testosterone modulates markers of aging in cardiomyocytes via its classic AR-dependent pathway or conversion to estradiol. Male littermates and Tfm mice were divided into 6 experimental groups. Castrated littermates (group 1) and sham-operated Tfm mice (group 2, N = 8 each) received testosterone. Sham-operated Tfm mice received testosterone in combination with the aromatase inhibitor anastrazole (group 3, N = 7). Castrated littermates (group 4) and sham-operated untreated Tfm mice (group 5) were used as controls (N = 8 and 7, respectively). An additional control group (group 6)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Testosterone; Cardiomyocyte; Androgen receptor; Estradiol; Tfm mice.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100007

Andrographolide protects mouse astrocytes against hypoxia injury by promoting autophagy and S100B expression
Provedor de dados: 56
Autores: Du,Juan; Zhang,Chunyan; Na,Xueqing; Li,Aizhi; Zhang,Qingfeng; Li,Kezhong; Ding,Yongbo.
Andrographolide (ANDRO) has been studied for its immunomodulation, anti-inflammatory, and neuroprotection effects. Because brain hypoxia is the most common factor of secondary brain injury after traumatic brain injury, we studied the role and possible mechanism of ANDRO in this process using hypoxia-injured astrocytes. Mouse cortical astrocytes C8-D1A (astrocyte type I clone from C57/BL6 strains) were subjected to 3 and 21% of O2 for various times (0–12 h) to establish an astrocyte hypoxia injury model in vitro. After hypoxia and ANDRO administration, the changes in cell viability and apoptosis were assessed using CCK-8 and flow cytometry. Expression changes in apoptosis-related proteins, autophagy-related proteins, main factors of JNK pathway, ATG5, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Andrographolide; Hypoxia-injured astrocytes; JNK pathway; S100B; ATG5.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600604

Systemic injection of an H4 receptor agonist induces a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex in mice
Provedor de dados: 56
Autores: Fernandes,C.E.M.; Serafim,K.R.; Gianlorenço,A.C.L.; Mattioli,R.
Studies have shown that an injection with the histamine H4 receptor agonist VUF-8430 modulates emotional memory processes. In the present study, the aim was to verify if intraperitoneal (ip) injection of VUF-8430 (500 ng/kg) in mice affects the synthesis of proteins required for memory consolidation processes by activating the phosphorylation of CREB (pCREB) in classical structures linked to emotional memory (prefrontal cortex, amygdala, and hippocampus) and the cerebellar vermis, a structure that has also been recently implicated in emotional memory. The results obtained using western blot analysis demonstrated that VUF-8430 induced a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex, suggesting that this dose impaired the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CREB; PCREB; VUF-8430; Western blotting; Emotional memory; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500603

Development of VEGF-loaded PLGA matrices in association with mesenchymal stem cells for tissue engineering
Provedor de dados: 56
Autores: Rosa,A.R.; Steffens,D.; Santi,B.; Quintiliano,K.; Steffen,N.; Pilger,D.A.; Pranke,P.
The association of bioactive molecules, such as vascular endothelial growth factor (VEGF), with nanofibers facilitates their controlled release, which could contribute to cellular migration and differentiation in tissue regeneration. In this research, the influence of their incorporation on a polylactic-co-glycolic acid (PLGA) scaffold produced by electrospinning on cell adhesion and viability and cytotoxicity was carried out in three groups: 1) PLGA/BSA/VEGF; 2) PLGA/BSA, and 3) PLGA. Morphology, fiber diameter, contact angle, loading efficiency and controlled release of VEGF of the biomaterials, among others, were measured. The nanofibers showed smooth surfaces without beads and with interconnected pores. PLGA/BSA/VEGF showed the smallest water contact...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emulsion electrospinning; VEGF; Stem cells; PLGA; Biomaterials.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900601

Participation of the cholinergic system in the ethanol-induced suppression of paradoxical sleep in rats
Provedor de dados: 56
Autores: Papale,L.A.; Andersen,M.L.; Perry,J.C.; Tufik,S.
Sleep disturbance is among the many consequences of ethanol abuse in both humans and rodents. Ethanol consumption can reduce REM or paradoxical sleep (PS) in humans and rats, respectively. The first aim of this study was to develop an animal model of ethanol-induced PS suppression. This model administered intragastrically (by gavage) to male Wistar rats (3 months old, 200-250 g) 0.5 to 3.5 g/kg ethanol. The 3.5 g/kg dose of ethanol suppressed the PS stage compared with the vehicle group (distilled water) during the first 2-h interval (0-2 h; 1.3 vs 10.2; P &lt; 0.001). The second aim of this study was to investigate the mechanisms by which ethanol suppresses PS. We examined the effects of cholinergic drug pretreatment. The cholinergic system was chosen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholinergic; Atropine; Pilocarpine; Ethanol; Paradoxical sleep; Electroencephalography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900007

An interleukin-33/ST2 signaling deficiency reduces overt pain-like behaviors in mice
Provedor de dados: 56
Autores: Magro,D.A.C.; Hohmann,M.S.N.; Mizokami,S.S.; Cunha,T.M.; Alves-Filho,J.C.; Casagrande,R.; Ferreira,S.H.; Liew,F.Y.; Cunha,F.Q.; Verri Jr,W.A.
Interleukin (IL)-33, the most recent member of the IL family of cytokines, signals through the ST2 receptor. IL-33/ST2 signaling mediates antigen challenge-induced mechanical hyperalgesia in the joints and cutaneous tissues of immunized mice. The present study asked whether IL-33/ST2 signaling is relevant to overt pain-like behaviors in mice. Acetic acid and phenyl-p-benzoquinone induced significant writhing responses in wild-type (WT) mice; this overt nociceptive behavior was reduced in ST2-deficient mice. In an antigen-challenge model, ST2-deficient immunized mice had reduced induced flinch and licking overt pain-like behaviors. In the formalin test, ST2-deficient mice also presented reduced flinch and licking responses, compared with WT mice. Naive WT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-33; ST2; Pain; Nociception; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700601

Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion
Provedor de dados: 56
Autores: Zhang,J.P.; Li,N.; Bai,W.Z.; Qiu,X.C.; Ma,B.A.; Zhou,Y.; Fan,Q.Y.; Shan,L.Q.
Notch signaling plays a vital role in tumorigenicity and tumor progression by regulating proliferation, invasion, and the tumor microenvironment. Previous research by our group indicated that Notch ligand Delta-like 1 (Dll1) is involved in angiogenesis in melanoma, and we noticed that it took a longer time to trypsinize Dll1-expressing B16 melanoma cells than the control cells. In this article, we extended our study to investigate the effects of Dll1 on tumor cell adhesion and metastasis. Dll1 overexpression activated Notch signaling in B16 tumor cells and significantly enhanced the adhering capacity of B16 tumor cells both in vitro and in vivo. B16-Dll1 cells also had a higher metastatic potential than their counterpart in the mouse model of lung...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Delta-like 1; Notch signaling; Melanoma; Metastasis; Adhesion; N-cadherin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400299

Effect of topical dorzolamide on rabbit central corneal thickness
Provedor de dados: 56
Autores: Almeida Jr.,G.C.; Faria e Souza,S.J.
Our objective was to study the effect of dorzolamide on corneal hydration in an 18-week controlled experiment using ultrasonic pachymetry. Twenty-eight male rabbits were divided randomly into four groups. The 7 rabbits in each group received eye drops containing either 2% (w/v) dorzolamide or placebo in their right eye, or in their left eye. The 2% dorzolamide rabbits were treated every 8 h. Fellow eyes are defined as eyes which did not receive either dorzolamide or placebo. The study was blind for both the person who applied the drug and the one who performed the pachymetry. The effect of treatments is reported on the basis of the percentage of pachymetric variation compared to the measurement made before drug application. There was no significant...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dorzolamide; Corneal thickness; Carbonic anhydrase inhibition; Ultrasonic pachymetry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200015

Vascular supply of the central nervous system of the land snail Megalobulimus oblongus (Gastropoda, Pulmonata)
Provedor de dados: 56
Autores: Nóblega,H.G.; Missaglia,V.; Stenert,C.; Faccioni-Heuser,M.C.; Achaval,M.
The vascularization of the central nervous system of the snail Megalobulimus oblongus was studied by injection of carmine-gelatin solution into the arterial system and using a histochemical technique for the detection of alkaline phosphatase. The central nervous system of M. oblongus is irrigated by the anterior aorta, from which a series of small branches emerge that supply the subesophageal nervous ganglia. In turn, these branches give rise to a series of smaller vessels that irrigate the buccal bulb, the anterior portion of the foot, the cerebral ganglia, the dorsal body gland, and the anterior portion of the reproductive system. No hemolymph vessels were detected within nervous tissue although such vessels were found in the periganglionic connective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastropods; Central nervous system; Vascular supply; Trypan blue; Carmine-gelatin; Alkaline phosphatase activity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900016

Mechanisms involved in calcium oxalate endocytosis by Madin-Darby canine kidney cells
Provedor de dados: 56
Autores: Campos,A.H.; Schor,N.
Calcium oxalate (CaOx) crystals adhere to and are internalized by tubular renal cells and it seems that this interaction is related (positively or negatively) to the appearance of urinary calculi. The present study analyzes a series of mechanisms possibly involved in CaOx uptake by Madin-Darby canine kidney (MDCK) cells. CaOx crystals were added to MDCK cell cultures and endocytosis was evaluated by polarized light microscopy. This process was inhibited by an increase in intracellular calcium by means of ionomycin (100 nM; N = 6; 43.9% inhibition; P&amp;lt;0.001) or thapsigargin (1 µM; N = 6; 33.3% inhibition; P&amp;lt;0.005) administration, and via blockade of cytoskeleton assembly by the addition of colchicine (10 µM; N = 8; 46.1% inhibition;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium oxalate; DCK cells; Mechanisms of endocytosis; Renal stone.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100015

Dermal dendritic cell number correlates with serum autoantibody titers in Brazilian pemphigus foliaceus patients
Provedor de dados: 56
Autores: Chiossi,M.P.V.; Costa,R.S.; Roselino,A.M.F.
Pemphigus foliaceus (PF) is an autoimmune bullous disease endemic in Brazil. Since serum IL-12 is increased in patients with PF and Langerhans cells (LC) produce IL-12, we titrated serum autoantibodies by indirect immunofluorescence, and quantified epidermal dendritic cells, known as LC, and dermal dendritic cells (DC). Biopsies of blistering lesions were obtained from 22 patients, 13 of whom were submitted to biopsy of both injured and of apparently healthy skin. The control groups consisted of skin from 8 cadavers and from 12 women submitted to breast plastic surgery. LC and DC were identified with anti-CD1a antibody and quantified by morphometric analysis. LC number in the lesion and in apparently healthy skin from PF patients was similar to that of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anti-CD1a; Dendritic cells; Fogo selvagem; Langerhans cells; Pemphigus foliaceus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300008

Expression of the Mycobacterium bovis P36 gene in Mycobacterium smegmatis and the baculovirus/insect cell system
Provedor de dados: 56
Autores: Bigi,F.; Taboga,O.; Romano,M.I.; Alito,A.; Fisanotti,J.C.; Cataldi,A.A.
In the present study we evaluated different systems for the expression of mycobacterial antigen P36 secreted by Mycobacterium bovis. P36 was detected by Western blot using a specific antiserum. The P36 gene was initially expressed in E. coli, under the control of the T7 promoter, but severe proteolysis prevented its purification. We then tried to express P36 in M. smegmatis and insect cells. For M. smegmatis, we used three different plasmid vectors differing in copy number and in the presence of a promoter for expression of heterologous proteins. P36 was detected in the cell extract and culture supernatant in both expression systems and was recognized by sera from M. bovis-infected cattle. To compare the expression level and compartmentalization, the MPB70...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Mycobacterium smegmatis; Insect cells; Baculovirus; Secreted proteins; P36; MPB70; Antigen.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100004

Thalidomide and pentoxifylline block the renal effects of supernatants of macrophages activated with Crotalus durissus cascavella venom
Provedor de dados: 56
Autores: Martins,A.M.C.; Nobre,A.C.L.; Almeida,A.C.; Bezerra,G.; Lima,A.A.M.; Fonteles,M.C.; Monteiro,H.S.A.
Because thalidomide and pentoxifylline inhibit the synthesis and release of tumor necrosis factor-alpha (TNF-alpha), we determined the effect of these drugs on the renal damage induced by supernatants of macrophages activated with Crotalus durissus cascavella venom in order to identify the role of TNF-alpha in the process. Rat peritoneal macrophages were collected with RPMI medium and stimulated in vitro with C.d. cascavella venom (10 µg/ml) in the absence and presence of thalidomide (15 µM) or pentoxifylline (500 µM) for 1 h and washed and kept in culture for 2 h. Supernatant (1 ml) was tested on an isolated perfused rat kidney (N = 6 for each group). The first 30 min of each experiment were used as control. The supernatant was added to the perfusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nephrotoxicity; Crotalus durissus cascavella; Pentoxifylline; Thalidomide; Macrophage.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000012

Anti-ophidian activity of Bredemeyera floribunda Willd. (Polygalaceae) root extract on the local effects induced by Bothrops jararacussu venom
Provedor de dados: 56
Autores: Alves,N.T.Q.; Ximenes,R.M.; Jorge,R.J.B.; Silveira,J.A.M.; Santos,J.V.A.; Rodrigues,F.A.P.; Costa,P.H.S.; Xavier Jr.,F.A.F.; Evangelista,J.S.A.M.; Havt,A.; Soares,V.C.G.; Toyama,M.H.; Oliveira,A.N.A.; Araújo,R.M.; Alves,R.S.; Monteiro,H.S.A.
Bredemeyera floribunda roots are popularly used to treat snakebites in the semiarid region of Northeast Brazil, and previous studies indicate the anti-ophidian actions of triterpenoid saponins found in its roots. To assess B. floribunda root extract (BFRE) activity against the effects of Bothrops jararacussu venom (BjuV), antiphospholipasic, antiproteolytic, antihemorrhagic, antinecrotic, and anti-edematogenic activities were investigated in mice. Phytochemical analysis revealed the presence of saponins, flavonoids, and sugars, with rutin and saccharose being the major constituents of BFRE. Acute toxicity was determined and BFRE was nontoxic to mice. Phospholipase A2 and proteolytic activities induced by BjuV were inhibited in vitro by BFRE at all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bredemeyera floribunda; Snakeroot; Snake venom; Rutin; Antihemorrhagic; Anti-edematogenic.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100608

High frequency of mutation G377S in Brazilian type 3 Gaucher disease patients
Provedor de dados: 56
Autores: Rozenberg,R.; Araújo,F.T.; Fox,D.C.; Aranda,P.; Nonino,A.; Micheletti,C.; Martins,A.M.; Cravo,R.; Sobreira,E.; Pereira,L.V.
Gaucher disease (GD), the most prevalent lysosome storage disorder, presents an autosomal recessive mode of inheritance. It is a paradigm for therapeutic intervention in medical genetics due to the existence of effective enzyme replacement therapy. We report here the analysis of GD in 262 unrelated Brazilian patients, carried out in order to establish the frequency of the most common mutations and to provide prognostic information based on genotype-phenotype correlations. Among 247 type 1 GD patients, mutation N370S was detected in 47% of all the alleles, but N370S/N370S homozygosity was found in only 10% of the patients, a much lower frequency than expected, suggesting that most individuals presenting this genotype may not receive medical attention....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gaucher disease; GBA gene; Allele dose-effect.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900004

Hepatitis C virus infection, cryoglobulinemia, and peripheral neuropathy: a case report
Provedor de dados: 56
Autores: Vigani,A.G.; Macedo-de-Oliveira,A.; Pavan,M.H.P.; Pedro,M.N.; Gonçales Jr.,F.L.
Hepatitis C virus (HCV) is essentially hepatotropic but its manifestations can extend beyond the liver. It can be associated with autoimmune diseases, such as mixed cryoglobulinemia, membranoproliferative glomerulonephritis, autoimmune thyroiditis, and lymphoproliferative disorders. The mechanisms that trigger these manifestations are not completely understood. We describe a 48-year-old man with chronic HCV infection (circulating HCV RNA and moderate hepatitis as indicated by liver biopsy), cryoglobulinemia, and sensory and motor peripheral neuropathy. The diagnosis of multineuropathy was confirmed by clinical examination and electromyographic tests. A nerve biopsy revealed an inflammatory infiltrate in the perineurial space and signs of demyelination and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Hepatitis C virus; Neurological manifestations; Cryoglobulins; Interferon; Ribavirin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200001

Detection of human parvovirus B19 in a patient with hepatitis
Provedor de dados: 56
Autores: Pinho,J.R.R.; Alves,V.A.F.; Vieira,A.F.; Moralez,M.O.S.; Fonseca,L.E.P.; Guz,B.; Wakamatsu,A.; Cançado,E.L.R.; Carrilho,F.J.; da Silva,L.C.; Bernardini,A.P.; Durigon,E.L.
Parvovirus B19 has been associated by some investigators with cases of severe hepatitis. The aim of the present study was to determine the presence of active parvovirus B19 infection among 129 Brazilian patients with non-A-E hepatitis. The patients were assayed for antibodies against parvovirus B19, IgM class, by ELISA. In IgM-positive cases, parvovirus B19 DNA was assayed by PCR in serum and liver tissue and parvovirus VP1 antigen in liver tissue was assayed by immunohistochemistry. Antibodies against parvovirus B19, IgM class, were detected in 3 (2.3%) of 129 patients with non-A-E hepatitis. Previous surgery and blood transfusions were reported by these 3 patients. One patient was a 56-year-old female with severe hepatitis, with antimitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parvovirus B19; Hepatitis non-A-E; Autoimmune hepatitis; Polymerase chain reaction; Immunohistochemistry.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900005

Galectins: a key intersection between glycobiology and immunology
Provedor de dados: 56
Autores: Rabinovich,G.A.; Riera,C.M.; Landa,C.A.; Sotomayor,C.E.
Galectins are a family of evolutionarily conserved animal lectins, widely distributed from lower invertebrates to mammals. They share sequence and structure similarities in the carbohydrate recognition domain and specificity for polylactosamine-enriched glycoconjugates. In the last few years significant experimental data have been accumulated concerning their participation in different biological processes requiring carbohydrate recognition such as cell adhesion, cell growth regulation, inflammation, immunomodulation, apoptosis and metastasis. In the present review we will discuss some exciting questions and advances in galectin research, highlighting the significance of these proteins in immunological processes and their implications in biomedical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galectins; Immunomodulation; Apoptosis; Adhesion; Embryogenesis; Metastasis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400002

Neural reflex regulation of arterial pressure in pathophysiological conditions: interplay among the baroreflex, the cardiopulmonary reflexes and the chemoreflex
Provedor de dados: 56
The maintenance of arterial pressure at levels adequate to perfuse the tissues is a basic requirement for the constancy of the internal environment and survival. The objective of the present review was to provide information about the basic reflex mechanisms that are responsible for the moment-to-moment regulation of the cardiovascular system. We demonstrate that this control is largely provided by the action of arterial and non-arterial reflexes that detect and correct changes in arterial pressure (baroreflex), blood volume or chemical composition (mechano- and chemosensitive cardiopulmonary reflexes), and changes in blood-gas composition (chemoreceptor reflex). The importance of the integration of these cardiovascular reflexes is well understood and it...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Arterial pressure; Bezold-Jarisch reflex; Baroreflex; Chemoreflex.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400014

Attentional bias modulation by reappraisal in patients with generalized anxiety disorder: an event-related potential study
Provedor de dados: 56
Autores: Han,H.Y.; Gan,T.; Li,P.; Li,Z.J.; Guo,M.; Yao,S.M.
Affective states influence subsequent attention allocation. We evaluated emotional negativity bias modulation by reappraisal in patients with generalized anxiety disorder (GAD) relative to normal controls. Event-related potential (ERP) recordings were obtained, and changes in P200 and P300 amplitudes in response to negative or neutral words were noted after decreasing negative emotion or establishing a neutral condition. We found that in GAD patients only, the mean P200 amplitude after negative word presentation was much higher than after the presentation of neutral words. In normal controls, after downregulation of negative emotion, the mean P300 amplitude in response to negative words was much lower than after neutral words, and this was significant in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reappraisal; Generalized anxiety disorder; Event-related potential; P200; P300.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700576

Treadmill exercise testing of asymptomatic men and women without evidence of heart disease
Provedor de dados: 56
Autores: Chalela,W.A.; Fukushima,R.B.; Araujo,F.; Lima,A.C.P.; Moffa,P.J.; Mansur,A.J.
The aim of this study was to test the hypothesis of differences in performance including differences in ST-T wave changes between healthy men and women submitted to an exercise stress test. Two hundred (45.4%) men and 241 (54.6%) women (mean age: 38.7 ± 11.0 years) were submitted to an exercise stress test. Physiologic and electrocardiographic variables were compared by the Student t-test and the chi-square test. To test the hypothesis of differences in ST-segment changes, data were ranked with functional models based on weighted least squares. To evaluate the influence of gender and age on the diagnosis of ST-segment abnormality, a logistic model was adjusted; P &lt; 0.05 was considered to be significant. Rate-pressure product, duration of exercise and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ST-T wave changes; Exercise stress test; Asymptomatic; Women.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200018

Connections: can the 20th century coronary heart disease epidemic reveal something about the 1918 influenza lethality?
Provedor de dados: 56
Autores: Azambuja,M.I.
This essay proposes that the ecologic association shown between the 20th century coronary heart disease epidemic and the 1918 influenza pandemic could shed light on the mechanism associated with the high lethality of the latter. It suggests that an autoimmune interference at the apoB-LDL interface could explain both hypercholesterolemia and inflammation (through interference with the cellular metabolism of arachidonic acid). Autoimmune inflammation, then, would explain the 1950s-60s acute coronary events (coronary thrombosis upon influenza re-infection) and the respiratory failure seen among young adults in 1918. This hypothesis also argues that the lethality of the 1918 pandemic may have not depended so much on the 1918 virus as on an immune vulnerability...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Respiratory distress syndrome; Autoimmunity; Influenza; Coronary disease; Disease susceptibility; Disease outbreaks.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100001

Rat vas deferens SERCA2 is modulated by Ca2+/calmodulin protein kinase II-mediated phosphorylation
Provedor de dados: 56
Autores: Rodriguez,J.B.R.; Muzi-Filho,H.; Valverde,R.H.F.; Quintas,L.E.M.; Noel,F.; Einicker-Lamas,M.; Cunha,V.M.N.
Ca2+ pumps are important players in smooth muscle contraction. Nevertheless, little information is available about these pumps in the vas deferens. We have determined which subtype of sarco(endo)plasmic reticulum Ca2+-ATPase isoform (SERCA) is expressed in rat vas deferens (RVD) and its modulation by calmodulin (CaM)-dependent mechanisms. The thapsigargin-sensitive Ca2+-ATPase from a membrane fraction containing the highest SERCA levels in the RVD homogenate has the same molecular mass (∼115 kDa) as that of SERCA2 from the rat cerebellum. It has a very high affinity for Ca2+ (Ca0.5 = 780 nM) and a low sensitivity to vanadate (IC50 = 41 µM). These facts indicate that SERCA2 is present in the RVD. Immunoblotting for CaM and Ca2+/calmodulin-dependent protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+/calmodulin-dependent kinase II; Calcium homeostasis; Calmodulin; Rat vas deferens; SERCA2; Thapsigargin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300227

Toxic effects of mercury, lead and gadolinium on vascular reactivity
Provedor de dados: 56
Autores: Vassallo,D.V.; Simões,M.R.; Furieri,L.B.; Fioresi,M.; Fiorim,J.; Almeida,E.A.S.; Angeli,J.K.; Wiggers,G.A.; Peçanha,F.M.; Salaices,M.
Heavy metals have been used in a wide variety of human activities that have significantly increased both professional and environmental exposure. Unfortunately, disasters have highlighted the toxic effects of metals on different organs and systems. Over the last 50 years, the adverse effects of chronic lead, mercury and gadolinium exposure have been underscored. Mercury and lead induce hypertension in humans and animals, affecting endothelial function in addition to their other effects. Increased cardiovascular risk after exposure to metals has been reported, but the underlying mechanisms, mainly for short periods of time and at low concentrations, have not been well explored. The presence of other metals such as gadolinium has raised concerns about...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metal toxicity; Vascular reactivity; Mercury; Lead; Gadolinium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900016

Behavioral correlates of the activity of serotonergic and non-serotonergic neurons in caudal raphe nuclei
Provedor de dados: 56
Autores: Ribeiro-do-Valle,L.E.; Lucena,R.L.B.G.
We investigated the behavioral correlates of the activity of serotonergic and non-serotonergic neurons in the nucleus raphe pallidus (NRP) and nucleus raphe obscurus (NRO) of unanesthetized and unrestrained cats. The animals were implanted with electrodes for recording single unit activity, parietal oscillographic activity, and splenius, digastric and masseter electromyographic activities. They were tested along the waking-sleep cycle, during sensory stimulation and during drinking behavior. The discharge of the serotonergic neurons decreased progressively from quiet waking to slow wave sleep and to fast wave sleep. Ten different patterns of relative discharge across the three states were observed for the non-serotonergic neurons. Several non-serotonergic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleus raphe pallidus; Nucleus raphe obscurus; Waking-sleep cycle; Respiration; Startle behavior; Drinking behavior.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700012

Frequent occurrence of nicotinic acetylcholine receptors in GABAergic neurons of the chick visual system
Provedor de dados: 56
Autores: Torrão,A.S.; Britto,L.R.G.
Double-labeling immunohistochemical methods were used to investigate the occurrence of the alpha8 and alpha5 nicotinic receptor subunits in presumptive GABAergic neurons of the chick nervous system. Nicotinic receptor immunoreactivity was often found in cells exhibiting GABA-like immunoreactivity, especially in the visual system. The alpha8 subunit appeared to be present in presumptive GABAergic cells of the ventral lateral geniculate nucleus, nucleus of the basal optic root of the accessory optic system, and the optic tectum, among several other structures. The alpha5 subunit was also found in GABA-positive neurons, as observed in the lentiform nucleus of the mesencephalon and other pretectal nuclei. The numbers of alpha8- and alpha5-positive neurons that...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Acetylcholine; GABA; Neurotransmitters; Nicotinic receptors; Visual system.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000013

The brain decade in debate: I. Neurobiology of learning and memory
Provedor de dados: 56
Autores: Baddeley,A.; Bueno,O; Cahill,L.; Fuster,J.M.; Izquierdo,I.; McGaugh,J.L.; Morris,R.G.M.; Nadel,L.; Routtenberg,A.; Xavier,G.; Da Cunha,C.
This article is a transcription of an electronic symposium in which some active researchers were invited by the Brazilian Society for Neuroscience and Behavior (SBNeC) to discuss the last decade's advances in neurobiology of learning and memory. The way different parts of the brain are recruited during the storage of different kinds of memory (e.g., short-term vs long-term memory, declarative vs procedural memory) and even the property of these divisions were discussed. It was pointed out that the brain does not really store memories, but stores traces of information that are later used to create memories, not always expressing a completely veridical picture of the past experienced reality. To perform this process different parts of the brain act as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory; Learning; Hippocampus; Prefrontal; Cortex; Amygdala; Memory systems.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900002

Serum leptin concentration during puberty in healthy nonobese adolescents
Provedor de dados: 56
Autores: Brandão,C.M.A.; Lombardi,M.T.; Nishida,S.K.; Hauache,O.M.; Vieira,J.G.H.
Data obtained during the past five years have indicated that there are important age- and gender-based differences in the regulation and action of leptin in humans. To study the physiological changes of leptin during puberty in both sexes, and its relationship with body composition and sexual maturation, we measured leptin concentrations in 175 healthy adolescents (80 girls, 95 boys, 10-18 years of age), representing all pubertal stages. We excluded individuals with a body mass index (BMI) below the 5thor above the 95th percentile relative to age. Serum concentrations of leptin were determined by a monoclonal antibody-based immunofluorimetric assay, developed in our laboratory. Body composition was determined by dual-energy X-ray absorptiometry. Pubertal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leptin; Pubertal development; Body composition.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000003

Lichen planus with multiple system involvement including the mouth, vagina, urethra, and scalp: a case report
Provedor de dados: 56
Autores: Mi,Lan; Zhang,Hong; Zhang,Dai; Zhang,Miao.
This is a case report of lichen planus (LP) with multiple system involvement. A 35-year-old female patient was admitted in November 2014 with a 5-year history of painful/difficult sexual intercourse and loss of oral mucosa, and an 8-year history of focal hair loss. Earlier, the patient had been unable to adhere to corticosteroid therapy because of severe adverse side effects. In September 2014, labia minora mucosa defects and stricture of the urethral orifice (with dysuria), vaginal orifice, and vagina were identified. Biopsy was performed and a diagnosis of erosive LP was made. The patient was treated with an oral immunosuppressant (cyclosporine A) and urethral/vaginal dilatation. Urine flow rate and sex life were improved after 6 months and she...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Lichen planus; Intercourse pain; Multiple system involvement; Corticosteroid; Immunosuppressant; Case report.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000501

Effect of physical training on liver expression of activin A and follistatin in a nonalcoholic fatty liver disease model in rats
Provedor de dados: 56
Autores: Silva,R.N.; Bueno,P.G.; Avó,L.R.S.; Nonaka,K.O.; Selistre-Araújo,H.S.; Leal,A.M.O.
Nonalcoholic fatty liver disease (NAFLD) is characterized by fat accumulation in the liver and is associated with obesity and insulin resistance. Activin A is a member of the transforming growth factor beta (TGF)-β superfamily and inhibits hepatocyte growth. Follistatin antagonizes the biological actions of activin. Exercise is an important therapeutic strategy to reduce the metabolic effects of obesity. We evaluated the pattern of activin A and follistatin liver expression in obese rats subjected to swimming exercise. Control rats (C) and high-fat (HF) diet-fed rats were randomly assigned to a swimming training group (C-Swim and HF-Swim) or a sedentary group (C-Sed and HF-Sed). Activin βA subunit mRNA expression was significantly higher in HF-Swim than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activin A; Follistatin; Steatosis; Nonalcoholic fatty liver disease; Obesity; Exercise.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900746

What we know and do not know about women and kidney diseases; Questions unanswered and answers unquestioned: Reflection on World Kidney Day and International Woman’s Day
Provedor de dados: 56
Autores: Piccoli,G.B.; Al Rukhaimi,M.; Liu,Zhi-Hong; Zakharova,E.; Levin,A.
Chronic kidney disease affects approximately 10% of the world's adult population: it is within the top 20 causes of death worldwide, and its impact on patients and their families can be devastating. World Kidney Day and International Women's Day in 2018 coincide, thus offering an opportunity to reflect on the importance of women's health and specifically women’s kidney health on the community and the next generations, as well as to strive to be more curious about the unique aspects of kidney disease in women so that we may apply those learnings more broadly. Girls and women, who make up approximately 50% of the world's population, are important contributors to society and their families. Gender differences continue to exist around the world in access to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Women; Access to care; Kidney health; Acute and chronic kidney disease; Inequities.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700101

Glutamate receptors as seen by light: spectroscopic studies of structure-function relationships
Provedor de dados: 56
Autores: Mankiewicz,K.A.; Jayaraman,V.
Ionotropic glutamate receptors are major excitatory receptors in the central nervous system and also have a far reaching influence in other areas of the body. Their modular nature has allowed for the isolation of the ligand-binding domain and for subsequent structural studies using a variety of spectroscopic techniques. This review will discuss the role of specific ligand:protein interactions in mediating activation in the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid subtype of glutamate receptors as established by various spectroscopic investigations of the GluR2 and GluR4 subunits of this receptor. Specifically, this review will provide an introduction to the insight gained from X-ray crystallography and nuclear magnetic resonance investigations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vibrational spectroscopy; Fluorescence resonance energy transfer; A-Amino-3-hydroxy- 5-methyl-4- isoxazolepropionic acid receptors; Agonist activation; Ligand binding.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100001

Estragole blocks neuronal excitability by direct inhibition of Na+ channels
Provedor de dados: 56
Autores: Silva-Alves,K.S.; Ferreira-da-Silva,F.W.; Peixoto-Neves,D.; Viana-Cardoso,K.V.; Moreira-Júnior,L.; Oquendo,M.B.; Oliveira-Abreu,K.; Albuquerque,A.A.C.; Coelho-de-Souza,A.N.; Leal-Cardoso,J.H.
Estragole is a volatile terpenoid, which occurs naturally as a constituent of the essential oils of many plants. It has several pharmacological and biological activities. The objective of the present study was to investigate the mechanism of action of estragole on neuronal excitability. Intact and dissociated dorsal root ganglion neurons of rats were used to record action potential and Na+ currents with intracellular and patch-clamp techniques, respectively. Estragole blocked the generation of action potentials in cells with or without inflexions on their descendant (repolarization) phase (Ninf and N0 neurons, respectively) in a concentration-dependent manner. The resting potentials and input resistances of Ninf and N0 cells were not altered by estragole...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estragole; Local anesthetic; Essential oil; Dorsal root ganglia; Phenylpropanoid; Na+ current.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201056

Involvement of hippocampal NMDA receptors in retention of shuttle avoidance conditioning in rats
Provedor de dados: 56
Autores: Roesler,R.; Kuyven,C.R.; Kruel,A.V.S.; Quevedo,J.; Ferreira,M.B.C.
The purpose of this research was to evaluate the role of hippocampal N-methyl-D-aspartate (NMDA) receptors in acquisition and consolidation of memory during shuttle avoidance conditioning in rats. Adult male Wistar rats were surgically implanted with cannulae aimed at the CA1 area of the dorsal hippocampus. After recovery from surgery, animals were trained and tested in a shuttle avoidance apparatus (30 trials, 0.5-mA footshock, 24-h training-test interval). Immediately before or immediately after training, animals received a bilateral intrahippocampal 0.5-µl infusion containing 5.0 µg of the NMDA competitive receptor antagonist aminophosphonopentanoic acid (AP5) or vehicle (phosphate-buffered saline, pH 7.4). Infusion duration was 2 min per side....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: NMDA receptor; Hippocampus; Memory; Conditioning; Fear.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200014

Vasorelaxant effects of the potassium channel opener SR 47063 on the isolated human saphenous vein and rat aorta
Provedor de dados: 56
Autores: Criddle,D.N.; de Moura,R.S.
The vasorelaxant effects of SR 47063 (4-(2-cyanimino-1,2-dihydropyrid-1-yl)-2,2-dimethyl-6-nitrochromene), a new K+-channel opener structurally related to levcromakalim, were examined in isolated human saphenous vein (HSV) and rat aorta (RA). HSV or RA rings were precontracted with either KCl or noradrenaline and cumulative relaxant concentration-response curves were obtained for SR 47063 (0.1 nM to 1 µM) in the presence or absence of 3 µM glibenclamide. SR 47063 potently relaxed HSV and RA precontracted with 20 mM (but not 60 mM) KCl or 10 µM noradrenaline in a concentration-dependent manner, showing slightly greater activity in the aorta. The potency of the effect of SR 47063 on HSV and RA was 12- and 58-fold greater, respectively, than that reported for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SR 47063; K+-channel; Human saphenous vein; K ATP; Rat aorta; Levcromakalim; Glibenclamide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800013

Salvaged single-unit cord blood transplantation for 26 patients with hematologic malignancies not in remission
Provedor de dados: 56
Autores: Yao,W.; Zheng,C.C.; Liu,H.L.; Geng,L.Q.; Tang,B.L.; Tong,J.; Zhu,X.Y.; Song,K.D.; Qiang,P.; Sun,Z.M.
Treatments for patients with hematologic malignancies not in remission are limited, but a few clinical studies have investigated the effects of salvaged unrelated cord blood transplantation (CBT). We retrospectively studied 19 patients with acute leukemia, 5 with myelodysplastic syndrome (MDS with refractory anemia with excess blasts [RAEB]), and 2 with non-Hodgkin's lymphoma who received 1 CBT unit ≤2 loci human leukocyte antigen (HLA)-mismatched after undergoing myeloablative conditioning regimens between July 2005 and July 2014. All of them were in non-remission before transplantation. The infused total nucleated cell (TNC) dose was 4.07 (range 2.76-6.02)×107/kg and that of CD34+ stem cells was 2.08 (range 0.99-8.65)×105/kg. All patients were engrafted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Umbilical cord blood transplantation; Hematologic malignancies; Non-remission.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000002

[RETRACTED ARTICLE] Characteristics of liver fibrosis with different etiologies using a fully quantitative fibrosis assessment tool
Provedor de dados: 56
Autores: Wu,Q.; Zhao,X.; You,H.
This study aimed to test the diagnostic performance of a fully quantitative fibrosis assessment tool for liver fibrosis in patients with chronic hepatitis B (CHB), primary biliary cirrhosis (PBC) and non-alcoholic steatohepatitis (NASH). A total of 117 patients with liver fibrosis were included in this study, including 50 patients with CHB, 49 patients with PBC and 18 patients with NASH. All patients underwent liver biopsy (LB). Fibrosis stages were assessed by two experienced pathologists. Histopathological images of LB slices were processed by second harmonic generation (SHG)/two-photon excited fluorescence (TPEF) microscopy without staining, a system called qFibrosis (quantitative fibrosis) system. Altogether 101 quantitative features of the SHG/TPEF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver fibrosis; Quantitative assessment; Etiology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600703

Exercise stress testing before and after successful multivessel percutaneous transluminal coronary angioplasty
Provedor de dados: 56
Autores: Chalela,W.A.; Kreling,J.C.; Falcão,A.M.; Hueb,W.; Moffa,P.J.; Pereyra,P.L.A.; Ramires,J.A.F.
Controversy exists regarding the diagnostic accuracy, optimal technique, and timing of exercise testing after percutaneous coronary intervention. The objectives of the present study were to analyze variables and the power of exercise testing to predict restenosis or a new lesion, 6 months after the procedure. Eight-four coronary multi-artery diseased patients with preserved ventricular function were studied (66 males, mean age of all patients: 59 ± 10 years). All underwent coronary angiography and exercise testing with the Bruce protocol, before and 6 months after percutaneous coronary intervention. The following parameters were measured: heart rate, blood pressure, rate-pressure product (heart rate x systolic blood pressure), presence of angina, maximal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise test; Coronary artery disease; Percutaneous transluminal coronary angioplasty; Coronary multi-artery disease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400007

Interleukin-15 as a potential regulator of the innate immune response
Provedor de dados: 56
Autores: Carson,W.; Caligiuri,M.A.
Interleukin-15 (IL-15) is a newly-discovered cytokine that is produced by activated monocytes early in the course of the innate immune response. IL-15 is able to bind to components of the interleukin-2 receptor (IL-2R) despite the fact that it has no sequence homology with IL-2. IL-15 stimulates human natural killer cell proliferation, cytotoxicity, and cytokine production and can substitute for IL-2 under most conditions. In vitro studies indicate that monocyte-derived IL-15 may be an important determinant of IFN-gamma production by NK cells. In addition, IL-15 is able to promote the survival of natural killer cells under serum-free conditions. The IL-15 receptor is a heterotrimeric complex which is composed of the IL-2Rß and &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-15; Interleukin-12; Gamma interferon; Natural killer cell; Innate immunity.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100001

Analysis of different levels of positive end-expiratory pressure during lung retrieval for transplantation: an experimental study
Provedor de dados: 56
Autores: Gonçalves-Ferri,W.A.; Jauregui,A.; Martins-Celini,F.P.; Sansano,I.; Fabro,A.T.; Sacramento,E.M.F.; Aragon,D.C.; Ochoa,J.M.
Atelectasis and inadequate oxygenation in lung donors is a common problem during the retrieval of these organs. Nevertheless, the use of high positive end-expiratory pressure (PEEP) is not habitual during procedures of lung retrieval. Twenty-one Sprague-Dawley male consanguineous rats were used in the study. The animals were divided into 3 groups according to the level of PEEP used: low (2 cmH2O), moderate (5 cmH2O), and high (10 cmH2O). Animals were ventilated with a tidal volume of 6 mL/kg. Before lung removal, the lungs were inspected for the presence of atelectasis. When atelectasis was detected, alveolar recruitment maneuvers were performed. Blood gasometric analysis was performed immediately. Finally, the lungs were retrieved, weighed, and submitted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anesthesia; Lung transplantation; Mechanical ventilation; Pulmonary recruitment; PEEP.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700607

Diagnostic value of circulating lncRNA ANRIL and its correlation with coronary artery disease parameters
Provedor de dados: 56
Autores: Hu,Yao; Hu,Jing.
This study aimed to detect the expression of the long non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) and evaluate its correlation with disease risk, stenosis degree, inflammation, as well as overall survival (OS) in coronary artery disease (CAD) patients. A total of 230 patients who underwent diagnostic coronary angiography were consecutively recruited and assigned to CAD group (n=125) or control group (n=105) according to presence or absence of CAD. Gensini score was calculated to assess the severity of coronary artery damage. Plasma samples were collected and the expression ANRIL was detected in all participants. High-sensitivity C-reactive protein (hs-CRP), erythrocyte sedimentation rate (ESR), and cytokines including tumor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA ANRIL; Coronary artery disease; Diagnosis; Disease severity; Inflammatory responses; Overall survival.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800612

CorrectionBraz J Med Biol Res 2011; 44(8): 793-800Candidate gene linkage analysis indicates genetic heterogeneity in Marfan syndrome
Provedor de dados: 56
Autores: Teixeira,L.V.S.; Lezirovitz,K.; Pereira,L.V.; Perez,A.B.A.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000014

Early prediction of poor outcome in patients with acute asthma in the emergency room
Provedor de dados: 56
Autores: Mallmann,F.; Fernandes,A.K.; Ávila,E.M.; Nogueira,F.L.; Steinhorst,A.M.P.; Saucedo,D.Z.; Machado,F.J.; Raymundi,M.G.; Dalcin,P.T.R.; Menna Barreto,S.S.
Early identification of patients who need hospitalization or patients who should be discharged would be helpful for the management of acute asthma in the emergency room. The objective of the present study was to examine the clinical and pulmonary functional measures used during the first hour of assessment of acute asthma in the emergency room in order to predict the outcome. We evaluated 88 patients. The inclusion criteria were age between 12 and 55 years, forced expiratory volume in the first second below 50% of predicted value, and no history of chronic disease or pregnancy. After baseline evaluation, all patients were treated with 2.5 mg albuterol delivered by nebulization every 20 min in the first hour and 60 mg of intravenous methylprednisolone....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Outcome; Index; Prognosis; Emergency medicine.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100006

Computerized invasive measurement of time-dependent intraocular pressure
Provedor de dados: 56
Autores: Campos,T.V.O.; Jacobovitz,S.; Almeida,H.G.; Massensini,A.R.; Moraes,M.F.D.
Several methods have been described to measure intraocular pressure (IOP) in clinical and research situations. However, the measurement of time varying IOP with high accuracy, mainly in situations that alter corneal properties, has not been reported until now. The present report describes a computerized system capable of recording the transitory variability of IOP, which is sufficiently sensitive to reliably measure ocular pulse peak-to-peak values. We also describe its characteristics and discuss its applicability to research and clinical studies. The device consists of a pressure transducer, a signal conditioning unit and an analog-to-digital converter coupled to a video acquisition board. A modified Cairns trabeculectomy was performed in 9 Oryctolagus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glaucoma; Intraocular pressure measurement; Trabeculectomy; Signal processing; Computer-assisted measurement.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900013

The conveyor belt hypothesis for thymocyte migration: participation of adhesion and de-adhesion molecules
Provedor de dados: 56
Autores: Villa-Verde,D.M.S.; Calado,T.C.; Ocampo,J.S.P.; Silva-Monteiro,E.; Savino,W.
Thymocyte differentiation is the process by which bone marrow-derived precursors enter the thymus, proliferate, rearrange the genes and express the corresponding T cell receptors, and undergo positive and/or negative selection, ultimately yielding mature T cells that will represent the so-called T cell repertoire. This process occurs in the context of cell migration, whose cellular and molecular basis is still poorly understood. Kinetic studies favor the idea that these cells leave the organ in an ordered pattern, as if they were moving on a conveyor belt. We have recently proposed that extracellular matrix glycoproteins, such as fibronectin, laminin and type IV collagen, among others, produced by non-lymphoid cells both in the cortex and in the medulla,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thymus; Thymic nurse cells; Adhesion molecules; Extracellular matrix; Galectin-3.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500010

Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines
Provedor de dados: 56
Autores: Zong,L.; Yu,Q.H.; Du,Y.X.; Deng,X.M.
Studies have shown that edaravone may prevent liver injury. This study aimed to investigate the effects of edaravone on the liver injury induced by D-galactosamine (GalN) and lipopolysaccharide (LPS) in female BALB/c mice. Edaravone was injected into mice 30 min before and 4 h after GalN/LPS injection. The survival rate was determined within the first 24 h. Animals were killed 8 h after GalN/LPS injection, and liver injury was biochemically and histologically assessed. Hepatocyte apoptosis was measured by TUNEL staining; proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] in the liver were assayed by ELISA; expression of caspase-8 and caspase-3 proteins was detected by Western blot assay; and caspase-3 activity was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edaravone; Apoptosis; Endotoxin; Liver injury; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300231

Hypotrophy of conduit artery walls of the offspring of nitric oxide-defective rats
Provedor de dados: 56
Autores: Kristek,F.; Gerová,M.
The objective of the present study was to investigate the structure of the arterial walls of the offspring stemming from nitric oxide (NO)-defective hypertensive parents. The parents were treated with N G-nitro-L-arginine methyl ester (40 mg kg-1 day-1) for 5 weeks. Blood pressure was measured noninvasively in six 30-day-old rats and nine age-matched controls. The cardiovascular system was perfused with glutaraldehyde at 120 mmHg. The thoracic aorta and carotid artery were processed for electron microscopy, and geometry was determined by light microscopy. Endothelial cells, smooth muscle cells (SMC) and extracellular matrix (ECM) were determined by the point counting method in electron micrographs of the carotid artery. The blood pressure of experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artery; Nitric oxide; Offspring; Hypertension; Vascular smooth muscle; Extracellular matrix; Morphometry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400018

Expression and biological activity of two recombinant polypeptides related to subunit 1 of the interferon-a receptor
Provedor de dados: 56
Autores: Yoon,S.; Hirata,R.D.C.; Nguyen,N.Y.; Curi,R.; Russo,M.; Hirata,M.H.
Abnormal production of interferon alpha (IFN-a) has been found in certain autoimmune diseases and can be also observed after prolonged therapy with IFN-a. IFN-a can contribute to the pathogenesis of allograft rejection in bone marrow transplants. Therefore, the development of IFN-a inhibitors as a soluble receptor protein may be valuable for the therapeutic control of these diseases. We have expressed two polypeptides encoding amino acids 93-260 (P1) and 261-410 (P2) of the extracellular domain of subunit 1 of the interferon-a receptor (IFNAR 1-EC) in E. coli. The activities of the recombinant polypeptides and of their respective antibodies were evaluated using antiproliferative and antiviral assays. Expression of P1 and P2 polypeptides was achieved by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon alpha; Interferon-a receptor; Inhibitor of interferon-a; Autoimmune diseases; Graft-versus-host disease; Recombinant protein.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700007

Effect of magnesium ion on human osteoblast activity
Provedor de dados: 56
Autores: He,L.Y.; Zhang,X.M.; Liu,B.; Tian,Y.; Ma,W.H.
Magnesium, a promising biodegradable metal, has been reported in several studies to increase bone formation. Although there is some information regarding the concentrations of magnesium ions that affect bone remodeling at a cellular level, little is known about the effect of magnesium ions on cell gap junctions. Therefore, this study aimed to systematically investigate the effects of different concentrations of magnesium on bone cells, and further evaluate its effect on gap junctions of osteoblasts. Cultures of normal human osteoblasts were treated with magnesium ions at concentrations of 1, 2 and 3 mM, for 24, 48 and 72 h. The effects of magnesium ions on viability and function of normal human osteoblasts and on gap junction intercellular communication...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnesium; Osteoblast; Gap junction intercellular communication.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700604

Study of the effects of ß-myrcene on rat fertility and general reproductive performance
Provedor de dados: 56
Autores: Paumgartten,F.J.R.; De-Carvalho,R.R.; Souza,C.A.M.; Madi,K.; Chahoud,I.
ß-Myrcene (MYR) is a monoterpene found in the oils of a variety of aromatic plants including lemongrass, verbena, hop, bay, and others. MYR and essential oils containing this terpenoid compound are used in cosmetics, household products, and as flavoring food additives. This study was undertaken to investigate the effects of MYR on fertility and general reproductive performance in the rat. MYR (0, 100, 300 and 500 mg/kg) in peanut oil was given by gavage to male Wistar rats (15 per dose group) for 91 days prior to mating and during the mating period, as well as to females (45 per dose group) continuously for 21 days before mating, during mating and pregnancy, and throughout the period of lactation up to postnatal day 21. On day 21 of pregnancy one-third of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SS-myrcene; Monoterpenes; Essential oils; Reproductive toxicity; Fertility; Embryofetal toxicity; Postnatal development.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700012

Caspase dependence of the death of neonatal retinal ganglion cells induced by axon damage and induction of autophagy as a survival mechanism
Provedor de dados: 56
Autores: Sternberg,C.; Benchimol,M.; Linden,R.
We examined the degeneration of post-mitotic ganglion cells in ex-vivo neonatal retinal explants following axon damage. Ultrastructural features of both apoptosis and autophagy were detected. Degenerating cells reacted with antibodies specific for activated caspase-3 or -9, consistent with the presence of caspase activity. Furthermore, peptidic inhibitors of caspase-9, -6 or -3 prevented cell death (100 µM Ac-LEDH-CHO, 50 µM Ac-VEID-CHO and 10 µM Z-DEVD-fmk, respectively). Interestingly, inhibition of autophagy by 7-10 mM 3-methyl-adenine increased the rate of cell death. Immunohistochemistry data, caspase activation and caspase inhibition data suggest that axotomy of neonatal retinal ganglion cells triggers the intrinsic apoptotic pathway, which, in turn,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Autophagy; Caspases; Retina; Central nervous system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000005

Lesion of the subthalamic nucleus reverses motor deficits but not death of nigrostriatal dopaminergic neurons in a rat 6-hydroxydopamine-lesion model of Parkinson's disease
Provedor de dados: 56
Autores: Rizelio,V.; Szawka,R.E.; Xavier,L.L.; Achaval,M.; Rigon,P.; Saur,L.; Matheussi,F.; Delattre,A.M.; Anselmo-Franci,J.A.; Meneses,M.; Ferraz,A.C.
The objective of the present study was to determine whether lesion of the subthalamic nucleus (STN) promoted by N-methyl-D-aspartate (NMDA) would rescue nigrostriatal dopaminergic neurons after unilateral 6-hydroxydopamine (6-OHDA) injection into the medial forebrain bundle (MFB). Initially, 16 mg 6-OHDA (6-OHDA group) or vehicle (artificial cerebrospinal fluid - aCSF; Sham group) was infused into the right MFB of adult male Wistar rats. Fifteen days after surgery, the 6-OHDA and SHAM groups were randomly subdivided and received ipsilateral injection of either 60 mM NMDA or aCSF in the right STN. Additionally, a control group was not submitted to stereotaxic surgery. Five groups of rats were studied: 6-OHDA/NMDA, 6-OHDA/Sham, Sham/NMDA, Sham/Sham, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subthalamic nucleus; Parkinson's disease; Substantia nigra pars compacta; 6-Hydroxydopamine; Tyrosine hydroxylase immunohistochemistry.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100012

Differentiating origins of outflow tract ventricular arrhythmias: a comparison of three different electrocardiographic algorithms
Provedor de dados: 56
Autores: Jiao,Z.Y.; Li,Y.B.; Mao,J.; Liu,X.Y.; Yang,X.C.; Tan,C.; Chu,J.M.; Liu,X.P.
Our objective is to evaluate the accuracy of three algorithms in differentiating the origins of outflow tract ventricular arrhythmias (OTVAs). This study involved 110 consecutive patients with OTVAs for whom a standard 12-lead surface electrocardiogram (ECG) showed typical left bundle branch block morphology with an inferior axis. All the ECG tracings were retrospectively analyzed using the following three recently published ECG algorithms: 1) the transitional zone (TZ) index, 2) the V2 transition ratio, and 3) V2 R wave duration and R/S wave amplitude indices. Considering all patients, the V2 transition ratio had the highest sensitivity (92.3%), while the R wave duration and R/S wave amplitude indices in V2 had the highest specificity (93.9%). The latter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premature ventricular contraction; Ventricular tachycardia; Electrocardiogram; Radiofrequency catheter ablation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500605

Effect of selective angiotensin antagonists on the antidiuresis produced by angiotensin-(1-7) in water-loaded rats
Provedor de dados: 56
Autores: Baracho,N.C.V.; Simões-e-Silva,A.C.; Khosla,M.C.; Santos,R.A.S.
In the present study we evaluated the nature of angiotensin receptors involved in the antidiuretic effect of angiotensin-(1-7) (Ang-(1-7)) in water-loaded rats. Water diuresis was induced in male Wistar rats weighing 280 to 320 g by water load (5 ml/100 g body weight by gavage). Immediately after water load the rats were treated subcutaneously with (doses are per 100 g body weight): 1) vehicle (0.05 ml 0.9% NaCl); 2) graded doses of 20, 40 or 80 pmol Ang-(1-7); 3) 200 nmol Losartan; 4) 200 nmol Losartan combined with 40 pmol Ang-(1-7); 5) 1.1 or 4.4 nmol A-779; 6) 1.1 nmol A-779 combined with graded doses of 20, 40 or 80 pmol Ang-(1-7); 7) 4.4 nmol A-779 combined with graded doses of 20, 40 or 80 pmol Ang-(1-7); 8) 95 nmol CGP 42112A, or 9) 95 nmol CGP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Angiotensin receptors; Antidiuresis; Losartan; A-779; Ang-(1-7).
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900016

The chronic blockade of angiotensin I-converting enzyme eliminates the sex differences of serum cytokine levels of spontaneously hypertensive rats
Provedor de dados: 56
Autores: Dalpiaz,P.L.M.; Lamas,A.Z.; Caliman,I.F.; Medeiros,A.R.S.; Abreu,G.R.; Moysés,M.R.; Andrade,T.U.; Alves,M.F.; Carmona,A.K.; Bissoli,N.S.
Sex hormones modulate the action of both cytokines and the renin-angiotensin system. However, the effects of angiotensin I-converting enzyme (ACE) on the proinflammatory and anti-inflammatory cytokine levels in male and female spontaneously hypertensive rats (SHR) are unclear. We determined the relationship between ACE activity, cytokine levels and sex differences in SHR. Female (F) and male (M) SHR were divided into 4 experimental groups each (n = 7): sham + vehicle (SV), sham + enalapril (10 mg/kg body weight by gavage), castrated + vehicle, and castrated + enalapril. Treatment began 21 days after castration and continued for 30 days. Serum cytokine levels (ELISA) and ACE activity (fluorimetry) were measured. Male rats exhibited a higher serum ACE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SHR Enalapril Sexual dimorphism Angiotensin I-converting enzyme Cytokines IL-10; IL-6 and TNF-α.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200171

Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Andrade,M.K.N.; Machado,S.M.A.; Leite,M.L.; Saad,M.H.F.
Assuming that the IS6110-restriction fragment length polymorphism (RFLP) changes at a constant rate of 3.2 years, this methodology was applied to demonstrate, for the first time, variant patterns of Mycobacterium tuberculosis (MTB) in multiple isolates obtained at short time intervals from sputum and blood of an HIV+ patient with multiple admissions to the Emergency Room and to the multidrug-resistant tuberculosis (MDR-TB) Reference Center of a secondary-care hospital in Rio de Janeiro, Brazil. In sputum, the IS6110-RFLP appeared in isolates with two variant patterns with 10 and 13 IS6110 copies. However, blood presented only the pattern corresponding to 10 copies, suggesting compartmentalization. With regard to the exact match of 10 of 13 bands, this may...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; TB-MDR; TB-RFLP; Gene mutation; Fingerprint.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500006

Effects of single-dose atorvastatin on interleukin-6, interferon gamma, and myocardial no-reflow in a rabbit model of acute myocardial infarction and reperfusion
Provedor de dados: 56
Autores: Zhao,X.J.; Liu,X.L.; He,G.X.; Xu,H.P.
The mechanisms of statins relieving the no-reflow phenomenon and the effects of single-dose statins on it are not well known. This study sought to investigate the effects of inflammation on the no-reflow phenomenon in a rabbit model of acute myocardial infarction and reperfusion (AMI/R) and to evaluate the effects of single-dose atorvastatin on inflammation and myocardial no-reflow. Twenty-four New Zealand white male rabbits (5-6 months old) were randomized to three groups of eight: a sham-operated group, an AMI/R group, and an atorvastatin-treated group (10 mg/kg). Animals in the latter two groups were subjected to 4 h of coronary occlusion followed by 2 h of reperfusion. Serum levels of interleukin (IL)-6 were measured by enzyme-linked immunosorbent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; No-reflow phenomenon; Inflammation; Atorvastatin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300245

Dialysis water treated by reverse osmosis decreases the levels of C-reactive protein in uremic patients
Provedor de dados: 56
Autores: Thomé,F.S.; Senger,M.; Garcez,C.; Garcez,J.; Chemello,C.; Manfro,R.C.
Atherosclerosis is a major complication of chronic renal failure. Microinflammation is involved in atherogenesis and is associated with uremia and dialysis. The role of dialysate water contamination in inducing inflammation has been debated. Our aim was to study inflammatory markers in patients on chronic dialysis, before and 3 to 6 months after switching the water purification system from deionization to reverse osmosis. Patients had demographic, clinical and nutritional information collected and blood drawn for determination of albumin, ferritin, C-reactive protein (CRP), interleukin-6, and tumor necrosis factor-alpha in both situations. Acceptable levels of water purity were less than 200 colony-forming units of bacteria and less than 1 ng/ml of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemodialysis; C-reactive protein; Dialysate water; Reverse osmosi; Inflammation; Uremia.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500018

Protective effects of centrally acting sympathomodulatory drugs on myocardial ischemia induced by sympathetic overactivity in rabbits
Provedor de dados: 56
Autores: Catelli,M.; Feldman,J.; Bousquet,P.; Tibiriçá,E.
It is recognized that an imbalance of the autonomic nervous system is involved in the genesis of ventricular arrhythmia and sudden death during myocardial ischemia. In the present study we investigated the effects of clonidine and rilmenidine, two centrally acting sympathomodulatory drugs, on an experimental model of centrally induced sympathetic hyperactivity in pentobarbital-anesthetized New Zealand albino rabbits of either sex (2-3 kg, N = 89). We also compared the effects of clonidine and rilmenidine with those of propranolol, a ß-blocker, known to induce protective cardiovascular effects in patients with ischemic heart disease. Central sympathetic stimulation was achieved by intracerebroventricular injection of the excitatory amino acid L-glutamate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic nervous system; Antihypertensive agents; Antiarrhythmic agents; Ischemia; Sudden death.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100012

Identification of the molecular mechanisms associated with acute type A aortic dissection through bioinformatics methods
Provedor de dados: 56
Autores: Jiang,Tao; Si,Liangyi.
Aortic dissection is characterized by the redirection of blood flow, which flows through an intimal tear into the aortic media. The purpose of this study was to find potential acute type A aortic dissection (AAAD)-related genes and molecular mechanisms by bioinformatics. The gene expression profiles of GSE52093 were obtained from Gene Expression Omnibus (GEO) database, including 7 AAAD samples and 5 normal samples. The differentially expressed genes (DEGs) were detected between AAAD and normal samples. The functional annotation and pathway enrichment analysis were conducted through the Database for Annotation, Visualization and Integration Discovery (DAVID). A protein-protein interaction network was established by the Search Tool for the Retrieval of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aortic dissection; Differentially expressed genes; Functional enrichment analysis; Protein-protein interaction network; MicroRNAs; Small molecules.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100610

Dipyrone in association with atropine inhibits the effect on gastric emptying induced by hypoglycemia in rats
Provedor de dados: 56
Autores: Collares,E.F.; Vinagre,A.M.; Collares-Buzato,C.B.
Atropine (AT) and dipyrone (Dp) induce a delay of gastric emptying (GE) of liquids in rats by inhibiting muscarinic receptors and activating β2-adrenergic receptors, respectively. The objective of the present study was to determine the effects of pretreatment with AT and Dp, given alone or in combination, on the effect of hypoglycemia in the liquid GE in rats. Male Wistar adult rats (280-310 g) were pretreated intravenously with AT, Dp, AT plus Dp or their vehicle and then treated 30 min later with iv insulin or its vehicle (n=8-10 animals/group). Thirty min after treatment, GE was evaluated by determining, in awake rats, the percent gastric retention (%GR) of a saline meal labeled with phenol red administered by gavage. The results indicated that insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoglycemia; Gastric emptying; Dipyrone; Atropine; Muscarinic receptors; Adrenergic receptors.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000610

Glucagon-like peptide 1 improves insulin resistance in vitro through anti-inflammation of macrophages
Provedor de dados: 56
Autores: Guo,C.; Huang,T.; Chen,A.; Chen,X.; Wang,L.; Shen,F.; Gu,X.
Glucagon-like peptide 1 (GLP-1), a kind of gut hormone, is used in the treatment of type 2 diabetes (T2D). Emerging evidence indicates that GLP-1 has anti-inflammatory activity. Chronic inflammation in the adipose tissue of obese individuals is a cause of insulin resistance and T2D. We hypothesized that GLP-1 analogue therapy in patients with T2D could suppress the inflammatory response of macrophages, and therefore inhibit insulin resistance. Our results showed that GLP-1 agonist (exendin-4) not only attenuated macrophage infiltration, but also inhibited the macrophage secretion of inflammatory cytokines including TNF-β, IL-6, and IL-1β. Furthermore, we observed that lipopolysaccharide (LPS)-induced macrophage conditioned media could impair...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Glucagon-like peptide; Macrophage infiltration; Adipose inflammation; Insulin resistance.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200602

Comments on the paper "c-erbB-2 expression and nuclear pleomorphism in canine mammary tumors"
Provedor de dados: 56
Autores: Metze,K.; Adam,R.L.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Canine mammary tumors; Nuclear pleomorphism; Statistical analysis; Scientific methods; C-erbB-2 expression; MIB-1 expression.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200001

Long-term loss of color vision after exposure to mercury vapor
Provedor de dados: 56
Autores: Feitosa-Santana,C.; Costa,M.F.; Lago,M.; Ventura,D.F.
We evaluated the color vision of 24 subjects (41.6 ± 6.5 years; 6 females) who worked in fluorescent lamp industries. They had been occupationally exposed to mercury vapor (10.6 ± 5.2 years) and had been away from the source of exposure for 6.4 ± 4.04 years. Mean urinary concentration of mercury was 40.6 ± 36.4 µg/g creatinine during or up to 1 year after exposure and 2.71 ± 1.19 µg/g creatinine at the time of color vision testing or up to 1 year thereafter. All patients were diagnosed with chronic mercury intoxication, characterized by clinical symptoms and neuropsychological alterations. A control group (N = 36, 48.6 ± 11.9 years, 10 females, 1.5 ± 0.47 µg mercury/g creatinine) was subjected to the same tests. Inclusion criteria for both groups were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Color vision; Chromatic discrimination; Color discrimination; Psychophysics; Mercury intoxication; Occupational exposure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300017

Effect of tobacco smoke on hydrogen sulfide-induced rat thoracic aorta relaxation
Provedor de dados: 56
Autores: Zhang,H.T.; Zhang,T.; Chai,M.; Sun,J.J.; Yu,X.Y.; Liu,C.Z.; Huang,C.C.
Levels of hydrogen sulfide (H2S), a gaseous signaling molecule, are reduced in the serum of individuals who smoke. We hypothesized that tobacco smoke influenced smooth muscle relaxation by decreasing H2S levels and this effect could also influence expression of cystathionine γ-lyase (CSE) and sulfonylurea receptor-2 (SUR-2). The aim of this study was to explore the effect of tobacco smoke on H2S-mediated rat thoracic aorta relaxation and its possible mechanism. Thirty-two Sprague-Dawley rats were divided into four groups: control (C) group, short-term smoker (SS) group, mid-term smoker (MS) group, and long-term smoker (LS) group. H2S concentrations in serum, action of H2S on rat aortic vascular relaxation, and expression of CSE and SUR-2 in thoracic aortic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tobacco smoke; Hydrogen sulfide; Cardiac smooth muscle; Cystathionine-γ-lyase; Sulfonylurea receptor-2.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200602

Asthma: where is it going?
Provedor de dados: 56
Autores: Faffe,D.S.
Asthma is characterized by reversible airway obstruction, airway hyperresponsiveness, and airway inflammation. Although our understanding of its pathophysiological mechanisms continues to evolve, the relative contributions of airway hyperresponsiveness and inflammation are still debated. The first mechanism identified as important for asthma was bronchial hyperresponsiveness. In a second step, asthma was recognized also as an inflammatory disease, with chronic inflammation inducing structural changes or remodeling. However, persistence of airway dysfunction despite inflammatory control is observed in chronic severe asthma of both adults and children. More recently, a potential role for epithelial-mesenchymal communication or transition is emerging, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Epithelial-mesenchymal unit; Bronchial hyperresponsiveness; Remodeling.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900001

Ventricular performance and Na+-K+ ATPase activity are reduced early and late after myocardial infarction in rats
Provedor de dados: 56
Autores: Stefanon,I.; Cade,J.R.; Fernandes,A.A.; Ribeiro Junior,R.F.; Targueta,G.P.; Mill,J.G.; Vassallo,D.V.
Myocardial infarction leads to compensatory ventricular remodeling. Disturbances in myocardial contractility depend on the active transport of Ca2+ and Na+, which are regulated by Na+-K+ ATPase. Inappropriate regulation of Na+-K+ ATPase activity leads to excessive loss of K+ and gain of Na+ by the cell. We determined the participation of Na+-K+ ATPase in ventricular performance early and late after myocardial infarction. Wistar rats (8-10 per group) underwent left coronary artery ligation (infarcted, Inf) or sham-operation (Sham). Ventricular performance was measured at 3 and 30 days after surgery using the Langendorff technique. Left ventricular systolic pressure was obtained under different ventricular diastolic pressures and increased extracellular Ca2+...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Ouabain; Na+-K+ ATPase; Myocardial infarction; Calcium handling.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000005

miRNA expression profiles in chronic lymphocytic and acute lymphocytic leukemia
Provedor de dados: 56
Autores: Zanette,D.L.; Rivadavia,F.; Molfetta,G.A.; Barbuzano,F.G.; Proto-Siqueira,R.; Falcão,R.P.; Zago,M.A.; Silva-Jr,W.A.
MicroRNAs (miRNAs) are a class of small endogenous RNAs that play important regulatory roles by targeting mRNAs for cleavage or translational repression. miRNAs act in diverse biological processes including development, cell growth, apoptosis, and hematopoiesis, suggesting their association with cancer. We determined the miRNA expression profile of chronic and acute lymphocytic leukemias (CLL and ALL) using the TaqMan® MicroRNA Assays Human Panel (Applied Biosystems). Pooled leukemia samples were compared to pooled CD19+ samples from healthy individuals (calibrator) by the 2-DDCt method. Total RNA input was normalized based on the Ct values obtained for hsa-miR-30b. The five most highly expressed miRNAs were miR-128b, miR-204, miR-218, miR-331, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Non-coding RNA; MicroRNA; Hematopoietic malignancies; Leukemia; MiR-331.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100003

Male gonadal function, prolactin secretion and lactotroph population in an experimental model of cirrhosis
Provedor de dados: 56
Autores: Gonzales,P.H.; Rhoden,C.R.; Luz,C.; Corrêa,G.; Barbosa-Coutinho,L.M.; Oliveira,M.C.
Liver cirrhosis, a highly prevalent chronic disease, is frequently associated with endocrine dysfunctions, notably in the gonadal axis. We evaluated lactotroph population by immunohistochemistry, gonadotropins and prolactin by immunoradiometric assay and testosterone and estradiol by radioimmunoassay in adult male Wistar rats with cirrhosis induced by carbon tetrachloride. No significant difference in mean ± SEM percentages of lactotrophs was found between cirrhotic animals and controls (N = 12, mean 18.95 ± 1.29%). Although there was no significant difference between groups in mean serum levels of prolactin (control: 19.2 ± 4 ng/mL), luteinizing hormone (control: 1.58 ± 0.43 ng/mL), follicle-stimulating hormone (control: 19.11 ± 2.28 ng/mL), estradiol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypogonadism; Hyperestrogenemia; Hyperprolactinemia; Lactotrophs; Liver cirrhosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000011

Effects of caffeine on learning and memory in rats tested in the Morris water maze
Provedor de dados: 56
Autores: Angelucci,M.E.M.; Cesário,C.; Hiroi,R.H.; Rosalen,P.L.; Cunha,C. Da.
We studied some of the characteristics of the improving effect of the non-specific adenosine receptor antagonist, caffeine, using an animal model of learning and memory. Groups of 12 adult male Wistar rats receiving caffeine (0.3-30 mg/kg, ip, in 0.1 ml/100 g body weight) administered 30 min before training, immediately after training, or 30 min before the test session were tested in the spatial version of the Morris water maze task. Post-training administration of caffeine improved memory retention at the doses of 0.3-10 mg/kg (the rats swam up to 600 cm less to find the platform in the test session, P&lt;=0.05) but not at the dose of 30 mg/kg. Pre-test caffeine administration also caused a small increase in memory retrieval (the escape path of the rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory; Learning; Caffeine; Methylxanthines; Adenosine receptor antagonist; Morris water maze.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000013

Detection of specific IgE antibodies in parasite diseases
Provedor de dados: 56
Autores: Souza-Atta,M.L.B.; Araújo,M.I.; D'Oliveira Júnior,A.; Ribeiro-de-Jesus,A.; Almeida,R.P.; Atta,A.M.; Carvalho,E.M.
Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: IgE antibodies; Immunoassays; Schistosomiasis mansoni; Visceral leishmaniasis; Kala-azar.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008

Effects of piperitenone oxide on the intestinal smooth muscle of the guinea pig
Provedor de dados: 56
We investigated the effects of piperitenone oxide (PO), a major constituent of the essential oil of Mentha x villosa, on the guinea pig ileum. PO (30 to 740 µg/ml) relaxed basal tonus without significantly altering the resting membrane potential. In addition, PO relaxed preparations precontracted with either 60 mM K+ or 5 mM tetraethylammonium in a concentration-dependent manner. At concentrations from 0.1 to 10 µg/ml PO potentiated acetylcholine-induced contractions, while higher concentrations (&amp;gt;30 µg/ml) blocked this response. These higher PO concentrations also inhibited contractions induced by 60 mM K+. PO also blocked the components of acetylcholine contraction which are not sensitive to nifedipine or to solutions with nominal zero Ca2+ and...
Palavras-chave: Piperitenone oxide; Mentha x villosa; Ileum; Essential oil; Muscle relaxation; Acetylcholine; K+-induced contraction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600014

Effect of iron overload on the severity of liver histologic alterations and on the response to interferon and ribavirin therapy of patients with hepatitis C infection
Provedor de dados: 56
Autores: Souza,R.M.; Freitas,L.A.R.; Lyra,A.C.; Moraes,C.F.; Braga,E.L.; Lyra,L.G.C.
The objective of the present study was to determine the presence of hepatic iron overload in patients with chronic HCV infection and to correlate it with histologic alterations, HCV genotype and response to therapy. Liver tissue samples from 95 patients with chronic hepatitis C were divided into two groups: group I, presence of iron overload in hepatic tissue (Perls' staining) and group II, no iron overload. Hepatic iron overload was detected in 30 (31.6%) of 95 patients. Of the 69 patients tested by genotyping, 49 (71.01%) were genotype 1 and 20 (28.99%) genotype non-1. Iron overload was detected in 14 (28.6%) patients with genotype 1 and in 6 (30%) with genotype non-1 (P = 0.906). There was a significant difference in fibrosis stage between groups (P =...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis C infection; Iron overload; Genotype; Inflammatory activity; Fibrosis; Antiviral therapy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100009

The safety and efficacy of tPA intravenous thrombolysis for treating acute ischemic stroke patients with a history of cerebral hemorrhage
Provedor de dados: 56
Autores: Zhao,Guang-jian; Wang,Zi-ran; Lin,Fan-zhen; Cui,Yan-sen; Xu,Shun-liang.
Alteplase (tPA) intravenous thrombolysis is an effective treatment for acute ischemic stroke (AIS) when administered within 4.5 h of initial stroke symptoms. Here, its safety and efficacy were evaluated among AIS patients with a previous history of cerebral hemorrhage. Patients who arrived at the hospital within 4.5 h of initial stroke symptoms and who were treated with tPA intravenous thrombolysis or conventional therapies were analyzed. The 90-day modified Rankin scale (90-d mRS) was used alongside mortality and incidence of symptomatic intracerebral hemorrhage (SICH) rates to evaluate the curative effect of these therapies. Among 1,694 AIS patients, 805 patients were treated with intravenous thrombolysis, including patients with (n=793) or without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TPA; Acute ischemic stroke; History of cerebral hemorrhage; Intravenous thrombolysis; Contraindication.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200607

Correction Braz J Med Biol Res 1994; 27(11): 2667-2679 - Analysis of right and left ventricular performance of the rat heart with chronic myocardial infarction
Provedor de dados: 56
Autores: Stefanon,I.; Auxiliadora-Martins,M.; Vassallo,D.V.; Mill,J.G.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900015

Dose-dependent effects of 17-ß-estradiol on pituitary thyrotropin content and secretion in vitro
Provedor de dados: 56
Autores: Moreira,R.M.; Lisboa,P.C.; Curty,F.H.; Pazos-Moura,C.C.
We studied the basal and thyrotropin-releasing hormone (TRH) (50 nM) induced thyrotropin (TSH) release in isolated hemipituitaries of ovariectomized rats treated with near-physiological or high doses of 17-ß-estradiol benzoate (EB; sc, daily for 10 days) or with vehicle (untreated control rats, OVX). One group was sham-operated (normal control). The anterior pituitary glands were incubated in Krebs-Ringer bicarbonate medium, pH 7.4, at 37oC in an atmosphere of 95% O2/5% CO2. Medium and pituitary TSH was measured by specific RIA (NIDDK-RP-3). Ovariectomy induced a decrease (P&amp;lt;0.05) in basal TSH release (normal control = 44.1 ± 7.2; OVX = 14.7 ± 3.0 ng/ml) and tended to reduce TRH-stimulated TSH release (normal control = 33.0 ± 8.1; OVX = 16.6 ± 2.4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyrotropin (TSH); Ovariectomized rats; 17-ß-estradiol benzoate; Thyrotropin-releasing hormone (TRH).
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900012

Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a
Provedor de dados: 56
Autores: Fang,M.; Jin,A.; Zhao,Y.; Liu,X.
High plasma levels of homocysteine (Hcy) promote the progression of neurodegenerative diseases. However, the mechanism by which Hcy mediates neurotoxicity has not been elucidated. We observed that upon incubation with Hcy, the viability of a neuroblastoma cell line Neuro2a declined in a dose-dependent manner, and apoptosis was induced within 48 h. The median effective concentration (EC50) of Hcy was approximately 5 mM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) nuclear translocation and acylation has been implicated in the regulation of apoptosis. We found that nuclear translocation and acetylation of GAPDH increased in the presence of 5 mM Hcy and that higher levels of acetyltransferase p300/CBP were detected in Neuro2a cells. These findings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homocysteine; Glyceraldehyde-3-phosphate dehydrogenase; Apoptosis; Acetylate; P300/CBP.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200606

Biosynthesis and metabolism of steroid hormones by human adrenal carcinomas
Provedor de dados: 56
Autores: Brown,J.W.; Fishman,L.M.
Over a 15-year period, our university-based laboratory obtained 125 adrenal tumors, of which 15 (12%) were adrenal cortical carcinomas. Of these, 6 (40% of the carcinomas) occurred in patients with clear clinical manifestations of steroid hormone excess. Adrenal cortical carcinoma cells derived from the surgically resected tumors in 4 of these patients were isolated and established in primary culture. Radiotracer steroid interconversion studies were carried out with these cultures and also on mitochondria isolated from homogenized tissues. Large tumors had the lowest steroidogenic activities per weight, whereas small tumors had more moderately depressed enzyme activities relative to cells from normal glands. In incubations with pregnenolone as substrate, 1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenal carcinoma; Cell culture; Steroids; Metyrapone; Dibutyryl cAMP; ACTH; Krebs cycle intermediates.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000014

Activation of a P2Y4-like purinoceptor triggers an increase in cytosolic [Ca2+] in the red blood cells of the lizard Ameiva ameiva (Squamata, Teiidae)
Provedor de dados: 56
Autores: Sartorello,R.; Garcia,C.R.S.
An increasing number of pathophysiological roles for purinoceptors are emerging, some of which have therapeutic potential. Erythrocytes are an important source of purines, which can be released under physiological and physiopathological conditions, acting on purinergic receptors associated with the same cell or with neighboring cells. Few studies have been conducted on lizards, and have been limited to ATP agonist itself. We have previously shown that the red blood cells (RBCs) of the lizard Ameiva ameiva store Ca2+ in the endoplasmic reticulum (ER) and that the purinergic agonist ATP triggers a rapid and transient increase of [Ca2+]c by mobilization of the cation from internal stores. We also reported the ability of the second messenger IP3 to discharge...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red blood cells; Calcium; Teiidae; Ameiva ameiva; Purinoceptor; UTP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100002

Propofol pretreatment attenuates lipopolysaccharide-induced acute lung injury in rats by activating the phosphoinositide-3-kinase/Akt pathway
Provedor de dados: 56
Autores: Zhao,L.L.; Hu,G.C.; Zhu,S.S.; Li,J.F.; Liu,G.J.
The aim of this study was to investigate the effect of propofol pretreatment on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the role of the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathway in this procedure. Survival was determined 48 h after LPS injection. At 1 h after LPS challenge, the lung wet- to dry-weight ratio was examined, and concentrations of protein, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method or ELISA. Lung injury was assayed via lung histological examination. PI3K and p-Akt expression levels in the lung tissue were determined by Western blotting. Propofol pretreatment prolonged survival, decreased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lung injury; Propofol; Lipopolysaccharide; PI3K/Akt pathway.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201062

Late emergence of A594V and L595W mutations related to ganciclovir resistance in a patient with HCMV retinitis and long-term HIV progression
Provedor de dados: 56
Autores: Slavov,S.N.; Vilar,F.C.; Wagatsuma,V.M.D.; Santana,R.C.; Machado,A.A.; Fonseca,B.A.L. da; Kashima,S.; Covas,D.T.
The emergence of ganciclovir (GCV) resistance during the treatment of human cytomegalovirus (HCMV) infection is a serious clinical challenge, and is associated with high morbidity and mortality. In this case report, we describe the emergence of two consecutive mutations (A594V and L595W) related to GCV resistance in a patient with HCMV retinitis and long-term HIV progression after approximately 240 days of GCV use. Following the diagnosis of retinitis, the introduction of GCV did not result in viral load reduction. The detected mutations appeared late in the treatment, and we propose that other factors (high initial HCMV load, previous GCV exposure, low CD4+ cell count), in addition to the presence of resistance mutations, may have contributed to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human cytomegalovirus; HCMV; Ganciclovir; Resistance; AIDS.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900777

Role of non-coding RNAs in non-aging-related neurological disorders
Provedor de dados: 56
Autores: Vieira,A.S.; Dogini,D.B.; Lopes-Cendes,I.
Protein coding sequences represent only 2% of the human genome. Recent advances have demonstrated that a significant portion of the genome is actively transcribed as non-coding RNA molecules. These non-coding RNAs are emerging as key players in the regulation of biological processes, and act as "fine-tuners" of gene expression. Neurological disorders are caused by a wide range of genetic mutations, epigenetic and environmental factors, and the exact pathophysiology of many of these conditions is still unknown. It is currently recognized that dysregulations in the expression of non-coding RNAs are present in many neurological disorders and may be relevant in the mechanisms leading to disease. In addition, circulating non-coding RNAs are emerging as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA; Gene regulation; Molecular biomarkers.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800301

Low-dose doxycycline prevents inflammatory bone resorption in rats
Provedor de dados: 56
Autores: Bezerra,M.M.; Brito,G.A.C.; Ribeiro,R.A.; Rocha,F.A.C.
Matrix metalloproteinases (MMP) are considered to be key initiators of collagen degradation, thus contributing to bone resorption in inflammatory diseases. We determined whether subantimicrobial doses of doxycycline (DX) (&lt;=10 mg kg-1 day-1), a known MMP inhibitor, could inhibit bone resorption in an experimental periodontitis model. Thirty male Wistar rats (180-200 g) were subjected to placement of a nylon thread ligature around the maxillary molars and sacrificed after 7 days. Alveolar bone loss (ABL) was measured macroscopically in one hemiarcade and the contralateral hemiarcade was processed for histopathologic analysis. Groups of six animals each were treated with DX (2.5, 5 or 10 mg kg-1 day-1, sc, 7 days) and compared to nontreated (NT) rats. NT...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tetracyclines; Doxycycline; Metalloproteinases; Periodontitis; Osteoporosis; Bone resorption; Inflammation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500015

Factors associated with bacteremia due to multidrug-resistant Gram-negative bacilli in hematopoietic stem cell transplant recipients
Provedor de dados: 56
Autores: Garnica,M.; Maiolino,A.; Nucci,M.
The epidemiology of bacteremia developing during neutropenia has changed in the past decade, with the re-emergence of Gram-negative (GN) bacteria and the development of multidrug resistance (MDR) among GN bacteria. We conducted a case-control study in order to identify factors associated with bacteremia due to multidrug-resistant Gram-negative (MDRGN) isolates in hematopoietic stem cell transplant recipients. Ten patients with MDRGN bacteremia were compared with 44 patients with GN bacteremia without MDR. Bacteremia due to Burkholderia or Stenotrophomonas sp was excluded from analysis (3 cases), because the possibility of intrinsical resistance. Infection due to MDRGN bacteria occurred in 2.9% of 342 hematopoietic stem cell transplant recipients....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hematopoietic stem cell transplant; Neutropenia; Gram-negative bacteria; Antibiotic resistance; Risk factors.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300010

Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway
Provedor de dados: 56
Autores: Oliveira,I.C.; Brenner,E.; Chiu,J.; Hsieh,M.-H.; Kouranov,A.; Lam,H.-M.; Shin,M.J.; Coruzzi,G.
We are using molecular, biochemical, and genetic approaches to study the structural and regulatory genes controlling the assimilation of inorganic nitrogen into the amino acids glutamine, glutamate, aspartate and asparagine. These amino acids serve as the principal nitrogen-transport amino acids in most crop and higher plants including Arabidopsis thaliana. We have begun to investigate the regulatory mechanisms controlling nitrogen assimilation into these amino acids in plants using molecular and genetic approaches in Arabidopsis. The synthesis of the amide amino acids glutamine and asparagine is subject to tight regulation in response to environmental factors such as light and to metabolic factors such as sucrose and amino acids. For instance, light...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Carbon; Ammonium; Nitrogen assimilation; Gene expression; Arabidopsis thaliana.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500003

Fluorescent ligands for studying neuropeptide receptors by confocal microscopy
Provedor de dados: 56
Autores: Beaudet,A.; Nouel,D.; Stroh,T.; Vandenbulcke,F.; Dal-Farra,C.; Vincent,J.-P.
This paper reviews the use of confocal microscopy as it pertains to the identification of G-protein coupled receptors and the study of their dynamic properties in cell cultures and in mammalian brain following their tagging with specific fluorescent ligands. Principles that should guide the choice of suitable ligands and fluorophores are discussed. Examples are provided from the work carried out in the authors' laboratory using custom synthetized fluoresceinylated or BODIPY-tagged bioactive peptides. The results show that confocal microscopic detection of specifically bound fluorescent ligands permits high resolution appraisal of neuropeptide receptor distribution both in cell culture and in brain sections. Within the framework of time course experiments,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurotensin; Somatostatin; Opioids; Internalization; Endocytosis; Confocal microscopy.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100017

Effects of age and physical activity on the autonomic control of heart rate in healthy men
Provedor de dados: 56
Autores: Melo,R.C.; Santos,M.D.B.; Silva,E.; Quitério,R.J.; Moreno,M.A.; Reis,M.S.; Verzola,I.A.; Oliveira,L.; Martins,L.E.B.; Gallo-Junior,L.; Catai,A.M.
The effects of the aging process and an active life-style on the autonomic control of heart rate (HR) were investigated in nine young sedentary (YS, 23 ± 2.4 years), 16 young active (YA, 22 ± 2.1 years), 8 older sedentary (OS, 63 ± 2.4 years) and 8 older active (OA, 61 ± 1.1 years) healthy men. Electrocardiogram was continuously recorded for 15 min at rest and for 4 min in the deep breathing test, with a breath rate of 5 to 6 cycles/min in the supine position. Resting HR and RR intervals were analyzed by time (RMSSD index) and frequency domain methods. The power spectral components are reported in normalized units (nu) at low (LF) and high (HF) frequency, and as the LF/HF ratio. The deep breathing test was analyzed by the respiratory sinus arrhythmia...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Heart rate variability; Respiratory sinus arrhythmia; Autonomic nervous system; Aging process; Aerobic exercise.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900007

Cardiovascular actions of angiotensin-(1-7)
Provedor de dados: 56
Autores: Ferreira,A.J.; Santos,R.A.S.
Angiotensin-(1-7) (Ang-(1-7)) is now considered to be a biologically active member of the renin-angiotensin system. The functions of Ang-(1-7) are often opposite to those attributed to the main effector component of the renin-angiotensin system, Ang II. Chronic administration of angiotensin-converting enzyme inhibitors (ACEI) increases 10- to 25-fold the plasma levels of this peptide, suggesting that part of the beneficial effects of ACEI could be mediated by Ang-(1-7). Ang-(1-7) can be formed from Ang II or directly from Ang I. Other enzymatic pathways for Ang-(1-7) generation have been recently described involving the novel ACE homologue ACE2. This enzyme can form Ang-(1-7) from Ang II or less efficiently by the hydrolysis of Ang I to Ang-(1-9) with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin-(1-7) receptor Mas; Renin-angiotensin system; Cardiac function; Blood vessels; Angiotensin-converting enzyme 2; Transgenic models.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400003

Compound 48/80, a histamine-depleting agent, blocks the protective effect of morphine against electroconvulsive shock in mice
Provedor de dados: 56
Autores: Karadag,C.H.; Dokmeci,D.; Dost,T.; Ulugol,A.; Dokmeci,I.
We have shown that morphine has an anticonvulsive effect against maximal electroconvulsive shock (MES) in mice, and this effect is antagonized by histamine H1-receptor antagonists. Brain histamine is localized both in neurons and in mast cells, and morphine is known to enhance the turnover of neuronal histamine and to release histamine from mast cells. In the present experiments, compound 48/80 was injected chronically (0.5 mg/kg on day 1, 1 mg/kg on day 2, 2 mg/kg on day 3, 3 mg/kg on day 4, and 4 mg/kg on day 5, twice daily, ip) to deplete mast cell contents. Morphine (0.001-10 mg/kg, ip; N = 20) produced a dose-dependent anticonvulsive effect against MES seizure in mice with non-depleted mast cells, whereas it did not exert any anticonvulsive effect in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphine; Anticonvulsive effect; Histamine; Mast cells; Compound 48/80; Electroshock.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300011

Vascular adaptive responses to physical exercise and to stress are affected differently by nandrolone administration
Provedor de dados: 56
Autores: Bruder-Nascimento,T.; Cordellini,S.
Androgenic anabolic steroid, physical exercise and stress induce cardiovascular adaptations including increased endothelial function. The present study investigated the effects of these conditions alone and in combination on the vascular responses of male Wistar rats. Exercise was started at 8 weeks of life (60-min swimming sessions 5 days per week for 8 weeks, while carrying a 5% body-weight load). One group received nandrolone (5 mg/kg, twice per week for 8 weeks, im). Acute immobilization stress (2 h) was induced immediately before the experimental protocol. Curves for noradrenaline were obtained for thoracic aorta, with and without endothelium from sedentary and trained rats, submitted or not to stress, treated or not with nandrolone. None of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress; Physical exercise; Nandrolone; Vascular reactivity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400011

Establishment of a rat model for uterine leiomyomas based on Western and traditional Chinese medicine theories
Provedor de dados: 56
Autores: Zhao,Hui; Li,Yao; Xu,QiuXia; Peng,Fu; Zhao,JinShuang; Webb,R. Clinton; Peng,Cheng; Yu,ChengHao.
Uterine leiomyomas (ULs) are benign monoclonal tumors that arise from the underlying myometrial tissue in the uterus. Effective therapies are still lacking because of poor understanding of the pathophysiology and epidemiology. Hence, it is urgent to establish efficient animal models to screen novel anti-UL therapies. In this study, for the first time, traditional Chinese medicine and Western medicine were combined to establish an animal model of ULs in rats. In order to evaluate the function and value of the novel model, it was compared with other models. The long-term and short-term rat models for ULs were established using progesterone and diethylstilbestrol. Rats in Qi stagnation and blood stasis group were injected with epinephrine hydrochloride and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uterine leiomyomas; Animal model; Traditional Chinese medicine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900603

A carbohydrate-based mechanism of species recognition in sea urchin fertilization
Provedor de dados: 56
Autores: Mourão,P.A.S.
In the present review, we describe a systematic study of the sulfated polysaccharides from marine invertebrates, which led to the discovery of a carbohydrate-based mechanism of sperm-egg recognition during sea urchin fertilization. We have described unique polymers present in these organisms, especially sulfated fucose-rich compounds found in the egg jelly coat of sea urchins. The polysaccharides have simple, linear structures consisting of repeating units of oligosaccharides. They differ among the various species of sea urchins in specific patterns of sulfation and/or position of the glycosidic linkage within their repeating units. These polysaccharides show species specificity in inducing the acrosome reaction in sea urchin sperm, providing a clear-cut...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrosome reaction; Fertilization; Sulfated fucans; Glycosaminoglycans; Sulfated galactans; Species diversity; Sperm-egg recognition.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100002

Adrenomedullin and adrenotensin regulate collagen synthesis and proliferation in pulmonary arterial smooth muscle cells
Provedor de dados: 56
Autores: Li,W.; Kong,Q.Y.; Zhao,C.F.; Zhao,F.; Li,F.H.; Xia,W.; Wang,R.; Hu,Y.M.; Hua,M.
To understand the pathophysiological mechanisms of pulmonary arterial smooth muscle cell (PASMC) proliferation and extracellular-matrix accumulation in the development of pulmonary hypertension and remodeling, this study determined the effects of different doses of adrenomedullin (ADM) and adrenotensin (ADT) on PASMC proliferation and collagen synthesis. The objective was to investigate whether extracellular signal-regulated kinase (ERK1/2) signaling was involved in ADM- and ADT-stimulated proliferation of PASMCs in 4-week-old male Wistar rats (body weight: 100-150 g, n=10). The proliferation of PASMCs was examined by 5-bromo-2-deoxyuridine incorporation. A cell growth curve was generated by the Cell Counting Kit-8 method. Expression of collagen I,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenomedullin; Adrenotensin; Pulmonary arterial smooth muscle cell; Extracellular signal-regulated kinase-1; Collagen.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201047

Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs
Provedor de dados: 56
Autores: Mejia,J.; Reis,M.A.; Miranda,A.C.C.; Batista,I.R.; Barboza,M.R.F.; Shih,M.C.; Fu,G.; Chen,C.T.; Meng,L.J.; Bressan,R.A.; Amaro Jr,E.
The single photon emission microscope (SPEM) is an instrument developed to obtain high spatial resolution single photon emission computed tomography (SPECT) images of small structures inside the mouse brain. SPEM consists of two independent imaging devices, which combine a multipinhole collimator, a high-resolution, thallium-doped cesium iodide [CsI(Tl)] columnar scintillator, a demagnifying/intensifier tube, and an electron-multiplying charge-coupling device (CCD). Collimators have 300- and 450-µm diameter pinholes on tungsten slabs, in hexagonal arrays of 19 and 7 holes. Projection data are acquired in a photon-counting strategy, where CCD frames are stored at 50 frames per second, with a radius of rotation of 35 mm and magnification factor of one. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tomography; Preclinical imaging; Molecular imaging; Mice.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100936

Personality traits and psychiatric comorbidities in alcohol dependence
Provedor de dados: 56
Autores: Donadon,M.F.; Osório,F.L.
Non-adaptive personality traits may constitute risk factors for development of psychiatric disorders such as depression and anxiety. We aim to evaluate associations and the predictive value of personality traits among alcohol-dependent individuals, with or without psychiatric comorbidities. The convenience sample comprised two groups of males over 18 years of age: one with subjects who had an alcohol dependence diagnosis (AG, n=110), and a control group without abuse and/or alcohol dependence diagnosis (CG, n=110). The groups were assessed by means of the Structured Clinical Interview DSM-IV (SCID-IV). AG participants were recruited among outpatients from the university hospital, whereas CG participants were recruited from a primary healthcare program....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholism; Dependence; Personality traits; Comorbidity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100705

Association between elevated adiponectin level and adverse outcomes in patients with heart failure: a systematic review and meta-analysis
Provedor de dados: 56
Autores: Bai,Wenwei; Huang,Jingjing; Zhu,Min; Liu,Xiaoyong; Tao,Jianping.
Studies regarding the prognostic value of circulating adiponectin level in patients with heart failure are conflicting. The aim of this meta-analysis was to evaluate the association between elevated circulating adiponectin level and adverse outcomes in patients with heart failure. We searched PubMed and Embase databases from their inception to June 2018. Original observational studies that investigated the prognostic value of adiponectin in heart failure patients and reported all-cause mortality or combined endpoints of death/readmission as outcome measure were included. Pooled risk ratio (RR) with 95% confidence intervals (CI) were estimated by higher versus lower circulating adiponectin level. A total of 7 studies involving 862 heart failure patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adiponectin; Heart failure; Mortality; Readmission; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700610

Cytotoxicity and genotoxicity of low doses of mercury chloride and methylmercury chloride on human lymphocytes in vitro
Provedor de dados: 56
Autores: Silva-Pereira,L.C.; Cardoso,P.C.S.; Leite,D.S.; Bahia,M.O.; Bastos,W.R.; Smith,M.A.C.; Burbano,R.R.
Mercury is a xenobiotic metal that is a highly deleterious environmental pollutant. The biotransformation of mercury chloride (HgCl2) into methylmercury chloride (CH3HgCl) in aquatic environments is well-known and humans are exposed by consumption of contaminated fish, shellfish and algae. The objective of the present study was to determine the changes induced in vitro by two mercury compounds (HgCl2 and CH3HgCl) in cultured human lymphocytes. Short-term human leukocyte cultures from 10 healthy donors (5 females and 5 males) were set-up by adding drops of whole blood in complete medium. Cultures were separately and simultaneously treated with low doses (0.1 to 1000 µg/l) of HgCl2 and CH3HgCl and incubated at 37ºC for 48 h. Genotoxicity was assessed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylmercury; Cytotoxicity; Genotoxicity; Mitotic index; Human lymphocytes; Chromosome aberrations.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600012

Effect of estrogen receptor-alpha (ESR1) gene polymorphism on high density lipoprotein levels in response to hormone replacement therapy
Provedor de dados: 56
Autores: Nogueira-de-Souza,N.C.; Guerreiro da Silva,I.D.C.; de Carvalho,C.V.; Pulchinelli,A.; Haidar,M.A.; Baracat,E.C.; Massad-Costa,A.M.
Studies have shown that estrogen replacement therapy and estrogen plus progestin replacement therapy alter serum levels of total, LDL and HDL cholesterol levels. However, HDL cholesterol levels in women vary considerably in response to hormone replacement therapy (HRT). A significant portion of the variability of these levels has been attributed to genetic factors. Therefore, we investigated the influence of estrogen receptor-alpha (ESR1) gene polymorphisms on HDL levels in response to postmenopausal HRT. We performed a prospective cohort study on 54 postmenopausal women who had not used HRT before the study and had no significant general medical illness. HRT consisted of conjugated equine estrogen and medroxyprogesterone acetate continuously for 1 year....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hormone replacement therapy; High-density lipoprotein cholesterol levels; Estrogen receptor-alpha; Polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200003

Imidazoline receptors in the heart: a novel target and a novel mechanism of action that involves atrial natriuretic peptides
Provedor de dados: 56
Autores: Mukaddam-Daher,S.; Gutkowska,J.
Chronic stimulation of sympathetic nervous activity contributes to the development and maintenance of hypertension, leading to left ventricular hypertrophy (LVH), arrhythmias and cardiac death. Moxonidine, an imidazoline antihypertensive compound that preferentially activates imidazoline receptors in brainstem rostroventrolateral medulla, suppresses sympathetic activation and reverses LVH. We have identified imidazoline receptors in the heart atria and ventricles, and shown that atrial I1-receptors are up-regulated in spontaneously hypertensive rats (SHR), and ventricular I1-receptors are up-regulated in hamster and human heart failure. Furthermore, cardiac I1-receptor binding decreased after chronic in vivo exposure to moxonidine. These studies implied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sympathetic nerve activity; Imidazoline receptors; Moxonidine; Atrial natriuretic peptide; Brain natriuretic peptide; Hypertension; Heart.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800015

Leptin receptor (Ob-R) mRNA expression and serum leptin concentration in patients with colorectal and metastatic colorectal cancer
Provedor de dados: 56
Autores: Erkasap,N.; Ozkurt,M.; Erkasap,S.; Yasar,F.; Uzuner,K.; Ihtiyar,E.; Uslu,S.; Kara,M.; Bolluk,O.
The objective of the present study was to investigate the effect of leptin on the progression of colorectal carcinoma to metastatic disease by analyzing the serum leptin concentration and Ob-R gene expression in colon cancer tissues. Tissue samples were obtained from 31 patients who underwent surgical resection for colon (18 cases) and metastatic colon (13 cases) cancer. Serum leptin concentration was determined by an enzyme-linked immunosorbent assay (ELISA) and Ob-R mRNA expression by real-time polymerase chain reaction (RT-PCR) for both groups. ELISA data were analyzed by the Student t-test and RT-PCR data were analyzed by the Mann-Whitney U-test. RT-PCR results demonstrated that mRNA expression of Ob-R in human metastatic colorectal cancer was higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptin; Colorectal cancer; Metastatic colorectal cancer; Leptin receptor (Ob-R); Real-time PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300306

Dose-dependent in vitro inhibition of rabbit corneal matrix metalloproteinases by an extract of Pothomorphe umbellata after alkali injury
Provedor de dados: 56
Autores: Barros,L.F.M.; Barros,P.S.M.; Röpke,C.D.; Silva,V.V.; Sawada,T.C.H.; Barros,S.B.M.; Belfort Jr.,R.
The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. The activity was also measured in untreated corneas. After electrophoresis of 20 µg protein, gels were incubated with 50, 100, or 250 µg/mL lyophilized hydroethanolic (1:1) root crude extract of P. umbellata standardized for 4-nerolidylcatechol (7.09%). The activity of the enzymes was compared with that of untreated gel. At 48 h after injury, the activity of all MMPs...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cornea; Alkali injury; Metalloproteinases; Pothomorphe umbellata.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800014

Feasibility of non-intubated anesthesia and regional block for thoracoscopic surgery under spontaneous respiration: a prospective cohort study
Provedor de dados: 56
Autores: Li,Hanwei; Huang,Daiqiang; Qiao,Kun; Wang,Zheng; Xu,Shiyuan.
Data about the feasibility and safety of thoracoscopic surgery under non-intubated anesthesia and regional block are limited. In this prospective study, 57 consecutive patients scheduled for thoracoscopic surgery were enrolled. Patients were sedated with dexmedetomidine and anesthetized with propofol and remifentanil. Ropivacaine was used for intercostal nerve and paravertebral block. Lidocaine was used for vagal block. The primary outcomes were mean arterial pressure (MAP), heart rate (HR), oxygen saturation, and end-tidal carbon dioxide partial pressure (ETCO2) at T0 (pre-anesthesia), T1 (immediately after laryngeal mask/nasopharyngeal airway placement), T2 (immediately after skin incision), T3 (10 min after opening the chest), T4 (end of surgery), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-intubated anesthesia; Paravertebral block; Postoperative recovery; Thoracoscopy; Spontaneous respiration.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100605

Association between tumor necrosis factor polymorphisms and rheumatoid arthritis as well as systemic lupus erythematosus: a meta-analysis
Provedor de dados: 56
Autores: Chen,Lin; Huang,Zhuochun; Liao,Yun; Yang,Bin; Zhang,Junlong.
Tumor necrosis factor-alpha (TNF-α) plays an important role in autoimmune diseases. Previous studies have investigated the association of TNF-α-238G/A (rs361525) and -308G/A (rs1800629) polymorphisms with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). However, no agreed conclusion had been made. Therefore, this meta-analysis was conducted to assess the associations of TNF-α-238G/A and -308G/A polymorphisms with RA and SLE risk. A systematic search was conducted in commonly used databases. Meta-analysis was performed by STATA12.0. A total of 43 studies were included. In the overall population, the TNF-α-238A allele was observed to be a protective factor for RA (A vs G: OR=0.75, 95%CI=0.57–0.99, P=0.040) and the TNF-α-308A allele was found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; Single-nucleotide polymorphism; Systemic lupus erythematosus; Tumor necrosis factor-alpha; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300607

Fat gain with physical detraining is correlated with increased glucose transport and oxidation in periepididymal white adipose tissue in rats
Provedor de dados: 56
Autores: Sertié,R.A.L.; Andreotti,S.; Proença,A.R.G.; Campaña,A.B.; Lima,F.B.
As it is a common observation that obesity tends to occur after discontinuation of exercise, we investigated how white adipocytes isolated from the periepididymal fat of animals with interrupted physical training transport and oxidize glucose, and whether these adaptations support the weight regain seen after 4 weeks of physical detraining. Male Wistar rats (45 days old, weighing 200 g) were divided into two groups (n=10): group D (detrained), trained for 8 weeks and detrained for 4 weeks; and group S (sedentary). The physical exercise was carried out on a treadmill for 60 min/day, 5 days/week for 8 weeks, at 50-60% of the maximum running capacity. After the training protocol, adipocytes isolated from the periepididymal adipose tissue were submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical detraining; Adipocytes; Glucose uptake; Glucose oxidation; Lipogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700650

Further analysis of open-respirometry systems: an a-compartmental mechanistic approach
Provedor de dados: 56
Autores: Chaui-Berlinck,J.G.; Bicudo,J.E.P.W.
A system is said to be "instantaneous" when for a given constant input an equilibrium output is obtained after a while. In the meantime, the output is changing from its initial value towards the equilibrium one. This is the transient period of the system and transients are important features of open-respirometry systems. During transients, one cannot compute the input amplitude directly from the output. The existing models (e.g., first or second order dynamics) cannot account for many of the features observed in real open-respirometry systems, such as time lag. Also, these models do not explain what should be expected when a system is speeded up or slowed down. The purpose of the present study was to develop a mechanistic approach to the dynamics of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Open-respirometry systems; Thermodynamic model.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800014

Ilex paraguariensis extracts extend the lifespan of Drosophila melanogaster fed a high-fat diet
Provedor de dados: 56
Autores: Colpo,A.C.; Lima,M.E.; da Rosa,H.S.; Leal,A.P.; Colares,C.C.; Zago,A.C.; Salgueiro,A.C.F.; Bertelli,P.R.; Minetto,L.; Moura,S.; Mendez,A.S.L.; Folmer,V.
Studies have suggested that total energy intake and diet composition affect lifespan and ageing. A high-fat diet induces oxidative stress and affects the development of diseases. In contrast, antioxidants are capable of reducing its harmful effects. Yerba mate beverages are an important source of antioxidants, but there is scarce knowledge about their effects on suppressing fat accumulation. Here, we investigated the compounds present in yerba mate extracts and assessed their effects on Drosophila melanogaster given a high cholesterol diet. LS-ESI-MS analysis showed the presence of matesaponins, phenolic compounds and methylxanthines in all of the examined extracts. In Drosophila, under extract treatment conditions, the mean lifespan was significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba mate; Matesaponin; LC-MS; Antioxidants compounds; Chronic diseases; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200601

Effect of Camellia sinensis teas on left ventricular hypertrophy and insulin resistance in dyslipidemic mice
Provedor de dados: 56
Autores: Ferreira,M.C.L.; Lima,L.N.; Cota,L.H.T.; Costa,M.B.; Orsi,P.M.E.; Espíndola,R.P.; Albanez,A.V.; Rosa,B.B.; Carvalho,M.G.S.; Garcia,J.A.D.
The control of dyslipidemia using plants is an important subject of studies since it has numerous benefits in cardiovascular protection. The objective of this study was to evaluate the effect of three Camellia sinensis L. teas (green, red, and white) on left ventricular hypertrophy and insulin resistance in low-density lipoprotein receptor knockout (LDLr-/-) mice fed a high-fat diet. The LDLr-/- mice were divided into four experimental groups: Group C: standard feed; Group CT: standard feed and three teas, Group HL: high-fat feed; HLT Group: high-fat feed and three teas. The three types of tea (green, red, and white) originated from different processing of the Camellia sinensis L. plant, and were administered associated once a day at a dose of 25 mg/kg by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipids; Cardiovascular system; Tea; Glucose; Insulin.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500607

RANK, RANKL and osteoprotegerin in arthritic bone loss
Provedor de dados: 56
Autores: Bezerra,M.C.; Carvalho,J.F.; Prokopowitsch,A.S.; Pereira,R.M.R.
Rheumatoid arthritis is characterized by the presence of inflammatory synovitis and destruction of joint cartilage and bone. Tissue proteinases released by synovia, chondrocytes and pannus can cause cartilage destruction and cytokine-activated osteoclasts have been implicated in bone erosions. Rheumatoid arthritis synovial tissues produce a variety of cytokines and growth factors that induce monocyte differentiation to osteoclasts and their proliferation, activation and longer survival in tissues. More recently, a major role in bone erosion has been attributed to the receptor activator of nuclear factor kappa B ligand (RANKL) released by activated lymphocytes and osteoblasts. In fact, osteoclasts are markedly activated after RANKL binding to the cognate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoprotegerin; RANK/RANKL; Arthritis; Bone loss; Erosion.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200004

Time-course effects of aerobic exercise training on cardiovascular and renal parameters in 2K1C renovascular hypertensive rats
Provedor de dados: 56
Autores: Maia,R.C.A.; Sousa,L.E.; Santos,R.A.S.; Silva,M.E.; Lima,W.G.; Campagnole-Santos,M.J.; Alzamora,A.C.
Exercise training (Ex) has been recommended for its beneficial effects in hypertensive states. The present study evaluated the time-course effects of Ex without workload on mean arterial pressure (MAP), reflex bradycardia, cardiac and renal histology, and oxidative stress in two-kidney, one-clip (2K1C) hypertensive rats. Male Fischer rats (10 weeks old; 150–180 g) underwent surgery (2K1C or SHAM) and were subsequently divided into a sedentary (SED) group and Ex group (swimming 1 h/day, 5 days/week for 2, 4, 6, 8, or 10 weeks). Until week 4, Ex decreased MAP, increased reflex bradycardia, prevented concentric hypertrophy, reduced collagen deposition in the myocardium and kidneys, decreased the level of thiobarbituric acid-reactive substances (TBARS) in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2K1C renovascular hypertension; Swimming; Baroreflex bradycardia; Heart and kidney adaptations; Oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101010

Perspectives of digestive pest control with proteinase inhibitors that mainly affect the trypsin-like activity of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae)
Provedor de dados: 56
Autores: Pereira,M.E.; Dörr,F.A.; Peixoto,N.C.; Lima-Garcia,J.F.; Dörr,F.; Brito,G.G.
The present study describes the main characteristics of the proteolytic activities of the velvetbean caterpillar, Anticarsia gemmatalis Hübner, and their sensitivity to proteinase inhibitors and activators. Midguts of last instar larvae reared on an artificial diet were homogenized in 0.15 M NaCl and centrifuged at 14,000 g for 10 min at 4ºC and the supernatants were used in enzymatic assays at 30ºC, pH 10.0. Basal total proteolytic activity (azocasein hydrolysis) was 1.14 ± 0.15 absorbance variation min-1 mg protein-1, at 420 nm; basal trypsin-like activity (N-benzoyl-L-arginine-p-nitroanilide, BApNA, hydrolysis) was 0.217 ± 0.02 mmol p-nitroaniline min-1 mg protein-1. The maximum proteolytic activities were observed at pH 10.5 using azocasein and at pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticarsia gemmatalis (Lepidoptera: Noctuidae); Azocasein hydrolysis; BApNA hydrolysis; Proteinase inhibitors; Protein digestion; Serine-proteinases.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100010

Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum
Provedor de dados: 56
Autores: Batista,S.S.; Pires,R.S.; Britto,L.R.G.
Glutamate receptors have been often associated with developmental processes. We used immunohistochemical techniques to evaluate the expression of the AMPA-type glutamate receptor (GluR) subunits in the chick optic tectum (TeO). Chick embryos from the 5th through the 20th embryonic day (E5-E20) and one-day-old (P1) chicks were used. The three types of immunoreactivity evaluated (GluR1, GluR2/3, and GluR4) had different temporal and spatial expression patterns in the several layers of the TeO. The GluR1 subunit first appeared as moderate staining on E7 and then increased on E9. The mature GluR1 pattern included intense staining only in layer 5 of the TeO. The GluR2/3 subunits presented low expression on E5, which became intense on E7. The staining for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: AMPA; Development; Glutamate receptors; Neurotransmitters; Receptor subunits; Visual system.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800015

Does acute hyperglycemia alter rat aortic depressor nerve function?
Provedor de dados: 56
Autores: Huber,D.A.; Carmo,J.M. do; Castania,J.A.; Fazan Jr,R.; Salgado,H.C.
Because it is not known where in the reflex arch, i.e., afference, central nervous system or efferences, hyperglycemia affects baroreflex function, the present study examined the effect of short-term (30 min) hyperglycemia on aortic depressor nerve function measured by a mean arterial pressure vs aortic depressor nerve activity curve, fitted by sigmoidal regression, or by cross-spectral analysis between mean arterial pressure and aortic depressor nerve activity. Anesthetized male Wistar rats received an intravenous bolus (0.25 mL) injection, followed by 30 min of infusion (1 mL/h) of 30% glucose (N = 14). Control groups received a bolus injection and infusion of 0.9% saline (N = 14), or 30% mannitol (N = 14). Glucose significantly increased both blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pressoreceptors; Hyperglycemia; Arterial pressure; Spectral analysis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100017

Effect of pyloroplasty and fundectomy on the delay of gastric emptying and gastrointestinal transit of liquid elicited by acute blood volume expansion in awake rats
Provedor de dados: 56
Autores: Rêgo,M.C.V.; da-Graça,J.R.V.; de-A.A. Gondim,F.; de-M. Gondim,R.B.; Dantas,R.P.; Rola,F.H.
We evaluated the effects of fundectomy and pyloroplasty on the delay of gastric emptying (GE) and gastrointestinal (GI) transit of liquid due to blood volume (BV) expansion in awake rats. Male Wistar rats (N = 76, 180-250 g) were first submitted to fundectomy (N = 26), Heinecke-Mikulicz pyloroplasty (N = 25) or SHAM laparotomy (N = 25). After 6 days, the left external jugular vein was cannulated and the animals were fasted for 24 h with water ad libitum. The test meal was administered intragastrically (1.5 ml of a phenol red solution, 0.5 mg/ml in 5% glucose) to normovolemic control animals and to animals submitted to BV expansion (Ringer-bicarbonate, iv infusion, 1 ml/min, volume up to 5% body weight). BV expansion decreased GE and GI transit rates in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Gastrointestinal transit; Blood volume expansion; Fundectomy; Pyloroplasty; Vagotomy.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300016

Cryptic mosaicism involving a second chromosome X in patients with Turner syndrome
Provedor de dados: 56
Autores: Araújo,A.; Ramos,E.S.
The high abortion rate of 45,X embryos indicates that patients with Turner syndrome and 45,X karyotype could be mosaics, in at least one phase of embryo development or cellular lineage, due to the need for the other sex chromosome presence for conceptus to be compatible with life. In cases of structural chromosomal aberrations or hidden mosaicism, conventional cytogenetic techniques can be ineffective and molecular investigation is indicated. Two hundred and fifty patients with Turner syndrome stigmata were studied and 36 who had female genitalia and had been cytogenetically diagnosed as having "pure" 45,X karyotype were selected after 100 metaphases were analyzed in order to exclude mosaicism and the presence of genomic Y-specific sequences (SRY, TSPY,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turner syndrome; X-inactivation; HUMARA assay; DNA methylation; Criptic mosaicism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500004

Presurgical ketoprofen, but not morphine, dipyrone, diclofenac or tenoxicam, preempts post-incisional mechanical allodynia in rats
Provedor de dados: 56
Autores: Prado,W.A.; Pontes,R.M.C.
The treatment of pain before it initiates may prevent the persistent pain-induced changes in the central nervous system that amplify pain long after the initial stimulus. The effects of pre- or postoperative intraperitoneal administration of morphine (2 to 8 mg/kg), dipyrone (40 and 80 mg/kg), diclofenac (2 to 8 mg/kg), ketoprofen (10 and 20 mg/kg), and tenoxicam (10 and 20 mg/kg) were studied in a rat model of post-incisional pain. Groups of 5 to 8 male Wistar rats (140-160 g) were used to test each drug dose. An incision was made on the plantar surface of a hind paw and the changes in the withdrawal threshold to mechanical stimulation were evaluated with Von Frey filaments at 1, 2, 6 and 24 h after the surgery. Tenoxicam was given 12 or 6 h...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preemptive analgesia; Morphine; Dipyrone; Diclofenac; Ketoprofen; Tenoxicam.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100016

Association study of AFF1 rs340630 polymorphism with genetic susceptibility to rheumatoid arthritis in Chinese population
Provedor de dados: 56
Autores: Sun,Qing-Qing; Hua,Dong-Jin; Huang,Si-Chao; Cen,Han; Zhou,Li; Shao,Song.
This study was performed to examine whether the AF4/FMR2 family, member 1 (AFF1) rs340630 polymorphism is involved in the genetic background of rheumatoid arthritis (RA) in a Chinese population. Two different study groups of RA patients and controls (328 RA patients and 449 healthy controls in the first study group; 232 RA patients and 313 controls in the second study group) were included in our study. Overall, there was no significant difference in either genotype (P=0.71 and 0.64 in the first and second study group, respectively) nor allele (in the first study group: A vs G, P=0.65, OR=1.05, 95%CI=0.85–1.29; in the second study group: G vs A, P=0.47, OR=1.10, 95%CI=0.86–1.40) frequencies of AFF1 rs340630 polymorphism between RA patients and controls. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; AFF1; Polymorphism; Chinese.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700606

Critical role of metabotropic glutamate receptor 4 in bone marrow-derived dendritic cells in the Th17 cell differentiation and the melanogenesis of B16 cells
Provedor de dados: 56
Autores: Zhao,Guangming; Zhou,Wenhui; Liu,Ying; Wang,Yupeng; Li,Zhou; Song,Zhiqi.
Vitiligo is an acquired pigmentary disorder resulting from selective destruction of melanocytes. Emerging studies have suggested that T helper cell 17 (Th17) is potentially implicated in vitiligo development and progression. It was recently discovered that metabotropic glutamate receptor 4 (mGluR4) can modulate Th17-mediated adaptive immunity. However, the influence of mGluR4 on melanogenesis of melanocytes has yet to be elucidated. In the present study, we primarily cultured mouse bone marrow-derived dendritic cells (BMDC) and then knocked down and over-expressed mGluR4 using transfection. Transduced BMDC were co-cultured with CD4+ T cells and the expression of Th17-related cytokines were measured. The morphology and melanogenesis of B16 cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabotropic glutamate receptor-4; Th17; Melanogenesis; Vitiligo.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400604

Effects of submaximal exercise with water ingestion on intraocular pressure in healthy human males
Provedor de dados: 56
Autores: Moura,M.A.; Rodrigues,L.O.C.; Waisberg,Y.; de Almeida,H.G.; Silami-Garcia,E.
The effects of exercise and water replacement on intraocular pressure (IOP) have not been well established. Furthermore, it is not known whether the temperature of the fluid ingested influences the IOP response. In the present study we determined the effect of water ingestion at three temperatures (10, 24 and 38ºC; 600 ml 15 min before and 240 ml 15, 30 and 45 min after the beginning of each experimental session) on the IOP of six healthy male volunteers (age = 24.0 ± 3.5 years, weight = 67.0 ± 4.8 kg, peak oxygen uptake (VO2peak) = 47.8 ± 9.1 ml kg-1 min-1). The subjects exercised until exhaustion on a cycle ergometer at a 60% VO2peak in a thermoneutral environment. IOP was measured before and after exercise and during recovery (15, 30 and 45 min) using...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Exercise; Intraocular pressure; Thermal regulation; Water ingestion.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100017

Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats
Provedor de dados: 56
Autores: Oliveira,R.B.; Gomes-Leal,W.; do-Nascimento,J.L.M.; Picanço-Diniz,C.W.
The effects of methylmercury (MeHg) on histochemical demonstration of the NADPH-diaphorase (NADPH-d) activity in the striate cortex were studied in 4 adult cats. Two animals were used as control. The contaminated animals received 50 ml milk containing 0.42 µg MeHg and 100 g fish containing 0.03 µg MeHg daily for 2 months. The level of MeHg in area 17 of intoxicated animals was 3.2 µg/g wet weight brain tissue. Two cats were perfused 24 h after the last dose (group 1) and the other animals were perfused 6 months later (group 2). After microtomy, sections were processed for NADPHd histochemistry procedures using the malic enzyme method. Dendritic branch counts were performed from camera lucida drawings for control and intoxicated animals (N = 80). Average,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: NADPH-dehydrogenase; Visual cortex; Neurotoxicity; Neuropil; Mercury.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900009

Basic life support knowledge of first-year university students from Brazil
Provedor de dados: 56
Autores: Santos,S. V.; Margarido,M. R. R. A.; Caires,I. S.; Santos,R. A. N.; Souza,S. G.; Souza,J. M. A.; Martimiano,R. R.; Dutra,C. S. K.; Palha,P.; Zanetti,A. C. G.; Pazin-Filho,A.
We aimed to evaluate knowledge of first aid among new undergraduates and whether it is affected by their chosen course. A questionnaire was developed to assess knowledge of how to activate the Mobile Emergency Attendance Service - MEAS (Serviço de Atendimento Móvel de Urgência; SAMU), recognize a pre-hospital emergency situation and the first aid required for cardiac arrest. The students were also asked about enrolling in a first aid course. Responses were received from 1038 of 1365 (76.04%) new undergraduates. The questionnaires were completed in a 2-week period 1 month after the beginning of classes. Of the 1038 respondents (59.5% studying biological sciences, 11.6% physical sciences, and 28.6% humanities), 58.5% knew how to activate the MEAS/SAMU (54.3%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basic life support; Undergraduate education; Cardiac arrest; Pre-hospital; First Aid.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201151

Changes in types of muscle fibers induced by transcutaneous electrical stimulation of the diaphragm of rats
Provedor de dados: 56
Autores: Costa,D.; Cancelliero,K.M.; Campos,G.E.R.; Salvini,T.F.; da Silva,C.A.
The objective of the present study was to assess the effect of transcutaneous electrical diaphragmatic stimulation (TEDS) on different types of diaphragm muscle fibers. Male Wistar rats (8-12 weeks old) were divided into 2 experimental groups (N = 8 in each group): 1) control, 2) animals submitted to TEDS [frequency = 50 Hz; T ON/T OFF (contraction/relaxation time) = 2/2 s; pulse duration = 0.4 ms, intensity = 5 mA with a 1 mA increase every 3 min for 20 min] for 7 days. After completing this treatment period, the I, IIA, IIB, and IID diaphragm muscle fibers were identified using the mATPase technique. Statistical analysis consisted of the normality, homoscedasticity and t-tests (P &lt; 0.05). There was a 19.6% (P &lt; 0.05) reduction in the number of type...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diaphragm; Transcutaneous electrical stimulation; Muscle fiber.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900011

High concentrations of KCl release noradrenaline from noradrenergic neurons in the rat anococcygeus muscle
Provedor de dados: 56
Autores: Araujo,C.B.L.; Bendhack,L.M.
The aim of the present study was to investigate the effects of high concentrations of KCl in releasing noradrenaline from sympathetic nerves and its actions on postsynaptic alpha-adrenoceptors. We measured the isotonic contractions induced by KCl in the isolated rat anococcygeus muscle under different experimental conditions. The contractile responses induced by KCl were inhibited by alpha-adrenoceptor antagonists in 2.5 mM Ca2+ solution. Prazosin reduced the maximum effect from 100 to 53.9 ± 10.2% (P&lt;0.05) while the pD2 values were not changed. The contractile responses induced by KCl were abolished by prazosin in Ca2+-free solution (P&lt;0.05). Treatment of the rats with reserpine reduced the maximum effect induced by KCl as compared to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Noradrenaline; KCl; Anococcygeus muscle; Alpha-Adrenoceptors; Noradrenergic neurons.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100013

Long non-coding RNA HOTAIR promotes UVB-induced apoptosis and inflammatory injury by up-regulation of PKR in keratinocytes
Provedor de dados: 56
Autores: Liu,Guo; Zhang,Wenhao.
Excessive exposure to ultraviolet (UV) rays can cause damage of the skin and may induce cancer, immunosuppression, photoaging, and inflammation. The long non-coding RNA (lncRNA) HOX antisense intergenic RNA (HOTAIR) is involved in multiple human biological processes. However, its role in UVB-induced keratinocyte injury is unclear. This study was performed to investigate the effects of HOTAIR in UVB-induced apoptosis and inflammatory injury in human keratinocytes (HaCaT cells). Quantitative real-time polymerase chain reaction was performed to analyze the expression levels of HOTAIR, PKR, TNF-α, and IL-6. Cell viability was measured using trypan blue exclusion method and cell apoptosis using flow cytometry and western blot. ELISA was used to measure the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HOTAIR; UVB-induced apoptosis; Inflammatory injury; PKR; Keratinocytes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800607

Estrogen and progesterone receptors in human papilloma virus-related cervical neoplasia
Provedor de dados: 56
Autores: Coelho,F.R.G.; Prado,J.C.M.; Pereira Sobrinho,J.S.; Hamada,G.; Landman,G.; Pinto,C.A.; Nonogaki,S.; Villa,L.L.
Estrogen (ER) and progesterone (PR) receptors in the normal uterine cervix, cervical intraepithelial neoplasia and invasive carcinoma were studied in consecutive samples from Hospital do Câncer, São Paulo, between 1996 and 1997. Tissue was collected by removing a fragment of the tumoral area using a 5-mm diameter biopsy punch, followed by removal of a macroscopically normal area as close as possible from the tumor. Histopathological confirmation was obtained for all specimens analyzed. A total of 24 normal tissues, 17 cases of cervical intraepithelial neoplasia and 7 of invasive carcinomas were studied. The ER/PR ratio was determined by immunohistochemistry using monoclonal antibodies specific for each receptor. Adjacent tissue slides were submitted to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estrogen receptor; Progesterone receptor; Cervical cancer; Cervical intraepithelial neoplasia; Human papillomavirus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100012

In vitro activity of hypnophilin from Lentinus strigosus: a potential prototype for Chagas disease and leishmaniasis chemotherapy
Provedor de dados: 56
Autores: Souza-Fagundes,E.M.; Cota,B.B.; Rosa,L.H.; Romanha,A.J.; Corrêa-Oliveira,R.; Rosa,C.A.; Zani,C.L.; Teixeira-Carvalho,A.; Martins-Filho,O.A.
Hypnophilin and panepoxydone, terpenoids isolated from Lentinus strigosus, have significant inhibitory activity onTrypanosoma cruzi trypanothione reductase (TR). Although they have similar TR inhibitory activity at 10 μg/mL (40.3 μM and 47.6 μM for hypnophilin and panepoxydone, respectively; ~100%), hypnophilin has a slightly greater inhibitory activity (~71%) on T. cruzi amastigote (AMA) growth in vitro as well as on in vitro phytohemagglutinin (PHA)-induced peripheral blood mononuclear (PBMC) proliferation (~70%) compared to panepoxydone (69% AMA inhibition and 91% PBMC inhibition). Hypnophilin and panepoxydone at 1.25 μg/mL had 67% inhibitory activity onLeishmania (Leishmania) amazonensis amastigote-like (AMA-like) growth in vitro. The panepoxydone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas disease; Leishmaniasis; Chemotherapy; Immunomodulation; Hypnophilin; Lentinus strigosus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100007

Protective effect of bronchial challenge with hypertonic saline on nocturnal asthma
Provedor de dados: 56
Autores: Borges,M.C.; Ferraz,E.; Terra-Filho,J.; Vianna,E.O.
Inhalation of hypertonic saline (HS) causes bronchoconstriction in asthmatic subjects. Repeated inhalation of HS leads to substantially reduced bronchoconstriction, known as the refractory period. Refractoriness due to different stimuli has also been described (cross-refractoriness). Nocturnal asthma is defined as an increase in symptoms, need for medication, airway responsiveness, and/or worsening of lung function that usually occurs from 4 to 6 am. Our objective was to determine the effect of refractoriness on nocturnal asthma. The challenge test consisted of inhalations of 4.5% saline with increasing durations until a reduction of 20% in forced expiratory volume in 1 s (FEV1) (PD20HS) or total time of 15.5 min. Twelve subjects with nocturnal asthma were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Refractory period; Bronchial challenge; Hypertonic saline; Bronchial provocation test.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300006

Lack of relationship between glycemic control and bone mineral density in type 2 diabetes mellitus
Provedor de dados: 56
Autores: Cutrim,D.M.S.L.; Pereira,F.A.; Paula,F.J.A. de; Foss,M.C.
We assessed the effect of chronic hyperglycemia on bone mineral density (BMD) and bone remodeling in patients with type 2 diabetes mellitus. We investigated 42 patients with type 2 diabetes under stable control for at least 1 year, 22 of them with good metabolic control (GMC: mean age = 48.8 ± 1.5 years, 11 females) and 20 with poor metabolic control (PMC: mean age = 50.2 ± 1.2 years, 8 females), and 24 normal control individuals (CG: mean age = 46.5 ± 1.1 years, 14 females). We determined BMD in the femoral neck and at the L2-L4 level (DEXA) and serum levels of glucose, total glycated hemoglobin (HbA1), total and ionic calcium, phosphorus, alkaline phosphatase, follicle-stimulating hormone, intact parathyroid hormone (iPTH), 25-hydroxyvitamin D (25-OH-D),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Osteoporosis; Bone mineral density; Glycemic control; Bone markers; Insulin-like growth factor I.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200008

Inadequate utilization of prenatal care in two Brazilian birth cohorts
Provedor de dados: 56
Autores: Coimbra,L.C.; Figueiredo,F.P.; Silva,A.A.M.; Barbieri,M.A.; Bettiol,H.; Caldas,A.J.M.; Mochel,E.G.; Ribeiro,V.S.
Data for two birth cohorts from two Brazilian municipalities, Ribeirão Preto in 1994 and São Luís in 1997/1998, were used to identify and compare factors associated with inadequate utilization of prenatal care and to identify factors capable of explaining the differences observed between the two cities. Prenatal care was defined as adequate or inadequate according to the recommendations of the Brazilian Ministry of Health. The chi-square test and Poisson regression were used to compare differences in the inadequacy of prenatal care utilization. The percentage of inadequacy was higher in São Luís (34.6%) than in Ribeirão Preto (16.9%). Practically the same variables were associated with inadequacy in both cities. Puerperae with lower educational level,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prenatal care; Maternal health; Quality of health services.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900006

Neonatal tobacco smoke reduces thermogenesis capacity in brown adipose tissue in adult rats
Provedor de dados: 56
Autores: Peixoto,T.C.; Moura,E.G.; Oliveira,E.; Younes-Rapozo,V.; Soares,P.N.; Rodrigues,V.S.T.; Santos,T.R.; Peixoto-Silva,N.; Carvalho,J.C.; Calvino,C.; Conceição,E.P.S.; Guarda,D.S.; Claudio-Neto,S.; Manhães,A.C.; Lisboa,P.C.
Maternal smoking is a risk factor for progeny obesity. We have previously shown, in a rat model of neonatal tobacco smoke exposure, a mild increase in food intake and a considerable increase in visceral adiposity in the adult offspring. Males also had secondary hyperthyroidism, while females had only higher T4. Since brown adipose tissue (BAT) hypofunction is related to obesity, here we tested the hypothesis that higher levels of thyroid hormones are not functional in BAT, suggesting a lower metabolic rate. We evaluated autonomic nerve activity in BAT and its function in adult rats that were exposed to tobacco smoke during lactation. At birth, litters were adjusted to 3 male and 3 female pups/litter. From postnatal day (PND) 3 to 21, Wistar lactating rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tobacco smoke Suckling period Developmental plasticity; Obesity Autonomic function.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600602

Levels of C-reactive protein in serum samples from healthy children and adults in São Paulo, Brazil
Provedor de dados: 56
Autores: Ribeiro,M.A.
C-reactive protein (CRP) was measured by ELISA in the sera of 165 healthy blood donors and 125 normal children 1 to 14 years old. The serum levels of blood donors ranged from 0.05 to 57.6 mg/l with median and mean values of 1.8 mg/l and 4.86 mg/l, respectively. CRP levels ranged from 0.02 to 14.4 mg/l in the children's sera, the median being 0.45 mg/l and the mean 1.65 mg/l. No individual lacking CRP was detected. The high CRP levels observed in the present study suggest that the population of the State of São Paulo may usually be exposed to subacute infections and/or inflammation without presenting clinical symptoms
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-reactive protein (CRP); Serum CRP.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900002

MicroRNA-455 suppresses the oncogenic function of HDAC2 in human colorectal cancer
Provedor de dados: 56
Autores: Mao,Q.D.; Zhang,W.; Zhao,K.; Cao,B.; Yuan,H.; Wei,L.Z.; Song,M.Q.; Liu,X.S.
Colorectal cancer (CRC) is the fourth leading cause of cancer-induced mortality. Histone deacetylase 2 (HDAC2) is involved in prognosis and therapy of CRC. This study aimed to explore novel therapeutic targets for CRC. The alteration of HDAC2 expression in CRC tissues was estimated by qRT-PCR. After lentivirus transfection, HDAC2 knockdown was confirmed by western blot analysis. The effect of HDAC2 knockdown on cell proliferation was then assessed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Screened by TargetScan, microRNA (miR)-455 was predicted to bind to 3′UTR of HDAC2 and the prediction was verified by luciferase assay. Finally, cells were transfected, respectively, with miR-455 mimics or miR-455 negative control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal cancer; Histone deacetylase 2; MiR-455; Proliferation; Apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600608

Nonspecific blockade of vascular free radical signals by methylated arginine analogues
Provedor de dados: 56
Autores: Pedro,M.A.; Augusto,O.; Barbeiro,H.V.; Carvalho,M.H.C.; da-Luz,P.L.; Laurindo,F.R.M.
Methylated arginine analogues are often used as probes of the effect of nitric oxide; however, their specificity is unclear and seems to be frequently overestimated. This study analyzed the effects of NG-methyl-L-arginine (L-NMMA) on the endothelium-dependent release of vascular superoxide radicals triggered by increased flow. Plasma ascorbyl radical signals measured by direct electron paramagnetic resonance spectroscopy in 25 rabbits increased by 3.8 ± 0.7 nmol/l vs baseline (28.7 ± 1.4 nmol/l, P&amp;lt;0.001) in response to papaverine-induced flow increases of 121 ± 12%. In contrast, after similar papaverine-induced flow increases simultaneously with L-NMMA infusions, ascorbyl levels were not significantly changed compared to baseline. Similar results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Free radicals; Arginine analogues; NG-methyl-L-arginine; NG-methyl-D-arginine; NG-nitro-L-arginine methyl ester.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600004

Immune strategies using single-component LipL32 and multi-component recombinant LipL32-41-OmpL1 vaccines against leptospira
Provedor de dados: 56
Autores: Feng,C.Y.; Li,Q.T.; Zhang,X.Y.; Dong,K.; Hu,B.Y.; Guo,X.K.
Genes encoding lipoproteins LipL32, LipL41 and the outer-membrane protein OmpL1 of leptospira were recombined and cloned into a pVAX1 plasmid. BALB/c mice were immunized with LipL32 and recombined LipL32-41-OmpL1 using DNA-DNA, DNA-protein and protein-protein strategies, respectively. Prime immunization was on day 1, boost immunizations were on day 11 and day 21. Sera were collected from each mouse on day 35 for antibody, cytokine detection and microscopic agglutination test while spleen cells were collected for splenocyte proliferation assay. All experimental groups (N = 10 mice per group) showed statistically significant increases in antigen-specific antibodies, in cytokines IL-4 and IL-10, as well as in the microscopic agglutination test and splenocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune strategy; Recombinant vaccine; Leptospira; Lipoproteins LipL32; LipL41.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900005

Expression of p53 protein in pituitary adenomas
Provedor de dados: 56
Autores: Oliveira,M.C.; Marroni,C.P.; Pizarro,C.B.; Pereira-Lima,J.F.; Barbosa-Coutinho,L.M.; Ferreira,N.P.
Inactivating mutations of TP53, a tumor suppressor gene, are associated with abnormal cell proliferation. Although p53 expression is common in many human malignancies, p53 protein has seldom been evaluated in pituitary tumors. When detected, the percentage of p53-positive cells is low, and, in general, it is exclusive for invasive lesions. The aim of the present study was to use immunohistochemistry to determine the presence of p53 protein in pituitary adenomas from tumor samples of 163 surgeries performed in 148 patients (40% male, 60% female). In 35% of the cases the adenoma was nonfunctional, while in the others it was associated with PRL, GH and/or ACTH endocrine hypersecretion syndrome. Macroadenomas were observed in 83.2% of the cases with available...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pituitary; Pituitary adenoma; Invasive adenoma; P53; Immunohistochemistry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500008

Correlations of circulating peptide YY and ghrelin with body weight, rate of weight gain, and time required to achieve the recommended daily intake in preterm infants
Provedor de dados: 56
Autores: Chen,XiaFang; Du,Xueliang; Zhu,JianXing; Xie,LiJuan; Zhang,YongJun; He,ZhenJuan.
The objective was to elucidate the relationships between serum concentrations of the gut hormone peptide YY (PYY) and ghrelin and growth development in infants for potential application to the clinical observation index. Serum concentrations of PYY and ghrelin were measured using radioimmunoassay from samples collected at the clinic. For each patient, gestational age, birth weight, time required to return to birth weight, rate of weight gain, time required to achieve recommended daily intake (RDI) standards, time required for full-gastric feeding, duration of hospitalization, and time of administration of total parenteral nutrition were recorded. Serum PYY and ghrelin concentrations were significantly higher in the preterm group (N = 20) than in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptide YY; Ghrelin; Preterm infants; Recommended daily intake.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700014

C-reactive protein in acute coronary syndrome: association with 3-year outcomes
Provedor de dados: 56
Autores: Schaan,B.D.; Pellanda,L.C.; Maciel,P.T.; Duarte,E.R.; Portal,V.L.
Inflammatory markers have been associated with clinical outcome in patients with acute coronary syndrome (ACS). The present study evaluated the role of high-sensitivity C-reactive protein (CRP) measurements as a predictor of late cardiovascular outcomes after ACS. One hundred and ninety-nine ACS patients in a Coronary Care Unit from March to November 2002 were included and were reassessed clinically after ~3 years. Clinical variables and CRP levels were evaluated as predictors of major cardiovascular events (MACE, defined as the occurrence of cardiac death, ischemic stroke or myocardial infarction) and mortality. Statistical analyses included Cox multivariable analysis and survival curves (Kaplan-Meier). Of the 199 patients, 11 died within 1 month (5.5%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute coronary syndromes; Inflammatory markers; C-reactive protein; Major cardiovascular events; Mortality.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200019

Chlorpheniramine facilitates inhibitory avoidance in teleosts submitted to telencephalic ablation
Provedor de dados: 56
Autores: Faganello,F.R.; Mattioli,R.
The present study investigated the involvement of H(1) histaminegic receptor on the acquisition of inhibitory avoidance in Carassius auratus submitted to telencephalic ablation. The fish were submitted to telencephalic ablation 5 days before the experiment. The inhibitory avoidance procedure included 1 day for habituation, 3 days for training composed of 3 trials each (1st day: T1, T2, T3; 2nd day: 2T1, 2T2, 2T3; 3rd day: 3T1, 3T2, 3T3) and 1 day for test. On training days, the fish were placed in a white compartment, after 30 s the door was opened. When the fish crossed to a black compartment, a weight was dropped (aversive stimuli). Immediately after the third trial, on training days, the fish received, intraperitoneally, one of the pharmacological...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chlorpheniramine; Inhibitory avoidance; Telencephalic ablation; Teleost.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500008

The secondary alcohol and aglycone metabolites of doxorubicin alter metabolism of human erythrocytes
Provedor de dados: 56
Autores: Misiti,F.; Giardina,B.; Mordente,A.; Clementi,M.E.
Anthracyclines, a class of antitumor drugs widely used for the treatment of solid and hematological malignancies, cause a cumulative dose-dependent cardiac toxicity whose biochemical basis is unclear. Recent studies of the role of the metabolites of anthracyclines, i.e., the alcohol metabolite doxorubicinol and aglycone metabolites, have suggested new hypotheses about the mechanisms of anthracycline cardiotoxicity. In the present study, human red blood cells were used as a cell model. Exposure (1 h at 37ºC) of intact human red blood cells to doxorubicinol (40 µM) and to aglycone derivatives of doxorubicin (40 µM) induced, compared with untreated red cells: i) a ~2-fold stimulation of the pentose phosphate pathway (PPP) and ii) a marked inhibition of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthracyclines; Doxorubicinol; Doxorubicin; Aglycone metabolite; Pentose phosphate pathway; [13C]-NMR spectroscopy; Hemoglobin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200005

Association between the c.910A&amp;gt;G genetic variant of the XRCC1 gene and susceptibility to esophageal cancer in the Chinese Han population
Provedor de dados: 56
Autores: Chen,X.Q.; Wang,F.; Zheng,Y.L.; Fan,Q.X.; Yue,D.L.; Ma,Z.J.
Esophageal cancer (EC) is a common malignancy worldwide. The X-ray repair cross-complementing 1 gene (XRCC1) is one of the most important candidate genes for influencing susceptibility to EC. This study aimed to investigate the effect of XRCC1 genetic variants on susceptibility to EC. A total of 383 EC patients (males: 239, females: 144, mean age: 56.62) and 387 cancer-free controls (males: 251, females: 136, mean age: 58.23) were enrolled in this study. The c.910A&gt;G genetic variant of theXRCC1 gene was determined by polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing methods. The allele and genotype frequencies indicated statistical differences between EC patients and cancer-free controls. The c.910A&gt;G genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal cancer; XRCC1 gene; Genetic variant; Molecular marker; Susceptibility.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201028

Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells
Provedor de dados: 56
Autores: Lauand,C.; Niero,E.L.; Dias,V.M.; Machado-Santelli,G.M.
Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR). ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-small cell lung cancer) and to analyze the possible association between the micronuclei (MNs) and the extrusion of ErbB1 gene extra-copies. After double blocking, by the process of fetal bovine serum deprivation and vincristine treatment, MNs formation was monitored with 5-bromo-2-deoxyuridine (BrdU) incorporation, which is an S-phase marker. Statistical analyses allowed us to infer that MNs may arise both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A549 cells; Cell cycle synchronization; BrdU incorporation; ErbB1; Micronucleus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500382

Dimensions and dynamics in integrin function
Provedor de dados: 56
Autores: Yamada,K.M.; Pankov,R.; Cukierman,E.
Integrins play crucial roles in cell adhesion, migration, and signaling by providing transmembrane links between the extracellular matrix and the cytoskeleton. Integrins cluster in macromolecular complexes to generate cell-matrix adhesions such as focal adhesions. In this mini-review, we compare certain integrin-based biological responses and signaling during cell interactions with standard 2D cell culture versus 3D matrices. Besides responding to the composition of the matrix, cells sense and react to physical properties that include three-dimensionality and rigidity. In routine cell culture, fibroblasts and mesenchymal cells appear to use focal adhesions as anchors. They then use intracellular actomyosin contractility and dynamic, directional integrin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Cell adhesion; Integrin; Fibronectin; Signal transduction; Migration.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800001

Relationship between uric acid levels and risk of chronic kidney disease in a retrospective cohort of Brazilian workers
Provedor de dados: 56
Autores: Chini,L.S.N.; Assis,L.I.S.; Lugon,J.R.
Uric acid (UA) levels are increased in patients with kidney dysfunction. We analyzed the association between asymptomatic hyperuricemia and new-onset chronic kidney disease (CKD). A retrospective cohort study was designed to collect data from employees of an energy generation and distribution company in the city of Rio de Janeiro, Brazil, who had undergone the company’s annual medical checkup from 2008 to 2014. People with ≤2 years of follow-up, with baseline estimated glomerular filtration rate (eGFR) &lt;60 mL·min-1·(1.73 m2)-1 or with incomplete data were excluded. The endpoint was defined as eGFR &lt;60 mL·min-1·(1.73 m2)-1 estimated through the chronic kidney disease epidemiology collaboration equation (CKD-EPI). The study included 1094 participants....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney disease; Hyperuricemia; Glomerular filtration rate.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900611

Thyrotropin and free thyroxine levels and coronary artery disease: cross-sectional analysis of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Provedor de dados: 56
Autores: de Miranda,E.J.F. Peixoto; Bittencourt,M.S.; Staniak,H.L.; Sharovsky,R.; Pereira,A.C.; Foppa,M.; Santos,I.S.; Lotufo,P.A.; Benseñor,I.M.
Data on the association between subclinical thyroid dysfunction and coronary artery disease (CAD) is scarce. We aimed to analyze the association between thyroid function and CAD using baseline data from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). We included subjects with normal thyroid function (0.4-4.0 mIU/L, and normal free thyroxine, FT4, or 0.8 to 1.9 ng/dL), subclinical hypothyroidism (SCHypo; TSH&gt;4.0 mIU/L and normal FT4), and subclinical hyperthyroidism (SCHyper; TSH&lt;0.4 mIU/L and normal FT4) evaluated by coronary computed tomography angiography. We excluded individuals using medications that interfere in thyroid function or with past medical history of cardiovascular disease. Logistic regression models evaluated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid disorders; Thyrotropin levels; Coronary artery disease; Coronary computed tomography angiography; Subclinical atherosclerosis; Cardiovascular disease.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500609

BMP-2 and titanium particles synergistically activate osteoclast formation
Provedor de dados: 56
Autores: Sun,S.X.; Guo,H.H.; Zhang,J.; Yu,B.; Sun,K.N.; Jin,Q.H.
A previous study showed that BMP-2 (bone morphogenetic protein-2) and wear debris can separately support osteoclast formation induced by the receptor activator of NF-κB ligand (RANKL). However, the effect of BMP-2 on wear debris-induced osteoclast formation is unclear. In this study, we show that neither titanium particles nor BMP-2 can induce osteoclast formation in RAW 264.7 mouse leukemic monocyte macrophage cells but that BMP-2 synergizes with titanium particles to enhance osteoclast formation in the presence of RANKL, and that at a low concentration, BMP-2 has an optimal effect to stimulate the size and number of multinuclear osteoclasts, expression of osteoclast genes, and resorption area. Our data also clarify that the effects caused by the increase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone morphogenetic protein; Titanium; Osteoclasts; SMAD; RANKL.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600461

IL-6 upregulation contributes to the reduction of miR-26a expression in hepatocellular carcinoma cells
Provedor de dados: 56
Autores: Zhang,Yafei; Zhang,Bicheng; Zhang,Anran; Li,Xiaohua; Liu,Jian; Zhao,Jie; Zhao,Yong; Gao,Jianfei; Fang,Dianchun; Rao,Zhiguo.
A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-26a; IL-6; C-Myc; Hepatocellular carcinoma; Carcinoma prognosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032

Contact force-guided catheter ablation for the treatment of atrial fibrillation: a meta-analysis of randomized, controlled trials
Provedor de dados: 56
Autores: Qi,Z.; Luo,X.; Wu,B.; Shi,H.; Jin,B.; Wen,Z.
Contact force (CF) sensing technology allows real-time monitoring during catheter ablation for atrial fibrillation (AF). However, the effect of CF sensing technology on procedural parameters and clinical outcomes still needs clarification. Because of the inconsistent results thus far in this area, we performed a meta-analysis to determine whether CF sensing technology can improve procedural parameters and clinical outcomes for the treatment of AF. Studies examining the benefits of CF sensing technology were identified in English-language articles by searching the MEDLINE, Web of Science, and Cochrane Library databases (inception to May 2015). Ten randomized, controlled trials involving 1834 patients (1263 males, 571 females) were included in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atrial fibrillation Contact force sensing Meta-analysis Randomized; Controlled trials.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300707

Different stress modalities result in distinct steroid hormone responses by male rats
Provedor de dados: 56
Autores: Andersen,M.L.; Bignotto,M.; Machado,R.B.; Tufik,S.
Since both paradoxical sleep deprivation (PSD) and stress alter male reproductive function, the purpose of the present study was to examine the influence of PSD and other stressors (restraint, electrical footshock, cold and forced swimming, N = 10 per group) on steroid hormones in adult Wistar male rats. Rats were submitted to chronic stress for four days. The stressors (footshock, cold and forced swimming) were applied twice a day, for periods of 1 h at 9:00 and 16:00 h. Restrained animals were maintained in plastic cylinders for 22 h/day whereas PSD was continuous. Hormone determination was measured by chemiluminescent enzyme immunoassay (testosterone), competitive immunoassay (progesterone) and by radioimmunoassay (corticosterone, estradiol, estrone)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paradoxical sleep deprivation; Stress; Testosterone; Progesterone; Estrogen; Corticosterone.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600003

Brain caspase-3 and intestinal FABP responses in preterm and term rats submitted to birth asphyxia
Provedor de dados: 56
Autores: Figueira,R.L.; Gonçalves,F.L.; Simões,A.L.; Bernardino,C.A.; Lopes,L.S.; Castro e Silva,O.; Sbragia,L.
Neonatal asphyxia can cause irreversible injury of multiple organs resulting in hypoxic-ischemic encephalopathy and necrotizing enterocolitis (NEC). This injury is dependent on time, severity, and gestational age, once the preterm babies need ventilator support. Our aim was to assess the different brain and intestinal effects of ischemia and reperfusion in neonate rats after birth anoxia and mechanical ventilation. Preterm and term neonates were divided into 8 subgroups (n=12/group): 1) preterm control (PTC), 2) preterm ventilated (PTV), 3) preterm asphyxiated (PTA), 4) preterm asphyxiated and ventilated (PTAV), 5) term control (TC), 6) term ventilated (TV), 7) term asphyxiated (TA), and 8) term asphyxiated and ventilated (TAV). We measured body, brain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal asphyxia; Necrotizing enterocolitis; I-FABP; Caspase-3; Brain injury; Intestinal damage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700703

Low-dose thioperamide injected into the cerebellar vermis of mice immediately after exposure to the elevated plus-maze impairs their avoidance behavior on re-exposure to the apparatus
Provedor de dados: 56
Autores: Costa Neto,J.; Serafim,K.R.; Gianlorenço,A.C.L.; Mattioli,R.
The present study investigated the effect of thioperamide (THIO), an H3 histaminergic receptor antagonist, microinjected into the cerebellar vermis on emotional memory consolidation in male Swiss albino mice re-exposed to the elevated plus-maze (EPM). We implanted a guide cannula into the cerebellar vermis using stereotactic surgery. On the third day after surgery, we performed behavioral tests for two consecutive days. On the first day (exposure), the mice (n=10/group) were exposed to the EPM and received THIO (0.06, 0.3, or 1.5 ng/0.1 µL) immediately after the end of the session. Twenty-four hours later, the mice were re-exposed to the EPM under the same experimental conditions, but without drug injection. A reduction in the exploration of the open arms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histaminergic system; Cerebellum; H3; Emotional memory; Memory consolidation; Elevated plus-maze.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100943

POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells
Provedor de dados: 56
Autores: França,M. M.; Abreu,N. P.; Vrechi,T. A. M.; Lotfi,C. F.
During gonad and adrenal development, the POD-1/capsulin/TCF21transcription factor negatively regulates SF-1/NR5A1expression, with higher SF-1 levels being associated with increased adrenal cell proliferation and tumorigenesis. In adrenocortical tumor cells, POD-1 binds to the SF-1 E-box promoter region, decreasing SF-1 expression. However, the modulation of SF-1 expression by POD-1 has not previously been described in normal adrenal cells. Here, we analyzed the basal expression of Pod-1 and Sf-1 in primary cultures of glomerulosa (G) and fasciculata/reticularis (F/R) cells isolated from male Sprague-Dawley rats, and investigated whether POD-1 overexpression modulates the expression of endogenous Sf-1 and its target genes in these cells. POD-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: POD-1; Tcf21; SF-1; Rat adrenal cell primary culture; Glomerulosa cells; Fasciculata cells.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201087

Monoamines in the pedal plexus of the land snail Megalobulimus oblongus (Gastropoda, Pulmonata)
Provedor de dados: 56
Autores: Faccioni-Heuser,M.C.; Zancan,D.M.; Achaval,M.
In molluscs, the number of peripheral neurons far exceeds those found in the central nervous system. Although previous studies on the morphology of the peripheral nervous system exist, details of its organization remain unknown. Moreover, the foot of the terrestrial species has been studied less than that of the aquatic species. As this knowledge is essential for our experimental model, the pulmonate gastropod Megalobulimus oblongus, the aim of the present study was to investigate monoamines in the pedal plexus of this snail using two procedures: glyoxylic acid histofluorescence to identify monoaminergic structures, and the unlabeled antibody peroxidase anti-peroxidase method using antiserum to detect the serotonergic component of the plexus. Adult land...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pedal plexus; Catecholamines; Serotonin; Glyoxylic acid; Immunohistochemistry; Gastropod.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700014

Magnesium chloride alone or in combination with diazepam fails to prevent hippocampal damage following transient forebrain ischemia
Provedor de dados: 56
Autores: Milani,H.; Lepri,E.R.; Giordani,F.; Favero-Filho,L.A.
In the central nervous system, magnesium ion (Mg2+) acts as an endogenous modulator of N-methyl-D-aspartate (NMDA)-coupled calcium channels, and may play a major role in the pathomechanisms of ischemic brain damage. In the present study, we investigated the effects of magnesium chloride (MgCl2, 2.5, 5.0 or 7.5 mmol/kg), either alone or in combination with diazepam (DZ), on ischemia-induced hippocampal cell death. Male Wistar rats (250-300 g) were subjected to transient forebrain ischemia for 15 min using the 4-vessel occlusion model. MgCl2 was applied systemically (sc) in single (1x, 2 h post-ischemia) or multiple doses (4x, 1, 2, 24 and 48 h post-ischemia). DZ was always given twice, at 1 and 2 h post-ischemia. Thus, ischemia-subjected rats were assigned...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia; Hippocampal lesion; CA1 cell loss; Magnesium chloride; Diazepam; Neuroprotection.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000016

Biological activity and binding of estradiol to SK-Mel 23 human melanoma cells
Provedor de dados: 56
Autores: Sarti,M.S.M.V.; Visconti,M.A.; Castrucci,A.M.L.
Patients expressing estradiol receptors in melanoma cells have been reported to have a better prognosis. We therefore decided to investigate the in vitro effects of ß-estradiol and tamoxifen on the growth and tyrosinase activity of SK-Mel 23 human melanoma cells. Twenty-four-hour treatment with 0.4 nM ß-estradiol inhibited cell proliferation in 30% (0.70 ± 0.03 x 10(5) cells) and increased tyrosinase activity in 50% (7130.5 ± 376.5 cpm/10(5) cells), as compared to untreated cells (1.0 ± 0.05 x 10(5) cells and 4769 ± 25.5 cpm/10(5) cells, respectively). Both responses were completely (100%) blocked by 1 µM tamoxifen. Higher concentrations (up to 1.6 nM) or longer treatments (up to 72 h) did not result in a larger effect of the hormone on proliferation or...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SK-Mel 23 cell line; Human melanoma; SS-estradiol; Tamoxifen; Cell proliferation; Tyrosinase activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600016

Editorial
Provedor de dados: 56
Autores: Teixeira,M.M.; Cunha,F.Q.; Ferreira,S.H.
Tipo: Info:eu-repo/semantics/other
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500001

Transduction motif analysis of gastric cancer based on a human signaling network
Provedor de dados: 56
Autores: Liu,G.; Li,D.Z.; Jiang,C.S.; Wang,W.
To investigate signal regulation models of gastric cancer, databases and literature were used to construct the signaling network in humans. Topological characteristics of the network were analyzed by CytoScape. After marking gastric cancer-related genes extracted from the CancerResource, GeneRIF, and COSMIC databases, the FANMOD software was used for the mining of gastric cancer-related motifs in a network with three vertices. The significant motif difference method was adopted to identify significantly different motifs in the normal and cancer states. Finally, we conducted a series of analyses of the significantly different motifs, including gene ontology, function annotation of genes, and model classification. A human signaling network was constructed,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Significantly different motifs; Human signaling network; Gastric cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500369

Animal model for age- and sex-related genotoxicity of diethylstilbestrol
Provedor de dados: 56
Autores: Fučić,A.; Stojkovic,R.; Katić,J.; Markovic,D.; Ferencic,Z.; Koršić,M.; Jazbec,A.M.; Gamulin,M.
Environmental xenoestrogens pose a significant health risk for all living organisms. There is growing evidence concerning the different susceptibility to xenoestrogens of developing and adult organisms, but little is known about their genotoxicity in pre-pubertal mammals. In the present study, we developed an animal model to test the sex- and age-specific genotoxicity of the synthetic estrogen diethylstilbestrol (DES) on the reticulocytes of 3-week-old pre-pubertal and 12-week-old adult BALB/CJ mice using the in vivo micronucleus (MN) assay. DES was administered intraperitoneally at doses of 0.05, 0.5, and 5 µg/kg for 3 days and animals were sampled 48, 72 and 96 h, and 2 weeks after exposure. Five animals were analyzed for each dose, sex, and age group....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diethylstilbestrol; Estrogen; Puberty; In vivo micronucleus assay; Sex; Genotoxicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100015

Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells
Provedor de dados: 56
Autores: Loopuijt,L.D.; Silva Filho,M. da; Hirt,B.; Vonthein,R.; Kremers,J.
To study the dendritic morphology of retinal ganglion cells in wild-type mice we intracellularly injected these cells with Lucifer yellow in an in vitro preparation of the retina. Subsequently, quantified values of dendritic thickness, number of branching points and level of stratification of 73 Lucifer yellow-filled ganglion cells were analyzed by statistical methods, resulting in a classification into 9 groups. The variables dendritic thickness, number of branching points per cell and level of stratification were independent of each other. Number of branching points and level of stratification were independent of eccentricity, whereas dendritic thickness was positively dependent (r = 0.37) on it. The frequency distribution of dendritic thickness tended...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ganglion cell morphology; Retinal circuitry; Intracellular injection of neurotracers; Lucifer yellow; Dendritic thickness.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000010

Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
Provedor de dados: 56
Autores: Maranhão,A.Q.; Brígido,M.M.
We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple copies of the antibody fragment, and the others can be used to make monovalent libraries. The vectors use different promoter/leader sequences to direct the expression of the fused proteins. All were able to promote the assembly of fusion virion particles. In this paper we also show the affinity selection (biopanning) of those phage-antibodies based on the capacity of their products to recognize the antigen. We used biotinylated Z-DNA and the selection was performed in a solution phase fashion. The data presented here indicate that these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-nucleic acid antibodies; Phage display; Biopanning; ScFv; Z-DNA.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500012

Stabilization of serum antibody responses triggered by initial mucosal contact with the antigen independently of oral tolerance induction
Provedor de dados: 56
Autores: Verdolin,B.A.; Ficker,S.M.; Faria,A.M.C.; Vaz,N.M.; Carvalho,C.R.
Initial contacts with a T-dependent antigen by mucosal routes may result in oral tolerance, defined as the inhibition of specific antibody formation after subsequent parenteral immunizations with the same antigen. We describe here an additional and permanent consequence of these initial contacts, namely, the blockade of secondary-type responsiveness to subsequent parenteral contacts with the antigen. When repeatedly boosted ip with small doses (3 µg) of ovalbumin (OVA) (or lysozyme), primed B6D2F1 mice showed progressively higher antibody responses. In contrast, mice primed after a single oral exposure to the antigen, although repeatedly boosted, maintained their secondary antibody titers on a level which was inversely proportional to the dose of antigen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral tolerance; Mucosal immunity; Stability; Memory.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200008

Reactivity of the isolated perfused rat tail vascular bed
Provedor de dados: 56
Isolated segments of the perfused rat tail artery display a high basal tone when compared to other isolated arteries such as the mesenteric and are suitable for the assay of vasopressor agents. However, the perfusion of this artery in the entire tail has not yet been used for functional studies. The main purpose of the present study was to identify some aspects of the vascular reactivity of the rat tail vascular bed and validate this method to measure vascular reactivity. The tail severed from the body was perfused with Krebs solution containing different Ca2+ concentrations at different flow rates. Rats were anesthetized with sodium pentobarbital (65 mg/kg) and heparinized (500 U). The tail artery was dissected near the tail insertion, cannulated and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rat tail vascular bed; Phenylephrine; Calcium; Flow; Endothelium; Vascular tone; Perfusion pressure.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700012

Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization
Provedor de dados: 56
Autores: Persechini,P.M.; Bisaggio,R.C.; Alves-Neto,J.L.; Coutinho-Silva,R.
The effects of extracellular nucleosides and nucleotides on many organs and systems have been recognized for almost 50 years. The effects of extracellular ATP (ATPo), UTPo, ADPo, and other agonists are mediated by P2 purinoceptors. One of the most dramatic effects of ATPo is the permeabilization of plasma membranes to low molecular mass solutes of up to 900 Da. This effect is evident in several cells of the lymphohematopoietic system and is supposed to be mediated by P2Z, an ATP4--activated purinoceptor. Here, we review some basic information concerning P2 purinoceptors and focus our attention on P2Z-associated phenomena displayed by macrophages. Using fluorescent dye uptake, measurement of free intracellular Ca2+ concentration and electrophysiological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ATP; P2Z purinoceptor; Macrophage; Monocyte; T cell; Permeabilization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100004

Pup age and aggressive behavior in lactating rats
Provedor de dados: 56
Autores: Giovenardi,M.; Consiglio,A.R.; Barros,H.M.T.; Lucion,A.B.
High levels of aggressive behaviors against intruders in the nest area are displayed by female rats during the first 10 days after delivery, declining thereafter to very low levels, even though lactation continues. Cross-fostering experiments were undertaken to test the hypothesis that pup age may affect aggression in lactating rats. The behavior of females on the 8th day after delivery when raising fostered 8-day-old pups was compared to that of females on the 8th postpartum day raising older pups (18 days old) for the last 5 days, and females on the 18th day after delivery raising fostered 18-day-old pups were compared to females in the same postpartum period nursing younger pups (8 days of age at the time of the maternal aggression test) for 5 days. Pup...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maternal aggression; Mother-infant interaction; Cross-fostering; Prolactin.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900015

Histomorphometric analysis of the response of rat skeletal muscle to swimming, immobilization and rehabilitation
Provedor de dados: 56
Autores: Nascimento,C.C.F.; Padula,N.; Milani,J.G.P.O.; Shimano,A.C.; Martinez,E.Z.; Mattiello-Sverzut,A.C.
The objective of the present study was to determine to what extent, if any, swimming training applied before immobilization in a cast interferes with the rehabilitation process in rat muscles. Female Wistar rats, mean weight 260.52 ± 16.26 g, were divided into 4 groups of 6 rats each: control, 6 weeks under baseline conditions; trained, swimming training for 6 weeks; trained-immobilized, swimming training for 6 weeks and then immobilized for 1 week; trained-immobilized-rehabilitated, swimming training for 6 weeks, immobilized for 1 week and then remobilized with swimming for 2 weeks. The animals were then sacrificed and the soleus and tibialis anterior muscles were dissected, frozen in liquid nitrogen and processed histochemically (H&amp;E and mATPase)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swimming training; Female rat skeletal muscles; Immobilization; Morphometry; ATPase.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900013

Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
Provedor de dados: 56
Autores: Aragão,T.C.F.R.; Souza,P.A.S.; Uchôa,A.F.; Costa,I.R.; Bloch Jr.,C.; Campos,F.A.P.
We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactaceae; Mandacaru; Cereus jamacaru; Cactin; 2S albumins; Methionine-rich protein.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800005

Correlates of C-reactive protein levels in young adults: a population-based cohort study of 3827 subjects in Brazil
Provedor de dados: 56
Autores: Nazmi,A.; Oliveira,I.O.; Victora,C.G.
The socio-demographic, behavioral and anthropometric correlates of C-reactive protein levels were examined in a representative young adult Brazilian population. The 1982 Pelotas Birth Cohort Study (Brazil) recruited over 99% of births in the city of Pelotas that year (N = 5914). Individuals belonging to the cohort have been prospectively followed up. In 2004-2005, 77.4% of the cohort was traced, members were interviewed and 3827 individuals donated blood. Analyses of the outcome were based on a conceptual model that differentiated confounders from potential mediators. The following independent variables were studied in relation to levels of C-reactive protein in sex-stratified analyses: skin color, age, family income, education, parity, body mass index,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-reactive protein; Inflammation; Cohort studies; Prospective studies; Brazil.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500003

Viability of mesenchymal stem cells during electrospinning
Provedor de dados: 56
Autores: Zanatta,G.; Steffens,D.; Braghirolli,D.I.; Fernandes,R.A.; Netto,C.A.; Pranke,P.
Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanotechnology; Tissue engineering; Electrospinning; Stem cells.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200006

Proteomic analysis of cytosolic proteins associated with petite mutations in Candida glabrata
Provedor de dados: 56
Autores: Loureiro y Penha,C.V.; Kubitschek,P.H.B.; Larcher,G.; Perales,J.; Rodriguez León,I.; Lopes-Bezerra,L.M.; Bouchara,J.P.
The incidence of superficial or deep-seated infections due to Candida glabrata has increased markedly, probably because of the low intrinsic susceptibility of this microorganism to azole antifungals and its relatively high propensity to acquire azole resistance. To determine changes in the C. glabrata proteome associated with petite mutations, cytosolic extracts from an azole-resistant petite mutant of C. glabrata induced by exposure to ethidium bromide, and from its azole-susceptible parent isolate were compared by two-dimensional polyacrylamide gel electrophoresis. Proteins of interest were identified by peptide mass fingerprinting or sequence tagging using a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer. Tryptic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida glabrata; Petite mutations; Cytosolic extracts; Proteomic analysis; Azole resistance.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200010

Factor structure of Bech's version of the Brief Psychiatric Rating Scale in Brazilian patients
Provedor de dados: 56
Autores: Crippa,J.A.S.; Sanches,R.F.; Hallak,J.E.C.; Loureiro,S.R.; Zuardi,A.W.
The objective of the present study was to evaluate the factor structure of Bech's version of the Brief Psychiatric Rating Scale (BPRS), translated into Portuguese. The BPRS was administered to a heterogeneous group of psychiatric inpatients (N = 98) and outpatients (N = 62) in a University Hospital. Each patient was evaluated from one to eight times. The interval between consecutive interviews was one week for the inpatients and one month for the outpatients. The results were submitted to factorial analysis. The internal consistency of the total scale and of each factor was also estimated. Factorial analysis followed by normalized orthogonal rotation (Varimax) yielded four factors: Withdrawal-Retardation, Thinking Disorder, Anxious-Depression and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Brief Psychiatric Rating Scale; Psychiatric status rating scales; Psychometrics; Portuguese version; Factorial analysis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000014

Frequency of islet cell autoantibodies (IA-2 and GAD) in young Brazilian type 1 diabetes patients
Provedor de dados: 56
Autores: Pardini,V.C.; Mourão,D.M.; Nascimento,P.D.; Vívolo,M.A.; Ferreira,S.R.G.; Pardini,H.
Type 1 diabetes, as an autoimmune disease, presents several islet cell-specific autoantibodies such as islet cell antibody (ICA), anti-insulin, anti-glutamic acid decarboxylase (GAD) and the antibody (Ab) against tyrosine phosphatase (PTP)-like protein known as ICA-512 (IA-2). In order to determine the frequency of the anti-GAD and anti-IA-2 autoantibodies in Brazilian type 1 diabetes patients we studied 35 diabetes mellitus (DM) type 1 patients with recent-onset disease (&lt;FONT FACE="Symbol"&gt;£&lt;/font&gt;12 months) and 37 type 1 diabetes patients with long-duration diabetes (&amp;gt;12 months) who were compared to 12 children with normal fasting glucose. Anti-GAD65 and anti-IA-2 autoantibodies were detected with commercial immunoprecipitation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Type 1 diabetes; Autoimmunity; GAD and IA-2 autoantibodies.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000003

Anandamide injected into the lateral ventricle of the brain inhibits submandibular salivary secretion by attenuating parasympathetic neurotransmission
Provedor de dados: 56
Autores: Fernandez-Solari,J.; Prestifilippo,J.P.; Vissio,P.; Ehrhart-Bornstein,M.; Bornstein,S.R.; Rettori,V.; Elverdin,J.C.
Our objective was to determine the effect of arachidonylethanolamide (anandamide, AEA) injected intracerebroventricularly (icv) into the lateral ventricle of the rat brain on submandibular gland (SMG) salivary secretion. Parasympathetic decentralization (PSD) produced by cutting the chorda tympani nerve strongly inhibited methacholine (MC)-induced salivary secretion while sympathetic denervation (SD) produced by removing the superior cervical ganglia reduced it slightly. Also, AEA (50 ng/5 µL, icv) significantly decreased MC-induced salivary secretion in intact rats (MC 1 µg/kg: control (C), 5.3 ± 0.6 vs AEA, 2.7 ± 0.6 mg; MC 3 µg/kg: C, 17.6 ± 1.0 vs AEA, 8.7 ± 0.9 mg; MC 10 µg/kg: C, 37.4 ± 1.2 vs AEA, 22.9 ± 2.6 mg). However, AEA did not alter the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anandamide; Parasympathetic nervous system; Submandibular gland; Cannabinoid receptor type 1; Gamma-aminobutyric acid.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600010

Age-related changes and effects of regular low-intensity exercise on gait, balance, and oxidative biomarkers in the spinal cord of Wistar rats
Provedor de dados: 56
Autores: Silveira,E.M.S.; Kroth,A.; Santos,M.C.Q.; Silva,T.C.B.; Silveira,D.; Riffel,A.P.K.; Scheid,T.; Trapp,M.; Partata,W.A.
The present study aimed to analyze age-related changes to motor coordination, balance, spinal cord oxidative biomarkers in 3-, 6-, 18-, 24-, and 30-month-old rats. The effects of low-intensity exercise on these parameters were also analyzed in 6-, 18-, and 24-month-old rats. Body weight, blood glucose, total cholesterol, and high-density lipoprotein (HDL) cholesterol were assessed for all rats. The soleus muscle weight/body weight ratio was used to estimate skeletal muscle mass loss. Body weight increased until 24 months; only 30-month-old rats exhibited decreased blood glucose and increased total cholesterol and HDL cholesterol. The soleus muscle weight/body weight ratio increased until 18 months, followed by a small decrease in old rats. Exercise did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide anion generation; Hydrogen peroxide; Total thiol content; Lipid hydroperoxide levels; Total antioxidant capacity; Superoxide dismutase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700608

Construction of a fusion plasmid containing the PSCA gene and cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and its anti-tumor effect in an animal model of prostate cancer
Provedor de dados: 56
Autores: Mai,T.J.; Ma,R.; Li,Z.; Bi,S.C.
Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a negative regulator of T cell activation, which competes with CD28 for B7.1/B7.2 binding, and which has a greater affinity. Fusion of specific antigens to extracellular domain of CTLA4 represents a promising approach to increase the immunogenicity of DNA vaccines. In this study, we evaluated this interesting approach for CTLA4 enhancement on prostate stem cell antigen (PSCA)-specific immune responses and its anti-tumor effects in a prostate cancer mouse model. Consequently, we constructed a DNA vaccine containing the PSCA and the CTLA-4 gene. Vaccination with the CTLA4-fused DNA not only induced a much higher level of anti-PSCA antibody, but also increased PSCA-specific T cell response in mice. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; DNA vaccine; PSCA; CTLA-4.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100601

Effect of injection of L-NAME on drinking response
Provedor de dados: 56
Autores: Saad,W.A.; Camargo,L.A.A.; Saad,R.; Pereira,A.F.; Simões,S.
The drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water intake increased in all animals after angiotensin II (ANG II) injection into the LV, with values of 14.2 ± 1.4 ml/h. After injection of L-NAME at doses of 10, 20 or 40 µg/µl into the SFO before injection of ANG II (12 ng/µl) into the LV, water intake decreased progressively and reached basal levels after treatment with 0.15 M NaCl and with the highest dose of L-NAME (i.e., 40 µg). The water intake obtained after 40 µg/µl L-NAME was 0.8 ± 0.01 ml/h. Also, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Water intake; SFO; Dehydration; CNS.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100012

Food restriction induces in vivo ventricular dysfunction in spontaneously hypertensive rats without impairment of in vitro myocardial contractility
Provedor de dados: 56
Autores: Okoshi,K.; Fioretto,J.R.; Okoshi,M.P.; Cicogna,A.C.; Aragon,F.F.; Matsubara,L.S.; Matsubara,B.B.
Cardiac structures, function, and myocardial contractility are affected by food restriction (FR). There are few experiments associating undernutrition with hypertension. The aim of the present study was to analyze the effects of FR on the cardiac response to hypertension in a genetic model of hypertension, the spontaneously hypertensive rat (SHR). Five-month-old SHR were fed a control or a calorie-restricted diet for 90 days. Global left ventricle (LV) systolic function was evaluated in vivo by transthoracic echocardiogram and myocardial contractility and diastolic function were assessed in vitro in an isovolumetrically beating isolated heart (Langendorff preparation). FR reduced LV systolic function (control (mean ± SD): 58.9 ± 8.2; FR: 50.8 ± 4.8%, N =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Undernutrition; Isolated heart; Echocardiogram; Langendorff preparation; Ventricular remodeling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400019

Is avoidant disorder part of the social phobia spectrum in a referred sample of Brazilian children and adolescents?
Provedor de dados: 56
Autores: Denardin,D.; Silva,T.L.; Pianca,T.G.; Rohde,L.A.
The diagnosis of avoidant disorder was deleted from the Diagnostic and Statistical Manual of Mental disorders - fourth edition (DSM-IV) based on a `committee decision' suggesting that avoidant disorder is part of the social phobia spectrum. The objective of the present study was to examine the nature of this clinical association in a referred sample of Brazilian children and adolescents. We assessed a referred sample of 375 youths using semi-structured diagnostic interview methodology. Demographic (age at admission to the study and sex) and clinical (level of impairment, age at onset of symptoms and pattern of comorbidity) data were assessed in subsamples of children with avoidant disorder (N = 7), social phobia (N = 26), and comorbidity between both...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anxiety disorders; Avoidant disorder; Social phobia; Mental disorder diagnosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600012

Classical and recent advances in the treatment of inflammatory bowel diseases
Provedor de dados: 56
Autores: Sales-Campos,H.; Basso,P.J.; Alves,V.B.F.; Fonseca,M.T.C.; Bonfá,G.; Nardini,V.; Cardoso,C.R.B.
Crohn's disease (CD) and ulcerative colitis (UC) are intestinal disorders that comprise the inflammatory bowel diseases (IBD). These disorders have a significant effect on the quality of life of affected patients and the increasing number of IBD cases worldwide is a growing concern. Because of the overall burden of IBD and its multifactorial etiology, efforts have been made to improve the medical management of these inflammatory conditions. The classical therapeutic strategies aim to control the exacerbated host immune response with aminosalicylates, antibiotics, corticosteroids, thiopurines, methotrexate and anti-tumor necrosis factor (TNF) biological agents. Although successful in the treatment of several CD or UC conditions, these drugs have limited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammatory bowel disease; Crohn's disease; Ulcerative colitis; Treatment; Immune modulation; Cellular therapy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200096

Heparan sulphate, its derivatives and analogues share structural characteristics that can be exploited, particularly in inhibiting microbial attachment
Provedor de dados: 56
Autores: Rudd,T.R.; Hughes,A.; Holman,J.; Solari,V.; de Oliveira Ferreira,E.; Cavalcante Pilotto Domingues,R.M.; Yates,E.A.
Heparan sulphate (HS) and the related polysaccharide, heparin, exhibit conformational and charge arrangement properties, which provide a degree of redundancy allowing several seemingly distinct sequences to exhibit the same activity. This can also be mimicked by other sulphated polysaccharides, both in overall effect and in the details of interactions and structural consequences of interactions with proteins. Together, these provide a source of active compounds suitable for further development as potential drugs. These polysaccharides also possess considerable size, which bestows upon them an additional useful property: the capability of disrupting processes comprising many individual interactions, such as those characterising the attachment of microbial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparan sulfate; Biosynthesis; Structure-function; Microbial attachment.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500002

Plasma brain-derived neurotrophic factor is higher after combat training (Randori) than incremental ramp test in elite judo athletes
Provedor de dados: 56
Autores: Schor,B.; Silva,S. Gomes da; Almeida,A.A. de; Pereira,C.A.B.; Arida,R.M.
Elite judo demands high levels of physical and psychological skills. The brain-derived neurotrophic factor (BDNF) may be of particular interest in sports medicine for its ability to promote neuroplasticity. We investigated the plasma BDNF before and after a judo training session (Randori) and the maximal incremental ramp test (MIRT) in athletes from the Brazilian national judo team and compared the results between both exercise stimuli and sexes. Fifty-six elite judo athletes were recruited who performed each task on a separated day. Anthropometric, physiological, athletic parameters, and plasma BDNF levels were measured before and after the tasks (Randori and MIRT). The groups presented similar anthropometric and physiological characteristics at baseline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Judo; BDNF; Elite athletes; Neuroplasticity; Randori; Exercise.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400601

Social condition affects hormone secretion and exploratory behavior in rats
Provedor de dados: 56
Autores: Genaro,G.; Schmidek,W.R.; Franci,C.R.
Studies of behavior, endocrinology and physiology have described experiments in which animals housed in groups or in isolation were normally tested individually. The isolation of the animal from its group for testing is perhaps the most common situation used today in experimental procedures, i.e., there is no consideration of the acute stress which occurs when the animal is submitted to a situation different from that it is normally accustomed to, i.e., group living. In the present study, we used 90 male 120-day-old rats (Rattus norvegicus) divided into 5 groups of 18 animals, which were housed 3 per cage, in a total of 6 cages. The animals were tested individually or with their groups for exploratory behavior. Hormones were determined by radioimmunoassay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Group; Stress; Reproduction; ACTH; Luteinizing hormone; Corticosterone; Prolactin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600008

Simultaneous changes in the function and expression of beta 1 integrins during the growth arrest of poorly differentiated colorectal cells (LISP-1)
Provedor de dados: 56
Autores: Roela,R.A.; Brentani,M.M.; Katayama,M.L.H.; Reis,M.; Federico,M.H.H.
Cells usually lose adhesion and increase proliferation and migration during malignant transformation. Here, we studied how proliferation can affect the other two characteristics, which ultimately lead to invasion and metastasis. We determined the expression of ß1 integrins, as well as adhesion and migration towards laminin-1, fibronectin, collagens type I and type IV presented by LISP-1 colorectal cancer cells exposed to 2.5% dimethyl sulfoxide (DMSO), an agent capable of decreasing proliferation in this poorly differentiated colorectal cell line. Untreated cells (control), as shown by flow cytometry and monoclonal antibodies, expressed alpha2 (63.8 ± 11.3% positive cells), alpha3 (93.3 ± 7.0%), alpha5 (50.4 ± 12.0%) and alpha6 (34.1 ± 4.9%) integrins but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell adhesion molecules; Cell migration; Integrin; Cell cycle; DMSO.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800016

Cell therapy in dilated cardiomyopathy: back to the right scientific track?
Provedor de dados: 56
Autores: Marin-Neto,J.A.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600001

Reversal of the anticoagulant and anti-hemostatic effect of low molecular weight heparin by direct prothrombin activation
Provedor de dados: 56
Autores: Andrade,S.A.; Carrijo-Carvalho,L.C.; Peceguini,L.A.M.; Wlian,L.; Sato,A.C.; Luchiari,C.; Silva,E.D.; Maffei,F.H.A.; Chudzinski-Tavassi,A.M.
Lopap, found in the bristles of Lonomia obliqua caterpillar, is the first exogenous prothrombin activator that shows serine protease-like activity, independent of prothrombinase components and unique lipocalin reported to interfere with hemostasis mechanisms. To assess the action of an exogenous prothrombin activator reversing the anticoagulant and antihemostatic effect induced by low molecular weight heparin (LMWH), male New Zealand rabbits (N = 20, weighing 3.8-4.0 kg) allocated to 4 groups were anticoagulated with 1800 IU/kg LMWH (iv) over 2 min, followed by iv administration of saline (SG) or recombinant Lopap (rLopap) at 1 µg/kg (LG1) or 10 µg/kg (LG10), 10 min after the injection of LMWH, in a blind manner. Control animals (CG) were treated only with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prothrombin activation; Procoagulant; Exogenous hemostatic factor; RLopap; Bleeding; Anticoagulant.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000007

Silencing HIF-1α reduces the adhesion and secretion functions of acute leukemia hBMSCs
Provedor de dados: 56
Autores: Dong-Feng,Zeng; Ting,Liu; Cheng,Chang; Xi,Zhang; Xue,Liang; Xing-Hua,Chen; Pei-Yan,Kong.
Hypoxia inducible factor-1α (HIF-1α) is an important transcription factor, which plays a critical role in the formation of solid tumor and its microenviroment. The objective of the present study was to evaluate the expression and function of HIF-1α in human leukemia bone marrow stromal cells (BMSCs) and to identify the downstream targets of HIF-1α. HIF-1α expression was detected at both the RNA and protein levels using real-time PCR and immunohistochemistry, respectively. Vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) were detected in stromal cells by enzyme-linked immunosorbent assay. HIF-1α was blocked by constructing the lentiviral RNAi vector system and infecting the BMSCs. The Jurkat cell/BMSC co-cultured system...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIF-1α; Human bone marrow stromal cells (hBMSCs); Leukemia; RNAi; SDF-1α; VEGF.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000004

Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet
Provedor de dados: 56
Autores: Barros,L.A.; Costa-Silva,M.; Biolchini,C.L.; Neves,R.H.; Machado-Silva,J.R.
A study was undertaken to investigate the effect of administering praziquantel (PZQ), focusing on the liver stereological findings of malnourished mice infected with Schistosoma mansoni. Thirty female Swiss Webster mice (age: 21 days; weight: 8-14 g) were fed either a low-protein diet (8%) or standard chow (22% protein) for 15 days. Five mice in each group were infected with 50 cercariae each of the BH strain (Brazil). PZQ therapy (80 mg/kg body weight, per day) was started on the 50th day of infection and consisted of daily administration for 5 days. Volume density (hepatocytes, sinusoids and hepatic fibrosis) was determined by stereology using a light microscope. Body weight gain and total serum albumin levels were always lower in undernourished mice....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Schistosoma mansoni; Low-protein diet; Stereology; Praziquantel.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900007

Climatic changes: what if the global increase of CO2 emissions cannot be kept under control?
Provedor de dados: 56
Autores: Barreto de Castro,L.A.
Climatic changes threaten the planet. Most articles related to the subject present estimates of the disasters expected to occur, but few have proposed ways to deal with the impending menaces. One such threat is the global warming caused by the continuous increase in CO2 emissions leading to rising ocean levels due to the increasing temperatures of the polar regions. This threat is assumed to eventually cause the death of hundreds of millions of people. We propose to desalinize ocean water as a means to reduce the rise of ocean levels and to use this water for populations that need good quality potable water, precisely in the poorest regions of the planet. Technology is available in many countries to provide desalinated water at a justifiable cost...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Climatic changes; CO2 emissions; Global warming; Desalination of ocean water.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300002

Correlation between lactose absorption and the C/T-13910 and G/A-22018 mutations of the lactase-phlorizin hydrolase (LCT) gene in adult-type hypolactasia
Provedor de dados: 56
Autores: Bulhões,A.C.; Goldani,H.A.S.; Oliveira,F.S.; Matte,U.S.; Mazzuca,R.B.; Silveira,T.R.
The C/T-13910 mutation is the major factor responsible for the persistence of the lactase-phlorizin hydrolase (LCT) gene expression. Mutation G/A-22018 appears to be only in co-segregation with C/T-13910. The objective of the present study was to assess the presence of these two mutations in Brazilian individuals with and without lactose malabsorption diagnosed by the hydrogen breath test (HBT). Ten milk-tolerant and 10 milk-intolerant individuals underwent the HBT after oral ingestion of 50 g lactose (equivalent to 1 L of milk). Analyses for C/T-13910 and G/A-22018 mutations were performed using a PCR-based method. Primers were designed for this study based on the GenBank sequence. The CT/GA, CT/AA, and TT/AA genotypes (lactase persistence) were found in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrogen breath test; Lactase-phlorizin hydrolase; Lactose intolerance; Milk intolerance; LCT polymorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100004

Pulmonary emphysema induced by passive smoking: an experimental study in rats
Provedor de dados: 56
Autores: Cendon,S.P.; Battlehner,C.; Lorenzi-Filho,G.; Dohlnikoff,M.; Pereira,P.M.; Conceição,G.M.S.; Beppu,O.S.; Saldiva,P.H.N.
We describe a short time model for inducing experimental emphysema in rats by chronic tobacco smoke inhalation. Three groups of male Wistar rats (6 months old) were studied: controls (N = 8), rats intoxicated for 45 days (s-45, N = 7) or for 90 days (s-90, N = 8). The exposed animals were intoxicated 3 times a day (10 cigarettes per exposure period), 5 days a week. Pulmonary damage was assessed by means of functional tests and quantitative pathological examination of the airways and lung parenchyma. The s-45 and s-90 animals were similar in terms of functional residual capacity (FRC) corrected for body weight (FRC/kg) but both groups of smoking rats exhibited significantly higher FRC/kg values than the controls (s-45 = 6.33; s-90 = 6.46; controls = 3.78;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passive smoking; Emphysema; Rats; Pulmonary function; Morphological analysis; Quantification.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000017

Evidence that blood pressure remains under the control of arterial baroreceptors in renal hypertensive rats
Provedor de dados: 56
Autores: Trindade Jr.,A.S.; Moreira,E.D.; Silva,G.J.J.; Krieger,E.M.
The purpose of the present study was to determine the range of the influence of the baroreflex on blood pressure in chronic renal hypertensive rats. Supramaximal electrical stimulation of the aortic depressor nerve and section of the baroreceptor nerves (sinoaortic denervation) were used to obtain a global analysis of the baroreceptor-sympathetic reflex in normotensive control and in chronic (2 months) 1-kidney, 1-clip hypertensive rats. The fall in blood pressure produced by electrical baroreceptor stimulation was greater in renal hypertensive rats than in normotensive controls (right nerve: -47 ± 8 vs -23 ± 4 mmHg; left nerve: -51 ± 7 vs -30 ± 4 mmHg; and both right and left nerves: -50 ± 8 vs -30 ± 4 mmHg; P &lt; 0.05). Furthermore, the increase in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Arterial baroreceptor; Renal hypertension; Sinoaortic denervation; Aortic depressor nerve; Electrical stimul.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000013

The mental health of graduate students at the Federal University of São Paulo: a preliminary report
Provedor de dados: 56
Autores: Nogueira-Martins,L.A.; Fagnani Neto,R.; Macedo,P.C.M.; Cítero,V.A.; Mari,J.J.
We present data regarding the care provided to graduate level health professionals at the mental health center of the Federal University of São Paulo. From September 1996 to September 2003, 146 graduate students (99 in the Master's degree program and 47 in the Doctoral program) were attended. This population was predominantly female (68.5%), with a mean (± SD) age of 28.6 ± 4.42 years, not married (71.9%). Most of the subjects were professionals who had not graduated from the Federal University (78.1%). The students who sought help for psychological and/or psychiatric problems were classified into two categories: situational-adaptive crises and psychopathological crises. The main diagnoses were depression and anxiety disorders (44%) causing 4.5% of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mental health; Graduate education; Burnout; Suicide; Occupational stress; Mental health program.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000011

Standardization of a computerized method for calculating autonomic function test responses in healthy subjects and patients with diabetes mellitus
Provedor de dados: 56
The objectives of the present study were 1) to compare results obtained by the traditional manual method of measuring heart rate (HR) and heart rate response (HRR) to the Valsalva maneuver, standing and deep breathing, with those obtained using a computerized data analysis system attached to a standard electrocardiograph machine; 2) to standardize the responses of healthy subjects to cardiovascular tests, and 3) to evaluate the response to these tests in a group of patients with diabetes mellitus (DM). In all subjects (97 healthy and 143 with DM) we evaluated HRR to deep breathing, HRR to standing, HRR to the Valsalva maneuver, and blood pressure response (BPR) to standing up and to a sustained handgrip. Since there was a strong positive correlation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetes mellitus; Cardiovascular autonomic neuropathy; Autonomic tests; Valsalva maneuver.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200007

Relationship between the actions of atrial natriuretic peptide (ANP), guanylin and uroguanylin on the isolated kidney
Provedor de dados: 56
Autores: Santos-Neto,M.S.; Carvalho,A.F.; Forte,L.R.; Fonteles,M.C.
Guanylin and uroguanylin are peptides that bind to and activate guanylate cyclase C and control salt and water transport in many epithelia in vertebrates, mimicking the action of several heat-stable bacteria enterotoxins. In the kidney, both of them have well-documented natriuretic and kaliuretic effects. Since atrial natriuretic peptide (ANP) also has a natriuretic effect mediated by cGMP, experiments were designed in the isolated perfused rat kidney to identify possible synergisms between ANP, guanylin and uroguanylin. Inulin was added to the perfusate and glomerular filtration rate (GFR) was determined at 10-min intervals. Sodium was also determined. Electrolyte dynamics were measured by the clearance formula. Guanylin (0.5 µg/ml, N = 12) or uroguanylin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ANP; Guanylin; Uroguanylin; Kidney; Natriuresis; CGMP.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800012

Clastogenic effects of different Ureaplasma urealyticum serovars on human chromosomes
Provedor de dados: 56
The possibility that Ureaplasma urealyticum might play an important role in human infertility was first raised more than 20 years ago, but this association remains speculative. Considering the hypothesis that the pathogenicity of Ureaplasma urealyticum may depend on its serotypes, the clastogenic effects of different strains of Ureaplasma urealyticum, at concentrations of 103 CCU (color changing units)/ml, 104 CCU/ml and 105 CCU/ml, were evaluated in vitro in short-term cultures of human lymphocytes. Total or partial mitotic inhibition was produced by Ureaplasma urealyticum serotypes 2, 3 and 10 independent of the concentration (103 CCU/ml, 104 CCU/ml or 105 CCU/ml) of the microorganisms employed. In contrast, the clastogenic effects observed with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mycoplasma; Chromosome alterations; Ureaplasma urealyticum; Clastogenic effects.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600008

The epigenetic modifiers 5-aza-2'-deoxycytidine and trichostatin A influence adipocyte differentiation in human mesenchymal stem cells
Provedor de dados: 56
Autores: Zych,J.; Stimamiglio,M.A.; Senegaglia,A.C.; Brofman,P.R.S.; Dallagiovanna,B.; Goldenberg,S.; Correa,A.
Epigenetic mechanisms such as DNA methylation and histone modification are important in stem cell differentiation. Methylation is principally associated with transcriptional repression, and histone acetylation is correlated with an active chromatin state. We determined the effects of these epigenetic mechanisms on adipocyte differentiation in mesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (ADSCs) using the chromatin-modifying agents trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2′-deoxycytidine (5azadC), a demethylating agent. Subconfluent MSC cultures were treated with 5, 50, or 500 nM TSA or with 1, 10, or 100 µM 5azadC for 2 days before the initiation of adipogenesis. The differentiation was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Trichostatin A; 5-Aza-2′-deoxycytidine; Adipocyte differentiation; Epigenetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500405

Cytokine expression patterns and mesenchymal stem cell karyotypes from the bone marrow microenvironment of patients with myelodysplastic syndromes
Provedor de dados: 56
Autores: Xiong,H.; Yang,X.Y.; Han,J.; Wang,Q.; Zou,Z.L.
The purpose of this study was to explore cytokine expression patterns and cytogenetic abnormalities of mesenchymal stem cells (MSCs) from the bone marrow microenvironment of Chinese patients with myelodysplastic syndromes (MDS). Bone marrow samples were obtained from 30 cases of MDS (MDS group) and 30 healthy donors (control group). The expression pattern of cytokines was detected by customized protein array. The karyotypes of MSCs were analyzed using fluorescence in situ hybridization. Compared with the control group, leukemia inhibitory factor, stem cell factor (SCF), stromal cell-derived factor (SDF-1), bone morphogenetic protein 4, hematopoietic stem cell (HSC) stimulating factor, and transforming growth factor-β in the MDS group were significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow microenvironment; Cytokine; Mesenchymal stem cell; Chromosome; Pathogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300207

Time motion analysis of football (soccer) referees during official matches in relation to the type of fluid consumed
Provedor de dados: 56
Autores: Da Silva,A.I.; Fernandes,L.C.; Fernandez,R.
The aim of the present study was to determine the effect of volume and composition of fluid replacement on the physical performance of male football referees. Ten referees were evaluated during three official matches. In one match the participants were asked to consume mineral water ad libitum, and in the others they consumed a pre-determined volume of mineral water or a carbohydrate electrolyte solution (6.4% carbohydrate and 22 mM Na+) equivalent to 1% of their baseline body mass (half before the match and half during the interval). Total water loss, sweat rate and match physiological performance were measured. When rehydrated ad libitum (pre-match and at half time) participants lost 1.97 ± 0.18% of their pre-match body mass (2.14 ± 0.19 L). This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Football referee; Dehydration; Sweat loss; Performance; Fluid replacement.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800010

Comparison of three methods for the determination of baroreflex sensitivity in conscious rats
Provedor de dados: 56
Autores: Farah,V.M.A.; Moreira,E.D.; Pires,M.D.; Irigoyen,M.C.C.; Krieger,E.M.
Baroreflex sensitivity was studied in the same group of conscious rats using vasoactive drugs (phenylephrine and sodium nitroprusside) administered by three different approaches: 1) bolus injection, 2) steady-state (blood pressure (BP) changes produced in steps), 3) ramp infusion (30 s, brief infusion). The heart rate (HR) responses were evaluated by the mean index (mean ratio of all HR changes and mean arterial pressure (MAP) changes), by linear regression and by the logistic method (maximum gain of the sigmoid curve by a logistic function). The experiments were performed on three consecutive days. Basal MAP and resting HR were similar on all days of the study. Bradycardic responses evaluated by the mean index (-1.5 ± 0.2, -2.1 ± 0.2 and -1.6 ± 0.2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex; Baroreceptor; Bradycardia; Tachycardia.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300018

Sleep habits and complaints of adults in the city of São Paulo, Brazil, in 1987 and 1995
Provedor de dados: 56
Autores: Pires,M.L.N.; Benedito-Silva,A.A.; Mello,M.T.; Del Giglio,S.; Pompeia,C.; Tufik,S.
This study compares the prevalence of complaints of insomnia, excessive diurnal sleepiness, parasomnias, and sleep habits of the adult population in the city of São Paulo, Brazil, estimated in surveys carried out in 1987 and 1995. Representative samples of 1000 adult residents per survey were interviewed using a validated structured sleep questionnaire, the "UNIFESP Sleep Questionnaire". Difficulty maintaining sleep, difficulty initiating sleep and early morning awakening, occurring at least three times a week, were reported in 1987 and 1995, by 15.8/27.6, 13.9/19.1, and 10.6/14.2% of the interviewees, respectively, significantly increasing throughout time. These sleep problems were more often found among women. Frequencies of excessive diurnal sleepiness...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep; Sleep disorders; Insomnia; Diurnal sleepiness; Parasomnias; Epidemiology; Sleep habits.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100011

The role of Ca2+/calmodulin-dependent protein kinase II and calcineurin in TNF-α-induced myocardial hypertrophy
Provedor de dados: 56
Autores: Wang,Gui-Jun; Wang,Hong-Xin; Yao,Yu-Sheng; Guo,Lian-Yi; Liu,Pei.
We investigated whether Ca2+/calmodulin-dependent kinase II (CaMKII) and calcineurin (CaN) are involved in myocardial hypertrophy induced by tumor necrosis factor α (TNF-α). The cardiomyocytes of neonatal Wistar rats (1-2 days old) were cultured and stimulated by TNF-α (100 μg/L), and Ca2+ signal transduction was blocked by several antagonists, including BAPTA (4 µM), KN-93 (0.2 µM) and cyclosporin A (CsA, 0.2 µM). Protein content, protein synthesis, cardiomyocyte volumes, [Ca2+]i transients, CaMKIIδB and CaN were evaluated by the Lowry method, [³H]-leucine incorporation, a computerized image analysis system, a Till imaging system, and Western blot analysis, respectively. TNF-α induced a significant increase in protein content in a dose-dependent manner...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial hypertrophy; Tumor necrosis factor α; Calcium; Calmodulin-dependent kinase; Calcineurin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100008

Effect of hepatitis C serology on C-reactive protein in a cohort of Brazilian hemodialysis patients
Provedor de dados: 56
Autores: Nascimento,M.M.; Bruchfeld,A.; Suliman,M.E.; Hayashi,S.Y.; Pecoits-Filho,R.; Manfro,R.C.; Pachaly,M.A.; Renner,L.; Stenvinkel,P.; Riella,M.C.; Lindholm,B.
Hepatitis C (HCV) is not an uncommon feature in hemodialysis (HD) patients and may be a cause of systemic inflammation. Plasma cytokine interleukin-6 (IL-6) is mainly produced by circulating and peripheral cells and induces the hepatic synthesis of C-reactive protein (CRP), which is the main acute phase reactant. The aim of this study was to investigate the influence of HCV on two markers of systemic inflammation, serum CRP and IL-6, in HD patients. The study included 118 HD patients (47% males, age 47 ± 13 years, 9% diabetics) who had been treated by standard HD for at least 6 months. The patients were divided into two groups depending on the presence (HCV+) or absence (HCV-) of serum antibodies against HCV. Serum albumin (S-Alb), plasma high sensitivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; C-reactive protein; Interleukin-6; Hemodialysis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500017

Air pollution and neonatal deaths in São Paulo, Brazil
Provedor de dados: 56
Autores: Lin,C.A.; Pereira,L.A.A.; Nishioka,D.C.; Conceição,G.M.S.; Braga,A.L.F.; Saldiva,P.H.N.
Air pollution has been associated with health effects on different age groups. The present study was designed to assess the impact of daily changes in air pollutants (NO2, SO2, CO, O3, and particle matter (PM10)) on total number of daily neonatal deaths (those that occur between the first and the 28th days of life) in São Paulo, from January 1998 to December 2000, since adverse outcomes such as neonatal deaths associated with air pollution in Brazil have not been evaluated before. Generalized additive Poisson regression models were used and nonparametric smooth functions (loess) were adopted to control long-term trend, temperature, humidity, and short-term trends. A linear term was used for holidays. The association between air pollutants and neonatal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal mortality; Infant mortality; Air pollution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500019

Follicular helper T cell in immunity and autoimmunity
Provedor de dados: 56
Autores: Mesquita Jr,D.; Cruvinel,W.M.; Resende,L.S.; Mesquita,F.V.; Silva,N.P.; Câmara,N.O.S.; Andrade,L.E.C.
The traditional concept that effector T helper (Th) responses are mediated by Th1/Th2 cell subtypes has been broadened by the recent demonstration of two new effector T helper cells, the IL-17 producing cells (Th17) and the follicular helper T cells (Tfh). These new subsets have many features in common, such as the ability to produce IL-21 and to express the IL-23 receptor (IL23R), the inducible co-stimulatory molecule ICOS, and the transcription factor c-Maf, all of them essential for expansion and establishment of the final pool of both subsets. Tfh cells differ from Th17 by their ability to home to B cell areas in secondary lymphoid tissue through interactions mediated by the chemokine receptor CXCR5 and its ligand CXCL13. These CXCR5+ CD4+ T cells are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoimmune diseases; Tfh; Lymphoid tissue; Humoral immunity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500302

Rat hindlimb joint immobilization with acrylic resin orthoses
Provedor de dados: 56
Autores: da Silva,C.A.; Guirro,R.R.J.; Polacow,M.L.O.; Cancelliero,K.M.; Durigan,J.L.Q.
The objective of the present study was to propose an orthosis of light material that would be functional for the animal and that would maintain only the ankle joint immobilized. Male Wistar rats (3 to 4 months old, 250-300 g) were divided into 2 groups (N = 6): control and immobilized for 7 days. Rats were anesthetized with sodium pentobarbital (40 mg/kg weight) and the left hindlimb was immobilized with the orthoses composed of acrylic resin model, abdominal belt and lateral supports. The following analyses were performed: glycogen content of the soleus, extensor digitorum longus, white gastrocnemius, red gastrocnemius, and tibialis anterior muscles by the phenol sulfuric method, and the weight, fiber area and intramuscular connective tissue of the soleus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Hypotrophy; Joint; Orthoses; Physical therapy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700016

Association between serotonin 2C receptor gene (HTR2C) polymorphisms and psychopathological symptoms in children and adolescents
Provedor de dados: 56
Autores: Paes,L.A.; Torre,O.H. Della; Henriques,T.B.; de Mello,M.P.; Celeri,E.H.R.V.; Dalgalarrondo,P.; Guerra Júnior,G.; dos Santos Júnior,A.
Serotonin 2C receptors (5HT2C) are involved in serotonin-driven dynamic equilibrium adjustments responsible for homeostatic stability in brain structures that modulate behavior and emotions. Single nucleotide polymorphisms (SNPs) from the serotonin 2C receptor gene (HTR2C) have been associated with several neurological and mental disorders, including abnormalities in cognitive and emotional processes. The aim of this study was to evaluate the association between the rs6318 SNP of the HTR2C gene and behavioral characteristics exhibited by children and adolescents based on the Child Behavior Checklist (CBCL/6-18) inventory. Eighty-five psychiatric outpatients between 8 and 18 years of age underwent genotyping of the rs6318 SNP. The CBCL/6-18 scale was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic polymorphism; Serotonin; CBCL/6-18; Children; Adolescents; HTR2C.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800611

Effects of chronic treadmill training on body mass gain and visceral fat accumulation in overfed rats
Provedor de dados: 56
Autores: Dantas,E.M.; Pimentel,E.B.; Gonçalves,C.P.; Lunz,W.; Rodrigues,S.L.; Mill,J.G.
This study evaluated the effects of chronic treadmill training on body mass gain and visceral fat accumulation in overfed rats. Overfeeding was induced by reducing the litter size to 3 male pups per mother during the suckling period. The litter size of control rats was adjusted to 10 male pups per mother. Seven weeks after birth overfed and normally fed rats were selected and assigned to a sedentary protocol or to a low-intensity treadmill training protocol (60 min, 5 times/week, for 9 weeks). Four groups (overfed sedentary, N = 23; normally fed sedentary, N = 32; overfed exercised, N = 18, and normally fed exercised, N = 18) were evaluated at 18 weeks. Data are reported as means ± SEM. Initial body weight was similar in control and overfed rats [8.0 ± 0.2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Exercise; Abdominal fat; Overfeeding.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500014

Alternative parameters for echocardiographic assessment of fetal diastolic function
Provedor de dados: 56
Autores: Zielinsky,P.; Nicoloso,L.H.; Firpo,C.; Marcantonio,S.; Scheid,M.; Gus,E.I.; Piccoli,A.L.; Satler,F.; Manica,J.L.; Zanettini,J.; Cardoso,R.T.
Alternative methods to assess ventricular diastolic function in the fetus are proposed. Fetal myocardial hypertrophy in maternal diabetes was used as a model of decreased left ventricular compliance (LVC), and fetal respiratory movements as a model of increased LVC. Comparison of three groups of fetuses showed that, in 10 fetuses of diabetic mothers (FDM) with septal hypertrophy (SH), the mean excursion index of the septum primum (EISP) (ratio between the linear excursion of the flap valve and the left atrial diameter) was 0.36 ± 0.09, in 8 FDM without SH it was 0.51 ± 0.09 (P = 0.001), and in the 8 normal control fetuses (NCF) it was 0.49 ± 0.12 (P = 0.003). In another study, 28 fetuses in apnea had a mean EISP of 0.39 ± 0.05 which increased to 0.57 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fetal echocardiography; Fetal diastolic function; Prenatal diagnosis; Septum primum mobility; Fetal pulmonary vein flow.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100005

Sildenafil delays the intestinal transit of a liquid meal in awake rats
Provedor de dados: 56
Autores: Graça,J.R.V; Macedo,G.M; Palheta Jr,R.C; Gondim,F. de A.A; Nogueira,R.O; Correia,J.M; Rola,F.H; Oliveira,R.B; Souza,M.A.N; Santos,A.A.
Sildenafil slows down the gastric emptying of a liquid test meal in awake rats and inhibits the contractility of intestinal tissue strips. We studied the acute effects of sildenafil on in vivo intestinal transit in rats. Fasted, male albino rats (180-220 g, N = 44) were treated (0.2 mL, iv) with sildenafil (4 mg/kg) or vehicle (0.01 N HCl). Ten minutes later they were fed a liquid test meal (99m technetium-labeled saline) injected directly into the duodenum. Twenty, 30 or 40 min after feeding, the rats were killed and transit throughout the gastrointestinal tract was evaluated by progression of the radiotracer using the geometric center method. The effect of sildenafil on mean arterial pressure (MAP) was monitored in a separate group of rats (N = 14). Data...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Intestinal transit; Mean arterial pressure; Scintigraphy; Sildenafil.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100012

Tissue transglutaminase (TG2) activity regulates osteoblast differentiation and mineralization in the SAOS-2 cell line
Provedor de dados: 56
Autores: Yin,Xiaoxue; Chen,Zhongqiang; Liu,Zhongjun; Song,Chunli.
Tissue transglutaminase (type II, TG2) has long been postulated to directly promote skeletal matrix calcification and play an important role in ossification. However, limited information is available on the expression, function and modulating mechanism of TG2 during osteoblast differentiation and mineralization. To address these issues, we cultured the well-established human osteosarcoma cell line SAOS-2 with osteo-inductive conditioned medium and set up three time points (culture days 4, 7, and 14) to represent different stages of SAOS-2 differentiation. Osteoblast markers, mineralization, as well as TG2 expression and activity, were then assayed in each stage. Furthermore, we inhibited TG activity with cystamine and then checked SAOS-2 differentiation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transglutaminase; Osteoblast; Differentiation; Mineralization; Bone morphogenetic protein-2 (BMP-2).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800002

Evaluation of the antiedematogenic activity of artemetin isolated from Cordia curassavica DC
Provedor de dados: 56
Autores: Bayeux,M.C.; Fernandes,A.T.; Foglio,M.A.; Carvalho,J.E.
The species Cordia curassavica (Boraginaceae), known popularly as "erva baleeira", is used in folk medicine for the treatment of several inflammatory processes and as a healing agent. The objective of the present study was to evaluate the antiedematogenic activity of crude dichloromethane extracts of Cordia curassavica and of the artemetin-enriched fraction. The crude extract and artemetin fraction were tested in the model of carrageenin-induced paw edema in male Swiss mice (25-30 g). The crude dichloromethane extract (300 and 1000 mg/kg, po, N = 6) showed significant antiedematogenic activity, reducing the edema by 42, 57 and 45% and 46, 62 and 69%, 3, 4 and 5 h after carrageenin administration, respectively. Indomethacin (10 mg/kg, po, N = 6) reduced the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cordia curassavica; Cordia verbenaceae; Antiedematogenic; Anti-inflammatory; Medicinal plants.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000017

Soy milk versus simvastatin for preventing atherosclerosis and left ventricle remodeling in LDL receptor knockout mice
Provedor de dados: 56
Autores: Santos,L.; Davel,A.P.; Almeida,T.I.R.; Almeida,M.R.; Soares,E.A.; Fernandes,G.J.M.; Magalhães,S.F.; Barauna,V.G.; Garcia,J.A.D.
Functional food intake has been highlighted as a strategy for the prevention of cardiovascular diseases by reducing risk factors. In this study, we compared the effects of oral treatment with soy milk and simvastatin on dyslipidemia, left ventricle remodeling and atherosclerotic lesion of LDL receptor knockout mice (LDLr-/-) fed a hyperlipidic diet. Forty 3-month old male LDLr-/- mice were distributed into four groups: control group (C), in which animals received standard diet; HL group, in which animals were fed a hyperlipidic diet; HL+SM or HL+S groups, in which animals were submitted to a hyperlipidic diet plus soy milk or simvastatin, respectively. After 60 days, both soy milk and simvastatin treatment prevented dyslipidemia, atherosclerotic lesion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soy milk Dyslipidemia Oxidative stress Inflammation Atherosclerosis; Simvastatin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300701

Viscosity of gums in vitro and their ability to reduce postprandial hyperglycemia in normal subjects
Provedor de dados: 56
Autores: Brenelli,S.L.; Campos,S.D.S.; Saad,M.J.A.
Experiments were carried out in vitro with three viscous polysaccharides (guar gum, pectin, and carboxymethylcellulose (CMC)) of similar initial viscosity submitted to conditions that mimic events occurring in the stomach and duodenum, and their viscosity in these situations was compared to their actions on postprandial hyperglycemia in normal human subjects. Guar gum showed greater viscosity than the other gums during acidification and/or alkalinization and also showed larger effects on plasma glucose levels (35% reduction in maximum rise in plasma glucose) and on the total area under the curve of plasma glucose (control: 20,314 ± 1007 mg dl-1 180 min-1 vs guar gum: 18,277 ± 699 mg dl-1 180 min-1, P&amp;lt;0.01). Pectin, which showed a marked reduction in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Viscosity of dietary fiber; Glucose tolerance test; Insulin; Guar gum; Carboxymethylcellulose; Pectin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200009

Short-term follow-up of exercise training program and beta-blocker treatment on quality of life in dogs with naturally acquired chronic mitral valve disease
Provedor de dados: 56
Autores: Marcondes-Santos,M.; Mansur,A.P.; Fragata,F.S.; Strunz,C.M.C.
This study aimed to evaluate the effects of carvedilol treatment and a regimen of supervised aerobic exercise training on quality of life and other clinical, echocardiographic, and biochemical variables in a group of client-owned dogs with chronic mitral valve disease (CMVD). Ten healthy dogs (control) and 36 CMVD dogs were studied, with the latter group divided into 3 subgroups. In addition to conventional treatment (benazepril, 0.3-0.5 mg/kg once a day, and digoxin, 0.0055 mg/kg twice daily), 13 dogs received exercise training (subgroup I; 10.3±2.1 years), 10 dogs received carvedilol (0.3 mg/kg twice daily) and exercise training (subgroup II; 10.8±1.7 years), and 13 dogs received only carvedilol (subgroup III; 10.9±2.1 years). All drugs were administered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic mitral valve disease; Beta-blocker; Exercise training; Dogs.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000004

Chronic converting enzyme inhibition normalizes QT interval in aging rats
Provedor de dados: 56
Autores: Silva,V.J. Dias da; Ferreira Neto,E.; Salgado,H.C.; Fazan Júnior,R.
The aim of the present study was to investigate the effects of converting enzyme inhibition by captopril on ECG parameters in aged rats. Four-month-old male rats received captopril dissolved in tap water (0.5 mg/l) or tap water for 2 or 20 months. At the end of treatment, under anesthesia, RR and PR interval, P wave and QRS duration, QT and corrected QT interval were measured in all animals. On the following day, chronic ECG (lead II) recordings were performed to quantify supraventricular (SVPB) or ventricular premature beats (VPB). After sacrifice, the hearts were removed and weighed. RR interval was similar in young and untreated aged rats, but significantly larger in aged rats treated with captopril. P wave and QRS length did not differ among groups. PR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Electrocardiogram; Captopril; Rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900003

Usefulness of a rapid immunometric assay for intraoperative parathyroid hormone measurements
Provedor de dados: 56
Autores: Ohe,M.N.; Santos,R.O.; Kunii,I.S.; Carvalho,A.B.; Abrahão,M.; Cervantes,O.; Lazaretti-Castro,M.; Vieira,J.G.H.
Intraoperative parathyroid hormone (IO-PTH) measurements have been proposed to improve operative success rates in primary, secondary and tertiary hyperparathyroidism (PHP, SHP and THP). Thirty-one patients requiring parathyroidectomy were evaluated retrospectively from June 2000 to January 2002. Sixteen had PHP, 7 SHP and 8 THP. Serum samples were taken at times 0 (before resection), 10, 20 and 30 min after resection of each abnormal parathyroid gland. Samples from 28 patients were frozen at -70ºC for subsequent tests, whereas samples from three patients were tested while surgery was being performed. IO-PTH was measured using the Elecsys immunochemiluminometric assay (Roche, Mannheim, Germany). The time necessary to perform the assay was 9 min. All samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parathyroid hormone; Intraoperative PTH measurement; Primary hyperparathyroidism; Secondary hyperparathyroidism; Tertiary hyperparathyroidism.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600006

Cyclosporin-A does not affect skeletal muscle mass during disuse and recovery
Provedor de dados: 56
Autores: Aoki,M.S.; Miyabara,E.H.; Soares,A.G.; Salvini,T.F.; Moriscot,A.S.
Cyclosporin-A (CsA) is an immunosuppressive drug that acts as an inhibitor of calcineurin, a calcium phosphatase that has been suggested to play a role in skeletal muscle hypertrophy. The aim of the present study was to determine the effect of CsA administration (25 mg kg-1 day-1) on skeletal muscle mass and phenotype during disuse and recovery. Male Wistar rats received vehicle (N = 8) or CsA (N = 8) during hind limb immobilization (N = 8) and recovery (N = 8). Muscle weight (dry/wet) and cross-sectional area were evaluated to verify the effect of CsA treatment on muscle mass. Muscle phenotype was assessed by histochemistry of myosin ATPase. CsA administration during immobilization and recovery did not change muscle/body weight ratio in the soleus (SOL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcineurin; Immobilization; Recovery; Skeletal muscle; Hypertrophy; Cyclosporin-A.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200011

The elevated gradient of aversion: a new apparatus to study the rat behavior dimensions of anxiety, fear, and impulsivity
Provedor de dados: 56
Autores: Rico,J.L.; Bonuti,R.; Morato,S.
Few behavioral tests allow measuring several characteristics and most require training, complex analyses, and/or are time-consuming. We present an apparatus based on rat exploratory behavior. Composed of three different environments, it allows the assessment of more than one behavioral characteristic in a short 3-min session. Factorial analyses have defined three behavioral dimensions, which we named Exploration, Impulsivity, and Self-protection. Behaviors composing the Exploration factor were increased by chlordiazepoxide and apomorphine and decreased by pentylenetetrazole. Behaviors composing the Impulsivity factor were increased by chlordiazepoxide, apomorphine, and both acute and chronic imipramine treatments. Behaviors composing the Self-protection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety; Exploratory behavior; Impulsivity; Rat; Fear; Self-protection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100601

Cardiac and renal effects induced by different exercise workloads in renovascular hypertensive rats
Provedor de dados: 56
Autores: Soares,E.R.; Lima,W.G.; Machado,R.P.; Carneiro,C.M.; Silva,M.E.; Rodrigues,M.C.; De Castro,U.G.; Santos,R.A.S.; Campagnole-Santos,M.J.; Alzamora,A.C.
We examined the effect of exercise training (Ex) without (Ex 0%) or with a 3% workload (Ex 3%) on different cardiac and renal parameters in renovascular hypertensive (2K1C) male Fisher rats weighing 150-200 g. Ex was performed for 5 weeks, 1 h/day, 5 days/week. Ex 0% or Ex 3% induced similar attenuation of baseline mean arterial pressure (MAP, 119 ± 5 mmHg in 2K1C Ex 0%, N = 6, and 118 ± 5 mmHg in 2K1C Ex 3%, N = 11, vs 99 ± 4 mmHg in sham sedentary (Sham Sed) controls, N = 10) and heart rate (HR, bpm) (383 ± 13 in 2K1C Ex 0%, N = 6, and 390 ± 14 in 2K1C Ex 3%, N = 11 vs 371 ± 11 in Sham Sed, N = 10,). Ex 0%, but not Ex 3%, improved baroreflex bradycardia (0.26 ± 0.06 ms/mmHg, N = 6, vs 0.09 ± 0.03 ms/mmHg in 2K1C Sed, N = 11). Morphometric evaluation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac and renal remodeling; Baroreflex bradycardia; Different exercise workload; Renovascular hypertension.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600011

Endogenous vasopressin and the central control of heart rate during dynamic exercise
Provedor de dados: 56
Autores: Michelini,L.C.
The present article contains a brief review on the role of vasopressinergic projections to the nucleus tractus solitarii in the genesis of reflex bradycardia and in the modulation of heart rate control during exercise. The effects of vasopressin on exercise tachycardia are discussed on the basis of both the endogenous peptide content changes and the heart rate response changes observed during running in sedentary and trained rats. Dynamic exercise caused a specific vasopressin content increase in dorsal and ventral brainstem areas. In accordance, rats pretreated with the peptide or the V1 blocker into the nucleus tractus solitarii showed a significant potentiation or a marked blunting of the exercise tachycardia, respectively, without any change in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise tachycardia; V1 receptors; Brainstem areas; Peptides; Blood pressure; Training; Rats.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900012

Food and the circadian activity of the hypothalamic-pituitary-adrenal axis
Provedor de dados: 56
Autores: Leal,A.M.O.; Moreira,A.C.
Temporal organization is an important feature of biological systems and its main function is to facilitate adaptation of the organism to the environment. The daily variation of biological variables arises from an internal time-keeping system. The major action of the environment is to synchronize the internal clock to a period of exactly 24 h. The light-dark cycle, food ingestion, barometric pressure, acoustic stimuli, scents and social cues have been mentioned as synchronizers or" zeitgebers". The circadian rhythmicity of plasma corticosteroids has been well characterized in man and in rats and evidence has been accumulated showing daily rhythmicity at every level of the hypothalamic-pituitary-adrenal (HPA) axis. Studies of restricted feeding in rats are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding; Glucocorticoids; CRH; ACTH; Circadian rhythm.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200003

Characterization of Plasmodium falciparum glutamate dehydrogenase-soluble antigen
Provedor de dados: 56
Autores: Rodríguez-Acosta,A.; Domínguez,N.G.; Aguilar,I.; Girón,M.E.
The major aim of this study was to characterize a soluble Plasmodium falciparum antigen from the plasma of malaria-infected humans and Plasmodium falciparum culture supernatants, using immunoabsorbent techniques and Western blotting. An Mr 60-kDa protein was isolated from the plasma of patients with Plasmodium falciparum malaria by affinity chromatography using rabbit anti-Proteus spp GDH(NADP+) serum as ligand. This protein, present in plasma of patients with acute Plasmodium falciparum infection, in Plasmodium falciparum culture supernatants, and in immune complexes, was tested with Plasmodium falciparum malaria hyperimmune serum from patients living in hyperendemic areas and rabbit anti-Proteus spp GDH(NADP+) serum prepared in the laboratory. In this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamate-dehydrogenase; Plasmodium falciparum; Exoantigens; Soluble antigens; Malaria; Antigenic enzymes.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900008

Association between polyclonal B cell activation and the presence of autoantibodies in mice infected with Yersinia enterocolitica O:3
Provedor de dados: 56
Eight-week old conventional female Swiss mice were inoculated intravenously with Yersinia enterocolitica O:3. A second group of normal mice was used as control. Five mice from each group were bled by heart puncture and their spleens were removed for spleen cell collection on the 3rd, 5th, 7th, 10th, 14th and 21st day after infection. Immunoglobulin-secreting spleen cells were detected by the isotype-specific protein A plaque assay. Total immunoglobulin levels were determined in mouse serum by single radial immunodiffusion and the presence of autoantibodies was determined by ELISA. We observed a marked increase in the total number of cells secreting immunoglobulins of all isotypes as early as on the 3rd day post-infection and the peak of secretion occurred...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Yersinia enterocolitica; Polyclonal activation; Experimental infection; Autoantibodies.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300015

Morphometric analysis of the phrenic nerve in male and female Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR)
Provedor de dados: 56
Autores: Rodrigues,A.R.; Ferreira,R.S.; Salgado,H.C.; Fazan,V.P.S.
Ventilatory differences between rat strains and genders have been described but the morphology of the phrenic nerve has not been investigated in spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats. A descriptive and morphometric study of the phrenic nerves of male (N = 8) and female (N = 9) SHR, and male (N = 5) and female (N = 6) WKY is presented. After arterial pressure and heart rate recordings, the phrenic nerves of 20-week-old animals were prepared for epoxy resin embedding and light microscopy. Morphometric analysis performed with the aid of computer software that took into consideration the fascicle area and diameter, as well as myelinated fiber profile and Schwann cell nucleus number per area. Phrenic nerves were generally...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phrenic nerve; Sexual dimorphism; Morphometry; Spontaneously hypertensive rats; Wistar-Kyoto rats; Asymmetry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600012

In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
Provedor de dados: 56
Autores: Ripoll-Hamer,E.; De-Paula,E.F.; Freitas,L.C.; Pereira,M.J.S.; Fonseca,L.M.; Gutfilen,B.; Carvalho,J.J.; Pôrto,L.C.M.S.; Bernardo-Filho,M.
In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioautography; Radiopharmaceuticals; Blood elements.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200014

Trans-sialidase delivered as a naked DNA vaccine elicits an immunological response similar to a Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Costa,F.; Pereira-Chioccola,V.L.; Ribeirão,M.; Schenkman,S.; Rodrigues,M.M.
Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, does not synthesize sialic acid, but expresses a trans-sialidase (TS) that catalyzes the transfer of sialic acid from host glycoconjugates to the parasite surface. Here, we review studies that characterize the immune response to the catalytic domain of the enzyme in humans during Chagas' disease or in mice following immunization with the TS gene. In both cases, there are antibodies that strongly inhibit the enzymatic activity and generation of interferon-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;-producing T cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccination; Trans-sialidase; Chagas' disease.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200013

Efficacy of a quadruple therapy regimen for Helicobacter pylori eradication after partial gastrectomy
Provedor de dados: 56
Autores: Zhang,F.; Bao,Z.J.; Shi,D.M.; Xiang,P.; Xiao,L.; Huang,Y.Q.; Zhang,G.S.; Yin,S.M.
We aimed to evaluate the effectiveness and safety of bismuth-containing quadruple therapy plus postural change after dosing for Helicobacter pylori eradication in gastrectomized patients. We compared 76 gastric stump patients with H. pylori infection (GS group) with 50 non-gastrectomized H. pylori-positive patients who met the treatment indication (controls). The GS group was divided into GS group 1 and GS group 2. All groups were administered bismuth potassium citrate (220 mg), esomeprazole (20 mg), amoxicillin (1.0 g), and furazolidone (100 mg) twice daily for 14 days. GS group 1 maintained a left lateral horizontal position for 30 min after dosing. H. pylori was detected using rapid urease testing and histologic examination of gastric mucosa before and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Gastrectomy; Gastric stump; Eradication; Efficacy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200702

The efficacy of YKL-40 and CA125 as biomarkers for epithelial ovarian cancer
Provedor de dados: 56
Autores: Zou,L.; He,X.; Zhang,J.W.
Our objective was to estimate the efficacy of the measurement of serum YKL-40 alone or with CA125 as biomarkers for the diagnosis of epithelial ovarian cancer (EOC) using the YKL-40 ELISA kit. An experimental group of 49 ovarian cancer patients included 42 patients with EOC (53 ± 15 years, range: 19-81 years) and 7 patients (48 ± 13 years, range: 29-36 years) with borderline epithelial ovarian tumor. A control group of 88 non-malignant cases included 42 patients (43 ± 10 years, range: 26-77 years) with benign gynecological disease and 46 healthy women (45 ± 14 years, range: 30-68 years) at a teaching hospital. Both YKL-40 (220.1 ± 94.1 vs 61.6 ± 48.4 and 50.1 ± 41.2 ng/mL) and CA125 (524.9 ± 972.5 vs 13.4 ± 7.6 and 28.5 ± 29.6 U/mL) levels were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YKL-40; CA125; Ovarian cancer; Diagnosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200013

Determination of anaerobic threshold in rats using the lactate minimum test
Provedor de dados: 56
Autores: Voltarelli,F.A.; Gobatto,C.A.; Mello,M.A.R. de.
The break point of the curve of blood lactate vs exercise load has been called anaerobic threshold (AT) and is considered to be an important indicator of endurance exercise capacity in human subjects. There are few studies of AT determination in animals. We describe a protocol for AT determination by the "lactate minimum test" in rats during swimming exercise. The test is based on the premise that during an incremental exercise test, and after a bout of maximal exercise, blood lactate decreases to a minimum and then increases again. This minimum value indicates the intensity of the AT. Adult male (90 days) Wistar rats adapted to swimming for 2 weeks were used. The initial state of lactic acidosis was obtained by making the animals jump into the water and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rat; Blood lactate; Lactate minimum test; Maximal lactate steady state.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100018

Combined effect of short stature and socioeconomic status on body mass index and weight gain during reproductive age in Brazilian women
Provedor de dados: 56
Autores: Sichieri,R.; Silva,C.V.C.; Moura,A.S.
Short stature, a marker for undernutrition early in life, has been associated with obesity in Brazilian women, but not in men. We tested the hypothesis that weight gain during the reproductive years could explain this gender difference. A national two-stage household survey of mothers with one or more children under five years of age was conducted in Brazil in 1996. The subjects were women aged 20 to 45 years (N = 2297), with last delivery seven months or more prior to the interview. The regions of the country were divided into rural, North/Northeast (urban underdeveloped) and South/Southeast/Midwest (urban developed). The dependent variables were current body mass index (BMI) measured, BMI prior to childbearing (reported), and BMI change. Socioeconomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parity; Body mass index; Weight gain; Pregnancy; Overweight; Stature.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000007

Retinoic acid protects from experimental cerebral infarction by upregulating GAP-43 expression
Provedor de dados: 56
Autores: Li,Y.; Gao,X.; Wang,Q.; Yang,Y.; Liu,H.; Zhang,B.; Li,L.
The aim of this study was to investigate whether exogenous retinoic acid (RA) can upregulate the mRNA and protein expression of growth-associated protein 43 (GAP-43), thereby promoting brain functional recovery in a rat distal middle cerebral artery occlusion (MCAO) model of ischemia. A total of 216 male Sprague Dawley rats weighing 300–320 g were divided into 3 groups: sham-operated group, MCAO+vehicle group and MCAO+RA group. Focal cortical infarction was induced with a distal MCAO model. The expression of GAP-43 mRNA and protein in the ipsilateral perifocal region was assessed using qPCR and immunocytochemistry at 1, 3, 7, 14, 21, and 28 days after distal MCAO. In addition, an intraperitoneal injection of RA was given 12 h before MCAO and continued...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Focal cerebral infarction; Growth associate protein 43; Retinoic acid; Nerve regeneration.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400606

Neoadjuvant chemotherapy with trastuzumab in HER2-positive breast cancer: pathologic complete response rate, predictive and prognostic factors
Provedor de dados: 56
Autores: Buzatto,I.P.C.; Ribeiro-Silva,A.; Andrade,J.M.; Carrara,H.H.A.; Silveira,W.A.; Tiezzi,D.G.
The purpose of this study was to retrospectively review the pathologic complete response (pCR) rate from patients (n=86) with stage II and III HER2-positive breast cancer treated with neoadjuvant chemotherapy at our institution from 2008 to 2013 and to determine possible predictive and prognostic factors. Immunohistochemistry for hormone receptors and Ki-67 was carried out. Clinical and pathological features were analyzed as predictive factors of response to therapy. For survival analysis, we used Kaplan-Meier curves to estimate 5-year survival rates and the log-rank test to compare the curves. The addition of trastuzumab to neoadjuvant chemotherapy significantly improved pCR rate from 4.8 to 46.8%, regardless of the number of preoperative trastuzumab...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neoadjuvant chemotherapy; Trastuzumab; Pathologic complete response; Predictive factors of response; Prognosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200702

Hepatitis C virus infection among Brazilian hemophiliacs: a virological, clinical and epidemiological study
Provedor de dados: 56
Autores: Carmo,R.A.; Oliveira,G.C.; Guimarães,M.D.C.; Oliveira,M.S.; Lima,A.A.; Buzek,S.C.; Corrêa-Oliveira,R.; Rocha,M.O.C.
We determined and analyzed risk factors of hepatitis C virus (HCV)-infected Brazilian hemophiliacs according to their virological, clinical and epidemiological characteristics. A cross-sectional and retrospective study of 469 hemophiliacs was carried out at a Brazilian blood center starting in October 1997. The prevalence of HCV infection, HCV genotypes and factors associated with HCV RNA detection was determined. The seroprevalence of anti-HCV antibodies (ELISA-3.0) was 44.6% (209/469). Virological, clinical and epidemiological assessments were completed for 162 positive patients. There were seven (4.3%) anti-HCV seroconversions between October 1992 and October 1997. During the same period, 40.8% of the positive anti-HCV hemophiliacs had abnormal alanine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; HCV genotypes; Hemophilia; Chronic liver disease; Transfusion; Brazil.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500012

Mechanisms of cell transformation induced by polyomavirus
Provedor de dados: 56
Autores: Oliveira,M.L.S.; Brochado,S.M.; Sogayar,M.C.
Polyomavirus is a DNA tumor virus that induces a variety of tumors in mice. Its genome encodes three proteins, namely large T (LT), middle T (MT), and small T (ST) antigens, that have been implicated in cell transformation and tumorigenesis. LT is associated with cell immortalization, whereas MT plays an essential role in cell transformation by binding to and activating several cytoplasmic proteins that participate in growth factor-induced mitogenic signal transduction to the nucleus. The use of different MT mutants has led to the identification of MT-binding proteins as well as analysis of their importance during cell transformation. Studying the molecular mechanisms of cell transformation by MT has contributed to a better understanding of cell cycle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Signal transduction; Malignant transformation; Polyomavirus T antigens; Polyomavirus-induced transcriptional control.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700010

Hormonal response during a fenfluramine-associated panic attack
Provedor de dados: 56
Secretion curves for prolactin, cortisol, TSH, and GH from a 37-year old woman with dysthymia and panic disorder with agoraphobia were determined one day prior to (day I), and during a panic attack (day II) associated with an oral dose of 60 mg dl-fenfluramine, a drug known to increase anticipatory anxiety. The increased cortisol secretion observed is discussed in relation to the hormonal correlates of anxiety and the possible role of depression, dl-fenfluramine, and serotonergic receptor sensitivity
Palavras-chave: Panic; Fenfluramine; Hormone secretion; Serotonin; Cortisol.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700011

CYP2A6 and CYP2E1 polymorphisms in a Brazilian population living in Rio de Janeiro
Provedor de dados: 56
Autores: Rossini,A.; Soares Lima,S.; Rapozo,D.C.M.; Faria,M.; Albano,R.M.; Ribeiro Pinto,L.F.
Cytochrome P450 (CYP) is a superfamily of enzymes involved in the metabolism of endogenous compounds and xenobiotics. CYP2A6 catalyzes the oxidation of nicotine and the activation of carcinogens such as aflatoxin B1 and nitrosamines. CYP2E1 metabolizes ethanol and other low-molecular weight compounds and can also activate nitrosamines. The CYP2A6 and CYP2E1 genes are polymorphic, altering their catalytic activities and susceptibility to cancer and other diseases. A number of polymorphisms described are ethnic-dependent. In the present study, we determined the genotype and allele frequencies of the main CYP2A6 and CYP2E1 polymorphisms in a group of 289 volunteers recruited at the Central Laboratory of Hospital Universitário Pedro Ernesto. They had been...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP2A6; CYP2E1; Polymorphism; Brazil; Ethnic groups.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200005

Changes in the TBARs content and superoxide dismutase, catalase and glutathione peroxidase activities in the lymphoid organs and skeletal muscles of adrenodemedullated rats
Provedor de dados: 56
Autores: Pereira,B.; Costa-Rosa,L.F.B.P.; Bechara,E.J.H.; Newsholme,P.; Curi,R.
Thiobarbituric acid reactant substances (TBARs) content, and the activities of glucose-6-phosphate dehydrogenase (G6PDh), citrate synthase (CS), Cu/Zn- and Mn-superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX) were measured in the lymphoid organs (thymus, spleen, and mesenteric lymph nodes (MLN)) and skeletal muscles (gastrocnemius and soleus) of adrenodemedullated (ADM) rats. The results were compared with those obtained for sham-operated rats. TBARs content was reduced by adrenodemedullation in the lymphoid organs (MLN (28%), thymus (40%) and spleen (42%)) and gastrocnemius muscle (67%). G6PDh activity was enhanced in the MLN (69%) and reduced in the spleen (28%) and soleus muscle (75%). CS activity was reduced in all tissues (MLN...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epinephrine; Lymphoid organs; Superoxide dismutase; Catalase; Glutathione peroxidase; TBARs.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600016

Autonomic abnormalities demonstrable in young normotensive subjects who are children of hypertensive parents
Provedor de dados: 56
Autores: Lopes,H.F.; Silva,H.B.; Consolim-Colombo,F.M.; Barreto Filho,J.A.S.; Riccio,G.M.G.; Giorgi,D.M.A.; Krieger,E.M.
Although a slightly elevated office blood pressure (BP) has been reported in several studies, little is known about the prolonged resting blood pressure, heart rate (HR) and baroreflex sensitivity (BRS) of prehypertensive subjects with a family history of hypertension. Office blood pressure, prolonged resting (1 h) BP and HR were measured in 25 young normotensives with a positive family history of hypertension (FH+) and 25 young normotensives with a negative family history of hypertension (FH-), matched for age, sex, and body mass index. After BP and HR measurements, blood samples were collected for the determination of norepinephrine, plasma renin activity and aldosterone levels, and baroreflex sensitivity was then tested. Casual BP, prolonged resting BP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart rate; Blood pressure; Baroreflex sensitivity; Family history of hypertension.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100007

Segmental resection combined with anoplasty for the treatment of circumferential mixed hemorrhoids
Provedor de dados: 56
Autores: Wu,Jing; Yu,Keqiang; Lv,Changyao; Lu,Wenzhu; He,Hongbo.
Circumferential mixed hemorrhoids are very difficult to treat non-surgically. Therefore, it is important to explore the surgical methods for its complete resolution as well as maintenance of normal anal anatomy and function. The present study was designed to evaluate the effect of segmented and plastic hemorrhoidectomy (SPH) on patients with circumferential mixed hemorrhoids. A total of 300 patients with circumferential mixed hemorrhoids were divided into experimental group (n=150) undergoing SPH and control group (n=150) undergoing Milligan-Morgan hemorrhoidectomy. There were no differences in cure and effectiveness rates between two groups. Compared with the control group, patients in the experimental group had shorter healing time (15.7±1.3 vs 12.5±0.7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circumferential mixed hemorrhoids; Segmented and plastic hemorrhoidectomy; Anal function; Anal smoothness; Complications.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500610

The immunomodulator glatiramer acetate influences spinal motoneuron plasticity during the course of multiple sclerosis in an animal model
Provedor de dados: 56
Autores: Marques,K.B.; Scorisa,J.M.; Zanon,R.; Freria,C.M.; Santos,L.M.B.; Damasceno,B.P.; Oliveira,A.L.R.
The immunomodulador glatiramer acetate (GA) has been shown to significantly reduce the severity of symptoms during the course of multiple sclerosis and in its animal model - experimental autoimmune encephalomyelitis (EAE). Since GA may influence the response of non-neuronal cells in the spinal cord, it is possible that, to some extent, this drug affects the synaptic changes induced during the exacerbation of EAE. In the present study, we investigated whether GA has a positive influence on the loss of inputs to the motoneurons during the course of EAE in rats. Lewis rats were subjected to EAE associated with GA or placebo treatment. The animals were sacrificed after 15 days of treatment and the spinal cords processed for immunohistochemical analysis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental autoimmune encephalomyelitis; Spinal cord; Synapse elimination; Motoneuron; Immunomodulator; Glatiramer acetate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200006

The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA
Provedor de dados: 56
Autores: Barbosa,G.H.T.S.; Santana,E.M.; Almeida,A.M.P.; Araujo,A.M.; Fatibello-Filho,O.; Carvalho Jr.,L.B.
F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filter paper; Polyvinyl alcohol; Glutaraldehyde; ELISA; Dot-ELISA; Plague.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013

Sexual behavior and fertility of male rats submitted to prolonged immobilization-induced stress
Provedor de dados: 56
Autores: Almeida,S.A.; Kempinas,W.G.; Lamano Carvalho,T.L.
In order to investigate whether prolonged stress interferes with the onset of sexual behavior at puberty and with fertility at adulthood, prepubertal male Wistar rats (40 days of age) were immobilized 6 h a day for 15 days (up to early puberty) or for 60 days (until sexual maturity). Pubertal stressed rats showed a two-fold increase in the latency for the first mount (probably due to repeated aversive experience in which a change of environment was always followed by immobilization) and a 2.5-fold increase in the frequency of thrusting (indicative of enhanced sexual performance). The apparently stimulatory effect of prolonged stress on the onset of sexual behavior is discussed in terms of increased testosterone level and interference with the complex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization stress; Sexual behavior; Male fertility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900019

Circulating high mobility group box-1 and toll-like receptor 4 expressions increase the risk and severity of epilepsy
Provedor de dados: 56
Autores: Kan,Minchen; Song,Lihong; Zhang,Xueqiang; Zhang,Jing; Fang,Pingping.
This study aimed to investigate the association of serum high-mobility group box-1 (HMGB1) and toll-like receptor 4 (TLR4) expressions with the risk of epilepsy as well as their correlations with disease severity and resistance to anti-epilepsy drugs. One hundred and five epilepsy patients and 100 healthy controls (HCs) were enrolled in this case-control study, and serum samples were collected from all participants to assess the HMGB1 and TLR4 expressions by enzyme-linked immunosorbent assay (ELISA). Both serum HMGB1 (P&lt;0.001) and TLR4 (P&lt;0.001) expressions were higher in epilepsy patients than in HCs, and they displayed good predictive values for risk of epilepsy. Moreover, HMGB1 was positively correlated with TLR4 level (r=0.735, P&lt;0.001). HMGB1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-mobility group box-1 (HMGB1); Toll-like receptor 4 (TLR4); Epilepsy; Severity; Drug resistance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700603

Propofol attenuates sepsis-induced acute kidney injury by regulating miR-290-5p/CCL-2 signaling pathway
Provedor de dados: 56
Autores: Zheng,Guodong; Qu,Hong; Li,Fen; Ma,Weiquan; Yang,Hong.
Previous studies have indicated that propofol has immunomodulatory and antioxidative properties. However, the renoprotection effect and the precise mechanisms of propofol in sepsis-induced renal injury remain unclear. The purpose of the present study was to investigate the role of miR-290-5p/CCL-2 signaling in septic mice treatment with propofol. Mice were treated with propofol (50 mg/kg) twice within 24 h. Survival outcome was monitored within 48 h. The mRNA and protein levels were assayed by qRT-PCR and western blotting, respectively. Mouse podocytes (MPC5) were treated with lipopolysaccharide (LPS) to establish the cell model in vitro. The proliferation of MPC5 was monitored using the MTS assay. Cell apoptosis was analyzed by flow cytometry. Propofol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; Sepsis; MiR-290-5p; CCL-2; Acute kidney injury.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100612

Intravenous dipyrone for the acute treatment of episodic tension-type headache: A randomized, placebo-controlled, double-blind study
Provedor de dados: 56
Autores: Bigal,M.E.; Bordini,C.A.; Speciali,J.G.
Acute headaches are responsible for a significant percentage of the case load at primary care units and emergency rooms in Brazil. Dipyrone (metamizol) is easily available in these settings, being the most frequently used drug. We conducted a randomized, placebo-controlled, double-blind study to assess the effect of dipyrone in the acute treatment of episodic tension-type headache. Sixty patients were randomized to receive placebo (intravenous injection of 10 ml saline) or 1 g dipyrone in 10 ml saline. We used seven parameters of analgesic evaluation. The patients receiving dipyrone showed a statistically significant improvement (P&lt;0.05) of pain compared to placebo up to 30 min after drug administration. The therapeutic gain was 30% in 30 min and 40% in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Episodic tension-type headache; Acute treatment; Dipyrone; Metamizol; Placebo.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000005

Cardioprotection against experimental myocardial ischemic injury using cornin
Provedor de dados: 56
Autores: Xu,Y.; Xu,Y.; Luan,H.; Jiang,Y.; Tian,X.; Zhang,S.
Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro. Effects of cornin on cell viability, as well as expression of phospho-CREB and phospho-Akt in hypoxic H9c2 cells in vitro, and myocardial I/R injury in vivo, were investigated. Cornin attenuated hypoxia-induced cytotoxicity significantly in H9c2 cells in a concentration-dependent manner. Treatment of H9c2 cells with cornin (10 µM) blocked the reduction of expression of phospho-CREB...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornin; Myocardial ischemia and reperfusion; CREB; Akt; Hypoxia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200608

Biophysical characteristics of gap junctions in vascular wall cells: implications for vascular biology and disease
Provedor de dados: 56
Autores: Brink,P.R.; Ricotta,J.; Christ,G.J.
The role gap junction channels play in the normal and abnormal functioning of the vascular wall is the subject of much research. The biophysical properties of gap junctions are an essential component in understanding how gap junctions function to allow coordinated relaxation and contraction of vascular smooth muscle. This study reviews the properties thus far elucidated and relates those properties to tissue function. We ask how biophysical and structural properties such as gating, permselectivity, subconductive states and channel type (heteromeric vs homotypic vs heterotypic) might affect vascular smooth muscle tone.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vascular smooth muscle; Gap junctions.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400007

Proliferation and osteogenic activity of fibroblasts induced with fibronectin
Provedor de dados: 56
Autores: Zhang,W.-H.; Li,X.-L.; Guo,Y.; Zhang,Y.
The aim of this study was to determine the proliferation and osteogenic activity of fibroblasts induced with fibronectin and their possible dose-dependent relationship. The fibroblasts obtained by tissue explants adherent method were induced with fibronectin at different concentrations of 0, 10, 20, 40, 60, and 80 μg/mL for 14 days. The 3H-thymidine and 3H-proline incorporation test was used to evaluate the synthesis of DNA and collagen by fibroblasts, respectively. The mineralized nodules and osteocalcin secretion, as vital osteogenic indicators, were detected with tetracycline labeling and 125I-labeled competitive immunoassay, respectively. Fibronectin significantly increased the synthesis of DNA and collagen by fibroblasts, especially at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast; Fibronectin; Proliferation; Osteogenesis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000604

Immune response to respiratory syncytial virus in young Brazilian children
Provedor de dados: 56
Autores: Queiróz,D.A.O.; Durigon,E.L.; Botosso,V.F.; Ejzemberg,B.; Vieira,S.E.; Mineo,J.R.; Yamashita,C.; Hein,N.; Lopes,C.L.; Cacharo,A.L.; Stewien,K.E.
We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients &lt;=12 months of age (39 males and 26 females, 28 cases &lt;3 months and 37 cases  &gt; or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children &lt;3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children &gt;3 months of age, with predominance of B lymphocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Respiratory syncytial virus; Peripheral blood mononuclear cells; Young children; Surface antigens; Cytokines.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011

Morphine infusions into the rostrolateral periaqueductal gray affect maternal behaviors
Provedor de dados: 56
Autores: Moura,L.M.; Canteras,N.S.; Sukikara,M.H.; Felicio,L.F.
It is well established that morphine inhibits maternal behaviors. Previous studies by our group have shown activation of the rostrolateral periaqueductal gray (rlPAG) upon inhibition-intended subcutaneous injections of morphine. In this context, we demonstrated that a single naloxone infusion into the rlPAG, following this opioid-induced inhibition, reactivated maternal behaviors. Since these data were obtained by using peripheral morphine injections, the present study was designed to test whether morphine injected directly into the rlPAG would affect maternal behaviors. Our hypothesis that morphine acting through the rlPAG would disrupt maternal behaviors was confirmed with a local infusion of morphine. The mothers showed shorter latency for locomotor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PAG; Maternal behaviors; Lactation; Opiate; Active behavior.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900013

Laboratory animal: biological reagent or living being?
Provedor de dados: 56
Autores: Cardoso,C.V.P.; Almeida,A.E.C.C. de.
The duties of humans toward non-human animals and their rights in society have been debated for a long time. However, a discussion on the terminology used for the identification of laboratory animals is usually not considered, although the employment of inadequate terminology may generate disastrous consequences for the animals before, during, and after the experiment. This study intends to defend the use of appropriate terminology, call attention to an unethical attitude of certain professionals when dealing with experimental animals, and also propose operational mechanisms, which allow for those distortions to be corrected.
Palavras-chave: Ethics; Laboratory animal; Terminology; Animal experimentation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100019

Wrist ultrasound analysis of patients with early rheumatoid arthritis
Provedor de dados: 56
Autores: Mendonça,J.A.; Yazbek,M.A.; Laurindo,I.M.M.; Bertolo,M.B.
In the present study, we evaluated 42 wrists using the semi-quantitative scales power Doppler ultrasound (PDUS) and gray scale ultrasound (GSUS) with scores ranging from 0 to 3 and correlated the results with clinical, laboratory and radiographic data. Twenty-one patients (17 women and 4 men) with rheumatoid arthritis according to criteria of the American College of Rheumatology were enrolled in the study from September 2008 to July 2009 at Universidade Estadual de Campinas (UNICAMP). The average disease duration was 14 months. The patients were 66.6% Caucasians and 33.3% non-Caucasians, with a mean age of 42 and 41 years, respectively. A dorsal longitudinal scan was performed by ultrasound on the radiocarpal and midcarpal joints using GE LOGIQ XP-linear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Power Doppler ultrasound; Gray scale ultrasound; Rheumatoid arthritis; Wrist; Synovitis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100002

Assessment of tidal volume and thoracoabdominal motion using volume and flow-oriented incentive spirometers in healthy subjects
Provedor de dados: 56
Autores: Parreira,V.F.; Tomich,G.M.; Britto,R.R.; Sampaio,R.F.
The objective of the present study was to evaluate incentive spirometers using volume- (Coach and Voldyne) and flow-oriented (Triflo II and Respirex) devices. Sixteen healthy subjects, 24 ± 4 years, 62 ± 12 kg, were studied. Respiratory variables were obtained by respiratory inductive plethysmography, with subjects in a semi-reclined position (45º). Tidal volume, respiratory frequency, minute ventilation, inspiratory duty cycle, mean inspiratory flow, and thoracoabdominal motion were measured. Statistical analysis was performed with Kolmogorov-Smirnov test, t-test and ANOVA. Comparison between the Coach and Voldyne devices showed that larger values of tidal volume (1035 ± 268 vs 947 ± 268 ml, P = 0.02) and minute ventilation (9.07 ± 3.61 vs 7.49 ± 2.58...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Incentive spirometry; Respiratory inductive plethysmography; Physiotherapy; Volume-oriented spirometer; Flow-oriented spirometer.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700014

Effect of angiotensin-(1-7) on reperfusion arrhythmias in isolated rat hearts
Provedor de dados: 56
There is increasing evidence that angiotensin-(1-7) (Ang-(1-7)) is an endogenous biologically active component of the renin-angiotensin system (RAS). In the present study, we investigated the effects of Ang-(1-7) on reperfusion arrhythmias in isolated rat hearts. Isolated rat hearts were perfused with two different media, i.e., Krebs-Ringer (2.52 mM CaCl2) and low-Ca2+ Krebs-Ringer (1.12 mM CaCl2). In hearts perfused with Krebs-Ringer, Ang-(1-7) produced a concentration-dependent (27-210 nM) reduction in coronary flow (25% reduction at highest concentration), while only slight and variable changes in contraction force and heart rate were observed. Under the same conditions, angiotensin II (Ang II; 27 and 70 nM) produced a significant reduction in coronary...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Renin-angiotensin system; Reperfusion arrhythmias; Angiotensin-(1-7); Coronary flow; Isolated heart.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600016

Artesunate promotes Th1 differentiation from CD4+ T cells to enhance cell apoptosis in ovarian cancer via miR-142
Provedor de dados: 56
Autores: Chen,Xiao; Zhang,Xue-ling; Zhang,Guo-hua; Gao,Ying-fang.
The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4+ T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4+ T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4+ T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-γ level was examined through enzyme-linked immunosorbent assay. Artesunate promoted miR-142 expression in peripheral CD4+ T cells and Th1 differentiation from CD4+ T cells. Artesunate promoted cell apoptosis of ovarian cancer cells by inducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artesunate; Ovarian cancer; CD4+ T cells; Th1 cells; MiR-142; Sirt1.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500606

There is no difference in hepatic fibrosis rates of patients infected with hepatitis C virus and those co-infected with HIV
Provedor de dados: 56
Autores: Souza,A.R.; Tovo,C.V.; Mattos,A.A.; Chaves,S.
Some studies have suggested that human immunodeficiency virus (HIV) infection modifies the natural history of hepatitis C virus (HCV) infection, accelerating the progression of fibrosis and the development of cirrhosis. Our objective was to evaluate the fibrosis progression rate (FPR) in HCV/HIV-co-infected patients, and to identify factors that may influence it. HCV-mono-infected and HCV/HIV-co-infected patients with a known date of HCV infection (transfusion or injection drug use) and a liver biopsy were included. The FPR was defined as the ratio between the fibrosis stage (Metavir score) and the estimated length of infection in years and the result was reported as fibrosis units per year. The factors studied were gender, age at infection, consumption of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Human immunodeficiency virus; Hepatic fibrosis; Cirrhosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300008

Profile of thyroid hormones in breast cancer patients
Provedor de dados: 56
Autores: Saraiva,P.P.; Figueiredo,N.B.; Padovani,C.R.; Brentani,M.M.; Nogueira,C.R.
Estrogen involvement in breast cancer has been established; however, the association between breast cancer and thyroid diseases is controversial. Estrogen-like effects of thyroid hormone on breast cancer cell growth in culture have been reported. The objective of the present study was to determine the profile of thyroid hormones in breast cancer patients. Serum aliquots from 26 patients with breast cancer ranging in age from 30 to 85 years and age-matched normal controls (N = 22) were analyzed for free triiodothyronine (T3F), free thyroxine (T4F), thyroid-stimulating hormone (TSH), antiperoxidase antibody (TPO), and estradiol (E2). Estrogen receptor ß (ERß) was determined in tumor tissues by immunohistochemistry. Thyroid disease incidence was higher in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid hormone; Estrogen receptor; Breast cancer; Hyperthyroidism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500014

The response of social anxiety disorder patients to threat scenarios differs from that of healthy controls
Provedor de dados: 56
Autores: Mesquita,S.C.V.; Shuhama,R.; Osório,F.L.; Crippa,J.A.S.; Loureiro,S.R.; Landeira-Fernandez,J.; Graeff,F.G.; Del-Ben,C.M.
The objective of the present study was to evaluate the response of social anxiety disorder (SAD) patients to threat scenarios. First-choice responses to 12 scenarios describing conspecific threatening situations and mean scores of defensive direction and defensive intensity dimensions were compared between 87 SAD patients free of medication and 87 matched healthy controls (HC). A significant gender difference in the first-choice responses was identified for seven scenarios among HCs but only for two scenarios among SAD patients. A significantly higher proportion of SAD patients chose "freezing" in response to "Bush" and "Noise" scenarios, whereas the most frequent response by HCs to these scenarios was "check out". SAD males chose "run away" and "yell"...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Social anxiety disorder; Defensive response; Threat scenarios.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200010

Routine post-weaning handling of rats prevents isolation rearing-induced deficit in prepulse inhibition
Provedor de dados: 56
Autores: Rosa,M.L.N.M.; Silva,R.C.B.; Moura-de-Carvalho,F.T.; Brandão,M.L.; Guimarães,F.S.; Del Bel,E.A.
Rats reared under isolation conditions from weaning present a number of behavioral changes compared to animals reared under social conditions (group housing). These changes include deficits in prepulse inhibition (PPI) of the startle reflex to a loud sound. PPI refers to the reduction of the magnitude of the startle reflex when a relatively weak stimulus (the prepulse) precedes by an appropriate time interval the intense startle-elicing stimulus (the pulse). PPI is useful for studying sensorimotor integration. The present study evaluated the effect of handling on the impairment of PPI induced by isolation-rearing. Male Wistar rats (N = 11-15/group) were housed in groups (5 per cage and handled three times a week) or isolated (housed individually) since...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Stress; Rat; Schizophrenia; Social isolation; Prepulse inhibition; Locomotor activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100018

Interleukin-6 and adhesion molecules VCAM-1 and ICAM-1 as biomarkers of post-acute myocardial infarction heart failure
Provedor de dados: 56
Autores: Lino,D.O.C.; Freitas,I.A.; Meneses,G.C.; Martins,A.M.C.; Daher,E.F.; Rocha,J.H.C.; Silva Junior,G.B.
Acute coronary syndromes are associated with a high prevalence of complications including heart failure (HF). The aim of this study was to investigate the association of novel biomarkers with the occurrence of post-acute myocardial infarction (AMI) HF. A prospective study was conducted with patients admitted to the emergency department with ST-segment elevation myocardial infarction (STEMI). Blood and urine samples were collected for analysis of traditional and novel biomarkers, including interleukin-6, vascular cell adhesion molecule 1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1). We compared the levels of these biomarkers between patients with and without post-STEMI HF. A total of 48 patients were assessed, with a prevalence of males. Fifteen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Acute myocardial infarction; Biomarkers; Prognosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200601

Structural heart disease as the cause of syncope
Provedor de dados: 56
Autores: Guimarães,R.B.; Essebag,V.; Furlanetto,M.; Yanez,J.P.G.; Farina,M.G.; Garcia,D.; Almeida,E.D.; Stephan,L.; Lima,G.G.; Leiria,T.L.L.
We described the clinical evolution of patients with structural heart disease presenting at the emergency room with syncope. Patients were stratified according to their syncope etiology and available scores for syncope prognostication. Cox proportional hazard models were used to investigate the relationship between etiology of the syncope and event-free survival. Of the 82,678 emergency visits during the study period, 160 (0.16%) patients were there due to syncope, having a previous diagnosis of structural heart disease. During the median follow-up of 33.8±13.8 months, mean age at the qualifying syncope event was 68.3 years and 40.6% of patients were male. Syncope was vasovagal in 32%, cardiogenic in 57%, orthostatic hypotension in 6%, and of unknown...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Syncope; Hospitalization; Structural heart; Emergency.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400616

Effect of chronic ethanol consumption in female rats subjected to experimental sepsis
Provedor de dados: 56
Autores: Castro,C.L.; Aguiar-Nemer,A.S.; Castro-Faria-Neto,H.C.; Barros,F.R.; Rocha,E.M.S.; Silva-Fonseca,V.A.
The objective of this research was to evaluate the interference of ethanol consumption by female rats with cytokines involved in the sepsis process and its correlation with mortality, the main outcome of sepsis. Female Wistar rats in estrus phase were evaluated in three experiments. Experiment 1 (n=40) was performed to determine survival rates. Experiment 2 (n=69) was designed for biochemical analysis, measurement of cytokine and estrogen levels before and after sepsis, and experiment 3 (n=10) was performed to evaluate bacterial growth by colony counts of peritoneal fluid. In all experiments, treated animals were exposed to a 10% ethanol/water solution (v/v) as the single drinking source, while untreated animals were given tap water. After 4 weeks, sepsis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcohol; Sepsis; Cytokines; Estrogens.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201033

Correction Braz J Med Biol Res 2007; 40(4): 583-590 Reduced plasma levels of angiotensin-(1-7) and renin activity in preeclamptic patients are associated with the angiotensin I-converting enzyme deletion/deletion genotype
Provedor de dados: 56
Autores: Velloso,E.P.; Vieira,R.; Cabral,A.C.; Kalapothakis,E.; Santos,R.A.S.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600020

Evidence for the participation of nitric oxide in pemphigus
Provedor de dados: 56
Autores: Siebra,M.X.; Santos,M.A.; Almeida,T.L.P.; Leite,A.C.R.M.; Cunha,F.Q.; Rocha,F.A.C.
Pemphigus is an inflammatory autoimmune disorder of the skin. Nitric oxide (NO) is an inflammatory mediator linked to a variety of physiological and pathophysiological phenomena that include skin tumors, psoriasis, urticaria, and atopic dermatitis. Inflammatory cells present in pemphigus lesions are important sources of NO production. We investigated whether NO is involved in pemphigus. A prospective cohort study was conducted at the Dermatology Service of the Hospital Universitário Walter Cantídio of the Federal University of Ceará. All patients seen at the outpatient clinic between August 2000 and July 2001, with a clinically and histologically confirmed diagnosis of pemphigus were included. The median age was 42.5 years (range: 12-69 years) with a male...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Autoimmunity; Inflammation; Neutrophils; Nitric oxide; Pemphigus; Inducible nitric oxide synthase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500014

Effect of salbutamol on innervated and denervated rat soleus muscle
Provedor de dados: 56
Autores: Šoic-Vranic,T.; Bobinac,D.; Bajek,S.; Jerkovic,R.; Malnar-Dragojevic,D.; Nikolic,M.
The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a ß2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salbutamol; Rat skeletal muscle; Soleus muscle; Denervation; Histochemistry; Muscle fibers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200008

Depressive symptoms and C-reactive protein in a Brazilian urban community
Provedor de dados: 56
Autores: Dressler,W.W.; Balieiro,M.C.; Ribeiro,R.P.; Dos-Santos,J.E.
Psychological depression is an independent risk factor for coronary artery disease. C-reactive protein has been implicated as a mediator of the effect of psychological depression. Several studies have found that individuals, especially men, who report higher levels of psychological depression also have higher levels of C-reactive protein. The current study was undertaken to replicate these results in a Brazilian population, in which there is a much wider range of variation in both background characteristics (such as socioeconomic status) and coronary artery disease risk factors. A sample of 271 individuals was interviewed using the Center for Epidemiological Studies Depression Scale. Fasting blood samples were obtained and evaluated for C-reactive protein...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: C-reactive protein; Depressive symptoms; Brazil; Gender differences.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800003

Electron paramagnetic resonance study of lipid and protein membrane components of erythrocytes oxidized with hydrogen peroxide
Provedor de dados: 56
Autores: Mendanha,S.A.; Anjos,J.L.V.; Silva,A.H.M.; Alonso,A.
Electron paramagnetic resonance (EPR) spectroscopy of spin labels was used to monitor membrane dynamic changes in erythrocytes subjected to oxidative stress with hydrogen peroxide (H2O2). The lipid spin label, 5-doxyl stearic acid, responded to dramatic reductions in membrane fluidity, which was correlated with increases in the protein content of the membrane. Membrane rigidity, associated with the binding of hemoglobin (Hb) to the erythrocyte membrane, was also indicated by a spin-labeled maleimide, 5-MSL, covalently bound to the sulfhydryl groups of membrane proteins. At 2% hematocrit, these alterations in membrane occurred at very low concentrations of H2O2 (50 µM) after only 5 min of incubation at 37°C in azide phosphate buffer, pH 7.4. Lipid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electron paramagnetic resonance; Spin label; Erythrocyte; Membrane fluidity; Membrane oxidation; Protein oxidation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600001

The Na+/glucose cotransporters: from genes to therapy
Provedor de dados: 56
Autores: Sabino-Silva,R.; Mori,R.C.; David-Silva,A.; Okamoto,M.M.; Freitas,H.S.; Machado,U.F.
Glucose enters eukaryotic cells via two types of membrane-associated carrier proteins, the Na+/glucose cotransporters (SGLT) and the facilitative glucose transporters (GLUT). The SGLT family consists of six members. Among them, the SGLT1 and SGLT2 proteins, encoded by the solute carrier genes SLC5A1 and SLC5A2, respectively, are believed to be the most important ones and have been extensively explored in studies focusing on glucose fluxes under both physiological and pathological conditions. This review considers the regulation of the expression of the SGLT promoted by protein kinases and transcription factors, as well as the alterations determined by diets of different compositions and by pathologies such as diabetes. It also considers congenital defects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SGLT1; SGLT2; SLC5A1; SLC5A2; HNF-1; Diabetes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100002

Matching salt intake to physiological need in rats using foraging protocols
Provedor de dados: 56
Autores: Rowland,N.E.
Several studies of the quantitative relationship between sodium need and sodium intake in rats are reviewed. Using acute diuretic treatment 24 h beforehand, intake matches need fairly accurately when intake is spread out in time by using a hypotonic solution of NaCl. In contrast, using a hypertonic solution, intake is typically double the need. Using the same diuretic treatment, although the natriuresis occurs within ~1 h, the appetite appears only slowly over 24 h. Increased plasma levels of aldosterone parallel the increased intake; however, treatment with metyrapone blocks the rise in aldosterone but has no effect on appetite. Satiation of sodium appetite was studied in rats using sodium loss induced by chronic diuretic treatment and daily salt...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Furosemide; Sodium depletion; Progressive ratio; Aldosterone; Satiation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500016

The E3 ligase MuRF2 plays a key role in the functional capacity of skeletal muscle fibroblasts
Provedor de dados: 56
Autores: Silvestre,J.G.; Baptista,I.L.; Silva,W.J.; Cruz,A.; Silva,M.T.; Miyabara,E.H.; Labeit,S.; Moriscot,A.S.
Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting traction forces in the extracellular matrix, which is crucial for matrix deposition and wound healing. In addition, previous studies performed on primary myoblasts suggest that the E3 ligase MuRF2 might function as a cytoskeleton adaptor. Here, we hypothesized that MuRF2 also plays a functional role in skeletal muscle fibroblasts. We found that skeletal muscle fibroblasts express MuRF2 and its siRNA knock-down promoted decreased fibroblast migration, cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast; Skeletal muscle; Migration; MuRF2; E3 ligase; Wound healing.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900604

Oral administration of L-arginine decreases blood pressure and increases renal excretion of sodium and water in renovascular hypertensive rats
Provedor de dados: 56
Autores: Gouvêa,S.A.; Moysés,M.R.; Bissoli,N.S.; Pires,J.G.P.; Cabral,A.M.; Abreu,G.R.
The two-kidney, one-clip renovascular (2K1C) hypertension model is characterized by a reduction in renal flow on the clipped artery that activates the renin-angiotensin system. Endothelium dysfunction, including diminished nitric oxide production, is also believed to play a role in the pathophysiology of this model. Some studies have shown an effect of L-arginine (L-Arg, a nitric oxide precursor) on hypertension. In the present study we determined the ability of L-Arg (7 days of treatment) to reduce blood pressure and alter renal excretions of water, Na+ and K+ in a model of 2K1C-induced hypertension. Under ether anesthesia, male Wistar rats (150-170 g) had a silver clip (0.20 mm) placed around the left renal artery to produce the 2K1C renovascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-arginine; Nitric oxide; Renovascular hypertension; Sodium excretion; Renal physiology; Blood pressure.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700017

Changes in protein profile during coagulation of latex from Carica papaya
Provedor de dados: 56
We describe the changes in peptide composition by SDS-PAGE analysis of latex from Carica papaya collected at various times after incision of the unripe fruit. The data show that during latex coagulation several peptides are processed in an orderly fashion.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Carica papaya; Latex; Endopeptidase; Cysteine proteinase; Papain; Coagulation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500007

Lipid peroxidation, antioxidant enzymes and glutathione levels in human erythrocytes exposed to colloidal iron hydroxide in vitro
Provedor de dados: 56
Autores: Ferreira,A.L.A.; Machado,P.E.A.; Matsubara,L.S.
The free form of the iron ion is one of the strongest oxidizing agents in the cellular environment. The effect of iron at different concentrations (0, 1, 5, 10, 50, and 100 µM Fe3+) on the normal human red blood cell (RBC) antioxidant system was evaluated in vitro by measuring total (GSH) and oxidized (GSSG) glutathione levels, and superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and reductase (GSH-Rd) activities. Membrane lipid peroxidation was assessed by measuring thiobarbituric acid reactive substance (TBARS). The RBC were incubated with colloidal iron hydroxide and phosphate-buffered saline, pH 7.45, at 37oC, for 60 min. For each assay, the results for the control group were: a) GSH = 3.52 ± 0.27 µM/g Hb; b) GSSG = 0.17 ± 0.03...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Iron; Erythrocyte; Thiobarbituric acid reactive substance.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600004

Analysis of serum microRNA expression in male workers with occupational noise-induced hearing loss
Provedor de dados: 56
Autores: Li,Y.H.; Yang,Y.; Yan,Y.T.; Xu,L.W.; Ma,H.Y.; Shao,Y.X.; Cao,C.J.; Wu,X.; Qi,M.J.; Wu,Y.Y.; Chen,R.; Hong,Y.; Tan,X.H.; Yang,L.
Occupational noise-induced hearing loss (ONIHL) is a prevalent occupational disorder that impairs auditory function in workers exposed to prolonged noise. However, serum microRNA expression in ONIHL subjects has not yet been studied. We aimed to compare the serum microRNA expression profiles in male workers of ONIHL subjects and controls. MicroRNA microarray analysis revealed that four serum microRNAs were differentially expressed between controls (n=3) and ONIHL subjects (n=3). Among these microRNAs, three were upregulated (hsa-miR-3162-5p, hsa-miR-4484, hsa-miR-1229-5p) and one was downregulated (hsa-miR-4652-3p) in the ONIHL group (fold change &gt;1.5 and Pbon value &lt;0.05). Real time quantitative PCR was conducted for validation of the microRNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA; Noise-induced hearing loss; Serum; MiR-1229-5p; MAPK1.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300601

The RecX protein interacts with the RecA protein and modulates its activity in Herbaspirillum seropedicae
Provedor de dados: 56
Autores: Galvão,C.W.; Souza,E.M.; Etto,R.M.; Pedrosa,F.O.; Chubatsu,L.S.; Yates,M.G.; Schumacher,J.; Buck,M.; Steffens,M.B.R.
DNA repair is crucial to the survival of all organisms. The bacterial RecA protein is a central component in the SOS response and in recombinational and SOS DNA repairs. The RecX protein has been characterized as a negative modulator of RecA activity in many bacteria. The recA and recX genes of Herbaspirillum seropedicae constitute a single operon, and evidence suggests that RecX participates in SOS repair. In the present study, we show that the H. seropedicae RecX protein (RecX Hs) can interact with the H. seropedicaeRecA protein (RecA Hs) and that RecA Hs possesses ATP binding, ATP hydrolyzing and DNA strand exchange activities. RecX Hs inhibited 90% of the RecA Hs DNA strand exchange activity even when present in a 50-fold lower molar concentration than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RecA; RecX; Herbaspirillum seropedicae; SOS repair.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200004

Immune and hormonal activity in adults suffering from depression
Provedor de dados: 56
Autores: Nunes,S.O.V.; Reiche,E.M.V.; Morimoto,H.K.; Matsuo,T.; Itano,E.N.; Xavier,E.C.D.; Yamashita,C.M.; Vieira,V.R.; Menoli,A.V.; Silva,S.S.; Costa,F.B.; Reiche,F.V.; Silva,F.L.V.; Kaminami,M.S.
An association between depression and altered immune and hormonal systems has been suggested by the results of many studies. In the present study we carried out immune and hormonal measurements in 40 non-medicated, ambulatory adult patients with depression determined by CID-10 criteria and compared with 34 healthy nondepressed subjects. The severity of the condition was determined with the Hamilton Depression Rating Scale. Of 40 depressed patients, 31 had very severe and 9 severe or moderate depression, 29 (72.5%) were females and 11 (27.5%) were males (2.6:1 ratio). The results revealed a significant reduction of albumin and elevation of alpha-1, alpha-2 and ß-globulins, and soluble IL-2 receptor in patients with depression compared to the values obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depression; Immunity; Psychoneuroimmunology; Cytokines; Acute phase proteins.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500011

Neutralization of the edema-forming, defibrinating and coagulant effects of Bothrops asper venom by extracts of plants used by healers in Colombia
Provedor de dados: 56
Autores: Núñez,V.; Otero,R.; Barona,J.; Saldarriaga,M.; Osorio,R.G.; Fonnegra,R.; Jiménez,S.L.; Díaz,A.; Quintana,J.C.
We determined the neutralizing activity of 12 ethanolic extracts of plants against the edema-forming, defibrinating and coagulant effects of Bothrops asper venom in Swiss Webster mice. The material used consisted of the leaves and branches of Bixa orellana (Bixaceae), Ficus nymphaeifolia (Moraceae), Struthanthus orbicularis (Loranthaceae) and Gonzalagunia panamensis (Rubiaceae); the stem barks of Brownea rosademonte (Caesalpiniaceae) and Tabebuia rosea (Bignoniaceae); the whole plant of Pleopeltis percussa (Polypodiaceae) and Trichomanes elegans (Hymenophyllaceae); rhizomes of Renealmia alpinia (Zingiberaceae), Heliconia curtispatha (Heliconiaceae) and Dracontium croatii (Araceae), and the ripe fruit of Citrus limon (Rutaceae). After preincubation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutralization; Edema; Defibrination; Coagulation; Bothrops asper venom; Plant extracts.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700005

Determination of myocardial infarction size in rats by echocardiography and tetrazolium staining: correlation, agreements, and simplifications
Provedor de dados: 56
Autores: Dos Santos,L.; Mello,A.F.S.; Antonio,E.L.; Tucci,P.J.F.
Triphenyltetrazolium chloride (TTC) staining and echocardiography (ECHO) are methods used to determine experimental myocardial infarction (MI) size, whose practical applicability should be expanded. Our objectives were to analyze the accuracy of ECHO in determining infarction size in rats during the first days following coronary occlusion and to test whether a simplified single measurement by TTC correctly indicates MI size, as determined by the average value for multiple slices. Infarction was induced in female Wistar rats by coronary artery occlusion and MI size analysis was performed after the acute (7th day) and chronic periods (after 4 weeks) by ECHO matched with TTC. ECHO and TTC showed similar values of MI size (% of left ventricle perimeter) in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myocardial infarction size; Echocardiography and triphenyltetrazolium comparison; Bland-Altman test.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300004

Effect of eccentric training on mitochondrial function and oxidative stress in the skeletal muscle of rats
Provedor de dados: 56
Autores: Silva,L.A.; Bom,K.F.; Tromm,C.B.; Rosa,G.L.; Mariano,I.; Pozzi,B.G.; Tuon,T.; Stresck,E.L.; Souza,C.T.; Pinho,R.A.
The objective of the present study was to investigate the effects of eccentric training on the activity of mitochondrial respiratory chain enzymes, oxidative stress, muscle damage, and inflammation of skeletal muscle. Eighteen male mice (CF1) weighing 30-35 g were randomly divided into 3 groups (N = 6): untrained, trained eccentric running (16°; TER), and trained running (0°) (TR), and were submitted to an 8-week training program. TER increased muscle oxidative capacity (succinate dehydrogenase and complexes I and II) in a manner similar to TR, and TER did not decrease oxidative damage (xylenol and creatine phosphate) but increased antioxidant enzyme activity (superoxide dismutase and catalase) similar to TR. Muscle damage (creatine kinase) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eccentric training; Mitochondrial enzyme activity; Oxidative stress; Muscle damage; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100014

Biological activity of Serratia marcescens cytotoxin
Provedor de dados: 56
Autores: Carbonell,G.V.; Amorim,C.R.N.; Furumura,M.T.; Darini,A.L.C.; Fonseca,B.A.L.; Yano,T.
Serratia marcescens cytotoxin was purified to homogeneity by ion-exchange chromatography on a DEAE Sepharose Fast Flow column, followed by gel filtration chromatography on a Sephadex G100 column. The molecular mass of the cytotoxin was estimated to be about 50 kDa. Some biological properties of the cytotoxin were analyzed and compared with well-characterized toxins, such as VT1, VT2 and CNF from Escherichia coli and hemolysin produced by S. marcescens. The sensitivity of the cell lines CHO, HeLa, HEp-2, Vero, BHK-21, MA 104 and J774 to the cytotoxin was determined by the cell viability assay using neutral red. CHO and HEp-2 were highly sensitive, with massive cellular death after 1 h of treatment, followed by BHK-21, HeLa, Vero and J774 cells, while MA 104...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell culture; Biological activity; Serratia marcescens; Cytotoxin; Virulence factors.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300010

Human papillomavirus detection and p16 methylation pattern in a case of esophageal papilloma
Provedor de dados: 56
Autores: Afonso,L.A.; Moysés,N.; Cavalcanti,S.M.B.
Esophageal cancer is a prevalent cancer worldwide. Some studies have reported the possible etiology of human papillomavirus (HPV) in benign and malignant papillomas of the esophagus but the conclusions are controversial. In the present study, we investigated an esophageal papilloma from a 30-year-old male patient presenting aphasia. HPV DNA was detected by generic PCR using MY09/11 primers, and restriction fragment length polymorphism revealed the presence of HPV54, usually associated with benign genital lesions. Hypermethylation of the pINK4A gene was also investigated due to its relation to malignant transformation, but no modification was detected in the host gene. Except for an incipient reflux, no risk factors such as cigarette smoking, alcohol abuse...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Human papillomavirus; Esophageal cancer; Restriction fragment length polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700013

Relation of cervical length at 22-24 weeks of gestation to demographic characteristics and obstetric history
Provedor de dados: 56
Autores: Palma-Dias,R.S.; Fonseca,M.M.; Stein,N.R.; Schmidt,A.P.; Magalhães,J.A.
Preterm delivery is the main cause of neonatal death and ultrasonographic cervical assessment has been shown to be more accurate than digital examination in recognizing a short cervix. This is a cross-sectional study, involving 1131 women at 22-24 weeks of pregnancy, designed to determine the distribution of cervical length and to examine which variables of demographic characteristics and obstetric history increase the risk of a short cervix (15 mm or less). The distribution of maternal demographic and obstetric history characteristics among patients with cervical length £15 mm was analyzed and compared to the findings for the general population. Risk ratios (RR) between subgroups were generated from this comparison. Median cervical length was 37 mm and in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical length; Preterm delivery screening; Transvaginal sonography; Perinatal medicine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500016

Insights on the epigenetic mechanisms underlying pulmonary arterial hypertension
Provedor de dados: 56
Autores: Luna,R.C.P.; de Oliveira,Y.; Lisboa,J.V.C.; Chaves,T.R.; de Araújo,T.A.M.; de Sousa,E.E.; Miranda Neto,M.; Pirola,L.; Braga,V.A.; de Brito Alves,J.L.
Pulmonary arterial hypertension (PAH), characterized by localized increased arterial blood pressure in the lungs, is a slow developing long-term disease that can be fatal. PAH is characterized by inflammation, vascular tone imbalance, pathological pulmonary vascular remodeling, and right-sided heart failure. Current treatments for PAH are palliative and development of new therapies is necessary. Recent and relevant studies have demonstrated that epigenetic processes may exert key influences on the pathogenesis of PAH and may be promising therapeutic targets in the prevention and/or cure of this condition. The aim of the present mini-review is to summarize the occurrence of epigenetic-based mechanisms in the context of PAH physiopathology, focusing on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigenetic; Pulmonary arterial hypertension; DNA Methylation; Histone acetylation; MiRNAs.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200301

Histidine-tryptophan-ketoglutarate solution decreases mortality and morbidity in high-risk patients with severe pulmonary arterial hypertension associated with complex congenital heart disease: an 11-year experience from a single institution
Provedor de dados: 56
Autores: Li,X.W.; Lin,Y.Z.; Lin,H.; Huang,J.B.; Tang,X.M.; Long,X.M.; Lu,W.J.; Wen,Z.K.; Liang,J.; Li,D.Y.; Zhao,X.F.
Cardioplegic reperfusion during a long term ischemic period interrupts cardiac surgery and also increases cellular edema due to repeated solution administration. We reviewed the clinical experiences on myocardial protection of a single perfusion with histidine-tryptophan-ketoglutarate (HTK) for high-risk patients with severe pulmonary arterial hypertension associated with complex congenital heart disease. This retrospective study included 101 high-risk patients undergoing arterial switch operation between March 2001 and July 2012. We divided the cohort into two groups: HTK group, myocardial protection was carried out with one single perfusion with HTK solution; and St group, myocardial protection with conventional St. Thomas' crystalloid cardioplegic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histidine-tryptophan-ketoglutarate; Myocardial protection; Cardiac surgery.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600703

Increased expression of tissue factor and protease-activated receptor-1 does not correlate with thrombosis in human lung adenocarcinoma
Provedor de dados: 56
Autores: de Meis,E.; Azambuja,D.; Ayres-Silva,J.P.; Zamboni,M.; Pinheiro,V.R.; Levy,R.A.; Monteiro,R.Q.
A correlation between cancer and hypercoagulability has been described for more than a century. Patients with cancer are at increased risk for thrombotic complications and the clotting initiator protein, tissue factor (TF), is possibly involved in this process. Moreover, TF may promote angiogenesis and tumor growth. In addition to TF, thrombin seems to play a relevant role in tumor biology, mainly through activation of protease-activated receptor-1 (PAR-1). In the present study, we prospectively studied 39 lung adenocarcinoma patients in relation to the tumor expression levels of TF and PAR-1 and their correlation with thrombosis outcome and survival. Immunohistochemical analysis showed TF positivity in 22 patients (56%), most of them in advanced stages...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung adenocarcinoma; Thrombosis; Tissue factor; Protease-activated receptor.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400012

Neurodevelopmental risk factors in schizophrenia
Provedor de dados: 56
Autores: Lobato,M.I.; Belmonte-de-Abreu,P.; Knijnik,D.; Teruchkin,B.; Ghisolfi,E.; Henriques,A.
The authors review environmental and neurodevelopmental risk factors for schizophrenic disorders, with emphasis on minor physical anomalies, particularly craniofacial anomalies and dermatoglyphic variations. The high prevalence of these anomalies among schizophrenic subjects supports the neurodevelopmental theory of the etiology of schizophrenia, since they suggest either genetically or epigenetically controlled faulty embryonic development of structures of ectodermal origin like brain and skin. This may disturb neurodevelopment that in turn may cause these subjects to be at increased risk for the development of schizophrenia and related disorders. The precise confirmation of this theory, at least in some cases, will provide further understanding of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schizophrenia; Neurodevelopmental disorder; Risk factors.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200002

Opioidergic orofacial antinociception induced by electroacupuncture at acupoint St36
Provedor de dados: 56
Autores: Almeida,R.T.; Perez,A.C.; Francischi,J.N.; Castro,M.S.; Duarte,I.D.G.
The participation of opioids in the antinociceptive effect of electroacupuncture was evaluated in terms of nociception produced by thermal stimuli applied to the face of male Wistar rats, weighing 180-230 g. Electrical stimulation (bipolar and asymmetric square wave with 0.5 mA intensity for 20 min) of acupoint St36, located in the anterior tibial muscle 10 mm distal to the knee joint, induced antinociception in the present model, which was maintained for 150 min. Acupoint LI4, located in the junction of the first and second metacarpal bones, did not achieve antinociception at any frequency studied (5 Hz: 1.7 ± 0.1; 30 Hz: 1.8 ± 0.1; 100 Hz: 1.7 ± 0.1 vs 1.4 ± 0.2). The antinociception obtained by stimulation of acupoint St36 was only achieved when high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acupuncture; St36 acupoint; Opioid; Orofacial antinociception.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700012

Anterograde effects of a single electroconvulsive shock on inhibitory avoidance and on cued fear conditioning
Provedor de dados: 56
Autores: Oliveira,M.G.M.; Bueno,O.F.A.; Gugliano,E.B.
A single electroconvulsive shock (ECS) or a sham ECS was administered to male 3-4-month-old Wistar rats 1, 2, and 4 h before training in an inhibitory avoidance test and in cued classical fear conditioning (measured by means of freezing time in a new environment). ECS impaired inhibitory avoidance at all times and, at 1 or 2 h before training, reduced freezing time before and after re-presentation of the ECS. These results are interpreted as a transient conditioned stimulus (CS)-induced anxiolytic or analgesic effect lasting about 2 h after a single treatment, in addition to the known amnesic effect of the stimulus. This suggests that the effect of anterograde learning impairment is demonstrated unequivocally only when the analgesic/anxiolytic effect is...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Electroconvulsive shock; Memory; Cued fear conditioning; Inhibitory avoidance; Anxiolytic effect; Analgesic effect.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800009

Two related thrombin-like enzymes present in Bothrops atrox venom
Provedor de dados: 56
Autores: Petretski,J.H.; Kanashiro,M.; Silva,C.P.; Alves,E.W.; Kipnis,T.L.
This article describes the presence of two new forms of a thrombin-like enzyme, both with apparent molecular masses of 38 kDa, in Bothrops atrox venom. Both share the ability to cleave fibrinogen into fibrin and to digest casein. Both present identical Km on the substrate BApNA. Their N-terminal amino acid sequences are identical for 26 residues, sharing 80% homology with batroxobin and flavoxobin. Two groups of monoclonal antibodies (mAbs) raised against the purified enzyme forms recognized different epitopes of the putative corresponding enzymes present in B. atrox crude venom. On Western blotting analysis of B. atrox crude venom, mAbs 5DB2C8, 5AA10 and 5CF11, but not mAbs 6CC5 and 6AD2-G5, revealed two or more protein bands ranging from 25 to 38 kDa. By...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venoms; Bothrops atrox; Enzymes; Thrombin-like enzymes; Blood coagulation system.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100005

A cost-effective melting temperature assay for the detection of single-nucleotide polymorphism in the MBL2 gene of HIV-1-infected children
Provedor de dados: 56
Autores: Arraes,L.C.; de Souza,P.R.; Bruneska,D.; Castelo Filho,A.; de Souza Cavada,B.; de Lima Filho,J.L.; Crovella,S.
We report a fast (less than 3 h) and cost-effective melting temperature assay method for the detection of single-nucleotide polymorphisms in the MBL2 gene. The protocol, which is based on the Corbett Rotor Gene real time PCR platform and SYBR Green I chemistry, yielded, in the cohorts studied, sensitive (100%) and specific (100%) PCR amplification without the use of costly fluorophore-labeled probes or post-PCR manipulation. At the end of the PCR, the dissociation protocol included a slow heating from 60º to 95ºC in 0.2ºC steps, with an 8-s interval between steps. Melting curve profiles were obtained using the dissociation software of the Rotor Gene-3000 apparatus. Samples were analyzed in duplicate and in different PCR runs to test the reproducibility of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Melting temperature assay; Real time PCR; MBL2; Polymorphisms; HIV-1-positive children.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600003

Antinociceptive properties of the essential oil of Ocimum gratissimum L. (Labiatae) in mice
Provedor de dados: 56
Autores: Rabelo,M.; Souza,E.P.; Soares,P.M.G.; Miranda,A.V.; Matos,F.J.A.; Criddle,D.N.
We have investigated the antinociceptive effects of the essential oil of Ocimum gratissimum L. (Labiatae) (EOOG) in two classical models of pain in male Swiss mice (25-35 g), the writhing test and the formalin test. At doses of 30, 100 and 300 mg/kg (po), EOOG produced a dose-dependent inhibition (from 58.3 ± 4.4 to 40.7 ± 6.3, 36.4 ± 3.6 and 24.6 ± 3.6, respectively; N = 8-10, P&lt;0.05) of acetic acid-induced writhing, causing up to a ~60% inhibition at the highest dose used, comparable to that obtained with indomethacin (10 mg/kg, po). At the same doses, EOOG predominantly inhibited the late (inflammatory) phase of the formalin-induced pain response (from 59.3 ± 8.3 to 40.4 ± 4.8, 23.2 ± 2.8 and 25.3 ± 5.5, respectively; N = 6, P&lt;0.05), with a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ocimum gratissimum L.; Labiatae; Essential oil; Antinociception.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400016

Vitamin B1 and B6 in the malaria parasite: requisite or dispensable?
Provedor de dados: 56
Autores: Wrenger,C.; Knöckel,J.; Walter,R.D.; Müller,I.B.
Vitamins are essential compounds mainly involved in acting as enzyme co-factors or in response to oxidative stress. In the last two years it became apparent that apicomplexan parasites are able to generate B vitamers such as vitamin B1 and B6 de novo. The biosynthesis pathways responsible for vitamin generation are considered as drug targets, since both provide a high degree of selectivity due to their absence in the human host. This report updates the current knowledge about vitamin B1 and B6 biosynthesis in malaria and other apicomplexan parasites. Owing to the urgent need for novel antimalarials, the significance of the biosynthesis and salvage of these vitamins is critically discussed in terms of parasite survival and their exploitation for drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Apicomplexa; Pyridoxal phosphate synthase; Vitamin B6; Vitamin B1.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200001

Category fluency test: effects of age, gender and education on total scores, clustering and switching in Brazilian Portuguese-speaking subjects
Provedor de dados: 56
Autores: Brucki,S.M.D.; Rocha,M.S.G.
Verbal fluency tests are used as a measure of executive functions and language, and can also be used to evaluate semantic memory. We analyzed the influence of education, gender and age on scores in a verbal fluency test using the animal category, and on number of categories, clustering and switching. We examined 257 healthy participants (152 females and 105 males) with a mean age of 49.42 years (SD = 15.75) and having a mean educational level of 5.58 (SD = 4.25) years. We asked them to name as many animals as they could. Analysis of variance was performed to determine the effect of demographic variables. No significant effect of gender was observed for any of the measures. However, age seemed to influence the number of category changes, as expected for a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Verbal fluency; Educational level; Neuropsychological tests; Category fluency.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200002

Expression of extracellular matrix components and their receptors in the central nervous system during experimental Toxoplasma gondii and Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Silva,A.A.; Roffê,E.; Lannes-Vieira,J.
Alterations in extracellular matrix (ECM) expression in the central nervous system (CNS) usually associated with inflammatory lesions have been described in several pathological situations including neuroblastoma and demyelinating diseases. The participation of fibronectin (FN) and its receptor, the VLA-4 molecule, in the migration of inflammatory cells into the CNS has been proposed. In Trypanosoma cruzi infection encephalitis occurs during the acute phase, whereas in Toxoplasma infection encephalitis is a chronic persisting process. In immunocompromised individuals such as AIDS patients, T. cruzi or T. gondii infection can lead to severe CNS damage. At the moment, there are no data available regarding the molecules involved in the entrance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Trypanosoma cruzi; Central nervous system; Extracellular matrix.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500013

Acute effect of amiodarone on cardiovascular reflexes of normotensive and renal hypertensive rats
Provedor de dados: 56
Autores: Oliveira,P.F.; Dias da Silva,V.J.; Salgado,M.C.O.; Fazan Jr.,R.; Aguiar,C.A.; Salgado,H.C.
The aim of the present study was to evaluate the effect of amiodarone on mean arterial pressure (MAP), heart rate (HR), baroreflex, Bezold-Jarisch, and peripheral chemoreflex in normotensive and chronic one-kidney, one-clip (1K1C) hypertensive rats (N = 9 to 11 rats in each group). Amiodarone (50 mg/kg, iv) elicited hypotension and bradycardia in normotensive (-10 ± 1 mmHg, -57 ± 6 bpm) and hypertensive rats (-37 ± 7 mmHg, -39 ± 19 bpm). The baroreflex index (deltaHR/deltaMAP) was significantly attenuated by amiodarone in both normotensive (-0.61 ± 0.12 vs -1.47 ± 0.14 bpm/mmHg for reflex bradycardia and -1.15 ± 0.19 vs -2.63 ± 0.26 bpm/mmHg for reflex tachycardia) and hypertensive rats (-0.26 ± 0.05 vs -0.72 ± 0.16 bpm/mmHg for reflex bradycardia and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amiodarone; Baroreflex; Bezold-Jarisch reflex; Chemoreflex; Autonomic nervous system; 1K1C hypertension.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600020

Contribution of matrix vesicles and alkaline phosphatase to ectopic bone formation
Provedor de dados: 56
Autores: Ciancaglini,P.; Simão,A.M.S.; Camolezi,F.L.; Millán,J.L.; Pizauro,J.M.
Endochondral calcification involves the participation of matrix vesicles (MVs), but it remains unclear whether calcification ectopically induced by implants of demineralized bone matrix also proceeds via MVs. Ectopic bone formation was induced by implanting rat demineralized diaphyseal bone matrix into the dorsal subcutaneous tissue of Wistar rats and was examined histologically and biochemically. Budding of MVs from chondrocytes was observed to serve as nucleation sites for mineralization during induced ectopic osteogenesis, presenting a diameter with Gaussian distribution with a median of 306 ± 103 nm. While the role of tissue-nonspecific alkaline phosphatase (TNAP) during mineralization involves hydrolysis of inorganic pyrophosphate (PPi), it is unclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix vesicles; Endochondral ossification; Osseous plate; Alkaline phosphatase; Ectopic mineralization; Calcification.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500006

Determination of low tetanus or diphtheria antitoxin titers in sera by a toxin neutralization assay and a modified toxin-binding inhibition test
Provedor de dados: 56
Autores: Sonobe,M.H.; Trezena,A.G.; Guilhen,F.B.; Takano,V.L.; Fratelli,F.; Sakauchi,D.; Morais,J.F.; Prado,S.M.A.; Higashi,H.G.
A method for the screening of tetanus and diphtheria antibodies in serum using anatoxin (inactivated toxin) instead of toxin was developed as an alternative to the in vivo toxin neutralization assay based on the toxin-binding inhibition test (TOBI test). In this study, the serum titers (values between 1.0 and 19.5 IU) measured by a modified TOBI test (Modi-TOBI test) and toxin neutralization assays were correlated (P &lt; 0.0001). Titers of tetanus or diphtheria antibodies were evaluated in serum samples from guinea pigs immunized with tetanus toxoid, diphtheria-tetanus or triple vaccine. For the Modi-TOBI test, after blocking the microtiter plates, standard tetanus or diphtheria antitoxin and different concentrations of guinea pig sera were incubated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetanus; Diphtheria; Toxin-binding inhibition test; Toxin neutralization.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100009

The eukaryotic Pso2/Snm1/Artemis proteins and their function as genomic and cellular caretakers
Provedor de dados: 56
Autores: Bonatto,D.; Revers,L.F.; Brendel,M.; Henriques,J.A.P.
DNA double-strand breaks (DSBs) represent a major threat to the genomic stability of eukaryotic cells. DNA repair mechanisms such as non-homologous end joining (NHEJ) are responsible for the maintenance of eukaryotic genomes. Dysfunction of one or more of the many protein complexes that function in NHEJ can lead to sensitivity to DNA damaging agents, apoptosis, genomic instability, and severe combined immunodeficiency. One protein, Pso2p, was shown to participate in the repair of DSBs induced by DNA inter-strand cross-linking (ICL) agents such as cisplatin, nitrogen mustard or photo-activated bi-functional psoralens. The molecular function of Pso2p in DNA repair is unknown, but yeast and mammalian cell line mutants for PSO2 show the same cellular responses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-homologous end joining; Double-strand breaks; V(D)J; PSO2; Artemis; Saccharomyces cerevisiae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300002

Leukotrienes are not essential for the efficacy of a heterologous vaccine against Mycobacterium tuberculosis infection
Provedor de dados: 56
Autores: Franco,L.H.; Paula,M. Oliveira e; Wowk,P.F.; Fonseca,D.M. da; Sérgio,C.A.; Fedatto,P.F.; Gembre,A.F.; Ramos,S.G.; Silva,C.L.; Medeiros,A.I.; Faccioli,L.H.; Bonato,V.L.D.
Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 µg) (boost). During the 30 days following the challenge, the animals were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tuberculosis; Prime-boost immunization; Leukotrienes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700006

Physical exercise prevents motor disorders and striatal oxidative imbalance after cerebral ischemia-reperfusion
Provedor de dados: 56
Autores: Sosa,P.M.; Schimidt,H.L.; Altermann,C.; Vieira,A.S.; Cibin,F.W.S.; Carpes,F.P.; Mello-Carpes,P.B.
Stroke is the third most common cause of death worldwide, and most stroke survivors present some functional impairment. We assessed the striatal oxidative balance and motor alterations resulting from stroke in a rat model to investigate the neuroprotective role of physical exercise. Forty male Wistar rats were assigned to 4 groups: a) control, b) ischemia, c) physical exercise, and d) physical exercise and ischemia. Physical exercise was conducted using a treadmill for 8 weeks. Ischemia-reperfusion surgery involved transient bilateral occlusion of the common carotid arteries for 30 min. Neuromotor performance (open-field and rotarod performance tests) and pain sensitivity were evaluated beginning at 24 h after the surgery. Rats were euthanized and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stroke; Striatum; Locomotion; Oxidative stress; Antioxidants; Running.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900798

Acetylcholine and bradykinin enhance hypotension and affect the function of remodeled conduit arteries in SHR and SHR treated with nitric oxide donors
Provedor de dados: 56
Autores: Gerová,M.; Kristek,F.; Cacányiová,S.; Cebová,M.
Discrepancy was found between enhanced hypotension and attenuated relaxation of conduit arteries in response to acetylcholine (ACh) and bradykinin (BK) in nitric oxide (NO)-deficient hypertension. The question is whether a similar phenomenon occurs in spontaneously hypertensive rats (SHR) with a different pathogenesis. Wistar rats, SHR, and SHR treated with NO donors [molsidomine (50 mg/kg) or pentaerythritol tetranitrate (100 mg/kg), twice a day, by gavage] were studied. After 6 weeks of treatment systolic blood pressure (BP) was increased significantly in experimental groups. Under anesthesia, the carotid artery was cannulated for BP recording and the jugular vein for drug administration. The iliac artery was used for in vitro studies and determination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Conduit iliac artery; Resistance arteries; Molsidomine; Pentaerythritol tetranitrate; Iliac artery; Structure.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600019

Rabbit model of uncontrolled hemorrhagic shock and hypotensive resuscitation
Provedor de dados: 56
Autores: Rezende-Neto,J.B.; Rizoli,S.B.; Andrade,M.V.; Lisboa,T.A.; Cunha-Melo,J.R.
Clinically relevant animal models capable of simulating traumatic hemorrhagic shock are needed. We developed a hemorrhagic shock model with male New Zealand rabbits (2200-2800 g, 60-70 days old) that simulates the pre-hospital and acute care of a penetrating trauma victim in an urban scenario using current resuscitation strategies. A laparotomy was performed to reproduce tissue trauma and an aortic injury was created using a standardized single puncture to the left side of the infrarenal aorta to induce hemorrhagic shock similar to a penetrating mechanism. A 15-min interval was used to simulate the arrival of pre-hospital care. Fluid resuscitation was then applied using two regimens: normotensive resuscitation to achieve baseline mean arterial blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemorrhagic shock; Animal model; Resuscitation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200003

Modulation of murine blastocyst hatching in vitro by glutamine and tryptophan
Provedor de dados: 56
Autores: Castro,W.; Bueno Sánchez,J.C.; Piedrahita-Ochoa,C.; Cadavid J.,A.P.
Enrichment of culture media with amino acids improves embryo development. However, little is known about the specific action of each amino acid during embryogenesis. The present study was undertaken to examine the effect of L-glutamine (Gln) and tryptophan (Trp) on mouse embryo hatching, expansion and viability in vitro. Blastocysts were collected from 6- to 8-week-old female BALB/c mice (N = 30) and cultured in M2 medium containing either 0.125, 0.25 or 0.5 mM Trp, 1 mM Gln, or M2 alone. Gln significantly increased (100%; P &lt; 0.05) blastocyst hatching at 24 h compared to M2 alone or Trp; moreover, Trp inhibited blastocyst hatching when compared to M2 alone (P &lt; 0.05) at 72 h. In contrast, the percentage of embryos reaching the state of expanded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo development; Murine blastocyst; Hatching; Glutamine; Tryptophan.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800003

Classification of different degrees of adiposity in sedentary rats
Provedor de dados: 56
Autores: Leopoldo,A.S.; Lima-Leopoldo,A.P.; Nascimento,A.F.; Luvizotto,R.A.M.; Sugizaki,M.M.; Campos,D.H.S.; da Silva,D.C.T.; Padovani,C.R.; Cicogna,A.C.
In experimental studies, several parameters, such as body weight, body mass index, adiposity index, and dual-energy X-ray absorptiometry, have commonly been used to demonstrate increased adiposity and investigate the mechanisms underlying obesity and sedentary lifestyles. However, these investigations have not classified the degree of adiposity nor defined adiposity categories for rats, such as normal, overweight, and obese. The aim of the study was to characterize the degree of adiposity in rats fed a high-fat diet using cluster analysis and to create adiposity intervals in an experimental model of obesity. Thirty-day-old male Wistar rats were fed a normal (n=41) or a high-fat (n=43) diet for 15 weeks. Obesity was defined based on the adiposity index; and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Adiposity index; Rats; Cluster analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400601

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3
Provedor de dados: 56
Autores: Liu,Z.; Zhang,P.; Zhou,Y.; Qin,H.; Shen,T.
Epithelium, a highly dynamic system, plays a key role in the homeostasis of the intestine. However, thus far a human intestinal epithelial cell line has not been established in many countries. Fetal tissue was selected to generate viable cell cultures for its sterile condition, effective generation, and differentiated character. The purpose of the present study was to culture human intestinal epithelial cells by a relatively simple method. Thermolysin was added to improve the yield of epithelial cells, while endothelin-3 was added to stimulate their growth. By adding endothelin-3, the achievement ratio (viable cell cultures/total cultures) was enhanced to 60% of a total of 10 cultures (initiated from 8 distinct fetal small intestines), allowing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human intestinal epithelial cell; Cell culture; Thermolysin; Endothelin-3; Cytokeratin; Brush border enzyme.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500006

CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals
Provedor de dados: 56
Autores: Mikawa,A.Y.; Tagliavini,S.A.; Costa,P.I.
The 32-bp deletion in the HIV-1 co-receptor CCR5 confers a high degree of resistance to HIV-1 infection in homozygous individuals for the deleted allele and partial protection against HIV-1 during disease progression in heterozygotes. Natural ligands for CCR5, MIP-1alpha, MIP-1ß and RANTES, have been shown to inhibit HIV replication in CD4+ T cells. In the present study, we examined the CCR5 genotype by PCR and the plasma levels of RANTES and MIP-1alpha by ELISA among blood donors (N = 26) and among HIV-1-infected individuals (N = 129). The control group consisted of healthy adult volunteers and HIV-1-infected subjects were an asymptomatic and heterogeneous group of individuals with regard to immunologic and virologic markers of HIV-1 disease. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV-1; CC chemokine receptor 5; Delta32ccr5 Frequency allele; Chemokines.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100011

5-HT1A autoreceptor modulation of locomotor activity induced by nitric oxide in the rat dorsal raphe nucleus
Provedor de dados: 56
Autores: Gualda,L.B.; Martins,G.G.; Müller,B.; Guimarães,F.S.; Oliveira,R.M.W.
The dorsal raphe nucleus (DRN) is the origin of ascending serotonergic projections and is considered to be an important component of the brain circuit that mediates anxiety- and depression-related behaviors. A large fraction of DRN serotonin-positive neurons contain nitric oxide (NO). Disruption of NO-mediated neurotransmission in the DRN by NO synthase inhibitors produces anxiolytic- and antidepressant-like effects in rats and also induces nonspecific interference with locomotor activity. We investigated the involvement of the 5-HT1A autoreceptor in the locomotor effects induced by NO in the DRN of male Wistar rats (280-310 g, N = 9-10 per group). The NO donor 3-morpholinosylnomine hydrochloride (SIN-1, 150, and 300 nmol) and the NO scavenger...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dorsal raphe nucleus; Nitric oxide; Serotonin; 5-HT1A receptor; Locomotor activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400010

Modeling pulmonary fibrosis by abnormal expression of telomerase/apoptosis/collagen V in experimental usual interstitial pneumonia
Provedor de dados: 56
Autores: Parra,E.R.; Pincelli,M.S.; Teodoro,W.R.; Velosa,A.P.P.; Martins,V.; Rangel,M.P.; Barbas-Filho,J.V.; Capelozzi,V.L.
Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor of apoptosis. We evaluated the quantitative relationship between the telomerase/apoptosis index, collagen V synthesis, and epithelial/fibroblast replication in mice exposed to butylated hydroxytoluene (BHT) at high oxygen concentration. Two groups of mice were analyzed: 20 mice received BHT, and 10 control mice received corn oil. Telomerase expression, apoptosis, collagen I, III, and V fibers, and hydroxyproline were evaluated by immunohistochemistry, in situ detection of apoptosis, electron microscopy, immunofluorescence, and histomorphometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BHT experimental model; Usual interstitial pneumonia; Alveolar epithelial cell apoptosis; Telomerase activity; Collagen V; Electron microscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700567

Rapid eye movement (REM) sleep deprivation reduces rat frontal cortex acetylcholinesterase (EC 3.1.1.7) activity
Provedor de dados: 56
Rapid eye movement (REM) sleep deprivation induces several behavioral changes. Among these, a decrease in yawning behavior produced by low doses of cholinergic agonists is observed which indicates a change in brain cholinergic neurotransmission after REM sleep deprivation. Acetylcholinesterase (Achase) controls acetylcholine (Ach) availability in the synaptic cleft. Therefore, altered Achase activity may lead to a change in Ach availability at the receptor level which, in turn, may result in modification of cholinergic neurotransmission. To determine if REM sleep deprivation would change the activity of Achase, male Wistar rats, 3 months old, weighing 250-300 g, were deprived of REM sleep for 96 h by the flower-pot technique (N = 12). Two additional...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: REM sleep deprivation; Frontal cortex; Acetylcholinesterase.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500012

pp-Blast: a "pseudo-parallel" Blast
Provedor de dados: 56
Autores: Osório,E.C.; Souza,J.E. de; Zaiats,A.C.; Oliveira,P.S.L. de; Souza,S.J. de.
We have developed a software called pp-Blast that uses the publicly available Blast package and PVM (parallel virtual machine) to partition a multi-sequence query across a set of nodes with replicated or shared databases. Benchmark tests show that pp-Blast running in a cluster of 14 PCs outperformed conventional Blast running in large servers. In addition, using pp-Blast and the cluster we were able to map all human cDNAs onto the draft of the human genome in less than 6 days. We propose here that the cost/benefit ratio of pp-Blast makes it appropriate for large-scale sequence analysis. The source code and configuration files for pp-Blast are available at http://www.ludwig.org.br/biocomp/tools/pp-blast.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Blast; Search engines; Fasta.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400007

Cytokine production profile of heart-infiltrating T cells in Chagas' disease cardiomyopathy
Provedor de dados: 56
Autores: Cunha-Neto,E.; Rizzo,L.V.; Albuquerque,F.; Abel,L.; Guilherme,L.; Bocchi,E.; Bacal,F.; Carrara,D.; Ianni,B.; Mady,C.; Kalil,J.
The hallmark of chronic Chagas' disease cardiomyopathy (CCC) is the finding of a T cell-rich inflammatory mononuclear cell infiltrate in the presence of extremely few parasites in the heart lesions. The scarcity of parasites in affected heart tissue casts doubt on the direct participation of Trypanosoma cruzi in CCC heart tissue lesions, and suggests the possible involvement of autoimmunity. The cells in the infiltrate are presumably the ultimate effectors of tissue damage, and there is evidence that such cells recognize cardiac myosin in molecular mimicry with T. cruzi proteins rather than primary reactivity to T. cruzi antigens (Cunha-Neto et al. (1996) Journal of Clinical Investigation, 98: 1709-1712). Recently, we have studied heart-infiltrating T...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Chagas' disease cardiomyopathy; Immunology; Cytokines; Gamma-interferon.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100018

Binding sites and actions of Tx1, a neurotoxin from the venom of the spider Phoneutria nigriventer, in guinea pig ileum
Provedor de dados: 56
Autores: Santos,R.G.; Diniz,C.R.; Cordeiro,M.N.; De Lima,M.E.
Tx1, a neurotoxin isolated from the venom of the South American spider Phoneutria nigriventer, produces tail elevation, behavioral excitation and spastic paralysis of the hind limbs after intracerebroventricular injection in mice. Since Tx1 contracts isolated guinea pig ileum, we have investigated the effect of this toxin on acetylcholine release, as well as its binding to myenteric plexus-longitudinal muscle membranes from the guinea pig ileum. [125I]-Tx1 binds specifically and with high affinity (Kd = 0.36 ± 0.02 nM) to a single, non-interacting (nH = 1.1), low capacity (Bmax 1.1 pmol/mg protein) binding site. In competition experiments using several compounds (including ion channel ligands), only PhTx2 and PhTx3 competed with [125I]-Tx1 for specific...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Phoneutria nigriventer; Spider toxins; Binding; Enteric nervous system; Calcium channels; Guinea pig ileum.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200019

Humoral response to low molecular weight antigens of Mycobacterium tuberculosis by tuberculosis patients and contacts
Provedor de dados: 56
Autores: Beck,S.T.; Leite,O.M.; Arruda,R.S.; Ferreira,A.W.
Much effort has been devoted to the identification of immunologically important antigens of Mycobacterium tuberculosis and to the combination of target antigens to which antibodies from serum of tuberculous patients could react specifically. We searched for IgG antibodies specific for antigens of 45 to 6 kDa obtained after sonication of the well-characterized wild M. tuberculosis strain in order to detect differences in the antibody response to low molecular weight antigens from M. tuberculosis between patients with pulmonary tuberculosis and contacts. Specific IgG antibodies for these antigens were detected by Western blot analysis of 153 serum samples collected from 51 patients with confirmed pulmonary tuberculosis. Three samples were collected from each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Western blot; Profile; Tuberculosis; Antigens.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400013

Potential virulence of Klebsiella sp. isolates from enteral diets
Provedor de dados: 56
Autores: Pereira,S.C.L.; Vanetti,M.C.D.
We aimed to evaluate the potential virulence of Klebsiella isolates from enteral diets in hospitals, to support nosocomial infection control measures, especially among critical-care patients. Phenotypic determination of virulence factors, such as capsular expression on the external membrane, production of aerobactin siderophore, synthesis of capsular polysaccharide, hemolytic and phospholipase activity, and resistance to antibiotics, which are used therapeutically, were investigated in strains of Klebsiella pneumoniae and K. oxytoca. Modular industrialized enteral diets (30 samples) as used in two public hospitals were analyzed, and Klebsiella isolates were obtained from six (20%) of them. The hypermucoviscous phenotype was observed in one of the K....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella; Enteral diets; Pathogenicity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900782

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle
Provedor de dados: 56
Autores: Antunes,M.M.; Godoy,G.; de Almeida-Souza,C.B.; da Rocha,B.A.; da Silva-Santi,L.G.; Masi,L.N.; Carbonera,F.; Visentainer,J.V.; Curi,R.; Bazotte,R.B.
We previously reported that both the high-carbohydrate diet (HCD) and high-fat diet (HFD) given for two months promote lipid deposition and inflammation in the liver and brain of mice. The results obtained indicate a tissue-specific response to both diets. Herein, we compared the effects of HCD and HFD on fatty acid (FA) composition and inflammation in the gastrocnemius muscle. Male Swiss mice were fed with HCD or HFD for 1 or 2 months. Saturated FA (SFA), monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA), and n-6 PUFA were quantified. The activities of stearoyl-CoA desaturase 1 (SCD-1), Δ-6 desaturase (D6D), elongase 6, and de novo lipogenesis (DNL) were estimated. As for indicators of the inflammatory tissue state, we measured myeloperoxidase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saturated fatty acids; Monounsaturated fatty acids; Polyunsaturated fatty acids; N-6/n-3 PUFA ratio; SFA/n-3 PUFA ratio.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300603

Normal range values for thromboelastography in healthy adult volunteers
Provedor de dados: 56
Autores: Scarpelini,S.; Rhind,S.G.; Nascimento,B.; Tien,H.; Shek,P.N.; Peng,H.T.; Huang,H.; Pinto,R.; Speers,V.; Reis,M.; Rizoli,S.B.
Thromboelastography (TEG®) provides a functional evaluation of coagulation. It has characteristics of an ideal coagulation test for trauma, but is not frequently used, partially due to lack of both standardized techniques and normal values. We determined normal values for our population, compared them to those of the manufacturer and evaluated the effect of gender, age, blood type, and ethnicity. The technique was standardized using citrated blood, kaolin and was performed on a Haemoscope 5000 device. Volunteers were interviewed and excluded if pregnant, on anticoagulants or having a bleeding disorder. The TEG® parameters analyzed were R, K, α, MA, LY30, and coagulation index. All volunteers outside the manufacturer’s normal range underwent extensive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulation; Thromboelastography; Normal values.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200015

The brain decade in debate: VII. Neurobiology of sleep and dreams
Provedor de dados: 56
Autores: Aloe,F.; Amzica,F.; Hening,W.; Menna-Barreto,L.; Pinto Jr.,L.R.; Velluti,R.; Vertes,R.; Timo-Iaria,C.
This article is a transcription of an electronic symposium held on February 5, 2001 by the Brazilian Society of Neuroscience and Behavior (SBNeC) during which eight specialists involved in clinical and experimental research on sleep and dreaming exposed their personal experience and theoretical points of view concerning these highly polemic subjects. Unlike most other bodily functions, sleep and dreaming cannot, so far, be defined in terms of definitive functions that play an ascribable role in maintaining the organism as a whole. Such difficulties appear quite clearly all along the discussions. In this symposium, concepts on sleep function range from a protective behavior to an essential function for maturation of the nervous system. Kleitman's hypothesis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep; Dreams; Consciousness; Rapid eye movement; REM.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200002

Endothelial mediators of 17ß-estradiol-induced coronary vasodilation in the isolated rat heart
Provedor de dados: 56
Autores: Santos,R.L.; Abreu,G.R.; Bissoli,N.S.; Moysés,M.R.
The present study was designed to determine relaxation in response to 17ß-estradiol by isolated perfused hearts from intact normotensive male and female rats as well as the contribution of endothelium and its relaxing factors to this action. Baseline coronary perfusion pressure was determined and the vasoactive effects of 17ß-estradiol (10 µM) were assessed by in bolus administration before and after endothelium denudation by infusion of 0.25 µM sodium deoxycholate or perfusion with 100 µM L-NAME, 2.8 µM indomethacin, 0.75 µM clotrimazole, 100 µM L-NAME plus 2.8 µM indomethacin, and 100 µM L-NAME plus 0.75 µM clotrimazole. Baseline coronary perfusion pressure differed significantly between males (84 ± 2 mmHg, N = 61) and females (102 ± 2 mmHg, N = 61)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogen; Vasodilation; Endothelium-derived relaxing factors; Coronary arteries.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400014

Association between the increase in brain temperature and physical performance at different exercise intensities and protocols in a temperate environment
Provedor de dados: 56
Autores: Kunstetter,A.C.; Wanner,S.P.; Madeira,L.G.; Wilke,C.F.; Rodrigues,L.O.C.; Lima,N.R.V.
There is evidence that brain temperature (Tbrain) provides a more sensitive index than other core body temperatures in determining physical performance. However, no study has addressed whether the association between performance and increases in Tbrain in a temperate environment is dependent upon exercise intensity, and this was the primary aim of the present study. Adult male Wistar rats were subjected to constant exercise at three different speeds (18, 21, and 24 m/min) until the onset of volitional fatigue. Tbrain was continuously measured by a thermistor inserted through a brain guide cannula. Exercise induced a speed-dependent increase in Tbrain, with the fastest speed associated with a higher rate of Tbrain increase. Rats subjected to constant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain cortex; Fatigue; Hyperthermia; Speed; Thermoregulation; Treadmill running.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800679

Possible benefit of consolidation therapy with high-dose cytarabine on overall survival of adults with non-promyelocytic acute myeloid leukemia
Provedor de dados: 56
Autores: Azevedo,M.C.; Velloso,E.D.R.P.; Buccheri,V.; Chamone,D.A.F.; Dorlhiac-Llacer,P.E.
In adults with non-promyelocytic acute myeloid leukemia (AML), high-dose cytarabine consolidation therapy has been shown to influence survival in selected patients, although the appropriate doses and schemes have not been defined. We evaluated survival after calculating the actual dose of cytarabine that patients received for consolidation therapy and divided them into 3 groups according to dose. We conducted a single-center, retrospective study involving 311 non-promyelocytic AML patients with a median age of 36 years (16-79 years) who received curative treatment between 1978 and 2007. The 131 patients who received cytarabine consolidation were assigned to study groups by their cytarabine dose protocol. Group 1 (n=69) received &lt;1.5 g/m2 every 12 h on 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; Chemotherapy; Consolidation therapy; High-dose cytarabine; Prognosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200178

The cognitive subscale of the "Alzheimer's Disease Assessment Scale" in a Brazilian sample
Provedor de dados: 56
Autores: Schultz,R.R.; Siviero,M.O.; Bertolucci,P.H.F.
The cognitive subscale of the "Alzheimer's Disease Assessment Scale" (ADAS-Cog) is widely used for the evaluation of dementia and is very popular in dementia drug trials because of the characteristics of the scale. The objective of the present study was to adapt the ADAS-Cog for use for the Brazilian population. A major problem is that education is variable, a fact that may influence performance in cognitive evaluation. This study was conducted on a control group (CG) of 96 subjects (25 males and 71 females aged 68 ± 8.6 years) and on 44 Alzheimer's disease (AD) patients (19 males and 25 females aged 72 ± 6.4 years) with mild dementia (Clinical Dementia Rating score 1). For statistical analysis groups were divided into three educational levels: I, 0-4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dementia; Alzheimer's disease; Neuropsychological evaluation; Education.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000009

Neutrophil function and metabolism in individuals with diabetes mellitus
Provedor de dados: 56
Autores: Alba-Loureiro,T.C.; Munhoz,C.D.; Martins,J.O.; Cerchiaro,G.A.; Scavone,C.; Curi,R.; Sannomiya,P.
Neutrophils act as first-line-of-defense cells and the reduction of their functional activity contributes to the high susceptibilityto and severity of infections in diabetes mellitus. Clinical investigations in diabetic patients and experimental studies in diabetic rats and mice clearly demonstrated consistent defects of neutrophil chemotactic, phagocytic and microbicidal activities. Other alterations that have been reported to occur during inflammation in diabetes mellitus include: decreased microvascular responses to inflammatory mediators such as histamine and bradykinin, reduced protein leakage and edema formation, reduced mast cell degranulation, impairment of neutrophil adhesionto the endothelium and migration to the site of inflammation, production...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutrophils; Inflammation; Diabetes mellitus; Insulin; Metabolism and cellular function.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800003

A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins
Provedor de dados: 56
Autores: Rocha,H.A.O.; Franco,C.R.C.; Trindade,E.S.; Carvalho,L.C.M.; Veiga,S.S.; Leite,E.L.; Dietrich,C.P.; Nader,H.B.
Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5))...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CHO cells; Extracellular matrix proteins; Fucan; Glycosaminoglycans; Cell adhesion; Brown algae.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500009

Agents that affect cAMP levels or protein kinase A activity modulate memory consolidation when injected into rat hippocampus but not amygdala
Provedor de dados: 56
Autores: Bevilaqua,L.; Ardenghi,P.; Schröder,N.; Bromberg,E.; Quevedo,J.; Schmitz,P.K.; Bianchin,M.; Walz,R.; Schaeffer,E.; Medina,J.H.; Izquierdo,I.
Male Wistar rats were trained in one-trial step-down inhibitory avoidance using a 0.4-mA footshock. At various times after training (0, 1.5, 3, 6 and 9 h for the animals implanted into the CA1 region of the hippocampus; 0 and 3 h for those implanted into the amygdala), these animals received microinfusions of SKF38393 (7.5 µg/side), SCH23390 (0.5 µg/side), norepinephrine (0.3 µg/side), timolol (0.3 µg/side), 8-OH-DPAT (2.5 µg/side), NAN-190 (2.5 µg/side), forskolin (0.5 µg/side), KT5720 (0.5 µg/side) or 8-Br-cAMP (1.25 µg/side). Rats were tested for retention 24 h after training. When given into the hippocampus 0 h post-training, norepinephrine enhanced memory whereas KT5720 was amnestic. When given 1.5 h after training, all treatments were ineffective....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory formation; Memory modulation; Hippocampus; Amygdala; CAMP/PKA/CREB-P pathway; Dopamine D; Receptors; SS-adrenoceptors; 5HT1A receptors.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800009

Cloning, expression and purification of recombinant streptokinase: partial characterization of the protein expressed in Escherichia coli
Provedor de dados: 56
Autores: Avilán,L.; Yarzábal,A.; Jürgensen,C.; Bastidas,M.; Cruz,J.; Puig,J.
We cloned the streptokinase (STK) gene of Streptococcus equisimilis in an expression vector of Escherichia coli to overexpress the profibrinolytic protein under the control of a tac promoter. Almost all the recombinant STK was exported to the periplasmic space and recovered after gentle lysozyme digestion of induced cells. The periplasmic fraction was chromatographed on DEAE Sepharose followed by chromatography on phenyl-agarose. Active proteins eluted between 4.5 and 0% ammonium sulfate, when a linear gradient was applied. Three major STK derivatives of 47.5 kDa, 45 kDa and 32 kDa were detected by Western blot analysis with a polyclonal antibody. The 32-kDa protein formed a complex with human plasminogen but did not exhibit Glu-plasminogen activator...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant streptokinase; Plasminogen activators; Protein purification.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200007

Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning
Provedor de dados: 56
Autores: Cui,S.Q.; Wang,Q.; Zheng,Y.; Xiao,B.; Sun,H.W.; Gu,X.L.; Zhang,Y.C.; Fu,C.H.; Dong,P.X.; Wang,X.M.
We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puerarin; Chronic alcohol poisoning; Neuroprotection; Microglia; Inflammatory factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600515

Response threshold to aversive stimuli in stimulated early protein-malnourished rats
Provedor de dados: 56
Two animal models of pain were used to study the effects of short-term protein malnutrition and environmental stimulation on the response threshold to aversive stimuli. Eighty male Wistar rats were used. Half of the pups were submitted to malnutrition by feeding their mothers a 6% protein diet from 0 to 21 days of age while the mothers of the other half (controls) were well nourished, receiving 16% protein. From 22 to 70 days all rats were fed commercial lab chow. Half of the animals in the malnourished and control groups were maintained under stimulating conditions, including a 3-min daily handling from 0 to 70 days and an enriched living cage after weaning. The other half was reared in a standard living cage. At 70 days, independent groups of rats were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Early protein malnutrition; Enriched environment; Shock threshold test; Tail-flick test.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300016

The future of Brazilian Biochemistry
Provedor de dados: 56
Autores: Lara,F.J.S.
Tipo: Info:eu-repo/semantics/article
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200002

Ultrastructural study of the neovagina following the utilization of human amniotic membrane for treatment of congenital absence of the vagina
Provedor de dados: 56
We present an ultrastructural study of the utilization of human amniotic membrane in the treatment of congenital absence of the vagina in 10 patients. All patients were surgically treated with application of an amniotic membrane graft using the modified McIndoe and Bannister technique. Sixty days after surgery, samples of the vaginal neo-epithelium were collected for transmission electron microscopy analysis. The ultrastructural findings consisted of a lining of mature squamous epithelium indicating the occurrence of metaplasia of the amniotic epithelium into the vaginal epithelium. The cells were arranged in layers as in the normal vaginal epithelium, i.e., superficial, intermediate and deep layers. There were desmosomes and cytoplasmic intermediate...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Amniotic membrane; Amnion; Electron microscopy; Congenital absence of vagina; Neovagina.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700007

CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
Provedor de dados: 56
Autores: Wang,X.M.; Gao,S.J.; Guo,X.F.; Sun,W.J.; Yan,Z.Q.; Wang,W.X.; Xu,Y.Q.; Lu,D.
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CIAPIN1 gene; Multidrug resistance; RNA interference; MDR1 gene; Breast neoplasms.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400273

Neuropeptides in the posterodorsal medial amygdala modulate central cardiovascular reflex responses in awake male rats
Provedor de dados: 56
Autores: Quagliotto,E.; Casali,K.R.; Dal Lago,P.; Rasia-Filho,A.A.
The rat posterodorsal medial amygdala (MePD) links emotionally charged sensory stimuli to social behavior, and is part of the supramedullary control of the cardiovascular system. We studied the effects of microinjections of neuroactive peptides markedly found in the MePD, namely oxytocin (OT, 10 ng and 25 pg; n=6/group), somatostatin (SST, 1 and 0.05 μM; n=8 and 5, respectively), and angiotensin II (Ang II, 50 pmol and 50 fmol; n=7/group), on basal cardiovascular activity and on baroreflex- and chemoreflex-mediated responses in awake adult male rats. Power spectral and symbolic analyses were applied to pulse interval and systolic arterial pressure series to identify centrally mediated sympathetic/parasympathetic components in the heart rate variability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreceptor and chemoreceptor reflexes; Power spectral analysis; Symbolic analysis; Oxytocin; Somatostatin; Angiotensin II.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200128

Correction
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/report
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400013

Frequency of Fanconi anemia in Brazil and efficacy of screening for the FANCA 3788-3790del mutation
Provedor de dados: 56
Autores: Magdalena,N.; Pilonetto,D.V.; Bitencourt,M.A.; Pereira,N.F.; Ribeiro,R.C.; Jeng,M.; Pasquini,R.
Fanconi anemia (FA) is an autosomal recessive genetic disease characterized by progressive bone marrow failure, susceptibility to cancer and multiple congenital anomalies. There is important clinical variability among patients and the knowledge of factors which might predict outcome would greatly help the decision making regarding the choices of treatment and the appropriate time to start it. Future studies of the possible correlation between specific mutations with specific clinical presentations will provide the answer to one of these factors. At our Center we standardized a rapid and precise screening test using a mismatch PCR assay for a specific mutation (3788-3790del in exon 38 of gene FANCA) in Brazilian FA patients. We present the results obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anemia; FANCA; 3788-3790del mutation; Genetic screening.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500003

Effect of (+)-dehydrofukinone on GABAA receptors and stress response in fish model
Provedor de dados: 56
Autores: Garlet,Q.I.; Pires,L.C.; Silva,D.T.; Spall,S.; Gressler,L.T.; Bürger,M.E.; Baldisserotto,B.; Heinzmann,B.M.
(+)-Dehydrofukinone (DHF) is a major component of the essential oil of Nectandra grandiflora (Lauraceae), and exerts a depressant effect on the central nervous system of fish. However, the neuronal mechanism underlying DHF action remains unknown. This study aimed to investigate the action of DHF on GABAA receptors using a silver catfish (Rhamdia quelen) model. Additionally, we investigated the effect of DHF exposure on stress-induced cortisol modulation. Chemical identification was performed using gas chromatography-mass spectrometry and purity was evaluated using gas chromatography with a flame ionization detector. To an aquarium, we applied between 2.5 and 50 mg/L DHF diluted in ethanol, in combination with 42.7 mg/L diazepam. DHF within the range of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sedation; Anesthesia; Rhamdia quelen; Nectandra grandiflora; Cortisol.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100607

Reliability and validity of heart rate variability threshold assessment during an incremental shuttle-walk test in middle-aged and older adults
Provedor de dados: 56
Autores: Dourado,V.Z.; Guerra,R.L.F.
Studies on the assessment of heart rate variability threshold (HRVT) during walking are scarce. We determined the reliability and validity of HRVT assessment during the incremental shuttle walk test (ISWT) in healthy subjects. Thirty-one participants aged 57 ± 9 years (17 females) performed 3 ISWTs. During the 1st and 2nd ISWTs, instantaneous heart rate variability was calculated every 30 s and HRVT was measured. Walking velocity at HRVT in these tests (WV-HRVT1 and WV-HRVT2) was registered. During the 3rd ISWT, physiological responses were assessed. The ventilatory equivalents were used to determine ventilatory threshold (VT) and the WV at VT (WV-VT) was recorded. The difference between WV-HRVT1 and WV-HRVT2 was not statistically significant (median and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart rate variability; Exercise; Walking; Adults.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200194

Effect of sildenafil (Viagra®) on the genital reflexes of paradoxical sleep-deprived male rats
Provedor de dados: 56
Autores: Andersen,M.L.; Bignotto,M.; Tufik,S.
Since there is evidence that paradoxical sleep deprivation (PSD) elicits penile erection (PE) and ejaculation (EJ), and that the erectile response of rats is mediated by nitric oxide, the present study sought to extend the latter finding by assessing the effects of sildenafil on the genital reflexes of male Wistar rats subjected to PSD. We also determined the influence of sildenafil on hormone concentrations. In the first experiment, sildenafil at doses ranging from 0.08 to 0.32 mg/kg was administered intraperitoneally to rats that had been deprived of sleep for 4 days and to home cage controls (N = 8-10/group). The frequency of PE and EJ was measured for 60 min. PSD alone induced PE in 50% of the animals; however, a single injection of sildenafil did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paradoxical sleep deprivation; Sildenafil; Genital reflexes; Dopamine; Testosterone; Progesterone.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100008

Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells
Provedor de dados: 56
Autores: Xiao,J.B.; Chen,X.Q.; Zhang,Y.W.; Jiang,X.Y.; Xu,M.
The cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol) from a plant used in folk medicine, Marchantia convoluta, to human non-small cell lung carcinoma (H1299) and liver carcinoma (HepG2) cell lines was tested. After 72-h incubation of lung and liver cancer cell cultures with varying concentrations of extracts (15 to 200 µg/mL), cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and reported in terms of cell viability. The extracts that showed a significant cytotoxicity were subjected to gas chromatography-mass spectrometry analysis to identify the components. The ethyl acetate, but not the petroleum ether or n-butanol extract, had a significant cytotoxicity against lung and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marchantia convoluta extract; Cytotoxicity assay; HepG2 and H1299 carcinoma cell lines; Phytol; Caryophyllene oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600005

In situ enzyme immunoassay for titration of a Brazilian hepatitis A virus strain (HAF-203)
Provedor de dados: 56
Autores: Villar,L.M.; Amado,L.A.; Gaspar,A.M.C.
Hepatitis A virus (HAV) replicates relatively slowly in cell culture without a cytopathic effect, a fact that limits the use of tissue culture assays. The radioimmunofocus assay is the standard method for HAV titration, although it is labor intensive and requires the use of radioisotopes. A simple, rapid and objective infectivity assay based on an in situ enzyme immunoassay (EIA) is described here for a Brazilian cell culture-adapted HAV strain (HAF-203). The assay uses a peroxidase-labeled polyclonal antibody to fixed monolayers as an indicator of infection. EIA may be completed within 7 days using serial 5-fold dilutions of the virus, yielding a titer of 5.024 log 50% tissue culture infective dose (TCID50)/ml for HAF-203. This technique had a detection...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis A virus; Virus titration; Enzyme immunoassay.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700011

Prevalence of celiac disease in Brazilian children of short stature
Provedor de dados: 56
Autores: Queiroz,M.S.; Nery,M.; Cançado,E.L.; Gianella-Neto,D.; Liberman,B.
The aim of the present study was to determine the prevalence of celiac disease in children of short stature and to assess whether some of the routine laboratory examinations performed to determine the cause of short stature could suggest the presence of celiac disease. A total of 106 children of short stature and no gastrointestinal symptoms were studied. An extensive endocrine work-up had been negative for all of them and an additional investigation was performed by measuring the concentration of antiendomysial antibody. Patients who were positive for antiendomysial antibody ( &gt; or = 1:10) or who exhibited IgA deficiency (less than 5 mg/dl) were referred for an endoscopic intestinal biopsy. We detected a pathological titer of antiendomysial IgA in six...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celiac disease; Short stature; Antiendomysial antibody; Growth failure; Celiac sprue.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100008

Behavioral characteristics of the offspring of adolescent rats
Provedor de dados: 56
Autores: Zemunik,T.; Peruzovic,M.; Capkun,V.; Milkovic,K.
The aim of this study was to test the hypothesis that, during adulthood, the offspring of adolescent rats differ in emotionality, learning and memory from the offspring of adult rats. The behavior of the offspring of adolescent (age, 50-55 days) and adult rats (age, 90-95 days) was tested in the open field, activity cage, and passive and active avoidance apparatus. The latencies during training and testing in the passive avoidance apparatus of the offspring of adolescent parents were shorter than the latencies of control offspring (P&lt;0.001 on both training and testing days). Offspring of adolescent parents showed shorter latency time in acquisition trials during active avoidance testing compared to control offspring (P&lt;0.001). They also showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adolescence; Pregnancy; Behavior; Learning and memory; Emotionality.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400008

Distribution of AMPA-type glutamate receptor subunits in the chick visual system
Provedor de dados: 56
Several glutamate receptor (GluR) subunits have been characterized during the past few years. In the present study, subunit-specific antisera were used to determine the distribution of the AMPA-type glutamate receptor subunits GluR1-4 in retinorecipient areas of the chick brain. Six white leghorn chicks (Gallus gallus, 7-15 days old, unknown sex) were deeply anesthetized and perfused with 4% buffered paraformaldehyde and brain sections were stained using immunoperoxidase techniques. The AMPA-type glutamate receptor subunits GluR1, GluR2/3 and GluR4 were present in several retinorecipient areas, with varying degrees of colocalization. For example, perikarya in layers 2, 3, and 5 of the optic tectum contained GluR1, whereas GluR2/3 subunits appeared mainly...
Palavras-chave: Glutamate; Receptors; Neurotransmitters; Visual system; Visual pathways.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100012

Role of Leishmania (Leishmania) amazonensis amastigote glycosphingolipids in macrophage infectivity
Provedor de dados: 56
Autores: Tanaka,A.K.; Gorin,P.A.J.; Takahashi,H.K.; Straus,A.H.
The role of glycosphingolipids (GSLs) present in amastigote forms of Leishmania (Leishmania) amazonensis during infection of macrophages was analyzed, with particular emphasis on GSLs presenting the terminal Galpß1-3Galpa disaccharide. Macrophage invasion by L. (L.) amazonensis amastigotes was reduced by 37% when the disaccharide Galpß1-3Galp (1 mM) was added to the culture medium. The putative macrophage receptor/lectin for ß-Gal-globotriaosylceramide (Galpß1-3Galpa1-4Galpß1-4Glc pß1-1Cer) and other structurally related GSLs from L. (L.) amazonensis amastigotes were analyzed by micelles and parasite binding assay to peritoneal macrophage proteins fractionated by SDS-PAGE under nonreducing conditions. Micelles containing purified amastigote GSLs or a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania (Leishmania) amazonensis; Amastigote; Glycosphingolipid; Macrophage binding protein.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600008

Interhemispheric connections between primary visual areas: beyond the midline rule
Provedor de dados: 56
Autores: Houzel,J.-C.; Carvalho,M.L.; Lent,R.
In the last five years, a number of detailed anatomical, electrophysiological, optical imaging and simulation studies performed in a variety of non-human species have revealed that the functional organization of callosal connections between primary visual areas is more elaborate than previously thought. Callosal cell bodies and terminals are clustered in columns whose correspondence to features mapped in the visual cortex, such as orientation and ocularity, are starting to be understood. Callosal connections are not restricted to the vertical midline representation nor do they establish merely point-to-point retinotopic correspondences across the hemispheres, as traditionally believed. In addition, anatomical studies have revealed the existence of an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corpus callosum; Visual cortex; Perceptual binding; Vertical midline; Orientation column; Binocular interactions.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001200005

Functions of the extracellular matrix and matrix degrading proteases during tumor progression
Provedor de dados: 56
Autores: Liaw,L.; Crawford,H.C.
Cell interactions with extracellular matrices are important to pathological changes that occur during cell transformation and tumorigenesis. Several extracellular matrix proteins including fibronectin, thrombospondin-1, laminin, SPARC, and osteopontin have been suggested to modulate tumor phenotype by affecting cell migration, survival, or angiogenesis. Likewise, proteases including the matrix metalloproteinases (MMPs) are understood to not only facilitate migration of cells by degradation of matrices, but also to affect tumor formation and growth. We have recently demonstrated an in vivo role for the RGD-containing protein, osteopontin, during tumor progression, and found evidence for distinct functions in the host versus the tumor cells. Because of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix metalloproteinase; Osteopontin; Carcinoma; Protease.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700002

Oxygen consumption of rats with broad intestinal resection
Provedor de dados: 56
Autores: Luz,J.; Griggio,M.A.; Fagundes,D.J.; Araújo,R.M.; Marcondes,W.
The study was performed to investigate possible alterations in oxygen consumption in an animal model with broad intestinal resection. Oxygen consumption and the thermal effect of a short meal were measured in rats subjected to short bowel syndrome. Four groups of rats were used. Group I was the control group, group II was sham operated, group III was submitted to 80% jejunum-ileum resection, and group IV was submitted to 80% jejunum-ileum resection with colon interposition. Ninety days after surgery, oxygen consumption was measured over a period of 6 h with the animals fasted overnight. The thermal effect of feeding was determined in another session of oxygen consumption measurement in animals fasted for 12 h. A 12-kcal meal was then introduced into the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cephalic thermal effect of food; Energy metabolism; Intestinal resection; Oxygen consumption; Short bowel syndrome.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200015

Electro-oscillographic correlation between dorsal raphe nucleus, neocortex and hippocampus during wakefulness before and after serotoninergic inactivation
Provedor de dados: 56
Autores: Fonoff,E.T.; Silva,C.P.C.; Ballester,G.; Timo-Iaria,C.
Theta rhythm in many brain structures characterizes wakefulness and desynchronized sleep in most subprimate mammalian brains. In close relation to behaviors, theta frequency and voltage undergo a fine modulation which may involve mobilization of dorsal raphe nucleus efferent pathways. In the present study we analyzed frequency modulation (through instantaneous frequency variation) of theta waves occurring in three cortical areas, in hippocampal CA1 and in the dorsal raphe nucleus of Wistar rats during normal wakefulness and after injection of the 5-HT1a receptor agonist 8-OH-DPAT into the dorsal raphe. We demonstrated that in attentive states the variation of theta frequency among the above structures is highly congruent, whereas after 8-OH-DPAT injection,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Theta rhythm; Serotonin; Dorsal raphe nucleus; Neocortex; Hippocampus; Instantaneous frequency.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400015

Effect of actinomycin D on simian rotavirus (SA11) replication in cell culture
Provedor de dados: 56
Autores: Stefanelli,C.C.; Castilho,J.G.; Botelho,M.V.J.; Linhares,R.E.C.; Nozawa,C.M.
Rotaviruses are the major cause of viral diarrhea in humans and animals. Actinomycin D (Act D) is an antibiotic that intercalates DNA and therefore inhibits DNA-dependent transcription. The current study was carried out to assess the influence of Act D on the replication of simian rotavirus (SA11) in cell culture. Virus-infected MA-104 cell cultures were studied in the presence of Act D at concentrations of 1.25 and 2.5 µg/ml. Treatment of rotavirus-infected cells with 2.5 µg/ml Act D 48 h post-infection reduced the cytoplasmic metachromasia after staining with acridine orange by 25%. Viral RNA labeled with ³H-uridine in the presence of the drug was separated by polyacrylamide gel electrophoresis. Viral RNA replication was not affected by Act D, but...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rotavirus; Actinomycin D; Replication; Cell culture.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400006

Cardiovascular responses to locomotor activity and feeding in unrestrained three-toed sloths, Bradypus variegatus
Provedor de dados: 56
Autores: Duarte,D.P.F.; Jaguaribe,A.M.; Pedrosa,M.A.C.; Clementino,A.C.C.R.; Barbosa,A.A.; Silva,A.F.V.; Gilmore,D.P.; Da Costa,C.P.
Heart rate (HR) and systolic (SBP), diastolic (DBP) and mean (MBP) blood pressure were recorded by biotelemetry in nine conscious unrestrained sloths for 1 min every 15 min over a 24-h period. The animals were allowed to freely move in an acoustically isolated and temperature-controlled (24 ± 1ºC) experimental room with light-dark cycle (12/12 h). Behavior was closely monitored through a unidirectional visor and classified as resting (sitting or suspended), feeding (chewing and swallowing embauba leaves, Cecropia adenops), or locomotor activity around the tree trunk or on the room floor. Locomotor activity caused statistically significant increases in SBP (+8%, from 121 ± 22 to 131 ± 18 mmHg), DBP (+7%, from 86 ± 17 to 92 ± 10 mmHg), MBP (+8%, from 97 ± 19...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tree-toed sloth; Heart rate; Blood pressure; Locomotor activity; Feeding behavior.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000016

Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood
Provedor de dados: 56
Autores: Gomes,N.E.; Brunialti,M.K.C.; Mendes,M.E.; Freudenberg,M.; Galanos,C.; Salomão,R.
Lipopolysaccharide (LPS) activates neutrophils and monocytes, inducing a wide array of biological activities. LPS rough (R) and smooth (S) forms signal through Toll-like receptor 4 (TLR4), but differ in their requirement for CD14. Since the R-form LPS can interact with TLR4 independent of CD14 and the differential expression of CD14 on neutrophils and monocytes, we used the S-form LPS from Salmonella abortus equi and the R-form LPS from Salmonella minnesota mutants to evaluate LPS-induced activation of human neutrophils and monocytes in whole blood from healthy volunteers. Expression of cell surface receptors and reactive oxygen species (ROS) and nitric oxide (NO) generation were measured by flow cytometry in whole blood monocytes and neutrophils. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipopolysaccharide; Neutrophils; Monocytes; Cell surface receptors; Reactive oxygen species; Nitric oxide.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900007

CD47 expression is decreased in hematopoietic progenitor cells in patients with myelofibrosis
Provedor de dados: 56
Autores: Nonino,A.; Nascimento,J.M.; Mascarenhas,C.C.; Mazzeu,J.F.; Pereira,R.W.; Jacomo,R.H.
Myelofibrosis (MF) is characterized by increased circulating hematopoietic progenitor cells (HPCs), abnormal cytokine levels, and the survival advantage of neoplastic progenitors over their normal counterparts, which leads to progressive disappearance of polyclonal hematopoiesis. CD47 is a surface glycoprotein with many functions, such as acting as a phagocytosis inhibitor of the expressing cell, that is increased in normal hematopoietic stem and progenitor cells mobilized into the blood and several human cancer-initiating cells, such as in acute myeloid leukemia. We compared CD47 expression in hematopoietic stem and progenitor cells of patients with MF and controls and found it to be decreased in progenitors of MF. Exposure of control HPCs to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myeloproliferative disorders; Primary myelofibrosis; Hematopoietic stem cells; Neoplastic stem cells; Antigens; CD47.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100610

Alcohol dependence induced in rats by semivoluntary intermittent intake
Provedor de dados: 56
Autores: Macieira,M.S.; Almeida,W.G.; Silva,E.A.; Schenberg,L.C.; Nakamura-Palacios,E.M.
The objective of the present experiment was to assess ethyl alcohol (ETOH) dependence brought about by a semivoluntary intermittent intake regimen in rats. Male Wistar rats weighing 150-250 g at the onset of the experiment were assigned to the following groups: 0% ETOH (N = 11), 5% ETOH (N = 20), 20% ETOH (N = 20) and 40% ETOH (N = 18). ETOH solutions were offered at the end of the day and overnight from Monday to Friday, and throughout weekends, for 90 days. The concentration of the ETOH solutions was increased in a stepwise fashion allowing the rats to get used to the taste of alcohol. Reposition of pure water was permitted during 1-h water drinking periods in the morning. Daily volume intake (± SEM) averaged 25.4 ± 0.4 ml (0% ETOH), 23.8 ± 0.6 ml (5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Alcohol; Drug dependence; Rat.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900009

Ventilation-induced changes correlate to pulmonary vascular response and VEGF, VEGFR-1/2, and eNOS expression in the rat model of postnatal hypoxia
Provedor de dados: 56
Autores: Figueira,R.L.; Gonçalves,F.L.; Prado,A.R.; Ribeiro,M.C.; Costa,K.M.; Silva,O. Castro e; Sbragia,L.
Neonatal asphyxia occurs due to reduction in oxygen supply to vital organs in the newborn. Rapid restoration of oxygen to the lungs after a long period of asphyxia can cause lung injury and decline of respiratory function, which result from the activity of molecules that induce vascular changes in the lung such as nitric oxide (NO) and vascular endothelial growth factors (VEGF). In this study, we evaluated the pulmonary and vascular morphometry of rats submitted to the model of neonatal asphyxia and mechanical ventilation, their expression of pulmonary VEGF, VEGF receptors (VEGFR-1/VEGFR-2), and endothelial NO synthase (eNOS). Neonate Sprague-Dawley rats (CEUA #043/2011) were divided into four groups (n=8 each): control (C), control submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal hypoxia; Mechanical ventilation; VEGF; VEGF receptors; ENOS enzyme.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100608

Investigating ego modules and pathways in osteosarcoma by integrating the EgoNet algorithm and pathway analysis
Provedor de dados: 56
Autores: Chen,X.Y.; Chen,Y.H.; Zhang,L.J.; Wang,Y.; Tong,Z.C.
Osteosarcoma (OS) is the most common primary bone malignancy, but current therapies are far from effective for all patients. A better understanding of the pathological mechanism of OS may help to achieve new treatments for this tumor. Hence, the objective of this study was to investigate ego modules and pathways in OS utilizing EgoNet algorithm and pathway-related analysis, and reveal pathological mechanisms underlying OS. The EgoNet algorithm comprises four steps: constructing background protein-protein interaction (PPI) network (PPIN) based on gene expression data and PPI data; extracting differential expression network (DEN) from the background PPIN; identifying ego genes according to topological features of genes in reweighted DEN; and collecting ego...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteosarcoma; Ego; Genes; Modules; Pathways.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200607

Episodes of water deprivation enhance daily hypertonic NaCl intake in rats
Provedor de dados: 56
Autores: Pereira,D.T.B.; David,R.B.; Menani,J.V.; De Luca Jr.,L.A.
Water and 1.8% NaCl intake was recorded daily in adult male rats (N = 6) submitted to four water deprivations plus four sodium appetite tests, each at the end of each 7-day interval, or in controls (non-deprived, N = 6). Water deprivation was achieved by removing water and 1.8% NaCl for 24 h. Water was then offered for 2 h. At the end of this period, 1.8% NaCl was also offered in addition to water (sodium appetite test). Average daily 1.8% NaCl intake was enhanced from 5.2 ± 1.0 to 15.7 ± 2.5 ml from the first to the fifth week in the experimental group and was unchanged in the control group. Daily water intake was not altered in either group. Thus, repeated episodes of water deprivation enhance daily NaCl intake.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thirst; Salt appetite; Dehydration; Natriuresis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400009

Reinforcement omission effects in rats with bilateral lesions in the substantia nigra pars compacta and ventral tegmental area
Provedor de dados: 56
Autores: Tavares,T.F.; Judice-Daher,D.M.; Bueno,J.L.O.
Reinforcement omission effects (ROEs) are characterized by higher response rates after reinforcement omission than after reinforcement delivery. This pattern of behavior is interpreted in terms of motivational and attentional processes. Recent studies from our laboratory have shown that the amygdala, nucleus accumbens, and medial prefrontal cortex are involved in ROE modulation. Also, the literature has demonstrated a role of other areas such as substantia nigra pars compacta (SNc) and the ventral tegmental area (VTA) in processes related to surprising events, such as prediction error and presentation or omission of an event (exteroceptive stimulus and reinforcement). Since these structures send projections to areas related to ROE modulation such as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reinforcement omission effects; Substantia nigra pars compacta; Ventral tegmental area; Fixed interval with a limited hold; Operant behavior.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700606

Changes in epidemiology of rotavirus in the Triângulo Mineiro region of Brazil: lack of two consecutive rotavirus seasons
Provedor de dados: 56
Autores: Dulgheroff,A.C.B.; Figueiredo,E.F.; Gouvêa,V.S.; Domingues,A.L.S.
Rotaviruses are the main cause of infantile acute diarrhea, and a monovalent (G1P[8]) vaccine against the virus was introduced into the Brazilian National Immunization Program for all infants in March 2006. The objectives of this study were to determine the rate and genotype distribution of rotavirus causing infantile diarrhea in the Triângulo Mineiro region of Brazil during 2011-2012 and to assess the impact of local vaccination. Fecal specimens were analyzed for detection and characterization of rotavirus using polyacrylamide gel electrophoresis, reverse transcription followed by polymerase chain reaction (PCR), and PCR-genotyping assays. Overall, rotavirus was diagnosed in 1.7% (6/348) of cases. Rotavirus positivity rates decreased 88% [95% confidence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Infantile diarrhea; Surveillance; Genotyping.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201091

Side effects of oxysterols: cytotoxicity, oxidation, inflammation, and phospholipidosis
Provedor de dados: 56
Autores: Vejux,A.; Malvitte,L.; Lizard,G.
Oxysterols are 27-carbon atom molecules resulting from autoxidation or enzymatic oxidation of cholesterol. They are present in numerous foodstuffs and have been demonstrated to be present at increased levels in the plasma of patients with cardiovascular diseases and in atherosclerotic lesions. Thus, their role in lipid disorders is widely suspected, and they might also be involved in important degenerative diseases such as Alzheimer's disease, osteoporosis, and age-related macular degeneration. Since atherosclerosis is associated with the presence of apoptotic cells and with oxidative and inflammatory processes, the ability of some oxysterols, especially 7-ketocholesterol and 7β-hydroxycholesterol, to trigger cell death, activate inflammation, and modulate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Atherosclerosis; Inflammation; Oxysterols; Phospholipidosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700001

Distribution of biglycan and decorin in rat dental tissue
Provedor de dados: 56
Autores: Tenório,D.M.H.; Santos,M.F.; Zorn,T.M.T.
Biglycan and decorin are small leucine-rich proteoglycans that play several biological and structural roles in different tissues and organs. Several reports have indicated that biglycan participates in odontoblast and ameloblast differentiation and in the calcification process. In the present study we show that the expression of biglycan changes from within the ameloblasts and odontoblasts to the extracellular space according to the stage of animal development. In predentin and in the pulp space, however, biglycan was continually expressed throughout the period of investigation. In contrast, decorin was absent in odontoblasts and in ameloblasts and was exclusively expressed in predentin throughout the period of observation. In young rats, however, decorin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decorin; Biglycan; Odontoblasts; Predentin; Immunohistochemistry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800012

Comparison of the Polar S810i monitor and the ECG for the analysis of heart rate variability in the time and frequency domains
Provedor de dados: 56
Autores: Vanderlei,L.C.M.; Silva,R.A.; Pastre,C.M.; Azevedo,F.M.; Godoy,M.F.
The aim of the present study was to compare heart rate variability (HRV) at rest and during exercise using a temporal series obtained with the Polar S810i monitor and a signal from a LYNX® signal conditioner (BIO EMG 1000 model) with a channel configured for the acquisition of ECG signals. Fifteen healthy subjects aged 20.9 ± 1.4 years were analyzed. The subjects remained at rest for 20 min and performed exercise for another 20 min with the workload selected to achieve 60% of submaximal heart rate. RR series were obtained for each individual with a Polar S810i instrument and with an ECG analyzed with a biological signal conditioner. The HRV indices (rMSSD, pNN50, LFnu, HFnu, and LF/HF) were calculated after signal processing and analysis. The unpaired...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart rate variability; Autonomic nervous system; Exercise; Spectral analysis; ECG; Polar S810i monitor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000004

Psychological stress alters the ultrastructure and increases IL-1β and TNF-α in mandibular condylar cartilage
Provedor de dados: 56
Autores: Lv,Xin; Li,Qiang; Wu,Shun; Sun,Jing; Zhang,Min; Chen,Yong-Jin.
Psychological factors can be correlated with temporomandibular disorders (TMDs), but the mechanisms are unknown. In the present study, we examined the microstructural changes and expression of proinflammatory cytokines in mandibular condylar cartilage of the temporomandibular joint (TMJ) in a psychological stress animal model. Male Sprague-Dawley rats (8 weeks old, 210 ± 10 g) were randomly divided into 3 groups: psychological stress (PS, N = 48), foot shock (FS, N = 24), and control (N = 48). After inducing psychological stress using a communication box with the FS rats for 1, 3, or 5 weeks, PS rats were sacrificed and compared to their matched control littermates, which received no stress and were killed at the same times as the PS rats. Body and adrenal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychological stress; Temporomandibular joint; IL-1β; TNF-α.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000013

Overexpression of ezrin and galectin-3 as predictors of poor prognosis of cervical cancer
Provedor de dados: 56
Autores: Li,M.; Feng,Y.M.; Fang,S.Q.
The aim of this study was to explore the correlation of ezrin and galectin-3 expressions with prognosis in cervical cancer. The immunohistochemical method was applied to detect ezrin and galectin-3 expressions in normal cervix tissues (n=30), cervicitis tissues (n=28), cervical intraepithelial neoplasia (CIN) tissues (classified as I-III, n=89), and cervical carcinoma tissues (n=84). Follow-up was conducted for 5 to 78 months to analyze the correlation of protein expressions with prognosis. Ezrin and galectin-3 expressions in cervical cancer were significantly higher than in normal cervix, cervicitis and CIN (all P&lt;0.05), and expressions in CIN were significantly higher than in normal cervix and cervicitis (both P&lt;0.05). The expressions of ezrin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ezrin protein; Galectin-3 protein; Cervical cancer; Deep myometrial invasion; Lymph node metastasis; Prognosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400601

Circulating levels of inflammation-associated miR-155 and endothelial-enriched miR-126 in patients with end-stage renal disease
Provedor de dados: 56
Autores: Wang,Honglei; Peng,Wujian; Shen,Xuemei; Huang,Yunhui; Ouyang,Xin; Dai,Yong.
Circulating microRNAs (miRNAs) may represent a potential noninvasive molecular biomarker for various pathological conditions. Moreover, the detection of circulating miRNAs can provide important novel disease-related information. In particular, inflammation-associated miR-155 and endothelial-enriched miR-126 are reported to be associated with vascular homeostasis. Vascular damage is a common event described in end-stage renal disease (ESRD). We hypothesized that miR-155 and miR-126 may be detectable in the circulation and serve as potential biomarkers for risk stratification. In this study, we assessed miR-155 and miR-126 in the plasma of 30 ESRD patients and 20 healthy controls using real-time quantification RT-PCR. The circulating levels of miR-155 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNAs; End-stage renal disease; Hemodialysis; MiR-126; MiR-155.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200029

Effects of a neuronal nitric oxide synthase inhibitor on lipopolysaccharide-induced fever
Provedor de dados: 56
Autores: Perotti,C.A.A.; Nogueira,M.S.; Antunes-Rodrigues,J.; Cárnio,E.C.
It has been demonstrated that nitric oxide (NO) has a thermoregulatory action, but very little is known about the mechanisms involved. In the present study we determined the effect of neuronal nitric oxide synthase (nNOS) inhibition on thermoregulation. We used 7-nitroindazole (7-NI, 1, 10 and 30 mg/kg body weight), a selective nNOS inhibitor, injected intraperitoneally into normothermic Wistar rats (200-250 g) and rats with fever induced by lipopolysaccharide (LPS) (100 µg/kg body weight) administration. It has been demonstrated that the effects of 30 mg/kg of 7-NI given intraperitoneally may inhibit 60% of nNOS activity in rats. In all experiments the colonic temperature of awake unrestrained rats was measured over a period of 5 h at 15-min intervals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; 7-nitroindazole; Body temperature; LPS; L-NAME.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100008

Prevalence of genital human papillomavirus among rural and urban populations in southern Yunnan province, China
Provedor de dados: 56
Autores: Baloch,Z.; Yuan,T.; Yindi,S.; Feng,Y.; Tai,W.; Liu,Y.; Liu,L.; Zhang,A.; Wang,B.; Wu,X.; Xia,X.
This study was designed to investigate and compare the HPV prevalence, genotypes distribution and associated risk factors in rural and urban women living in Xishuang Banna district, in the province of Yunnan. A total of 177 and 190 women from rural and urban areas were engaged, respectively. HPV DNA was amplified using the L1 consensus primers system (MY09/11 and GP5/6) and HPV GenoArray test was conducted for genotyping. Proportions were compared by chi-square test, and logistic regression was used to evaluate risk factors. A total of 54 women were positive for HPV DNA. Among rural women, 23 women were positive for HPV infection, of which 21 showed a single infection and 2 had a multiple infection. HPV-16 (10/23) was the most prevalent genotype followed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xishuang Banna; Prevalence; Genotype; Oncogenic; Rural; Urban.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600708

Propofol inhibits invasion and growth of ovarian cancer cells via regulating miR-9/NF-κB signal
Provedor de dados: 56
Autores: Huang,X.; Teng,Y.; Yang,H.; Ma,J.
Propofol is one of the most commonly used intravenous anesthetic agents during cancer resection surgery. A previous study has found that propofol can inhibit invasion and induce apoptosis of ovarian cancer cells. However, the underlying mechanisms are not known. miR-9 has been reported to be little expressed in ovarian cancer cells, which has been related to a poor prognosis in patients with ovarian cancer. Studies have also demonstrated that propofol could induce microRNAs expression and suppress NF-κB activation in some situations. In the present study, we assessed whether propofol inhibits invasion and induces apoptosis of ovarian cancer cells by miR-9/NF-κB signaling. Ovarian cancer ES-2 cells were transfected with anti-miR-9 or p65 cDNA or p65 siRNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; Ovarian cancer; MiR-9; NF-κB; Apoptosis; Invasion.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200605

Cyclosporin inhibits hyperalgesia and edema in arthritic rats: role of the central nervous system
Provedor de dados: 56
Since arthritis induced by Mycobacterium products (adjuvant) in rats is considered to be immunologically driven, the objective of the present study was to determine if the immunosuppressor drug cyclosporin could affect hindpaw edema and joint hyperalgesia simultaneously. Female Holtzman rats (140-170 g) presented hyperalgesia and edema on the 8th and 12th day following adjuvant injection. Daily systemic (oral or intramuscular) administration of cyclosporin (0.5-5.0 mg kg-1 day-1) or dexamethasone (0.01-0.1 mg kg-1 day-1) for 15 days starting on day zero dose-dependently inhibited the hindpaw edema and hyperalgesia in arthritic rats. However, hyperalgesia but not edema could be detected two days after cyclosporin withdrawal. We concluded that a) the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cyclosporin; Hyperalgesia; Edema; Adjuvant arthritis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100016

Triorganotin as a compound with potential reproductive toxicity in mammals
Provedor de dados: 56
Autores: Delgado Filho,V.S.; Lopes,P.F.I.; Podratz,P.L.; Graceli,J.B.
Organotin compounds are typical environmental contaminants and suspected endocrine-disrupting substances, which cause irreversible sexual abnormality in female mollusks, called "imposex". However, little is known about the capability of triorganotin compounds, such as tributyltin and triphenyltin, to cause disorders in the sexual development and reproductive functions of mammals, including humans and rodents. Moreover, these compounds can act as potential competitive inhibitors of aromatase enzyme and other steroidogenic enzymes, affecting the reproductive capacity of male and female mammals. In this review, we discuss the cellular, biochemical, and molecular mechanisms by which triorganotin compounds induce adverse effects in the mammalian reproductive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tributyltin; Triphenyltin; Endocrine disruptor; Aromatase; Imposex; Mammalian reproductive organs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900018

Heterotopic transplantation of glycerin-preserved trachea: effect of respiratory epithelium desquamation on acute rejection
Provedor de dados: 56
Autores: Saueressig,M.G.; Edelweiss,M.I.A.; Souza,F.H.; Moreschi,A.H.; Savegnago,F.L.; Macedo Neto,A.V.
An effective preservation method and decreased rejection are essential for tracheal transplantation in the reconstruction of large airway defects. Our objective in the present study was to evaluate the antigenic properties of glycerin-preserved tracheal segments. Sixty-one tracheal segments (2.4 to 3.1 cm) were divided into three groups: autograft (N = 21), fresh allograft (N = 18) and glycerin-preserved allograft (N = 22). Two segments from different groups were implanted into the greater omentum of dogs (N = 31). After 28 days, the segments were harvested and analyzed for mononuclear infiltration score and for the presence of respiratory epithelium. The fresh allograft group presented the highest score for mononuclear infiltration (1.78 ± 0.43, P &lt;=...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Graft rejection; Trachea; Transplantation; Glycerol; Respiratory mucosa.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700010

Retraction notice for: Characteristics of liver fibrosis with different etiologies using a fully quantitative fibrosis assessment tool [Braz J Med Biol Res (2017) 50(6): e5234]
Provedor de dados: 56
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700951

Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture
Provedor de dados: 56
Autores: Conceição,R.A.; Ludovico,M.S.; Andrade,C.G.T.J.; Yano,T.
The adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%). In addition, bacteria concentrations of approximately 2 x 10(7) CFU/mL were recovered from Vero cells following an invasion assay. Furthermore, PCR analysis of adhesin genes showed that 98.0% of these SEPEC strains tested positive for fimH, 69.4% for flu, 53.1% for csgA, 38.8% for mat, and 32.7% for iha. Analysis of the invasin genes showed that 16.3% of the SEPEC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Invasiveness; Escherichia coli; Sepsis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500007

The effect of feeding on the respiratory activity of the sloth
Provedor de dados: 56
Autores: Pedrosa,M.A.C.; Lima,A.M.J.; Bezerra,A.P.; Duarte,D.P.F.; Da-Costa,C.P.
The aim of the present study was to confirm whether feeding influences the resting breathing rate and to observe possible alterations in blood gas and pH levels produced by feeding in unanesthetized sloths (Bradypus variegatus). Five adult male sloths (4.1 ± 0.6 kg) were placed daily in an experimental chair for a period of at least 4 h for sitting adaptation. Five measurements were made for each sloth. However, the sloths one, two and five were studied once and the sloths three and four were studied twice. Breathing rate was determined with an impedance meter and the output signal was digitized. Arterial blood samples were collected for blood gas analysis with a BGE electrolytes analyzer and adjusted for the animal's body temperature and hemoglobin...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sloth; Bradypus variegatus; Feeding; Respiratory activity; Breathing rate; Arterial blood pH; PCO2 and PO2 levels.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700014

Amniotic fluid amino acid levels in non-immune hydrops fetalis: a case-control study
Provedor de dados: 56
Autores: Erdemoğlu,M.; Kuyumcuoglu,U.; Guzel,A.I.; Celik,Y.; Kale,E.
In a prospective case-control study, we compared the amniotic fluid amino acid levels in non-immune hydrops fetalis (NIHF) and normal fetuses. Eighty fetuses underwent amniocentesis for different reasons at the prenatal diagnosis unit of the Department of Obstetrics and Gynecology, Faculty of Medicine, Dicle University. Forty of these fetuses were diagnosed with NIHF. The study included 40 women each in the NIHF (mean age: 27.69 ± 4.56 years) and control (27.52 ± 5.49 years) groups, who had abnormal double- or triple-screening test values with normal fetuses with gestational ages of 23.26 ± 1.98 and 23.68 ± 1.49 weeks at the time of sample collection, respectively. Amniotic fluid amino acid concentrations (intra-assay variation: 2.26-7.85%; interassay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-immune hydrops fetalis; Amniotic fluid; Amino acid levels.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700018

Microsatellite instability and cytogenetic survey in myeloid leukemias
Provedor de dados: 56
Autores: Ribeiro,E.M.S.F.; Rodriguez,J.M.; Cóser,V.M.; Sotero,M.G.; Fonseca Neto,J.M.; Pasquini,R.; Cavalli,I.J.
Microsatellites are short tandem repeat sequences dispersed throughout the genome. Their instability at multiple genetic loci may result from mismatch repair errors and it occurs in hereditary nonpolyposis colorectal cancer. This instability is also found in many sporadic cancers. In order to evaluate the importance of this process in myeloid leukemias, we studied five loci in different chromosomes of 43 patients, 22 with chronic myelocytic leukemia (CML) in the chronic phase, 7 with CML in blast crisis, and 14 with acute myeloid leukemia (AML), by comparing leukemic DNA extracted from bone marrow and constitutional DNA obtained from buccal epithelial cells. Only one of the 43 patients (2.1%), with relapsed AML, showed an alteration in the allele length at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomic instability; Mismatch repair errors; Cytogenetics; Leukemogenesis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200003

Hyperthermia induced after recirculation triggers chronic neurodegeneration in the penumbra zone of focal ischemia in the rat brain
Provedor de dados: 56
Autores: Favero-Filho,L.A.; Borges,A.A.; Grassl,C.; Lopes,A.C.; Sinigaglia-Coimbra,R.; Coimbra,C.G.
Chronic neurodegenerative processes have been identified in the rat forebrain after prolonged survival following hyperthermia (HT) initiated a few hours after transient global ischemia. Since transient global ischemia and ischemic penumbra share pathophysiological similarities, this study addressed the effects of HT induced after recirculation of focal brain ischemia on infarct size during long survival times. Adult male Wistar rats underwent intra-luminal occlusion of the left middle cerebral artery for 60 min followed by HT (39.0-39.5°C) or normothermia. Control procedures included none and sham surgery with and without HT, and middle cerebral artery occlusion alone. Part I: 6-h HT induced at recirculation. Part II: 2-h HT induced at 2-, 6-, or 24-h...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperthermia; Ischemic penumbra; Neurodegeneration; Rat focal ischemia.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100014

Effect of skilled and unskilled training on nerve regeneration and functional recovery
Provedor de dados: 56
Autores: Pagnussat,A.S.; Michaelsen,S.M.; Achaval,M.; Ilha,J.; Hermel,E.E.S.; Back,F.P.; Netto,C.A.
The most disabling aspect of human peripheral nerve injuries, the majority of which affect the upper limbs, is the loss of skilled hand movements. Activity-induced morphological and electrophysiological remodeling of the neuromuscular junction has been shown to influence nerve repair and functional recovery. In the current study, we determined the effects of two different treatments on the functional and morphological recovery after median and ulnar nerve injury. Adult Wistar male rats weighing 280 to 330 g at the time of surgery (N = 8-10 animals/group) were submitted to nerve crush and 1 week later began a 3-week course of motor rehabilitation involving either "skilled" (reaching for small food pellets) or "unskilled" (walking on a motorized treadmill)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peripheral nerve injury; Functional recovery; Median nerve; Ulnar nerve; Nerve morphometry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800010

Decreased left temporal lobe volume of panic patients measured by magnetic resonance imaging
Provedor de dados: 56
Autores: Uchida,R.R.; Del-Ben,C.M.; Santos,A.C.; Araújo,D.; Crippa,J.A.; Guimarães,F.S.; Graeff,F.G.
Reported neuroimaging studies have shown functional and morphological changes of temporal lobe structures in panic patients, but only one used a volumetric method. The aim of the present study was to determine the volume of temporal lobe structures in patients with panic disorder, measured by magnetic resonance imaging. Eleven panic patients and eleven controls matched for age, sex, handedness, socioeconomic status and years of education participated in the study. The mean volume of the left temporal lobe of panic patients was 9% smaller than that of controls (t21 = 2.37, P = 0.028). In addition, there was a trend (P values between 0.05 and 0.10) to smaller volumes of the right temporal lobe (7%, t21 = 1.99, P = 0.06), right amygdala (8%, t21 = 1.83, P =...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Panic disorder; Temporal lobe; Magnetic resonance imaging; Volumetric analysis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700014

Long-term efficacy of a rural community-based integrated intervention for prevention and management of chronic obstructive pulmonary disease: a cluster randomized controlled trial in China's rural areas
Provedor de dados: 56
Autores: Yuan,X.; Tao,Y.; Zhao,J.P.; Liu,X.S.; Xiong,W.N.; Xie,J.G.; Ni,W.; Xu,Y.J.; Liu,H.G.
This study aimed to assess the efficacy of a rural community-based integrated intervention for early prevention and management of chronic obstructive pulmonary disease (COPD) in China. This 18-year cluster-randomized controlled trial encompassing 15 villages included 1008 patients (454 men and 40 women in the intervention group [mean age, 54 ± 10 years]; 482 men and 32 women in the control group [mean age, 53 ± 10 years]) with confirmed COPD or at risk for COPD. Villages were randomly assigned to the intervention or the control group, and study participants residing within the villages received treatment accordingly. Intervention group patients took part in a program that included systematic health education, smoking cessation counseling, and education on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Randomized controlled trial; Integrated intervention; Rural area; Lung function; Smoking cessation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101023

Use of the Wasserman equation in optimization of the duration of the power ramp in a cardiopulmonary exercise test: a study of Brazilian men
Provedor de dados: 56
Autores: Costa,D. C.; Santi,G. L. de; Crescêncio,J. C.; Seabra,L. P.; Carvalho,E. E. V.; Papa,V.; Marques,F.; Gallo Junior,L.; Schmidt,A.
This study aimed to analyze the agreement between measurements of unloaded oxygen uptake and peak oxygen uptake based on equations proposed by Wasserman and on real measurements directly obtained with the ergospirometry system. We performed an incremental cardiopulmonary exercise test (CPET), which was applied to two groups of sedentary male subjects: one apparently healthy group (HG, n=12) and the other had stable coronary artery disease (n=16). The mean age in the HG was 47±4 years and that in the coronary artery disease group (CG) was 57±8 years. Both groups performed CPET on a cycle ergometer with a ramp-type protocol at an intensity that was calculated according to the Wasserman equation. In the HG, there was no significant difference between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wasserman equation; Cardiopulmonary exercise test; Coronary artery disease; Cardiovascular diagnostic techniques.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201136

Arterial blood pressure of term newborns during the first week of life
Provedor de dados: 56
Autores: Nascimento,M.C.V.A.; Xavier,C.C.; Goulart,E.M.A.
The progressive behavior of the blood pressure of term newborns during the first week of life was assessed by the simultaneous use of oscillometric and Doppler methods. A total of 174 term neonates born at the Municipal Hospital Odilon Behrens in Belo Horizonte, from March 1996 to February 1997, were prospectively assessed. The oscillometric and Doppler ultrasonic methods were simultaneously used for four consecutive recordings obtained at 12 ± 6, 24 ± 6 and 72 ± 24 h and on the 7th ± 1 day of life. The combined use of the two methods simplified the procedure, with automatic cuff inflation and deflation, and speed was properly controlled with an automatic pressure monitor. The procedure was performed using a Y-connection to the mercury sphygmomanometer,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood pressure; Doppler method; Oscillometric method; Term newborn.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800007

Regulation of the kinin receptors after induction of myocardial infarction: a mini-review
Provedor de dados: 56
Autores: Tschöpe,C.; Heringer-Walther,S.; Walther,T.
It is well known that the responses to vasoactive kinin peptides are mediated through the activation of two receptors termed bradykinin receptor B1 (B1R) and B2 (B2R). The physiologically prominent B2R subtype has certainly been the subject of more intensive efforts in structure-function studies and physiological investigations. However, the B1R activated by a class of kinin metabolites has emerged as an important subject of investigation within the study of the kallikrein-kinin system (KKS). Its inducible character under stress and tissue injury is therefore a field of major interest. Although the KKS has been associated with cardiovascular regulation since its discovery at the beginning of the last century, less is known about the B1R and B2R regulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B1 receptor; B2 receptor; Bradykinin; Heart infarction; Kallikrein-kinin system.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600011

Negative correlation between phospholipase and esterase activity produced by Fusarium isolates
Provedor de dados: 56
Autores: Ishida,K.; Alviano,D.S.; Silva,B.G.; Guerra,C.R.; Costa,A.S.; Nucci,M.; Alviano,C.S.; Rozental,S.
Fusarium species have emerged as one of the more outstanding groups of clinically important filamentous fungi, causing localized and life-threatening invasive infections with high morbidity and mortality. The ability to produce different types of hydrolytic enzymes is thought to be an important virulence mechanism of fungal pathogens and could be associated with the environment of the microorganism. Here, we have measured the production of two distinct lipolytic enzymes, phospholipase and esterase, by sixteen Fusarium isolates recovered from the hospital environment, immunocompromised patients’ blood cultures, foot interdigital space scrapings from immunocompromised patients, and foot interdigital space scrapings from immunocompetent patients (4 isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium; Hospital isolates; Phospholipase; Esterase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500006

Rosuvastatin prevents myocardial necrosis in an experimental model of acute myocardial infarction
Provedor de dados: 56
Autores: Dourado,P.M.M.; Tsutsui,J.M.; Landim,M.B.P.; Casella Filho,A.; Galvao,T.F.G.; Aiello,V.D.; Mathias Jr.,W.; da Luz,P.L.; Chagas,A.C.P.
Dyslipidemia is related to the progression of atherosclerosis and is an important risk factor for acute coronary syndromes. Our objective was to determine the effect of rosuvastatin on myocardial necrosis in an experimental model of acute myocardial infarction (AMI). Male Wistar rats (8-10 weeks old, 250-350 g) were subjected to definitive occlusion of the left anterior descending coronary artery to cause AMI. Animals were divided into 6 groups of 8 to 11 rats per group: G1, normocholesterolemic diet; G2, normocholesterolemic diet and rosuvastatin (1 mg·kg-1·day-1) 30 days after AMI; G3, normocholesterolemic diet and rosuvastatin (1 mg·kg-1·day-1) 30 days before and after AMI; G4, hypercholesterolemic diet; G5, hypercholesterolemic diet and rosuvastatin (1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myocardial infarction; Rosuvastatin; Hypercholesterolemia; Myocardial necrosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500013

Inhibition of return, gap effect and saccadic reaction time to a visual target
Provedor de dados: 56
Autores: Guimarães-Silva,S.; Gawryszewski,L.G.; Portugal,T.S.; Klausner-de-Oliveira,L.
Simple manual reaction time (MRT) to a visual target (S2) is shortened when a non-informative cue (S1) is flashed at the S2 location shortly before the onset of S2 (early facilitation). Afterwards, MRT to S2 appearing at the S1 location is lengthened (inhibition of return - IOR). Similar results have been obtained for saccadic reaction time (SRT). Moreover, when there is a temporal gap between offset of the fixation point (FP) and onset of a target (gap paradigm), SRT is shorter than SRT in an overlap paradigm (FP remains on). In the present study, we determined SRT to S2 (10º) after presenting S1 at the same eccentricity (10º) or at a parafoveal position (2º) in the same or in the opposite hemifield. In addition, we employed both gap and overlap...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Visual attention; Saccadic reaction time; Inhibition of return; Covert orienting; Gap effect.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400010

Influence of exercise on the activity and the distribution between free and bound forms of glycolytic and associated enzymes in tissues of horse mackerel
Provedor de dados: 56
Autores: Lushchak,V.I.; Bagnyukova,T.V.; Storey,J.M.; Storey,K.B.
The effects of short-term burst (5 min at 1.8 m/s) swimming and long-term cruiser (60 min at 1.2 m/s) swimming on maximal enzyme activities and enzyme distribution between free and bound states were assessed for nine glycolytic and associated enzymes in tissues of horse mackerel, Trachurus mediterraneus ponticus. The effects of exercise were greatest in white muscle. The activities of phosphofructokinase (PFK), pyruvate kinase (PK), fructose-1,6-bisphosphatase (FBPase), and phosphoglucomutase (PGM) all decreased to 47, 37, 37 and 67%, respectively, during 60-min exercise and all enzymes except phosphoglucoisomerase (PGI) and PGM showed a change in the extent of binding to subcellular particulate fractions during exercise. In red muscle, exercise affected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trachurus mediterraneus; Swimming exercise; Glycolysis; Subcellular enzyme binding.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800013

Subacute effects of a maximal exercise bout on endothelium-mediated vasodilation in healthy subjects
Provedor de dados: 56
Autores: Bousquet-Santos,K.; Soares,P.P.S.; Nóbrega,A.C.L.
We evaluated vascular reactivity after a maximal exercise test in order to determine whether the effect of exercise on the circulation persists even after interruption of the exercise. Eleven healthy sedentary volunteers (six women, age 28 ± 5 years) were evaluated before and after (10, 60, and 120 min) a maximal exercise test on a treadmill. Forearm blood flow (FBF) was measured by venous occlusion plethysmography before and during reactive hyperemia (RH). Baseline FBF, analyzed by the area under the curve, increased only at 10 min after exercise (P = 0.01). FBF in response to RH increased both at 10 and 60 min vs baseline (P = 0.004). Total excess flow for RH above baseline showed that vascular reactivity was increased up to 60 min after exercise (mean ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vascular reactivity; Exercise test; Blood flow; Plethysmography.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400017

Familial history of hypertension-induced impairment on heart rate variability was not observed in strength-trained subjects
Provedor de dados: 56
Autores: Santa-Rosa,F.A.; Shimojo,G.L.; Sartori,M.; Rocha,A.C.; Francica,J.V.; Paiva,J.; Irigoyen,M.C.; De Angelis,K.
Family history of hypertension is an important predictive factor for hypertension and is associated with hemodynamic and autonomic abnormalities. Previous studies reported that strength training might reduce arterial blood pressure (AP), as well as improve heart rate variability (HRV). However, the benefits of strength training in the offspring of hypertensive parents have not been fully evaluated. Here, we analyzed the impact of strength training on hemodynamics and autonomic parameters in offspring of hypertensive subjects. We performed a cross-sectional study with sedentary or physically active offspring of normotensives (S-ON and A-ON) or hypertensives (S-OH and A-OH). We recorded RR interval for analysis of HRV. AP was similar between groups....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Family history; Arterial blood pressure; Cardiac autonomic modulation; Sympathetic modulation; Strength training.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200605

Modulation of rhodopsin gene expression and signaling mechanisms evoked by endothelins in goldfish and murine pigment cell lines
Provedor de dados: 56
Autores: Lopes,G.J.D.; Góis,C.C.; Lima,L.H.R.G.; Castrucci,A.M.L.
Endothelins (ETs) and sarafotoxins (SRTXs) belong to a family of vasoconstrictor peptides, which regulate pigment migration and/or production in vertebrate pigment cells. The teleost Carassius auratus erythrophoroma cell line, GEM-81, and Mus musculus B16 melanocytes express rhodopsin, as well as the ET receptors, ETB and ETA, respectively. Both cell lines are photoresponsive, and respond to light with a decreased proliferation rate. For B16, the doubling time of cells kept in 14-h light (14L):10-h darkness (10D) was higher compared to 10L:14D, or to DD. The doubling time of cells kept in 10L:14D was also higher compared to DD. Using real-time PCR, we demonstrated that SRTX S6c (12-h treatment, 100 pM and 1 nM; 24-h treatment, 1 nM) and ET-1 (12-h...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhodopsin; Sarafotoxin; Endothelin; Pigment cells; Gene expression; Signaling pathway.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900004

Mutations in SRY and WT1 genes required for gonadal development are not responsible for XY partial gonadal dysgenesis
Provedor de dados: 56
Autores: Tagliarini,E.B.; Assumpção,J.G.; Scolfaro,M.R.; Mello,M.P. de; Maciel-Guerra,A.T.; Guerra Júnior,G.; Hackel,C.
The WT1 transcription factor regulates SRY expression during the initial steps of the sex determination process in humans, activating a gene cascade leading to testis differentiation. In addition to causing Wilms' tumor, mutations in WT1 are often responsible for urogenital defects in men, while SRY mutations are mainly related to 46,XY pure gonadal dysgenesis. In order to evaluate their role in abnormal testicular organogenesis, we screened for SRY and WT1 gene mutations in 10 children with XY partial gonadal dysgenesis, 2 of whom with a history of Wilms' tumor. The open reading frame and 360 bp of the 5' flanking sequence of the SRY gene, and the ten exons and intron boundaries of the WT1 gene were amplified by PCR of genomic DNA. Single-strand...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: XY partial gonadal dysgenesis; SRY open reading frame; SRY 5' untranslated region; WT1 exons; Denys-Drash syndrome.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100004

Cardiovascular effects of the intracerebroventricular injection of adrenomedullin: roles of the peripheral vasopressin and central cholinergic systems
Provedor de dados: 56
Autores: Cam-Etoz,B.; Isbil-Buyukcoskun,N.; Ozluk,K.
Our objective was to investigate in conscious Sprague-Dawley (6-8 weeks, 250-300 g) female rats (N = 7 in each group) the effects of intracerebroventricularly (icv) injected adrenomedullin (ADM) on blood pressure and heart rate (HR), and to determine if ADM and calcitonin gene-related peptide (CGRP) receptors, peripheral V1 receptors or the central cholinergic system play roles in these cardiovascular effects. Blood pressure and HR were observed before and for 30 min following drug injections. The following results were obtained: 1) icv ADM (750 ng/10 µL) caused an increase in both blood pressure and HR (DMAP = 11.8 ± 2.3 mmHg and ΔHR = 39.7 ± 4.8 bpm). 2) Pretreatment with a CGRP receptor antagonist (CGRP8-37) and ADM receptor antagonist (ADM22-52)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenomedullin; Blood pressure; Vasopressin; Atropine; Mecamylamine.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300011

Anhydrobiosis in yeast: activation effect
Provedor de dados: 56
Intracellular substances released into the medium during rehydration of dry yeast cells can significantly improve the quality of a synthetic medium. Acceleration of yeast growth in this medium and increased yield of biomass are observed simultaneously. The change in the molecular arrangement of intracellular membranes as a result of the strong dehydration of live organisms is a negative phenomenon that reduces the level of cell viability. However, this phenomenon also represents an adaptive mechanism which facilitates the maintenance of population viability as a whole under extreme environmental conditions
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Yeasts; Anhydrobiosis; Dehydration-rehydration; Plasma membrane molecular organization.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100002

Comparison of serum hormone levels of captive and free-living maned wolves Chrysocyon brachyurus
Provedor de dados: 56
Autores: Maia,O.B.; Jácomo,A.T.A.; Bringel,B.A.; Kashivakura,C.K.; Oliveira,C.A.; Teodoro,L.O.F.; Silveira,L.; Teixeira da Costa,M.E.L.; Malta,M.C.C.; Furtado,M.M.; Torres,N.M.; Mattos,P.S.R.; Viau,.P; Lima,T.F.G.; Morato,R.G.
Serum hormone levels were compared between captive and free-living maned wolves and seasonal variations of sex hormones were studied. Blood samples were collected from 16 male and 26 female adult animals from Brazilian zoos, and from 30 male and 24 female free-living adults to determine serum progesterone and testosterone by radioimmunoassay. Serum testosterone concentrations varied (P &lt; 0.05) across seasons for 16 captive males, being higher in autumn (2184.7 ± 355.1 pg/mL) than in summer (1080.7 ± 205.4 pg/mL), winter (1270.1 ± 276.6 pg/mL) and spring (963.9 ± 248.1 pg/mL), although they did not differ between summer, winter and spring. Testosterone concentration of 30 free-living males differed (P &lt; 0.05) between autumn (824.1 ± 512.2 pg/mL),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Maned wolf Chrysocyon brachyurus; Progesterone; Testosterone; Radioimmunoassay; Reproduction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200015

Tyrosine phosphorylation of Kv1.5 is upregulated in intrauterine growth retardation rats with exaggerated pulmonary hypertension
Provedor de dados: 56
Autores: Fu,L.C.; Lv,Y.; Zhong,Y.; He,Q.; Liu,X.; Du,L.Z.
Intrauterine growth retardation (IUGR) is associated with the development of adult-onset diseases, including pulmonary hypertension. However, the underlying mechanism of the early nutritional insult that results in pulmonary vascular dysfunction later in life is not fully understood. Here, we investigated the role of tyrosine phosphorylation of voltage-gated potassium channel 1.5 (Kv1.5) in this prenatal event that results in exaggerated adult vascular dysfunction. A rat model of chronic hypoxia (2 weeks of hypoxia at 12 weeks old) following IUGR was used to investigate the physiological and structural effect of intrauterine malnutrition on the pulmonary artery by evaluating pulmonary artery systolic pressure and vascular diameter in male rats. Kv1.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IUGR; Kv1.5; PASMCs; Pulmonary hypertension; Tyrosine phosphorylation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100604

Residence of liquids in the infra-junctional portion of the proximal stomach in patients with gastroesophageal reflux disease
Provedor de dados: 56
Autores: Barbieri,C.L.A.; Troncon,L.E.A.; Herculano Jr.,J.R.L.; Aprile,L.R.O.; Dantas,R.O.
Patients with gastroesophageal reflux disease may have disturbances of gastric motility, which could play a role in the pathophysiology of the disease. Recent studies have suggested that the gastric region just below the gastroesophageal junction may have a distinct physiological behavior. We determined whether patients with gastroesophageal reflux disease have abnormal residence of food in the infra-junctional portion of the stomach after ingesting a liquid nutrient meal. Fasted adult patients with reflux disease (N = 11) and healthy volunteers (N = 10) ingested a liquid meal (320 ml; 437 kcal) labeled with 99m technetium-phytate and their total gastric emptying half-time and regional emptying from the stomach infra-junctional region were determined. In 8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroesophageal reflux disease; Gastric emptying; Gastric motility; Intragastric distribution; Proximal stomach.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900012

Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
Provedor de dados: 56
Autores: Cónsole,G.M.; Jurado,S.B.; Oyhenart,E.; Ferese,C.; Pucciarelli,H.; Gómez Dumm,C.L.A.
Undernutrition elicited by a low-protein diet determines a marked reduction of hypophyseal activity and affects the function of the respective target organs. The objective of the present investigation was to study the ultrastructural and quantitative immunohistochemical changes of the different pituitary cell populations in undernourished monkeys that had been previously shown to have significant changes in craniofacial growth. Twenty Saimiri sciureus boliviensis monkeys of both sexes were used. The animals were born in captivity and were separated into two groups at one year of age, i.e., control and undernourished animals. The monkeys were fed ad libitum a 20% (control group) and a 10% (experimental group) protein diet for two years. Pituitaries were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary gland; Quantitative immunohistochemistry; Ultrastructure; Undernutrition; Squirrel monkeys.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100008

Steps in the formation of neurites and synapses studied in cultured leech neurons
Provedor de dados: 56
Autores: De-Miguel,F.F.
Leech neurons in culture have provided novel insights into the steps in the formation of neurite outgrowth patterns, target recognition and synapse formation. Identified adult neurons from the central nervous system of the leech can be removed individually and plated in culture under well-controlled conditions, where they retain their characteristic physiological properties, grow neurites and form specific chemical or electrical synapses. Different identified neurons develop distinctive outgrowth patterns that depend on their identities and on the molecular composition of the substrate. On native substrates, the patterns displayed by these neurons reproduce characteristics from the adult or the developing neurons. In addition, the substrate may induce...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Regeneration; Neurite outgrowth; Synapse formation; Cultured neurons; Leech.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500002

Bevacizumab and gefitinib enhanced whole-brain radiation therapy for brain metastases due to non-small-cell lung cancer
Provedor de dados: 56
Autores: Yang,R.F.; Yu,B.; Zhang,R.Q.; Wang,X.H.; Li,C.; Wang,P.; Zhang,Y.; Han,B.; Gao,X.X.; Zhang,L.; Jiang,Z.M.
Non-small-cell lung cancer (NSCLC) patients who experience brain metastases are usually associated with poor prognostic outcomes. This retrospective study proposed to assess whether bevacizumab or gefitinib can be used to improve the effectiveness of whole brain radiotherapy (WBRT) in managing patients with brain metastases. A total of 218 NSCLC patients with multiple brain metastases were retrospectively included in this study and were randomly allocated to bevacizumab-gefitinib-WBRT group (n=76), gefitinib-WBRT group (n=77) and WBRT group (n=75). Then, tumor responses were evaluated every 2 months based on Response Evaluation Criteria in Solid Tumors version 1.0. Karnofsky performance status and neurologic examination were documented every 6 months after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-small-cell lung cancer; Brain metastasis; Bevacizumab; Gefitinib; Whole brain radiotherapy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100606

Raising awareness of chronic kidney disease in a Brazilian urban population
Provedor de dados: 56
Autores: Mazza Nascimento,M.; Riella,M.C.
The incidence and prevalence of chronic kidney disease have been increasing in recent years in developing countries. The aim of this study was to report the results of a general chronic kidney disease awareness program applied to an urban population in a large Brazilian city. From January 2002 to January 2005 a total of 8883 individuals in the city of Curitiba (PR, Brazil) were screened for hypertension, body mass index, hematuria, and proteinuria. A family history and previous medical diagnosis of hypertension and diabetes mellitus (DM) were also recorded. Of the 8883 individuals assessed, 56% were women, subject median age was 47 years (range: 17-93 years) and more than 90% were Caucasian. Thirty percent had signs of hematuria, 6% had proteinuria, and 3%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematuria; Proteinuria; Prevention; Chronic kidney disease; Screening.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800010

Plasma hydroxy-metronidazole/ metronidazole ratio in hepatitis C virus-induced liver disease
Provedor de dados: 56
Autores: Marchioretto,M.A.M.; Ecclissato,C.; Silva,C.M.F. da; Cassiano,N.M.; Calafatti,S.A.; Mendonça,S.; Ribeiro,M.L.; Bernasconi,G.C.R.; Degger,M.F.; Piovesan,H.; Pedrazzoli Jr.,J.
It has been suggested that the measurement of metronidazole clearance is a sensitive method for evaluating liver function. The aim of this study was to evaluate the usefulness of plasma hydroxy-metronidazole/metronidazole ratios as indicators of dynamic liver function to detect changes resulting from the various forms of chronic hepatitis C virus (HCV) infection. A total of 139 individuals were studied: 14 healthy volunteers, 22 healthy, asymptomatic, consecutive anti-HCV-positive HCV-RNA negative subjects, 81 patients with chronic hepatitis C (49 with moderate/severe chronic hepatitis and 34 with mild hepatitis), and 20 patients with cirrhosis of the liver. HCV status was determined by the polymerase chain reaction. Plasma concentrations of metronidazole...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metronidazole; Liver function; Hepatitis C; Cytochrome P450.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300015

Nasal CPAP in the delivery room for newborns with extremely low birth weight in a hospital in a developing country
Provedor de dados: 56
Autores: Gonçalves-Ferri,W.A.; Martinez,F.E.
The objective of this study was to determine the feasibility of the use of continuous positive airway pressure installed prophylactically in the delivery room (DR-CPAP), for infants with a birth weight between 500 and 1000 g in settings with limited resources. During 23 months, infants with a birth weight between 500 and 1000 g consecutively received DR-CPAP. A total of 33 infants with low birth weight were enrolled, 16 (48.5%) were females. Only 14 (42.4%) received antenatal corticosteroids and only 2 of those 14 (14.3%) infants weighing 500-750 g were not intubated in the delivery room, and apnea was given as the reason for intubation of these patients. Of the 19 infants in the 751-1000 g weight range, 9 (47.4%) were intubated in the delivery room, 6 due...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Continuous positive airway pressure ventilation; Preterm infants; Delivery room; Nasal continuous positive airway pressure; Prematurity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000892

Publication in a Brazilian journal by Brazilian scientists whose papers have international impact
Provedor de dados: 56
Autores: Meneghini,R.
Nine Brazilian scientists with an outstanding profile of international publications were invited to publish an original article in the same issue of a Brazilian Journal (Anais da Academia Brasileira de Ciências). The objective was to measure the impact of the papers on the number of citations to the articles, the assumption being that these authors would carry their international prestige to the Brazilian periodical. In a 2-year period there was a larger number of citations of these articles compared to others published in the same journal. Nevertheless, the number of citations in Brazilian journals did not equal the number of citations obtained by the other papers by the same authors in their international publications within the same 2-year period. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Impact factor; Brazilian journals; Journal visibility.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900001

MTP -493G/T gene polymorphism is associated with steatosis in hepatitis C-infected patients
Provedor de dados: 56
Autores: Siqueira,E.R.F.; Oliveira,C.P.M.S.; Correa-Giannella,M.L.; Stefano,J.T.; Cavaleiro,A.M.; Fortes,M.A.H.Z.; Muniz,M.T.C.; Silva,F.S.; Pereira,L.M.M.B.; Carrilho,F.J.
The reduction of hepatic microsomal transfer protein (MTP) activity results in fatty liver, worsening hepatic steatosis and fibrosis in chronic hepatitis C (CHC). The G allele of the MTP gene promoter, -493G/T, has been associated with lower transcriptional activity than the T allele. We investigated this association with metabolic and histological variables in patients with CHC. A total of 174 untreated patients with CHC were genotyped for MTP -493G/T by direct sequencing using PCR. All patients were negative for markers of Wilson’s disease, hemochromatosis and autoimmune diseases and had current and past daily alcohol intake lower than 100 g/week. The sample distribution was in Hardy-Weinberg equilibrium. Among subjects with genotype 1, 56.8% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Steatosis; Fibrosis; Microsomal transfer protein.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100012

Inflammatory cytokines and plasma redox status responses in hypertensive subjects after heat exposure
Provedor de dados: 56
Autores: Fonseca,S.F.; Mendonça,V.A.; Teles,M.C.; Ribeiro,V.G.C.; Tossige-Gomes,R.; Neves,C.D.C.; Rocha-Vieira,E.; Leite,L.H.R.; Soares,D.D.; Coimbra,C.C.; Lacerda,A.C.R.
Hypertension is characterized by a pro-inflammatory status, including redox imbalance and increased levels of pro-inflammatory cytokines, which may be exacerbated after heat exposure. However, the effects of heat exposure, specifically in individuals with inflammatory chronic diseases such as hypertension, are complex and not well understood. This study compared the effects of heat exposure on plasma cytokine levels and redox status parameters in 8 hypertensive (H) and 8 normotensive (N) subjects (age: 46.5±1.3 and 45.6±1.4 years old, body mass index: 25.8±0.8 and 25.6±0.6 kg/m2, mean arterial pressure: 98.0±2.8 and 86.0±2.3 mmHg, respectively). They remained at rest in a sitting position for 10 min in a thermoneutral environment (22°C) followed by 30 min...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Heat; Cytokines; Redox status.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300701

Apolipoprotein B and angiotensin-converting enzyme polymorphisms and aerobic interval training: randomized controlled trial in coronary artery disease patients
Provedor de dados: 56
Autores: Tamburus,N.Y.; Verlengia,R.; Kunz,V.C.; César,M.C.; Silva,E.
Physical training has been strongly recommended as a non-pharmacological treatment for coronary artery disease (CAD). Genetic polymorphisms have been studied to understand the biological variability in response to exercise among individuals. This study aimed to verify the possible influence of apolipoprotein B (ApoB: rs1042031 and rs693) and angiotensin-converting enzyme (ACE-ID: rs1799752) genotypes on the lipid profile and functional aerobic capacity, respectively, after an aerobic interval training (AIT) program in patients with CAD and/or cardiovascular risk factors. Sixty-six men were randomized and assigned to trained group (n=32) or control group (n=34). Cardiopulmonary exercise test was performed to determine the ventilatory anaerobic threshold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical training; Lipids; Apolipoprotein B polymorphism; Angiotensin-converting enzyme polymorphism; Aerobic capacity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800601

Human T-cell lymphotropic virus type I (HTLV-I) proviral DNA viral load among asymptomatic patients and patients with HTLV-I-associated myelopathy/tropical spastic paraparesis
Provedor de dados: 56
Autores: Montanheiro,P.A.; Penalva de Oliveira,A.C.; Posada-Vergara,M.P.; Milagres,A.C.; Tauil,C.; Marchiori,P.E.; Duarte,A.J.S.; Casseb,J.
To evaluate the human T-cell lymphotropic virus type I (HTLV-I) proviral DNA load among asymptomatic HTLV-I-infected carriers and patients with HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), real time PCR using TaqMan probes for the pol gene was performed in two million peripheral blood mononuclear cells (PBMC). The albumin gene was the internal genomic control and MT2 cells were used as positive control. The results are reported as copies/10,000 PBMC, and the detection limit was 10 copies. A total of 89 subjects (44 HAM/TSP and 45 healthy HTLV-I-infected carriers) followed up at the Institute of Infectious Diseases "Emilio Ribas" and in the Neurology Division of Hospital of Clínicas were studied. The asymptomatic HTLV-I-infected...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HTLV-I; HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP); HTLV-I DNA viral load.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100011

Cross-talk between neurons and glia: highlights on soluble factors
Provedor de dados: 56
Autores: Gomes,F.C.A.; Spohr,T.C.L.S.; Martinez,R.; Moura Neto,V.
The development of the nervous system is guided by a balanced action between intrinsic factors represented by the genetic program and epigenetic factors characterized by cell-cell interactions which neural cells might perform throughout nervous system morphogenesis. Highly relevant among them are neuron-glia interactions. Several soluble factors secreted by either glial or neuronal cells have been implicated in the mutual influence these cells exert on each other. In this review, we will focus our attention on recent advances in the understanding of the role of glial and neuronal trophic factors in nervous system development. We will argue that the functional architecture of the brain depends on an intimate neuron-glia partnership.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuron-glia interaction; Trophic factors; Astrocyte; Neuron; Nervous system.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500008

Dipeptidyl peptidase IV (CD26) activity in the hematopoietic system: differences between the membrane-anchored and the released enzyme activity
Provedor de dados: 56
Autores: Pereira,D.A.; Gomes,L.; El-Cheikh,M.C.; Borojevic,R.
Dipeptidyl peptidase IV (DPP-IV; CD26) (EC 3.4.14.5) is a membrane-anchored ectoenzyme with N-terminal exopeptidase activity that preferentially cleaves X-Pro-dipeptides. It can also be spontaneously released to act in the extracellular environment or associated with the extracellular matrix. Many hematopoietic cytokines and chemokines contain DPP-IV-susceptible N-terminal sequences. We monitored DPP-IV expression and activity in murine bone marrow and liver stroma cells which sustain hematopoiesis, myeloid precursors, skin fibroblasts, and myoblasts. RT-PCR analysis showed that all these cells produced mRNA for DPP-IV. Partially purified protein reacted with a commercial antibody to CD26. The K M values for Gly-Pro-p-nitroanilide ranged from 0.43 to 0.98...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoiesis; Stroma; Exopeptidase; Ectoenzyme; Membrane rafts.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500003

Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection
Provedor de dados: 56
Autores: Corghi,D.B.; Gonçales,N.S.L.; Marques,S.B.D.; Gonçales Jr.,F.L.
Hepatitis C virus (HCV) infection is a global medical problem. The current standard of treatment consists of the combination of peginterferon plus ribavirin. This regimen eradicates HCV in 55% of cases. The immune response to HCV is an important determinant of disease evolution and can be influenced by various host factors. HLA class II may play an important role in immune response against HCV. The objective of the present study was to determine the distribution of HLA class II (DRB1 and DQB1) alleles, their association with chronic HCV infection and their response to interferon therapy. One hundred and two unrelated white Brazilian patients with chronic HCV infection, 52 responders (45 males and 7 females) and 50 non-responders (43 males and 7 females) to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA class II; HLA DR/DQ; HLA genotyping PCR; Hepatitis C virus; Chronic hepatitis C; Interferon treatment.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000009

Frequency of primary glomerular disease in northeastern China
Provedor de dados: 56
Autores: Wu,Yi-Qi; Wang,Zheng; Xu,Hua-Feng; Jin,Xiao-Ming; Zhou,Hai-Zhou.
Most frequently reported Chinese renal biopsy data have originated from southeastern China. The present study analyzed the renal biopsy data from northeastern China. The records of 1550 consecutive native patients who were diagnosed with primary glomerular diseases (PGD) after renal biopsy at our hospital during 2005-2009 were used. These patients were divided into four age groups for stratified analysis: &lt;15, 15-44, 45-59, and ≥60 years old. Among PGD, minimal change disease (MCD) was the most common histologically diagnosed disease (30.7%), followed by IgA nephropathy (IgAN), mesangial proliferative glomerulonephritis (MsPGN), membranous nephropathy (MN), membranoproliferative glomerulonephritis (MPGN), focal segmental glomerulosclerosis (FSGS), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primary glomerular disease; Renal biopsies; IgA nephropathy; China.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800011

Use of a cysteine proteinase from Carica candamarcensis as a protective agent during DNA extraction
Provedor de dados: 56
Autores: Genelhu,M.S.; Zanini,M.S.; Veloso,I.F.; Carneiro,A.M.D.; Lopes,M.T.P.; Salas,C.E.
We describe the use of a plant cysteine proteinase isolated from latex of Carica candamarcensis as a protective agent during isolation of bacterial DNA following growth in culture of these cells. Between 100 to 720 units of proteinase (1 µg = 6 units) afforded good DNA protection when incubated with various kinds of microorganisms. Agarose gel electrophoresis showed that the resulting DNA was similar in size to DNA preparations obtained by treatment with proteinase K. The viability of the resulting material was checked by PCR amplification using species-specific primers. After standing at room temperature (25oC) for 35 days, the enzyme lost 10% of its initial activity. The enzyme stability and good yield of DNA suggest the use of this proteinase as an...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cysteine proteinase; Proteinase K; C. candamarcensis; DNA extraction.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900005

Down-regulation of the cardiac sarcoplasmic reticulum ryanodine channel in severely food-restricted rats
Provedor de dados: 56
Autores: Vizotto,V.A.; Carvalho,R.F.; Sugizaki,M.M.; Lima,A.P.; Aragon,F.F.; Padovani,C.R.; Castro,A.V.B.; Dal Pai-Silva,M.; Nogueira,C.R.; Cicogna,A.C.
We have shown that myocardial dysfunction induced by food restriction is related to calcium handling. Although cardiac function is depressed in food-restricted animals, there is limited information about the molecular mechanisms that lead to this abnormality. The present study evaluated the effects of food restriction on calcium cycling, focusing on sarcoplasmic Ca2+-ATPase (SERCA2), phospholamban (PLB), and ryanodine channel (RYR2) mRNA expressions in rat myocardium. Male Wistar-Kyoto rats, 60 days old, were submitted to ad libitum feeding (control rats) or 50% diet restriction for 90 days. The levels of left ventricle SERCA2, PLB, and RYR2 were measured using semi-quantitative RT-PCR. Body and ventricular weights were reduced in 50% food-restricted...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Food restriction; SERCA2; Ryanodine channel; Phospholamban; MRNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100004

Intensive chemotherapy as salvage treatment for solid tumors: focus on germ cell cancer
Provedor de dados: 56
Autores: Selle,F.; Gligorov,J.; Richard,S.; Khalil,A.; Alexandre,I.; Avenin,D.; Provent,S.; Soares,D.G.; Lotz,J.P.
Germ cell tumors present contrasting biological and molecular features compared to many solid tumors, which may partially explain their unusual sensitivity to chemotherapy. Reduced DNA repair capacity and enhanced induction of apoptosis appear to be key factors in the sensitivity of germ cell tumors to cisplatin. Despite substantial cure rates, some patients relapse and subsequently die of their disease. Intensive doses of chemotherapy are used to counter mechanisms of drug resistance. So far, high-dose chemotherapy with hematopoietic stem cell support for solid tumors is used only in the setting of testicular germ cell tumors. In that indication, high-dose chemotherapy is given as the first or late salvage treatment for patients with either relapsed or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-dose chemotherapy; Germ cell tumors; Stem cell transplantation; Solid tumors; Breast cancer; Ovarian cancer.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100013

The RNA interference revolution
Provedor de dados: 56
Autores: Lenz,G.
The discovery of double-stranded RNA-mediated gene silencing has rapidly led to its use as a method of choice for blocking a gene, and has turned it into one of the most discussed topics in cell biology. Although still in its infancy, the field of RNA interference has already produced a vast array of results, mainly in Caenorhabditis elegans, but recently also in mammalian systems. Micro-RNAs are short hairpins of RNA capable of blocking translation, which are transcribed from genomic DNA and are implicated in several aspects from development to cell signaling. The present review discusses the main methods used for gene silencing in cell culture and animal models, including the selection of target sequences, delivery methods and strategies for a successful...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; SiRNA; MiRNA; Gene silencing; Knockdown.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200003

Advancement in liver laparoscopic resection – development of a new surgical device
Provedor de dados: 56
Autores: Vavra,P.; Karnik,L.; Skrobankova,M.; Jurcikova,J.; Ihnat,P.; Zonca,P.; Peteja,M.; El-Gendi,A.; Czudek,S.
Liver resection is the standard treatment for any liver lesion. Laparoscopic liver resection is associated with lower intra-operative blood loss and fewer complications than open resection. Access to the posterior part of the right liver lobe is very uncomfortable and difficult for surgeons due the anatomic position, especially when employing laparoscopic surgery. Based on these experiences, a new laparoscopic device was developed that is capable of bending its long axis and allowing the application of radiofrequency energy in areas that were not technically accessible. The device is equipped with four telescopic needle electrodes that cause tissue coagulation after the delivery of radiofrequency energy. Ex vivo testing was performed in 2012 and 2014 at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laparoscopic surgery; Liver; Radiofrequency energy; Posterior liver segments.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400617

Single-nucleotide polymorphism analysis of GH, GHR, and IGF-1 genes in minipigs
Provedor de dados: 56
Autores: Tian,Y.G.; Yue,M.; Gu,Y.; Gu,W.W.; Wang,Y.J.
Tibetan (TB) and Bama (BM) miniature pigs are two popular pig breeds that are used as experimental animals in China due to their small body size. Here, we analyzed single-nucleotide polymorphisms (SNPs) in gene fragments that are closely related to growth traits [growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor (IGF)-1)] in these pig breeds and a large white (LW) control pig breed. On the basis of the analysis of 100 BMs, 108 TBs, and 50 LWs, the polymorphic distribution levels of GH, GHR, and IGF-1 were significantly different among these three pig breeds. According to correlation analyses between SNPs and five growth traits - body weight (BW), body length (BL), withers height (WH), chest circumference (CC), and abdomen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minipigs; SNP; GH; GHR; IGF-1.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900753

Microglia and neurons in the hippocampus of migratory sandpipers
Provedor de dados: 56
Autores: Diniz,C.G.; Magalhães,N.G.M.; Sousa,A.A.; Santos Filho,C.; Diniz,D.G.; Lima,C.M.; Oliveira,M.A.; Paulo,D.C.; Pereira,P.D.C.; Sherry,D.F.; Picanço-Diniz,C.W.
The semipalmated sandpiper Calidris pusilla and the spotted sandpiper Actitis macularia are long- and short-distance migrants, respectively. C. pusilla breeds in the sub-arctic and mid-arctic tundra of Canada and Alaska and winters on the north and east coasts of South America. A. macularia breeds in a broad distribution across most of North America from the treeline to the southern United States. It winters in the southern United States, and Central and South America. The autumn migration route of C. pusilla includes a non-stop flight over the Atlantic Ocean, whereas autumn route of A. macularia is largely over land. Because of this difference in their migratory paths and the visuo-spatial recognition tasks involved, we hypothesized that hippocampal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hippocampus; Neurons; Microglia; Shorebirds; Stereology; Morphometry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100603

Variability in baseline laboratory measurements of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Provedor de dados: 56
Autores: Ladwig,R.; Vigo,A.; Fedeli,L.M.G.; Chambless,L.E.; Bensenor,I.; Schmidt,M.I.; Vidigal,P.G.; Castilhos,C.D.; Duncan,B.B.
Multi-center epidemiological studies must ascertain that their measurements are accurate and reliable. For laboratory measurements, reliability can be assessed through investigation of reproducibility of measurements in the same individual. In this paper, we present results from the quality control analysis of the baseline laboratory measurements from the ELSA-Brasil study. The study enrolled 15,105 civil servants at 6 research centers in 3 regions of Brazil between 2008–2010, with multiple biochemical analytes being measured at a central laboratory. Quality control was ascertained through standard laboratory evaluation of intra- and inter-assay variability and test-retest analysis in a subset of randomly chosen participants. An additional sample of urine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multi-center studies; Laboratory measurements; Quality control.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900701

MMP-1/PAR-1 signal transduction axis and its prognostic impact in esophageal squamous cell carcinoma
Provedor de dados: 56
Autores: Peng,Hong-hua; Zhang,Xi; Cao,Pei-guo.
The matrix metalloprotease-1 (MMP-1)/protease-activated receptor-1 (PAR-1) signal transduction axis plays an important role in tumorigenesis. To explore the expression and prognostic value of MMP-1 and PAR-1 in esophageal squamous cell carcinoma (ESCC), we evaluated the expression of two proteins in resected specimens from 85 patients with ESCC by immunohistochemistry. Sixty-two (72.9%) and 58 (68.2%) tumors were MMP-1- and PAR-1-positive, respectively, while no significant staining was observed in normal esophageal squamous epithelium. MMP-1 and PAR-1 overexpression was significantly associated with tumor node metastasis (TNM) stage and regional lymph node involvement. Patients with MMP-1- and PAR-1-positive tumors, respectively, had poorer disease-free...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix metalloprotease-1; Protease-activated receptor-1; Esophageal squamous cell carcinoma; Prognosis; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100014

Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
Provedor de dados: 56
Autores: Diniz,S.F.; Amorim,F.P.L.G.; Cavalcante-Neto,F.F.; Bocca,A.L.; Batista,A.C.; Simm,G.E.P.M.; Silva,T.A.
A closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alloxan; Diabetes; Fracture repair; Closed fracture.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500005

Modulation of store-operated Ca2+ entry by cyclic-ADP-ribose
Provedor de dados: 56
Autores: Thompson,M.; White,T.; Chini,E.N.
Store-operated Ca2+ entry plays an important role in Ca2+ homeostasis in cells but the mechanisms of control of these channels are not completely understood. We describe an investigation of the role of the CD38-cyclic-ADP-ribose (cADPR)-ryanodine-channel (RyR) signaling pathway in store-operated Ca2+ entry in human smooth muscle. We observed that human myometrial cells have a functional store-operated Ca2+ entry mechanism. Furthermore, we observed the presence of transient receptor potential 1, 3, 4, 5, and 6 ion channels in human myometrial cells. Store-operated Ca2+ transient was inhibited by at least 50-70% by several inhibitors of the RyR, including ryanodine (10 µM), dantrolene (10 µM), and ruthenium red (10 µM). Furthermore, the cell permeable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclic-ADP-ribose; IP3; Endoplasmic reticulum; Ryanodine channel; Calcium entry; Human smooth muscle.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600006

Interregional correlations of glucose metabolism between the basal ganglia and different cortical areas: an ultra-high resolution PET/MRI fusion study using 18F-FDG
Provedor de dados: 56
Autores: Kim,J.H.; Son,Y.D.; Kim,J.M.; Kim,H.K.; Kim,Y.B.; Lee,C.; Oh,C.H.
Basal ganglia have complex functional connections with the cerebral cortex and are involved in motor control, executive functions of the forebrain, such as the planning of movement, and cognitive behaviors based on their connections. The aim of this study was to provide detailed functional correlation patterns between the basal ganglia and cerebral cortex by conducting an interregional correlation analysis of the 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) data based on precise structural information. Fifteen participants were scanned with 7-Tesla magnetic resonance imaging (MRI) and high resolution research tomography (HRRT)-PET fusion system using 18F-FDG. For detailed interregional correlation analysis, 24 subregions of the basal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basal ganglia; 18F-FDG PET/MRI; ROI-based interregional correlation analysis; Functional correlation; Glucose metabolism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100613

Snake venom metalloproteinases and disintegrins: interactions with cells
Provedor de dados: 56
Autores: Kamiguti,A.S.; Zuzel,M.; Theakston,R.D.G.
Metalloproteinases and disintegrins are important components of most viperid and crotalid venoms. Large metalloproteinases referred to as MDC enzymes are composed of an N-terminal Metalloproteinase domain, a Disintegrin-like domain and a Cys-rich C-terminus. In contrast, disintegrins are small non-enzymatic RGD-containing cysteine-rich polypeptides. However, the disintegrin region of MDC enzymes bears a high degree of structural homology to that of the disintegrins, although it lacks the RGD motif. Despite these differences, both components share the property of being able to recognize integrin cell surface receptors and thereby to inhibit integrin-dependent cell reactions. Recently, several membrane-bound MDC enzymes, closely related to soluble venom MDC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Metalloproteinase; Disintegrin; Platelet; Integrin; Signaling.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700001

Evaluation of the cytotoxic and genotoxic effects by melamine and cyanuric acid co-exposure in human embryonic kidney 293 cells
Provedor de dados: 56
Autores: Xu,Xianrong; Lu,Jing; Sheng,Hongqiang; Zhang,Long; Gan,Tieer; Zhang,Jianyun; Xu,Yuying; Zhu,Xinqiang; Yang,Jun.
The melamine and cyanuric acid (CA) complex has been suggested to cause the toxic effects observed in melamine-contaminated food or milk. However, the cytotoxic and genotoxic effects of co-exposure to melamine and CA are not fully clear. Therefore, the cytotoxic effects of melamine and CA were first examined by co‐exposure in human kidney 293 cells using the MTT assay. During a 24-h period for the three concentrations tested (0.5, 1, and 5 mg/mL), neither melamine nor CA alone showed significant toxic effects on 293 cells at 0.5 mg/mL, while higher concentrations led to decreased in cell viability. However, co-exposure to several combinations of melamine and CA [100:1, 10:1, 1:10, and 1:100 (v:v), at a final concentration of 0.5 mg/mL] did cause...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melamine; Cyanuric acid; ΓH2AX; DNA damage; Genotoxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500612

Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3′-untranslated region
Provedor de dados: 56
Autores: Piranda,D.N.; Abreu,R.B.V.; Freitas-Alves,D.R.; de Carvalho,M.A.; Vianna-Jorge,R.
The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′-UTR). Different PR (rs689465, rs689466, rs20417) and 3′-UTR (rs5275) variants were generated by site-directed mutagenesis, and combined in haplotypes to access expression levels using a reporter system (luciferase) in human cells (MCF-7 and HEK293FT). Luciferase activity did not differ significantly among PTGS2 PR constructs, except for pAAC (containing variant allele rs20417 C), with 40% less activity than pAAG (wild-type sequence) in MCF-7 cells (P&lt;0.01). Despite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COX-2; PTGS2; Haplotypes; Gene expression; MCF-7; HEK293FT.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200602

Fluorescent quantitative PCR of Mycobacterium tuberculosis for differentiating intestinal tuberculosis from Crohn's disease
Provedor de dados: 56
Autores: Fei,B.Y.; Lv,H.X.; Zheng,W.H.
Intestinal tuberculosis (ITB) and Crohn's disease (CD) are granulomatous disorders with similar clinical manifestations and pathological features that are often difficult to differentiate. This study evaluated the value of fluorescent quantitative polymerase chain reaction (FQ-PCR) for Mycobacterium tuberculosis (MTB) in fecal samples and biopsy specimens to differentiate ITB from CD. From June 2010 to March 2013, 86 consecutive patients (38 females and 48 males, median age 31.3 years) with provisional diagnoses of ITB and CD were recruited for the study. The patients' clinical, endoscopic, and histological features were monitored until the final definite diagnoses were made. DNA was extracted from 250 mg fecal samples and biopsy tissues from each patient....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal tuberculosis; Crohn's disease; Diagnosis; Fluorescent quantitative PCR; Mycobacterium tuberculosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200166

Induction of Zenk protein expression within the nucleus taeniae of the amygdala of pigeons following tone and shock stimulation
Provedor de dados: 56
Autores: Brito,I.; Britto,L.R.G.; Ferrari,E.A.M.
In this study, we evaluated the expression of the Zenk protein within the nucleus taeniae of the pigeon’s amygdala (TnA) after training in a classical aversive conditioning, in order to improve our understanding of its functional role in birds. Thirty-two 18-month-old adult male pigeons (Columba livia), weighing on average 350 g, were trained under different conditions: with tone-shock associations (experimental group; EG); with shock-alone presentations (shock group; SG); with tone-alone presentations (tone group; TG); with exposure to the training chamber without stimulation (context group; CG), and with daily handling (naive group; NG). The number of immunoreactive nuclei was counted in the whole TnA region and is reported as density of Zenk-positive...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Amygdala; Nucleus taeniae of the amygdala; Fear conditioning; Zenk; Pigeon.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800005

Epigallocatechin-3-gallate ameliorates lipopolysaccharide-induced acute lung injury by suppression of TLR4/NF-κB signaling activation
Provedor de dados: 56
Autores: Wang,Jia; Fan,Shi Ming; Zhang,Jiong.
Acute lung injury (ALI) is a serious clinical syndrome with a high rate of mortality. The activation of inflammation is well-recognized as a vital factor in the pathogenesis of lipopolysaccharide (LPS)-induced ALI. Therefore, suppression of the inflammatory response could be an ideal strategy to prevent ALI. Epigallocatechin-3-gallate (EGCG), mainly from green tea, has been shown to have an anti-inflammatory effect. The aim of the study was to explore whether EGCG alleviates inflammation in sepsis-related ALI. Male BALB/C mice were treated with EGCG (10 mg/kg) intraperitoneally (ip) 1 h before LPS injection (10 mg/kg, ip). The results showed that EGCG attenuated LPS-induced ALI as it decreased the changes in blood gases and reduced the histological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigallocatechin-3-gallate; Acute lung injury; Inflammation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700605

Chronic granulomatous disease: why an inflammatory disease?
Provedor de dados: 56
Autores: Roxo-Junior,P.; Simão,H.M.L.
Chronic granulomatous disease is a primary immunodeficiency caused by mutations in the genes encoding subunits of the phagocytic NADPH oxidase system. Patients can present with severe, recurrent infections and noninfectious conditions. Among the latter, inflammatory manifestations are predominant, especially granulomas and colitis. In this article, we systematically review the possible mechanisms of hyperinflammation in this rare primary immunodeficiency condition and their correlations with clinical aspects.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic granulomatous disease; Inflammation; Infection; Granulomas; NADPH oxidase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100924

A spheroid-based 3-D culture model for pancreatic cancer drug testing, using the acid phosphatase assay
Provedor de dados: 56
Autores: Wen,Z.; Liao,Q.; Hu,Y.; You,L.; Zhou,L.; Zhao,Y.
Current therapy for pancreatic cancer is multimodal, involving surgery and chemotherapy. However, development of pancreatic cancer therapies requires a thorough evaluation of drug efficacy in vitro before animal testing and subsequent clinical trials. Compared to two-dimensional culture of cell monolayer, three-dimensional (3-D) models more closely mimic native tissues, since the tumor microenvironment established in 3-D models often plays a significant role in cancer progression and cellular responses to the drugs. Accumulating evidence has highlighted the benefits of 3-D in vitro models of various cancers. In the present study, we have developed a spheroid-based, 3-D culture of pancreatic cancer cell lines MIAPaCa-2 and PANC-1 for pancreatic drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spheroid; Three-dimensional culture; Pancreatic cancer; Drug testing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700634

Tai Chi versus routine exercise in patients with early- or mild-stage Parkinson's disease: a retrospective cohort analysis
Provedor de dados: 56
Autores: Li,Quanhao; Liu,Jinmei; Dai,Fei; Dai,Fengling.
Parkinson's disease cannot be cured but symptoms can be improved by making use of physical therapy. The objective of the study was to compare the effect of routine exercises and Tai Chi on physical and clinical performance in elderly people suffering from Parkinson's disease. Data from interviews, physical and clinical performance, and levodopa consumption of 500 patients with confirmed Parkinson's disease (severity level I to III) were collected and analyzed. Participants who received 80 min/day Tai Chi 3 times/week for 2 months were included in the Tai Chi (TC) group (n=250) and those who received 90 min/day routine exercise 3 times/week for 2 months were included in routine exercise (RE) group (n=250). Timed up-and-go, 50-foot speed walk, and functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Fall; Levodopa; Parkinson's disease; Physical therapy; Tai Chi.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200606

A dose-response curve for biodosimetry from a 6 MV electron linear accelerator
Provedor de dados: 56
Autores: Lemos-Pinto,M.M.P.; Cadena,M.; Santos,N.; Fernandes,T.S.; Borges,E.; Amaral,A.
Biological dosimetry (biodosimetry) is based on the investigation of radiation-induced biological effects (biomarkers), mainly dicentric chromosomes, in order to correlate them with radiation dose. To interpret the dicentric score in terms of absorbed dose, a calibration curve is needed. Each curve should be constructed with respect to basic physical parameters, such as the type of ionizing radiation characterized by low or high linear energy transfer (LET) and dose rate. This study was designed to obtain dose calibration curves by scoring of dicentric chromosomes in peripheral blood lymphocytes irradiated in vitro with a 6 MV electron linear accelerator (Mevatron M, Siemens, USA). Two software programs, CABAS (Chromosomal Aberration Calculation Software)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dicentrics; Calibration curves; Linear accelerator; CABAS; Dose Estimate.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000008

A double-blind comparison of the effect of the antipsychotics haloperidol and olanzapine on sleep in mania
Provedor de dados: 56
Autores: Moreno,R.A.; Hanna,M.M.; Tavares,S.M.; Wang,Y .P.
The effects of haloperidol and olanzapine on polysomnographic measures made in bipolar patients during manic episodes were compared. Twelve DSM-IV mania patients were randomly assigned to receive either haloperidol (mean ± SD final dosage: 5.8 ± 3.8 mg) or olanzapine (mean ± SD final dosage: 13.6 ± 6.9 mg) in a 6-week, double-blind, randomized, controlled clinical trial. One-night polysomnographic evaluation was performed before and after the haloperidol or olanzapine treatment. Psychopathology and illness severity were rated respectively with the Young Mania Rating Scale (YMRS) and the Clinical Global Impressions - Bipolar version (CGI-BP). There was a significant improvement in the YMRS and CGI-BP scores at the end of the study for both groups. Mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olanzapine; Haloperidol; Sleep; Mania; Bipolar disorder; Polysomnographic measurements.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300011

Extracts of Azadirachta indica and Melia azedarach seeds inhibit folliculogenesis in albino rats
Provedor de dados: 56
Autores: Roop,J.K.; Dhaliwal,P.K.; Guraya,S.S.
The seed oil of Azadirachta indica A. Juss (neem) is used in traditional medicine for its antidiabetic, spermicidal, antifertility, antibacterial, and wound healing properties. The present study was undertaken to investigate the quantitative aspects of follicular development in cyclic female albino rats (135 ± 10 g; 8 groups with 6 animals in each group) after oral administration of polar (PF) and non-polar (NPF) fractions of A. indica seed extract at 3 and 6 mg kg body weight-1 day-1 and Melia azedarach Linn. (dharek) seed extract at 24 mg kg body weight-1 day-1 for 18 days. The extracts were prepared using a flash evaporator at 35°C and then dissolved in olive oil to prepare doses on a per kg body weight basis. There was a significant reduction (P =...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Folliculogenesis; Azadirachta; Melia; Plant products; Antifertility.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600017

Effects of type of physical exercise and leisure activities on the depression scores of obese Brazilian adolescent girls
Provedor de dados: 56
Autores: Stella,S.G.; Vilar,A.P.; Lacroix,C.; Fisberg,M.; Santos,R.F.; Mello,M.T.; Tufik,S.
Several studies have indicated that depressive states may lead to hypokinesia with diminished metabolic rate and energy use. Hypokinesia associated with certain eating behaviors may lead to an unfavorable energy balance that can contribute to the emergence and prevalence of obesity among children and adults. The purpose of the present study was to examine the possibility of reducing depression inventory scores in female adolescents with third-degree obesity while testing the effectiveness of different exercise programs in reducing anxiety and depression scores. The sample consisted of 40 female subjects (mean age 16 ± 1.56 years) divided into 4 groups (aerobic training, anaerobic training, leisure activities, and control). Subjects had a body mass index of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adolescents; Physical exercise; Leisure activity; Obesity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100017

The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain
Provedor de dados: 56
Autores: Pejic,S.; Kasapovic,J.; Cvetkovic,D.; Pajovic,S.B.
The sensitivity of copper,zinc (CuZn)- and manganese (Mn)-superoxide dismutase (SOD) to exogenous estradiol benzoate (EB) was investigated in Wistar rats during postnatal brain development. Enzyme activities were measured in samples prepared from brains of rats of both sexes and various ages between 0 and 75 days, treated sc with 0.5 µg EB/100 g body weight in 0.1 ml olive oil/100 g body weight, 48 and 24 h before sacrifice. In females, EB treatment stimulated MnSOD activity on days 0 (66.1%), 8 (72.7%) and 15 (81.7%). In males, the stimulatory effect of EB on MnSOD activity on day 0 (113.6%) disappeared on day 8 and on days 15 and 45 it became inhibitory (40.3 and 30.5%, respectively). EB had no effect on the other age groups. The stimulatory effect of EB...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Brain development; Estradiol; Superoxide dismutase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500004

Adipose tissue-derived stem cells promote pancreatic cancer cell proliferation and invasion
Provedor de dados: 56
Autores: Ji,S.Q.; Cao,J.; Zhang,Q.Y.; Li,Y.Y.; Yan,Y.Q.; Yu,F.X.
To explore the effects of adipose tissue-derived stem cells (ADSCs) on the proliferation and invasion of pancreatic cancer cells in vitroand the possible mechanism involved, ADSCs were cocultured with pancreatic cancer cells, and a cell counting kit (CCK-8) was used to detect the proliferation of pancreatic cancer cells. ELISA was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. RT-PCR was performed to detect the expression of the chemokine receptor CXCR4 in pancreatic cancer cells and ADSCs. An in vitro invasion assay was used to measure invasion of pancreatic cancer cells. SDF-1 was detected in the supernatants of ADSCs, but not in pancreatic cancer cells. Higher CXCR4 mRNA levels were detected in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipose tissue-derived stem cells; Pancreatic cancer; Proliferation; Invasion; SDF-1.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900758

Validation of the Beck Depression Inventory for a Portuguese-speaking Chinese community in Brazil
Provedor de dados: 56
Autores: Wang,Y.-P.; Andrade,L.H.; Gorenstein,C.
The objective of the present study was to investigate the psychometric properties and cross-cultural validity of the Beck Depression Inventory (BDI) among ethnic Chinese living in the city of São Paulo, Brazil. The study was conducted on 208 community individuals. Reliability and discriminant analysis were used to test the psychometric properties and validity of the BDI. Principal component analysis was performed to assess the BDI's factor structure for the total sample and by gender. The mean BDI score was lower (6.74, SD = 5.98) than observed in Western counterparts and showed no gender difference, good internal consistency (Cronbach's alpha 0.82), and high discrimination of depressive symptoms (75-100%). Factor analysis extracted two factors for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depressive symptoms; Beck Depression Inventory; Psychometric properties; Validation of Depression Inventory; Portuguese-speaking Chinese; Factor analysis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300011

Distribution of microglial cells in the cerebral hemispheres of embryonic and neonatal chicks
Provedor de dados: 56
Autores: Ignácio,A.R.; Müller,Y.M.R.; Carvalho,M.S.L.; Nazari,E.M.
The distribution, morphology and morphometry of microglial cells in the chick cerebral hemispheres from embryonic day 4 (E4) to the first neonatal day (P1) were studied by histochemical labeling with a tomato (Lycopersicon esculentum) lectin. The histochemical analysis revealed lectin-reactive cells in the nervous parenchyma on day E4. Between E4 (5.7 ± 1.35 mm length) and E17 (8.25 ± 1.2 mm length), the lectin-reactive cells were identified as ameboid microglia and observed starting from the subventricular layer, distributed throughout the mantle layer and in the proximity of the blood vessels. After day E13, the lectin-reactive cells exhibited elongated forms with small branched processes, and were considered primitive ramified microglia. Later, between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microglia; Cerebral hemispheres; Embryos; Neonatal chicks; Gallus domesticus; Tomato lectin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100008

Coexistence of potentiation and fatigue in skeletal muscle
Provedor de dados: 56
Autores: Rassier,D.E.; MacIntosh,B.R.
Twitch potentiation and fatigue in skeletal muscle are two conditions in which force production is affected by the stimulation history. Twitch potentiation is the increase in the twitch active force observed after a tetanic contraction or during and following low-frequency stimulation. There is evidence that the mechanism responsible for potentiation is phosphorylation of the regulatory light chains of myosin, a Ca2+-dependent process. Fatigue is the force decrease observed after a period of repeated muscle stimulation. Fatigue has also been associated with a Ca2+-related mechanism: decreased peak Ca2+ concentration in the myoplasm is observed during fatigue. This decrease is probably due to an inhibition of Ca2+ release from the sarcoplasmic reticulum....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Posttetanic potentiation; Staircase; High-frequency fatigue; Low-frequency fatigue; Ca2+ sensitivity; RLC phosphorylation; Myosin light chains.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500003

Transforming growth factor beta activity in urine of patients with type 2 diabetes and diabetic nephropathy
Provedor de dados: 56
Autores: Rivarola,E.W.R.; Moyses-Neto,M.; Dantas,M.; da-Silva,C.G.; Volpini,R.; Coimbra,T.M.
Diabetic nephropathy (DN) is characterized structurally by progressive mesangial deposition of extracellular matrix (ECM). Transforming growth factor-ß (TGF-ß) is considered to be one of the major cytokines involved in the regulation of ECM synthesis and degradation. Several studies suggest that an increase in urinary TGF-ß levels may reflect an enhanced production of this polypeptide by the kidney cells. We evaluated TGF-ß in occasional urine samples from 14 normal individuals and 23 patients with type 2 diabetes (13 with persistent proteinuria &amp;gt;500 mg/24 h, DN, 6 with microalbuminuria, DMMA, and 4 with normal urinary albumin excretion, DMN) by enzyme immunoassay. An increase in the rate of urinary TGF-ß excretion (pg/mg UCreat.) was observed in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: TGF-ß urinary excretion; Type 2 diabetes; Proteinuria; Diabetic nephropathy; Extracellular matrix.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200011

Differential effect of plant lectins on mast cells of different origins
Provedor de dados: 56
Autores: Lopes,F.C.; Cavada,B.S.; Pinto,V.P.T.; Sampaio,A.H.; Gomes,J.C.
Histamine release induced by plant lectins was studied with emphasis on the carbohydrate specificity, external calcium requirement, metal binding sites, and mast cell heterogeneity and on the importance of antibodies bound to the mast cell membrane to the lectin effect. Peritoneal mast cells were obtained by direct lavage of the rat peritoneal cavity and guinea pig intestine and hamster cheek pouch mast cells were obtained by dispersion with collagenase type IA. Histamine release was induced with concanavalin A (Con A), lectins from Canavalia brasiliensis, mannose-specific Cymbosema roseum, Maackia amurensis, Parkia platycephala, Triticum vulgaris (WGA), and demetallized Con A and C. brasiliensis, using 1-300 µg/ml lectin concentrations applied to Wistar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mast cells; Histamine; Plant lectins.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600016

Superoxide: a two-edged sword
Provedor de dados: 56
Superoxide (O2-) is the compound obtained when oxygen is reduced by one electron. For a molecule with an unpaired electron, O2- is surprisingly inert, its chief reaction being a dismutation in which it reacts with itself to form H2O2 and oxygen. The involvement of O2- in biological systems was first revealed by the discovery in 1969 of superoxide dismutase, an enzyme that catalyzes the dismutation of O2-. Since then it has been found that biological systems produce a bewildering variety of reactive oxidants, all but a few arising ultimately from O2-. These oxidants include O2- itself, H2O2 and alkyl peroxides, hydroxyl radical and other reactive oxidizing radicals, oxidized halogens and halamines, singlet oxygen, and peroxynitrite. These various oxidants...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: NADPH oxidase; Superoxide; Oxidative stress; Antioxidant; Regulation; Host defense.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200001

Relationship between microalbuminuria and cardiac structural changes in mild hypertensive patients
Provedor de dados: 56
Autores: Plavnik,F.L.; Silva,M.A.M.R.T.; Kohlmann,N.E.B.; Kohlmann Jr.,O.; Ribeiro,A.B.; Zanella,M.T.
The aim of this study was to determine the relationship between urinary albumin excretion (UAE), cardiac structural changes upon echocardiography and 24-h ambulatory blood pressure (ABPM) levels. Twenty mild hypertensive patients (mean age 56.8 ± 9.6 years) were evaluated. After 2 weeks of a washout period of all antihypertensive drugs, all patients underwent an echocardiographic evaluation, a 24-h ABPM and an overnight urine collection. Systolic and diastolic blood pressure during 24-h ABPM was 145 ± 14/91 ± 10 mmHg (daytime) and 130 ± 14/76 ± 8 mmHg (nighttime), respectively. Seven (35%) patients presented UAE  &gt; or = 15 µg/min, and for the whole group, the geometric mean value for UAE was 10.2 x/÷ 3.86 µg/min. Cardiac measurements showed mean values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Microalbuminuria; Left ventricular hypertrophy; Blood pressure monitoring.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700006

Decreased platelet responsiveness to clopidogrel correlates with CYP2C19 and PON1 polymorphisms in atherosclerotic patients
Provedor de dados: 56
Autores: Marchini,J.F.M.; Pinto,M.R.; Novaes,G.C.; Badran,A.V.; Pavão,R.B.; Figueiredo,G.L.; Lago,I.M.; Lima-Filho,M.O.; Lemos,D.C.; Tonani,M.; Antloga,C.M.; Oliveira,L.; Lorenzi,J.C.; Marin-Neto,J.A.
Clopidogrel and aspirin are the most commonly used medications worldwide for dual antiplatelet therapy after percutaneous coronary intervention. However, clopidogrel hyporesponsiveness related to gene polymorphisms is a concern. Populations with higher degrees of genetic admixture may have increased prevalence of clopidogrel hyporesponsiveness. To assess this, we genotyped CYP2C19, ABCB1, and PON1 in 187 patients who underwent percutaneous coronary intervention. Race was self-defined by patients. We also performed light transmission aggregometry with adenosine diphosphate (ADP) and arachidonic acid during dual antiplatelet therapy. We found a significant difference for presence of the CYP2C19*2 polymorphism between white and non-white patients. Although 7%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Platelet function tests; Single nucleotide polymorphism; Percutaneous coronary intervention; Aspirin; Clopidogrel.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100702

Effects of chronic corticosterone and imipramine administration on panic and anxiety-related responses
Provedor de dados: 56
Autores: Diniz,L.; dos Reis,B.B.; de Castro,G.M.; Medalha,C.C.; Viana,M.B.
It is known that chronic high levels of corticosterone (CORT) enhance aversive responses such as avoidance and contextual freezing. In contrast, chronic CORT does not alter defensive behavior induced by the exposure to a predator odor. Since different defense-related responses have been associated with specific anxiety disorders found in clinical settings, the observation that chronic CORT alters some defensive behaviors but not others might be relevant to the understanding of the neurobiology of anxiety. In the present study, we investigated the effects of chronic CORT administration (through surgical implantation of a 21-day release 200 mg pellet) on avoidance acquisition and escape expression by male Wistar rats (200 g in weight at the beginning of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Corticosterone; Imipramine; Generalized anxiety disorder; Panic disorder; Elevated T-maze.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000010

The effect of microgravity on tissue structure and function of rat testis
Provedor de dados: 56
Autores: Ding,Ye; Tang,Jin; Zou,Jun; She,Ruiping; Wang,Yinghua; Yue,Zhuo; Tian,Jijing; Xia,Kangkang; Yin,Jun; Wang,Desheng.
To explore whether an environment of weightlessness will cause damage to the reproductive system of animals, we used the tail-suspension model to simulate microgravity, and investigated the effect of microgravity on the tissue structure and function of the testis in sexually mature male rats. Forty-eight male Wistar rats weighing 200-250 g were randomly assigned to three groups (N = 16 each): control, tail traction, and tail suspension. After the rats were suspended for 7 or 14 days, morphological changes of testis were evaluated by histological and electron microscopic methods. The expression of HSP70, bax/bcl-2 and AR (androgen receptor) in testis was measured by immunohistochemistry. Obvious pathological lesions were present in the testis after the rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simulated microgravity; Histopathology; AR; HSP70; Bax/bcl-2; Testis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200007

Effect of bisacodyl on rats with slow transit constipation
Provedor de dados: 56
Autores: Wang,Yong-bing; Ling,Jie; Zhang,Wen-zhong; Li,Gang; Qiu,Wei; Zheng,Jun-hua; Zhao,Xiao-hui.
The effect of bisacodyl on the treatment of rats with slow transit constipation (STC) was studied. Forty-five female Wister rats were divided into control group, STC group, and STC bisacodyl group. The immunohistochemical method was used to determine interstitial cells of Cajal (ICC) and the expression of c-Kit protein. Body mass and the number of defecations were significantly decreased in the STC group compared with the control group on the 100th day after diphenoxylate administration, while dry weight of feces was significantly increased and the intestinal transit time was prolonged. There were significant differences in the number of defecations, dry weight of feces, and intestinal transit time among the three groups. The number of defecations was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bisacodyl; Slow transit constipation; C-Kit.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700613

Immunohistochemical demonstration of TGF-ß and decorin in paracoccidioidal granulomas
Provedor de dados: 56
Autores: Nishikaku,A.S.; Burger,E.
Different patterns of granulomas have been observed in 6- to 8-week-old mice after ip inoculation with 5 x 10(6) yeast cells of Paracoccidioides brasiliensis. Transforming growth factor-ß (TGF-ß) is a cytokine that has been shown to participate in fibrosis and granuloma formation; its activities seem to be modulated by the small proteoglycan decorin. In the present study, TGF-ß and decorin expression in epiploon granulomas was assessed by immunohistochemistry in susceptible (B10.A) and resistant (A/J) mice after 15, 30, 120 and 150 days of P. brasiliensis ip infection. The epiploon was collected, fixed in Methacarn solution and embedded in paraffin, and 5-µm thick sections were used for immunohistochemical analysis employing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TGF-ß; Decorin; Paracoccidioides brasiliensis; Granulomatous lesions; Susceptibility and resistance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800014

Coronary endothelial dysfunction after ischemia and reperfusion: a new therapeutic target?
Provedor de dados: 56
Autores: Laude,K.; Thuillez,C.; Richard,V.
Although cardiac ischemia is usually characterized as a disease of the myocyte, it is clear that the vasculature, and especially endothelial cells, is also a major target of this pathology. Indeed, using a rat model of ischemia/reperfusion, we were able to detect severe endothelial dysfunction (assessed as a decreased response to acetylcholine) after acute or chronic reperfusion. Given the essential role of the endothelium in the regulation of vascular tone, as well as platelet and leukocyte function, such a severe dysfunction could lead to an increased risk of vasospasm, thrombosis and accelerated atherosclerosis. This dysfunction can be prevented by free radical scavengers and by exogenous nitric oxide. Endothelial dysfunction can also be prevented by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary endothelium; Ischemia/reperfusion; Preconditioning.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100001

Validation of the Brazilian Children's Test of Pseudoword Repetition in Portuguese speakers aged 4 to 10 years
Provedor de dados: 56
Autores: Santos,F.H.; Bueno,O.F.A.
The phonological loop is a component of the working memory system specifically involved in the processing and manipulation of limited amounts of information of a sound-based phonological nature. Phonological memory can be assessed by the Children's Test of Nonword Repetition (CNRep) in English speakers but not in Portuguese speakers due to phonotactic differences between the two languages. The objectives of the present study were: 1) to develop the Brazilian Children's Test of Pseudoword Repetition (BCPR), a Portuguese version of the CNRep, and 2) to validate the BCPR by correlation with the Auditory Digit Span Test from the Stanford-Binet Intelligence Scale. The BCPR and Digit Span were assessed in 182 children aged 4-10 years, 84 from Minas Gerais State...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Working memory; Children's Test of Nonword Repetition; Pseudoword repetition test; Phonological loop; Portuguese language.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100012

Cholesterol-dependent hemolytic activity of Passiflora quadrangularis leaves
Provedor de dados: 56
Autores: Yuldasheva,L.N.; Carvalho,E.B.; Catanho,M.-T.J.A.; Krasilnikov,O.V.
Plants used in traditional medicine are rich sources of hemolysins and cytolysins, which are potential bactericidal and anticancer drugs. The present study demonstrates for the first time the presence of a hemolysin in the leaves of Passiflora quadrangularis L. This hemolysin is heat stable, resistant to trypsin treatment, has the capacity to froth, and acts very rapidly. The hemolysin activity is dose-dependent, with a slope greater than 1 in a double-logarithmic plot. Polyethylene glycols of high molecular weight were able to reduce the rate of hemolysis, while liposomes containing cholesterol completely inhibited it. In contrast, liposomes containing phosphatidylcholine were ineffective. The Passiflora hemolysin markedly increased the conductance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora quadrangularis; Leaf extract; Liposomes; Bilayer permeation; Hemolysin; Membrane cholesterol.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700009

Behavioral modulation by mutilation pictures in women
Provedor de dados: 56
Autores: Pereira,M.G.; Volchan,E.; Oliveira,L.; Machado-Pinheiro,W.; Rodrigues,J.A.; Nepomuceno,F.V.P.; Pessoa,L.
Previous studies have shown that women are more emotionally expressive than men. It is unclear, however, if women are also more susceptible to the emotional modulation of behavior imposed by an affective stimulus. To investigate this issue, we devised a task in which female subjects performed six sequential trials of visual target detection following the presentation of emotional (mutilation and erotic) or neutral pictures (domestic utensils and objects) and compared the data obtained in the present study with those described in a previous study with male subjects. The experiment consisted of three blocks of 24 pictures and each block had an approximate duration of 4 min. Our sample consisted of 36 subjects (age range: 18 to 26 years) and each subject...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emotion; Behavior; Reaction time; Sex differences; Affective stimulus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300011

Inhibition of cyclooxygenase activity by standardized hydroalcoholic extracts of four Asteraceae species from the Argentine Puna
Provedor de dados: 56
Autores: Alberto,M.R.; Zampini,I.C.; Isla,M.I.
We determined the anti-inflammatory activity of standardized extracts of four medicinal plant species (Baccharis incarum, B. boliviensis, Chuquiraga atacamensis, Parastrephia lucida) that grow in the Argentine Puna (3800 m above sea level) and that are used to reduce oxidative stress and alleviate gout and arthritic pain. The extracts of plant aerial parts were standardized in terms of total phenolic compounds and flavone/flavanone content and free radical scavenging activity. All extracts showed high phenolic compound concentration (0.5-1.6 mg/mL), mainly flavones and flavonols (0.1-0.8 mg/mL). The extracts showed hydrogen donating ability (DPPH and ABTS) and reactive oxygen species scavenging activity (O2●-, OH-, H2O2). The ability of the extracts to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Baccharis incarum; Baccharis boliviensis; Chuquiraga atacamensis; Parastrephia lucida; Anti-inflammatory activity; Cyclooxygenase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900003

One hundred million years of interhemispheric communication: the history of the corpus callosum
Provedor de dados: 56
Autores: Aboitiz,F.; Montiel,J.
Analysis of regional corpus callosum fiber composition reveals that callosal regions connecting primary and secondary sensory areas tend to have higher proportions of coarse-diameter, highly myelinated fibers than callosal regions connecting so-called higher-order areas. This suggests that in primary/secondary sensory areas there are strong timing constraints for interhemispheric communication, which may be related to the process of midline fusion of the two sensory hemifields across the hemispheres. We postulate that the evolutionary origin of the corpus callosum in placental mammals is related to the mechanism of midline fusion in the sensory cortices, which only in mammals receive a topographically organized representation of the sensory surfaces. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Commissures; Evolution; Interhemispheric transfer; Lateralization; Synchronization.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400002

Transplantation of rat-derived microglial cells promotes functional recovery in a rat model of spinal cord injury
Provedor de dados: 56
Autores: Kou,Dewei; Li,Tianmi; Liu,Hong; Liu,Chuansheng; Yin,Yanwei; Wu,Xing; Yu,Tengbo.
This study evaluated the effect of microglia transplantation on neurological functional recovery in rats subjected to traumatic spinal cord injury (SCI). The rat model of SCI was established using a weight drop device. Forty SCI rats were randomly divided into the microglia group and the saline group. Then, rat-derived microglial cells or normal saline was injected into the injured site 7 days after surgery. The Basso-Beattie-Bresnahan (BBB) score, inclined plate test, and motor-evoked potentials (MEPs) were applied to assess the recovery of motor function. Hematoxylin and eosin (H&amp;E) staining was used to assess the therapeutic effect. Microglia transplantation significantly improved BBB scores and functional scores at 2, 3, 4, 6, and 8 weeks after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microglia; Spinal cord injury; Transplantation; CD68; OX42.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000603

Negative emotions affect postoperative scores for evaluating functional knee recovery and quality of life after total knee replacement
Provedor de dados: 56
Autores: Qi,A.; Lin,C.; Zhou,A.; Du,J.; Jia,X.; Sun,L.; Zhang,G.; Zhang,L.; Liu,M.
This study aimed to determine whether psychological factors affect health-related quality of life (HRQL) and recovery of knee function in total knee replacement (TKR) patients. A total of 119 TKR patients (male: 38; female: 81) completed the Beck Anxiety Inventory (BAI), Beck Depression Inventory (BDI), State Trait Anxiety Inventory (STAI), Eysenck Personality Questionnaire-revised (EPQR-S), Knee Society Score (KSS), and HRQL (SF-36). At 1 and 6 months after surgery, anxiety, depression, and KSS scores in TKR patients were significantly better compared with those preoperatively (P&lt;0.05). SF-36 scores at the sixth month after surgery were significantly improved compared with preoperative scores (P&lt;0.001). Preoperative Physical Component Summary Scale...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total knee replacement; Negative emotion; Anxiety; Depression; Functional recovery; Health-related quality of life.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100602

In silico analysis identifies a C3HC4-RING finger domain of a putative E3 ubiquitin-protein ligase located at the C-terminus of a polyglutamine-containing protein
Provedor de dados: 56
Autores: Scior,T.; Luna,F.; Koch,W.; Sánchez-Ruiz,J.F.
Almost identical polyglutamine-containing proteins with unknown structures have been found in human, mouse and rat genomes (GenBank AJ277365, AF525300, AY879229). We infer that an identical new gene (RING) finger domain of real interest is located in each C-terminal segment. A three-dimensional (3-D) model was generated by remote homology modeling and the functional implications are discussed. The model consists of 65 residues from terminal position 707 to 772 of the human protein with a total length of 796 residues. The 3-D model predicts a ubiquitin-protein ligase (E3) as a binding site for ubiquitin-conjugating enzyme (E2). Both enzymes are part of the ubiquitin pathway to label unwanted proteins for subsequent enzymatic degradation. The molecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E3 ubiquitin-protein ligase; RING finger structure; Low homology model.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300002

BCG lymphadenopathy detected in a BCG-vaccinated infant
Provedor de dados: 56
Autores: Barouni,A.S.; Augusto,C.; Queiroz,M.V.N.P.; Lopes,M.T.P.; Zanini,M.S.; Salas,C.E.
Large-scale vaccination with BCG, the live attenuated strain of Mycobacterium bovis, is being adopted around the world, although sporadic complications have occurred after the procedure. Lymphadenopathy is not uncommon especially in babies under one year (0.73% of vaccinated infants), but the swelling subsides within 2 months in most cases, with no medical or surgical treatment. Brazil adopted BCG vaccination program earlier in the seventies and by 1995 more than 96% of the infant population received this immunization. We report here the occurrence of lymphadenopathy in a two-year-old child vaccinated with the Brazilian BCG strain. The diagnosis was made using a lymph node biopsy and intestinal aspirates that yielded a positive mycobacterial culture. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bacille Calmette-Guérin (BCG); Vaccination; Polymerase chain reaction; Lymphadenopathy; Antibiotic resistance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500011

Lymphocyte subpopulations during cytomegalovirus disease in renal transplant recipients
Provedor de dados: 56
Autores: Castro,S.M.; Sporleder,H.; Schröeder,R.; Santos,A.; Garcia,V.; Neumann,J.; Costa,S.C.B.
We have determined the number of circulating T, B and natural killer cells in renal transplant recipients in order to detect changes during cytomegalovirus (CMV) infections. Serial blood samples were taken from 61 patients on standard triple immunosuppression therapy (cyclosporin A, azathioprine and prednisone). Using two-color flow cytometry analysis, the absolute number of CD3+, CD4+, CD8+, CD19+, CD3+HLA-DR+ and CD16+56+ cells was determined. Forty-eight patients (78.7%) developed active CMV infection, and all of them subsequently recovered. Twenty of the infected patients (32.8%) presented symptoms compatible with CMV disease during the infectious process. The number of lymphocytes and their main subpopulations were normal before the onset of CMV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transplantation; Cytomegalovirus; Antigenemia; T lymphocytes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600016

Salinomycin enhances doxorubicin sensitivity through reversing the epithelial-mesenchymal transition of cholangiocarcinoma cells by regulating ARK5
Provedor de dados: 56
Autores: Yu,Z.; Cheng,H.; Zhu,H.; Cao,M.; Lu,C.; Bao,S.; Pan,Y.; Li,Y.
Chemotherapy response rates in patients with cholangiocarcinoma remain low, primarily due to the development of drug resistance. Epithelial-mesenchymal transition (EMT) of cancer cells is widely accepted to be important for metastasis and progression, but it has also been linked to the development of chemoresistance. Salinomycin (an antibiotic) has shown some potential as a chemotherapeutic agent as it selectively kills cancer stem cells, and has been hypothesized to block the EMT process. In this study, we investigated whether salinomycin could reverse the chemoresistance of cholangiocarcinoma cells to the chemotherapy drug doxorubicin. We found that combined salinomycin with doxorubicin treatment resulted in a significant decrease in cell viability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemotherapy; Cholangiocarcinoma; Drug resistance; Doxorubicin; Salinomycin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000607

Identification of Mycobacterium bovis antigens by analysis of bovine T-cell responses after infection with a virulent strain
Provedor de dados: 56
Autores: Alito,A.; McNair,J.; Girvin,R.M.; Zumarraga,M.; Bigi,F.; Pollock,J.M.; Cataldi,A.
Purification and characterization of individual antigenic proteins are essential for the understanding of the pathogenic mechanisms of mycobacteria and the immune response against them. In the present study, we used anion-exchange chromatography to fractionate cell extracts and culture supernatant proteins from Mycobacterium bovis to identify T-cell-stimulating antigens. These fractions were incubated with peripheral blood mononuclear cells (PBMC) from M. bovis-infected cattle in lymphoproliferation assays. This procedure does not denature proteins and permits the testing of mixtures of potential antigens that could be later identified. We characterized protein fractions with high stimulation indices from both culture supernatants and cell extracts....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine tuberculosis; Antigens; Mycobacterium bovis; Cellular immunity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100011

A calcineurin inhibitory protein overexpressed in Down's syndrome interacts with the product of a ubiquitously expressed transcript
Provedor de dados: 56
Autores: Silveira,H.C.S.; Sommer,C.A.; Soares-Costa,A.; Henrique-Silva,F.
The Down's syndrome candidate region 1 (DSCR1) protein, encoded by a gene located in the human chromosome 21, interacts with calcineurin and is overexpressed in Down's syndrome patients. As an approach to clarifying a putative function for this protein, in the present study we used the yeast two-hybrid system to identify DSCR1 partners. The two-hybrid system is a method that allows the identification of protein-protein interactions through reconstitution of the activity of the yeast GAL 4 transcriptional activator. The gene DSCR1 fused to the GAL 4 binding domain (BD) was used to screen a human fetal brain cDNA library cloned in fusion with the GAL 4 activation domain (AD). Three positive clones were found and sequence analysis revealed that all the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Down's syndrome; Down's syndrome candidate region 1; Calcineurin; Two-hybrid system; Ubiquitously expressed transcript; Protein interactions.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600002

Long-term habituation (LTH) in the crab Chasmagnathus: a model for behavioral and mechanistic studies of memory
Provedor de dados: 56
A decade of studies on long-term habituation (LTH) in the crab Chasmagnathus is reviewed. Upon sudden presentation of a passing object overhead, the crab reacts with an escape response that habituates promptly and for at least five days. LTH proved to be an instance of associative memory and showed context, stimulus frequency and circadian phase specificity. A strong training protocol (STP) (&lt;FONT FACE="Symbol"&gt;³&lt;/font&gt;15 trials, intertrial interval (ITI) of 171 s) invariably yielded LTH, while a weak training protocol (WTP) (&lt;FONT FACE="Symbol"&gt;£&lt;/font&gt;10 trials, ITI = 171 s) invariably failed. STP was used with a presumably amnestic agent and WTP with a presumably hypermnestic agent. Remarkably, systemic administration of low...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Memory; Massed training; Spaced training; Cycloheximide; PKA; Angiotensin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700001

Effect of the consumption of tomato paste on plasma prostate-specific antigen levels in patients with benign prostate hyperplasia
Provedor de dados: 56
Autores: Edinger,M.S.; Koff,W.J.
The consumption of tomatoes and tomato products has been associated with a reduced risk of prostate cancer. We observed a decrease of 10.77% in prostate-specific antigen (PSA) levels in patients with benign prostate hyperplasia who were submitted to daily ingestion of tomato paste. This was an experimental rather than a controlled study with a sample of 43 men ranging in age from 45 to 75 years, all with histological diagnoses of benign prostate hyperplasia and plasma PSA levels of 4-10 ng/mL. All patients received 50 g of tomato paste once a day for 10 consecutive weeks and PSA levels were analyzed before, during and after the consumption of tomato paste. ANOVA for repeated measures was used to compare PSA levels before, during and after the consumption...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prostate cancer; Prostate-specific antigen; Tomatoes; Tomato paste; Lycopene.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800014

Mutation and genomic amplification of the PIK3CA proto-oncogene in pituitary adenomas
Provedor de dados: 56
Autores: Murat,C.B.; Braga,P.B.S.; Fortes,M.A.H.Z.; Bronstein,M.D.; Corrêa-Giannella,M.L.C.; Giorgi,R.R.
The tumorigenesis of pituitary adenomas is poorly understood. Mutations of the PIK3CA proto-oncogene, which encodes the p110-α catalytic subunit of PI3K, have been reported in various types of human cancers regarding the role of the gene in cell proliferation and survival through activation of the PI3K/Akt signaling pathway. Only one Chinese study described somatic mutations and amplification of the PIK3CA gene in a large series of pituitary adenomas. The aim of the present study was to determine genetic alterations of PIK3CA in a second series that consisted of 33 pituitary adenomas of different subtypes diagnosed by immunohistochemistry: 6 adrenocorticotropic hormone-secreting microadenomas, 5 growth hormone-secreting macroadenomas, 7 prolactin-secreting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary adenomas; PIK3CA proto-oncogene; Genomic amplification; Somatic mutation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900009

Sertraline inhibits formalin-induced nociception and cardiovascular responses
Provedor de dados: 56
Autores: Santuzzi,C.H.; Futuro Neto,H.A.; Pires,J.G.P.; Gonçalves,W.L.S.; Tiradentes,R.V.; Gouvea,S.A.; Abreu,G.R.
The objective of the present study was to determine the antihyperalgesic effect of sertraline, measured indirectly by the changes of sciatic afferent nerve activity, and its effects on cardiorespiratory parameters, using the model of formalin-induced inflammatory nociception in anesthetized rats. Serum serotonin (5-HT) levels were measured in order to test their correlation with the analgesic effect. Male Wistar rats (250-300 g) were divided into 4 groups (N = 8/per group): sertraline-treated group (Sert + Saline (Sal) and Sert + Formalin (Form); 3 mg·kg-1·day-1, ip, for 7 days) and saline-treated group (Sal + Sal and Sal + Form). The rats were injected with 5% (50 µL) formalin or saline into the right hind paw. Sciatic nerve activity was recorded using a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sertraline; Formalin-induced pain; Antinociceptive effect; Cardiovascular system; Sciatic nerve activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100008

Organic solvent exposure and contrast sensitivity: comparing men and women
Provedor de dados: 56
Autores: Oliveira,A.R.; Campos Neto,A.A.; de Andrade,M.J.O.; de Medeiros,P.C.B.; dos Santos,N.A.
The goal of this study was to compare the visual contrast sensitivity (CS) of men and women exposed and not exposed to organic solvents. Forty-six volunteers of both genders aged between 18 and 41 years (mean±SD=27.72±6.28) participated. Gas station attendants were exposed to gas containing 46.30 ppm of solvents at a temperature of 304±274.39 K, humidity of 62.25±7.59% and ventilation of 0.69±0.46 m/s (a passive gas chromatography-based sampling method was used considering the microclimate variables). Visual CS was measured via the psychophysical method of two-alternative forced choice using vertical sinusoidal gratings with spatial frequencies of 0.2, 0.5, 1.0, 2.0, 5.0, 10.0, and 16.0 cpd (cycles per degree) and an average luminance of 34.4 cd/m2. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visual perception; Spatial frequencies; Neurotoxic agents; Chronic exposure; Gender.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300603

Hepatic capillariasis in rats: a new model for testing antifibrotic drugs
Provedor de dados: 56
Autores: Souza,M.M. de; Silva,L.M.; Barbosa Jr.,A.A.; de Oliveira,I.R.; Paraná,R.; Andrade,Z.A.
Rats infected with the helminth Capillaria hepatica regularly develop septal hepatic fibrosis that may progress to cirrhosis in a relatively short time. Because of such characteristics, this experimental model was selected for testing drugs exhibiting antifibrosis potential, such as pentoxifylline, gadolinium chloride and vitamin A. Hepatic fibrosis was qualitatively and quantitatively evaluated in liver samples obtained by partial hepatectomy and at autopsy. The material was submitted to histological, biochemical and morphometric methods. A statistically significant reduction of fibrosis was obtained with pentoxifylline when administered intraperitoneally rather than intravenously. Gadolinium chloride showed moderate activity when administered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capillaria hepatica; Hepatic fibrosis; Septal fibrosis; Antifibrotic drugs.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100011

Effect of metabolic control on parathyroid hormone secretion in diabetic patients
Provedor de dados: 56
Autores: Paula,F.J.A.; Lanna,C.M.M.; Shuhama,T.; Foss,M.C.
The metabolic derangement caused by diabetes mellitus may potentially affect bone mineral metabolism. In the present study we evaluated the effect of diabetes metabolic control on parathyroid hormone (PTH) secretion during stimulation with EDTA infusion. The study was conducted on 24 individuals, 8 of them normal subjects (group N: glycated hemoglobin - HbA1C = 4.2 ± 0.2%; range = 3.5-5.0%), 8 patients with good and regular metabolic control (group G-R: HbA1C = 7.3 ± 0.4%; range = 6.0-8.5%), and 8 patients with poor metabolic control (group P: HbA1C = 12.5 ± 1.0%; range: 10.0-18.8%). Blood samples were collected at 10-min intervals throughout the study (a basal period of 30 min and a 2-h period of EDTA infusion, 30 mg/kg body weight) and used for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus complications; Mineral metabolism; Parathormone secretion.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900006

Vincristine delays gastric emptying and gastrointestinal transit of liquid in awake rats
Provedor de dados: 56
Autores: Peixoto Júnior,A.A.; Teles,B.C.V; Castro,E.F.B.; Santos,A.A.; de Oliveira,G.R.; Ribeiro,R.A.; Rola,F.H.; Gondim,F.A.A.
We evaluated the effects of vincristine on the gastrointestinal (GI) motility of awake rats and correlated them with the course of vincristine-induced peripheral neuropathy. Vincristine or saline was injected into the tail vein of male Wistar rats (180-250 g) on alternate days: 50 µg/kg (5 doses, N = 10), 100 µg/kg (2, 3, 4 and 5 doses, N = 49) or 150 µg/kg (1, 2, or 5 doses, N = 37). Weight and stool output were measured daily for each animal. One day after completing the vincristine treatment, the animals were fasted for 24 h, gavage-fed with a test meal and sacrificed 10 min later to measure gastric emptying (GE), GI transit and colon weight. Sensory peripheral neuropathy was evaluated by hot plate testing. Chronic vincristine treatments with total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Constipation; Gastrointestinal transit; Peripheral neuropathy; Rat gastric emptying; Vincristine.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600015

Ambulatory blood pressure and Doppler echocardiographic indexes of borderline hypertensive men presenting an exaggerated blood pressure response during dynamic exercise
Provedor de dados: 56
Autores: Herkenhoff,F.L.; Vasquez,E.C.; Mill,J.G.; Lima,E.G.
Borderline hypertension (BH) has been associated with an exaggerated blood pressure (BP) response during laboratory stressors. However, the incidence of target organ damage in this condition and its relation to BP hyperreactivity is an unsettled issue. Thus, we assessed the Doppler echocardiographic profile of a group of BH men (N = 36) according to office BP measurements with exaggerated BP in the cycloergometric test. A group of normotensive men (NT, N = 36) with a normal BP response during the cycloergometric test was used as control. To assess vascular function and reactivity, all subjects were submitted to the cold pressor test. Before Doppler echocardiography, the BP profile of all subjects was evaluated by 24-h ambulatory BP monitoring. All subjects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borderline hypertension; Cardiovascular reactivity; Laboratory stress test; Doppler echocardiography.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000008

Effect of antibodies to intercellular adhesion molecule type 1 on the protection of distant organs during reperfusion syndrome in rats
Provedor de dados: 56
Autores: Souza-Moraes,M.R.; David-Filho,R.; Baptista-Silva,J.C.C.; Ullian,M.; Franco,M.F.; Gabriel Jr.,A.; Smith,B.; Burihan,E.
We investigated kidney and lung alterations caused by intercellular adhesion molecule type 1 (ICAM-1) blockade after ischemia and reperfusion of hind limb skeletal muscles. Rats were submitted to ligature of the infrarenal aorta for 6 h. The animals were randomized into three groups of 6 rats each: group I, sacrificed after ischemia; group II, reperfusion for 24 h, and group III, reperfusion for 24 h after receiving monoclonal anti-ICAM-1 antibodies. At the end of the experiment, blood samples were collected for creatinine, lactate dehydrogenase, creatine phosphokinase, potassium, pH and leukocyte counts. Samples were taken from the muscles of the hind limbs and from the kidneys and lungs for histological analysis and measurement of the neutrophil...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ICAM-1; Neutrophil; Myeloperoxidase; Kidney; Lung; Reperfusion.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500007

Cardiovascular responses to microinjection of trans-(±)-ACPD into the NTS were similar in conscious and chloralose-anesthetized rats
Provedor de dados: 56
Autores: Machado,B.H.; Bonagamba,L.G.H.
The changes in mean arterial pressure (MAP) and heart rate (HR) in response to the activation of metabotropic receptors in the nucleus tractus solitarii (NTS) with trans-(±)-1-amino-1,3-cyclopentanedicarboxylic acid (trans-(±)-ACPD) were evaluated in conscious and anesthetized Wistar, male rats weighing 240-260 g (N = 8). The responses obtained with trans-(±)-ACPD were compared with the responses to L-glutamate (1 nmol/100 nl), since in a previous study we showed that anesthesia converted a pressor response to L-glutamate microinjected into the NTS of conscious rats to a depressor response in the same rats under urethane or chloralose anesthesia. Microinjection of 3 doses of trans-(±)-ACPD (100, 500 and 1000 pmol/100 nl) produced a dose-dependent fall in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trans-(±)-ACPD L-glutamate ionotropic receptors excitatory amino acid receptors neurotransmission baroreflex chloralose anesthesia; Trans-(±)-1-amino-1 3-cyclopentanedicarboxylic acid.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400016

A priori estimation of accuracy and of the number of wells to be employed in limiting dilution assays
Provedor de dados: 56
Autores: Chaui-Berlinck,J.G.; Barbuto,J.A.M.
The use of limiting dilution assay (LDA) for assessing the frequency of responders in a cell population is a method extensively used by immunologists. A series of studies addressing the statistical method of choice in an LDA have been published. However, none of these studies has addressed the point of how many wells should be employed in a given assay. The objective of this study was to demonstrate how a researcher can predict the number of wells that should be employed in order to obtain results with a given accuracy, and, therefore, to help in choosing a better experimental design to fulfill one's expectations. We present the rationale underlying the expected relative error computation based on simple binomial distributions. A series of simulated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Limiting dilution assay: accuracy; Wells; Number of; Relative error.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800010

Expression of dorsal-ventral genes during early development of Rhynchosciara americana embryos
Provedor de dados: 56
Autores: Carvalho,J.C.; Rocha,D.N.; Bruno,R.V.; Vanario-Alonso,C.E.; Abdelhay,E.
The establishment of dorsal-ventral polarity in Drosophila is a complex process which involves the action of maternal and zygotically expressed genes. Interspecific differences in the expression pattern of some of these genes have been described in other species. Here we present the expression of dorsal-ventral genes during early embryogenesis in the lower dipteran Rhynchosciara americana. The expression of four genes, the ventralizing genes snail (sna) and twist (twi) and the dorsalizing genes decapentaplegic (dpp) and zerknüllt (zen), was investigated by whole-mount in situ hybridization. Sense and antisense mRNA were transcribed in vitro using UTP-digoxigenin and hybridized at 55°C with dechorionated fixed embryos. Staining was obtained with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rhynchosciara americana; Lower dipteran; Axis patterning; Dorsal-ventral genes; Early embryogenesis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100005

Infrared coagulation versus rubber band ligation in early stage hemorrhoids
Provedor de dados: 56
Autores: Gupta,P.J.
The ideal therapy for early stages of hemorrhoids is always debated. Some are more effective but are more painful, others are less painful but their efficacy is also lower. Thus, comfort or efficacy is a major concern. In the present randomized study, a comparison is made between infrared coagulation and rubber band ligation in terms of effectiveness and discomfort. One hundred patients with second degree bleeding piles were randomized prospectively to either rubber band ligation (N = 54) or infrared coagulation (N = 46). Parameters measured included postoperative discomfort and pain, time to return to work, relief in incidence of bleeding, and recurrence rate. The mean age was 38 years (range 19-68 years). The mean duration of disease was 17.5 months...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemorrhoids; Infrared coagulation; Pain; Rubber band ligation; Piles.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000022

Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice
Provedor de dados: 56
Autores: Anziliero,D.; Weiblen,R.; Kreutz,L.C.; Spilki,F.; Flores,E.F.
The immunostimulatory properties of inactivated Parapoxvirus ovis (iPPVO) have long been investigated in different animal species and experimental settings. In this study, we investigated the effects of iPPVO on cytokine expression in mice after intraperitoneal inoculation. Spleen and sera collected from iPPVO-treated mice at intervals after inoculation were submitted to cytokine mRNA determination by real-time PCR (qPCR), serum protein concentration by ELISA, and interferon (IFN)-α/β activity by bioassay. The spleen of iPPVO-treated animals showed a significant increase in mRNA expression of all cytokines assayed, with different kinetics and magnitude. Proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α), and IL-8 mRNA peaked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; MRNA; ORF virus; Immunostimulant; Innate immune response.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200110

Antinociceptive effect of intrathecal neostigmine evaluated in rats by two different pain models
Provedor de dados: 56
Autores: Prado,W.A.; Gonçalves,A.S.
The analgesic efficacy of cholinergic agonists and anticholinesterase agents has been widely recognized. The analgesic effect obtained by activating cholinergic mechanisms, however, seems to depend on the experimental pain model utilized for its evaluation. The antinociceptive effect of intraspinal neostigmine was examined in rats submitted concurrently to the tail flick and formalin tests. Neostigmine (8.25 and 16.5 nmol) produced a dose-dependent antinociceptive effect in the tail flick test (a model of phasic pain) and reduced the first phase (phasic pain) of the animal response to formalin also in a dose-dependent manner. The second phase (tonic pain) of the response to formalin, however, was slightly reduced after a longer period of time only by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antinociception; Neostigmine; Cholinergic antinociception.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000014

Genetic analysis of the porcine group B rotavirus NSP2 gene from wild-type Brazilian strains
Provedor de dados: 56
Autores: Médici,K.C.; Barry,A.F.; Alfieri,A.F.; Alfieri,A.A.
Group B rotaviruses (RV-B) were first identified in piglet feces, being later associated with diarrhea in humans, cattle, lambs, and rats. In human beings, the virus was only described in China, India, and Bangladesh, especially infecting adults. Only a few studies concerning molecular analysis of the RV-B NSP2 gene have been conducted, and porcine RV-B has not been characterized. In the present study, three porcine wild-type RV-B strains from piglet stool samples collected from Brazilian pig herds were used for analysis. PAGE results were inconclusive for those samples, but specific amplicons of the RV-B NSP2 gene (segment 8) were obtained in a semi-nested PCR assay. The three porcine RV-B strains showed the highest nucleotide identity with the human WH1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Piglets; Diarrhea; Group B rotavirus; NSP2 gene; Phylogenetic analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100003

Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets
Provedor de dados: 56
Autores: Silvera,F.E.; Blasina,M.F.; Vaamonde,L.; Tellechea,S.; Godoy,C.; Zabala,S.; Mañana,G.; Martell,M.; Olivera,W.
Meconium aspiration syndrome causes respiratory failure after birth and in vivo monitoring of pulmonary edema is difficult. The objective of the present study was to assess hemodynamic changes and edema measured by transcardiopulmonary thermodilution in low weight newborn piglets. Additionally, the effect of early administration of sildenafil (2 mg/kg vo, 30 min after meconium aspiration) on this critical parameter was determined in the meconium aspiration syndrome model. Thirty-eight mechanically ventilated anesthetized male piglets (Sus scrofa domestica) aged 12 to 72 h (1660 ± 192 g) received diluted fresh human meconium in the airway in order to evoke pulmonary hypertension (PHT). Extravascular lung water was measured in vivo with a PiCCO monitor and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung water; Meconium aspiration syndrome; Newborn; Sildenafil; Pulmonary hypertension.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800007

Pediatric pain: prevalence, assessment, and management in a teaching hospital
Provedor de dados: 56
Autores: Linhares,M.B.M.; Doca,F.N.P.; Martinez,F.E.; Carlotti,A.P.P.; Cassiano,R.G.M.; Pfeifer,L.I.; Funayama,C.A.; Rossi,L.R.G.; Finley,G.A.
The goal of this study was to examine the prevalence, assessment and management of pediatric pain in a public teaching hospital. The study sample consisted of 121 inpatients (70 infants, 36 children, and 15 adolescents), their families, 40 physicians, and 43 nurses. All participants were interviewed except infants and children who could not communicate due to their clinical status. The interview included open-ended questions concerning the inpatients’ pain symptoms during the 24 h preceding data collection, as well as pain assessment and pharmacological/non-pharmacological management of pain. The data were obtained from 100% of the eligible inpatients. Thirty-four children/adolescents (28%) answered the questionnaire and for the other 72% (unable to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pain; Inpatients; Policy; Clinical audit.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200026

Genetic aberrations in multiple myeloma characterized by cIg-FISH: a Brazilian context
Provedor de dados: 56
Autores: Segges,P.; Braggio,E.; Minnicelli,C.; Hassan,R.; Zalcberg,I.R.; Maiolino,A.
Genetic abnormalities are critical prognostic factors for patients diagnosed with multiple myeloma (MM). This retrospective, multicenter study aimed to contribute with the genetic and clinical characterization of MM patients in a country with continental dimensions such as Brazil. Genetic abnormalities were assessed by cIg-fluorescent in situ hybridization (cIg-FISH) in a series of 152 MM patients (median age 55 years, 58.5% men). Overall, genetic abnormalities were detected in 52.7% (80/152) of patients. A 14q32 rearrangement was detected in 33.5% (n=51), including t(11;14), t(4;14) and t(14;16) in 18.4, 14.1, and 1% of cases, respectively. del(13q) was identified in 42.7% (n=65) of patients, of whom 49.2% (32/65) presented a concomitant 14q32...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiple myeloma; Cytogenetic aberrations; CIg-FISH.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500701

Reimmunization after bone marrow transplantation: current recommendations and perspectives
Provedor de dados: 56
Autores: Machado,C.M.
Autologous and allogeneic bone marrow transplantation (BMT) recipients lose immune memory of exposure to infectious agents and vaccines accumulated through a lifetime and therefore need to be revaccinated. Diphtheria toxoid, tetanus toxoid, pertussis vaccine (children &lt;7 years old), Haemophilus influenzae type b conjugate, 23-valent pneumococcal polysaccharide, inactivated influenza vaccine, inactivated polio vaccine and live-attenuated measles-mumps-rubella vaccine are the currently recommended vaccines to be included in a vaccination program after BMT. For most of them, the best time to vaccinate, the number of vaccine doses and/or the duration of immunity after vaccination have not been established. Vaccination protocols vary greatly among BMT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow transplantation; Vaccination; Autologous; Allogeneic; Toxoid; Polysaccharide.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100021

Propranolol inhibits the proliferation, migration and tube formation of hemangioma cells through HIF-1α dependent mechanisms
Provedor de dados: 56
Autores: Chen,Y.Z.; Bai,N.; Bi,J.H.; Liu,X.W.; Xu,G.Q.; Zhang,L.F.; Li,X.Q.; Huo,R.
The aim of this study was to investigate the mechanism of propranolol on the regression of hemangiomas. Propranolol-treated hemangioma tissues were collected and the expression of hypoxia inducible factor-1α (HIF-1α) was examined. We also established HIF-1α overexpression and knockdown hemangioma cells, and determined the effects of HIF-1α on the hemangioma cells proliferation, apoptosis, migration and tube formation. Significantly increased HIF-1α level was found in the hemangioma tissues compared to that in normal vascular tissues, whereas propranolol treatment decreased the HIF-1α level in hemangioma tissues in a time- and dose-dependent manner. Moreover, propranolol treatment significantly decreased cell proliferation, migration and tube formation as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propranolol; Hemangiomas; Hypoxia inducible factor-1α; Knockdown; Regression.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200601

Limb immobilization alters functional electrophysiological parameters of sciatic nerve
Provedor de dados: 56
Autores: Alves,J.S.M.; Leal-Cardoso,J.H.; Santos-Júnior,F.F.U.; Carlos,P.S.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Ceccatto,V.M.; Barbosa,R.
Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Sciatic nerve; Compound action potential; Conduction velocity; Rheobase; Chronaxy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715

Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
Provedor de dados: 56
Autores: Baptista,M.S.; Alves,M.J.M.; Arantes,G.M.; Armelin,H.A.; Augusto,O.; Baldini,R.L.; Basseres,D.S.; Bechara,E.J.H.; Bruni-Cardoso,A.; Chaimovich,H.; Colepicolo Neto,P.; Colli,W.; Cuccovia,I.M.; Da-Silva,A.M.; Di Mascio,P.; Farah,S.C.; Ferreira,C.; Forti,F.L.; Giordano,R.J.; Gomes,S.L.; Gueiros Filho,F.J.; Hoch,N.C.; Hotta,C.T.; Labriola,L.; Lameu,C.; Machini,M.T.; Malnic,B.; Marana,S.R.; Medeiros,M.H.G.; Meotti,F.C.; Miyamoto,S.; Oliveira,C.C.; Souza-Pinto,N.C.; Reis,E.M.; Ronsein,G.E.; Salinas,R.K.; Schechtman,D.; Schreier,S.; Setubal,J.C.; Sogayar,M.C.; Souza,G.M.; Terra,W.R.; Truzzi,D.R.; Ulrich,H.; Verjovski-Almeida,S.; Winck,F.V.; Zingales,B.; Kowaltowski,A.J.
The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Scientific journals; Scientific editing; Pre-prints; Open access; Peer review.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900401

Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia
Provedor de dados: 56
Autores: Eik Filho,W.; Marcon,S.S.; Krupek,T.; Previdelli,I.T.S.; Pereira,O.C.N.; Silva,M.A.R.C.P.; Bazotte,R.B.
We evaluated the impact of postprandial glycemia on blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in non-diabetic patients with symptoms suggesting reactive hypoglycemia. Eleven patients with clinical symptoms suggesting reactive hypoglycemia received an oral glucose solution (75 g) Blood was collected at 0 (baseline), 30, 60, 120 and 180 min after glucose ingestion and the plasma concentrations of interferon-α (IFN-α), interferon-γ (IFN-γ), interleukin-1 receptor antagonist (IL-1RA), interleukin 2 (IL-2), interleukin-2 receptor (IL-2R), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin-12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pro-inflammatory cytokines; Anti-inflammatory cytokines; Glucose tolerance test; Inflammation; Postprandial glycemia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800702

Association of sulfur dioxide exposure with circulatory system deaths in a medium-sized city in Brazil
Provedor de dados: 56
Autores: Amancio,C.T.; Nascimento,L.F.C.
There is a demonstrable association between exposure to air pollutants and deaths due to cardiovascular diseases. The objective of this study was to estimate the effects of exposure to sulfur dioxide on mortality due to circulatory diseases in individuals 50 years of age or older residing in São José dos Campos, SP. This was a time-series ecological study for the years 2003 to 2007 using information on deaths due to circulatory disease obtained from Datasus reports. Data on daily levels of pollutants, particulate matter, sulfur dioxide (SO2), ozone, temperature, and humidity were obtained from the São Paulo State Environmental Agency. Moving average models for 2 to 7 days were calculated by Poisson regression using the R software. Exposure to SO2 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollution; Sulfur dioxide; Cardiovascular diseases; Mortality; Air pollutants; Stroke.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100013

Diagnosis of Smith-Lemli-Opitz syndrome by ultraviolet spectrophotometry
Provedor de dados: 56
Autores: Scalco,F.B.; Cruzes,V.M.; Vendramini,R.C.; Brunetti,I.L.; Moretti-Ferreira,D.
Smith-Lemli-Opitz syndrome (SLOS) is an autosomal recessive disorder due to an inborn error of cholesterol metabolism, characterized by congenital malformations, dysmorphism of multiple organs, mental retardation and delayed neuropsychomotor development resulting from cholesterol biosynthesis deficiency. A defect in 3ß-hydroxysteroid-delta7-reductase (delta7-sterol-reductase), responsible for the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol, causes an increase in 7-DHC and frequently reduces plasma cholesterol levels. The clinical diagnosis of SLOS cannot always be conclusive because of the remarkable variability of clinical expression of the disorder. Thus, confirmation by the measurement of plasma 7-DHC levels is needed. In the present...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smith-Lemli-Opitz; 3ß-Hydroxysteroid-delta-7-reductase; Cholesterol; 7-Dehydrocholesterol; Ultraviolet spectrophotometry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000008

Changes in the fecal concentrations of cortisol and androgen metabolites in captive male jaguars (Panthera onca) in response to stress
Provedor de dados: 56
Autores: Morato,R.G.; Bueno,M.G.; Malmheister,P.; Verreschi,I.T.N.; Barnabe,R.C.
In the present study we determined the efficacy of the measurement of fecal cortisol and androgen metabolite concentrations to monitor adrenal and testicular activity in the jaguar (Panthera onca). Three captive male jaguars were chemically restrained and electroejaculated once or twice within a period of two months. Fecal samples were collected daily for 5 days before and 5 days after the procedure and stored at -20ºC until extraction. Variations in the concentrations of cortisol and androgen metabolites before and after the procedure were determined by solid phase cortisol and testosterone radioimmunoassay and feces dry weight was determined by drying at 37ºC for 24 h under vacuum. On four occasions, fecal cortisol metabolite levels were elevated above...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fecal cortisol metabolite; Fecal androgen metabolite; Jaguar (Pantera onca); Captivity; Chemical restraint; Electroejaculation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200017

Effects of herbal medicine Sijunzi decoction on rabbits after relieving intestinal obstruction
Provedor de dados: 56
Autores: Li,L.; Zou,C.; Zhou,Z.; Yu,X.
Intestinal obstruction leads to blockage of the movement of intestinal contents. After relieving the obstruction, patients might still suffer with compromised immune function and nutritional deficiency. This study aimed to evaluate the effects of Sijunzi decoction on restoring the immune function and nutritional status after relieving the obstruction. Experimental rabbits (2.5±0.2 kg) were randomly divided into normal control group, 2-day intestinal obstruction group, 2-day natural recovery group, 4-day natural recovery group, 2-day treated group, and 4-day treated group. Sijunzi decoction was given twice a day to the treated groups. The concentration of markers was analyzed to evaluate the immune function and nutritional status. The concentration of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative medicine; Herbal medicine; Immune function; Intestinal obstruction; Nutritional status.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100608

Recombinant vaccines and the development of new vaccine strategies
Provedor de dados: 56
Autores: Nascimento,I.P.; Leite,L.C.C.
Vaccines were initially developed on an empirical basis, relying mostly on attenuation or inactivation of pathogens. Advances in immunology, molecular biology, biochemistry, genomics, and proteomics have added new perspectives to the vaccinology field. The use of recombinant proteins allows the targeting of immune responses focused against few protective antigens. There are a variety of expression systems with different advantages, allowing the production of large quantities of proteins depending on the required characteristics. Live recombinant bacteria or viral vectors effectively stimulate the immune system as in natural infections and have intrinsic adjuvant properties. DNA vaccines, which consist of non-replicating plasmids, can induce strong...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human vaccines; Vectors for immunization; Safety; Recombinant vaccines.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200001

Combined effects of diethylpropion and alcohol on locomotor activity of mice: participation of the dopaminergic and opioid systems
Provedor de dados: 56
Autores: Gevaerd,M.S.; Sultowski,E.T.; Takahashi,R.N.
The widespread consumption of anorectics and combined anorectic + alcohol misuse are problems in Brazil. In order to better understand the interactive effects of ethanol (EtOH) and diethylpropion (DEP) we examined the locomotion-activating effects of these drugs given alone or in combination in mice. We also determined whether this response was affected by dopamine (DA) or opioid receptor antagonists. A total of 160 male Swiss mice weighing approximately 30 g were divided into groups of 8 animals per group. The animals were treated daily for 7 consecutive days with combined EtOH + DEP (1.2 g/kg and 5.0 mg/kg, ip), EtOH (1.2 g/kg, ip), DEP (5.0 mg/kg, ip) or the control solution coadministered with the DA antagonist haloperidol (HAL, 0.075 mg/kg, ip), the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diethylpropion; Ethanol; Locomotor activity; Dopamine; Opioid; Mice.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200015

Impact of marked weight loss induced by bariatric surgery on bone mineral density and remodeling
Provedor de dados: 56
Autores: Pereira,F.A.; Castro,J.A.S. de; Santos,J.E. dos; Foss,M.C.; Paula,F.J.A.
Data about the impact of bariatric surgery (BS) and subsequent weight loss on bone are limited. The objective of the present study was to determine bone mineral density (BMD), bone remodeling metabolites and hormones that influence bone trophism in premenopausal women submitted to BS 9.8 months, on average, before the study (OGg, N = 16). The data were compared to those obtained for women of normal weight (CG, N = 11) and for obese women (OG, N = 12). Eight patients in each group were monitored for one year, with the determination of BMD, of serum calcium, phosphorus, magnesium, parathyroid hormone, 25-hydroxyvitamin D, insulin-like growth factor-I (IGF-I) and osteocalcin, and of urinary calcium and deoxypyridinoline. The biochemical determinations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Obesity; Bone mineral density; Bone turnover; Bariatric surgery.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400009

Production of L1 protein from different types of HPV in Pichia pastoris using an integrative vector
Provedor de dados: 56
Autores: Coimbra,E.C.; Gomes,F.B.; Campos,J.F.; D’arc,M.; Carvalho,J.C.; Mariz,F.C.; Jesus,A.L.S.; Stocco,R.C.; Beçak,W.; Freitas,A.C.
Human papillomavirus (HPV) infection is the most common sexually transmitted disease in the world and is related to the etiology of cervical cancer. The most common high-risk HPV types are 16 and 18; however, the second most prevalent type in the Midwestern region of Brazil is HPV-33. New vaccine strategies against HPV have shown that virus-like particles (VLP) of the major capsid protein (L1) induce efficient production of antibodies, which confer protection against the same viral type. The methylotrophic yeast Pichia pastoris is an efficient and inexpensive expression system for the production of high levels of heterologous proteins stably using a wild-type gene in combination with an integrative vector. It was recently demonstrated that P. pastoris can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical cancer; Pichia pastoris; L1.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200003

Human prophylactic vaccine adjuvants and their determinant role in new vaccine formulations
Provedor de dados: 56
Autores: Pérez,O.; Batista-Duharte,A.; González,E.; Zayas,C.; Balboa,J.; Cuello,M.; Cabrera,O.; Lastre,M.; Schijns,V.E.J.C.
Adjuvants have been considered for a long time to be an accessory and empirical component of vaccine formulations. However, accumulating evidence of their crucial role in initiating and directing the immune response has increased our awareness of the importance of adjuvant research in the past decade. Nevertheless, the importance of adjuvants still is not fully realized by many researchers working in the vaccine field, who are involved mostly in the search for better target antigens. The choice of a proper adjuvant can be determinant for obtaining the best results for a given vaccine candidate, but it is restricted due to intellectual property and know-how issues. Consequently, in most cases the selected adjuvant continues to be the aluminum salt, which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human vaccines; Adjuvants; Finlay adjuvants; AFPL1; AFCo1.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800001

Comparative study of human exposure to mercury in riverside communities in the Amazon region
Provedor de dados: 56
Autores: Pinheiro,M.C.N.; Oikawa,T.; Vieira,J.L.F.; Gomes,M.S.V.; Guimarães,G.A.; Crespo-López,M.E.; Müller,R.C.S.; Amoras,W.W.; Ribeiro,D.R.G.; Rodrigues,A.R.; Côrtes,M.I.T.
Four populations in the Amazon area were selected for a comparative study of mercury-exposed and non-exposed populations: São Luiz do Tapajós, Barreiras, Panacauera, and Pindobal Grande. The highest mercury levels in human hair samples were found in São Luiz do Tapajós and Barreiras, greatly exceeding the limits established by the World Health Organization. Panacauera showed an intermediate level below 9 µg/g. This was the first comparative and simultaneous evaluation of mercury exposure in the Amazon area. Also, thanks to this type of monitoring, we were able to eliminate the uncertainties about the reference dose. On the basis of these data, we can conclude that the mercury levels detected in exposed populations of the Tapajós River basin may be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Amazon; Comparative study; Riverside populations; Human exposure; Reference dose.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300012

Profile of three Brazilian birth cohort studies in Ribeirão Preto, SP and São Luís, MA
Provedor de dados: 56
Autores: Cardoso,V.C.; Simões,V.M.F.; Barbieri,M.A.; Silva,A.A.M.; Bettiol,H.; Alves,M.T.S.S.B.; Goldani,M.Z.
We describe three birth cohort studies, respectively carried out in 1978/79 and 1994 in Ribeirão Preto, a city located in the most developed region of Brazil, and in 1997/98 in São Luís, a city located in a less developed region. The objective of the present report was to describe the methods used in these three studies, presenting their history, methodological design, objectives, developments, and difficulties faced along 28 years of research. The first Ribeirão Preto study, initially perinatal, later encompassed questions regarding the repercussions of intrauterine development on future growth and chronic adult diseases. The subjects were evaluated at birth (N = 6827), at school age (N = 2861), at the time of recruitment for military service (N = 2048),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cohort study; Epidemiological methods; Public health; Perinatal health; Sociodemographic conditions.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900003

In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
Provedor de dados: 56
Autores: Isa,N.M.; Abdelwahab,S.I.; Mohan,S.; Abdul,A.B.; Sukari,M.A.; Taha,M.M.E.; Syam,S.; Narrima,P.; Cheah,S.Ch.; Ahmad,S.; Mustafa,M.R.
The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boesenbergia rotunda; Boesenbergin A; Cytotoxicity; Antioxidation; Anti-inflammatory.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600008

One-year follow-up of the effects of sildenafil on pulmonary arterial hypertension and veno-occlusive disease
Provedor de dados: 56
Autores: Barreto,A.C.; Franchi,S.M.; Castro,C.R.P.; Lopes,A.A.
We hypothesized that chronic oral administration of the phosphodiesterase-5 inhibitor sildenafil could improve the exercise capacity and pulmonary hemodynamics in patients with pulmonary arterial hypertension (PAH) on the basis of previous short-term studies. We tested this hypothesis in 14 subjects with PAH, including seven patients with the idiopathic form and seven patients with atrial septal defects, but no other congenital heart abnormalities. Patients were subjected to a 6-min walk test and dyspnea was graded according to the Borg scale. Pulmonary flow and pressures were measured by Doppler echocardiography. Patients were given sildenafil, 75 mg orally three times a day, and followed up for 1 year. Sildenafil therapy resulted in the following...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sildenafil; Pulmonary hypertension; Pulmonary veno-occlusive disease; Phosphodiesterase inhibitors.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200006

Human papillomavirus infection among sexual partners attending a Sexually Transmitted Disease Clinic in Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Afonso,L.A.; Rocha,W.M.; Carestiato,F.N.; Dobao,E.A.; Pesca,L.F.; Passos,M.R.L.; Cavalcanti,S.M.B.
Cervical cancer is a major source of illness and death among women worldwide and genital infection with oncogenic human papillomavirus (HPV) its principal cause. There is evidence of the influence of the male factor in the development of cervical neoplasia. Nevertheless, the pathogenic processes of HPV in men are still poorly understood. It has been observed that different HPV types can be found among couples. The objective of the present study was to investigate HPV infections in female patients (n = 60 females/group) as well as in their sexual partners and to identify the concordance of HPV genotypes among them. By using the polymerase chain reaction, we detected a 95% prevalence of HPV DNA in women with cervical intraepithelial neoplasia (CIN) compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Neoplasia; Couples; Sexual partners; Polymerase chain reaction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600533

Cell therapy in dilated cardiomyopathy: from animal models to clinical trials
Provedor de dados: 56
Autores: del Corsso,C.; Campos de Carvalho,A.C.
Dilated cardiomyopathy can be the end-stage form and common denominator of several cardiac disorders of known cause, such as hypertensive, ischemic, diabetic and Chagasic diseases. However, some individuals have clinical findings, such as an increase in ventricular chamber size and impaired contractility (classical manifestations of dilated cardiomyopathy) even in the absence of a diagnosed primary disease. In these patients, dilated cardiomyopathy is classified as idiopathic since its etiology is obscure. Nevertheless, regardless of all of the advances in medical, pharmacological and surgical procedures, the fate of patients with dilated cardiomyopathy (of idiopathic or of any other known cause) is linked to arrhythmic episodes, severe congestive heart...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dilated cardiomyopathy; Stem cell; Heart failure; Chagas’ disease; Cell therapy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500002

Differences between remaining ability and loss of capacity in maximum aerobic impairment
Provedor de dados: 56
Autores: Neder,J.A.; Nery,L.E.; Bagatin,E.; Lucas,S.R.; Anção,M.S.; Sue,D.Y.
In the evaluation of exercise intolerance of patients with respiratory diseases the American Medical Association (AMA) and the American Thoracic Society (ATS) have proposed similar classifications for rating aerobic impairment using maximum oxygen uptake (VO2max) normalized for total body weight (ml min-1 kg-1). However, subjects with the same VO2max weight-corrected values may have considerably different losses of aerobic performance (VO2max expressed as % predicted). We have proposed a new, specific method for rating loss of aerobic capacity (VO2max, % predicted) and we have compared the two classifications in a prospective study involving 75 silicotic claimants. Logistic regression analysis showed that the disagreement between rating systems (higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disability evaluation; Work capacity; Exercise tolerance; Oxygen consumption; Occupational diseases.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500006

Proximal tubular dysfunction as an indicator of chronic graft dysfunction
Provedor de dados: 56
Autores: Câmara,N.O.S.; Williams Jr.,W.W.; Pacheco-Silva,A.
New strategies are being devised to limit the impact of renal sclerosis on graft function. Individualization of immunosuppression, specifically the interruption of calcineurin-inhibitors has been tried in order to promote better graft survival once chronic graft dysfunction has been established. However, the long-term impact of these approaches is still not totally clear. Nevertheless, patients at higher risk for tubular atrophy and interstitial fibrosis (TA/IF) development should be carefully monitored for tubular function as well as glomerular performance. Since tubular-interstitial impairment is an early event in TA/IF pathogenesis and associated with graft function, it seems reasonable that strategies directed at assessing tubular structural integrity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal transplantation; Tubular proteins; Proximal tubular dysfunction; Chronic allograft nephropathy; Retinol binding protein.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300003

Cardiorespiratory adaptations induced by aerobic training in middle-aged men: the importance of a decrease in sympathetic stimulation for the contribution of dynamic exercise tachycardia
Provedor de dados: 56
Autores: Chacon-Mikahil,M.P.T.; Forti,V.A.M.; Catai,A.M.; Szrajer,J.S.; Golfetti,R.; Martins,L.E.B.; Lima-Filho,E.C.; Wanderley,J.S.; Marin-Neto,J.A.; Maciel,B.C.; Gallo-Jr.,L.
We investigated the effects of aerobic training on the efferent autonomic control of heart rate (HR) during dynamic exercise in middle-aged men, eight of whom underwent exercise training (T) while the other seven continued their sedentary (S) life style. The training was conducted over 10 months (three 1-h sessions/week on a field track at 70-85% of the peak HR). The contribution of sympathetic and parasympathetic exercise tachycardia was determined in terms of differences in the time constant effects on the HR response obtained using a discontinuous protocol (4-min tests at 25, 50, 100 and 125 watts on a cycle ergometer), and a continuous protocol (25 watts/min until exhaustion) allowed the quantification of the parameters (anaerobic threshold, VO2 AT;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sympathetic modulation of heart rate; Dynamic exercise; Aerobic training; Autonomic nervous system; Anaerobic threshold; Middle-aged men.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500016

Modulation of ROS production in human leukocytes by ganglioside micelles
Provedor de dados: 56
Autores: Gavella,M.; Kveder,M.; Lipovac,V.
Recent studies have reported that exogenous gangliosides, the sialic acid-containing glycosphingolipids, are able to modulate many cellular functions. We examined the effect of micelles of mono- and trisialoganglioside GM1 and GT1b on the production of reactive oxygen species by stimulated human polymorphonuclear neutrophils using different spectroscopic methods. The results indicated that exogenous gangliosides did not influence extracellular superoxide anion (O2.-) generation by polymorphonuclear neutrophils activated by receptor-dependent formyl-methionyl-leucyl-phenylalanine. However, when neutrophils were stimulated by receptor-bypassing phorbol 12-myristate 13-acetate (PMA), gangliosides above their critical micellar concentrations prolonged the lag...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ganglioside micelles; Reactive oxygen species; Human polymorphonuclear neutrophils.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000004

Effect of exercise training on ventilatory efficiency in patients with heart disease: a review
Provedor de dados: 56
Autores: Prado,D.M.L.; Rocco,E.A.; Silva,A.G.; Rocco,D.F.; Pacheco,M.T.; Furlan,V.
The analysis of ventilatory efficiency in cardiopulmonary exercise testing has proven useful for assessing the presence and severity of cardiorespiratory diseases. During exercise, efficient pulmonary gas exchange is characterized by uniform matching of lung ventilation with perfusion. By contrast, mismatching is marked by inefficient pulmonary gas exchange, requiring increased ventilation for a given CO2 production. The etiology of increased and inefficient ventilatory response to exercise in heart disease is multifactorial, involving both peripheral and central mechanisms. Exercise training has been recommended as non-pharmacological treatment for patients with different chronic cardiopulmonary diseases. In this respect, previous studies have reported...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart disease; Ventilatory efficiency; Exercise test; Exercise training.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700301

Effects of chronic ethanol treatment on monoamine levels in rat hippocampus and striatum
Provedor de dados: 56
Autores: Vasconcelos,S.M.M.; Cavalcante,R.A.; Aguiar,L.M.V.; Sousa,F.C.F.; Fonteles,M.M.F.; Viana,G.S.B.
We studied the effects of ethanol on concentrations of noradrenaline (NE), dopamine (DA) and serotonin (5-HT) and their metabolites in rat hippocampus and striatum. Ethanol (2 or 4 g/kg, po, from a 20% aqueous solution) was administered daily to male Wistar rats (4-13 per group) for 30 days and animals were sacrificed 30 min or 48 h after the last administration. Monoamines were measured by HPLC and considered significant at P &lt; 0.05. A 47% increase in 5-HT levels was observed in the hippocampus with 4 g/kg ethanol in the 30-min protocol. Ethanol (2 and 4 g/kg) decreased DA (2114.5 ± 126.4 and 1785.1 ± 234.2 ng/g wet tissue, respectively) and 3,4-dihydroxyphenylacetic acid (DOPAC, 1477.6 ± 132.1 and 1218.8 ± 271.7 ng/g wet tissue, respectively) levels,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hippocampus; Striatum; Ethanol; Noradrenaline; Dopamine; Serotonin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200009

Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
Provedor de dados: 56
Autores: Lachtermacher,S.; Esporcatte,B.L.B.; Montalvão,F.; Costa,P.C.; Rodrigues,D.C.; Belem,L.; Rabischoffisky,A.; Faria Neto,H.C.C.; Vasconcellos,R.; Iacobas,S.; Iacobas,D.A.; Dohmann,H.F.R.; Spray,D.C.; Goldenberg,R.C.S.; Campos-de-Carvalho,A.C.
After myocardial infarction (MI), activation of the immune system and inflammatory mechanisms, among others, can lead to ventricular remodeling and heart failure (HF). The interaction between these systemic alterations and corresponding changes in the heart has not been extensively examined in the setting of chronic ischemia. The main purpose of this study was to investigate alterations in cardiac gene and systemic cytokine profile in mice with post-ischemic HF. Plasma was tested for IgM and IgG anti-heart reactive repertoire and inflammatory cytokines. Heart samples were assayed for gene expression by analyzing hybridization to AECOM 32k mouse microarrays. Ischemic HF significantly increased the levels of total serum IgM (by 5.2-fold) and total IgG (by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental post-ischemic heart failure; Anti-heart antibodies; Cytokines; Immunoarray; RNAm - Microarray.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400009

Measurement of serum estrogen and estrogen metabolites in pre- and postmenopausal women with osteoarthritis using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry
Provedor de dados: 56
Autores: Gao,W.L.; Wu,L.S.; Zi,J.H.; Wu,B.; Li,Y.Z.; Song,Y.C.; Cai,D.Z.
Although 17β-estradiol (E2) deficiency has been linked to the development of osteoarthritis (OA) in middle-aged women, there are few studies relating other estrogens and estrogen metabolites (EMs) to this condition. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method to measure the levels of six EMs (i.e., estrone, E2, estriol, 2-hydroxyestrone, 2-hydroxyestradiol, and 16a-hydroxyestrone) in healthy pre- and postmenopausal women and women with OA. This method had a precision ranging from 1.1 to 3.1% and a detection limit ranging from 10 to 15 pg. Compared to healthy women, serum-free E2 was lower in the luteal and postmenopausal phases in women with OA, and total serum E2 was lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogen; Estrogen metabolites; Menopause; Serum; Osteoathritis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200146

Cytotoxic activity of the dichloromethane fraction from Vernonia scorpioides (Lam.) Pers. (Asteraceae) against Ehrlich's tumor cells in mice
Provedor de dados: 56
Autores: Pagno,T.; Blind,L.Z.; Biavatti,M.W.; Kreuger,M.R.O.
Vernonia scorpioides has been widely used in Brazil to treat skin problems and chronic wounds, such as ulcers of the lower limbs and diabetic lesions. In the present study, we investigated the effect of a dichloromethane (DCM) fraction of V. scorpioides leaf extract on Ehrlich ascitic and solid tumor-bearing mice. The animals were treated once a day with the DCM fraction at a concentration of 5 mg/kg, administered ip during and after the development of the tumor. The lifespan, weight, number and type of leukocytes, number of tumor cells, volume of solid and ascitic tumors were measured. The development of the tumor with pre-treated tumor cells in vitro with the DCM fraction (5 mg/kg) was analyzed and the animals were sacrificed after 7 days. The DCM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vernonia scorpioides; Leaf extract; Antitumoral; Ehrlich ascitic tumor; Neutrophils.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100012

Polymorphisms in genes MTHFR, MTR and MTRR are not risk factors for cleft lip/palate in South Brazil
Provedor de dados: 56
Autores: Brandalize,A.P.C.; Bandinelli,E.; Borba,J.B.; Félix,T.M.; Roisenberg,I.; Schüler-Faccini,L.
Non-syndromic cleft lip and palate (CL/P) occurs due to interaction between genetic and environmental factors. Abnormalities in homocysteine metabolism may play a role in its etiology due to polymorphisms in genes involved in this pathway. Because of the involvement of MTHFR, MTR and MTRR genes with folate metabolism and the evidence that maternal use of folic acid in early pregnancy reduces the risk for CL/P, we evaluated the influence of their polymorphisms on the etiology of CL/P through a case-control study. The analyses involved 114 non-syndromic phenotypically white children with clefts (case) and 110 mothers, and 100 non-affected (control) children and their mothers. The polymorphisms 677C&gt;T of MTHFR, 2756A&gt;G of MTR, and 66A&gt;G of MTRR genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homocysteine; Cleft lip/palate; Methylenetetrahydrofolate reductase; Methionine synthase; Methionine synthase reductase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600006

A randomized double-blind clinical trial of the effect of non-absorbable oral polymyxin on infants with severe infectious diarrhea
Provedor de dados: 56
Autores: Tahan,S.; Morais,M.B.; Wehba,J.; Scaletsky,I.C.A.; Machado,A.M.O.; Silva,L.Q.C.D.; Fagundes Neto,U.
The present study evaluated the effect of non-absorbable oral polymyxin on the duodenal microflora and clinical outcome of infants with severe infectious diarrhea. Polymyxin was chosen because classic enteropathogenic Escherichia coli was more sensitive to this antibiotic. Twenty-five infants were randomly assigned to a 7-day treatment with oral polymyxin (2.5 mg/kg in 4 daily doses) or placebo. Duodenal and stool cultures were performed before and after the treatment. Five patients were excluded during the study because of introduction of parental antibiotic therapy due to clinical sepsis (N = 3) or rapid clinical improvement (N = 2). In the polymyxin group, small bowel bacterial overgrowth occurred in 61.5% of the cases (8/13) before treatment and in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infants; Infectious diarrhea; Duodenal microflora; Polymyxin; Sepsis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200007

Alternative complement pathway and factor B activities in rats with altered blood levels of thyroid hormone
Provedor de dados: 56
Autores: Bitencourt,C.S.; Duarte,C.G.; Azzolini,A.E.C.S.; Assis-Pandochi,A.I.
Evaluating the activity of the complement system under conditions of altered thyroid hormone levels might help elucidate the role of complement in triggering autoimmune processes. Here, we investigated alternative pathway (AP) activity in male Wistar rats (180 ± 10 g) after altering their thyroid hormone levels by treatment with triiodothyronine (T3), propylthiouracil (PTU) or thyroidectomy. T3 and thyroxine (T4) levels were determined by chemiluminescence assays. Hemolytic assays were performed to evaluate the lytic activity of the AP. Factor B activity was evaluated using factor B-deficient serum. An anti-human factor B antibody was used to measure factor B levels in serum by radial immunodiffusion. T3 measurements in thyroidectomized animals or animals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid hormone; Complement system; Alternative complement pathway; Factor B; T3; T4.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300006

Long-term efficacy of endovascular vs open surgical repair for complicated type-B aortic dissection: a single-center retrospective study and meta-analysis
Provedor de dados: 56
Autores: Zhu,Y.; Wang,B.; Meng,Q.; Liu,J.; Zhai,S.; He,J.
This study aimed to evaluate the long-term survival and risk factors of traditional open surgical repair (OSR) vs thoracic endovascular aneurysm repair (TEVAR) for complicated type-B aortic dissection (TBAD). A total of 118 inpatients (45 OSR vs 73 TEVAR) with TBAD were enrolled from January 2004 to January 2015. Kaplan-Meier curves and Cox proportional hazards analysis were performed to identify the long-term survival rate and independent predictors of survival, respectively. Meta-analysis was used to further explore the long-term efficacy of OSR and TEVAR in the eight included studies using Review Manager 5.2 software. An overall 10-year survival rate of 41.9% was found, and it was similar in the two groups (56.7% OSR vs 26.1% TEVAR; log-rank P=0.953)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aortic dissection; Open surgical repair; Type-B aortic dissection; Endovascular aortic repair.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600707

PDK2 promotes chondrogenic differentiation of mesenchymal stem cells by upregulation of Sox6 and activation of JNK/MAPK/ERK pathway
Provedor de dados: 56
Autores: Wang,H.; Shan,X.B.; Qiao,Y.J.
This study was undertaken to clarify the role and mechanism of pyruvate dehydrogenase kinase isoform 2 (PDK2) in chondrogenic differentiation of mesenchymal stem cells (MSCs). MSCs were isolated from femurs and tibias of Sprague-Dawley rats, weighing 300-400 g (5 females and 5 males). Overexpression and knockdown of PDK2 were transfected into MSCs and then cell viability, adhesion and migration were assessed. Additionally, the roles of aberrant PDK2 in chondrogenesis markers SRY-related high mobility group-box 6 (Sox6), type ΙΙ procollagen gene (COL2A1), cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), type ΙX procollagen gene (COL9A2) and collagen type 1 alpha 1 (COL1A1) were measured by quantitative reverse-transcription polymerase chain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyruvate dehydrogenase kinase isoform 2; Chondrogenic differentiation; Mesenchymal stem cell; SRY-related high mobility group-box 6; C-Jun N-terminal kinase (JNK).
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200606

Ghost protein damage by peroxynitrite and its protection by melatonin
Provedor de dados: 56
Autores: Di Mascio,P.; Dewez,B.; Garcia,C.R.S.
We have studied the effect of peroxynitrite (ONOO-) on the membrane cytoskeleton of red blood cells and its protection by melatonin. Analysis of the protein fraction of the preparation by SDS-PAGE revealed a dose-dependent (0-600 µM ONOO-) disappearance at pH 7.4 of the main proteins: spectrin, band 3, and actin, with the concomitant formation of high-molecular weight aggregates resistant to reduction by ß-mercaptoethanol (2%) at room temperature for 20 min. These aggregates were not solubilized by 8 M urea. Incubation of the membrane cytoskeleton with ONOO- was characterized by a marked depletion of free sulfhydryl groups (50% at 250 µM ONOO-). However, a lack of effect of ß-mercaptoethanol suggests that, under our conditions, aggregate formation is not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peroxynitrite; Melatonin; Spectrin; Protein; Ghost; Thiols.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100002

Evidence for divided automatic attention
Provedor de dados: 56
Autores: Silva,P.S.; Ribeiro-do-Valle,L.E.
A long-standing debate in the literature is whether attention can form two or more independent spatial foci in addition to the well-known unique spatial focus. There is evidence that voluntary visual attention divides in space. The possibility that this also occurs for automatic visual attention was investigated here. Thirty-six female volunteers were tested. In each trial, a prime stimulus was presented in the left or right visual hemifield. This stimulus was characterized by the blinking of a superior, middle or inferior ring, the blinking of all these rings, or the blinking of the superior and inferior rings. A target stimulus to which the volunteer should respond with the same side hand or a target stimulus to which she should not respond was presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Automatic attention; Divided attention; Visual priming; Go/no-go reaction time.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200013

Inactivation of Staphylococcus aureus and Salmonella enteritidis in tryptic soy broth and caviar samples by high pressure processing
Provedor de dados: 56
Autores: Fioretto,F.; Cruz,C.; Largeteau,A.; Sarli,T.A.; Demazeau,G.; El Moueffak,A.
We studied the action of high pressure processing on the inactivation of two foodborne pathogens, Staphylococcus aureus ATCC 6538 and Salmonella enteritidis ATCC 13076, suspended in a culture medium and inoculated into caviar samples. The baroresistance of the two pathogens in a tryptic soy broth suspension at a concentration of 10(8)-10(9) colony-forming units/ml was tested for continuous and cycled pressurization in the 150- to 550-MPa range and for 15-min treatments at room temperature. The increase of cycle number permitted the reduction of the pressure level able to totally inactivate both microorganisms in the tryptic soy broth suspension, whereas the effect of different procedure times on complete inactivation of the microorganisms inoculated into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High pressure processing; Staphylococcus aureus; Salmonella enteritidis; Tryptic soy broth; Caviar; Pressure cycles.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800015

Mechanistic effect of the human GJB6 gene and its mutations in HaCaT cell proliferation and apoptosis
Provedor de dados: 56
Autores: Lu,Yuting; Zhang,Ruili; Wang,Zhenying; Zhou,Shuhua; Song,Yali; Chen,Lamei; Chen,Nan; Liu,Wenmin; Ji,Canan; Wu,Wangli; Zhang,Li.
We constructed lentiviral vectors containing the human wild-type GJB6 gene and the mutant variants A88V and G11R. The three proteins were stably expressed by the Tet-on system in the HaCaT cell line and used to study the functional effect of the variants. The CCK-8 assay and flow cytometric analyses were used to determine the levels of cell proliferation and apoptosis. Western blot analyses were performed to analyze the relevant clinical indicators of hidrotic ectodermal dysplasia and markers of apoptosis in transfected HaCaT cells. The CCK8 assay and the flow cytometry results showed a significant increase (P&lt;0.05) in the apoptosis of HaCaT cells expressing the A88V and G11R mutants. In addition, we demonstrated that the A88V and G11R mutants induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clouston syndrome; GJB6 gene; Connexin 30; Caspase; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900611

Effects of pneumonectomy on nitric oxide synthase expression and perivascular edema in the remaining lung of rats
Provedor de dados: 56
Autores: Samano,M.N.; Pazetti,R.; Prado,C.M.; Tibério,I.C.; Saldiva,P.H.N.; Moreira,L.F.P.; Pêgo-Fernandes,P.M.; Jatene,F.B.; Das-Neves-Pereira,J.C.
Pneumonectomy is associated with high mortality and high rates of complications. Postpneumonectomy pulmonary edema is one of the leading causes of mortality. Little is known about its etiologic factors and its association with the inflammatory process. The purpose of the present study was to evaluate the role of pneumonectomy as a cause of pulmonary edema and its association with gas exchange, inflammation, nitric oxide synthase (NOS) expression and vasoconstriction. Forty-two non-specific pathogen-free Wistar rats were included in the study. Eleven animals died during or after the procedure, 21 were submitted to left pneumonectomy and 10 to sham operation. These animals were sacrificed after 48 or 72 h. Perivascular pulmonary edema was more intense in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pneumonectomy; Pulmonary edema; Nitric oxide synthase; Animal models; NOS.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100019

Pim-1 kinase inhibits the activation of reporter gene expression in Elk-1 and c-Fos reporting systems but not the endogenous gene expression: an artifact of the reporter gene assay by transient co-transfection
Provedor de dados: 56
Autores: Yan,B.; Wang,H.; Kon,T.; Li,C.-Y.
We have studied the molecular mechanism and signal transduction of pim-1, an oncogene encoding a serine-threonine kinase. This is a true oncogene which prolongs survival and inhibits apoptosis of hematopoietic cells. In order to determine whether the effects of Pim-1 occur by regulation of the mitogen-activated protein kinase pathway, we used a transcriptional reporter assay by transient co-transfection as a screening method. In this study, we found that Pim-1 inhibited the Elk-1 and NFkappaB transcriptional activities induced by activation of the mitogen-activated protein kinase cascade in reporter gene assays. However, Western blots showed that the induction of Elk-1-regulated expression of endogenous c-Fos was not affected by Pim-1. The phosphorylation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pim-1 kinase; Signal transduction; Mitogen-activated protein kinase pathway; Elk-1; Reporter assay.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200002

Expression of inducible nitric oxide synthase is increased in patients with heart failure due to ischemic disease
Provedor de dados: 56
Autores: Ferreiro,C.R.; Chagas,A.C.P.; Carvalho,M.H.C.; Dantas,A.P.; Scavone,C.; Souza,L.C.B.; Buffolo,E.; Luz,P.L. da.
The objective of the present study was to determine the relationship between nitric oxide synthases (NOS) and heart failure in cardiac tissue from patients with and without cardiac decompensation. Right atrial tissue was excised from patients with coronary artery disease (CAD) and left ventricular ejection fraction (LVEF) &lt;35% (N = 10), and from patients with CAD and LVEF &gt;60% (N = 10) during cardiac surgery. NOS activity was measured by the conversion of L-[H³]-arginine to L-[H³]-citrulline. Gene expression was quantified by the competitive reverse transcription-polymerase chain reaction. Both endothelial NOS (eNOS) activity and expression were significantly reduced in failing hearts compared to non-failing hearts: 0.36 ± 0.18 vs 1.51 ± 0.31 pmol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Left ventricle ejection fraction; Heart failure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900005

Effect of hormone replacement on exercise cardiopulmonary reserve and recovery performance in subclinical hypothyroidism
Provedor de dados: 56
Autores: Teixeira,P.F.S.; Oliveira,F.P.; Vaisman,M.
Subclinical hypothyroidism (SH) patients present cardiopulmonary, vascular and muscle dysfunction, but there is no consensus about the benefits of levothyroxine (L-T4) intervention on cardiopulmonary performance during exercise. The aim of the present study was to investigate the effects of L-T4 on cardiopulmonary exercise reserve and recovery in SH patients. Twenty-three SH women, 44 (40-50) years old, were submitted to two ergospirometry tests, with an interval of 6 months of normalization of thyroid-stimulating hormone (TSH) levels (L-T4 replacement group) or simple observation (TSH = 6.90 μIU/mL; L-T4 = 1.02 ng/dL). Patients with TSH &amp;gt;10 μIU/mL were excluded from the study to assure that they would receive treatment in this later stage of SH....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subclinical hypothyroidism Thyroid dysfunction Levothyroxine; Ergospirometry Exercise physiology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100012

The attentional modulation of the flash-lag effect
Provedor de dados: 56
Autores: Baldo,M.V.C.; Namba,J.
If a dot is flashed in perfect alignment with a pair of dots rotating around the visual fixation point, most observers perceive the rotating dots as being ahead of the flashing dot (flash-lag effect). This perceptual effect has been interpreted to result from the perceptual extrapolation of the moving dots, the differential visual latencies between flashing and moving stimuli, as well as the modulation of attentional mechanisms. Here we attempted to uncouple the attentional effects brought about by the spatial predictability of the flashing dot from the sensory effects dependent on its visual eccentricity. The stimulus was a pair of dots rotating clockwise around the fixation point. Another dot was flashed at either the upper right or the lower left of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Attention; Flash-lag effect; Vision; Psychophysics.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800014

Clinical and prognostic significance of serum transforming growth factor-beta1 levels in patients with pancreatic ductal adenocarcinoma
Provedor de dados: 56
Autores: Zhao,J.; Liang,Y.; Yin,Q.; Liu,S.; Wang,Q.; Tang,Y.; Cao,C.
Pancreatic ductal adenocarcinoma (PDAC) has a poor 5-year survival rate of 5%. Biomarkers for the early detection of pancreatic cancer are urgently needed. Transforming growth factor-beta1 (TGF-β1) is elevated in the tissues and plasma of patients with PDAC. However, no studies systemically report prognostic significance of plasma TGF-β1 levels in PDAC. In the present study, we assessed the prognostic significance of serum TGF-β levels in patients with PDAC. TGF-β levels were determined in serum from 146 PDAC patients, and 58 patients with benign pancreatic conditions. Regression models were used to correlate TGF-β levels to gender, age, stage, class, and metastasis. Survival analyses were performed using multivariate Cox models. Serum levels of TGF-β1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic ductal adenocarcinoma; Marker; TGF-β1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800705

Synthesis and evaluation of coumarin derivatives against human lung cancer cell lines
Provedor de dados: 56
Autores: Weng,K.-G.; Yuan,Y.-L.
Series of novel coumarin derivatives [I (a–d) and II (a–d)] were successfully synthesized and their structures were determined based on infrared 1H-nuclear magnetic resonance (NMR), HRMS, and single crystal X-ray crystallography. Additionally, the new synthesized compounds were evaluated to identify the molecular characteristics that contribute to their cytotoxicity, which was tested against SK-LU-1, SPC-A-1 and 95D human lung cancer cell lines, using the MTT assay. The results of this study showed that compounds Ic, Id, IIc, and IId exhibited an efficient percentage of inhibition of cell proliferation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coumarin; X-ray; Lung cancer.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100603

Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli
Provedor de dados: 56
Autores: Cui,Y.; Zhou,P.; Peng,J.; Peng,M.; Zhou,Y.; Lin,Y.; Liu,L.
Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, the cDNA fragment was cloned into the pMD19-T vector. The fragment was then sequenced, subcloned into the plasmid pET28a(+), expressed in Escherichia coli BL21 and identified by Western blotting. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Sequence analysis showed the presence of an open reading frame containing 966 bp that encodes a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatophagoides farinae; Der f 1; Recombinant allergens; Cloning; Expression; Bioinformatics.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500006

Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells
Provedor de dados: 56
Autores: Rolim,P.M.; Fidelis,G.P.; Padilha,C.E.A.; Santos,E.S.; Rocha,H.A.O.; Macedo,G.R.
Melon (Cucumis melo L.) has high economic value and in recent years, its production has increased; however, part of the fruit is wasted. Usually, inedible parts such as peel and seeds are discarded during processing and consumption. Extracts of melon residues were prepared and their phenolic compounds, antioxidants and antiproliferative activities were evaluated. Total phenolic compounds were found in hydroethanolic, hydromethanolic, and aqueous extracts, especially for melon peel (1.016 mg gallic acid equivalent/100 g). Flavonoids total content found for melon peel aqueous extract was 262 µg of catechin equivalent (CA)/100 g. In all extracts of melon peel significant amounts of gallic acid, catechin, and eugenol were found. For total antioxidant capacity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melon residues; Antioxidant activity; Cancer cells; Free radical scavenging activity; Metal chelator; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400607

LXW7 ameliorates focal cerebral ischemia injury and attenuates inflammatory responses in activated microglia in rats
Provedor de dados: 56
Autores: Fang,T.; Zhou,D.; Lu,L.; Tong,X.; Wu,J.; Yi,L.
Inflammation plays a pivotal role in ischemic stroke, when activated microglia release excessive pro-inflammatory mediators. The inhibition of integrin αvβ3 improves outcomes in rat focal cerebral ischemia models. However, the mechanisms by which microglia are neuroprotective remain unclear. This study evaluated whether post-ischemic treatment with another integrin αvβ3 inhibitor, the cyclic arginine-glycine-aspartic acid (RGD) peptide-cGRGDdvc (LXW7), alleviates cerebral ischemic injury. The anti-inflammatory effect of LXW7 in activated microglia within rat focal cerebral ischemia models was examined. A total of 108 Sprague-Dawley rats (250–280 g) were subjected to middle cerebral artery occlusion (MCAO). After 2 h, the rats were given an intravenous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Activated microglia; Ischemic stroke; Integrin αvβ3; TNF-α.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900602

Importance of SERCA2a on early isolated diastolic dysfunction induced by supravalvular aortic stenosis in rats
Provedor de dados: 56
Autores: Silveira,C.F.S.M.P.; Campos,D.H.S.; Freire,P.P.; Deus,A.F.; Okoshi,K.; Padovani,C.R.; Cicogna,A.C.
Cardiac remodeling is defined as changes in shape and function of the heart in response to aggression (pressure overload). The sarcoplasmic reticulum calcium ATPase cardiac isoform 2a (SERCA2a) is a known factor that influences function. A wide spectrum of studies report a decrease in SERCA2a in heart failure, but none evaluate it's the role in early isolated diastolic dysfunction in supravalvular aortic stenosis (AoS). Our hypothesis was that SERCA2a participates in such dysfunction. Thirty-day-old male Wistar rats (60-80 g) were divided into AoS and Sham groups, which were submitted to surgery with or without aorta clipping, respectively. After 6 weeks, the animals were submitted to echocardiogram and functional analysis by isolated papillary muscle...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Papillary muscle; Echocardiogram; Cyclopiazonic acid; Rat; Isolated diastolic dysfunction; SERCA.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500605

A clinical gamma camera-based pinhole collimated system for high resolution small animal SPECT imaging
Provedor de dados: 56
Autores: Mejia,J.; Galvis-Alonso,O.Y.; Castro,A.A.de; Braga,J.; Leite,J.P.; Simões,M.V.
The main objective of the present study was to upgrade a clinical gamma camera to obtain high resolution tomographic images of small animal organs. The system is based on a clinical gamma camera to which we have adapted a special-purpose pinhole collimator and a device for positioning and rotating the target based on a computer-controlled step motor. We developed a software tool to reconstruct the target’s three-dimensional distribution of emission from a set of planar projections, based on the maximum likelihood algorithm. We present details on the hardware and software implementation. We imaged phantoms and heart and kidneys of rats. When using pinhole collimators, the spatial resolution and sensitivity of the imaging system depend on parameters such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pinhole collimator; SPECT; Small animal organs; Maximum likelihood; Instrumentation; Image processing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200004

Effect of postnatal malnutrition on hyperoxia-induced newborn lung development
Provedor de dados: 56
Autores: Mataloun,M.M.G.B.; Leone,C.R.; Mascaretti,R.S.; Dohlnikoff,M.; Rebello,C.M.
Several factors are associated with bronchopulmonary dysplasia. Among them, hyperoxia and lung immaturity are considered to be fundamental; however, the effect of malnutrition is unknown. Our objective was to evaluate the effects of 7 days of postnatal malnutrition and hyperoxia on lung weight, volume, water content, and pulmonary morphometry of premature rabbits. After c-section, 28-day-old New Zealand white rabbits were randomized into four groups: control diet and room air (CA, N = 17), control diet and ≥95% O2 (CH, N = 17), malnutrition and room air (MA, N = 18), and malnutrition and ≥95% O2 (MH, N = 18). Malnutrition was defined as a 30% reduction of all the nutrients provided in the control diet. Treatments were maintained for 7 days, after which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Postnatal malnutrition; Hyperoxia; Lung development; Rabbit.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700004

Fertility of male adult rats submitted to forced swimming stress
Provedor de dados: 56
Autores: Mingoti,G.Z.; Pereira,R.N.; Monteiro,C.M.R.
We investigated whether stress interferes with fertility during adulthood. Male Wistar rats (weighing 220 g in the beginning of the experiment) were forced to swim for 3 min in water at 32ºC daily for 15 days. Stress was assessed by the hot-plate test after the last stressing session. To assess fertility, control and stressed males (N = 15 per group) were mated with sexually mature normal females. Males were sacrificed after copulation. Stress caused by forced swimming was demonstrated by a significant increase in the latency of the pain response in the hot-plate test (14.6 ± 1.25 s for control males vs 26.0 ± 1.53 s for stressed males, P = 0.0004). No changes were observed in body weight, testicular weight, seminal vesicle weight, ventral prostate weight...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Forced swimming stress; Male; Fertility; Rat; Spermatogonia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500016

Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
Provedor de dados: 56
Autores: Veiga-Castelli,L.C.; Rosa e Silva,J.C.; Meola,J.; Ferriani,R.A.; Yoshimoto,M.; Santos,S.A.; Squire,J.A.; Martelli,L.
Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endometrioma; Endometriosis; Comparative genomic hybridization; Chromosomal imbalances.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800014

MicroRNA-122 regulates caspase-8 and promotes the apoptosis of mouse cardiomyocytes
Provedor de dados: 56
Autores: Zhang,Z.W.; Li,H.; Chen,S.S.; Li,Y.; Cui,Z.Y.; Ma,J.
Cardiomyocyte apoptosis plays key roles in the pathogenesis of heart diseases such as myocardial infarction. MicroRNAs are important regulators of gene expression, which are also involved in the regulation of cardiomyocyte apoptosis. However, cardiomyocyte apoptosis regulated by microRNA (miR)-122 is largely unexplored. The aim of this study focused on the role of miR-122 in cardiomyocyte apoptosis. Cardiomyocytes were isolated from neonatal mice and primarily cultured. MiR-122 mimic and inhibitor were transfected to cardiomyocytes and verified by qRT-PCR. Cell viability and apoptosis post-transfection were assessed by MTT assay and flow cytometry, respectively. Changes in expression of caspase-8 were quantified by qRT-PCR and western blot. Results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiomyocyte; Apoptosis; MiR-122; Caspase-8; Myocardial infarction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200601

Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain
Provedor de dados: 56
Autores: Guillobel,H.C.R.; Luna,M.G.; Camacho,E.F.; Almeida,D.F.; Ferreira,L.C.S.
An expression plasmid (pCFA-1) carrying the cfaB gene that codes for the enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin colonization factor antigen I (CFA/I) subunit was constructed and used to transform a derivative of the attenuated Salmonella typhimurium aroA vaccine strain SL3261 carrying an F'lacIq. Treatment of the transformed strain with isopropyl-ß-D-thiogalactopyranoside (IPTG) resulted in elevated in vitro expression of the CFA/I subunit. Although flagellar function and lipopolysaccharide (LPS) synthesis were similar in both the parental and the recombinant strains, spleen colonization was reduced in the recombinant strain. All BALB/c mice parenterally inoculated with the recombinant strain developed significant anti-CFA/I and anti-LPS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella typhimurium aroA; Enterotoxigenic Escherichia coli; Attenuated vaccines; Colonization factor antigen I.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400012

Effect of gamma irradiation on the behavioral properties of crotoxin
Provedor de dados: 56
Crotoxin has been detoxified with gamma radiation in order to improve crotalic antiserum production. Nevertheless, present knowledge of the biological characteristics of irradiated crotoxin is insufficient to propose it as an immunizing agent. Crotoxin is known to increase the emotional state of rats and to decrease their exploratory behavior (Moreira EG, Nascimento N, Rosa GJM, Rogero JR and Vassilieff VS (1996) Brazilian Journal of Medical and Biological Research, 29: 629-632). Therefore, we decided 1) to evaluate the effects of crotoxin in the social interaction test, which has been widely used for the evaluation of anxiogenic drugs, and 2) to determine if irradiated crotoxin induces behavioral alterations similar to those of crotoxin in the social...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Crotoxin; Gamma-irradiated crotoxin; Anxiety; Social interaction; Hole-board test; Open-field test.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200014

Prevalence of diarrheogenic Escherichia coli and rotavirus among children from Botucatu, São Paulo State, Brazil
Provedor de dados: 56
Autores: Rodrigues,J.; Acosta,V.C.; Candeias,J.M.G.; Souza,L.O.; Filho,F.J.C.
In a one-year prospective study carried out to define the role of rotavirus and Escherichia coli in local childhood diarrhea, we determined the prevalence of both agents in 54 diarrheic children attending a health center in Botucatu. Diarrheogenic E. coli (DEC) strains were characterized by O:H serotyping, a search for virulence genetic markers, and assays of adherence to HEp-2 cells. Except for enteroaggregative E. coli (EAEC), no other DEC category was detected in the children's stools. Both EAEC and rotavirus were isolated from 22 of the 54 (41.0%) diarrheic children as single agents or in combination with other enteropathogens. However, when considering the presence of a single agent, EAEC was dominant and isolated from 20.4% of the patients, whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Rotavirus; Enteroaggregative E. coli; Escherichia coli; Seasonality.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100008

Enucleated L929 mouse fibroblasts support invasion and multiplication of Shigella flexneri 5a
Provedor de dados: 56
Autores: Yamamoto,D.; Coimbra,V.C.; Okuda,K.; Rabinovitch,M.
Invasive bacteria can induce their own uptake and specify their intracellular localization; hence it is commonly assumed that proximate modulation of host cell transcription is not required for infection. However, bacteria can also modulate, directly or indirectly, the transcription of many host cell genes, whose role in the infection may be difficult to determine by global gene expression. Is the host cell nucleus proximately required for intracellular infection and, if so, for which pathogens and at what stages of infection? Enucleated cells were previously infected with Toxoplasma gondii, Chlamydia psittaci, C. trachomatis, or Rickettsia prowazekii. We enucleated L929 mouse fibroblasts by centrifugation in the presence of cytochalasin B, and compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shigella flexneri; Cell enucleation; Cytoplasts; L929 fibroblasts.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600007

Bone mineral density in juvenile systemic lupus erythematosus
Provedor de dados: 56
Autores: Castro,T.C.M.; Terreri,M.T.; Szejnfeld,V.L.; Castro,C.H.M.; Fisberg,M.; Gabay,M.; Hilário,M.O.E.
We evaluated spine bone mineral density (BMD) in Brazilian children with juvenile systemic lupus erythematosus (JSLE) in order to detect potential predictors of reduction in bone mass. A cross-sectional study of BMD at the lumbar spine level (L2-L4) was conducted on 16 female JSLE patients aged 6-17 years. Thirty-two age-matched healthy girls were used as control. BMD at the lumbar spine was measured by dual-energy X-ray absorptiometry. Weight, height and pubertal Tanner stage were determined in patients and controls. Disease duration, mean daily steroid doses, mean cumulative steroid doses and JSLE activity measured by the systemic lupus erythematosus disease activity index (SLEDAI) were determined for all JSLE patients based on their medical charts. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Children; Adolescents; Bone mineral density; Osteoporosis; Corticosteroids.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000008

Quantification of fecal estradiol and progesterone metabolites in Syrian hamsters (Mesocricetus auratus)
Provedor de dados: 56
Autores: Chelini,M.O.M.; Souza,N.L.; Rocha,A.M.; Felippe,E.C.G.; Oliveira,C.A.
Alternative methods to the utilization of laboratory animal blood and its by-products are particularly attractive, especially regarding hamsters due to their small size and difficulties in obtaining serial blood samples. Steroid hormone metabolite quantification in feces, widely used in studies of free-ranging or intractable animals, is a non-invasive, non-stressor, economical, and animal saving technique which allows longitudinal studies by permitting frequent sampling of the same individual. The present study was undertaken to determine the suitability of this method for laboratory animals. Estradiol and progesterone metabolites were quantified by radioimmunoassay in feces of intact, sexually mature female Syrian hamsters during the estrous cycle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocrinology; Fecal steroids; Syrian hamster; Reproduction; Radioimmunoassay; Estrogen.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100021

Cardiac and vascular effects of diltiazem, dobutamine and amrinone, drugs used after myocardial revascularization
Provedor de dados: 56
Autores: Gómez-Alvis,A.; Rebolledo,A.; Milesi,V.; Raingo,J.; Sanz,N.; Tommasi,J.; Drago,A.; Rinaldi,G.; Grassi,A.
Hemodynamic care during postoperative management of myocardial revascularization should include vasorelaxing drugs to insure adequate graft and coronary flow, and stimulation of stroke volume to maintain vascular perfusion pressure. We tested the cardiac (inotropic and lusitropic) and vascular (relaxant) effects of diltiazem (0.1 nM to 0.1 mM), dobutamine (10 µM to 10 mM) and amrinone (10 µM to 1 mM) on isolated rat atria and thoracic aorta, and also on isolated human saphenous vein (HSV) and human mammary artery (HMA). Dobutamine produced a maximal positive inotropic effect (+dF/dt max = 29 ± 7%) at its ED50 for aortic relaxation (88 ± 7 µM). Conversely, at their ED50 for aortic relaxation diltiazem depressed myocardial contractility and amrinone did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amrinone; Dobutamine; Diltiazem; Saphenous vein; Mammary artery.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600015

Correction Braz J Med Biol Res 2008; 41 (7): 563-570 - 13CO2 recovery fraction in expired air of septic patients under mechanical ventilation
Provedor de dados: 56
Autores: Auxiliadora-Martins,M.; Coletto,F.A.; Martins-Filho,O.A.; Marchini,J.S.; Basile-Filho,A.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900016

Association between syndecan-1 and renal function in adolescents with excess weight: evidence of subclinical kidney disease and endothelial dysfunction
Provedor de dados: 56
Autores: Saboia,Z.M.R.M.; Meneses,G.C.; Martins,A.M.C.; Daher,E.F.; Silva Junior,G.B.
Excess weight (overweight and obesity) is associated with kidney and cardiovascular disease. The aim of this study was to investigate the association between syndecan-1 and renal function among adolescents with excess weight. A total of 56 students from a public school at Fortaleza, CE, Brazil, were investigated. The adolescents were submitted to anthropometric evaluation, including weight, height, blood pressure and body mass index. Blood and urine samples were collected for the determination of serum lipids (total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, triglycerides), and the endothelial injury biomarker syndecan-1. Participants' mean age was 16±1 years (range 14-19 years), and 68% were females. Overweight...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Overweight; Biomarkers; Kidney disease; Endothelium; Adolescents.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300606

Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells
Provedor de dados: 56
Autores: Vucic,V.; Isenovic,E.R.; Adzic,M.; Ruzdijic,S.; Radojcic,M.B.
Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma-irradiation; Prostate cancer; Proliferation; Cell death; Cell cycle arrest; Superoxide dismutase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200009

Role of the cardiac nerve in the effect of a novel innocuous stimulus on the heart rate of Megalobulimus mogianensis
Provedor de dados: 56
Autores: Romero,S.M.B.; Hoffmann,A.
The effects of a brief jet of water delivered to the anterior portion of body-head on the heart rate of Megalobulimus mogianensis were determined in a group of intact snails (N = 8), previously prepared for electrocardiogram recording. The heart rate was significantly increased following stimulation. Nevertheless, with repetition of the stimulus there was a significant decrease in the magnitude of the heart rate variation and in the time for the basal heart rate to recover (first stimulus, 7.4 ± 1.2 bpm and 15.5 ± 1.8 min; second stimulus, 4.8 ± 1.0 bpm and 10.6 ± 1.5 min; third stimulus, 5.0 ± 0.3 bpm and 11.1 ± 1.8 min), indicating that this behavioral response undergoes early habituation. To determine the role of the cardiac nerve in mediating the heart...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Innocuous stimulus; Cardiac nerve; Heart rate; Snail; Megalobulimus mogianensis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600018

Effect of protein malnutrition on the glycolytic and glutaminolytic enzyme activity of rat thymus and mesenteric lymph nodes
Provedor de dados: 56
The activity of important glycolytic enzymes (hexokinase, phosphofructokinase, aldolase, phosphohexoseisomerase, pyruvate kinase and lactate dehydrogenase) and glutaminolytic enzymes (phosphate-dependent glutaminase) was determined in the thymus and mesenteric lymph nodes of Wistar rats submitted to protein malnutrition (6% protein in the diet rather than 20%) from conception to 12 weeks after birth. The wet weight (g) of the thymus and mesenteric lymph nodes decreased due to protein malnutrition by 87% (from 0.30 ± 0.05 to 0.04 ± 0.01) and 75% (0.40 ± 0.04 to 0.10 ± 0.02), respectively. The protein content was reduced only in the thymus from 102.3 ± 4.4 (control rats) to 72.6 ± 6.6 (malnourished rats). The glycolytic enzymes were not affected by protein...
Palavras-chave: Protein malnutrition; Glucose metabolism; Glutamine metabolism; Thymus; Lymph nodes.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600003

Highly sensitive C-reactive protein and male gender are independently related to the severity of coronary disease in patients with metabolic syndrome and an acute coronary event
Provedor de dados: 56
Autores: Monteiro,C.M.C.; Pinheiro,L.F.; Izar,M.C.; Barros,S.W.; Vasco,M.B.; Fischer,S.M.; Povoa,R.M.; Brandão,S.A.; Santos,A.O.; Oliveira,L.; Carvalho,A.C.; Fonseca,F.A.H.
Patients with metabolic syndrome are at high-risk for development of atherosclerosis and cardiovascular events. The objective of this study was to examine the major determinants of coronary disease severity, including those coronary risk factors associated with metabolic syndrome, during the early period after an acute coronary episode. We tested the hypothesis that inflammatory markers, especially highly sensitive C-reactive protein (hsCRP), are related to coronary atherosclerosis, in addition to traditional coronary risk factors. Subjects of both genders aged 30 to 75 years (N = 116) were prospectively included if they had suffered a recent acute coronary syndrome (acute myocardial infarction or unstable angina pectoris requiring hospitalization) and if...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Acute coronary syndrome; C-reactive protein; Gender; Gensini score.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300012

Pharmacological differences between memory consolidation of habituation to an open field and inhibitory avoidance learning
Provedor de dados: 56
Autores: Vianna,M.R.M.; Izquierdo,L.A.; Barros,D.M.; de Souza,M.M.; Rodrigues,C.; Sant'Anna,M.K.; Medina,J.H.; Izquierdo,I.
Rats implanted bilaterally with cannulae in the CA1 region of the dorsal hippocampus or the entorhinal cortex were submitted to either a one-trial inhibitory avoidance task, or to 5 min of habituation to an open field. Immediately after training, they received intrahippocampal or intraentorhinal 0.5-µl infusions of saline, of a vehicle (2% dimethylsulfoxide in saline), of the glutamatergic N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-5-phosphono pentanoic acid (AP5), of the protein kinase A inhibitor Rp-cAMPs (0.5 µg/side), of the calcium-calmodulin protein kinase II inhibitor KN-62, of the dopaminergic D1 antagonist SCH23390, or of the mitogen-activated protein kinase kinase inhibitor PD098059. Animals were tested in each task 24 h after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Habituation learning; One-trial avoidance; Memory consolidation; Pharmacology of memory consolidation; Hippocampus; Entorhinal cortex.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200011

Strain-dependent effects of diazepam and the 5-HT2B/2C receptor antagonist SB 206553 in spontaneously hypertensive and Lewis rats tested in the elevated plus-maze
Provedor de dados: 56
Autores: Takahashi,R.N.; Berton,O.; Mormède,P.; Chaouloff,F.
The 5-HT2B/2C receptor antagonist SB 206553 exerts anxiolytic effects in rat models of anxiety. However, these effects have been reported for standard rat strains, thus raising the issue of SB 206553 effects in rat strains displaying different levels of anxiety. Herein, the effects of SB 206553 in a 5-min elevated plus-maze test of anxiety were compared to those of the reference anxiolytic, diazepam, in two rat strains respectively displaying high (Lewis rats) and low (spontaneously hypertensive rats, SHR) anxiety. Diazepam (0.37, 0.75, or 1.5 mg/kg; 30 min before testing) increased in a dose-dependent manner the behavioral measures in SHR, but not in Lewis rats. On the other hand, SB 206553 (1.25, 2.5, or 5 mg/kg; 30 min before testing) failed to alter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety; Diazepam; Elevated plus-maze; Lewis rats; Spontaneously hypertensive rats; Locomotor activity; M-chlorophenylpiperazine; SB 206553.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500017

Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats
Provedor de dados: 56
Autores: Souza,M.S.; Machado,U.F.; Okamoto,M.; Bertoluci,M.C.; Ponpermeyer,C.; Leguisamo,N.; Azambuja,F.; Irigoyen,M.C.; Schaan,B.D.
Diabetes in spontaneously hypertensive rats is associated with cortical renal GLUT1 and GLUT2 overexpression. Our objective was to evaluate the effect of the angiotensin-converting enzyme blockade on cortical renal GLUT1 and GLUT2 expression, urinary albumin and urinary TGF-β1. Streptozotocin, 50 mg/kg, or citrate buffer (N = 16) was administered as a single injection into the tail vein in adult spontaneously hypertensive rats (~260 g). Thirty days later, these diabetic spontaneously hypertensive rats received ramipril by gavage: 0.01 mg·kg-1·day-1 (D0.01, N = 14), 1 mg·kg-1·day-1 (D1, N = 9) or water (D, N = 11) for 15 days. Albumin and TGF-β1 (24-h urine), direct arterial pressure, renal tissue angiotensin-converting enzyme activity (fluorometric assay),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin-converting enzyme inhibition; Diabetes mellitus; Diabetic nephropathy; Glucose transporter proteins; Hypertension; Streptozotocin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100004

A novel homozygous mutation in the solute carrier family 12 member 3 gene in a Chinese family with Gitelman syndrome
Provedor de dados: 56
Autores: Zhang,Y.; Zhang,F.; Chen,D.; Lü,Q.; Tang,L.; Yang,C.; Lei,M.; Tong,N.
Loss of function of mutated solute carrier family 12 member 3 (SLC12A3) gene is the most frequent etiology for Gitelman syndrome (GS), which is mainly manifested by hypokalemia, hypomagnesemia and hypocalciuria. We report the genetic characteristics of one suspicious Chinese GS pedigree by gene sequencing. Complete sequencing analysis of the SLC12A3 gene revealed that both the proband and his elder sister had a novel homozygous SLC12A3 mutation: c.2099T&gt;C and p.Leu700Pro. Moreover, the SLC12A3 genes of his mother and daughter encoded the same mutated heterozygote. It was noted that in this pedigree, only the proband complained about recurrent episodes of bilateral lower limb weakness over 8 years, while his elder sister, mother and daughter did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gitelman syndrome; SLC12A3 gene; Homozygous mutant; Pedigree.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100701

Multiple factor analysis of metachronous upper urinary tract transitional cell carcinoma after radical cystectomy
Provedor de dados: 56
Autores: Wang,P.; Luo,J.D.; Wu,W.F.; Wang,S.; Cai,S.L.; Shen,B.H.; Shi,S.F.; Wei,K.X.; Zhang,Z.G.; Chen,Z.D.
Transitional cell carcinoma (TCC) of the urothelium is often multifocal and subsequent tumors may occur anywhere in the urinary tract after the treatment of a primary carcinoma. Patients initially presenting a bladder cancer are at significant risk of developing metachronous tumors in the upper urinary tract (UUT). We evaluated the prognostic factors of primary invasive bladder cancer that may predict a metachronous UUT TCC after radical cystectomy. The records of 476 patients who underwent radical cystectomy for primary invasive bladder TCC from 1989 to 2001 were reviewed retrospectively. The prognostic factors of UUT TCC were determined by multivariate analysis using the COX proportional hazards regression model. Kaplan-Meier analysis was also used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bladder neoplasm; Transitional cell carcinoma; Upper urinary tract; Radical cystectomy; Prognosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700013

The hydrogen sulfide donor, Lawesson's reagent, prevents alendronate-induced gastric damage in rats
Provedor de dados: 56
Autores: Nicolau,L.A.D.; Silva,R.O.; Damasceno,S.R.B.; Carvalho,N.S.; Costa,N.R.D.; Aragão,K.S.; Barbosa,A.L.R.; Soares,P.M.G.; Souza,M.H.L.P.; Medeiros,J.V.R.
Our objective was to investigate the protective effect of Lawesson's reagent, an H2S donor, against alendronate (ALD)-induced gastric damage in rats. Rats were pretreated with saline or Lawesson's reagent (3, 9, or 27 µmol/kg, po) once daily for 4 days. After 30 min, gastric damage was induced by ALD (30 mg/kg) administration by gavage. On the last day of treatment, the animals were killed 4 h after ALD administration. Gastric lesions were measured using a computer planimetry program, and gastric corpus pieces were assayed for malondialdehyde (MDA), glutathione (GSH), proinflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1β], and myeloperoxidase (MPO). Other groups were pretreated with glibenclamide (5 mg/kg, ip) or with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alendronate; Lawesson's reagent; H2S donors; Gastric damage.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800708

Hepatitis B virus genotype E detected in Brazil in an African patient who is a frequent traveler
Provedor de dados: 56
Autores: Sitnik,R; Sette Jr,H; Santana,R.A.F; Menezes,L.C; Graça,C.H.N; Dastoli,G.T.F; Silbert,S; Pinho,J.R.R.
Genotype E of hepatitis B virus (HBV) has not been described in Brazil and is found mainly in Africa. Genotype A is the most prevalent in Brazil, and genotypes B, C, D, and F have already been reported. We report here an HBV genotype E-infected patient and some characterization of surface (S) protein, DNA polymerase (P) and precore/core (preC/C) coding regions based on the viral genome. The patient is a 31-year-old black man with chronic hepatitis B who was born and raised in Angola. He has been followed by a hepatologist in São Paulo, Brazil, since November 2003, and he is a frequent traveler to Latin America, Africa, and Europe. In 2003, he was diagnosed with HBV infection and started treatment with lamivudine with the later addition of adefovir...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis B; HBV genotype E; Precore mutation; Brazil; Africa; African traveler.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200013

Synthesis and anti-myocarditis activity in a multifunctional lanthanide microporous metal-organic framework with 1D helical chain building units
Provedor de dados: 56
Autores: Hong,Chenglv; Zhou,Xinlang; Huang,Weijian; Shan,Peiren; Dong,Fengquan.
A new microporous lanthanide metal-organic framework, {[Yb(BTB)(H2O) (DEF)2}n (1, DEF=N,N-Diethylformamide), with 1D nano-sized channels has been constructed by bridging helical chain secondary building units with 1,3,5-benzenetrisbenzoic acid (H3BTB) ligand. Structural characterization suggests that this complex crystallizes in the hexagonal space group P6122 and possesses 1D triangular channels with coordinated water molecules pointing to the channel center. In addition, anti-myocarditis properties of compound 1 were evaluated in vivo. The results showed that compound 1 can improve hemodynamic parameters of, and it may be a good therapeutic option for heart failure in the future.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metal-organic framework; Anti-myocarditis; In vivo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300604

Tissue fusion technology versus suture and staple in porcine bowel anastomosis: an in vivo study
Provedor de dados: 56
Autores: Pan,Hong; Leung,Kevin K.C.; Ng,Enders Kwok Wai.
The aim of this study was to make a comparison between the tissue fusion technique and conventional methods for sealing bowel anastomosis. Eighteen female domestic pigs (Suidae, Sus) were used in our study. Tissue-fused anastomoses (LigaSure groups) were made in 13 animals (5 anastomoses per animal), which were subdivided into 4 groups according to different manufacturing settings: “LigaSure-L-1” and “LigaSure-L-2”, with low energy output level with 1 or 2 device-activated tissue sealing times, and “LigaSure-M” and “LigaSure-H”, with medium or high energy output level. As controls, automatically stapled (GIA group) and hand-sewn (suture group) anastomoses were utilized in 3 and 2 animals, respectively. There was no statistical difference in the overall...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bowel anastomosis; Tissue fusion technology; Porcine; In vivo; Suture; Staple.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500605

Effects of sirolimus alone or in combination with cyclosporine A on renal ischemia/reperfusion injury
Provedor de dados: 56
Autores: Pereira,B.J.; Castro,I.; Burdmann,E.A.; Malheiros,D.M.A.; Yu,L.
Calcineurin inhibitors exacerbate ischemic injury in transplanted kidneys, but it is not known if sirolimus protects or exacerbates the transplanted kidney from ischemic injury. We determined the effects of sirolimus alone or in combination with cyclosporin A (CsA) on oxygenated and hypoxic/reoxygenated rat proximal tubules in the following in vitro groups containing 6-9 rats per group: sirolimus (10, 50, 100, 250, 500, and 1000 ηg/mL); CsA (100 µg/mL); sirolimus (50 and 250 ηg/mL) + CsA (100 µg/mL); control; vehicle (20% ethanol). For in vivo studies, 3-week-old Wistar rats (150-250 g) were submitted to left nephrectomy and 30-min renal artery clamping. Renal function and histological evaluation were performed 24 h and 7 days after ischemia (I) in five...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclosporin A; Nephrotoxicity; Renal proximal tubules; Renal ischemia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800007

A strategy for obtaining social benefits from the gene revolution
Provedor de dados: 56
Autores: Castro,L.A.B. de.
The strategy described in the present paper offers details about the possibility for Brazil to play a more substantial role in the gene revolution. If successfully applied, the powerful science-based technology currently available in Brazil can contribute to extend the benefits of the gene revolution to the poorest countries, very much like the Green Revolution did in the past, thereby reducing the hunger syndrome which claimed the lives of millions of people in some Asian countries, particularly Pakistan and India, decades ago. In his visit to Brazil in February 2004, Norman Borlaug had the opportunity to witness the success of Brazilian agriculture. At a Conference held at ESALQ - Superior School of Agriculture Luiz de Queiroz in Piracicaba, SP, Brazil,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gene revolution; Large scale monitoring of 'genetically modified plants; Intellectual Property Rights (IPR); Patent and plant breeding laws; Foundation seed programs.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000001

Evaluation of the immune humoral response of Brazilian patients with Rubinstein-Taybi syndrome
Provedor de dados: 56
Autores: Sugayama,S.M.M.; Arslanian,C.; Sales,M.M.; Carneiro-Sampaio,M.
Rubinstein-Taybi syndrome (RTS) is a rare developmental disorder characterized by craniofacial dysmorphisms, broad thumbs and toes, mental and growth deficiency, and recurrent respiratory infections. RTS has been associated with CREBBP gene mutations, but EP300 gene mutations have recently been reported in 6 individuals. In the present study, the humoral immune response in 16 RTS patients with recurrent respiratory infections of possible bacterial etiology was evaluated. No significant differences between patients and 16 healthy controls were detected to explain the high susceptibility to respiratory infections: normal or elevated serum immunoglobulin levels, normal salivary IgA levels, and a good antibody response to both polysaccharide and protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rubinstein-Taybi syndrome; High IgM levels; B lymphocyte; Antibody response.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200011

Human papillomavirus infection and its association with cervical dysplasia in Ecuadorian women attending a private cancer screening clinic
Provedor de dados: 56
Autores: Brown,C.R.; Leon,M.L.; Muñoz,K.; Fagioni,A.; Amador,L.G.; Frain,B.; Tu,W.; Qadadri,B.; Brown,D.R.
Women living in Latin American countries bear a disproportionate burden of cervical cancer, a condition caused by infection with the human papillomavirus (HPV). We performed a study in Santa Elena, Guayas (currently Santa Elena Province), Ecuador, to determine how often HPV could be detected in women attending a private cancer screening clinic. Participants underwent a Pap test, and vaginal and cervical swabs were performed for HPV testing by the polymerase chain reaction (PCR). Each participant completed a verbally administered survey. The mean age of 302 participants was 37.7 years (range 18 to 78 years). The majority of cervical and vaginal specimens contained sufficient DNA to perform PCR. Overall, 24.2% of the participants had either a cervical or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical dysplasia; Ecuador.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700007

Galantamine protects against lipopolysaccharide-induced acute lung injury in rats
Provedor de dados: 56
Autores: Li,G.; Zhou,CL.; Zhou,QS.; Zou,HD.
Lipopolysaccharide (LPS)-induced endotoxemia triggers the secretion of proinflammatory cytokines and can cause acute lung injury (ALI). The high mobility group box 1 (HMGB1) protein plays an important role as a late mediator of sepsis and ALI. Galantamine (GAL) is a central acetylcholinesterase inhibitor that inhibits the expression of HMGB1. This study evaluated the effects of GAL by measuring levels of inflammatory mediators and observing histopathological features associated with LPS-induced ALI. Sixty 8-10 week old male Sprague-Dawley rats (200-240 g) were randomized into three groups as follows: control group, LPS group (7.5 mg/kg LPS), and LPS+GAL group (5 mg/kg GAL before LPS administration). Histopathological examination of lung specimens obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galantamine; Acute lung injury; Lipopolysaccharide; HMGB1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200601

Transient elastography and APRI score: looking at false positives and false negatives. Diagnostic performance and association to fibrosis staging in chronic hepatitis C
Provedor de dados: 56
Autores: Mendes,L.C.; Ferreira,P.A.; Miotto,N.; Zanaga,L.; Gonçales,E.; Lazarini,M.S.; Gonçales Júnior,F.L.; Stucchi,R.S.B.; Vigani,A.G.
Although long regarded as the gold standard for liver fibrosis staging in chronic hepatitis C (CHC), liver biopsy (LB) implies both the risk of an invasive procedure and significant variability. The aim of this study was to evaluate the diagnostic performance for transient elastography (TE) and aspartate aminotransferase to platelet index (APRI) used alone and in combination compared to liver biopsy and to analyze false positive/negative results. Patients with CHC, and no previous clinical diagnosis of cirrhosis were enrolled to undergo liver biopsy, TE and APRI. A total of 182 adult patients with a median age of 55 years and median body mass index of 26.71 kg/m2 were analyzed. On LB, 56% of patients had significant levels of fibrosis (METAVIR F≥2) and 28%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver biopsy; Noninvasive tests; APRI; Transient elastography; Accuracy; False results.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900702

Association of hepatic nuclear factor-4 in the apolipoprotein B promoter: a preliminary report
Provedor de dados: 56
Autores: Nóvak,E.M.; Dantas,K.C.; Charbel,C.E.; Bydlowski,S.P.
Previous studies have examined the arrangement of regulatory elements along the apolipoprotein B (apoB) promoter region (-3067 to +940) and a promoter fragment extending from nucleotides -150 to +124 has been demonstrated to be essential for transcriptional activation of the apoB gene in hepatic and intestinal cells. It has also been shown that transcriptional activation of apoB requires a synergistic interaction between hepatic nuclear factor-4 (HNF-4) and CCAAT/enhancer-binding protein a (C/EBPa) transcription factors. Here, we have examined the hypothesis that HNF-4 factor binding to DNA may induce a DNA helix bend, thus facilitating the communication with a C/EBPa factor located one helix turn from this HNF-4 factor in the apoB promoter. A gel...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Apolipoprotein B promoter; HNF-4; C/EBPa.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100006

Retraction notice for: “Ginsenoside Rd inhibits IL-1β-induced inflammation and degradation of intervertebral disc chondrocytes by increasing IL1RAP ubiquitination” [Braz J Med Biol Res (2019) 52(9): e8525]
Provedor de dados: 56
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200951

Antioxidant activity of the microalga Spirulina maxima
Provedor de dados: 56
Autores: Miranda,M.S.; Cintra,R.G.; Barros,S.B.M.; Mancini-Filho,J.
Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients. Spirulina contains phenolic acids, tocopherols and ß-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated with and without the extract at 37oC. The IC50 (concentration which causes a 50% reduction of oxidation) of the extract in this system was 0.18 mg/ml. The in vivo antioxidant capacity was evaluated in plasma and liver of animals receiving a daily dose of 5 mg for 2 and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Microalgae antioxidant activity Spirulina maxima phenolic compounds ß-carotene &lt; FONT FACE=Symbol&gt; A&lt; /FONT&gt; -tocopherol.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800007

Commentary on the IV São Paulo Research Conference: Cancer Today: from molecular biology to treatment, São Paulo, SP, Brazil, November 10-12, 2005
Provedor de dados: 56
Autores: Mihich,E.
Tipo: Info:eu-repo/semantics/report
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000001

The use of reverse transcription-PCR for the diagnosis of X-linked chronic granulomatous disease
Provedor de dados: 56
Autores: Agudelo-Flórez,P.; López,J.A.; Redher,J.; Carneiro-Sampaio,M.M.S.; Costa-Carvalho,B.T.; Grumach,A.S.; Condino-Neto,A.
Chronic granulomatous disease (CGD) is an inherited disorder of the innate immune system characterized by a defective oxidative burst of phagocytes and subsequent impairment of their microbicidal activity. Mutations in one of the NADPH-oxidase components affect gene expression or function of this system, leading to the phenotype of CGD. Defects in gp91-phox lead to X-linked CGD, responsible for approximately 70% of CGD cases. Investigation of the highly heterogeneous genotype of CGD patients includes mutation analysis, Northern blot or Western blot assays according to the particular case. The aim of the present study was to use reverse transcription (RT)-PCR for the analysis of molecular defects responsible for X-linked CGD in eight Brazilian patients and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide; Phagocytes; Primary immunodeficiency; Respiratory burst; Neutrophils; Human.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500001

Lung hyperinflation stimulates the release of inflammatory mediators in spontaneously breathing subjects
Provedor de dados: 56
Autores: Malbouisson,L.M.S.; Szeles,T.F.; Barbalho,L.; Massoco,C.O.; Carmona,M.J.C.; Carvalho,C.R.R.; Pelosi,P.; Auler Jr.,J.O.C.
Lung hyperinflation up to vital capacity is used to re-expand collapsed lung areas and to improve gas exchange during general anesthesia. However, it may induce inflammation in normal lungs. The objective of this study was to evaluate the effects of a lung hyperinflation maneuver (LHM) on plasma cytokine release in 10 healthy subjects (age: 26.1 ± 1.2 years, BMI: 23.8 ± 3.6 kg/m²). LHM was performed applying continuous positive airway pressure (CPAP) with a face mask, increased by 3-cmH2O steps up to 20 cmH2O every 5 breaths. At CPAP 20 cmH2O, an inspiratory pressure of 20 cmH2O above CPAP was applied, reaching an airway pressure of 40 cmH2O for 10 breaths. CPAP was then decreased stepwise. Blood samples were collected before and 2 and 12 h after LHM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung hyperinflation; Ventilator-induced lung injury; Inflammation; Cytokines.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200011

Antiprotozoal and molluscicidal activities of five Brazilian plants
Provedor de dados: 56
Autores: Truiti,M.C.T.; Ferreira,I.C.P.; Zamuner,M.L.M.; Nakamura,C.V.; Sarragiotto,M.H.; Souza,M.C.
Leishmaniasis, Chagas' disease and schistosomiasis (bilharzia) are parasitic diseases with wide distribution on the American continent, affecting millions of people. In the present study, biological assays for antiprotozoal and molluscicidal activities were carried out with ethanolic extracts of plant species from the Brazilian part of the Upper Paraná River. Crude extracts were obtained by percolation with absolute ethanol from the leaves of Cayaponia podantha Cogn., Nectandra falcifolia (Nees) Castiglioni and Paullinia elegans Cambess., as well as from the aerial parts of Helicteres gardneriana St. Hil. &amp;amp; Naud. and Melochia arenosa Benth., all belonging to genera used in folk medicine. Trypanocidal activity of plants was assayed on epimastigote...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cayaponia podantha; Helicteres gardneriana; Melochia arenosa; Nectandra falcifolia; Paullinia elegans; Antiprotozoal activity; Molluscicidal activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200016

NOP14 inhibits melanoma proliferation and metastasis by regulating Wnt/β-catenin signaling pathway
Provedor de dados: 56
Autores: Li,Jingrong; Fang,Ruihua; Wang,Jianqin; Deng,Liehua.
Malignant melanoma is an aggressive skin cancer with a high mortality rate. Nucleolar protein 14 (NOP14) has been implicated in cancer development. However, the role of NOP14 in malignant melanoma progression remains largely unclear. In this study, we observed that malignant melanoma tissue showed NOP14 down-regulation compared to melanocytic nevi tissues. Moreover, we observed that NOP14 expression was significantly associated with melanoma tumor thickness and lymph node metastasis. NOP14 overexpression in melanoma cells suppressed proliferation, caused G1 phase arrest, promoted apoptosis, and inhibited melanoma cell migration and invasion. Further investigations revealed that NOP14 overexpression reduced the expression levels of Wnt3a, β-catenin, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant melanoma; Metastasis; NOP14; Proliferation; Wnt/β-catenin pathway.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100604

PCR-based ribotyping of Staphylococcus aureus
Provedor de dados: 56
Autores: Oliveira,A.M.; Ramos,M.C.
Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for genotyping purpose, by PCR-based ribotyping. Three primer pairs were tested to determine the size of amplicons produced and to obtain better discrimination with agar gel electrophoresis and ethidium bromide staining. The resolution of the typing system was determined using sets of bacteria obtained from clinical specimens from a large tertiary care hospital. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; PCR ribotyping; Genotyping; Methicillin-resistant Staphylococcus aureus.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200005

MALAT1 is involved in type I IFNs-mediated systemic lupus erythematosus by up-regulating OAS2, OAS3, and OASL
Provedor de dados: 56
Autores: Gao,Fei; Tan,Yuan; Luo,Hong.
Systemic lupus erythematosus (SLE) is an autoimmune disease associated with an aberrant activation of immune cells partly due to the dysfunction of cytokines such as type I interferons (IFNs). Long non-coding RNA MALAT1 has been found to play a pathogenic role in SLE; however, the underlying mechanisms are still poorly understood. Bioinformatics analysis showed the up-regulation of type I IFN downstream effectors OAS2, OAS3, and OASL (OAS-like) in CD4+ T cells, CD19+ B cells, and CD33+ myeloid cells in patients with active SLE compared to healthy participants. In this study, peripheral blood mononuclear cells (PBMCs), CD19+ B, and CD4+ T cells were isolated from active SLE patients and healthy participants. PCR was performed to quantify MALAT1, OAS2, OAS3,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MALAT1; OAS2; OAS3; OASL; Systemic lupus erythematosus; Type I IFNs.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500603

Identification of a novel UMOD mutation (c.163G&amp;gt;A) in a Brazilian family with autosomal dominant tubulointerstitial kidney disease
Provedor de dados: 56
Autores: Lopes,L.B.; Abreu,C.C.; Souza,C.F.; Guimaraes,L.E.R.; Silva,A.A.; Aguiar-Alves,F.; Kidd,K.O.; Kmoch,S.; Bleyer,A.J.; Almeida,J.R.
Autosomal dominant tubulointerstitial kidney disease (ADTKD) is characterized by autosomal dominant inheritance, progressive chronic kidney disease, and a bland urinary sediment. ADTKD is most commonly caused by mutations in the UMOD gene encoding uromodulin (ADTKD-UMOD). We herein report the first confirmed case of a multi-generational Brazilian family with ADTKD-UMOD, caused by a novel heterozygous mutation (c.163G&gt;A, GGC→AGC, p.Gly55Ser) in the UMOD gene. Of 41 family members, 22 underwent genetic analysis, with 11 individuals found to have this mutation. Three affected individuals underwent hemodialysis, one peritoneal dialysis, and one patient received a kidney transplant from a family member later found to be genetically affected. Several younger...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney disease; Uromodulin kidney disease; Genetic mutation; Uromodulin; UMOD; Genetic kidney diseases.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300616

Gene expression of estrogen and oxytocin receptors in the uterus of pregnant and parturient bitches
Provedor de dados: 56
Autores: Veiga,G.A.L.; Milazzotto,M.P.; Nichi,M.; Lúcio,C.F.; Silva,L.C.G.; Angrimani,D.S.R.; Vannucchi,C.I.
In the canine species, the precise mechanisms of pregnancy maintenance and the initiation of parturition are not completely understood. The expression of genes encoding the receptors for estrogen (ERα mRNA) and oxytocin (OTR mRNA) was studied in the endometrium and myometrium during pregnancy and parturition in dogs. Real-time PCR was performed to quantify the levels of ERα mRNA and OTR mRNA in the uterus of bitches during early (up to 20 days of gestation), mid (20 to 40 days) and late pregnancy (41 to 60 days), and parturition (first stage of labor). All tissues expressed ERα and OTR mRNA, and are thus possibly able to respond to eventual estrogen and oxytocin hormonal stimuli. No statistically significant differences in the expression of ERα mRNA were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine; Estrogen; Oxytocin; Receptors; Pregnancy; Parturition.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400339

Correlation between sodium and potassium excretion in 24- and 12-h urine samples
Provedor de dados: 56
Autores: Mill,J.G.; Silva,A.B.T. da; Baldo,M.P.; Molina,M.C.B.; Rodrigues,S.L.
Low-sodium and high-potassium diets have been recommended as an adjunct to prevention and treatment of hypertension. Analysis of these nutrients in 24-h urine has been considered the reference method to estimate daily intake of these minerals. However, 24-h urine collection is difficult in epidemiological studies, since urine must be collected and stored in job environments. Therefore, strategies for shorter durations of urine collection at home have been proposed. We have previously reported that collecting urine during a 12-h period (overnight) is more feasible and that creatinine clearance correlated strongly with that detected in 24-h samples. In the present study, we collected urine for 24 h divided into two 12-h periods (from 7:00 am to 7:00 pm and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sodium excretion; Urine; Salt intake; Potassium excretion.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900002

Identification of clonally rearranged T-cell receptor beta chain genes in HTLV-I carriers as a potential instrument for early detection of neoplasia
Provedor de dados: 56
Autores: Sales,M.M.; Bezerra,C.N.A.; Hiraki,Y.; Melo,N.B.; Rebouças,N.A.
We analyzed the genetic recombination pattern of the T-cell receptor beta-chain gene (TCR-beta) in order to identify clonal expansion of T-lymphocytes in 17 human T-lymphotropic virus type I (HTLV-I)-positive healthy carriers, 7 of them with abnormal features in the peripheral blood lymphocytes. Monoclonal or oligoclonal expansion of T-cells was detected in 5 of 7 HTLV-I-positive patients with abnormal lymphocytes and unconfirmed diagnosis by using PCR amplification of segments of TCR-beta gene, in a set of reactions that target 102 different variable (V) segments, covering all members of the 24 V families available in the gene bank, including the more recently identified segments of the Vbeta-5 and Vbeta-8 family and the two diversity beta segments....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human T-lymphotropic virus 1; Leukemia; Oligoclonal bands; T-cell receptor beta chain; Polymerase chain; Reaction; Adult T-cell leukemia/lymphoma.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500006

Glucocorticoids, master modulators of the thymic catecholaminergic system?
Provedor de dados: 56
Autores: Pilipović,I.; Kosec,D.; Radojević,K.; Perišić,M.; Pešić,V.; Stojić-Vukanić,Z.; Leposavi,G.
There is evidence that the major mediators of stress, i.e., catecholamines and glucocorticoids, play an important role in modulating thymopoiesis and consequently immune responses. Furthermore, there are data suggesting that glucocorticoids influence catecholamine action. Therefore, to assess the putative relevance of glucocorticoid-catecholamine interplay in the modulation of thymopoiesis we analyzed thymocyte differentiation/maturation in non-adrenalectomized and andrenalectomized rats subjected to treatment with propranolol (0.4 mg·100 g body weight-1·day-1) for 4 days. The effects of β-adrenoceptor blockade on thymopoiesis in non-adrenalectomized rats differed not only quantitatively but also qualitatively from those in adrenalectomized rats. In...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Catecholamines; Glucocorticoids; Β-adrenoceptors; Propranolol; Thymocyte apoptosis; Thymocyte proliferation; Thymocyte differentiation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300009

The hematopoietic stroma
Provedor de dados: 56
Autores: Nardi,N.B.; Alfonso,Z.Z.C.
All blood cells are derived from a small common pool of totipotent cells, called hematopoietic stem cells. The process is strictly regulated by the hematopoietic microenvironment, which includes stromal cells, extracellular matrix molecules and soluble regulatory factors. Several experimental in vitro assays have been developed for the study of hematopoietic differentiation, and have provided valuable information on the stroma, which includes, among other cell types, macrophages, fibroblasts, adipocytes, and endothelial cells. The composition, ontogeny, and function in physiological as well as pathological conditions of stroma are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoiesis; Microenvironment; Stroma.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500014

Resveratrol inhibits the intracellular calcium increase and angiotensin/endothelin system activation induced by soluble uric acid in mesangial cells
Provedor de dados: 56
Autores: Albertoni,G.; Schor,N.
Resveratrol (Resv) is natural polyphenol found in grapes. This study evaluated the protective effect of Resv against the effects of uric acid (UA) in immortalized human mesangial cells (ihMCs). ihMCs were preincubated with Resv (12.5 µM) for 1 h and treated with UA (10 mg/dL) for 6 or 12 h. The intracellular calcium concentration [Ca2+]i was quantified by fluorescence using flow cytometry. Angiotensinogen (AGT) and pre-pro endothelin-1 (ppET-1) mRNA were assayed by quantitative real-time RT-PCR. Angiotensin II (AII) and endothelin-1 (ET-1) were assayed by ELISA. UA significantly increased [Ca2+]i. Pre-incubation with Resv significantly reduced the change in [Ca2+]i induced by UA. Incubation with UA for 6 or 12 h also increased AGT mRNA expression and AII...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resveratrol; Intracellular calcium; Angiotensin; Endothelin; Mesangial cells; Uric acid.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100051

Synaptic vesicle pool size, release probability and synaptic depression are sensitive to Ca2+ buffering capacity in the developing rat calyx of Held
Provedor de dados: 56
Autores: Leão,R.M.; von Gersdorff,H.
The calyx of Held, a specialized synaptic terminal in the medial nucleus of the trapezoid body, undergoes a series of changes during postnatal development that prepares this synapse for reliable high frequency firing. These changes reduce short-term synaptic depression during tetanic stimulation and thereby prevent action potential failures during a stimulus train. We measured presynaptic membrane capacitance changes in calyces from young postnatal day 5-7 (p5-7) or older (p10-12) rat pups to examine the effect of calcium buffer capacity on vesicle pool size and the efficiency of exocytosis. Vesicle pool size was sensitive to the choice and concentration of exogenous Ca2+ buffer, and this sensitivity was much stronger in younger animals. Pool size and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calyx of Held; Neurotransmission; Calcium buffer; Capacitance measurements; Mammalian auditory brainstem; Synaptic development.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100014

HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
Provedor de dados: 56
Autores: Abba,M.C.; Gómez,M.A.; Golijow,C.D.
In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a small native polyacrylamide gel, followed by a rapid silver staining procedure. In order to validate the technique and to obtain the allele patterns for the DQA1 gene, 50 cervical samples were typed using this methodology and the commercial Amplitype® HLA DQA1 Amplification and Typing kit. All the alleles detected with the kit were characterized by the LIS-SSCP approach....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HLA-DQA1; PCR; LIS-SSCP.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700005

alpha-Smooth muscle actin and proliferating cell nuclear antigen expression in focal segmental glomerulosclerosis: functional and structural parameters of renal disease progression
Provedor de dados: 56
Autores: Geleilete,T.J.M.; Costa,R.S.; Dantas,M.; Coimbra,T.M.
The aim of the present study was to investigate the expression of alpha-smooth muscle actin (alpha-SM-actin) and proliferating cell nuclear antigen (PCNA) in renal cortex from patients with focal segmental glomerulosclerosis (FSGS) and their correlations with parameters of renal disease progression. We analyzed renal biopsies from 41 patients with idiopathic FSGS and from 14 control individuals. The alpha-SM-actin immunoreaction was evaluated using a score that reflected the changes in the extent and intensity of staining in the glomerular or cortical area. The PCNA reaction was quantified by counting the labeled cells of the glomeruli or renal cortex. The results, reported as median ± percentile (25th; 75th), showed that the alpha-SM-actin scores in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-smooth muscle actin; Proliferating cell nuclear antigen; PCNA; Focal segmental glomerulosclerosis; Proteinuria; Renal failure; Renal disease progression; Renal fibrosis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800003

The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells
Provedor de dados: 56
Autores: Chen,W.L.; Luo,D.F.; Gao,C.; Ding,Y.; Wang,S.Y.
The familial acute myeloid leukemia related factor gene (FAMLF) was previously identified from a familial AML subtractive cDNA library and shown to undergo alternative splicing. This study used real-time quantitative PCR to investigate the expression of the FAMLF alternative-splicing transcript consensus sequence (FAMLF-CS) in peripheral blood mononuclear cells (PBMCs) from 119 patients with de novo acute leukemia (AL) and 104 healthy controls, as well as in CD34+ cells from 12 AL patients and 10 healthy donors. A 429-bp fragment from a novel splicing variant of FAMLF was obtained, and a 363-bp consensus sequence was targeted to quantify total FAMLF expression. Kruskal-Wallis, Nemenyi, Spearman's correlation, and Mann-Whitney U-tests were used to analyze...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FAMLF; Gene expression; Leukemia; Real-time polymerase chain reaction; Alternative splicing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700603

Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
Provedor de dados: 56
Autores: Chen,Yan; Zheng,Yu; Zhou,Zhihong; Wang,Jinjun.
Tubular-interstitial nephritis (TIN) is characterized by tubular cell damage and inflammatory lesions of kidneys. Baicalein (BAI) is a flavonoid compound found in the roots of Scutellaria baicalensis Georgi. The present study was undertaken to explore the anti-inflammatory and anti-oxidative effects of BAI on TIN patients and a lipopolysaccharide (LPS)-induced TIN cell model. The expression levels of interleukin-6 (IL-6), IL-10, and tumor necrosis factor α in serum samples of TIN patients and culture supernatants of renal proximal tubular epithelial cells (RPTECs) were evaluated using enzyme-linked immunosorbent assay. Creatinine clearance was calculated using the Cockcroft-Gault equation. Activities of malondialdehyde, superoxide dismutase, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tubular-interstitial nephritis; Baicalein; Lipopolysaccharide; Anti-inflammatory effects; NF-κB pathway; MAPK pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000606

Resistance exercise improves hippocampus-dependent memory
Provedor de dados: 56
Autores: Cassilhas,R.C.; Lee,K.S.; Venâncio,D.P.; Oliveira,M.G.M.; Tufik,S.; Mello,M.T.
It has been demonstrated that resistance exercise improves cognitive functions in humans. Thus, an animal model that mimics this phenomenon can be an important tool for studying the underlying neurophysiological mechanisms. Here, we tested if an animal model for resistance exercise was able to improve the performance in a hippocampus-dependent memory task. In addition, we also evaluated the level of insulin-like growth factor 1/insulin growth factor receptor (IGF-1/IGF-1R), which plays pleiotropic roles in the nervous system. Adult male Wistar rats were divided into three groups (N = 10 for each group): control, SHAM, and resistance exercise (RES). The RES group was submitted to 8 weeks of progressive resistance exercise in a vertical ladder apparatus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical exercise; Passive avoidance; Insulin-like growth factor 1; Resistance training; Hippocampus; Memory.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200015

Comparison of I-FISH and G-banding for the detection of chromosomal abnormalities during the evolution of myelodysplastic syndrome
Provedor de dados: 56
Autores: Pinheiro,R.F.; Chauffaille,M.L.L.F.
Myelodysplastic syndrome (MDS) patients with a normal karyotype constitute a heterogeneous group from a biological standpoint and their outcome is often unpredictable. Interphase fluorescence in situ hybridization (I-FISH) studies could increase the rate of detection of abnormalities, but previous reports in the literature have been contradictory. We performed I-FISH and conventional karyotyping (G-banding) on 50 MDS patients at diagnosis, after 6 and 12 months or at any time if a transformation to acute myeloid leukemia (AML) was detected. Applying a probe-panel targeting the centromere of chromosomes 7 and 8, 5q31, 5p15.2 and 7q31, we observed one case with 5q deletion not identified by G-banding. I-FISH at 6 and 12 months confirmed the karyotype...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myelodysplastic syndrome; FISH; G-banding.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100018

Modification of pyruvate kinase and lactate dehydrogenase in foot muscle of the sea mussel Mytilus galloprovincialis under anaerobiosis and recovery
Provedor de dados: 56
The modification of pyruvate kinase (PK) and lactate dehydrogenase (LDH) activity in foot muscle of the mussel Mytilus galloprovincialis during exposure to air and recovery in water was investigated. In the course of exposure to air, the activity of these enzymes measured at high and low substrate concentrations showed successive increases and decreases. Returning the mussels to water after exposure to air affected enzyme activity in a manner similar to anaerobiosis. When measuring at saturated concentrations of substrates and substrate and coenzyme for PK and LDH, respectively, the maximum activation of PK (37%) was observed at 4 h of animal exposure to air, and for LDH (67%) at 6 h exposure to air. During 24 h of exposure of animals to air, PK activity...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mytilus galloprovincialis; Muscle; Anaerobiosis; Pyruvate kinase; Lactate dehydrogenase; Property modification.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300012

Prevalence of serological markers of hepatitis B virus in pregnant women from Paraná State, Brazil
Provedor de dados: 56
Autores: Bertolini,D.A.; Pinho,J.R.R.; Saraceni,C.P.; Moreira,R.C.; Granato,C.F.H.; Carrilho,F.J.
The prevalence of hepatitis B virus (HBV) in Brazil increases from South to North but moderate to elevated prevalence has been detected in the Southwest of Paraná State. The prevalence of serological markers of HBV was evaluated in 3188 pregnant women from different counties in Paraná State and relevant epidemiological features were described. The prevalence of HBV markers in pregnant women for the state as a whole was 18.5% (95% CI = 17.2-19.9), ranging from 7.2% in Curitiba to 38.5% in Francisco Beltrão. The endemicity of HBV marker prevalence in pregnant women was intermediate in Cascavel, Foz do Iguaçu, and Francisco Beltrão, and low in Curitiba, Londrina, Maringá, and Paranaguá. Multiple logistic regression showed that HBV marker prevalence increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B virus; Brazil; Serological markers; Pregnant women.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800011

Contrast sensitivity threshold measured by sweep-visual evoked potential in term and preterm infants at 3 and 10 months of age
Provedor de dados: 56
Autores: Oliveira,A.G.F.; Costa,M.F.; Souza,J.M. de; Ventura,D.F.
Although healthy preterm infants frequently seem to be more attentive to visual stimuli and to fix on them longer than full-term infants, no difference in visual acuity has been reported compared to term infants. We evaluated the contrast sensitivity (CS) function of term (N = 5) and healthy preterm (N = 11) infants at 3 and 10 months of life using sweep-visual evoked potentials. Two spatial frequencies were studied: low (0.2 cycles per degrees, cpd) and medium (4.0 cpd). The mean contrast sensitivity (expressed in percentage of contrast) of the preterm infants at 3 months was 55.4 for the low spatial frequency (0.2 cpd) and 43.4 for the medium spatial frequency (4.0 cpd). At 10 months the low spatial CS was 52.7 and the medium spatial CS was 9.9. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast sensitivity; Preterm infants; Sweep-visual evoked potential; Visual development; Visual acuity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900014

Structural basis for the pathophysiology of lipoprotein(a) in the athero-thrombotic process
Provedor de dados: 56
Autores: Anglés-Cano,E.
Lipoprotein Lp(a) is a major and independent genetic risk factor for atherosclerosis and cardiovascular disease. The essential difference between Lp(a) and low density lipoproteins (LDL) is apolipoprotein apo(a), a glycoprotein structurally similar to plasminogen, the precursor of plasmin, the fibrinolytic enzyme. This structural homology endows Lp(a) with the capacity to bind to fibrin and to membrane proteins of endothelial cells and monocytes, and thereby to inhibit plasminogen binding and plasmin generation. The inhibition of plasmin generation and the accumulation of Lp(a) on the surface of fibrin and cell membranes favor fibrin and cholesterol deposition at sites of vascular injury. Moreover, insufficient activation of TGF-ß due to low plasmin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Apolipoprotein(a); Thrombosis; Atheroma; Plasminogen; Lipoprotein; Fibrin; Fibrinolysis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100002

Reliability and clinical utility of a Portuguese version of the Abnormal Involuntary Movements Scale (AIMS) for tardive dyskinesia in Brazilian patients
Provedor de dados: 56
Autores: Tonelli,H.; Tonelli,D.; Poiani,G.R.; Vital,M.A.B.F.; Andreatini,R.
The objective of the present study was to evaluate the reliability and clinical utility of a Portuguese version of the Abnormal Involuntary Movements Scale (AIMS). Videotaped interviews with 16 psychiatric inpatients treated with antipsychotic drugs for at least 5 years were evaluated. Reliability was assessed by the intraclass correlation coefficient (ICC) between three raters, two with and one without clinical training in psychopathology. Clinical utility was assessed by the difference between the scores of patients with (N = 11) and without (N = 5) tardive dyskinesia (TD). Patients with TD exhibited a higher severity of global evaluation by the AIMS (sum of scores: 4.2 ± 0.9 vs 0.4 ± 0.2; score on item 8: 2.3 ± 0.3 vs 0.4 ± 0.2, TD vs controls). The ICC...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Reliability; Scale; Tardive dyskinesia; Clinical utility.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400014

Ventilation with high tidal volume induces inflammatory lung injury
Provedor de dados: 56
Autores: Bueno,P.C.S.; Bueno,C.E.; Santos,M.L.; Oliveira-Júnior,I.; Salomão,R.; Pinheiro,B.V.; Beppu,O.S.
Mechanical ventilation with high tidal volumes (V T) has been shown to induce lung injury. We examined the hypothesis that this procedure induces lung injury with inflammatory features. Anesthetized male Wistar rats were randomized into three groups: group 1 (N = 12): V T = 7 ml/kg, respiratory rate (RR) = 50 breaths/min; group 2 (N = 10): V T = 21 ml/kg, RR = 16 breaths/min; group 3 (N = 11): V T = 42 ml/kg, RR = 8 breaths/min. The animals were ventilated with fraction of inspired oxygen of 1 and positive end-expiratory pressure of 2 cmH2O. After 4 h of ventilation, group 3, compared to groups 1 and 2, had lower PaO2 [280 (range 73-458) vs 517 (range 307-596), and 547 mmHg (range 330-662), respectively, P&lt;0.05], higher wet lung weight [3.62 ± 0.91 vs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung injury; Mechanical ventilation; Inflammation; TNF-alpha.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200007

Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway
Provedor de dados: 56
Autores: Zhang,Lei-Yi; Zhou,Yue-Ying; Chen,Fei; Wang,Bing; Li,Jing; Deng,You-Wen; Liu,Wei-Dong; Wang,Zheng-Guang; Li,Ya-Wei; Li,Dong-Zhe; Lv,Guo-Hua; Yin,Bang-Liang.
Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.75% reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phosphorylation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taurine; Osteoblasts; Apoptosis; Taurine transporter; ERK.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700002

Effect of acute treatment with a water-alcohol extract of Erythrina mulungu on anxiety-related responses in rats
Provedor de dados: 56
Autores: Onusic,G.M.; Nogueira,R.L.; Pereira,A.M.S.; Viana,M.B.
We investigated the effect of acute oral treatment with a water-alcohol extract of the inflorescence of Erythrina mulungu (EM, Leguminosae-Papilionaceae) (100, 200 and 400 mg/kg) on rats submitted to different anxiety models: the elevated T-maze (for inhibitory avoidance and escape measurements), the light/dark transition, and the cat odor test. These models were selected for their presumed capacity to demonstrate specific subtypes of anxiety disorders as recognized in clinical practice. Treatment with 200 mg/kg EM impaired avoidance latencies (avoidance 1 - 200 mg/kg EM: 18 ± 7 s, control group: 40 ± 9 s; avoidance 2 - 200 mg/kg EM: 15 ± 4 s, control group: 110.33 ± 38 s) in a way similar to the reference drug diazepam (avoidance 1: 3 ± 0.79 s; avoidance...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Erythrina mulungu; Anxiety; Animal models.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400011

Low occurrence of occult hepatitis B virus infection and high frequency of hepatitis C virus genotype 3 in hepatocellular carcinoma in Brazil
Provedor de dados: 56
Autores: Alencar,R.S.M.; Gomes,M.M.S.; Sitnik,R.; Pinho,J.R.R.; Malta,F.M.; Mello,I.M.V.G.C.; Mello,E.S.; Bacchella,T.; Machado,M.C.C.; Alves,V.A.F.; Carrilho,F.J.
Occult hepatitis B virus (HBV) infection has been reported among patients with hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC). Our aim was to evaluate the presence of occult HBV infection in patients with HCV-related liver cirrhosis (LC) with or without HCC in São Paulo, Brazil. Serum and liver tissue samples from 50 hepatitis B surface antigen-negative patients with HCV-related LC who underwent liver transplantation at the University of São Paulo School of Medicine Hospital from 1993 to 2004 were divided into groups with LC only (N = 33) and with LC plus HCC (N = 17). HBV DNA was assayed for serum and paraffin-embedded liver tissue (tumoral and non-tumoral) using real time PCR and only 1 case with HCC had HBV DNA-positive serum. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Occult hepatitis B; Hepatocellular carcinoma; HCV genotypes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300010

Candesartan, rather than losartan, improves motor dysfunction in thioacetamide-induced chronic liver failure in rats
Provedor de dados: 56
Autores: Murad,H.A.; Gazzaz,Z.J.; Ali,S.S.; Ibraheem,M.S.
Minimal hepatic encephalopathy is more common than the acute syndrome. Losartan, the first angiotensin-II receptor blocker (ARB), and candesartan, another widely-used ARB, have protected against developing fibrogenesis, but there is no clear data about their curative antifibrotic effects. The current study was designed to examine their effects in an already-established model of hepatic fibrosis and also their effects on the associated motor dysfunction. Low-grade chronic liver failure (CLF) was induced in 3-month old Sprague-Dawley male rats using thioacetamide (TAA, 50 mg·kg−1·day−1) intraperitoneally for 2 weeks. The TAA-CLF rats were randomly divided into five groups (n=8) treated orally for 14 days (mg·kg−1·day−1) as follows: TAA (distilled water),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candesartan; Hepatic fibrosis; Liver failure; Losartan; Motor dysfunction; Thioacetamide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100611

Use of anti-PGL-1 antibodies to monitor therapy regimes in leprosy patients
Provedor de dados: 56
Autores: Zenha,E.M.R.; Ferreira,M.A.N.; Foss,N.T.
The suitability of IgM antibodies to PGL-1 for monitoring the response to multidrug therapy (MDT) was sequentially tested by ELISA in 105 leprosy patients, and bacterial indexes (BI) were also determined. Patients were divided into 3 groups: group 1, 34 multibacillary (MB) patients treated for 12 months with MDT-MB; group 2, 33 MB patients treated for 24 months with MDT-MB, and group 3, 38 paucibacillary (PB) patients treated for 6 months with MDT-PB. Untreated MB patients exhibited higher antibody levels (mean ± SEM): group 1 (6.95 ± 1.35) and group 2 (12.53 ± 2.02) than untreated PB patients (1.28 ± 0.35). There was a significant difference (P &lt; 0.01) in anti-PGL-1 levels in group 1 patients: untreated (6.95 ± 1.35) and treated for 12 months (2.78 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leprosy; Anti-PGL-1; Multidrug therapy; MDT 12 months; MDT 24 months.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000016

Intensity of swimming exercise influences aortic reactivity in rats
Provedor de dados: 56
Autores: Brito,A.F.; Silva,A.S.; Souza,I.L.L.; Pereira,J.C.; Silva,B.A. da.
Exercise is known to cause a vasodilatory response; however, the correlation between the vasorelaxant response and different training intensities has not been investigated. Therefore, this study evaluated the vascular reactivity and lipid peroxidation after different intensities of swimming exercise in rats. Male Wistar rats (aged 8 weeks; 250-300 g) underwent forced swimming for 1 h whilst tied to loads of 3, 4, 5, 6, and 8% of their body weight, respectively (groups G3, G4, G5, G6 and G8, respectively; n=5 each). Immediately after the test, the aorta was removed and suspended in an organ bath. Cumulative relaxation in response to acetylcholine (10−12-10−4 M) and contraction in response to phenylephrine (10−12-10−5 M) were measured. Oxidative stress was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aerobic exercise; Anaerobic exercise; Lipid peroxidation; Smooth muscle; Swimming; Vessel.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100996

Cardiovascular and respiratory changes during slow-wave sleep in rats are associated with electrocorticogram desynchronization
Provedor de dados: 56
Autores: Dias-dos-Santos,J.R.; Machado,B.H.
In awake rats a single recurrent larger tidal volume (deep breaths) occurs at regular intervals, followed by oscillations in arterial pressure and heart rate. In the present study we recorded the changes in blood pressure, heart rate and ventilation during the wakefulness-sleep cycle identified by electrocorticographic records in order to determine whether the deep breaths and cardiovascular oscillations were associated with changes in the electrocorticogram. During several episodes of slow-wave sleep (SWS) in 7 rats the deep breaths and oscillations in arterial pressure and heart rate were preceded by SWS desynchronization. The interval between deep breaths during SWS was 71 ± 4 s, the period between initial desynchronization and the generation of deep...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wakefulness-sleep cycle; Ventilation; Cardiovascular regulation; Deep breaths; Ventilatory patterns; Arterial pressure oscillations; Whole body plethysmography.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100018

Effects of tryptophan depletion on anxiety induced by simulated public speaking
Provedor de dados: 56
Autores: Monteiro-dos-Santos,P.C.; Graeff,F.G.; dos-Santos,J.E.; Ribeiro,R.P.P.; Guimarães,F.S.; Zuardi,A.W.
Several lines of evidence point to the participation of serotonin (5HT) in anxiety. Its specific role, however, remains obscure. The objective of the present study was to evaluate the effect of reducing 5HT-neurotransmission through an acute tryptophan depletion on anxiety induced by a simulated public speaking (SPS) test. Two groups of 14-15 subjects were submitted to a 24-h diet with a low or normal content of tryptophan and received an amino acid mixture without (TRY-) or with (TRY+) tryptophan under double-blind conditions. Five hours later they were submitted to the SPS test. The state-trait anxiety inventory (STAI) and the visual analogue mood scale (VAMS) were used to measure subjective anxiety. Both scales showed that SPS induced a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; Anxiety; Tryptophan depletion; Public speaking; Healthy volunteers; Gender differences.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500013

Aging and immunoglobulin isotype patterns in oral tolerance
Provedor de dados: 56
Autores: Faria,A.M.C.; Ficker,S.M.; Speziali,E.; Menezes,J.S.; Stransky,B.; Verdolin,B.A.; Lahmann,W.M.; Rodrigues,V.S.; Vaz,N.M.
In the present review we address oral tolerance as an important biological phenomenon and discuss how it is affected by aging. Other factors such as frequency of feeding and previous digestion of the antigen also seem to influence the establishment of oral tolerance. We also analyze immunoglobulin isotypes of specific antibodies formed by tolerant and immunized animals of different ages submitted to different conditions of oral antigen administration. Isotypic patterns were studied as a parameter for assessing the pathways of B and T cell interactions leading to antibody production
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral tolerance; Aging; Isotypes; Gut; Mucosa.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100005

Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil
Provedor de dados: 56
Autores: Sousa-Junior,F.C. de; Silva-Carvalho,M.C.; Fernandes,M.J.B.C.; Vieira,M.F.P.; Pellegrino,F.L.P.C.; Figueiredo,A.M.S.; Melo,M.C.N. de; Milan,E.P.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methicillin-resistant Staphylococcus aureus; MRSA; Antimicrobial resistance; Brazilian epidemic clone; Pediatric clone; SCCmecIV.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000002

Inhibition of miR-383 suppresses oxidative stress and improves endothelial function by increasing sirtuin 1
Provedor de dados: 56
Autores: Hu,Baoxiang; Gong,Zushun; Bi,Zhaohui.
Previous research has shown that suppression of miR-383 can prevent inflammation of the endothelium, as well as postpone the development of atherosclerosis. However, the role of miR-383 in endothelial cell apoptosis in diabetes remains unclear. The aim of this study was to investigate the function of miR-383 in high glucose-induced apoptosis and oxidative stress in endothelial cells. A series of experiments involving qualitative polymerase chain reaction, cell transfection, luciferase assay, assessment of cell death, detection of catalase and superoxide dismutase concentrations, detection of intracellular reactive oxygen species (ROS), and western blot analysis were performed in this study. We found that miR-383 expression was promoted, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-383; Oxidative stress; Human umbilical vein endothelial cells; Sirtuin 1.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200603

Advances in biliary atresia: from patient care to research
Provedor de dados: 56
Autores: Santos,J.L.; Carvalho,E.; Bezerra,J.A.
Biliary atresia, the most common cause of liver transplantation in children, remains a challenge for clinicians and investigators. The development of new therapeutic options, besides the typical hepatoportoenterostomy, depends on a greater understanding of its pathogenesis and how it relates to the clinical phenotypes at diagnosis and the rate of disease progression. In this review, we present a perspective of how recent research has advanced the understanding of the disease and has improved clinical care protocols. Molecular and morphological analyses at diagnosis point to the potential contributions of polymorphism in the CFC1 and VEGF genes to the pathogenesis of the disease, and to an association between the degree of bile duct proliferation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biliary atresia; Etiology; Prognosis; Therapeutics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600001

A review on edible vaccines and their prospects
Provedor de dados: 56
Autores: Gunasekaran,B.; Gothandam,K.M.
For a long time, vaccines have been the main mode of defense and protection against several bacterial, viral, and parasitic diseases. However, the process of production and purification makes them expensive and unaffordable to many developing nations. An edible vaccine is when the antigen is expressed in the edible part of the plant. This reduces the cost of production of the vaccine because of ease of culturing. In this article, various types of edible vaccines that include algal and probiotics in addition to plants are discussed. Various diseases against which research has been carried out are also reviewed. This article focused on the conception of edible vaccines highlighting the various ways by which vaccines can be delivered.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccines; Plants; Algae; Probiotics; Edible vaccine.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200301

Cholinergic stimulation with pyridostigmine reduces the QTc interval in coronary artery disease
Provedor de dados: 56
Autores: Castro,R.R.T.; Porphirio,G.; Serra,S.M.; Nóbrega,A.C.L.
Parasympathetic dysfunction is an independent risk factor in patients with coronary artery disease; thus, cholinergic stimulation is a potential therapeutic measure that may be protective by acting on ventricular repolarization. The purpose of the present study was to determine the effects of pyridostigmine bromide (PYR), a reversible anticholinesterase agent, on the electrocardiographic variables, particularly QTc interval, in patients with stable coronary artery disease. In a randomized double-blind crossover placebo-controlled study, simultaneous 12-lead electrocardiographic tracings were obtained at rest from 10 patients with exercise-induced myocardial ischemia before and 2 h after the oral administration of 45 mg PYR or placebo. PYR increased the RR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyridostigmine; QTc interval; Stable angina; Anticholinesterase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600008

Collagen XVIII/endostatin expression in experimental endotoxemic acute renal failure
Provedor de dados: 56
Autores: Cichy,M.C.; Rocha,F.G.G.; Tristão,V.R.; Pessoa,E.A.; Cenedeze,M.A.; Nürmberg Junior,R.; Schor,N.; Bellini,M.H.
Acute renal failure (ARF) is a frequent complication of Gram-negative sepsis, with a high risk of mortality. Lipopolysaccharide (LPS)-induced ARF is associated with hemodynamic changes that are strongly influenced by the overproduction of nitric oxide (NO) through the cytokine-mediated up-regulation of inducible NO synthase. LPS-induced reductions in systemic vascular resistance paradoxically culminate in renal vasoconstriction. Collagen XVIII is an important component of the extracellular matrix expressed in basement membranes. Its degradation by matrix metalloproteases, cathepsins and elastases results in the formation of endostatin, claimed to have antiangiogenic activity and to be a prominent vasorelaxing agent. We evaluated the expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute renal failure; Lipopolysaccharide; Gene expression; Endostatin; CD31.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200005

Time course of the hemodynamic responses to aortic depressor nerve stimulation in conscious spontaneously hypertensive rats
Provedor de dados: 56
Autores: Durand,M.T.; Mota,A.L.; Barale,A.R.; Castania,J.A.; Fazan Jr.,R.; Salgado,H.C.
The time to reach the maximum response of arterial pressure, heart rate and vascular resistance (hindquarter and mesenteric) was measured in conscious male spontaneously hypertensive (SHR) and normotensive control rats (NCR; Wistar; 18-22 weeks) subjected to electrical stimulation of the aortic depressor nerve (ADN) under thiopental anesthesia. The parameters of stimulation were 1 mA intensity and 2 ms pulse length applied for 5 s, using frequencies of 10, 30, and 90 Hz. The time to reach the hemodynamic responses at different frequencies of ADN stimulation was similar for SHR (N = 15) and NCR (N = 14); hypotension = NCR (4194 ± 336 to 3695 ± 463 ms) vs SHR (3475 ± 354 to 4494 ± 300 ms); bradycardia = NCR (1618 ± 152 to 1358 ± 185 ms) vs SHR (1911 ± 323 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrical stimulation; Arterial pressure; Aortic depressor nerve; Baroreflex; Spontaneously hypertensive rats; Vascular tone.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500010

Co-culture of apoptotic breast cancer cells with immature dendritic cells: a novel approach for DC-based vaccination in breast cancer
Provedor de dados: 56
Autores: Zheng,Jin; Liu,Qiang; Yang,Jiandong; Ren,Qinyou; Cao,Wei; Yang,Jingyue; Yu,Zhaocai; Yu,Fang; Wu,Yanlan; Shi,Hengjun; Liu,Wenchao.
A dendritic cell (DC)-based vaccine strategy could reduce the risk of recurrence and improve the survival of breast cancer patients. However, while therapy-induced apoptosis of hepatocellular and colorectal carcinoma cells can enhance maturation and antigen presentation of DCs, whether this effect occurs in breast cancer is currently unknown. In the present study, we investigated the effect of doxorubicin (ADM)-induced apoptotic MCF-7 breast cancer cells on the activation of DCs. ADM-induced apoptotic MCF-7 cells could effectively induce immature DC (iDC) maturation. The mean fluorescence intensity (MFI) of DC maturity marker CD83 was 23.3 in the ADM-induced apoptotic MCF-7 cell group compared with 8.5 in the MCF-7 cell group. The MFI of DC co-stimulatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Dendritic cells; Immunotherapy; Apoptosis; Doxorubicin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600006

Influence of exercise modality on agreement between gas exchange and heart rate variability thresholds
Provedor de dados: 56
Autores: Cunha,F.A.; Montenegro,R.A.; Midgley,A.W.; Vasconcellos,F.; Soares,P.P.; Farinatti,P.
The main purpose of this study was to investigate the level of agreement between the gas exchange threshold (GET) and heart rate variability threshold (HRVT) during maximal cardiopulmonary exercise testing (CPET) using three different exercise modalities. A further aim was to establish whether there was a 1:1 relationship between the percentage heart rate reserve (%HRR) and percentage oxygen uptake reserve (   %    V ˙ O 2 R) at intensities corresponding to GET and HRVT. Sixteen apparently healthy men 17 to 28 years of age performed three maximal CPETs (cycling, walking, and running). Mean heart rate and       V ˙ O 2 at GET and HRVT were 16 bpm (P&lt;0.001) and 5.2 mL·kg-1·min-1 (P=0.001) higher in running than cycling, but no significant differences were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aerobic exercise; Autonomic nervous system; Heart rate reserve; Oxygen uptake reserve; Poincare plot; Ramp protocol.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800706

A novel effective method for the assessment of microvascular function in male patients with coronary artery disease: a pilot study using laser speckle contrast imaging
Provedor de dados: 56
Autores: Borges,J.P.; Lopes,G.O.; Verri,V.; Coelho,M.P.; Nascimento,P.M.C.; Kopiler,D.A.; Tibirica,E.
Evaluation of microvascular endothelial function is essential for investigating the pathophysiology and treatment of cardiovascular and metabolic diseases. Although laser speckle contrast imaging technology is well accepted as a noninvasive methodology for assessing microvascular endothelial function, it has never been used to compare male patients with coronary artery disease with male age-matched healthy controls. Thus, the aim of this study was to determine whether laser speckle contrast imaging could be used to detect differences in the systemic microvascular functions of patients with established cardiovascular disease (n=61) and healthy age-matched subjects (n=24). Cutaneous blood flow was assessed in the skin of the forearm using laser speckle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemic heart disease; Cutaneous vascular conductance; Microvascular flowmetry; Endothelial function; Post-occlusive reactive hyperemia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000703

Neural regulation of the stress response: glucocorticoid feedback mechanisms
Provedor de dados: 56
Autores: Herman,J.P.; McKlveen,J.M.; Solomon,M.B.; Carvalho-Netto,E.; Myers,B.
The mammalian stress response is an integrated physiological and psychological reaction to real or perceived adversity. Glucocorticoids are an important component of this response, acting to redistribute energy resources to both optimize survival in the face of challenge and to restore homeostasis after the immediate challenge has subsided. Release of glucocorticoids is mediated by the hypothalamo-pituitary-adrenal (HPA) axis, driven by a neural signal originating in the paraventricular nucleus (PVN). Stress levels of glucocorticoids bind to glucocorticoid receptors in multiple body compartments, including the brain, and consequently have wide-reaching actions. For this reason, glucocorticoids serve a vital function in negative feedback inhibition of their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypothalamo-pituitary-adrenocortical axis; Corticotropin-releasing hormone; Glucocorticoid receptor; Amygdala; Hippocampus; Prefrontal cortex.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400002

Distribution of HLA-DRB1 alleles in a mixed population with insulin-dependent diabetes mellitus from the Southeast of Brazil
Provedor de dados: 56
Autores: Marques,S.B.D.; Volpini,W.; Caillat-Zucman,S.; Lieber,S.R.; Pavin,E.J.; Persoli,L.B.
HLA class II genes are strongly associated with susceptibility and resistance to insulin-dependent diabetes mellitus (IDDM). The present study reports the HLA-DRB1 genotyping of 41 IDDM patients and 99 healthy subjects from the Southeast of Brazil (Campinas region). Both groups consisted of an ethnic mixture of Caucasian, African Negro and Amerindian origin. HLA-DRB1*03 and *04 alleles were found at significantly higher frequencies among IDDM patients compared to the controls (DRB1*03: 48.8% vs 18.2%, P&amp;lt;0.005, RR = 4.27; DRB1*04: 43.9% vs 15.1%, P&amp;lt;0.008, RR = 4.37) and were associated with a susceptibility to the disease. DRB1*03/*04 heterozygosity conferred a strong IDDM risk (RR = 5.44). In contrast, the HLA-DRB1*11 allele frequency was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin-dependent diabetes mellitus; Genetic markers; DNA typing; HLA-DRB1.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300005

Role of microRNAs in the treatment of type 2 diabetes mellitus with Roux-en-Y gastric bypass
Provedor de dados: 56
Autores: Zhu,Z.; Yin,J.; Li,D.C.; Mao,Z.Q.
The aim of this study was to investigate the effect of Roux-en-Y gastric bypass (RYGB) on the peripheral blood microRNAs (miRNAs) of patients with type 2 diabetes mellitus (T2DM). miRNAs are small 20- to 22-nucleotide (nt) noncoding RNAs. They constitute a novel class of gene regulators that negatively regulate gene expression at the post-transcriptional level. miRNAs play an important role in several biological processes. Twelve patients with T2DM who were scheduled to undergo laparoscopic RYGB surgery were separated into two groups, using a body mass index of 30 kg/m2 as a cut-off point. Venous blood was collected before operation and 12 months after operation. A significant change was observed in the peripheral blood miRNA expression profile of both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expression profile; Methylation; MicroRNA; Roux-en-Y gastric bypass; Type 2 diabetes mellitus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300705

Effect of different anesthetic agents on left ventricular systolic function assessed by echocardiography in hamsters
Provedor de dados: 56
Autores: Tanaka,D.M.; Romano,M.M.D.; Carvalho,E.E.V.; Oliveira,L.F.L.; Souza,H.C.D.; Maciel,B.C.; Salgado,H.C.; Fazan-Júnior,R.; Simões,M.V.
Determination of left ventricular ejection fraction (LVEF) using in vivo imaging is the cardiac functional parameter most frequently employed in preclinical research. However, there is considerable conflict regarding the effects of anesthetic agents on LVEF. This study aimed at assessing the effects of various anesthetic agents on LVEF in hamsters using transthoracic echocardiography. Twelve female hamsters were submitted to echocardiography imaging separated by 1-week intervals under the following conditions: 1) conscious animals, 2) animals anesthetized with isoflurane (inhaled ISO, 3 L/min), 3) animals anesthetized with thiopental (TP, 50 mg/kg, intraperitoneal), and 4) animals anesthetized with 100 mg/kg ketamine plus 10 mg/kg xylazine injected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echocardiography; Anesthetics; Systolic function; Hamsters.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000602

YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy
Provedor de dados: 56
Autores: Jin,Bo; Zhu,Jun; Shi,Hai-Ming; Wen,Zhi-Chao; Wu,Bang-Wei.
Yes-associated protein (YAP) is an important regulator of cellular proliferation and transdifferentiation. However, little is known about the mechanisms underlying myofibroblast transdifferentiation in dilated cardiomyopathy (DCM). We investigated the role of YAP in the pathological process of cardiac matrix remodeling. A classic model of DCM was established in BALB/c mice by immunization with porcine cardiac myosin. Cardiac fibroblasts were isolated from neonatal Sprague-Dawley rats by density gradient centrifugation. The expression levels of α-smooth muscle actin (α-SMA) and collagen volume fraction (CVF) were significantly increased in DCM mice. Angiotensin II (Ang II)-mediated YAP activation promoted the proliferation and transdifferentiation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YAP; Fibroblast; Cardiac remodeling; Dilated cardiomyopathy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100605

Effect of salt intake and potassium supplementation on brachial-ankle pulse wave velocity in Chinese subjects: an interventional study
Provedor de dados: 56
Autores: Wang,Y.; Mu,J.J.; Geng,L.K.; Wang,D.; Ren,K.Y.; Guo,T.S.; Chu,C.; Xie,B.Q.; Liu,F.Q.; Yuan,Z.Y.
Accumulating evidence has suggested that high salt and potassium might be associated with vascular function. The aim of this study was to investigate the effect of salt intake and potassium supplementation on brachial-ankle pulse wave velocity (PWV) in Chinese subjects. Forty-nine subjects (28-65 years of age) were selected from a rural community of northern China. All subjects were sequentially maintained on a low-salt diet for 7 days (3.0 g/day NaCl), a high-salt diet for an additional 7 days (18.0 g/day NaCl), and a high-salt diet with potassium supplementation for a final 7 days (18.0 g/day NaCl+4.5 g/day KCl). Brachial-ankle PWV was measured at baseline and on the last day of each intervention. Blood pressure levels were significantly increased from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulse wave velocity; Salt; Potassium; Interventional study.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100083

Altered peripheral lymphocyte subsets in untreated systemic lupus erythematosus patients with infections
Provedor de dados: 56
Autores: Lu,Zhimin; Li,Jing; Ji,Juan; Gu,Zhifeng; Da,Zhanyun.
The leading cause of death in systemic lupus erythematosus (SLE) patients is infection. The objective of this study was to evaluate the distribution of lymphocyte subsets in untreated SLE patients with infections. This was a cross-sectional study. Data from January 2017 to May 2018 were collected. Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+CD56NK cells, and CD3+CD16+CD56NKT cells in 25 healthy controls and 52 treatment-naive SLE patients, among whom 13 were complicated with infections. Association between the lymphocyte subsets and infections was further analyzed. SLE patients with infections (n=13) showed a significantly higher incidence rate of fever (84.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Lymphocyte subsets; Infection; C-reactive protein; Procalcitonin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400609

Searching for the role of protein phosphatases in eukaryotic microorganisms
Provedor de dados: 56
Autores: da-Silva,A.M.; Zapella,P.D.A.; Andrioli,L.P.M.; Campanhã,R.B.; Fiorini,L.C.; Etchebehere,L.C.; da-Costa-Maia,J.C.; Terenzi,H.F.
Preference for specific protein substrates together with differential sensitivity to activators and inhibitors has allowed classification of serine/threonine protein phosphatases (PPs) into four major types designated types 1, 2A, 2B and 2C (PP1, PP2A, PP2B and PP2C, respectively). Comparison of sequences within their catalytic domains has indicated that PP1, PP2A and PP2B are members of the same gene family named PPP. On the other hand, the type 2C enzyme does not share sequence homology with the PPP members and thus represents another gene family, known as PPM. In this report we briefly summarize some of our studies about the role of serine/threonine phosphatases in growth and differentiation of three different eukaryotic models: Blastocladiella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein phosphatases; Blastocladiella emersonii; Neurospora crassa; Dictyostelium discoideum.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700006

An electronic pressure-meter nociception paw test for rats
Provedor de dados: 56
Autores: Vivancos,G.G.; Verri Jr.,W.A.; Cunha,T.M.; Schivo,I.R.S.; Parada,C.A.; Cunha,F.Q.; Ferreira,S.H.
The objective of the present investigation was to compare the sensitivity of an electronic nociceptive mechanical paw test with classical mechanical tests to quantify the intensity variation of inflammatory nociception. The electronic pressure-meter test consists of inducing the hindpaw flexion reflex by poking the plantar region with a polypropylene pipette tip adapted to a hand-held force transducer. This method was compared with the classical von Frey filaments test and with the rat paw constant pressure test, a modification of the Randall and Selitto test developed by our group. When comparing the three methods, the electronic pressure-meter and the rat paw constant pressure test, but not the von Frey filaments test, detected time vs treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperalgesia; Nociception; Dipyrone; Indomethacin; Von Frey filaments; Constant pressure test.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300017

Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
Provedor de dados: 56
Autores: Barros,M.A.V.; Andrade,E.B.; Barros,R.G.N.; Costa,I.K.M.; Costa,I.C.L.; Vitorino,G.F.A.; Andrade,J.J.C.; Paulino-Silva,K.M.; Nogueira,V.O.; de Brito Alves,J.L.; Costa-Silva,J.H.
The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P&gt;0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Development; Epigenetics; Fertility; Gestation; Intrauterine growth; Nutrition.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613

Effect of 5-HT1B receptor agonists injected into the prefrontal cortex on maternal aggression in rats
Provedor de dados: 56
Autores: Veiga,C.P.; Miczek,K.A.; Lucion,A.B.; Almeida,R.M.M. de.
Serotonin (5-HT1B) receptors play an essential role in the inhibition of aggressive behavior in rodents. CP-94,253, a 5-HT1B receptor agonist, can reduce aggression in male mice when administered directly into the ventro-orbitofrontal (VO) prefrontal cortex (PFC). The objective of the current study was to assess the effects of two selective 5-HT1B receptor agonists (CP-94,253 and CP-93,129), microinjected into the VO PFC, on maternal aggressive behavior after social instigation in rats. CP-94,253 (0.56 µg/0.2 µL, N = 8, and 1.0 µg/0.2 µL, N = 8) or CP-93,129 (1.0 µg/0.2 µL, N = 9) was microinjected into the VO PFC of Wistar rats on the 9th day postpartum and 15 min thereafter the aggressive behavior by the resident female against a male intruder was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prefrontal cortex; 5-HT receptors; Serotonin; Maternal behavior; Aggression.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600012

Potentiation of carbon tetrachloride hepatotoxicity by pentosan polysulfate in rats
Provedor de dados: 56
Autores: Zim,M.C.A.; Silveira,T.R.; Schwartsmann,G.; Cerski,T.; Motta,A.
Few data are available in the literature regarding the effect of pentosan polysulfate (PPS) on normal and fibrotic rat livers. In addition, the combination of PPS and carbon tetrachloride (CCl4) has not been studied so far. The objective of this study was to assess the effect of PPS on rat livers treated or not with CCl4 for the induction of liver fibrosis. The study consisted of four stages: 1) hepatic fibrosis induction with CCl4 (N = 36 rats); 2) evaluation of the effect of PPS on CCl4-induced hepatic fibrosis (N = 36 rats); 3) evaluation of the effect of higher doses of PPS in combination with CCl4 (N = 50 rats); 4) evaluation of the presence of an enzymatic inductor effect by PPS (N = 18 rats) using the sodium pentobarbital test which indirectly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cirrhosis; Fibrogenesis; Hepatic necrosis; Pentosan polysulfate; Carbon tetrachloride.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100012

The role of necroptosis in neurosurgical diseases
Provedor de dados: 56
Autores: Liu,T.; Bao,Y.H.; Wang,Y.; Jiang,J.Y.
Programmed necrosis or necroptosis is an alternative form of cell death that is executed through a caspase-independent pathway. Necroptosis has been implicated in many pathological conditions. Genetic or pharmacological inhibition of necroptotic signaling has been shown to confer neuroprotection after traumatic and ischemic brain injury. Therefore, the necroptotic pathway represents a potential target for neurological diseases that are managed by neurosurgeons. In this review, we summarize recent advances in the understanding of necroptotic signaling pathways and explore the role of necroptotic cell death in craniocerebral trauma, brain tumors, and cerebrovascular diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular mechanism; Pathogenesis; Programmed cell death; Therapy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400292

Repeated exposure of adolescent rats to oral methylphenidate does not induce behavioral sensitization or cross-sensitization to nicotine
Provedor de dados: 56
Autores: Justo,C.C.; Carneiro-de-Oliveira,P.E.; DeLucia,R.; Aizenstein,M.L.; Planeta,C.S.
Several lines of evidence indicate that the use of stimulant drugs, including methylphenidate (MPD), increases tobacco smoking. This has raised concerns that MPD use during adolescence could facilitate nicotine abuse. Preclinical studies have shown that repeated treatment with an addictive drug produces sensitization to that drug and usually cross-sensitization to other drugs. Behavioral sensitization has been implicated in the development of drug addiction. We examined whether repeated oral MPD administration during adolescence could induce behavioral sensitization to MPD and long-lasting cross-sensitization to nicotine. Adolescent male Wistar rats were treated orally with 10 mg/kg MPD or saline (SAL) from postnatal day (PND) 27 to 33. To evaluate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylphenidate; Nicotine; Behavioral sensitization; Adolescent rats.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700007

An analysis of the electrocardiogram QT interval
Provedor de dados: 56
Autores: Koehler,N.R.; Zouvi,J.P.; Acevedo,L.A.; Sukienik,B.J.; Rabin,M.
No reports testing the efficacy of the use of the QT/RR ratio &lt;1/2 for detecting a normal QTc interval were found in the literature. The objective of the present study was to determine if a QT/RR ratio &lt;=1/2 can be considered to be equal to the normal QTc and to compare the QT and QTc measured and calculated clinically and by a computerized electrocardiograph. Ratios (140 QT/RR) of 28 successive electrocardiograms obtained from 28 consecutive patients in a tertiary level teaching hospital were analyzed clinically by 5 independent observers and by a computerized electrocardiograph. The QT/RR ratio provided 56% sensitivity and 78% specificity, with an area under the receiver operator characteristic curve of 75.8% (95%CI: 0.68 to 0.84). The divergence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrocardiography; Long QT syndrome; Arrhythmia; QT interval.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100004

Molecular biology of the kallikrein-kinin system: from structure to function
Provedor de dados: 56
Autores: Pesquero,J.B.; Bader,M.
The participation of the kallikrein-kinin system, comprising the serine proteases kallikreins, the protein substrates kininogens and the effective peptides kinins, in some pathological processes like hypertension and cardiovascular diseases is still a matter of controversy. The use of different experimental set-ups in concert with the development of potent and specific inhibitors and antagonists for the system has highlighted its importance but the results still lack conclusivity. Over the last few years, transgenic and gene-targeting technologies associated with molecular biology tools have provided specific information about the elusive role of the kallikrein-kinin system in the control of blood pressure and electrolyte homeostasis. cDNA and genomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kallikrein; Kinins; Bradykinin; Molecular biology; Transgenics.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900013

The influence of stress on fear memory processes
Provedor de dados: 56
Autores: Martijena,I.D.; Molina,V.A.
It is well recognized that stressful experiences promote robust emotional memories, which are well remembered. The amygdaloid complex, principally the basolateral complex (BLA), plays a pivotal role in fear memory and in the modulation of stress-induced emotional responses. A large number of reports have revealed that GABAergic interneurons provide a powerful inhibitory control of the activity of projecting glutamatergic neurons in the BLA. Indeed, a reduced GABAergic control in the BLA is essential for the stress-induced influence on the emergence of associative fear memory and on the generation of long-term potentiation (LTP) in BLA neurons. The extracellular signal-regulated kinase (ERK) subfamily of the mitogen-activated protein kinase (MAPK) signaling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress; Amygdala; GABA; Memory; Fear.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400004

Molecular mimicry between cardiac myosin and Trypanosoma cruzi antigen B13: identification of a B13-driven human T cell clone that recognizes cardiac myosin
Provedor de dados: 56
Autores: Abel,L.C.J.; Kalil,J.; Cunha-Neto,E.
Previous reports from our group have demonstrated the association of molecular mimicry between cardiac myosin and the immunodominant Trypanosoma cruzi protein B13 with chronic Chagas' disease cardiomyopathy at both the antibody and heart-infiltrating T cell level. At the peripheral blood level, we observed no difference in primary proliferative responses to T. cruzi B13 protein between chronic Chagas' cardiopathy patients, asymptomatic chagasics and normal individuals. In the present study, we investigated whether T cells sensitized by T. cruzi B13 protein respond to cardiac myosin. T cell clones generated from a B13-stimulated T cell line obtained from peripheral blood of a B13-responsive normal donor were tested for proliferation against B13 protein and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular mimicry; Crossreactive T cells; Cardiac myosin; Trypanosoma cruzi; Chagas' disease.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100007

Interaction between substance P and gastrin-releasing peptide on thyrotropin secretion by rat pituitary in vitro
Provedor de dados: 56
Autores: Moura,E.G.; Santos,C.V.M.; Santos,R.M.M.; Pazos-Moura,C.C.
The effect of substance P (SP) on thyrotropin (TSH) secretion is controversial. In this study we evaluated the effect of SP on TSH secretion by hemipituitaries of 3-month-old Wistar rats in vitro and its interaction with gastrin-releasing peptide (GRP) at equimolar concentrations (1 µM and 10 µM). TSH release was measured under basal conditions and 30 min after incubation in the absence or presence of SP, GRP or both peptides. Pituitary TSH content was also measured in the pituitary homogenate after incubation. SP at both concentrations caused a significant (P&amp;lt;0.05) increase in TSH secretion compared with all other groups, which was approximately 60% (1 µM) and 85% (10 µM) higher than that of the control group (23.3 ± 3.0 ng/ml). GRP at the lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Substance P; Thyrotropin; Gastrin-releasing peptide.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900015

Attenuation and immunogenicity of recombinant yellow fever 17D-dengue type 2 virus for rhesus monkeys
Provedor de dados: 56
Autores: Galler,R.; Marchevsky,R.S.; Caride,E.; Almeida,L.F.C.; Yamamura,A.M.Y.; Jabor,A.V.; Motta,M.C.A.; Bonaldo,M.C.; Coutinho,E.S.F.; Freire,M.S.
A chimeric yellow fever (YF)-dengue serotype 2 (dengue 2) virus was constructed by replacing the premembrane and envelope genes of the YF 17D virus with those from dengue 2 virus strains of Southeast Asian genotype. The virus grew to high titers in Vero cells and, after passage 2, was used for immunogenicity and attenuation studies in rhesus monkeys. Subcutaneous immunization of naive rhesus monkeys with the 17D-D2 chimeric virus induced a neutralizing antibody response associated with the protection of 6 of 7 monkeys against viremia by wild-type dengue 2 virus. Neutralizing antibody titers to dengue 2 were significantly lower in YF-immune animals than in YF-naive monkeys and protection against challenge with wild-type dengue 2 virus was observed in only 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YF 17D virus recombinants; Dengue vaccine; Immunogenicity; Attenuation; Rhesus monkeys.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200012

Lack of evidence for monosomy 1p36 in patients with Prader-Willi-like phenotype
Provedor de dados: 56
Autores: Rodríguez,V.R.; Mazzucato,L.F.; Pina-Neto,J.M.
Monosomy 1p36 is the most common subtelomeric microdeletion syndrome with an incidence rate estimated to be 1 in 5000 births. A hypothesis of a similarity between patients with 1p36 deletion and those with Prader-Willi syndrome and the existence of two different phenotypes for 1p36 microdeletion has been suggested. The main objective of the present study was to determine the existence of 1p36 microdeletion in a sample of patients with mental retardation, obesity and hyperphagia who tested negative by the methylation test for Prader-Willi syndrome. Sixteen patients (7 females, 9 males), 16-26 years old, were evaluated with high-resolution cytogenetic analysis at 550-850 band levels and with 11 polymorphic microsatellite markers located in the 1p36 region....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Monosomy 1p36; 1p36 microdeletion syndrome; Prader-Willi syndrome; Prader-Willi-like phenotype.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800007

Effect of practice and span length on the dual-task coordination executive test
Provedor de dados: 56
Autores: Sylwan,R.P.; Rosin,F.M.; Galera,C.
The measure "mu", proposed as an index of the ability to coordinate concurrent box-crossing (BC) and digit-span (DS) tasks in the dual task (DT), should reflect the capacity of the executive component of the working memory system. We investigated the effect of practice in BC and of a change in the digit span on mu by adding previous practice trials in BC and diminishing, maintaining or increasing the digit sequence length. The mu behavior was evaluated throughout three trials of the test. Reported strategies in digit tasks were also analyzed. Subjects with diminished span showed the best performance in DT due to a stable performance in DS and BC in the single- and dual-task conditions. These subjects also showed a more stable performance throughout trials....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dual task; Working memory; Central executive; Digit span; Practice.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000013

Application of data mining techniques to explore predictors of upper urinary tract damage in patients with neurogenic bladder
Provedor de dados: 56
Autores: Fang,H.; Lu,B.; Wang,X.; Zheng,L.; Sun,K.; Cai,W.
This study proposed a decision tree model to screen upper urinary tract damage (UUTD) for patients with neurogenic bladder (NGB). Thirty-four NGB patients with UUTD were recruited in the case group, while 78 without UUTD were included in the control group. A decision tree method, classification and regression tree (CART), was then applied to develop the model in which UUTD was used as a dependent variable and history of urinary tract infections, bladder management, conservative treatment, and urodynamic findings were used as independent variables. The urethra function factor was found to be the primary screening information of patients and treated as the root node of the tree; Pabd max (maximum abdominal pressure, &gt;14 cmH2O), Pves max (maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurogenic bladder; Upper urinary tract damage; Decision tree model; Urodynamics; Urethra function.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000603

Changes in sodium appetite evoked by lesions of the commissural nucleus of the tractus solitarius
Provedor de dados: 56
Autores: Ogihara,C.A.; Schoorlemmer,G.H.M.; Colombari,E.; Sato,M.A.
Ablation of the area postrema/caudal nucleus of the tractus solitarius (NTS) complex increases sodium intake, but the effect of selective lesions of the caudal NTS is not known. We measured depletion-induced sodium intake in rats with electrolytic lesions of the commissural NTS that spared the area postrema. One day after the lesion, rats were depleted of sodium with furosemide (10 mg/kg body weight, sc) and then had access to water and a sodium-deficient diet for 24 h when 1.8% NaCl was offered. Water and saline intakes were measured for 2 h. Saline intake was higher in lesioned than in sham-lesioned rats (mean ± SEM: 20 ± 2 vs 11 ± 3 mL/2 h, P &lt; 0.05, N = 6-7). Saline intake remained elevated in lesioned rats when the tests were repeated 6 and 14 days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleus of the solitary tract; Sodium appetite; Sucrose; Furosemide.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600014

Effect of trimetazidine treatment on the transient outward potassium current of the left ventricular myocytes of rats with streptozotocin-induced type 1 diabetes mellitus
Provedor de dados: 56
Autores: Xiang,Yu-luan; He,Li; Xiao,Jun; Xia,Shuang; Deng,Song-bai; Xiu,Yun; She,Qiang.
Cardiovascular complications are a leading cause of mortality in patients with diabetes mellitus (DM). The present study was designed to investigate the effects of trimetazidine (TMZ), an anti-angina drug, on transient outward potassium current (Ito) remodeling in ventricular myocytes and the plasma contents of free fatty acid (FFA) and glucose in DM. Sprague-Dawley rats, 8 weeks old and weighing 200-250 g, were randomly divided into three groups of 20 animals each. The control group was injected with vehicle (1 mM citrate buffer), the DM group was injected with 65 mg/kg streptozotocin (STZ) for induction of type 1 DM, and the DM + TMZ group was injected with the same dose of STZ followed by a 4-week treatment with TMZ (60 mg·kg-1·day-1). All animals were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transient outward K+ current; Free fatty acid; Trimetazidine; Diabetic cardiomyopathy; Channel protein; Ventricular myocytes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300004

Optimized Fast-FISH with a-satellite probes: acceleration by microwave activation
Provedor de dados: 56
It has been shown for several DNA probes that the recently introduced Fast-FISH (fluorescence in situ hybridization) technique is well suited for quantitative microscopy. For highly repetitive DNA probes the hybridization (renaturation) time and the number of subsequent washing steps were reduced considerably by omitting denaturing chemical agents (e.g., formamide). The appropriate hybridization temperature and time allow a clear discrimination between major and minor binding sites by quantitative fluorescence microscopy. The well-defined physical conditions for hybridization permit automatization of the procedure, e.g., by a programmable thermal cycler. Here, we present optimized conditions for a commercially available X-specific &lt;FONT...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fluorescence in situ hybridization Fast-FISH metaphase chromosomes &lt; FONT FACE=Symbol&gt; A&lt; /font&gt; -satellite DNA probes microwave treatment quantitative image analysis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100003

Mineralocorticoid modulation of central angiotensin-induced neuronal activity, water intake and sodium appetite
Provedor de dados: 56
Autores: Thornton,S.N.; Omouessi,S.T.; Falconetti,C.
Central angiotensin II (AngII) stimulates water and salt solution intake. Pretreatment with low-dose mineralocorticoid (DOCA) enhances this AngII-induced intake of salt solutions (the synergy theory) in Wistar and Sprague Dawley rats but not in Fischer rats. This response is mediated via the AT-1 receptor. Electrophysiological experiments using iontophoretic application of AngII and the AT-1 receptor-specific non-peptide antagonist losartan showed excitation of neurons in the preoptic/medial septum region of urethane-anesthetized male Wistar rats. DOCA pretreatment further enhances this neuronal excitation in response to AngII and reduces the responses to losartan. This generated the hypothesis that DOCA-enhanced AngII-induced neuronal excitation is the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aldosterone; Rat; AT-1; AT-2; Iontophoresis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500014

Macelignan inhibits bee pathogenic fungi Ascophaera apis growth through HOG1 pathway
Provedor de dados: 56
Autores: Shin,Y.K.; Kim,K.Y.
Ascosphaera apis is a bee pathogen that causes bee larvae infection disease, to which treatment is not yet well investigated. The aim of this study was to investigate antifungal susceptibility in vitro against A. apis and to identify a new antifungal agent for this pathogen through minimal inhibitory concentration (MIC) assay and western blot analysis. Macelignan had 1.56 and 3.125 μg/mL MIC against A. apis after 24 and 48 h, respectively, exhibiting the strongest growth inhibition against A. apis among the tested compounds (corosolic acid, dehydrocostus lactone, loganic acid, tracheloside, fangchinoline and emodin-8-O-β-D-glucopyranoside). Furthermore, macelignan showed a narrow-ranged spectrum against various fungal strains without any mammalian cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bee; Ascosphaera apis; Macelignan; Antifungal agent; HOG1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700607

Imbalance of naive and memory T lymphocytes with sustained high cellular activation during the first year of life from uninfected children born to HIV-1-infected mothers on HAART
Provedor de dados: 56
Autores: Ono,E.; Santos,A.M. Nunes dos; Succi,R.C. de Menezes; Machado,D.M.; Angelis,D.S.A. de; Salomão,R.; Kallás,E.G.; Moraes-Pinto,M.I. de.
The immune consequences of in utero HIV exposure to uninfected children whose mothers were submitted to highly active antiretroviral therapy (HAART) during gestation are not well defined. We evaluated 45 HIV-exposed uninfected (ENI) neonates and 45 healthy unexposed control (CT) neonates. All HIV-infected mothers received HAART during pregnancy, and the viral load at delivery was &lt;50 copies/mL for 56.8%. Twenty-three ENI neonates were further evaluated after 12 months and compared to 23 unexposed healthy age-matched infants. Immunophenotyping was performed by flow cytometry in cord and peripheral blood. Cord blood lymphocyte numbers did not differ between groups. However, ENI neonates had a lower percentage of naive T cells than CT neonates (CD4+, 76.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunophenotyping; Highly active antiretroviral therapy; HIV-1; Immunological stimulation; Neonates of HIV-infected mothers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800011

Hepatic damage during acute pancreatitis in the rat
Provedor de dados: 56
Autores: Coelho,A.M.M.; Machado,M.C.C.; Sampietre,S.N.; Leite,K.R.M.; Oliveira,V.L.L.; Pinotti,H.W.
We studied the alterations in the metabolism of liver mitochondria in rats with acute pancreatitis. Male Wistar rats were allocated to a control group (group I) and to five other groups corresponding to 2, 4, 12, 24 and 48 h after the induction of acute pancreatitis by the injection of 5% sodium taurocholate into the pancreatic duct. Sham-operated animals were submitted to the same surgical steps except for the induction of acute pancreatitis. Mitochondrial oxidation and phosphorylation were measured polarographically by determining oxygen consumption without ADP (basal respiration, state 4) and in the presence of ADP (activated respiration, state 3). Serum amylase, transaminases (ALT and AST) and protein were also determined. Ascitic fluid, contents of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute pancreatitis; Hepatic lesion; Mitochondria.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800006

Development of new heparin-like compounds and other antithrombotic drugs and their interaction with vascular endothelial cells
Provedor de dados: 56
Autores: Nader,H.B.; Pinhal,M.A.S.; Baú,E.C.; Castro,R.A.B.; Medeiros,G.F.; Chavante,S.F.; Leite,E.L.; Trindade,E.S.; Shinjo,S.K.; Rocha,H.A.O.; Tersariol,I.L.S.; Mendes,A.; Dietrich,C.P.
The anticlotting and antithrombotic activities of heparin, heparan sulfate, low molecular weight heparins, heparin and heparin-like compounds from various sources used in clinical practice or under development are briefly reviewed. Heparin isolated from shrimp mimics the pharmacological activities of low molecular weight heparins. A heparan sulfate from Artemia franciscana and a dermatan sulfate from tuna fish show a potent heparin cofactor II activity. A heparan sulfate derived from bovine pancreas has a potent antithrombotic activity in an arterial and venous thrombosis model with a negligible activity upon the serine proteases of the coagulation cascade. It is suggested that the antithrombotic activity of heparin and other antithrombotic agents is due...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparin; Anticlotting activity heparan sulfate; Antithrombotic activity low molecular weight heparins; Preparation and antithrombotic activity antithrombotic agents; Development for clinical use crustacean heparin-like compounds; Antithrombotic activity antithrombotic agents vascular endothelial cells.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600002

Leptospira interrogans activation of peripheral blood monocyte glycolipoprotein demonstrated in whole blood by the release of IL-6
Provedor de dados: 56
Autores: Dorigatti,F.; Brunialti,M.K.C.; Romero,E.C.; Kallas,E.G.; Salomão,R.
Glycolipoprotein (GLP) from pathogenic serovars of Leptospira has been implicated in the pathogenesis of leptospirosis by its presence in tissues of experimental animals with leptospirosis, the inhibition of the Na,K-ATPase pump activity, and induced production of cytokines. The aims of the present study were to investigate the induction of IL-6 by GLP in peripheral blood mononuclear cells (PBMC) and to demonstrate monocyte stimulation at the cellular level in whole blood from healthy volunteers. PBMC were stimulated with increasing concentrations (5 to 2500 ng/ml) of GLP extracted from the pathogenic L. interrogans serovar Copenhageni, lipopolysaccharide (positive control) or medium (negative control), and supernatants were collected after 6, 20/24, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glycolipoprotein; Leptospira interrogans; Interleukin-6; Monocytes; CD45; CD14.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600013

Fuzzy expert system in the prediction of neonatal resuscitation
Provedor de dados: 56
Autores: Reis,M.A.M.; Ortega,N.R.S.; Silveira,P.S.P.
In view of the importance of anticipating the occurrence of critical situations in medicine, we propose the use of a fuzzy expert system to predict the need for advanced neonatal resuscitation efforts in the delivery room. This system relates the maternal medical, obstetric and neonatal characteristics to the clinical conditions of the newborn, providing a risk measurement of need of advanced neonatal resuscitation measures. It is structured as a fuzzy composition developed on the basis of the subjective perception of danger of nine neonatologists facing 61 antenatal and intrapartum clinical situations which provide a degree of association with the risk of occurrence of perinatal asphyxia. The resulting relational matrix describes the association between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fuzzy sets; Risk analysis; Medical expert systems; Perinatal asphyxia; Neonatal resuscitation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500018

Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo
Provedor de dados: 56
Specific glycosphingolipid antigens of Leishmania (L.) amazonensis amastigotes reactive with the monoclonal antibodies (MoAbs) ST-3, ST-4 and ST-5 were isolated, and their structure was partially elucidated by negative ion fast atom bombardment mass spectrometry. The glycan moieties of five antigens presented linear sequences of hexoses and N-acetylhexosamines ranging from four to six sugar residues, and the ceramide moieties were found to be composed by a sphingosine d18:1 and fatty acids 24:1 or 16:0. Affinities of the three monoclonal antibodies to amastigote glycosphingolipid antigens were also analyzed by ELISA. MoAb ST-3 reacted equally well with all glycosphingolipid antigens tested, whereas ST-4 and ST-5 presented higher affinities to...
Palavras-chave: Glycosphingolipids; Monoclonal antibodies; Leishmania (L.) amazonensis; Fast atom bombardment mass spectrometry; Carbohydrate antigens.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300014

The diversity of abnormal hormone receptors in adrenal Cushing's syndrome allows novel pharmacological therapies
Provedor de dados: 56
Autores: Lacroix,A.; N'Diaye,N.; Mircescu,H.; Tremblay,J.; Hamet,P.
Recent studies from several groups have indicated that abnormal or ectopic expression and function of adrenal receptors for various hormones may regulate cortisol production in ACTH-independent hypercortisolism. Gastric inhibitory polypeptide (GIP)-dependent Cushing's syndrome has been described in patients with either unilateral adenoma or bilateral macronodular adrenal hyperplasia; this syndrome results from the large adrenal overexpression of the GIP receptor without any activating mutation. We have conducted a systematic in vivo evaluation of patients with adrenal Cushing's syndrome in order to identify the presence of abnormal hormone receptors. In macronodular adrenal hyperplasia, we have identified, in addition to GIP-dependent Cushing's syndrome,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenal cortex; Cortisol; Cushing's syndrome; Ectopic expression; Hormone receptors.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000010

Reaction of diphenyl diselenide with hydrogen peroxide and inhibition of delta-aminolevulinate dehydratase from rat liver and cucumber leaves
Provedor de dados: 56
Autores: Farina,M.; Barbosa,N.B.V.; Nogueira,C.W.; Folmer,V.; Zeni,G.; Andrade,L.H.; Braga,A. L.; Rocha,J.B.T.
The interaction of the product of H2O2 and (PhSe)2 with delta-aminolevulinate dehydratase (delta-ALA-D) from mammals and plants was investigated. (PhSe)2 inhibited rat hepatic delta-ALA-D with an IC50 of 10 µM but not the enzyme from cucumber leaves. The reaction of (PhSe)2 with H2O2 for 1 h increased the inhibitory potency of the original compound and the IC50 for animal delta-ALA-D inhibition was decreased from 10 to 2 µM. delta-ALA-D from cucumber leaves was also inhibited by the products of reaction of (PhSe)2 with H2O2 with an IC50 of 4 µM. The major product of reaction of (PhSe)2 with H2O2 was identified as seleninic acid and produced an intermediate with a lambdamax at 265 nm after reaction with t-BuSH. These results suggest that the interaction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Delta-Aminolevulinate dehydratase; Diphenyl diselenide; Seleninic acid.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600001

Correlation of discocyte frequency and ATP concentration in preserved blood: A morphological indicator of red blood cell viability
Provedor de dados: 56
Red blood cells (RBC) are viable if kept in an adequate preservative solution, although gradual changes in morphology and metabolism may occur. There is a gradual decrease in adenosine-5'-triphosphate (ATP) concentration, pH, glucose consumption, and enzyme activity during preservation. The normal discocyte shapes are initially replaced by echinocytes and stomatocytes and, at final stages, by spherocytes, the last step before splenic sequestration. Post-transfusional survival has been correlated with the ATP concentration. RBC preserved in ADSOL, a solution containing adenine, dextrose, sodium chloride, and mannitol, are viable for transfusion for up to 6 weeks. Erythrocytes from 10 blood units taken from healthy adult donors were preserved for 12 weeks in...
Palavras-chave: Red blood cells; Adenosine-5'-triphosphate; Blood transfusion.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600007

Differences in synthesis and absorption of cholesterol of two effective lipid-lowering therapies
Provedor de dados: 56
Autores: Kasmas,S.H.; Izar,M.C.; França,C.N.; Ramos,S.C.; Moreira,F.T.; Helfenstein,T.; Moreno,R.A.; Borges,N.C.; Figueiredo-Neto,A.M.; Fonseca,F.A.
Effective statin therapy is associated with a marked reduction of cardiovascular events. However, the explanation for full benefits obtained for LDL cholesterol targets by combined lipid-lowering therapy is controversial. Our study compared the effects of two equally effective lipid-lowering strategies on markers of cholesterol synthesis and absorption. A prospective, open label, randomized, parallel design study, with blinded endpoints, included 116 subjects. We compared the effects of a 12-week treatment with 40 mg rosuvastatin or the combination of 40 mg simvastatin/10 mg ezetimibe on markers of cholesterol absorption (campesterol and β-sitosterol), synthesis (desmosterol), and their ratios to cholesterol. Both therapies similarly decreased total and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campesterol; Β-sitosterol; Desmosterol; Statin; Ezetimibe.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100015

Studies of the small bowel surface by scanning electron microscopy in infants with persistent diarrhea
Provedor de dados: 56
Autores: Fagundes-Neto,U.; De Martini-Costa,S.; Pedroso,M.Z.; Scaletsky,I.C.A.
We describe the ultrastructural abnormalities of the small bowel surface in 16 infants with persistent diarrhea. The age range of the patients was 2 to 10 months, mean 4.8 months. All patients had diarrhea lasting 14 or more days. Bacterial overgrowth of the colonic microflora in the jejunal secretion, at concentrations above 10(4) colonies/ml, was present in 11 (68.7%) patients. The stool culture was positive for an enteropathogenic agent in 8 (50.0%) patients: for EPEC O111 in 2, EPEC O119 in 1, EAEC in 1, and Shigella flexneri in 1; mixed infections due to EPEC O111 and EAEC in 1 patient, EPEC O119 and EAEC in 1 and EPEC O55, EPEC O111, EAEC and Shigella sonnei in 1. Morphological abnormalities in the small bowel mucosa were observed in all 16 patients,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Persistent diarrhea; Small bowel; Scanning electron microscopy; Bacterial proliferation; Infants.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200006

The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species
Provedor de dados: 56
Autores: Gomes,A.J.; Lunardi,C.N.; Gonzalez,S.; Tedesco,A.C.
Two natural products Polypodium leucotomos extract (PL) and kojic acid (KA) were tested for their ability to scavenge reactive oxygen species (·OH, ·O2-, H2O2, ¹O2) in phosphate buffer. Hydroxyl radicals were generated by the Fenton reaction, and the rate constants of scavenging were 1.6 x 10(9) M-1 s-1 for KA and 1.0 x 10(9) M-1 s-1 for PL, similar to that of ethanol (1.4 x 10(9) M-1 s-1). With superoxide anions generated by the xanthine/hypoxanthine system, KA and PL (0.2-1.0 mg/ml) inhibited ·O2-dependent reduction of nitroblue tetrazolium by up to 30 and 31%, respectively. In the detection of ¹O2 by rose bengal irradiation, PL at 1.0 mg/ml quenched singlet oxygen by 43% relative to azide and KA by 36%. The present study demonstrates that PL showed an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polypodium leucotomos extract; Kojic acid; Antioxidant; Reactive oxygen species.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100018

Reproducibility of carotid ultrasound measurements in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) at baseline
Provedor de dados: 56
Autores: Santos-Neto,P.J.; Sena-Santos,E.H.; Meireles,D.P.; Santos,I.S.; Bensenor,I.M.; Lotufo,P.A.
Carotid artery assessment by ultrasound is a non-invasive evaluation of subclinical atherosclerosis and a predictor of cardiovascular events. However, ultrasound examinations are operator-dependent. In the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil), ultrasound images have been acquired from more than 10,000 participants. In this article, we describe the reproducibility of carotid intima-media thickness (CIMT), carotid plaque detection, and carotid plaque score (defined as the number of arterial sites with plaques) using ELSA-Brasil protocol, in a subset of 118 participants. Two board-certified radiologists and a trained technician read carotid images. We calculated intra- and inter-observer intraclass correlation (ICC) for CIMT values. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plaque; Intima-media; Epidemiology; Ultrasound; Imaging.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800610

Bone mineral density and inflammatory bowel disease severity
Provedor de dados: 56
Autores: Lima,C.A.; Lyra,A.C.; Mendes,C.M.C.; Lopes,M.B.; Coqueiro,F.G.; Rocha,R.; Santana,G.O.
Inflammatory bowel disease (IBD) is associated with low bone mineral density (BMD). In this study, the association between disease severity and BMD in patients with IBD was evaluated. Associations between BMD and the Montreal classification, disease activity and drug therapy were also tested. A cross-sectional prevalence study with a comparison group was conducted. One hundred and twenty-eight patients were evaluated: 68 patients with ulcerative colitis (UC), and 60 with Crohn's disease (CD). The control group consisted of 67 healthy subjects. All patients and controls had BMD measured and in IBD patients, current medications, hospitalization, and disease location, extent and phenotype, according to the Montreal classification, were recorded. Multiple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone mineral density; Ulcerative colitis; Crohn’s disease; Inflammatory bowel disease; Osteoporosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200614

Effect of image resolution manipulation in rearfoot angle measurements obtained with photogrammetry
Provedor de dados: 56
Autores: Sacco,I.C.N.; Picon,A.P.; Ribeiro,A.P.; Sartor,C.D.; Camargo-Junior,F.; Macedo,D.O.; Mori,E.T.T.; Monte,F.; Yamate,G.Y.; Neves,J.G.; Kondo,V.E.; Aliberti,S.
The aim of this study was to investigate the influence of image resolution manipulation on the photogrammetric measurement of the rearfoot static angle. The study design was that of a reliability study. We evaluated 19 healthy young adults (11 females and 8 males). The photographs were taken at 1536 pixels in the greatest dimension, resized into four different resolutions (1200, 768, 600, 384 pixels) and analyzed by three equally trained examiners on a 96-pixels per inch (ppi) screen. An experienced physiotherapist marked the anatomic landmarks of rearfoot static angles on two occasions within a 1-week interval. Three different examiners had marked angles on digital pictures. The systematic error and the smallest detectable difference were calculated from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical therapy modalities; Photogrammetry; Reliability; Rearfoot angle.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900003

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil
Provedor de dados: 56
Autores: Rocha,G.A.; Oliveira,A.M.R.; Queiroz,D.M.M.; Mendes,E.N.; Moura,S.B.; Oliveira,C.A.; Ferrari,T.C.A.
We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from a developing country in view of variations in sensitivity and specificity reported for different populations. We studied 97 non-consecutive patients who underwent endoscopy for evaluation of dispeptic symptoms. The presence of H. pylori was determined in antral biopsy specimens by culture, by the preformed urease test and in carbolfuchsin-stained smears. Patients were considered to be H. pylori positive if at least two of the three tests presented a positive result or if the culture was positive, and negative if the three tests were negative. Sixty-five adults (31 with peptic ulcer) were H. pylori positive and 32 adults were H. pylori negative....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Serodiagnosis; ELISA.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000005

Antibacterial activity of indole alkaloids from Aspidosperma ramiflorum
Provedor de dados: 56
Autores: Tanaka,J.C.A.; Silva,C.C. da; Oliveira,A.J.B. de; Nakamura,C.V.; Dias Filho,B.P.
We evaluated the antibacterial activities of the crude methanol extract, fractions (I-V) obtained after acid-base extraction and pure compounds from the stem bark of Aspidosperma ramiflorum. The minimum inhibitory concentration (MIC) was determined by the microdilution technique in Mueller-Hinton broth. Inoculates were prepared in this medium from 24-h broth cultures of bacteria (10(7) CFU/mL). Microtiter plates were incubated at 37ºC and the MICs were recorded after 24 h of incubation. Two susceptibility endpoints were recorded for each isolate. The crude methanol extract presented moderate activity against the Gram-positive bacteria B. subtilis (MIC = 250 µg/mL) and S. aureus (MIC = 500 µg/mL), and was inactive against the Gram-negative bacteria E. coli...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aspidosperma ramiflorum; Antibacterial activity; Monoterpenoid indole alkaloids; Ramiflorines A and B.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300009

Identification of a new human mtDNA polymorphism (A14290G) in the NADH dehydrogenase subunit 6 gene
Provedor de dados: 56
Autores: Houshmand,M.; Mahmoudi,T.; Shafa Shariat Panahi,M.; Seyedena,Y.; Saber,S.; Ataei,M.
Leber's hereditary optic neuropathy (LHON) is a maternally inherited form of retinal ganglion cell degeneration leading to optic atrophy in young adults. Several mutations in different genes can cause LHON (heterogeneity). The ND6 gene is one of the mitochondrial genes that encodes subunit 6 of complex I of the respiratory chain. This gene is a hot spot gene. Fourteen Persian LHON patients were analyzed with single-strand conformational polymorphism and DNA sequencing techniques. None of these patients had four primary mutations, G3460A, G11788A, T14484C, and G14459A, related to this disease. We identified twelve nucleotide substitutions, G13702C, T13879C, T14110C, C14167T, G14199T, A14233G, G14272C, A14290G, G14365C, G14368C, T14766C, and T14798C. Eleven...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leber's hereditary optic neuropathy; ND6 gene; Mitochondrial DNA mutations; Single-strand conformational polymorphism; MtDNA A14290G allele.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600004

Contrast sensitivity to angular frequency stimuli is higher than that for sinewave gratings in the respective middle range
Provedor de dados: 56
This study compares contrast thresholds for sinewave gratings, or spatial frequencies (1/CSF) with contrast thresholds for angular frequencies (1/aCSF) and for radial frequencies, or J0 targets (1/rCSF). Observers had to differentiate between one of these frequency stimuli and a stimulus at mean luminance within a forced-choice procedure. All measurements were made with the same equipment, methods and subjects. Our results show higher sensitivity to, or lower thresholds for, angular frequencies when compared to either sinewave gratings or J0 targets. Contrast values in arbitrary units, in the lower threshold range for angular frequencies, were about half those required to differentiate sinewave gratings from mean luminance in its most sensitive range
Palavras-chave: Spatial frequency; CSF; J0 targets; Angular frequency; Contrast sensitivity; Contrast thresholds.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500010

Effects of propofol on damage of rat intestinal epithelial cells induced by heat stress and lipopolysaccharides
Provedor de dados: 56
Autores: Tang,J.; Jiang,Y.; Tang,Y.; Chen,B.; Sun,X.; Su,L.; Liu,Z.
Gut-derived endotoxin and pathogenic bacteria have been proposed as important causative factors of morbidity and death during heat stroke. However, it is still unclear what kind of damage is induced by heat stress. In this study, the rat intestinal epithelial cell line (IEC-6) was treated with heat stress or a combination of heat stress and lipopolysaccharide (LPS). In addition, propofol, which plays an important role in anti-inflammation and organ protection, was applied to study its effects on cellular viability and apoptosis. Heat stress, LPS, or heat stress combined with LPS stimulation can all cause intestinal epithelial cell damage, including early apoptosis and subsequent necrosis. However, propofol can alleviate injuries caused by heat stress, LPS,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; Heat stress; Lipopolysaccharides; Apoptosis; Intestinal epithelial cells.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600507

Random amplification of polymorphic DNA reveals clonal relationships among enteropathogenic Escherichia coli isolated from non-human primates and humans
Provedor de dados: 56
Autores: Carvalho,V.M.; Irino,K.; Onuma,D.; Castro,A.F. Pestana de.
Enteropathogenic Escherichia coli (EPEC) strains are important agents of infantile diarrhea all over the world, gaining even greater importance in developing countries. EPEC have also been isolated from various animal species, but most isolates belong to serotypes that differ from those recovered from humans. However, it has been demonstrated that several isolates from non-human primates belong to the serogroups and/or serotypes related to those implicated in human disease. The objective of this study was to evaluate the genetic differences between thirteen strains isolated from non-human primates and the same number of strains isolated from human infections. Human isolates belonged to the same serogroup/serotype as the monkey strains and the evaluation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Enteropathogenic Escherichia coli; Attaching and effacing Escherichia coli; Attaching and effacing; Monkey; Random amplified polymorphic DNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200010

Use of single photon emission computed tomography and magnetic resonance to evaluate central nervous system involvement in patients with juvenile systemic lupus erythematosus
Provedor de dados: 56
Autores: Prismich,G.; Hilário,M.O.E.; Len,C.A.; Terreri,M.T.; Quaresma,M.R.; Alonso,G.; Sevillano,M.M.; Lederman,H.M.
The objective of the present study was to identify the single photon emission computed tomography (SPECT) and magnetic resonance (MR) findings in juvenile systemic lupus erythematosus (JSLE) patients with CNS involvement and to try to correlate them with neurological clinical history data and neurological clinical examination. Nineteen patients with JSLE (16 girls and 3 boys, mean age at onset 9.2 years) were submitted to neurological examination, electroencephalography, cerebrospinal fluid analysis, SPECT and MR. All the evaluations were made separately within a period of 15 days. SPECT and MR findings were analyzed independently by two radiologists. Electroencephalography and cerebrospinal fluid analysis revealed no relevant alterations. Ten of 19...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Central nervous system; Single photon emission computed tomography; Magnetic resonance; Neurological clinical history.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700007

Hippocampal histamine receptors and conflictive exploration in the rat: studies using the elevated asymmetric plus-maze
Provedor de dados: 56
Autores: Ruarte,M.B.; Orofino,A.G.; Alvarez,E.O.
The possible role of histamine receptors in the hippocampal formation on the exploratory motivation and emotionality of the rat was studied. An elevated asymmetric plus-maze composed of 4 different arms (no walls, single high wall, high and low walls and two high walls) arranged at 90o angles was used. The exploration score, considered to be an index of exploratory motivation, and the permanency score, considered to be an index of emotionality (anxiety), were determined. Histamine was administered locally into the ventral hippocampus at three different doses (9, 45 and 90 nmol). Another group of rats was also microinjected with 45 nmol of pyrilamine (a histamine H1 receptor antagonist) or ranitidine (a histamine H2 receptor antagonist) in addition to 9...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emotionality; Motivation; Hippocampus; Histamine; Elevated asymmetric plus-maze.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200012

New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin
Provedor de dados: 56
Autores: Alves,A.M.B.; Lásaro,M.O.; Almeida,D.F.; Ferreira,L.C.S.
Stimulation of the mammalian immune system by administration of plasmid DNA has been shown to be an important approach for vaccine development against several pathogens. In the present study we investigated the use of DNA vaccines to induce immune responses against an enteric bacterial pathogen, enterotoxigenic Escherichia coli (ETEC). Three plasmid vectors encoding colonization factor antigen I (CFA/I), an ETEC fimbrial adhesin, were constructed. Eukaryotic cells transfected with each of these plasmids expressed the heterologous antigen in different compartments: bound to the cytoplasmic membrane (pRECFA), accumulated in the cytoplasm (pPolyCFA) or secreted to the outside medium (pBLCFA). BALB/c mice were intramuscularly (im) inoculated with purified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccines; ETEC; Diarrheal disease; CFA/I fimbriae.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200011

Flow rate, pH and calcium concentration of saliva of children and adolescents with type 1 diabetes mellitus
Provedor de dados: 56
Autores: Moreira,A.R.; Passos,I.A.; Sampaio,F.C.; Soares,M.S.M.; Oliveira,R.J.
Alterations in salivary parameters may increase the caries risk in diabetic children, but, contradictory data on this issue have been reported. The aims of this study were to compare salivary parameters (flow rate, pH and calcium concentration) between healthy and type 1 diabetes mellitus (T1DM) individuals. The sample consisted of 7- to 18-year-old individuals divided into two groups: 30 subjects with T1DM (group A) and 30 healthy control subjects (group B). Fasting glucose levels were determined. Unstimulated and stimulated saliva was collected. The pH of unstimulated saliva was measured with paper strips and an electrode. Calcium concentrations in stimulated saliva were determined with a selective electrode. Group A individuals had inadequate blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 1 diabetes mellitus; Saliva pH; Salivary flow rate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800003

Liposomal photosensitizers: potential platforms for anticancer photodynamic therapy
Provedor de dados: 56
Autores: Muehlmann,L.A.; Joanitti,G.A.; Silva,J.R.; Longo,J.P.F.; Azevedo,R.B.
Photodynamic therapy is a well-established and clinically approved treatment for several types of cancer. Antineoplastic photodynamic therapy is based on photosensitizers, i.e., drugs that absorb photons translating light energy into a chemical potential that damages tumor tissues. Despite the encouraging clinical results with the approved photosensitizers available today, the prolonged skin phototoxicity, poor selectivity for diseased tissues, hydrophobic nature, and extended retention in the host organism shown by these drugs have stimulated researchers to develop new formulations for photodynamic therapy. In this context, due to their amphiphilic characteristic (compatibility with both hydrophobic and hydrophilic substances), liposomes have proven to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Liposome; Antineoplastic therapy; Cancer; Photosensitizer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800001

Propofol inhibits proliferation, migration, and invasion but promotes apoptosis by regulation of Sox4 in endometrial cancer cells
Provedor de dados: 56
Autores: Du,Qing; Liu,Jia; Zhang,Xuezhi; Zhang,Xin; Zhu,He; Wei,Ming; Wang,Shilei.
Propofol is an intravenous sedative hypnotic agent of which the growth-inhibitory effect has been reported on various cancers. However, the roles of propofol in endometrial cancer (EC) remain unclear. This study aimed to explore the effects of propofol on EC in vitro and in vivo. Different concentrations of propofol were used to treat Ishikawa cells. Colony number, cell viability, cell cycle, apoptosis, migration, and invasion were analyzed by colony formation, MTT, flow cytometry, and Transwell assays. In addition, the pcDNA3.1-Sox4 and Sox4 siRNA plasmids were transfected into Ishikawa cells to explore the relationship between propofol and Sox4 in EC cell proliferation. Tumor weight in vivo was measured by xenograft tumor model assay. Protein levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endometrial cancer; Propofol; Cell proliferation; Apoptosis; Sox4; Wnt/β-catenin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400605

Ropinirole alters gene expression profiles in SH-SY5Y cells: a whole genome microarray study
Provedor de dados: 56
Autores: Zhu,M.Z.; Le,W.D.; Jin,G.
Ropinirole (ROP) is a dopamine agonist that has been used as therapy for Parkinson's disease. In the present study, we aimed to detect whether gene expression was modulated by ROP in SH-SY5Y cells. SH-SY5Y cell lines were treated with 10 µM ROP for 2 h, after which total RNA was extracted for whole genome analysis. Gene expression profiling revealed that 113 genes were differentially expressed after ROP treatment compared with control cells. Further pathway analysis revealed modulation of the phosphatidylinositol 3-kinase (PI3K) signaling pathway, with prominent upregulation of PIK3C2B. Moreover, batches of regulated genes, including PIK3C2B, were found to be located on chromosome 1. These findings were validated by quantitative RT-PCR and Western blot...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson's disease; Gene expression; Ropinirole; SH-SY5Y.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200607

Breathing pattern and thoracoabdominal motion during exercise in chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Alves,G.S.; Britto,R.R.; Campos,F.C.; Vilaça,A.B.O.; Moraes,K.S.; Parreira,V.F.
Subjects with chronic obstructive pulmonary disease (COPD) present breathing pattern and thoracoabdominal motion abnormalities that may contribute to exercise limitation. Twenty-two men with stable COPD (FEV1 = 42.6 ± 13.5% predicted; age 68 ± 8 years; mean ± SD) on usual medication and with at least 5 years of diagnosis were evaluated at rest and during an incremental cycle exercise test (10 watts/2 min). Changes in respiratory frequency, tidal volume, rib cage and abdominal motion contribution to tidal volume and the phase angle that measures the asynchrony were analyzed by inductive respiratory plethysmography at rest and during three levels of exercise (30-50, 70-80, and 100% maximal work load). Repeated measures ANOVA followed by pre-planned contrasts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breathing pattern; Chronic obstructive pulmonary disease; Exercise; Plethysmography; Ventilation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100001

Vitamin D3 levels and NLRP3 expression in murine models of obese asthma: association with asthma outcomes
Provedor de dados: 56
Autores: Zhang,J-H.; Chen,Y-P.; Yang,X.; Li,C-Q.
Vitamin D (25(OH)D3) is an essential nutrient that plays a role in the immune system. Serum 25(OH)D3 is found to be associated with asthma. However, the role of vitamin D in obese asthma remains unclear. Therefore, we investigated the association between vitamin D levels and asthma outcomes in a murine model of obese asthma. We also evaluated NLRP3 inflammasome activity in the pathogenesis of obese asthma. We divided 20 male Balb/c mice (3–4 weeks old) into 4 groups: normal control, asthma, obese, and obese asthma and developed an obese asthma mouse model. Airway hyperreactivity, cytokine concentrations, 25(OH)D3 levels, NLRP3 mRNA and IL-1β mRNA expressions were measured. Lung histology and bronchoalveolar lavage fluid (BALF) cell count were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Obesity; Airway hyperreactivity; NLRP3 inflammasome; Vitamin D3.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100612

Modulatory effects of taurine on jejunal contractility
Provedor de dados: 56
Autores: Yao,Q.Y.; Chen,D.P.; Ye,D.M.; Diao,Y.P.; Lin,Y.
Taurine (2-aminoethanesulfonic acid) is widely distributed in animal tissues and has diverse pharmacological effects. However, the role of taurine in modulating smooth muscle contractility is still controversial. We propose that taurine (5-80 mM) can exert bidirectional modulation on the contractility of isolated rat jejunal segments. Different low and high contractile states were induced in isolated jejunal segments of rats to observe the effects of taurine and the associated mechanisms. Taurine induced stimulatory effects on the contractility of isolated rat jejunal segments at 3 different low contractile states, and inhibitory effects at 3 different high contractile states. Bidirectional modulation was not observed in the presence of verapamil or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taurine; Bidirectional modulation; Enteric nervous system; Calcium dependent; Homeostasis; Intestinal motility.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201068

Alternative pathways for angiotensin II generation in the cardiovascular system
Provedor de dados: 56
Autores: Becari,C.; Oliveira,E.B.; Salgado,M.C.O.
The classical renin-angiotensin system (RAS) consists of enzymes and peptides that regulate blood pressure and electrolyte and fluid homeostasis. Angiotensin II (Ang II) is one of the most important and extensively studied components of the RAS. The beneficial effects of angiotensin converting enzyme (ACE) inhibitors in the treatment of hypertension and heart failure, among other diseases, are well known. However, it has been reported that patients chronically treated with effective doses of these inhibitors do not show suppression of Ang II formation, suggesting the involvement of pathways alternative to ACE in the generation of Ang II. Moreover, the finding that the concentration of Ang II is preserved in the kidney, heart and lungs of mice with an ACE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Angiotensin II; Rat elastase-2; Alternative pathway; Angiotensin-converting enzyme.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900013

Short-term evaluation of non-absorbable microgranular hydroxyapatite infiltration in the guinea pig subepidermal abdominal region
Provedor de dados: 56
Autores: Xavier,A.P.; Schellini,S.A.; Aragon,F.F.; Padovani,C.R.; Taga,E.M.
Non-absorbable microgranular hydroxyapatite was infiltrated into the subepidermal abdominal region of guinea pigs in order to assess the possibility of using this material to correct deficiencies in orbital volume. Microgranular hydroxyapatite (2.0 ml) was subepidermally infiltrated into the abdominal region of 20 guinea pigs. The animals were divided into four experimental groups of 5 animals each, which were killed 7 (G1), 15 (G2), 30 (G3) and 60 (G4) days after infiltration. The area and the largest and smallest diameters of the nodules formed by infiltration were evaluated at the site of infiltration and histological examination was performed. The mean granuloma area was similar in all groups. Histopathological examination showed that the material...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infiltration; Microgranular hydroxyapatite; Guinea pigs; Histopathology.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100010

Protective roles of pulmonary rehabilitation mixture in experimental pulmonary fibrosis in vitro and in vivo
Provedor de dados: 56
Autores: Zhang,L.; Ji,Y.X.; Jiang,W.L.; Lv,C.J.
Abnormal high mobility group protein B1 (HMGB1) activation is involved in the pathogenesis of pulmonary fibrosis. Pulmonary rehabilitation mixture (PRM), which combines extracts from eight traditional Chinese medicines, has very good lung protection in clinical use. However, it is not known if PRM has anti-fibrotic activity. In this study, we investigated the effects of PRM on transforming growth factor-β1 (TGF-β1)-mediated and bleomycin (BLM)-induced pulmonary fibrosis in vitro and in vivo. The effects of PRM on TGF-β1-mediated epithelial-mesenchymal transition (EMT) in A549 cells, on the proliferation of human lung fibroblasts (HLF-1) in vitro, and on BLM-induced pulmonary fibrosis in vivo were investigated. PRM treatment resulted in a reduction of EMT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High mobility group protein B1; Epithelial-mesenchymal transition; Pulmonary rehabilitation mixture; Receptor for advanced glycation end-product.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600545

Differential activity of a lectin from Solieria filiformis against human pathogenic bacteria
Provedor de dados: 56
Autores: Holanda,M.L.; Melo,V.M.M.; Silva,L.M.C.M.; Amorim,R.C.N.; Pereira,M.G.; Benevides,N.M.B.
A lectin isolated from the red alga Solieria filiformis was evaluated for its effect on the growth of 8 gram-negative and 3 gram-positive bacteria cultivated in liquid medium (three independent experiments/bacterium). The lectin (500 µg/mL) stimulated the growth of the gram-positive species Bacillus cereus and inhibited the growth of the gram-negative species Serratia marcescens, Salmonella typhi, Klebsiella pneumoniae, Enterobacter aerogenes, Proteus sp, and Pseudomonas aeruginosa at 1000 µg/mL but the lectin (10-1000 µg/mL) had no effect on the growth of the gram-positive bacteria Staphylococcus aureus and B. subtilis, or on the gram-negative bacteria Escherichia coli and Salmonella typhimurium. The purified lectin significantly reduced the cell density...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Red alga; Solieria filiformis; Human pathogenic bacteria; Lectin; Antibacterial activity; Klebsiella pneumoniae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200005

Sex differences in angiotensin II- induced hypertension
Provedor de dados: 56
Autores: Xue,B.; Johnson,A.K.; Hay,M.
Sex differences in the development of hypertension and cardiovascular disease have been described in humans and in animal models. In this paper we will review some of our studies which have as their emphasis the examination of the role of sex differences and sex steroids in modulating the central actions of angiotensin II (ANG II) via interactions with free radicals and nitric oxide, generating pathways within brain circumventricular organs and in central sympathomodulatory systems. Our studies indicate that low-dose infusions of ANG II result in hypertension in wild-type male mice but not in intact wild-type females. Furthermore, we have demonstrated that ANG II-induced hypertension in males is blocked by central infusions of the androgen receptor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Estrogen; Estrogen a and ß receptors; Nitric oxide; Reactive oxygen species; Testosterone.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500018

Urinary secretory IgA after nutritional rehabilitation
Provedor de dados: 56
Autores: Teodósio,M.R.; Oliveira,E.C.M.
We studied the secretory IgA (sIgA) response of the mucosal urinary tract of malnourished children before and after nutritional rehabilitation. sIgA concentration (mg/l) was determined by ELISA in 187 children aged 3 months to 5 years. The children, who frequented a day care center, were divided into four groups, according to nutritional status: 57 were eutrophic, 49 were undergrown, 57 were moderately malnourished and 24 were severely malnourished. In addition, dip slide (Urotube, Roche) and dip-stick (Combur 9-Boehringer) tests showed that children had no bacteriuria or any other urinary abnormalities. Plasma albumin concentration (g/dl) was significantly lower (P&amp;lt;0.005) in the severely malnourished group (mean 3.0 ± 0.3 SD) than in the eutrophic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract; Local immune response; Secretory IgA; Nutritional status.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400007

Immunophenotype of normal and leukemic bone marrow B-precursors in a Brazilian population. A comparative analysis by quantitative fluorescence cytometry
Provedor de dados: 56
Autores: Rego,E.M.; Garcia,A.B.; Carneiro,J.J.; Falcão,R.P.
The distinction between normal and leukemic bone marrow (BM) B-precursors is essential for the diagnosis and treatment monitoring of acute lymphoblastic leukemia (ALL). In order to evaluate the potential use of quantitative fluorescence cytometry (QFC) for this distinction, we studied 21 normal individuals and 40 patients with CD10+ ALL. We characterized the age-related changes of the CD10, CD19, TdT, CD34 and CD79a densities in normal and leukemic BM. Compared to normal adults, the B-precursors from normal children expressed significantly lower values of CD34-specific antibody binding capacity (SABC) (median value of 86.6 vs 160.2 arbitrary units (a.u.) in children and adults, respectively). No significant age-related difference was observed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lymphoblastic leukemia; Lymphocytes; Quantitative fluorescence cytometry; Flow cytometry; Age-related changes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200005

Allogeneic hematopoietic stem cell transplantation from an alternative stem cell source in Fanconi anemia patients: analysis of 47 patients from a single institution
Provedor de dados: 56
Autores: Medeiros,C.R. de; Bitencourt,M.A.; Zanis-Neto,J.; Maluf,E.C.P.; Carvalho,D.S.; Bonfim,C.S.; Funke,V.M.; Setubal,D.C.; Farah,N.; Pasquini,R.
We transplanted 47 patients with Fanconi anemia using an alternative source of hematopoietic cells. The patients were assigned to the following groups: group 1, unrelated bone marrow (N = 15); group 2, unrelated cord blood (N = 17), and group 3, related non-sibling bone marrow (N = 15). Twenty-four patients (51%) had complete engraftment, which was not influenced by gender (P = 0.87), age (P = 0.45), dose of cyclophosphamide (P = 0.80), nucleated cell dose infused (P = 0.60), or use of anti-T serotherapy (P = 0.20). Favorable factors for superior engraftment were full HLA compatibility (independent of the source of cells; P = 0.007) and use of a fludarabine-based conditioning regimen (P = 0.046). Unfavorable factors were  &gt; or = 25 transfusions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anemia; Transplant in Fanconi anemia; Alternative donors; Allogeneic hematopoietic stem cell transplantation in Fanconi anemia; Alternative stem cell source.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000005

Effect of histamine H1 and H2 receptor antagonists, microinjected into cerebellar vermis, on emotional memory consolidation in mice
Provedor de dados: 56
Autores: Gianlorenço,A.C.L.; Serafim,K.R.; Canto-de-Souza,A.; Mattioli,R.
This study investigated the effects of histamine H1 or H2 receptor antagonists on emotional memory consolidation in mice submitted to the elevated plus maze (EPM). The cerebellar vermis of male mice (Swiss albino) was implanted using a cannula guide. Three days after recovery, behavioral tests were performed in the EPM on 2 consecutive days (T1 and T2). Immediately after exposure to the EPM (T1), animals received a microinjection of saline (SAL) or the H1 antagonist chlorpheniramine (CPA; 0.016, 0.052, or 0.16 nmol/0.1 µL) in Experiment 1, and SAL or the H2 antagonist ranitidine (RA; 0.57, 2.85, or 5.7 nmol/0.1 µL) in Experiment 2. Twenty-four hours later, mice were reexposed to the EPM (T2) under the same experimental conditions but they did not receive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorpheniramine; Ranitidine; Cerebellar vermis; Emotional memory consolidation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200135

Surfactant protein B gene polymorphism in preterm babies with respiratory distress syndrome
Provedor de dados: 56
Autores: Lyra,P.P.R.; Diniz,E.M.A.; Abe-Sandes,K.; Angelo,A.L.D.; Machado,T.M.B.; Cardeal,M.
The etiology of respiratory distress syndrome (RDS) is multifactorial and multigenic. Studies have suggested that polymorphisms and mutations in the surfactant protein B (SP-B) gene are associated with the pathogenesis of RDS. The objectives of this study were to determine and compare the frequencies of SP-B gene polymorphisms in preterm babies with and without RDS. We studied 151 neonates: 79 preterm babies without RDS and 72 preterm newborns with RDS. The following four SP-B gene polymorphisms were analyzed: A/C at -18, C/T at 1580, A/G at 9306, and G/C at nucleotide 8714. The polymorphisms were detected by PCR amplification of genomic DNA and genotyping. The genotypes were determined using PCR-based converted restriction fragment length polymorphisms....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Newborn respiratory distress syndrome; Genetic polymorphism; Surfactant protein B.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100010

Zinc and water intake in rats: investigation of adrenergic and opiatergic central mechanisms
Provedor de dados: 56
Autores: Fregoneze,J.B.; Luz,C.P.; Castro,L.; Oliveira,P.; Lima,A.K.S.; Souza,F.; Maldonado,I.; Macêdo,D.F.; Ferreira,M.G.; Bandeira,I.P.V.; Rocha Jr.,M.A.; Carvalho,F.L.Q.; De-Castro-e-Silva,E.
We have demonstrated that central administration of zinc in minute amounts induces a significant antidipsogenic action in dehydrated rats as well as in rats under central cholinergic and angiotensinergic stimulation. Here we show that acute third ventricle injections of zinc also block water intake induced by central ß-adrenergic stimulation in Wistar rats (190-250 g). Central inhibition of opioid pathways by naloxone reverses the zinc-induced antidipsogenic effect in dehydrated rats. After 120 min, rats receiving third ventricle injections of isoproterenol (160 nmol/rat) exhibited a significant increase in water intake (5.78 ± 0.54 ml/100 g body weight) compared to saline-treated controls (0.15 ± 0.07 ml/100 g body weight). Pretreatment with zinc (3.0,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Zinc; Isoproterenol; Opioids; Thirst; Naloxone; Rats.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000007

Association of urinary 90 kDa angiotensin- converting enzyme with family history of hypertension and endothelial function in normotensive individuals
Provedor de dados: 56
Autores: Teixeira,A.M.S.; Plavnik,F.L.; Fernandes,F.B.; Marson,O.; Christofalo,D.M.J.; Ajzen,S.A.; Sesso,R.; Franco,M.C.; Casarini,D.E.
We described angiotensin-I-converting enzyme (ACE) isoforms with molecular masses of 190, 90, and 65 kDa in the urine of normotensive offspring of hypertensive subjects. Since they did not appear in equal amounts, we suggested that 90 kDa ACE might be a marker for hypertension. We evaluated the endothelial response in normotensive offspring with or without family history of hypertension and its association with the 90 kDa ACE in urine. Thirty-five normotensive subjects with a known family history of hypertension and 20 subjects without a family history of hypertension, matched for age, sex, body weight, and blood pressure, were included in the study. Endothelial function was assessed by ultrasound and a sample of urine was collected for determination of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelial dysfunction; Angiotensin-I-converting enzyme; Hypertension; Normotension; N-domain angiotensin- converting enzyme.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500002

Functional changes of dendritic cells in hypersensivity reactions to amoxicillin
Provedor de dados: 56
Autores: Lima,C.M.F.; Schroeder,J.T.; Galvão,C.E.S.; Castro,F.M.; Kalil,J.; Adkinson Jr.,N.F.
A better understanding of dendritic cell (DC) involvement in responses to haptenic drugs is needed, because it represents a possible approach to the development of an in vitro test, which could identify patients prone to drug allergies. There are two main DC subsets: plasmacytoid DC (pDC) and myeloid DC (mDC). β-lactams form hapten-carrier conjugates and may provide a suitable model to study DC behavior in drug allergy reactions. It has been demonstrated that drugs interact differently with DC in drug allergic and non-allergic patients, but there are no studies regarding these subsets. Our aim was to assess the functional changes of mDC and pDC harvested from an amoxicillin-hypersensitive 32-year-old woman who experienced a severe maculopapular exanthema...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dendritic cells; Β-lactam hypersensivity; Plasmacytoid dendritic cells; Drug allergy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000007

The nucleolus: a paradigm for cell proliferation and aging
Provedor de dados: 56
Autores: Comai,L.
The nucleolus is the cellular site of ribosome biosynthesis. At this site, active ribosomal DNA (rDNA) genes are rapidly transcribed by RNA polymerase I (pol I) molecules. Recent advances in our understanding of the pol I transcription system have indicated that regulation of ribosomal RNA (rRNA) synthesis is a critical factor in cell growth. Importantly, the same signaling networks that control cell growth and proliferation and are deregulated in cancer appear to control pol I transcription. Therefore, the study of the biochemical basis for growth regulation of pol I transcription can provide basic information about the nuclear signaling network. Hopefully, this information may facilitate the search for drugs that can inhibit the growth of tumor cells by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleolus; RNA pol I; Aging; Transcription; Werner syndrome.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200004

Reduction of intraspecific aggression in adult rats by neonatal treatment with a selective serotonin reuptake inhibitor
Provedor de dados: 56
Autores: Manhães de Castro,R.; Barreto Medeiros,J.M.; Mendes da Silva,C.; Ferreira,L.M.P.; Guedes,R.C.A.; Cabral Filho,J.E.; Costa,J.A.
Most studies suggest that serotonin exerts an inhibitory control on the aggression process. According to experimental evidence, this amine also influences growth and development of the nervous tissue including serotoninergic neurons. Thus, the possibility exists that increased serotonin availability in young animals facilitates a long-lasting effect on aggressive responses. The present study aimed to investigate the aggressive behavior of adult rats (90-120 days) treated from the 1st to the 19th postnatal day with citalopram (CIT), a selective serotonin reuptake inhibitor (20 mg/kg, sc, every 3 days). Aggressive behavior was induced by placing a pair of rats (matched by weight) in a box (20 x 20 x 20 cm), and submitting them to a 20-min session of electric...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aggression; Serotonin; Neonatal treatment; Development.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100015

Exosomes secreted from miRNA-29b-modified mesenchymal stem cells repaired spinal cord injury in rats
Provedor de dados: 56
Autores: Yu,Tao; Zhao,Cunju; Hou,Shouzhi; Zhou,Weijie; Wang,Baoxin; Chen,Yunzhen.
Exosomes, a kind of extracellular vesicle, are promising therapeutic agents for spinal cord injury (SCI). This article aimed to investigate effects of exosomes secreted from miRNA-29b-modified bone marrow mesenchymal stem cells (BMSCs) on SCI. Exosomes were extracted from BMSCs transfected with miRNA-29b or negative control (miR NC). SCI rats were injected intravenously with exosomes (control exosomes, miRNA-29b exosomes) and BMSCs (miR NC, miRNA-29b) through the tail vein. The expression of miRNA-29b in spinal cord tissues of SCI rats was detected by qRT-PCR. The hind limb motor function was evaluated by Basso Beattie Bresnahan (BBB) score. The histopathological damage and neuronal regeneration in spinal cord tissues was observed by HE staining and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiRNA-29b; Exosomes; Spinal cord injury; BMSCs.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200614

Insulin receptor has tyrosine kinase activity toward Shc in rat liver
Provedor de dados: 56
Autores: Páez-Espinosa,E.V.; Carvalho,C.R.O.; Velloso,L.A.; Saad,M.J.A.
Insulin induces tyrosine phosphorylation of Shc in cell cultures and in insulin-sensitive tissues of the intact rat. However, the ability of insulin receptor (IR) tyrosine kinase to phosphorylate Shc has not been previously demonstrated. In the present study, we investigated insulin-induced IR tyrosine kinase activity towards Shc. Insulin receptor was immunoprecipitated from liver extracts, before and after a very low dose of insulin into the portal vein, and incubated with immunopurified Shc from liver of untreated rats. The kinase assay was performed in vitro in the presence of exogenous ATP and the phosphorylation level was quantified by immunoblotting with antiphosphotyrosine antibody. The results demonstrate that Shc interacted with insulin receptor...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tyrosine kinase activity; Insulin receptor; Shc; Insulin action.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100008

Immunohistochemical characterization of the chick marginal retina
Provedor de dados: 56
Autores: Lima,I.P.; Saito,K.; Hamassaki,D.E.; Yan,C.Y.I.
The retina is a highly differentiated tissue with a complex layered structure that has been extensively characterized. However, most of the previous studies focused on the histology of the central retina while little is known about the cellular composition, organization and function of the marginal retina. Recent research has identified a subpopulation of multipotential progenitor cells in the marginal regions of the retina, closest to the ciliary body ("ciliary marginal zone"). These cells are capable of differentiation in response to an appropriate stimulus. Thus, it is possible that the structure and composition of the marginal retina are distinct from those of the central retina to accommodate the potential addition of newly formed neurons. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marginal retina; Ciliary marginal zone; Calbindin; Calretinin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100006

Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARγ expression
Provedor de dados: 56
Autores: Jasso-Villagomez,E.I.; Garcia-Lorenzana,M.; Almanza-Perez,J.C.; Fortis-Barrera,M.A.; Blancas-Flores,G.; Roman-Ramos,R.; Prado-Barragan,L.A.; Alarcon-Aguilar,F.J.
Ulomoides dermestoides is a beetle traditionally consumed to treat diabetes. In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Normal mice and alloxan-induced diabetic mice were administered fractions of chitin, protein or fat, and the acute hypoglycemic effect was evaluated. A subacute study involving daily administration of these fractions to diabetic mice was also performed over 30 days, after which the liver and pancreas were processed by conventional histological techniques and stained with hematoxylin and eosin to evaluate morphological changes. The most active fraction, the fat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Ulomoides dermestoides; Hypoglycemic agent; Insulin-sensitizing agent; Fatty acids; PPARγ.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600606

Heart rate recovery after exercise: relations to heart rate variability and complexity
Provedor de dados: 56
Autores: Javorka,M.; Zila,I.; Balhárek,T.; Javorka,K.
Physical exercise is associated with parasympathetic withdrawal and increased sympathetic activity resulting in heart rate increase. The rate of post-exercise cardiodeceleration is used as an index of cardiac vagal reactivation. Analysis of heart rate variability (HRV) and complexity can provide useful information about autonomic control of the cardiovascular system. The aim of the present study was to ascertain the association between heart rate decrease after exercise and HRV parameters. Heart rate was monitored in 17 healthy male subjects (mean age: 20 years) during the pre-exercise phase (25 min supine, 5 min standing), during exercise (8 min of the step test with an ascending frequency corresponding to 70% of individual maximal power output) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise recovery; Heart rate variability; Entropy; Spectral analysis; Parasympathetic system.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800018

Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression
Provedor de dados: 56
Autores: Regatieri,C.V.; Dreyfuss,J.L.; Melo,G.B.; Lavinsky,D.; Hossaka,S.K.; Rodrigues,E.B.; Farah,M.E.; Maia,M.; Nader,H.B.
The objective of the present study was to develop a quantitative method to evaluate laser-induced choroidal neovascularization (CNV) in a rat model using Heidelberg Retina Angiograph 2 (HRA2) imaging. The expression of two heparan sulfate proteoglycans (HSPG) related to inflammation and angiogenesis was also investigated. CNV lesions were induced with argon laser in 21 heterozygous Zucker rats and after three weeks a fluorescein angiogram and autofluorescence exams were performed using HRA2. The area and greatest linear dimension were measured by two observers not aware of the protocol. Bland-Altman plots showed agreement between the observers, suggesting that the technique was reproducible. After fluorescein angiogram, HSPG (perlecan and syndecan-4) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Choroidal neovascularization; Fluorescein angiogram; Proteoglycans; Angiogenesis; Confocal scanning laser ophthalmoscope.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700004

Targeting PPM1D by lentivirus-mediated RNA interference inhibits the tumorigenicity of bladder cancer cells
Provedor de dados: 56
Autores: Wang,W.; Zhu,H.; Zhang,H.; Zhang,L.; Ding,Q.; Jiang,H.
Protein phosphatase magnesium/manganese-dependent 1D (PPM1D) is a p53-induced phosphatase that functions as a negative regulator of stress response pathways and has oncogenic properties. However, the functional role ofPPM1D in bladder cancer (BC) remains largely unknown. In the present study, lentivirus vectors carrying small hairpin RNA (shRNA) targeting PPM1D were used to explore the effects ofPPM1D knockdown on BC cell proliferation and tumorigenesis. shRNA-mediated knockdown of PPM1D significantly inhibited cell growth and colony forming ability in the BC cell lines 5637 and T24. Flow cytometric analysis showed that PPM1D silencing increased the proportion of cells in the G0/G1 phase. Downregulation of PPM1Dalso inhibited 5637 cell tumorigenicity in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein phosphatase magnesium/manganese-dependent 1D; Bladder cancer; Gene silencing; RNA interference; Proliferation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201044

Resistance of multicellular aggregates to pharmorubicin observed in human hepatocarcinoma cells
Provedor de dados: 56
Autores: Jianmin,Z.; Hongfang,W.; Meifu,F.
The objective of the present study was to investigate the multicellular resistance of human hepatocarcinoma cells BEL-7402 to pharmorubicin. Cells (1 x 10(4)) and 200 microcarrier Cytodex-3 beads were seeded onto a 24-well plate and cultured in RPMI 1640 medium. After the formation of multicellular aggregates, morphology and cell viability were analyzed by scanning electron microscopy, transmission electron microscopy and flow cytometry, respectively. The IC50 was determined by flow cytometry and MTT assay after the cells cultured in aggregates and monolayers were treated with pharmorubicin. The culture products exhibited structural characteristics somewhat similar to those of trabecular hepatocarcinoma in vivo. Among the microcarriers, cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Spheroids; Drug resistance; Microcarrier; Epirubicin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200015

Anxiogenic-like effect of acute and chronic fluoxetine on rats tested on the elevated plus-maze
Provedor de dados: 56
Autores: Silva,M.T.A.; Alves,C.R.R.; Santarem,E.M.M.
The selective serotonin reuptake inhibitor fluoxetine (FLX) is widely prescribed for depression and anxiety-related disorders. On the other hand, enhanced serotonergic transmission is known to be classically related to anxiety. In this study, the effects of acute (5.0 mg/kg) and chronic (5.0 mg/kg, 22 days) FLX were investigated in both food-deprived and non-deprived rats tested in the elevated plus-maze. Significant main effects of the three factors (drug, food condition and administration regimen) were observed, but no interaction between them. The administration of either acute or chronic FLX resulted in an anxiogenic effect, as detected by a significant reduction in the percentage of time spent in the open arms and in the percentage of open arm...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elevated plus-maze; Fluoxetine; Anxiety; Handling; Food-restriction.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300014

Kaempferol suppresses human gastric cancer SNU-216 cell proliferation, promotes cell autophagy, but has no influence on cell apoptosis
Provedor de dados: 56
Autores: Zhang,Fan; Ma,Cuimei.
Gastric cancer remains a serious threat to human health worldwide. Kaempferol is a plant-derived flavonoid compound with a wide range of pharmacological activities. This study aimed to investigate the effects of kaempferol on gastric cancer SNU-216 cell proliferation, apoptosis, and autophagy, as well as underlying potential mechanisms. Viability, proliferation, and apoptosis of SNU-216 cells after kaempferol treatment were evaluated using cell counting kit-8 assay, 5-btomo-2′-deoxyuridine incorporation assay, and annexin V-FITC/PI staining, respectively. Quantitative reverse transcription PCR was performed to measure the mRNA expressions of cyclin D1 and microRNA-181a (miR-181a) in SNU-216 cells. Cell transfection was used to down-regulate the expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric cancer; Kaempferol; MicroRNA-181a; Cell proliferation; Cell autophagy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200610

Patterns of cerebral activation during lexical and phonological reading in Portuguese
Provedor de dados: 56
Autores: Senaha,M.L.H.; Martin,M.G.M.; Amaro Jr.,E.; Campi,C.; Caramelli,P.
According to the concepts of cognitive neuropsychology, there are two principal routes of reading processing: a lexical route, in which global reading of words occurs and a phonological route, responsible for the conversion of the graphemes into their respective phonemes. In the present study, functional magnetic resonance imaging (fMRI) was used to investigate the patterns of cerebral activation in lexical and phonological reading by 13 healthy women with a formal educational level greater than 11 years. Participants were submitted to a silent reading task containing three types of stimuli: real words (irregular and foreign words), nonwords and illegitimate graphic stimuli. An increased number of activated voxels were identified by fMRI in the word...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional magnetic resonance imaging; Reading; Lexical processing; Phonological processing; Cerebral activation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200013

Inflammation induced by increased frequency of intermittent hypoxia is attenuated by tempol administration
Provedor de dados: 56
Autores: Zhang,J.; Zheng,L.; Cao,J.; Chen,B.; Jin,D.
The levels of serum inflammatory cytokines and the activation of nuclear factor kappa B (NF-κB) and hypoxia inducible factor-1α (HIF-1α) in heart tissues in response to different frequencies of intermittent hypoxia (IH) and the antioxidant tempol were evaluated. Wistar rats (64 males, 200-220 g) were randomly divided into 6 experimental groups and 2 control groups. Four groups were exposed to IH 10, 20, 30, or 40 times/h. The other 2 experimental groups were challenged with IH (30 times/h) plus tempol, either beginning on day 0 (IH30T0) or on day 29 (IH30T29). After 6 weeks of challenge, serum levels of tumor necrosis factor (TNF)-α, intracellular adhesion molecule (ICAM)-1, and interleukin-10 were measured, and western blot analysis was used to detect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obstructive sleep apnea; Intermittent hypoxia; Inflammation; Nuclear factor kappa B; Antioxidant.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201115

Neonatal handling induces anovulatory estrous cycles in rats
Provedor de dados: 56
Autores: Gomes,C.M.; Frantz,P.J.; Sanvitto,G.L.; Anselmo-Franci,J.A.; Lucion,A.B.
Since previous work has shown that stimulation early in life decreases sexual receptiveness as measured by the female lordosis quotient, we suggested that neonatal handling could affect the function of the hypothalamus-pituitary-gonadal axis. The effects of neonatal handling on the estrous cycle and ovulation were analyzed in adult rats. Two groups of animals were studied: intact (no manipulation, N = 10) and handled (N = 11). Pups were either handled daily for 1 min during the first 10 days of life or left undisturbed. At the age of 90 days, a vaginal smear was collected daily at 9:00 a.m. and analyzed for 29 days; at 9:00 a.m. on the day of estrus, animals were anesthetized with thiopental (40 mg/kg, ip), the ovaries were removed and the oviduct was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Stress; Neonatal handling; Estrous cycle; Ovulation; Female rat.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000010

Implications of infectious diseases and the adrenal hypothesis for the etiology of childhood acute lymphoblastic leukemia
Provedor de dados: 56
Autores: Azevedo-Silva,F.; Camargo,B.de; Pombo-de-Oliveira,M.S.
Acute leukemia is the most frequent cancer in children. Recently, a new hypothesis was proposed for the pathogenesis of childhood acute lymphoblastic leukemia (ALL). The so-called "adrenal hypothesis" emphasized the role of endogenous cortisol in the etiology of B-cell precursor ALL. The incidence peak of ALL in children between 3 to 5 years of age has been well documented and is consistent with this view. The adrenal hypothesis proposes that the risk of childhood B-cell precursor ALL is reduced when early childhood infections induce qualitative and quantitative changes in the hypothalamus-pituitary-adrenal axis. It suggests that the increased plasma cortisol levels would be sufficient to eliminate all clonal leukemic cells originating during fetal life....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lymphoblastic leukemia; Childhood infections; Epidemiology; Kala-azar; Adrenal hypothesis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300001

Upregulation of miR-129-5p increases the sensitivity to Taxol through inhibiting HMGB1-mediated cell autophagy in breast cancer MCF-7 cells
Provedor de dados: 56
Autores: Shi,Ying; Gong,Weihua; Lu,Lu; Wang,Yunfeng; Ren,Jingjing.
Although Taxol has improved the survival of cancer patients as a first-line chemotherapeutic agent, an increasing number of patients develop resistance to Taxol after prolonged treatment. The potential mechanisms of cancer cell resistance to Taxol are not completely clear. It has been reported that microRNAs (miRNAs) are involved in regulating the sensitivity of cancer cells to various chemotherapeutic agents. In this study, we aimed to explore the role of miR-129-5p in regulating the sensitivity of breast cancer cells to Taxol. Cell apoptosis and autophagy, and the sensitivity of MCF-7 cells to Taxol were assessed with a series of in vitro assays. Our results showed that the inhibition of autophagy increased the Taxol-induced apoptosis and the sensitivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-129-5p; HMGB1; Autophagy; Taxol; Breast cancer.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100603

Alanyl-glutamine protects the intestinal barrier function in trained rats against the impact of acute exhaustive exercise
Provedor de dados: 56
Autores: Freitas,A.K.L.; Silva,M.T.B.; Silva,C.M.S.; Prata,M.M.G.; Rodrigues,F.A.P.; Siqueira,R.J.B.; Lima,A.A.M.; Santos,A.A.; Havt,A.
Strenuous exercise triggers deleterious effects on the intestinal epithelium, but their mechanisms are still uncertain. Here, we investigated whether a prolonged training and an additional exhaustive training protocol alter intestinal permeability and the putative effect of alanyl-glutamine (AG) pretreatment in this condition. Rats were allocated into 5 different groups: 1) sedentary; 2 and 3) trained (50 min per day, 5 days per week for 12 weeks) with or without 6 weeks oral (1.5 g/kg) AG supplementation; 4 and 5) trained and subjected to an additional exhaustive test protocol with or without oral AG supplementation. Venous blood samples were collected to determine gasometrical indices at the end of the 12-week protocol or after exhaustive test. Lactate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Claudin-2; Intestinal permeability; Lactulose-mannitol test; Physical exercise; Tight junctions.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500602

Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain
Provedor de dados: 56
Autores: Miranda,E.A.; de-Murcia,G.; Ménissier-de-Murcia,J.
A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 109 infected cells in three liters of culture
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PARP; Insect cells; Baculovirus; Overproduction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800002

Lactate-upregulation of lactate oxidation complex-related genes is blunted in left ventricle of myocardial infarcted rats
Provedor de dados: 56
Autores: Gabriel-Costa,D.; Cunha,T.F.; Paixão,N.A.; Fortunato,R.S.; Rego-Monteiro,I.C.C.; Barreto-Chaves,M.L.M.; Brum,P.C.
Lactate modulates the expression of lactate oxidation complex (LOC)-related genes and cardiac blood flow under physiological conditions, but its modulatory role remains to be elucidated regarding pathological cardiac stress. The present study evaluated the effect of lactate on LOC-related genes expression and hemodynamics of hearts submitted to myocardial infarction (MI). Four weeks after MI or sham operation, isolated hearts of male Wistar rats were perfused for 60 min with Na+-lactate (20 mM). As expected, MI reduced cardiac contractility and relaxation with no changes in perfusion. The impaired cardiac hemodynamics were associated with increased reactive oxygen species (ROS) levels (Sham: 19.3±0.5 vs MI: 23.8±0.3 µM), NADPH oxidase (NOX) activity (Sham:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactate; Lactate oxidation complex; Myocardial infarction; Gene expression; Perfusion pressure; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100605

Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats
Provedor de dados: 56
Autores: Gonçalves,R.V.; Mezêncio,J.M.S.; Benevides,G.P.; Matta,S.L.P.; Neves,C.A.; Sarandy,M.M.; Vilela,E.F.
This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm²); group 2 (L30): GaAlAs laser (30 J/cm²); group 3 (L60):...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cicatrization; Laser; Healing ointment; Collagen I and III; Cutaneous wound.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400005

Bone mineral density of the lumbar spine of Brazilian children and adolescents aged 6 to 14 years
Provedor de dados: 56
Autores: Fonseca,A.S.M.; Szejnfeld,V.L.; Terreri,M.T.; Goldenberg,J.; Ferraz,M.B.; Hilário,M.O.E.
The authors performed a study of bone mass in eutrophic Brazilian children and adolescents using dual-energy X-ray absorptiometry (DXA) in order to obtain curves for bone mineral content (BMC) and bone mineral density (BMD) by chronological age and correlate these values with weight and height. Healthy Caucasian children and adolescents, 120 boys and 135 girls, 6 to 14 years of age, residents of São Paulo, Brazil, were selected from the Pediatric Department outpatient clinic of Hospital São Paulo (Universidade Federal de São Paulo). BMC, BMD and the area of the vertebral body of the L2-L4 segment were obtained by DXA. BMC and BMD for the lumbar spine (L2-L4) presented a progressive increase between 6 and 14 years of age in both sexes, with a distribution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone mineral density; Lumbar spine densitometry; Children; Adolescents.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300008

High HTLV-1 proviral load, a marker for HTLV-1 associated myelopathy/tropical spastic paraparesis, is also detected in patients with infective dermatitis associated with HTLV-1
Provedor de dados: 56
Autores: Primo,J.; Siqueira,I.; Nascimento,M.C.F.; Oliveira,M.F.; Farre,L.; Carvalho,E.M.; Bittencourt,A.L.
Salvador (BA, Brazil) is an endemic area for human T-cell lymphotrophic virus type 1 (HTLV-1). The overall prevalence of HTLV-1 infection in the general population has been estimated to be 1.76%. HTLV-1 carriers may develop a variety of diseases such as adult T-cell leukemia/lymphoma, HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and infective dermatitis associated with HTLV-1 (IDH). IDH is a chronic and severe form of childhood exudative and infective dermatitis involving mainly the scalp, neck and ears. It has recently been observed that 30% of patients with IDH develop juvenile HAM/TSP. The replication of HTLV-1 has been reported to be greater in adult HAM/TSP patients than in asymptomatic HTLV-1 carriers. In the current study, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Infective dermatitis associated with HTLV-1; HTLV-1 associated myelopathy/tropical spastic paraparesis; HTLV-1 proviral loads.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800012

Effects of sciatic-conditioned medium on neonatal rat retinal cells in vitro
Provedor de dados: 56
Autores: Torres,P.M.M.; Guilarducci,C.V.V.; Araujo,E.G.
Schwann cells produce and release trophic factors that induce the regeneration and survival of neurons following lesions in the peripheral nerves. In the present study we examined the in vitro ability of developing rat retinal cells to respond to factors released from fragments of sciatic nerve. Treatment of neonatal rat retinal cells with sciatic-conditioned medium (SCM) for 48 h induced an increase of 92.5 ± 8.8% (N = 7 for each group) in the amount of total protein. SCM increased cell adhesion, neuronal survival and glial cell proliferation as evaluated by morphological criteria. This effect was completely blocked by 2.5 µM chelerythrine chloride, an inhibitor of protein kinase C (PKC). These data indicate that PKC activation is involved in the effect...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokines; Neurotrophic factors; Retina; Schwann cells; Sciatic nerve.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100012

Modulation of the expression of the transcription factor Max in rat retinal ganglion cells by a recombinant adeno-associated viral vector
Provedor de dados: 56
Autores: Petrs-Silva,H.; Chiodo,V.; Chiarini,L.B.; Hauswirth,W.W.; Linden,R.
Exclusion of the transcription factor Max from the nucleus of retinal ganglion cells is an early, caspase-independent event of programmed cell death following damage to the optic axons. To test whether the loss of nuclear Max leads to a reduction in neuroprotection, we developed a procedure to overexpress Max protein in rat retinal tissue in vivo. A recombinant adeno-associated viral vector (rAAV) containing the max gene was constructed, and its efficiency was confirmed by transduction of HEK-293 cells. Retinal ganglion cells were accessed in vivo through intravitreal injections of the vector in rats. Overexpression of Max in ganglion cells was detected by immunohistochemistry at 2 weeks following rAAV injection. In retinal explants, the preparation of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Retina; Max expression; Transcription factor; Viral vector; Gene therapy; Neuroprotection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300008

Relationship between ventilation heterogeneity and exercise intolerance in adults with sickle cell anemia
Provedor de dados: 56
Autores: Lopes,A.J.; Marinho,C.L.; Alves,U.D.; Gonçalves,C.E.A.; Silva,P.O.; Botelho,E.C.; Bedirian,R.; Soares,A.R.; Maioli,M.C.P.
Sickle cell anemia (SCA) causes dysfunction of multiple organs, with pulmonary involvement as a major cause of mortality. Recently, there has been growing interest in the nitrogen single-breath washout (N2SBW) test, which is able to detect ventilation heterogeneity and small airway disease when the results of other pulmonary function tests (PFTs) are still normal. Thus, the objectives of the present study were to assess the heterogeneity in the ventilation distribution in adults with SCA and to determine the association between the ventilation distribution and the clinical, cardiovascular, and radiological findings. This cross-sectional study included 38 adults with SCA who underwent PFTs, echocardiography, computed tomography (CT), and 6-min walk test. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell anemia; Respiratory mechanics; Respiratory function tests; Ventilation; Exercise.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800607

Unusual association of NDM-1 with KPC-2 and armA among Brazilian Enterobacteriaceae isolates
Provedor de dados: 56
Autores: Quiles,M.G.; Rocchetti,T.T.; Fehlberg,L.C.; Kusano,E.J.U.; Chebabo,A.; Pereira,R.M.G.; Gales,A.C.; Pignatari,A.C.C.
We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and was associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), a 16S rRNA methylase not previously reported in Brazil, in two distinct strains of Enterobacter cloacae. Our results suggested that the introduction of blaNDM-1 in Brazil has been accompanied by rapid spread, since our isolates showed no genetic relationship.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Enterobacteriaceae; Carbapenem-resistance; Carbapenemases; Methylase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200174

Solubilization of human erythrocyte membranes by ASB detergents
Provedor de dados: 56
Autores: Domingues,C.C.; Malheiros,S.V.P.; de Paula,E.
Understanding the membrane solubilization process and finding effective solubilizing agents are crucial challenges in biochemical research. Here we report results on the interaction of the novel linear alkylamido propyl dimethyl amino propanosulfonate detergents, ASB-14 and ASB-16, with human erythrocyte membranes. An estimation of the critical micelle concentration of these zwitterionic detergents (ASB-14 = 100 µM and ASB-16 = 10 µM) was obtained using electron paramagnetic resonance. The amount of proteins and cholesterol solubilized from erythrocytes by these detergents was then determined. The hemolytic activities of the ASB detergents were assayed and the detergent/lipid molar ratios for the onset of hemolysis (Re sat) and total lysis (Re sol) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolysis; Protein solubilization; Erythrocyte; Zwitterionic detergents; Critical micelle concentration; Cholesterol solubilization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900003

A case of severe glutathione synthetase deficiency with novel GSS mutations
Provedor de dados: 56
Autores: Xia,H.; Ye,J.; Wang,L.; Zhu,J.; He,Z.
Glutathione synthetase deficiency (GSSD) is a rare inborn error of glutathione metabolism with autosomal recessive inheritance. The severe form of the disease is characterized by acute metabolic acidosis, usually present in the neonatal period with hemolytic anemia and progressive encephalopathy. A case of a male newborn infant who had severe metabolic acidosis with high anion gap, hemolytic anemia, and hyperbilirubinemia is reported. A high level of 5-oxoproline was detected in his urine and a diagnosis of generalized GSSD was made. DNA sequence analysis revealed the infant to be compound heterozygous with two mutations, c.738dupG in exon 8 of GSS gene resulting in p.S247fs and a repetitive sequence in exon 3 of GSS gene. Treatment after diagnosis of GSSD...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Glutathione synthetase; 5-oxoprolinuria; Newborn; Metabolism; Mutation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300501

Antidepressant-like effect of Hoodia gordonii in a forced swimming test in mice: evidence for involvement of the monoaminergic system
Provedor de dados: 56
Autores: Citó,M.C.O.; Silva,M.I.G.; Santos,L.K.X.; Fernandes,M.L.; Melo,F.H.C.; Aguiar,J.A.C.; Lopes,I.S.; Sousa,P.B.; Vasconcelos,S.M.M.; Macêdo,D.S.; Sousa,F.C.F.
Hoodia gordonii is a plant species used traditionally in southern Africa to suppress appetite. Recently, it has been associated with a significant increase in blood pressure and pulse rate in women, suggesting sympathomimetic activity. The present study investigated the possible antidepressant-like effects of acute and repeated (15 days) administration of H. gordonii extract (25 and 50 mg/kg, po) to mice exposed to a forced swimming test (FST). Neurochemical analysis of brain monoamines was also carried out to determine the involvement of the monoaminergic system on these effects. Acute administration of H. gordonii decreased the immobility of mice in the FST without accompanying changes in general activity in the open-field test during acute treatment,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hoodia gordonii; Antidepressant action; Monoaminergic system; Forced swimming test.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100057

Central injection of captopril inhibits the blood pressure response to intracerebroventricular choline
Provedor de dados: 56
Autores: Isbil-Buyukcoskun,N.; Gulec,G.; Ozluk,K.; Ulus,I.H.
In the present study, we investigated the involvement of the brain renin-angiotensin system in the effects of central cholinergic stimulation on blood pressure in conscious, freely moving normotensive rats. In the first step, we determined the effects of intracerebroventricular (icv) choline (50, 100 and 150 µg) on blood pressure. Choline increased blood pressure in a dose-dependent manner. In order to investigate the effects of brain renin-angiotensin system blockade on blood pressure increase induced by choline (150 µg, icv), an angiotensin-converting enzyme inhibitor, captopril (25 and 50 µg, icv), was administered 3 min before choline. Twenty-five µg captopril did not block the pressor effect of choline, while 50 µg captopril blocked it significantly....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Choline; Captopril; Intracerebroventricular choline; Blood pressure.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600018

Immunological basis of septal fibrosis of the liver in Capillaria hepatica-infected rats
Provedor de dados: 56
Autores: Lemos,Q.T.; Magalhães-Santos,I.F.; Andrade,Z.A.
Rats infected with the helminth Capillaria hepatica regularly develop septal fibrosis of the liver similar to that induced by repeated ip injections of pig serum. Fibrosis starts when the focal parasitic lesions begin to show signs of resorption, thus suggesting an immunologically mediated pathogenesis of this fibrosis. To explore this possibility, the development of C. hepatica-related hepatic fibrosis was observed in rats exposed to worm antigens from the first neonatal day onward. Wistar rats (150 g) were either injected ip with an extract of C. hepatica eggs (protein concentration: 1 mg/ml) or received immature eggs by gavage from the first neonatal day until adult life and were then infected with 500 embryonated eggs. Changes were monitored on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capillaria hepatica model; Hepatic fibrosis; Immunological tolerance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900009

Effect of photodynamic therapy on the extracellular matrix and associated components
Provedor de dados: 56
Autores: Pazos,M.d.C.; Nader,H.B.
In many countries, photodynamic therapy (PDT) has been recognized as a standard treatment for malignant conditions (for example, esophageal and lung cancers) and non-malignant ones such as age-related macular degeneration and actinic keratoses. The administration of a non-toxic photosensitizer, its selective retention in highly proliferating cells and the later activation of this molecule by light to form reactive oxygen species that cause cell death is the principle of PDT. Three important mechanisms are responsible for the PDT effectiveness: a) direct tumor cell kill; b) damage of the tumor vasculature; c) post-treatment immunological response associated with the leukocyte stimulation and release of many inflammatory mediators like cytokines, growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Extracellular matrix; Adhesion; Chemical mediators; Matrix metalloproteinases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800002

Assessment of fetal risk associated with exposure to cancer chemotherapy during pregnancy: a multicenter study
Provedor de dados: 56
Autores: Peres,R.M.; Sanseverino,M.T.V.; Guimarães,J.L.M.; Coser,V.; Giuliani,L.; Moreira,R.K.; Ornsten,T.; Schüler-Faccini,L.
The objective of the present study was to evaluate and quantify fetal risks involved in the administration of cancer chemotherapy during gestation, as well as to assess the long-term effects on the exposed children. In this retrospective, cohort study, we reviewed the records of women aged 15 to 45 years with a diagnosis of malignancy or benign tumors with malignant behavior at three reference services in the State of Rio Grande do Sul, Brazil, from 1990 to 1997. All patients with a diagnosis of pregnancy at any time during the course of the disease were selected, regardless of whether or not they received specific medication. Fetal outcomes of 14 pregnancies with chemotherapy exposure were compared to that of 15 control pregnancies in which these drugs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Pregnancy; Chemotherapy; Teratogenicity; Fetal outcome.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200007

Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model
Provedor de dados: 56
Autores: Callejas,G.H.; Figueira,R.L.; Gonçalves,F.L.L.; Volpe,F.A.P.; Zuardi,A.W.; Crippa,J.A.; Hallak,J.E.; Sbragia,L.
Gastroschisis (GS) is an abdominal wall defect that results in histological and morphological changes leading to intestinal motility perturbation and impaired absorption of nutrients. Due to its anti-inflammatory, antioxidant, and neuroprotective effects, cannabidiol (CBD) has been used as a therapeutic agent in many diseases. Our aim was to test the effect of maternal CBD in the intestine of an experimental model of GS. Pregnant rats were treated over 3 days with CBD (30 mg/kg) after the surgical induction of GS (day 18.5 of gestation) and compared to controls. Fetuses were divided into 4 groups: 1) control (C); 2) C+CBD (CCBD); 3) gastroschisis (G), and 4) G+CBD (GCBD). On day 21.5 of gestation, the fetuses were harvested and evaluated for: a) body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroschisis; Experimental model; Prenatal treatment; Cannabidiol.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500607

Effects of high-intensity intermittent training on carnitine palmitoyl transferase activity in the gastrocnemius muscle of rats
Provedor de dados: 56
Autores: Carnevali Jr,L.C.; Eder,R.; Lira,F.S.; Lima,W.P.; Gonçalves,D.C.; Zanchi,N.E.; Nicastro,H.; Lavoie,J.M.; Seelaender,M.C.L.
We examined the capacity of high-intensity intermittent training (HI-IT) to facilitate the delivery of lipids to enzymes responsible for oxidation, a task performed by the carnitine palmitoyl transferase (CPT) system in the rat gastrocnemius muscle. Male adult Wistar rats (160-250 g) were randomly distributed into 3 groups: sedentary (Sed, N = 5), HI-IT (N = 10), and moderate-intensity continuous training (MI-CT, N = 10). The trained groups were exercised for 8 weeks with a 10% (HI-IT) and a 5% (MI-CT) overload. The HI-IT group presented 11.8% decreased weight gain compared to the Sed group. The maximal activities of CPT-I, CPT-II, and citrate synthase were all increased in the HI-IT group compared to the Sed group (P &lt; 0.01), as also was gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-intensity intermittent training; Lipid metabolism; Carnitine palmitoyl transferase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800013

The carotid body of the spontaneous insulin-dependent diabetic rat
Provedor de dados: 56
Autores: Clarke,J.A.; Daly,M. de B.; Ead,H.W.; Hennessy,E.M.
The carotid bodies from adult spontaneous insulin-dependent diabetic rats (strain BB/S) were perfusion-fixed at normal arterial blood pressure with 3% phosphate-buffered glutaraldehyde and compared with the organs from control rats (strain BB/Sc) prepared in the same way. Serial 5-µm sections were cut, stained, and using an interactive image analysis system, were analysed to determine the volumes of the carotid body and its vascular and extravascular compartments. There was no evidence of systemic arterial disease in the carotid stem arteries in either group of animals, and the microvasculature of the organs appeared normal by light microscopy. The volume of the carotid body was unchanged 3 months after the onset of diabetes but was increased at 6 months....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetic rat; Carotid body; Quantitative morphology; Vascular compartment.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100013

Medical applications of shape memory alloys
Provedor de dados: 56
Autores: Machado,L.G.; Savi,M.A.
Shape memory alloys (SMA) are materials that have the ability to return to a former shape when subjected to an appropriate thermomechanical procedure. Pseudoelastic and shape memory effects are some of the behaviors presented by these alloys. The unique properties concerning these alloys have encouraged many investigators to look for applications of SMA in different fields of human knowledge. The purpose of this review article is to present a brief discussion of the thermomechanical behavior of SMA and to describe their most promising applications in the biomedical area. These include cardiovascular and orthopedic uses, and surgical instruments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shape memory alloys; Biomaterials.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600001

Relationship between salt consumption measured by 24-h urine collection and blood pressure in the adult population of Vitória (Brazil)
Provedor de dados: 56
Autores: Rodrigues,S.L.; Souza Júnior,P.R.; Pimentel,E.B.; Baldo,M.P.; Malta,D.C.; Mill,J.G.; Szwarcwald,C.L.
High salt intake is related to an increase in blood pressure and development of hypertension. However, currently, there are no national representative data in Brazil using the gold standard method of 24-h urine collection to measure sodium consumption. This study aimed to determine salt intake based on 24-h urine collection in a sample of 272 adults of both genders and to correlate it with blood pressure levels. We used a rigorous protocol to assure an empty bladder prior to initiating urine collection. We excluded subjects with a urine volume &lt;500 mL, collection period outside of an interval of 23-25 h, and subjects with creatinine excretion that was not within the range of 14.4-33.6 mg/kg (men) and 10.8-25.2 mg/kg (women). The mean salt intake was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sodium; Salt intake; Blood pressure; Hypertension; 24-h urine collection; Population-based study.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800728

DNA tests probe the genomic ancestry of Brazilians
Provedor de dados: 56
Autores: Pena,S.D.J.; Bastos-Rodrigues,L.; Pimenta,J.R.; Bydlowski,S.P.
We review studies from our laboratories using different molecular tools to characterize the ancestry of Brazilians in reference to their Amerindian, European and African roots. Initially we used uniparental DNA markers to investigate the contribution of distinct Y chromosome and mitochondrial DNA lineages to present-day populations. High levels of genetic admixture and strong directional mating between European males and Amerindian and African females were unraveled. We next analyzed different types of biparental autosomal polymorphisms. Especially useful was a set of 40 insertion-deletion polymorphisms (indels) that when studied worldwide proved exquisitely sensitive in discriminating between Amerindians, Europeans and Sub-Saharan Africans. When applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA tests; Insertion-deletion polymorphisms; Ancestry; Human diversity; Brazilians.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000001

Extracellular matrix: understanding the complexity
Provedor de dados: 56
Autores: Borojevic,R.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Extracellular matrix glycoconjugate adhesion; Cell differentiation cell proliferation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500001

Changes in the hormone and lipid profile of obese adolescent Saudi females with acne vulgaris
Provedor de dados: 56
Autores: Abulnaja,K.O.
Acne vulgaris is a multifactorial disease affecting a majority of the adolescent population. The objective of this study was to test for a correlation between fasting serum lipid profiles and levels of testosterone, insulin, leptin, and interleukin 1-β (IL-1β) and the incidence of severe acne vulgaris in obese adolescent females. Four groups of adolescent females were studied: obese with acne, obese without acne, non-obese with acne, and non-obese without acne. Obese females with acne, compared to obese females without acne and non-obese subjects, had significantly higher serum triglycerides, low-density lipoprotein cholesterol and apolipoprotein-B (apo-B) (mean ± SD: 197 ± 13.7 vs 171 ± 11.5, 128 ± 8.3 vs 116 ± 7.7, 96 ± 13.7 vs 85 ± 10.3 mg/dL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acne vulgaris; Obesity; Testosterone; Adolescent females; Serum IL-1β.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600005

Neuronal and endothelial nitric oxide synthase gene knockout mice
Provedor de dados: 56
Autores: Huang,P.L.
Targeted disruption of the neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS) genes has led to knockout mice that lack these isoforms. These animal models have been useful to study the roles of nitric oxide (NO) in physiologic processes. nNOS knockout mice have enlarged stomachs and defects in the inhibitory junction potential involved in gastrointestinal motility. eNOS knockout mice are hypertensive and lack endothelium-derived relaxing factor activity. When these animals are subjected to models of focal ischemia, the nNOS mutant mice develop smaller infarcts, consistent with a role for nNOS in neurotoxicity following cerebral ischemia. In contrast, eNOS mutant mice develop larger infarcts, and show a more pronounced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Targeted disruption; Gene knockout; Endothelium; Stroke; Atherosclerosis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100005

Prevalence of Y chromosome deletions in a Brazilian population of nonobstructive azoospermic and severely oligozoospermic men
Provedor de dados: 56
Autores: SãoPedro,S.L.; Fraietta,R.; Spaine,D.; Porto,C.S.; Srougi,M.; Cedenho,A.P.; Avellar,M.C.W.
We determined the prevalence of Y chromosome deletions in a population of 60 Brazilian nonobstructive azoospermic and severely oligozoospermic men. PCR-based screening of microdeletions was performed on lymphocyte DNA for the presence of 14 sequence-tagged sites (STS) located in the azoospermic factor (AZF) on the Yq chromosome. All STS were amplified efficiently in samples from 12 fertile men tested, but failed to be amplified in samples from fertile women, indicating the specificity of PCR conditions for Yq screening. Overall, 4 of the 60 infertile patients tested (6.7%) exhibited deletion of the Y chromosome, 2 of them being severely oligozoospermic patients (P10 and P32) and 2 azoospermic men (patients P47 and P57). Patients P47 and P57 presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Y chromosome; Microdeletions; Severe oligozoospermia; Azoospermia; Male infertility; Azoospermia factor; AZF region.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600015

Beneficial effect of recombinant human growth hormone on the intestinal mucosa barrier of septic rats
Provedor de dados: 56
Autores: Yi,C.; Cao,Y.; Wang,S.R.; Xu,Y.Z.; Huang,H.; Cui,Y.X.; Huang,Y.
The objective of the present study was to investigate the effects of recombinant human growth hormone (rhGH) on the intestinal mucosa barrier of septic rats and explore its possible mechanism. Female Sprague-Dawley rats were randomized into three groups: control, Escherichia coli-induced sepsis (S) and treatment (T) groups. Groups S and T were subdivided into subgroups 1d and 3d, respectively. Expression of liver insulin-like growth factor-1 (IGF-1) mRNA, Bcl-2 and Bax protein levels and the intestinal Bax/Bcl-2 ratio, and plasma GH and IGF-1 levels were determined. Histological examination of the intestine was performed and bacterial translocation was determined. rhGH significantly attenuated intestinal mucosal injuries and bacterial translocation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal mucosa barrier; Growth hormone; Sepsis; Insulin-like growth factor-1; Apoptosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100006

An optically coupled power stimulus isolation unit with high voltage and fast rise time output
Provedor de dados: 56
Autores: Brasil,R.O.; Leal-Cardoso,J.H.
Recent technological developments have created new devices that could improve and simplify the construction of stimulus isolators. HEXFET transistors can switch large currents and hundreds of volts in nanoseconds. The newer opto-isolators can give a pulse rise time of a few nanoseconds, with output compatible with MOSFET devices, in which delays are reduced to nanoseconds. Integrated DC/DC converters are now available. Using these new resources we developed a new electrical stimulus isolator circuit with selectable constant-current and constant-voltage modes, which are precise and easy to construct. The circuit works like a regulated power supply in both modes with output switched to zero or to free mode through an opto-isolator device. The isolator...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Electrical isolation; Electrical stimulation; Pulse stimulation; Stimulus isolator.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600013

SIRT1 negatively regulates amyloid-beta-induced inflammation via the NF-κB pathway
Provedor de dados: 56
Autores: Cao,L.; Liu,C.; Wang,F.; Wang,H.
Chronic inflammation induced by amyloid-beta (Aβ) plays a key role in the development of age-related macular degeneration (AMD), and matrix metalloproteinase-9 (MMP-9), interleukin (IL)-6, and IL-8 may be associated with chronic inflammation in AMD. Sirtuin 1 (SIRT1) regulates inflammation via inhibition of nuclear factor-kappa B (NF-κB) signaling, and resveratrol has been reported to prevent Aβ-induced retinal degeneration; therefore, we investigated whether this action was mediated via activation of SIRT1 signaling. Human adult retinal pigment epithelial (RPE) cells were exposed to Aβ, and overactivation and knockdown of SIRT1 were performed to investigate whether SIRT1 is required for abrogating Aβ-induced inflammation. We found that Aβ-induced RPE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyloid-beta; SRT1720; Age-related macular degeneration; Barrier integrity; Tight junction; Matrix metalloproteinase-9.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800659

An experimental model of hemolysis-induced acute pancreatitis
Provedor de dados: 56
Autores: Saruc,M.; Yuceyar,H.; Turkel,N.; Ozutemiz,O.; Tuzcuoglu,I.; Yuce,G.; Huseyinov,A.
The literature indicates that acute pancreatitis is a complication of massive hemolysis with a prevalence of about 20%. We describe an experimental model of hemolysis-induced acute pancreatitis. Hemolytic anemia was induced in rats by a single ip injection of 60 mg/kg of 20 mg/ml acetylphenylhydrazine (APH) in 20% (v/v) ethanol on the first experimental day (day 0). One hundred and fifty Wistar albino rats weighing 180-200 g were divided into three groups of 50 animals each: groups 1, 2 and 3 were injected ip with APH, 20% ethanol, and physiological saline, respectively. Ten rats from each group were sacrificed on study days 1, 2, 3, 4 and 5. Serum amylase, lipase levels and pancreatic tissue tumor necrosis factor-alpha (TNF-alpha) and platelet-activating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolysis; Pancreatitis model; Experimental pancreatitis; Etiology; Pathology.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700008

Peripubertal orchidectomy transitorily affects age-associated thymic involution in rats
Provedor de dados: 56
Autores: Pesic,V.; Radojevic,K.; Kosec,D.; Plecas-Solarovic,B.; Perisic,M.; Pilipovic,I.; Leposavic,G.
The role of gonadal hormones in induction and, particularly, maintenance/progression of rat thymic involution, which normally starts around puberty, was reassessed by examining the effects of peripubertal orchidectomy on thymic weight and morphometric parameters at different times up to the age of 10 months. Up to 6 months post-castration both thymic weight and cellularity in orchidectomized (Cx) rats were greater than in age-matched control rats, sham Cx (Sx). The increase in thymic cellularity reflected an increase in thymocyte proliferation rate (the proportion of proliferating cells was 18.6 ± 0.7% in 2-month-old Cx (N = 5) vs 13.4 ± 0.3% (N = 5) in age-matched Sx rats) followed by reduced sensitivity to apoptotic signals (apoptotic thymocytes were 9.8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat thymus; Aging; Orchidectomy; Stereological analysis; Thymocyte proliferation; Thymocyte apoptosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100009

Effects of a PPARG gene variant on obesity characteristics in Brazil
Provedor de dados: 56
Autores: Mattevi,V.S.; Zembrzuski,V.M.; Hutz,M.H.
The contribution of genetic factors to the development of obesity has been widely recognized, but the identity of the genes involved has not yet been fully clarified. Variation in genes involved in adipocyte differentiation and energy metabolism is expected to have a role in the etiology of obesity. We assessed the potential association of a polymorphism in one candidate gene, peroxisome proliferator-activated receptor-gamma (PPARGg), involved in these pathways and obesity-related phenotypes in 335 Brazilians of European descent. All individuals included in the sample were adults. Pregnant women, as well as those individuals with secondary hyperlipidemia due to renal, liver or thyroid disease, and diabetes, were not invited to participate in the study; all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; PPARG gene; Genetic susceptibility; Multifactorial diseases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700006

Circadian time-dependent effects of fencamfamine on inhibition of dopamine uptake and release in rat striatal slices
Provedor de dados: 56
Fencamfamine (FCF) is a central stimulant that facilitates central dopaminergic transmission through inhibition of dopamine uptake and enhanced release of the transmitter. We evaluated the changes in the inhibition of uptake and the release of striatal [3H]-dopamine at 9:00 and 21:00 h, times corresponding to maximal and minimal behavioral responses to FCF, respectively. Adult male Wistar rats (200-250 g) maintained on a 12-h light/12-h dark cycle (lights on at 7:00 h) were used. In the behavioral experiments the rats (N = 8 for each group) received FCF (3.5 mg/kg, ip) or saline at 9:00 or 21:00 h. Fifteen minutes after treatment the duration of activity (sniffing, rearing and locomotion) was recorded for 120 min. The basal motor activity was higher (28.6...
Palavras-chave: Fencamfamine; Chronopharmacology; Behavior; Dopamine uptake; Dopamine release.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500011

Comparison of the effectiveness of initial combined antiretroviral therapy with nelfinavir or efavirenz at a university-based outpatient service in Brazil
Provedor de dados: 56
Autores: Vanni,T.; Morejón,K.M.; Santana,R.C.; Melo,L. de; Ferrão,S.B.R.L.; Amorim,A.P.; Gaspar,G.G.; Ponzi,C.C.; Golin,N.A.; Custódio,F.L.; Marangoni,A.T.D.; Campos Jr.,C.P.; Fonseca,B.A.L.
Since there are some concerns about the effectiveness of highly active antiretroviral therapy in developing countries, we compared the initial combination antiretroviral therapy with zidovudine and lamivudine plus either nelfinavir or efavirenz at a university-based outpatient service in Brazil. This was a retrospective comparative cohort study carried out in a tertiary level hospital. A total of 194 patients receiving either nelfinavir or efavirenz were identified through our electronic database search, but only 126 patients met the inclusion criteria. Patients were included if they were older than 18 years old, naive for antiretroviral therapy, and had at least 1 follow-up visit after starting the antiretroviral regimen. Fifty-one of the included...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIDS; HIV; Antiretroviral treatment; Efavirenz; Nelfinavir; Highly active antiretroviral therapy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700011

Dorsal periaqueductal gray stimulation facilitates anxiety-, but not panic-related, defensive responses in rats tested in the elevated T-maze
Provedor de dados: 56
Autores: Camplesi Jr,M.; Bortoli,V.C.de; Soares,V. de Paula; Nogueira,R.L.; Zangrossi Jr.,H.
The escape response to electrical or chemical stimulation of the dorsal periaqueductal gray matter (DPAG) has been associated with panic attacks. In order to explore the validity of the DPAG stimulation model for the study of panic disorder, we determined if the aversive consequences of the electrical or chemical stimulation of this midbrain area can be detected subsequently in the elevated T-maze. This animal model, derived from the elevated plus-maze, permits the measurement in the same rat of a generalized anxiety- and a panic-related defensive response, i.e., inhibitory avoidance and escape, respectively. Facilitation of inhibitory avoidance, suggesting an anxiogenic effect, was detected in male Wistar rats (200-220 g) tested in the elevated T-maze 30...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panic disorder; Anxiety; Elevated T-maze; Dorsal periaqueductal gray stimulation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100005

Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae)
Provedor de dados: 56
Autores: Silva-Pinhati,A.C.O.; Bacci Jr.,M.; Hinkle,G.; Sogin,M.L.; Pagnocca,F.C.; Martins,V.G.; Bueno,O.C.; Hebling,M.J.A.
Leaf-cutting ants of the genera Atta and Acromyrmex (tribe Attini) are symbiotic with basidiomycete fungi of the genus Leucoagaricus (tribe Leucocoprineae), which they cultivate on vegetable matter inside their nests. We determined the variation of the 28S, 18S, and 5.8S ribosomal DNA (rDNA) gene loci and the rapidly evolving internal transcribed spacers 1 and 2 (ITS1 and ITS2) of 15 sympatric and allopatric fungi associated with colonies of 11 species of leafcutter ants living up to 2,600 km apart in Brazil. We found that the fungal rDNA and ITS sequences from different species of ants were identical (or nearly identical) to each other, whereas 10 GenBank Leucoagaricus species showed higher ITS variation. Our findings suggest that Atta and Acromyrmex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atta; Acromyrmex; Internal transcribed spacer; Leaf-cutting ants; Leucoagaricus; Leucoagaricus gongylophorus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000004

Apolipoprotein CIII polymorphism and triglyceride levels of a Japanese population living in Southern Brazil
Provedor de dados: 56
Autores: Parzianello,L.; Oliveira,G.; Coelho,J.C.
Apolipoprotein CIII (apo-CIII) participates in the regulation of triglyceride-rich lipoprotein metabolism. Several polymorphic sites have been detected within and around the apo-CIII gene. Here, we examined the relationship between apo-CIII SstI polymorphism (CC, CG, GG genotypes) and plasma triglyceride (TG) levels in a group of 159 Japanese individuals living in Southern Brazil. The sample was divided into a group of Japanese descendants (N = 51) with high TG (HTG; &gt;200 mg/dL) and a group of Japanese descendants (N = 108) with normal TG (NTG; &lt;200 mg/dL). TG and total cholesterol levels were analyzed by an enzymatic method using the Labtest-Diagnostic kit and high- and low-density lipoproteins by a direct method using the Labtest-Diagnostic kit and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triglycerides; SstI polymorphism; Apolipoprotein CIII; Japanese population.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600004

PCR-RFLP assay as an option for primary HPV test
Provedor de dados: 56
Autores: Golfetto,L.; Alves,E.V.; Martins,T.R.; Sincero,T.C.M.; Castro,J.B.S.; Dannebrock,C.; Oliveira,J.G.; Levi,J.E.; Onofre,A.S.C.; Bazzo,M.L.
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24.6%) were positive for HPV gene by PCR-Multiplex and were then submitted to PCR-RFLP and PapilloCheck® microarray. There was a genotyping agreement in 71.25% (57/80) on at least one HPV type between PCR-RFLP and PapilloCheck® microarray. In 22 samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; HPV; Screening; PCR-RFLP; PapilloCheck® microarray.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500619

Mechanism of hif-1α mediated hypoxia-induced permeability changes in bladder endothelial cells
Provedor de dados: 56
Autores: Liu,C.; Shui,C.L.; Wang,Q.; Luo,H.; Gu,C.G.
This study aimed to investigate the mechanism of hypoxia-inducible factor-1 alpha (HIF-1α) mediated hypoxia-induced permeability changes in bladder endothelial cells. Models of in vitro hypoxic cell culture of bladder cancer, bladder cancer cells with low HIF-1α expression and HIF-1α RNA interference (RNAi) expression vector were established. Western blot and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of HIF-1α and vascular endothelial growth factor (VEGF) in each group. Bladder cell permeability was determined. Results showed that protein and mRNA expression of HIF-1α and VEGF at 3 and 12 h of hypoxia were significantly higher than normal control (P&lt;0.05), and peaked at 12 h. HIF-1α and VEGF expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bladder cancer; Hypoxia inducible factor-1α; VEGF; Cell permeability; YC-1.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200616

Trypan blue exclusion assay by flow cytometry
Provedor de dados: 56
Autores: Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A.
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307

Role of α2-adrenoceptors in the lateral parabrachial nucleus in the control of body fluid homeostasis
Provedor de dados: 56
Autores: Andrade,C.A.F.; Andrade-Franzé,G.M.F.; De Paula,P.M.; De Luca Jr.,L.A.; Menani,J.V.
Central α2-adrenoceptors and the pontine lateral parabrachial nucleus (LPBN) are involved in the control of sodium and water intake. Bilateral injections of moxonidine (α2-adrenergic/imidazoline receptor agonist) or noradrenaline into the LPBN strongly increases 0.3 M NaCl intake induced by a combined treatment of furosemide plus captopril. Injection of moxonidine into the LPBN also increases hypertonic NaCl and water intake and reduces oxytocin secretion, urinary sodium, and water excreted by cell-dehydrated rats, causing a positive sodium and water balance, which suggests that moxonidine injected into the LPBN deactivates mechanisms that restrain body fluid volume expansion. Pretreatment with specific α2-adrenoceptor antagonists injected into the LPBN...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parabrachial nucleus; Sodium; Natriuresis; Thirst; Hindbrain; Taste.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100011

Effects of lung cancer cell-associated B7-H1 on T-cell proliferation in vitro and in vivo
Provedor de dados: 56
Autores: Chen,K.; Huang,H.T.; Hang,W.J.; Pan,L.B.; Ma,H.T.
B7 homolog 1 (B7-H1) is the most potent immunoinhibitory molecule in the B7 family. In this study, we examined the effects of tumor-associated B7-H1 on T-cell proliferation in lung cancer. The expression of B7-H1 in human adenocarcinoma A549 and mouse Lewis lung carcinoma (LLC) cells were examined by flow cytometry. To assess the in vitro effect of tumor-associated B7-H1 on T-cell proliferation, we isolated T cells from peripheral blood mononuclear cells (PBMCs) of healthy individuals, labeled them with carboxyfluorescein succinimidyl ester, and co-cultured them with A549 cells in the absence or presence of anti-B7-H1 antibody. For in vivo analysis, LLC cells were subcutaneously injected into mice treated or not with anti-B7-H1 antibody. T-cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Costimulatory molecule; B7-H1; A549; Lewis lung carcinoma; Immunosuppression; T-cell proliferation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700701

Effect of the establishment of dominance relationships on cortisol and other metabolic parameters in Nile tilapia (Oreochromis niloticus)
Provedor de dados: 56
Autores: Corrêa,S.A.; Fernandes,M.O.; Iseki,K.K.; Negrão,J.A.
The objective of the present study was to investigate the influence of the establishment of dominance relationships and social stress on plasma cortisol and metabolite levels in Nile tilapia (Oreochromis niloticus). During the 30-day experiment, the fish weighing 236 ± 29 g were kept in individual aquaria, except for two pairings lasting 6 h each. Blood samples were taken from the animals before and after pairing. Display, approach, attack, rebuff, chase flight, and coloration were carried out on days 16 and 30. Activities and behaviors characteristic of the establishment of dominance relationships were described. It was possible to classify all experimental fish (N = 30) as dominant or subordinate. No differences were detected between dominant (N = 15)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tilapia; Social stress; Social relationships; Agonistic behaviors; Cortisol; Dominance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200015

Antagonism of the acute hemodynamic effects of captopril in decompensated congestive heart failure by aspirin administration
Provedor de dados: 56
Autores: Viecili,P.R.; Pamplona,D.; Park,M.; Silva,S.R.; Ramires,J.A.F.; Da Luz,P.L.
The concomitant use of angiotensin-converting enzyme inhibitors and aspirin may cause pharmacological antagonism. Hence we examined the effect of aspirin on the neurohormonal function and hemodynamic response to captopril in heart failure patients. Between April 1999 and August 2000, 40 patients were randomized into four equal groups: 1) captopril, 2) aspirin, 3) captopril-aspirin: captopril was given alone on the first day, followed by aspirin on the remaining days, and 4) aspirin-captopril: aspirin was given alone on the first day, followed by captopril on the remaining days. Hemodynamic, norepinephrine and prostaglandin measurements were performed pre- and post-medication for 4 days. Captopril (50 mg) was given orally every 8 h and 300 mg aspirin was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacological antagonism; Aspirin; Angiotensin-converting enzyme inhibitors; Decompensated heart failure; Hemodynamics and neurohormonal changes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600013

Transplantation and survival of mouse inner ear progenitor/stem cells in the organ of Corti after cochleostomy of hearing-impaired guinea pigs: preliminary results
Provedor de dados: 56
Autores: Barboza Jr.,L.C.M.; Lezirovitz,K.; Zanatta,D.B.; Strauss,B.E.; Mingroni-Netto,R.C.; Oiticica,J.; Haddad,L.A.; Bento,R.F.
In mammals, damage to sensory receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing loss. Here, we investigated whether postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after transplantation into the basal turns of neomycin-injured guinea pig cochleas. We also examined the effects of mIESC transplantation on auditory functions. Eight adult female Cavia porcellus guinea pigs (250-350g) were deafened by intratympanic neomycin delivery. After 7 days, the animals were randomly divided in two groups. The study group (n=4) received transplantation of LacZ-positive mIESCs in culture medium into the scala tympani. The control group (n=4) received culture medium only. At 2 weeks after transplantation, functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cochlea; Hearing loss; Stem cells; Cell transplantation; Ototoxicity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400603

Prevalence and risk factors for HBV, HCV and HDV infections among injecting drug users from Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Oliveira,M.L.A.; Bastos,F.I.; Telles,P.R.; Yoshida,C.F.T.; Schatzmayr,H.G.; Paetzold,U.; Pauli,G.; Schreier,E.
Viral hepatitis constitutes a major health issue, with high prevalence among injecting drug users (IDUs). The present study assessed the prevalence and risk determinants for hepatitis B, C and D viruses (HBV, HCV and HDV) infections among 102 IDUs from Rio de Janeiro, Brazil. Serological markers and HCV-RNA were detected by enzyme immunoassay and nested PCR, respectively. HCV genotyping was determined by restriction fragment length polymorphism analysis (RFLP). HBsAg, anti-HBc and anti-HBs were found in 7.8, 55.8 and 24.7% of IDUs, respectively. In the final logistic regression, HBV infection was independently associated with male homosexual intercourse within the last 5 years (odds ratio (OR) 3.1; 95% confidence interval (CI) 1.1-8.8). No subject...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HBV; HDV; HCV; HCV genotypes; IDU.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900009

Effect of escitalopram on the processing of emotional faces
Provedor de dados: 56
Autores: Alves-Neto,W.C.; Guapo,V.G.; Graeff,F.G.; Deakin,J.F.W.; Del-Ben,C.M.
Serotonin has been implicated in the neurobiology of depressive and anxiety disorders, but little is known about its role in the modulation of basic emotional processing. The aim of this study was to determine the effect of the selective serotonin reuptake inhibitor, escitalopram, on the perception of facial emotional expressions. Twelve healthy male volunteers completed two experimental sessions each, in a randomized, balanced order, double-blind design. A single oral dose of escitalopram (10 mg) or placebo was administered 3 h before the task. Participants were presented to a task composed of six basic emotions (anger, disgust, fear, happiness, sadness, and surprise) that were morphed between neutral and each standard emotion in 10% steps. Escitalopram...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Serotonin; Escitalopram; Facial expression; Emotion; Serotonin reuptake inhibitor.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300010

Hypothyroidism decreases proinsulin gene expression and the attachment of its mRNA and eEF1A protein to the actin cytoskeleton of INS-1E cells
Provedor de dados: 56
Autores: Goulart-Silva,F.; Serrano-Nascimento,C.; Nunes,M.T.
The actions of thyroid hormone (TH) on pancreatic beta cells have not been thoroughly explored, with current knowledge being limited to the modulation of insulin secretion in response to glucose, and beta cell viability by regulation of pro-mitotic and pro-apoptotic factors. Therefore, the effects of TH on proinsulin gene expression are not known. This led us to measure: a) proinsulin mRNA expression, b) proinsulin transcripts and eEF1A protein binding to the actin cytoskeleton, c) actin cytoskeleton arrangement, and d) proinsulin mRNA poly(A) tail length modulation in INS-1E cells cultured in different media containing: i) normal fetal bovine serum - FBS (control); ii) normal FBS plus 1 µM or 10 nM T3, for 12 h, and iii) FBS depleted of TH for 24 h (Tx)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proinsulin mRNA; EEF1A protein; Cytoskeleton; Hypothyroidism; INS-1E cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000012

Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum
Provedor de dados: 56
Autores: Noël,F.; Geurts,M.; Maloteaux,J.-M.
Dopamine nigrostriatal neurons are important for motor control and may contain a particularly dense population of ryanodine receptors involved in the control of dopamine release. To test this hypothesis, we used a classical model of unilateral selective lesion of these neurons in rats based on 6-hydroxydopamine (6-OHDA) injection into the substantia nigra. Binding of [3H]-GBR 12935, used as a presynaptic marker since it labels specifically the dopamine uptake complex, was dramatically decreased by 83-100% in striatum homogenates after 6-OHDA lesion. On the contrary, no reduction of [3H]-ryanodine binding was observed. The present data indicate that [3H]-ryanodine binding sites present in rat striatum are not preferentially localized in dopaminergic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ryanodine; Striatum; 6-hydroxydopamine; Dopamine neurons; Neuroleptic malignant syndrome.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200013

Isolation and culture of umbilical vein mesenchymal stem cells
Provedor de dados: 56
Autores: Covas,D.T.; Siufi,J.L.C.; Silva,A.R.L.; Orellana,M.D.
Bone marrow contains a population of stem cells that can support hematopoiesis and can differentiate into different cell lines including adipocytes, osteocytes, chondrocytes, myocytes, astrocytes, and tenocytes. These cells have been denoted mesenchymal stem cells. In the present study we isolated a cell population derived from the endothelium and subendothelium of the umbilical cord vein which possesses morphological, immunophenotypical and cell differentiation characteristics similar to those of mesenchymal stem cells isolated from bone marrow. The cells were isolated from three umbilical cords after treatment of the umbilical vein lumen with collagenase. The cell population isolated consisted of adherent cells with fibroblastoid morphology which, when...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mesenchymal stem cells; Umbilical vein; Adipocytes; Osteocytes; Cell differentiation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900006

Screening of antibacterial extracts from plants native to the Brazilian Amazon Rain Forest and Atlantic Forest
Provedor de dados: 56
Autores: Suffredini,I.B.; Sader,H.S.; Gonçalves,A.G.; Reis,A.O.; Gales,A.C.; Varella,A.D.; Younes,R.N.
More than 20% of the world's biodiversity is located in Brazilian forests and only a few plant extracts have been evaluated for potential antibacterial activity. In the present study, 705 organic and aqueous extracts of plants obtained from different Amazon Rain Forest and Atlantic Forest plants were screened for antibacterial activity at 100 µg/ml, using a microdilution broth assay against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli. One extract, VO581, was active against S. aureus (minimum inhibitory concentration (MIC) = 140 µg/ml and minimal bactericidal concentration (MBC) = 160 µg/ml, organic extract obtained from stems) and two extracts were active against E. faecalis, SM053 (MIC = 80 µg/ml and MBC = 90...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant extract; Antibacterial activity; Screening; Amazon Rain Forest; Atlantic Forest.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300015

The blockade of cyclooxygenases-1 and -2 reduces the effects of hypoxia on endothelial cells
Provedor de dados: 56
Autores: Gloria,M.A.; Cenedeze,M.A.; Pacheco-Silva,A.; Câmara,N.O.S.
Hypoxia activates endothelial cells by the action of reactive oxygen species generated in part by cyclooxygenases (COX) production enhancing leukocyte transmigration. We investigated the effect of specific COX inhibition on the function of endothelial cells exposed to hypoxia. Mouse immortalized endothelial cells were subjected to 30 min of oxygen deprivation by gas exchange. Acridine orange/ethidium bromide dyes and lactate dehydrogenase activity were used to monitor cell viability. The mRNA of COX-1 and -2 was amplified and semi-quantified before and after hypoxia in cells treated or not with indomethacin, a non-selective COX inhibitor. Expression of RANTES (regulated upon activation, normal T cell expressed and secreted) protein and the protective role...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelial cell; Hypoxia; Indomethacin; Cyclooxygenase; Heme oxygenase 1.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900006

Galactosaminoglycans from normal myometrium and leiomyoma
Provedor de dados: 56
Autores: Berto,A.G.A.; Oba,S.M.; Michelacci,Y.M.; Sampaio,L.O.
In many tumors, the amount of chondroitin sulfate in the extracellular matrix has been shown to be elevated when compared to the corresponding normal tissue. Nevertheless, the degree of chondroitin sulfate increase varies widely. In order to investigate a possible correlation between the amount of chondroitin sulfate and tumor size, several individual specimens of human leiomyoma, a benign uterine tumor, were analyzed. The glycosaminoglycans from eight tumors were extracted and compared with those from the respective adjacent normal myometrium. The main glycosaminoglycan found in normal myometrium was dermatan sulfate, with small amounts of chondroitin sulfate and heparan sulfate. In leiomyoma, both dermatan sulfate and chondroitin sulfate were detected...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Galactosaminoglycan; Glycosaminoglycan; Chondroitin sulfate; Dermatan sulfate; Leiomyoma; Myometrium.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500011

The efficacy of moxifloxacin-based triple therapy in treatment of Helicobacter pylori infection: a systematic review and meta-analysis of randomized clinical trials
Provedor de dados: 56
Autores: Zhang,G.; Zou,J.; Liu,F.; Bao,Z.; Dong,F.; Huang,Y.; Yin,S.
Recent evidence shows that moxifloxacin could exert an antimicrobial effect against Helicobacter pylori in both in vitroand in vivo models. To systematically evaluate whether moxifloxacin-containing triple therapy could improve eradication rates and reduce side effects in first-line or second-line anti-H. pyloritreatment, eligible articles were identified by searches of electronic databases. We included all randomized trials comparing moxifloxacin-based triple therapy with standard triple or quadruple therapy during H. pylori eradication treatment. Statistical analysis was performed with Review Manager 5.0.10. Subanalysis/sensitivity analysis was also performed. We identified seven randomized trials (n=1263). Pooled H. pylori eradication rates were 79.03%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moxifloxacin; Helicobacter pylori; Eradication rate; Side effects; Meta-analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700607

Fibrinolytic action on fresh human clots of whole body extracts and two semipurified fractions from Lonomia achelous caterpillar
Provedor de dados: 56
Autores: Coll-Sangrona,E.; Arocha-Piñango,C.L.
The severe bleeding diathesis produced by intoxication with the venom of Lonomia achelous caterpillars is characterized by prolonged bleeding from superficial skin wounds as well as massive hemorrhage into body cavities. The aim of the present study was to evaluate the effect of the crude venom and its fibrinolytic fractions on in vitro lysis of whole blood clots. Venom fractions with fibrinolytic activity were obtained by gel filtration chromatography on Sephadex G75 using imidazole buffer, pH 7.4, at a flow rate of 24 ml/h. Four peaks with fibrinolytic activity were obtained by this method. The highest activity was found in the first two peaks (both peaks were used for the experiments). The results show that the caterpillar venom degraded the preformed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lonomia achelous caterpillars; Fibrinolysis; Clot lysis; Plasmin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600009

Exploration of gene functions for esophageal squamous cell carcinoma using network-based guilt by association principle
Provedor de dados: 56
Autores: Wu,Wei; Huang,Bo; Yan,Yan; Zhong,Zhi-Qiang.
Gene networks have been broadly used to predict gene functions based on guilt by association (GBA) principle. Thus, in order to better understand the molecular mechanisms of esophageal squamous cell carcinoma (ESCC), our study was designed to use a network-based GBA method to identify the optimal gene functions for ESCC. To identify genomic bio-signatures for ESCC, microarray data of GSE20347 were first downloaded from a public functional genomics data repository of Gene Expression Omnibus database. Then, differentially expressed genes (DEGs) between ESCC patients and controls were identified using the LIMMA method. Afterwards, construction of differential co-expression network (DCN) was performed relying on DEGs, followed by gene ontology (GO) enrichment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal squamous cell carcinoma; Gene oncology; Guilt by association; Differentially expressed genes; Area under the curve.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600601

Nuclear calcium signaling: a cell within a cell
Provedor de dados: 56
Autores: Rodrigues,M.A.; Gomes,D.A.; Nathanson,M.H.; Leite,M.F.
Calcium (Ca2+) is a versatile second messenger that regulates a wide range of cellular functions. Although it is not established how a single second messenger coordinates diverse effects within a cell, there is increasing evidence that the spatial patterns of Ca2+ signals may determine their specificity. Ca2+ signaling patterns can vary in different regions of the cell and Ca2+ signals in nuclear and cytoplasmic compartments have been reported to occur independently. No general paradigm has been established yet to explain whether, how, or when Ca2+ signals are initiated within the nucleus or their function. Here we highlight that receptor tyrosine kinases rapidly translocate to the nucleus. Ca2+ signals that are induced by growth factors result from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Proliferation; Receptor tyrosine kinases; Nucleus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100004

Lack of evidence for regulation of cardiac P-type ATPases and MAP kinases in transgenic mice with cardiac-specific overexpression of constitutively active α1B-adrenoceptors
Provedor de dados: 56
Autores: Barreto,F.; Rezende,D.C.; Scaramello,C.B.V.; Silva,C.L.M.; Cunha,V.M.N.; Caricati-Neto,A.; Jurkiewicz,A.; Noël,F.; Quintas,L.E.M.
The regulatory function of α1B-adrenoceptors in mammalian heart homeostasis is controversial. The objective of the present study was to characterize the expression/activity of key proteins implicated in cardiac calcium handling (Na+/K+-ATPase and Ca2+-ATPases) and growth (ERK1/2, JNK1/2 and p38) in mice with cardiac-selective overexpression of constitutively active mutant α1B-adrenoceptor (CAMα1B-AR), which present a mild cardiac hypertrophy phenotype. Immunoblot assays showed that myocardial plasma membrane Ca2+-ATPase (PMCA) expression was increased by 30% in CAMα1B-AR mice (N = 6, P &lt; 0.05), although there was no change in sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2) expression. Moreover, total Ca2+-ATPase activity was not modified, but a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α1B-adrenoceptors; Ca2+-ATPases; Cardiac hypertrophy; Na+/K+-ATPase; Mitogen-activated protein kinases.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500012

Stabilization of partially folded states in protein folding/misfolding transitions by hydrostatic pressure
Provedor de dados: 56
Autores: Ferreira,S.T.; Chapeaurouge,A.; De Felice,F.G.
In the last few years, hydrostatic pressure has been extensively used in the study of both protein folding and misfolding/aggregation. Compared to other chemical or physical denaturing agents, a unique feature of pressure is its ability to induce subtle changes in protein conformation, which allow the stabilization of partially folded intermediate states that are usually not significantly populated under more drastic conditions (e.g., in the presence of chemical denaturants or at high temperatures). Much of the recent research in the field of protein folding has focused on the characterization of folding intermediates since these species appear to be involved in a variety of disease-causing protein misfolding and aggregation events. The exact mechanisms of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High pressure; Protein folding; Misfolding; Amyloid; Aggregation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800009

Effect of 17ß-estradiol or alendronate on the bone densitometry, bone histomorphometry and bone metabolism of ovariectomized rats
Provedor de dados: 56
Autores: da Paz,L.H.B.C.; de Falco,V.; Teng,N.C.; dos Reis,L.M.; Pereira,R.M.R.; Jorgetti,V.
The objective of the present study was to evaluate the effect of 17ß-estradiol or alendronate in preventing bone loss in 3-month-old ovariectomized Wistar rats. One group underwent sham ovariectomy (control, N = 10), and the remaining three underwent double ovariectomy. One ovariectomized group did not receive any treatment (OVX, N = 12). A second received subcutaneous 17ß-estradiol at a dose of 30 µg/kg for 6 weeks (OVX-E, N = 11) and a third, subcutaneous alendronate at a dose of 0.1 mg/kg for 6 weeks (OVX-A, N = 8). Histomorphometry, densitometry, osteocalcin and deoxypyridinoline measurements were applied to all groups. After 6 weeks there was a significant decrease in bone mineral density (BMD) at the trabecular site (distal femur) in OVX rats. Both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat; 17ß-estradiol; Alendronate; Densitometry; Histomorphometry.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800007

Association of NOS3 gene variants and clinical contributors of hypoxic-ischemic encephalopathy
Provedor de dados: 56
Autores: Kuzmanić Šamija,R.; Primorac,D.; Rešić,B.; Pavlov,V.; Čapkun,V.; Punda,H.; Lozić,B.; Zemunik,T.
The aim of this study was to analyze the association of different clinical contributors of hypoxic-ischemic encephalopathy with NOS3 gene polymorphisms. A total of 110 children with hypoxic-ischemic encephalopathy and 128 control children were selected for this study. Association of gender, gestational age, birth weight, Apgar score, cranial ultrasonography, and magnetic resonance imaging findings with genotypic data of six haplotype-tagging single nucleotide polymorphisms and the most commonly investigated rs1800779 and rs2070744 polymorphisms was analyzed. The TGT haplotype of rs1800783, rs1800779, and rs2070744 polymorphisms was associated with hypoxic-ischemic encephalopathy. Children with the TGT haplotype were infants below 32 weeks of gestation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinical contributors; Hypoxic-ischemic encephalopathy; NOS3 gene polymorphisms.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000869

Mutations in the SRY, DAX1, SF1 and WNT4 genes in Brazilian sex-reversed patients
Provedor de dados: 56
Autores: Domenice,S.; Corrêa,R.V.; Costa,E.M.F.; Nishi,M.Y.; Vilain,E.; Arnhold,I.J.P.; Mendonca,B.B.
In most mammals, male development is triggered by the transient expression of the SRY gene, which initiates a cascade of gene interactions ultimately leading to the formation of a testis from the indifferent fetal gonad. Mutation studies have identified several genes essential for early gonadal development. We report here a molecular study of the SRY, DAX1, SF1 and WNT4 genes, mainly involved in sexual determination, in Brazilian 46,XX and 46,XY sex-reversed patients. The group of 46,XX sex-reversed patients consisted of thirteen 46,XX true hermaphrodites and four 46,XX males, and was examined for the presence of the SRY gene and for the loss of function (inactivating mutations and deletions) of DAX1 and WNT4 genes. In the second group consisting of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex reversal; SRY gene; DAX1 gene; SF1 gene; WNT4 gene.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100020

Correlations between pulse oximetry and peak expiratory flow in acute asthma
Provedor de dados: 56
Autores: Andrade,C. Ribeiro de; Duarte,M.C.; Camargos,P.
Few studies are available concerning correlations between pulse oximetry and peak expiratory flow in children and adolescents with acute asthma. Although the Global Initiative for Asthma states that measurements of lung function and oximetry are critical for the assessment of patients, it is not clear if both methods should necessarily be included in their evaluation. Since there is a significant difference in cost between pulse oximetry equipment and peak expiratory flow devices, we determined whether clinical findings and peak expiratory flow measurements are sufficient to determine the severity of acute asthma. The present prospective observational study was carried out to determine if there is correlation between pulse oximetry and peak expiratory flow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute asthma; Pulse oximetry; Peak expiratory flow.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400006

Aluminum induces lipid peroxidation and aggregation of human blood platelets
Provedor de dados: 56
Aluminum (Al3+) intoxication is thought to play a major role in the development of Alzheimer's disease and in certain pathologic manifestations arising from long-term hemodialysis. Although the metal does not present redox capacity, it can stimulate tissue lipid peroxidation in animal models. Furthermore, in vitro studies have revealed that the fluoroaluminate complex induces diacylglycerol formation, 43-kDa protein phosphorylation and aggregation. Based on these observations, we postulated that Al3+-induced blood platelet aggregation was mediated by lipid peroxidation. Using chemiluminescence (CL) of luminol as an index of total lipid peroxidation capacity, we established a correlation between lipid peroxidation capacity and platelet aggregation. Al3+...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aluminum; Lipid peroxidation; Chemiluminescence; Platelet aggregation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500005

Enhanced expression of Ang-(1-7) during pregnancy
Provedor de dados: 56
Autores: Brosnihan,K.B.; Neves,L.A.A.; Anton,L.; Joyner,J.; Valdes,G.; Merrill,D.C.
Pregnancy is a physiological condition characterized by a progressive increase of the different components of the renin-angiotensin system (RAS). The physiological consequences of the stimulated RAS in normal pregnancy are incompletely understood, and even less understood is the question of how this system may be altered and contribute to the hypertensive disorders of pregnancy. Findings from our group have provided novel insights into how the RAS may contribute to the physiological condition of pregnancy by showing that pregnancy increases the expression of both the vasodilator heptapeptide of the RAS, angiotensin-(1-7) [Ang-(1-7)], and of a newly cloned angiotensin converting enzyme (ACE) homolog, ACE2, that shows high catalytic efficiency for Ang II...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pregnancy; Renin-angiotensin system; Preeclampsia; Angiotensin-(1-7).
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800017

Radiography, computed tomography and magnetic resonance imaging at 0.5 Tesla of mechanically inducedosteoarthritis in rabbit knees
Provedor de dados: 56
Autores: Torelli,S.R.; Rahal,S.C.; Volpi,R.S.; Yamashita,S.; Mamprim,M.J.; Crocci,A.J.
In the present experimental study we assessed induced osteoarthritis data in rabbits, compared three diagnostic methods, i.e., radiography (XR), computed tomography (CT) and magnetic resonance imaging (MRI), and correlated the imaging findings with those obtained by macroscopic evaluation. Ten young female rabbits of the Norfolk breed were used. Seven rabbits had the right knee immobilized in extension for a period of 12 weeks (immobilized group), and three others did not have a limb immobilized and were maintained under the same conditions (control group). Alterations observed by XR, CT and MRI after the period of immobilization were osteophytes, osteochondral lesions, increase and decrease of joint space, all of them present both in the immobilized and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoarthritis; Rabbit knee osteoarthritis; Imaging diagnosis; Knee immobilization; Radiography; Computed tomography; Magnetic resonance imaging.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400006

Effect of melatonin administration on subjective sleep quality in chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Nunes,D.M.; Mota,R.M.S.; Machado,M.O.; Pereira,E.D.B.; de Bruin,V.M.S.; de Bruin,P.F.C.
Disturbed sleep is common in chronic obstructive pulmonary disease (COPD). Conventional hypnotics worsen nocturnal hypoxemia and, in severe cases, can lead to respiratory failure. Exogenous melatonin has somnogenic properties in normal subjects and can improve sleep in several clinical conditions. This randomized, double-blind, placebo-controlled study was carried out to determine the effects of melatonin on sleep in COPD. Thirty consecutive patients with moderate to very severe COPD were initially recruited for the study. None of the participants had a history of disease exacerbation 4 weeks prior to the study, obstructive sleep apnea, mental disorders, current use of oral steroids, methylxanthines or hypnotic-sedative medication, nocturnal oxygen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Melatonin; Sleep.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000016

Sleep pattern and learning in knockdown mice with reduced cholinergic neurotransmission
Provedor de dados: 56
Autores: Queiroz,C.M.; Tiba,P.A.; Moreira,K.M.; Guidine,P.A.M.; Rezende,G.H.S.; Moraes,M.F.D.; Prado,M.A.M.; Prado,V.F.; Tufik,S.; Mello,L.E.
Impaired cholinergic neurotransmission can affect memory formation and influence sleep-wake cycles (SWC). In the present study, we describe the SWC in mice with a deficient vesicular acetylcholine transporter (VAChT) system, previously characterized as presenting reduced acetylcholine release and cognitive and behavioral dysfunctions. Continuous, chronic ECoG and EMG recordings were used to evaluate the SWC pattern during light and dark phases in VAChT knockdown heterozygous (VAChT-KDHET, n=7) and wild-type (WT, n=7) mice. SWC were evaluated for sleep efficiency, total amount and mean duration of slow-wave, intermediate and paradoxical sleep, as well as the number of awakenings from sleep. After recording SWC, contextual fear-conditioning tests were used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep-wake cycle; Intermediate sleep; Acetylcholine; Contextual fear conditioning; Memory; Neurodegenerative disorders.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000844

Effect of Sapindus trifoliatus on hyperalgesic in vivo migraine models
Provedor de dados: 56
Autores: Arulmozhi,D.K.; Veeranjaneyulu,A.; Bodhankar,S.L.; Arora,S.K.
Phytotherapies have offered alternative sources of therapy for migraine and gained much importance in prophylactic treatment. Sapindus trifoliatus is a medium-sized deciduous tree growing wild in south India that belongs to the family Sapindaceae. The pericarp is reported for various medicinal properties. A thick aqueous solution of the pericarp is used for the treatment of hemicrania, hysteria or epilepsy in folklore medicine. We have investigated the antihyperalgesic effects of the lyophilized aqueous extract of S. trifoliatus in animal models predictive of experimental migraine models using morphine withdrawal-induced hyperalgesia on the hot-plate test and on 0.3% acetic acid-induced abdominal constrictions in adult male Swiss albino mice. The extract...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sapindus trifoliatus; Hyperalgesia; Migraine; Dopamine; D2 receptor; Morphine withdrawal.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300019

Rapid test for the evaluation of the activity of the prodrug hydroxymethylnitrofurazone in the processing of Trypanosoma cruzi messenger RNAs
Provedor de dados: 56
Autores: Barbosa,C.F.; Okuda,E.S.; Chung,M.C.; Ferreira,E.I.; Cicarelli,R.M.B.
No fully effective treatment has been developed since the discovery of Chagas' disease by Carlos Chagas in 1909. Since drug-resistant Trypanosoma cruzi strains are occurring and the current therapy is effectiveness in the acute phase but with various adverse side effects, more studies are needed to characterize the susceptibility of T. cruzi to new drugs. Many natural and/or synthetic substances showing trypanocidal activity have been used, even though they are not likely to be turned into clinically approved drugs. Originally, drug screening was performed using natural products, with only limited knowledge of the molecular mechanism involved in the development of diseases. Trans-splicing, which is unusual RNA processing reaction and occurs in nematodes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Trans-splicing; Trypanocidal drugs; Permeable cells; RNA processing; Hydroxymethylnitrofurazone.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100005

Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity
Provedor de dados: 56
Autores: Bianchini,A.E.; Garlet,Q.I.; da Cunha,J.A.; Bandeira Junior,G.; Brusque,I.C.M.; Salbego,J.; Heinzmann,B.M.; Baldisserotto,B.
This study evaluated the anesthetic potential of thymol and carvacrol, and their influence on acetylcholinesterase (AChE) activity in the muscle and brain of silver catfish (Rhamdia quelen). The AChE activity of S-(+)-linalool was also evaluated. We subsequently assessed the effects of thymol and S-(+)-linalool on the GABAergic system. Fish were exposed to thymol and carvacrol (25, 50, 75, and 100 mg/L) to evaluate time for anesthesia and recovery. Both compounds induced sedation at 25 mg/L and anesthesia with 50–100 mg/L. However, fish exposed to carvacrol presented strong muscle contractions and mortality. AChE activity was increased in the brain of fish at 50 mg/L carvacrol and 100 mg/L thymol, and decreased in the muscle at 100 mg/L carvacrol....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AChE; Anesthesia; Benzodiazepine; Fish; GABAA; Terpenoids.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200610

CTLA4 enhances the osteogenic differentiation of allogeneic human mesenchymal stem cells in a model of immune activation
Provedor de dados: 56
Autores: Dai,F.; Zhang,F.; Sun,D.; Zhang,Z.H.; Dong,S.W.; Xu,J.Z.
Allogeneic mesenchymal stem cells (allo-MSCs) have recently garnered increasing interest for their broad clinical therapy applications. Despite this, many studies have shown that allo-MSCs are associated with a high rate of graft rejection unless immunosuppressive therapy is administered to control allo-immune responses. Cytotoxic T-lymphocyte-associated protein 4 (CTLA4) is a co-inhibitory molecule expressed on T cells that mediates the inhibition of T-cell function. Here, we investigated the osteogenic differentiation potency of allo-MSCs in an activated immune system that mimics the in vivo allo-MSC grafting microenvironment and explored the immunomodulatory role of the helper T cell receptor CTLA4 in this process. We found that MSC osteogenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CTLA4; Mesenchymal stem cells; Immunomodulatory; Bone tissue engineering.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700629

Retrospective analysis of efficacy, safety, and prognostic factors in a cohort of Chinese hepatocellular carcinoma patients treated with drug-eluting bead transarterial chemoembolization
Provedor de dados: 56
Autores: Chen,Sihan; Ji,Rengbin; Shi,Xiaojun; Wang,Zhe; Zhu,Dedong.
The aim of our study was to assess the efficacy, safety, and prognostic factors of drug-eluting bead transarterial chemoembolization (DEB-TACE) in Chinese hepatocellular carcinoma (HCC) patients. Patients (n=102) diagnosed as primary HCC were consecutively enrolled in this retrospective cohort study. Treatment responses were assessed following the modified Response Evaluation Criteria in Solid Tumors. Progression-free survival (PFS) and overall survival (OS) were evaluated, and adverse events (AEs) as well as liver function-related laboratory indexes of all DEB-TACE records (N=131) were assessed. Complete response (CR) rate, objective response rate, and disease control rate were 51.0, 87.3, and 95.1%, respectively, at 1–3 months post DEB-TACE. The mean PFS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Drug-eluting bead transarterial chemoembolization; China; Treatment response; Survival; Adverse events.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200608

Inflammatory biomarkers responses after acute whole body vibration in fibromyalgia
Provedor de dados: 56
Autores: Ribeiro,V.G.C.; Mendonça,V.A.; Souza,A.L.C.; Fonseca,S.F.; Camargos,A.C.R.; Lage,V.K.S.; Neves,C.D.C.; Santos,J.M.; Teixeira,L.A.C.; Vieira,E.L.M.; Teixeira Junior,A.L.; Mezêncio,B.; Fernandes,J.S.C.; Leite,H.R.; Poortmans,J.R.; Lacerda,A.C.R.
The aims of this study were 1) to characterize the intensity of the vibration stimulation in women diagnosed with fibromyalgia (FM) compared to a control group of healthy women (HW) matched by age and anthropometric parameters, and 2) to investigate the effect of a single session of whole body vibration (WBV) on inflammatory responses. Levels of adipokines, soluble tumor necrosis factor receptors (sTNFr1, sTNFr2), and brain-derived neurotrophic factor (BDNF) were determined by enzyme-linked immunosorbent assay. Oxygen consumption (VO2) was estimated by a portable gas analysis system, heart rate (HR) was measured using a HR monitor, and perceived exertion (RPE) was evaluated using the Borg scale of perceived exertion. Acutely mild WBV increased VO2 and HR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Fibromyalgia; Clinical trial; Inflammation; Acute exercise.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400615

Expression of c-erbB-2, p53 and c-myc proteins in male breast carcinoma: Comparison with traditional prognostic factors and survival
Provedor de dados: 56
Autores: Mourão Netto,M.; Logullo,A.F.; Nonogaki,S.; Brentani,R.R.; Brentani,M.M.
There are few data evaluating biological markers for men with breast cancer. The purpose of the present study was to analyze the expression of the oncogenes c-erbB-2 and c-myc and of the suppressor gene p53 by immunohistochemical techniques in archival paraffin-embedded tissue blocks of 48 male breast cancer patients, treated at the A.C. Camargo Cancer Hospital, São Paulo, SP, Brazil. The results were compared with clinicopathological prognostic features. Immunopositivity of c-erbB-2, p53 and c-myc was detected in 62.5, 16.7 and 20.8% of the cases analyzed, respectively. Estrogen and progesterone receptors were positive in 75 and 69% of the cases, respectively. Increasing staging was statistically associated with c-erbB-2 (P = 0.04) and weakly related to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P53; C-myc; C-erbB-2; Male breast cancer.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700008

Differential binding with ERα and ERβ of the phytoestrogen-rich plant Pueraria mirifica
Provedor de dados: 56
Autores: Boonchird,C.; Mahapanichkul,T.; Cherdshewasart,W.
Variations in the estrogenic activity of the phytoestrogen-rich plant, Pueraria mirifica, were determined with yeast estrogen screen (YES) consisting of human estrogen receptors (hER) hERα and hERβ and human transcriptional intermediary factor 2 (hTIF2) or human steroid receptor coactivator 1 (hSRC1), respectively, together with the β-galactosidase expression cassette. Relative estrogenic potency was expressed by determining the β-galactosidase activity (EC50) of the tuber extracts in relation to 17β-estradiol. Twenty-four and 22 of the plant tuber ethanolic extracts interacted with hERα and hERβ, respectively, with a higher relative estrogenic potency with hERβ than with hERα. Antiestrogenic activity of the plant extracts was also determined by incubation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YES assay; Estrogen receptor α (ERα); Estrogen receptor β (ERβ); Phytoestrogen; Isoflavonoid; Pueraria mirifica.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200010

Autonomic processing of the cardiovascular reflexes in the nucleus tractus solitarii
Provedor de dados: 56
The nucleus tractus solitarii (NTS) receives afferent projections from the arterial baroreceptors, carotid chemoreceptors and cardiopulmonary receptors and as a function of this information produces autonomic adjustments in order to maintain arterial blood pressure within a narrow range of variation. The activation of each of these cardiovascular afferents produces a specific autonomic response by the excitation of neuronal projections from the NTS to the ventrolateral areas of the medulla (nucleus ambiguus, caudal and rostral ventrolateral medulla). The neurotransmitters at the NTS level as well as the excitatory amino acid (EAA) receptors involved in the processing of the autonomic responses in the NTS, although extensively studied, remain to be...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: L-glutamate; Excitatory amino acid receptors; NMDA receptors; Baroreflex; Chemoreflex; Bezold-Jarisch reflex; AP-5; Kynureic acid.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400015

P-selectin, carcinoma metastasis and heparin: novel mechanistic connections with therapeutic implications
Provedor de dados: 56
Autores: Varki,A.; Varki,N.M.
Metastasis is a multistep cascade initiated when malignant cells penetrate the tissue surrounding the primary tumor and enter the bloodstream. Classic studies indicated that blood platelets form complexes around tumor cells in the circulation and facilitate metastases. In other work, the anticoagulant drug heparin diminished metastasis in murine models, as well is in preliminary human studies. However, attempts to follow up the latter observation using vitamin K antagonists failed, indicating that the primary mechanism of heparin action was unrelated to its anticoagulant properties. Other studies showed that the overexpression of sialylated fucosylated glycans in human carcinomas is associated with a poor prognosis. We have now brought all these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparin; Carcinomas; P-selectin; Mucin; Metastasis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600003

Central release of nitric oxide mediates antinociception induced by aerobic exercise
Provedor de dados: 56
Autores: Galdino,G.S.; Duarte,I.D.; Perez,A.C.
Nitric oxide (NO) is a soluble gas that participates in important functions of the central nervous system, such as cognitive function, maintenance of synaptic plasticity for the control of sleep, appetite, body temperature, neurosecretion, and antinociception. Furthermore, during exercise large amounts of NO are released that contribute to maintaining body homeostasis. Besides NO production, physical exercise has been shown to induce antinociception. Thus, the present study aimed to investigate the central involvement of NO in exercise-induced antinociception. In both mechanical and thermal nociceptive tests, central [intrathecal (it) and intracerebroventricular (icv)] pretreatment with inhibitors of the NO/cGMP/KATP pathway (L-NOArg, ODQ, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Exercise; Pain; Antinociception.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900790

HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
Provedor de dados: 56
Autores: Rossini,M.A.A.; Diaz,R.S.; Caseiro,M.; Turcato,G.; Accetturi,C.A.; Sabino,E.C.
In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV-1 subtypes; Intravenous drug users; Molecular epidemiology; Heteroduplex mobility analysis; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100005

Respiratory panic disorder subtype and sensitivity to the carbon dioxide challenge test
Provedor de dados: 56
Autores: Valença,A.M.; Nardi,A.E.; Nascimento,I.; Zin,W.A.; Versiani,M.
The aim of the present study was to verify the sensitivity to the carbon dioxide (CO2) challenge test of panic disorder (PD) patients with respiratory and nonrespiratory subtypes of the disorder. Our hypothesis is that the respiratory subtype is more sensitive to 35% CO2. Twenty-seven PD subjects with or without agoraphobia were classified into respiratory and nonrespiratory subtypes on the basis of the presence of respiratory symptoms during their panic attacks. The tests were carried out in a double-blind manner using two mixtures: 1) 35% CO2 and 65% O2, and 2) 100% atmospheric compressed air, 20 min apart. The tests were repeated after 2 weeks during which the participants in the study did not receive any psychotropic drugs. At least 15 of 16 (93.7%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CO2; Anxiety; Breathing; Respiration.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700004

The availability of full match sibling donors and feasibility of allogeneic bone marrow transplantation in Brazil
Provedor de dados: 56
Autores: Eid,K.A.B.; Miranda,E.C.M.; Vigorito,A.C.; Aranha,F.J.P.; Oliveira,G.B.; De Souza,C.A.
The feasibility of allogeneic bone marrow transplantation (alloBMT) in a developing country has not yet been demonstrated. Many adverse factors including social and economic limitations may reduce the overall results of this complex and expensive procedure. Our objective was to characterize the most important clinical, social and economic features of candidates for transplantation and their potential donors as well as the influence of these factors on overall survival in a retrospective and exploratory analysis at a university hospital. From July 1993 to July 2001, candidates for BMT were referred to the Bone Marrow Transplantation Unit by Hematology and Oncology Centers from several regions of Brazil. A total of 1138 patients were referred to us as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allogeneic bone marrow transplantation; Selection of donors; Social and economic conditions.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300005

Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes
Provedor de dados: 56
Autores: Aires,M.B.; Santos,J.R.A.; Souza,K.S.; Farias,P.S.; Santos,A.C.V.; Fioretto,E.T.; Maria,D.A.
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral yolk sac; Diabetes; Embryo; Pregnancy; Cell markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800676

Long-term outcome of 25 children and adolescents with severe aplastic anemia treated with antithymocyte globulin
Provedor de dados: 56
Autores: de-Medeiros,C.R.; Ribeiro,R.C.; Bittencourt,M.A.; Zanis-Neto,J.; Pasquini,R.
Severe aplastic anemia (SAA) is probably an immune-mediated disorder, and immunosuppressive therapy is recommended for patients with no available donor for bone marrow transplant. Between October 1984 and November 1987, 25 consecutive children and adolescents with SAA with no HLA-compatible marrow donor received equine antithymocyte globulin (ATG) (15 mg kg-1 day-1) for 10 days. The patients were evaluated 6 weeks, 6 months, and 12 months after starting ATG treatment. Thereafter, patients were evaluated yearly until July 1998. Median age was 10 years (range, 1.5-20 years), granulocyte counts on referral ranged from 0.032 to 1.4 x 10(9)/l (median 0.256 x 10(9)/l), and 12 patients had granulocyte counts &amp;lt;0.2 x 10(9)/l. At a median follow-up of 9.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aplastic anemia; Children; Adolescents; Antithymocyte globulin.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500010

Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system
Provedor de dados: 56
Autores: Fernandes,A.M.; Marinho,P.A.N.; Sartore,R.C.; Paulsen,B.S.; Mariante,R.M.; Castilho,L.R.; Rehen,S.K.
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up hES cell production. Maintenance of pluripotency in the microcarrier-based stirred system was shown by immunocytochemical and flow cytometry analyses for pluripotency-associated markers. The formation of cavitated embryoid bodies expressing markers of endoderm, ectoderm and mesoderm was further evidence of maintenance of differentiation capability. Cell yield per volume of medium spent was more than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human embryonic stem cells; Scale-up; Microcarriers; Spinner bioreactors.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600007

Methylmercury inhibits prolactin release in a cell line of pituitary origin
Provedor de dados: 56
Autores: Maués,L.A.L.; Macchi,B.M.; Crespo-López,M.E.; Nasciutti,L.E.; Picanço-Diniz,D.L.W.; Antunes-Rodrigues,J.; Nascimento,J.L.M. do.
Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Methylmercury; Prolactin; Oxidative stress; Pituitary; Nitric oxide.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800691

Thyroid peroxidase activity is inhibited by amino acids
Provedor de dados: 56
Autores: Carvalho,D.P.; Ferreira,A.C.F.; Coelho,S.M.; Moraes,J.M.; Camacho,M.A.S.; Rosenthal,D.
Normal in vitro thyroid peroxidase (TPO) iodide oxidation activity was completely inhibited by a hydrolyzed TPO preparation (0.15 mg/ml) or hydrolyzed bovine serum albumin (BSA, 0.2 mg/ml). A pancreatic hydrolysate of casein (trypticase peptone, 0.1 mg/ml) and some amino acids (cysteine, tryptophan and methionine, 50 µM each) also inhibited the TPO iodide oxidation reaction completely, whereas casamino acids (0.1 mg/ml), and tyrosine, phenylalanine and histidine (50 µM each) inhibited the TPO reaction by 54% or less. A pancreatic digest of gelatin (0.1 mg/ml) or any other amino acid (50 µM) tested did not significantly decrease TPO activity. The amino acids that impair iodide oxidation also inhibit the TPO albumin iodination activity. The inhibitory amino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid; Thyroperoxidase; Amino acids; Iodide oxidation; Lactoperoxidase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300015

Volume and energy folding landscape of prion protein revealed by pressure
Provedor de dados: 56
Autores: Cordeiro,Y.; Kraineva,J.; Winter,R.; Silva,J.L.
The main hypothesis for prion diseases proposes that the cellular protein (PrP C) can be altered into a misfolded, ß-sheet-rich isoform, the PrP Sc (from scrapie). The formation of this abnormal isoform then triggers the transmissible spongiform encephalopathies. Here, we discuss the use of high pressure as a tool to investigate this structural transition and to populate possible intermediates in the folding/unfolding pathway of the prion protein. The latest findings on the application of high pressure to the cellular prion protein and to the scrapie PrP forms will be summarized in this review, which focuses on the energetic and volumetric properties of prion folding and conversion.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prion; High pressure; Structural conversion; Aggregation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800006

Human umbilical cord blood cells in infarcted rats
Provedor de dados: 56
Autores: Pinho-Ribeiro,V.; Maia,A.C.V.; Werneck-de-Castro,J.P S.; Oliveira,P.F.; Goldenberg,R.C.S.; Campos de Carvalho,A.C.
Therapy with bone marrow-derived cells has been used in ischemic patients with reported success. The aim of this study was to determine the therapeutic efficacy of fresh and frozen human umbilical cord blood cells (hUCB) in Wistar rats submitted to permanent occlusion of the left coronary artery. Three hours after myocardial infarction, 2 x 10(7) hUCB cells or vehicle were administered by intramyocardial injection. The animals were divided into five groups: control (N = 10), sham operated (N = 10), infarcted that received vehicle (N = 9), infarcted treated with cryopreserved hUCB (N = 7), and infarcted treated with fresh hUCB (N = 5). Cardiac function was evaluated by electrocardiogram (ECG) and echocardiogram (ECHO) before cell therapy, and by ECG, ECHO,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell therapy; Fresh and frozen human umbilical cord blood; Acute myocardial infarction; Electrocardiogram; Echocardiogram.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300011

Novel methods for the encapsulation of meglumine antimoniate into liposomes
Provedor de dados: 56
Autores: Frézard,F.; Michalick,M.S.M.; Soares,C.F.; Demicheli,C.
The antimonial drug, meglumine antimoniate, was successfully encapsulated in dehydration-rehydration vesicles and in freeze-dried empty liposomes (FDELs). High encapsulation efficiencies (from 28 to 58%) and low weight ratios of lipids to encapsulated antimony (from 1:0.15 to 1:0.3) were achieved. These formulations, contrary to those obtained by conventional methods, can be stored as intermediate lyophilized forms and reconstituted just before use. The efficacy of FDEL-encapsulated meglumine antimoniate was evaluated in hamsters experimentally infected with Leishmania chagasi. A significant reduction of liver parasite burdens was observed in animals treated with this preparation, when compared to control animals treated with empty liposomes. In contrast,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Liposomes; Glucantime; Meglumine antimoniate; Leishmaniasis; Encapsulation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700016

Utilization of microsatellites for the analysis of genomic alterations in colorectal cancers in Brazil
Provedor de dados: 56
Autores: Fuzikawa,A.K.; Haddad,L.A.; da-Cunha-Melo,J.R.; Brasileiro-Filho,G.; Pena,S.D.J.
Two different pathogenetic mechanisms are proposed for colorectal cancers. One, the so-called "classic pathway", is the most common and depends on multiple additive mutational events (germline and/or somatic) in tumor suppressor genes and oncogenes, frequently involving chromosomal deletions in key genomic regions. Methodologically this pathway is recognizable by the phenomenon of loss of heterozygosity. On the other hand, the "mutator pathway" depends on early mutational loss of the mismatch repair system (germline and/or somatic) leading to accelerated accumulation of gene mutations in critical target genes and progression to malignancy. Methodologically this second pathway is recognizable by the phenomenon of microsatellite instability. The distinction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Colorectal carcinoma; Microsatellite; P53; DCC; Microsatellite instability.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800001

Hydrogen sulfide in posthemorrhagic shock mesenteric lymph drainage alleviates kidney injury in rats
Provedor de dados: 56
Autores: Han,B.; Zhao,Z.G.; Zhang,L.M.; Li,S.G.; Niu,C.Y.
Posthemorrhagic shock mesenteric lymph (PHSML) is a key factor in multiple organ injury following hemorrhagic shock. We investigated the role of hydrogen sulfide (H2S) in PHSML drainage in alleviating acute kidney injury (AKI) by administering D,L-propargylglycine (PPG) and sodium hydrosulfide hydrate (NaHS) to 12 specific pathogen-free male Wistar rats with PHSML drainage. A hemorrhagic shock model was established in 4 experimental groups: shock, shock+drainage, shock+drainage+PPG (45 mg/kg, 0.5 h prehemorrhage), and shock+drainage+NaHS (28 µmol/kg, 0.5 h prehemorrhage). Fluid resuscitation was performed after 1 h of hypotension, and PHMSL was drained in the last three groups for 3 h after resuscitation. Renal function and histomorphology were assessed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemorrhagic shock; Mesenteric lymph; Drainage; Kidney injury; Hydrogen sulfide; Inflammation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700622

Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells
Provedor de dados: 56
Autores: Ishimaru,D.; Marcicano,F.G.P.; Rebello,M.A.
The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 µg/ml PGB2 inhibited virus yield by 60%, at the same dose PGA1 suppressed virus replication by more than 90%. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prostaglandins; Mayaro virus; Vero cells; Protein synthesis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900003

Overexpression of Fc receptor-like 1 associated with B-cell activation during hepatitis B virus infection
Provedor de dados: 56
Autores: Wang,Ke; Pei,Hao; Huang,Biao; Yang,Run-Lin; Wu,Hang-Yuan; Zhu,Xue; Zhu,Lan.
The role of B cells in the pathogenesis of hepatitis B virus (HBV) infection has not been explored in depth. In the present study, the activation status of B cells from peripheral blood of healthy controls (N = 20) and patients with acute hepatitis B (AHB, N = 15) or chronic hepatitis B (CHB, N = 30) was evaluated by measuring the expression levels of B-cell activation markers CD69 and CD86, using quantitative real-time PCR and flow cytometry. Moreover, the potential mechanism underlying B-cell activation during HBV infection was further investigated by analyzing the expression profile of FCRL1, an intrinsic activation molecule of B cells. An elevation in the levels of B-cell activation markers including CD69 and CD86 was observed in the AHB patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B virus; B-cell activation; Fc receptor-like 1; Gene expression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200002

Higher values of triglycerides:HDL-cholesterol ratio hallmark disease severity in children and adolescents with sickle cell anemia
Provedor de dados: 56
Autores: Teixeira,R.S.; Arriaga,M.B.; Terse-Ramos,R.; Ferreira,T.A.; Machado,V.R.; Rissatto-Lago,M.R.; Silveira-Mattos,P.S.; Boa-Sorte,N.; Ladeia,A.M.T.; Andrade,B.B.
Dyslipidemia has been described in sickle cell anemia (SCA) but its association with increased disease severity is unknown. Here, we examined 55 children and adolescents with SCA as well as 41 healthy controls to test the association between the lipid profiles in peripheral blood and markers of hemolysis, inflammation, endothelial function, and SCA-related clinical outcomes. SCA patients exhibited lower levels of total cholesterol (P&lt;0.001), low-density lipoprotein cholesterol (LDL-c) (P&lt;0.001), and high-density lipoprotein cholesterol (HDL-c) (P&lt;0.001), while displaying higher triglyceride (TG) levels and TG/HDL-c ratio values (P&lt;0.001). TG/HDL-c values were positively correlated with lactate dehydrogenase (P=0.047), leukocyte count (P=0.006),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell disease; Lipoproteins; Cholesterol; Triglycerides; Hydroxyurea; Endothelial function.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000612

Bilateral repetitive transcranial magnetic stimulation for treatment-resistant depression: a systematic review and meta-analysis of randomized controlled trials
Provedor de dados: 56
Autores: Zhang,Y.Q.; Zhu,D.; Zhou,X.Y.; Liu,Y.Y.; Qin,B.; Ren,G.P.; Xie,P.
There has been concern regarding the use of controversial paradigms for repetitive transcranial magnetic stimulation (rTMS) to manage treatment-resistant depression (TRD). This meta-analysis assessed the efficacy of bilateral rTMS compared with unilateral and sham rTMS in patients with TRD. PubMed, Embase, CENTRAL, PsycINFO, Web of Science, EAGLE and NTIS databases were searched to identify relevant studies, and randomized controlled trials (RCTs) on bilateral rTMS for TRD patients were included. The response was defined as the primary outcome, and remission was the secondary outcome. Ten RCTs that included 634 patients met the eligibility criteria. The risk ratio (RRs) of both the primary and secondary outcomes of bilateral rTMS showed non-significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Repetitive transcranial magnetic stimulation Treatment-resistant depression Meta-analysis; Randomized controlled trials.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300198

Molecular identification of Sicilian (dß)º-thalassemia associated with ß-thalassemia and hemoglobin S in Brazil
Provedor de dados: 56
Autores: Andrade,T.G. de; Fattori,A.; Saad,S.T.O.; Sonati,M.F.; Costa,F.F.
We describe the clinical and molecular characteristics of two unrelated Brazilian families with an association of the Sicilian form of (dß)º-thalassemia with hemoglobin S and ß-thalassemia. Direct sequencing of the ß-globin gene showed only the hemoglobin S mutation in patient 1 and the ß-thalassemia IVS1-110 in patient 2. The other allele was deleted in both patients and PCR of DNA samples of the breakpoint region of both patients showed a band of approximately 1,150 bp, expected to be observed in the DNA of carriers of Sicilian (dß)º-thalassemia. The nucleotide sequence of this fragment confirmed the Sicilian deletion. There are few reports concerning the Hb S/(dß)º-thalassemia association and patient 2 is the first reported case of Sicilian type of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thalassemia; Sickle cell; Hereditary persistence of fetal hemoglobin; Globin genes; Sicilian thalassemia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800003

Evasion of immune responses by Trypanosoma cruzi, the etiological agent of Chagas disease
Provedor de dados: 56
Autores: DosReis,G.A.
Infection with the protozoan parasite Trypanosoma cruzi leads to Chagas disease, which affects millions of people in Latin America. Infection with T. cruzi cannot be eliminated by the immune system. A better understanding of immune evasion mechanisms is required in order to develop more effective vaccines. During the acute phase, parasites replicate extensively and release immunomodulatory molecules that delay parasite-specific responses mediated by T cells. This immune evasion allows the parasite to spread in the host. In the chronic phase, parasite evasion relies on its replication strategy of hijacking the TGF-β signaling pathway involved in inflammation and tissue regeneration. In this article, the mechanisms of immune evasion described for T. cruzi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; T cells; GPI-anchors; TGF-β; Mucin; Apoptosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200001

Low frequency of p53 mutations in cervical carcinomas among Brazilian women
Provedor de dados: 56
Autores: Pinheiro,N.A.; Villa,L.L.
Human papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene product pRb, respectively. E6 binds to p53 protein promoting its degradation. This is considered to contribute to the oncogenesis of HPV-associated anogenital cancer. On the other hand, in HPV-negative cervical carcinoma, p53 mutations are thought to have a role in the transformation process. A total of 122 HPV-positive cervical carcinoma tissue samples were evaluated for the presence of mutations in exons 5-8 of the p53 gene by single-stranded conformation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single-stranded conformation polymorphism; SSCP; Suppressor gene; HPV; PCR; DNA sequencing.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005

Effect of a leucine-supplemented diet on body composition changes in pregnant rats bearing Walker 256 tumor
Provedor de dados: 56
Autores: Ventrucci,G.; Mello,M.A.R.; Gomes-Marcondes,M.C.C.
Cancer patients present high mobilization of host protein, with a decrease in lean body mass and body fat depletion occurring in parallel to neoplastic growth. Since leucine is one of the principal amino acids used by skeletal muscle for energy, we investigated the changes in body composition of pregnant tumor-bearing rats after a leucine-supplemented diet. Sixty pregnant Wistar rats divided into six groups were fed a normal protein diet (18%, N) or a leucine-supplemented diet (3% L-leucine, L). The pregnant groups were: control (CN), Walker 256 carcinoma-bearing rats (WN), control rats pair-fed with tumor-bearing rats (pfN), leucine-supplemented (CL), leucine-supplemented tumor-bearing (WL), and leucine-supplemented rats pair-fed with tumor-bearing rats...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Walker 256 tumor; Pregnancy; Body composition; Leucine; Carcass nitrogen; Diet supplementation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300006

Risk factors for excess weight loss and hypernatremia in exclusively breast-fed infants
Provedor de dados: 56
Autores: Çaglar,M.K.; Özer,I.; Altugan,F.S.
Data were prospectively obtained from exclusively breast-fed healthy term neonates at birth and from healthy mothers with no obstetric complication to determine risk factors for excess weight loss and hypernatremia in exclusively breast-fed infants. Thirty-four neonates with a weight loss  &gt; or = 10% were diagnosed between April 2001 and January 2005. Six of 18 infants who were eligible for the study had hypernatremia. Breast conditions associated with breast-feeding difficulties (P &lt; 0.05), primiparity (P &lt; 0.005), less than four stools (P &lt; 0.001), pink diaper (P &lt; 0.001), delay at initiation of first breast giving (P &lt; 0.01), birth by cesarean section (P &lt; 0.05), extra heater usage (P &lt; 0.005), extra heater usage among mothers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonate; Dehydration; Breast-feeding; Hypernatremia; Weight loss.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400015

Normative data for the Brazilian population in the Boston Diagnostic Aphasia Examination: influence of schooling
Provedor de dados: 56
Autores: Radanovic,M.; Mansur,L.L.; Scaff,M.
In Neurolinguistics, the use of diagnostic tests developed in other countries can create difficulties in the interpretation of results due to cultural, demographic and linguistic differences. In a country such as Brazil, with great social contrasts, schooling exerts a powerful influence on the abilities of normal individuals. The objective of the present study was to identify the influence of schooling on the performance of normal Brazilian individuals in the Boston Diagnostic Aphasia Examination (BDAE), in order to obtain reference values for the Brazilian population. We studied 107 normal subjects ranging in age from 15 to 84 years (mean ± SD = 47.2 ± 17.6 years), with educational level ranging from 1 to 24 years (9.9 ± 4.8 years). Subjects were compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boston Diagnostic Aphasia Examination; Schooling; Brazilian population.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100019

N-terminal prohormone brain natriuretic peptide (NT-proBNP) as a noninvasive marker for restrictive syndromes
Provedor de dados: 56
Autores: Mady,C.; Fernandes,F.; Ramires,F.J.A.; Nastari,L.; Buck,P.C.; Arteaga,E.; Ianni,B.M.; Salemi,V.M.C.
Constrictive pericarditis (CP) and restrictive cardiomyopathy share many similarities in both their clinical and hemodynamic characteristics and N-terminal prohormone brain natriuretic peptide (NT-proBNP) is a sensitive marker of cardiac diastolic dysfunction. The objectives of the present study were to determine whether serum NT-proBNP was high in patients with endomyocardial fibrosis (EMF) and CP, and to investigate how this relates to diastolic dysfunction. Thirty-three patients were divided into two groups: CP (16 patients) and EMF (17 patients). The control group consisted of 30 healthy individuals. Patients were evaluated by bidimensional echocardiography, with restriction syndrome evaluated by pulsed Doppler of the mitral flow and serum NT-proBNP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Constrictive pericarditis; Endomyocardial fibrosis; NT-proBNP; Restrictive cardiomyopathy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800004

Neo-Timm staining in the thalamus of chronically epileptic rats
Provedor de dados: 56
Autores: Hamani,C.; Paulo,I. de; Mello,L.E.A.M.
The thalamus is an important modulator of seizures and is severely affected in cholinergic models of epilepsy. In the present study, chronically epileptic rats had their brains processed for neo-Timm and acetylcholinesterase two months after the induction of status epilepticus with pilocarpine. Both controls and pilocarpine-treated animals presented neo-Timm staining in the anterodorsal nucleus, laterodorsal nucleus, reticular nucleus, most intralaminar nuclei, nucleus reuniens, and rhomboid nucleus of the thalamus, as well as in the zona incerta. The intensity of neo-Timm staining was similar in control and pilocarpine-treated rats, except for the nucleus reuniens and the rhomboid nucleus, which had a lower intensity of staining in the epileptic group. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Pilocarpine; Zinc; Sprouting; Nucleus reuniens; Midline thalamus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100016

Cerebrospinal fluid-contacting nucleus mediates nociception via release of fractalkine
Provedor de dados: 56
Autores: Zhou,Q.Q.; Chen,S.S.; Zhang,Q.Q.; Liu,P.F.; Fang,H.Z.; Yang,Y.; Zhang,L.C.
Increasing evidence suggests that the cerebrospinal ﬂuid-contacting nucleus (CSF-contacting nucleus) mediates the transduction and regulation of pain signals. However, the precise molecular mechanisms remain unclear. Studies show that release of fractalkine (FKN) from neurons plays a critical role in nerve injury-related pain. We tested the hypothesis that release of FKN from the CSF-contacting nucleus regulates neuropathic pain, in a chronic constriction injury rat model. The results show that FKN is expressed by neurons, via expression of its only receptor CX3CR1 in the microglia. The levels of soluble FKN (sFKN) were markedly upregulated along with the increase in FKN mRNA level in rats subjected to chronic constriction injury. In addition, injection of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebrospinal fluid-contacting nucleus; FKN; Microglia; Neuropathic pain; Chronic constriction injury.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900610

Purification of human albumin by the combination of the method of Cohn with liquid chromatography
Provedor de dados: 56
Autores: Tanaka,K.; Shigueoka,E.M.; Sawatani,E.; Dias,G.A.; Arashiro,F.; Campos,T.C.X.B.; Nakao,H.C.
Large volumes of plasma can be fractionated by the method of Cohn at low cost. However, liquid chromatography is superior in terms of the quality of the product obtained. In order to combine the advantages of each method, we developed an integrated method for the production of human albumin and immunoglobulin G (IgG). The cryoprecipitate was first removed from plasma for the production of factor VIII and the supernatant of the cryoprecipitate was fractionated by the method of Cohn. The first precipitate, containing fractions (F)-I + II + III, was used for the production of IgG by the chromatographic method (see Tanaka K et al. (1998) Brazilian Journal of Medical and Biological Research, 31: 1375-1381). The supernatant of F-I + II + III was submitted to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albumin production; Industrial chromatography; Hemoderivate production; Downstream process; Plasma derivatives.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100003

Presence of ductal carcinoma in situ confers an improved prognosis for patients with T1N0M0 invasive breast carcinoma
Provedor de dados: 56
Autores: Logullo,A.F.; Godoy,A.B.; Mourão-Neto,M.; Simpson,A.J.G.; Nishimoto,I.N.; Brentani,M.M.
We have retrospectively analyzed a series of 155 sequential cases of T1N0M0 ductal carcinomas of which 51 tumors had a ductal carcinoma in situ (DCIS) component for correlation between the presence of DCIS and clinicopathological variables, recurrence and patient survival. No correlations between the presence of DCIS and age, menopausal status, size, estrogen or progesterone receptors were found. High-grade infiltrative tumors tended not to present a DCIS component (P = 0.08). Patients with tumors associated with DCIS form a subgroup with few recurrences (P = 0.003) and good survival (P = 0.008). When tumors were classified by size, an association between large tumors (&gt;1.0 cm) and increased recurrence and shortened overall survival was found. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ductal carcinoma in situ; Invasive breast carcinoma; T1N0M0; Tumor size; Recurrence; Overall survival.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800008

No evidence of association of MUC-1 genetic polymorphism with embryo implantation failure
Provedor de dados: 56
Autores: Dentillo,D.B.; Souza,F.R.P.; Meola,J.; Vieira,G.S.; Yazlle,M.E.H.D.; Goulart,L.R.; Martelli,L.
Pregnancy loss can be caused by several factors involved in human reproduction. Although up to 50% of cases remain unexplained, it has been postulated that the major cause of failed pregnancy is an error of embryo implantation. Transmembrane mucin-1 (MUC-1) is a glycoprotein expressed on the endometrial cell surface which acts as a barrier to implantation. The gene that codes for this molecule is composed of a polymorphic tandem repeat of 60 nucleotides. Our objective was to determine if MUC-1 genetic polymorphism is associated with implantation failure in patients with a history of recurrent abortion. The study was conducted on 10 women aged 25 to 35 years with no history of successful pregnancy and with a diagnosis of infertility. The control group...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mucin-1; Female infertility; Implantation failure; Variable number of tandem repeats; Genetic polymorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600007

Effect of risperidone on proliferation and apoptosis of MC3T3-E1 cells
Provedor de dados: 56
Autores: Zheng,Lei; Yang,Lixia; Zhao,Xin; Long,Niya; Li,Peifan; Wang,Yiming.
This aim of this study was to assess the molecular mechanism of osteoporosis in schizophrenia patients with risperidone use. Here, we investigated the effects of risperidone on cellular proliferation and apoptosis of a preosteoblast cell line, MC3T3-E1. Cell viability and apoptotic rate of MC3T3-E1 were detected by cell counting kit-8 and flow cytometry at a serial dose of risperidone and at different time points, respectively. Bone transformation relevant gene serum osteocalcin (BGP), collagen 1, tumor necrosis factor-α (TNF-α), osteoprotegerin (OPG), and receptor activator of nuclear factor-κB ligand (RANKL) mRNA levels were determined by real-time PCR (qPCR). Their protein expression patterns were evaluated using western blot. The results revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Risperidone; MC3T3-E1 cells; Collagen 1; Tumor necrosis factor-α; Receptor activator of nuclear factor-κB ligand.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300605

Effects of exercise training on autonomic and myocardial dysfunction in streptozotocin-diabetic rats
Provedor de dados: 56
Autores: De Angelis,K.L.D.; Oliveira,A.R.; Dall'Ago,P.; Peixoto,L.R.A.; Gadonski,G.; Lacchini,S.; Fernandes,T.G.; Irigoyen,M.C.
Several investigators have demonstrated that diabetes is associated with autonomic and myocardial dysfunction. Exercise training is an efficient non-pharmacological treatment for cardiac and metabolic diseases. The aim of the present study was to investigate the effects of exercise training on hemodynamic and autonomic diabetic dysfunction. After 1 week of diabetes induction (streptozotocin, 50 mg/kg, iv), male Wistar rats (222 ± 5 g, N = 18) were submitted to exercise training for 10 weeks on a treadmill. Arterial pressure signals were obtained and processed with a data acquisition system. Autonomic function and intrinsic heart rate were studied by injecting methylatropine and propranolol. Left ventricular function was assessed in hearts perfused in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental diabetes; Exercise training; Arterial pressure; Autonomic control; Myocardial contractility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600004

Changes in the electrophysiological parameters of the posterior intestine of Anguilla anguilla (Pisces) induced by oxytocin, urotensin II and aldosterone
Provedor de dados: 56
In view of the importance of the intestine in the osmoregulation of freshwater fishes, we determined the effects of oxytocin, urotensin II (UII), and aldosterone added to the serosal side of the isolated posterior intestine of the freshwater-adapted teleost Anguilla anguilla on electrophysiological parameters. Oxytocin decreased the short-circuit current (SCC) and transepithelial potential difference (TPD) at concentrations of 1 and 10 mU/ml (to 50% and 42% of control values, respectively), but did not alter these parameters at a concentration of 0.1 mU/ml. UII reduced SCC and TPD at concentrations of 10 nM, 50 nM and 100 nM (to 85% of control values), but increased these parameters at the concentration of 500 nM (to 115% of control values). Aldosterone...
Palavras-chave: Intestine; Anguilla anguilla; Oxytocin; Urotensin II; Aldosterone.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100006

Restoration of the rabbit corneal surface after total epithelial debridement and complete limbal excision
Provedor de dados: 56
Autores: Faria-e-Sousa,S.J.; Haddad,A.
How is the corneal epithelium restored when all of it plus the limbus have been eliminated? This investigation explored the possibility that this may be achieved through the conjunctival epithelium. The corneal epithelium of the right eye of 12 rabbits (Oryctolagus cuniculus) was totally scraped followed by surgical excision of the limbus plus 1.0-1.5 mm of the adjacent conjunctiva. Antibiotics and corticosteroids were applied for 1 week after surgery. Histological and immunohistochemical techniques were used to monitor the events taking place on the eye surface 2 weeks and 1, 3 and 6 months thereafter. Initially, the corneal surface was covered by conjunctival-like epithelium. After 1 month and more prominently at 3 and 6 months an epithelium displaying...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Corneal epithelium; Conjunctiva; Stem cells; Epithelium transformation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500005

Isolation and intracellular localization of insulin-like proteins from leaves of Bauhinia variegata
Provedor de dados: 56
Autores: Azevedo,C.R.; Maciel,F.M.; Silva,L.B.; Ferreira,A.T.S.; da Cunha,M.; Machado,O.L.T.; Fernandes,K.V.S.; Oliveira,A.E.A.; Xavier-Filho,J.
Evidence based on immunological cross-reactivity and anti-diabetic properties has suggested the presence of insulin-like peptides in plants. The objective of the present study was to investigate the presence of insulin-like proteins in the leaves of Bauhinia variegata ("pata-de-vaca", "mororó"), a plant widely utilized in popular medicine as an anti-diabetic agent. We show that an insulin-like protein was present in the leaves of this plant. A chloroplast protein with a molecular mass similar to that of bovine insulin was extracted from 2-mm thick 15% SDS-PAGE gels and fractionated with a 2 x 24 cm Sephadex G-50 column. The activity of this insulin-like protein (0.48 mg/mL) on serum glucose levels of four-week-old Swiss albino (CF1) diabetic mice was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bauhinia variegata; Plant insulin; Chloroplast; Medicinal plant.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100007

[RETRACTED ARTICLE] Risk factors for early-onset ventilator-associated pneumonia in aneurysmal subarachnoid hemorrhage patients
Provedor de dados: 56
Autores: Cui,J.B.; Chen,Q.Q.; Liu,T.T.; Li,S.J.
This study aimed to investigate the risk factors related to ventilator-acquired pneumonia (VAP) in aneurysmal subarachnoid hemorrhage (SAH) patients. From January 2011 to December 2015, a single-center retrospective study including 200 SAH patients requiring mechanical ventilation (MV) ≥48 h was performed. The clinical data of these patients were collected and analyzed. The age range of the patients were 41–63 and 72 (36%) were male. The Glasgow coma scale score range was 5–15 and the Simplified Acute Physiology Score II range was 31–52. One hundred and forty-eight (74%) patients had a World Federation of Neurosurgeons (WNFS) score ≥III. Aneurysm was secured with an endovascular coiling procedure in 168 (84%) patients and 94 (47%) patients presented VAP....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ventilator-acquired pneumonia; Aneurysmal subarachnoid hemorrhage; Risk factors; Multivariate analysis; Pathogen.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700605

MicroRNA-143 inhibits cell growth by targeting ERK5 and MAP3K7 in breast cancer
Provedor de dados: 56
Autores: Zhou,L.L.; Dong,J.L.; Huang,G.; Sun,Z.L.; Wu,J.
This study aimed to investigate the function and mechanism of microRNA-143 (miR-143) in the occurrence and development of breast cancer (BC). A total of 30 BC tissues, 30 corresponding noncancerous tissues, and 10 normal control (NC) breast tissues were obtained to detect the levels of miR-143, extracellular signal-regulated kinase 5 (ERK5) and mitogen-activated protein 3 kinase 7 (MAP3K7) using RT-qPCR, western blotting or immunohistochemistry. The correlation of miR-143 with ERK5 or MAP3K7 was evaluated using Pearson correlation analysis. MCF-7 cells were transiently transfected with miR-143 mimic, miR-143 inhibitor, miR-143 mimic/inhibitor + si-ERK5, si-MAP3K7 or si-cyclin D1. Then, cell growth was evaluated by MTT assay and the expressions of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular signal-regulated kinase 5; Mitogen-activated protein 3 kinase 7; MiRNA-143; Breast cancer; Cyclin D1.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800608

Effects of eugenol on resting tension of rat atria
Provedor de dados: 56
Autores: Olivoto,R.R.; Damiani,C.E.N.; Kassouf Silva,I.; Lofrano-Alves,M.S.; Oliveira,M.A.; Fogaça,R.T.H.
In cardiac and skeletal muscle, eugenol (μM range) blocks excitation-contraction coupling. In skeletal muscle, however, larger doses of eugenol (mM range) induce calcium release from the sarcoplasmic reticulum. The effects of eugenol are therefore dependent on its concentration. In this study, we evaluated the effects of eugenol on the contractility of isolated, quiescent atrial trabeculae from male Wistar rats (250-300 g; n=131) and measured atrial ATP content. Eugenol (1, 3, 5, 7, and 10 mM) increased resting tension in a dose-dependent manner. Ryanodine [100 µM; a specific ryanodine receptor (RyR) blocker] and procaine (30 mM; a nonspecific RyR blocker) did not block the increased resting tension induced by eugenol regardless of whether extracellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenol; Atrial trabeculae; Resting tension; Ryanodine; Rat; ATP.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400328

Helicobacter pylori adhesion to gastric epithelial cells is mediated by glycan receptors
Provedor de dados: 56
Autores: Magalhães,A.; Reis,C.A.
Helicobacter pylori adhesion to gastric epithelial cells constitutes a key step in the establishment of a successful infection of the gastric mucosa. The high representation of outer membrane proteins in the bacterial genome suggests the relevance of those proteins in the establishment of profitable interactions with the host gastric cells. Gastric epithelial cells are protected by a mucous layer gel, mainly consisting of the MUC5AC and MUC6 mucins. In addition to this protective role, mucins harbor glycan-rich domains that constitute preferential binding sites of many pathogens. In this article we review the main players in the process of H. pylori adhesion to gastric epithelial cells, which contribute decisively to the high prevalence and chronicity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Bacterial adhesins; Lewis antigens; Glycan receptors; Chronic gastritis; Gastric carcinogenesi.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700002

Antioxidant and free radical scavenging effects in extracts of the medicinal herb Achyrocline satureioides (Lam.) DC. ("marcela")
Provedor de dados: 56
Autores: Desmarchelier,C.; Coussio,J.; Ciccia,G.
Achyrocline satureioides (Lam.) DC. (Compositae) is a medicinal herb used in Argentina, Uruguay, Brazil and Paraguay for its choleretic, antispasmodic and hepatoprotective properties. The presence of the flavonoid quercetin and its derivatives, and of different phenolic acids such as caffeic, chlorogenic and isochlorogenic acids in the aerial parts of this plant has led us to study the antioxidant activity of its extracts using different bioassays. The inhibition of luminol-enhanced chemiluminescence by the aqueous and methanolic extracts was used to show that their total reactive antioxidant potential index (TRAP; in µM Trolox equivalents) was 91.0 ± 15.4 and 128.1 ± 20.1 µM, respectively, while the total antioxidant reactivity index (TAR) was calculated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Achyrocline satureioides; Antioxidant activity; Luminol-enhanced chemiluminescence; Thiobarbituric acid-reactive substances; Hydroperoxide-initiated chemiluminescence; Lipid peroxidation; DNA damage; Reactive oxygen species.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900010

Mycotic aneurysm due to Salmonella species: clinical experiences and review of the literature
Provedor de dados: 56
Autores: Guo,Yiqun; Bai,Yu; Yang,Chunxia; Wang,Peng; Gu,Li.
The mortality of patients with mycotic aneurysms is high, especially in East Asia, and infection by Salmonella species is the most common. Our study aimed to improve prognosis of adult mycotic aneurysms with early diagnosis and accurate treatment. Four adult patients with mycotic aneurysm caused by Salmonella were included and analyzed by single-center retrospective analysis. Cases reported in the literature during the past 10 years were also summarized. The average age of the 4 male patients was 61.25 years, while that of the 53 cases reported in the literature was 65.13 years. Hypertension, diabetes, and atherosclerosis were common complications. Most patients presented fever and experienced pain at the corresponding position of the aneurysm. Laboratory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Mycotic aneurysms; EVAR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900201

Effect of curcumin on the proliferation and apoptosis of hepatic stellate cells
Provedor de dados: 56
Autores: Shu,J.C.; He,Y.J.; Lv,X.; Zhao,J.R.; Zhao,J.; Shen,Y.; Ye,G.R.; Wang,L.X.
This study was designed to investigate the effect of curcumin (diferuloylmethane) on the proliferation and apoptosis of hepatic stellate cells (HSC). The cell line HSC-T6 (1.25 x 10(5) cells/mL) was incubated with curcumin and HSC proliferation was detected by a methyl thiazolyl tetrazolium colorimetric assay. HSC apoptosis was detected by flow cytometry, transmission electron microscope and agarose gel electrophoresis. HSC proliferation was significantly inhibited in a concentration-dependent manner (10.6 to 63.5%) after incubation with 20-100 μM curcumin, compared with a control group. At 20, 40, and 60 μM, after 24 h of incubation, curcumin was associated with a significant increase in the number of HSC in the G2/M phase, and a significant decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin; Hepatic stellate cells; Proliferation; Apoptosis; Hepatic fibrosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200009

Relationship of inflammatory markers and pain in patients with head and neck cancer prior to anticancer therapy
Provedor de dados: 56
Autores: Oliveira,K.G.; von Zeidler,S.V.; Lamas,A.Z.; de Podestá,J.R.V.; Sena,A.; Souza,E.D.; Lenzi,J.; Lemos,E.M.; Gouvea,S.A.; Bissoli,N.S.
Pain is a common symptom in patients with cancer, including those with head and neck cancer (HNC). While studies suggest an association between chronic inflammation and pain, levels of inflammatory cytokines, such as C-reactive protein (CRP) and tumor necrosis factor-alpha (TNF-α), have not been correlated with pain in HNC patients who are not currently undergoing anticancer treatment. The purpose of this study was to examine the relationship between these inflammatory markers and perceived pain in HNC patients prior to anticancer therapy. The study group consisted of 127 HNC patients and 9 healthy controls. Pain was assessed using the Brief Pain Inventory (BPI), and serum levels of CRP and TNF-α were determined using the particle-enhanced turbidimetric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pain; Head and neck cancer; C-reactive protein; Tumor necrosis factor-alpha; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700600

Expression and purification of the immunogenically active fragment B of the Park Williams 8 Corynebacterium diphtheriae strain toxin
Provedor de dados: 56
Autores: Nascimento,D.V.; Lemes,E.M.B.; Queiroz,J.L.S.; Silva Jr.,J.G.; Nascimento,H.J.; Silva,E.D.; Hirata Jr.,R.; Dias,A.A.S.O.; Santos,C.S.; Pereira,G.M.B.; Mattos-Guaraldi,A.L.; Armoa,G.R.G.
The construction of a hexahistidine-tagged version of the B fragment of diphtheria toxin (DTB) represents an important step in the study of the biological properties of DTB because it will permit the production of pure recombinant DTB (rDTB) in less time and with higher yields than currently available. In the present study, the genomic DNA of the Corynebacterium diphtheriae Park Williams 8 (PW8) vaccine strain was used as a template for PCR amplification of the dtb gene. After amplification, the dtb gene was cloned and expressed in competent Escherichia coli M15™ cells using the expression vector pQE-30™. The lysate obtained from transformed E. coli cells containing the rDTB PW8 was clarified by centrifugation and purified by affinity chromatography. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fragment B; Diphtheria toxin; Diphtheria; Dtb gene; E. coli gene expression; Immobilized metal affinity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500007

Apolipoprotein and lipid abnormalities in chronic liver failure
Provedor de dados: 56
Autores: Spósito,A.C.; Vinagre,C.G.; Pandullo,F.L; Mies,S.; Raia,S.; Ramires,J.A.F.
Total serum lipids, as well as apolipoproteins A-I (apo A-I) and B (apo B), were determined in 74 patients with chronic liver failure without cholestasis and in 82 normal subjects. The VLDL, LDL and HDL lipid fractions were reduced in the liver failure group by 36%, 24% and 46%, respectively (P&amp;lt;0.001). Apolipoproteins A-I and B were also reduced by 26% and 25%, respectively (P&amp;lt;0.001). However, the reduction of HDL cholesterol (HDLc) was more pronounced than that of apo A-I and the HDLc:apo A-I ratio was significantly lower in the liver failure group. After separating these patients into groups with plasma albumin lower than 3.0, between 3.0 and 3.5, and higher than 3.5 g/dl, the HDLc:apo A-I ratio was proportional to plasma albumin, but the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic liver disease; Liver failure; Apolipoprotein A; Apolipoprotein B; LCAT; HDL cholesterol; VLDL cholesterol; Lipid metabolism.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100004

Invasive pulmonary fungal infections in patients with connective tissue disease: a retrospective study from northern China
Provedor de dados: 56
Autores: Ge,H.F.; Liu,X.Q.; Zhu,Y.Q.; Chen,H.Q.; Chen,G.Z.
Invasive pulmonary fungal infection (IPFI) is a potentially fatal complication in patients with connective tissue disease (CTD). The current study aimed to uncover the clinical characteristics and risk factors of patients with IPFI-CTD. The files of 2186 CTD patients admitted to a single center in northern China between January 2011 and December 2013 were retrospectively reviewed. A total of 47 CTD patients with IPFI were enrolled into this study and assigned to the CTD-IPFI group, while 47 uninfected CTD patients were assigned to the control group. Clinical manifestations were recorded, and risk factors of IPFI were calculated by stepwise logistical regression analysis. Forty-seven (2.15%) CTD patients developed IPFI. Systemic lupus erythematosus patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Connective tissue diseases; Invasive pulmonary fungal infection; Pulmonary disease; Retrospective study.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000705

Signaled two-way avoidance learning using electrical stimulation of the inferior colliculus as negative reinforcement: effects of visual and auditory cues as warning stimuli
Provedor de dados: 56
Autores: Troncoso,A.C.; Cirilo-Júnior,G.; Sandner,G.; Brandão,M.L.
The inferior colliculus is a primary relay for the processing of auditory information in the brainstem. The inferior colliculus is also part of the so-called brain aversion system as animals learn to switch off the electrical stimulation of this structure. The purpose of the present study was to determine whether associative learning occurs between aversion induced by electrical stimulation of the inferior colliculus and visual and auditory warning stimuli. Rats implanted with electrodes into the central nucleus of the inferior colliculus were placed inside an open-field and thresholds for the escape response to electrical stimulation of the inferior colliculus were determined. The rats were then placed inside a shuttle-box and submitted to a two-way...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inferior colliculus; Active avoidance; Selective attention.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300011

Activity of liver microsomal enzymes during the chronic phase of murine schistosomiasis
Provedor de dados: 56
Autores: Conte,F.P.; Fidalgo-Neto,A.A.; Manhães-Rocha,D.A.; Paumgartten,F.J.R.; De-Oliveira,A.C.A.X.
The effects of schistosomiasis on microsomal enzymes were studied on post-infection day 90 when accumulated damage and fibrosis are most intense but granulomatous reaction around the eggs harbored in the liver is smaller than during the earlier phases. Swiss Webster (SW) and DBA/2 mice of either sex (N = 12 per sex per group) were infected with 100 Schistosoma mansoni cercariae on postnatal day 10 and killed on post-infection day 90. Cytochrome P-450 (CYP) concentration and alkoxyresorufin-O-dealkylases (EROD, MROD, BROD, and PROD), p-nitrophenol-hydroxylase (PNPH), coumarin-7-hydroxylase (COH), and UDP-glucuronosyltransferase (UGT) activities were measured in hepatic microsomes. Age-matched mice of the same sex and strain were used as controls. In S....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Schistosoma mansoni; Pharmacokinetics; Cytochrome P-450; Monooxygenases; Granuloma; Liver microsomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500008

Sucrose ingestion causes opioid analgesia
Provedor de dados: 56
Autores: Segato,F.N.; Castro-Souza,C.; Segato,E.N.; Morato,S.; Coimbra,N.C.
The intake of saccharin solutions for relatively long periods of time causes analgesia in rats, as measured in the hot-plate test, an experimental procedure involving supraspinal components. In order to investigate the effects of sweet substance intake on pain modulation using a different model, male albino Wistar rats weighing 180-200 g received either tap water or sucrose solutions (250 g/l) for 1 day or 14 days as their only source of liquid. Each rat consumed an average of 15.6 g sucrose/day. Their tail withdrawal latencies in the tail-flick test (probably a spinal reflex) were measured immediately before and after this treatment. An analgesia index was calculated from the withdrawal latencies before and after treatment. The indexes (mean ± SEM, N =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antinociception; Sucrose intake; Tail-flick test; Endogenous opioids; Rats; Naloxone.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800011

Changes in clinical and laboratory findings at the time of diagnosis of primary hyperparathyroidism in a University Hospital in São Paulo from 1985 to 2002
Provedor de dados: 56
Autores: Ohe,M.N.; Santos,R.O.; Barros,E.R.; Lage,A.; Kunii,I.S.; Abrahão,M.; Cervantes,O.; Hauache,O.M.; Lazaretti-Castro,M.; Vieira,J.G.H.
In contrast to most developed countries, most patients with primary hyperparathyroidism in Brazil are still symptomatic at diagnosis. However, we have been observing a change in this pattern, especially in the last few years. We evaluated 104 patients, 77 females and 27 males aged 11-79 years (mean: 54.4 years), diagnosed between 1985 and 2002 at a University Hospital. Diagnosis was made on the basis of clinical findings and of high total and/or ionized calcium levels, high or inappropriate levels of intact parathyroid hormone and of surgical findings in 80 patients. Patients were divided into three groups, i.e., patients diagnosed from 1985 to 1989, patients diagnosed from 1990 to 1994, and patients diagnosed from 1995 to 2002. The number of new cases...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Primary hyperparathyroidism; Hypercalcemia; Clinical presentation; Asymptomatic primary hyperparathyroidism; Parathyroid hormone; Hyperparathyroidism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900013

Calcium handling by vascular myocytes in hypertension
Provedor de dados: 56
Calcium ions (Ca2+) trigger the contraction of vascular myocytes and the level of free intracellular Ca2+ within the myocyte is precisely regulated by sequestration and extrusion mechanisms. Extensive evidence indicates that a defect in the regulation of intracellular Ca2+ plays a role in the augmented vascular reactivity characteristic of clinical and experimental hypertension. For example, arteries from spontaneously hypertensive rats (SHR) have an increased contractile sensitivity to extracellular Ca2+ and intracellular Ca2+ levels are elevated in aortic smooth muscle cells of SHR. We hypothesize that these changes are due to an increase in membrane Ca2+ channel density and possibly function in vascular myocytes from hypertensive animals. Several...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Vascular smooth muscle; Hypertension; Calcium; Voltage-operated calcium channels; Vascular reactivity; Bay K 8644.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300004

Quantitative studies of the vasculature of the carotid body in the chronically hypoxic rat
Provedor de dados: 56
Autores: Clarke,J.A.; Daly,M. de B.; Marshall,J.M.; Ead,H.W.; Hennessy,E.M.
The carotid bodies of rats made chronically hypoxic by breathing 12% O2 in a normobaric chamber (inspired PO2 91 mmHg) were compared with those of controls. Serial 5-µm sections of the organs were examined using an interactive image analysis system. The total volume of the carotid bodies was increased by 64%. The total vascular volume rose by 103% and was likely due to an increase in size of the large vessels (&amp;gt;12 µm lumen diameter) because the small vessel (5-12 µm lumen diameter) volume did not increase significantly while the small vessel density tended to decrease. The extravascular volume was increased by 57%. Expressed as a percentage of the total volume of the organ, the total vascular volume did not change, but the small vessel volume was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat carotid body; Morphology; Chronic hypoxia.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300012

Decreased brain-derived neurotrophic factor plasma levels in psoriasis patients
Provedor de dados: 56
Autores: Brunoni,A.R.; Lotufo,P.A.; Sabbag,C.; Goulart,A.C.; Santos,I.S.; Benseñor,I.M.
Brain-derived neurotrophic factor (BDNF) is associated with neuroplasticity and synaptic strength, and is decreased in conditions associated with chronic stress. Nevertheless, BDNF has not yet been investigated in psoriasis, a chronic inflammatory systemic disease that is exacerbated by stress. Therefore, our aim was to determine BDNF plasma levels in psoriasis patients and healthy controls. Adult patients (n=94) presenting with psoriasis for at least 1 year were enrolled, and age- and gender-matched with healthy controls (n=307) from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). Participants had neither a previous history of coronary artery disease nor current episode of major depression. BDNF plasma levels were determined using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain derived neurotrophic factor; Psoriasis; Neurotrophins; Psychological stress; Case-control study.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800711

Effects of emotional reactivity on inhibitory avoidance in the elevated T-maze
Provedor de dados: 56
Autores: Conde,C.; Costa,V.; Tomaz,C.
The possibility of the presence of inter-individual emotional differences and the memory performance of rats was examined in the elevated T-maze. Two kinds of aversively motivated behaviors, inhibitory avoidance and escape learning, were measured. Based on the number of trials to achieve a learning criterion, rats were divided into two subgroups with either low or high avoidance reactivity (LAR or HAR, respectively). Retention test avoidance latencies showed that HAR animals had better avoidance memory (Mann-Whitney rank sum test, P = 0.0035). No such differences were found for the escape component of this test. These data suggest that individual emotional differences affect inhibitory avoidance performance, which may help to explain the dispersion of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Elevated T-maze; High and low avoidance rats; Anxiety; Emotional reactivity.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200012

Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
Provedor de dados: 56
Autores: Azevedo,R.B.; Lacava,Z.G.M.; Miyasaka,C.K.; Chaves,S.B.; Curi,R.
Human and animal immune functions present sex dimorphism that seems to be mainly regulated by sex hormones. In the present study, the activities of the antioxidant enzymes total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were measured in intraperitoneal resident macrophages from adult male and female rats. In addition to comparing males and females, we also examined the regulation of these enzyme activities in macrophages by sex steroids. GSH-Px activity did not differ between male and female macrophages. However, both total SOD and CAT activities were markedly higher in females than in males (83 and 180%). Removal of the gonads in both males and females (comparison between castrated groups) increased the difference in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antioxidant enzymes; Macrophages; Sex hormones; Estrogen; Progesterone; Castration.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500018

Postprandial symptoms in dysmotility-like functional dyspepsia are not related to disturbances of gastric myoelectrical activity
Provedor de dados: 56
Autores: Oba-Kuniyoshi,A.S.; Oliveira Jr.,J.A.; Moraes,E.R.; Troncon,L.E.A.
Gastric dysrhythmias, such as tachy- or bradygastria, have been reported in patients with functional dyspepsia (FD), but their role in symptom production is uncertain. It is also not known whether gastric dysrhythmias in these patients can be elicited by physiological gastric distension with a meal. We investigated the relationships between symptoms after ingestion of different volumes of water following a test meal and gastric dysrhythmias in FD patients. Fourteen patients with dysmotility-like FD and 13 healthy volunteers underwent paired electrogastrography (EGG) studies. Fasted subjects ingested 150 ml of yoghurt with either 150 ml (low volume) or 300 ml (high volume) water in random order. Fasting and fed EGGs with monitoring of symptoms were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional dyspepsia; Gastric myoelectrical activity; Electrogastrography; Postprandial symptoms.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100007

Effects of sciatic nerve transection on ultrastructure, NADPH-diaphorase reaction and serotonin-, tyrosine hydroxylase-, c-Fos-, glucose transporter 1- and 3-like immunoreactivities in frog dorsal root ganglion
Provedor de dados: 56
Autores: Rigon,F.; Rossato,D.; Auler,V.B.; Dal Bosco,L.; Faccioni-Heuser,M.C.; Partata,W.A.
Frogs have been used as an alternative model to study pain mechanisms. Since we did not find any reports on the effects of sciatic nerve transection (SNT) on the ultrastructure and pattern of metabolic substances in frog dorsal root ganglion (DRG) cells, in the present study, 18 adult male frogs (Rana catesbeiana) were divided into three experimental groups: naive (frogs not subjected to surgical manipulation), sham (frogs in which all surgical procedures to expose the sciatic nerve were used except transection of the nerve), and SNT (frogs in which the sciatic nerve was exposed and transected). After 3 days, the bilateral DRG of the sciatic nerve was collected and used for transmission electron microscopy. Immunohistochemistry was used to detect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Axotomy; Immunoreactivity; Histochemistry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600513

The physiological role of AT1 receptors in the ventrolateral medulla
Provedor de dados: 56
Autores: Tagawa,T.; Fontes,M.A.P.; Potts,P.D.; Allen,A.M.; Dampney,R.A.L.
Neurons in the rostral and caudal parts of the ventrolateral medulla (VLM) play a pivotal role in the regulation of sympathetic vasomotor activity and blood pressure. Studies in several species, including humans, have shown that these regions contain a high density of AT1 receptors specifically associated with neurons that regulate the sympathetic vasomotor outflow, or the secretion of vasopressin from the hypothalamus. It is well established that specific activation of AT1 receptors by application of exogenous angiotensin II in the rostral and caudal VLM excites sympathoexcitatory and sympathoinhibitory neurons, respectively, but the physiological role of these receptors in the normal synaptic regulation of VLM neurons is not known. In this paper we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin receptors; Central cardiovascular pathways; Blood pressure; Hypothalamus; Neurotransmitters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600005

Determination of macromolecular exchange and PO2 in the microcirculation: a simple system for in vivo fluorescence and phosphorescence videomicroscopy
Provedor de dados: 56
Autores: Torres,L.N.; Torres Filho,I.P.
We have developed a system with two epi-illumination sources, a DC-regulated lamp for transillumination and mechanical switches for rapid shift of illumination and detection of defined areas (250-750 µm²) by fluorescence and phosphorescence videomicroscopy. The system permits investigation of standard microvascular parameters, vascular permeability as well as intra- and extravascular PO2 by phosphorescence quenching of Pd-meso-tetra (4-carboxyphenyl) porphine (PORPH). A Pechan prism was used to position a defined region over the photomultiplier and TV camera. In order to validate the system for in vivo use, in vitro tests were performed with probes at concentrations that can be found in microvascular studies. Extensive in vitro evaluations were performed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Oxygen; Permeability; Microcirculation; Rat mesentery; Intravital microscopy; Phorphyrin.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100017

Non-invasive messenger RNA transcriptional evaluation in human kidney allograft dysfunction
Provedor de dados: 56
Autores: Joelsons,G.; Domenico,T.; Gonçalves,L.F.; Manfro,R.C.
The aim of the present study was to evaluate messenger RNA expression in kidney allograft recipients. Forty-four kidney transplant recipients were evaluated up to three months after grafting. After transplantation, peripheral blood samples were drawn sequentially for real-time polymerase chain reaction analyses of perforin and TIM-3 genes. Biopsies were obtained to evaluate acute graft dysfunction and interpreted according to the Banff classification. Eight patients presented episodes of acute rejection. Recipients with rejection had significantly higher levels of TIM-3 mRNA transcripts compared to those without rejection (median gene expression 191.2 and 36.9 mRNA relative units, respectively; P&lt;0.0001). Also, perforin gene expression was higher in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney transplantation; Acute rejection; Gene expression; Diagnosis; MRNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700608

Differences in prostate cancer detection between Canadian and Saudi populations
Provedor de dados: 56
Autores: Al-Abdin,O.Z.; Rabah,D.M.; Badr,G.; Kotb,A.; Aprikian,A.
Few studies have addressed racial differences in prostate cancer (PCa) detection between Western and Arabian countries, although PCa has a significantly lower prevalence in Arabic populations compared to Western populations. Therefore, an explanation of this difference is lacking. Serum prostate-specific antigen (PSA) is a valuable marker used to select patients who should undergo prostate biopsies, although the manner in which it is used may require adjustments based on the ethnic population in question. We investigated racial differences in the PCa detection rate between Canadian and Saudi populations. A retrospective analysis was performed of data collected prospectively over 5 consecutive years in urology clinics at the McGill University Health Center...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Serum; PSA; PSA density; Digital rectal examination.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600539

Drugs and lifestyle for the treatment and prevention of coronary artery disease: comparative analysis of the scientific basis
Provedor de dados: 56
Autores: da Luz,P.L.; Nishiyama,M.; Chagas,A.C.P.
In this article, we compare two strategies for atherosclerosis treatment: drugs and healthy lifestyle. Statins are the principal drugs used for the treatment of atherosclerosis. Several secondary prevention studies have demonstrated that statins can significantly reduce cardiovascular events including coronary death, the need for surgical revascularization, stroke, total mortality, as well as fatal and non-fatal myocardial infarction. These results were observed in both men and women, the elderly, smokers and non-smokers, diabetics and hypertensives. Primary prevention studies yielded similar results, although total mortality was not affected. Statins also induce atheroma regression and do not cause cancer. However, many unresolved issues remain, such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Statins; Lifestyle; Coronary disease; Prevention.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000002

DNA-lipid systems: A physical chemistry study
Provedor de dados: 56
Autores: Dias,R.; Antunes,F.; Miguel,M.; Lindman,S.; Lindman,B.
It is well known that the interaction of polyelectrolytes with oppositely charged surfactants leads to an associative phase separation; however, the phase behavior of DNA and oppositely charged surfactants is more strongly associative than observed in other systems. A precipitate is formed with very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. Techniques like phase diagram determinations, fluorescence microscopy, and ellipsometry were used to study these systems. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Cationic surfactants; Catanionic mixtures; Chitosan; Phase behavior; Polyelectrolyte-oppositely charged surfactant systems.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500002

Annual changes in serum calcium and inorganic phosphate levels and correlation with gonadal status of a freshwater murrel, Channa punctatus (Bloch)
Provedor de dados: 56
Autores: Srivastav,S.K.; Srivastav,A.K.
Adult Channa punctatus murrels of both sexes (60-80 g) were collected locally from Ramgarh Lake during the second week of every month (10 individuals of each sex/month) throughout the year. Blood samples were collected and analyzed for serum calcium and phosphate levels by the methods of Trinder (1960) and Fiske and Subbarow (1925), respectively. Gonads were fixed to judge the state of maturation of the fish. Males exhibited no change in serum calcium levels throughout the year in correlation with testicular maturation. However, serum phosphate levels exhibited a rise in correlation with the increased gonadosomatic index. Females showed marked seasonal changes in serum calcium and phosphate levels which were associated with ovarian maturation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Inorganic phosphate; Reproduction; Teleost; Vitellogenesis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800006

Genomic analysis of Brazilian patients with Fabry disease
Provedor de dados: 56
Autores: Pereira,F.S.; Jardim,L.B.; Netto,C.B.; Burin,M.G.; Cecchin,C.; Giugliani,R.; Matte,U.S.
Fabry disease is an X-linked lysosomal disorder due to a-galactosidase A deficiency that causes storage of globotriaosylceramide. The gene coding for this lysosomal enzyme is located on the long arm of the X chromosome, in region Xq21.33-Xq22. Disease progression leads to vascular disease secondary to involvement of kidney, heart and the central nervous system. Detection of female carriers based solely on enzyme assays is often inconclusive. Therefore, mutation analysis is a valuable tool for diagnosis and genetic counseling. Many mutations of the a-galactosidase A gene have been reported with high genetic heterogeneity, being most mutations private found in only one family. The disease is panethnic, and estimates of incidence range from about 1 in 40,000...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fabry disease; Lysosomal disorders; A-Galactosidase A; Globotriaosylceramide storage; GLA gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200002

Arylsulfatase A pseudodeficiency in healthy Brazilian individuals
Provedor de dados: 56
Autores: Pedron,C.G.; Gaspar,P.A.; Giugliani,R.; Pereira,M.L.S.
Molecular alterations associated with arylsulfatase A pseudodeficiency (ASA-PD) were characterized by PCR and restriction endonuclease analysis in a sample of healthy individuals from Brazil. ASA activity was also assayed in all subjects. Two individuals homozygous for the N350S and 1524+95A&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;G mutations were detected, corresponding to a frequency of 1.17% (4 of 324 alleles). The individual frequency of the N350S mutation was 20.7% (71 of 342 alleles) and 7.9% (27 of 342 alleles) for the 1524+95A&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;G mutation. The frequency of the ASA-PD allele in our population was estimated to be 7.9%. This is the first report of ASA-PD allele frequency in a South American population. In addition,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arylsulfatase A; Pseudodeficiency; Metachromatic leukodystrophy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800002

In vitro and in vivo antitumor activity of crude extracts obtained from Brazilian Chromobacterium sp isolates
Provedor de dados: 56
Autores: Menezes,C.B.A.; Silva,B.P.; Sousa,I.M.O.; Ruiz,A.L.T.G.; Spindola,H.M.; Cabral,E.; Eberlin,M.N.; Tinti,S.V.; Carvalho,J.E.; Foglio,M.A.; Fantinatti-Garboggini,F.
Natural products produced by microorganisms have been an important source of new substances and lead compounds for the pharmaceutical industry. Chromobacterium violaceum is a Gram-negative β-proteobacterium, abundant in water and soil in tropical and subtropical regions and it produces violacein, a pigment that has shown great pharmaceutical potential. Crude extracts of five Brazilian isolates of Chromobacterium sp (0.25, 2.5, 25, and 250 µg/mL) were evaluated in an in vitro antitumor activity assay with nine human tumor cells. Secondary metabolic profiles were analyzed by liquid chromatography and electrospray ionization mass spectrometry resulting in the identification of violacein in all extracts, whereas FK228 was detected only in EtCE 308 and EtCE 592...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromobacterium sp; Antitumor activity; Secondary metabolites; Genetic diversity; Violacein.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100065

Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis
Provedor de dados: 56
Autores: Rotta,J.C.G.; Lunardi,C.N.; Tedesco,A.C.
The photogeneration of nitric oxide (NO) using laser flash photolysis was investigated for S-nitroso-glutathione (GSNO) and S-nitroso-N-acetylcysteine (NacySNO) at pH 6.4 (PBS/HCl) and 7.4 (PBS). Irradiation of S-nitrosothiol with light (lambda = 355 nm followed by absorption spectroscopy) resulted in the homolytic decomposition of NacySNO and GSNO to generate radicals (GS· and NacyS·) and NO. The release of NO from donor compounds measured with an ISO-Nometer apparatus was larger at pH 7.4 than pH 6.4. NacySNO was also incorporated into dipalmitoyl-phosphatidylcholine liposomes in the presence and absence of zinc phthalocyanine (ZnPC), a well-known photosensitizer useful for photodynamic therapy. Liposomes are usually used as carriers for hydrophobic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S-nitrosothiols; Nitric oxide; Zinc phthalocyanine; Liposomes; Photodynamic therapy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500005

Relationship between Brazilian airline pilot errors and time of day
Provedor de dados: 56
Autores: de Mello,M.T.; Esteves,A.M.; Pires,M.L.N.; Santos,D.C.; Bittencourt,L.R.A.; Silva,R.S.; Tufik,S.
Flight safety is one of the most important and frequently discussed issues in aviation. Recent accident inquiries have raised questions as to how the work of flight crews is organized and the extent to which these conditions may have been contributing factors to accidents. Fatigue is based on physiologic limitations, which are reflected in performance deficits. The purpose of the present study was to provide an analysis of the periods of the day in which pilots working for a commercial airline presented major errors. Errors made by 515 captains and 472 copilots were analyzed using data from flight operation quality assurance systems. To analyze the times of day (shifts) during which incidents occurred, we divided the light-dark cycle (24:00) in four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Airline pilot errors; Circadian rhythm.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200014

Thyroid hormone regulates protein expression in C6 glioma cells
Provedor de dados: 56
Autores: Trentin,A.G.; Alvarez-Silva,M.
Thyroid hormone (T3) is essential to normal brain development. Previously, we have shown that T3 induces cerebellar astrocyte proliferation. This effect is accompanied by alteration in glial fibrillary acidic protein (GFAP) and fibronectin organization. In the present study, we report that the C6 glioma cell line, which expresses GFAP and is classified as an undifferentiated astrocytic cell type, is a target for T3 action. The C6 monolayers were treated with 50 nM T3 for 3 days, after which the cells were maintained for 2 days without medium changes. In C6 cells, T3 induced the expression of proteins of 107, 73 and 62 kDa. The hormone also up-regulated protein bands of 100 (+50%), 37 (+50%) and 25.5 kDa (+50%) and down-regulated proteins of 94 (-100%),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thyroid hormone; Glioma; Protein synthesis; GFAP; Vimentin; Differentiation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000008

Aerobic exercise training in heart failure: impact on sympathetic hyperactivity and cardiac and skeletal muscle function
Provedor de dados: 56
Autores: Brum,P.C.; Bacurau,A.V.N.; Medeiros,A.; Ferreira,J.C.B.; Vanzelli,A.S.; Negrão,C.E.
Heart failure is a common endpoint for many forms of cardiovascular disease and a significant cause of morbidity and mortality. Chronic neurohumoral excitation (i.e., sympathetic hyperactivity) has been considered to be a hallmark of heart failure and is associated with a poor prognosis, cardiac dysfunction and remodeling, and skeletal myopathy. Aerobic exercise training is efficient in counteracting sympathetic hyperactivity and its toxic effects on cardiac and skeletal muscles. In this review, we describe the effects of aerobic exercise training on sympathetic hyperactivity, skeletal myopathy, as well as cardiac function and remodeling in human and animal heart failure. We also discuss the mechanisms underlying the effects of aerobic exercise training.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Exercise training; Sympathetic hyperactivity; Ventricular function; Cardiac remodeling; Skeletal myopathy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900002

Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees
Provedor de dados: 56
Autores: Torres,A.R.; Sandjo,L.P.; Friedemann,M.T.; Tomazzoli,M.M.; Maraschin,M.; Mello,C.F.; Santos,A.R.S.
In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless bees; Propolis; Antimicrobial activity; S. aureus; M. quadrifasciata quadrifasciata; T. angustula.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600617

Inflammatory and anti-inflammatory effects of soybean agglutinin
Provedor de dados: 56
Soybean agglutinin (SBA) lectin, a protein present in raw soybean meals, can bind to and be extensively endocytosed by intestinal epithelial cells, being nutritionally toxic for most animals. In the present study we show that SBA (5-200 µg/cavity) injected into different cavities of rats induced a typical inflammatory response characterized by dose-dependent exudation and neutrophil migration 4 h after injection. This effect was blocked by pretreatment with glucocorticoid (0.5 mg/kg) or by co-injection of N-acetyl-galactosamine (100 x [M] lectin), but not of other sugars (100 x [M] lectin), suggesting an inflammatory response related to the lectin activity. Neutrophil accumulation was not dependent on a direct effect of SBA on the macrophage population...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lectin; Soybean agglutinin (SBA); Neutrophil migration; Inflammation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700009

Pharmacokinetic and parasitological evaluation of the bone marrow of dogs with visceral leishmaniasis submitted to multiple dose treatment with liposome-encapsulated meglumine antimoniate
Provedor de dados: 56
Autores: Schettini,D.A.; Costa Val,A.P.; Souza,L.F.; Demicheli,C.; Rocha,O.G.F.; Melo,M.N.; Michalick,M.S.M.; Frézard,F.
The aim of the present study was to evaluate the impact of a multiple dose regimen of a liposomal formulation of meglumine antimoniate (LMA) on the pharmacokinetics of antimony in the bone marrow of dogs with visceral leishmaniasis and on the ability of LMA to eliminate parasites from this tissue. Dogs naturally infected with Leishmania chagasi received 4 intravenous doses of either LMA (6.5 mg antimony/kg body weight, N = 9), or empty liposomes (at the same lipid dose as LMA, N = 9) at 4-day intervals. A third group of animals was untreated (N = 8). Before each administration and at different times after treatment, bone marrow was obtained and analyzed for antimony level (LMA group) by electrothermal atomic absorption spectrometry, and for the presence of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Liposome; Meglumine antimoniate; Pharmacokinetics; Leishmaniasis; Dogs; Bone marrow.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200017

The time course of ethanol tolerance: associative learning
Provedor de dados: 56
Autores: Bueno,J.L.O.; Fachini,A.
The effect of different contextual stimuli on different ethanol-induced internal states was investigated during the time course of both the hypothermic effect of the drug and of drug tolerance. Minimitters were surgically implanted in 16 Wistar rats to assess changes in their body temperature under the effect of ethanol. Rat groups were submitted to ethanol or saline trials every other day. The animals were divided into two groups, one receiving a constant dose (CD) of ethanol injected intraperitoneally, and the other receiving increasing doses (ID) during the 10 training sessions. During the ethanol training sessions, conditioned stimuli A (tone) and B (buzzer) were presented at "state +" (35 min after drug injection) and "state -" (170 min after drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol tolerance; Associative learning; Time course; Context stimuli; Internal states.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100012

miR-22 and cerebral microbleeds in brainstem and deep area are associated with depression one month after ischemic stroke
Provedor de dados: 56
Autores: Hu,Jia; Zhou,Wei; Zhou,Zhiming; Yang,Qian; Xu,Junfeng; Dong,Wanli.
In this study, we aimed to explore the relationship among miR-22, deep cerebral microbleeds (CMBs), and post-stroke depression (PSD) 1 month after ischemic stroke. We consecutively recruited 257 patients with first-ever and recurrent acute cerebral infarction and performed PSD diagnosis in accordance with the Diagnostic and Statistical Manual IV criteria for depression. Clinical information, assessments of stroke severity, and imaging data were recorded on admission. We further detected plasma miR-22 using quantitative PCR and analyzed the relationship among miR-22, clinical data, and PSD using SPSS 23.0 software. Logistic regression showed that deep (OR=1.845, 95%CI: 1.006-3.386, P=0.047) and brain stem CMBs (OR=2.652, 95%CI: 1.110–6.921, P=0.040), as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depression; MicroRNA; Stroke; Cerebral microbleeds; Magnetic resonance imaging.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500611

Comparison of Pavlovian serial conditional discrimination in rats and hamsters in the same experimental situation
Provedor de dados: 56
Autores: Bueno,J.L.O.; Figueiredo,T.H.; Mello,F.M.C.
The present study compares behavioral changes between two distinct rodent groups, hamsters (Mesocricetus auratus) and Wistar rats, when submitted in the same homogeneous experimental situations to a serial conditional discrimination procedure which involves water deprivation and the processing of temporal variables. Both hamsters and rats acquired serial positive conditional discrimination as indicated by higher frequencies of magazine-oriented behavior during the tone followed by reinforcement (T+) and preceded by the feature stimulus light (L) and during the empty interval, than during the tone alone not followed by reinforcement (T-). Rats' frequencies of magazine-oriented behavior were high during T+ and T-, initially during training, and decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conditional discrimination; Hamsters; Wistar rats; Behavior repertoire.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200012

Anti-hyperalgesic effect of electroacupuncture in a model of post-incisional pain in rats
Provedor de dados: 56
Autores: Oliveira,R.; Prado,W.A.
Electroacupuncture has been proposed to be a low cost and practical method that allows effective pain management with minimal collateral effects. In this study we have examined the effect of electroacupuncture against the hyperalgesia developed in a model of post-incisional pain in rats. A 1-cm longitudinal incision was made through the skin and fascia of the plantar region of the animal hind paw. Mechanical hyperalgesia in the incision was evaluated 135 min after the surgery with von Frey filaments. The tension threshold was reduced from 75 g (upper limit of the test) to 1.36 ± 0.36 g (mean ± SEM) in control rats. It is shown that a 15-min period of electroacupuncture applied 120 min after surgery to the Zusanli (ST36) and Sanyinjiao (SP6) points, but not...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Electroacupuncture; Post-incisional pain; Hyperalgesia; Naloxone.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800012

Does hepatocellular carcinoma in non-alcoholic steatohepatitis exist in cirrhotic and non-cirrhotic patients?
Provedor de dados: 56
Autores: Chagas,A.L.; Kikuchi,L.O.O.; Oliveira,C.P.M.S.; Vezozzo,D.C.P.; Mello,E.S.; Oliveira,A.C.; Cella,L.C.; Herman,P.; Bachella,T.; Caldwell,S.H.; Alves,V.A.F.; Carrilho,F.J.
Non-alcoholic steatohepatitis (NASH) has been associated with hepatocellular carcinoma (HCC) often arising in histologically advanced disease when steatohepatitis is not active (cryptogenic cirrhosis). Our objective was to characterize patients with HCC and active, histologically defined steatohepatitis. Among 394 patients with HCC detected by ultrasound imaging over 8 years and staged by the Barcelona Clinic Liver Cancer (BCLC) criteria, we identified 7 cases (1.7%) with HCC occurring in the setting of active biopsy-proven NASH. All were negative for other liver diseases such as hepatitis C, hepatitis B, autoimmune hepatitis, Wilson disease, and hemochromatosis. The patients (4 males and 3 females, age 63 ± 13 years) were either overweight (4) or obese...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Hepatocellular carcinoma; Liver cancer; Fatty liver; Steatohepatitis; Cirrhosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000014

Trans fatty acid intake is associated with insulin sensitivity but independently of inflammation
Provedor de dados: 56
Autores: Angelieri,C.T.; Barros,C.R.; Siqueira-Catania,A.; Ferreira,S.R.G.
High saturated and trans fatty acid intake, the typical dietary pattern of Western populations, favors a proinflammatory status that contributes to generating insulin resistance (IR). We examined whether the consumption of these fatty acids was associated with IR and inflammatory markers. In this cross-sectional study, 127 non-diabetic individuals were allocated to a group without IR and 56 to another with IR, defined as homeostasis model assessment-IR (HOMA-IR) &amp;gt;2.71. Diet was assessed using 24-h food recalls. Multiple linear regression was employed to test independent associations with HOMA-IR. The IR group presented worse anthropometric, biochemical and inflammatory profiles. Energy intake was correlated with abdominal circumference and inversely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin resistance; Inflammation; Fatty acids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700009

Relative contribution of expectancy and immediate arousal to the facilitatory effect of an auditory accessory stimulus
Provedor de dados: 56
Autores: Del-Fava,F.; Ribeiro-do-Valle,L.E.
An auditory stimulus speeds up a digital response to a subsequent visual stimulus. This facilitatory effect has been related to the expectancy and the immediate arousal that would be caused by the accessory stimulus. The present study examined the relative contribution of these two influences. In a first and a third experiment a simple reaction time task was used. In a second and fourth experiment a go/no-go reaction time task was used. In each of these experiments, the accessory stimulus preceded the target stimulus by 200 ms for one group of male and female volunteers (G Fix). For another group of similar volunteers (G Var) the accessory stimulus preceded the target stimulus by 200 ms in 25% of the trials, by 1000 ms in 25% of the trials and was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Priming; Arousal; Alertness; Expectancy; Attention.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800006

Development of two potential diagnostic monoclonal antibodies against human cytomegalovirus glycoprotein B
Provedor de dados: 56
Autores: Jun,Fan; Minhuan,Li; Yadan,Ma; Yaping,Huang; Hanying,Liang; Jianhua,Hu; Hangping,Yao; Weihang,Ma.
Human cytomegalovirus glycoprotein B (gB) represents a target for diagnosis and treatment in view of the role it plays in virus entry and spread. Nevertheless, to our knowledge, rare detection of a gB antigen has been reported in transplant patients and limited information is available about diagnostic gB monoclonal antibodies (mAbs). Our aim was to develop gB mAbs with diagnostic potential. Hydrophilic gB peptides (ST: amino acids 27-40, SH: amino acids 81-94) of favorable immunogenicity were synthesized and used to immunize BALB/c mice. Two mAbs, named ZJU-FH6 and ZJU-FE6, were generated by the hybridoma technique and limited serial dilution and then characterized by indirect ELISA, Western blotting, immunoprecipitation, and immunohistochemical staining....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human cytomegalovirus; Glycoprotein B; Monoclonal antibody; Antigen detection; Transplantation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700004

A simple method to assess freezing of gait in Parkinson's disease patients
Provedor de dados: 56
Autores: Popovic,M.B.; Djuric-Jovicic,M.; Radovanovic,S.; Petrovic,I.; Kostic,V.
Freezing of gait (FOG) can be assessed by clinical and instrumental methods. Clinical examination has the advantage of being available to most clinicians; however, it requires experience and may not reveal FOG even for cases confirmed by the medical history. Instrumental methods have an advantage in that they may be used for ambulatory monitoring. The aim of the present study was to describe and evaluate a new instrumental method based on a force sensitive resistor and Pearson's correlation coefficient (Pcc) for the assessment of FOG. Nine patients with Parkinson's disease in the "on" state walked through a corridor, passed through a doorway and made a U-turn. We analyzed 24 FOG episodes by computing the Pcc between one "regular/normal" step and the rest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson's disease; Freezing of gait; Time analysis; Pearson's correlation coefficient.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900011

Identification of sarcopenic obesity in postmenopausal women: a cutoff proposal
Provedor de dados: 56
Autores: Oliveira,R.J.; Bottaro,M.; Júnior,J.T.; Farinatti,P.T.V.; Bezerra,L.A.; Lima,R.M.
Sarcopenic obesity is the combination of reduced fat-free mass (FFM) and increased fat mass (FM) with advancing age but there is lack of clear criteria for its identification. The purposes of the present investigation were: 1) to determine the prevalence of postmenopausal women with reduced FFM relative to their FM and height, and 2) to examine whether there are associations between the proposed classification and health-related variables. A total of 607 women were included in this cross-sectional study and were separated into two subsets: 258 older women with a mean age of 66.8 ± 5.6 years and 349 young women aged 18-40 years (mean age, 29.0 ± 7.5 years). All volunteers underwent body composition assessment by dual-energy X-ray absorptiometry. The FFM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat-free mass; Sarcopenia; Aging; Muscle strength.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100014

Clinical impact of Achromobacter xylosoxidans colonization/infection in patients with cystic fibrosis
Provedor de dados: 56
Autores: Firmida,M.C.; Pereira,R.H.V.; Silva,E.A.S.R.; Marques,E.A.; Lopes,A.J.
The rate of diagnosis of colonization/infection of the airways with Achromobacter xylosoxidans has increased in cystic fibrosis patients, but its clinical significance is still controversial. This retrospective, case-control study aimed to evaluate the clinical impact of A. xylosoxidans colonization/infection in cystic fibrosis patients. Individuals who were chronically colonized/infected (n=10), intermittently colonized/infected (n=15), and never colonized/infected with A. xylosoxidans (n=18) were retrospectively evaluated during two periods that were 2 years apart. Demographic characteristics, clinical data, lung function, and chronic bacterial co-colonization data were evaluated. Of the total study population, 87% were pediatric patients and 65.1% were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic fibrosis; Achromobacter spp.; Achromobacter xylosoxidans; Microbiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400703

High frequency of Y chromosome microdeletions in male infertility patients with 45,X/46,XY mosaicism
Provedor de dados: 56
Autores: Li,Leilei; Zhang,Han; Yang,Yi; Zhang,Hongguo; Wang,Ruixue; Jiang,Yuting; Liu,Ruizhi.
The mosaic 45,X/46,XY karyotype is a common sex chromosomal abnormality in infertile men. Males with this mosaic karyotype can benefit from assisted reproductive therapies, but the transmitted abnormalities contain 45,X aneuploidy as well as Y chromosome microdeletions. The aim of this study was to investigate the clinical and genetic characteristics of infertile men diagnosed with 45,X/46,XY mosaicism in China. Of the 734 infertile men found to carry chromosomal abnormalities, 14 patients were carriers of 45,X/46,XY mosaicism or its variants, giving a prevalence of 0.27% (14/5269) and accounting for 1.91% (14/734) of patients with a chromosomal abnormality. There were ten cases (71.43%, 10/14) of 45,X mosaicism exhibiting AZF microdeletions. Case 1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 45; X/46; XY mosaicism Y chromosome microdeletions Male infertility Azoospermia Oligozoospermia.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300605

Elementary screening of lymph node metastatic-related genes in gastric cancer based on the co-expression network of messenger RNA, microRNA and long non-coding RNA
Provedor de dados: 56
Autores: Song,Zhonghua; Zhao,Wenhua; Cao,Danfeng; Zhang,Jinqing; Chen,Shouhua.
Gastric cancer (GC) is the fifth most common cancer and the third leading cause of cancer-related deaths worldwide. The high mortality might be attributed to delay in detection and is closely related to lymph node metastasis. Therefore, it is of great importance to explore the mechanism of lymph node metastasis and find strategies to block GC metastasis. Messenger RNA (mRNA), microRNA (miRNA) and long non-coding RNA (lncRNA) expression data and clinical data were downloaded from The Cancer Genome Atlas (TCGA) database. A total of 908 differentially expressed factors with variance &gt;0.5 including 542 genes, 42 miRNA, and 324 lncRNA were screened using significant analysis microarray algorithm, and interaction networks were constructed using these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric cancer; Lymph node metastasis; Co-expression network; Modular analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400602

The impact of short daily hemodialysis on anemia and the quality of life in Chinese patients
Provedor de dados: 56
Autores: Jiang,J.L.; Ren,W.; Song,J.; Sun,Q.L.; Xiao,X.Y.; Diao,X.Z.; Huang,Y.H.; Lan,L.; Wang,P.; Hu,Z.
Anemia is a frequent complication in hemodialysis patients. Compared to conventional hemodialysis (CHD), short daily hemodialysis (sDHD) has been reported to be effective in many countries except China. The aim of the present study was to determine whether sDHD could improve anemia and quality of life (QOL) for Chinese outpatients with end-stage renal disease. Twenty-seven patients (16 males/11 females) were converted from CHD to sDHD. All laboratory values were measured before conversion (baseline), at 3 months after conversion (sDHD1), and at 6 months after conversion (sDHD2). The patient's QOL was evaluated at baseline and 6 months after conversion using the Medical Outcomes Study 36-Item Short Form Health Survey (SF-36). Hemoglobin concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Short daily hemodialysis; Anemia; Erythropoietin; Quality of life; End-stage renal disease.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700629

Molecular battles between plant and pathogenic bacteria in the phyllosphere
Provedor de dados: 56
Autores: Baker,C.M.; Chitrakar,R.; Obulareddy,N.; Panchal,S.; Williams,P.; Melotto,M.
The phyllosphere, i.e., the aerial parts of the plant, provides one of the most important niches for microbial colonization. This niche supports the survival and, often, proliferation of microbes such as fungi and bacteria with diverse lifestyles including epiphytes, saprophytes, and pathogens. Although most microbes may complete the life cycle on the leaf surface, pathogens must enter the leaf and multiply aggressively in the leaf interior. Natural surface openings, such as stomata, are important entry sites for bacteria. Stomata are known for their vital role in water transpiration and gas exchange between the plant and the environment that is essential for plant growth. Recent studies have shown that stomata can also play an active role in limiting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guard cell; Plant innate immunity; Plant hormones; Bacterial pathogenesis; Food safety.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800001

Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus
Provedor de dados: 56
Autores: Giraldi-Guimarães,A.; Bittencourt-Navarrete,R.E.; Mendez-Otero,R.
We investigated the level of expression of neuronal nitric oxide synthase (nNOS) in the retinorecipient layers of the rat superior colliculus during early postnatal development. Male and female Lister rats ranging in age between the day of birth (P0) and the fourth postnatal week were used in the present study. Two biochemical methods were used, i.e., in vitro measurement of NOS specific activity by the conversion of [³H]-arginine to [³H]-citrulline, and analysis of Western blotting immunoreactive bands from superior colliculus homogenates. As revealed by Western blotting, very weak immunoreactive bands were observed as early as P0-2, and their intensity increased progressively at least until P21. The analysis of specific activity of NOS showed similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superior colliculus; Nitric oxide synthase; Development; Retinotectal projection.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600013

Low-cost automatic activity data recording system
Provedor de dados: 56
Autores: Moraes,M.F.D.; Ferrarezi,C.; Mont'Alverne,F.J.A.; Garcia-Cairasco,N.
We describe a low-cost, high quality device capable of monitoring indirect activity by detecting touch-release events on a conducting surface, i.e., the animal's cage cover. In addition to the detecting sensor itself, the system includes an IBM PC interface for prompt data storage. The hardware/software design, while serving for other purposes, is used to record the circadian activity rhythm pattern of rats with time in an automated computerized fashion using minimal cost computer equipment (IBM PC XT). Once the sensor detects a touch-release action of the rat in the upper portion of the cage, the interface sends a command to the PC which records the time (hours-minutes-seconds) when the activity occurred. As a result, the computer builds up several files...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circadian rhythm; Automated acquisition; Actograms; FFT; Cosinor; Chronobiology.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800015

Intrinsic denervation of the colon is associated with a decrease of some colonic preneoplastic markers in rats treated with a chemical carcinogen
Provedor de dados: 56
Autores: Vespúcio,M.V.O.; Turatti,A.; Modiano,P.; Oliveira,E.C. de; Chicote,S.R.M.; Pinto,A.M.P.; Garcia,S.B.
Denervation of the colon is protective against the colon cancer; however, the mechanisms involved are unknown. We tested the hypothesis that the denervated colonic mucosa could be less responsive to the action of the chemical carcinogen dimethylhydrazine (DMH). Three groups of 32 male Wistar rats were treated as follows: group 1 (G1) had the colon denervated with 0.3 mL 1.5 mM benzyldimethyltetradecylammonium (benzalkonium chloride, BAC); G2 received a single ip injection of 125 mg/kg DMH; G3 was treated with BAC + the same dose and route of DMH. A control group (Sham, N = 32) did not receive any treatment. Each group was subdivided into four groups according to the sacrifice time (1, 2, 6, and 12 weeks after DMH). Crypt fission index, ß-catenin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon cancer; Dimethylhydrazine; Benzalkonium chloride; Aberrant crypt foci; SS-catenin; Megacolon.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400009

Acute extracellular fluid volume changes increase ileocolonic resistance to saline flow in anesthetized dogs
Provedor de dados: 56
Autores: Santiago Jr.,A.T.; Gondim,F. de-A.A.; Cavalcante,D.I.M.; da-Graça,J.R.V.; de-Oliveira,G.R.; dos-Santos,A.A.; Rola,F.H.
We determined the effect of acute extracellular fluid volume changes on saline flow through 4 gut segments (ileocolonic, ileal, ileocolonic sphincter and proximal colon), perfused at constant pressure in anesthetized dogs. Two different experimental protocols were used: hypervolemia (iv saline infusion, 0.9% NaCl, 20 ml/min, volume up to 5% body weight) and controlled hemorrhage (up to a 50% drop in mean arterial pressure). Mean ileocolonic flow (N = 6) was gradually and significantly decreased during the expansion (17.1%, P&lt;0.05) and expanded (44.9%, P&lt;0.05) periods while mean ileal flow (N = 7) was significantly decreased only during the expanded period (38%, P&lt;0.05). Mean colonic flow (N = 7) was decreased during expansion (12%, P&lt;0.05) but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ileocolonic motility; Extracellular fluid volume; Expansion; Hemorrhage; Atropine.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800014

Contrast sensitivity to angular frequency gratings is not higher than to Cartesian gratings
Provedor de dados: 56
Autores: Zana,Y.; Cavalcanti,A.C.G.T.
When contrast sensitivity functions to Cartesian and angular gratings were compared in previous studies the peak sensitivity to angular stimuli was reported to be 0.21 log units higher. In experiments carried out to repeat this result, we used the same two-alternative forced-choice paradigm, but improved experimental control and precision by increasing contrast resolution from 8 to 12 bits, increasing the screen refresh rate from 30 Hz interlaced to 85 Hz non-interlaced, linearizing the voltage-luminance relation, modulating luminance in frequencies that minimize pixel aliasing, and improving control of the subject's exposure to the stimuli. The contrast sensitivity functions to Cartesian and angular gratings were similar in form and peak sensitivity (2.4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast sensitivity functions; Cartesian and polar coordinates; Radial and angular gratings.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200010

Experimental murine mycobacteriosis: evaluation of the functional activity of alveolar macrophages in thalidomide- treated mice
Provedor de dados: 56
Autores: Arruda,M.S.P.; Richini,V.B.; Oliveira,S.M.A.; Vilani-Moreno,F.R.
Thalidomide is a selective inhibitor of tumor necrosis factor-alpha (TNF-alpha), a cytokine involved in mycobacterial death mechanisms. We investigated the role of this drug in the functional activity of alveolar macrophages in the presence of infection induced by intranasal inoculation of Mycobacterium avium in thalidomide-treated and untreated adult Swiss mice. Sixty animals were inoculated with 5 x 10(6) M. avium by the respiratory route. Thirty animals received daily thalidomide (30 mg/kg mouse) and 30 received water by gavage up to sacrifice. Ten non-inoculated mice were used as a control group. Lots of animals from each group were evaluated until 6 weeks after inoculation. Infection resulted in an increased total number of inflammatory cells as well...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium avium; Alveolar macrophages; Thalidomide; Experimental infection.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400005

Cocaine/levamisole-induced systemic vasculitis with retiform purpura and pauci-immune glomerulonephritis
Provedor de dados: 56
Autores: Veronese,F.V.; Dode,R.S.O.; Friderichs,M.; Thomé,G.G.; Silva,D.R. da; Schaefer,P.G.; Sebben,V.C.; Nicolella,A.R.; Barros,E.J.G.
Levamisole has been increasingly used as an adulterant of cocaine in recent years, emerging as a public health challenge worldwide. Levamisole-associated toxicity manifests clinically as a systemic vasculitis, consisting of cutaneous, hematological, and renal lesions, among others. Purpura retiform, cutaneous necrosis, intravascular thrombosis, neutropenia, and less commonly crescentic nephritis have been described in association with anti-neutrophil cytoplasmic antibodies (ANCAs) and other autoantibodies. Here we report the case of a 49-year-old male who was a chronic cocaine user, and who presented spontaneous weight loss, arthralgia, and 3 weeks before admission purpuric skin lesions in the earlobes and in the anterior thighs. His laboratory tests on...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Cocaine; Levamisole; Systemic vasculitis; ANCA; Retiform purpura; Crescentic glomerulonephritis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500501

The hepatic clearance of recombinant tissue-type plasminogen activator decreases after an inflammatory stimulus
Provedor de dados: 56
Autores: Nagaoka,M.R.; Kouyoumdjian,M.; Borges,D.R.
We have shown that tissue-type plasminogen activator (tPA) and plasma kallikrein share a common pathway for liver clearance and that the hepatic clearance rate of plasma kallikrein increases during the acute-phase (AP) response. We now report the clearance of tPA from the circulation and by the isolated, exsanguinated and in situ perfused rat liver during the AP response (48-h ex-turpentine treatment). For the sake of comparison, the hepatic clearance of a tissue kallikrein and thrombin was also studied. We verified that, in vivo, the clearance of 125I-tPA from the circulation of turpentine-treated rats (2.2 ± 0.2 ml/min, N = 7) decreases significantly (P = 0.016) when compared to normal rats (3.2 ± 0.3 ml/min, N = 6). The AP response does not modify the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver clearance; Inflammation; Kallikrein; Thrombin; Tissue-type plasminogen activator.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100016

Are the current chronic allograft nephropathy grading systems sufficient to predict renal allograft survival?
Provedor de dados: 56
Autores: Moscoso-Solorzano,G.T.; Mastroianni-Kirsztajn,G.; Ozaki,K.S.; Araujo,S.; Franco,M.F.; Pacheco-Silva,A.; Camara,N.O.S.
A major problem in renal transplantation is identifying a grading system that can predict long-term graft survival. The present study determined the extent to which the two existing grading systems (Banff 97 and chronic allograft damage index, CADI) correlate with each other and with graft loss. A total of 161 transplant patient biopsies with chronic allograft nephropathy (CAN) were studied. The samples were coded and evaluated blindly by two pathologists using the two grading systems. Logistic regression analyses were used to evaluate the best predictor index for renal allograft loss. Patients with higher Banff 97 and CADI scores had higher rates of graft loss. Moreover, these measures also correlated with worse renal function and higher proteinuria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin angiotensin-converting enzyme inhibitor; Chronic allograft damage index; Banff 97; Renal allograft survival; Kidney transplantation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000011

T cell receptor excision circles as a tool for evaluating thymic function in young children
Provedor de dados: 56
Autores: Levy,A.; Rangel-Santos,A.; Torres,L.C.; Silveira-Abreu,G.; Agena,F.; Carneiro-Sampaio,M.
The thymus is a primary lymphoid organ responsible for the maturation of T cells as well as the immunological central tolerance. It is in the antenatal period and infancy that it plays its major role. In clinical practice, T cell receptor excision circles (TRECs) are considered a direct and reliable measure of the thymic function. TRECs are a by-product of DNA formation in gene rearrangement of T cell receptors. They are stable and they do not duplicate during mitosis, representing the recent emigrant T cells from the thymus. Despite their importance, TRECs have been neglected by physicians and there is a lack of data regarding thymic function during infancy of healthy children. In order to evaluate thymic function in the first years of life, we propose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human thymus; TRECs; Thymic function; Infants.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700601

Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members
Provedor de dados: 56
Autores: Heinen,R.C.; Diniz-Mendes,L.; Silva,J.T.; Paschoalin,V.M.F.
Yeast soluble proteins were fractionated by calmodulin-agarose affinity chromatography and the Ca2+/calmodulin-binding proteins were analyzed by SDS-PAGE. One prominent protein of 66 kDa was excised from the gel, digested with trypsin and the masses of the resultant fragments were determined by MALDI/MS. Twenty-one of 38 monoisotopic peptide masses obtained after tryptic digestion were matched to the heat shock protein Ssb1/Hsp75, covering 37% of its sequence. Computational analysis of the primary structure of Ssb1/Hsp75 identified a unique potential amphipathic alpha-helix in its N-terminal ATPase domain with features of target regions for Ca2+/calmodulin binding. This region, which shares 89% similarity to the experimentally determined calmodulin-binding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ssb1/Hsp75; Ca2+/calmodulin complex; Hsc70; Calmodulin-affinity peptide; Bag1; Saccharomyces cerevisiae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100003

Roles of estrogen and progesterone in modulating renal nerve function in the rat kidney
Provedor de dados: 56
Autores: Graceli,J.B.; Cicilini,M.A.; Bissoli,N.S.; Abreu,G.R.; Moysés,M.R.
The maintenance of extracellular Na+ and Cl- concentrations in mammals depends, at least in part, on renal function. It has been shown that neural and endocrine mechanisms regulate extracellular fluid volume and transport of electrolytes along nephrons. Studies of sex hormones and renal nerves suggested that sex hormones modulate renal function, although this relationship is not well understood in the kidney. To better understand the role of these hormones on the effects that renal nerves have on Na+ and Cl- reabsorption, we studied the effects of renal denervation and oophorectomy in female rats. Oophorectomized (OVX) rats received 17β-estradiol benzoate (OVE, 2.0 mg·kg-1·day-1, sc) and progesterone (OVP, 1.7 mg·kg-1·day-1,sc). We assessed Na+ and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogen; Na+ and Cl- reabsorption; Progesterone; Renal nerve.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600521

Effect of fatty acids on leukocyte function
Provedor de dados: 56
Autores: Pompéia,C.; Lopes,L.R.; Miyasaka,C.K.; Procópio,J.; Sannomiya,P.; Curi,R.
Fatty acids have various effects on immune and inflammatory responses, acting as intracellular and intercellular mediators. Polyunsaturated fatty acids (PUFAs) of the omega-3 family have overall suppressive effects, inhibiting lymphocyte proliferation, antibody and cytokine production, adhesion molecule expression, natural killer cell activity and triggering cell death. The omega-6 PUFAs have both inhibitory and stimulatory effects. The most studied of these is arachidonic acid that can be oxidized to eicosanoids, such as prostaglandins, leukotrienes and thromboxanes, all of which are potent mediators of inflammation. Nevertheless, it has been found that many of the effects of PUFA on immune and inflammatory responses are not dependent on eicosanoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids; Immune system; Inflammation; Dietary lipids; Leukocytes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100001

Antioxidant status of dog aqueous humor after extracapsular lens extraction
Provedor de dados: 56
Autores: Barros,P.S.M.; Padovani,C.F.; Silva,V.V.; Queiroz,L.; Barros,S.B.M.
We determined the antioxidant status of the aqueous humor after extracapsular lens extraction in 14 mongrel dogs weighing about 10 kg. The animals were examined by slit lamp biomicroscopy, applanation tonometry and indirect ophthalmoscopy. One eye was submitted to conventional extracapsular lens extraction and the other was used as control. Samples of aqueous humor were obtained by anterior chamber paracentesis before and at days 1, 2, 3, 7 and 15 after surgery. Total antioxidant status was determined as the capacity of aqueous humor to inhibit free radical generation by 2,2-azobis(2-amidopropane) chlorine. Ascorbic acid concentration was measured by HPLC with UV detection. Protein content was determined with the biuret reagent. Statistical analysis was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lens; Aqueous humor; Oxidative stress; Antioxidant; Extracapsular lens extraction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100007

Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
Provedor de dados: 56
Autores: Shulzhenko,N.; Morgun,A.; Franco,M.; Souza,M.M.; Almeida,D.R.; Diniz,R.V.Z.; Carvalho,A.C.C.; Pacheco-Silva,A.; Gerbase-DeLima,M.
The purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rejection; Cardiac allograft; Immunologic monitoring; MRNA; Gene activation; Interferon-gamma.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600013

Malaria vaccine: roadblocks and possible solutions
Provedor de dados: 56
Autores: Soares,I.S.; Rodrigues,M.M.
Malaria remains the most prevalent and devastating parasitic disease worldwide. Vaccination is considered to be an approach that will complement other strategies for prevention and control of the disease in the future. In the last 10 years, intense studies aimed at the development of a malaria vaccine have provided important knowledge of the nature of the host immunological mechanisms of protection and their respective target antigens. It became well established that protective immune responses can be generated against the distinct stages of Plasmodium. However, in general, protective immune responses are directed at stage-specific antigens. The elucidation of the primary structure of these antigens made possible the generation of synthetic and recombinant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Plasmodium; Vaccine; Immunity; Infectious disease.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300002

Oxidative stress in the latissimus dorsi muscle of diabetic rats
Provedor de dados: 56
Autores: De Angelis,K.L.D.; Cestari,I.A.; Barp,J.; Dall'Ago,P.; Fernandes,T.G.; Homem de Bittencourt,P.I.; Belló-Klein,A.; Belló,A.A.; Llesuy,S.; Irigoyen,M.C.
The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 ± 5 g, N = 11). Short-term (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia &gt;300 mg/dl). LD muscle of STZ-diabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 ± 0.02 nmol/mg protein and 14706 ± 1581 cps/mg protein) than LD muscle of normal rats (0.23 ± 0.04 nmol/mg protein and 7389 ± 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Streptozotocin-diabetes; Latissimus dorsi muscle; Oxidative stress; Catalase; Glutathione.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100016

Maturation of pig oocytes in vitro in a medium with pyruvate
Provedor de dados: 56
Autores: Gonzales-Figueroa,H.; Gonzales-Molfino,H.M.
The aim of in vitro maturation oocyte systems is to produce oocytes of comparable quality to those derived in vivo. The present study was designed to examine the surface morphological changes of the cumulus-oocyte complex (COC) and nuclear maturation in a culture system containing pyruvate. Ovaries were obtained from a slaughterhouseand transported to the laboratory within 2 h at 35-39ºC,and rinsed three times in 0.9% NaCl. The COCs were harvested from the ovaries and in vitro maturation was evaluated in San Marcos (SM) medium, a chemically defined culture system containing 22.3 mM sodium pyruvate. Oocytes were cultured in SM, SM + porcine follicular fluid (pFF) and in SM + pFF + gonadotropins (eCG and hCG) for 20-22 h and then without hormonal supplements...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pig oocytes; In vitro maturation; Pyruvate; Cumulus cells.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600008

AKT inhibitor suppresses hyperthermia-induced Ndrg2 phosphorylation in gastric cancer cells
Provedor de dados: 56
Autores: Tao,Yurong; Guo,Yan; Liu,Wenchao; Zhang,Jian; Li,Xia; Shen,Lan; Ru,Yi; Xue,Yan; Zheng,Jin; Liu,Xinping; Zhang,Jing; Yao,Libo.
Hyperthermia is one of the most effective adjuvant treatments for various cancers with few side effects. However, the underlying molecular mechanisms still are not known. N-myc downstream-regulated gene 2 (NDRG2), a tumor suppressor, has been shown to be involved in diverse cellular stresses including hypoxia, lipotoxicity, etc. In addition, Ndrg2 has been reported to be related to progression of gastric cancer. In the current study, our data showed that the apoptosis rate of MKN28 cells increased relatively rapidly to 13.4% by 24 h after treatment with hyperthermia (42°C for 1 h) compared to 5.1% in control cells (P &lt; 0.05). Nevertheless, there was no obvious change in the expression level of total Ndrg2 during this process. Further investigation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperthermia; Gastric cancer; Ndrg2; Apoptosis; AKT inhibitor.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400394

Ambient particulate air pollution from vehicles promotes lipid peroxidation and inflammatory responses in rat lung
Provedor de dados: 56
Autores: Pereira,C.E.L.; Heck,T.G.; Saldiva,P.H.N.; Rhoden,C.R.
Oxidative stress plays a major role in the pathogenesis of particle-dependent lung injury. Ambient particle levels from vehicles have not been previously shown to cause oxidative stress to the lungs. The present study was conducted to a) determine whether short-term exposure to ambient levels of particulate air pollution from vehicles elicits inflammatory responses and lipid peroxidation in rat lungs, and b) determine if intermittent short-term exposures (every 4 days) induce some degree of tolerance. Three-month-old male Wistar rats were exposed to ambient particulate matter (PM) from vehicles (N = 30) for 6 or 20 continuous hours, or for intermittent (5 h) periods during 20 h for 4 consecutive days or to filtered air (PM &amp;lt;10 µm; N = 30). Rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Reactive oxygen species; Lipid peroxidation; Particulate air pollution; Lung inflammation; Acute effects.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000008

Dynamics of immunosuppression in hamsters with experimental visceral leishmaniasis
Provedor de dados: 56
Autores: Fazzani,C.; Guedes,P.A.; Senna,A.; Souza,E.B.; Goto,H.; Lindoso,J.A.L.
Immunosuppression has been reported to occur during active visceral leishmaniasis and some factors such as the cytokine profile may be involved in this process. In the mouse model of cutaneous leishmaniasis using Leishmania (Leishmania) major, the Th1 response is related to protection while the Th2 response is related to disease progression. However, in hamsters, which are considered to be an excellent model for the study of visceral leishmaniasis, this dichotomy is not observed. Using outbred 45- to 60-day-old (140 to 150 g) male hamsters infected intraperitoneally with 2 x 10(7) L. (L.) chagasi amastigotes, we evaluated the immune response of spleen cells and the production of cytokines. We used 3 to 7 hamsters per group evaluated. We detected a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunosuppression; Leishmania(Leishmania) chagasi; Hamster; Cytokine; T-cell proliferation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700009

Association between NOx exposure and deaths caused by respiratory diseases in a medium-sized Brazilian city
Provedor de dados: 56
Autores: César,A. C. G.; Carvalho Jr.,J. A.; Nascimento,L. F. C.
Exposure to nitrogen oxides (NOx) emitted by burning fossil fuels has been associated with respiratory diseases. We aimed to estimate the effects of NOx exposure on mortality owing to respiratory diseases in residents of Taubaté, São Paulo, Brazil, of all ages and both sexes. This time-series ecological study from August 1, 2011 to July 31, 2012 used information on deaths caused by respiratory diseases obtained from the Health Department of Taubaté. Estimated daily levels of pollutants (NOx, particulate matter, ozone, carbon monoxide) were obtained from the Centro de Previsão de Tempo e Estudos Climáticos Coupled Aerosol and Tracer Transport model to the Brazilian developments on the Regional Atmospheric Modeling System. These environmental variables were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollution; Nitrogen oxides; Respiratory diseases; Death.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201130

The use of genes for performance enhancement: doping or therapy?
Provedor de dados: 56
Autores: Oliveira,R.S.; Collares,T.F.; Smith,K.R.; Collares,T.V.; Seixas,F.K.
Recent biotechnological advances have permitted the manipulation of genetic sequences to treat several diseases in a process called gene therapy. However, the advance of gene therapy has opened the door to the possibility of using genetic manipulation (GM) to enhance athletic performance. In such ‘gene doping’, exogenous genetic sequences are inserted into a specific tissue, altering cellular gene activity or leading to the expression of a protein product. The exogenous genes most likely to be utilized for gene doping include erythropoietin (EPO), vascular endothelial growth factor (VEGF), insulin-like growth factor type 1 (IGF-1), myostatin antagonists, and endorphin. However, many other genes could also be used, such as those involved in glucose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic manipulation; Erythropoietin; Vascular endothelial growth factor; Insulin-like growth factor-1; Myostatin; Bioethics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200001

A routine electrocardiogram cannot be used to determine the size of myocardial infarction in the rat
Provedor de dados: 56
Autores: Bonilha,A.M.M.; Saraiva,R.M.; Kanashiro,R.M.; Portes,L.A.; Antonio,E.L.; Tucci,P.J.F.
Nine lead electrocardiograms of non-infarcted (N = 61) and infarcted (N = 71) female Wistar rats (200-250 g) were analyzed in order to distinguish left ventricle myocardial infarction (MI) larger than 40% (LMI) from MI smaller than 40% (SMI). MI larger than 40% clearly caused a deviation of ÂQRS and ÂT from normal values of 270-360 degrees to 90-270 degrees. Infarcted rats showed Q wave in D1 larger than 1 mm with 94% sensitivity and 100% specificity. The sum of QRS positivity in V1, V2 and V6 lower than 10 mm identified MI with 82% sensitivity and 100% specificity. The data showed that MI can be easily and reliably diagnosed by electrocardiogram in the rat. However, contradicting what is frequently believed, when specificity and sensitivity were analyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction size; Rats; Electrocardiogram; Echocardiogram; ROC curve.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400016

Acute effects of selective serotonin reuptake inhibitors on neuroleptic-induced catalepsy in mice
Provedor de dados: 56
Autores: Pires,J.G.P.; Bonikovski,V.; Futuro-Neto,H.A.
Depression found in Parkinson disease (PD) usually responds to selective serotonin reuptake inhibitors (SSRIs). Drugs that modify experimental neuroleptic catalepsy (NC) might affect extrapyramidal symptoms in PD. Therefore, the effects of SSRIs on NC were tested in mice, 26-36 g, separated by sex. Catalepsy was induced with haloperidol (H; 1 mg/kg, ip) and measured at 30-min intervals using a bar test. An SSRI (sertraline, ST; paroxetine, PX; fluoxetine) or vehicle (C) was injected ip 30 min before H. Dunnett's test was used for comparison of means. ST (1-5 mg/kg) or PX (1-5 mg/kg) attenuated NC, with a similar inhibition found in both sexes (5 mg/kg, 180 min: ST - males: 124 ± 10 vs 714 ± 15 s in C; females: 116 ± 10 vs 718 ± 6 s in C; PX - males: 106 ±...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Selective serotonin reuptake inhibitors; Fluoxetine; Sertraline; Paroxetine; Extrapyramidal system; Catalepsy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200015

Aggrecan structure in amphibian cartilage
Provedor de dados: 56
Autores: Covizi,D.Z.; Carvalho,H.F.
The structure of the large proteoglycan present in the bullfrog epiphyseal cartilage was studied by immunochemical and biochemical methods. The isolated monomer showed a polydisperse behavior on Sepharose CL2B, with a peak at Kav = 0.14. Chondroitin sulfate chains were identified by HPLC analysis of the products formed by chondroitinase digestion and mercuric acetate treatment. These chains have approximately 38 disaccharides, a Di45:Di68 ratio of 1.6 and GalNAc4S + GalNAc4,6S are the main non-reducing terminals. Keratan sulfate was identified by the use of two monoclonal antibodies in Western blots after chondroitinase ABC treatment. A keratan sulfate-rich region (~110 kDa) was isolated by sequential treatment with chondroitinase ABC and proteases. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aggrecan; Bullfrog; Cartilage; Chondroitin sulfate; Epiphyseal cartilage.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200002

Cerebral vasomotor reactivity assessment using Transcranial Doppler and MRI with apnea test
Provedor de dados: 56
Autores: Herrera Campos,C.R.; Beltramini,G.C.; Avelar,W.M.; Lima,F.O.; Li,L.M.
Differently from previous studies that used Transcranial Doppler (TCD) and functional MRI (fMRI) for cerebral vasomotor reactivity (CVR) assessment in patients with carotid stenosis (CS), we assessed CVR using an identical stimulus, the Breath-Holding Test (BHT). We included 15 patients with CS and 7 age-matched controls to verify whether fMRI responded differently to BHT between groups and to calculate the agreement rate between tests. For TCD, impaired CVR was defined when the mean percentage increase on middle cerebral artery velocities was ≤31% on 3 consecutive 30-s apnea intercalated by 4-min normal breathing intervals. For fMRI, the percent variation on blood oxygen level-dependent (BOLD) signal intensity in the lentiform nucleus (LN) ipsilateral to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood oxygen level dependence; Transcranial Doppler; Carotid stenosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100704

Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+
Provedor de dados: 56
Autores: Faria,D.P.; Buchpiguel,C.A.; Marques,F.L.N.
Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI)6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75), Whatman 3MM paper and ethanol:chloroform...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals; Technetium-99m; Quality control; Chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000007

35 years of the Brazilian Journal of Medical and Biological Research
Provedor de dados: 56
Autores: Rego,E.M.; Pereira Leite,J.; Bensenor,I.; Chammas,R.; Nogueira de Francischi,J.; da Luz,P.L.
The authors pay homage to the three founders of the Brazilian Journal of Medical and Biological Research Profs. Lewis Joel Greene, Sérgio Henrique Ferreira and Eduardo Moacyr Krieger for their vision and commitment to divulge the scientific production of developing countries.
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100101

Prevalence of child malnutrition at a university hospital using the World Health Organization criteria and bioelectrical impedance data
Provedor de dados: 56
Autores: Pileggi,V.N.; Monteiro,J.P.; Margutti,A.V.B.; Camelo Jr.,J.S.
Malnutrition constitutes a major public health concern worldwide and serves as an indicator of hospitalized patients’ prognosis. Although various methods with which to conduct nutritional assessments exist, large hospitals seldom employ them to diagnose malnutrition. The aim of this study was to understand the prevalence of child malnutrition at the University Hospital of the Ribeirão Preto Medical School, University of São, Brazil. A cross-sectional descriptive study was conducted to compare the nutritional status of 292 hospitalized children with that of a healthy control group (n=234). Information regarding patients’ weight, height, and bioelectrical impedance (i.e., bioelectrical impedance vector analysis) was obtained, and the phase angle was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Child malnutrition; Prevalence; Bioelectrical impedance.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300705

Glutamine and glutamate as vital metabolites
Provedor de dados: 56
Autores: Newsholme,P.; Lima,M.M.R.; Procopio,J.; Pithon-Curi,T.C.; Doi,S.Q.; Bazotte,R.B.; Curi,R.
Glucose is widely accepted as the primary nutrient for the maintenance and promotion of cell function. This metabolite leads to production of ATP, NADPH and precursors for the synthesis of macromolecules such as nucleic acids and phospholipids. We propose that, in addition to glucose, the 5-carbon amino acids glutamine and glutamate should be considered to be equally important for maintenance and promotion of cell function. The functions of glutamine/glutamate are many, i.e., they are substrates for protein synthesis, anabolic precursors for muscle growth, they regulate acid-base balance in the kidney, they are substrates for ureagenesis in the liver and for hepatic and renal gluconeogenesis, they act as an oxidative fuel for the intestine and cells of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamine; Glutamate; Glucose; Metabolism; Cell function.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200002

Inspiratory flow-volume curve in snoring patients with and without obstructive sleep apnea
Provedor de dados: 56
Autores: Amado,V.M.; Costa,A.C.G.A.; Guiot,M.; Viegas,C.A.; Tavares,P.
We analyzed the flow-volume curves of 50 patients with complaints of snoring and daytime sleepiness in treatment at the Pneumology Unit of the University Hospital of Brasília. The total group was divided into snorers without obstructive sleep apnea (OSA) (N = 19) and snorers with OSA (N = 31); the patients with OSA were subdivided into two groups according to the apnea/hypopnea index (AHI): AHI&amp;lt;20/h (N = 14) and AHI&amp;gt;20/h (N = 17). The control group (N = 10) consisted of nonsmoking subjects without complaints of snoring, daytime sleepiness or pulmonary diseases. The population studied (control and patients) consisted of males of similar age, height and body mass index (BMI); spirometric data were also similar in the four groups. There was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep; Flow-volume curve; Obstructive sleep apnea; Snoring; Upper airway resistance.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400005

Striatal and extrastriatal atrophy in Huntington's disease and its relationship with length of the CAG repeat
Provedor de dados: 56
Autores: Ruocco,H.H.; Lopes-Cendes,I.; Li,L.M.; Santos-Silva,M.; Cendes,F.
Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder that affects the striatum most severely. However, except for juvenile forms, relative preservation of the cerebellum has been reported. The objective of the present study was to perform MRI measurements of caudate, putamen, cerebral, and cerebellar volumes and correlate these findings with the length of the CAG repeat and clinical parameters. We evaluated 50 consecutive patients with HD using MRI volumetric measurements and compared them to normal controls. Age at onset of the disease ranged from 4 to 73 years (mean: 43.1 years). The length of the CAG repeat ranged from 40 to 69 (mean: 47.2 CAG). HD patients presented marked atrophy of the caudate and putamen, as well as reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurodegeneration; Dynamic mutation; Genotype-phenotype correlation; Basal ganglia; Magnetic resonance imaging; Huntington's disease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800016

The methyl ester of rosuvastatin elicited an endothelium-independent and 3-hydroxy-3-methylglutaryl coenzyme A reductase-independent relaxant effect in rat aorta
Provedor de dados: 56
Autores: López-Canales,J.S.; López-Sanchez,P.; Perez-Alvarez,V.M.; Wens-Flores,I.; Polanco,A.C.; Castillo-Henkel,E.; Castillo-Henkel,C.
The relaxant effect of the methyl ester of rosuvastatin was evaluated on aortic rings from male Wistar rats (250-300 g, 6 rats for each experimental group) with and without endothelium precontracted with 1.0 µM phenylephrine. The methyl ester presented a slightly greater potency than rosuvastatin in relaxing aortic rings, with log IC50 values of -6.88 and -6.07 M, respectively. Unlike rosuvastatin, the effect of its methyl ester was endothelium-independent. Pretreatment with 10 µM indomethacin did not inhibit, and pretreatment with 1 mM mevalonate only modestly inhibited the relaxant effect of the methyl ester. Nω-nitro-L-arginine methyl ester (L-NAME, 10 µM), the selective nitric oxide-2 (NO-2) inhibitor 1400 W (10 µM), tetraethylammonium (TEA, 10 mM),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat aorta; Rosuvastatin methyl ester; Endothelium-independent relaxation; Potassium channels; NOS-2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500009

A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK) gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits
Provedor de dados: 56
Autores: Silva,S.C.; Brum,M.C.S.; Weiblen,R.; Flores,E.F.; Chowdhury,S.I.
Bovine herpesvirus 5 (BoHV-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 strain (SV507/99). The recombinants are defective in glycoprotein E (BoHV-5gEΔ), thymidine kinase (BoHV-5TKΔ) and both proteins (BoHV-5gEΔTKΔ). Rabbits inoculated with the parental virus (N = 8) developed neurological disease and died or were euthanized in extremis between days 7 and 13 post-infection (pi). Infectivity was detected in several areas of their brains. Three of 8 rabbits inoculated with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-5; GE and TK deletion mutants; Pathogenesis; Virulence; Vaccine candidate.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200005

Diagnostic profile of inpatients as a determinant of length of stay in a general hospital psychiatric unit
Provedor de dados: 56
Autores: Hallak,J.E.C.; Crippa,J.A.S.; Vansan,G.; Zuardi,A.W.
The aim of this study was to determine if the diagnostic profile of inpatients of a psychiatric unit in a general hospital influences the length of stay. The results of a retrospective survey comprising the first 16 years of operation of the Psychiatric Unit of the Ribeirão Preto General Hospital (PURP) showed that the progressive increase observed in the length of stay correlated with the increase in percentage of schizophrenia diagnosis, after the 8th year of hospital operation, and of affective disorders, after the 12th year. The length of hospitalization kept increasing until the 16th year, even though there was no change in the diagnostic profile of the patients admitted to the unit. In a prospective study encompassing the next six months, 61...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychiatric unit; General hospital; Diagnostic category; Length of stay.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900014

Polymorphism of the promoter region and exon 1 of the CTLA4 gene in endemic pemphigus foliaceus (fogo selvagem)
Provedor de dados: 56
Autores: Pavoni,D.P.; Cerqueira,L.B.; Roxo,V.M.M.S.; Petzl-Erler,M.L.
Endemic pemphigus foliaceus (EPF) is an autoimmune bullous skin disease characterized by acantholysis and antibodies against a desmosomal protein, desmoglein 1. Genetic and environmental factors contribute to development of this multifactorial disease. HLA class II and some cytokine gene polymorphisms are the only genetic markers thus far known to be associated with susceptibility to or protection from EPF. The cytotoxic T-lymphocyte antigen-4 gene (CTLA4) encodes a key immunoreceptor molecule that regulates and inhibits T-cell proliferation. It participates in the regulatory process controlling autoreactivity and therefore has been considered a strong candidate gene in autoimmune diseases. In the search for genes that might influence EPF pathogenesis, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CTLA4; Pemphigus; Fogo selvagem; Autoimmunity; Genetic susceptibility; Brazilian population.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900010

Clinical efficacy of tirofiban combined with a Solitaire stent in treating acute ischemic stroke
Provedor de dados: 56
Autores: Zhang,Yang; Zhang,Qing-Qing; Fu,Cong; Wang,Lei; Zhang,Guan-Qun; Cao,Pei-Wei; Chen,Guo-Fang; Fu,Xin-Min.
This study explores the safety and effect of acute cerebral infarction treatment by microcatheter injection of tirofiban combined with a Solitaire AB stent and/or stent implantation. Emergency cerebral angiograms showing the responsible vascular occlusion of 120 acute cerebral infarction patients who underwent emergency endovascular thrombectomy were included in the study. These patients were randomly divided into two groups using the random number table method: treatment group (n=60) that received thrombectomy (with cerebral artery stents) combined with intracerebral injection of tirofiban and control group (n=60) that only received thrombectomy (with cerebral artery stents alone). The baseline data, cerebral angiography before and after surgery,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute ischemic stroke; Solitaire AB stent; Endovascular thrombectomy; Tirofiban.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000603

Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide
Provedor de dados: 56
Autores: Ling,H.; Wen,L.; Ji,X.X.; Tang,Y.L.; He,J.; Tan,H.; Xia,H.; Zhou,J.G.; Su,Q.
Diallyl disulfide (DADS) inhibits growth and induces cell cycle G2/M arrest in human gastric cancer MGC803 cells. In this study, 15 mg/L DADS exerted similar effects on growth and cell cycle arrest in human gastric cancer BGC823 cells. Due to the importance of cell cycle redistribution in DADS-mediated anti-carcinogenic effects, we investigated the role of checkpoint kinases (Chk1 and Chk2) during DADS-induced cell cycle arrest. We hypothesized that DADS could mediate G2/M phase arrest through either Chk1 or Chk2 signal transduction pathways. We demonstrated that DADS induced the accumulation of phosphorylated Chk1, but not of Chk2, and that DADS down-regulated Cdc25C and cyclin B1. The expression of mRNA and total protein for Chkl and Chk2 was unchanged....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diallyl disulfide; Cell cycle G2/M arrest; Checkpoint kinase-1; Gastric cancer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300008

Alternative oxidase in the branched mitochondrial respiratory network: an overview on structure, function, regulation, and role
Provedor de dados: 56
Autores: Sluse,F.E.; Jarmuszkiewicz,W.
Plants and some other organisms including protists possess a complex branched respiratory network in their mitochondria. Some pathways of this network are not energy-conserving and allow sites of energy conservation to be bypassed, leading to a decrease of the energy yield in the cells. It is a challenge to understand the regulation of the partitioning of electrons between the various energy-dissipating and -conserving pathways. This review is focused on the oxidase side of the respiratory chain that presents a cyanide-resistant energy-dissipating alternative oxidase (AOX) besides the cytochrome pathway. The known structural properties of AOX are described including transmembrane topology, dimerization, and active sites. Regulation of the alternative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondria; Alternative oxidase; Structure; Regulation; Electron partitioning.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600003

Down-regulated miR-448 relieves spinal cord ischemia/reperfusion injury by up-regulating SIRT1
Provedor de dados: 56
Autores: Wang,Yun; Pang,Qing-Jiang; Liu,Jiang-Tao; Wu,Hai-Hao; Tao,Dong-Ying.
MicroRNAs play a crucial role in the progression of spinal cord ischemia/reperfusion injury (SCII). The role of miR-448 and SIRT1 in SCII was investigated in this study, to provide further insights into prevention and improvement of this disorder. In this study, expressions of miR-448 and SIRT1 protein were determined by qRT-PCR and western blot, respectively. Flow cytometry was used to analyze cell apoptosis. The endogenous expression of genes was modulated by recombinant plasmids and cell transfection. Dual-luciferase reporter assay was performed to determine the interaction between miR-448 and SIRT1. The Basso, Beattie, and Bresnahan score was used to measure the hind-limb function of rat. The spinal cord ischemia reperfusion injury model of adult rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SCII; MiR-448; SIRT1; Nerve cells.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500610

A morphometric study of the lumbar spinous process in the Chinese population
Provedor de dados: 56
Autores: Cai,B.; Ran,B.; Li,Q.; Li,Z.H.; Li,F.N.; Li,M.; Yan,W.J.
Our goal was to analyze the anatomical parameters of the lumbar spine spinous process for an interspinous stabilization device designed for the Chinese population and to offer an anatomical basis for its clinical application. The posterior lumbar spines (T12-S1) of 52 adult cadavers were used for measuring the following: distance between two adjacent spinous processes (DB), distance across two adjacent spinous processes (DA), thickness of the central spinous processes (TC), thickness of the superior margin of the spinous processes (TS), thickness of the inferior margin of the spinous processes (TI), and height of the spinous processes (H). Variance and correlation analyses were conducted for these data, and the data met the normal distribution and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lumbar spine; Morphometry; Spinal stenosis; Spinous process.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100091

Emergence of clonal complex 5 (CC5) methicillin-resistant Staphylococcus aureus (MRSA) isolates susceptible to trimethoprim-sulfamethoxazole in a Brazilian hospital
Provedor de dados: 56
Autores: Teixeira,M.M.; Araújo,M.C.; Silva-Carvalho,M.C.; Beltrame,C.O.; Oliveira,C.C.H.B.; Figueiredo,A.M.S.; Oliveira,A.G.
In this study, genotyping techniques including staphylococcal chromosomal cassette mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and restriction-modification tests were used to compare the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered at two times within a 10-year interval (1998 and 2008) from a tertiary Brazilian hospital. In addition, the antimicrobial susceptibility profiles were analyzed. All 48 MRSA isolates from 1998 and 85.7% from 2008 (48/56 isolates) displayed multidrug-resistance phenotypes and SCCmec III. All but one of the 13 representative SCCmec III isolates belonged to CC8 and had PFGE patterns similar to that of the BMB9393 strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Antimicrobial susceptibility; CC5; Cordobes clone; Trimethoprim-sulfamethoxazole.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700011

A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide
Provedor de dados: 56
Autores: Ramos,C.R.R.; Abreu,P.A.E.; Nascimento,A.L.T.O.; Ho,P.L.
We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The resulting pAE plasmid combined the advantages of both vectors: small size (pRSET), expression of a short 6XHis tag at N-terminus (pET3-His) and a high copy number of plasmid (pRSET). The small size of the vector (2.8 kb) and the high copy number/cell (200-250 copies) facilitate the subcloning and sequencing procedures when compared to the pET system (pET3-His, 4.6 kb and 40-50 copies) and also result in high level expression of recombinant proteins (20 mg purified protein/liter of culture). In addition, the vector pAE enables the expression of a fusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Expression vector; Immobilized metal affinity chromatography; Protein purification.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800001

Bi-directional actions of estrogen on the renin-angiotensin system
Provedor de dados: 56
Autores: Brosnihan,K.B.; Senanayake,P.S.; Li,P.; Ferrario,C.M.
Estrogen stimulates the renin-angiotensin system by augmenting both tissue and circulating levels of angiotensinogen and renin. We show, however, that angiotensin converting enzyme (ACE) activity in the circulation and in tissues is reduced in two animal models of postmenopausal chronic hormone replacement. We observed a reduction of ACE activity in association with a significant increase in plasma angiotensin I (Ang I) and hyperreninemia in ovariectomized monkeys treated with Premarin (conjugated equine estrogen) replacement for 30 months. Plasma angiotensin II (Ang II) levels were not increased in monkeys treated with estrogen, suggesting that the decrease in ACE curtailed the formation of the peptide. The Ang II/Ang I ratio, an in vivo index of ACE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin; Angiotensin converting enzyme; Angiotensin peptides; Angiotensin receptors; Bradykinin; Nitric oxide; Kinins; Vasodilation; Hormone replacement.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400001

Disseminated Langerhans' cell histiocytosis and massive protein-losing enteropathy
Provedor de dados: 56
Autores: Santos-Machado,T.M.; Cristófani,L.M.; Almeida,M.T.A.; Maluf,P.T.; Costa,P.A.; Pereira,M.A.; Brito,J.L.B.C.; Odone-Filho,V.
Symptomatic involvement of the gastrointestinal (GI) tract as a prominent symptom in Langerhans' cell histiocytosis (LCH) is uncommon, occurring in less than 1 to 5% of all cases, even when the disease is in its disseminated form. Up to now, there have been reports of 18 cases of LCH with GI manifestations, including our 2 cases, with diarrhea (77.7%), protein-losing enteropathy (33.3%) and bloody stool being the most frequent findings. The authors present two patients with severe diarrhea and refractory hypoalbuminemia, and with the protein-losing enteropathy documented by Cr51-labeled albumin studies. A review of the literature indicated that the presence of GI symptoms is often associated with systemic disease as well as with poor prognosis, mainly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Langerhans' cell histiocytosis; Histiocytosis X; Gastrointestinal involvement; Protein-losing enteropathy; Hypoalbuminemia; Cr51-labeled albumin test.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900007

The modest but growing Brazilian presence in psychiatric, psychobiological and mental health research: assessment of the 1998-2002 period
Provedor de dados: 56
Autores: Bressan,R.A.; Gerolin,J.; Mari,J.J.
The objective of the present survey was to assess the Brazilian scientific production in psychiatry, psychobiology, and mental health during the 1998-2002 period. The universities' graduate programs concentrate the vast majority of the scientific production in Brazil. We assessed the annual reports from the graduate programs to the Brazilian Ministry of Education concerning master's and doctoral theses and the articles published in journals indexed by the Institute of Scientific Information (ISI). There are nine Master's and Doctoral graduate programs dedicated to research in psychiatry, neuropsychiatry, psychobiology, and mental health in the country, seven being located in southern states. During the 5-year period, from 1998 to 2002, 186 students...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mental health; Scientometrics; Bibliometric analysis; MSc and PhD students; Publish or perish; Public and private universities.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500001

Kaurene diterpene induces apoptosis in U87 human malignant glioblastoma cells by suppression of anti-apoptotic signals and activation of cysteine proteases
Provedor de dados: 56
Autores: Lizarte Neto,F.S.; Tirapelli,D.P.C.; Ambrosio,S.R.; Tirapelli,C.R.; Oliveira,F.M.; Novais,P.C.; Peria,F.M.; Oliveira,H.F.; Carlotti Junior,C.G.; Tirapelli,L.F.
Gliomas are the most common and malignant primary brain tumors in humans. Studies have shown that classes of kaurene diterpene have anti-tumor activity related to their ability to induce apoptosis. We investigated the response of the human glioblastoma cell line U87 to treatment with ent-kaur-16-en-19-oic acid (kaurenoic acid, KA). We analyzed cell survival and the induction of apoptosis using flow cytometry and annexin V staining. Additionally, the expression of anti-apoptotic (c-FLIP and miR-21) and apoptotic (Fas, caspase-3 and caspase-8) genes was analyzed by relative quantification (real-time PCR) of mRNA levels in U87 cells that were either untreated or treated with KA (30, 50, or 70 µM) for 24, 48, and 72 h. U87 cells treated with KA demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kaurenoic acid; Glioblastoma; MiR-21; C-FLIP; Apoptosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100071

Nitrergic modulation of vasopressin, oxytocin and atrial natriuretic peptide secretion in response to sodium intake and hypertonic blood volume expansion
Provedor de dados: 56
Autores: Ventura,R.R.; Gomes,D.A.; Reis,W.L.; Elias,L.L.K.; Castro,M.; Valença,M.M.; Carnio,E.C.; Rettori,V.; McCann,S.M.; Antunes-Rodrigues,J.
The central nervous system plays an important role in the control of renal sodium excretion. We present here a brief review of physiologic regulation of hydromineral balance and discuss recent results from our laboratory that focus on the participation of nitrergic, vasopressinergic, and oxytocinergic systems in the regulation of water and sodium excretion under different salt intake and hypertonic blood volume expansion (BVE) conditions. High sodium intake induced a significant increase in nitric oxide synthase (NOS) activity in the medial basal hypothalamus and neural lobe, while a low sodium diet decreased NOS activity in the neural lobe, suggesting that central NOS is involved in the control of sodium balance. An increase in plasma concentrations in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasopressin; Oxytocin; ANP; Nitric oxide; Blood volume expansion; Sodium diet.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900011

A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats
Provedor de dados: 56
Autores: Fernandes,A.A.; Faria,T. de O.; Ribeiro Júnior,R.F.; Costa,G.P.; Marchezini,B.; Silveira,E.A.; Angeli,J.K.; Stefanon,I.; Vassallo,D.V.; Lizardo,J.H.
Resistance training evokes myocardial adaptation; however, the effects of a single resistance exercise session on cardiac performance are poorly understood or investigated. This study aimed to investigate the effects of a single resistance exercise session on the myocardial contractility of spontaneously hypertensive rats (SHRs). Male 3-month-old SHRs were divided into two groups: control (Ct) and exercise (Ex). Control animals were submitted to sham exercise. Blood pressure was measured in conscious rats before the exercise session to confirm the presence of arterial hypertension. Ten minutes after the exercise session, the animals were anesthetized and killed, and the hearts were removed. Cardiac contractility was evaluated in the whole heart by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac; Contractility; Myocardium; Proteins; Resistance exercise.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900813

Ethological analysis of mother-pup interactions and other behavioral reactions in rats: effects of malnutrition and tactile stimulation of the pups
Provedor de dados: 56
Autores: Riul,T.R.; Carvalho,A.F.; Almeida,P.S.; De-Oliveira,L.M.; Almeida,S.S.
Mother-pup interaction, as well as other behavioral reactions were studied during the lactation period in 24 litters of Wistar rats and their dams fed either a 16% (control - C; 12 litters) or a 6% (malnourished - M; 12 litters) protein diet. The diets were isocaloric. Throughout lactation there was a 36.4% weight loss of M dams and a 63% body weight deficit in the M pups when compared to control pups. During this period, half of the litters were exposed daily to additional tactile stimulation (CS or MS), while the other half were submitted to normal rearing conditions (CN or MN). The tactile stimulation of pups (handling) consisted of holding the animal in one hand and gently touching the dorsal part of the animal's body with the fingers for 3 min. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein malnutrition; Tactile stimulation; Mother-pup interaction.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800007

Comparison of scientists of the Brazilian Academy of Sciences and of the National Academy of Sciences of the USA on the basis of the h-index
Provedor de dados: 56
Autores: Mugnaini,R.; Packer,A.L.; Meneghini,R.
A new scientometric indicator, the h-index, has been recently proposed (Hirsch JE. Proc Natl Acad Sci 2005; 102: 16569-16572). The index avoids some shortcomings of the calculation of the total number of citations as a parameter to evaluate scientific performance. Although it has become known only recently, it has had widespread acceptance. A comparison of the average h-index of members of the Brazilian Academy of Sciences (BAS) and of the National Academy of Sciences of the USA (NAS-USA) was carried out for 10 different areas of science. Although, as expected, the comparison was unfavorable to the members of the BAS, the imbalance was distinct in different areas. Since these two academies represent, to a significant extent, the science of top quality...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Scientometrics; Bibliometrics; Index h; Science evaluation; Scientific academy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400001

Estimating the average length of hospitalization due to pneumonia: a fuzzy approach
Provedor de dados: 56
Autores: Nascimento,L.F.C.; Rizol,P.M.S.R.; Peneluppi,A.P.
Exposure to air pollutants is associated with hospitalizations due to pneumonia in children. We hypothesized the length of hospitalization due to pneumonia may be dependent on air pollutant concentrations. Therefore, we built a computational model using fuzzy logic tools to predict the mean time of hospitalization due to pneumonia in children living in São José dos Campos, SP, Brazil. The model was built with four inputs related to pollutant concentrations and effective temperature, and the output was related to the mean length of hospitalization. Each input had two membership functions and the output had four membership functions, generating 16 rules. The model was validated against real data, and a receiver operating characteristic (ROC) curve was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollutants; Fuzzy logic; Pneumonia; Particulate matter; Sulfur dioxide.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100977

Efficient retrovirus-mediated transfer of cell-cycle control genes to transformed cells
Provedor de dados: 56
Autores: Strauss,B.E.; Costanzi-Strauss,E.
The use of gene therapy continues to be a promising, yet elusive, alternative for the treatment of cancer. The origins of cancer must be well understood so that the therapeutic gene can be chosen with the highest chance of successful tumor regression. The gene delivery system must be tailored for optimum transfer of the therapeutic gene to the target tissue. In order to accomplish this, we study models of G1 cell-cycle control in both normal and transformed cells in order to understand the reasons for uncontrolled cellular proliferation. We then use this information to choose the gene to be delivered to the cells. We have chosen to study p16, p21, p53 and pRb gene transfer using the pCL-retrovirus. Described here are some general concepts and specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene therapy; Clinical trial; Retrovirus; Animal model; Cell cycle; Growth arrest.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700016

Epidemiological studies in the information and genomics era: experience of the Clinical Genome of Cancer Project in São Paulo, Brazil
Provedor de dados: 56
Autores: Wünsch-Filho,V.; Eluf-Neto,J.; Lotufo,P.A.; da Silva Jr.,W.A.; Zago,M.A.
Genomics is expanding the horizons of epidemiology, providing a new dimension for classical epidemiological studies and inspiring the development of large-scale multicenter studies with the statistical power necessary for the assessment of gene-gene and gene-environment interactions in cancer etiology and prognosis. This paper describes the methodology of the Clinical Genome of Cancer Project in São Paulo, Brazil (CGCP), which includes patients with nine types of tumors and controls. Three major epidemiological designs were used to reach specific objectives: cross-sectional studies to examine gene expression, case-control studies to evaluate etiological factors, and follow-up studies to analyze genetic profiles in prognosis. The clinical groups included...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multicenter studies; Large-scale studies; Molecular epidemiology; Data control quality; Cancer epidemiological studies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400016

Pancreatic nitric oxide and oxygen free radicals in the early stages of streptozotocin-induced diabetes mellitus in the rat
Provedor de dados: 56
Autores: González,E.; Roselló-Catafau,J.; Jawerbaum,A.; Sinner,D.; Pustovrh,C.; Vela,J.; White,V.; Xaus,C.; Peralta,C.; Gimeno,M.
The objective of the present study was to explore the regulatory mechanisms of free radicals during streptozotocin (STZ)-induced pancreatic damage, which may involve nitric oxide (NO) production as a modulator of cellular oxidative stress. Removal of oxygen species by incubating pancreatic tissues in the presence of polyethylene glycol-conjugated superoxide dismutase (PEG-SOD) (1 U/ml) produced a decrease in nitrite levels (42%) and NO synthase (NOS) activity (50%) in diabetic but not in control samples. When NO production was blocked by N G-monomethyl-L-arginine (L-NMMA) (600 µM), SOD activity increased (15.21 ± 1.23 vs 24.40 ± 2.01 U/mg dry weight). The increase was abolished when the NO donor, spermine nonoate, was added to the incubating medium (13.2 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptozotocin diabetes; Pancreas; Oxidative stress; Nitric oxide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100012

Brazilian medical publications: citation patterns for Brazilian-edited and non-Brazilian literature
Provedor de dados: 56
Autores: Cunha-Melo,J.R.; Santos,G.C.; Andrade,M.V.
Today, the quality of a scientific article depends on the periodical in which it is published and on the number of times the article is cited in the literature. In Brazil, the criteria for the evaluation of this scientific production are improving. However, there is still some resistance, with authors arguing that Brazilian publications must be preferentially addressed to the national readers and, therefore, they should ideally be written in Portuguese. In order to determine the kind of scientific journals cited in the reference lists of articles published in medical periodicals edited in Brazil, in the present study we determine the rate of Portuguese/English citations. Three issues of 43 periodicals (19 indexed in SciELO, 10 in PubMed, 10 in LILACS, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Brazilian medical journals; Bibliometrics; Citation patterns; Brazilian scientific journals.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800001

Plasma metanephrines: a novel and cost-effective test for pheochromocytoma
Provedor de dados: 56
Autores: Eisenhofer,G.; Walther,M.; Keiser,H.R.; Lenders,J.W.M.; Friberg,P.; Pacak,K.
Pheochromocytomas are rare chromaffin cell tumors that nevertheless must be excluded in large numbers of patients who develop sustained or episodic hypertension as well as in many others with suggestive symptoms or with a familial history of pheochromocytoma. Diagnosis of pheochromocytoma depends importantly on biochemical evidence of excess catecholamine production by a tumor. Imperfect sensitivity and specificity of commonly available biochemical tests and the low incidence of the tumor among the tested population mean that considerable time and effort can be expended in confirming or ruling out pheochromocytoma in patients where the tumor is suspected. Measurements of plasma free metanephrines provide a superior test compared to other available tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pheochromocytoma; Hypertension; Sympathetic nervous system; Adrenal gland; Metanephrines; Catecholamines.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000005

Atherosclerosis in aged mice over-expressing the reverse cholesterol transport genes
Provedor de dados: 56
Autores: Berti,J.A.; de Faria,E.C.; Oliveira,H.C.F.
We determined whether over-expression of one of the three genes involved in reverse cholesterol transport, apolipoprotein (apo) AI, lecithin-cholesterol acyl transferase (LCAT) and cholesteryl ester transfer protein (CETP), or of their combinations influenced the development of diet-induced atherosclerosis. Eight genotypic groups of mice were studied (AI, LCAT, CETP, LCAT/AI, CETP/AI, LCAT/CETP, LCAT/AI/CETP, and non-transgenic) after four months on an atherogenic diet. The extent of atherosclerosis was assessed by morphometric analysis of lipid-stained areas in the aortic roots. The relative influence (R²) of genotype, sex, total cholesterol, and its main sub-fraction levels on atherosclerotic lesion size was determined by multiple linear regression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein AI; Cholesteryl ester transfer protein; Lecithin-cholesterol acyl transferase; Atherosclerosis; Transgenic mice.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300010

Role of mitochondrial ATP-sensitive potassium channel-mediated PKC-ε in delayed protection against myocardial ischemia/reperfusion injury in isolated hearts of sevoflurane-preconditioned rats
Provedor de dados: 56
Autores: Wang,C.; Hu,S.M.; Xie,H.; Qiao,S.G.; Liu,H.; Liu,C.F.
This study aimed to determine the role of mitochondrial adenosine triphosphate-sensitive potassium (mitoKATP) channels and protein kinase C (PKC)-ε in the delayed protective effects of sevoflurane preconditioning using Langendorff isolated heart perfusion models. Fifty-four isolated perfused rat hearts were randomly divided into 6 groups (n=9). The rats were exposed for 60 min to 2.5% sevoflurane (the second window of protection group, SWOP group) or 33% oxygen inhalation (I/R group) 24 h before coronary occlusion. The control group (CON) and the sevoflurane group (SEVO) group were exposed to 33% oxygen and 2.5% sevoflurane for 60 min, respectively, without coronary occlusion. The mitoKATP channel inhibitor 5-hydroxydecanoate (5-HD) was given 30 min before...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemia; Reperfusion; MitoKatp channel; Preconditioning; Protein kinase C.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600528

Neuroprotection by flavonoids
Provedor de dados: 56
Autores: Dajas,F.; Rivera-Megret,F.; Blasina,F.; Arredondo,F.; Abin-Carriquiry,J.A.; Costa,G.; Echeverry,C.; Lafon,L.; Heizen,H.; Ferreira,M.; Morquio,A.
The high morbidity, high socioeconomic costs and lack of specific treatments are key factors that define the relevance of brain pathology for human health and the importance of research on neuronal protective agents. Epidemiological studies have shown beneficial effects of flavonoids on arteriosclerosis-related pathology in general and neurodegeneration in particular. Flavonoids can protect the brain by their ability to modulate intracellular signals promoting cellular survival. Quercetin and structurally related flavonoids (myricetin, fisetin, luteolin) showed a marked cytoprotective capacity in in vitro experimental conditions in models of predominantly apoptotic death such as that induced by medium concentrations (200 µM) of H2O2 added to PC12 cells in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavonoids; Quercetin; Focal ischemia; Neuroprotection.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200002

Effects of serotonin and fluoxetine on blood glucose regulation in two decapod species
Provedor de dados: 56
Autores: Santos,E.A.; Keller,R.; Rodriguez,E.; Lopez,L.
One of the best known crustacean hormones is the crustacean hyperglycemic hormone (CHH). However, the mechanisms involved in hormone release in these animals are poorly understood, and thus constitute the central objective of the present study. Different groups of crustaceans belonging to diverse taxa (Chasmagnathus granulata, a grapsid crab and Orconectes limosus, an astacid) were injected with serotonin, fluoxetine, or a mixture of both, and glycemic values (C. granulata and O. limosus) and CHH levels (O. limosus) were determined after 2 h in either submerged animals or animals exposed to atmospheric air. Both serotonin and fluoxetine caused significant hyperglycemia (P&lt;0.05) after injection into the blood sinus of the two species, an effect enhanced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacean; Reproduction; Endocrinology; CHH; Crab; Crayfish.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100009

Antinociception synergy between the peripheral and spinal sites of the heme oxygenase-carbon monoxide pathway
Provedor de dados: 56
Autores: Nascimento,C.G.O.; Branco,L.G.S.
We have shown that the peripheral and spinal cord heme oxygenase (HO)-carbon monoxide (CO)-soluble guanylate cyclase-cGMP pathways play an important role in antinociception in the rat experimental formalin model. Our objective was to determine if there is synergism between peripheral (paw) and spinal HO-CO pathways in nociception. Rats were handled and adapted to the experimental environment for a few days before the formalin test, in which 50 µL of a 1% formalin was injected subcutaneously into the dorsal surface of the right hind paw. The animals were then observed for 1 h and the frequency of flinching behavior was taken to represent the nociceptive response. Thirty minutes before the test, rats were pretreated with intrathecal injections of the HO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nociception; Carbon monoxide; Formalin; Hypernociception; Antinociception.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100020

Antigenic stimulation is more efficient than LPS in inducing nitric oxide production by human mononuclear cells on the in vitro granuloma reaction in schistosomiasis
Provedor de dados: 56
Autores: Oliveira,D.M.; Silva-Teixeira,D.N.; Araújo-Filho,R.; Goes,A.M.
Nitric oxide (NO) is an extremely important and versatile messenger in biological systems. It has been identified as a cytotoxic factor in the immune system, presenting anti- or pro-inflammatory properties under different circumstances. In murine monocytes and macrophages, stimuli by cytokines or lipopolysaccharide (LPS) are necessary for inducing the immunologic isoform of the enzyme responsible for the high-output production of NO, nitric oxide synthase (iNOS). With respect to human cells, however, LPS seems not to stimulate NO production in the same way. Addressing this issue, we demonstrate here that peripheral blood mononuclear cells (PBMC) obtained from schistosomiasis-infected patients and cultivated with parasite antigens in the in vitro granuloma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; LPS; Schistosomiasis infection; In vitro granuloma.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100015

Radioiodine plus recombinant human thyrotropin do not cause acute airway compression and are effective in reducing multinodular goiter
Provedor de dados: 56
Autores: Albino,C.C.; Graf,H.; Paz-Filho,G.; Diehl,L.A.; Olandoski,M.; Sabbag,A.; Buchpiguel,C.
Recombinant human thyrotropin (rhTSH) reduces the activity of radioiodine required to treat multinodular goiter (MNG), but acute airway compression can be a life-threatening complication. In this prospective, randomized, double-blind, placebo-controlled study, we assessed the efficacy and safety (including airway compression) of different doses of rhTSH associated with a fixed activity of 131I for treating MNG. Euthyroid patients with MNG (69.3 ± 62.0 mL, 20 females, 2 males, 64 ± 7 years) received 0.1 mg (group I, N = 8) or 0.01 mg (group II, N = 6) rhTSH or placebo (group III, N = 8), 24 h before 1.11 GBq 131I. Radioactive iodine uptake was determined at baseline and 24 h after rhTSH and thyroid volume (TV, baseline and 6 and 12 months after treatment)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperthyroidism; Multinodular goiter; Tracheal airway; 131I; Thyrotropin alpha.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300013

Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy
Provedor de dados: 56
Autores: Vesentini,G.; Marini,G.; Piculo,F.; Damasceno,D.C.; Matheus,S.M.M.; Felisbino,S.L.; Calderon,I.M.P.; Hijaz,A.; Barbosa,A.M.P.; Rudge,M.V.C.
The urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Pregnancy; Collagen; Skeletal muscle; Rats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400611

Clinical trial of gabexate in the prophylaxis of post-endoscopic retrograde cholangiopancreatography pancreatitis
Provedor de dados: 56
Autores: Xiong,G.-S.; Wu,S.-M.; Zhang,X.-W.; Ge,Z.-Z.
The objective of the present study was to determine the efficacy of prophylactic administration of gabexate for the prevention of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis, hyperamylasemia and pancreatic pain. Patients scheduled for ERCP were randomized into two groups in a double-blind manner: the patients in the gabexate group were treated with continuous intravenous infusion of 300 mg gabexate dissolved in 500 mL Ringer's solution at 111 mL/h, starting 30 min before the endoscopic maneuvers and continuing up to 4 h after them; placebo group patients were treated only with Ringer's solution also starting 30 min before the endoscopic maneuvers and continuing up to 4 h. Data for 193 patients were analyzed. The incidence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gabexate; Endoscopic retrograde cholangiopancreatography; Post-ERCP pancreatitis; Prevention of pancreatitis; Hyperamylasemia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100010

Training-related changes in the R-R interval at the onset of passive movements in humans
Provedor de dados: 56
Autores: Vianna,L.C.; Ricardo,D.R.; Araújo,C.G.S.
The aim of the present study was to determine whether training-related alterations in muscle mechanoreflex activation affect cardiac vagal withdrawal at the onset of exercise. Eighteen male volunteers divided into 9 controls (26 ± 1.9 years) and 9 racket players (25 ± 1.9 years) performed 10 s of voluntary and passive movement characterized by the wrist flexion of their dominant and non-dominant limbs. The respiratory cycle was divided into four phases and the phase 4 R-R interval was measured before and immediately following the initiation of either voluntary or passive movement. At the onset of voluntary exercise, the decrease in R-R interval was similar between dominant and non-dominant forearms in both controls (166 ± 20 vs 180 ± 34 ms, respectively; P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mechanoreceptors; Vagus nerve; Muscle stretching exercises; R-R interval.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900014

Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis
Provedor de dados: 56
Autores: Sato,N.S.; Suzuki,T.; Ueda,T.; Watanabe,K.; Hirata,R.D.C.; Hirata,M.H.
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and GST-rTp15, respectively. The identities of the antigens obtained were confirmed by automated DNA sequencing using ABI Prism 310 and peptide mapping by Finningan LC/MS. These recombinant antigens were evaluated by immuno-slot blot techniques applied to 137 serum samples from patients with a clinical and laboratory diagnosis of syphilis (61 samples), from healthy...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Treponema pallidum; Cloning; Recombinant antigen; Syphilis; GST.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700002

Statistical analysis of event-related potential elicited by verb-complement merge in Brazilian Portuguese
Provedor de dados: 56
Autores: Cagy,M.; Infantosi,A.F.C.; França,A.I.; Lemle,M.
An interesting fact about language cognition is that stimulation involving incongruence in the merge operation between verb and complement has often been related to a negative event-related potential (ERP) of augmented amplitude and latency of ca. 400 ms - the N400. Using an automatic ERP latency and amplitude estimator to facilitate the recognition of waves with a low signal-to-noise ratio, the objective of the present study was to study the N400 statistically in 24 volunteers. Stimulation consisted of 80 experimental sentences (40 congruous and 40 incongruous), generated in Brazilian Portuguese, involving two distinct local verb-argument combinations (nominal object and pronominal object series). For each volunteer, the EEG was simultaneously acquired at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Event-related brain potentials; Verb-complement merges; Averaging; N400 identification.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100010

Compounds used to produce cloned animals are genotoxic and mutagenic in mammalian assays in vitro and in vivo
Provedor de dados: 56
Autores: Oliveira,R.J.; Mantovani,M.S.; da Silva,A.F.; Pesarini,J.R.; Mauro,M.O.; Ribeiro,L.R.
The compounds 6-dimethylaminopurine and cycloheximide promote the successful production of cloned mammals and have been used in the development of embryos produced by somatic cell nuclear transfer. This study investigated the effects of 6-dimethylaminopurine and cycloheximide in vitro, using the thiazolyl blue tetrazolium bromide colorimetric assay to assess cytotoxicity, the trypan blue exclusion assay to assess cell viability, the comet assay to assess genotoxicity, and the micronucleus test with cytokinesis block to test mutagenicity. In addition, the comet assay and the micronucleus test were also performed on peripheral blood cells of 54 male Swiss mice, 35 g each, to assess the effects of the compounds in vivo. The results indicated that both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic cell nuclear transfer; 6-Dimethylaminopurine; Cycloheximide; Genotoxic; Mutagenic.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400287

Effect of epithelial debridement on human cornea proteoglycans
Provedor de dados: 56
Autores: Soriano,E.S.; Campos,M.S.Q.; Aguiar,J.A.K.; Michelacci,Y.M.
Corneal transparency is attributed to the regular spacing and diameter of collagen fibrils, and proteoglycans may play a role in fibrillogenesis and matrix assembly. Corneal scar tissue is opaque and this opacity is explained by decreased ultrastructural order that may be related to proteoglycan composition. Thus, the objectives of the present study were to characterize the proteoglycans synthesized by human corneal explants and to investigate the effect of mechanical epithelial debridement. Human corneas unsuitable for transplants were immersed in F-12 culture medium and maintained under tissue culture conditions. The proteoglycans synthesized in 24 h were labeled metabolically by the addition of 35S-sulfate to the medium. These compounds were extracted...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Corneal explants; De-epithelization; Glycosaminoglycan; Proteoglycan.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300005

Development of the insulin secretion mechanism in fetal and neonatal rat pancreatic B-cells: response to glucose, K+, theophylline, and carbamylcholine
Provedor de dados: 56
Autores: Mendonça,A.C.; Carneiro,E.M.; Bosqueiro,J.R.; Crepaldi-Alves,S.C.; Boschero,A.C.
We studied the development of the insulin secretion mechanism in the pancreas of fetal (19- and 21-day-old), neonatal (3-day-old), and adult (90-day-old) rats in response to stimulation with 8.3 or 16.7 mM glucose, 30 mM K+, 5 mM theophylline (Theo) and 200 µM carbamylcholine (Cch). No effect of glucose or high K+ was observed on the pancreas from 19-day-old fetuses, whereas Theo and Cch significantly increased insulin secretion at this age (82 and 127% above basal levels, respectively). High K+ also failed to alter the insulin secretion in the pancreas from 21-day-old fetuses, whereas 8.3 mM and 16.7 mM glucose significantly stimulated insulin release by 41 and 54% above basal levels, respectively. Similar results were obtained with Theo and Cch. A more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat islet cell; Glucose; Pancreatic islets; Carbamylcholine; Theophylline; Potassium; Insulin secretion.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600018

Bone marrow mesenchymal stem cells overexpressing human basic fibroblast growth factor increase vasculogenesis in ischemic rats
Provedor de dados: 56
Autores: Zhang,J.C.; Zheng,G.F.; Wu,L.; Ou Yang,L.Y.; Li,W.X.
Administration or expression of growth factors, as well as implantation of autologous bone marrow cells, promote in vivo angiogenesis. This study investigated the angiogenic potential of combining both approaches through the allogenic transplantation of bone marrow-derived mesenchymal stem cells (MSCs) expressing human basic fibroblast growth factor (hbFGF). After establishing a hind limb ischemia model in Sprague Dawley rats, the animals were randomly divided into four treatment groups: MSCs expressing green fluorescent protein (GFP-MSC), MSCs expressing hbFGF (hbFGF-MSC), MSC controls, and phosphate-buffered saline (PBS) controls. After 2 weeks, MSC survival and differentiation, hbFGF and vascular endothelial growth factor (VEGF) expression, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiogenesis; Basic fibroblast growth factor; Bone marrow mesenchymal stem cells; Gene transfection; Ischemia.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000886

Demographic and metabolic characteristics of individuals with progressive glucose tolerance
Provedor de dados: 56
Autores: Mendes,A.L.; Santos,M.L.; Padovani,C.R.; Pimenta,W.P.
We evaluated changes in glucose tolerance of 17 progressors and 62 non-progressors for 9 years to improve our understanding of the pathogenesis of type 2 diabetes mellitus. Changes in anthropometric measurements and responses to an oral glucose tolerance test (OGTT) were analyzed. We identified 14 pairs of individuals, one from each group, who were initially normal glucose tolerant and were matched for gender, age, weight, and girth. We compared initial plasma glucose and insulin curves (from OGTT), insulin secretion (first and second phases) and insulin sensitivity indices (from hyperglycemic clamp assay) for both groups. In the normal glucose tolerant phase, progressors presented: 1) a higher OGTT blood glucose response with hyperglycemia in the second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Impaired glucose tolerance; Impaired insulin secretion; Normal glucose tolerance; Hyperglycemic clamp; Oral glucose tolerance test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300009

Synergistic effect of mycophenolate mofetil and angiotensin-converting enzyme inhibitor in patients with chronic allograft nephropathy
Provedor de dados: 56
Autores: Moscoso-Solorzano,G.T.; Mastroianni-Kirsztajn,G.; Ozaki,K.S.; Franco,M.F.; Pacheco-Silva,A.; Câmara,N.O.S.
Experimental data and few clinical non-randomized studies have shown that inhibition of the renin-angiotensin system by angiotensin-converting enzyme (ACE) associated or not with the use of mycophenolate mofetil (MMF) could delay or even halt the progression of chronic allograft nephropathy (CAN). In this retrospective historical study, we investigated whether ACE inhibition (ACEI) associated or not with the use of MMF has the same effect in humans as in experimental studies and what factors are associated with a clinical response. A total of 160 transplant patients with biopsy-proven CAN were enrolled. Eighty-one of them were on ACE therapy (G1) and 80 on ACEI_free therapy (G2). Patients were further stratified for the use of MMF. G1 patients showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Mycophenolate mofetil; Renal allograft survival; Kidney transplantation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500008

Effect of strontium ranelate on pain behavior in an experimental model of osteoarthritis
Provedor de dados: 56
Autores: Rodrigues,T.A.; Sampaio Junior,A.J.B.; Nunes,I.D.P.; Cartágenes,M.S.S.; Garcia,J.B.S.
Strontium ranelate (SrRan) is a drug usually prescribed to treat osteoporosis, with proven effects of decreasing the risk of fractures and an indication of reducing the progression of osteoarthritis (OA). This study aimed to investigate the effects of SrRan as either a prophylactic or a treatment drug, using an OA rat model to assess pain behavior. A monoiodoacetate (MIA)-induced knee joint OA model in Wistar rats was used. Thirty Wistar rats (both sexes, 60 days old) were distributed in five groups of 6 rats each: the control group, that received no intervention; a prophylactic group, that received oral administration of 25 mg·kg-1·day-1 of SrRan for 28 days before induction of OA; a group treated with 25 mg·kg-1·day-1 of SrRan for 28 days after OA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoarthritis; Strontium ranelate; Pain; Treatment; Prophylaxis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900602

Long-lasting mnemotropic effect of substance P and its N-terminal fragment (SP1-7) on avoidance learning
Provedor de dados: 56
We investigated the long-lasting effect of peripheral injection of the neuropeptide substance P (SP) and of some N- or C-terminal SP fragments (SPN and SPC, respectively) on retention test performance of avoidance learning. Male Wistar rats (220 to 280 g) were trained in an inhibitory step-down avoidance task and tested 24 h or 21 days later. Immediately after the training trial rats received an intraperitoneal injection of SP (50 µg/kg), SPN 1-7 (167 µg/kg) or SPC 7-11 (134 µg/kg). Control groups were injected with vehicle or SP 5 h after the training trial. The immediate post-training administration of SP and SPN, but not SPC, facilitated avoidance behavior in rats tested 24 h or 21 days later, i.e., the retention test latencies of the SP and SPN groups...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Substance P; Substance P fragments; Avoidance conditioning; Long-lasting memory.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200011

Polymorphisms of the low-density lipoprotein receptor gene in Brazilian individuals with heterozygous familial hypercholesterolemia
Provedor de dados: 56
Autores: Salazar,L.A.; Cavalli,S.A.; Hirata,M.H.; Diament,J.; Forti,N.; Giannini,S.D.; Nakandakare,E.R.; Bertolami,M.C.; Hirata,R.D.C.
Familial hypercholesterolemia (FH) is a metabolic disorder inherited as an autosomal dominant trait characterized by an increased plasma low-density lipoprotein (LDL) level. The disease is caused by several different mutations in the LDL receptor gene. Although early identification of individuals carrying the defective gene could be useful in reducing the risk of atherosclerosis and myocardial infarction, the techniques available for determining the number of the functional LDL receptor molecules are difficult to carry out and expensive. Polymorphisms associated with this gene may be used for unequivocal diagnosis of FH in several populations. The aim of our study was to evaluate the genotype distribution and relative allele frequencies of three...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Familial hypercholesterolemia; DNA polymorphism; Atherosclerosis; Genetics; LDL receptor.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100006

Partial baroreceptor dysfunction and low plasma nitric oxide bioavailability as determinants of salt-sensitive hypertension: a reverse translational rat study
Provedor de dados: 56
Autores: Rodríguez-Pérez,A.S.; López-Rodríguez,J.F.; Calvo-Turrubiartes,M.Z.; Saavedra-Alanís,V.M.; Llamazares-Azuara,L.; Rodríguez-Martínez,M.
This study determined whether clinical salt-sensitive hypertension (cSSHT) results from the interaction between partial arterial baroreceptor impairment and a high-sodium (HNa) diet. In three series (S-I, S-II, S-III), mean arterial pressure (MAP) of conscious male Wistar ChR003 rats was measured once before (pdMAP) and twice after either sham (SHM) or bilateral aortic denervation (AD), following 7 days on a low-sodium (LNa) diet (LNaMAP) and then 21 days on a HNa diet (HNaMAP). The roles of plasma nitric oxide bioavailability (pNOB), renal medullary superoxide anion production (RMSAP), and mRNA expression of NAD(P)H oxidase and superoxide dismutase were also assessed. In SHM (n=11) and AD (n=15) groups of S-I, LNaMAP-pdMAP was 10.5±2.1 vs 23±2.1 mmHg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salt-sensitive hypertension; Aortic barodenervation; Nitric oxide bioavailability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000868

The role of IL-12 in experimental Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Silva,J.S.; Aliberti,J.C.S.; Martins,G.A.; Souza,M.A.; Souto,J.T.; Pádua,M.A.
Host resistance to Trypanosoma cruzi infection is dependent on both natural and acquired immune responses. During the early acute phase of infection in mice, natural killer (NK) cell-derived IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is involved in controlling intracellular parasite replication, mainly through the induction of nitric oxide biosynthesis by activated macrophages. We have shown that IL-12, a powerful inducer of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production by NK cells, is synthesized soon after trypomastigote-macrophage interaction. The role of IL-12 in the control of T. cruzi infection in vivo was determined by treating infected mice with anti-IL-12 monoclonal antibody (mAb) and analyzing both parasitemia and mortality during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-12 Trypanosoma cruzi NK cells IFN-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100014

Somaclonal variation: a morphogenetic and biochemical analysis of Mandevilla velutina cultured cells
Provedor de dados: 56
Autores: Maraschin,M.; Sugui,J.A.; Wood,K.V.; Bonham,C.; Buchi,D.F.; Cantao,M.P.; Carobrez,S.G.; Araujo,P.S.; Peixoto,M.L.; Verpoorte,R.; Fontana,J.D.
Cell cultures of Mandevilla velutina have proved to be an interesting production system for biomass and secondary metabolites able to inhibit the hypotensive activity of bradykinin, a nonapeptide generated in plasma during tissue trauma. The crude ethyl acetate extract of cultured cells contains about 31- to 79-fold more potent anti-bradykinin compounds (e.g., velutinol A) than that obtained with equivalent extracts of tubers. Somaclonal variation may be an explanation for the wide range of inhibitor activity found in the cell cultures. The heterogeneity concerning morphology, differentiation, carbon dissimilation, and velutinol A production in M. velutina cell cultures is reported. Cell cultures showed an asynchronous growth and cells in distinct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mandevilla velutina; Secondary metabolism; Plant cell cultures; Somaclonal variation; Phytosteroid; Anti-bradykinin compounds.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600002

In vivo photorelease of GABA in the mouse cortex
Provedor de dados: 56
Autores: Lopes-dos-Santos,V.; Campi,J.; Filevich,O.; Ribeiro,S.; Etchenique,R.
Electrical stimulation has been used for more than 100 years in neuroscientific and biomedical research as a powerful tool for controlled perturbations of neural activity. Despite quickly driving neuronal activity, this technique presents some important limitations, such as the impossibility to activate or deactivate specific neuronal populations within a single stimulation site. This problem can be avoided by pharmacological methods based on the administration of receptor ligands able to cause specific changes in neuronal activity. However, intracerebral injections of neuroactive molecules inherently confound the dynamics of drug diffusion with receptor activation. Caged compounds have been proposed to circumvent this problem, for spatially and temporally...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caged compounds; Neural stimulation; GABA; Cerebral cortex; Photorelease.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700012

Amino acid composition of parturient plasma, the intervillous space of the placenta and the umbilical vein of term newborn infants
Provedor de dados: 56
Autores: Camelo Jr.,J.S.; Jorge,S.M.; Martinez,F.E.
The objective of the present study was to determine the levels of amino acids in maternal plasma, placental intervillous space and fetal umbilical vein in order to identify the similarities and differences in amino acid levels in these compartments of 15 term newborns from normal pregnancies and deliveries. All amino acids, except tryptophan, were present in at least 186% higher concentrations in the intervillous space than in maternal venous blood, with the difference being statistically significant. This result contradicted the initial hypothesis of the study that the plasma amino acid levels in the placental intervillous space should be similar to those of maternal plasma. When the maternal venous compartment was compared with the umbilical vein, we...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fetal nutrition; Amino acid analyses; Placental intervillous space; Fetal umbilical vein.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500013

Risk factors for alloimmunization by patients with sickle cell disease
Provedor de dados: 56
Autores: Murao,M.; Viana,M.B.
Blood transfusion in patients with sickle cell disease (SCD) is limited by the development of alloantibodies to erythrocytes. In the present study, the frequency and risk factors for alloimmunization were determined. Transfusion records and medical charts of 828 SCD patients who had been transfused and followed at the Belo Horizonte Blood Center, Belo Horizonte, MG, Brazil, were retrospectively reviewed. Alloimmunization frequency was 9.9% (95% CI: 7.9 to 11.9%) and 125 alloantibodies were detected, 79% of which belonged to the Rhesus and Kell systems. Female patients developed alloimmunization more frequently (P = 0.03). The median age of the alloimmunized group was 23.3 years, compared to 14.6 years for the non-alloimmunized group (P &lt; 0.0001)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell disease; Red cell alloimmunization; Blood cell transfusion; Transfusion reactions.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500004

Evolution of circadian organization in vertebrates
Provedor de dados: 56
Circadian organization means the way in which the entire circadian system above the cellular level is put together physically and the principles and rules that determine the interactions among its component parts which produce overt rhythms of physiology and behavior. Understanding this organization and its evolution is of practical importance as well as of basic interest. The first major problem that we face is the difficulty of making sense of the apparently great diversity that we observe in circadian organization of diverse vertebrates. Some of this diversity falls neatly into place along phylogenetic lines leading to firm generalizations: i) in all vertebrates there is a "circadian axis" consisting of the retinas, the pineal gland and the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Circadian rhythms; Evolution; Vertebrates; Pineal; SCN; Retina; Melatonin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300003

Phagocytosis by macrophages mediated by receptors for denatured proteins - dependence on tyrosine protein kinases
Provedor de dados: 56
Autores: Hespanhol,M.R.; Mantovani,B.
Previous studies have demonstrated that some components of the leukocyte cell membrane, CR3 (Mac-1, CD11b/CD18) and p150/95, are able to bind to denatured proteins. Thus, it is of interest to know which effector functions of these cells can be triggered by these receptors when they interact with particles or surfaces covered with denatured proteins. In the present study we analyzed their possible role as mediators of phagocytosis of red cells covered with denatured bovine serum albumin (BSA) by mouse peritoneal macrophages. We observed that a) macrophages are able to recognize (bind to) these red cells, b) this interaction can be inhibited by denatured BSA in the fluid phase, c) there is no phagocytosis of these particles by normal macrophages, d)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phagocytosis; Denatured proteins; Macrophage; SS2 Integrins; Tyrosine protein kinases.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300015

c-Myc protein expression is not an independent prognostic predictor in cervical squamous cell carcinoma
Provedor de dados: 56
Autores: Brenna,S.M.F.; Zeferino,L.C.; Pinto,G.A.; Souza,R.A.; Andrade,L.A.L.; Vassalo,J.; Martinez,E.Z.; Syrjänen,K.J.
The c-myc protein is known to regulate the cell cycle, and its down-regulation can lead to cell death by apoptosis. The role of c-myc protein as an independent prognostic determinant in cervical cancer is controversial. In the present study, a cohort of 220 Brazilian women (mean age 53.4 years) with FIGO stage I, II and III (21, 28 and 51%, respectively) cervical squamous cell carcinomas was analyzed for c-myc protein expression using immunohistochemistry. The disease-free survival and relapse-rate were analyzed using univariate (Kaplan-Meier) survival analysis for 116 women who completed the standard FIGO treatment and were followed up for 5 years. Positive c-myc staining was detected in 40% of carcinomas, 29% being grade 1, 9% grade 2, and 2% grade 3....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-myc; Oncogene; Cervical cancer; Prognosis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400003

Swimming training attenuates oxidative damage and increases enzymatic but not non-enzymatic antioxidant defenses in the rat brain
Provedor de dados: 56
Autores: Nonato,L.F.; Rocha-Vieira,E.; Tossige-Gomes,R.; Soares,A.A.; Soares,B.A.; Freitas,D.A.; Oliveira,M.X.; Mendonça,V.A.; Lacerda,A.C.; Massensini,A.R.; Leite,H.R.
Although it is well known that physical training ameliorates brain oxidative function after injuries by enhancing the levels of neurotrophic factors and oxidative status, there is little evidence addressing the influence of exercise training itself on brain oxidative damage and data is conflicting. This study investigated the effect of well-established swimming training protocol on lipid peroxidation and components of antioxidant system in the rat brain. Male Wistar rats were randomized into trained (5 days/week, 8 weeks, 30 min; n=8) and non-trained (n=7) groups. Forty-eight hours after the last session of exercise, animals were euthanized and the brain was collected for oxidative stress analysis. Swimming training decreased thiobarbituric acid reactive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise therapy; Oxidative stress; Rat; Lipid peroxidation; Superoxide dismutase; Swimming training.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000605

Head-to-head comparison of dipyridamole, dobutamine and pacing stress echocardiography for the detection of myocardial ischemia in an animal model of coronary artery stenosis
Provedor de dados: 56
Autores: Schmidt,A.; de Almeida-Filho,O.C.; Ayres-Neto,E.M.; Carneiro,J.J.; Marin-Neto,J.A.; Maciel,B.C.
To compare the sensitivity of dipyridamole, dobutamine and pacing stress echocardiography for the detection of myocardial ischemia we produced a physiologically significant stenosis in the left circumflex artery of 14 open-chest dogs (range: 50 to 89% reduction in luminal diameter). In each study, dobutamine (5 to 40 µg kg-1 min-1 in 3-min stages) and pacing (20 bpm increments, each 2 min, up to 260 bpm) were performed randomly, and then followed by dipyridamole (up to 0.84 mg/kg over 10 min). The positivity of stress echocardiography tests was quantitatively determined by a significant (P&lt;0.05) reduction of or failure to increase absolute and percent systolic wall thickening in the stenotic artery supplied wall, as compared to the opposite wall (areas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress echocardiography; Pacing; Dobutamine; Dipyridamole; Animal model.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700010

Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients
Provedor de dados: 56
Autores: Nascimento,P.A.; da Silva,M.A.; Oliveira,E.M.; Suzuki,M.F.; Okazaki,K.
Genetic damage caused by ionizing radiation and repair capacity of blood lymphocytes from 3 breast cancer patients and 3 healthy donors were investigated using the comet assay. The comets were analyzed by two parameters: comet tail length and visual classification. Blood samples from the donors were irradiated in vitro with a 60Co source at a dose rate of 0.722 Gy/min, with a dose range of 0.2 to 4.0 Gy and analyzed immediately after the procedure and 3 and 24 h later. The basal level of damage and the radioinduced damage were higher in lymphocytes from breast cancer patients than in lymphocytes from healthy donors. The radioinduced damage showed that the two groups had a similar response when analyzed immediately after the irradiations. Therefore, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; DNA damage; DNA repair; Ionizing radiation; Breast cancer; Blood lymphocytes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200003

Hospital strain colonization by Staphylococcus epidermidis
Provedor de dados: 56
Autores: Blum-Menezes,D.; Bratfich,O.J.; Padoveze,M.C.; Moretti,M.L.
The skin and mucous membranes of healthy subjects are colonized by strains of Staphylococcus epidermidis showing a high diversity of genomic DNA polymorphisms. Prolonged hospitalization and the use of invasive procedures promote changes in the microbiota with subsequent colonization by hospital strains. We report here a patient with prolonged hospitalization due to chronic pancreatitis who was treated with multiple antibiotics, invasive procedures and abdominal surgery. We studied the dynamics of skin colonization by S. epidermidis leading to the development of catheter-related infections and compared the genotypic profile of clinical and microbiota strains by pulsed field gel electrophoresis. During hospitalization, the normal S. epidermidis skin...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylococcus epidermidis; Microbiota; Biofilm; Central venous catheter.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300011

GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis
Provedor de dados: 56
Autores: Seraphim,P.M.; Nunes,M.T.; Machado,U.F.
GLUT4 protein expression in white adipose tissue (WAT) and skeletal muscle (SM) was investigated in 2-month-old, 12-month-old spontaneously obese or 12-month-old calorie-restricted lean Wistar rats, by considering different parameters of analysis, such as tissue and body weight, and total protein yield of the tissue. In WAT, a ~70% decrease was observed in plasma membrane and microsomal GLUT4 protein, expressed as µg protein or g tissue, in both 12-month-old obese and 12-month-old lean rats compared to 2-month-old rats. However, when plasma membrane and microsomal GLUT4 tissue contents were expressed as g body weight, they were the same. In SM, GLUT4 protein content, expressed as µg protein, was similar in 2-month-old and 12-month-old obese rats, whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin resistance; Obesity; Weight loss; GLUT4 protein.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000018

Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway
Provedor de dados: 56
Autores: Wang,J.; Lu,M.L.; Dai,H.L.; Zhang,S.P.; Wang,H.X.; Wei,N.
This study investigated the in vitro and in vivo antiproliferative activity of esculetin against hepatocellular carcinoma, and clarified its potential molecular mechanisms. Cell viability was determined by the MTT (tetrazolium) colorimetric assay. In vivo antitumor activity of esculetin was evaluated in a hepatocellular carcinoma mouse model. Seventy-five C57BL/6J mice were implanted with Hepa1-6 cells and randomized into five groups (n=15 each) given daily intraperitoneal injections of vehicle (physiological saline), esculetin (200, 400, or 700 mg·kg-1·day-1), or 5-Fu (200 mg·kg-1·day-1) for 15 days. Esculetin significantly decreased tumor growth in mice bearing Hepa1-6 cells. Tumor weight was decreased by 20.33, 40.37, and 55.42% with increasing doses of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esculetin; Apoptosis; Hepatocellular carcinoma; Mitochondrial-dependent pathway; Antitumor activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300245

Effects of exposure to fine particulate matter in elderly hospitalizations due to respiratory diseases in the South of the Brazilian Amazon
Provedor de dados: 56
Autores: Machin,A.B.; Nascimento,L.F.; Mantovani,K.; Machin,E.B.
Exposure to air pollution is an important cause of hospital admissions due to respiratory diseases. Nevertheless, few studies use pollutant concentration data estimated by mathematical models. A time-series ecological study was developed, using data from hospitalizations due to respiratory diseases in people over 60 years of age, residents of Cuiabá, Brazil, during 2012, obtained from the Brazilian Ministry of Health. The independent variables were the concentrations of fine particulate matter (PM2.5) and carbon monoxide (CO) estimated by mathematical modeling, minimum temperature, and relative humidity (obtained from the Brazilian Meteorological Agency), and the number of forest fires. The generalized linear regression model of Poisson was used, with lags...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollutants; Particulate matter; Respiratory diseases; Elderly health; Mathematical modeling.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200604

Characterization of alpha thalassemic genotypes by multiplex ligation-dependent probe amplification in the Brazilian population
Provedor de dados: 56
Autores: Suemasu,C.N.; Kimura,E.M.; Oliveira,D.M.; Bezerra,M.A.C.; Araújo,A.S.; Costa,F.F.; Sonati,M.F.
Alpha-thalassemia is the most common inherited disorder of hemoglobin synthesis. Genomic deletions involving the alpha-globin gene cluster on chromosome 16p13.3 are the most frequent molecular causes of the disease. Although common deletions can be detected by a single multiplex gap-PCR, the rare and novel deletions depend on more laborious techniques for their identification. The multiplex ligation-dependent probe amplification (MLPA) technique has recently been used for this purpose and was successfully used in the present study to detect the molecular alterations responsible for the alpha-thalassemic phenotypes in 8 unrelated individuals (3 males and 5 females; age, 4 months to 30 years) in whom the molecular basis of the disease could not be determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-thalassemia; Hb H disease; Multiplex ligation-dependent probe amplification; Genetic polymorphisms; Brazilian population.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100003

Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
Provedor de dados: 56
Autores: Hermel,E.E.S.; Severino,G.S.; Cecconello,A.L.; Pereira,F.M.; Sanvitto,G.L.; Lucion,A.B.
Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotin-peroxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Stress; Neonatal stimulation; Dopamine; Immunohistochemistry; Hypothalamus; Tyrosine hydroxylase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900013

The modulation of simple reaction time by the spatial probability of a visual stimulus
Provedor de dados: 56
Autores: Carreiro,L.R.R.; Haddad Jr.,H.; Baldo,M.V.C.
Simple reaction time (SRT) in response to visual stimuli can be influenced by many stimulus features. The speed and accuracy with which observers respond to a visual stimulus may be improved by prior knowledge about the stimulus location, which can be obtained by manipulating the spatial probability of the stimulus. However, when higher spatial probability is achieved by holding constant the stimulus location throughout successive trials, the resulting improvement in performance can also be due to local sensory facilitation caused by the recurrent spatial location of a visual target (position priming). The main objective of the present investigation was to quantitatively evaluate the modulation of SRT by the spatial probability structure of a visual...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Simple reaction time; Spatial probability; Vision; Attention.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700011

Prevalence of the STK15 F31I polymorphism and its relationship with mammographic density
Provedor de dados: 56
Autores: Giacomazzi,J.; Aguiar,E.; Palmero,E.I.; Schmidt,A.V.; Skonieski,G.; Duarte Filho,D.; Bock,H.; Saraiva-Pereira,M.L.; Schuler-Faccini,L.; Camey,S.A.; Caleffi,M.; Giugliani,R.; Ashton-Prolla,P.
Several studies have identified the single nucleotide polymorphism STK15 F31I as a low-penetrance risk allele for breast cancer, but its prevalence and risk association in the Brazilian population have not been determined. The goal of this study was to identify the frequency of this polymorphism in the Brazilian setting. Considering the high degree of admixture of our population, it is of fundamental importance to validate the results already reported in the literature and also to verify the relationship between this variant and breast cancer risk. A total of 750 women without breast cancer were genotyped using the TaqMan PCR assay for STK15 F31I polymorphism. Clinical information was obtained from review of the medical records and mammographic density...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: STK15 F31I polymorphism; Breast cancer risk; Mammographic density.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400004

Regulation of the expression of NADP-malic enzyme by UV-B, red and far-red light in maize seedlings
Provedor de dados: 56
Autores: Casati,P.; Drincovich,M.F.; Edwards,G.E.; Andreo,C.S.
The induction of nicotinamide adenine dinucleotide phosphate-malic enzyme (NADP-ME) in etiolated maize (Zea mays) seedlings by UV-B and UV-A radiation, and different levels of photosynthetically active radiation (PAR, 400-700 nm) was investigated by measuring changes in activity, protein quantity and RNA levels as a function of intensity and duration of exposure to the different radiations. Under low levels of PAR, exposure to UV-B radiation but not UV-A radiation for 6 to 24 h caused a marked increase in the enzyme levels similar to that observed under high PAR in the absence of UV-B. UV-B treatment of green leaves following a 12-h dark period also caused an increase in NADP-ME expression. Exposure to UV-B radiation for only 5 min resulted in a rapid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C4 photosynthesis; NADP-malic enzyme; UV-B radiation; Photosynthesis; Red light; Far-red light.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000002

Clinical, epidemiological, and microbiological characteristics of bacteremia caused by high-level gentamicin-resistant Enterococcus faecalis
Provedor de dados: 56
Autores: Vigani,A.G.; Macedo de Oliveira,A.; Bratfich,O.J.; Stucchi,R.S.B.; Moretti,M.L.
Enterococcus spp bacteremia is associated with high mortality and the appearance of high-level gentamicin resistance (HLGR) created additional challenges for the treatment of these infections. We evaluated the epidemiological and clinical characteristics of patients with bacteremias caused by HLGR and non_HLGR Enterococcus faecalis isolates at a teaching hospital in the State of São Paulo, Brazil. Patients with bacteremia due to E. faecalis diagnosed between January 1999 and December 2003 were included in the study. We collected clinical, epidemiological, and microbiological data from medical records. Banked isolates were typed using pulsed-field gel electrophoresis. We identified 145 cases of E. faecalis bacteremia: 66 (45.5%) were caused by HLGR isolates...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterococcus faecalis; Bacteremia; High-level gentamicin resistance; Infection control; Enterococcus faecalis genotyping.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000010

Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts
Provedor de dados: 56
Autores: Zhang,L.; Ding,Y.; Rao,G.Z.; Miao,D.
The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P&lt;0.05), both in a concentration-dependent manner. Glucose stimulation had the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human periodontal ligament fibroblasts; Interleukin-10; Glucose; Osteoprotegerin; Receptor activator of nuclear factor-κB ligand.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605

Arsenic trioxide reduces the invasive and metastatic properties of nasopharyngeal carcinoma cells in vitro
Provedor de dados: 56
Autores: Du,C.W.; Wen,B.G.; Li,D.R.; Peng,X.; Hong,C.Q.; Chen,J.Y.; Lin,Z.Z.; Hong,X.; Lin,Y.C.; Xie,L.X.; Wu,M.Y.; Zhang,H.
Nasopharyngeal carcinoma (NPC) is notorious for the metastases, which are in close association with Epstein-Barr virus-encoded latent membrane protein 1 (LMP1). Arsenic trioxide (As2O3) has been shown to induce apoptosis and differentiation in NPC xenografts. Then, can it repress the cancer cells' metastasis potential? To elucidate this issue, the present study was performed. LMP1-negative cell line HNE1 and LMP1-positive cell line HNE1-LMP1 were used as in vitro model. Cells (1 x 10(5)/mL) were cultured with or without 3 µM As2O3 for 48 h. Then the survival cells were collected to investigate their potential of colony formation, attachment, invasion, and migration. Both confocal immunofluorescence staining and Western blot were used to detect the changes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arsenic trioxide (As2O3); Nasopharyngeal carcinoma; Metastases; Latent membrane protein 1; Metalloproteinase 9.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500015

Endothelial cells, tissue factor and infectious diseases
Provedor de dados: 56
Autores: Lopes-Bezerra,L.M.; Filler,S.G.
Tissue factor is a transmembrane procoagulant glycoprotein and a member of the cytokine receptor superfamily. It activates the extrinsic coagulation pathway, and induces the formation of a fibrin clot. Tissue factor is important for both normal homeostasis and the development of many thrombotic diseases. A wide variety of cells are able to synthesize and express tissue factor, including monocytes, granulocytes, platelets and endothelial cells. Tissue factor expression can be induced by cell surface components of pathogenic microorganisms, proinflammatory cytokines and membrane microparticles released from activated host cells. Tissue factor plays an important role in initiating thrombosis associated with inflammation during infection, sepsis, and organ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue factor; Endothelial cell; Fungus; Infectious diseases; Procoagulant activity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800004

Combined aliskiren and L-arginine treatment has antihypertensive effects and prevents vascular endothelial dysfunction in a model of renovascular hypertension
Provedor de dados: 56
Autores: Santuzzi,C.H.; Tiradentes,R.V.; Mengal,V.; Claudio,E.R.G.; Mauad,H.; Gouvea,S.A.; Abreu,G.R.
Angiotensin II is a key player in the pathogenesis of renovascular hypertension, a condition associated with endothelial dysfunction. We investigated aliskiren (ALSK) and L-arginine treatment both alone and in combination on blood pressure (BP), and vascular reactivity in aortic rings. Hypertension was induced in 40 male Wistar rats by clipping the left renal artery. Animals were divided into Sham, 2-kidney, 1-clip (2K1C) hypertension, 2K1C+ALSK (ALSK), 2K1C+L-arginine (L-arg), and 2K1C+ALSK+L-arginine (ALSK+L-arg) treatment groups. For 4 weeks, BP was monitored and endothelium-dependent and independent vasoconstriction and relaxation were assessed in aortic rings. ALSK+L-arg reduced BP and the contractile response to phenylephrine and improved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2K1C hypertension; Endothelial dysfunction; Aliskiren; L-arginine; RAAS and oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100065

Antiviral activity of shikonin ester derivative PMM-034 against enterovirus 71 in vitro
Provedor de dados: 56
Autores: Zhang,Y.; Han,H.; Sun,L.; Qiu,H.; Lin,H.; Yu,L.; Zhu,W.; Qi,J.; Yang,R.; Pang,Y.; Wang,X.; Lu,G.; Yang,Y.
Human enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD), particularly in infants and children below 4 years of age. Shikonin is a bioactive compound with anti-inflammatory, antiviral, and antibacterial activities derived from the roots of the Chinese medicinal herb Lithospermum erythrorhizon. This study aimed to examine the antiviral activity of PMM-034, a shikonin ester derivative, against EV71 in rhabdomyosarcoma (RD) cells. Cytotoxicity of PMM-034 on RD cells was determined using WST-1 assay. Dose- and time-dependent effects of PMM-034 on EV71 replication in RD cells were determined using plaque reduction assay. mRNA expression levels of EV71/VP1 and pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EV71; VP1; Shikonin ester derivatives PMM-034; Rhabdomyosarcoma cells; NF-κB.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000602

Short-term creatine supplementation changes protein metabolism signaling in hindlimb suspension
Provedor de dados: 56
Autores: Marzuca-Nassr,G.N.; Fortes,M.A.S.; Guimarães-Ferreira,L.; Murata,G.M.; Vitzel,K.F.; Vasconcelos,D.A.A.; Bassit,R.A.; Curi,R.
The effect of a short-term creatine supplementation on hindlimb suspension (HS)-induced muscle atrophy was investigated. Creatine monohydrate (5 g/kg b.w. per day) or placebo, divided in 2 daily doses, was given by oral gavage for 5 days. Rats were maintained in HS with dietary supplementation concomitantly for 5 days. Body weight, soleus and EDL muscle masses, and cross-sectional areas (CSA) of the muscle fibers were measured. Signaling pathways associated with skeletal muscle mass regulation (FST, MSTN, FAK, IGF-1, MGF, Akt, mTOR, atrogin-1, and MuRF1 expressions, and Akt, S6, GSK3B, and 4EBP1 proteins) were evaluated in the muscles. Soleus muscle exhibited more atrophy than the EDL muscle due to HS. Creatine supplementation attenuated the decrease of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Creatine supplementation; Hindlimb suspension; Muscle disuse atrophy; Protein synthesis; Protein degradation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000606

Weight of preterm newborns during the first twelve weeks of life
Provedor de dados: 56
Autores: Anchieta,L.M.; Xavier,C.C.; Colosimo,E.A.; Souza,M.F.
A longitudinal and prospective study was carried out at two state-operated maternity hospitals in Belo Horizonte during 1996 in order to assess the weight of preterm appropriate-for-gestational-age newborns during the first twelve weeks of life. Two hundred and sixty appropriate-for-gestational-age preterm infants with birth weight &lt;2500 g were evaluated weekly. The infants were divided into groups based on birth weight at 250-g intervals. Using weight means, somatic growth curves were constructed and adjusted to Count's model. Absolute (g/day) and relative (g kg-1 day-1) velocity curves were obtained from a derivative of this model. The growth curve was characterized by weight loss during the 1st week (4-6 days) ranging from 5.9 to 13.3% (the greater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premature infants; Growth curves; Growth velocity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600012

Penile microvascular endothelial function in hypertensive patients: effects of acute type 5 phosphodiesterase inhibition
Provedor de dados: 56
Autores: Verri,V.; Brandão,A.A.; Tibirica,E.
The primary aim of this study was to evaluate penile endothelial microvascular function in patients with primary arterial hypertension and age-matched normotensive subjects using laser speckle contrast imaging (LSCI). Additionally, we analyzed the acute penile microvascular effects induced by oral phosphodiesterase type 5 inhibitor (sildenafil; SIL) administration. Endothelium-dependent microvascular reactivity was evaluated in the penises and forearms of hypertensive patients (aged 58.8±6.6 years, n=34) and age-matched healthy volunteers (n=33) at rest and 60 min following oral SIL (100 mg) administration. LSCI was coupled with cutaneous acetylcholine (ACh) iontophoresis using increasing anodal currents. Basal penile cutaneous vascular conductance (CVC)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microvascular dysfunction; Hypertension; Laser speckle contrast imaging; Penile microcirculation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300607

Exploratory calcineurin inhibitor-free regimens in living-related kidney transplant recipients
Provedor de dados: 56
Autores: Garcia,R.; Machado,P.G.; Felipe,C.R.; Park,S.I.; Spinelli,G.A.; Franco,M.F.; Tedesco-Silva Jr.,H.; Medina-Pestana,J.O.
Chronic allograft nephropathy is among the major causes of graft loss even in low-risk kidney transplant recipients and correlates with acute nephrotoxic events during the first year post-transplant. Therefore, calcineurin inhibitor-free regimens may improve patient and graft survival among recipients of living-related kidney transplants. To confirm this hypothesis, we evaluated the efficacy and safety of two calcineurin inhibitor-free regimens in 92 low-risk recipients of one-haplotype living-related kidney transplants. Immunosuppression consisted of tacrolimus, azathioprine and prednisone (group I, GI, N = 38), 2 doses of daclizumab, mycophenolate mofetil (MMF), and prednisone (GII, N = 33) and 2 doses of daclizumab, MMF, sirolimus and prednisone (GIII,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tacrolimus; Mycophenolate mofetil; Sirolimus; Kidney transplantation; Acute rejection; Clinical trial.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400003

Neuroethologic differences in sleep deprivation induced by the single- and multiple-platform methods
Provedor de dados: 56
Autores: Medeiros,R.; Lenneberg-Hoshino,C.; Hoshino,K.; Tufik,S.
It has been proposed that the multiple-platform method (MP) for desynchronized sleep (DS) deprivation eliminates the stress induced by social isolation and by the restriction of locomotion in the single-platform (SP) method. MP, however, induces a higher increase in plasma corticosterone and ACTH levels than SP. Since deprivation is of heuristic value to identify the functional role of this state of sleep, the objective of the present study was to determine the behavioral differences exhibited by rats during sleep deprivation induced by these two methods. All behavioral patterns exhibited by a group of 7 albino male Wistar rats submitted to 4 days of sleep deprivation by the MP method (15 platforms, spaced 150 mm apart) and by 7 other rats submitted to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sleep deprivation methods; Stress; Social interactions; Group crowding effects.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500012

Influences of alpha-tocopherol on cholesterol metabolism and fatty streak development in apolipoprotein E-deficient mice fed an atherogenic diet
Provedor de dados: 56
Autores: Peluzio,M.C.G.; Homem,A.P.P.; Cesar,G.C.; Azevedo,G.S.; Amorim,R.; Cara,D.C.; Saliba,H.; Vieira,E.C.; Arantes,R.E.; Alvarez-Leite,J.
Although the role of oxidized lipoproteins is well known in atherogenesis, the role of vitamin E supplementation is still controversial. There is also little information about cholesterol metabolism (hepatic concentration and fecal excretion) in the new models of atherosclerosis. In the present study, we evaluated the effect of moderate vitamin E supplementation on cholesterol metabolism and atherogenesis in apolipoprotein E (apo E)-deficient mice. Apo E-deficient mice were fed an atherogenic diet containing 40 or 400 mg/kg of alpha-tocopherol acetate for 6 weeks. Total cholesterol in serum and liver and 3-OH-alpha-sterols in feces, and fecal excretion of bile acids were determined and histological analyses of aortic lesion were performed. A vitamin E-rich...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Atherosclerosis; Apo E; Mice; Alpha-tocopherol; Cholesterol.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200005

In vitro larvicidal activity of geraniol and citronellal against Contracaecum sp (Nematoda: Anisakidae)
Provedor de dados: 56
Autores: Barros,L.A.; Yamanaka,A.R.; Silva,L.E.; Vanzeler,M.L.A.; Braum,D.T.; Bonaldo,J.
Human infection with fish parasites can result from the ingestion of incompletely cooked or raw fish, giving origin to parasitic diseases such as anisakiasis, caused by parasites of the Anisakidae family. The present study assessed the in vitro larvicidal effect of two monoterpene compounds, geraniol and citronellal, against Contracaecum sp (Nematoda: Anisakidae). Four hundred live larvae of Contracaecum sp obtained from "traíra" fish (Hoplias malabaricus, Bloch, 1974) were analyzed on 40 Petri dishes (10 larvae each) with the compounds to be tested. The final concentrations tested for each compound were 250, 125, 62.5, and 31.2 µg/mL and the evaluation was carried out at five different times (2, 4, 8, 24, and 48 h). The larvicidal action of geraniol and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Geraniol; Citronellal; Larvicide; Contracaecum sp.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000007

A forced running wheel system with a microcontroller that provides high-intensity exercise training in an animal ischemic stroke model
Provedor de dados: 56
Autores: Chen,C.C.; Chang,M.W.; Chang,C.P.; Chan,S.C.; Chang,W.Y.; Yang,C.L.; Lin,M.T.
We developed a forced non-electric-shock running wheel (FNESRW) system that provides rats with high-intensity exercise training using automatic exercise training patterns that are controlled by a microcontroller. The proposed system successfully makes a breakthrough in the traditional motorized running wheel to allow rats to perform high-intensity training and to enable comparisons with the treadmill at the same exercise intensity without any electric shock. A polyvinyl chloride runway with a rough rubber surface was coated on the periphery of the wheel so as to permit automatic acceleration training, and which allowed the rats to run consistently at high speeds (30 m/min for 1 h). An animal ischemic stroke model was used to validate the proposed system....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forced non-electric-shock running wheel; Middle cerebral artery occlusion; Inclined plane test; Modified neurological severity score; Triphenyltetrazolium chloride.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000858

Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines
Provedor de dados: 56
Autores: Castro,M.A.A.; Schwartsmann,G.; Moreira,J.C.F.
In the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5% CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine ß staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 10³ cells/cm² among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-small cell lung carcinoma; Cell density; Intercellular contact; Cell phenotype; UV radiation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800006

High quality human immunoglobulin G purified from Cohn fractions by liquid chromatography
Provedor de dados: 56
Autores: Tanaka,K.; Sawatani,E.; Dias,G.A.; Shigueoka,E.M.; Campos,T.C.X.B.; Nakao,H.C.; Arashiro,F.
In order to obtain intravenous immunoglobulin G (iv IgG) of high quality from F-I+II+III or F-II+III pastes prepared by the Cohn method, we developed a chromatography process using ion exchange gels, Q-Sepharose FF and CM-Sepharose FF, and Sephacryl S-300 gel filtration. Viral inactivation was performed by incubating the preparation with pepsin at pH 4.0 at 35oC for 18 h. The characteristics of 28 batches produced by us were: yield 4.3 ± 0.2 g/l plasma, i.e., a recovery of 39.1 ± 1.8%; IgG subclasses distribution: IgG1 = 58.4%, IgG2 = 34.8%, IgG3 = 4.5% and IgG4 = 2.3%; IgG size distribution was 98.4% monomers, 1.2% dimers and 0.4% polymers and protein aggregates; anticomplement activity was less than 0.5 CH50/mg IgG, and prekallikrein activator activity...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Immunoglobulin G purification; Hemoderivative purification; Chromatographic procedures; High quality immunoglobulin G; Downstream process.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100004

Trends in drug use among students in Brazil: analysis of four surveys in 1987, 1989, 1993 and 1997
Provedor de dados: 56
Autores: Galduróz,J.C.F.; Noto,A.R.; Nappo,S.A.; Carlini,E.A.
The consumption of psychotropic drugs among Brazilian secondary school students was examined by comparing data from four surveys using a questionnaire adapted from the WHO's Program on Research and Reporting on the Epidemiology of Drug Dependence. Students filled out the form in their classrooms without the presence of teachers. The target population consisted of 10-18-year-old students (on average, 15,000 students responded to each survey) in Brazil's ten largest state capitals: Belém, Belo Horizonte, Brasília, Curitiba, Fortaleza, Porto Alegre, Recife, Rio de Janeiro, Salvador, and São Paulo. Among the legal drugs, lifetime use (use at least once during life) of tobacco was increased in seven cities (the exceptions were Brasília, Porto Alegre and Rio de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Students; Trends; Inhalants; Cocaine; Marijuana; Surveys; Brazilian students.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400009

Detection of sputum cofilin-1 as indicator of malignancy
Provedor de dados: 56
Autores: Rangel,M.P.; Antonangelo,L.; Acencio,M.M.P.; Faria,C.S.; de Sá,V.K.; Leão,P.S.; Farhat,C.; Fabro,A.T.; Longatto Filho,A.; Reis,R.M.; Takagaki,T.; Capelozzi,V.L.
Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P&lt;0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung cancer; Cofilin-1; Sputum; Liquid-biopsy; Metastases; Biomarker.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800603

Antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects
Provedor de dados: 56
Autores: Mariano,F.S.; Campanelli,A.P.; Nociti Jr.,F.H.; Mattos-Graner,R.O.; Gonçalves,R.B.
Neutrophils play an important role in periodontitis by producing nitric oxide (NO) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. It is unknown whether variation in the production of antimicrobial peptides such as LL-37, human neutrophil peptides (HNP) 1-3, and NO by neutrophils influences the pathogenesis of periodontal diseases. We compared the production of these peptides and NO by lipopolysaccharide (LPS)-stimulated neutrophils isolated from healthy subjects and from patients with periodontitis. Peripheral blood neutrophils were cultured with or without Aggregatibacter actinomycetemcomitans-LPS (Aa-LPS), Porphyromonas gingivalis-LPS (Pg-LPS) and Escherichia coli-LPS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial peptides; Nitric oxide; Neutrophils; Periodontitis; Innate immunity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100004

Evidence for the endophytic colonization of Phaseolus vulgaris(common bean) roots by the diazotroph Herbaspirillum seropedicae
Provedor de dados: 56
Autores: Schmidt,M.A.; Souza,E.M.; Baura,V.; Wassem,R.; Yates,M.G.; Pedrosa,F.O.; Monteiro,R.A.
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8) CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g. The identity of the recovered bacteria was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbaspirillum seropedicae; Phaseolus vulgaris; Confocal microscopy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300001

Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system
Provedor de dados: 56
Autores: Chuang,K.A.; Lieu,C.H.; Tsai,W.J.; Wu,M.H.; Chen,Y.C.; Liao,J.F.; Wang,C.C.; Kuo,Y.C.
Refractory and relapsed leukemia is a major problem during cancer therapy, which is due to the aberrant activation of Wnt/β-catenin signaling pathway. Activation of this pathway is promoted by wingless (Wnt) proteins and induces co-activator β-catenin binding to lymphoid enhancer factor (LEF)/T-cell factor protein (TCF). To provide a convenient system for the screening of anti-Wnt/β-catenin agents, we designed a bi-functional pGL4-TOP reporter plasmid that contained 3X β-catenin/LEF/TCF binding sites and a selectable marker. After transfection and hygromycin B selection, HEK 293-TOP and Jurkat-TOP stable clones were established. The luciferase activity in the stable clone was enhanced by the recombinant Wnt-3A (rWnt-3A; 100-400 ng/mL) and GSK3β inhibitor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Plasmids; Reporter assay; Screening; Wnt; Β-catenin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000003

Characterization and analysis of the outcome of adults with acute myeloid leukemia treated in a Brazilian University hospital over three decades
Provedor de dados: 56
Autores: Souto Filho,J.T.; Portugal,R.D.; Loureiro,M.; Pulcheri,W.; Nucci,M.
We evaluated the outcome of 227 patients with acute myeloid leukemia during three decades (period 1 - 1980’s, N = 89; period 2 - 1990’s, N = 73; period 3 - 2000’s, N = 65) at a single institution. Major differences between the three groups included a higher median age, rates of multilineage dysplasia and co-morbidities, and a lower rate of clinical manifestations of advanced leukemia in recent years. The proportion of patients who received induction remission chemotherapy was 66, 75, and 85% for periods 1, 2, and 3, respectively (P = 0.04). The median survival was 40, 77, and 112 days, and the 5-year overall survival was 7, 13, and 22%, respectively (P = 0.01). The median disease-free survival was 266, 278, and 386 days (P = 0.049). Survival expectation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; Survival; Prognosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700008

Relevance of apolipoprotein E4 for the lipid profile of Brazilian patients with coronary artery disease
Provedor de dados: 56
Autores: Souza,D.R.S.; Nakachima,L.; Biagioni,R.B.; Nakazone,M.A.; Pinhel,M.A.S.; Trindade,D.M.; Mafra,V.T.; Tácito,L.H.B.; Martin,J.F.V.; Pinheiro Júnior,S.; Brandão,A.C.
Apolipoprotein E (apoE - e2, e3, e4 alleles) plays a role in the regulation of lipid metabolism, with the e4 considered to be a risk factor for coronary artery disease (CAD). We aimed to evaluate the apoE polymorphisms in Brazilians with CAD and their influence on the lipid profile and other risk factors (hypertension, diabetes mellitus, smoking). Two hundred individuals were examined: 100 patients with atherosclerosis confirmed by coronary angiography and 100 controls. Blood samples were drawn to determine apoE polymorphisms and lipid profile. As expected, the e3 allele was prevalent in the CAD (0.87) and non-CAD groups (0.81; P = 0.099), followed by the e4 allele (0.09 and 0.14, respectively; P = 0.158). The e3/3 (76 and 78%) and e3/4 (16 and 23%) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E polymorphism; Coronary artery disease; Lipid profile.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200005

Mechanical cardiac remodeling and new-onset atrial fibrillation in long-term follow-up of subjects with chronic Chagas' disease
Provedor de dados: 56
Autores: Benchimol-Barbosa,P.R.; Barbosa-Filho,J.
Atrial fibrillation (AF) affects subjects with Chagas' disease and is an indicator of poor prognosis. We investigated clinical, echocardiographic and electrocardiographic variables of Chagas' disease in a long-term longitudinal study as predictors of a new-onset AF episode lasting &gt;24 h, nonfatal embolic stroke and cardiac death. Fifty adult outpatients (34 to 74 years old, 62% females) staged according to the Los Andes classification were enrolled. During a follow-up of (mean ± SD) 84.2 ± 39.0 months, 9 subjects developed AF (incidence: 3.3 ± 1.0%/year), 5 had nonfatal stroke (incidence: 1.3 ± 1.0%/year), and nine died (mortality rate: 2.3 ± 0.8%/year). The progression rate of left ventricular mass and left ventricular ejection fraction was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas' disease; Cardiomyopathy; Atrial fibrillation; Cardiac death; Embolic stroke; Prognosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300006

Cellular signaling with nitric oxide and cyclic GMP
Provedor de dados: 56
Autores: Murad,F.
During the past two decades, nitric oxide signaling has been one of the most rapidly growing areas in biology. This simple free radical gas can regulate an ever growing list of biological processes. In most instances nitric oxide mediates its biological effects by activating guanylyl cyclase and increasing cyclic GMP synthesis. However, the identification of effects of nitric oxide that are independent of cyclic GMP is also growing at a rapid rate. The effects of nitric oxide can mediate important physiological regulatory events in cell regulation, cell-cell communication and signaling. Nitric oxide can function as an intracellular messenger, neurotransmitter and hormone. However, as with any messenger molecule, there can be too much or too little of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Nitric oxide synthase; Guanylyl cyclase; Cell signaling.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100001

The 5alpha-reductase type 1, but not type 2, gene is expressed in anagen hairs plucked from the vertex area of the scalp of hirsute women and normal individuals
Provedor de dados: 56
Autores: Oliveira,I.O.; Lhullier,C.; Brum,I.S.; Spritzer,P.M.
The aim of the present study was to determine the expression of the genes for type 1 (SDR5A1) and type 2 (SDR5A2) 5alpha-reductase isoenzymes in scalp hairs plucked from 33 hirsute patients (20 with polycystic ovary syndrome and 13 with idiopathic hirsutism) and compare it with that of 10 men and 15 normal women. SDR5A1 and SDR5A2 expression was estimated by RT-PCR using the gene of the ubiquitously expressed protein ß2-microglobulin as an internal control. The results are expressed as arbitrary units in relation to ß2-microglobulin absorbance (mean ± SEM). SDR5A2 expression was not detected in any hair samples analyzed in this study. No differences were found in SDR5A1 mRNA levels between men and normal women (0.78 ± 0.05 vs 0.74 ± 0.06, respectively)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hair follicle; Hirsutism; 5alpha-Reductase; Polycystic ovary syndrome.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000024

Ischemia and fibrosis: the risk mechanisms of hypertensive heart disease
Provedor de dados: 56
Autores: Frohlich,E.D.
Mechanisms underlying risk associated with hypertensive heart disease (HHD) and left ventricular hypertrophy (LVH) are discussed in this report and provide a rationale for understanding this very common and important cause of death from hypertension and its complications. Emphasized are impaired coronary hemodynamics, endothelial dysfunction, and ventricular fibrosis from increased collagen deposition intramurally and perivascularly. Each is exacerbated by aging and, perhaps, also by increased dietary salt intake. These functional and structural changes promote further endothelial dysfunction, altered coronary hemodynamics, and diastolic as well as systolic ventricular contractile function in HHD. The clinical endpoints of HHD include angina pectoris (with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Left ventricular hypertrophy; LVH; Risk factor; Myocardial ischemia; Ventricular fibrosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600010

Effect of intraperitoneally administered hydrolyzed whey protein on blood pressure and renal sodium handling in awake spontaneously hypertensive rats
Provedor de dados: 56
Autores: Costa,E.L.; Almeida,A.R.; Netto,F.M.; Gontijo,J.A.R.
The present study evaluated the acute effect of the intraperitoneal (ip) administration of a whey protein hydrolysate (WPH) on systolic arterial blood pressure (SBP) and renal sodium handling by conscious spontaneously hypertensive rats (SHR). The ip administration of WPH in a volume of 1 ml dose-dependently lowered the SBP in SHR 2 h after administration at doses of 0.5 g/kg (0.15 M NaCl: 188.5 ± 9.3 mmHg vs WPH: 176.6 ± 4.9 mmHg, N = 8, P = 0.001) and 1.0 g/kg (0.15 M NaCl: 188.5 ± 9.3 mmHg vs WPH: 163.8 ± 5.9 mmHg, N = 8, P = 0.0018). Creatinine clearance decreased significantly (P = 0.0084) in the WPH-treated group (326 ± 67 µL min-1 100 g body weight-1) compared to 0.15 M NaCl-treated (890 ± 26 µL min-1 100 g body weight-1) and captopril-treated (903...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arterial blood pressure; Hydrolysis; Renal function; Urinary sodium excretion; Whey protein isolate.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200010

Thalassemia intermedia as a result of heterozygosis for ß0-thalassemia and aaaanti-3.7/aa genotype in a Brazilian patient
Provedor de dados: 56
Autores: Kimura,E.M.; Grignoli,C.R.E.; Pinheiro,V.R.P.; Costa,F.F.; Sonati,M.F.
We report a case in which the interaction of heterozygosis for both the ß0-IVS-II-1 (G-&gt;A) mutation and the &lt;FONT FACE=Symbol&gt;aaa&lt;/FONT&gt;anti-3.7 allele was the probable cause for the clinical occurrence of thalassemia intermedia. The propositus, a 6-year-old Caucasian Brazilian boy of Portuguese descent, showed a moderately severe chronic anemia in spite of having the ß-thalassemia trait. Investigation of the alpha-globin gene status revealed heterozygosis for alpha-gene triplication (&lt;FONT FACE=Symbol&gt;aaa&lt;/FONT&gt;/&lt;FONT FACE=Symbol&gt;aa&lt;/FONT&gt;). The patient's father, also presenting mild microcytic and hypochromic anemia, had the same alpha and ß genotypes as his son, while the mother, not related to the father and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SS-Thalassemia; Thalassemia intermedia; Triplicated alpha-globin genes; Hemoglobinopathies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600003

Correlation between heart rate variability and pulmonary function adjusted by confounding factors in healthy adults
Provedor de dados: 56
Autores: Bianchim,M.S.; Sperandio,E.F.; Martinhão,G.S.; Matheus,A.C.; Lauria,V.T.; da Silva,R.P.; Spadari,R.C.; Gagliardi,A.R.T.; Arantes,R.L.; Romiti,M.; Dourado,V.Z.
The autonomic nervous system maintains homeostasis, which is the state of balance in the body. That balance can be determined simply and noninvasively by evaluating heart rate variability (HRV). However, independently of autonomic control of the heart, HRV can be influenced by other factors, such as respiratory parameters. Little is known about the relationship between HRV and spirometric indices. In this study, our objective was to determine whether HRV correlates with spirometric indices in adults without cardiopulmonary disease, considering the main confounders (e.g., smoking and physical inactivity). In a sample of 119 asymptomatic adults (age 20-80 years), we evaluated forced vital capacity (FVC) and forced expiratory volume in 1 s (FEV1). We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic nervous system; Spirometry; Smoking.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300702

Relationship of cytokines and cytokine signaling to immunodeficiency disorders in the mouse
Provedor de dados: 56
Autores: Morawetz,R.A.; Giese,N.A.; Gabriele,L.; Rothman,P.; Horak,I.; Ozato,K.; Morse III,H.C.
The contributions of cytokines to the development and progression of disease in a mouse model of retrovirus-induced immunodeficiency (MAIDS) are controversial. Some studies have indicated an etiologic role for type 2 cytokines, while others have emphasized the importance of type 1 cytokines. We have used mice deficient in expression of IL-4, IL-10, IL-4 and IL-10, IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;, or ICSBP - a transcriptional protein involved in IFN signaling - to examine their contributions to this disorder. Our results demonstrate that expression of type 2 cytokines is an epiphenomenon of infection and that IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is a driving force in disease progression. In addition, exogenously administered IL-12...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ICSBP; Immunodeficiency; Interferon; MAIDS.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100008

Protection against cephalosporin-induced lipid peroxidation and nephrotoxicity by (+)-cyanidanol-3 and vitamin E
Provedor de dados: 56
Autores: Cojocel,C.; Tolle,K.-L.; El-Hajj,H.; Baumann,K.
The ability of the clinically used cephalosporins: cephalothin, cefotaxime and cefotiam to induce lipid peroxidation (LPO) and renal damage was compared to that of nephrotoxic cephaloridine under in vivo conditions. Glutathione was measured in rat liver or in renal cortex as non-protein sulfhydryls. LPO was measured in plasma, renal cortex and liver by the generation of malondialdehyde or as the increase in renal cortical concentration of conjugated dienes. Impairment of renal function was measured as the decrease in renal cortical accumulation of the organic anion p-aminohippurate (PAH). Administration of cephalosporins to rats as a single dose (2000 mg/kg, ip) induced a significant glutathione-depletion in the renal cortex with cephaloridine, and in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cephalosporins; Glutathione depletion; Lipid peroxidation; Nephrotoxicity; Antioxidants; Vitamin E.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600017

Chronotype ontogeny related to gender
Provedor de dados: 56
Autores: Duarte,L.L.; Menna-Barreto,L.; Miguel,M.A.L.; Louzada,F.; Araújo,J.; Alam,M.; Areas,R.; Pedrazzoli,M.
Chronotype is an established concept designed to identify distinct phase relationships between the expression of circadian rhythms and external synchronizers in humans. Although it has been widely accepted that chronotype is subjected to ontogenetic modulation, there is no consensus on the interaction between age and gender. This study aimed to determine the relationship between age- and gender-related changes in the morningness-eveningness character in a large sample of people. A total of 14,650 volunteers were asked to complete the Brazilian version of the Horne and Östberg chronotype questionnaire. The data demonstrated that, on average, women were more morning-oriented than men until the age of 30 and there were no significant differences between men...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronotype; Gender; Ontogeny; Circadian rhythms; Sleep timing.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400316

Association between Toll-like receptor 4 Asp299Gly polymorphism and coronary heart disease susceptibility
Provedor de dados: 56
Autores: Wu,B.W.; Zhu,J.; Shi,H.M.; Jin,B.; Wen,Z.C.
Published data on the association between Toll-like receptor 4 (TLR4) Asp299Gly polymorphism and coronary heart disease (CHD) susceptibility are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. English-language studies were identified by searching PubMed and Embase databases (up to November 2016). All epidemiological studies were regarding Caucasians because no TLR4 Asp/Gly and Gly/Gly genotypes have been detected in Asians. A total of 20 case-control studies involving 14,416 cases and 10,764 controls were included in the meta-analysis. Overall, no significant associations were found between TLR4 Asp299Gly polymorphism and CHD susceptibility in the dominant model (OR=0.89; 95%CI=0.74 to 1.06; P=0.20)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toll-like receptor 4; Polymorphism; Coronary heart disease; Meta-analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900609

Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain
Provedor de dados: 56
Autores: Santos,P.R.; Azevedo,M.L.B.; Borges,M.B.J.; Freire,M.S.; Nascimento,J.P.; Moraes,M.T.B.
Measles virus is a highly contagious agent which causes a major health problem in developing countries. The viral genomic RNA is single-stranded, nonsegmented and of negative polarity. Many live attenuated vaccines for measles virus have been developed using either the prototype Edmonston strain or other locally isolated measles strains. Despite the diverse geographic origins of the vaccine viruses and the different attenuation methods used, there was remarkable sequence similarity of H, F and N genes among all vaccine strains. CAM-70 is a Japanese measles attenuated vaccine strain widely used in Brazilian children and produced by Bio-Manguinhos since 1982. Previous studies have characterized this vaccine biologically and genomically. Nevertheless, only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Measles virus; Vaccine; Nucleotide sequence; RNA sequencing.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100005

Evaluation of an anti-carcinoembryonic monoclonal antibody suitable for immunoscintigraphy
Provedor de dados: 56
Autores: Moraes,D.W.; Lima,E.N.P.; Prado,I.B.; Carneiro,C.R.W.
An anti-carcinoembryonic antigen (CEA) monoclonal antibody (mAb 6D1.1) was evaluated in vitro and in vivo to determine its suitability as a tracer for immunoscintigraphy of colorectal carcinomas. Determination of mAb affinity for CEA showed a constant of association of 0.63 ± 0.11 x 109 M-1. Binding of technetium-99m (99mTc)-6D1.1, labeled by a direct method, to human cultured lineages was highly specific. Binding to only CEA-positive LS-174T cells resulted in a saturable curve inhibited by pre-incubation with unlabeled mAb. No binding at all was observed for the human lineages MeWo (melanoma) or ZR75-30 (breast carcinoma), neither of them expressing CEA cells. Intravenous injection of 99mTc-6D1.1 into nude mice xenografted with human LS-174T tumors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monoclonal antibody; Carcinoembryonic antigen; Colorectal carcinoma; Immunoscintigraphy; Technetium-99m.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800006

Human_Leishmaniasis@cytokines.bahia.br
Provedor de dados: 56
Autores: Barral-Netto,M.; Brodskyn,C.; Carvalho,E.M.; Barral,A.
The cell-mediated immune response is critical in the resistance to and recovery from leishmaniasis. Cytokines are central elements in mounting an immune response and have received a great deal of attention in both human and experimental leishmaniasis. IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is responsible for macrophage activation leading to leishmanicidal mechanisms. Understanding the balance of cytokines that lead to enhanced production of or synergize with IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;, and those cytokines that counterbalance its effects is fundamental for developing rational immunotherapeutic or immunoprophylactic approaches to leishmaniasis. Here we focus on the cytokine balance in human leishmaniasis, particularly IL-10 as an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Human leishmaniasis; Immunoregulation; IL-12; IL-10.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100021

Correlation between symptoms of the irritable bowel syndrome and the response to the food extract skin prick test
Provedor de dados: 56
Autores: Soares,R.L.S.; Figueiredo,H.N.; Maneschy,C.P.; Rocha,V.R.S.; Santos,J.M.
The relationship between the irritable bowel syndrome (IBS) and food intolerance is not clear. We studied the cutaneous response to food antigens in 43 volunteers who were students and employees of the Faculty of Medicine of Universidade Federal Fluminense. Subjects were divided into 3 groups after evaluation for Roma II criteria for functional disease of the gastrointestinal tract: group I, 14 volunteers with IBS; group II, 15 volunteers with functional dyspepsia; group III, 14 volunteers without habitual gastrointestinal symptoms. The subjects were submitted to the skin prick test with 9 food antigen extracts, for a total of 387 skin tests (9 per volunteer). Of the 126 tests applied to group I, 24 (19.4%) were positive (a 3-mm wider papule than the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Irritable bowel syndrome; Food intolerance; Skin prick test.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500005

Expression of a hantavirus N protein and its efficacy as antigen in immune assays
Provedor de dados: 56
Autores: Figueiredo,L.T.M.; Moreli,M.L.; Borges,A.A.; Figueiredo,G.G.; Souza,R.L.M.; Aquino,V.H.
Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diagnosis; Hantavirus infection; Araraquara hantavirus; N protein.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700008

Determination of CYP2D6 *3, *4, and *10 frequency in women with breast cancer in São Luís, Brazil, and its association with prognostic factors and disease-free survival
Provedor de dados: 56
Autores: Martins,D.M.F.; Vidal,F.C.B.; Souza,R.D.M.; Brusaca,S.A.; Brito,L.M.O.
The CYP2D6 enzyme is crucial for the metabolism of tamoxifen. The CYP2D6 gene is highly polymorphic, and individuals can be extensive, intermediate, or poor tamoxifen metabolizers. The aim of this study was to determine the frequencies of the CYP2D6 *3, *4, and *10 alleles in women with breast cancer who were treated with tamoxifen and analyze the association of enzyme activity with prognostic factors and disease-free survival. We observed a high frequency of CYP2D6 *10, with an allelic frequency of 0.14 (14.4%). The *3 allele was not present in the studied population, and *4 had an allelic frequency of 0.13 (13.8%). We conclude that patients with reduced CYP2D6 activity did not present worse tumor characteristics or decreased disease-free survival than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tamoxifen; Cytochrome P450 2D6; Breast cancer; Genetic polymorphisms; Prognostic factors; Disease-free survival.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001101008

ADME studies and preliminary safety pharmacology of LDT5, a lead compound for the treatment of benign prostatic hyperplasia
Provedor de dados: 56
Autores: Noël,F.; Nascimento-Viana,J.B.; Romeiro,L.A.S.; Silva,R.O.; Lemes,L.F.N.; Oliveira,A.S.; Giorno,T.B.S.; Fernandes,P.D.; Silva,C.L.M.
This study aimed to estimate the absorption, distribution, metabolism and excretion (ADME) properties and safety of LDT5, a lead compound for oral treatment of benign prostatic hyperplasia that has previously been characterized as a multi-target antagonist of α1A-, α1D-adrenoceptors and 5-HT1A receptors. The preclinical characterization of this compound comprised the evaluation of its in vitro properties, including plasma, microsomal and hepatocytes stability, cytochrome P450 metabolism and inhibition, plasma protein binding, and permeability using MDCK-MDR1 cells. De-risking and preliminary safety pharmacology assays were performed through screening of 44 off-target receptors and in vivo tests in mice (rota-rod and single dose toxicity). LDT5 is stable in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benign prostatic hyperplasia; ADME; Safety; Permeability; CYP; Preclinical development.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200603

Antagonic effect of the inhibition of inducible nitric oxide on the mortality of mice acutely infected with Escherichia coli and Bacteroides fragilis
Provedor de dados: 56
Autores: Astolfi,R.S.; Khouri,D.G.; Brandizzi,L.I.V.; Ávila-Campos,M.J.; Andrade Jr.,H.F. de.
Sepsis, the leading cause of death in intensive care units, is associated with overproduction of nitric oxide (NO) due to inducible NO synthase (iNOS), responsible for some of the pathologic changes. Aminoguanidine (AG) is a selective iNOS inhibitor with reported inconsistent actions in sepsis. To investigate the influence of iNOS, we studied models of acute bacterial sepsis using acute challenges with aerobic (Escherichia coli) and anaerobic (Bacteroides fragilis) bacteria in the presence of AG. Six-week-old, 23 g, male and female BALB/c and C57Bl/6j mice, in equal proportions, were inoculated (ip) with bacteria in groups of 4 animals for each dose and each experiment in the absence or presence of AG (50 mg/kg, ip, starting 24 h before challenge and daily...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Sepsis; Experimental model; Bacterial infections; Escherichia coli; Bacteroides fragilis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300006

Effect of naloxone on food competition aggression in food-restricted high and low aggression pigeons (Columba livia)
Provedor de dados: 56
Autores: Fachinelli,C.; Torrecillas,M.; Rodríguez Echandía,E.L.
We determined the effect of the opiate receptor antagonist naloxone on aggression, emotion, feeder control, and eating behavior in high and low aggression female pigeons maintained at 80% of their normal weight and exposed to food competition interactions. Pigeons were divided into pairs by previously ranked high aggression (total time spent in offensive aggression exceeding 60 s/5 min; N = 6 pairs) and low aggression females (time spent in offensive aggression less than 10 s/5 min; N = 6 pairs). A pigeon in each pair received an sc dose of naloxone (1 mg kg-1 ml saline-1) and the other animal received the vehicle. Trials (10 min) were performed 30 min after the naloxone/vehicle administration. The naloxone group of high aggression pigeons showed lower...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Naloxone; Aggressive behavior; Emotional behavior; Food competition; Pigeons.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300010

Sorafenib prevents liver fibrosis in a non-alcoholic steatohepatitis (NASH) rodent model
Provedor de dados: 56
Autores: Stefano,J.T.; Pereira,I.V.A.; Torres,M.M.; Bida,P.M.; Coelho,A.M.M.; Xerfan,M.P.; Cogliati,B.; Barbeiro,D.F.; Mazo,D.F.C.; Kubrusly,M.S.; D'Albuquerque,L.A.C.; Souza,H.P.; Carrilho,F.J.; Oliveira,C.P.
Liver fibrosis occurring as an outcome of non-alcoholic steatohepatitis (NASH) can precede the development of cirrhosis. We investigated the effects of sorafenib in preventing liver fibrosis in a rodent model of NASH. Adult Sprague-Dawley rats were fed a choline-deficient high-fat diet and exposed to diethylnitrosamine for 6 weeks. The NASH group (n=10) received vehicle and the sorafenib group (n=10) received 2.5 mg·kg-1·day-1 by gavage. A control group (n=4) received only standard diet and vehicle. Following treatment, animals were sacrificed and liver tissue was collected for histologic examination, mRNA isolation, and analysis of mitochondrial function. Genes related to fibrosis (MMP9, TIMP1, TIMP2), oxidative stress (HSP60, HSP90, GST), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NASH; Fibrosis; Mitochondrial dysfunction; Sorafenib.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500408

Effect of blocking Rac1 expression in cholangiocarcinoma QBC939 cells
Provedor de dados: 56
Autores: Xudong,Liu; Guangyi,Wang.
Cholangiocarcinomas (CCs) are malignant tumors that originate from epithelial cells lining the biliary tree and gallbladder. Ras correlative C3 creotoxin substrate 1 (Rac1), a small guanosine triphosphatase, is a critical mediator of various aspects of endothelial cell functions. The objective of the present investigation was to study the effect of blocking Rac1 expression in CCs. Seventy-four extrahepatic CC (ECC) specimens and matched adjacent normal mucosa were obtained from the Department of Pathology, Inner Mongolia Medicine Hospital, between 2007 and 2009. Our results showed that the expression of Rac1 was significantly higher (53.12%) in tumor tissues than in normal tissues. Western blotting data indicated a significant reduction in Rac1-miRNA cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholangiocarcinomas; RNAi; Rac1.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500015

Hemodynamic mechanisms of the attenuated blood pressure response to mental stress after a single bout of maximal dynamic exercise in healthy subjects
Provedor de dados: 56
Autores: Neves,F.J.; Carvalho,A.C.G.; Rocha,N.G.; Silva,B.M.; Sales,A.R.K.; Castro,R.R.T. de; Rocha,J.D.; Thomaz,T.G.; Nóbrega,A.C.L.
To determine the hemodynamic mechanisms responsible for the attenuated blood pressure response to mental stress after exercise, 26 healthy sedentary individuals (age 29 ± 8 years) underwent the Stroop color-word test before and 60 min after a bout of maximal dynamic exercise on a treadmill. A subgroup (N = 11) underwent a time-control experiment without exercise. Blood pressure was continuously and noninvasively recorded by infrared finger photoplethysmography. Stroke volume was derived from pressure signals, and cardiac output and peripheral vascular resistance were calculated. Perceived mental stress scores were comparable between mental stress tests both in the exercise (P = 0.96) and control (P = 0.24) experiments. After exercise, the blood pressure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular system; Blood pressure; Exercise test; Stroop test.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700007

MFG-E8, a clearance glycoprotein of apoptotic cells, as a new marker of disease severity in chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Zhang,S.; Xie,J.G.; Su,B.T.; Li,J.L.; Hu,N.; Chen,J.; Luo,G.W.; Cui,T.P.
Milk fat globule epidermal growth factor 8 (MFG-E8) is an opsonin involved in the phagocytosis of apoptotic cells. In patients with chronic obstructive pulmonary disease (COPD), apoptotic cell clearance is defective. However, whether aberrant MFG-E8 expression is involved in this defect is unknown. In this study, we examined the expression of MFG-E8 in COPD patients. MFG-E8, interleukin (IL)-1β and transforming growth factor (TGF)-β levels were measured in the plasma of 96 COPD patients (93 males, 3 females; age range: 62.12±10.39) and 87 age-matched healthy controls (85 males, 2 females; age range: 64.81±10.11 years) using an enzyme-linked immunosorbent assay. Compared with controls, COPD patients had a significantly lower plasma MFG-E8 levels (P&lt;0.01)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; COPD; MFG-E8; Phagocytosis; Smoking.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101032

Over-expression of cyclooxygenase-2 in endoscopic biopsies of ectopic gastric mucosa
Provedor de dados: 56
Autores: Martins,F.P.; Artigiani Neto,R.; Oshima,C.T.; Costa,P.P. da; Forones,N.M.; Ferrari,A.P.
Ectopic gastric mucosa (EGM) is considered to be a congenital condition. Rare cases of adenocarcinoma have been described. There are no data justifying regular biopsies or follow-up. Cyclooxygenase-2 (COX-2) is a protein involved in gastrointestinal tumor development by inhibiting apoptosis and regulating angiogenesis. The aim of this prospective study was to evaluate COX-2 expression in EGM and compare it with normal tissue and Barrett's esophagus. We evaluated 1327 patients. Biopsies were taken from the inlet patch for histological evaluation and from the gastric antrum to assess Helicobacter pylori infection. Biopsies taken from normal esophageal, gastric antrum and body mucosa and Barrett's esophagus were retrieved from a tissue bank. EGM biopsies were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endoscopy; Ectopic gastric mucosa; Cyclooxygenase-2; Barrett's esophagus; Gastric biopsies.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100005

Clinical and immunological features of patients with atopy and concomitant HTLV-1 infection
Provedor de dados: 56
Autores: Gaspar-Sobrinho,F.P.; Souza-Machado,A.; Santos,S.B.; Orge,G.; Lessa,H.A.; Cruz,A.A.; Carvalho,E.M.
Human T-cell lymphotropic virus type 1 (HTLV-1) induces an exacerbated type 1 immune response characterized by high spontaneous IFN-γ and TNF-α production. Allergic rhinitis and asthma are associated with the type 2 immune response, with elevated secretion of IL-4 and IL-5. The aim of this study was to characterize the immune response in atopic HTLV-1 carriers. The cytokine profile of atopic HTLV-1 carriers (N = 10; all females) was compared with that of non-atopic HTLV-1 carriers (N = 14; 9 females and 5 males). Mean patient age of atopic and non-atopic groups was 45 ± 8 and 38 ± 11 years, respectively. All atopic HTLV-1 carriers had rhinitis with or without asthma and a skin prick test positive for Dermatophagoides pteronyssinus antigen 1 (Derp-1). There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Atopy; Cytokines; HTLV-1; Rhinitis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200005

Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses
Provedor de dados: 56
Autores: Scandalios,J.G.
Molecular oxygen (O2) is the premier biological electron acceptor that serves vital roles in fundamental cellular functions. However, with the beneficial properties of O2 comes the inadvertent formation of reactive oxygen species (ROS) such as superoxide (O2&lt;FONT FACE=Symbol&gt;·-&lt;/FONT&gt;), hydrogen peroxide, and hydroxyl radical (OH&lt;FONT FACE=Symbol&gt;·&lt;/FONT&gt;). If unabated, ROS pose a serious threat to or cause the death of aerobic cells. To minimize the damaging effects of ROS, aerobic organisms evolved non-enzymatic and enzymatic antioxidant defenses. The latter include catalases, peroxidases, superoxide dismutases, and glutathione S-transferases (GST). Cellular ROS-sensing mechanisms are not well understood, but a number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Aging; Telomeres; Gene regulation; Superoxide dismutase; Genomics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700003

FANCD2 Western blot as a diagnostic tool for Brazilian patients with Fanconi anemia
Provedor de dados: 56
Autores: Pilonetto,D.V.; Pereira,N.F.; Bitencourt,M.A.; Magdalena,N.I.R.; Vieira,E.R.; Veiga,L.B.A.; Cavalli,I.J.; Ribeiro,R.C.; Pasquini,R.
Fanconi anemia is a rare hereditary disease showing genetic heterogeneity due to a variety of mutations in genes involved in DNA repair pathways, which may lead to different clinical manifestations. Phenotypic variability makes diagnosis difficult based only on clinical manifestations, therefore laboratory tests are necessary. New advances in molecular pathogenesis of this disease led researchers to develop a diagnostic test based on Western blot for FANCD2. The objective of the present study was to determine the efficacy of this method for the diagnosis of 84 Brazilian patients with Fanconi anemia, all of whom tested positive for the diepoxybutane test, and 98 healthy controls. The FANCD2 monoubiquitinated isoform (FANCDS+/FANCD2L-) was not detected in 77...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anemia; FANCD2 Western blot; Diepoxybutane test; Monoubiquitination; Immunoblotting.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300004

lncRNA MEG3 inhibits the growth of hepatocellular carcinoma cells by sponging miR-9-5p to upregulate SOX11
Provedor de dados: 56
Autores: Liu,Zhi; Chen,Jian Yu; Zhong,Yang; Xie,Liang; Li,Jian Shui.
The long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3), a tumor suppressor, is critical for the carcinogenesis and progression of different cancers, including hepatocellular carcinoma (HCC). To date, the roles of lncRNA MEG3 in HCC are not well illustrated. Therefore, this study used western blot and qRT-PCR to evaluate the expression of MEG3, miR-9-5p, and Sex determining Region Y-related HMG-box 11 (SOX11) in HCC tissues and cell lines. RNA pull-down and luciferase reporter assay were used to evaluate these molecular interactions. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry detected the viability and apoptosis of HCC cells, respectively. The results showed that MEG3 and SOX11 were poorly expressed but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma MEG3; MiR-9-5p SOX11 lncRNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000604

Heart rate changes during the Valsalva maneuver in patients with isolated aortic insufficiency
Provedor de dados: 56
Autores: Navarro,A.E.; Dávila,D.F.; Torres,A.; Bellabarba,G.; Donis,J.H.; Casado,J.
To determine the possible relationship between left ventricular dilatation and heart rate changes provoked by the Valsalva maneuver (Valsalva ratio), we studied 9 patients with isolated chronic aortic insufficiency. Left ventricular systolic function was assessed by two-dimensional echocardiography and cardiac catheterization. All patients were asymptomatic (functional class I of the New York Heart Association). The left ventricular internal diameters and volumes were significantly increased in all patients. The asymptomatic patients had either normal or slightly depressed ejection fraction (EF&amp;gt;0.40). The Valsalva ratio of these asymptomatic patients showed no significant correlation with the left ventricular volumes or with the left ventricular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Valsalva maneuver; Parasympathetic nervous system; Left ventricular systolic function; Chronic aortic insufficiency; Ejection fraction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900005

Tumor growth reduction is regulated at the gene level in Walker 256 tumor-bearing rats supplemented with fish oil rich in EPA and DHA
Provedor de dados: 56
Autores: Borghetti,G.; Yamazaki,R.K.; Coelho,I.; Pequito,D.C.T.; Schiessel,D.L.; Kryczyk,M.; Mamus,R.; Naliwaiko,K.; Fernandes,L.C.
We investigated the effect of fish oil (FO) supplementation on tumor growth, cyclooxygenase 2 (COX-2), peroxisome proliferator-activated receptor gamma (PPARγ), and RelA gene and protein expression in Walker 256 tumor-bearing rats. Male Wistar rats (70 days old) were fed with regular chow (group W) or chow supplemented with 1 g/kg body weight FO daily (group WFO) until they reached 100 days of age. Both groups were then inoculated with a suspension of Walker 256 ascitic tumor cells (3×107 cells/mL). After 14 days the rats were killed, total RNA was isolated from the tumor tissue, and relative mRNA expression was measured using the 2-ΔΔCT method. FO significantly decreased tumor growth (W=13.18±1.58 vsWFO=5.40±0.88 g, P&lt;0.05). FO supplementation also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Fish oil; Gene expression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800696

von Willebrand factor antigen levels in plasma of patients with malignant breast disease
Provedor de dados: 56
Autores: Röhsig,L.M.; Damin,D.C.; Stefani,S.D.; Castro Jr.,C.G.; Roisenberg,I.; Schwartsmann,G.
von Willebrand factor (vWF) is a protein that mediates platelet adherence to the subendothelium during primary hemostasis. High plasma vWF concentrations have been reported in patients with various types of cancer, such as head and neck, laryngeal and prostatic cancer, probably representing an acute phase reactant. In the present study we determined the plasma levels of vWF antigen (vWF:Ag) by quantitative immunoelectrophoresis in 128 female patients with breast cancer as well as in 47 women with benign breast disease and in 27 healthy female controls. The levels of vWF:Ag were 170.7 ± 78 U/dl in patients with cancer, 148.4 ± 59 U/dl in patients with benign disease and 130.6 ± 45 U/dl in controls (P&lt;0.005). We also detected a significant increase in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Von Willebrand factor; Breast cancer; Malignancy.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900004

Necroptosis occurs in osteoblasts during tumor necrosis factor-α stimulation and caspase-8 inhibition
Provedor de dados: 56
Autores: Shi,Guan; Jia,Pu; Chen,Hao; Bao,Li; Feng,Fei; Tang,Hai.
Necroptosis is a regulated cell death mechanism. However, it is unknown whether necroptosis is involved in the death of tumor necrosis factor-α (TNF-α)-treated osteoblasts. Therefore, we conducted the study with TNF-α, Nec-1 (a specific inhibitor of necroptosis), and Z-IETD-FMK (a specific inhibitor of apoptosis) to determine whether necroptosis plays a role in the death of TNF-α-treated osteoblast cell line MC3T3-E1. Cell viability, cell death, and lactate dehydrogenase (LDH) release were assayed to evaluate cytotoxicity. Specific marker proteins receptor interacting protein kinase (RIPK3) and phosphorylated mixed lineage kinase domain-like protein (p-MLKL) for necroptosis, and cleaved caspase 3 for apoptosis were detected by western blot, and mRNA was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Necroptosis; Mouse osteoblast; Z-IETD-FMK; Necrostatin-1; TNF-α.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100603

Participation of cannabinoid receptors in peripheral nociception induced by some NSAIDs
Provedor de dados: 56
Autores: Silva,L.C.R.; Romero,T.R.L.; Guzzo,L.S.; Duarte,I.D.G.
Nonsteroidal anti-inflammatory drugs (NSAIDs) have been used extensively to control inflammatory pain. Several peripheral antinociceptive mechanisms have been described, such as opioid system and NO/cGMP/KATP pathway activation. There is evidence that the cannabinoid system can also contribute to the in vivo pharmacological effects of ibuprofen and indomethacin. However, there is no evidence of the involvement of the endocannabinoid system in the peripheral antinociception induced by NSAIDs. Thus, the aim of this study was to investigate the participation of the endocannabinoid system in the peripheral antinociceptive effect of NSAIDs. All experiments were performed on male Wistar rats (160-200 g; N = 4 per group). Hyperalgesia was induced by a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nonsteroidal anti-inflammatory drugs (NSAIDs); Peripheral antinociception; Cannabinoid system.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200018

Antinociceptive effects of Cremophor EL orally administered to mice
Provedor de dados: 56
Autores: Tabarelli,Z.; Berlese,D.B.; Sauzem,P.D.; Mello,C.F.; Rubin,M.A.
Surfactants are frequently used to improve solubilization of lipophilic drugs. Cremophor EL (CrEL) is a polyoxyethylated castor oil surfactant used to solubilize water-insoluble drugs such as anesthetic, antineoplastic, immunosuppressive and analgesic drugs, vitamins and new synthetic compounds, including potential analgesics. The antinociceptive effect of CrEL (3.2, 6.4 and 10.6 g/kg, in 10 ml/kg body weight, by gavage) on the abdominal writhing response induced by intraperitoneal administration of acetic acid (0.8%, 10 ml/kg body weight) and on the tail immersion test was investigated in mice. Control animals received castor oil (10 ml/kg body weight) or saline (0.9% NaCl, 10 ml/kg body weight). CrEL reduced nociception in a dose-dependent manner in both...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antinociception; Cremophor EL; Tail immersion test; Writhing test; Dipyrone; Morphine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100016

Balance between education- and research-oriented publications from a Brazilian University Hospital
Provedor de dados: 56
Autores: Mendonça de Araujo,K.; Mourão,P.A.S.; Leta,J.
We analyzed the trends of scientific output of the University Hospital, Federal University of Rio de Janeiro. A total of 1420 publications were classified according to pattern and visibility. Most were non-research publications with domestic visibility. With time, there was a tendency to shift from non-research (or education-oriented) publications with domestic visibility to research publications with international visibility. This change may reflect new academic attitudes within the institution concerning the objectives of the hospital and the establishment of scientific research activities. The emphasis of this University Hospital had been on the training of new physicians. However, more recently, the production of new knowledge has been incorporated as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: University Hospital; Scientific publications; Publication visibility; Pattern of publications; Bibliometrics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900001

Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring
Provedor de dados: 56
Autores: Pinheiro,D.F.; Pacheco,P.D.G.; Alvarenga,P.V.; Buratini Jr,J.; Castilho,A.C.S.; Lima,P.F.; Sartori,D.R.S.; Vicentini-Paulino,M.L.M.
This study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 ± 0.1 vs 6.3 ± 0.1 g; mean ± SE) to weaning (42.4 ± 1.3 vs 49.1 ± 1.6 g), although at 16 weeks of age their body weight was similar to control (421.7 ± 8.9 and 428.5 ± 8.5 g). Maternal protein restriction also increased lactase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRNA enzyme abundance; Maternal protein restriction; Disaccharidase activity; Fetal programming.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300287

Prevalence and conditions associated with chronic pelvic pain in women from São Luís, Brazil
Provedor de dados: 56
Autores: Coelho,L.S.C.; Brito,L.M.O.; Chein,M.B.C.; Mascarenhas,T.S.; Costa,J.P.L.; Nogueira,A.A.; Poli-Neto,O.B.
The objective of the present study was to estimate the prevalence of chronic pelvic pain in the community of São Luís, capital of the State of Maranhão, Northeastern Brazil, and to identify independent conditions associated with it. A cross-sectional study was conducted, including a sample of 1470 women older than 14 years predominantly served by the public health system. The interviews were held in the subject's home by trained interviewers not affiliated with the public health services of the municipality. The homes were visited at random according to the city map and the prevalence of the condition was estimated. To identify the associated conditions, the significant variables (P=0.10) were selected and entered in a multivariate analysis model. Data are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic pelvic pain; Prevalence; Dyspareunia; Dysmenorrhea.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900818

Increased sympathetic activity in normotensive offspring of malignant hypertensive parents compared to offspring of normotensive parents
Provedor de dados: 56
Autores: Lopes,H.F.; Consolim-Colombo,F.M.; Barreto-Filho,J.A.S.; Riccio,G.M.G.; Negrão,C.E.; Krieger,E.M.
Malignant hypertension seems to be the consequence of very high blood pressure. Furthermore, an increase in sympathetic and renin-angiotensin system activity is considered to be the main mechanisms producing malignant hypertension. In the present study, 10 offspring of malignant hypertensive (OMH) parents (age 28 ± 5 years, 7 males, 3 females, 2 white and 8 non-white) and 10 offspring of normotensive (ONT) parents (age 28 ± 6 years, 2 males, 8 females, 3 white and 7 non-white) were evaluated. The OMH group had significantly higher (P &lt; 0.05) casual blood pressure (125 ± 10/81 ± 5 mmHg) compared with ONT (99 ± 13/67 ± 5 mmHg). The increase in blood pressure was greater in OMH (Δ SBP = 17 ± 2 vs Δ SBP = 9 ± 1 mmHg in ONT) during cold pressor testing, but...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Malignant hypertension; Offspring of malignant hypertensive parents; Sympathetic activity; Microneurography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000003

A biomechanical study on proximal junctional kyphosis following long-segment posterior spinal fusion
Provedor de dados: 56
Autores: Zhu,Wen-Yi; Zang,Lei; Li,Jian; Guan,Li; Hai,Yong.
Posterior long-segment spinal fusion may lead to proximal junctional kyphosis (PJK). The present study sought to identify the appropriate fusion levels required in order to prevent PJK using finite element analysis. A finite element model was constructed based on the whole-spine computed tomography findings of a healthy adult. Nine commonly used posterior spinal fusion methods were selected. Stress on the annulus fibrosis fibers, the posterior ligamentous complex, and the vertebrae after various spinal fusions in the upright position were compared. This study was divided into two groups: non-fusion and fusion. In the former, the stress between the T10 and the upper thoracic vertebrae was higher. Comparing thoracic and lumbar segments in the fusion group,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adult spinal deformity; Proximal junctional kyphosis; Upper instrumented vertebra; Functional spinal unit; Finite element.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500604

Kinetics of acid-base parameters in conventional hemodialysis
Provedor de dados: 56
Autores: Lugon,J.R.; Pereira,G.R.M.; Strogoff-de-Matos,J.P.; Peixoto,A.J.
Details about the acid-base changes in hemodialysis are scarce in the literature but are potentially relevant to adequate management of patients. We addressed the acid-base kinetics during hemodialysis and throughout the interdialytic period in a cross-sectional study of adults undergoing conventional hemodialysis. Samples for blood gas analysis were obtained from the arterial limb of the arteriovenous fistula before the first session of the week (HD1), immediately at the end of HD1, and on sequential collections at 15, 30, 45, 60, and 120 min post-HD1. Additional blood samples were collected after ∼20 h following the end of the first dialysis and immediately prior to the initiation of the second dialysis of the week. Thirty adult patients were analyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemodialysis; Renal replacement therapy; Kinetics; Adequacy of dialysis; Acid-base.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100611

A link between sleep loss, glucose metabolism and adipokines
Provedor de dados: 56
Autores: Padilha,H.G.; Crispim,C.A.; Zimberg,I.Z.; De-Souza,D.A.; Waterhouse,J.; Tufik,S.; de-Mello,M.T.
The present review evaluates the role of sleep and its alteration in triggering problems of glucose metabolism and the possible involvement of adipokines in this process. A reduction in the amount of time spent sleeping has become an endemic condition in modern society, and a search of the current literature has found important associations between sleep loss and alterations of nutritional and metabolic contexts. Studies suggest that sleep loss is associated with problems in glucose metabolism and a higher risk for the development of insulin resistance and type 2 diabetes mellitus. The mechanism involved may be associated with the decreased efficacy of regulation of the hypothalamus-pituitary-adrenal axis by negative feedback mechanisms in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose metabolism; Insulin resistance; Sleep loss; Adipokines; Type 2 diabetes mellitus; Inflammatory status.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000003

Heat storage rate and acute fatigue in rats
Provedor de dados: 56
Autores: Rodrigues,L.O.C.; Oliveira,A.; Lima,N.R.V.; Machado-Moreira,C.A.
Thermal environmental stress can anticipate acute fatigue during exercise at a fixed intensity (%VO2max). Controversy exists about whether this anticipation is caused by the absolute internal temperature (Tint, ºC), by the heat storage rate (HSR, cal/min) or by both mechanisms. The aim of the present study was to study acute fatigue (total exercise time, TET) during thermal stress by determining Tint and HSR from abdominal temperature. Thermal environmental stress was controlled in an environmental chamber and determined as wet bulb globe temperature (ºC), with three environmental temperatures being studied: cold (18ºC), thermoneutral (23.1ºC) or hot (29.4ºC). Six untrained male Wistar rats weighing 260-360 g were used. The animals were submitted to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thermoregulation; Exercise; Fatigue; Heat storage rate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100018

A semi-automatic computerized method to measure baroreflex-mediated heart rate responses that reduces interobserver variability
Provedor de dados: 56
Autores: Soares,P.P.S.; Ushizima,M.R.; Krieger,E.M.; Irigoyen,M.C.C.
Arterial baroreflex sensitivity estimated by pharmacological impulse stimuli depends on intrinsic signal variability and usually a subjective choice of blood pressure (BP) and heart rate (HR) values. We propose a semi-automatic method to estimate cardiovascular reflex sensitivity to bolus infusions of phenylephrine and nitroprusside. Beat-to-beat BP and HR time series for male Wistar rats (N = 13) were obtained from the digitized signal (sample frequency = 2 kHz) and analyzed by the proposed method (PRM) developed in Matlab language. In the PRM, time series were low-pass filtered with zero-phase distortion (3rd order Butterworth used in the forward and reverse direction) and presented graphically, and parameters were selected interactively. Differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex sensitivity; Heart rate; Blood pressure; Phenylephrine; Nitroprusside.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600018

Fludarabine induces apoptosis in chronic lymphocytic leukemia - the role of P53, Bcl-2, Bax, Mcl-1, and Bag-1 proteins
Provedor de dados: 56
Autores: Faria,J.R.; Yamamoto,M.; Faria,R.M.D.; Kerbauy,J.; Oliveira,J.S.R.
The expression of P53, Bcl-2, Bax, Bag-1, and Mcl-1 proteins in CD5/CD20-positive B-chronic lymphocytic leukemia (B-CLL) cells from 30 typical CLL patients was evaluated before and after 48 h of incubation with 10-6 M fludarabine using multiparametric flow cytometric analysis. Protein expression was correlated with annexin V expression, Rai modified clinical staging, lymphocyte doubling time, and previous treatment. Our main goal was to determine the predictive value of these proteins in CLL cells in terms of disease evolution. Bcl-2 expression decreased from a median fluorescence index (MFI) of 331.71 ± 42.2 to 245.81 ± 52.2 (P &lt; 0.001) after fludarabine treatment, but there was no difference between viable cells (331.57 ± 44.6 MFI) and apoptotic cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic lymphocytic leukemia; Apoptosis; Fludarabine; P53; Bcl-2; Bax; Mcl-1; Bag-1.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300003

Effect of ryanodine on sinus node recovery time determined in vitro
Provedor de dados: 56
Autores: Bassani,J.W.M.; Godoy,C.M.G.; Bassani,R.A.
Evidence has indicated that the sarcoplasmic reticulum (SR) might be involved in the generation of spontaneous electrical activity in atrial pacemaker cells. We report the effect of disabling the SR with ryanodine (0.1 µM) on the sinus node recovery time (SNRT) measured in isolated right atria from 4-6-month-old male Wistar rats. Electrogram and isometric force were recorded at 36.5oC. Two methods for sinus node resetting were used: a) pulse: a single stimulus pulse interpolated at coupling intervals of 50, 65 or 80% of the regular spontaneous cycle length (RCL), and b) train: a 2-min train of pulses at intervals of 50, 65 or 80% of RCL. Corrected SNRT (cSNRT) was calculated as the difference between SNRT (first spontaneous cycle length after stimulation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sinus node recovery time; Overdrive suppression; Electrical stimulation; Calcium; Sarcoplasmic reticulum; Ryanodine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800015

Pentoxifylline decreases tumor necrosis factor and interleukin-1 during high tidal volume
Provedor de dados: 56
Autores: Oliveira-Junior,I.S.; Pinheiro,B.V.; Silva,I.D.C.G.; Salomão,R.; Zollner,R.L.; Beppu,O.S.
Tumor necrosis factor-alpha (TNF-alpha) is one of the most important proinflammatory cytokines which plays a central role in host defense and in the acute inflammatory response related to tissue injury. The major source of TNF-alpha are immune cells such as neutrophils and macrophages. We tested the hypothesis that pentoxifylline, a methylxanthine derivative, down-regulates proinflammatory cytokine expression during acute lung injury in rats. Male Wistar rats weighing 250 to 450 g were anesthetized ip with 50 mg/kg sodium thiopental and randomly divided into three groups: group 1 (N = 7): tidal volume (V T) = 7 ml/kg, respiratory rate (RR) = 50 breaths/min and normal saline infusion; group 2 (N = 7): V T = 42 ml/kg, RR = 9 breaths/min and normal saline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tumor necrosis factor-alpha; Pentoxifylline; Inflammation; Acute lung injury.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000011

The rs878081 polymorphism of AIRE gene increases the risk of rheumatoid arthritis in a Chinese Han population: a case-control study
Provedor de dados: 56
Autores: Yang,Haoyu; Li,Jin; Jiang,Lifeng; Jiang,Xijia; Zhou,Xindie; Xu,Nanwei.
The autoimmune regulator (AIRE), a transcriptional regulator expressed in medullary thymic epithelial cells, plays an important role in thymocyte education and negative selection. Several citations studying the association between the rs878081 exon polymorphism of the AIRE gene and the risk of rheumatoid arthritis (RA) in different populations have yielded conflicting findings. Thus, this case-control study involving 300 RA cases and 300 controls was aimed to identify whether such association existed in a Chinese Han population from East China. The rs878081 polymorphism of the AIRE gene was genotyped. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using the chi-squared test, genetic model analysis, and stratification analysis. Genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIRE; Single-nucleotide polymorphism; Rheumatoid arthritis; Case-control study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200612

Influence of season and pollution on the antioxidant defenses of the cichlid fish acará (Geophagus brasiliensis)
Provedor de dados: 56
Autores: Wilhelm Filho,D.; Torres,M.A.; Tribess,T.B.; Pedrosa,R.C.; Soares,C.H.L.
The livers of Geophagus brasiliensis collected from both a non-polluted site and a polluted site were analyzed for different antioxidant defenses, O2 consumption, thiobarbituric acid-reactive substance (TBARS) levels, and histological damage. Compared to controls (116.6 ± 26.1 nmol g-1), TBARS levels were enhanced at the polluted site (284.2 ± 25.6 nmol g-1), as also was oxygen consumption (86.6 ± 11.3 and 128.5 ± 9.8 µmol O2 min-1 g-1, respectively). With respect to enzymatic antioxidants, increased catalase activities (8.7 ± 1.3 and 29.2 ± 2.4 mmol min-1 g-1, respectively), unchanged superoxide dismutase activities (767.2 ± 113.3 and 563.3 ± 70.2 U g-1, respectively), and diminished glutathione S-transferase activities (29.0 ± 3.2 and 14.9 ± 3.2 µmol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxyradicals; Antioxidant defenses; Fish; Acará; Season; Pollution; Adaptation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600004

Excitatory amino acid receptor blockade within the caudal pressor area and rostral ventrolateral medulla alters cardiovascular responses to nucleus raphe obscurus stimulation in rats
Provedor de dados: 56
Autores: Silva,N.F.; Pires,J.G.P.; Dantas,M.A.; Futuro Neto,H.A.
Pressor responses elicited by stimulation of the nucleus raphe obscurus (NRO) depend on the integrity of the rostral ventrolateral medulla (RVLM). Therefore, to test the participation of excitatory amino acid (EAA) receptors in the cardiovascular responses evoked by NRO stimulation (1 ms, 100 Hz, 40-70 µA, for 10 s), the EAA antagonist kynurenic acid (Kyn) was microinjected at different sites in the ventrolateral medullar surface (2.7 nmol/200 nl) of male Wistar rats (270-320 g, N = 39) and NRO stimulation was repeated. The effects of NRO stimulation were: hypertension (deltaMAP = +43 ± 1 mmHg, P&lt;0.01), bradycardia (deltaHR = -30 ± 7 bpm, P&lt;0.01) and apnea. Bilateral microinjection of Kyn into the RVLM, which did not change baseline parameters,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raphe nuclei; Electrical stimulation; Ventrolateral medulla; Caudal pressor area; Excitatory amino acid receptors; Kynurenic acid; Blood pressure.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000019

Hirsch's index: a case study conducted at the Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo
Provedor de dados: 56
Autores: Torro-Alves,N.; Herculano,R.D.; Terçariol,C.A.S.; Kinouchi Filho,O.; Graeff,C.F.O.
An analysis of scientific bibliographic productivity using the Hirsch h-index, information from the Institute of Scientific Information database and the Curriculum Lattes (CNPq, Brazil) was performed at the Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo (FFCLRP-USP) that has four departments in natural, biological and social sciences. Bibliometric evaluations of undergraduate programs showed a better performance of the departments of Chemistry (P &lt; 0.001) and Biology (P &lt; 0.001) when compared to the departments of Physics and Mathematics and Psychology and Education. We also analyzed the scientific output of the six graduate programs of FFCLRP: Psychology, Psychobiology, Chemistry, Physics Applied to Medicine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H-index; Scientometrics; Science in Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100013

High sensitivity C-reactive protein distribution in the elderly: the Bambuí Cohort Study, Brazil
Provedor de dados: 56
Autores: Assunção,L.G.S.; Eloi-Santos,S.M.; Peixoto,S.V.
The measurement of the serum concentration of the acute-phase reactant C-reactive protein (CRP) provides a useful marker in clinical practice. However, the distribution of CRP is not available for all age and population groups. This study assessed the distribution of high sensitivity-CRP (hs-CRP) by gender and age in 1470 elderly individuals from a Brazilian community that participates in the Bambuí Cohort Study. Blood samples were collected after 12 h of fasting and serum samples were stored at -70°C. Measurements were made with a commercial hs-CRP immunonephelometric instrument. More than 50% of the results were above 3.0 mg/L for both genders. Mean hs-CRP was higher in women (3.62 ± 2.58 mg/L) than in men (3.03 ± 2.50 mg/L). This difference was observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-reactive protein; Elderly; Epidemiology; Cardiovascular risk; Brazil.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200025

Phenylpyrazolone derivatives inhibit gastric emptying in rats by a capsaicin-sensitive afferent pathway
Provedor de dados: 56
Autores: Vinagre,A.M.; Collares,E.F.
Dipyrone (Dp), 4-aminoantipyrine (AA) and antipyrine (At) administered iv and Dp administered icv delay gastric emptying (GE) in rats. The participation of capsaicin (Cps)-sensitive afferent fibers in this phenomenon was evaluated. Male Wistar rats were pretreated sc with Cps (50 mg/kg) or vehicle between the first and second day of life and both groups were submitted to the eye-wiping test. GE was determined in these animals at the age of 8/9 weeks (weight: 200-300 g). Ten minutes before the study, the animals of both groups were treated iv with Dp, AA or At (240 μmol/kg), or saline; or treated icv with Dp (4 μmol/animal) or saline. GE was determined 10 min after treatment by measuring % gastric retention (GR) of saline labeled with phenol red 10 min...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dipyrone; 4-Aminoantipyrine; Antipyrine; Capsaicin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100014

Factors associated with the development of renal complications of diabetes mellitus in São Paulo city
Provedor de dados: 56
The incidence of diabetic end-stage renal failure (ESRF) varies worldwide and risk factors have been demonstrated in several populations. The objective of the present study was to identify possible factors associated with the risk of development of ESRF in patients with diabetes mellitus (DM). Two groups of diabetic subjects were included in a case-control study: 1) one group was submitted to renal replacement therapies, attending dialysis centers in São Paulo city and 2) the same number of controls without clinical nephropathy (two negative dipstick tests for urine protein), matched for duration of DM, were obtained from an outpatient clinic. A standardized questionnaire was used by a single investigator and additional data were obtained from the medical...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chronic renal failure; Diabetes mellitus; Diabetic nephropathy; Associated factors.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600006

Association of exercise training and angiotensin-converting enzyme 2 activator improves baroreflex sensitivity of spontaneously hypertensive rats
Provedor de dados: 56
Autores: Lopes,P.R.; Moreira,M.C.S.; Marques,S.M.; Pinto,I.S.J.; Macedo,L.M.; Silva,C.C.; Freiria-Oliveira,A.H.; Rebelo,A.C.S.; Reis,A.A.S.; Rosa,D.A.; Ferreira-Neto,M.L.; Castro,C.H.; Pedrino,G.R.
The present study sought to determine cardiovascular effects of aerobic training associated with diminazene aceturate (DIZE), an activator of the angiotensin converting enzyme 2, in spontaneously hypertensive rats (SHRs). Male SHRs (280–350 g) were either subjected to exercise training or not (sedentary group). The trained group was subjected to 8 weeks of aerobic training on a treadmill (five times a week, lasting 60 min at an intensity of 50–60% of maximum aerobic speed). In the last 15 days of the experimental protocol, these groups were redistributed into four groups: i) sedentary SHRs with daily treatment of 1 mg/kg DIZE (S+D1); ii) trained SHRs with daily treatment of 1 mg/kg DIZE (T+D1); iii) sedentary SHRs with daily treatment of vehicle (S+V); and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intrinsic heart rate; Diminazene aceturate; Aerobic training; Double autonomic blockade; Renin-angiotensin system.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900603

Absence of peripheral blood mononuclear cells priming in hemodialysis patients
Provedor de dados: 56
Autores: Santos,B.C.; Starobinas,N.; Barbuto,J.A.M.; Russo,M.; Schor,N.
As a consequence of the proinflammatory environment occurring in dialytic patients, cytokine overproduction has been implicated in hemodialysis co-morbidity. However, there are discrepancies among the various studies that have analyzed TNF-alpha synthesis and the presence of peripheral blood mononuclear cell (PBMC) priming in this clinical setting. We measured bioactive cytokine by the L929 cell bioassay, and evaluated PBMC TNF-alpha production by 32 hemodialysis patients (HP) and 51 controls. No difference in TNF-alpha secretion was observed between controls and HP (859 ± 141 vs 697 ± 130 U/10(6) cells). Lipopolysaccharide (5 µg/ml) did not induce any further TNF-alpha release, showing no PBMC priming. Paraformaldehyde-fixed HP PBMC were not cytotoxic to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; Stress response; Translational control; Transcriptional blockage; Priming; Membrane-anchored tumor necrosis factor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200009

Meta-analysis of the association between PADI4 -92C/G polymorphism and rheumatoid arthritis in the Chinese population
Provedor de dados: 56
Autores: Guo,Z.Y.; Zhang,J.X.; Wu,M.; Mei,Y.F.; Lin,X.J.; Bu,C.; Xie,Y.; Wang,J.
Many studies have evaluated the correlation between peptidylarginine deiminase 4 (PADI4) -92C/G polymorphism and rheumatoid arthritis (RA), but the results remain inconclusive. Therefore, we performed a meta-analysis in the Chinese population to provide comprehensive data on the association between PADI4 -92C/G polymorphism and RA. Eligible studies published before May 2016 were identified in PubMed and Chinese databases. The strengths of these associations were assessed by pooled odds ratios (OR) and 95% confidence interval (CI). Eight studies documenting a total of 1351 RA cases and 1585 controls were included in this meta-analysis. In the overall analysis, a significant association between the PADI4 -92C/G polymorphism and RA was found in the Chinese...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meta-analysis; Peptidylarginine deiminase 4; Polymorphism; Rheumatoid arthritis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000605

Roles of monocyte chemotactic protein 1 and nuclear factor-κB in immune response to spinal tuberculosis in a New Zealand white rabbit model
Provedor de dados: 56
Autores: Guo,X.H.; Bai,Z.; Qiang,B.; Bu,F.H.; Zhao,N.
This study aimed to explore the roles of monocyte chemotactic protein 1 (MCP-1) and nuclear factor kappa B (NF-κB) in immune response to spinal tuberculosis in a New Zealand white rabbit model. Forty-eight New Zealand white rabbits were collected and divided into four groups: experimental group (n=30, spinal tuberculosis model was established), the sham group (n=15, sham operation was performed) and the blank group (n=3). The qRT-PCR assay and western blotting were applied to detect the mRNA and protein expressions of MCP-1 and NF-κB in peripheral blood. ELISA was used to measure serum levels of MCP-1, NF-κB, IFN-γ, IL-2, IL-4, and IL-10. Flow cytometry was adopted to assess the distributions of CD4+, CD8+ lymphocytes and CD4+ CD25+ Foxp3 lymphocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocyte chemotactic protein 1; Nuclear factor-κB; Spinal tuberculosis; Immune response.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300602

Extracellular calcium-sensing receptor: structural and functional features and association with diseases
Provedor de dados: 56
Autores: Hauache,O.M.
The recently cloned extracellular calcium-sensing receptor (CaR) is a G protein-coupled receptor that plays an essential role in the regulation of extracellular calcium homeostasis. This receptor is expressed in all tissues related to this control (parathyroid glands, thyroid C-cells, kidneys, intestine and bones) and also in tissues with apparently no role in the maintenance of extracellular calcium levels, such as brain, skin and pancreas. The CaR amino acid sequence is compatible with three major domains: a long and hydrophilic aminoterminal extracellular domain, where most of the activating and inactivating mutations described to date are located and where the dimerization process occurs, and the agonist-binding site is located, a hydrophobic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium-sensing receptor; Familial hypocalciuric hypercalcemia; Autosomal dominant hypoparathyroidism; Calcimimetic drugs; Calcilytic drugs.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500004

Possible etiologies for tropical spastic paraparesis and human T lymphotropic virus I-associated myelopathy
Provedor de dados: 56
Autores: Zaninovic',V.
The epidemiology of tropical spastic paraparesis/human T lymphotropic virus I (HTLV-I)-associated myelopathy (TSP/HAM) is frequently inconsistent and suggests environmental factors in the etiology of these syndromes. The neuropathology corresponds to a toxometabolic or autoimmune process and possibly not to a viral disease. Some logical hypotheses about the etiology and physiopathology of TSP and HAM are proposed. Glutamate-mediated excitotoxicity, central distal axonopathies, cassava, lathyrism and cycad toxicity may explain most cases of TSP. The damage caused to astrocytes and to the blood-brain barrier by HTLV-I plus xenobiotics may explain most cases of HAM. Analysis of the HTLV-I/xenobiotic ratio clarifies most of the paradoxical epidemiology of TSP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TSP/HAM; Paradoxical epidemiology; Toxic and toxoviral etiologies; HTLV-I; Glutamate; Astrocytes; Cycads; Cassava.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100001

Effect of tamoxifen on the coronary vascular reactivity of spontaneously hypertensive female rats
Provedor de dados: 56
Autores: Borgo,M.V.; Lopes,A.B.; Gouvêa,S.A.; Romero,W.G.; Moyses,M.R.; Bissoli,N.S.; Abreu,G.R.
Tamoxifen has been associated with a reduction in the incidence of myocardial infarction. However, the effects of tamoxifen on coronary reactivity have not been fully elucidated. The objective of this study was to determine the effects of chronic treatment with tamoxifen on coronary vascular reactivity in spontaneously hypertensive rats (SHR). Female SHR were divided into four groups (N = 7 each): sham-operated (SHAM), sham-operated and treated with tamoxifen (10 mg/kg) by gavage for 90 days (TAMOX), ovariectomized (OVX), and ovariectomized and treated with tamoxifen (OVX+TAMOX). Mean arterial pressure (MAP), heart rate (HR), coronary perfusion pressure (CPP), and coronary vascular reactivity were measured. MAP and HR were reduced (9.42 and 11.67%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tamoxifen; Estrogen; Hypertension; Menopause; Coronary.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800008

Preventive effect of reduced glutathione on contrast-induced nephropathy in elderly patients undergoing coronary angiography or intervention: a randomized, controlled trial
Provedor de dados: 56
Autores: Jin,B.; Wu,B.W.; Zhang,J.J.; Luo,X.P.; Shi,H.M.
Coronary angiography can be a high-risk condition for the incidence of contrast-induced nephropathy (CIN) in elderly patients. Reduced glutathione, under a variety of mechanisms, may prevent CIN in this procedure. We prospectively examined whether hydration with reduced glutathione is superior to hydration alone for prevention of CIN in an elderly Han Chinese population. A total of 505 patients (271 males and 234 females) aged 75 years or older who underwent non-emergency coronary angiography or an intervention were randomly divided into two groups. The treatment group received hydration with reduced glutathione (n=262) and the control group received hydration alone (n=243). Serum creatinine and blood urea nitrogen levels were measured prior to coronary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast-induced nephropathy Reduced glutathione Randomized; Controlled trial.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900839

The use of stem cells for the treatment of autoimmune diseases
Provedor de dados: 56
Autores: Rosa,S.B.; Voltarelli,J.C.; Chies,J.A.B.; Pranke,P.
Autoimmune diseases constitute a heterogeneous group of conditions commonly treated with anti-inflammatory, immunosuppressant and immunomodulating drugs, with satisfactory results in most cases. Nevertheless, some patients become resistant to conventional therapy. The use of high doses of drugs in such cases results in the need for bone marrow reconstitution, a situation which has stimulated research into the use of hematopoietic stem cells in autoimmune disease therapy. Stem cell transplantation in such diseases aims to destroy the self-reacting immune cells and produce a new functional immune system, as well as substitute cells for tissue damaged in the course of the disease. Significant results, such as the reestablishment of tolerance and a decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoimmune disease; Stem cells; Transplantation; Lupus erythematosus; Arthritis; Type I diabetes mellitus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200001

Autonomic nervous system dysfunction in children with severe tetanus: dissociation of cardiac and vascular sympathetic control
Provedor de dados: 56
Autores: Mazzei de Davila,C.A.; Davila,D.F.; Donis,J.H.; Gonzalo,X.
The medical records of ten pediatric patients with a clinical diagnosis of tetanus were reviewed retrospectively. The heart rate and blood pressure of all tetanus patients were measured noninvasively every hour during the first two weeks of hospitalization. Six of ten tetanus patients presented clinical evidence of sympathetic hyperactivity (group A) and were compared with a control group consisting of four children who required mechanical ventilation for diseases other than tetanus (group B). Heart rate and blood pressure simultaneously and progressively increased to a maximum by day 7. The increase over baseline was 43.70 ± 11.77 bpm (mean ± SD) for heart rate (P&lt;0.01) and 38.60 ± 26.40 mmHg for blood pressure (P&lt;0.01). These values were higher and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tetanus patients; Sympathetic control; Autonomic dysfunction; Blood pressure; Heart rate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600018

Is there an association between T102C polymorphism of the serotonin receptor 2A gene and urinary incontinence?
Provedor de dados: 56
Autores: Schwanke,C.H.A.; Bittencourt,L.; Noronha,J.A.P.; Augustin,S.A.J.; Jung,I.E.; Cruz,I.B.M.
The regulation of bladder function is influenced by central serotonergic modulation. Several genetic polymorphisms related to serotonin control have been described in the literature. T102C polymorphism of the serotonin receptor 2A gene (5-HT2A) has been shown to be associated with certain diseases such as non-fatal acute myocardial infarction, essential hypertension, and alcoholism. In the present study, we examined the association between 5-HT2A gene polymorphism and urinary incontinence in the elderly. A case-control study was performed in 298 elderly community dwellers enrolled in the Gravataí-GENESIS Project, Brazil, which studies gene-environmental interactions in aging and age-related diseases. Clinical, physical, biochemical, and molecular analyses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T102C polymorphism of the serotonin receptor 2A gene; 5-HT2A gene polymorphism; Urinary incontinence; Elderly patients.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000003

Ethanol-induced colitis prevents oral tolerance induction in mice
Provedor de dados: 56
Autores: Andrade,M.C.; Vaz,N.M.; Faria,A.M.C.
The gut mucosa is a major site of contact with antigens from food and microbiota. Usually, these daily contacts with natural antigens do not result in inflammatory reactions; instead they result in a state of systemic hyporesponsiveness named oral tolerance. Inflammatory bowel diseases (IBD) are associated with the breakdown of the immunoregulatory mechanisms that maintain oral tolerance. Several animal models of IBD/colitis are available. In mice, these include targeted disruptions of the genes encoding cytokines, T cell subsets or signaling proteins. Colitis can also be induced by intrarectal administration of chemical substances such as 2,4,6-trinitrobenzene sulfonic acid in 50% ethanol. We report here a novel model of colitis induced by intrarectal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ethanol; Colitis; Oral tolerance; Cytokines.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900013

Peritoneal and serum interleukin-18 levels are not increased in women with minimum or mild endometriosis
Provedor de dados: 56
Autores: Glitz,C.; Souza,C.A.; Rodini,G.P.; Genro,V.; Bilibio,J.P.; Senger,M.; Cunha-Filho,J.S.
Interleukin-18 (IL-18) is a cytokine that belongs to the IL-1 family. Endometriosis is strongly associated with sub-fertility, and affects about 15% of women of reproductive age. IL-18 may favor the progression of endometriosis. The objective of the present study was to determine the concentration of IL-18 in the serum and peritoneal fluid of infertile women with endometriosis. Forty infertile and 25 fertile women were screened in a teaching hospital. Thirty-four infertile patients with minimal or mild endometriosis and 22 fertile controls were enrolled in the study. The primary outcome was the estimate of IL-18 levels and the secondary outcome was the correlation between serum and peritoneal levels of IL-18. There were no differences between the two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Endometriosis; IL-18; Peritoneal fluid; Infertility.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100007

Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001
Provedor de dados: 56
Autores: Miagostovich,M.P.; Santos,F.B. dos; Simone,T.S. de; Costa,E.V.; Filippis,A.M.B.; Schatzmayr,H.G.; Nogueira,R.M.R.
The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dengue viruses type 3; Sequencing; RSS-PCR; Rio de Janeiro; Brazil.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800002

Screening for mutations in human alpha-globin genes by nonradioactive single-strand conformation polymorphism
Provedor de dados: 56
Autores: Jorge,S.B.; Melo,M.B.; Costa,F.F.; Sonati,M.F.
Point mutations and small insertions or deletions in the human alpha-globin genes may produce alpha-chain structural variants and alpha-thalassemia. Mutations can be detected either by direct DNA sequencing or by screening methods, which select the mutated exon for sequencing. Although small (about 1 kb, 3 exons and 2 introns), the alpha-globin genes are duplicate (alpha2 and alpha1) and highy G-C rich, which makes them difficult to denature, reducing sequencing efficiency and causing frequent artifacts. We modified some conditions for PCR and electrophoresis in order to detect mutations in these genes employing nonradioactive single-strand conformation polymorphism (SSCP). Primers previously described by other authors for radioactive SSCP and phast-SSCP...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Alpha-globin genes; Nonradioactive SSCP; Alpha-globin structural variants; Hemoglobinopathies; Mutation screening.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100004

Mutations in the HFE gene and sporadic amyotrophic lateral sclerosis risk: a meta-analysis of observational studies
Provedor de dados: 56
Autores: Li,M.; Wang,L.; Wang,W.; Qi,X.L.; Tang,Z.Y.
Iron homeostasis dysregulation has been regarded as an important mechanism in neurodegenerative diseases. The H63D and C282Y polymorphisms in theHFE gene may be involved in the development of sporadic amyotrophic lateral sclerosis (ALS) through the disruption of iron homeostasis. However, studies investigating the relationship between ALS and these two polymorphisms have yielded contradictory outcomes. We performed a meta-analysis to assess the roles of the H63D and C282Y polymorphisms of HFEin ALS susceptibility. PubMed, MEDLINE, EMBASE, and Cochrane Library databases were systematically searched to identify relevant studies. Strict selection criteria and exclusion criteria were applied. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyotrophic lateral sclerosis; HFE; C282Y; H63D; Polymorphism; Meta-analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300215

Severity score system for progressive myelopathy: development and validation of a new clinical scale
Provedor de dados: 56
Autores: Castilhos,R.M.; Blank,D.; Netto,C.B.O.; Souza,C.F.M.; Fernandes,L.N.T.; Schwartz,I.V.D.; Giugliani,R.; Jardim,L.B.
Progressive myelopathies can be secondary to inborn errors of metabolism (IEM) such as mucopolysaccharidosis, mucolipidosis, and adrenomyeloneuropathy. The available scale, Japanese Orthopaedic Association (JOA) score, was validated only for degenerative vertebral diseases. Our objective is to propose and validate a new scale addressing progressive myelopathies and to present validating data for JOA in these diseases. A new scale, Severity Score System for Progressive Myelopathy (SSPROM), covering motor disability, sphincter dysfunction, spasticity, and sensory losses. Inter- and intra-rater reliabilities were measured. External validation was tested by applying JOA, the Expanded Disability Status Scale (EDSS), the Barthel index, and the Osame Motor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Japanese Orthopaedic Association; Severity Score System for Progressive Myelopathy; Mucopolysaccharidosis; Mucolipidosis; Adrenomyeloneuropathy; Progressive myelopathies.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700001

DNA encoding individual mycobacterial antigens protects mice against tuberculosis
Provedor de dados: 56
Autores: Silva,C.L.; Bonato,V.L.D.; Lima,V.M.F.
Over the last few years, some of our experiments in which mycobacterial antigens were presented to the immune system as if they were viral antigens have had a significant impact on our understanding of protective immunity against tuberculosis. They have also markedly enhanced the prospects for new vaccines. We now know that individual mycobacterial protein antigens can confer protection equal to that from live BCG vaccine in mice. A critical determinant of the outcome of immunization appears to be the degree to which antigen-specific cytotoxic T cells are generated by the immune response. Our most recent studies indicate that DNA vaccination is an effective way to establish long-lasting cytotoxic T cell memory and protection against tuberculosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; DNA vaccination; Cytokines; Th1/Th2.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200012

Obesity and kidney disease: hidden consequences of the epidemic
Provedor de dados: 56
Autores: Kovesdy,C.P.; Furth,S.L.; Zoccali,C.
Obesity has become a worldwide epidemic and its prevalence has been projected to grow by 40% in the next decade. This increasing prevalence has implications for the risk of diabetes, cardiovascular disease and also for chronic kidney disease (CKD). A high body mass index is one of the strongest risk factors for new-onset CKD. In individuals affected by obesity, a compensatory hyperfiltration occurs to meet the heightened metabolic demands of the increased body weight. The increase in intraglomerular pressure can damage the kidneys and raise the risk of developing CKD in the long-term. The incidence of obesity-related glomerulopathy has increased ten-fold in recent years. Obesity has also been shown to be a risk factor for nephrolithiasis, and for a number...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Obesity; Chronic kidney disease; Nephrolithiasis; Kidney cancer; Prevention.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500101

Gastroesophageal reflux episodes in asthmatic patients and their temporal relation with sleep architecture
Provedor de dados: 56
Autores: Mello-Fujita,L.; Roizenblat,S.; Frison.,C.R.; Rodrigues Junior,L.; Garbuio,S.; Tufik,S.; Bittencourt,L.R.A.
Gastroesophageal reflux (GER) is common in asthma patients and can contribute to sleep disruption. The aim of the present study was to determine the time-related distribution of GER events together with their impact on sleep in asthmatic subjects with GER disease symptoms. The inclusion criteria were: 18-65 years, controlled moderate to severe asthma and GER-compatible clinical evidence. The exclusion criteria were: chronic obstructive lung disease, smoking, infections of the upper airways, use of oral corticosteroids, other co-morbidities, pregnancy, sleep-related disorders, night-time shift work, and the use of substances with impact on sleep. Asthmatic patients with nocturnal symptoms were excluded. All-night polysomnography and esophageal pH monitoring...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Gastroesophageal reflux; Polysomnography; Sleep.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200012

Association between ADAM metallopeptidase domain 33 gene polymorphism and risk of childhood asthma: a meta-analysis
Provedor de dados: 56
Autores: Sun,F.J.; Zou,L.Y.; Tong,D.M.; Lu,X.Y.; Li,J.; Deng,C.B.
This study aimed to investigate the association between ADAM metallopeptidase domain 33 (ADAM33) gene polymorphisms and the risk of childhood asthma. The relevant studies about the relationship between ADAM33 gene polymorphisms and childhood asthma were searched from electronic databases and the deadline of retrieval was May 2016. The single nucleotide polymorphisms (SNPs) of ADAM33 (rs511898, rs2280092, rs3918396, rs528557, rs2853209, rs44707, rs2280091 and rs2280089) were analyzed based on several models including the allele, codominant, recessive and dominant models. The results showed that the ADAM33 rs2280091 polymorphism in all four genetic models was associated with an increased risk of childhood asthma. Positive associations were also found between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Childhood; Asthma; ADAM33; Gene polymorphisms; Asthma risk; Meta-analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000609

Quality of life and adherence to treatment in early-treated Brazilian phenylketonuria pediatric patients
Provedor de dados: 56
Autores: Vieira Neto,E.; Maia Filho,H.S.; Monteiro,C.B.; Carvalho,L.M.; Tonon,T.; Vanz,A.P.; Schwartz,I.V.D.; Ribeiro,M.G.
Early dietary treatment of phenylketonuria (PKU), an inborn error of phenylalanine (Phe) metabolism, results in normal cognitive development. Although health-related quality of life (HRQoL) of PKU patients has been reported as unaffected in high-income countries, there are scarce data concerning HRQoL and adherence to treatment of PKU children and adolescents from Brazil. The present study compared HRQoL scores in core dimensions of Brazilian early-treated PKU pediatric patients with those of a reference population, and explored possible relationships between adherence to treatment and HRQoL. Early-treated PKU pediatric patient HRQoL was evaluated by self- and parent-proxy reports of the Pediatric Quality of Life Inventory (PedsQL) core scales. Adherence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenylketonuria; Quality of life; Questionnaires; Patient compliance; Diet therapy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200613

Imbalanced expression of functional surface molecules in regulatory and effector T cells in systemic lupus erythematosus
Provedor de dados: 56
Autores: Mesquita Júnior,D.; Cruvinel,W.M.; Araujo,J.A.P.; Salmazi,K.C.; Kallas,E.G.; Andrade,L.E.C.
Regulatory T (TREG) cells play an important role in maintaining immune tolerance and avoiding autoimmunity. We analyzed the expression of membrane molecules in TREG and effector T cells in systemic lupus erythematosus (SLE). TREG and effector T cells were analyzed for the expression of CTLA-4, PD1, CD28, CD95, GITR, HLA-DR, OX40, CD40L, and CD45RO in 26 patients with active disease, 31 with inactive disease, and 26 healthy controls. TREG cells were defined as CD25+/highCD127Ø/lowFoxP3+, and effector T cells were defined as CD25+CD127+FoxP3Ø. The ratio of TREG to effector T cells expressing GITR, PD1, HLA-DR, OX40, CD40L, and CD45RO was determined in the three groups. The frequency of TREG cells was similar in patients with SLE and controls. However, SLE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; T lymphocytes; Regulatory T cells; Effector T cells.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800662

Immunoexpression of CD95 in chronic gastritis and gastric mucosa-associated lymphomas
Provedor de dados: 56
Autores: Vassallo,J.; Godoy Jr.,C.E.; Godoy,C.E.; Chagas,C.A.; Metze,K.; Trevisan,M.A.S.
CD95 (Fas/APO-1)-mediated apoptosis plays an important role in immunological regulation and is related to the pathogenesis of autoimmune diseases. Immunoexpression of CD95 has been reported to frequently occur in low grade non-Hodgkin lymphomas, especially of post-germinal center histogenesis, among which those originating in mucosa-associated lymphoid tissue (MALT lymphomas). However, there is no report comparing in situ immunoexpression of this marker in lymphomas and the hyperplastic lymphoid reaction (chronic gastritis) related to Helicobacter pylori infection. The purpose of the present research was to compare the intensity of lymphoid CD95 immunoexpression in 15 cases of H. pylori-related chronic gastritis and 15 gastric MALT lymphomas. CD95...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD95; FAS/APO1; Apoptosis; Chronic gastritis; MALT lymphoma.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900015

IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract
Provedor de dados: 56
Autores: Maciel,M.; Fusaro,A.E.; Oliveira,C.R.; Futata,E.A.; Duarte,A.J.S.; Sato,M.N.
Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucosa; Atural IgA antibodies; Oral tolerance; Cytokines; Allergy; Dermatophagoides pteronyssinus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600006

Chronic intermittent exposure to ayahuasca during aging does not affect memory in mice
Provedor de dados: 56
Autores: Correa-Netto,N.F.; Coelho,L.S.; Galfano,G.S.; Nishide,F.; Tamura,F.; Shimizu,M.K.; Santos Junior,J.G.; Linardi,A.
The Quechua term ayahuasca refers to a beverage obtained from decoctions of the liana Banisteriopsis caapi with leaves of Psychotria viridis. The ritualistic use of ayahuasca is becoming a global phenomenon, with some individuals using this beverage throughout life, including in old age. Cognitive impairment is a common manifestation during aging. There are conflicting reports on the ability of some ayahuasca compounds to exert neuroprotective or neurotoxic effects that could improve or impair learning and memory. Animal models provide a relevant and accessible means of investigating the behavioral effects of ayahuasca without the environmental conditions associated with the ritualistic use of the beverage. In this study, we investigated the influence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety Banisteriopsis Carbolines N; N-dimethyltryptamine Learning.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700604

Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats
Provedor de dados: 56
Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 µg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed µg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sertoli cells; Lipids; FSH; Insulin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500004

Cotransfected human chondrocytes: over-expression of IGF-I and SOX9 enhances the synthesis of cartilage matrix components collagen-II and glycosaminoglycans
Provedor de dados: 56
Autores: Simental-Mendía,M.; Lara-Arias,J.; Álvarez-Lozano,E.; Said-Fernández,S.; Soto-Domínguez,A.; Padilla-Rivas,G. R.; Martínez-Rodríguez,H. G.
Damage to cartilage causes a loss of type II collagen (Col-II) and glycosaminoglycans (GAG). To restore the original cartilage architecture, cell factors that stimulate Col-II and GAG production are needed. Insulin-like growth factor I (IGF-I) and transcription factor SOX9 are essential for the synthesis of cartilage matrix, chondrocyte proliferation, and phenotype maintenance. We evaluated the combined effect of IGF-I and SOX9 transgene expression on Col-II and GAG production by cultured human articular chondrocytes. Transient transfection and cotransfection were performed using two mammalian expression plasmids (pCMV-SPORT6), one for each transgene. At day 9 post-transfection, the chondrocytes that were over-expressing IGF-I/SOX9 showed 2-fold increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type II collagen; Glycosaminoglycans; IGF-I/SOX9 transgenes; Human chondrocytes; Articular cartilage; Cotransfection.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201063

Evaluation of a father and son with atypical chronic myeloid leukemia with SETBP1 mutations and a review of the literature
Provedor de dados: 56
Autores: Wang,L.; Du,F.; Zhang,H.-M.; Wang,H.-X.
We report the case of a father and son diagnosed with atypical chronic myeloid leukemia (aCML). Both patients harbored SETBP1 mutations, which are present in 24.3% of aCML patients. Moreover, both shared the variant encoding p.Pro737His, but the aCML severity was greater in the son because of the presence of two other missense mutations causing p.Asp868Asn and p.Ser885Arg alterations. SETBP1 mutations may be associated with an adverse prognosis, so their detection would help in the diagnosis of aCML and the determination of a patient's prognosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atypical chronic myeloid leukemia; SETBP1 mutation; Chronic neutrophilic leukemia; CSF3R mutation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700583

Influence of He-Ne laser therapy on the dynamics of wound healing in mice treated with anti-inflammatory drugs
Provedor de dados: 56
Autores: Gonçalves,W.L.S.; Souza,F.M.; Conti,C.L.; Cirqueira,J.P.; Rocha,W.A.; Pires,J.G.P.; Barros,L.A.P.; Moysés,M.R.
We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in mice treated with steroidal and non-steroidal anti-inflammatory agents. Male albino mice, 28-32 g, were randomized into 6 groups of 6 animals each: control (C), He-Ne laser (L), dexamethasone (D), D + L, celecoxib (X), and X + L. D and X were injected im at doses of 5 and 22 mg/kg, respectively, 24 h before the experiment. A 1-cm long surgical wound was made with a scalpel on the abdomens of the mice. Animals from groups L, D + L and X + L were exposed to 4 J (cm²)-1 day-1 of He-Ne laser for 12 s and were sacrificed on days 1, 2, or 3 after the procedure, when skin samples were taken for histological examination. A significant increase of collagen synthesis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photo-stimulation; Skin; Collagen; Cyclooxygenase; Corticosteroids; Cicatrization; Anti-inflammatory drugs.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600018

Risk factors for glucose intolerance in active acromegaly
Provedor de dados: 56
Autores: Kreze,A.; Kreze-Spirova,E.; Mikulecky,M.
In the present retrospective study we determined the frequency of glucose intolerance in active untreated acromegaly, and searched for risk factors possibly supporting the emergence of the diabetic condition. Among 43 patients, 8 (19%; 95% CI: 8-33%) had diabetes mellitus and 2 (5%; 1-16%) impaired glucose tolerance. No impaired fasting glycemia was demonstrable. The frequency of diabetes was on average 4.5 times higher than in the general Slovak population. Ten factors suspected to support progression to glucose intolerance were studied by comparing the frequency of glucose intolerance between patients with present and absent risk factors. A family history of diabetes and arterial hypertension proved to have a significant promoting effect (P&lt;0.05,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acromegaly; Diabetes mellitus; Impaired glucose tolerance; Impaired fasting glycemia; Risk factors; Inferential statistics.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100009

Protective effects of deferasirox and N-acetyl-L-cysteine on iron overload-injured bone marrow
Provedor de dados: 56
Autores: Shen,J.C.; Zhang,Y.C.; Zhao,M.F.
Using an iron overload mouse model, we explored the protective effect of deferasirox (DFX) and N-acetyl-L-cysteine (NAC) on injured bone marrow hematopoietic stem/progenitor cells (HSPC) induced by iron overload. Mice were intraperitoneally injected with 25 mg iron dextran every 3 days for 4 weeks to establish an iron overload (Fe) model. DFX or NAC were co-administered with iron dextran in two groups of mice (Fe+DFX and Fe+NAC), and the function of HSPCs was then examined. Iron overload markedly decreased the number of murine HSPCs in bone marrow. Subsequent colony-forming cell assays showed that iron overload also decreased the colony forming capacity of HSPCs, the effect of which could be reversed by DFX and NAC. The bone marrow hematopoiesis damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron overload; Bone marrow; Hematopoietic stem/progenitor cells; Deferasirox; N-acetyl-L-cysteine.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200613

Plasma insulin levels are increased by sertraline in rats under oral glucose overload
Provedor de dados: 56
Autores: Gomez,R.; Huber,J.; Lhullier,F.; Barros,H.M.T.
Recognition and control of depression symptoms are important to increase patient compliance with treatment and to improve the quality of life of diabetic patients. Clinical studies indicate that selective serotonin reuptake inhibitors (SSRI) are better antidepressants for diabetic patients than other drugs. However, preclinical trials have demonstrated that not all SSRI reduce plasma glucose levels. In fact, fluoxetine increases and sertraline decreases glycemia in diabetic and non-diabetic rats. In the present study we evaluated plasma insulin levels during fasting and after glucose overload after treatment with sertraline. Adult male Wistar rats were fasted and treated with saline or 30 mg/kg sertraline and submitted or not to glucose overload (N = 10)....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Depression; Antidepressants; Diabetes; Insulinemia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200009

A role for angiogenesis in rheumatoid arthritis
Provedor de dados: 56
Autores: Stupack,D.G.; Storgard,C.M.; Cheresh,D.A.
Rheumatoid arthritis (RA) is a chronic debilitating disease characterized by distinct autoimmune, inflammatory and fibrovascular components which lead to synovial proliferation and joint destruction. However, existing treatments specifically target only autoimmune and inflammatory components despite the fact that neovascularization of the inflamed synovium is a hallmark of rheumatoid arthritis. Angiogenesis may contribute to synovial growth, leukocyte recruitment and tissue remodeling, thus potentiating disease progression. Although no therapies currently target angiogenesis, several existing therapies have anti-angiogenic activity. Recent advances in anti-angiogenic strategies in oncology, including the identification of integrin &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiogenesis; Endothelium; Arthritis; Integrin.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500011

The use of non-human primates as animal models for the study of hepatitis viruses
Provedor de dados: 56
Autores: Vitral,C.L.; Yoshida,C.F.T.; Gaspar,A.M.C.
Hepatitis viruses belong to different families and have in common a striking hepatotropism and restrictions for propagation in cell culture. The transmissibility of hepatitis is in great part limited to non-human primates. Enterically transmitted hepatitis viruses (hepatitis A virus and hepatitis E virus) can induce hepatitis in a number of Old World and New World monkey species, while the host range of non-human primates susceptible to hepatitis viruses transmitted by the parenteral route (hepatitis B virus, hepatitis C virus and hepatitis delta virus) is restricted to few species of Old World monkeys, especially the chimpanzee. Experimental studies on non-human primates have provided an invaluable source of information regarding the biology and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis viruses; Non-human primates; Animal model; Experimental infection.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800003

Introduction to III Forum on the Neurobiology of Stress, Araraquara, SP, Brazil, September 8-10, 2011
Provedor de dados: 56
Autores: Brandão,Marcus Lira.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400001

Lack of evidence for the pathogenic role of iron and HFE gene mutations in Brazilian patients with nonalcoholic steatohepatitis
Provedor de dados: 56
Autores: Deguti,M.M.; Sipahi,A.M.; Gayotto,L.C.C.; Palácios,S.A.; Bittencourt,P.L.; Goldberg,A.C.; Laudanna,A.A.; Carrilho,F.J.; Cançado,E.L.R.
The hypothesis of the role of iron overload associated with HFE gene mutations in the pathogenesis of nonalcoholic steatohepatitis (NASH) has been raised in recent years. In the present study, biochemical and histopathological evidence of iron overload and HFE mutations was investigated in NASH patients. Thirty-two NASH patients, 19 females (59%), average 49.2 years, 72% Caucasians, 12% Mulattoes and 12% Asians, were submitted to serum aminotransferase and iron profile determinations. Liver biopsies were analyzed for necroinflammatory activity, architectural damage and iron deposition. In 31 of the patients, C282Y and H63D mutations were tested by PCR-RFLP. Alanine aminotransferase levels were increased in 30 patients, 2.42 ± 1.12 times the upper normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty liver; Nonalcoholic steatohepatitis; Iron overload; HFE gene; Alanine aminotransferase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600009

Correlation between interleukin-18promoter -607C/A polymorphism and susceptibility to ischemic stroke
Provedor de dados: 56
Autores: Lu,Jian-xia; Lu,Zhong-qian; Zhang,Shao-lan; Zhi,Juan; Chen,Zheng-ping; Wang,Wan-xiang.
Single nucleotide polymorphisms in the promoter region ofinterleukin-18 (IL-18), an inflammatory cytokine, have been linked to susceptibility to many diseases, including cancer and immune dysfunction. Here, we explored the potential association between theIL-18 -607C/A (rs1946518) promoter region polymorphism and susceptibility to ischemic stroke (IS). This locus was amplified from peripheral blood samples of 386 IS patients (cases) and 364 healthy individuals (controls) by the polymerase chain reaction with sequence-specific primers. Significant differences were observed by the χ2 test in the -607C/A (rs1946518) genotype and allele frequencies between cases and controls (P &lt; 0.05). Furthermore, after excluding for age, gender, smoking status, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-18; Polymorphism; Ischemic stroke; Correlation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600502

New strategies in hematopoietic stem cell transplantation: G-CSF-mobilized unprocessed whole blood
Provedor de dados: 56
Autores: Dräger,A.M.; Ossenkoppele,G.J.; Jonkhoff,A.R.; Schuurhuis,G.J.; Huijgens,P.C.
Transplantation of mobilized peripheral blood stem cells (PBSC) for rescue of bone marrow function after high-dose chemo-/radiotherapy is widely used in hematologic malignancies and solid tumors. Mobilization of stem cells to the peripheral blood can be achieved by cytokine treatment of the patients. The main advantage of autologous PBSC transplantation over bone marrow transplantation is the faster recovery of neutrophil and platelet counts. The threshold number of PBSC required for adequate rescue of bone marrow is thought to be about 2 x 106 CD34+ cells/kg, if the stem cells are collected by leukapheresis and subsequently cryopreserved. We show that this critical number could be further reduced to as few as 0.2 x 106 cells/kg. In 30 patients with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoietic stem cells; Transplantation; Cytokines; G-CSF; Lymphoma; Multiple myeloma.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100006

Quality of life on hemodialysis and inflammation: a descriptive analysis
Provedor de dados: 56
Autores: Bacci,M.R.; Adami,F.; Figueiredo,F.W.S.; Alves,B.C.A.; da Veiga,G.L.; Fonseca,F.L.A.
Chronic kidney disease (CKD) is highly prevalent worldwide. Patients with CKD on hemodialysis are more likely to present behavioral changes and worse quality of life as a result of their routine and complications. They also have higher levels of cytokines. The aim of this study is to assess the relationship between the inflammatory profile and quality of life measured by KDOQL-SF36 in hemodialysis outpatients. Patients older than 21 years of age and on routine hemodialysis for at least 6 months with treatment on a regular weekly basis were included and their anthropometric parameters and serum inflammatory markers were evaluated. Thirty patients consented to participate. Homocysteine (Hcy) levels were correlated with worse glomerular filtration rate (GFR;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality of life; Hemodialysis; Inflammation; Homocysteine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600608

The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response
Provedor de dados: 56
Autores: Almeida,S.R.; Lopes,J.D.
In the present study we evaluated T cell proliferation and Th lymphokine patterns in response to gp43 from Paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. T cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. The pattern of T cell lymphokines stimulated by dendritic cells was always Th1, although the levels of IL-2 and IFN-gamma were lower in T cell cultures from susceptible mice. To determie whether different antigen-presenting cells such as macrophages and dendritic cells stimulated different concentrations of Th1 lymphokines, the production of IFN-gamma and IL-2 was measured. It was observed that dendritic cells were more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendritic cells; Paracoccidioidomycosis; Paracoccidioides brasiliensis; Th1/Th2.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400014

Etanercept protects rat cardiomyocytes against hypertrophy by regulating inflammatory cytokines secretion and cell apoptosis
Provedor de dados: 56
Autores: Li,Q.; Yu,Q.; Na,R.; Liu,B.
We aimed to investigate the effect of etanercept, a tumor necrosis factor-α (TNF-α) inhibitor, on rat cardiomyocyte hypertrophy and its underlying mechanism. Primary neonatal rat cardiomyocytes were isolated from Sprague-Dawley rats. The model of rat cardiomyocyte hypertrophy was induced by endothelin, and then treated with different concentrations of etanercept (1, 10, and 50 μM). After treatment, cell counts, viability and cell apoptosis were evaluated. The mRNA levels of myocardial hypertrophy marker genes, including atrial natriuretic factor (ANF), matrix metalloproteinase (MMP)-9 and MMP-13, were detected by qRT-PCR, and the expressions of apoptosis-related proteins (Bcl-2 and Bax) were measured by western blotting. The protein levels of transforming...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tumor necrosis factor-α inhibitor; Endothelin; Myocardial hypertrophy; Cell apoptosis; Inflammatory response.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600604

Induction of a gloverin-like antimicrobial polypeptide in the sugarcane borer Diatraea saccharalis challenged by septic injury
Provedor de dados: 56
Autores: Silva,J.L.C.; Barbosa,J.F.; Bravo,J.P.; Souza,E.M. de; Huergo,L.F.; Pedrosa,F.O.; Esteves,E.; Daffre,S.; Fernandez,M.A.
Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) is an important pest for Brazilian sugarcane. In the present study, we detected two distinct spots in hemolymph from septic injured larvae (HDs1 and HDs2), which are separated by 2DE gel electrophoresis. Both spots were subjected to in-gel tryptic digestion and MALDI-TOF/TOF analysis, which revealed the sequence VFGTLGSDDSGLFGK present in both HDs1 and HDs2. This sequence had homology and 80% identity with specific Lepidoptera antimicrobial peptides called gloverins. Analyses using the ImageMaster 2D software showed pI 8.94 of the HDs1 spot, which is similar to that described to Hyalophora gloveri gloverin (pI 8.5). Moreover, the 14-kDa molecular mass of the spot HDs1 is compatible to that of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diatraea saccharalis; Lepidoptera; Immune response; Gloverin; Sugarcane borer; Hemolymph.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500003

Wheat bran- but not oat bran-enriched diets increase the mucosal height of the cecum and colon of newly weaned and aged rats
Provedor de dados: 56
Autores: Cavaglieri-Felippe,C.R.; Polacow,M.L.O.; Campos,M.R.; Vecchia,M.G.; Curi,R.
The effect of diets enriched with oat or wheat bran (prepared by the addition of 300 g of each fiber to 1000 g of the regular diet), given for 8 weeks, on the mucosal height of the colon and cecum was investigated. Newly weaned (21 days old) and aged (12 months old) male Wistar rats were used in this study. As compared to controls, diets enriched with wheat bran provoked a significant increase in the mucosal height, whereas oat bran did not cause any effect. In newly weaned rats (21 days old), wheat bran increased the mucosal height (µm) in the cecum by 20% (mean ± SEM for 8 rats; 169.1 ± 5.2 and 202.9 ± 8.0 for control and wheat bran, respectively) and in the colon (218.8 ± 7.2 and 264.5 ± 18.8 for control and wheat bran, respectively). A similar effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oat bran; Wheat bran; Colon; Cecum; Mucosa; Ageing.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800016

Anticonvulsant and proconvulsant roles of nitric oxide in experimental epilepsy models
Provedor de dados: 56
Autores: Del-Bel,E.A.; Oliveira,P.R.; Oliveira,J.A.C.; Mishra,P.K.; Jobe,P.C.; Garcia-Cairasco,N.
The effect of acute (120 mg/kg) and chronic (25 mg/kg, twice a day, for 4 days) intraperitonial injection of the nitric oxide (NO) synthase (NOS) inhibitor NG-nitro-L-arginine (L-NOARG) was evaluated on seizure induction by drugs such as pilocarpine and pentylenetetrazole (PTZ) and by sound stimulation of audiogenic seizure-resistant (R) and audiogenic seizure-susceptible (S) rats. Seizures were elicited by a subconvulsant dose of pilocarpine (100 mg/kg) only after NOS inhibition. NOS inhibition also simultaneously potentiated the severity of PTZ-induced limbic seizures (60 mg/kg) and protected against PTZ-induced tonic seizures (80 mg/kg). The audiogenic seizure susceptibility of S or R rats did not change after similar treatments. In conclusion,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental epilepsy; Seizures; Forebrain; Brainstem; Pilocarpine; Pentylenetetrazol; Audiogenic seizures; Genetically epilepsy-prone rats; GEPR; Nitric oxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800010

Effect of JJYMD-C, a novel synthetic derivative of gallic acid, on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro
Provedor de dados: 56
Autores: Xu,G.J.; Lu,Z.H.; Lin,X.; Lin,C.W.; Zheng,L.; Zhao,J.M.
Tissue engineering encapsulated cells such as chondrocytes in the carrier matrix have been widely used to repair cartilage defects. However, chondrocyte phenotype is easily lost when chondrocytes are expanded in vitro by a process defined as “dedifferentiation”. To ensure successful therapy, an effective pro-chondrogenic agent is necessary to overcome the obstacle of limited cell numbers in the restoration process, and dedifferentiation is a prerequisite. Gallic acid (GA) has been used in the treatment of arthritis, but its biocompatibility is inferior to that of other compounds. In this study, we modified GA by incorporating sulfamonomethoxine sodium and synthesized a sulfonamido-based gallate, JJYMD-C, and evaluated its effect on chondrocyte metabolism....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sulfamonomethoxine sodium; Gallic acid; Pro-chondrogenic agent; Chondrocyte; Rabbit articular cartilage; Dedifferentiation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800637

Increased immunoglobulin production in silver catfish (Rhamdia quelen) exposed to agrichemicals
Provedor de dados: 56
Autores: Kreutz,L.C.; Pavan,T.R.; Alves,A.G.; Correia,A.G.; Barriquel,B.; dos Santos,E.D.; Barcellos,L.J.G.
Fish vaccination has been increasingly exploited as a tool to control pathogen infection. The production of immunoglobulin following vaccination might be affected by several factors such as management procedures, water temperature, and the presence of xenobiotics. In the present study, we aimed to investigate the kinetics of immunoglobulin production in silver catfish (Rhamdia quelen) inoculated with inactivated Aeromonas hydrophila and kept at two different water temperatures (17.4±0.4° or 21.3±0.3°C). The effect of a second antigen inoculation and exposure of fish to sublethal concentrations of the herbicides atrazine and glyphosate at 10% of the lethal concentration (LC50-96h) on specific serum antibodies were also investigated. Antibodies to A....
Palavras-chave: Fish; Humoral response; Antibody production; Herbicides; Adjuvant.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600499

Functional mapping of the cardiorespiratory effects of dorsal and median raphe nuclei in the rat
Provedor de dados: 56
Autores: Alvarenga,R.M.; Pires,J.G.P.; Futuro Neto,H.A.
The dorsal (DRN) and median (MRN) raphe nuclei are important sources of serotonergic innervation to the forebrain, projecting to sites involved in cardiovascular regulation. These nuclei have been mapped using electrical stimulation, which has the limitation of stimulating fibers of passage. The present study maps these areas with chemical stimulation, investigating their influence on cardiorespiratory parameters. Urethane-anesthetized (1.2 g/kg, iv) male Wistar rats (280-300 g) were instrumented for pulsatile and mean blood pressure (MBP), heart rate, renal nerve activity, and respiratory frequency recordings. Microinjections of L-glutamate (0.18 M, 50-100 nl with 1% Pontamine Sky Blue) were performed within the DRN or the MRN with glass micropipettes. At...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dorsal raphe nucleus; Median raphe nucleus; L-glutamate; Blood pressure; Respiration; Sympathetic nerve activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100022

Dermatan sulfate reduces monocyte chemoattractant protein 1 and TGF-β production, as well as macrophage recruitment and myofibroblast accumulation in mice with unilateral ureteral obstruction
Provedor de dados: 56
Autores: Belmiro,C.L.R.; Gonçalves,R.G.; Kozlowski,E.O.; Werneck,A.F.; Takyia,C.M.; Leite-Jr.,M.; Pavão,M.S.G.
Selectins play an essential role in most inflammatory reactions, mediating the initial leukocyte-rolling event on activated endothelium. Heparin and dermatan sulfate (DS) bind and block P- and L-selectin function in vitro. Recently, we reported that subcutaneous administration of DS inhibits colon inflammation in rats by reducing macrophage and T-cell recruitment and macrophage activation. In the present study, we examined the effect of porcine intestinal mucosa DS on renal inflammation and fibrosis in mice after unilateral ureteral obstruction (UUO). Twenty-four adult male Swiss mice weighing 20-25 g were divided into 4 groups: group C (N = 6) was not subjected to any surgical manipulation; group SH (N = 6) was subjected to surgical manipulation but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycosaminoglycans; Dermatan sulfate; Kidney; Inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700003

Effect of calcium intake on urinary oxalate excretion in calcium stone-forming patients
Provedor de dados: 56
Autores: Nishiura,J.L.; Martini,L.A.; Mendonça,C.O.G.; Schor,N.; Heilberg,I.P.
Dietary calcium lowers the risk of nephrolithiasis due to a decreased absorption of dietary oxalate that is bound by intestinal calcium. The aim of the present study was to evaluate oxaluria in normocalciuric and hypercalciuric lithiasic patients under different calcium intake. Fifty patients (26 females and 24 males, 41 ± 10 years old), whose 4-day dietary records revealed a regular low calcium intake (&lt;=500 mg/day), received an oral calcium load (1 g/day) for 7 days. A 24-h urine was obtained before and after load and according to the calciuria under both diets, patients were considered as normocalciuric (NC, N = 15), diet-dependent hypercalciuric (DDHC, N = 9) or diet-independent hypercalciuric (DIHC, N = 26). On regular diet, mean oxaluria was 30 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Hypercalciuria; Hyperoxaluria; Kidney stones; Oxalate.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600006

Effect of intraoperative lung-protective mechanical ventilation on pulmonary oxygenation function and postoperative pulmonary complications after laparoscopic radical gastrectomy
Provedor de dados: 56
Autores: Liu,Jing; Meng,Zhipeng; lv,Ran; Zhang,Yaping; Wang,Gaojian; Xie,Junran.
This study aimed to observe the effects of lung-protective ventilation (LPV) on oxygenation index (OI) and postoperative pulmonary complications (PPCs) after laparoscopic radical gastrectomy in middle-aged and elderly patients. A total of 120 patients who were scheduled to undergo laparoscopic radical gastrectomy with an expected time of &gt;3 h were randomly divided into conventional ventilation (CV group) with tidal volume (TV) of 10 mL/kg without positive end-expiratory pressure (PEEP), and lung-protective ventilation (PV group) with 7 mL/kg TV and personal level of PEEP with regular recruitment maneuver every 30 min. Measurements of OI, modified clinical pulmonary infection score (mCPIS), and PPCs were assessed during the perioperative period....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung-protective ventilation strategy; Laparoscopic radical gastrectomy; Middle-aged and elderly people; Lung oxygenation function; Postoperative pulmonary complication.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600611

Salt and insulin sensitivity after short and prolonged high salt intake in elderly subjects
Provedor de dados: 56
Autores: Lima,N.K.C.; Tozetto,D.J.O.; Lima,L.G.; Nobre,F.; Moriguti,J.C.; Ferriolli,E.; Foss,M.C.
Salt sensitivity and insulin resistance are correlated with higher cardiovascular risk. There is no information about changes in salt sensitivity (SS) and insulin sensitivity (IS) after a chronic salt overload in humans. The aim of this study was to evaluate these parameters in the elderly. Seventeen volunteers aged 70.5 ± 5.9 years followed a low-salt diet (LSD) for 1 week and a high-salt diet (HSD) for 13 weeks. We evaluated SS after one week (HSD1) and after 13 weeks (HSD13), and subjects’ IS and lipids on their usual diet (UD) at HSD1, and at HSD13. Blood pressure (BP) was measured at each visit and ambulatory blood pressure monitoring (ABPM) was performed twice. SS was the same at HSD1 and HSD13. Systolic BP was lower on LSD than on UD (P = 0.01),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salt sensitivity; Insulin sensitivity; Elderly; Ambulatory blood pressure monitoring; Blood pressure.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800008

Aerobic training modulates T cell activation in elderly women with knee osteoarthritis
Provedor de dados: 56
Autores: Gomes,W.F.; Lacerda,A.C.R.; Brito-Melo,G.E.A.; Fonseca,S.F.; Rocha-Vieira,E.; Leopoldino,A.A.O.; Amorim,M.R.; Mendonça,V.A.
Osteoarthritis of the knee (kOA) is a disease that mainly affects the elderly and can lead to major physical and functional limitations. However, the specific effects of walking, particularly on the immune system, are unknown. Therefore, this study aimed to analyze the effect of 12 weeks of walking (3×/week) on the leukocyte profile and quality of life (QL) of elderly women with kOA. Sixteen women (age: 67±4 years, body mass index: 28.07±4.16 kg/m2) participated in a walking program. The variables were assessed before and after 12 weeks of training with a progressively longer duration (30–55 min) and higher intensity (72–82% of HRmax determined using a graded incremental treadmill test). The QL was assessed using the Medical Outcomes Study 36-Item Short...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoarthritis; Elderly; Exercise; CD4-positive T-lymphocytes; CD8-positive T-lymphocytes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100705

Synergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets
Provedor de dados: 56
We studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.8 mM glucose) was similar in control and PRL-treated islets (1.84 ± 0.06% and 2.08 ± 0.07% of the islet insulin content, respectively). At 5.6 and 22 mM glucose, insulin secretion was significantly higher in PRL-treated than in control islets, achieving 1.38 ± 0.15% and 3.09 ± 0.21% of the islet insulin content in control and 2.43 ± 0.16% and 4.31 ± 0.24% of the islet insulin content in PRL-treated islets, respectively. The expression of the glucose transporter GLUT2 in B-cell membranes was dose-dependently increased by exposure of the islet to increasing glucose...
Palavras-chave: Pancreatic islets; Glucose transporter; Prolactin; Glucose.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300008

The Lebanese mutation as an important cause of familial hypercholesterolemia in Brazil
Provedor de dados: 56
Autores: Alberto,F.L.; Figueiredo,M.S.; Zago,M.A.; Araújo,A.G.; Dos-Santos,J.E.
Familial hypercholesterolemia (FH) is a common autosomal disorder that affects about one in 500 individuals in most Western populations and is caused by a defect in the low-density-lipoprotein receptor (LDLr) gene. In this report we determined the molecular basis of FH in 59 patients from 31 unrelated Brazilian families. All patients were screened for the Lebanese mutation, gross abnormalities of the LDLr gene, and the point mutation in the codon 3500 of the apolipoprotein B-100 gene. None of the 59 patients presented the apoB-3500 mutation, suggesting that familial defective ApoB-100 (FDB) is not a major cause of inherited hypercholesterolemia in Brazil. A novel 4-kb deletion in the LDLr gene, spanning from intron 12 to intron 14, was characterized in one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Familial hypercholesterolemia; LDL receptor gene; Lebanese mutation; Haplotype analysis; ApoB-100 gene; Alu sequences.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600009

Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway
Provedor de dados: 56
Autores: Xiangming,Xu; Yun,Qian; Guoliang,Zhang; Jianjiang,Lin; Lisong,Teng.
The epithelial-mesenchymal transition (EMT) is involved in neoplastic metastasis, and the RON protein may be involved. In the present study, we determined the role and the mechanisms of action of RON in EMT in Madin-Darby canine kidney (MDCK) cells by Western blot and cell migration analysis. Activation of RON by macrophage stimulating protein (MSP) results in cell migration and initiates changes in the morphology of RON-cDNA-transfected MDCK cells. The absence of E-cadherin, the presence of vimentin and an increase in Snail were observed in RE7 cells, which were derived from MDCK cells transfected with wt-RON, compared with MDCK cells. Stimulation of RE7 cells with MSP resulted in increased migration (about 69% of the wounded areas were covered) as well...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RON; Epithelial-mesenchymal transition; MAPK; Erk.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700004

Characterization of nerve-induced relaxation of gastrointestinal sphincteric smooth muscle in a South American opossum (Didelphis albiventris)
Provedor de dados: 56
The presence of inhibitory nonadrenergic noncholinergic (NANC) intrinsic innervation of the circular muscle of the gastrointestinal sphincters of the South American (SA) opossum was investigated in vitro. Isolated circular muscle strips from the esophagogastric and ileocolonic junctions but not from the gastroduodenal (pylorus) region developed spontaneous tension. Tetrodotoxin (TTX, 1 µM) augmented the spontaneous tension only in the ileocolonic junction strips. Electrical field stimulation of esophagogastric and ileocolonic junction strips caused frequency-dependent responses consisting of a relaxation at lower frequencies (&amp;lt;1 Hz) and a biphasic response or contraction at higher frequencies. In the strips from the pyloric region electrical field...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nonadrenergic noncholinergic nerves; Gastrointestinal sphincter relaxation; South American opossum.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600015

An extract of Polygonum multiflorum protects against free radical damage induced by ultraviolet B irradiation of the skin
Provedor de dados: 56
Autores: Hwang,I.K.; Yoo,K.-Y.; Kim,D.W.; Jeong,S.J.; Won,C.-K.; Moon,W.-K.; Kim,Y.S.; Kwon,D.Y.; Won,M.H.; Kim,D.-W.
Over the last decades, the incidence of ultraviolet B (UVB)-related skin problems has been increasing. Damages induced by UVB radiation are related to mutations that occur as a result of direct DNA damage and/or the production of reactive oxygen species. We investigated the anti-oxidant effects of a Polygonum multiflorum thumb extract against skin damage induced by UVB irradiation. Female SKH-1 hairless mice were divided into three groups: control (N = 7), distilled water- (N = 10), and P. multiflorum extract-treated (PM, N = 10) groups. The PM (10 g) was extracted with 100 mL distilled water, cryo-dried and 9.8 g was obtained. The animals received a topical application of 500 µL distilled water or PM extract (1, 2, 4, 8, and 16%, w/v, dissolved in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polygonum multiflorum extract Skin Ultraviolet B irradiation Free radicals Cu; Zn-superoxide dismutase Hairless mouse.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900005

Molecular analysis of CIB4 gene and protein in Kermani sheep
Provedor de dados: 56
Autores: Mohammadabadi,M.R.; Jafari,A.H.D.; Bordbar,F.
The human calcium- and integrin-binding protein (CIB) family is composed of CIB1, CIB2, CIB3, and CIB4 proteins and the CIB4 gene affects fertility. Kermani sheep is one of the most important breeds of Iranian sheep breeds. The aim of this study was to analyze for the first time molecular characteristics of the CIB4 gene and protein in Kermani sheep. Different tissues were collected from the Kermani sheep and real time PCR was performed. The PCR products were sequenced, comparative analyses of the nucleotide sequences were performed, a phylogenetic tree was constructed, and different characteristics of CIB4 proteins were predicted. Real time PCR results showed that the CIB4 gene is expressed only in testis of Kermani sheep. The cDNA nucleotide sequence was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CIB4 gene; Kermani sheep; Molecular characteristics; Testis; Interaction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100602

Effects of dietary lipids on renal function of aged rats
Provedor de dados: 56
Autores: Valente Gamba,C.; Zeraib Caraviello,A.; Matsushita,A.; Alves,G.M.; Nunes da Silva,L.; Nascimento Gomes,G.; Zaladek Gil,F.
Normal aging is accompanied by renal functional and morphological deterioration and dietetic manipulation has been used to delay this age-related decline. We examined the effects of chronic administration of diets containing 5% lipid-enriched diet (LD, w/w) on renal function of rats at different ages. Three types of LD were tested: canola oil, fish oil and butter. Mean systemic tail-cuff blood pressure and glycemia remained within the normal range whatever the age and the diet of the animals. Proteinuria began to rise from the 8th month in the groups ingesting LD, while in the control group it increased significantly (above 10 mg/24 h) only after the 10th month. With age, a significant and progressive decline in glomerular filtration rate (GFR) and renal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aging; Lipids; Proteinuria; Renal function; Acid excretion; Canola oil; Fish oil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200016

Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways
Provedor de dados: 56
Autores: Yang,Zheng; Zhang,Hui; An,Ming; Bian,Mengni; Song,Miao; Guo,Xiaohua; Liu,Quanli; Qiu,Min.
Total Panax notoginseng saponin (TPNS) is the main bioactivity compound derived from the roots and rhizomes of Panax notoginseng (Burk.) F.H. Chen. The aim of this study was to investigate the effectiveness of TPNS in treating vascular neointimal hyperplasia in rats and its mechanisms. Male Sprague-Dawley rats were randomly divided into five groups, sham (control), injury, and low, medium, and high dose TPNS (5, 10, and 20 mg/kg). An in vivo 2F Fogarty balloon-induced carotid artery injury model was established in rats. TPNS significantly and dose-dependently reduced balloon injury-induced neointimal area (NIA) (P&lt;0.001, for all doses) and NIA/media area (MA) (P&lt;0.030, for all doses) in the carotid artery of rats, and PCNA expression (P&lt;0.001,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total Panax notoginseng saponin; Neointimal hyperplasia; Vascular injury; Phosphorylated extracellular signal-regulated kinase1/2; P38 mitogen-activated protein kinase.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100606

A leucine-supplemented diet improved protein content of skeletal muscle in young tumor-bearing rats
Provedor de dados: 56
Autores: Gomes-Marcondes,M.C.C.; Ventrucci,G.; Toledo,M.T.; Cury,L.; Cooper,J.C.
Cancer cachexia induces host protein wastage but the mechanisms are poorly understood. Branched-chain amino acids play a regulatory role in the modulation of both protein synthesis and degradation in host tissues. Leucine, an important amino acid in skeletal muscle, is higher oxidized in tumor-bearing animals. A leucine-supplemented diet was used to analyze the effects of Walker 256 tumor growth on body composition in young weanling Wistar rats divided into two main dietary groups: normal diet (N, 18% protein) and leucine-rich diet (L, 15% protein plus 3% leucine), which were further subdivided into control (N or L) or tumor-bearing (W or LW) subgroups. After 12 days, the animals were sacrificed and their carcass analyzed. The tumor-bearing groups showed a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leucine; Amino acid; Walker 256 carcinosarcoma; Body composition; Skeletal muscle protein.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100017

Immunological properties of gene vaccines delivered by different routes
Provedor de dados: 56
Autores: Oliveira,S.C.; Rosinha,G.M.S.; de-Brito,C.F.A.; Fonseca,C.T.; Afonso,R.R.; Costa,M.C.M.S.; Goes,A.M.; Rech,E.L.; Azevedo,V.
Gene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene vaccination; Naked DNA; Gene gun; Cytokines; Immune response.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200009

Evaluation of genotype resistance testing for salvage antiretroviral therapy at AIDS care centers from Ribeirão Preto, São Paulo, Brazil
Provedor de dados: 56
Autores: Pires Neto,R.J.; Colares,J.K.B.; Fonseca,B.A.L.
The availability of HIV-1 genotype resistance testing (GRT) to clinicians has been insufficiently studied outside randomized clinical trials. The present study evaluated the outcome of salvage antiretroviral therapy (ART) recommended by an expert physician based on GRT in a non-clinical trial setting in Ribeirão Preto, Brazil. A prospective, open, nonrandomized study evaluating easy access to GRT at six Brazilian AIDS Clinics was carried out. This cooperative study analyzed the efficacy of treatment recommended to patients whose salvage ART was guided by GRT with that of treatment with ART based only on previous ART history. A total of 112 patients with ART failure were included in the study, and 77 of them were submitted to GRT. The median CD4 cell count...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Antiretroviral therapy; Salvage therapy; HIV-1 genotype resistance testing.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600016

Development of the endocrine pancreas and novel strategies for β-cell mass restoration and diabetes therapy
Provedor de dados: 56
Autores: Márquez-Aguirre,A.L.; Canales-Aguirre,A.A.; Padilla-Camberos,E.; Esquivel-Solis,H.; Díaz-Martínez,N.E.
Diabetes mellitus represents a serious public health problem owing to its global prevalence in the last decade. The causes of this metabolic disease include dysfunction and/or insufficient number of β cells. Existing diabetes mellitus treatments do not reverse or control the disease. Therefore, β-cell mass restoration might be a promising treatment. Several restoration approaches have been developed: inducing the proliferation of remaining insulin-producing cells, de novo islet formation from pancreatic progenitor cells (neogenesis), and converting non-β cells within the pancreas to β cells (transdifferentiation) are the most direct, simple, and least invasive ways to increase β-cell mass. However, their clinical significance is yet to be determined....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocrine pancreas; Diabetes mellitus; Β-cell regeneration; Β-cell replacement.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900765

Evaluation of the cholinomimetic actions of trimethylsulfonium, a compound present in the midgut gland of the sea hare Aplysia brasiliana (Gastropoda, Opisthobranchia)
Provedor de dados: 56
Autores: Kerchove,C.M.; Markus,R.P.; Freitas,J.C.; Costa-Lotufo,L.V.
Trimethylsulfonium, a compound present in the midgut gland of the sea hare Aplysia brasiliana, negatively modulates vagal response, indicating a probable ability to inhibit cholinergic responses. In the present study, the pharmacological profile of trimethylsulfonium was characterized on muscarinic and nicotinic acetylcholine receptors. In rat jejunum the contractile response induced by trimethylsulfonium (pD2 = 2.46 ± 0.12 and maximal response = 2.14 ± 0.32 g) was not antagonized competitively by atropine. The maximal response (Emax) to trimethylsulfonium was diminished in the presence of increasing doses of atropine (P&lt;0.05), suggesting that trimethylsulfonium-induced contraction was not related to muscarinic stimulation, but might be caused by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aplysia brasiliana; Trimethylsulfonium; Cholinergic systems; Muscarinic acetylcholine receptors; Nicotinic acetylcholine receptors.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400013

HBV vaccination of HCV-infected patients with occult HBV infection and anti-HBc-positive blood donors
Provedor de dados: 56
Autores: Pereira,J.S.F.; Gonçales,N.S.L.; Silva,C.; Lazarini,M.S.K.; Pavan,M.H.P.; Fais,V.C.; Gonçales Júnior,F.L.
Anti-HBc positivity is a frequent cause of donation rejection at blood banks. Hepatitis B virus (HBV) infection may also occur in HBsAg-negative patients, a situation denoted occult infection. Similarly, very low levels of HBV-DNA have also been found in the sera of patients with chronic hepatitis C virus (HCV) infection, even in the absence of serum HBsAg. Initially we searched for HBV-DNA in serum of 100 blood donors and 50 HCV-infected patients who were HBsAg negative/anti-HBc positive by nested-PCR and by an HBV monitor commercial test for HBV-DNA. Anti-HBs seroconversion rates were measured in 100 blood donors and in 22 patients with chronic HCV infection after HBV vaccination to determine if the HBV vaccination could eliminate an occult HBV infection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Anti-HBc-positive patients; HCV co-infection; HBV vaccination; Occult HBV infection.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400013

Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
Provedor de dados: 56
Autores: Ribeiro,L.P.; Freitas-Lima,L.C.; Naumann,G.B.; Meyrelles,S.S.; Lunz,W.; Pires,S.F.; Andrade,H.M.; Carnielli,J.B.T.; Figueiredo,S.G.
In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inborn aerobic capacity; Proteomics; 2-DE; MS; Heart proteins.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300609

The hyperglycemia induced by angiotensin II in rats is mediated by AT1 receptors
Provedor de dados: 56
Autores: Machado,L.J.C.; Marubayashi,U.; Reis,A.M.; Coimbra,C.C.
We have shown that the renin-angiotensin system (RAS) is involved in glucose homeostasis during acute hemorrhage. Since almost all of the physiological actions described for angiotensin II were mediated by AT1 receptors, the present experiments were designed to determine the participation of AT1 receptors in the hyperglycemic action of angiotensin II in freely moving rats. The animals were divided into two experimental groups: 1) animals submitted to intravenous administration of angiotensin II (0.96 nmol/100 g body weight) which caused a rapid increase in plasma glucose reaching the highest values at 5 min after the injection (33% of the initial values, P&amp;lt;0.01), and 2) animals submitted to intravenous administration of DuP-753 (losartan), a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glycemia; Angiotensin II; DuP-753; AT1; Receptor.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000018

MSX1 gene and nonsyndromic oral clefts in a Southern Brazilian population
Provedor de dados: 56
Autores: Souza,L.T.; Kowalski,T.W.; Collares,M.V.M.; Félix,T.M.
Nonsyndromic oral clefts (NSOC) are the most common craniofacial birth defects in humans. The etiology of NSOC is complex, involving both genetic and environmental factors. Several genes that play a role in cellular proliferation, differentiation, and apoptosis have been associated with clefting. For example, variations in the homeobox gene family member MSX1, including a CA repeat located within its single intron, may play a role in clefting. The aim of this study was to investigate the association between MSX1CA repeat polymorphism and NSOC in a Southern Brazilian population using a case-parent triad design. We studied 182 nuclear families with NSOC recruited from the Hospital de Clínicas de Porto Alegre in Southern Brazil. The polymorphic region was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MSX1; Cleft lip and palate; Oral clefts.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700555

Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine
Provedor de dados: 56
Autores: van der Poel,W.H.M.; Pavio,N.; van der Goot,J.; van Es,M.; Martin,M.; Engel,B.
Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 ELISA, a recombinant baculovirus expression product of HEV Gt3 open reading frame-2 was produced and coated onto polystyrene ELISA plates. After incubation of porcine sera, bound HEV antibodies were detected with anti-porcine anti-IgG and anti-IgM conjugates. For primary estimation of sensitivity and specificity of the assay, sets of sera were used from pigs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis E virus; Zoonosis; Swine; ELISA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400334

Cytotoxicity and antitumoral activity of dichloromethane extract and its fractions from Pothomorphe umbellata
Provedor de dados: 56
Autores: Sacoman,J.L.; Monteiro,K.M.; Possenti,A.; Figueira,G.M.; Foglio,M.A.; Carvalho,J.E.
The cytotoxicity of the dichloromethane crude extract (DCE), obtained from the aerial parts of Pothomorphe umbellata (L.) Miq (Piperaceae), was evaluated against nine human cancer cell lines (MCF-7, NCI-ADR/RES, OVCAR-3, PC-3, HT-29, NCI-H460, 786-O, UACC-62, K-562). The DCE presented antiproliferative activity with good potency against all cell lines at low concentrations (between 4.0 and 9.5 µg/mL) and with selectivity (1.55 µg/mL) for the leukemia cell line (K-652). DCE (100, 200, 300 and 400 mg/kg, ip) was also evaluated in the Ehrlich ascites tumor model. Both the survival number and the life span of the animals that died increased by at least 45 and 50%, respectively (8 animals per group), demonstrating P. umbellata extract potential anticancer...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pothomorphe umbellata; Piperaceae; Antitumor activity; Ehrlich ascites tumor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500010

Mechanisms of endothelial dysfunction in obesity-associated hypertension
Provedor de dados: 56
Autores: Lobato,N.S.; Filgueira,F.P.; Akamine,E.H.; Tostes,R.C.; Carvalho,M.H.C.; Fortes,Z.B.
Obesity is strongly associated with high blood pressure, dyslipidemia, and type 2 diabetes. These conditions synergistically increase the risk of cardiovascular events. A number of central and peripheral abnormalities can explain the development or maintenance of high blood pressure in obesity. Of great interest is endothelial dysfunction, considered to be a primary risk factor in the development of hypertension. Additional mechanisms also related to endothelial dysfunction have been proposed to mediate the development of hypertension in obese individuals. These include: increase in both peripheral vasoconstriction and renal tubular sodium reabsorption, increased sympathetic activity and overactivation of both the renin-angiotensin system and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Obesity; Endothelial dysfunction; Oxidative stress; Renin-angiotensin system; Nitric oxide.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500003

Collateral methotrexate resistance in cisplatin-selected murine leukemia cells
Provedor de dados: 56
Autores: Bhushan,A.; Hacker,M.P.; Tritton,T.R.
Resistance to anticancer drugs is a major cause of failure of many therapeutic protocols. A variety of mechanisms have been proposed to explain this phenomenon. The exact mechanism depends upon the drug of interest as well as the tumor type treated. While studying a cell line selected for its resistance to cisplatin we noted that the cells expressed a &amp;gt;25,000-fold collateral resistance to methotrexate. Given the magnitude of this resistance we elected to investigate this intriguing collateral resistance. From a series of investigations we have identified an alteration in a membrane protein of the resistant cell as compared to the sensitive cells that could be the primary mechanism of resistance. Our studies reviewed here indicate decreased tyrosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methotrexate transport; Resistance; Cisplatin; Leukemia; Phosphorylation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700005

Clinical utility of standard base excess in the diagnosis and interpretation of metabolic acidosis in critically ill patients
Provedor de dados: 56
Autores: Park,M.; Taniguchi,L.U.; Noritomi,D.T.; Libório,A.B.; Maciel,A.T.; Cruz-Neto,L.M.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300016

Effect of sildenafil citrate on the cardiovascular system
Provedor de dados: 56
Autores: Shinlapawittayatorn,K.; Chattipakorn,S.; Chattipakorn,N.
Sildenafil citrate is a drug commonly used to manage erectile dysfunction. It is designated chemically as 1-[[3-(6,7-dihydro-1-methyl-7-oxo-3-propyl-1H -pyrazolo[4,3-d]pyrimidin-5-yl)-4 ethoxyphenyl] sulfonyl]-4-methylpiperazine citrate (C22H30N6O4 S). It is a highly selective inhibitor of cyclic guanine monophosphate-specific phosphodiesterase type 5. In late March through mid-November 1998, the US Food and Drug Administration (FDA) published a report on 130 confirmed deaths among men (mean age, 64 years) who received prescriptions for sildenafil citrate, a period during which &gt;6 million outpatient prescriptions (representing about 50 million tablets) were dispensed. The US FDA recently reported that significant cardiovascular events, including sudden...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sildenafil citrate; Erectile dysfunction; Sudden cardiac death; Cardiovascular disease; Phosphodiesterase inhibitor; Cardioprotection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900003

Clinical results of the use of mitotane for adrenocortical carcinoma
Provedor de dados: 56
Autores: Kasperlik-Zaluska,A.A.
Mitotane (o,p'-DDD) acts mainly as an inhibitor of intramitochondrial pregnenolone and cortisol synthesis. Its adrenolytic effect depends on metabolic activation due to conversion to o,p'-DDA and o,p'-DDE. The drug has been used for 40 years in the treatment of adrenocortical carcinoma, mainly its regional and metastatic stage, as an adjuvant to surgical resection of the tumor. In the medical literature there are controversial opinions about its efficacy for the treatment of adrenocortical carcinoma. In our experience, mitotane administered immediately after surgery appeared to be much more efficient than when administered later. We have administered this drug in all cases of microscopically confirmed adrenocortical carcinoma, irrespectively of stage at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitotane; Adrenocortical carcinoma; Cushing's syndrome.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000008

Effects of simultaneous exposure to stress and nicotine on nicotine-induced locomotor activation in adolescent and adult rats
Provedor de dados: 56
Autores: Zago,A.; Leão,R.M.; Carneiro-de-Oliveira,P.E.; Marin,M.T.; Cruz,F.C.; Planeta,C.S.
Preclinical studies have shown that repeated stress experiences can result in an increase in the locomotor response to the subsequent administration of drugs of abuse, a phenomenon that has been termed behavioral cross-sensitization. Behavioral sensitization reflects neuroadaptive processes associated with drug addiction and drug-induced psychosis. Although cross-sensitization between stress- and drug-induced locomotor activity has been clearly demonstrated in adult rats, few studies have evaluated this phenomenon in adolescent rats. In the present study, we determined if the simultaneous exposure to stress and nicotine was capable of inducing behavioral sensitization to nicotine in adolescent and adult rats. To this end, adolescent (postnatal day (P)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-sensitization; Locomotor activity; Nicotine; Stress; Adolescence.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100006

Strontium ranelate inhibits wear particle-induced aseptic loosening in mice
Provedor de dados: 56
Autores: Geng,Tianxiang; Sun,Shouxuan; Yu,Haochen; Guo,Haohui; Zheng,Mengxue; Zhang,Shuai; Chen,Xi; Jin,Qunhua.
The imbalance between bone formation and osteolysis plays a key role in the pathogenesis of aseptic loosening. Strontium ranelate (SR) can promote bone formation and inhibit osteolysis. The aim of this study was to explore the role and mechanism of SR in aseptic loosening induced by wear particles. Twenty wild-type (WT) female C57BL/6j mice and 20 sclerostin-/- female C57BL/6j mice were used in this study. Mice were randomly divided into four groups: WT control group, WT SR group, knockout (KO) control group, and KO SR group. We found that SR enhanced the secretion of osteocalcin (0.72±0.007 in WT control group, 0.98±0.010 in WT SR group, P=0.000), Runx2 (0.34±0.005 in WT control group, 0.47±0.010 in WT SR group, P=0.000), β-catenin (1.04±0.05 in WT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strontium ranelate; Aseptic loosening; Sclerostin; Bone remodeling; Canonical Wnt pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900605

Protective effect of maternal prenatal melatonin administration on rat pups born to mothers submitted to constant light during gestation
Provedor de dados: 56
Autores: Cisternas,C.D.; Compagnucci,M.V.; Conti,N.R.; Ponce,R.H.; Vermouth,N.T.
We studied the effects of adverse conditions such as constant light (LL) on the circadian rhythm of malate (MDH, EC 1.1.1.37) and lactate (LDH, EC 1.1.1.27) dehydrogenase activities of the testes of male Wistar rats on postnatal day 28 (PN28), anxiety-like behavior (elevated plus-maze test) at PN60 and sexual behavior at PN120. The rats were assigned to mother groups on day 10 of pregnancy: control (12-h light/dark), LL (light from day 10 to 21 of pregnancy), and LL+Mel (LL and sc injection to the mothers of a daily dose of melatonin, 1 mg/kg body weight at circadian time 12, from day 17 to 21 of pregnancy). LL offspring did not show circadian rhythms of MDH (N = 62) and LDH (N = 63) activities (cosinor and ANOVA-LSD Fisher). They presented a 44.7%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pineal hormone; Constant light; Entrainment; Copulatory behavior; Anxiety-like behavior.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900010

A 28-fold increase in secretory protein synthesis is associated with DNA puff activity in the salivary gland of Bradysia hygida (Diptera, Sciaridae)
Provedor de dados: 56
When the first group of DNA puffs is active in the salivary gland regions S1 and S3 of Bradysia hygida larvae, there is a large increase in the production and secretion of new salivary proteins demonstrable by [3H]-Leu incorporation. The present study shows that protein separation by SDS-PAGE and detection by fluorography demonstrated that these polypeptides range in molecular mass from about 23 to 100 kDa. Furthermore, these proteins were synthesized mainly in the S1 and S3 salivary gland regions where the DNA puffs C7, C5, C4 and B10 are conspicuous, while in the S2 region protein synthesis was very low. Others have shown that the extent of amplification for DNA sequences that code for mRNA in the DNA puffs C4 and B10 was about 22 and 10 times,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gene amplification; Secretory polypeptide synthesis; DNA puffs; Salivary glands of Sciaridae.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500006

Effect of race, genetic population structure, and genetic models in two-locus association studies: clustering of functional renin-angiotensin system gene variants in hypertension association studies
Provedor de dados: 56
Autores: Pereira,A.C.; Mota,G.A.; Benseñor,I.; Lotufo,P.A.; Krieger,J.E.
Previous genetic association studies have overlooked the potential for biased results when analyzing different population structures in ethnically diverse populations. The purpose of the present study was to quantify this bias in two-locus association studies conducted on an admixtured urban population. We studied the genetic structure distribution of angiotensin-converting enzyme insertion/deletion (ACE I/D) and angiotensinogen methionine/threonine (M/T) polymorphisms in 382 subjects from three subgroups in a highly admixtured urban population. Group I included 150 white subjects; group II, 142 mulatto subjects, and group III, 90 black subjects. We conducted sample size simulation studies using these data in different genetic models of gene action and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Association study; Genetics; Angiotensin-converting enzyme; ACE; Angiotensinogen.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100008

Interaction between human cytomegalovirus UL136 protein and ATP1B1 protein
Provedor de dados: 56
Autores: Cui,Xin; Sun,Zheng-Rong; Ren,Gao-Wei; Wang,Gui-Li; Qi,Ying; Ma,Yan-Ping; Ruan,Qiang.
Interplay between the host and human cytomegalovirus (HCMV) has a pivotal role in the outcome of infection. A region (referred to as UL/b’) present in the Toledo strain of HCMV and low passage clinical isolates contains 19 additional genes, which are absent in the highly passaged laboratory strain AD169. Products of the UL/b’ genes may determine the manifestations of HCMV infection in vivo. However, little is known about the host factors, which interact with UL/b’ proteins. This study was conducted to investigate the function of the HCMV UL136 protein. By yeast two-hybrid screening, the β1 subunit of the host Na+/K+-ATPase (ATP1B1) was identified to be a candidate protein, which interacts with the HCMV UL136 protein. The interaction was further evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCMV; UL136; ATPase; Yeast two hybridization.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200008

Effects of metformin treatment on luteal phase progesterone concentration in polycystic ovary syndrome
Provedor de dados: 56
Autores: Meenakumari,K.J.; Agarwal,S.; Krishna,A.; Pandey,L.K.
The causes of luteal phase progesterone deficiency in polycystic ovary syndrome (PCOS) are not known. To determine the possible involvement of hyperinsulinemia in luteal phase progesterone deficiency in women with PCOS, we examined the relationship between progesterone, luteinizing hormone (LH) and insulin during the luteal phase and studied the effect of metformin on luteal progesterone levels in PCOS. Patients with PCOS (19 women aged 18-35 years) were treated with metformin (500 mg three times daily) for 4 weeks prior to the test cycle and throughout the study period, and submitted to ovulation induction with clomiphene citrate. Blood samples were collected from control (N = 5, same age range as PCOS women) and PCOS women during the late follicular (one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycystic ovary syndrome; Metformin; Luteal phase defect; Progesterone.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100007

In vitro evaluation of the cytotoxic and trypanocidal activities of Ampelozizyphus amazonicus (Rhamnaceae)
Provedor de dados: 56
Autores: Rosas,L.V.; Cordeiro,M.S.C.; Campos,F.R.; Nascimento,S.K.R.; Januário,A.H.; França,S.C.; Nomizo,A.; Toldo,M.P.A.; Albuquerque,S.; Pereira,P.S.
Ampelozizyphus amazonicus Ducke is a tree commonly found in the Amazon region and an extract of its stem bark is popularly used as an antimalarial and anti-inflammatory agent and as an antidote to snake venom. Ursolic acid; five lupane type triterpenes: betulin, betulinic acid, lupenone, 3ß-hydroxylup-20(29)-ene-27,28-dioic acid, and 2a,3ß-dihydroxylup-20(29)-ene-27,28-dioic acid, and three phytosteroids: stigmasterol, sitosterol and campesterol, have been isolated from stem extracts of A. amazonicus Ducke. Their structures were characterized by spectral data including COSY and HMQC. In an in vitro biological screening of the isolated compounds, 3ß-hydroxylup-20(29)-ene-27,28-dioic acid was cytotoxic against the SKBR-3 human adenocarcinoma cell line (1 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ampelozizyphus amazonicus; Rhamnaceae; Pentacyclic triterpenes; Cytotoxic activity; Trypanocidal activity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500009

Cross-transmission of vancomycin-resistant Enterococcus in patients undergoing dialysis and kidney transplant
Provedor de dados: 56
Autores: Fram,D.; Castrucci,F.M.; Taminato,M.; Godoy-Martinez,P.; Freitas,M.C.S.; Belasco,A.; Sesso,R.; Pacheco-Silva,A.; Pignatari,A.C.; Barbosa,D.
The objective of this study was to investigate the occurrence of vancomycin-resistant Enterococcus (VRE) cross-transmission between two patient groups (long-term dialysis and kidney transplant patients). Molecular typing, by automated ribotyping with the RiboPrinter Microbial Characterization System (Qualicon, USA), was used to analyze VRE isolates from 31 fecal samples of 320 dialysis patients and 38 fecal samples of 280 kidney transplant patients. Clonal spread of E. faecalis and E. casseliflavus was observed intragroup, but not between the two groups of patients. In turn, transmission of E. gallinarum and E. faecium between the groups was suggested by the finding of vancomycin-resistant isolates belonging to the same ribogroup in both dialysis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vancomycin-resistant Enterococcus; Transmission; Dialysis; Kidney transplant.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100016

Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity
Provedor de dados: 56
Autores: Rodríguez,E.M.; Bigi,R.; Medesani,D.A.; Stella,V.S.; Greco,L.S.L.; Moreno,P.A.R.; Monserrat,J.M.; Pellerano,G.N.; Ansaldo,M.
Whole body oxygen consumption and some hemolymph parameters such as pH, partial pressure of gases, level of ions and lactate were measured in the estuarine crab Chasmagnathus granulata after both acute (96 h) and chronic (2 weeks) exposure to cadmium at concentrations ranging from 0.4 to 6.3 mg/l. In all instances, the crabs developed hemolymph acidosis, but no respiratory (increased PCO2) or lactate increases were evident. Hemolymph levels of sodium and calcium were always increased by cadmium exposure. The chronic toxicity of cadmium was enhanced at 12‰ salinity, even causing a significantly higher mortality in comparison with the higher salinity (30‰) used. A general metabolic arrest took place at 12‰ salinity in the crabs chronically exposed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cadmium; Crabs; Blood homeostasis; Salinity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400011

High specificity PCR screening for 22q11.2 microdeletion in three different ethnic groups
Provedor de dados: 56
Autores: Pereira,A.C.; Corrêa,R.F.R.; Mota,G.F.; Kim,C.A.; Mesquita,S.F.; Krieger,J.E.
Congenital heart defects are the most common of all human birth defects. Numerous studies have shown that a deletion within chromosome 22q11 is associated with DiGeorge syndrome and certain forms of sporadic congenital cardiovascular disease. We have determined the value of a PCR assay using markers D22S941, D22S944 and D22S264 designed for the screening of 22q11.2 deletion through consecutive homozygosity in an ethnically admixed urban population. The study population comprised 149 unrelated men and women from three different ethnic groups (white, mulatto and black). Test specificity for the overall population was estimated at 98.3%. We found no significant difference when comparing heterozygosity indices and ethnicity (P value = 0.43 (D22S944), 0.22...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DiGeorge syndrome; PCR screening; 22q11.2.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000012

Involvement of midbrain tectum neurokinin-mediated mechanisms in fear and anxiety
Provedor de dados: 56
Autores: Brenes,J.C.; Broiz,A.C.; Bassi,G.S.; Schwarting,R.K.W.; Brandão,M.L.
Electrical stimulation of midbrain tectum structures, particularly the dorsal periaqueductal gray (dPAG) and inferior colliculus (IC), produces defensive responses, such as freezing and escape behavior. Freezing also ensues after termination of dPAG stimulation (post-stimulation freezing). These defensive reaction responses are critically mediated by γ-aminobutyric acid and 5-hydroxytryptamine mechanisms in the midbrain tectum. Neurokinins (NKs) also play a role in the mediation of dPAG stimulation-evoked fear, but how NK receptors are involved in the global processing and expression of fear at the level of the midbrain tectum is yet unclear. The present study investigated the role of NK-1 receptors in unconditioned defensive behavior induced by electrical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurokinin-1 receptors; Dorsal periaqueductal gray; Spantide; Unconditioned fear; Elevated plus maze; Ultrasonic vocalizations.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400009

Extralymphatic disease due to bancroftian filariasis
Provedor de dados: 56
Autores: Dreyer,G.; Dreyer,P.; Piessens,W.F.
Infection with Wuchereria bancrofti, Brugia malayi, or B. timori not only affects the structure and function of lymphatic vessels but is also associated with extralymphatic pathology and disease. Because it is now possible to detect living adult worms by ultrasonography, much emphasis is placed on lymphatic pathology. However, the finding of renal damage in asymptomatic microfilaremic carriers has led to increased recognition of the importance of extralymphatic clinical manifestation in bancroftian filariasis. The authors present a number of clinical syndromes that may be manifestations of extralymphatic filarial disease and discuss possible mechanisms that cause these conditions. The main purpose of this paper is to raise the awareness of students and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wuchereria bancrofti; Microfilaria; Extralymphatic disease; Tropical pulmonary eosinophilia.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200003

Enhanced anti-tumor effect of a gene gun-delivered DNA vaccine encoding the human papillomavirus type 16 oncoproteins genetically fused to the herpes simplex virus glycoprotein D
Provedor de dados: 56
Autores: Diniz,M.O.; Ferreira,L.C.S.
Anti-cancer DNA vaccines have attracted growing interest as a simple and non-invasive method for both the treatment and prevention of tumors induced by human papillomaviruses. Nonetheless, the low immunogenicity of parenterally administered vaccines, particularly regarding the activation of cytotoxic CD8+ T cell responses, suggests that further improvements in both vaccine composition and administration routes are still required. In the present study, we report the immune responses and anti-tumor effects of a DNA vaccine (pgD-E7E6E5) expressing three proteins (E7, E6, and E5) of the human papillomavirus type 16 genetically fused to the glycoprotein D of the human herpes simplex virus type 1, which was administered to mice by the intradermal (id) route...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gene gun; DNA vaccine; HPV-16; Anti-cancer vaccine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500007

A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus
Provedor de dados: 56
Autores: Cardoso,T.C.; Mouro-Sousa,R.L.; Oliveira,C.; Stringhini,G.; Augusto-Pinto,A.
A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Liquid phase blocking ELISA; Humoral response; Infectious bronchitis virus.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600010

More than 10 years survival with sequential therapy in a patient with advanced renal cell carcinoma: a case report
Provedor de dados: 56
Autores: Yuan,J.L.; Wang,F.L.; Yi,X.M.; Qin,W.J.; Wu,G.J.; Huan,Y.; Yang,L.J.; Zhang,G.; Yu,L.; Zhang,Y.T.; Qin,R.L.; Tian,C.J.
Although radical nephrectomy alone is widely accepted as the standard of care in localized treatment for renal cell carcinoma (RCC), it is not sufficient for the treatment of metastatic RCC (mRCC), which invariably leads to an unfavorable outcome despite the use of multiple therapies. Currently, sequential targeted agents are recommended for the management of mRCC, but the optimal drug sequence is still debated. This case was a 57-year-old man with clear-cell mRCC who received multiple therapies following his first operation in 2003 and has survived for over 10 years with a satisfactory quality of life. The treatments given included several surgeries, immunotherapy, and sequentially administered sorafenib, sunitinib, and everolimus regimens. In the course...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Metastatic renal cell carcinoma; Sequential therapy; Targeted anticancer agents; Sorafenib; Sunitinib; Immunotherapy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100034

Indirect effects of oral tolerance to ovalbumin interfere with the immune responses triggered by Schistosoma mansoni eggs
Provedor de dados: 56
Autores: Carvalho,C.R.; Lenzi,H.L.; Correa-Oliveira,R.; Vaz,N.M.
The objective of the present study was to investigate whether the injection of a tolerated protein (indirect effects) affects the formation of granulomas around Schistosoma mansoni eggs trapped in the lungs after intravenous (iv) injection into normal (noninfected) C57BL/6 mice (6 animals per group). To induce oral tolerance to chicken egg ovalbumin a 1/5 dilution of egg white in water was offered ad libitum in a drinking bottle for 3 days. Control mice received water. After 7 days, control and experimental animals were injected iv with 2,000 S. mansoni eggs through a tail vein. In some mice of both groups the iv injection of eggs was immediately followed by intraperitoneal (ip) immunization with 10 µg of dinitrophenylated conjugates of ovalbumin (DNP-Ova)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Oral tolerance; Ovalbumin; Granuloma; Schistosoma mansoni.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000012

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
Provedor de dados: 56
Autores: Almeida,M.B.; Costa-Malaquias,A.; Nascimento,J.L.M.; Oliveira,K.R.; Herculano,A.M.; Crespo-López,M.E.
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphine; Glia; Oxidative stress; Hydrogen peroxide; Lipid peroxidation; Opiod.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500398

The calcium-modulated proteins, S100A1 and S100B, as potential regulators of the dynamics of type III intermediate filaments
Provedor de dados: 56
Autores: Garbuglia,M.; Verzini,M.; Sorci,G.; Bianchi,R.; Giambanco,I.; Agneletti,A.L.; Donato,R.
The Ca2+-modulated, dimeric proteins of the EF-hand (helix-loop-helix) type, S100A1 and S100B, that have been shown to inhibit microtubule (MT) protein assembly and to promote MT disassembly, interact with the type III intermediate filament (IF) subunits, desmin and glial fibrillary acidic protein (GFAP), with a stoichiometry of 2 mol of IF subunit/mol of S100A1 or S100B dimer and an affinity of 0.5-1.0 µM in the presence of a few micromolar concentrations of Ca2+. Binding of S100A1 and S100B results in inhibition of desmin and GFAP assemblies into IFs and stimulation of the disassembly of preformed desmin and GFAP IFs. S100A1 and S100B interact with a stretch of residues in the N-terminal (head) domain of desmin and GFAP, thereby blocking the head-to-tail...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S100A1; S100B; Intermediate filaments; Calcium; Regulatory effects; Assembly-disassembly.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000001

Changes in endogenous tissue glutathione level in relation to murine ascites tumor growth and the anticancer activity of cisplatin
Provedor de dados: 56
Autores: Khynriam,D.; Prasad,S.B.
Changes in glutathione levels were determined in tissues of 11- to 12-week-old Swiss albino mice at different stages of Dalton's lymphoma tumor growth and following cisplatin (8 mg/kg body weight, ip) treatment for 24-96 h, keeping 4-5 animals in each experimental group. Glutathione levels increased in spleen of tumor-bearing compared to normal mice (9.95 ± 0.14 vs 7.86 ± 1.64 µmol/g wet weight, P&lt;=0.05) but decreased in blood (0.64 ± 0.10 vs 0.85 ± 0.09 mg/ml) and testes (9.28 ± 0.15 vs 10.16 ± 0.28 µmol/g wet weight, P&lt;=0.05). Dalton's lymphoma cells showed an increase in glutathione concentration (4.43 ± 0.26 µmol/g wet weight) as compared to splenocytes, their normal counterpart (3.62 ± 0.41 µmol/g wet weight). With the progression of tumor in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Dalton's lymphoma; Glutathione; Glutathione-S-transferase; Chemotherapy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100008

Coronary fat content evaluated by morphometry in patients with severe atherosclerosis has no relation with serum lipid levels
Provedor de dados: 56
Autores: Braz Jr.,D.J.; Gutierrez,P.S.; Luz,P.L. da.
The relationship between lipid serum levels and coronary atherosclerotic plaque fat content was studied in 51 necropsy patients. Serum lipids were measured by standard techniques, during life, in the absence of lipid-lowering drugs. Intima, intimal fat and media areas were measured using a computerized system in cryosections of the odd segments of the right, anterior descending and circumflex coronary arteries stained with Sudan-IV. Mean intimal and lipid areas were 5.74 ± 1.98 and 1.22 ± 0.55 mm² (22.12 ± 8.48%) in 26 cases with high cholesterol (³200 mg/dL) and 4.98 ± 1.94 and 1.16 ± 0.66 mm² (22.75 ± 9.06%) in 25 cases with normal cholesterol (&lt;200 mg/dL; P &gt; 0.05). Patients with high levels of low-density lipoprotein (³130 mg/dL, N = 15) had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary arteriosclerosis; Cholesterol; Image cytometry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400004

Association of ApoE polymorphisms with prevalent hypertension in 1406 older adults: the Bambuí Health Aging Study (BHAS)
Provedor de dados: 56
Autores: Fuzikawa,A.K.; Peixoto,S.V.; Taufer,M.; Moriguchi,E.H.; Lima-Costa,M.F.
Tipo: Info:eu-repo/semantics/article
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700015

c-Fos expression induced by electroacupuncture at the Zusanli point in rats submitted to repeated immobilization
Provedor de dados: 56
Autores: Medeiros,M.A.; Canteras,N.S.; Suchecki,D.; Mello,L.E.A.M.
In laboratory animals, acupuncture needs to be performed on either anesthetized or, if unanesthetized, restrained subjects. Both procedures up-regulate c-Fos expression in several areas of the central nervous system, representing therefore a major pitfall for the assessment of c-Fos expression induced by electroacupuncture. Thus, in order to reduce the effect of acute restraint we used a protocol of repeated restraint for the assessment of the brain areas activated by electroacupuncture in adult male Wistar rats weighing 180-230 g. Repeated immobilization protocols (6 days, 1 h/day and 13 days, 2 h/day) were used to reduce the effect of acute immobilization stress on the c-Fos expression induced by electroacupuncture at the Zusanli point (EA36S). Animals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acupuncture; Zusanli point; Immediate early genes; C-Fos; Restraint; Habituation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200009

Montelukast reduces seizures in pentylenetetrazol-kindled mice
Provedor de dados: 56
Autores: Fleck,J.; Temp,F.R.; Marafiga,J.R.; Jesse,A.C.; Milanesi,L.H.; Rambo,L.M.; Mello,C.F.
Cysteinyl leukotrienes (CysLTs) have been implicated in seizures and kindling; however, the effect of CysLT receptor antagonists on seizure frequency in kindled animals and changes in CysLT receptor expression after pentylenetetrazol (PTZ)-induced kindling have not been investigated. In this study, we evaluated whether the CysLT1 inverse agonist montelukast, and a classical anticonvulsant, phenobarbital, were able to reduce seizures in PTZ-kindled mice and alter CysLT receptor expression. Montelukast (10 mg/kg, sc) and phenobarbital (20 mg/kg, sc) increased the latency to generalized seizures in kindled mice. Montelukast increased CysLT1immunoreactivity only in non-kindled, PTZ-challenged mice. Interestingly, PTZ challenge decreased CysLT2 immunoreactivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Montelukast; CysLT1R; CysLT2R; Seizure; PTZ; Kindling.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400602

Does whole body vibration exercise improve oxidative stress markers in women with fibromyalgia?
Provedor de dados: 56
Autores: Santos,J.M.; Mendonça,V.A.; Ribeiro,V.G.C.; Tossige-Gomes,R.; Fonseca,S.F.; Prates,A.C.N.; Flor,J.; Oliveira,A.C.C.; Martins,J.B.; Garcia,B.C.C.; Leite,H.R.; Figueiredo,P.H.S.; Bernardo-Filho,M.; Lacerda,A.C.R.
The objective of this study was to investigate the effect of whole body vibration (WBV) exercise on oxidative stress markers in a group of women with fibromyalgia (FM) compared to a group of healthy women (CT). Twenty-one women diagnosed with FM and 21 age- and weight-matched healthy women were enrolled the study. Plasma oxidative stress markers (primary outcomes) were evaluated at rest and after WBV, and included thiobarbituric acid reactive substances (TBARS), iron reduction capacity (FRAP), superoxide dismutase antioxidant enzymes activity (SOD), and catalase (CAT). At rest, the FM group had higher TBARS (P&lt;0.001) and FRAP (P&lt;0.001), and lower CAT (P=0.005) compared to the CT. In the CT group, the WBV had no effect on TBARS (P=0.559) and FRAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whole body vibration; Chronic disease; Oxidative stress; Fibromyalgia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800609

Antiproliferative effects of 1,25-dihydroxyvitamin D3 on breast cells: a mini review
Provedor de dados: 56
Autores: Bortman,P.; Folgueira,M.A.A.K.; Katayama,M.L.H.; Snitcovsky,I.M.L.; Brentani,M.M.
The hormone 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the active form of vitamin D3, is an important regulator of calcium homeostasis, exerts antiproliferative effects on various cell systems and can induce differentiation in some kinds of hematopoietic cells. These effects are triggered by its receptor, vitamin D receptor (VDR), a phosphoprotein member of the nuclear receptor superfamily, which functions as a transcriptional factor. VDR binds as a heterodimer with retinoid X receptor (R X R) to hexameric repeats, characterized as vitamin D-responsive elements present in the regulatory region of target genes such as osteocalcin, osteopontin, calbindin-D28K, calbindin-D9K, p21WAF1/CIP1, TGF-ß2 and vitamin D 24-hydroxylase. Many factors such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcitriol; Calcitriol receptor; Calcitriol analog; Breast tumor; Cell proliferation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100001

Factors associated with adherence to antiretroviral therapy in HIV/AIDS patients: a cross-sectional study in Southern Brazil
Provedor de dados: 56
Autores: Pinheiro,C.A.T.; de-Carvalho-Leite,J.C.; Drachler,M.L.; Silveira,V.L.
A cross-sectional study was conducted on HIV-infected adults being treated with antiretroviral drugs at a reference service in Southern Brazil. Participants answered a sociodemographic questionnaire and were tested by scales assessing sociocognitive variables. Adherence to treatment was assessed by a self-report inventory developed for the study. Clinical information was obtained from the patients' records. Significance tests were conducted using univariate logistic regressions followed by multivariate logistic regression analysis. A total of 195 patients participated in the study and 56.9% of them reported  &gt; or = 95% adherence on the previous two days. In univariate analysis, the odds of adherence increased with self-efficacy (a person's conviction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adherence; Antiretroviral therapy; Self-efficacy; HIV; AIDS.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000010

Effect of the Escherichia coli EMO strain on experimental infection by Salmonella enterica serovar Typhimurium in gnotobiotic mice
Provedor de dados: 56
Autores: Lima-Filho,J.V.M.; Vieira,L.Q.; Arantes,R.M.E.; Nicoli,J.R.
An experimental infection with Salmonella enterica subsp. enterica serovar Typhimurium was evaluated in gnotobiotic mice previously exposed to a plasmid-free non-pathogenic Escherichia coli (EMO strain). Mice were exposed to EMO (experimental) or not (control) 10 days before challenge with Salmonella Typhimurium (10² colony forming units (CFU)/mouse). Survival after challenge was higher (P &lt; 0.05) in the experimental group (16%) than in the control animals (0%). Histopathological examination of the colon and ileum mucosa of the experimental group showed less extensive lesions such as edema, cell inflammatory infiltration and hyperemia. The epithelial cells of the mucosal surface and the production of the mucous layer were also better preserved in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotic; Salmonella; Escherichia coli; Gnotobiotic mice.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700009

Zymosan phagocytosis by mouse peritoneal macrophages is increased by apoHDL- and not by intact HDL-covered particles
Provedor de dados: 56
Autores: Carvalho,M.D.T.; Tobias,V.E.; Vendrame,C.M.V.; Shimabukuro,A.F.M.; Gidlund,M.; Quintão,E.C.R.
The uptake of lipids and lipoprotein particles by macrophages undergoes phagocytic activation and the formation of foam cells are key events in atherosclerosis. In this study we determined how intact high density lipoproteins (HDL) and apolipoproteins-HDL (removal of the lipid component from HDL, i.e., apoHDL) influence the phagocytosis of zymosan by mouse peritoneal macrophages. Zymosan particles preincubated together with lipoproteins or alone (control) were incubated with the macrophages. Phagocytosis activity was reported as the percent of macrophages that internalized three or more zymosan particles. HDL co-incubated with zymosan did not influence the over-all uptake of zymosan particles compared to apoHDL, which greatly enhanced the ability of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HDL; ApoHDL; Zymosan particles; Atherosclerosis; Phagocytosis; Macrophages.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300009

Outward potassium current oscillations in macrophage polykaryons: extracellular calcium entry and calcium-induced calcium release
Provedor de dados: 56
Autores: Saraiva,R.M.; Masuda,M.O.; Oliveira-Castro,G.M.
Outward current oscillations associated with transient membrane hyperpolarizations were induced in murine macrophage polykaryons by membrane depolarization in the absence of external Na+. Oscillations corresponded to a cyclic activation of Ca2+-dependent K+ currents (IKCa) probably correlated with variations in intracellular Ca2+ concentration. Addition of external Na+ (8 mM) immediately abolished the outward current oscillations, suggesting that the absence of the cation is necessary not only for their induction but also for their maintenance. Oscillations were completely blocked by nisoldipine. Ruthenium red and ryanodine reduced the number of outward current cycles in each episode, whereas quercetin prolonged the hyperpolarization 2- to 15-fold. Neither...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrophage; Macrophage polykaryon; Ca2+-dependent K+ current; Membrane potential oscillations; Calcium-induced calcium release; Ca2+ ATPase.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100015

Morphological changes induced by testosterone in the mammary glands of female Wistar rats
Provedor de dados: 56
Autores: Chambô-Filho,A.; Camargos,A.F.; Pereira,F.E.L.
Increased levels of androgens in postmenopausal women are considered to be a risk factor for breast cancer. Testosterone, alone or in combination with estrogen, induces epithelial dysplasia and mammary tumors in Noble rats. Since this model of hormone-induced neoplasia has not been reported in other rat strains, we studied the effect of testosterone on the mammary gland morphology of female Wistar rats. Sixty adult, non-castrated, female Wistar rats were implanted in the dorsum midline with a silicone tube containing 50 mg testosterone (testosterone propionate in 30 animals and non-esterified testosterone in the remaining 30 animals) and 20 additional animals were implanted with empty tubes and used as control. Five animals per group were killed 30, 60,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Testosterone; Androgens; Mammary gland; Mammary gland hyperplasia; Mammary gland tumors.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400008

Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/β-catenin pathway
Provedor de dados: 56
Autores: Xu,Shuxiang; Fan,Yingying; Li,Dongping; Liu,Yan; Chen,Xu.
Cervical cancer is one of the most common cancers among women around the world. However, the underlying mechanism involved in cervical cancer progression is incompletely known. In the present study, we determined the role of glycoprotein nonmetastatic melanoma protein B (GPNMB) in tumorigenesis of cervical cancer. According to the GEO database, we found that GPNMB expression was significantly higher in cervical cancer than in normal cervix epithelium. A similar pattern was observed in GPNMB expression in cultured cervical cancer cells and normal cervical epithelial cells. Compared with the control, GPNMB knockdown significantly decreased the proliferation and migration capacity, but enhanced the apoptosis capacity of SiHa and HeLa cells. Additionally, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; GPNMB; Tumorigenesis; MMPs; Wnt/β-catenin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100607

Relationship between work rate and oxygen uptake in mitochondrial myopathy during ramp-incremental exercise
Provedor de dados: 56
Autores: Gimenes,A.C.; Neder,J.A.; Dal Corso,S.; Nogueira,C.R.; Nápolis,L.; Mello,M.T.; Bulle,A.S.; Nery,L.E.
We determined the response characteristics and functional correlates of the dynamic relationship between the rate (Δ) of oxygen consumption ( &lt;img border=0 width=11 height=13 src="548_files/image001.gif" v:shapes="_x0000_i1188"&gt;O2) and the applied power output (work rate = WR) during ramp-incremental exercise in patients with mitochondrial myopathy (MM). Fourteen patients (7 males, age 35.4 ± 10.8 years) with biopsy-proven MM and 10 sedentary controls (6 males, age 29.0 ± 7.8 years) took a ramp-incremental cycle ergometer test for the determination of the &lt;img border=0 width=11 height=13 src="548_files/image001.gif" v:shapes="_x0000_i1189"&gt;O2 on-exercise mean response time (MRT) and the gas exchange threshold (GET). The Δ&lt;img border=0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscular diseases; Oxygen consumption; Cardiopulmonary exercise testing; Skeletal muscle; Pathophysiology; Mitochondrial myopathy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400013

Protection against neurotoxicity by an autophagic mechanism
Provedor de dados: 56
Autores: Liu,Kangyong; Huang,Jiankang; Chen,Rongfu; Zhang,Ting; Shen,Liwei; Yang,Jiajun; Sun,Xiaojiang.
The objective of the present study was to investigate the effects of 3-n-butylphthalide (NBP) on a 1-methyl-4-phenylpyridinium (MPP+)-induced cellular model of Parkinson’s disease (PD) and to illustrate the potential mechanism of autophagy in this process. For this purpose, rat PC12 pheochromocytoma cells were treated with MPP+ (1 mM) for 24 h following pretreatment with NBP (0.1 mM). Cell metabolic viability was determined by the MTT assay and cell ultrastructure was examined by transmission electron microscopy. The intracellular distribution and expression of α-synuclein and microtubule-associated protein light chain 3 (LC3) were detected by immunocytochemistry and Western blotting. Our results demonstrated that: 1) NBP prevented MPP+-induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 3-N-butylphthalide; Parkinson’s disease; Α-synuclein; PC12 cells; Autophagy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500004

Variations in gastric compliance induced by acute blood volume changes in anesthetized rats
Provedor de dados: 56
Autores: Graça,J.R.V.; Leal,P.R.L.; Gondim,F.A.A.; Rola,F.H.; Santos,A.A.
The impact of acute volume imbalances on gastric volume (GV) was studied in anesthetized rats (250-300 g). After cervical and femoral vessel cannulation, a balloon catheter was positioned in the proximal stomach. The opposite end of the catheter was connected to a barostat with an electronic sensor coupled to a plethysmometer. A standard ionic solution was used to fill the balloon (about 3.0 ml) and the communicating vessel system, and to raise the reservoir liquid level 4 cm above the animals' xiphoid appendix. Due to constant barostat pressure, GV values were considered to represent the gastric compliance index. All animals were monitored for 90 min. After a basal interval, they were randomly assigned to normovolemic, hypervolemic, hypovolemic or...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Blood volume; Gastric compliance; Gastrointestinal motility; Hemorrhage; Hypervolemia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300018

The inflammatory stimulus of a natural latex biomembrane improves healing in mice
Provedor de dados: 56
Autores: Andrade,T.A.M.; Iyer,A.; Das,P.K.; Foss,N.T.; Garcia,S.B.; Coutinho-Netto,J.; Jordão-Jr.,A.A.; Frade,M.A.C.
The aim of the present study was to compare healing obtained with biomembranes with the natural healing process (sham) using biochemical and immunohistological assays. C57BL/6 mice were divided into 4 groups of 15 mice each and received different subcutaneous implants: natural latex biomembrane (NLB), denatured latex (DL), expanded polytetrafluorethylene (ePTFE), or sham. On the 2nd, 7th, and 14th days post-treatment, 5 mice per group were sacrificed and biopsied for the following measurements: oxidative stress based on malondialdehyde (MDA), myeloperoxidase (MPO) and hydrogen peroxide by the method of ferrous oxidation-xylenol orange (FOX), as well as glutathione and total proteins; histological evaluation to enumerate inflammatory cells, fibroblasts,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wound healing; Inflammation; Oxidative stress; Angiogenesis; Latex; Histomorphometry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000009

Effect of asthma severity on symptom perception in childhood asthma
Provedor de dados: 56
Autores: Cabral,A.L.B.; Conceição,G.M.; Saldiva,P.H.N.; Martins,M.A.
Individual ability to perceive airway obstruction varies substantially. The factors influencing the perception of asthma are probably numerous and not well established in children. The present study was designed to examine the influence of asthma severity, use of preventive medication, age and gender on the association between respiratory symptoms (RS) and peak expiratory flow (PEF) rates in asthmatic children. We followed 92 asthmatic children, aged 6 to 16 years, for five months. Symptom scores were recorded daily and PEF was measured twice a day. The correlations among variables at the within-person level over time were analyzed for each child and for the pooled data by multivariate analysis. After pooling the data, there was a significant (P&lt;0.05)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Asthma severity; Pulmonary function tests; Symptom perception; Childhood asthma.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300006

Adenovirus-mediated siRNA targeting TNF-α and overexpression of bone morphogenetic protein-2 promotes early osteoblast differentiation on a cell model of Ti particle-induced inflammatory response in vitro
Provedor de dados: 56
Autores: Guo,H.H.; Yu,C.C.; Sun,S.X.; Ma,X.J.; Yang,X.C.; Sun,K.N.; Jin,Q.H.
Wear particles are phagocytosed by macrophages and other inflammatory cells, resulting in cellular activation and release of proinflammatory factors, which cause periprosthetic osteolysis and subsequent aseptic loosening, the most common causes of total joint arthroplasty failure. During this pathological process, tumor necrosis factor-alpha (TNF-α) plays an important role in wear-particle-induced osteolysis. In this study, recombination adenovirus (Ad) vectors carrying both target genes [TNF-α small interfering RNA (TNF-α-siRNA) and bone morphogenetic protein 2 (BMP-2)] were synthesized and transfected into RAW264.7 macrophages and pro-osteoblastic MC3T3-E1 cells, respectively. The target gene BMP-2, expressed on pro-osteoblastic MC3T3-E1 cells and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF-α; BMP-2; Small interfering RNA; Titanium particles; Osteolysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000831

Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticity
Provedor de dados: 56
We have previously discovered a long-lasting enhancement of synaptic transmission in mammal autonomic ganglia caused by immunological activation of ganglionic mast cells. Subsequent to mast cell activation, lipid and peptide mediators are released which may modulate synaptic function. In this study we determined whether some mast cell-derived mediators, prostaglandin D2 (PGD2; 1.0 µM), platelet aggregating factor (PAF; 0.3 µM) and U44619 (a thromboxane analogue; 1.0 µM), and also endothelin-1 (ET-1; 0.5 µM) induce synaptic potentiation in the guinea pig superior cervical ganglion (SCG), and compared their effects on synaptic transmission with those induced by a sensitizing antigen, ovalbumin (OVA; 10 µg/ml). The experiments were carried out on SCGs...
Palavras-chave: Prostaglandin D; Platelet aggregating factor; Endothelin-1; Thromboxane; Mast cell; Synaptic plasticity.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700014

Glial cell activity is maintained during prolonged inflammatory challenge in rats
Provedor de dados: 56
Autores: Borges,B.C.; Rorato,R.; Antunes-Rodrigues,J; Elias,L.L.K.
We evaluated the expression of glial fibrillary acidic protein (GFAP), glutamine synthetase (GS), ionized calcium binding adaptor protein-1 (Iba-1), and ferritin in rats after single or repeated lipopolysaccharide (LPS) treatment, which is known to induce endotoxin tolerance and glial activation. Male Wistar rats (200-250 g) received ip injections of LPS (100 µg/kg) or saline for 6 days: 6 saline (N = 5), 5 saline + 1 LPS (N = 6) and 6 LPS (N = 6). After the sixth injection, the rats were perfused and the brains were collected for immunohistochemistry. After a single LPS dose, the number of GFAP-positive cells increased in the hypothalamic arcuate nucleus (ARC; 1 LPS: 35.6 ± 1.4 vs control: 23.1 ± 2.5) and hippocampus (1 LPS: 165.0 ± 3.0 vs control: 137.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LPS tolerance; Astrocytes; Microglia; Glial fibrillary acidic protein; Iba-1; Glutamine synthetase; Ferritin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800014

Antinociceptive effects of the essential oil of Croton nepetaefolius on mice
Provedor de dados: 56
Autores: Abdon,A.P.V.; Leal-Cardoso,J.H.; Coelho-de-Souza,A.N.; Morais,S.M.; Santos,C.F.
Croton nepetaefolius Baill., is an aromatic plant native to the northeast of Brazil where it is extensively used in folk medicine as a sedative, orexigen and antispasmodic agent. In the present study the antinociceptive effects of the essential oil of C. nepetaefolius (EOCn), administered orally, were evaluated in male Swiss mice (20-25 g). In the acetic acid-induced writhing test, EOCn (100 and 300 mg/kg; N = 14 and N = 12, respectively) was effective at the highest dose. In the hot-plate test, EOCn at 30 and 300 mg/kg, but not at 3 mg/kg, significantly increased the latency at all observation times up to the 180th min (N = 12 for each dose). In the formalin test, EOCn significantly reduced paw licking in the second phase of the test at 100 mg/kg (N =...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Croton nepetaefolius; Essential oil; Analgesic; Antinociception; Formalin test; Hot-plate test.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000015

Effect of gender on training-induced vascular remodeling in SHR
Provedor de dados: 56
Autores: Amaral,S.L.; Michelini,L.C.
There is accumulating evidence that physical inactivity, associated with the modern sedentary lifestyle, is a major determinant of hypertension. It represents the most important modifiable risk factor for cardiovascular diseases, which are the leading cause of morbidity and mortality for both men and women. In addition to involving sympathetic overactivity that alters hemodynamic parameters, hypertension is accompanied by several abnormalities in the skeletal muscle circulation including vessel rarefaction and increased arteriole wall-to-lumen ratio, which contribute to increased total peripheral resistance. Low-intensity aerobic training is a promising tool for the prevention, treatment and control of high blood pressure, but its efficacy may differ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; Hypertension; Microcirculation; Arterioles; Capillaries; Venules.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900001

The alga Bryothamnion seaforthii contains carbohydrates with antinociceptive activity
Provedor de dados: 56
Autores: Vieira,L.A.P.; Freitas,A.L.P.; Feitosa,J.P.A.; Silva,D.C.; Viana,G.S.B.
Bryothamnion seaforthii, a red alga common to the Northeastern coast of Brazil, was used to prepare the protein fraction F0/60 by ammonium sulfate precipitation. The chromatography of F0/60 on DEAE-Sephadel column resulted in two lectin fractions, PI and PII, which have antinociceptive properties in rodents. We determined the antinociceptive activity of the PII fraction and of a carbohydrate-containing fraction (CF) in mice. The CF was prepared from the dried algae, after digestion with 100 mM sodium acetate, pH 6.0, containing 5 mM cysteine, EDTA and 0.4% papain, at 60ºC. A 10% cetylpyridinium chloride was added to the filtrate, and the precipitate was dissolved with 2 M NaCl:ethanol (100:15, v/v) followed by the carbohydrate precipitation with ethanol....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Algae; Antinociceptive activity; Carbohydrate; Bryothamnion seaforthii.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700017

Effect of metabolic control on interferon-gamma and interleukin-10 production by peripheral blood mononuclear cells from type 1 and type 2 diabetic patients
Provedor de dados: 56
Autores: Foss-Freitas,M.C.; Foss,N.T.; Donadi,E.A.; Foss,M.C.
The objective of the present study was to evaluate the production of cytokines, interferon-g (INF-g) and interleukin-10 (IL-10), in cultures of peripheral blood mononuclear cells (PBMC) from type 1 and type 2 diabetic patients and to correlate it with inadequate and adequate metabolic control. We studied 11 type 1 and 13 type 2 diabetic patients and 21 healthy individuals divided into two groups (N = 11 and 10) paired by sex and age with type 1 and type 2 diabetic patients. The PBMC cultures were stimulated with concanavalin-A to measure INF-g and IL-10 supernatant concentration by ELISA. For patients with inadequate metabolic control, the cultures were performed on the first day of hospitalization and again after intensive treatment to achieve adequate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 1 diabetes mellitus; Type 2 diabetes mellitus; Cytokines; Interferon-gamma; Interleukin-10; Metabolic control.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500010

An HPLC-UV method for the measurement of permeability of marker drugs in the Caco-2 cell assay
Provedor de dados: 56
Autores: Kratz,J.M.; Teixeira,M.R.; Koester,L.S.; Simões,C.M.O.
The Caco-2 cell line has been used as a model to predict the in vitro permeability of the human intestinal barrier. The predictive potential of the assay relies on an appropriate in-house validation of the method. The objective of the present study was to develop a single HPLC-UV method for the identification and quantitation of marker drugs and to determine the suitability of the Caco-2 cell permeability assay. A simple chromatographic method was developed for the simultaneous determination of both passively (propranolol, carbamazepine, acyclovir, and hydrochlorothiazide) and actively transported drugs (vinblastine and verapamil). Separation was achieved on a C18 column with step-gradient elution (acetonitrile and aqueous solution of ammonium acetate, pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caco-2; HPLC-UV; Marker drugs; Permeability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600006

LncRNA SNHG12 promotes cell growth and inhibits cell apoptosis in colorectal cancer cells
Provedor de dados: 56
Autores: Wang,J.Z.; Xu,C.L.; Wu,H.; Shen,S.J.
Several long non-coding RNA (lncRNA) might be correlated with the prognosis of colorectal cancer (CRC) and serve as a diagnostic and prognostic biomarker. However, the exact expression pattern of small nucleolar RNA host gene 12 (SNHG12) in colorectal cancer and its clinical significance remains unclear. The level of SNHG12 was detected by qRT-PCR in CRC tissues and CRC cells. MTT assay and colony formation assay were performed to examine the cell proliferation of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12. Flow cytometry technology was used to detect cell cycle and cell apoptosis of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12. The protein level of cell cycle progression-related molecules, including cyclin-dependent kinases (CDK4, CDK6),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SNHG12; Cell growth; Biomarker; Cell apoptosis; Colorectal cancer.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300604

Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury
Provedor de dados: 56
Autores: Nicola,F.C.; Rodrigues,L.P.; Crestani,T.; Quintiliano,K.; Sanches,E.F.; Willborn,S.; Aristimunha,D.; Boisserand,L.; Pranke,P.; Netto,C.A.
Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Stem cells; Treadmill training; Functional recovery; MASCIS impactor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900604

Antioxidant effect of 4-nerolidylcatechol and α-tocopherol in erythrocyte ghost membranes and phospholipid bilayers
Provedor de dados: 56
Autores: Fernandes,K.S.; Silva,A.H.M.; Mendanha,S.A.; Rezende,K.R.; Alonso,A.
4-Nerolidylcatechol (4-NC) is found in Pothomorphe umbellataroot extracts and is reported to have a topical protective effect against UVB radiation-induced skin damage, toxicity in melanoma cell lines, and antimalarial activity. We report a comparative study of the antioxidant activity of 4-NC and α-tocopherol against lipid peroxidation initiated by two free radical-generating systems: 2,2′-azobis(2-aminopropane) hydrochloride (AAPH) and FeSO4/H2O2, in red blood cell ghost membranes and in egg phosphatidylcholine (PC) vesicles. Lipid peroxidation was monitored by membrane fluidity changes assessed by electron paramagnetic resonance spectroscopy of a spin-labeled lipid and by the formation of thiobarbituric acid-reactive substances. When lipoperoxidation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electron paramagnetic resonance 4-Nerolidylcatechol Spin label Lipid peroxidation DPPH (2; 2-diphenyl-1-picrylhydrazyl).
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900780

Hypoglycemic activity of polysaccharide fractions containing ß-glucans from extracts of Rhynchelytrum repens (Willd.) C.E. Hubb., Poaceae
Provedor de dados: 56
Autores: De Paula,A.C.C.F.F.; Sousa,R.V.; Figueiredo-Ribeiro,R.C.L.; Buckeridge,M.S.
ß-Glucans are soluble fibers with physiological functions, such as interference with absorption of sugars and reduction of serum lipid levels. The objective of the present study was to analyze the distribution of ß-glucans in different tissues of the African grass species Rhynchelytrum repens and also to evaluate their hypoglycemic activity. Leaf blades, sheaths, stems, and young leaves of R. repens were submitted to extraction with 4 M KOH. Analysis of the fractions revealed the presence of arabinose, glucose, xylose, and traces of rhamnose and galactose. The presence of ß-glucan in these fractions was confirmed by hydrolyzing the polymers with endo-ß-glucanase from Bacillus subtilis, followed by HPLC analysis of the characteristic oligosaccharides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SS-Glucans; Cell wall; Hypoglycemic activity; Diabetes mellitus; Grasses; Rhynchelytrum repens.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600010

Prognostic significance of snail expression in hilar cholangiocarcinoma
Provedor de dados: 56
Autores: Kong,Dalu; Liang,Jun; Li,Rong; Liu,Shihai; Wang,Jigang; Zhang,Kejun; Chen,Dong.
Many patients with hilar cholangiocarcinoma (HC) have a poor prognosis. Snail, a transcription factor and E-cadherin repressor, is a novel prognostic factor in many cancers. The aim of this study was to evaluate the relationship between snail and E-cadherin protein expression and the prognostic significance of snail expression in HC. We examined the protein expression of snail and E-cadherin in HC tissues from 47 patients (22 males and 25 females, mean age 61.2 years) using immunohistochemistry and RT-PCR. Proliferation rate was also evaluated in the same cases by the MIB1 index. High, low and negative snail protein expression was recorded in 18 (38%), 17 (36%), and 12 (26%) cases, respectively, and 40.4% (19/47) cases showed reduced E-cadherin protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hilar cholangiocarcinoma; Prognostic marker; Snail; E-cadherin; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700008

Baicalin reduces the severity of experimental autoimmune encephalomyelitis
Provedor de dados: 56
Autores: Zeng,Y.; Song,C.; Ding,X.; Ji,X.; Yi,L.; Zhu,K.
Scutellaria baicalensis Georgi is one of the important medicinal herbs widely used for the treatment of various inflammatory diseases in Asia. Baicalin (BA) is a bioactive anti-inflammatory flavone found abundantly in Scutellaria baicalensis Georgi. To explore the therapeutic potential of BA, we examined the effects of systemic administration of the flavone (5 and 10 mg/kg, ip) on relapsing/remitting experimental autoimmune encephalomyelitis (EAE) induced by proteolipid protein 139-151 in SJL/J mice, an experimental model of multiple sclerosis. The mice treated with PBS or BA at day -1 and for 3 consecutive days were observed daily for clinical signs of disease up to 60 days after immunization. In the PBS-EAE group, neurological scores were: incidence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental autoimmune encephalomyelitis; Baicalin; IFN-g; IL-4; Neurologic score.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700016

Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain
Provedor de dados: 56
Autores: Schilichting,C.L.R.; Lima,K.C.M.; Cestari Junior,L.A.; Sekiyama,J.Y.; Silva,F.M.; Milani,H.
A gravimetric method was evaluated as a simple, sensitive, reproducible, low-cost alternative to quantify the extent of brain infarct after occlusion of the medial cerebral artery in rats. In ether-anesthetized rats, the left medial cerebral artery was occluded for 1, 1.5 or 2 h by inserting a 4-0 nylon monofilament suture into the internal carotid artery. Twenty-four hours later, the brains were processed for histochemical triphenyltetrazolium chloride (TTC) staining and quantitation of the schemic infarct. In each TTC-stained brain section, the ischemic tissue was dissected with a scalpel and fixed in 10% formalin at 0ºC until its total mass could be estimated. The mass (mg) of the ischemic tissue was weighed on an analytical balance and compared to its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Focal cerebral ischemia; Infarct size; Rats; Triphenyltetrazolium chloride stain; Weighing method.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400008

Co-transfection of hepatocyte growth factor and truncated TGF-β type II receptor inhibit scar formation
Provedor de dados: 56
Autores: Xu,Ji-Hua; Zhao,Wan-Yi; Fang,Qing-Qing; Wang,Xiao-Feng; Zhang,Ding-Ding; Hu,Yan-Yan; Zheng,Bin; Tan,Wei-Qiang.
Wound scarring remains a major challenge for plastic surgeons. Transforming growth factor (TGF)-β plays a key role in the process of scar formation. Previous studies have demonstrated that truncated TGF-β type II receptor (t-TGF-βRII) is unable to continue signal transduction but is still capable of binding to TGF-β, thereby blocking the TGF-β signaling pathway. Hepatocyte growth factor (HGF) is a multifunctional growth factor that promotes tissue regeneration and wound healing. Theoretically, the combination of HGF and t-TGF-βRII would be expected to exert a synergistic effect on promoting wound healing and reducing collagen formation. In the present study, lentivirus-mediated transfection of the two genes (t-TGF-βRII/HGF) into fibroblasts in vitro and in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wound healing; Truncated transforming growth factor-β type II receptor; Hepatocyte growth factor; Scar; Rat; Lentivirus.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100611

Immunomodulatory properties of Alternanthera tenella Colla aqueous extracts in mice
Provedor de dados: 56
Autores: Guerra,R.N.M.; Pereira,H.-A.W.; Silveira,L.M.S.; Olea,R.S.G.
Plants from the genus Alternanthera are thought to possess antimicrobial and antiviral properties. In Brazilian folk medicine, the aqueous extract of A. tenella Colla is used for its anti-inflammatory activity. The present study investigated the immunomodulatory property of A. tenella extract by evaluating the antibody production in male albino Swiss mice weighing 20-25 g (10 per group). The animals received standard laboratory diet and water ad libitum. The effect of A. tenella extract (5 and 50 mg/kg, ip) was evaluated in mice immunized with sheep red blood cells (SRBC 10%, ip) as T-dependent antigen, or in mice stimulated with mitogens (10 µg, Escherichia coli lipopolysaccharide, LPS, ip). The same doses (5 and 50 mg/kg, ip) of A. tenella extract were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antitumor activity; Antibody; Immunomodulation; Ehrlich ascites; Alternanthera.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900011

Urethroplasty using a bovine pericardium graft: an experimental study using normal urethras from dogs
Provedor de dados: 56
Autores: Lara,R.C.; Lucon,A.M.; Arap,S.
The use of bovine pericardium as a urethral patch to substitute a ventral segment of canine urethras was studied. Healing, epithelial growth, urethral permeability, fistulas, and calcification were analyzed. Thirty male mongrel dogs of medium and large size underwent resection of a ventral segment of the medial urethra measuring 2.0 x 0.5 cm, which was replaced with a bovine pericardium graft, treated with buffered glutaraldehyde and preserved in formaldehyde. Two running sutures of polygalactin 5-0 were applied, one on each side of the patch. The corpus spongiosum was closed with uninterrupted suture and the skin with interrupted suture of polygalactin 5-0. Six months later, the animals were examined and sacrificed under anesthesia. Retrograde...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urethra; Urethral stricture; Bovine pericardium.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300006

Circulating myeloid-derived suppressor cells predict disease activity and treatment response in patients with immune thrombocytopenia
Provedor de dados: 56
Autores: Zhou,J.; Zhou,Y.; Wen,J.; Sun,X.; Zhang,X.
Immune thrombocytopenia (ITP) is a disease characterized by isolated thrombocytopenia. Abnormal effector T cell activation is an important mechanism in the pathogenesis of ITP. Regulatory T cells (Treg) have a strong immunosuppressive function for T cell activation and their importance in the pathophysiology and clinical treatment of ITP has been confirmed. Myeloid-derived suppressor cells (MDSCs) are other immunosuppressive cells, which can also suppress T cell activation by secreting arginase, iNOS and ROS, and are essential for Treg cells’ differentiation and maturation. Therefore, we speculate that MDSCs might also be involved in the immune-dysregulation mechanism of ITP. In this study, we tested MDSCs and Treg cells in peripheral blood samples of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myeloid-derived suppressor cells; Immune thrombocytopenia; Regulatory T cells; Disease activity; Treatment response.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200704

Effect of chronic oral administration of a low dose of captopril on sodium appetite of hypothyroid rats: Influence of aldosterone treatment
Provedor de dados: 56
Autores: Ventura,R.R.; Olivares,E.L.; Passos Junior,D.B.; Ramalho,M.J.; Antunes-Rodrigues,J.; Reis,L.C.
Rats rendered hypothyroid by treatment with methimazole develop an exaggerated sodium appetite. We investigated here the capacity of hypothyroid rats (N = 12 for each group) to respond to a low dose of captopril added to the ration, a paradigm which induces an increase in angiotensin II synthesis in cerebral areas that regulate sodium appetite by increasing the availability of circulating angiotensin I. In addition, we determined the influence of aldosterone in hypothyroid rats during the expression of spontaneous sodium appetite and after captopril treatment. Captopril significantly increased (P&lt;0.05) the daily intake of 1.8% NaCl (in ml/100 g body weight) in hypothyroid rats after 36 days of methimazole administration (day 36: 9.2 ± 0.7 vs day 32: 2.8...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sodium appetite; Hypothyroidism; Captopril; Aldosterone; Rats.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300016

The effect of 677C&gt;T and 1298A&gt;C MTHFR polymorphisms on sulfasalazine treatment outcome in rheumatoid arthritis
Provedor de dados: 56
Autores: Pawlik,A.; Kurzawski,M.; Gawronska-Szklarz,B.; Gornik,W.; Dziedziejko,V.; Safranow,K.; Juzyszyn,Z.; Drozdzik,M.
Despite the availability of several new agents for the treatment of rheumatoid arthritis (RA), sulfasalazine remains the mainstay because of both cost and experience with its use. Methylenetetrahydrofolate reductase (MTHFR) is involved in folate metabolism and several polymorphisms have been described in the MTHFR gene. Of these, the 677C&gt;T and 1298A&gt;C polymorphisms have been associated with altered enzyme activity. To examine the association between 677C&gt;T and 1298A&gt;C MTHFR polymorphisms and sulfasalazine efficacy for the treatment of RA, a total of 117 RA patients treated with sulfasalazine (1 g daily; duration of treatment 17 ± 5 months) were analyzed. The 677C&gt;T and 1298 A&gt;C polymorphisms were detected using a PCR-RFLP method. RA was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MTHFR; Polymorphisms; Sulfasalazine; Rheumatoid arthritis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700011

Retraction notice for: Therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis. [Braz J Med Biol Res (2018) 51(2): e6812]
Provedor de dados: 56
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600950

Measurement of epidermal thickness in a patient with psoriasis by computer-supported image analysis
Provedor de dados: 56
Autores: Alper,M.; Kavak,A.; Parlak,A.H.; Demirci,R.; Belenli,I.; Yesildal,N.
The aim of the present study was to measure full epidermal thickness, stratum corneum thickness, rete length, dermal papilla widening and suprapapillary epidermal thickness in psoriasis patients using a light microscope and computer-supported image analysis. The data obtained were analyzed in terms of patient age, type of psoriasis, total body surface area involvement, scalp and nail involvement, duration of psoriasis, and family history of the disease. The study was conducted on 64 patients and 57 controls whose skin biopsies were examined by light microscopy. The acquired microscopic images were transferred to a computer and measurements were made using image analysis. The skin biopsies, taken from different body areas, were examined for different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psoriasis; Epidermis; Thickness; Computer-supported image analysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100015

A randomized double-blind study of the short-time treatment of obese patients with nonalcoholic fatty liver disease with ursodeoxycholic acid
Provedor de dados: 56
Autores: Santos,V.N.; Lanzoni,V.P.; Szejnfeld,J.; Shigueoka,D.; Parise,E.R.
In order to determine the effect of ursodeoxycholic acid on nonalcoholic fatty liver disease, 30 patients with body mass indices higher than 25, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) or gamma-glutamyltransferase (gamma-GT) at least more than 1.5 times the upper limit of normality, and hepatic steatosis demonstrated by ultrasonography were randomized into two groups of 15 patients to receive placebo or 10 mg kg-1 day-1 ursodeoxycholic acid for three months. Abdominal computed tomography was performed to quantify hepatic fat content, which was significantly correlated with histological grading of steatosis (r s = -0.83, P &lt; 0.01). Patient body mass index remained stable for both groups throughout the study, but a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nonalcoholic fatty liver disease; Nonalcoholic steatohepatitis; Fatty liver; Ursodeoxycholic acid.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600007

Partially folded intermediates during trypsinogen denaturation
Provedor de dados: 56
Autores: Martins,N.F.; Santoro,M.M.
The equilibrium unfolding of bovine trypsinogen was studied by circular dichroism, differential spectra and size exclusion HPLC. The change in free energy of denaturation was &lt;img SRC="http:/img/fbpe/bjmbr/v32n6/3282sup.gif" ALIGN="BOTTOM" BORDER="0" VSPACE="0" HSPACE="0"&gt; = 6.99 ± 1.40 kcal/mol for guanidine hydrochloride and &lt;img SRC="http:/img/fbpe/bjmbr/v32n6/3282sup.gif" ALIGN="BOTTOM" BORDER="0" VSPACE="0" HSPACE="0"&gt; = 6.37 ± 0.57 kcal/mol for urea. Satisfactory fits of equilibrium unfolding transitions required a three-state model involving an intermediate in addition to the native and unfolded forms. Size exclusion HPLC allowed the detection of an intermediate population of trypsinogen whose Stokes radii varied from 24.1 ± 0.4 Å to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypsinogen; Protein denaturation; Thermodynamic stability; Intermediates; Molten globule.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600002

Participation of endogenous opioids in the antinociception induced by resistance exercise in rats
Provedor de dados: 56
Autores: Galdino,G.S.; Duarte,I.D.G.; Perez,A.C.
Exercise is a low-cost intervention that promotes health and contributes to the maintenance of the quality of life. The present study was designed to investigate the influence of different resistance exercise protocols on the nociceptive threshold of rats. Female Wistar rats were used to perform exercises in a weight-lifting exercise model. The following groups were examined (N = 6 per group): untrained rats (control group); an acute protocol group consisting of rats submitted to 15 sets of 15 repetitions of resistance exercise (acute group); rats exercised with 3 sets of 10 repetitions, three times per week for 12 weeks (trained group), and a group consisting of trained rats that were further submitted to the acute protocol (trained-acute group). The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Resistance exercise; Opioid receptors; Antinociception.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900014

Multigenerational Brazilian family with malignant hyperthermia and a novel mutation in the RYR1 gene
Provedor de dados: 56
Autores: Matos,A.R.; Sambuughin,N.; Rumjanek,F.D.; Amoedo,N.D.; Cunha,L.B.P.; Zapata-Sudo,G.; Sudo,R.T.
Malignant hyperthermia (MH) is a pharmacogenetic disease triggered in susceptible individuals by the administration of volatile halogenated anesthetics and/or succinylcholine, leading to the development of a hypermetabolic crisis, which is caused by abnormal release of Ca2+ from the sarcoplasmic reticulum, through the Ca2+ release channel ryanodine receptor 1 (RyR1). Mutations in the RYR1 gene are associated with MH in the majority of susceptible families. Genetic screening of a 5-generation Brazilian family with a history of MH-related deaths and a previous MH diagnosis by the caffeine halothane contracture test (CHCT) in some individuals was performed using restriction and sequencing analysis. A novel missense mutation, Gly4935Ser, was found in an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant hyperthermia; Mutation; Ryanodine receptor; Calcium channel.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200016

Enhanced external counterpulsation improves cardiac function in Beagles after cardiopulmonary resuscitation
Provedor de dados: 56
Autores: Xiong,Jing; Zhang,Wei; Wei,Hongyan; Li,Xin; Dai,Gang; Hu,Chunlin.
The aim of this study was to investigate the influence of enhanced external counterpulsation (EECP) on the cardiac function of beagle dogs after prolonged ventricular fibrillation. Twenty-four adult male beagles were randomly divided into control and EECP groups. Ventricular fibrillation was induced in the animals for 12 min, followed by 2 min of cardiopulmonary resuscitation. They then received EECP therapy for 4 h (EECP group) or not (control group). The hemodynamics was monitored using the PiCCO2 system. Blood gas and hemorheology were assessed at baseline and at 1, 2, and 4 h after return of spontaneous circulation (ROSC). The myocardial blood flow (MBF) was quantified by 18F-flurpiridaz PET myocardial perfusion imaging at baseline and 4 h after ROSC....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiopulmonary resuscitation; Hemodynamics; Enhanced external counterpulsation; PiCCO2; Myocardial blood flow; Beagle dog.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100609

Post-exercise changes in blood pressure, heart rate and rate pressure product at different exercise intensities in normotensive humans
Provedor de dados: 56
Autores: Forjaz,C.L.M.; Matsudaira,Y.; Rodrigues,F.B.; Nunes,N.; Negrão,C.E.
To evaluate the effect of exercise intensity on post-exercise cardiovascular responses, 12 young normotensive subjects performed in a randomized order three cycle ergometer exercise bouts of 45 min at 30, 50 and 80% of VO2peak, and 12 subjects rested for 45 min in a non-exercise control trial. Blood pressure (BP) and heart rate (HR) were measured for 20 min prior to exercise (baseline) and at intervals of 5 to 30 (R5-30), 35 to 60 (R35-60) and 65 to 90 (R65-90) min after exercise. Systolic, mean, and diastolic BP after exercise were significantly lower than baseline, and there was no difference between the three exercise intensities. After exercise at 30% of VO2peak, HR was significantly decreased at R35-60 and R65-90. In contrast, after exercise at 50 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise intensity; Blood pressure; Heart rate; Rate pressure product.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000003

Longitudinal evaluation of hepatic osteodystrophy in children and adolescents with chronic cholestatic liver disease
Provedor de dados: 56
Autores: Taveira,A.T.A.; Pereira,F.A.; Fernandes,M.I.M.; Sawamura,R.; Nogueira-Barbosa,M.H.; Paula,F.J.A.
Bone mass loss is a major complication of chronic cholestatic liver disease (CCD). However, the long-term impact of CCD on bone mass acquisition is unknown. We longitudinally assessed bone mineral density (BMD) and factors involved in bone remodeling in 9 children and adolescents with CCD Child-Pugh A (5 boys/4 girls) and in 13 controls (6 boys/7 girls). The groups were evaluated twice, at baseline (T0) and after 3 years (T1), when osteocalcin, deoxypyridinoline, 25-hydroxyvitamin-D, parathyroid hormone, insulin-like growth factor-I (IGF-I), and BMD (L1-L4, proximal femur and total body) were determined. Serum levels of receptor activator for nuclear factor kB ligand (RANKL) and osteoprotegerin were measured only at T1. Lumbar spine BMD was reanalyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic osteodystrophy; Insulin-like growth factor-I; Osteoporosis; Bone mineral density; Bone remodeling.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100017

Influence of antihypertensive drugs on aortic and coronary effects of Ang-(1-7) in pressure-overloaded rats
Provedor de dados: 56
Autores: Nunes,A.D.C.; Souza,A.P.S.; Macedo,L.M.; Alves,P.H.; Pedrino,G.R.; Colugnati,D.B.; Mendes,E.P.; Santos,R.A.S.; Castro,C.H.
This study investigated the influence of antihypertensive drugs, such as angiotensin-converting enzyme inhibitors (ACEIs), AT1 receptor blockers (ARBs), voltage-gated L-type calcium channel blockers, and mineralocorticoid receptor antagonists (MRAs), on the effects of angiotensin-(1-7) [Ang-(1-7)] on aorta and coronary arteries from pressure-overloaded rats. Pressure overload was induced by abdominal aortic banding (AB). To evaluate the role of antihypertensive drugs on the effect of Ang-(1-7), AB male Wistar rats weighing 250–300 g were treated with vehicle or low doses (5 mg·kg-1·day-1, gavage) of losartan, captopril, amlodipine, or spironolactone. Isolated aortic rings and isolated perfused hearts under constant flow were used to evaluate the effect of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin-(1-7); Renin-angiotensin system; Coronary artery; Antihypertensive drugs; Cardiac hypertrophy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400604

Effect of hypoxia on the activity and binding of glycolytic and associated enzymes in sea scorpion tissues
Provedor de dados: 56
Autores: Lushchak,V.I.; Bahnjukova,T.V.; Storey,K.B.
The effect of hypoxia on the levels of glycogen, glucose and lactate as well as the activities and binding of glycolytic and associated enzymes to subcellular structures was studied in brain, liver and white muscle of the teleost fish, Scorpaena porcus. Hypoxia exposure decreased glucose levels in liver from 2.53 to 1.70 µmol/g wet weight and in muscle led to its increase from 3.64 to 25.1 µmol/g wet weight. Maximal activities of several enzymes in brain were increased by hypoxia: hexokinase by 23%, phosphoglucoisomerase by 47% and phosphofructokinase (PFK) by 56%. However, activities of other enzymes in brain as well as enzymes in liver and white muscle were largely unchanged or decreased during experimental hypoxia. Glycolytic enzymes in all three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Sea scorpion; Scorpaena porcus; Hypoxia; Metabolites; Glycolytic enzyme binding.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800005

Low vaspin levels are related to endothelial dysfunction in patients with ankylosing spondylitis
Provedor de dados: 56
Autores: Wang,H.H.; Wang,Q.F.
Vaspin is a novel adipocytokine associated with glucose tolerance and chronic inflammation. Some studies reveal that vaspin may be involved in cardiovascular diseases. Our objective was to investigate the relationship between serum vaspin levels and endothelial function in patients with ankylosing spondylitis. One hundred and twenty patients with newly diagnosed ankylosing spondylitis and 100 healthy subjects were studied. Serum vaspin levels were measured with enzyme-linked immunosorbent assay. High resolution ultrasound was used to measure brachial artery diameter at rest, after reactive hyperemia (flow-mediated dilation, FMD) and after sublingual glyceryltrinitrate. Serum vaspin level in patients was 1.92±1.03 ng/mL, which was significantly lower than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaspin; Endothelial function; Ankylosing spondylitis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700705

Effect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals
Provedor de dados: 56
Autores: Luna,T.; Santos,S.B.; Nascimento,M.; Porto,M.A.F.; Muniz,A.L.; Carvalho,E.M.; Jesus,A.R.
Human T lymphotropic virus type 1 (HTLV-1) is the causal agent of myelopathy/tropical spastic paraparesis (HAM/TSP), a disease mediated by the immune response. HTLV-1 induces a spontaneous proliferation and production of pro-inflammatory cytokines by T cells, and increasing interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) levels are potentially involved in tissue damage in diseases related to HTLV-1. This exaggerated immune response is also due to an inability of the natural regulatory mechanisms to down-modulate the immune response in this group of patients. TNF-α inhibitors reduce inflammation and have been shown to improve chronic inflammatory diseases in clinical trials. The aim of this study was to evaluate the ability of pentoxifylline,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTLV-1; TNF-α inhibitors; Pentoxifylline; Immune response.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100009

Effects of stress on catecholamine stores in central and peripheral tissues of long-term socially isolated rats
Provedor de dados: 56
Autores: Dronjak,S.; Gavrilovic,L.
Both the peripheral sympatho-adrenomedullary and central catecholaminergic systems are activated by various psycho-social and physical stressors. Catecholamine stores in the hypothalamus, hippocampus, adrenal glands, and heart auricles of long-term socially isolated (21 days) and control 3-month-old male Wistar rats, as well as their response to immobilization of all 4 limbs and head fixed for 2 h and cold stress (4ºC, 2 h), were studied. A simultaneous single isotope radioenzymatic assay based on the conversion of catecholamines to the corresponding O-methylated derivatives by catechol-O-methyl-transferase in the presence of S-adenosyl-l-(³H-methyl)-methionine was used. The O-methylated derivatives were oxidized to ³H-vanilline and the radioactivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cold stress; Immobilization stress; Catecholamines; Hypothalamus; Hippocampus; Adrenal glands.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600011

Intima-media thickness evaluation by B-mode ultrasound: Correlation with blood pressure levels and cardiac structures
Provedor de dados: 56
Autores: Plavnik,F.L.; Ajzen,S.; Kohlmann Jr.,O.; Tavares,A.; Zanella,M.T.; Ribeiro,A.B.; Ramos,O.L.
The aim of this study was to analyze the thickness of the intima-media complex (IMC) using a noninvasive method. The carotid and femoral common arteries were evaluated by noninvasive B-mode ultrasound in 63 normotensive and in 52 hypertensive subjects and the thickness of the IMC was tested for correlation with blood pressure, cardiac structures and several clinical and biological parameters. The IMC was thicker in hypertensive than in normotensive subjects (0.67 ± 0.13 and 0.62 ± 0.16 vs 0.54 ± 0.09 and 0.52 ± 0.11 mm, respectively, P&amp;lt;0.0001). In normotensive patients, the simple linear regression showed significant correlations between IMC and age, body mass index and 24-h systolic blood pressure for both the carotid and femoral arteries. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arterial thickness; B-mode ultrasound; 24-ABPM; Cardiac structures.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100008

Chronic lactose intake modifies the gastric emptying of monosaccharides but not of disaccharides in weanling rats
Provedor de dados: 56
Ninety-six weanling male Wistar rats were fed for four weeks one of two different chows: a normal rat chow containing 55.5% (w/w) starch (control group, N = 48) or a rat chow in which starch was partially replaced by lactose, in such a way that the experimental group (N = 48) received 35.5% (w/w) starch and 20% (w/w) lactose. The gastric emptying of fluid was then studied by measuring the gastric retention of four test meals containing lactose (5% or 10%, w/v) or glucose + galactose (5% or 10%, w/v). Homogenates of the small intestine were assayed for lactase activity. The gastric retention values were obtained 15 min after orogastric infusion of the liquid meals. The median values for gastric retention of the 5% lactose solutions were 37.7% for the...
Palavras-chave: Gastric emptying; Intestinal motility; Lactase; Lactose.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600004

Influence of body mass and environmental oxygen tension on the oxygen consumption rates of an enteropneust, Glossobalanus crozieri
Provedor de dados: 56
Autores: Ditadi,A.S.F.; Mendes,E.G.; Bianconcini,M.S.C.
The present study deals with a species of enteropneust, Glossobalanus crozieri, focusing on two aspects of its respiration: a) oxygen consumption and body mass, and b) the influence of environmental oxygen tension on the respiratory rate. Preliminarily, the body water content was shown to be 85% of the whole body weight. The regression coefficient of the oxygen consumption on the wet body mass (0.578) seems to agree with the view that in enteropneusts respiration is mainly cutaneous. The respiratory rate was significantly reduced at O2 tensions from 76 mmHg downwards, suggesting conformity rather than regulation
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enteropneusta; Glossobalanus crozieri; Respiration; Body mass; Oxygen tension.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200010

Highlights of the 3rd International Conference on High Pressure Bioscience and Biotechnology
Provedor de dados: 56
Autores: Mignaco,J.A.; Lima,L.M.T.R.; Rosenthal,A.; Foguel,D.; Silva,J.L.
The 3rd International Conference on High Pressure Bioscience and Biotechnology was held in the city of Rio de Janeiro from September 27 to September 30, 2004. The meeting, promoted by the International Association of High Pressure Bioscience and Biotechnology (IAHPBB), congregated top scientists and researchers from all over the world. In common, they shared the use of hydrostatic pressure for research, technical development, or industrial applications. The meeting consisted of invited lectures, contributed papers and a well-attended poster session. Very exciting discussions were held inside and outside the sessions, and the goals of discussing state-of-the-art data and establishing working collaborations and co-operations were fully attained.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrostatic pressure; Phase diagram; Compressibility; Protein folding; Food technology; Piezotolerance.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800001

Glutathione and the redox control system trypanothione/trypanothione reductase are involved in the protection of Leishmania spp. against nitrosothiol-induced cytotoxicity
Provedor de dados: 56
Autores: Romão,P.R.T.; Tovar,J.; Fonseca,S.G.; Moraes,R.H.; Cruz,A.K.; Hothersall,J.S.; Noronha-Dutra,A.A.; Ferreira,S.H.; Cunha,F.Q.
Glutathione is the major intracellular antioxidant thiol protecting mammalian cells against oxidative stress induced by oxygen- and nitrogen-derived reactive species. In trypanosomes and leishmanias, trypanothione plays a central role in parasite protection against mammalian host defence systems by recycling trypanothione disulphide by the enzyme trypanothione reductase. Although Kinetoplastida parasites lack glutathione reductase, they maintain significant levels of glutathione. The aim of this study was to use Leishmania donovani trypanothione reductase gene mutant clones and different Leishmania species to examine the role of these two individual thiol systems in the protection mechanism against S-nitroso-N-acetyl-D,L-penicillamine (SNAP), a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Glutathione; Trypanothione; Trypanothione reductase; Nitric oxide; Free radicals.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300006

Modulation of extracellular matrix by nutritional hepatotrophic factors in thioacetamide-induced liver cirrhosis in the rat
Provedor de dados: 56
Autores: Guerra,R.R.; Trotta,M.R.; Parra,O.M.; Avanzo,J.L.; Bateman,A.; Aloia,T.P.A.; Dagli,M.L.Z.; Hernandez-Blazquez,F.J.
Nutritional substances associated to some hormones enhance liver regeneration when injected intraperitoneally, being denominated hepatotrophic factors (HF). Here we verified if a solution of HF (glucose, vitamins, salts, amino acids, glucagon, insulin, and triiodothyronine) can revert liver cirrhosis and how some extracellular matrices are affected. Cirrhosis was induced for 14 weeks in 45 female Wistar rats (200 mg) by intraperitoneal injections of thioacetamide (200 mg/kg). Twenty-five rats received intraperitoneal HF twice a day for 10 days (40 mL·kg-1·day-1) and 20 rats received physiological saline. Fifteen rats were used as control. The HF applied to cirrhotic rats significantly: a) reduced the relative mRNA expression of the genes: Col-α1 (-53%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver cirrhosis; Hepatotrophic factors; Collagen; Matrix modulation; Progranulin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100005

Mesenchymal stem cells from patients with chronic myeloid leukemia do not express BCR-ABL and have absence of chimerism after allogeneic bone marrow transplant
Provedor de dados: 56
Autores: Carrara,R.C.V.; Orellana,M.D.; Fontes,A.M.; Palma,P.V.B.; Kashima,S.; Mendes,M.R.; Coutinho,M.A.; Voltarelli,J.C.; Covas,D.T.
Bone marrow is a heterogeneous cell population which includes hematopoietic and mesenchymal progenitor cells. Dysregulated hematopoiesis occurs in chronic myelogenous leukemia (CML), being caused at least in part by abnormalities in the hematopoietic progenitors. However, the role of mesenchymal stem cells (MSCs) in CML has not been well characterized. The objectives of the present study were to observe the biological characteristics of MSCs from CML patients and to determine if MSCs originate in part from donors in CML patients after bone marrow transplantation (BMT). We analyzed MSCs from 5 untreated patients and from 3 CML patients after sex-mismatched allogeneic BMT. Flow cytometry analysis revealed the typical MSC phenotype and in vitro assays showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Chimerism; Allogeneic hematopoietic stem cell transplantation; Chronic myeloid leukemia; Bcr-abl gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100008

Arginine induces GH gene expression by activating NOS/NO signaling in rat isolated hemi-pituitaries
Provedor de dados: 56
Autores: Olinto,S.C.F.; Adrião,M.G.; Castro-Barbosa,T.; Goulart-Silva,F.; Nunes,M.T.
The amino acid arginine (Arg) is a recognized secretagogue of growth hormone (GH), and has been shown to induce GH gene expression. Arg is the natural precursor of nitric oxide (NO), which is known to mediate many of the effects of Arg, such as GH secretion. Arg was also shown to increase calcium influx in pituitary cells, which might contribute to its effects on GH secretion. Although the mechanisms involved in the effects of Arg on GH secretion are well established, little is known about them regarding the control of GH gene expression. We investigated whether the NO pathway and/or calcium are involved in the effects of Arg on GH gene expression in rat isolated pituitaries. To this end, pituitaries from approximately 170 male Wistar rats (~250 g) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arginine; GH mRNA expression; Nitric oxide; Calcium (Ca2+); Cyclic guanosine monophosphate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100011

Repair of the anophthalmic cavity of rats with synthetic hydroxyapatite
Provedor de dados: 56
Autores: Ranzani,J.J.; Rahal,S.C.; Schellini,S.A.; Marques,M.E.A.; Taga,E.M.
We placed spheres of synthetic hydroxyapatite (calcium chloride combined with sodium phosphate) in the eviscerated or enucleated orbital cavity of rats in order to evaluate the biocompatibility of this material with the orbital cavity. The study was conducted on 50 albino rats, 25 of which were submitted to enucleation and 25 to evisceration of one eye. The animals were sacrificed 7, 15, 21, 30 and 60 days after surgery and the orbital content was submitted to histopathological examination. A reaction of the young granulation tissue type was observed first. The hydroxyapatite was gradually surrounded by a granulomatous macrophage inflammatory response and covered with dense connective tissue that formed a sort of" mesh" septating and supporting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synthetic hydroxyapatite; Reconstruction of the anophthalmic cavity; Experimental rats.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000007

Hippocampal overexpression of Down syndrome cell adhesion molecule in amyloid precursor protein transgenic mice
Provedor de dados: 56
Autores: Jia,Y.L.; Fu,Z.X.; Zhang,B.H.; Jia,Y.J.
Down syndrome cell adhesion molecule (DSCAM) is located within the Down syndrome critical region of chromosome 21. DSCAM is a broadly expressed neurodevelopmental protein involved in synaptogenesis, neurite outgrowth, and axon guidance. We previously demonstrated DSCAM overexpression in the cortex of amyloid precursor protein (APP) transgenic mice, suggesting possible regulatory interactions between APP and DSCAM. APP mice exhibit deficits in hippocampus-dependent learning and memory. In this preliminary study, we examined age-related changes in DSCAM expression within the hippocampus in 16 APP transgenic mice (1, 3, 6 and 12 months old). Hippocampus-dependent spatial memory was assessed in APP mice and age-matched wild type littermates (WTs) using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Down syndrome cell adhesion molecule; Amyloid precursor protein; Transgenic mouse; Hippocampus; Dementia.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600603

Metronomic chemotherapy in the neoadjuvant setting: results of two parallel feasibility trials (TraQme and TAME) in patients with HER2+ and HER2− locally advanced breast cancer
Provedor de dados: 56
Autores: Petry,V.; Gagliato,D.M.; Leal,A.I.C.; Arai,R.J.; Longo,E.; Andrade,F.; Ricci,M.D.; Piato,J.R.; Barroso-Sousa,R.; Hoff,P.M.; Mano,M.S.
Neoadjuvant chemotherapy has practical and theoretical advantages over adjuvant chemotherapy strategy in breast cancer (BC) management. Moreover, metronomic delivery has a more favorable toxicity profile. The present study examined the feasibility of neoadjuvant metronomic chemotherapy in two cohorts [HER2+ (TraQme) and HER2− (TAME)] of locally advanced BC. Twenty patients were prospectively enrolled (TraQme, n=9; TAME, n=11). Both cohorts received weekly paclitaxel at 100 mg/m2 during 8 weeks followed by weekly doxorubicin at 24 mg/m2 for 9 weeks in combination with oral cyclophosphamide at 100 mg/day (fixed dose). The HER2+ cohort received weekly trastuzumab. The study was interrupted because of safety issues. Thirty-six percent of patients in the TAME...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Metronomic chemotherapy; HER2+.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500479

Ischemic preconditioning vs adenosine vs prostaglandin E1 for protection against liver ischemia/reperfusion injury
Provedor de dados: 56
Autores: Radojkovic,M.; Stojanovic,M.; Stanojevic,G.; Radojkovic,D.; Gligorijevic,J.; Ilic,I.; Stojanovic,N.
Ischemia/reperfusion injury is still a major cause of morbidity and mortality during liver surgery and transplantation. A variety of surgical and pharmacological therapeutic strategies have been investigated to minimize the effects of ischemia/reperfusion. The aim of our study was to analyze and compare preventive influences of ischemic preconditioning, adenosine and prostaglandin E1 in the experimental model of hepatic ischemia/reperfusion injury. Adult chinchilla rabbits were divided into four groups: 10 rabbits subjected to liver ischemic preconditioning (3-min period of inflow occlusion followed by a 5-min period of reperfusion) followed by 45 min of Pringle maneuver; 10 rabbits subjected to pre-treatment with intraportal injection of adenosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver ischemia/reperfusion injury; Ischemic preconditioning; Adenosine; Prostaglandin E1; Protection.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800611

Efficacy of the dietary histone deacetylase inhibitor butyrate alone or in combination with vitamin A against proliferation of MCF-7 human breast cancer cells
Provedor de dados: 56
Autores: Andrade,F.O.; Nagamine,M.K.; Conti,A. De; Chaible,L.M.; Fontelles,C.C.; Jordão Junior,A.A.; Vannucchi,H.; Dagli,M.L.Z.; Bassoli,B.K.; Moreno,F.S.; Ong,T.P.
The combined treatment with histone deacetylase inhibitors (HDACi) and retinoids has been suggested as a potential epigenetic strategy for the control of cancer. In the present study, we investigated the effects of treatment with butyrate, a dietary HDACi, combined with vitamin A on MCF-7 human breast cancer cells. Cell proliferation was evaluated by the crystal violet staining method. MCF-7 cells were plated at 5 x 10(4) cells/mL and treated with butyrate (1 mM) alone or combined with vitamin A (10 µM) for 24 to 120 h. Cell proliferation inhibition was 34, 10 and 46% following treatment with butyrate, vitamin A and their combination, respectively, suggesting that vitamin A potentiated the inhibitory activities of butyrate. Furthermore, exposure to this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; MCF-7 cells; Butyric acid; Vitamin A; Epigenetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900008

Revealing potential long non-coding RNA biomarkers in lung adenocarcinoma using long non-coding RNA-mediated competitive endogenous RNA network
Provedor de dados: 56
Autores: Zhu,T-G.; Xiao,X.; Wei,Q.; Yue,M.; Zhang,L-X.
In our study, we aimed to reveal potential long non-coding RNAs (lncRNA) biomarkers in lung adenocarcinoma (LAD) using lncRNA-mediated competing endogenous RNAs (ceRNAs) network (LMCN). Competing lncRNA-mRNA interactions were identified using the hypergeometric test. Co-expression analysis for the competing lncRNA-mRNA interactions was implemented, and relying on the weight value &gt;0.8, a highly competitive LMCN was further constructed. Degree distribution, betweenness and closeness for LMCN were carried out to analyze the network structure. Functional analyses of mRNAs in LMCN were carried out to further explore the biological functions of lncRNAs. Biclique algorithm was utilized to extract competing modules from the LMCN. Finally, we verified our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung adenocarcinoma; Competing endogenous RNA; Long non-coding RNA; Hub; Diagnosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900606

A safety and feasibility study of cell therapy in dilated cardiomyopathy
Provedor de dados: 56
Autores: Martino,H.F.; Oliveira,P.S.; Souza,F.C.; Costa,P.C.; Assunção e Silva,E.; Villela,R.; Gaze,M.; Weitzel,L.H.; Oliveira Jr.,A.; Muccillo,F.B.; Arvelo,S.N.S.; Sá,R.; Guimarães,T.C.F.; Tura,B.R.; Campos de Carvalho,A.C.
The aim of this study was to determine if bone marrow mononuclear cell (BMMC) transplantation is safe for moderate to severe idiopathic dilated cardiomyopathy (IDC). Clinical trials have shown that this procedure is safe and effective for ischemic patients, but little information is available regarding non-ischemic patients. Twenty-four patients with IDC, optimized therapy, age 46 ± 11.6 years, 17 males, NYHA classes II-IV, and left ventricular ejection fraction &lt;35% were enrolled in the study. Clinical evaluation at baseline and 6 months after stem cell therapy to assess heart function included echocardiogram, magnetic resonance imaging, cardiopulmonary test, Minnesota Quality of Life Questionnaire, and NYHA classification. After cell transplantation 1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow cells; Mononuclear fraction; Autologous transplantation; Ejection fraction; Treadmill test; Dilated cardiomyopathy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000011

Simultaneous detection of the C282Y, H63D and S65C mutations in the hemochromatosis gene using quenched-FRET real-time PCR
Provedor de dados: 56
Autores: Moysés,C.B.; Moreira,E.S.; Asprino,P.F.; Guimarães,G.S.; Alberto,F.L.
Hereditary hemochromatosis (HH) is a common autosomal disorder of iron metabolism mainly affecting Caucasian populations. Three recurrent disease-associated mutations have been detected in the hemochromatosis gene (HFE): C282Y, H63D, and S65C. Although HH phenotype has been associated with all three mutations, C282Y is considered the most relevant mutation responsible for hemochromatosis. Clinical complications of HH include cirrhosis of the liver, congestive cardiac failure and cardiac arrhythmias, endocrine pancreatic disease, which can be prevented by early diagnosis and treatment. Therefore, a reliable genotyping method is required for presymptomatic diagnosis. We describe the simultaneous detection of the C282Y, H63D and S65C mutations in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hemochromatosis; Single nucleotide polymorphism; Quenched-FRET; Real-time PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000001

The rate of force generation by the myocardium is not influenced by afterload
Provedor de dados: 56
Autores: Fioretto,J.R.; Okoshi,M.P.; Okoshi,K.; Cicogna,A.C.; Bonatto,R.C.; Padovani,C.R.; Tucci,P.J.F.
The influence of afterload on the rate of force generation by the myocardium was investigated using two types of preparations: the in situ dog heart (dP/dt) and isolated papillary muscle of rats (dT/dt). Thirteen anesthetized, mechanically ventilated and thoracotomized dogs were submitted to pharmacological autonomic blockade (3.0 mg/kg oxprenolol plus 0.5 mg/kg atropine). A reservoir connected to the left atrium permitted the control of left ventricular end-diastolic pressure (LVEDP). A mechanical constriction of the descending thoracic aorta allowed to increase the systolic pressure in two steps of 20 mmHg (conditions H1 and H2) above control values (condition C). After arterial pressure elevations (systolic pressure C: 119 ± 8.1; H1: 142 ± 7.9; H2 166 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ventricular function; Afterload; DP/dt; DT/dt; Papillary muscle; Isometric contractions.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200015

Differences in functional and structural properties of segments of the rat tail artery
Provedor de dados: 56
Autores: Souza,F.M.; Padilha,A.S.; Stefanon,I.; Vassallo,D.V.
The investigation of resistance vessels is generally costly and difficult to execute. The present study investigated the diameters and the vascular reactivity of different segments of the rat tail artery (base, middle, and tail end) of 30 male Wister rats (EPM strain) to characterize a conductance or resistance vessel, using a low-cost simple technique. The diameters (mean ± SEM) of the base and middle segments were 471 ± 4.97 and 540 ± 8.39 µm, respectively, the tail end was 253 ± 2.58 µm. To test reactivity, the whole tail arteries or segments were perfused under constant flow and the reactivity to phenylephrine (PHE; 0.01-300 µg) was evaluated before and after removal of the endothelium or drug administration. The maximal response (Emax) and sensitivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat tail artery; Phenylephrine; Resistance artery; Endothelium; Conductance artery.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500011

Food intake and weight of lactating rats maintained on different protein-calorie diets, and pup growth
Provedor de dados: 56
Autores: Cambraia,R.P.B.; Vannucchi,H.; De-Oliveira,L.M.
Studies on rats maintained on low-protein-calorie diets during the lactation period show that food intake decreases. This process results in weight loss and a delay in litter development. The purpose of the present study was to determine the alterations in food intake, maternal weight and litter growth during lactation when dams were exposed to diets with different levels of protein and carbohydrate. Female Wistar rats receiving one of 4 different diets, A (N = 14), B (N = 14), C (N = 9) and D (N = 9), were used. Diet A contained 16% protein and 66% carbohydrate; diet B, 6% protein and 77% carbohydrate; diet C, 6% protein and 66% carbohydrate; diet D, 16% protein and 56% carbohydrate. Thus, C and D diets were hypocaloric, while A and B were isocaloric. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactation; Malnutrition; Low-protein-calorie diet; Feeding behavior; Weight and growth of rats.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800012

Why are the rates of cesarean section in Brazil higher in more developed cities than in less developed ones?
Provedor de dados: 56
Autores: Ribeiro,V.S.; Figueiredo,F.P.; Silva,A.A.M.; Bettiol,H.; Batista,R.F.L.; Coimbra,L.C.; Lamy,Z.C.; Barbieri,M.A.
The objective of the present study was to investigate factors associated with cesarean sections in two cities located in different regions of Brazil and to determine factors that explain the higher cesarean section rate in the more developed city, Ribeirão Preto, compared to the less developed one, São Luís. Data from two cohort studies comprising 2846 women in Ribeirão Preto in 1994, and 2443 women in São Luís in 1997/1998 were used. Adjusted and non-adjusted risk estimates were calculated using a Poisson regression model. The cesarean section rate was 33.7% in São Luís and 50.8% in Ribeirão Preto. Adjusted analysis in a joint sequential model revealed a 51% higher risk of cesarean section in Ribeirão Preto compared to São Luís (prevalence rate ratio...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cesarean section; Epidemiology; Obstetrics; Health services; Risk factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900008

Effects of elevated calcium on motor and exploratory activities of rats
Provedor de dados: 56
Autores: Godinho,A.F.; Trombini,T.V.; Oliveira,E.C.
The effects of serum and brain calcium concentration on rat behavior were tested by maintaining animals on either distilled water (N = 60) or water containing 1% calcium gluconate (N = 60) for 3 days. Animals that were maintained on high calcium drinking water presented increased serum calcium levels (control = 10.12 ± 0.46 vs calcium treated = 11.62 ± 0.51 µg/dl). Increase of brain calcium levels was not statistically significant. In the behavioral experiments each rat was used for only one test. Rats that were maintained on high calcium drinking water showed increased open-field behavior of ambulation (20.68%) and rearing (64.57%). On the hole-board, calcium-supplemented animals showed increased head-dip (67%) and head-dipping (126%), suggesting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium supplementation; Behavior; Rat; Motor activity; Exploratory activity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400007

Diurnal variation of vascular diameter and reactivity in healthy young men
Provedor de dados: 56
Autores: Bau,P.F.D.; Bau,C.H.D.; Naujorks,A.A.; Rosito,G.A.; Fuchs,F.D.
The higher incidence of cardiovascular events in the morning is accompanied by an increased vascular tone. However, there are few published studies designed to evaluate the diurnal variation of vascular and endothelial parameters in healthy subjects. In the present investigation, we evaluated the diurnal variation in brachial artery diameter (BAD), flow-mediated dilation (FMD) and endothelium-independent dilation (NFMD) in a homogeneous sample of healthy non-smoker young men. Fifty subjects aged 20.8 ± 0.3 years (range: 18 to 25 years) were investigated by brachial artery ultrasound. Exclusion criteria were female gender and evidence of clinically significant health problems, including obesity. Volunteers were asked to rest and avoid fat meals as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular risk factor; Circadian rhythm; Brachial artery; Vasoconstriction; Vascular biology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600011

Effect of 4-aminoantipyrine on gastric compliance and liquid emptying in rats
Provedor de dados: 56
Autores: Vinagre,A.M.; Collares,E.F.
Dipyrone (Dp) delays gastric emptying (GE) in rats. There is no information about whether 4-aminoantipyrine (AA), one of its metabolites, has the same effect. The objectives of the present study were to assess the effects of AA and Dp on GE when administered intravenously (iv) and intracerebroventricularly (icv) (240 µmol/kg and 4 µmol/animal, respectively) and on gastric compliance when administered iv (240 µmol/kg). GE was determined in male Wistar rats weighing 250-300 g (5-10 per group) after icv or iv injection of the drug by measuring percent gastric retention (GR) of a saline meal labeled with phenol red 10 min after administration by gavage. Gastric compliance was estimated in anesthetized rats (10-11 per group), with the construction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Gastric compliance; 4-Aminoantipyrine; Dipyrone; Vagus nerve.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700003

Cytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR
Provedor de dados: 56
Autores: Aquino,V.H.; Figueiredo,L.T.M.
A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection in blood and urine samples during the post-transplantation period. CMV was detected more frequently in blood samples than in urine samples (P&lt;0.001). Thirty-three patients had CMV detected at least once in blood and/or urine samples. Seven of these patients (21.2%) were diagnosed as having symptomatic CMV infection and showed a worse clinical outcome, with a higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus; Polymerase chain reaction; Kidney transplantation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100011

The role of osteoblasts in regulating hematopoietic stem cell activity and tumor metastasis
Provedor de dados: 56
Autores: Neiva,K.; Sun,Y.-X.; Taichman,R.S.
Bone marrow stromal cells are critical regulators of hematopoiesis. Osteoblasts are part of the stromal cell support system in bone marrow and may be derived from a common precursor. Several studies suggested that osteoblasts regulate hematopoiesis, yet the entire mechanism is not understood. It is clear, however, that both hematopoietic precursors and osteoblasts interact for the production of osteoclasts and the activation of resorption. We observed that hematopoietic stem cells (HSCs) regulate osteoblastic secretion of various growth factors, and that osteoblasts express some soluble factors exclusively in the presence of HSCs. Osteoblasts and hematopoietic cells are closely associated with each other in the bone marrow, suggesting a reciprocal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoblasts; Hematopoietic stem cells; Bone marrow; Stromal-derived factor-1; CXCR4; Prostate cancer.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000001

Control of the adrenocortical cell cycle: interaction between FGF2 and ACTH
Provedor de dados: 56
Autores: Armelin,H.A.; Lotfi,C.F.P.
FGF2 elicits a strong mitogenic response in the mouse Y-1 adrenocortical tumor cell line, that includes a rapid and transient activation of the ERK-MAPK cascade and induction of the c-Fos protein. ACTH, itself a very weak mitogen, blocks the mitogenic response effect of FGF2 in the early and middle G1 phase, keeping both ERK-MAPK activation and c-Fos induction at maximal levels. Probing the mitogenic response of Y-1 cells to FGF2 with ACTH is likely to uncover reactions underlying the effects of this hormone on adrenocortical cell growth.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenocortical cell cycle; ACTH; FGF2; ERK-MAPK; C-fos proto-oncogene.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700007

Serum and cerebrospinal fluid S100B concentrations in patients with neurocysticercosis
Provedor de dados: 56
Autores: Lima,J.E.; Walz,R.; Tort,A.; Souza,D.; Portela,L.; Bianchin,M.M.; Takayanagui,O.M.; Leite,J.P.
The clinical manifestations of neurocysticercosis (NC) are varied and depend on the number and location of cysts, as well as on the host immune response. Symptoms usually occur in NC when cysticerci enter a degenerative course associated with an inflammatory response. The expression of brain damage markers may be expected to increase during this phase. S100B is a calcium-binding protein produced and released predominantly by astrocytes that has been used as a marker of reactive gliosis and astrocytic death in many pathological conditions. The aim of the present study was to investigate the levels of S100B in patients in different phases of NC evolution. Cerebrospinal fluid and serum S100B concentrations were measured in 25 patients with NC: 14 patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurocysticercosis; S100B; Cerebrospinal fluid; Umin quotient; Glial marker.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100015

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001
Provedor de dados: 56
Autores: Beretta,A.L.R.Z.; Trabasso,P.; Stucchi,R.B.; Moretti,M.L.
Methicillin-resistant Staphylococcus aureus (MRSA) has been the cause of major outbreaks and epidemics among hospitalized patients, with high mortality and morbidity rates. We studied the genomic diversity of MRSA strains isolated from patients with nosocomial infection in a University Hospital from 1991 to 2001. The study consisted of two periods: period I, from 1991 to 1993 and period II from 1995 to 2001. DNA was typed by pulsed-field gel electrophoresis and the similarity among the MRSA strains was determined by cluster analysis. During period I, 73 strains presented five distinctive DNA profiles: A, B, C, D, and E. Profile A was the most frequent DNA pattern and was identified in 55 (75.3%) strains; three closely related and four possibly related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular epidemiology; Hospital infection; Pulsed-field gel electrophoresis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900009

TNF-α inhibits SCF, ghrelin, and substance P expressions through the NF-κB pathway activation in interstitial cells of Cajal
Provedor de dados: 56
Autores: Ren,Keyu; Yong,Chunming; Yuan,Hao; Cao,Bin; Zhao,Kun; Wang,Jin.
Ulcerative colitis is a chronic inflammatory disease of the colon where intestinal motility is disturbed. Interstitial cells of Cajal (ICC) are required to maintain normal intestinal motility. In the present study, we assessed the effect of tumor necrosis factor-alpha (TNF-α) on viability and apoptosis of ICC, as well as on the expression of stem cell factor (SCF), ghrelin, and substance P. ICC were derived from the small intestines of Swiss albino mice. Cell viability and apoptosis were measured using CCK-8 assay and flow cytometry, respectively. ELISA was used to measure the concentrations of IL-1β, IL-6, ghrelin, substance P, and endothelin-1. Quantitative RT-PCR was used to measure the expression of SCF. Western blotting was used to measure the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF-α; SCF; Ghrelin; Substance P; NF-κB; Interstitial cells of Cajal.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600610

Behavioral profiles displayed by rats in an elevated asymmetric plus-maze: effects of diazepam
Provedor de dados: 56
Autores: Ruarte,M.B.; Alvarez,E.O.
When rats are exposed to unknown environments where novelty and fear-inducing characteristics are present (conflictive environments), some specific behaviors are induced and exploration is apparently modulated by fear. In our laboratory, a new type of plus-maze was designed as a model of conflictive exploration. The maze is composed of four arms with different geometrical characteristics, differing from each other by the presence or absence of walls. The degree of asymmetry was as follows: NW, no wall arm; SW, a single high wall present; HL, a low and a high wall present, and HH, two high walls present. The four arms were arranged at 90o angles and the apparatus was called the elevated asymmetric plus-maze (APM). The purpose of the present study was to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emotionality; Anxiety; Motivation; Elevated asymmetric plus-maze; Benzodiazepine; Conflictive exploration.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100015

Bradykinin, insulin, and glycemia responses to exercise performed above and below lactate threshold in individuals with type 2 diabetes
Provedor de dados: 56
Autores: Asano,R.Y.; Browne,R.A.V.; Sales,M.M.; Arsa,G.; Moraes,J.F.V.N.; Coelho-Júnior,H.J.; Moraes,M.R.; Oliveira-Silva,I.; Atlas,S.E.; Lewis,J.E.; Simões,H.G.
The aim of this study was to analyze the acute responses of bradykinin, insulin, and glycemia to exercise performed above and below lactate threshold (LT) in individuals with type 2 diabetes mellitus (T2D). Eleven participants with a diagnosis of T2D randomly underwent three experimental sessions 72 h apart: 1) 20 min of exercise performed at 120% of LT (120%LT), 2) 20 min of exercise performed at 80% of LT (80%LT), and 3) 20 min of control session. Blood glucose was analyzed before, during, and at 45 min post-exercise. Bradykinin and insulin were analyzed before and at 45 min post-exercise. Both exercise sessions elicited a parallel decrease in glucose level during exercise (P≤0.002), with a greater decrease being observed for 120%LT (P=0.005). Glucose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic disease; Aerobic exercise; Peptide; Insulin resistance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100607

Development of fetal nicotine and muscarinic receptors in utero
Provedor de dados: 56
Autores: Mao,C.; Lv,J.; Li,H.; Chen,Y.; Wu,J.; Xu,Z.
The role of acetylcholine in the central and peripheral nervous systems is well established in adults. Cholinergic modulation of vascular functions and body fluid balance has been extensively studied. In the embryo-fetus, cholinergic receptors are widespread in the peripheral and central systems, including smooth muscle and the epithelial lining of the cardiovascular, digestive, and urinary systems, as well as in the brain. Fetal nicotine and muscarinic receptors develop in a pattern (e.g., amount and distribution) related to gestational periods. Cholinergic mechanisms have been found to be relatively intact and functional in the control of vascular homeostasis during fetal life in utero at least during the last third of gestation. This review focuses on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylcholine; Muscarinic/nicotinic receptors; Fetus; Swallowing; Vascular homeostasis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500019

Gene expression promoted by the SV40 DNA targeting sequence and the hypoxia-responsive element under normoxia and hypoxia
Provedor de dados: 56
Autores: Sacramento,C.B.; Moraes,J.Z.; Denapolis,P.M.A.; Han,S.W.
The main objective of the present study was to find suitable DNA-targeting sequences (DTS) for the construction of plasmid vectors to be used to treat ischemic diseases. The well-known Simian virus 40 nuclear DTS (SV40-DTS) and hypoxia-responsive element (HRE) sequences were used to construct plasmid vectors to express the human vascular endothelial growth factor gene (hVEGF). The rate of plasmid nuclear transport and consequent gene expression under normoxia (20% O2) and hypoxia (less than 5% O2) were determined. Plasmids containing the SV40-DTS or HRE sequences were constructed and used to transfect the A293T cell line (a human embryonic kidney cell line) in vitro and mouse skeletal muscle cells in vivo. Plasmid transport to the nucleus was monitored by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SV40-DTS; Hypoxia-responsive element; Transfection efficiency; Normoxia; Hypoxia; Ischemic diseases.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800005

Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense
Provedor de dados: 56
Autores: Sotomaior,P.; Araújo,L.M.; Nishikawa,C.Y.; Huergo,L.F.; Monteiro,R.A.; Pedrosa,F.O.; Chubatsu,L.S.; Souza,E.M.
Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azospirillum brasilense; NifA protein; GlnB protein; GAF domain; Nitrogen fixation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200005

Regulation of protein synthesis and the role of eIF3 in cancer
Provedor de dados: 56
Autores: Hershey,John W.B.
Maintenance of cell homeostasis and regulation of cell proliferation depend importantly on regulating the process of protein synthesis. Many disease states arise when disregulation of protein synthesis occurs. This review focuses on mechanisms of translational control and how disregulation results in cell malignancy. Most translational controls occur during the initiation phase of protein synthesis, with the initiation factors being the major target of regulation through their phosphorylation. In particular, the recruitment of mRNAs through the m7G-cap structure and the binding of the initiator methionyl-tRNAi are frequent targets. However, translation, especially of specific mRNAs, may also be regulated by sequestration into processing bodies or stress...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Translational control; EIF3; Malignant transformation; Cancer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000002

The role of chemokines and chemokine receptors in eosinophil activation during inflammatory allergic reactions
Provedor de dados: 56
Autores: Oliveira,S.H.P.; Lukacs,N.W.
Chemokines are important chemotactic cytokines that play a fundamental role in the trafficking of leukocytes to sites of inflammation. They are also potent cell-activating factors, inducing cytokine and histamine release and free radical production, a fact that makes them particularly important in the pathogenesis of allergic inflammation. The action of chemokines is regulated at the level of agonist production and processing as well as at the level of receptor expression and coupling. Therefore, an analysis of the ligands must necessarily consider receptors. Eosinophils are target cells involved in the allergic inflammatory response since they are able to release a wide variety of mediators including CC and CXC chemokines and express their receptors....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemokines; Chemokine receptors; Allergic inflammation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100002

Glass pipette-carbon fiber microelectrodes for evoked potential recordings
Provedor de dados: 56
Autores: Moraes,M.F.D.; Garcia-Cairasco,N.
Current methods for recording field potentials with tungsten electrodes make it virtually impossible to use the same recording electrode also as a lesioning electrode, for example for histological confirmation of the recorded site, because the lesioning procedure usually wears off the tungsten tip. Therefore, the electrode would have to be replaced after each lesioning procedure, which is a very high cost solution to the problem. We present here a low cost, easy to make, high quality glass pipette-carbon fiber microelectrode that shows resistive, signal/noise and electrochemical coupling advantages over tungsten electrodes. Also, currently used carbon fiber microelectrodes often show problems with electrical continuity, especially regarding electrochemical...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Evoked potentials; Glass pipette-carbon fiber; Microelectrodes; Voltammetry.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100010

Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
Provedor de dados: 56
Autores: Gonçalves,F.Z.; Lizarte Neto,F.S.; Novais,P.C.; Gattas,D.; Lourenço,L.G.; de Carvalho,C.A.M.; Tirapelli,D.P.C.; Molina,C.A.F.; Tirapelli,L.F.; Tucci Jr,S.
Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submitted to the experimental model of diabetes mellitus and chronic alcoholism. Forty-eight male Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D), and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study the protein expression of endothelin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholism; Corpus cavernosum; MiRNAs; Endothelin receptors.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300615

Prenatally programmed hypertension: role of maternal diabetes
Provedor de dados: 56
Autores: Gomes,G.N.; Gil,F.Z.
Epidemiological and experimental studies have led to the hypothesis of the fetal origin of adult diseases, suggesting that some adult diseases might be determined before birth by altered fetal development. Maternal diabetes subjects the fetus to an adverse environment that has been demonstrated to result in metabolic, cardiovascular and renal impairment in the offspring. The growing amount of obesity in young females in developed and some developing countries should contribute to increasing the incidence of diabetes among pregnant women. In this review, we discuss how renal and extrarenal mechanisms participate in the genesis of hypertension induced by a diabetic status during fetal development.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Maternal diabetes; Renal impairment; Sodium retention.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900011

IgE expression on the surface of B1 and B2 lymphocytes in experimental murine schistosomiasis
Provedor de dados: 56
Autores: Oliveira,F.L.; Aguiar,A.M.; Borojevic,R.; El-Cheikh,M.C.
In a previous study we monitored the distribution and phenotype expression of B1 cells during the evolution of experimental murine schistosomiasis mansoni and we proposed that the B1 cells were heterogeneous: a fraction which originated in the spleen and followed the migratory pathway to mesenteric ganglia, while the other was the resident peritoneal B1-cell pool. In the present study, we have addressed the question of whether these two B1-lymphocyte populations are involved in the production of the late Ig isotype IgE, which is present in high levels in schistosomal infection. Lymphocyte expression of surface markers and immunoglobulins were monitored by immunofluorescence flow cytometry. Both in the spleen and mesenteric ganglia, the B1 and B2 cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosomiasis; B lymphocytes; Immunoglobulin switch.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700006

Effect of thyroparathyroidectomy on urinary acidification in diabetic rats
Provedor de dados: 56
Autores: Zaladek-Gil,F.; Cavanal,M.F.; Nascimento-Gomes,G.; Silva-Rocha,M.C.; Nutti-de-Almeida,M.
In previous studies we have shown stimulation of renal acid excretion in the proximal tubules of rats with diabetes of short duration, with no important alterations in glomerular hemodynamics; on the other hand, in thyroparathyroidectomized rats (TPTX model), a significant decrease in renal acid excretion, glomerular filtration rate (GFR) and renal plasma flow (RPF) was detected. Since important changes in the parathyroid hormone-vitamin D-Ca axis are observed in the diabetic state, the present study was undertaken to investigate the renal repercussions of thyroparathyroidectomy in rats previously made diabetic by streptozotocin (45 mg/kg). Four to 6 days after the induction of diabetes (DM), a group of rats were thyroparathyroidectomized (DM + TPTX)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Thyroparathyroidectomy; Renal function; Acid excretion.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100016

Analysis of renin-angiotensin-aldosterone system gene polymorphisms in resistant hypertension
Provedor de dados: 56
Autores: Freitas,S.R.S.; Cabello,P.H.; Moura-Neto,R.S.; Dolinsky,L.C.; Lima,A.B.; Barros,M.; Bittencourt,I.; Cordovil,I.L.
Essential hypertension is a disease multifactorially triggered by genetic and environmental factors. The contribution of genetic polymorphisms of the renin-angiotensin-aldosterone system and clinical risk factors to the development of resistant hypertension was evaluated in 90 hypertensive patients and in 115 normotensive controls living in Southwestern Brazil. Genotyping for insertion/deletion of angiotensin-converting enzyme, angiotensinogen M235T, angiotensin II type 1 receptor A1166C, aldosterone synthase C344T, and mineralocorticoid receptor A4582C polymorphisms was performed by PCR, with further restriction analysis when required. The influence of genetic polymorphisms on blood pressure variation was assessed by analysis of the odds ratio, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential hypertension; Renin-angiotensin- aldosterone system; Polymorphisms; Genetics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300005

Cardiovascular, respiratory and metabolic responses to temperature and hypoxia of the winter frog Rana catesbeiana
Provedor de dados: 56
The objective of the present study was to determine the effects of hypoxia and temperature on the cardiovascular and respiratory systems and plasma glucose levels of the winter bullfrog Rana catesbeiana. Body temperature was maintained at 10, 15, 25 and 35oC for measurements of breathing frequency, heart rate, arterial blood pressure, metabolic rate, plasma glucose levels, blood gases and acid-base status. Reducing body temperature from 35 to 10oC decreased (P&amp;lt;0.001) heart rate (bpm) from 64.0 ± 3.1 (N = 5) to 12.5 ± 2.5 (N = 6) and blood pressure (mmHg) (P&amp;lt;0.05) from 41.9 ± 2.1 (N = 5) to 33.1 ± 2.1 (N = 6), whereas no significant changes were observed under hypoxia. Hypoxia-induced changes in breathing frequency and acid-base status were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Temperature; Hypoxia; Rana; Breathing frequency; Blood pressure; Heart rate; Acid-base status; Hyperglycemia.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100019

Control of gene expression in Trypanosomatidae
Provedor de dados: 56
Autores: Teixeira,S.M.R.
The study of mechanisms which control gene expression in trypanosomatids has developed at an increasing rate since 1989 when the first successful DNA transfection experiments were reported. Using primarily Trypanosoma brucei as a model, several groups have begun to elucidate the basic control mechanisms and to define the cellular factors involved in mRNA transcription, processing and translation in these parasites. This review focuses on the most recent studies regarding a subset of genes that are expressed differentially during the life cycle of three groups of parasites. In addition to T. brucei, I will address studies on gene regulation in a few species of Leishmania and the results obtained by a much more limited group of laboratories studying gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma; Leishmania; Gene expression; RNA; Antigenic variation; VSG.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001

Collagens and proteoglycans of the corneal extracellular matrix
Provedor de dados: 56
Autores: Michelacci,Y.M.
The cornea is a curved and transparent structure that provides the initial focusing of a light image into the eye. It consists of a central stroma that constitutes 90% of the corneal depth, covered anteriorly with epithelium and posteriorly with endothelium. Its transparency is the result of the regular spacing of collagen fibers with remarkably uniform diameter and interfibrillar space. Corneal collagen is composed of heterotypic fibrils consisting of type I and type V collagen molecules. The cornea also contains unusually high amounts of type VI collagen, which form microfibrillar structures, FACIT collagens (XII and XIV), and other nonfibrillar collagens (XIII and XVIII). FACIT collagens and other molecules, such as leucine-rich repeat proteoglycans,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornea; Proteoglycan; Glycosaminoglycan; Collagen; Dermatan sulfate; Keratan sulfate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800009

Localization of NMDA receptors in the cerebral cortex: a schematic overview
Provedor de dados: 56
The fundamental role of N-methyl-D-aspartate (NMDA) receptors in many cortical functions has been firmly defined, as has its involvement in a number of neurological and psychiatric diseases. However, until recently very little was known about the anatomical localization of NMDA receptors in the cerebral cortex of mammals. The recent application of molecular biological techniques to the study of NMDA receptors has provided specific tools which have greatly expanded our understanding of the localization of NMDA receptors in the cerebral cortex. In particular, immunocytochemical studies on the distribution of cortical NMDA receptors have shown that NMDA receptors are preferentially localized on dendritic spines, have disclosed an unknown fraction of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glutamate; Ionotropic receptors; Autoreceptors; Heteroreceptors; Astrocytes; Neuron-glia signaling.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500001

Role of the extracellular matrix in variations of invasive pathways in lung cancers
Provedor de dados: 56
Autores: de Sá,V.K.; Carvalho,L.; Gomes,A.; Alarcão,A.; Silva,M.R.; Couceiro,P.; Sousa,V.; Soares,F.A.; Capelozzi,V.L.
Among the most common features of highly invasive tumors, such as lung adenocarcinomas (AD) and squamous cell carcinomas (SqCC), is the massive degradation of the extracellular matrix. The remarkable qualitative and quantitative modifications of hyaluronidases (HAases), hyaluronan synthases (HAS), E-cadherin adhesion molecules, and the transforming growth factor β (TGF-β) may favor invasion, cellular motility, and proliferation. We examined HAase proteins (Hyal), HAS, E-cadherin, and TGF-β profiles in lung AD subtypes and SqCC obtained from smokers and non-smokers. Fifty-six patients, median age 64 years, who underwent lobectomy for AD (N = 31) and SqCC (N = 25) were included in the study. HAS-1, -2 and -3, and Hyal-1 and -3 were significantly more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung cancer E-cadherin TGF-β HAS-1; HAS-2 and HAS-3 Hyal-1 and Hyal-3 Immunohistochemistry Prognosis and morphometry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100021

Retinoic acid and cAMP inhibit rat hepatocellular carcinoma cell proliferation and enhance cell differentiation
Provedor de dados: 56
Autores: Ionta,M.; Rosa,M.C.; Almeida,R.B.; Freitas,V.M.; Rezende-Teixeira,P.; Machado-Santelli,G.M.
Hepatocellular carcinoma (HCC) is the third highest cause of cancer death worldwide. In general, the disease is diagnosed at an advanced stage when potentially curative therapies are no longer feasible. For this reason, it is very important to develop new therapeutic approaches. Retinoic acid (RA) is a natural derivative of vitamin A that regulates important biological processes including cell proliferation and differentiation. In vitro studies have shown that RA is effective in inhibiting growth of HCC cells; however, responsiveness to treatment varies among different HCC cell lines. The objective of the present study was to determine if the combined use of RA (0.1 µM) and cAMP (1 mM), an important second messenger, improves the responsiveness of HCC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell adhesion; CAMP; Differentiation; Proliferation; Hepatocarcinoma; Retinoic acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800006

TMEM16 proteins: the long awaited calcium-activated chloride channels?
Provedor de dados: 56
Autores: Flores,C.A.; Cid,L.P.; Sepúlveda,F.V.; Niemeyer,M.I.
Currents mediated by calcium-activated chloride channels (CaCCs), observed for the first time in Xenopus oocytes, have been recorded in many cells and tissues ranging from different types of neurons to epithelial and muscle cells. CaCCs play a role in the regulation of excitability in neurons including sensory receptors. In addition, they are crucial mediators of chloride movements in epithelial cells where their activity regulates electrolyte and fluid transport. The roles of CaCCs, particularly in epithelia, are briefly reviewed with emphasis on their function in secretory epithelia. The recent identification by three independent groups, using different strategies, of TMEM16A as the molecular counterpart of the CaCC is discussed. TMEM16A is part of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium-activated chloride channels; TMEM16; ANO; Intestinal secretion; Salivary gland; Lacrimal gland.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100001

Lima bean (Phaseolus lunatus) seed coat phaseolin is detrimental to the cowpea weevil (Callosobruchus maculatus)
Provedor de dados: 56
Autores: Moraes,R.A.; Sales,M.P.; Pinto,M.S.P.; Silva,L.B.; Oliveira,A.E.A.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.
The presence of phaseolin (a vicilin-like 7S storage globulin) peptides in the seed coat of the legume Phaseolus lunatus L. (lima bean) was demonstrated by N-terminal amino acid sequencing. Utilizing an artificial seed system assay we showed that phaseolin, isolated from both cotyledon and testa tissues of P. lunatus, is detrimental to the nonhost bruchid Callosobruchus maculatus (F) (cowpea weevil) with ED50 of 1.7 and 3.5%, respectively. The level of phaseolin in the seed coat (16.7%) was found to be sufficient to deter larval development of this bruchid. The expression of a C. maculatus-detrimental protein in the testa of nonhost seeds suggests that the protein may have played a significant role in the evolutionary adaptation of bruchids to legume seeds.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lima bean; Phaseolin (vicilin); 7S storage globulin; Seed coat; Cowpea weevil; Resistance to bruchids.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200005

Effects of reversible inactivation of the dorsomedial hypothalamus on panic- and anxiety-related responses in rats
Provedor de dados: 56
Autores: Nascimento,J.O.G.; Zangrossi Jr.,H.; Viana,M.B.
The medial hypothalamus is part of a neurobiological substrate controlling defensive behavior. It has been shown that a hypothalamic nucleus, the dorsomedial hypothalamus (DMH), is involved in the regulation of escape, a defensive behavior related to panic attacks. The role played by the DMH in the organization of conditioned fear responses, however, is less clear. In the present study, we investigated the effects of reversible inactivation of the DMH with the GABA A agonist muscimol on inhibitory avoidance acquisition and escape expression by male Wistar rats (approximately 280 g in weight) tested in the elevated T-maze (ETM). In the ETM, inhibitory avoidance, a conditioned defensive response, has been associated with generalized anxiety disorder. Results...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Muscimol; Generalized anxiety disorder; Panic disorder; Dorsomedial hypothalamus; Elevated T-maze.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900009

Targeting and translocation of endothelial nitric oxide synthase
Provedor de dados: 56
Autores: Michel,T.
This review explores advances in our understanding of the intracellular regulation of the endothelial isoform of nitric oxide synthase (eNOS) in the context of its dynamically regulated subcellular targeting. Nitric oxide (NO) is a labile molecule, and may play important biological roles both within the cell in which it is synthesized and in its interactions with nearby cells and molecules. The localization of eNOS within the cell importantly influences the biological role and chemical fate of the NO produced by the enzyme. eNOS, a Ca2+/calmodulin-dependent enzyme, is subject to a complex pattern of intracellular regulation, including co- and post-translational modifications and interactions with other proteins and ligands. In endothelial cells and cardiac...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caveolin; Signal transduction; Nitric oxide synthase; Calmodulin; Estrogen; Bradykinin.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100006

Clinical and pathological challenges in the diagnosis of late-onset biliary atresia: four case studies
Provedor de dados: 56
Autores: Fontenele,J.P.U.; Schenka,A.A.; Hessel,G.; Jarry,V.M.; Escanhoela,C.A.F.
Biliary atresia (BA) is classically described at the neonatal age. However, rare cases of BA in older infants have also been reported. We report four cases of late-onset BA in infants older than 4 weeks (3 males, 1 female), and describe the diagnostic and management difficulties. One of the cases had a late-onset (29 weeks) presentation with a successful surgical procedure. We highlight the importance of this unusual differential diagnosis in infants with cholestatic syndrome, who may benefit from Kasai surgery, regardless of age.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Kasai operation; Cholestatic syndrome; Infants.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300501

What about Th1/Th2 in cutaneous leishmaniasis vaccine discovery?
Provedor de dados: 56
Autores: Campos-Neto,A.
The T helper cell type 1 (Th1) response is essential to resist leishmaniasis, whereas the Th2 response favors the disease. However, many leishmanial antigens, which stimulate a Th1 immune response during the disease or even after the disease is cured, have been shown to have no protective action. Paradoxically, antigens associated with an early Th2 response have been found to be highly protective if the Th1 response to them is generated before infection. Therefore, finding disease-associated Th2 antigens and inducing a Th1 immune response to them using defined vaccination protocols is an interesting unorthodox alternative approach to the discovery of a leishmania vaccine.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Th1/Th2 paradigm; Infection; Vaccine; Antigen discovery; Adjuvant.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700001

Salivary cortisol and α-amylase: subclinical indicators of stress as cardiometabolic risk
Provedor de dados: 56
Autores: Cozma,S.; Dima-Cozma,L.C.; Ghiciuc,C.M.; Pasquali,V.; Saponaro,A.; Patacchioli,F.R.
Currently, the potential for cardiovascular (CV) stress-induced risk is primarily based on the theoretical (obvious) side effects of stress on the CV system. Salivary cortisol and α-amylase, produced respectively by the hypothalamus-pituitary-adrenal (HPA) axis and the sympathetic-adrenomedullary (SAM) system during stress response, are still not included in the routine evaluation of CV risk and require additional and definitive validation. Therefore, this article overviews studies published between 2010 and 2015, in which salivary cortisol and α-amylase were measured as stress biomarkers to examine their associations with CV/CMR (cardiometabolic risk) clinical and subclinical indicators. A comprehensive search of PubMed, Web of Science and Scopus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salivary stress biomarkers; Cardiometabolic risk (factors); Stress; Hypothalamic-pituitary-adrenal axis; Sympathetic adrenomedullary system.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200201

Rarity of DNA sequence alterations in the promoter region of the human androgen receptor gene
Provedor de dados: 56
Autores: Cabral,D.F.; Santos,A.; Ribeiro,M.L.; Mesquita,J.C.; Carvalho-Salles,A.B.; Hackel,C.
The human androgen receptor (AR) gene promoter lies in a GC-rich region containing two principal sites of transcription initiation and a putative Sp1 protein-binding site, without typical "TATA" and "CAAT" boxes. It has been suggested that mutations within the 5'untranslated region (5'UTR) may contribute to the development of prostate cancer by changing the rates of gene transcription and/or translation. In order to investigate this question, the aim of the present study was to search for the presence of mutations or polymorphisms at the AR-5'UTR in 92 prostate cancer patients, where histological diagnosis of adenocarcinoma was established in specimens obtained from transurethral resection or after prostatectomy. The AR-5'UTR was amplified by PCR from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human androgen receptor gene; Promoter region; Prostate cancer; 5'Untranslated region; DNA sequence alterations.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200004

Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity
Provedor de dados: 56
Autores: Mota,A.; Silva,P.; Neves,D.; Lemos,C.; Calhau,C.; Torres,D.; Martel,F.; Fraga,H.; Ribeiro,L.; Alçada,M.N.M.P.; Pinho,M.J.; Negrão,M.R.; Pedrosa,R.; Guerreiro,S.; Guimarães,J.T.; Azevedo,I.; Martins,M.J.
Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the p-nitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 ± 3.43 nmol p-nitrophenol·mg protein-1·min-1): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart; Alkaline phosphatase; Polyphenol-rich beverages; Steroid hormones; Methylxanthines.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700009

Correlation of serum leptin and insulin levels of pregnant protein-restricted rats with predictive obesity variables
Provedor de dados: 56
Autores: Macêdo,G.S.; Ferreira,C.L.P.; Menegaz,A.; Arantes,V.C.; Veloso,R.V.; Carneiro,E.M.; Boschero,A.C.; Oller do Nascimento,C.M.P.; Latorraca,M.Q.; Gomes-da-Silva,M.H.G.
During pregnancy and protein restriction, changes in serum insulin and leptin levels, food intake and several metabolic parameters normally result in enhanced adiposity. We evaluated serum leptin and insulin levels and their correlations with some predictive obesity variables in Wistar rats (90 days), up to the 14th day of pregnancy: control non-pregnant (N = 5) and pregnant (N = 7) groups (control diet: 17% protein), and low-protein non-pregnant (N = 5) and pregnant (N = 6) groups (low-protein diet: 6%). Independent of the protein content of the diet, pregnancy increased total (F1,19 = 22.28, P &lt; 0.001) and relative (F1,19 = 5.57, P &lt; 0.03) food intake, the variation of weight (F1,19 = 49.79, P &lt; 0.000) and final body weight (F1,19 = 19.52, P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low-protein diet; Pregnancy; Serum leptin; Serum insulin; Body adiposity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600014

Rescuing axons from degeneration does not affect retinal ganglion cell death
Provedor de dados: 56
Autores: de Lima,S.; Mietto,B.S.; Paula,C.; Muniz,T.; Martinez,A.M.B.; Gardino,P.F.
After a traumatic injury to the central nervous system, the distal stumps of axons undergo Wallerian degeneration (WD), an event that comprises cytoskeleton and myelin breakdown, astrocytic gliosis, and overexpression of proteins that inhibit axonal regrowth. By contrast, injured neuronal cell bodies show features characteristic of attempts to initiate the regenerative process of elongating their axons. The main molecular event that leads to WD is an increase in the intracellular calcium concentration, which activates calpains, calcium-dependent proteases that degrade cytoskeleton proteins. The aim of our study was to investigate whether preventing axonal degeneration would impact the survival of retinal ganglion cells (RGCs) after crushing the optic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optic nerve crush; Calpain inhibitor; Axon preservation; Axon degeneration; Retinal ganglion cells; Cell survival.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400705

Sedative and anesthetic activities of the essential oils of Hyptis mutabilis (Rich.) Briq. and their isolated components in silver catfish (Rhamdia quelen)
Provedor de dados: 56
Autores: Silva,L.L.; Garlet,Q.I.; Benovit,S.C.; Dolci,G.; Mallmann,C.A.; Bürger,M.E.; Baldisserotto,B.; Longhi,S.J.; Heinzmann,B.M.
This study evaluated the sedative and anesthetic effects of the essential oils (EO) of Hyptis mutabilis (Rich.) Briq. and their isolated components on silver catfish (Rhamdia quelen). Quantitative chemical differences between the EOs obtained from leaves and inflorescences were verified, and a new chemotype rich in globulol was described. Although there were no significant differences in the time of induction for sedation and anesthesia between the EOs, only the leaf EO at 344 mg/L anesthetized all fish without side effects. Fractionation of the leaf EO was carried out by column chromatography. The isolated compounds [(+)-1-terpinen-4-ol and (-)-globulol] showed different activity from that detected for the leaf EO in proportional concentrations and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver catfish; (+)-1-Terpinen-4-ol; (-)-Globulol; Sedation; GABA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900771

Cordyceps sinensis promotes immune regulation and enhances bacteriostatic activity of PA-824 via IL-10 in Mycobacterium tuberculosis disease
Provedor de dados: 56
Autores: Li,D.G.; Ren,Z.X.
PA-824 is a novel bicyclic nitroimidazole anti-tuberculosis (TB) drug. Cordyceps sinensis (Berk.) Sacc. (CS) was proven to be a good immunomodulatory compound. This research aimed to investigate the effect of CS on PA-824 in Mycobacterium tuberculosis (M.tb) infected mice (female CBA/J mice, 6 to 8 weeks of age and 20±2 g of weight). Mice were randomly assigned to 4 groups: PA-824, CS, PA-824+CS, and control. To verify the effect of PA-824 and CS on M.tb, after drug administration, mice lungs were harvested and bacterial colony formations were measured. Cells were isolated from infected lungs and spleens to analyze the percentage of CD4+ T cells (CD11a positive). Lung cells were cultured to detect the secretion of interferon-γ (IFN-γ) and interleukin-10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Cordyceps sinensis (Berk.) Sacc.; PA-824; IFN-γ; IL-10.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900604

Cultural adaptation and validation of the "Kidney Disease and Quality of Life - Short Form (KDQOL-SF™ 1.3)" in Brazil
Provedor de dados: 56
Autores: Duarte,P.S.; Ciconelli,R.M.; Sesso,R.
The objective of the present study was to translate the Kidney Disease Quality of Life - Short Form (KDQOL-SF™1.3) questionnaire into Portuguese to adapt it culturally and validate it for the Brazilian population. The KDQOL-SF was translated into Portuguese and back-translated twice into English. Patient difficulties in understanding the questionnaire were evaluated by a panel of experts and solved. Measurement properties such as reliability and validity were determined by applying the questionnaire to 94 end-stage renal disease patients on chronic dialysis. The Nottingham Health Profile Questionnaire, the Karnofsky Performance Scale and the Kidney Disease Questionnaire were administered to test validity. Some activities included in the original instrument...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney dialysis; Portuguese version of KDQOL-SF; End-stage renal disease; Health-related quality of life; KDQOL-SF; Test validation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200015

TNF-a and IL-10 levels in tracheobronchial lavage of ventilated preterm infants and subsequent lung function
Provedor de dados: 56
Autores: Corso,A.L.; Pitrez,P.M.C.; Machado,D.C.; Stein,R.T.; Jones,M.H.
The role of airway inflammation in ventilated preterm newborns and the risk factors associated with the development of chronic lung disease are not well understood. Our objective was to analyze the association of the airway inflammatory response in ventilated preterm infants by serial measurements of TNF-a and IL-10 in tracheobronchial lavage (TBL) with perinatal factors and lung function measured early in life. A series of TBL samples were collected from ventilated preterm infants (less than 32 weeks of gestational age) and concentrations of TNF-a and IL-10 were measured by ELISA. Pulmonary function tests were performed after discharge by the raised volume rapid compression technique. Twenty-five subjects were recruited and 70 TBL samples were obtained....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Newborn; Chronic lung disease; Interleukin-10; Tumor necrosis factor-a; Prematurity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400016

Two novel Mg(II)-based and Zn(II)-based complexes: inhibiting growth of human liver cancer cells
Provedor de dados: 56
Autores: Liu,N.; Ding,D.; Wang,L.; Zhao,H.; Zhu,L.; Geng,X.
Two new Mg(II)-based and Zn(II)-based coordination polymers, {[Mg3(BTB)(DMA)4](DMA)2}n (1, H3BTB=1,3,5-benzenetrisbenzoic acid, DMA=N,N-dimethylacetamide) and {(H2NMe2)2[Zn3(BTB)2(OH)(Im)](DMF)9(MeOH)7}n (2, Im=imidazole, DMF=N,N-dimethylformamide), have been successfully synthesized and structurally characterized under solvothermal conditions. 1 contains a linear [Mg3(COO)6] cluster that connected by the fully deprotonated BTB3- ligands to give a kgd-type 2D bilayer structure; 2 represents a microporous 3D pillar-layered system based on the binuclear Zn units and pillared Im ligands, which shows a (3,5)-connected hms topological net. In addition, in vitro anticancer activities of compounds 1 and 2 on 4 human liver cancer cells (HB611, HHCC, BEL-7405 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coordination polymer; Pillar-layered; Liver cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200614

Interruption of recently induced immune responses by oral administration of antigen
Provedor de dados: 56
Autores: Conde,A.A.; Stransky,B.; Faria,A.M.C.; Vaz,N.M.
Interest in oral tolerance has been renewed in the last few years as a possibility of intervention in human autoimmune diseases. An obstacle in this direction is that, although easily induced in animals virgin of contact with the antigen, oral tolerance becomes hard to induce in previously immunized animals. The present results show that there is an early period after primary immunization in which prolonged oral exposure to the antigen may arrest ongoing immune responses. Beyond this period, oral exposures to the antigen become ineffective and may actually boost immune responses. The end of the susceptible period coincides with the emergence of free specific antibodies in serum. However, the previous administration of purified anti-ovalbumin antibodies (40...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Suppression; Antibody response; Oral tolerance; Ovalbumin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300008

The Shiga toxin 2 B subunit inhibits net fluid absorption in human colon and elicits fluid accumulation in rat colon loops
Provedor de dados: 56
Autores: Creydt,V. Pistone; Miyakawa,M. Fernandez; Martín,F.; Zotta,E.; Silberstein,C.; Ibarra,C.
Shiga toxin (Stx)-producing Escherichia coli (STEC) colonizes the large intestine causing a spectrum of disorders, including watery diarrhea, bloody diarrhea (hemorrhagic colitis), and hemolytic-uremic syndrome. It is estimated that hemolytic-uremic syndrome is the most common cause of acute renal failure in infants in Argentina. Stx is a multimeric toxin composed of one A subunit and five B subunits. In this study we demonstrate that the Stx2 B subunit inhibits the water absorption (Jw) across the human and rat colonic mucosa without altering the electrical parameters measured as transepithelial potential difference and short circuit current. The time-course Jw inhibition by 400 ng/ml purified Stx2 B subunit was similar to that obtained using 12 ng/ml...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolytic-uremic syndrome; Diarrhea; Shiga toxin 2; B subunit; Water transport.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600004

Prokaryotic expression of a novel mouse pro-apoptosis protein PNAS-4 and application of its polyclonal antibodies
Provedor de dados: 56
Autores: Zhang,P.; Wang,C.T.; Yan,F.; Gou,L.; Tong,A.P.; Cai,F.; Li,Q.; Deng,H.X.; Wei,Y.Q.
Mouse PNAS-4 (mPNAS-4) has 96% identity with human PNAS-4 (hPNAS-4) in primary sequence and has been reported to be involved in the apoptotic response to DNA damage. However, there have been no studies reported of the biological functions of mPNAS-4. In studies conducted by our group (unpublished data), it was interesting to note that overexpression of mPNAS-4 promoted apoptotic death in Lewis lung carcinoma cells (LL2) and colon carcinoma cells (CT26) of mice both in vitro and in vivo. In our studies, mPNAS-4 was cloned into the pGEX-6P-1 vector with GST tag at N-terminal in Escherichia coli strain BL21(DE3). The soluble and insoluble expression of recombinant protein mPNAS-4 (rmPNAS-4) was temperature-dependent. The majority of rmPNAS-4 was insoluble at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-mPNAS-4; Polyclonal antibody; Apoptosis-related protein family; One-step affinity purification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600012

Co-culture of chondrocytes and bone marrow mesenchymal stem cells in vitro enhances the expression of cartilaginous extracellular matrix components
Provedor de dados: 56
Autores: Qing,Chang; Wei-ding,Cui; Wei-min,Fan.
Chondrocytes and bone marrow mesenchymal stem cells (BMSCs) are frequently used as seed cells in cartilage tissue engineering. In the present study, we determined if the co-culture of rabbit articular chondrocytes and BMSCs in vitro promotes the expression of cartilaginous extracellular matrix and, if so, what is the optimal ratio of the two cell types. Cultures of rabbit articular chondrocytes and BMSCs were expanded in vitro and then cultured individually or at a chondrocyte:BMSC ratio of 4:1, 2:1, 1:1, 1:2, 1:4 for 21 days and cultured in DMEM/F12. BMSCs were cultured in chondrogenic induction medium. Quantitative real-time RT-PCR and Western blot were used to evaluate gene expression. In the co-cultures, type II collagen and aggrecan expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-culture; Bone mesenchymal stem cells; Chondrocytes; Tissue engineering.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400006

Normal expression of IFN-gammaR in four patients with uncommon mycobacterial infection phenotypes
Provedor de dados: 56
Autores: Rugeles,M.T.; Rincón,B.; Rugeles,C.; Montoya,C.J.; Hernández,M.; Estrada,C.; Olivares,M.M.; Patiño,P.J.
Several primary immunodeficiency diseases affecting the interleukin 12/interferon gamma (IFN-gamma) pathway have been identified, most of them characterized by recurrent and protracted infections produced by intracellular microorganisms, particularly by several species of mycobacteria. In the present study we analyzed the expression of IFN-gamma receptor (IFN-gammaR) and signal transducer and activator of transcription 1 (STAT-1) in 4 children with Mycobacterium tuberculosis infection of uncommon clinical presentation. These molecules were evaluated by flow cytometry and Western blotting in B cells transformed with Epstein-Barr virus and mutations were scanned by single-strand conformational polymorphisms and DNA sequencing. The expression of IFN-gammaR1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterial disease; IFN-gamma; IFN-gamma receptor; STAT-1; SSCP-PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900010

Plasma lipid abnormalities in Pakistani population: trends, associated factors, and clinical implications
Provedor de dados: 56
Autores: Zaid,M.; Hasnain,S.
Previous studies have reported increased prevalence of coronary heart disease (CHD) in Indians and South Asian settlers in North America. This increased burden of CHD among South Asians is mainly caused by dyslipidemia. To the best of our knowledge, none of the previous works has studied the patterns and prevalence of dyslipidemia in the Pakistani population. The present work aimed to study the plasma lipid trends and abnormalities in a population-based sample of urban and rural Pakistanis. The study included 238 participants (108 males,130 females). Plasma lipid profiles of the participants were determined using standard protocols. We observed that 63% of the study population displayed irregularities in at least one major lipid-fraction including total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasma Lipids; Dyslipidemia; Coronary heart disease.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900609

The extracellular matrix provides directional cues for neuronal migration during cerebellar development
Provedor de dados: 56
Autores: Porcionatto,M.A.
Normal central nervous system development relies on accurate intrinsic cellular programs as well as on extrinsic informative cues provided by extracellular molecules. Migration of neuronal progenitors from defined proliferative zones to their final location is a key event during embryonic and postnatal development. Extracellular matrix components play important roles in these processes, and interactions between neurons and extracellular matrix are fundamental for the normal development of the central nervous system. Guidance cues are provided by extracellular factors that orient neuronal migration. During cerebellar development, the extracellular matrix molecules laminin and fibronectin give support to neuronal precursor migration, while other molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Central nervous system; Cerebellum; Neuron; Migration.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300001

Stimulated mast cells promote maturation of myocardial microvascular endothelial cell neovessels by modulating the angiopoietin-Tie-2 signaling pathway
Provedor de dados: 56
Autores: Wang,Z.H.; Zhu,W.; Tao,J.P.; Zhang,Q.Y.; Wei,M.
Angiopoietin (Ang)-1 and Ang-2 interact in angiogenesis to activate the Tie-2 receptor, which may be involved in new vessel maturation and regression. Mast cells (MCs) are also involved in formation of new blood vessels and angiogenesis. The present study was designed to test whether MCs can mediate angiogenesis in myocardial microvascular endothelial cells (MMVECs). Using a rat MMVEC and MC co-culture system, we observed that Ang-1 protein levels were very low even though its mRNA levels were increased by MCs. Interestingly, MCs were able to enhance migration, proliferation, and capillary-like tube formation, which were associated with suppressed Ang-2 protein expression, but not Tie-2 expression levels. These MCs induced effects that could be reversed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial microvascular endothelial cells; Mast cell; Angiogenesis; Angiopoietin; Tie-2.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100920

Control of the rat angiotensin I converting enzyme gene by CRE-like sequences
Provedor de dados: 56
Autores: Xavier-Neto,J.; Pereira,A.C.; Oliveira,E.M.; Miyakawa,A.A.; Junqueira,M.L.; Krieger,J.E.
We characterized the role of potential cAMP-responsive elements (CRE) in basal and in induced angiotensin converting enzyme (ACE) gene promoter activity in order to shed light on the regulation of somatic ACE expression. We identified stimulators and repressors of basal expression between 122 and 288 bp and between 415 and 1303 bp upstream from the transcription start site, respectively, using a rabbit endothelial cell (REC) line. These regions also contained elements associated with the response to 8BrcAMP. When screening for CRE motifs we found pCRE, a proximal sequence between 209 and 222 bp. dCRE, a distal tandem of two CRE-like sequences conserved between rats, mice and humans, was detected between 834 and 846 bp. Gel retardation analysis of nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin converting enzyme; Endothelium; CAMP; Cyclic AMP responsive element.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000002

Successful domino liver transplantation in maple syrup urine disease using a related living donor
Provedor de dados: 56
Autores: Feier,F.H.; Miura,I.K.; Fonseca,E.A.; Porta,G.; Pugliese,R.; Porta,A.; Schwartz,I.V.D.; Margutti,A.V.B.; Camelo Jr,J.S.; Yamaguchi,S.N.; Taveira,A.T.; Candido,H.; Benavides,M.; Danesi,V.; Guimaraes,T.; Kondo,M.; Chapchap,P.; Neto,J. Seda.
Maple syrup urine disease (MSUD) is an autosomal recessive disease associated with high levels of branched-chain amino acids. Children with MSUD can present severe neurological damage, but liver transplantation (LT) allows the patient to resume a normal diet and avoid further neurological damage. The use of living related donors has been controversial because parents are obligatory heterozygotes. We report a case of a 2-year-old child with MSUD who underwent a living donor LT. The donor was the patient's mother, and his liver was then used as a domino graft. The postoperative course was uneventful in all three subjects. DNA analysis performed after the transplantation (sequencing of the coding regions of BCKDHA, BCKDHB, andDBT genes) showed that the MSUD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterozygous donor; Metabolic disease; Branched-chain ketoacid dehydrogenase mutation; Leucine; Genotype.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600522

Identification and in vitro production of Lactobacillus antagonists from women with or without bacterial vaginosis
Provedor de dados: 56
Autores: Branco,K.M.G.R.; Nardi,R.M.D.; Moreira,J.L.S.; Nunes,A.C.; Farias,L.M.; Nicoli,J.R.; Carvalho,M.A.R.
Lactobacilli isolated from the vaginal tract of women with and without bacterial vaginosis (BV) were identified and characterized for the production of antagonists. Bacterial samples were isolated from healthy women (N = 16), from patients with clinical complaints but without BV (N = 30), and from patients with BV (N = 32). Identification was performed using amplified ribosomal DNA restriction analysis. Production of antagonistic compounds was evaluated by the double-layer diffusion technique using Gram-positive (N = 9) and Gram-negative bacteria (N = 6) as well as yeast (N = 5) as indicator strains. Of a total of 147 isolates, 133 were identified as pertaining to the genus Lactobacillus. Lactobacillus crispatus was the species most frequently recovered,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Bacterial vaginosis; Antagonism; Hydrogen peroxide; Identification.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400003

Biological X-ray irradiator characterization for use with small animals and cells
Provedor de dados: 56
Autores: Bruno,A. Colello; Mazaro,S.J.; Amaral,L.L.; Rego,E.M.; Oliveira,H.F.; Pavoni,J.F.
This study presents the characterization of an X-ray irradiator through dosimetric tests, which confirms the actual dose rate that small animals and cells will be exposed to during radiobiological experiments. We evaluated the linearity, consistency, repeatability, and dose distribution in the positions in which the animals or cells are placed during irradiation. In addition, we evaluated the performance of the X-ray tube (voltage and tube operating current), the radiometric survey (leakage radiation) and safety devices. The irradiator default setting was established as 160 kV and 25 mA. Tests showed that the dose rate was linear overtime (R2=1) and remained stable for long (constant) and short (repeatability) intervals between readings. The mean dose rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: X-ray irradiator; Dosimetric characterization; Radiotherapy; Dosimetry; Radiochromic film.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300607

Ethical research as the target of animal extremism: an international problem
Provedor de dados: 56
Autores: Michael Conn,P.; Rantin,F.T.
Animal extremism has been increasing worldwide; frequently researchers are the targets of actions by groups with extreme animal rights agendas. Sometimes this targeting is violent and may involve assaults on family members or destruction of property. In this article, we summarize recent events and suggest steps that researchers can take to educate the public on the value of animal research both for people and animals
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200001

Small-scale environmental enrichment and exercise enhance learning and spatial memory of Carassius auratus, and increase cell proliferation in the telencephalon: an exploratory study
Provedor de dados: 56
Autores: Abreu,C.C.; Fernandes,T.N.; Henrique,E.P.; Pereira,P.D.C.; Marques,S.B.; Herdeiro,S.L.S.; Oliveira,F.R.R.; Magalhães,N.G.M.; Anthony,D.C.; Melo,M.A.D.; Guerreiro-Diniz,C.; Diniz,D.G.; Picanço-Diniz,C.W.
Carassius auratus is a teleost fish that has been largely used in behavioral studies. However, little is known about potential environmental influences on its performance of learning and memory tasks. Here, we investigated this question in C. auratus, and searched for potential correlation between exercise and visuospatial enrichment with the total number of telencephalic glia and neurons. To that end, males and females were housed for 183 days in either an enriched (EE) or impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-hour/day water stream for voluntary exercise, whereas the IE had none of the above. A third plus-maze aquarium was used for spatial and object recognition tests. Different visual clues in 2 of its 4 arms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carassius auratus; Spatial learning and memory; Object recognition; Stereology; Telencephalon; Environmental enrichment.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500602

Genetic variability of hepatitis A virus isolates in Rio de Janeiro: implications for the vaccination of school children
Provedor de dados: 56
Autores: Villar,L.M.; Lampe,E.; Meyer,A.; Gaspar,A.M.C.
The epidemiology of hepatitis A virus (HAV) infection is shifting from high to intermediate endemicity in Brazil, resulting in increased numbers of susceptible individuals and a greater potential for the emergence of outbreaks. Universal vaccination against HAV has been recommended for children, but updated sero-epidemiological data are necessary to analyze the level of natural immunity and to identify candidates for preventive measures. In addition, more molecular studies are necessary to characterize the genotypes involved in HAV infections and outbreaks. Sera from 299 school children (5-15 years old) and 25 school staff members, collected during an outbreak of HAV at a rural public school in June 2000, were tested for IgM and total anti-HAV antibodies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis A virus (HAV); PCR; Molecular epidemiology; Sero-epidemiology; Vaccination.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200003

Chronic postnatal administration of methylmalonic acid provokes a decrease of myelin content and ganglioside N-acetylneuraminic acid concentration in cerebrum of young rats
Provedor de dados: 56
Autores: Brusque,A.M.; Rotta,L.; Pettenuzzo,L.F.; Junqueira,D.; Schwarzbold,C.V.; Wyse,A.T.; Wannmacher,C.M.D.; Dutra-Filho,C.S.; Wajner,M.
Levels of methylmalonic acid (MMA) comparable to those of human methylmalonic acidemia were achieved in blood (2-2.5 mmol/l) and brain (1.35 µmol/g) of rats by administering buffered MMA, pH 7.4, subcutaneously twice a day from the 5th to the 28th day of life. MMA doses ranged from 0.76 to 1.67 µmol/g as a function of animal age. Control rats were treated with saline in the same volumes. The animals were sacrificed by decapitation on the 28th day of age. Blood was taken and the brain was rapidly removed. Medulla, pons, the olfactory lobes and cerebellum were discarded and the rest of the brain ("cerebrum") was isolated. Body and "cerebrum" weight were measured, as well as the cholesterol and triglyceride concentrations in blood and the content of myelin,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methylmalonic acid; Glycerides; Lipids; Myelin; Rat brain; Methylmalonic acidemia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200010

Role of adenylyl cyclase in reduced β-adrenoceptor-mediated vasorelaxation during maturation
Provedor de dados: 56
Autores: López-Canales,O.A.; Castillo-Hernandez,M.C.; Vargas-Robles,H.; Rios,A.; López-Canales,J.S.; Escalante,B.
Beta-adrenergic receptor (βAR)-dependent blood vessel relaxation is impaired in older animals and G protein activation has been suggested as the causative mechanism. Here, we investigated the role of βAR subtypes (β1AR, β2AR, and β3AR) and cAMP in maturation-dependent vasorelaxation impairment. Aortic rings from 15 Sprague-Dawley male rats (3 or 9 weeks old) were harvested and left intact or denuded of the endothelium. Vascular relaxation in aortic rings from younger and older groups was compared in the presence of βAR subtype agonists and antagonists along with cAMP and cGMP antagonists. Isolated aortic rings were used to evaluate relaxation responses, protein expression was evaluated by western blot or real time PCR, and metabolites were measured by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β adrenergic receptors; Vascular tissue; Adenyl cyclase.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700605

Cytotoxicity of chlorhexidine digluconate to murine macrophages and its effect on hydrogen peroxide and nitric oxide induction
Provedor de dados: 56
Autores: Bonacorsi,C.; Raddi,M.S.G.; Carlos,I.Z.
Chlorhexidine, even at low concentrations, is toxic for a variety of eukaryotic cells; however, its effects on host immune cells are not well known. We evaluated in vitro chlorhexidine-induced cytotoxicity and its effects on reactive oxygen/nitrogen intermediate induction by murine peritoneal macrophages. Thioglycollate-induced cells were obtained from Swiss mice by peritoneal lavage with 5 ml of 10 mM phosphate-buffered saline, washed twice and resuspended (10(6) cells/ml) in appropriate medium for each test. Cell preparations contained more than 95% macrophages. The cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the presence of hydrogen peroxide (H2O2) and nitric oxide (NO) by the horseradish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorhexidine; Cytotoxicity; Macrophages; Nitric oxide; Hydrogen peroxide.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200007

Effect of oral ingestion of an extract of the herb Uncaria tomentosa on the biodistribution of sodium pertechnetate in rats
Provedor de dados: 56
Autores: Moreno,S.R.F.; Silva,A.L.C.; Diré,G.; Honeycut,H.; Carvalho,J.J.; Nascimento,A.L.; Pereira,M.; Rocha,E.K.; Oliveira-Timóteo,M.; Arnobio,A.; Olej,B.; Bernardo-Filho,M.; Caldas,L.Q.A.
The aim of the present study was to determine the effect of the oral ingestion of an extract of the herb Uncaria tomentosa (cat's claw) on the biodistribution of the radiobiocomplex sodium pertechnetate (Na99mTcO4) in rats. The animals (male Wistar rats, 2 months old, 180-220 g), were treated (1 mL) with an U. tomentosa extract (32 mg/mL, N = 5) or 0.9% NaCl solution (control, N = 5) for 7 days. After this period, Na99mTcO4 (3.7 MBq, 0.3 mL) was injected through the ocular plexus and after 10 min the rats were killed, the organs isolated and counted in a well-gamma counter. A significant (P &lt; 0.05) alteration in Na99mTcO4 uptake i) from 0.57 ± 0.008 to 0.39 ± 0.06 %ATI/organ (P &lt; 0.05) and from 0.57 ± 0.17 to 0.39 ± 0.14 %ATI/g (P &lt; 0.05) was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Uncaria tomentosa; Biodistribution; Sodium pertechnetate; Radiobiocomplex.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100010

Sleep-wake pattern of medical students: early versus late class starting time
Provedor de dados: 56
Autores: Lima,P.F.; Medeiros,A.L.D.; Araujo,J.F.
The sleep-wake cycle of students is characterized by delayed onset, partial sleep deprivation and poor sleep quality. Like other circadian rhythms, the sleep-wake cycle is influenced by endogenous and environmental factors. The aim of the present study was to determine the effects of different class starting times on the sleep-wake pattern of 27 medical students. The data were collected during two medical school semesters having different class starting times. All subjects answered the Portuguese version of the Horne and Östberg Morningness/Eveningness Questionnaire, the Pittsburgh Sleep Quality Index (PSQI) and kept a sleep diary for two weeks during each semester. Better sleep quality (PSQI = 5.3 vs 3.4), delayed sleep onset (23:59 vs 0:54 h) and longer...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sleep-wake cycle; Class schedule; Sleep quality; Sleep deprivation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100016

Evidence for the presence of a kininogen-like species in a case of total deficiency of low and high molecular weight kininogens
Provedor de dados: 56
Autores: Veloso,D.
Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178 &lt;FONT FACE="Symbol"&gt;®&lt;/FONT&gt; stop) has been blamed for the absence of LK and HK. However, using Western blots...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kininogen deficiency; Kinins; Antibodies; Plasma; Kininogen-like species; Human and nonhuman primates.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700004

Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Provedor de dados: 56
Autores: Nunes,V.A.; Gozzo,A.J.; Juliano,M.A.; Cerqueira César,M.; Sampaio,M.U.; Sampaio,C.A.M.; Araújo,M.S.
Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Caspase activation; Apoptosis; Dietary selenium deficiency; Dietary vitamin E deficiency; Internucleosomal DNA fragmentation; Caspase substrate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010

Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans
Provedor de dados: 56
Autores: Arantes,L.P.; Machado,M.L.; Zamberlan,D.C.; da Silveira,T.L.; da Silva,T.C.; da Cruz,I.B.M.; Ribeiro,E.E.; Aschner,M.; Soares,F.A.A.
Guarana (Paullinia cupana) is habitually ingested by people in the Amazon region and is a key ingredient in various energy drinks consumed worldwide. Extension in longevity and low prevalence of chronic age-related diseases have been associated to habitual intake of guarana. Anti-aging potential of guarana was also demonstrated in Caenorhabditis elegans; however, the mechanisms involved in its effects are not clear. Herein, we investigated the putative pathways that regulate the effects of guarana ethanolic extract (GEE) on lifespan using C. elegans. The major known longevity pathways were analyzed through mutant worms and RT-qPCR assay (DAF-2, DAF-16, SKN-1, SIR-2.1, HSF-1). The possible involvement of purinergic signaling was also investigated. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Antioxidant; Guarana; Lifespan; Natural products; Xanthines.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900602

Prenatal and postnatal depression among low income Brazilian women
Provedor de dados: 56
Autores: Da-Silva,V.A.; Moraes-Santos,A.R.; Carvalho,M.S.; Martins,M.L.P.; Teixeira,N.A.
Postnatal depression is a significant problem affecting 10-15% of mothers in many countries and has been the subject of an increasing number of publications. Prenatal depression has been studied less. The aims of the present investigation were: 1) to obtain information on the prevalence of prenatal and postnatal depression in low income Brazilian women by using an instrument already employed in several countries, i.e., the Edinburgh Postnatal Depression Scale (EPDS); 2) to evaluate the risk factors involved in prenatal and postnatal depression in Brazil. The study groups included 33 pregnant women interviewed at home during the second and third trimesters of pregnancy, and once a month during the first six months after delivery. Questions on life events...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depression; Pregnancy; Postnatal depression; Maternal depression.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600012

Use of fluorescent probes to follow membrane traffic in nerve terminals
Provedor de dados: 56
Autores: Guatimosim,C.; Romano-Silva,M.A.; Gomez,M.V.; Prado,M.A.M.
Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laser scanning confocal microscopy; FM1-43; Exocytosis; Synaptic vesicles; Endocytosis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018

Protection of plasmid DNA by a Ginkgo biloba extract from the effects of stannous chloride and the action on the labeling of blood elements with technetium-99m
Provedor de dados: 56
Autores: Moreno,S.R.F.; Freitas,R.S.; Rocha,E.K.; Lima-Filho,G.L.; Bernardo-Filho,M.
Ginkgo biloba extract (EGb) is a phytotherapeutic agent used for the treatment of ischemic and neurological disorders. Because the action of this important extract is not fully known, assays using different biological systems need to be performed. Red blood cells (RBC) are labeled with technetium-99m (Tc-99m) and used in nuclear medicine. The labeling depends on a reducing agent, usually stannous chloride (SnCl2). We assessed the effect of different concentrations of EGb on the labeling of blood constituents with Tc-99m, as sodium pertechnetate (3.7 MBq), and on the mobility of a plasmid DNA treated with SnCl2 (1.2 µg/ml) at room temperature. Blood was incubated with EGb before the addition of SnCl2 and Tc-99m. Plasma (P) and RBC were separated and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ginkgo biloba extract; Blood element labeling; Technetium-99m; Stannous chloride; Plasmid DNA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200015

Synergistic antitumor effect of TRAIL and adriamycin on the human breast cancer cell line MCF-7
Provedor de dados: 56
Autores: Cui,D.D.; Huang,Y.; Mao,S.H.; Chen,S.C.; Qiu,M.; Ji,L.L.; Yi,C.
The aim of the present study was to determine the effect of the combination of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and adriamycin (ADM) on the human breast cancer cell line MCF-7 and to identify potential mechanisms of apoptosis. Cell viability was analyzed by the MTT assay and the synergistic effect was assessed by the Webb coefficient. Apoptosis was quantified using the annexin V-FITC and propidium iodide staining flow cytometry. The mRNA expression of TRAIL receptors was measured by RT-PCR. Changes in the quantities of Bax and caspase-9 proteins were determined by Western blot. MCF-7 cells were relatively resistant to TRAIL (IC50 &gt;10 µg/mL), while MCF-7 cells were sensitive to ADM (IC50 &lt;10 µg/mL). A subtoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TRAIL; TRAIL receptors; Breast cancer; Adriamycin; Apoptosis; Synergism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900013

Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix
Provedor de dados: 56
Autores: Paez-Pereda,M.; Kuchenbauer,F.; Arzt,E.; Stalla,G.K.
The extracellular matrix is a three-dimensional network of proteins, glycosaminoglycans and other macromolecules. It has a structural support function as well as a role in cell adhesion, migration, proliferation, differentiation, and survival. The extracellular matrix conveys signals through membrane receptors called integrins and plays an important role in pituitary physiology and tumorigenesis. There is a differential expression of extracellular matrix components and integrins during the pituitary development in the embryo and during tumorigenesis in the adult. Different extracellular matrix components regulate adrenocorticotropin at the level of the proopiomelanocortin gene transcription. The extracellular matrix also controls the proliferation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Integrins; Pituitary gland; Pituitary adenomas; Pituitary hormones; Cell proliferation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000005

Gene transfer of Chlorella vulgaris n-3 fatty acid desaturase optimizes the fatty acid composition of human breast cancer cells
Provedor de dados: 56
Autores: Xue,Meilan; Ge,Yinlin; Zhang,Jinyu; Wang,Qing; Hou,Lin.
Chlorella vulgaris has the gene of n-3 fatty acid desaturase (CvFad3), which can synthesize the precursor of n-3 polyunsaturated fatty acids (PUFAs) or convert n-6 to n-3 PUFAs. The objective of the present study was to examine whether the CvFad3 gene from C. vulgaris can be functionally and efficiently expressed in human breast cancer cells and whether its expression can exert a significant effect on cell fatty acid composition. We inserted the CvFad3 gene into the plasmid pEGFP-C3 to construct the eukaryotic expression vector pEGFP-C3-n-3 and to express the n-3 Fad gene in human breast cancer cells (MCF-7 cells). Transfection of MCF-7 cells with the recombinant vector resulted in a high expression of n-3 fatty acid desaturase. Lipid analysis indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorella vulgaris; CvFad3 gene; Fatty acid desaturase; Recombinant expression vector; Fatty acid composition; Breast cancer cells.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200006

Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
Provedor de dados: 56
Autores: Mendes,G.A.; Haag,T.; Trott,G.; Rech,C.G.S.L.; Ferreira,N.P.; Oliveira,M.C.; Kohek,M.B.; Pereira-Lima,J.F.S.
Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acromegaly; Pituitary neoplasms; Cadherins; Slug; Neural cell adhesion molecules.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200605

Plasma amino acids in pregnancy, placental intervillous space and preterm newborn infants
Provedor de dados: 56
Autores: Camelo Jr.,J.S.; Martinez,F.E.; Gonçalves,A.L.; Monteiro,J.P.; Jorge,S.M.
Plasma amino acid levels have never been studied in the placental intervillous space of preterm gestations. Our objective was to determine the possible relationship between plasma amino acids of maternal venous blood (M), of the placental intervillous space (PIVS) and of the umbilical vein (UV) of preterm newborn infants. Plasma amino acid levels were analyzed by ion-exchange chromatography in M from 14 parturients and in the PIVS and UV of their preterm newborn infants. Mean gestational age was 34 ± 2 weeks, weight = 1827 ± 510 g, and all newborns were considered adequate for gestational age. The mean Apgar score was 8 and 9 at the first and fifth minutes. Plasma amino acid values were significantly lower in M than in PIVS (166%), except for aminobutyric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acid analysis; Placenta; Umbilical cord; Intervillous space; Maternal venous blood; Premature infants.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700012

Eradication of Helicobacter pylori infection in patients with duodenal ulcer and non-ulcer dyspepsia and analysis of one-year reinfection rates
Provedor de dados: 56
Autores: Della Libera,E.; Rohr,M.R.S.; Moraes,M.; Siqueira,E.S.; Ferrari Jr.,A.P.
Helicobacter pylori (HP) infection is endemic worldwide. The proposed treatment is expensive and there are few reports regarding reinfection rates in Brazil. The aim of this study was to compare the eradication rates obtained with two therapeutic options and to evaluate reinfection one year after treatment. This was a prospective randomized trial with 55 patients. Thirty-nine patients had active duodenal ulcer (DU) and 16 non-ulcer dyspepsia (NUD), and all tested positive for HP. Diagnosis was based on at least two positive tests: ultrarapid urease test, histology and/or culture. Patients were randomized to two groups: group OMC treated with 40 mg omeprazole (once a day), 500 mg metronidazole and 250 mg clarithromycin (twice daily) for 7 days, or group NA...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Helicobacter pylori; Peptic disease; Non-ulcer dyspepsia; Gastritis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600008

Coronary artery plaque burden and calcium scores in healthy men adhering to long-term wine drinking or alcohol abstinence
Provedor de dados: 56
Autores: da Luz,P.L.; Coimbra,S.; Favarato,D.; Albuquerque,C.; Mochiduky,R.I.; Rochitte,C.E.; Hojaij,E.; Gonsalves,C.R.L.; Laurindo,F.R.
Observational studies suggest there are clinical benefits to moderate red wine (RW) consumption. However, the effects on coronary vasculature and overall lifestyle are unclear. We investigated whether a lifestyle of regular long-term RW consumption is associated with changes in coronary plaque burden, calcium score, carotid intima/media thickness, endothelial function, and metabolic variables, compared with alcohol abstinence. Healthy volunteers were evaluated by coronary computed tomography angiography (CTA) as well as carotid and brachial artery ultrasound. Nutritional status, psychological status, and metabolic variables were assessed. The study included 101 drinkers [aged 58.9±7.3 years (means±SD)], from wine brotherhoods, and 104 abstainers, from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Red wine; Lifestyle; Computer tomography angiography; Coronary calcium; Carotid plaques.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800697

Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil
Provedor de dados: 56
Autores: Camargo,I.L.B.C.; Barth,A.L.; Pilger,K.; Seligman,B.G.S.; Machado,A.R.L.; Darini,A.L.C.
In 2000, Enterococcus faecalis resistant to vancomycin was first reported at a tertiary hospital in Porto Alegre, southern Brazil. The resistance spread to other hospitals and surveillance programs were established by hospital infection committees to prevent the spread of vancomycin-resistant enterococci. In February 2002, an isolate initially identified at the genus level as Enterococcus was obtained by surveillance culture (rectal swab) from a patient admitted to a hospital for treatment of septic arthritis in the shoulder. The isolate proved to be resistant to vancomycin by the disc diffusion method and confirmed by an E-test resulting in a minimal inhibitory concentration of  &gt; or = 256 µg/ml. This isolate was sent to a reference laboratory...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterococcus gallinarum; VanA phenotype; Vancomycin; Resistance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100012

Performance of urinary kidney injury molecule-1, neutrophil gelatinase-associated lipocalin, and N-acetyl-β-D-glucosaminidase to predict chronic kidney disease progression and adverse outcomes
Provedor de dados: 56
Autores: Lobato,G.R.; Lobato,M.R.; Thomé,F.S.; Veronese,F.V.
Urinary biomarkers can predict the progression of chronic kidney disease (CKD). In this study, kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), and N-acetyl-β-D-glucosaminidase (NAG) were correlated with the stages of CKD, and the association of these biomarkers with CKD progression and adverse outcomes was determined. A total of 250 patients, including 111 on hemodialysis, were studied. Urinary KIM-1, NGAL, and NAG were measured at baseline. Patients not on dialysis at baseline who progressed to a worse CKD stage were compared with those who did not progress. The association of each biomarker and selected covariates with progression to more advanced stages of CKD, end-stage kidney disease, or death was evaluated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney disease; Hemodialysis; Biomarkers; Kidney injury molecule-1; Neutrophil gelatinase-associated lipocalin; N-acetyl-β-D-glucosaminidase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500702

Developed pressure data may provide misinformation when used alone to evaluate systolic function in isovolumetric left ventricle preparations
Provedor de dados: 56
Autores: Murad,N.; Tucci,P.J.F.
We report data showing that developed pressure (DPmax) may lead to opposite conclusion with respect to maximal developed circumferential wall stress (&lt;FONT FACE="Symbol"&gt;s&lt;/FONT&gt;max) when used to assess contractile function in left ventricle isovolumic preparations. Isovolumetric left ventricle preparations of rats with cardiac hypertrophy (H; N = 10) induced by isoproterenol administration showed higher DPmax (174 ± 14 mmHg) than control (C; N = 8) animals (155 ± 12 mmHg) or rats with regression (R; N = 8) of hypertrophy (144 ± 11 mmHg). In contrast, the estimated &lt;FONT FACE="Symbol"&gt;s&lt;/FONT&gt;max for C (145 ± 26 kdynes/cm2) and R (133 ± 17 kdynes/cm2) was higher than for H (110 ± 13 kdynes/cm2). According to Laplace's law, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Contractile function; Developed pressure; Wall stress; Cardiac hypertrophy; Laplace's law.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600014

Identification of hemolytic and neuroactive fractions in the venom of the sea anemone Bunodosoma cangicum
Provedor de dados: 56
Autores: Lagos,P.; Duran,R.; Cerveñansky,C.; Freitas,J.C.; Silveira,R.
Sea anemones are a rich source of biologically active substances. In crayfish muscle fibers, Bunodosoma cangicum whole venom selectively blocks the I K(Ca) currents. In the present study, we report for the first time powerful hemolytic and neuroactive effects present in two different fractions obtained by gel-filtration chromatography from whole venom of B. cangicum. A cytolytic fraction (Bcg-2) with components of molecular mass ranging from 8 to 18 kDa elicited hemolysis of mouse erythrocytes with an EC50 = 14 µg/ml and a maximum dose of 22 µg/ml. The effects of the neuroactive fraction, Bcg-3 (2 to 5 kDa), were studied on isolated crab nerves. This fraction prolonged the compound action potentials by increasing their duration and rise time in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bunodosoma cangicum; Sea anemone; Hemolytic activity; Neurotoxins; Crab nerve.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700009

An MD-PhD program in Brazil: students’ concepts of science and of common sense
Provedor de dados: 56
Autores: Oliveira,R.V.; Campos,P.C.C.; Mourão,P.A.S.
In 1995, a pioneering MD-PhD program was initiated in Brazil for the training of medical scientists in experimental sciences at the Federal University of Rio de Janeiro. The program’s aim was achieved with respect to publication of theses in the form of papers with international visibility and also in terms of fostering the scientific careers of the graduates. The expansion of this type of program is one of the strategies for improving the preparation of biomedical researchers in Brazil. A noteworthy absence of interest in carrying out clinical research limits the ability of young Brazilian physicians to solve biomedical problems. To understand the students’ views of science, we used qualitative and quantitative triangulation methods, as well as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: MD-PhD program evaluation; Concept of science; Common sense concept; Medical research training; Methodological triangulation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100005

Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols
Provedor de dados: 56
Autores: de Moraes,F.M.; Espósito,D.L.A.; Klein,T.M.; da Fonseca,B.A.L.
Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. To reach the best possible Zika diagnosis, the analytic sensitivity of some probe-based real-time RT-PCR amplifying Zika virus RNA was evaluated in spiked and clinical samples. We evaluated primers and probes to detect Zika virus, which had been published before, and tested sensitivity using serum spiked and patient samples by real-time RT-PCR....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika virus; Flavivirus; Real time RT-PCR; Diagnosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600618

Local treatment with ascorbic acid accelerates recovery of post-sutured Achilles tendon in male Wistar rats
Provedor de dados: 56
Autores: Souza,M.; Moraes,S.A.S.; de Paula,D.R.; Maciel,A.A.; Batista,E.J.O.; Silva,D.G.F.; Bahia,C.P.; Oliveira,K.R.H.M.; Herculano,A.M.
Tendon rupture is a very frequent accident involving average people and high-performance athletes. Clinical studies describe tendon recovery as a painful and slow process involving different biochemical and histological events. Ascorbic acid (AA) is a potent antioxidant as well as an important cofactor for collagen synthesis. In the current study, we evaluated if local treatment with AA is able to promote tendon repair in tenotomized rats. Animals were submitted to Achilles tendon rupture followed by surgical suture. Control and AA groups received in loco injection of saline solution (0.9% NaCl) and 30 mM AA, respectively. Histological and functional recovery of Achilles tendon tissue was evaluated at 7, 14, and 21 days post-surgery. Hematoxylin/eosin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tendon recovery; Tenotomy; Ascorbic acid; Tendon suture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900608

Comparison of totally tubeless percutaneous nephrolithotomy and standard percutaneous nephrolithotomy for kidney stones: a randomized, clinical trial
Provedor de dados: 56
Autores: Moosanejad,N.; Firouzian,A.; Hashemi,S.A.; Bahari,M.; Fazli,M.
This study aimed to compare the totally tubeless percutaneous nephrolithotomy and standard percutaneous nephrolithotomy techniques regarding their rates of success and complications in patients with kidney stones. Patients were randomly assigned to two groups. Forty-four patients (24 men; mean age: 50.40±2.02 years) received totally tubeless percutaneous nephrolithotomy (PCNL; no nephrostomy catheter or ureteral catheter after PCNL) and 40 patients (18 men; mean age: 49.95±13.38 years) underwent standard PCNL (a nephrostomy catheter and ureteral catheter were used after PCNL). All surgeries were performed by one surgeon. Postoperative changes in hemoglobin, the blood transfusion rate, changes in creatinine levels, operation time, analgesic need,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Totally tubeless percutaneous nephrolithotomy; Standard percutaneous nephrolithotomy; Complications.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400704

Total phenolic, flavonoid, alkaloid and iridoid content and preventive effect of Lider-7-tang on lipopolysaccharide-induced acute lung injury in rats
Provedor de dados: 56
Autores: Erdenechimeg,Ch.; Guiqide,A.; Dejidmaa,B.; Chimedragchaa,Ch.; Purevsuren,S.
Lider-7-tang, a medicine used for the treatment of respiratory diseases especially pneumonia and fever in Mongolian Traditional Medicine, was selected for this phytochemical and pharmacological study. The objectives of the study were to determine total biological active substances and analyze the effects of Lider-7-tang treatment in rats with acute lung injury (ALI). Quantitative determination of the total active constituents (phenolic, flavonoid, iridoid and alkaloid) of the methanol extract of Lider-7-tang was performed using Folin-Ciocalteu reagent, aluminum chloride reagent, Trim-Hill reagent, and Bromocresol green reagent, respectively. A total of fifty 8–10-week-old male Wistar rats (200–240 g) were randomized into three groups: control group,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Traditional medicine; Lider-7-tang; Phenolic; Acute lung injury; Lipopolysaccharide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200609

Association between dental-oral health in young adults and salivary glutathione, lipid peroxidation and sialic acid levels and carbonic anhydrase activity
Provedor de dados: 56
Autores: Öztürk,L.K.; Furuncuoğlu,H.; Atala,M.H.; Uluköylü,O.; Akyüz,S.; Yarat,A.
The aim of the present study was to evaluate the relationship between salivary oxidative stress and dental-oral health. Healthy young adults, matched for gender and age, with (N = 21, 10 men, mean age: 20.3 ± 1 years) and without (N = 16, 8 men, mean age: 21.2 ± 1.8 years) caries were included in this study. The World Health Organization (WHO) caries diagnostic criteria were used for determining the decayed, missing, filled teeth (DMFT) index. The oral hygiene and gingival status were assessed using the simplified oral hygiene index and gingival index, respectively. Unstimulated salivary total protein, glutathione (GSH), lipid peroxidation and total sialic acid levels, carbonic anhydrase activity, and salivary buffering capacity were determined by standard...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glutathione; Lipid peroxidation; Sialic acid; Carbonic anhydrase; Dental-oral health.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100003

Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region
Provedor de dados: 56
Autores: Gong,Zheng; Luo,Qi-Zhi; Lin,Lin; Su,Yu-Ping; Peng,Hai-Bo; Du,Kun; Yu,Ping; Wang,Shi-Ping.
Major histocompatibility complex class I chain-related A (MICA) is a highly polymorphic gene located within the MHC class I region of the human genome. Expressed as a cell surface glycoprotein, MICA modulates immune surveillance by binding to its cognate receptor on natural killer cells, NKG2D, and its genetic polymorphisms have been recently associated with susceptibility to some infectious diseases. We determined whether MICA polymorphisms were associated with the high rate of Schistosoma parasitic worm infection or severity of disease outcome in the Dongting Lake region of Hunan Province, China. Polymerase chain reaction-sequence specific priming (PCR-SSP) and sequencing-based typing (SBT) were applied for high-resolution allele typing of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma japonicum; MICA; NKG2D; Gene polymorphism; Liver fibrosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300007

Smoking and psychiatric disorders: a comorbidity survey
Provedor de dados: 56
Autores: Lopes,F.L.; Nascimento,I.; Zin,W.A.; Valença,A.M.; Mezzasalma,M.A.; Figueira,I.; Nardi,A.E.
Epidemiological and clinical studies have shown a positive correlation between smoking and psychiatric disorders. To investigate the prevalence of cigarette smoking, 277 psychiatric outpatients with anxiety or depressive disorders (DSM-IV) answered a self-evaluation questionnaire about smoking behavior and were compared with a group of 68 control subjects. The diagnoses (N = 262) were: 30.2% (N = 79) major depressive disorder, 23.3% (N = 61) panic disorder, 15.6% (N = 41) social anxiety disorder, 7.3% (N = 19) other anxiety disorders, and 23.7% (N = 62) comorbidity disorders. Among them, 26.3% (N = 69) were smokers, 23.7% (N = 62) were former smokers and 50.0% (N = 131) were nonsmokers. The prevalence of nicotine dependence among the smokers was 59.0%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smoking; Panic disorder; Social anxiety disorder; Depression; Depressive disorder.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800013

Detection of point mutations by non-isotopic single strand conformation polymorphism
Provedor de dados: 56
Autores: Pinheiro,N.A.; Moura,R.P.; Monteiro,E.; Villa,L.L.
We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: P53 gene; Human papillomavirus; PCR; SSCP; Cancer; Silver staining.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100008

Ectonucleotidase activities in Sertoli cells from immature rats
Provedor de dados: 56
Autores: Casali,E.A.; Silva,T.R. da; Gelain,D.P.; Kaiser,G.R.R.F.; Battastini,A.M.O.; Sarkis,J.J.F.; Bernard,E.A.
Sertoli cells have been shown to be targets for extracellular purines such as ATP and adenosine. These purines evoke responses in Sertoli cells through two subtypes of purinoreceptors, P2Y2 and P A1. The signals to purinoreceptors are usually terminated by the action of ectonucleotidases. To demonstrate these enzymatic activities, we cultured rat Sertoli cells for four days and then used them for different assays. ATP, ADP and AMP hydrolysis was estimated by measuring the Pi released using a colorimetric method. Adenosine deaminase activity (EC 3.5.4.4) was determined by HPLC. The cells were not disrupted after 40 min of incubation and the enzymatic activities were considered to be ectocellularly localized. ATP and ADP hydrolysis was markedly increased by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sertoli cells; Purine nucleotides; ATP diphosphohydrolase; Ecto-5'-nucleotidase; Ectoadenosine deaminase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000003

SULF2 promotes tumorigenesis and inhibits apoptosis of cervical cancer cells through the ERK/AKT signaling pathway
Provedor de dados: 56
Autores: Jiang,Tao; Chen,Zhao-Hui; Chen,Zhe; Tan,Dan.
The objective of this study was to explore the role of the SULF2-mediated ERK/AKT signaling pathway in cervical cancer. SULF2 expression was detected in tumor tissues and tumor-adjacent normal tissues from cervical cancer patients. HeLa cells were divided into six groups: control group, NC group, SULF2 siRNA group, SULF2 group, SULF2 + LY294002 group, and SULF2 + U0125 group. In each group, HeLa cells received the corresponding treatment, followed by measurement of the cellular biological characteristics and expression of the ERK/AKT signaling pathway. We also confirmed the effect of SULF2 in vivo using a xenograft model in nude mice. SULF2 was upregulated in cervical cancer tissues, which was specifically associated with the clinical stage, histological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SULF2; Cervical cancer; ERK/AKT; Proliferation; Apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200608

Laboratory evidence for the hematopoietic potential of Beta vulgaris leaf and stalk extract in a phenylhydrazine model of anemia
Provedor de dados: 56
Autores: Gheith,I.; El-Mahmoudy,A.
This study was designed to provide laboratory evidence supporting the hematopoietic effect of Beta vulgaris (beet) leaf aqueous extract in phenylhydrazine-induced anemia model in albino rats. Extraction of the leaves/stalks was done by maceration in 30% hydro-ethanol for 48 h. An intraperitoneal injection of 20 mg/kg phenylhydrazine was applied for two consecutive days to develop hemolytic anemia on the 4th day after the 1st injection in 24 of 30 male albino rats. The animals were divided into 5 groups and received the following treatments: standard (ferrous ascorbate + folic acid; 13.5 + 0.135 mg/kg), B. vulgaris extract (100 and 200 mg/kg), or left untreated (normal and diseased controls). Blood samples were taken at 0, 4, 8, and 12 days of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anemia; Beta vulgaris; Beet; Hematinic; Anti-anemic; Antioxidant.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100611

Vasopressor mechanisms in acute aortic coarctation hypertension
Provedor de dados: 56
Angiotensin II (ANG II) and vasopressin (AVP) act together with the mechanical effect of aortic constriction in the onset of acute aortic coarctation hypertension. Blockade of ANG II and AVP V1 receptors demonstrated that ANG II acts on the prompt (5 min) rise in pressure whereas AVP is responsible for the maintenance (30-45 min) of the arterial pressure elevation during aortic coarctation. Hormone assays carried out on blood collected from conscious rats submitted to aortic constriction supported a role for ANG II in the early stage and a combined role for both ANG II and AVP in the maintenance of proximal hypertension. As expected, a role for catecholamines was ruled out in this model of hypertension, presumably due to the inhibitory effect of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aortic coarctation; Vasopressin; Angiotensin; Renal afferences; Hypertension.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400003

A novel frameshift mutation of Chediak-Higashi syndrome and treatment in the accelerated phase
Provedor de dados: 56
Autores: Wu,X.L.; Zhao,X.Q.; Zhang,B.X.; Xuan,F.; Guo,H.M.; Ma,F.T.
Chediak-Higashi syndrome (CHS) is a rare autosomal recessive immunodeficiency disease characterized by frequent infections, hypopigmentation, progressive neurologic deterioration and hemophagocytic lymphohistiocytosis (HLH), known as the accelerated phase. There is little experience in the accelerated phase of CHS treatment worldwide. Here, we present a case of a 9-month-old boy with continuous high fever, hypopigmentation of the skin, enlarged lymph nodes, hepatosplenomegaly and lung infection. He was diagnosed with CHS by gene sequencing, and had entered the accelerated phase. After 8 weeks of therapy, the boy had remission and was prepared for allogenic stem cell transplantation.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Chediak-Higashi syndrome; Accelerated phase; Gene sequencing; Therapy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400501

Moderate-intensity exercise allows enhanced protection against oxidative stress-induced cardiac dysfunction in spontaneously hypertensive rats
Provedor de dados: 56
Autores: Mi,Chunjuan; Qin,Xinghua; Hou,Zuoxu; Gao,Feng.
The progression of myocardial injury secondary to hypertension is a complex process related to a series of physiological and molecular factors including oxidative stress. This study aimed to investigate whether moderate-intensity exercise (MIE) could improve cardiac function and oxidative stress in spontaneously hypertensive rats (SHRs). Eight-week-old male SHRs and age-matched male Wistar-Kyoto rats were randomly assigned to exercise training (treadmill running at a speed of 20 m/min for 1 h continuously) or kept sedentary for 16 weeks. Cardiac function was monitored by polygraph; cardiac mitochondrial structure was observed by scanning electron microscope; tissue free radical production was measured using dihydroethidium staining. Expression levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; SHR; Oxidative stress; SIRT3/SOD2; Mitochondria function.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600603

Cytoskeletal and cellular adhesion proteins in zebrafish (Danio rerio) myogenesis
Provedor de dados: 56
Autores: Costa,M.L.; Escaleira,R.; Manasfi,M.; Souza,L.F. de; Mermelstein,C.S.
The current myogenesis and myofibrillogenesis model has been based mostly on in vitro cell culture studies, and, to a lesser extent, on in situ studies in avian and mammalian embryos. While the more isolated artificial conditions of cells in culture permitted careful structural analysis, the actual in situ cellular structures have not been described in detail because the embryos are more difficult to section and manipulate. To overcome these difficulties, we used the optically clear and easy to handle embryos of the zebrafish Danio rerio. We monitored the expression of cytoskeletal and cell-adhesion proteins (actin, myosin, desmin, alpha-actinin, troponin, titin, vimentin and vinculin) using immunofluorescence microscopy and video-enhanced,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytoskeleton; Cell adhesion; Zebrafish; Myogenesis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800019

Participation of hepatic α/β-adrenoceptors and AT1 receptors in glucose release and portal hypertensive response induced by adrenaline or angiotensin II
Provedor de dados: 56
Autores: de Araújo,L.J.T.; Nagaoka,M.R.; Borges,D.R.; Kouyoumdjian,M.
It has been previously demonstrated that the hemodynamic effect induced by angiotensin II (AII) in the liver was completely abolished by losartan while glucose release was partially affected by losartan. Angiotensin II type 1 (AT1) and adrenergic (∝1- and β-) receptors (AR) belong to the G-proteins superfamily, which signaling promote glycogen breakdown and glucose release. Interactive relationship between AR and AT1-R was shown after blockade of these receptors with specific antagonists. The isolated perfused rat liver was used to study hemodynamic and metabolic responses induced by AII and adrenaline (Adr) in the presence of AT1 (losartan) and ∝1-AR and β-AR antagonists (prazosin and propranolol). All antagonists diminished the hemodynamic response...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver perfusion; Angiotensin II; Adrenaline; AT1R; Adrenoceptors.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200604

Stem cell factor protects against neuronal apoptosis by activating AKT/ERK in diabetic mice
Provedor de dados: 56
Autores: Li,J.-W.; Li,L.-L.; Chang,L.-L.; Wang,Z.-Y.; Xu,Y.
Neuronal apoptosis occurs in the diabetic brain due to insulin deficiency or insulin resistance, both of which reduce the expression of stem cell factor (SCF). We investigated the possible involvement of the activation of the MAPK/ERK and/or AKT pathways in neuroprotection by SCF in diabetes. Male C57/B6 mice (20-25 g) were randomly divided into four groups of 10 animals each. The morphology of the diabetic brain in mice treated or not with insulin or SCF was evaluated by H&amp;E staining and TUNEL. SCF, ERK1/2 and AKT were measured by Western blotting. In diabetic mice treated with insulin or SCF, there was fewer structural change and apoptosis in the cortex compared to untreated mice. The apoptosis rate of the normal group, the diabetic group receiving...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stem cell factor; Diabetes; MAPK/ERK; AKT.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100008

Modulation of hormone secretion by functional electrical stimulation of the intact and incompletely dysfunctional dog pancreas
Provedor de dados: 56
Autores: Rozman,J.; Bunc,M.; Zorko,B.
The purpose of the present study was to modulate the secretion of insulin and glucagon in Beagle dogs by stimulation of nerves innervating the intact and partly dysfunctional pancreas. Three 33-electrode spiral cuffs were implanted on the vagus, splanchnic and pancreatic nerves in each of two animals. Partial dysfunction of the pancreas was induced with alloxan. The nerves were stimulated using rectangular, charge-balanced, biphasic, and constant current pulses (200 µs, 1 mA, 20 Hz, with a 100-µs delay between biphasic phases). Blood samples from the femoral artery were drawn before the experiment, at the beginning of stimulation, after 5 min of stimulation, and 5 min after the end of stimulation. Radioimmunoassay data showed that in the intact pancreas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional electrical stimulation; Implanted electrodes; Autonomic nerves; Pancreatic secretion; Insulin; Glucagon.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300012

Modulation of intercellular communication by differential regulation and heteromeric mixing of co-expressed connexins
Provedor de dados: 56
Autores: Beyer,E.C.; Gemel,J.; Seul,K.H.; Larson,D.M.; Banach,K.; Brink,P.R.
Intercellular communication may be regulated by the differential expression of subunit gap junction proteins (connexins) which form channels with differing gating and permeability properties. Endothelial cells express three different connexins (connexin37, connexin40, and connexin43) in vivo. To study the differential regulation of expression and synthesis of connexin37 and connexin43, we used cultured bovine aortic endothelial cells which contain these two connexins in vitro. RNA blots demonstrated discordant expression of these two connexins during growth to confluency. RNA blots and immunoblots showed that levels of these connexins were modulated by treatment of cultures with transforming growth factor-ß1. To examine the potential ability of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelium; Heteromeric connexons; Intercellular communication; Connexin37; Connexin40.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400004

Tissue hyaluronan expression, as reflected in the sputum of lung cancer patients, is an indicator of malignancy
Provedor de dados: 56
Autores: Rangel,M.P.; de Sá,V.K.; Martins,V.; Martins,J.R.M.; Parra,E.R.; Mendes,A.; Andrade,P.C.; Reis,R.M.; Longatto-Filho,A.; Oliveira,C.Z.; Takagaki,T.; Carraro,D.M.; Nader,H.B.; Capelozzi,V.L.
Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronic acid; Sputum; Lung cancer; Immunohistochemistry; ELISA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600557

Hypertensive effects of the iv administration of picomoles of ouabain
Provedor de dados: 56
Autores: Padilha,A.S.; Salaices,M.; Vassallo,D.V.; Batista,P.R.; Siman,F.D.M.
Ouabain, an endogenous digitalis compound, has been detected in nanomolar concentrations in the plasma of several mammals and is associated with the development of hypertension. In addition, plasma ouabain is increased in several hypertension models, and the acute or chronic administration of ouabain increases blood pressure in rodents. These results suggest a possible association between ouabain and the genesis or development and maintenance of arterial hypertension. One explanation for this association is that ouabain binds to the α-subunit of the Na+ pump, inhibiting its activity. Inhibition of this pump increases intracellular Na+, which reduces the activity of the sarcolemmal Na+/Ca2+ exchanger and thereby reduces Ca2+ extrusion. Consequently,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ouabain; Hypertension; Vascular reactivity; Renin-angiotensin system.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900015

Altered heart rate and blood pressure variability in mice lacking the Mas protooncogene
Provedor de dados: 56
Autores: Walther,T.; Wessel,N.; Kang,N.; Sander,A.; Tschöpe,C.; Malberg,H.; Bader,M.; Voss,A.
Heart rate variability is a relevant predictor of cardiovascular risk in humans. A significant genetic influence on heart rate variability is suggested, although the genes involved are ill-defined. The Mas-protooncogene encodes a G-protein-coupled receptor with seven transmembrane domains highly expressed in testis and brain. Since this receptor is supposed to interact with the signaling of angiotensin II, which is an important regulator of cardiovascular homeostasis, heart rate and blood pressure were analyzed in Mas-deficient mice. Using a femoral catheter the blood pressure of mice was measured for a period of 30 min and 250 data values per second were recorded. The mean values and range of heart rate and blood pressure were then calculated. Neither...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood pressure variability; Heart rate variability; Baroreflex sensitivity; Mas protooncogene.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100001

Angiotensin-II-induced reactive oxygen species along the SFO-PVN-RVLM pathway: implications in neurogenic hypertension
Provedor de dados: 56
Autores: Braga,V.A.; Medeiros,I.A.; Ribeiro,T.P.; França-Silva,M.S.; Botelho-Ono,M.S.; Guimarães,D.D.
Neurogenic hypertension has been the subject of extensive research worldwide. This review is based on the premise that some forms of neurogenic hypertension are caused in part by the formation of angiotensin-II (Ang-II)-induced reactive oxygen species along the subfornical organ-paraventricular nucleus of the hypothalamus-rostral ventrolateral medulla pathway (SFO-PVN-RVLM pathway). We will discuss the recent contribution of our laboratory and others regarding the mechanisms by which neurons in the SFO (an important circumventricular organ) are activated by Ang-II, how the SFO communicates with two other important areas involved in sympathetic activity regulation (PVN and RVLM) and how Ang-II-induced reactive oxygen species participate along the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide; Sympathetic; Angiotensin; Subfornical organ; Paraventricular nucleus of the hypothalamus; Rostral ventrolateral medulla.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900007

Effects of losartan combined with exercise training in spontaneously hypertensive rats
Provedor de dados: 56
Autores: Azevedo,L.F.; Brum,P.C.; Mattos,K.C.; Junqueira,C.M.; Rondon,M.U.P.B.; Barretto,A.C.P.; Negrao,C.E.
We investigate whether combined treatment with losartan, an angiotensin II receptor blocker, and exercise training (ET) in spontaneously hypertensive rats (SHR) would have an additive effect in reducing hypertension and improving baroreflex sensitivity when compared with losartan alone. Male SHR (8 weeks old) were assigned to 3 groups: sedentary placebo (SP, N = 16), sedentary under losartan treatment (SL, N = 11; 10 mg kg-1 day-1, by gavage), and ET under losartan treatment (TL, N = 10). ET was performed on a treadmill 5 days/week for 60 min at 50% of peak VO2, for 18 weeks. Blood pressure (BP) was measured with a catheter inserted into the carotid artery, and cardiac output with a microprobe placed around the ascending aorta. The baroreflex control of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spontaneously hypertensive rats; Exercise training; Losartan; Baroreflex sensitivity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100018

A study of the abilities in oral language comprehension of the Boston Diagnostic Aphasia Examination - Portuguese version: a reference guide for the Brazilian population
Provedor de dados: 56
Autores: Mansur,L.L.; Radanovic,M.; Taquemori,L.; Greco,L.; Araújo,G.C.
We analyzed the performance of 162 normal subjects, subdivided into groups according to age and schooling, in the oral comprehension tasks of the Boston Diagnostic Aphasia Examination translated and adapted to Brazilian Portuguese to obtain a profile of performance for the Brazilian population, as well as cut-off scores for each task, and to determine the best combination of tasks that distinguish normal from aphasic subjects, as a guide for clinicians. The normal subjects were compared to 69 aphasics. Age alone influenced the performance in the designation of actions (subjects above 70 years showing the worst performance); schooling alone influenced the comprehension of forms, colors and numbers (subjects with less than four years of education showing a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral comprehension; Age; Schooling; Boston Diagnostic Aphasia Examination; Brazilian Portuguese.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200017

A tribute to Samuel MacDonald McCann (1925-2007): in honor of one of the last pioneers of Neuroendocrinology
Provedor de dados: 56
Autores: Antunes-Rodrigues,J.; Bedran de Castro,J.C.; Franci,C.R.; Anselmo Franci,J.A.; Pazos Moura,C.C.; Moura,E.G.; Marubayashi,U.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500013

Classification of brain tumor extracts by high resolution ¹H MRS using partial least squares discriminant analysis
Provedor de dados: 56
Autores: Faria,A.V.; Macedo Jr.,F.C.; Marsaioli,A.J.; Ferreira,M.M.C.; Cendes,F.
High resolution proton nuclear magnetic resonance spectroscopy (¹H MRS) can be used to detect biochemical changes in vitro caused by distinct pathologies. It can reveal distinct metabolic profiles of brain tumors although the accurate analysis and classification of different spectra remains a challenge. In this study, the pattern recognition method partial least squares discriminant analysis (PLS-DA) was used to classify 11.7 T ¹H MRS spectra of brain tissue extracts from patients with brain tumors into four classes (high-grade neuroglial, low-grade neuroglial, non-neuroglial, and metastasis) and a group of control brain tissue. PLS-DA revealed 9 metabolites as the most important in group differentiation: γ-aminobutyric acid, acetoacetate, alanine,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Tumor; Magnetic resonance spectroscopy; Spectroscopy; Metabolism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200009

VPA/PLGA microfibers produced by coaxial electrospinning for the treatment of central nervous system injury
Provedor de dados: 56
Autores: Reis,K.P.; Sperling,L.E.; Teixeira,C.; Sommer,L.; Colombo,M.; Koester,L.S.; Pranke,P.
The central nervous system shows limited regenerative capacity after injury. Spinal cord injury (SCI) is a devastating traumatic injury resulting in loss of sensory, motor, and autonomic function distal from the level of injury. An appropriate combination of biomaterials and bioactive substances is currently thought to be a promising approach to treat this condition. Systemic administration of valproic acid (VPA) has been previously shown to promote functional recovery in animal models of SCI. In this study, VPA was encapsulated in poly(lactic-co-glycolic acid) (PLGA) microfibers by the coaxial electrospinning technique. Fibers showed continuous and cylindrical morphology, randomly oriented fibers, and compatible morphological and mechanical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coaxial electrospinning; VPA; Spinal cord injury.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400606

Swimming training increases cardiac vagal activity and induces cardiac hypertrophy in rats
Provedor de dados: 56
Autores: Medeiros,A.; Oliveira,E.M.; Gianolla,R.; Casarini,D.E.; Negrão,C.E.; Brum,P.C.
The effect of swimming training (ST) on vagal and sympathetic cardiac effects was investigated in sedentary (S, N = 12) and trained (T, N = 12) male Wistar rats (200-220 g). ST consisted of 60-min swimming sessions 5 days/week for 8 weeks, with a 5% body weight load attached to the tail. The effect of the autonomic nervous system in generating training-induced resting bradycardia (RB) was examined indirectly after cardiac muscarinic and adrenergic receptor blockade. Cardiac hypertrophy was evaluated by cardiac weight and myocyte morphometry. Plasma catecholamine concentrations and citrate synthase activity in soleus muscle were also determined in both groups. Resting heart rate was significantly reduced in T rats (355 ± 16 vs 330 ± 20 bpm). RB was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swimming-training exercise; Cardiac autonomic balance; Hemodynamics; Hypertrophic effect; Parasympathetic system; Resting bradycardia.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200018

Astragalus polysaccharides decrease proliferation, migration, and invasion but increase apoptosis of human osteosarcoma cells by up-regulation of microRNA-133a
Provedor de dados: 56
Autores: Chu,Yanchen; Fang,Yuan; Chi,Jingwei; Li,Jing; Zhang,Dongyang; Zou,Yunwen; Wang,Zhijie.
Osteosarcoma (OS) has a high incidence, malignity, and frequency of recurrence and metastasis. In this study, we aimed to explore the potential anti-cancer effects of Astragalus polysaccharides (APS) on human OS MG63 cells as well as underlying mechanisms. Viability of MG63 cells was assessed by CCK-8 assay to determine the adequate concentration of APS. Then, effects of APS on MG63 cell proliferation, cell cycle distribution, apoptosis, and migration and invasion were analyzed by BrdU incorporation, PI staining, flow cytometry, and transwell assays, respectively. The expression levels of proteins involved in these physiological processes were assessed by western blot analysis. Afterwards, miR-133a level in APS-treated cells was determined by qRT-PCR, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteosarcoma; Astragalus polysaccharides; Anti-tumor; MicroRNA-133a; JNK.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200610

Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons
Provedor de dados: 56
Autores: Santos,D.V.V.; Costa,K.M.; Vaz,M.C.G.; Silva Filho,M. Da.
The cortical layer 1 contains mainly small interneurons, which have traditionally been classified according to their axonal morphology. The dendritic morphology of these cells, however, has received little attention and remains ill defined. Very little is known about how the dendritic morphology and spatial distribution of these cells may relate to functional neuronal properties. We used biocytin labeling and whole cell patch clamp recordings, associated with digital reconstruction and quantitative morphological analysis, to assess correlations between dendritic morphology, spatial distribution and membrane properties of rat layer 1 neurons. A total of 106 cells were recorded, labeled and subjected to morphological analysis. Based on the quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biocytin; Patch-clamp; Dendritic morphology; Layer 1; Neuronal variability.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200016

In vitro cytotoxicity of the LDE: daunorubicin complex in acute myelogenous leukemia blast cells
Provedor de dados: 56
Autores: Dorlhiac-Llacer,P.E.; Marquezini,M.V.; Toffoletto,O.; Carneiro,R.C.G.; Maranhão,R.C.; Chamone,D.A.F.
Acute myelogenous leukemia (AML) blast cells show high-affinity degradation of low-density lipoprotein (LDL), suggesting an increased expression of cellular LDL receptors. LDE is a lipid microemulsion easily synthesized in vitro which is known to mimic the metabolic pathway of LDL. We used LDE as a carrier for daunorubicin and assayed the cytotoxicity of the complex using AML blast cells since RT-PCR analysis showed that AML cells express LDL receptor mRNA. The LDE:daunorubicin complex killed 46.7% of blast cells and 20.2% of normal bone marrow cells (P&lt;0.001; Student t-test). Moreover, this complex destroyed AML blast cells as efficiently as free daunorubicin. Thus, LDE might be a suitable carrier of chemotherapeutic agents targeting these drugs to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Acute myelogenous leukemia; Daunorubicin; Low-density lipoprotein; LDE complex; RT-PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000004

Efficacy of the FEV1/FEV6 ratio compared to the FEV1/FVC ratio for the diagnosis of airway obstruction in subjects aged 40 years or over
Provedor de dados: 56
Autores: Rosa,F.W.; Perez-Padilla,R.; Camelier,A.; Nascimento,O.A.; Menezes,A.M.B.; Jardim,J.R.
The present cross-sectional, population-based study was designed to evaluate the performance of the FEV1/FEV6 ratio for the detection of airway-obstructed subjects compared to the FEV1/FVC &lt;0.70 fixed ratio test, as well as the lower limit of normality (LLN) for 1000 subjects ³40 years of age in the metropolitan area of São Paulo, SP, Brazil. After the exclusion of 37 (3.7%) spirometries, a total of 963 pre-bronchodilator (BD) and 918 post-BD curves were constructed. The majority of the post-BD curves (93.1%) were of very good quality and achieved grade A (762 curves) or B (93 curves). The FEV1/FEV6 and FEV1/FVC ratios were highly correlated (r² = 0.92, P &lt; 0.000). Two receiver operator characteristic curves were constructed in order to express the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forced expiratory volume in six seconds; Airway obstruction; Diagnosis; Forced vital capacity; Pulmonary function tests; Spirometry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200004

Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals
Provedor de dados: 56
Autores: Barabás,É.; Nagy,K.; Várkonyi,V.; Horváth,A.
IFN-gamma mRNA expression was evaluated in nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT-PCR and the sensitivity of these methods was compared. A significant correlation was found between quantitative competitive and semiquantitative RT-PCR in samples of both HIV-seronegative (P = 0.004) and HIV-infected individuals (P = 0.0004). PBMC from HIV-infected individuals presented a remarkable increase of IFN-gamma mRNA expression, as determined by both types of RT-PCR methods. Semiquantitative RT-PCR even without an internal standard is also acceptable for measuring cytokine mRNA expression, but less reliable if small amounts are quantified. Moreover, we...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Quantitative competitive RT-PCR; Semiquantitative RT-PCR; IFN-gamma mRNA; HIV.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000006

Effect of electrolytic lesion of the dorsal raphe nucleus on water intake and sodium appetite
Provedor de dados: 56
Autores: Olivares,E.L.; Costa-e-Sousa,R.H.; Cavalcante-Lima,H.R.; Lima,H.R.C.; Cedraz-Mercez,P.L.; Reis,L.C.
The present study determined the effect of an electrolytic lesion of the dorsal raphe nucleus (DRN) on water intake and sodium appetite. Male Wistar rats weighing 290-320 g with a lesion of the DRN (L-DRN), performed two days before experiments and confirmed by histology at the end of the experiments, presented increased sensitivity to the dehydration induced by fluid deprivation. The cumulative water intake of L-DRN rats reached 23.3 ± 1.9 ml (a 79% increase, N = 9) while sham-lesioned rats (SL-DRN) did not exceed 13.0 ± 1.0 ml (N = 11, P &lt; 0.0001) after 5 h. The L-DRN rats treated with isoproterenol (300 µg kg-1 ml-1, sc) exhibited an increase in water intake that persisted throughout the experimental period (L-DRN, 15.7 ± 1.47 ml, N = 9 vs SL-DRN,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sodium appetite; Water intake; Dorsal raphe nucleus; Serotonergic system; Electrolytic lesion; Rats.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200013

Serum and cerebrospinal fluid concentrations of E-selectin in patients with aneurysmal subarachnoid hemorrhage
Provedor de dados: 56
Autores: Tanriverdi,T.; Sanus,G.Z.; Ulu,M.O.; Tureci,E.; Uzun,H.; Aydin,S.; Kaynar,M.Y.
The goal of the present study was to determine concentrations of E-selectin in both cerebrospinal fluid (CSF) and serum of patients with aneurysmal subarachnoid hemorrhage (SAH) and to evaluate the correlation between the clinical parameters and E-selectin levels. Both CSF and serum samples obtained from 12 patients with aneurysmal SAH and 8 patients with hydrocephalus (control group) without any other known central nervous system disease were assayed for E-selectin by quantitative enzyme-linked immunosorbent assay and the results were compared between the two groups. Mean levels of soluble forms of E-selectin within the first 3 days and on the 5th and 7th days of SAH were 4.0 ± 7.9, 2.8 ± 5.2, and 3.1 ± 4.9 ng/ml in the patient's CSF, and 33.7 ± 9.2, 35.1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion molecules; E-selectin; Selectin; Subarachnoid hemorrhage; Vasospasm.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100020

Influence of the neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos
Provedor de dados: 56
Autores: Alves,H.J.; Alvares,L.E.; Gabriel,J.E.; Coutinho,L.L.
Important advances have been made in understanding the genetic processes that control skeletal muscle formation. Studies conducted on quails detected a delay in the myogenic program of animals selected for high growth rates. These studies have led to the hypothesis that a delay in myogenesis would allow somitic cells to proliferate longer and consequently increase the number of embryonic myoblasts. To test this hypothesis, recently segmented somites and part of the unsegmented paraxial mesoderm were separated from the neural tube/notochord complex in HH12 chicken embryos. In situ hybridization and competitive RT-PCR revealed that MyoD transcripts, which are responsible for myoblast determination, were absent in somites separated from neural tube/notochord...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken development; MyoD; Myogenesis; In situ hybridization; BrdU; Cellular proliferation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200005

14-3-3 proteins in apoptosis
Provedor de dados: 56
Autores: Rosenquist,M.
The once obscure members of the 14-3-3 protein family play significant roles in the determination of cell fate. By inhibiting the pro-apoptotic BAD (Bcl-2-antagonist of cell death) and the transcription factor FKHRL-1, 14-3-3 displays important anti-apoptotic characteristics. To date, five points of interaction of 14-3-3 with the apoptotic machinery have been identified. How these interactions are regulated still remains a mystery.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 14-3-3; Apoptosis; Programmed cell death.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400001

Association of Alport's syndrome with HLA-DR2 antigen in a group of unrelated patients
Provedor de dados: 56
Autores: Donadi,E.A.; Voltarelli,J.C.; Paula-Santos,C.M.; Kimachi,T.; Ferraz,A.S.
A few family studies have evaluated HLA antigens in Alport's syndrome; however, there are no large population studies. In the present report, we studied 40 unrelated white patients with Alport's syndrome seen at the Unit of Renal Transplantation, Faculty of Medicine of Ribeirão Preto, São Paulo, Brazil. HLA-A, -B, -DR and -DQ antigens were typed using a complement-dependent microlymphocytotoxicity assay. A control white population (N = 403) from the same geographical area was also typed for HLA antigens. Although the frequencies of HLA-A and -B antigens of patients were not statistically different from controls, the frequency of HLA-DR2 antigen observed in patients (65%) was significantly increased in relation to controls (26%; P&amp;lt;0.001). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA antigens; Alport's syndrome; MHC; Immunogenetics.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400010

Protective effects of tumor necrosis factor-α blockade by adalimumab on articular cartilage and subchondral bone in a rat model of osteoarthritis
Provedor de dados: 56
Autores: Ma,C.H.; Lv,Q.; Yu,Y.X.; Zhang,Y.; Kong,D.; Niu,K.R.; Yi,C.Q.
We aimed to investigate the effects of an anti-tumor necrosis factor-α antibody (ATNF) on cartilage and subchondral bone in a rat model of osteoarthritis. Twenty-four rats were randomly divided into three groups: sham-operated group (n=8); anterior cruciate ligament transection (ACLT)+normal saline (NS) group (n=8); and ACLT+ATNF group (n=8). The rats in the ACLT+ATNF group received subcutaneous injections of ATNF (20 μg/kg) for 12 weeks, while those in the ACLT+NS group received NS at the same dose for 12 weeks. All rats were euthanized at 12 weeks after surgery and specimens from the affected knees were harvested. Hematoxylin and eosin staining, Masson's trichrome staining, and Mankin score assessment were carried out to evaluate the cartilage status and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-tumor necrosis factor-α antibody (ATNF); Osteoarthrosis; Matrix metalloproteinase (MMP)-13; Articular cartilage; Subchondral bone.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000001

Biomechanical and histological evaluation of hydrogel implants in articular cartilage
Provedor de dados: 56
Autores: Malmonge,S.M.; Zavaglia,C.A.C.; Belangero,W.D.
We evaluated the mechanical behavior of the repaired surfaces of defective articular cartilage in the intercondylar region of the rat femur after a hydrogel graft implant. The results were compared to those for the adjacent normal articular cartilage and for control surfaces where the defects remained empty. Hydrogel synthesized by blending poly(2-hydroxyethyl methacrylate) and poly(methyl methacrylate-co-acrylic acid) was implanted in male Wistar rats. The animals were divided into five groups with postoperative follow-up periods of 3, 5, 8, 12 and 16 weeks. Indentation tests were performed on the neoformed surfaces in the knee joint (with or without a hydrogel implant) and on adjacent articular cartilage in order to assess the mechanical properties of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomaterial; Cartilage repair; Hydrogel; Biomechanical evaluation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300008

Analysis of the diagnostic presentation profile, parathyroidectomy indication and bone mineral density follow-up of Brazilian patients with primary hyperparathyroidism
Provedor de dados: 56
Autores: Oliveira,U.E.M.; Ohe,M.N.; Santos,R.O.; Cervantes,O.; Abrahão,M.; Lazaretti-Castro,M.; Vieira,J.G.H.; Hauache,O.M.
Primary hyperparathyroidism is an endocrine disorder with variable clinical expression, frequently presenting as asymptomatic hypercalcemia in Western countries but still predominantly as a symptomatic disease in developing countries. The objective of this retrospective study was to describe the diagnostic presentation profile, parathyroidectomy indication and post-surgical bone mineral density follow-up of patients with primary hyperparathyroidism seen at a university hospital. We found 115 patients (92 women, median age 56 years) with primary hyperparathyroidism diagnosed during the last 20 years. We defined symptomatic patients based on the presence of any classical symptom affecting bone, kidney or the neuromuscular system. Surgical criteria followed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primary hyperparathyroidism; Hypercalcemia; Clinical presentation; Parathyroidectomy; Bone mineral density.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400010

Familial predisposition to hypertension and the association between urinary sodium excretion and blood pressure in a population-based sample of young adults
Provedor de dados: 56
Autores: Moraes,R.S.; Fuchs,F.D.; Dalla Costa,F.; Moreira,L.B.
The reasons for the inconsistent association between salt consumption and blood pressure levels observed in within-society surveys are not known. A total of 157 normotensive subjects aged 18 to 35 years, selected at random in a cross-sectional population-based survey, answered a structured questionnaire. They were classified as strongly predisposed to hypertension when two or more first-degree relatives had a diagnosis of hypertension. Anthropometric parameters were obtained and sitting blood pressure was determined with aneroid sphygmomanometers. Sodium and potassium excretion was measured by flame spectrophotometry in an overnight urine sample. A positive correlation between blood pressure and urinary sodium excretion was detected only in the group of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Familial predisposition to hypertension; Urinary sodium excretion; Salt sensitivity.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700010

Clustering of psychiatric and somatic illnesses in the general population: multimorbidity and socioeconomic correlates
Provedor de dados: 56
Autores: Andrade,L.H.; Benseñor,I.M.; Viana,M.C.; Andreoni,S.; Wang,Y.-P.
The distribution of psychiatric disorders and of chronic medical illnesses was studied in a population-based sample to determine whether these conditions co-occur in the same individual. A representative sample (N = 1464) of adults living in households was assessed by the Composite International Diagnostic Interview, version 1.1, as part of the São Paulo Epidemiological Catchment Area Study. The association of sociodemographic variables and psychological symptoms regarding medical illness multimorbidity (8 lifetime somatic conditions) and psychiatric multimorbidity (15 lifetime psychiatric disorders) was determined by negative binomial regression. A total of 1785 chronic medical conditions and 1163 psychiatric conditions were detected in the population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comorbidity; Multimorbidity coefficient; Negative binomial regression; Common psychological symptoms; Socioeconomic status; Chronic medical illness.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500010

lncRNA ZEB1-AS1 inhibits high glucose-induced EMT and fibrogenesis by regulating the miR-216a-5p/BMP7 axis in diabetic nephropathy
Provedor de dados: 56
Autores: Meng,Qingqing; Zhai,Xiaolin; Yuan,Yi; Ji,Qing; Zhang,Pengyuan.
Diabetic nephropathy (DN) is one of the leading causes of mortality in diabetic patients. Long non-coding RNA zinc finger E-box binding homeobox 1 antisense 1 (ZEB1-AS1) plays a crucial role in the development of various diseases, including DN. However, the molecular mechanism of ZEB1-AS1 in DN pathogenesis remains elusive. An in vitro DN model was established by treating HK-2 cells with high glucose (HG). Quantitative polymerase chain reaction (qRT-PCR) was utilized to detect the expression levels of ZEB1-AS1, microRNA-216a-5p (miR-216a-5p), and bone morphogenetic protein 7 (BMP7). Western blot assay was used to evaluate the protein levels of BMP7, epithelial-to-mesenchymal transition (EMT)-related proteins, and fibrosis markers. Additionally, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ZEB1-AS1; MiR-216a-5p; BMP7; Diabetic nephropathy; EMT; Fibrogenesis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400607

Mechanisms in anti-inflammation and resolution: the role of lipoxins and aspirin-triggered lipoxins
Provedor de dados: 56
Autores: Fierro,I.M.; Serhan,C.N.
Multicellular host responses to infection, injury or inflammatory stimuli lead to the formation of a broad range of chemical mediators by the host. The integrated response of the host is essential to health and disease; thus it is important to achieve a more complete understanding of the molecular and cellular events governing the formation and actions of endogenous mediators of resolution that appear to control the duration of inflammation. Lipoxins are trihydroxytetraene-containing lipid mediators that can be formed during cell-cell interactions and are predominantly counterregulators of some well-known mediators of inflammation. Since this circuit of lipoxin formation and action appears to be of physiological relevance for the resolution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Lipoxins; Resolution.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500002

Spirometry reference values in the Brazilian population
Provedor de dados: 56
Autores: Rufino,R.; Costa,C.H.; Lopes,A.J.; Maiworm,A.I.; Maynard,K.; Silva,L.M.R.A.; Dias,R.M.
The aim of the present study was to provide new spirometry reference equations in a sample of the Brazilian population for the following parameters: forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), FEV1/FVC ratio, peak of expiratory flow (PEF), forced expiratory flow at 50% (FEF50%), 75% average vital capacity (FEF25-75%), and average forced expiratory flow time (FEFT). This was a prospective study using results from chest radiographs, electrocardiograms, and questionnaires to investigate the participants' respiratory symptoms, sedentarism, and comorbidities (Charlson comorbidity index). From December 2010 to July 2014, individuals were randomly selected from various locations in the state of Rio de Janeiro. All individuals were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spirometry; Lung function test; Reference values; FVC; FEV1.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300703

Racial inequalities and perinatal health in the southeast region of Brazil
Provedor de dados: 56
Autores: Silva,L.M.; Silva,R.A.; Silva,A.A.M.; Bettiol,H.; Barbieri,M.A.
Few studies are available about racial inequalities in perinatal health in Brazil and little is known about whether the existing inequality is due to socioeconomic factors or to racial discrimination per se. Data regarding the Ribeirão Preto birth cohort, Brazil, whose mothers were interviewed from June 1, 1978 to May 31, 1979 were used to answer these questions. The perinatal factors were obtained from the birth questionnaire and the ethnic data were obtained from 2063 participants asked about self-reported skin color at early adulthood (23-25 years of age) in 2002/2004. Mothers of mulatto and black children had higher rates of low schooling (£4 years, 27.2 and 38.0%) and lower family income (£1 minimum wage, 28.6 and 30.4%). Mothers aged less than 20...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethnic-racial discrimination; Prenatal care; Preterm birth; Low birth weight; Perinatal health; Cesarean section.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900005

Distribution of versican and hyaluronan in the mouse uterus during decidualization
Provedor de dados: 56
Autores: San Martin,S.; Soto-Suazo,M.; Zorn,T.M.T.
Preparation for embryo implantation requires extensive adaptation of the uterine microenvironment. This process consists of cell proliferation and cell differentiation resulting in the transformation of endometrial fibroblasts into a new type of cell called decidual cell. In the present study, we followed the space-time distribution of versican and hyaluronan (HA) in different tissues of the uterus before and after embryo implantation. Fragments of mouse uteri obtained on the fourth, fifth, sixth and seventh days of pregnancy were fixed in Methacarn, embedded in Paraplast and cut into 5-µm thick sections. HA was detected using a biotinylated fragment of the proteoglycan aggrecan, which binds to this glycosaminoglycan with high affinity and specificity....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decidualization; Endometrium; Proteoglycans; Versican; Hyaluronan; Mouse.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800013

Regulation of the renal proximal tubule second sodium pump by angiotensins
Provedor de dados: 56
Autores: Caruso-Neves,C.; Rangel,L.B.A.; Lara,L.S.; Lopes,A.G.
For several years it was believed that angiotensin II (Ang II) alone mediated the effects of the renin-angiotensin system. However, it has been observed that other peptides of this system, such as angiotensin-(1-7) (Ang-(1-7)), present biological activity. The effect of Ang II and Ang-(1-7) on renal sodium excretion has been associated, at least in part, with modulation of proximal tubule sodium reabsorption. In the present review, we discuss the evidence for the involvement of Na+-ATPase, called the second sodium pump, as a target for the actions of these compounds in the regulation of proximal tubule sodium reabsorption.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Na+-ATPase; Angiotensin II; Angiotensin-(1-7); Furosemide; Proximal tubule.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800015

Chronic kidney disease in disadvantaged populations
Provedor de dados: 56
Autores: Garcia-Garcia,G.; Jha,V.
The increased burden of chronic kidney disease (CKD) in disadvantaged populations is due to both global factors and population-specific issues. Low socioeconomic status and poor access to care contribute to health care disparities and exacerbate the negative effects of genetic or biological predisposition. Provision of appropriate renal care to these populations requires a two-pronged approach: expanding the reach of dialysis through development of low-cost alternatives that can be practiced in remote locations, and implementation and evaluation of cost-effective prevention strategies. Kidney transplantation should be promoted by expansion of deceased donor transplant programs and use of inexpensive, generic immunosuppressive drugs. The message of World...
Tipo: Info:eu-repo/semantics/article
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500377

The expression of the ACTH receptor
Provedor de dados: 56
Autores: Elias,L.L.K.; Clark,A.J.L.
Adrenal glucocorticoid secretion is regulated by adrenocorticotropic hormone (ACTH) acting through a specific cell membrane receptor (ACTH-R). The ACTH-R is a member of the G protein superfamily-coupled receptors and belongs to the subfamily of melanocortin receptors. The ACTH-R is mainly expressed in the adrenocortical cells showing a restricted tissue specificity, although ACTH is recognized by the other four melanocortin receptors. The cloning of the ACTH-R was followed by the study of this gene in human diseases such as familial glucocorticoid deficiency (FGD) and adrenocortical tumors. FGD is a rare autosomal recessive disease characterized by glucocorticoid deficiency, elevated plasma ACTH levels and preserved renin/aldosterone secretion. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACTH receptor; Resistance; Mutation; Transfection.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000015

Method for automatic detection of wheezing in lung sounds
Provedor de dados: 56
Autores: Riella,R.J.; Nohama,P.; Maia,J.M.
The present report describes the development of a technique for automatic wheezing recognition in digitally recorded lung sounds. This method is based on the extraction and processing of spectral information from the respiratory cycle and the use of these data for user feedback and automatic recognition. The respiratory cycle is first pre-processed, in order to normalize its spectral information, and its spectrogram is then computed. After this procedure, the spectrogram image is processed by a two-dimensional convolution filter and a half-threshold in order to increase the contrast and isolate its highest amplitude components, respectively. Thus, in order to generate more compressed data to automatic recognition, the spectral projection from the processed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheezes; Lung sounds; Spectrogram; Digital image processing; Artificial neural networks.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700013

Effect of high hydrostatic pressure on the barrier properties of polyamide-6 films
Provedor de dados: 56
Autores: Schmerder,A.; Richter,T.; Langowski,H.-C.; Ludwig,H.
Little is known about the barrier properties of polymer films during high pressure processing of prepackaged foods. In order to learn more about this, we examined the influence of high hydrostatic pressure on the permeation of raspberry ketone (dissolved in ethanol/water) through polyamide-6 films at temperatures between 20 and 60ºC. Permeation was lowered by increasing pressure at all temperatures. At 23°C, the increasing pressure sequence 0.1, 50, 100, 150, and 200 MPa correlated with the decreasing permeation coefficients P/(10(9) cm² s-1) of 6.2, 3.8, 3.0, 2.2, and 1.6. Analysis of the permeation kinetics indicated that this effect was due to a reduced diffusion coefficient. Pressure and temperature acted antagonistically to each other. The decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrostatic pressure; Polyamide-6 films; Permeation through polymers; Raspberry ketone.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800018

Sentinel node biopsy using blue dye and technetium99 in advanced gastric cancer: anatomical drainage and clinical application
Provedor de dados: 56
Autores: Santos,F.A.V.; Drummond-Lage,A.P.; Rodrigues,M.A.; Cabral,M.A.; Pedrosa,M.S.; Braga,H.; Wainstein,A.J.A.
Lymph node metastases are an independent prognosis factor in gastric carcinoma (GC) patients. Radical lymphadenectomy can improve survival but it can also increase surgical morbidity. As a principle, sentinel node (SN) navigation surgery can avoid unnecessary lymphadenectomy without compromising prognosis. In this pilot study, 24 patients with untreated GC were initially screened for SN navigation surgery, of which 12 were eligible. Five patients had T2 tumors, 5 had T3 tumors and 2 had T1 tumors. In 33% of cases, tumor diameter was greater than 5.0 cm. Three hundred and eighty-seven lymph nodes were excised with a median of 32.3 per patient. The SN navigation surgery was feasible in all patients, with a median of 4.5 SNs per patient. The detection success...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric carcinoma; Sentinel lymph node; Biopsy; Stage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800701

[RETRACTED ARTICLE] Ginsenoside Rd inhibits IL-1β-induced inflammation and degradation of intervertebral disc chondrocytes by increasing IL1RAP ubiquitination
Provedor de dados: 56
Autores: Zhang,Wei-Jia; Liu,Ying; Wei,Jie-Shu; Wu,Ya-Li.
Many compounds of ginsenosides show anti-inflammatory properties. However, their anti-inflammatory effects in intervertebral chondrocytes in the presence of inflammatory factors have never been shown. Increased levels of pro-inflammatory cytokines are generally associated with the degradation and death of chondrocytes; therefore, finding an effective and nontoxic substance that attenuates the inflammation is worthwhile. In this study, chondrocytes were isolated from the nucleus pulposus tissues, and the cells were treated with ginsenoside compounds and IL-1β, alone and in combination. Cell viability and death rate were assessed by CCK-8 and flow cytometry methods, respectively. PCR, western blot, and immunoprecipitation assays were performed to determine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginsenoside Rd; IL-1β; Inflammation; Degradation; Intervertebral disc chondrocytes; IL1RAP ubiquitination.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900602

Influence of schooling and age on cognitive performance in healthy older adults
Provedor de dados: 56
Autores: Bento-Torres,N.V.O.; Bento-Torres,J.; Tomás,A.M.; Costa,V.O.; Corrêa,P.G.R.; Costa,C.N.M.; Jardim,N.Y.V.; Picanço-Diniz,C.W.
Few studies have examined the influence of a low level of schooling on age-related cognitive decline in countries with wide social and economic inequalities by using the Cambridge Automated Neuropsychological Test Battery (CANTAB). The aim of the present study was to assess the influence of schooling on age-related cognitive decline using unbiased cognitive tests. CANTAB allows cognitive assessment across cultures and education levels with reduced interference of the examiner during data acquisition. Using two-way ANOVA, we assessed the influences of age and education on test scores of old adults (61–84 years of age). CANTAB tests included: Visual Sustained Attention, Reaction Time, Spatial Working Memory, Learning and Episodic Memory. All subjects had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age-related cognitive decline; Primary prevention; Education; Neuropsychological tests; Memory; Neuroscience; CANTAB.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400702

Correlations between ANP concentrations in atria, plasma and cerebral structures and sodium chloride preference in Wistar rats
Provedor de dados: 56
We determined whether ANP (atrial natriuretic peptide) concentrations, measured by radioimmunoassay, in the ANPergic cerebral regions involved in regulation of sodium intake and excretion and pituitary gland correlated with differences in sodium preference among 40 Wistar male rats (180-220 g). Sodium preference was measured as mean spontaneous ingestion of 1.5% NaCl solution during a test period of 12 days. The relevant tissues included the olfactory bulb (OB), the posterior and anterior lobes of the pituitary gland (PP and AP, respectively), the median eminence (ME), the medial basal hypothalamus (MBH), and the region anteroventral to the third ventricle (AV3V). We also measured ANP content in the right (RA) and left atrium (LA) and plasma. The...
Palavras-chave: Sodium chloride intake; Sodium chloride preference; Atrial natriuretic peptide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100010

Lymphatic fluctuation in the parenchymal remodeling stage of acute interstitial pneumonia, organizing pneumonia, nonspecific interstitial pneumonia and idiopathic pulmonary fibrosis
Provedor de dados: 56
Autores: Parra,E.R.; Araujo,C.A.L.; Lombardi,J.G.; Ab’Saber,A.M.; Carvalho,C.R.R.; Kairalla,R.A.; Capelozzi,V.L.
Because the superficial lymphatics in the lungs are distributed in the subpleural, interlobular and peribroncovascular interstitium, lymphatic impairment may occur in the lungs of patients with idiopathic interstitial pneumonias (IIPs) and increase their severity. We investigated the distribution of lymphatics in different remodeling stages of IIPs by immunohistochemistry using the D2-40 antibody. Pulmonary tissue was obtained from 69 patients with acute interstitial pneumonia/diffuse alveolar damage (AIP/DAD, N = 24), cryptogenic organizing pneumonia/organizing pneumonia (COP/OP, N = 6), nonspecific interstitial pneumonia (NSIP/NSIP, N = 20), and idiopathic pulmonary fibrosis/usual interstitial pneumonia (IPF/UIP, N = 19). D2-40+ lymphatic in the lesions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphangiogenesis; Lymphatic vessel; Idiopathic interstitial pneumonias; Lung remodeling; Immunohistochemistry; Morphometry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500013

Identification of IgE and IgG1 specific antigens in Echinococcus granulosus cyst fluid
Provedor de dados: 56
Autores: Li,S.; Qian,R.; Wang,S.; Ye,J.; Zheng,H.
Cystic echinococcosis (CE) is an anthropozoonotic disease with worldwide distribution and is caused by the cestode Echinococcus granulosus. Anaphylactic shock induced by CE rupture is a serious complication especially in patients with hydatid infections, as the resulting leakage of fluid contains highly toxic endogenous antigen. We aimed to isolate and identify the antigens of specific IgE and IgG1 (sIgE and sIgG1) in E. granulosus cyst fluid (EgCF). Crude antigen for EgCF was prepared from E. granulosus-infected sheep liver. Antigens were separated and identified by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE), two-dimensional gel electrophoresis (2-DE), and immunoblotting. Results of 1D SDS-PAGE and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic echinococcosis; Anaphylactic shock; Specific antigens; Specific antibody.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700701

Evaluation of hemostatic changes using thromboelastography after crystalloid or colloid fluid administration during major orthopedic surgery
Provedor de dados: 56
Autores: Topçu,I.; Çivi,M.; Öztürk,T.; Keleş,G.T.; Çoban,S.; Yentür,E.A.; Okçu,G.
The effects of Ringer lactate, 6% hydroxyethyl starch (HES) (130/0.4) or 4% succinylated gelatin solutions on perioperative coagulability were measured by thromboelastography (TEG). Seventy-five patients (ASA I-III) who were to undergo major orthopedic procedures performed under epidural anesthesia were included in the study. Patients were randomly divided into three groups of 25 each for the administration of maintenance fluids: group RL (Ringer lactate), group HES (6% HES 130/0.4), and group JEL (4% gelofusine solution). Blood samples were obtained during the perioperative period before epidural anesthesia (t1, baseline), at the end of the surgery (t2), and 24 h after the operation (t3). TEG data, reaction time (R), coagulation time (K), angle value (α),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxyethyl starch; Ringer lactate; Gelatin; Coagulation; Thromboelastography; Orthopedic surgery.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900012

Influence of the dopaminergic system, CREB, and transcription factor-κB on cocaine neurotoxicity
Provedor de dados: 56
Autores: Planeta,C.S.; Lepsch,L.B.; Alves,R.; Scavone,C.
Cocaine is a widely used drug and its abuse is associated with physical, psychiatric and social problems. Abnormalities in newborns have been demonstrated to be due to the toxic effects of cocaine during fetal development. The mechanism by which cocaine causes neurological damage is complex and involves interactions of the drug with several neurotransmitter systems, such as the increase of extracellular levels of dopamine and free radicals, and modulation of transcription factors. The aim of this review was to evaluate the importance of the dopaminergic system and the participation of inflammatory signaling in cocaine neurotoxicity. Our study showed that cocaine activates the transcription factors NF-κB and CREB, which regulate genes involved in cellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocaine; Apoptosis; NF-κB; CREB; BDNF; Neurotoxicity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100909

The effects of surgery on gastrointestinal motor activity
Provedor de dados: 56
Autores: Quigley,.M.M.; Thompson,J.S.
Gastrointestinal surgical procedures have the potential to disrupt motor activity in various organs of the gastrointestinal tract or, indeed, throughout the entire alimentary canal. Several of these motor effects have important clinical consequences and have also served to advance our understanding of the regulation of gastrointestinal motor activity. This review will focus, in particular, on the effects of surgery on the small intestine, and will attempt to emphasize the implications of these studies for our understanding of small intestinal motility, in general.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Motility; Motor activity; Small intestine; Surgery; Intestinal resection; Intestinal transplantation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700003

Role of immunoglobulin E and mast cells in murine models of asthma
Provedor de dados: 56
Autores: Mayr,S.I.; Zuberi,R.I.; Liu,F.-T.
Immunoglobulin E (IgE) and mast cells are believed to play important roles in allergic inflammation. However, their contributions to the pathogenesis of human asthma have not been clearly established. Significant progress has been made recently in our understanding of airway inflammation and airway hyperresponsiveness through studies of murine models of asthma and genetically engineered mice. Some of the studies have provided significant insights into the role of IgE and mast cells in the allergic airway response. In these models mice are immunized systemically with soluble protein antigens and then receive an antigen challenge through the airways. Bronchoalveolar lavage fluid from mice with allergic airway inflammation contains significant amounts of IgE....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; IgE; Mast cells; Airway hyperresponsiveness.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700001

Influence of the test situation on pup retrieval behavior of normal and undernourished lactating rats
Provedor de dados: 56
Autores: Rocha,J.B.T.; Soares,F.A.A.; de Mello,C.F.
Undernutrition of dams and pups disrupts the retrieval efficiency of mothers. However, if the mothers are assessed in their home cages, they spend more time with their litters. In the present study the effect of test conditions on pup retrieval behavior of mothers receiving a 25% (well-nourished group) and 8% casein diet (undernourished group) was examined. In agreement with previous studies, undernourished mothers spent more time with their litters than well-nourished dams as lactation proceeded. Pup retrieval behavior varied with test conditions. In the first experiment, the maternal behavior of dams was assessed by the standard procedure (pups were separated from their mother and scattered over the floor of the home cage). The mother was then returned...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Retrieval; Undernourishment; Pup behavior; Lactating rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100013

Coping with genetic diversity: the contribution of pathogen and human genomics to modern vaccinology
Provedor de dados: 56
Autores: Lemaire,D.; Barbosa,T.; Rihet,P.
Vaccine development faces major difficulties partly because of genetic variation in both infectious organisms and humans. This causes antigenic variation in infectious agents and a high interindividual variability in the human response to the vaccine. The exponential growth of genome sequence information has induced a shift from conventional culture-based to genome-based vaccinology, and allows the tackling of challenges in vaccine development due to pathogen genetic variability. Additionally, recent advances in immunogenetics and genomics should help in the understanding of the influence of genetic factors on the interindividual and interpopulation variations in immune responses to vaccines, and could be useful for developing new vaccine strategies....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human vaccines; Vaccinomics; Genetic variation; Immunization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500001

Properties of a constitutive alkaline phosphatase from strain 74A of the mold Neurospora crassa
Provedor de dados: 56
Autores: Morales,A.C.; Nozawa,S.R.; Thedei Jr.,G.; Maccheroni Jr.,W.; Rossi,A.
A constitutive alkaline phosphatase was purified to apparent homogeneity as determined by polyacrylamide gel electrophoresis from mycelia of the wild strain 74A of the mold Neurospora crassa, after growth on acetate and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 h at 30ºC. The molecular mass was 58 kDa and 56 kDa as determined by exclusion chromatography and SDS-PAGE, respectively. This monomeric enzyme shows an apparent optimum pH ranging from 9.5 to 10.5 and Michaelis kinetics for the hydrolysis of p-nitrophenyl phosphate (the Km and Hill coefficient values were 0.35 mM and 1.01, respectively), alpha-naphthyl phosphate (the Km and Hill coefficient values were 0.44 mM and 0.97, respectively), ß-glycerol phosphate (the Km and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurospora crassa; Fungi; Alkaline phosphatase; L-histidinol-Pi phosphatase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800006

Unsteady walking as a symptom in type 2 diabetes mellitus: independent association with depression and sedentary lifestyle and no association with diabetic neuropathy
Provedor de dados: 56
Autores: Dias,L.S.; Nienov,O.H.; Goelzer Neto,C.F.; Schmid,H.
The purpose of this study was to look at the determinants of the unsteady walking (UW) symptom in patients with type 2 diabetes mellitus (T2DM) by defining if UW and/or the Diabetic Neuropathy Symptoms Score (DNSS) are associated with positive scores in Beck's Depression Inventory (BDI) and with a positive Michigan Neuropathy Screening Instrument score (MNSI). We evaluated 203 T2DM patients without visible gait disturbances. They were divided into UW (+) and UW (−) or DNSS (+) and DNSS (−) according to symptoms. We found a prevalence of 48.3% for UW (+) and of 63% for DNSS (+) in our sample. In univariate analysis, the presence of UW was significantly associated with waist circumference (P=0.024), number of comorbidities (P=0.012), not practicing physical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetic neuropathy; Depression; Unsteady walking; Lifestyle.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500613

Diabetic retinopathy is associated with early autonomic dysfunction assessed by exercise-related heart rate changes
Provedor de dados: 56
Autores: Kramer,C.K.; Leitão,C.B.; Azevedo,M.J.; Valiatti,F.B.; Rodrigues,T.C.; Canani,L.H.; Gross,J.L.
Diabetic retinopathy has been associated with cardiac autonomic dysfunction in both type 1 and type 2 diabetes mellitus (DM) patients. Heart rate (HR) changes during exercise testing indicate early alterations in autonomous tonus. The aim of the present study was to investigate the association of diabetic retinopathy with exercise-related HR changes. A cross-sectional study was performed on 72 type 2 and 40 type 1 DM patients. Autonomic dysfunction was assessed by exercise-related HR changes (Bruce protocol). The maximum HR increase, defined as the difference between the peak exercise rate and the resting rate at baseline, and HR recovery, defined as the reduction in HR from the peak exercise to the HR at 1, 2, and 4 min after the cessation of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Type 1 diabetes mellitus; Heart rate changes; Diabetic retinopathy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200011

Impact of congenital calcitonin deficiency due to dysgenetic hypothyroidism on bone mineral density
Provedor de dados: 56
Autores: Daripa,M.; Paula,F.J.A.; Rufino,A.C.B.; Foss,M.C.
The objective of the present study was to determine the effect of chronic calcitonin deficiency on bone mass development. The results of 11 patients with thyroid dysgenesis (TD) were compared to those of 17 normal individuals (C) and of 9 patients with other forms of hypothyroidism (OH): 4 with hypothyroidism due to inborn errors of thyroid hormone synthesis and 5 with Hashimoto's thyroiditis. The subjects received an intravenous calcium stimulus and blood was collected for the determination of ionized calcium (Ca2+), calcitonin, and intact parathyroid hormone. Bone mineral density (BMD) was determined by dual-energy X-ray absorptiometry. After calcium administration the levels of Ca2+ in the two groups of hypothyroidism were significantly higher than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Hypothyroidism; Calcitonin; Bone mineral density.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100009

Differential regulation of vitamin D receptor expression in distinct leukemic cell lines upon phorbol ester-induced growth arrest
Provedor de dados: 56
Autores: Folgueira,M.A.A.K.; Federico,M.H.H.; Roela,R.A.; Maistro,S.; Katayama,M.L.H.; Brentani,M.M.
A close correlation between vitamin D receptor (VDR) abundance and cell proliferation rate has been shown in NIH-3T3 fibroblasts, MCF-7 breast cancer and in HL-60 myeloblastic cells. We have now determined if this association occurs in other leukemic cell lines, U937 and K562, and if VDR content is related to c-myc expression, which is also linked to cell growth state. Upon phorbol myristate acetate (PMA) treatment, cells from the three lineages (HL-60, U937 and K562) differentiated and expressed specific surface antigens. All cell lines analyzed were growth inhibited by PMA and the doubling time was increased, mainly due to an increased fraction of cells in the G0/G1 phase, as determined by flow cytometry measurements of incorporated bromodeoxyuridine and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Receptors; Calcitriol; Cell division; Cell differentiation; Leukemia; Phorbol esters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500011

Impaired regeneration of dystrophin-deficient muscle fibers is caused by exhaustion of myogenic cells
Provedor de dados: 56
Autores: Luz,M.A.M.; Marques,M.J.; Santo Neto,H.
Duchenne muscular dystrophy is one of the most devastating myopathies. Muscle fibers undergo necrosis and lose their ability to regenerate, and this may be related to increased interstitial fibrosis or the exhaustion of satellite cells. In this study, we used mdx mice, an animal model of Duchenne muscular dystrophy, to assess whether muscle fibers lose their ability to regenerate after repeated cycles of degeneration-regeneration and to establish the role of interstitial fibrosis or exhaustion of satellite cells in this process. Repeated degenerative-regenerative cycles were induced by the injection of bupivacaine (33 mg/kg), a myotoxic agent. Bupivacaine was injected weekly into the right tibialis anterior muscle of male, 8-week-old mdx (N = 20) and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mdx fiber regeneration; Bupivacaine; Duchenne muscular dystrophy; Muscle degeneration.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600009

In vitro studies of multiwalled carbon nanotube/ultrahigh molecular weight polyethylene nanocomposites with osteoblast-like MG63 cells
Provedor de dados: 56
Autores: Reis,J.; Kanagaraj,S.; Fonseca,A.; Mathew,M.T.; Capela-Silva,F.; Potes,J.; Pereira,A.; Oliveira,M.S.A.; Simões,J.A.
Carbon nanotubes are highly versatile materials; new applications using them are continuously being developed. Special attention is being dedicated to the possible use of multiwalled carbon nanotubes in biomaterials contacting with bone. However, carbon nanotubes are also controversial in regards to effects exerted on living organisms. Carbon nanotubes can be used to improve the tribological properties of polymer/composite materials. Ultrahigh molecular weight polyethylene (UHMWPE) is a polymer widely used in orthopedic applications that imply wear and particle generation. We describe here the response of human osteoblast-like MG63 cells after 6 days of culture in contact with artificially generated particles from both UHMWPE polymer and multiwalled carbon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanocomposites; Carbon nanotubes; Wear particle; Osteoblast; Orthopedic; MG63 cells.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500009

Serogroups and virulence genotypes of Escherichia coli isolated from patients with sepsis
Provedor de dados: 56
Autores: Ananias,M.; Yano,T.
Sixty strains of Escherichia coli, isolated by hemoculture, from septicemic Brazilian patients were evaluated to determine their serogroup and invasivity to Vero cells. All 60 patients died within 2 days of hospitalization. Furthermore, the molecular study of the following extraintestinal pathogenic E. coli-associated virulence factor (VF) genes was performed by PCR: i) adhesins: type 1 fimbria (fimH), S fimbria (sfaD/E), P fimbria (papC and papG alleles) and afimbrial adhesin (afaB/C); ii) capsule K1/K5 (kpsMTII); iii) siderophores: aerobactin (iucD), yersiniabactin (fyuA) and salmochelin (iroN); iv) toxins hemolysin (hlyA), necrotizing cytotoxic factor type 1 (cnf1) and secreted autotransporter toxin (sat); v) miscellaneous: brain microvascular...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Sepsis; Serogroups; Virulence genotypes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000008

Histopathological analysis of rat mesentery as a method for evaluating neutrophil migration: differential effects of dexamethasone and pertussis toxin
Provedor de dados: 56
Autores: Brito,G.A.C.; Falcão,J.L.A.A.; Saraiva,S.N.R.; Lima,A.A.M.; Flores,C.A.; Ribeiro,R.A.
In the present study, histopathological analysis of rat mesentery was used to quantify the effect of two anti-inflammatory agents, dexamethasone (Dex) and pertussis toxin (Ptx), on leukocyte migration. The intravenous injection of Dex (1 mg/kg) and Ptx (1,200 ng) 1 h prior to the intraperitoneal injection of the inflammatory stimuli lipopolysaccharide (LPS) or formyl-methionyl-leucyl-phenylalanine (fMLP) significantly reduced the neutrophil diapedesis (LPS: Ptx = 0.86 ± 0.19 and Dex = 0.35 ± 0.13 vs saline (S) = 2.85 ± 0.59; fMLP: Ptx = 0.43 ± 0.09 and Dex 0.01 ± 0.01 vs S = 1.08 ± 0.15 neutrophil diapedesis/field) and infiltration (LPS: Ptx = 6.29 ± 1.4 and Dex = 3.06 ± 0.76 vs S = 15.94 ± 3.97; fMLP: Ptx = 3.85 ± 0.56 and Dex = 0.40 ± 0.16 vs S = 7.15 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutrophil migration; Pertussis toxin; LPS; Dexamethasone; Mesentery; Histopathological approach.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000013

Role of non-nitric oxide non-prostaglandin endothelium-derived relaxing factor(s) in bradykinin vasodilation
Provedor de dados: 56
Autores: Resende,A.C.; Ballejo,G.; Salgado,M.C.O.
The most conspicuous effect of bradykinin following its administration into the systemic circulation is a transient hypotension due to vasodilation. In the present study most of the available evidence regarding the mechanisms involved in bradykinin-induced arterial vasodilation is reviewed. It has become firmly established that in most species vasodilation in response to bradykinin is mediated by the release of endothelial relaxing factors following the activation of B2-receptors. Although in some cases the action of bradykinin is entirely mediated by the endothelial release of nitric oxide (NO) and/or prostacyclin (PGI2), a large amount of evidence has been accumulated during the last 10 years indicating that a non-NO/PGI2 factor accounts for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostaglandin; Bradykinin; Nitric oxide; Cytochrome P450; Potassium channels; Endothelium-dependent vasodilation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900017

The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction
Provedor de dados: 56
Autores: de-Souza,W.; de-Carvalho,T.U.; de-Melo,E.T.; Soares,C.P.; Coimbra,E.S.; Rosestolato,C.T.; Ferreira,S.R.; Vieira,M.
In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Confocal laser scanning microscopy; Host cell-parasite interaction; Trypanosoma cruzi; Toxoplasma gondii.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015

Effects of 5-aza-2′deoxycytidine on RECK gene expression and tumor invasion in salivary adenoid cystic carcinoma
Provedor de dados: 56
Autores: Zhou,X.Q.; Huang,S.Y.; Zhang,D.S.; Zhang,S.Z.; Li,W.G.; Chen,Z.W.; Wu,H.W.
Reversion-inducing cysteine-rich protein with kazal motifs (RECK), a novel tumor suppressor gene that negatively regulates matrix metalloproteinases (MMPs), is expressed in various normal human tissues but downregulated in several types of human tumors. The molecular mechanism for this downregulation and its biological significance in salivary adenoid cystic carcinoma (SACC) are unclear. In the present study, we investigated the effects of a DNA methyltransferase (DNMT) inhibitor, 5-aza-2′deoxycytidine (5-aza-dC), on the methylation status of the RECK gene and tumor invasion in SACC cell lines. Methylation-specific PCR (MSP), Western blot analysis, and quantitative real-time PCR were used to investigate the methylation status of the RECK gene and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RECK; Salivary adenoid cystic carcinoma; 5-aza-2′deoxycytidine; Methylation; Transwell assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300254

High baseline serum total and LDL cholesterol levels are associated with MDR1 haplotypes in Brazilian hypercholesterolemic individuals of European descent
Provedor de dados: 56
Autores: Rodrigues,A.C.; Rebecchi,I.M.M.; Bertolami,M.C.; Faludi,A.A.; Hirata,M.H.; Hirata,R.D.C.
The MDR1 gene encodes the P-glycoprotein, an efflux transporter with broad substrate specificity. P-glycoprotein has raised great interest in pharmacogenetics because it transports a variety of structurally divergent drugs, including lipid-lowering drugs. The synonymous single-nucleotide polymorphism C3435T and the nonsynonymous single-nucleotide polymorphism G2677T/A in MDR1 have been indicated as potential determinants of variability in drug disposition and efficacy. In order to evaluate the effect of G2677T/A and C3435T MDR1 polymorphisms on serum levels of lipids before and after atorvastatin administration, 69 unrelated hypercholesterolemic individuals from São Paulo city, Brazil, were selected and treated with 10 mg atorvastatin orally once daily for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MDR1 gene; Hypercholesterolemia; Statins; Single nucleotide polymorphism; Pharmacogenetics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900014

Fighting by sleep-deprived rats as a possible manifestation of panic: effects of sodium lactate
Provedor de dados: 56
Autores: Furlan,F.A.; Hoshino,K.
Increased fighting is an effect of desynchronized sleep deprivation (DSD) in rats, and recently this behavior has been suggested to be spontaneous panic and equivalent to panic disorder. In the present study we tested this hypothesis by evaluating the effect of sodium lactate on this aggressiveness, because this substance is recognized to induce spontaneous panic attacks in patients. A total of 186 male albino Wistar rats, 250-350 g, 90-120 days of age, were submitted to DSD (multiple platform method) for 0, 4, or 5 days. At the end of the deprivation period the rats were divided into subgroups respectively injected intraperitoneally with 1.86, 2.98 and 3.72 g/kg of 1 M sodium lactate, or 1.86 and 3.72 g/kg of 2 M sodium lactate. The control animals were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat; Sleep deprivation; Panic; Aggressiveness; Sodium lactate.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300010

Kaempferol inhibited VEGF and PGF expression and in vitro angiogenesis of HRECs under diabetic-like environment
Provedor de dados: 56
Autores: Xu,X.H.; Zhao,C.; Peng,Q.; Xie,P.; Liu,Q.H.
Diabetic retinopathy (DR) is one of the common and specific microvascular complications of diabetes. This study aimed to investigate the anti-angiogenic effect of kaempferol and explore its underlying molecular mechanisms. The mRNA expression level of vascular endothelial growth factor (VEGF) and placenta growth factor (PGF) and the concentrations of secreted VEGF and PGF were measured by qTR-PCR and ELISA assay, respectively. Human retinal endothelial cells (HRECs) proliferation, migration, and sprouting were measured by CCK-8 and transwell, scratching wound, and tube formation assays, respectively. Protein levels were determined by western blot. High glucose (25 mM) increased the mRNA expression levels of VEGF and PGF as well as the concentrations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetic retinopathy; Kaempferol; VEGF; PGF; Anti-angiogenic.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300605

From Escherichia coli heat-stable enterotoxin to mammalian endogenous guanylin hormones
Provedor de dados: 56
Autores: Lima,A.A.M.; Fonteles,M.C.
The isolation of heat-stable enterotoxin (STa) from Escherichia coli and cholera toxin from Vibrio cholerae has increased our knowledge of specific mechanisms of action that could be used as pharmacological tools to understand the guanylyl cyclase-C and the adenylyl cyclase enzymatic systems. These discoveries have also been instrumental in increasing our understanding of the basic mechanisms that control the electrolyte and water balance in the gut, kidney, and urinary tracts under normal conditions and in disease. Herein, we review the evolution of genes of the guanylin family and STa genes from bacteria to fish and mammals. We also describe new developments and perspectives regarding these novel bacterial compounds and peptide hormones that act in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat-stable enterotoxin; Guanylin; Guanylyl cyclase; Secretory diarrhea; Kidney function; Electrolyte and water balance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300179

Luteal phase of the menstrual cycle increases sweating rate during exercise
Provedor de dados: 56
Autores: Garcia,A.M.C.; Lacerda,M.G.; Fonseca,I.A.T.; Reis,F.M.; Rodrigues,L.O.C.; Silami-Garcia,E.
The present study evaluated whether the luteal phase elevation of body temperature would be offset during exercise by increased sweating, when women are normally hydrated. Eleven women performed 60 min of cycling exercise at 60% of their maximal work load at 32ºC and 80% relative air humidity. Each subject participated in two identical experimental sessions: one during the follicular phase (between days 5 and 8) and the other during the luteal phase (between days 22 and 25). Women with serum progesterone &gt;3 ng/mL, in the luteal phase were classified as group 1 (N = 4), whereas the others were classified as group 2 (N = 7). Post-exercise urine volume (213 ± 80 vs 309 ± 113 mL) and specific urine gravity (1.008 ± 0.003 vs 1.006 ± 0.002) changed (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thermoregulation; Sweating rate; Exercise; Menstrual cycle; Body temperature.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900014

Myosin V and iNOS expression is enhanced in J774 murine macrophages treated with IFN-gamma
Provedor de dados: 56
Autores: Reis,D.S.; Souza,M.A.; Mineo,J.R.; Espindola,F.S.
Actin-based motor protein requirements and nitric oxide (NO) production are important features of macrophage activity during phagocytosis or microbicidal processes. Different classes of myosins contribute directly or indirectly to phagocytosis by providing mechanical force for phagosome closure or organelle movement. Recent data have shown the presence of myosins IC, II, V and IXb in phagosomes of bone marrow-derived murine macrophages. In our investigation we demonstrated the presence of different classes of myosins in J774 macrophages. We also analyzed the effect of gamma interferon (IFN-gamma), with or without calcium ionophore or cytochalasin B, on myosins as well as on inducible nitric oxide synthase (iNOS) expression and NO production. Myosins IC,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myosins; Macrophages; IFN-gamma; INOS.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200009

A Brazilian family with hereditary inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia
Provedor de dados: 56
Autores: Fanganiello,R.D.; Kimonis,V.E.; Côrte,C.C.; Nitrini,R.; Passos-Bueno,M.R.
Inclusion body myopathy associated with Paget disease and frontotemporal dementia (IBMPFD) is a progressive and usually misdiagnosed autosomal dominant disorder. It is clinically characterized by a triad of features: proximal and distal myopathy, early onset Paget disease of bone (PDB), and frontotemporal dementia (FTD). It is caused by missense mutations in the valosin-containing protein (VCP) gene. We describe here the clinical and molecular findings of the first Brazilian family identified with IBMPFD. Progressive myopathy affecting the limb girdles was detected by clinical examination followed by muscle biopsy and creatine kinase measurement. PDB was suggested after anatomopathological bone examination and FTD was diagnosed by clinical,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Frontotemporal dementia; VCP gene mutations; Myopathy; Paget disease of bone.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400016

Chemokines, lymphocytes, and HIV
Provedor de dados: 56
Autores: Farber,J.M.
Chemokines are members of a family of more than 30 human cytokines whose best-described activities are as chemotactic factors for leukocytes and that are presumed to be important in leukocyte recruitment and trafficking. While many chemokines can act on lymphocytes, the roles of chemokines and their receptors in lymphocyte biology are poorly understood. The recent discoveries that chemokines can suppress infection by HIV-1 and that chemokine receptors serve, along with CD4, as obligate co-receptors for HIV-1 entry have lent urgency to studies on the relationships between chemokines and lymphocytes. My laboratory has characterized Mig and Crg-2/IP-10, chemokines that are induced by IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; and that specifically target...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Lymphoid cells; HIV-1; Receptors.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100002

Latent class analysis of attention and white matter correlation in children with attention-deficit/hyperactivity disorder
Provedor de dados: 56
Autores: Rossi,A.S.U.; Moura,L.M.; Miranda,M.C.; Muszkat,M.; Mello,C.B.; Bueno,O.F.A.
This study aimed to explore attentional patterns among children with inattentive attention-deficit/hyperactivity disorder (ADHD-I) and children with typical development (TD), using a latent class analysis (LCA). Patterns of brain connectivity were also explored. The sample comprised 29 ADHD-I and 29 TD matched children. An LCA was conducted to reclassify subjects according to their attentional performance, considering cognitive measures of attention and behavioral symptoms, regardless of group of origin. The new clusters were then compared in respect to brain white matter measurements (extracted from diffusion tensor imaging). Participants were rearranged in 2 new latent classes, according to their performance in an attention task and the results of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention-deficit/hyperactivity disorder; Attention; White matter; Pattern recognition; Diffusion tensor imaging.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100604

Diet plus insulin compared to diet alone in the treatment of gestational diabetes mellitus: a systematic review
Provedor de dados: 56
Autores: Giuffrida,F.M.A.; Castro,A.A.; Atallah,A.N.; Dib,S.A.
Fetuses of mothers with gestational diabetes mellitus are at increased risk to develop perinatal complications mainly due to macrosomia. However, in view of the marked heterogeneity of this disease, it seems difficult to set guidelines for diagnosis and treatment. This complicates the choice of assigning patients either to diet or to insulin therapy. Also of concern is how much benefit could be expected from insulin therapy in preventing fetal complications in these patients. In a systematic review of the literature assessing the efficacy of insulin in preventing macrosomia in fetuses of mothers with gestational diabetes, we found six randomized controlled trials comparing diet alone to diet plus insulin. The studies included a total of 1281 patients (644...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetes; Gestational; Diet therapy; Fetal macrosomia; Insulin; Meta-analysis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000004

Disaccharidase levels in normal epithelium of the small intestine of rats with iron-deficiency anemia
Provedor de dados: 56
Iron-deficiency anemia is the nutritional deficiency most frequently occurring throughout the world, which manifests as a complex systemic disease involving all cells, affecting enzyme activities and modifying protein synthesis. In view of these considerations, the objective of the present study was to determine the effects of iron-deficiency anemia on disaccharidases and on the epithelial morphokinetics of the jejunal mucosa. Newly weaned male Wistar rats were divided into 4 groups of 10 animals each: C6w received a standard ration containing 36 mg elemental iron per kg ration for 6 weeks; E6w received an iron-poor ration (5-8 mg/kg ration) for 6 weeks; C10w received an iron-rich ration (36 mg/kg ration) for 10 weeks; E10w received an iron-poor ration for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Disaccharidases; Anemia; Iron deficiency; Intestinal mucosa.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700005

Association of terpinolene and diclofenac presents antinociceptive and anti-inflammatory synergistic effects in a model of chronic inflammation
Provedor de dados: 56
Autores: Macedo,E.M.A.; Santos,W.C.; Sousa Neto,B.P.; Lopes,E.M.; Piauilino,C.A.; Cunha,F.V.M.; Sousa,D.P.; Oliveira,F.A.; Almeida,F.R.C.
Pharmacological treatment of inflammatory pain is usually done by administration of non-steroidal anti-inflammatory drugs (NSAIDs). These drugs present high efficacy, although side effects are common, especially gastrointestinal lesions. One of the pharmacological strategies to minimize such effects is the combination of drugs and natural products with synergistic analgesic effect. The monoterpene terpinolene (TPL) is a chemical constituent of essential oils present in many plant species, which have pharmacological activities, such as analgesic and anti-inflammatory. The association of ineffective doses of TPL and diclofenac (DCF) (3.125 and 1.25 mg/kg po, respectively) presented antinociceptive and anti-inflammatory effects in the acute (0, 1, 2, 3, 4, 5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Terpinolene; Inflammation; Antinociception; Pharmacological synergism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700602

Antibacterial and anti-inflammatory activities of an extract, fractions, and compounds isolated from Gochnatia pulchra aerial parts
Provedor de dados: 56
Autores: Lucarini,R.; Tozatti,M.G.; Silva,M.L.A.; Gimenez,V.M.M.; Pauletti,P.M.; Groppo,M.; Turatti,I.C.C.; Cunha,W.R.; Martins,C.H.G.
This paper reports on the in vitro antibacterial and in vivo anti-inflammatory properties of a hydroethanolic extract of the aerial parts of Gochnatia pulchra (HEGP). It also describes the antibacterial activity of HEGP fractions and of the isolated compounds genkwanin, scutellarin, apigenin, and 3,5-O-dicaffeoylquinic acid, as evaluated by a broth microdilution method. While HEGP and its fractions did not provide promising results, the isolated compounds exhibited pronounced antibacterial activity. The most sensitive microorganism was Streptococcus pyogenes, with minimum inhibitory concentration (MIC) values of 100, 50 and 25 µg/mL for genkwanin and the flavonoids apigenin and scutellarin, respectively. Genkwanin produced an MIC value of 25 µg/mL against...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gochnatia pulchra; Anti-inflammatory activity; Antibacterial activity; Medicinal plants; Proinflammatory cytokines.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900822

How are antibodies involved in the protective mechanism of susceptible mice infected with T. cruzi?
Provedor de dados: 56
Autores: Umekita,L.F.; Mota,I.
Host resistance to Trypanosoma cruzi is dependent on both natural and acquired immune responses. During the acute phase of the infection the presence of IFN-&lt;FONT FACE=Symbol&gt;g&lt;/font&gt;, TNF-&lt;FONT FACE=Symbol&gt;a&lt;/font&gt;, IL-12 and GM-CSF has been closely associated with resistance, whereas TGF-ß and IL-10 have been associated with susceptibility. Several investigators have demonstrated that antibodies are responsible for the survival of susceptible animals in the initial phase of infection and for the maintenance of low levels of parasitemia in the chronic phase. However, how this occurs is not yet understood. Our results and other data in the literature support the hypothesis that the protective role of antibodies in the acute phase of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Trypanosoma cruzi; Chronic mouse antibodies; Clearance; Cytokines; Acute phase; Susceptible mice.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300001

Clonal dissemination of VanA-type glycopeptide-resistant Enterococcus faecalis between hospitals of two cities located 100 km apart
Provedor de dados: 56
Autores: Moretti,M.L.; Bratfich,O.J.; Stucchi,R.B.; Levi,C.; Levin,A.S.; Duboc,G.M.; Vormittag,E.; Blum-Menezes,D.
Nosocomial dissemination of glycopeptide-resistant enterococci represents a major problem in hospitals worldwide. In Brazil, the dissemination among hospitals in the city of São Paulo of polyclonal DNA profiles was previously described for vancomycin-resistant Enterococcus faecium. We describe here the dissemination of VanA phenotype E. faecalis between two hospitals located in different cities in the State of São Paulo. The index outbreak occurred in a tertiary care university hospital (HCUSP) in the city of São Paulo and three years later a cluster caused by the same strain was recognized in two patients hospitalized in a private tertiary care hospital (CMC) located 100 km away in the interior of the state. From May to July 1999, 10 strains of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Vancomycin-resistant Enterococcus; Enterococcus faecalis; Tn1546; Nosocomial transmission; São Paulo.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900008

Cyclin D1 expression in prostate carcinoma
Provedor de dados: 56
Autores: Pereira,R.A.; Ravinal,R.C.; Costa,R.S.; Lima,M.S.; Tucci,S.; Muglia,V.F.; Dos Reis,R.B.; Silva,G.E.B.
The purpose of this study was to investigate the relationship between cyclin D1 expression and clinicopathological parameters in patients with prostate carcinoma. We assessed cyclin D1 expression by conventional immunohistochemistry in 85 patients who underwent radical prostatectomy for prostate carcinoma and 10 normal prostate tissue samples retrieved from autopsies. We measured nuclear immunostaining in the entire tumor area and based the results on the percentage of positive tumor cells. The preoperative prostate-specific antigen (PSA) level was 8.68±5.16 ng/mL (mean±SD). Cyclin D1 staining was positive (cyclin D1 expression in &gt;5% of tumor cells) in 64 cases (75.4%) and negative (cyclin D1 expression in ≤5% of tumor cells) in 21 cases (including 15...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Cyclin D1; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600515

Effect of air pollution on pediatric respiratory emergency room visits and hospital admissions
Provedor de dados: 56
Autores: Farhat,S.C.L.; Paulo,R.L.P.; Shimoda,T.M.; Conceição,G.M.S.; Lin,C.A.; Braga,A.L.F.; Warth,M.P.N.; Saldiva,P.H.N.
In order to assess the effect of air pollution on pediatric respiratory morbidity, we carried out a time series study using daily levels of PM10, SO2, NO2, ozone, and CO and daily numbers of pediatric respiratory emergency room visits and hospital admissions at the Children's Institute of the University of São Paulo Medical School, from August 1996 to August 1997. In this period there were 43,635 hospital emergency room visits, 4534 of which were due to lower respiratory tract disease. The total number of hospital admissions was 6785, 1021 of which were due to lower respiratory tract infectious and/or obstructive diseases. The three health end-points under investigation were the daily number of emergency room visits due to lower respiratory tract diseases,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Time-series; Poisson; Air pollution; Children; Respiratory morbidity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200011

Averting the legacy of kidney disease - focus on childhood
Provedor de dados: 56
Autores: Ingelfinger,J.R.; Kalantar-Zadeh,K.; Schaefer,F.
World Kidney Day 2016 focuses on kidney disease in childhood and the antecedents of adult kidney disease that can begin in earliest childhood. Chronic kidney disease (CKD) in childhood differs from that in adults, in that the largest diagnostic group among children includes congenital anomalies and inherited disorders, with glomerulopathies and kidney disease as a consequence of diabetes being relatively uncommon. In addition, many children with acute kidney injury will ultimately develop sequelae that may lead to hypertension and CKD in later childhood or in adult life. Children born early or who are small-for-date newborns have relatively increased risk for the development of CKD later in life. Persons with a high-risk birth and early childhood history...
Tipo: Info:eu-repo/semantics/article
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500303

H1 but not H2 histamine antagonist receptors mediate anxiety-related behaviors and emotional memory deficit in mice subjected to elevated plus-maze testing
Provedor de dados: 56
Autores: Serafim,K.R.; Kishi,M.S.; Canto-de-Souza,A.; Mattioli,R.
This study investigated the role of H1 and H2 receptors in anxiety and the retrieval of emotional memory using a Trial 1/Trial 2 (T1/T2) protocol in an elevated plus-maze (EPM). Tests were performed on 2 consecutive days, designated T1 and T2. Before T1, the mice received intraperitoneal injections of saline (SAL), 20 mg/kg zolantidine (ZOL, an H2receptor antagonist), or 8.0 or 16 mg/kg chlorpheniramine (CPA, an H1 receptor antagonist). After 40 min, they were subjected to the EPM test. In T2 (24 h later), each group was subdivided into two additional groups, and the animals from each group were re-injected with SAL or one of the drugs. In T1, the Student t-test showed no difference between the SAL and ZOL or 8 mg/kg CPA groups with respect to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorpheniramine; Zolantidine; Anxiety; Memory; Elevated plus-maze.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500440

Mutations of androgen receptor gene in Brazilian patients with male pseudohermaphroditism
Provedor de dados: 56
Autores: Cabral,D.F.; Maciel-Guerra,A.T.; Hackel,C.
We describe the identification of point mutations in the androgen receptor gene in five Brazilian patients with female assignment and behavior. The eight exons of the gene were amplified by the polymerase chain reaction (PCR) and analyzed for single-strand conformation polymorphism (SSCP) to detect the mutations. Direct sequencing of the mutant PCR products demonstrated single transitions in three of these cases: G&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;A in case 1, within exon C, changing codon 615 from Arg to His; G&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;A in case 2, within exon E, changing codon 752 from Arg to Gln, and C&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;T in case 3, within exon B, but without amino acid change.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Androgen receptor gene; Gene mutations; Androgen insensitivity syndromes; Male pseudohermaphroditism.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600008

Screening potential prognostic biomarkers of long non-coding RNAs for predicting the risk of chronic kidney disease
Provedor de dados: 56
Autores: Li,Na; Cui,Yan; Yin,Min; Liu,Feng.
Not much is known about the roles of long non-coding RNAs (lncRNAs) for chronic kidney disease (CKD). In this study, we included CKD patient cohorts and normal controls as a discovery cohort to identify putative lncRNA biomarkers associated with CKD. We first compared the lncRNA expression profiles of CKD patients with normal controls, and identified differentially expressed lncRNAs and mRNAs. Co-expression network based on the enriched differentially expressed mRNAs and lncRNAs was constructed using WGCNA to identify important modules related to CKD. A lncRNA-miRNA-mRNA pathway network based on the hub lncRNAs and mRNAs, related miRNAs, and overlapping pathways was further constructed to reveal putative biomarkers. A total of 821 significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Long non-coding RNAs; Chronic kidney disease; WGCNA; Prognostic biomarkers.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100608

Role of the hypothalamic pituitary adrenal axis in the control of the response to stress and infection
Provedor de dados: 56
Autores: McCann,S.M.; Antunes-Rodrigues,J.; Franci,C.R.; Anselmo-Franci,J.A.; Karanth,S.; Rettori,V.
The release of adrenocorticotropin (ACTH) from the corticotrophs is controlled principally by vasopressin and corticotropin-releasing hormone (CRH). Oxytocin may augment the release of ACTH under certain conditions, whereas atrial natriuretic peptide acts as a corticotropin release-inhibiting factor to inhibit ACTH release by direct action on the pituitary. Glucocorticoids act on their receptors within the hypothalamus and anterior pituitary gland to suppress the release of vasopressin and CRH and the release of ACTH in response to these neuropeptides. CRH neurons in the paraventricular nucleus also project to the cerebral cortex and subcortical regions and to the locus ceruleus (LC) in the brain stem. Cortical influences via the limbic system and possibly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corticotropin-releasing hormone (CRH); Vasopressin; Oxytocin; Atrial natriuretic peptide (ANP); ACTH; Cortisol; Norepinephrine; Acetyl choline; Nitric oxide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000001

Examining life-course influences on chronic disease: the importance of birth cohort studies from low- and middle- income countries. An overview
Provedor de dados: 56
Autores: Batty,G.D.; Alves,J.G.; Correia,J; Lawlor,D.A.
The objectives of this overview are to describe the past and potential contributions of birth cohorts to understanding chronic disease aetiology; advance a justification for the maintenance of birth cohorts from low- and middle-income countries (LMIC); provide an audit of birth cohorts from LMIC; and, finally, offer possible future directions for this sphere of research. While the contribution of birth cohorts from affluent societies to understanding disease aetiology has been considerable, we describe several reasons to anticipate why the results from such studies might not be directly applied to LMIC. More than any other developing country, Brazil has a tradition of establishing, maintaining and exploiting birth cohort studies. The clear need for a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Birth cohort; Chronic disease; Developing country; Epidemiology; Life-course.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900015

Estrogen receptor 1 gene polymorphisms and coronary artery disease in the Brazilian population
Provedor de dados: 56
Autores: Almeida,S.; Hutz,M.H.
We examined the association of three established single nucleotide polymorphisms, IVS1-397T&gt;C, IVS1-351A&gt;G, and +261G&gt;C, in the ESR1 gene with the prevalence and severity of coronary atherosclerosis in a southern Brazilian population of European ancestry. Three hundred and forty-one subjects (127 women and 214 men) with coronary artery disease (CAD) were classified as having significant disease (CAD+ patient group) when they showed 60% or more luminal stenosis in at least one coronary artery or major branch segment at angiography; patients with 10% or less luminal stenosis were considered to have minimal CAD (CAD- patient group). The control sample consisted of 142 subjects (79 women and 63 men) without significant disease, in whom coronary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESR1 polymorphisms; Coronary artery disease; Estrogen; Hormonal status.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400004

Berberine induces apoptosis via ROS generation in PANC-1 and MIA-PaCa2 pancreatic cell lines
Provedor de dados: 56
Autores: Park,S.H.; Sung,J.H.; Kim,E.J.; Chung,N.
Pancreatic cancer is the fourth leading cause of cancer death. Gemcitabine is widely used as a chemotherapeutic agent for the treatment of pancreatic cancer, but the prognosis is still poor. Berberine, an isoquinoline alkaloid extracted from a variety of natural herbs, possesses a variety of pharmacological properties including anticancer effects. In this study, we investigated the anticancer effects of berberine and compared its use with that of gemcitabine in the pancreatic cancer cell lines PANC-1 and MIA-PaCa2. Berberine inhibited cell growth in a dose-dependent manner by inducing cell cycle arrest and apoptosis. After berberine treatment, the G1 phase of PANC-1 cells increased by 10% compared to control cells, and the G1 phase of MIA-PaCa2 cells was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic cancer; Berberine; Gemcitabine; Apoptosis; Cell cycle arrest.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200111

Maintenance of the corneal epithelium is carried out by germinative cells of its basal stratum and not by presumed stem cells of the limbus
Provedor de dados: 56
Autores: Haddad,A.; Faria-e-Sousa,S.J.
The purpose of this investigation was to analyze the proliferative behavior of rabbit corneal epithelium and establish if any particular region was preferentially involved in epithelial maintenance. [3H]-thymidine was injected intravitreally into both normal eyes and eyes with partially scraped corneal epithelium. Semithin sections of the anterior segment were evaluated by quantitative autoradiography. Segments with active replication (on) and those with no cell division (off) were intermingled in all regions of the tissue, suggesting that the renewal of the epithelial surface of the cornea followed an on/off alternating pattern. In the limbus, heavy labeling of the outermost layers was observed, coupled with a few or no labeled nuclei in the basal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornea; Epithelium; Autoradiography; Germinative cells; Stem cells; Limbus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600470

Protective role of antioxidant vitamin E and catechin on idarubicin-induced cardiotoxicity in rats
Provedor de dados: 56
Autores: Kalender,S.; Kalender,Y.; Ates,A.; Yel,M.; Olcay,E.; Candan,S.
Idarubicin is an anthracycline antibiotic extensively used in acute leukemia. In the present study we investigated whether vitamin E and catechin can reduce the toxic effects of idarubicin. Vitamin E (200 IU kg-1 week-1), catechin (200 mg kg-1 week-1), idarubicin (5 mg kg-1 week-1), idarubicin + vitamin E (200 IU kg-1 week-1), and idarubicin + catechin (200 mg kg-1 week-1) combinations were given to male Sprague-Dawley rats weighing 210 to 230 g (N = 6/group). Idarubicin-treated animals exhibited a decrease in body and heart weight, a decrease in myocardial contractility, and changes in ECG parameters (P&lt;0.01). Catechin + idarubicin- and vitamin E + idarubicin-treated groups exhibited similar alterations, but changes were attenuated in comparison to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Idarubicin; Catechin; Vitamin E; Cardiotoxicity; Ultrastructure.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100017

The effects of cyclopiazonic acid on intracellular Ca2+ in aortic smooth muscle cells from DOCA-hypertensive rats
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200016

Thyroid function in post-weaning rats whose dams were fed a low-protein diet during suckling
Provedor de dados: 56
This study was designed to evaluate the thyroid and pituitary hormone levels in post-weaning rats whose dams were fed a low-protein diet during suckling (21 days). The dams and pups were divided into 2 groups: a control group fed a diet containing 22% protein that supplies the necessary amount of protein for the rat and is the usual content of protein in most commercial rat chow, and a diet group fed a low-protein (8%) diet in which the protein was substituted by an isocaloric amount of starch. After weaning all dams and pups received the 22% protein diet. Two hours before sacrifice of pups aged 21, 30 and 60 days, a tracer dose (0.6 µCi) of 125I was injected (ip) into each animal. Blood and thyroid glands of pups were collected for the determination of...
Palavras-chave: Thyroid; Protein malnutrition; Lactation; Thyrotropin; Thyroid hormones.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100020

Gender, race and socioeconomic influence on diagnosis and treatment of thyroid disorders in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Provedor de dados: 56
Autores: Olmos,R.D.; Figueiredo,R.C. de; Aquino,E.M.; Lotufo,P.A.; Bensenor,I.M.
Thyroid diseases are common, and use of levothyroxine is increasing worldwide. We investigated the influence of gender, race and socioeconomic status on the diagnosis and treatment of thyroid disorders using data from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil), a multicenter cohort study of civil servants (35-74 years of age) from six Brazilian cities. Diagnosis of thyroid dysfunction was by thyrotropin (TSH), and free thyroxine (FT4) if TSH was altered, and the use of specific medications. Multivariate logistic regression models were constructed using overt hyperthyroidism/hypothyroidism and levothyroxine use as dependent variables and sociodemographic characteristics as independent variables. The frequencies of overt hyper- and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid dysfunction; Treatment of thyroid disorders; Levothyroxine; Gender; Socioeconomic status; Race.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800751

Analysis of fracture healing in osteopenic bone caused by disuse: experimental study
Provedor de dados: 56
Autores: Paiva,A.G.; Yanagihara,G.R.; Macedo,A.P.; Ramos,J.; Issa,J.P.M.; Shimano,A.C.
Osteoporosis has become a serious global public health issue. Hence, osteoporotic fracture healing has been investigated in several previous studies because there is still controversy over the effect osteoporosis has on the healing process. The current study aimed to analyze two different periods of bone healing in normal and osteopenic rats. Sixty, 7-week-old female Wistar rats were randomly divided into four groups: unrestricted and immobilized for 2 weeks after osteotomy (OU2), suspended and immobilized for 2 weeks after osteotomy (OS2), unrestricted and immobilized for 6 weeks after osteotomy (OU6), and suspended and immobilized for 6 weeks after osteotomy (OS6). Osteotomy was performed in the middle third of the right tibia 21 days after tail...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Osteotomy; Fracture healing; Immobilization; Rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300708

Vagotomy ameliorates islet morphofunction and body metabolic homeostasis in MSG-obese rats
Provedor de dados: 56
Autores: Lubaczeuski,C.; Balbo,S.L.; Ribeiro,R.A.; Vettorazzi,J.F.; Santos-Silva,J.C.; Carneiro,E.M.; Bonfleur,M.L.
The parasympathetic nervous system is important for β-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic β-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline [control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-Cell; Obesity; Parasympathetic nervous system.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500447

Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
Provedor de dados: 56
Autores: López-López,P.; Martínez-López,M.C.; Boldo-León,X.M.; Hernández-Díaz,Y.; González-Castro,T.B.; Tovilla-Zárate,C.A.; Luna-Arias,J.P.
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amebiasis; Neglected diseases; Diagnostic; PCR; DGGE; Adh112 gene.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607

Ischemia preconditioning is neuroprotective in a rat cerebral ischemic injury model through autophagy activation and apoptosis inhibition
Provedor de dados: 56
Autores: Xia,D.Y.; Li,W.; Qian,H.R.; Yao,S.; Liu,J.G.; Qi,X.K.
Sublethal ischemic preconditioning (IPC) is a powerful inducer of ischemic brain tolerance. However, its underlying mechanisms are still not well understood. In this study, we chose four different IPC paradigms, namely 5 min (5 min duration), 5×5 min (5 min duration, 2 episodes, 15-min interval), 5×5×5 min (5 min duration, 3 episodes, 15-min intervals), and 15 min (15 min duration), and demonstrated that three episodes of 5 min IPC activated autophagy to the greatest extent 24 h after IPC, as evidenced by Beclin expression and LC3-I/II conversion. Autophagic activation was mediated by the tuberous sclerosis type 1 (TSC1)-mTor signal pathway as IPC increased TSC1 but decreased mTor phosphorylation. Terminal deoxynucleotidyl transferase dUTP nick end...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemic preconditioning; Autophagy; Apoptosis; Focal cerebral ischemic reperfusion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700580

Comparative performance of two air samplers for monitoring airborne fungal propagules
Provedor de dados: 56
Autores: Távora,L.G.F.; Gambale,W.; Heins-Vaccari,E.M.; Arriagada,G.L.H.; Lacaz,C.S.; Santos,C.R.; Levin,A.S.
Many studies have attempted to evaluate the importance of airborne fungi in the development of invasive fungal infection, especially for immunocompromised hosts. Several kinds of instruments are available to quantitate fungal propagule levels in air. We compared the performance of the most frequently used air sampler, the Andersen sampler with six stages, with a portable one, the Reuter centrifugal sampler (RCS). A total of 84 samples were analyzed, 42 with each sampler. Twenty-eight different fungal genera were identified in samples analyzed with the Andersen instrument. In samples obtained with the RCS only seven different fungal genera were identified. The three most frequently isolated genera in samples analyzed with both devices were Penicillium,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Andersen sampler; Reuter centrifugal sampler; Air sampler; Fungal contamination; Indoor air; Hospital air.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500008

Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development
Provedor de dados: 56
Autores: Feng,D.Q.; Zhou,Y.; Ling,B.; Gao,T.; Shi,Y.Y.; Wei,H.M.; Tian,Z.G.
Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the current study, we investigated the effect of CM on the events of mouse oocyte activation, namely oscillations of cytosolic calcium concentration ([Ca²+]i), meiosis resumption, pronucleus formation, and parthenogenetic development. The surface markers of MSCs were identified with a fluorescence-activated cell sorter. The dynamic changes of the spindle and formation of pronuclei were examined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Oocyte activation; Parthenogenesis; Calcium; Cytochalasin B.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600006

Cantharidin suppressed breast cancer MDA-MB-231 cell growth and migration by inhibiting MAPK signaling pathway
Provedor de dados: 56
Autores: Gu,X.-D.; Xu,L.-L; Zhao,H.; Gu,J.-Z; Xie,X.-H.
As an active constituent of the beetle Mylabris used in traditional Chinese medicine, cantharidin is a potent and selective inhibitor of protein phosphatase 2A (PP2A) that plays a crucial role in cell cycle progression, apoptosis, and cell fate. The role and possible mechanisms exerted by cantharidin in cell growth and metastasis of breast cancer were investigated in this study. Cantharidin was found to inhibit cell viability and clonogenic potential in a time- and dose-dependent manner. Cell cycle analysis revealed that cell percentage in G2/M phase decreased, whereas cells in S and G1 phases progressively accumulated with the increasing doses of cantharidin treatment. In a xenograft model of breast cancer, cantharidin inhibited tumor growth in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cantharidin; Breast cancer; ERK/MAPK pathway; Growth; Migration; Invasion.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700608

Acute leukemia in early childhood
Provedor de dados: 56
Autores: Emerenciano,M.; Koifman,S.; Pombo-de-Oliveira,M.S.
Acute leukemia in early childhood is biologically and clinically distinct. The particular characteristics of this malignancy diagnosed during the first months of life have provided remarkable insights into the etiology of the disease. The pro-B, CD10 negative immunophenotype is typically found in infant acute leukemia, and the most common genetic alterations are the rearrangements of the MLL gene. In addition, the TEL/AML1 fusion gene is most frequently found in children older than 24 months. A molecular study on a Brazilian cohort (age range 0-23 months) has detected TEL/AML1+ve (N = 9), E2A/PBX1+ve (N = 4), PML/RARA+ve (N = 4), and AML1/ETO+ve (N = 2) cases. Undoubtedly, the great majority of genetic events occurring in these patients arise prenatally....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infant acute leukemia; MLL; Acute lymphoblastic leukemia; Acute myeloid leukemia; Maternal exposures; Molecular and exploratory epidemiology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600002

Cl- and regulation of pH by MDCK-C11 cells
Provedor de dados: 56
Autores: Tararthuch,A.L.; Fernandez,R.; Malnic,G.
The interaction between H+ extrusion via H+-ATPase and Cl- conductance was studied in the C11 clone of MDCK cells, akin to the intercalated cells of the collecting duct. Cell pH (pHi) was measured by fluorescence microscopy using the fluorescein-derived probe BCECF-AM. Control recovery rate measured after a 20 mM NH4Cl acid pulse was 0.136 ± 0.008 pH units/min (dpHi/dt) in Na+ Ringer and 0.032 ± 0.003 in the absence of Na+ (0 Na+). With 0 Na+ plus the Cl- channel inhibitor NPPB (10 µM), recovery was reduced to 0.014 ± 0.001 dpHi/dt. 8-Br-cAMP, known to activate CFTR Cl- channels, increased dpHi/dt in 0 Na+ to 0.061 ± 0.009 and also in the presence of 46 nM concanamycin and 50 µM Schering 28080. Since it is thought that the Cl- dependence of H+-ATPase might...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell pH; CAMP; ATP; H+-ATPase; Valinomycin; Cl-.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500012

Comparative study of IgA nephropathy with and without crescents
Provedor de dados: 56
Autores: Bitencourt-Dias,C.; Bahiense-Oliveira,M.; Saldanha,L.B.; Barros,R.T.; Woronik,V.
Glomerular crescents were analyzed as a prognostic factor in retrospectively reviewed data from 144 patients with biopsy-proven IgA nephropathy. Crescents were found in 26 (18%) patients, and detected in 2 to 100% of glomeruli in each specimen. In 5% of the patients more than 50% of the glomeruli were affected. Thirty patients with IgA nephropathy without crescents were studied as a control group. Mean age was 30.3 ± 9.4 and 30.2 ± 12.0 years for the patients with and without crescents, respectively, and males prevailed in both groups. The length of follow-up was 23.2 ± 41.6 months for patients with crescents and 29.3 ± 35.3 months for patients without crescents. Eighty percent of the patients with crescents were hypertensive, compared to 27% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgA nephropathy; Crescents; Renal failure; End-stage renal disease; Creatinine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900012

Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals
Provedor de dados: 56
Autores: Mo,Hui; Zhang,Ning; Li,Huifu; Li,Fan; Pu,Rong.
Cuscuta chinensis Lam. (Convolvulaceae) is an important herbal medicine widely used to improve sexual function, treat osteoporosis, and prevent aging, and has been reported to exhibit anti-osteoporotic effects in vitro. However, the activity of Cuscuta chinensis Lam. on glucocorticoid-induced osteoporosis still remains unclear. The present study aimed to assess the protective effect and the underlying mechanism of action of Cuscuta chinensis extract (CCE) against glucocorticoid-induced osteoporosis in vivo. Sprague-Dawley rats were randomly divided into four groups as follows: control group, osteoporosis group, and 2 CCE-treated osteoporosis groups (100 mg·kg-1·day-1). Blood samples and femur bones were collected for immunohistochemistry, biochemical, mRNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dexamethasone; Oxidative status; Bone formation; Anti-osteoporosis; RunX2.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200612

Effect of hyperoxia on the intestinal IgA secretory component in neonatal rats and on intestinal epithelial cells in vitro
Provedor de dados: 56
Autores: Li,J.J.
Oxygen therapy is essential for the treatment of some neonatal critical care conditions but its extrapulmonary effects have not been adequately investigated. We therefore studied the effects of various oxygen concentrations on intestinal epithelial cell function. In order to assess the effects of hyperoxia on the intestinal immunological barrier, we studied two physiological changes in neonatal rats exposed to hyperoxia: the change in intestinal IgA secretory component (SC, an important component of SIgA) and changes in intestinal epithelial cells. Immunohistochemistry and Western blot were used to detect changes in the intestinal tissue SC of neonatal rats. To detect intestinal epithelial cell growth, cells were counted, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal epithelial cells; Cell growth; Secretory component; Hyperoxia; Secretory IgA.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100004

Construction of a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen and mouse 4-1BBL genes and its effect on dendritic cells
Provedor de dados: 56
Autores: Weng,Xiaodong; Kuang,Youlin; Liu,Xiuheng; Chen,Zhiyuan; Zhu,Hengcheng; Chen,Hui; Jiang,Botao; Shen,Hao.
Our aim was to construct a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen (tPSMA) and mouse 4-1BBL genes and to determine its effect on dendritic cells (DCs) generated from bone marrow suspensions harvested from C57BL/6 mice for which the effect of 4-1BBL on DCs is not clear, especially during DCs processing tumor-associated antigen. Replication deficient adenovirus AdMaxTM Expression System was used to construct recombinant adenovirus Ad-tPSMA-internal ribosome entry site-mouse 4-1BBL (Ad-tPSMA-IRES-m4-1BBL) and Ad-enhanced green fluorescent protein. Day 7 proliferating DC aggregates generated from C57BL/6 mice were collected as immature DCs and further mature DCs were obtained by lipopolysaccharide activated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; PSMA; Co-stimulatory molecule; 4-1BBL; Adenovirus; Dendritic cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300002

Schwann cells for spinal cord repair
Provedor de dados: 56
Autores: Oudega,M.; Moon,L.D.F.; de Almeida Leme,R.J.
The complex nature of spinal cord injury appears to demand a multifactorial repair strategy. One of the components that will likely be included is an implant that will fill the area of lost nervous tissue and provide a growth substrate for injured axons. Here we will discuss the role of Schwann cells (SCs) in cell-based, surgical repair strategies of the injured adult spinal cord. We will review key studies that showed that intraspinal SC grafts limit injury-induced tissue loss and promote axonal regeneration and myelination, and that this response can be improved by adding neurotrophic factors or anti-inflammatory agents. These results will be compared with several other approaches to the repair of the spinal cord. A general concern with repair strategies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Implantation; Regeneration; Neurotrophic factors; Clinical trial; Rehabilitation; Gene profiling.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600003

Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response
Provedor de dados: 56
Autores: Dang,N.N.; Pang,S.G.; Song,H.Y.; An,L.G.; Ma,X.L.
The objective of this study was to investigate whether a single defect in skin barrier function simulated by filaggrin silencing could induce Th2-predominant inflammation. Filaggrin gene expression was silenced in cultured normal human epidermal keratinocytes (NHEKs) using small hairpin RNA (shRNA, GTTGGCTCAAGCATATTATTT). The efficacy of silencing was confirmed by polymerase chain reaction (PCR) and Western blotting. Filaggrin-silenced cells (LV group), shRNA control cells (NC group), and noninfected cells (Blank group) were evaluated. The expression of cornified cell envelope-related proteins, including cytokeratin (CK)-5, -10, -14, loricrin, involucrin, and transglutaminase (TGM)-1, was detected by Western blotting. Interleukins (IL)-2, IL-4, IL-5,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atopic dermatitis; T helper 2 cytokines; Filaggrin silencing; Pathogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100039

Adenosine A1 receptor-mediated inhibition of in vitro prolactin secretion from the rat anterior pituitary
Provedor de dados: 56
Autores: Picanço-Diniz,D.L.W.; Valença,M.M.; Antunes-Rodrigues,J.
In previous studies, we demonstrated biphasic purinergic effects on prolactin (PRL) secretion stimulated by an adenosine A2 agonist. In the present study, we investigated the role of the activation of adenosine A1 receptors by (R)-N6-(2-phenylisopropyl)adenosine (R-PIA) at the pituitary level in in vitro PRL secretion. Hemipituitaries (one per cuvette in five replicates) from adult male rats were incubated. Administration of R-PIA (0.001, 0.01, 0.1, 1, and 10 µM) induced a reduction of PRL secretion into the medium in a U-shaped dose-response curve. The maximal reduction was obtained with 0.1 µM R-PIA (mean ± SEM, 36.01 ± 5.53 ng/mg tissue weight (t.w.)) treatment compared to control (264.56 ± 15.46 ng/mg t.w.). R-PIA inhibition (0.01 µM = 141.97 ± 15.79...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclopentyltheophylline; Dibutyryl cAMP; R-PIA; Pertussis toxin; Cholera toxin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100013

Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults
Provedor de dados: 56
Autores: Kawamoto,E.M.; Gleichmann,M.; Yshii,L.M.; Lima,L. de Sá; Mattson,M.P.; Scavone,C.
Wnt proteins are involved in tissue development and their signaling pathways play an important role during embryogenesis. Wnt signaling can promote cell survival, which is beneficial for neurons, but could also lead to tumor development in different tissues. The present study investigated the effects of a Wnt protein on the susceptibility of a neural tumor cell line (PC12 cells) to the cytotoxic compounds ferrous sulfate (10 mM), staurosporine (100 and 500 nM), 3-nitropropionic acid (5 mM), and amyloid β-peptide (Aβ25-35; 50 µM). Cells (1 x 10(6) cells/mL) were treated with the Wnt-3a recombinant peptide (200 ng/mL) for 24 h before exposure to toxic insults. The Wnt-3a protein partially protected PC12 cells, with a 6-15% increase in cell viability in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wnt-3a; PC12 cells; Beta-catenin; NF-κB.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100010

Two new mixed-ligand coordination polymers based on multi-N chelating ligand inhibit YAP expression and induce caspase-mediated spinal tumor cell apoptosis
Provedor de dados: 56
Autores: Sun,Yi Fu; Shao,Li Wei; Chen,Qi; Gao,Xu; Li,Fang; Wu,Chang Yan.
Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The cell viability curves showed that the two compounds have anti-proliferation activity on spinal tumor cells, and the activity of compound 1 is higher than compound 2. The annexin V-FITC/PI assay and western...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coordination polymers; Glioma cell strains; Cytotoxicity; Apoptosis; Immunoblotting.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500608

Morphometry and acetylcholinesterase activity of the myenteric plexus of the wild mouse Calomys callosus
Provedor de dados: 56
The myenteric plexus of the digestive tract of the wild mouse Calomys callosus was examined using a histochemical method that selectively stains nerve cells, and the acetylcholinesterase (AChE) histochemical technique in whole-mount preparations. Neuronal density was 1,500 ± 116 neurons/cm2 (mean ± SEM) in the esophagus, 8,900 ± 1,518 in the stomach, 9,000 ± 711 in the jejunum and 13,100 ± 2,089 in the colon. The difference in neuronal density between the esophagus and other regions was statistically significant. The neuron profile area ranged from 45 to 1,100 µm2. The difference in nerve cell size between the jejunum and other regions was statistically significant. AChE-positive nerve fibers were distributed within the myenteric plexus which is formed by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myenteric plexus; Wild mouse; Calomys callosus; Morphometry; Histochemistry.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500009

Galunisertib (LY2157299), a transforming growth factor-β receptor I kinase inhibitor, attenuates acute pancreatitis in rats
Provedor de dados: 56
Autores: Liu,X.; Yu,M.; Chen,Y.; Zhang,J.
Galunisertib (LY2157299), a selective ATP-mimetic inhibitor of TGF-β receptor I (TGF-βRI), is the only known TGF-β pathway inhibitor. In the present study, we investigated the effect of galunisertib on taurocholate (TAC)-induced acute pancreatitis (AP) in rats, and the role of TGF-β and NF-κB signaling pathways. AP was induced by infusion of TAC into the pancreatic duct of Sprague-Dawley male rats (n=30). The rats (220±50 g) were administered galunisertib intragastrically [75 mg·kg-1·day-1 for 2 days (0 and 24 h)]. Serum IL-1β, IL-6, TNF-α, amylase (AMY), lipase (LIP), and myeloperoxidase (MPO) levels were measured by ELISA. NF-κB activity was detected by electrophoretic mobility shift assay (EMSA); NF-κBp65 and TGF-β1 proteins, as well as TGF-βRI and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute pancreatitis; TGFβ1; NF-κB; Galunisertib.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900705

High mobility group box 1 as a mediator of endotoxin administration after hemorrhagic shock-primed lung injury
Provedor de dados: 56
Autores: Guo,F.; Shi,Y.; Xu,H.; Ding,J.
High mobility group box 1 (HMGB1) was discovered as a novel late-acting cytokine that contributes to acute lung injury (ALI). However, the contribution of HMGB1 to two-hit-induced ALI has not been investigated. To examine the participation of HMGB1 in the pathogenesis of ALI caused by the two-hit hypothesis, endotoxin was injected intratracheally in a hemorrhagic shock-primed ALI mouse model. Concentrations of HMGB1 in the lung of the shock group were markedly increased at 16 h (1.63 ± 0.05, compared to the control group: 1.02 ± 0.03; P &lt; 0.05), with the highest concentration being observed at 24 h. In the sham/lipopolysaccharide group, lung HMGB1 concentrations were found to be markedly increased at 24 h (1.98 ± 0.08, compared to the control group:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lung injury; High mobility group box 1; Two-hit-induced ALI; Hemorrhagic shock; Endotoxin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900006

Bradykinin enhances membrane electrical activity of pancreatic beta cells in the presence of low glucose concentrations
Provedor de dados: 56
Autores: Moura,A.S.
In most of cells bradykinin (BK) induces intracellular calcium mobilization. In pancreatic beta cells intracellular calcium is a major signal for insulin secretion. In these cells, glucose metabolism yields intracellular ATP which blocks membrane potassium channels. The membrane depolarizes, voltage-dependent Ca2+ channels are activated and the intracellular calcium load allows insulin secretion. Repolarization occurs due to activation of the Ca2+-dependent K+ channel. The insulin secretion depends on the integrity of this oscillatory process (bursts). Therefore, we decided to determine whether BK (100 nM) induces bursts in the presence of a non-stimulatory glucose concentration (5.6 mM). During continuous membrane voltage recording, our results showed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Membrane potential; Pancreatic beta cell; Bradykinin; Calcium.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900016

Moderate intensity physical training accelerates healing of full-thickness wounds in mice
Provedor de dados: 56
Autores: Zogaib,F.G.; Monte-Alto-Costa,A.
Physical training influences the cells and mediators involved in skin wound healing. The objective of this study was to determine the changes induced by different intensities of physical training in mouse skin wound healing. Ninety male C57BL6 mice (8 weeks old, 20-25 g) were randomized into three physical training groups: moderate (70% VO2max), high (80% VO2max), and strenuous intensity (90% VO2max). Animals trained on a motorized treadmill for 8 weeks (Elesion: physical training until the day of excisional lesion, N = 10) or 10 weeks (Eeuthan: physical training for 2 additional weeks after excisional lesion until euthanasia, N = 10), five times/week, for 45 min. Control groups (CG) trained on the treadmill three times/week only for 5 min (N = 10). In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical training; Wound healing; Myofibroblast; Skin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000008

New paradigms and ethics
Provedor de dados: 56
Autores: Castro,L.A.B. de.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500001

p16INK4 expression in precursor lesions of squamous cell cervical cancer related to the presence of HPV-DNA
Provedor de dados: 56
Autores: Godoy,A.E.G.; Mandelli,J.; Oliveira,F.H.; Calegari,S.; Moura,L.B.; Serafini,E.P.
The purpose of the present study was to identify the expression of p16INK4 in cervical cancer precursor lesions by immunohistochemistry and to correlate it with lesion grade and presence of human papillomavirus (HPV) infection. Cervical specimens from 144 women seen consecutively at the gynecology outpatient clinic of our institution from December 2003 to May 2005 were analyzed by cytopathology, histopathology, polymerase chain reaction for HPV-DNA, and p16INK4 immunostaining. Histologically normal biopsies, HPV-DNA negative by polymerase chain reaction, were used as control. HPV-DNA prevalence, including the control group, was 68.1% and the prevalence of p16INK4 expression was 55.0%. The percentage of cells stained by p16INK4 ranged from 10 to 100%, both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P16INK4; Cervical cancer; Human papillomavirus; Immunohistochemistry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700006

Endothelial dysfunction in cardiovascular and endocrine-metabolic diseases: an update
Provedor de dados: 56
Autores: Davel,A.P.; Wenceslau,C.F.; Akamine,E.H.; Xavier,F.E.; Couto,G.K.; Oliveira,H.T.; Rossoni,L.V.
The endothelium plays a vital role in maintaining circulatory homeostasis by the release of relaxing and contracting factors. Any change in this balance may result in a process known as endothelial dysfunction that leads to impaired control of vascular tone and contributes to the pathogenesis of some cardiovascular and endocrine/metabolic diseases. Reduced endothelium-derived nitric oxide (NO) bioavailability and increased production of thromboxane A2, prostaglandin H2 and superoxide anion in conductance and resistance arteries are commonly associated with endothelial dysfunction in hypertensive, diabetic and obese animals, resulting in reduced endothelium-dependent vasodilatation and in increased vasoconstrictor responses. In addition, recent studies have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelial dysfunction; Hypertension; Sympathetic hyperactivity; Diabetes; Obesity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900014

Glycosaminoglycans affect the interaction of human plasma kallikrein with plasminogen, factor XII and inhibitors
Provedor de dados: 56
Autores: Gozzo,A.J.; Nunes,V.A.; Nader,H.B.; Dietrich,C.P.; Carmona,A.K.; Sampaio,M.U.; Sampaio,C.A.M.; Araújo,M.S.
Human plasma kallikrein, a serine proteinase, plays a key role in intrinsic blood clotting, in the kallikrein-kinin system, and in fibrinolysis. The proteolytic enzymes involved in these processes are usually controlled by specific inhibitors and may be influenced by several factors including glycosaminoglycans, as recently demonstrated by our group. The aim of the present study was to investigate the effect of glycosaminoglycans (30 to 250 µg/ml) on kallikrein activity on plasminogen and factor XII and on the inhibition of kallikrein by the plasma proteins C1-inhibitor and antithrombin. Almost all available glycosaminoglycans (heparin, heparan sulfate, bovine and tuna dermatan sulfate, chondroitin 4- and 6-sulfates) reduced (1.2 to 3.0 times) the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycosaminoglycans; Human plasma kallikrein; Plasminogen; Factor XII; C1-inhibitor; Antithrombin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800011

Ghrelin plasma levels, gastric ghrelin cell density and bone mineral density in women with rheumatoid arthritis
Provedor de dados: 56
Autores: Maksud,F.A.N.; Kakehasi,A.M.; Guimarães,M.F.B.R.; Machado,C.J.; Barbosa,A.J.A.
Generalized bone loss can be considered an extra-articular manifestation of rheumatoid arthritis (RA) that may lead to the occurrence of fractures, resulting in decreased quality of life and increased healthcare costs. The peptide ghrelin has demonstrated to positively affect osteoblasts in vitro and has anti-inflammatory actions, but the studies that correlate ghrelin plasma levels and RA have contradictory results. We aimed to evaluate the correlation between total ghrelin plasma levels, density of ghrelin-immunoreactive cells in the gastric mucosa, and bone mineral density (BMD) in twenty adult women with established RA with 6 months or more of symptoms (mean age of 52.70±11.40 years). Patients with RA presented higher ghrelin-immunoreactive cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ghrelin; Gastric mucosa; Bone metabolism; Osteoporosis; Rheumatoid arthritis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600704

Low expression of APAF-1XL in acute myeloid leukemia may be associated with the failure of remission induction therapy
Provedor de dados: 56
Autores: Benites,B.D.; Fattori,A.; Hackel,C.; Lorand-Metze,I.; De Souza,C.A.; Schulz,E.; Costa,F.F.; Saad,S.T.O.
Apoptotic protease activating factor 1 (APAF-1) has a critical role in the regulation of apoptosis. In the present study, the mRNA expression analysis of different APAF-1 transcripts (APAF-1S, APAF-1LC, APAF-1LN, and APAF-1XL) was analyzed in bone marrow samples from 37 patients with acute myeloid leukemia (newly diagnosed, with no previous treatment). APAF-1XL and APAF-1LN transcripts (with and without an extra WD-40 repeat region, respectively) were detected in all samples, although the major form expressed was APAF-1XL in 65% of the samples (group 1), while 35% of the samples expressed primarily APAF-1LN (group 2). Only 46% of the patients presented complete remission in response to remission induction therapy (represented by less than 5% marrow blasts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: APAF-1; Acute myeloid leukemia; Apoptosis; Chemotherapy resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700004

In vitro cultivation of canine multipotent mesenchymal stromal cells on collagen membranes treated with hyaluronic acid for cell therapy and tissue regeneration
Provedor de dados: 56
Autores: Wodewotzky,T.I.; Lima-Neto,J.F.; Pereira-Júnior,O.C.M.; Sudano,M.J.; Lima,S.A.F.; Bersano,P.R.O.; Yoshioka,S.A.; Landim-Alvarenga,F.C.
Support structures for dermal regeneration are composed of biodegradable and bioresorbable polymers, animal skin or tendons, or are bacteria products. The use of such materials is controversial due to their low efficiency. An important area within tissue engineering is the application of multipotent mesenchymal stromal cells (MSCs) to reparative surgery. The combined use of biodegradable membranes with stem cell therapy may lead to promising results for patients undergoing unsuccessful conventional treatments. Thus, the aim of this study was to test the efficacy of using membranes composed of anionic collagen with or without the addition of hyaluronic acid (HA) as a substrate for adhesion and in vitro differentiation of bone marrow-derived canine MSCs. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen scaffolds; Biomaterials; Hyaluronic acid; Mesenchymal stromal cells; Tissue engineering; Cell culture.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200008

The starch from Solanum lycocarpum St. Hill. fruit is not a hypoglycemic agent
Provedor de dados: 56
Autores: Oliveira,A.C.P.; Endringer,D.C.; Araújo,R.J.P.; Brandão,M.G.L.; Coelho,M.M.
We have investigated the hypoglycemic effect induced by the starch obtained from the unripe fruits of Solanum lycocarpum (Solanaceae). Per os administration of the starch (1000 or 2000 mg/kg, twice daily for 7 days, N = 6) did not change glycemia levels of nondiabetic female Swiss mice weighing 25-30 g. In streptozotocin-induced diabetic mice, similar treatment with the starch did not change the elevated glycemia 3 h after the last dose (diabetic treated with saline = 288 ± 17/309 ± 18; starch 1000 mg/kg = 295 ± 33; starch 2000 mg/kg = 258 ± 37; N = 5). In animals fasted for 15 h, per os administration of glucose (600 mg/kg) significantly increased glycemia 1 h later. Previous (-30 min) treatment of the animals with the starch (1000 or 2000 mg/kg; N = 5)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Solanum lycocarpum St. Hill.; Glycoalkaloids; Starch fraction; Diabetes mellitus; Hypoglycemic effect.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400017

Experimental myocardial hypertrophy induced by a minimally invasive ascending aorta coarctation
Provedor de dados: 56
Autores: Martins,A.S.; Aguilera,N.W.; Matsubara,B.B.; Bregagnollo,E.A.
Ascending aorta coarctation was produced by a minimally invasive technique in rabbits. Animal mortality was 5%. Morphometric and hemodynamic parameters were evaluated. A parabiotically isolated heart model was used to assess the hemodynamic parameters. Left ventricular weight/body weight ratio and muscle area showed clear evidence of hypertrophy when compared to control. The hemodynamic changes in the isolated heart model suggested decreased diastolic and systolic function in the coarcted group. The present model produced hypertrophy with low mortality rates as a result of its less invasive nature.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ventricular hypertrophy; Ventricular function; Experimental model.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300017

Therapeutic effects of Sophora moorcroftiana alkaloids in combination with albendazole in mice experimentally infected with protoscolices of Echinococcus granulosus
Provedor de dados: 56
Autores: Ma,X.M.; Bao,G.SH.; Wan,J.M.; Liao,D.J.; Yin,SH.F.; Meng,X.Q.; Zhou,G.K.; Lu,X.M.; Li,H.Y.
The objective of the present study was to determine if the combination of alkaloids from Sophora moorcroftiana seeds and albendazole might be effective in the treatment of experimental echinococcosisin female NIH mice (6 weeks old and weighing 18-20 g, N = 8 in each group) infected withprotoscolices of Echinococcus granulosus. Viable protoscolices (N = 6 x 103) were cultured in vitro in 1640 medium and mortality was calculated daily. To determine the in vivo efficacy, mice were inoculated intraperitoneally with viable protoscolices and then treated once daily by gavage for three months with the alkaloids (50 mg kg-1 day-1) and albendazole (50 mg kg-1 day-1), separately and in combination (both alkaloids at 25 mg kg-1 day-1 and albendazole at 25 mg kg-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sophora moorcroftiana; Echinococcus granulosus; Alkaloids; Cytokines; Ethnobotany; Therapeutics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000014

Reproductive experience influences grooming behavior during pregnancy in rats
Provedor de dados: 56
Autores: Serafim,A.P.; Felicio,L.F.
The pregnancy-induced increase in self-licking observed in rats is important for mammary gland development and lactation. Reproductive experience has epidemiologial implications such as a decrease in the incidence of mammary gland cancer in women and it also influences various behavioral, neurochemical and endocrine parameters. The aim of the present study was to investigate the influence of reproductive experience on grooming behavior patterns during pregnancy in rats. Self-grooming behavior was measured in age-matched virgin, primi- and multigravid (days 7, 8, 9, 19, and 20 of pregnancy) rats. General grooming (head, forelimbs and shoulders) was not significantly different among virgin, primi- and multigravid rats during pregnancy. Confirming previous...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Self-licking; Parity; Lactation; Mammary gland; Prolactin; Dopamine.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300016

Effects of 60Co gamma radiation on crotamine
Provedor de dados: 56
Autores: Boni-Mitake,M.; Costa,H.; Spencer,P.J.; Vassilieff,V.S.; Rogero,J.R.
Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide (pI 10.3) from Crotalus durissus terrificus venom composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of the present study was to carry out a biochemical study and a toxic activity assay on native and irradiated crotamine. Crotamine was purified from C.d. terrificus venom by Sephadex G-100 gel filtration followed by ion-exchange chromatography, and irradiated at 2 mg/ml in 0.15 M NaCl with 2.0 kGy gamma radiation emitted by a 60Co source. The native and irradiated toxins...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotamine; Gamma radiation; Chromatography; Toxicity; Circular dichroism.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200004

Alterations in proteins of bone marrow extracellular matrix in undernourished mice
Provedor de dados: 56
Autores: Vituri,C.L.; Alvarez-Silva,M.; Trentin,A.G.; Borelli,P.
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein as compared to 20% casein in the control diet. When the experimental group had attained a 20% loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5%) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibronectin; Bone marrow; Extracellular matrix; Malnutrition.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800004

Cardiovascular responses to microinjections of GABA or anesthetics into the rostral ventrolateral medulla of conscious and anesthetized rats
Provedor de dados: 56
Autores: Lacerda,J.E.C.; Campos,R.R.; Araujo,G.C.; Andreatta-Van Leyen,S.; Lopes,O.U.; Guertzenstein,P.G.
The rostral ventrolateral medulla (RVLM) contains neurons involved in tonic and reflex control of arterial pressure. We describe the effects of gamma-aminobutyric acid (GABA) and anesthetics injected into the RVLM of conscious and urethane (1.2 g/kg, iv) anesthetized Wistar rats (300-350 g). In conscious rats, bilateral microinjection of GABA (50 nmol/200 nl) induced a small but significant decrease in blood pressure (from 130 ± 3.6 to 110 ± 5.6 mmHg, N = 7). A similar response was observed with sodium pentobarbital microinjection (24 nmol/200 nl). However, in the same animals, the fall in blood pressure induced by GABA (from 121 ± 8.9 to 76 ± 8.8 mmHg, N = 7) or pentobarbital (from 118 ± 4.5 to 57 ± 11.3 mmHg, N = 6) was significantly increased after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasomotor tone; Ventrolateral medulla; RVLM; GABA; Conscious rats.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900019

Use of the caffeine-halothane contracture test for the diagnosis of malignant hyperthermia in Brazil
Provedor de dados: 56
Autores: Sudo,R.T.; Cunha,L.B.P.; Carmo,P.L.; Matos,A.R.; Trachez,M.M.; Cardoso,L.A.M.; Aguiar,M.I.S.; Abreu,A.V.; Zapata-Sudo,G.
Malignant hyperthermia (MH) is a pharmacogenetic disease triggered by volatile anesthetics and succinylcholine. Deaths due to MH have been reported in Brazil. The first Malignant Hyperthermia Diagnostic and Research Center in Latin America was inaugurated in 1993 at the Federal University of Rio de Janeiro, Brazil. The center followed the diagnostic protocols of the North America MH Group, in which the contractures of biopsies from the vastus lateralis muscle are analyzed after exposure to caffeine and halothane (CHCT). CHCT was performed in individuals who survived, their relatives and those with signs/symptoms somewhat related to MH susceptibility (MHS). Here, we report data from 194 patients collected over 16 years. The Southeast (N = 110) and South (N...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant hyperthermia; Diagnosis; Skeletal muscle; Anesthesia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600005

The role of the vagus nerve in hypertonic resuscitation of hemorrhagic shocked dogs
Provedor de dados: 56
Autores: Velasco,I.T.; Baena,R.C.
Previous studies have suggested a critical role for the vagi during the hypertonic resuscitation of hemorrhagic shocked dogs. Vagal blockade prevented the full hemodynamic and metabolic recovery and increased mortality. This interpretation, however, was challenged on the grounds that the blockade also abolished critical compensatory mechanisms and therefore the animals would die regardless of treatment. To test this hypothesis, 29 dogs were bled (46.0 ± 6.2 ml/kg, enough to reduce the mean arterial pressure to 40 mmHg) and held hypotensive for 45 min. After 40 min, vagal activity was blocked in a reversible manner (0ºC/15 min) and animals were resuscitated with 7.5% NaCl (4 ml/kg), 0.9% NaCl (32 ml/kg), or the total volume of shed blood. In the vagal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertonic resuscitation; Hypertonic sodium chloride; Hemorrhagic shock; Plasma volume.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300020

Effects of centrally acting antihypertensive drugs on the microcirculation of spontaneously hypertensive rats
Provedor de dados: 56
Autores: Estato,V.; Araújo,C.V.; Bousquet,P.; Tibiriçá,E.
We investigated the acute effects of centrally acting antihypertensive drugs on the microcirculation of pentobarbital-anesthetized spontaneously hypertensive rats (SHR). The effects of the sympatho-inhibitory agents clonidine and rilmenidine, known to activate both alpha2-adrenoceptors and nonadrenergic I1-imidazoline binding sites (I1BS) in the central nervous system, were compared to those of dicyclopropylmethyl-(4,5-dimethyl-4,5-dihydro-3H -pyrrol-2-yl)-amine hydrochloride (LNP 509), which selectively binds to the I1BS. Terminal mesenteric arterioles were observed by intravital microscopy. Activation of the central sympathetic system with L-glutamate (125 µg, ic) induced marked vasoconstriction of the mesenteric microcirculation (27 ± 3%; N = 6, P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenteric microcirculation; Arterial hypertension; Clonidine; Rilmenidine; Baclofen; LNP 509.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000014

Inflammatory and oxidative stress airway markers in premature newborns of hypertensive mothers
Provedor de dados: 56
Autores: Madoglio,R.J.; Rugolo,L.M.S.S.; Kurokawa,C.S.; Sá,M.P.A.; Lyra,J.C.; Antunes,L.C.O.
Although oxidative stress and inflammation are important mechanisms in the pathophysiology of preeclampsia and preterm diseases, their contribution to the respiratory prognosis of premature infants of hypertensive mothers is not known. Our objective was to determine the levels of oxidative stress and inflammation markers in the airways of premature infants born to hypertensive and normotensive mothers, in the first 72 h of life, and to investigate whether they are predictors of bronchopulmonary dysplasia (BPD)/death. This was a prospective study with premature infants less than 34 weeks’ gestation on respiratory support who were stratified into 2 groups: 32 premature infants of hypertensive mothers and 41 of normotensive women, with a mean gestational age...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infant; Premature; Pre-eclampsia; Oxidative stress; Cytokines; Bronchopulmonary dysplasia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900703

Partial characterization of nif genes from the bacterium Azospirillum amazonense
Provedor de dados: 56
Autores: Potrich,D.P.; Passaglia,L.M.P.; Schrank,I.S.
Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azospirillum amazonense; Nif genes; GlnB; 16S rDNA.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900002

Extracts of Ascaris suum egg and adult worm share similar immunosuppressive properties
Provedor de dados: 56
Autores: Souza,V.M.O.; Faquim-Mauro,E.L.; Macedo,M.S.
Adult Ascaris suum body extract (Asc) prepared from male and female worms (with stored eggs) down-regulates the specific immune response of DBA/2 mice to ovalbumin (OA) and preferentially stimulates a Th2 response to its own components, which is responsible for the suppression of the OA-specific Th1 response. Here, we investigated the participation of soluble extracts prepared from male or female worms or from eggs (E-Asc) in these immunological events. Extracts from either sex (1 mg/animal) or E-Asc (0.35 or 1 mg protein/animal) suppressed the delayed-type hypersensitivity (DTH) reaction (60-85%), proliferative response (50-70%), IL-2 and IFN-gamma secretion (below detection threshold) and IgG1 antibody production (70-90%) of DBA/2 mice to OA. A dose of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascaris suum; Immunosuppression; Adult worms; Eggs; Cytokines.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100012

Sleep, ageing and night work
Provedor de dados: 56
Autores: Pires,M.L.N.; Teixeira,C.W.; Esteves,A.M.; Bittencourt,L.R.A.; Silva,R.S.; Santos,R.F.; Tufik,S.; Mello,M.T.
Studies have shown that the frequency or worsening of sleep disorders tends to increase with age and that the ability to perform circadian adjustments tends to decrease in individuals who work the night shift. This condition can cause consequences such as excessive sleepiness, which are often a factor in accidents that occur at work. The present study investigated the effects of age on the daytime and nighttime sleep patterns using polysomnography (PSG) of long-haul bus drivers working fixed night or day shifts. A total of 124 drivers, free of sleep disorders and grouped according to age (&lt;45 years, N = 85, and ≥45 years, N = 39) and PSG timing (daytime (D) PSG, N = 60; nighttime (N) PSG, N = 64) participated in the study. We observed a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep; Shift work; Night work; Aging; Polysomnography; REM; Slow wave sleep (SWS).
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900011

Intestinal permeability in strongyloidiasis
Provedor de dados: 56
Autores: Werneck-Silva,A.L.; Sipahi,A.M.; Damião,A.O.M.C.; Buchpigue,C.A.; Iriya,K.; Laudanna,A.A.
The objective of the present study was to assess intestinal permeability in patients with infection caused by Strongyloides stercoralis. Twenty-six patients (16 women and 10 men), mean age 45.9, with a diagnosis of strongyloidiasis were evaluated. For comparison, 25 healthy volunteers (18 women and 7 men), mean age 44.9, without digestive disorders or intestinal parasites served as normal controls. Intestinal permeability was measured on the basis of urinary radioactivity levels during the 24 h following oral administration of chromium-labeled ethylenediaminetetraacetic acid (51Cr-EDTA) expressed as percentage of the ingested dose. The urinary excretion of 51Cr-EDTA was significantly reduced in patients with strongyloidiasis compared to controls (1.60 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal permeability; 51Cr-EDTA; Strongyloidiasis; Small bowel.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300009

The use of light-emitting diodes to stimulate mitochondrial function and liver regeneration of partially hepatectomized rats
Provedor de dados: 56
Autores: Castro-e-Silva,T.; Castro-e-Silva,O.; Kurachi,C.; Ferreira,J.; Zucoloto,S.; Bagnato,V.S.
The biostimulating effect of laser radiation has been observed in many areas of Medicine. However, there are still several questions to be answered, among them the importance of light coherence in the stimulatory process. In the present study, we used light-emitting diodes (LED) to promote the stimulation of liver regeneration after partial hepatectomy in rats. Fourteen male Wistar rats weighing 200-250 g were submitted to partial hepatectomy (70%) followed by LED light irradiation (630 nm) of the remaining part of the liver at two doses, i.e., 10 (N = 7) and 140 (N = 7) J/cm². A group irradiated with laser, 590 nm (N = 7, 15 J/cm²) was performed for the study of proliferating cell nuclear antigen-labeling index. Data are reported as mean ± SEM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver; Mitochondria; Laser; Light-emitting diode; Liver regeneration; Proliferating cell nuclear antigen.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800006

Computerized analysis of isometric tension studies provides important additional information about vasomotor activity
Provedor de dados: 56
Autores: Vincent,M.B.; White,L.R.; Bakken,I.J.; Sjaavaag,I.
Concentration-response curves of isometric tension studies on isolated blood vessels are obtained traditionally. Although parameters such as Imax, EC50 and pA2 may be readily calculated, this method does not provide information on the temporal profile of the responses or the actual nature of the reaction curves. Computerized data acquisition systems can be used to obtain average data that represent a new source of otherwise inaccessible information, since early and late responses may be observed separately in detail
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Vasoactivity; Digitalization; Substance P; Acetylcholine; VIP; CGRP.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000015

Adverse pregnancy outcome in rats following exposure to a Salacia reticulata (Celastraceae) root extract
Provedor de dados: 56
Autores: Ratnasooriya,W.D.; Jayakody,J.R.A.C.; Premakumara,G.A.S.
The root extract of Salacia reticulata Wight (family: Celastraceae) is used in Sri Lanka by traditional practitioners as a herbal therapy for glycemic control even during pregnancy. It is recognized that some clinically used antidiabetic drugs have harmful effects on pregnancy but the effects of the S. reticulata root extract on reproductive outcome is unknown and deserves examination. We determined the effects of the S. reticulata root extract on the reproductive outcome of Wistar rats (250-260 g) when administered orally (10 g/kg) during early (days 1-7) and mid- (days 7-14) pregnancy. The root extract significantly (P&lt;0.05) enhanced post-implantation losses (control vs treatment: early pregnancy, 4.7 ± 2.4 vs 49.3 ± 13%; mid-pregnancy, 4.7 ± 2.4 vs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salacia reticulata; Hypoglycemia; Pregnancy outcome; Birth weight; Postnatal development.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700015

Bone metabolism and vascular calcification
Provedor de dados: 56
Autores: Danilevicius,C.F.; Lopes,J.B.; Pereira,R.M.R.
Osteoporosis and atherosclerosis are chronic degenerative diseases which have been considered to be independent and whose common characteristic is increasing incidence with age. At present, growing evidence indicates the existence of a correlation between cardiovascular disease and osteoporosis, irrespective of age. The morbidity and mortality of osteoporosis is mainly related to the occurrence of fractures. Atherosclerosis shows a high rate of morbidity and especially mortality because of its clinical repercussions such as angina pectoris, acute myocardial infarction, stroke, and peripheral vascular insufficiency. Atherosclerotic disease is characterized by the accumulation of lipid material in the arterial wall resulting from autoimmune and inflammatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Vascular calcification; Atherosclerosis; Low-bone mineral density; Arterial calcification.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400001

Effects of lipopolysaccharide on low- and high-density cultured rabbit vascular smooth muscle cells: differential modulation of nitric oxide release, ERK1/ERK2 MAP kinase activity, protein tyrosine phosphatase activity, and DNA synthesis
Provedor de dados: 56
Autores: Barbosa de Oliveira,L.C.; Rocha Oliveira,C.J.; Fries,D.M.; Stern,A.; Monteiro,H.P.
Previous studies have shown that exogenously generated nitric oxide (NO) inhibits smooth muscle cell proliferation. In the present study, we stimulated rabbit vascular smooth muscle cells (RVSMC) with E. coli lipopolysaccharide (LPS), a known inducer of NO synthase transcription, and established a connection between endogenous NO, phosphorylation/dephosphorylation-mediated signaling pathways, and DNA synthesis. Non-confluent RVSMC were cultured with 0, 5, 10, or 100 ng/ml of the endotoxin. NO release was increased by 86.6% (maximum effect) in low-density cell cultures stimulated with 10 ng/ml LPS as compared to non-stimulated controls. Conversely, LPS (5 to 100 ng/ml) did not lead to enhanced NO production in multilayered (high density) RVSMC. DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Lipopolysaccharide; Protein tyrosine phosphatases; Mitogen-activated protein kinases; DNA synthesis; Cell density.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200006

Comparison of AutoSetä and polysomnography for the detection of apnea-hypopnea events
Provedor de dados: 56
Autores: Bagnato,M.C.; Nery,L.E.; Moura,S.M.T.; Bittencourt,L.R.A.; Tufik,S.
The use of the flow vs time relationship obtained with the nasal prongs of the AutoSetä (AS) system (diagnosis mode) has been proposed to detect apneas and hypopneas in patients with reasonable nasal patency. Our aim was to compare the accuracy of AS to that of a computerized polysomnographic (PSG) system. The study was conducted on 56 individuals (45 men) with clinical characteristics of obstructive sleep apnea (OSA). Their mean (± SD) age was 44.6 ± 12 years and their body mass index was 31.3 ± 7 kg/m2. Data were submitted to parametric analysis to determine the agreement between methods and the intraclass correlation coefficient was calculated. The Student t-test and Bland and Altman plots were also used. Twelve patients had an apnea-hypopnea index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obstructive sleep apnea; Polysomnography; Computerized diagnostic system.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500005

Transient high-level expression of ß-galactosidase after transfection of fibroblasts from GM1 gangliosidosis patients with plasmid DNA
Provedor de dados: 56
Autores: Balestrin,R.C.; Baldo,G.; Vieira,M.B.; Sano,R.; Coelho,J.C.; Giugliani,R.; Matte,U.
GM1 gangliosidosis is an autosomal recessive disorder caused by the deficiency of lysosomal acid hydrolase ß-galactosidase (ß-Gal). It is one of the most frequent lysosomal storage disorders in Brazil, with an estimated frequency of 1:17,000. The enzyme is secreted and can be captured by deficient cells and targeted to the lysosomes. There is no effective treatment for GM1 gangliosidosis. To determine the efficiency of an expression vector for correcting the genetic defect of GM1 gangliosidosis, we tested transfer of the ß-Gal gene (Glb1) to fibroblasts in culture using liposomes. ß-Gal cDNA was cloned into the expression vectors pSCTOP and pREP9. Transfection was performed using 4 µL lipofectamine 2000 and 1.5-2.0 µg DNA. Cells (2 x 10(5)/well) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GM1 gangliosidosis; SS-galactosidase deficiency; Gene therapy; Lysosomal storage disorder; Lipofectamine.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400005

Effect of obesity on rat reproduction and on the development of their adult offspring
Provedor de dados: 56
Autores: Campos,K.E.; Volpato,G.T.; Calderon,I.M.P.; Rudge,M.V.C.; Damasceno,D.C.
The aim of the present study was to assess the reproductive parameters of obese Wistar rats and to determine the frequency of their obese adult offspring. Neonatal rats were divided into two groups: F1 generation, induced to obesity by monosodium glutamate (MSG; F1MSG, N = 30), and rats given saline (F1CON, N = 13). At 90 days of age all animals were mated, producing the F2 offspring (F2CON, N = 28; F2MSG, N = 15). Reproductive parameters (fertility, pregnancy, and delivery indexes) were evaluated in F1 rats. F2 newborns were weighed, and the obesity parameter for F1 and F2 generations was determined from months 5 to 7 of life. At month 7, periovarian fat was weighed and no differences were found. Mean newborn weight also did not differ. The F1 and F2MSG...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Obesity; Offspring; Fertility; Wistar rats; Monosodium glutamate; Transgeneration.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200008

Diagnostic and prognostic value of procalcitonin for early intracranial infection after craniotomy
Provedor de dados: 56
Autores: Yu,Y.; Li,H.J.
Intracranial infection is a common clinical complication after craniotomy. We aimed to explore the diagnostic and prognostic value of dynamic changing procalcitonin (PCT) in early intracranial infection after craniotomy. A prospective study was performed on 93 patients suspected of intracranial infection after craniotomy. Routine peripheral venous blood was collected on the day of admission, and C reactive protein (CRP) and PCT levels were measured. Cerebrospinal fluid (CSF) was collected for routine biochemical, PCT and culture assessment. Serum and CSF analysis continued on days 1, 2, 3, 5, 7, 9, and 11. The patients were divided into intracranial infection group and non-intracranial infection group; intracranial infection group was further divided into...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Intracranial infection; Procalcitonin; Cerebrospinal fluid procalcitonin; Craniotomy; Diagnosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500608

Extremely elevated IL-18 levels may help distinguish systemic-onset juvenile idiopathic arthritis from other febrile diseases
Provedor de dados: 56
Autores: Xia,Y.; Cui,P.; Li,Q.; Liang,F.; Li,C.; Yang,J.
The aim of this research was to explore whether IL-18 can be a serological marker for the diagnosis of systemic-onset juvenile idiopathic arthritis (sJIA). A total of 23 sJIA patients (13 males, median age 8.2), 20 acute lymphoblastic leukemia (ALL) patients, 18 patients with severe infections (SIF), 26 Kawasaki disease (KD) patients, 18 juvenile idiopathic arthritis (JIA) patients, and 25 healthy control patients were selected for this study. Enzyme-linked immunosorbent assays (ELISAs) were used to determine the serum concentrations of the S100A8, S100A9, and IL-6 proteins. The serum IL-18 levels were detected by a cytometric bead array (CBA). The serum IL-6 concentrations in various disease groups were significantly higher than that in the healthy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic-onset juvenile idiopathic arthritis (sJIA); Serological markers; IL-18; S100A8/S100A9.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200703

Dyspnea perception in cystic fibrosis patients
Provedor de dados: 56
Autores: Ziegler,B.; Fernandes,A.K.; Sanches,P.R.S.; Junior,D.P. Silva; Thomé,P.R.O.; Dalcin,P.T.R.
We evaluated dyspnea perception in cystic fibrosis patients compared with normal subjects, during an inspiratory resistive loading test and 6-min walk test. We also evaluated the correlation between dyspnea scores induced by resistive loads and by the 6-min walk test. In this prospective, cross-sectional study, 31 patients with cystic fibrosis (≥15 years of age) and 31 age-, gender-, and ethnicity-matched healthy volunteers (20 females and 11 males per group) underwent inspiratory resistive loading, spirometry, and the 6-min walk test. As the magnitude of the inspiratory loads increased, dyspnea scores increased (P&lt;0.001), but there was no difference between groups in dyspnea score (P=0.654). Twenty-six (84%) normal subjects completed all the resistive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic fibrosis; Dyspnea perception; Pulmonary function test; Six-min walk test; Inspiratory resistive load testing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000897

Dynamic resistance training decreases sympathetic tone in hypertensive ovariectomized rats
Provedor de dados: 56
Autores: Shimojo,G.L.; Palma,R.K.; Brito,J.O.; Sanches,I.C.; Irigoyen,M.C.; De Angelis,K.
The aim of this study was to investigate the effects of resistance exercise training on hemodynamics and cardiac autonomic control in ovariectomized spontaneously hypertensive rats. Female rats were divided into 4 groups: sedentary control (SC), sedentary hypertensive (SH), sedentary hypertensive ovariectomized (SHO), and resistance-trained hypertensive ovariectomized (RTHO). Resistance exercise training was performed on a vertical ladder (5 days/week, 8 weeks) at 40-60% maximal load. Direct arterial pressure was recorded. Vagal and sympathetic tones were measured by heart rate (HR) responses to methylatropine (3 mg/kg, iv) and propranolol (4 mg/kg, iv). Ovariectomy resulted in additional increases in blood pressure in hypertensive rats and was associated...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Resistance exercise training; Menopause; Hypertension; Sympathetic tone; Blood pressure.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600523

Non-neuronal cells are not the limiting factor for the low axonal regeneration in C57BL/6J mice
Provedor de dados: 56
Autores: Oliveira,A.L.R.; Langone,F.
Peripheral axonal regeneration was investigated in adult male mice of the C57BL/6J (C), BALB/cJ (B) and A/J (A) strains and in their F1 descendants using a predegenerated nerve transplantation model. Four types of transplants were performed: 1) isotransplants between animals of the C, B and A strains; 2) donors of the C strain and recipients of the C x B and C x A breeding; 3) donors of the B strain and recipients of the C x B breeding, and 4) donors of the A strain and recipients of the C x A breeding. Donors had the left sciatic nerve transected and two weeks later a segment of the distal stump was transplanted into the recipient. Four weeks after transplantation the regenerated nerves were used to determine the total number of regenerated myelinated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nerve regeneration; Schwann cells; Isogenic mice; Nerve transplantation; Tubulization.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200011

Treatment of cytomegalovirus retinitis with an intraocular sustained-release ganciclovir implant
Provedor de dados: 56
Autores: Muccioli,C.; Belfort Jr.,R.
The objective of this prospective study was to evaluate the efficacy and complications of the use of an intraocular sustained-release ganciclovir implant for the treatment of active cytomegalovirus (CMV) retinitis in AIDS patients. Thirty-nine eyes of 26 patients were submitted to ocular surgery. All patients underwent complete ocular examination before and after surgery. The surgical procedure was always done under local anesthesia using the same technique. The mean time for the surgical procedure was 20 min (range, 15 to 30 min). The average follow-up period was 3.7 months. Of all patient, only 4 presented recurrence of retinitis after 8, 8, 9 and 2 months, respectively. Three of them received a successful second implant. All 39 eyes of the 26 patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus; Retinitis; Intraocular inflammation; AIDS; Uveitis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700008

Abnormal response of left ventricular systolic function to submaximal exercise in post-partial left ventriculotomy patients
Provedor de dados: 56
Autores: Herdy,A.H.; Moritz,P.; Assis,A.V.; Ribeiro,F.; Collaço,J.; Ribeiro,J.P.
Patients with heart failure who have undergone partial left ventriculotomy improve resting left ventricular systolic function, but have limited functional capacity. We studied systolic and diastolic left ventricular function at rest and during submaximal exercise in patients with previous partial left ventriculotomy and in patients with heart failure who had not been operated, matched for maximal and submaximal exercise capacity. Nine patients with heart failure previously submitted to partial left ventriculotomy were compared with 9 patients with heart failure who had not been operated. All patients performed a cardiopulmonary exercise test with measurement of peak oxygen uptake and anaerobic threshold. Radionuclide left ventriculography was performed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Radionuclide ventriculography; Anaerobic threshold; Left ventricular diastolic function; Left ventricular ejection fraction.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200002

High prevalence of the GSTM3*A/B polymorphism in sub-Sarahan African populations
Provedor de dados: 56
Autores: Teixeira,D.; Vargens,D.; Príncipe,A.; Oliveira,E.; Amorim,A.; Prata,M.J.; Suarez-Kurtz,G.
A 3-bp insertion/deletion polymorphism in intron 6 of GSTM3 (rs1799735, GSTM3*A/*B) affects the activity of the phase 2 xenobiotic metabolizing enzyme GSTM3 and has been associated with increased cancer risk. The GSTM3*B allele is rare or absent in Southeast Asians, occurs in 5-20% of Europeans but was detected in 80% of Bantu from South Africa. The wide genetic diversity among Africans led us to investigate whether the high frequency of GSTM3*B prevailed in other sub-Saharan African populations. In 168 healthy individuals from Angola, Mozambique and the São Tomé e Príncipe islands, the GSTM3*B allele was three times more frequent (0.74-0.78) than the GSTM3*A allele (0.22-0.26), with no significant differences in allele frequency across the three groups....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African populations; GSTM3 polymorphisms; Multidimensional scaling analysis; Pharmacogenetics; Population diversity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700010

Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
Provedor de dados: 56
Autores: Verbisck,N.V.; Da-Silva,S.; Mortara,R.A.
We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Amastigote; Monoclonal antibodies; Surface antigen; Polymorphism.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200011

Effect of L-arginine, dimercaptosuccinic acid (DMSA) and the association of L-arginine and DMSA on tissue lead mobilization and blood pressure level in plumbism
Provedor de dados: 56
Autores: Malvezzi,C.K.; Moreira,E.G.; Vassilieff,I.; Vassilieff,V.S.; Cordellini,S.
Lead (Pb)-induced hypertension is characterized by an increase in reactive oxygen species (ROS) and a decrease in nitric oxide (NO). In the present study we evaluated the effect of L-arginine (NO precursor), dimercaptosuccinic acid (DMSA, a chelating agent and ROS scavenger), and the association of L-arginine/DMSA on tissue Pb mobilization and blood pressure levels in plumbism. Tissue Pb levels and blood pressure evolution were evaluated in rats exposed to: 1) Pb (750 ppm, in drinking water, for 70 days), 2) Pb plus water for 30 more days, 3) Pb plus DMSA (50 mg kg-1 day-1, po), L-arginine (0.6%, in drinking water), and the combination of L-arginine/DMSA for 30 more days, and 4) their respective matching controls. Pb exposure increased Pb levels in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: L-arginine; DMSA; Tissue lead mobilization; Plumbism; Blood pressure; Rat.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000016

Cardiovascular and autonomic modulation by the central nervous system after aerobic exercise training
Provedor de dados: 56
Autores: Martins-Pinge,M.C.
The autonomic nervous system plays a key role in maintaining homeostasis under normal and pathological conditions. The sympathetic tone, particularly for the cardiovascular system, is generated by sympathetic discharges originating in specific areas of the brainstem. Aerobic exercise training promotes several cardiovascular adjustments that are influenced by the central areas involved in the output of the autonomic nervous system. In this review, we emphasize the studies that investigate aerobic exercise training protocols to identify the cardiovascular adaptations that may be the result of central nervous system plasticity due to chronic exercise. The focus of our study is on some groups of neurons involved in sympathetic regulation. They include the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brainstem; Hypothalamus; Aerobic exercise training; Rostroventrolateral medulla; Paraventricular nucleus; Nucleus tractus solitarii.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900004

Lead (Pb2+) and cadmium (Cd2+) inhibit the dipsogenic action of central beta-adrenergic stimulation by isoproterenol
Provedor de dados: 56
We have previously demonstrated that acute third ventricle injections of both Pb2+ and Cd2+ impair the dipsogenic response elicited by three different situations: dehydration and central cholinergic or angiotensinergic stimulation. ß-Adrenergic activation is part of the multifactorial integrated systems operating in drinking behavior control in the central nervous system. In the present study acute third ventricle injections of Pb2+ (3, 30 and 300 pmol/rat) or Cd2+ (0.3, 3 and 30 pmol/rat) blocked the dipsogenic response induced by third ventricle injections of isoproterenol (ISO; 160 nmol/rat) in a dose-dependent manner. Normohydrated animals receiving ISO + NaAc (sodium acetate) or saline (controls) displayed a high water intake after 120 min (ISO +...
Palavras-chave: Lead; Cadmium; Water intake; Isoproterenol.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300018

IFN-g in human Chagas' disease: protection or pathology?
Provedor de dados: 56
Autores: Bahia-Oliveira,L.M.G.; Gomes,J.A.S.; Rocha,M.O.C.; Moreira,M.C.V.; Lemos,E.M.; Luz,Z.M.P.; Pereira,M.E.S.; Coffman,R.L.; Dias,J.C.P.; Cançado,J.R.; Gazzinelli,G.; Corrêa-Oliveira,R.
An apparently paradoxical role for IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; in human Chagas' disease was observed when studying the pattern of cytokine production by peripheral blood mononuclear cells (PBMC) obtained from two groups of chagasic patients after specific stimulation with Trypanosoma cruzi-derived antigens. The groups studied were 1) patients treated with benznidazole during the acute phase of Trypanosoma cruzi infection and 2) chronically infected untreated patients. In the treated group, higher levels of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; were produced by PBMC from individuals cured after treatment when compared to non-cured patients. In contrast, in the chronically infected group (not treated) higher levels of IFN-&lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi Chagas' disease cardiomyopathy peripheral blood mononuclear cells (PBMC) cytokines INF-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100017

Involvement of the caudal raphe nuclei in the feeding behavior of rats
Provedor de dados: 56
Autores: Takase,L.F.; Barone,J.R.; Nogueira,M.I.
Involvement of the caudal raphe nuclei (raphe pallidus, RPa; raphe magnus, RMg, and raphe obscurus, ROb) in feeding behavior of adult rats was studied by measuring c-Fos protein expression, in animals submitted to the "meal-feeding" model of food restriction in which the rats were fed ad libitum only from 7:00 to 9:00 h, for 15 days. The experimental groups submitted to chronic fasting, named 'search for food' (SF), 'ingestion of food' (IF) and 'satiety of food' (SaF) were scheduled after a previous study in which the body weight and the general and feeding behaviors were evaluated by daily monitoring. Acute, 48-h fasting (AF) was used as control. In the chronic group, the animals presented a 16% reduction in body weight in the first week, followed by a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Feeding behavior; Caudal raphe nuclei; C-Fos protein expression; Meal-feeding.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200010

Rotavirus and reovirus interaction with mouse peritoneal resident phagocytic cells
Provedor de dados: 56
Autores: Guimarães,M.A.A.M.; Nozawa,C.M.; Guimarães,A.C.C.; Ramos,S.
Rotaviruses and reoviruses are involved in human and animal diseases. It is known that both viruses penetrate the gastrointestinal tract but their interaction with phagocytic cells is unknown. To study this interaction, peritoneal resident phagocytic cells were used and rotavirus and reovirus replication in peritoneal phagocytic cells was observed. However, rotavirus replication in these cells led to the production of defective particles since MA-104 cells inoculated with rotavirus phagocytic cell lysate did not show any evidence of virus replication. On the basis of these results, we suggest that, although reovirus dissemination may be helped by these phagocytic cells, these cells may control rotavirus infection and probably contribute to the prevention...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Reovirus; Phagocytic cells.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000008

Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos
Provedor de dados: 56
Autores: de Souza,D.K.; Salles,L.P.; Rosa e Silva,A.A.M.
Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo metabolism in vitro and in vivo, and discuss the use of metabolic analysis of embryos produced in vitro to assess viability and predict a viable pregnancy after transference to the female tract.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine pregnancy rates; Embryo metabolism; Metabolism profile; In vitro fertilization; Embryo culture conditions; In vivo embryo.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300191

Utility of a novel risk score for prediction of ventricular tachycardia and cardiac death in chronic Chagas disease - the SEARCH-RIO study
Provedor de dados: 56
Autores: Benchimol-Barbosa,P.R.; Tura,B.R.; Barbosa,E.C.; Kantharia,B.K.
The SEARCH-RIO study prospectively investigated electrocardiogram (ECG)-derived variables in chronic Chagas disease (CCD) as predictors of cardiac death and new onset ventricular tachycardia (VT). Cardiac arrhythmia is a major cause of death in CCD, and electrical markers may play a significant role in risk stratification. One hundred clinically stable outpatients with CCD were enrolled in this study. They initially underwent a 12-lead resting ECG, signal-averaged ECG, and 24-h ambulatory ECG. Abnormal Q-waves, filtered QRS duration, intraventricular electrical transients (IVET), 24-h standard deviation of normal RR intervals (SDNN), and VT were assessed. Echocardiograms assessed left ventricular ejection fraction. Predictors of cardiac death and new onset...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas heart disease; Noninvasive electrocardiography; Prognostic score; Cardiac death; Ventricular tachycardia.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100974

Relationship between deep venous thrombosis and inflammatory cytokines in postoperative patients with malignant abdominal tumors
Provedor de dados: 56
Autores: Du,T.; Tan,Z.
Deep venous thrombosis (DVT) is a common surgical complication in cancer patients and evidence that inflammation plays a role in the occurrence of DVT is increasing. We studied a population of cancer patients with abdominal malignancies with the aim of investigating whether the levels of circulating inflammatory cytokines were associated with postoperative DVT, and to determine the levels in DVT diagnoses. The serum levels of C-reactive protein (CRP), interleukins (IL)-6 and IL-10, nuclear transcription factor-κB (NF-κB) and E-selectin (E-Sel) were determined in 120 individuals, who were divided into 3 groups: healthy controls, patients with and patients without DVT after surgery for an abdominal malignancy. Data were analyzed by ANOVA, Dunnet's T3 test,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant tumor; Abdominal surgery; Deep venous thrombosis (DVT); Inflammatory cytokine; Correlation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001101003

Exosomes promote cetuximab resistance via the PTEN/Akt pathway in colon cancer cells
Provedor de dados: 56
Autores: Zhang,S.; Zhang,Y.; Qu,J.; Che,X.; Fan,Y.; Hou,K.; Guo,T.; Deng,G.; Song,N.; Li,C.; Wan,X.; Qu,X.; Liu,Y.
Cetuximab is widely used in patients with metastatic colon cancer expressing wildtype KRAS. However, acquired drug resistance limits its clinical efficacy. Exosomes are nanosized vesicles secreted by various cell types. Tumor cell-derived exosomes participate in many biological processes, including tumor invasion, metastasis, and drug resistance. In this study, exosomes derived from cetuximab-resistant RKO colon cancer cells induced cetuximab resistance in cetuximab-sensitive Caco-2 cells. Meanwhile, exosomes from RKO and Caco-2 cells showed different levels of phosphatase and tensin homolog (PTEN) and phosphor-Akt. Furthermore, reduced PTEN and increased phosphorylated Akt levels were found in Caco-2 cells after exposure to RKO cell-derived exosomes....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cetuximab; Exosome; PTEN; Akt; Colon cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100609

A comparative study of aging of the elastic fiber system of the diaphragm and the rectus abdominis muscles in rats
Provedor de dados: 56
Autores: Rodrigues,C.J.; Rodrigues Junior,A.J.
In the present study the age-related changes of the striated muscle elastic fiber system were investigated in the diaphragm and rectus abdominis muscles of 1-, 4-, 8- and 18-month-old rats. The activation patterns of these muscles differ in that the diaphragm is regularly mobilized tens of times every minute during the entire life of the animal whereas the rectus abdominis, although mobilized in respiration, is much less and more irregularly activated. The elastic fibers were stained by the Verhoeff technique for mature elastic fibers. Weigert stain was used to stain mature and elaunin elastic fibers, and Weigert-oxone to stain mature, elaunin and oxytalan elastic fibers. The density of mature and elaunin elastic fibers showed a progressive increase with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Elastin; Extracellular matrix; Diaphragm; Rectus abdominis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200008

Enhanced mucosal re-epithelialization induced by short chain fatty acids in experimental colitis
Provedor de dados: 56
Autores: Aguilar-Nascimento,J.E.; França-da-Silva,L.R.; de-Oliveira,A.F.; Gomes-da-Silva,M.H.
The short chain fatty acids (SCFA) are the best nutrients for the colonocytes. Glucose is poorly used as a fuel but may be transformed into SCFA by colonic bacteria. The aim of this study was to investigate the effect of SCFA or glucose on experimental colitis. Colitis was induced in 30 Wistar rats by colonic instillation of 4% acetic acid. Five days later they were randomized to receive twice a day colonic lavage containing saline (controls, N = 10), 10% hypertonic glucose (N = 10) or SCFA (N = 10) until day 8 when they were killed. At autopsy, the colon was removed and weighed and the mucosa was evaluated macro- and microscopically and stripped out for DNA assay. Data are reported as mean ± SD or median [range] as appropriate. All animals lost weight but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Short chain fatty acids; Ulcerative colitis; Glucose; Colon; Inflammation; Mucosa.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800005

Is persistent hypotension after transient cardiogenic shock associated with an inflammatory response?
Provedor de dados: 56
Autores: Park,M.; A.T.,Maciel; Noritomi,D.T.; Brunialti,M.K.; Salomão,R.; Schettino,G.P.P.; Azevedo,L.C.P.
We evaluated the recovery of cardiovascular function after transient cardiogenic shock. Cardiac tamponade was performed for 1 h and post-shock data were collected in 5 domestic large white female pigs (43 ± 5 kg) for 6 h. The control group (N = 5) was observed for 6 h after 1 h of resting. During 1 h of cardiac tamponade, experimental animals evolved a low perfusion status with a higher lactate level (8.0 ± 2.2 vs 1.9 ± 0.9 mEq/L), lower standard base excess (-7.3 ± 3.3 vs 2.0 ± 0.9 mEq/L), lower urinary output (0.9 ± 0.9 vs 3.0 ± 1.4 mL·kg-1·h-1), lower mixed venous saturation, higher ileum partial pressure of CO2-end tidal CO2 (EtCO2) gap and a lower cardiac index than the control group. Throughout the 6-h recovery phase after cardiac tamponade,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiogenic shock; Cardiac tamponade; Systemic inflammatory response syndrome; Swine; Myocardial dysfunction; Inflammation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800002

Lung tissue mechanics in the early stages of induced paracoccidioidomycosis in rats
Provedor de dados: 56
Autores: Shikanai-Yasuda,M.A.; Pereira,P.M.; Yamashiro-Kanashiro,E.; Duarte,M.I.S.; Assis,C.M.; Geraldes,E.A.; Saldiva,P.H.N.
Pulmonary dysfunction represents the most important cause of death in patients with paracoccidioidomycosis (PBM). In order to investigate the functional changes of the lungs in the early stages of PBM, a model of benign disease was developed by intratracheal challenge of 12-week old isogenic Wistar rats with 1 x 106 yeast forms of Paracoccidioides brasiliensis. Animals were studied 30 and 60 days after infection, when fully developed granulomas were demonstrable in the lungs. Measurements of airway resistance, lung elastance and tissue hysteresis were made during sinusoidal deformations (100 breaths/min, tidal volume = 2 ml) with direct measurement of alveolar pressure using the alveolar capsule technique. Infection caused a significant increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental paracoccidioidomycosis; Pulmonary mechanics; Tissue mechanics; Alveolar pressure measurements; Hysteresis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000006

Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats
Provedor de dados: 56
Autores: Gianelo,M.C.S.; Polizzelo,J.C.; Chesca,D.; Mattiello-Sverzut,A.C.
The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Vimentin; Desmin; Intermittent stretching; Collagen.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200603

Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the ProCount™-BD and ISHAGE protocols
Provedor de dados: 56
Autores: Pranke,P.; Hendrikx,J.; Alespeiti,G.; Nardi,N.; Rubinstein,P.; Visser,J.
The total number of CD34+ cells is the most relevant clinical parameter when selecting human umbilical cord blood (HUCB) for transplantation. The objective of the present study was to compare the two most commonly used CD34+ cell quantification methods (ISHAGE protocol and ProCount™ - BD) and analyze the CD34+ bright cells whose 7-amino actinomycin D (7AAD) analysis suggests are apoptotic or dead cells. Twenty-six HUCB samples obtained at the Placental Blood Program of New York Blood Center were evaluated. The absolute numbers of CD34+ cells evaluated by the ISHAGE (with exclusion of 7AAD+ cells) and ProCount™ (with exclusion of CD34+ bright cells) were determined. Using the ISHAGE protocol we found 35.6 ± 19.4 CD34+ cells/µL and with the ProCount™ method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoietic stem cell; Human umbilical cord blood; CD34+; ISHAGE; ProCount.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700008

Genotyping hepatitis C virus from hemodialysis patients in Central Brazil by line probe assay and sequence analysis
Provedor de dados: 56
Autores: Espírito-Santo,M.P.; Carneiro,M.A.S.; Reis,N.R.S.; Kozlowski,A.G.; Teles,S.A.; Lampe,E.; Yoshida,C.F.T.; Martins,R.M.B.
The present study examined the distribution of hepatitis C virus (HCV) genotypes and subtypes in a hemodialysis population in Goiás State, Central Brazil, and evaluated the efficiency of two genotyping methods: line probe assay (LiPA) based on the 5' noncoding region and nucleotide sequencing of the nonstructural 5B (NS5B) region of the genome. A total of 1095 sera were tested for HCV RNA by RT-nested PCR of the 5' noncoding region. The LiPA assay was able to genotype all 131 HCV RNA-positive samples. Genotypes 1 (92.4%) and 3 (7.6%) were found. Subtype 1a (65.7%) was the most prevalent, followed by subtypes 1b (26.7%) and 3a (7.6%). Direct nucleotide sequencing of 340 bp from the NS5B region was performed in 106 samples. The phylogenetic tree showed that...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis C; Hepatitis C virus; Genotypes; Hemodialysis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400013

The decreased oxygen uptake during progressive exercise in ischemia-induced heart failure is due to reduced cardiac output rate
Provedor de dados: 56
Autores: Rolim,N.P.L.; Mattos,K.C.; Brum,P.C.; Baldo,M.V.C.; Middlekauff,H.R.; Negrão,C.E.
We tested the hypothesis that the inability to increase cardiac output during exercise would explain the decreased rate of oxygen uptake (VO2) in recent onset, ischemia-induced heart failure rats. Nine normal control rats and 6 rats with ischemic heart failure were studied. Myocardial infarction was induced by coronary ligation. VO2 was measured during a ramp protocol test on a treadmill using a metabolic mask. Cardiac output was measured with a flow probe placed around the ascending aorta. Left ventricular end-diastolic pressure was higher in ischemic heart failure rats compared with normal control rats (17 ± 0.4 vs 8 ± 0.8 mmHg, P = 0.0001). Resting cardiac index (CI) tended to be lower in ischemic heart failure rats (P = 0.07). Resting heart rate (HR)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Oxygen uptake rate; Cardiac index; Coronary ligation; Ischemia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200018

Comments on the paper "High doses of riboflavin and the elimination of dietary red meat promote the recovery of some motor functions in Parkinson's disease patients. C.G. Coimbraand V.B.C. Junqueira. Brazilian Journal of Medical and Biological Research, 36: 1409-1417, 2003"
Provedor de dados: 56
Autores: Ferraz,H.B.; Quagliato,E.A.B.; Rieder,C.R.M.; Silva,D.J.; Teive,H.A.G.; Barbosa,E.R.; Cardoso,F.; Limongi,J.C.P.; Bezerra,J.M.F.; Andrade,L.A.F.; Allam,N.; Prado,R.C.P.; Tomas,V.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Parkinson's disease; Riboflavin; FAD; Glutathione; Iron; Hemin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900002

Isolation of a ß-galactoside-binding lectin from cat liver
Provedor de dados: 56
Autores: Franco-Fraguas,L.; Batista-Viera,F.; Carlsson,J.
A lectin from cat liver has been identified and purified by affinity chromatography on asialofetuin-Sepharose. One hundred micrograms of lectin was obtained from one cat liver with a purification factor of 1561. The lectin agglutinates trypsin-treated rabbit and cow erythrocytes. Hemagglutination was inhibited only by saccharides containing ß-galactosyl residues, of which the 1-amine-1-deoxy-ß-D-galactose was the most potent one by inhibiting hemagglutination at a concentration of 12.5 mM, followed by melibiose, trehalose and galactose. The lectin has a subunit molecular mass of 14.4 kDa determined by SDS-PAGE under reducing conditions and a pI of 4.85. Compared with the composition of lectins from calf heart and porcine heart, cat liver lectin contains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SS-Galactoside-binding lectin; Galectins; Lectin; Cat liver lectin; Affinity chromatography.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400005

Effect of oral sirolimus therapy on inflammatory biomarkers following coronary stenting
Provedor de dados: 56
Autores: Rosa,W.C.M.; Campos,A.H.; Lima,V.C.
We studied the effect of oral sirolimus, administered to prevent and treat in-stent restenosis (ISR), on the variation of serum levels of inflammatory markers following coronary stenting with bare metal stents. The mean age of the patients was 56 ± 13 years, 65% were males and all had clinically manifested ischemia. Serum levels of high sensitivity C-reactive protein (hs-CRP) concentration were determined by chemiluminescence and serum levels of all other biomarkers by ELISA. One group of patients at high risk for ISR received a loading oral dose of 15 mg sirolimus and 5 mg daily thereafter for 28 days after stenting (SIR-G). A control group (CONT-G) was submitted to stenting without sirolimus therapy. The increase in hs-CRP concentration was highest at 24...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation mediators; Oral sirolimus; Percutaneous coronary intervention; Coronary artery angioplasty; Coronary restenosis; Immunosuppression.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800012

Impact of body characteristics on ultrasound-measured inferior vena cava parameters in Chinese children
Provedor de dados: 56
Autores: Gui,Jianjun; Zhou,Boyang; Liu,Juanhua; Ou,Bing; Wang,Yue; Jiang,Longyuan; Tang,Wanchun; Luo,Baoming; Yang,Zhengfei.
Ultrasound-measured inferior vena cava (IVC) and abdominal aorta (Ao)-associated parameters have been used to predict volume status for decades, yet research focusing on the impact of individual physical characteristics, including gender, height/weight, body surface area (BSA), and age, assessed simultaneously on those parameters in Chinese children is lacking. The aim of the present study was to explore the impact of individual characteristics on maximum IVC diameter (IVCmax), Ao, and IVCmax/Ao in healthy Chinese children. From September to December 2015, 200 healthy children from 1 to 13 years of age were enrolled. IVCmax and Ao diameters were measured by 2D ultrasound. We found that age (years), height (cm), weight (kg), waist circumference (cm), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inferior vena cava; Abdominal aorta; Body characteristics; Volume status.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000601

Nitric oxide synthesis and biological functions of nitric oxide released from ruthenium compounds
Provedor de dados: 56
Autores: Pereira,A.C.; Paulo,M.; Araújo,A.V.; Rodrigues,G.J.; Bendhack,L.M.
During three decades, an enormous number of studies have demonstrated the critical role of nitric oxide (NO) as a second messenger engaged in the activation of many systems including vascular smooth muscle relaxation. The underlying cellular mechanisms involved in vasodilatation are essentially due to soluble guanylyl-cyclase (sGC) modulation in the cytoplasm of vascular smooth cells. sGC activation culminates in cyclic GMP (cGMP) production, which in turn leads to protein kinase G (PKG) activation. NO binds to the sGC heme moiety, thereby activating this enzyme. Activation of the NO-sGC-cGMP-PKG pathway entails Ca2+ signaling reduction and vasodilatation. Endothelium dysfunction leads to decreased production or bioavailability of endogenous NO that could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Vasodilatation; Ruthenium complex; NO-synthase; Resistance artery; Conduit vessel.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900017

Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy
Provedor de dados: 56
Autores: Wang,Z.K.; Yang,L.; Wu,L.L.; Mao,H.; Zhou,Y.H.; Zhang,P.F.; Dai,G.H.
Colon cancer is one of the most common digestive tumors. The present study aimed to explore the functional role, as well as the underlying mechanism of long non-coding RNA LINC00261 in colon cancer. Expression of LINC00261 was analyzed in colon cancer cell lines and human normal cell lines. Acquired resistance cell lines were then built and the acquired resistance efficiency was detected by evaluating cell viability. Thereafter, the effects of LINC00261 overexpression on cisplatin-resistant colon cancer cells were measured, as well as cell apoptosis, viability, migration, and invasion. Subsequently, we investigated the interaction of LINC00261 and β-catenin. The results showed that the LINC00261 gene was down-regulated in colon cancer cell lines and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon cancer; LINC00261; Β-catenin; Cisplatin resistance; Mechanism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200607

New perspective on the pathophysiology of panic: merging serotonin and opioids in the periaqueductal gray
Provedor de dados: 56
Autores: Graeff,F.G.
Panic disorder patients are vulnerable to recurrent panic attacks. Two neurochemical hypotheses have been proposed to explain this susceptibility. The first assumes that panic patients have deficient serotonergic inhibition of neurons localized in the dorsal periaqueductal gray matter of the midbrain that organize defensive reactions to cope with proximal threats and of sympathomotor control areas of the rostral ventrolateral medulla that generate most of the neurovegetative symptoms of the panic attack. The second suggests that endogenous opioids buffer normal subjects from the behavioral and physiological manifestations of the panic attack, and their deficit brings about heightened suffocation sensitivity and separation anxiety in panic patients, making...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panic; Animal model; Periaqueductal gray; Serotonin; Opioids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400011

Sulfated fucan as support for antibiotic immobilization
Provedor de dados: 56
Autores: Araújo,P.M.; Oliveira,G.B.; Córdula,C.R.; Leite,E.L.; Carvalho Jr.,L.B.; Silva,M.P.C.
Xylofucoglucuronan from Spatoglossum schröederi algae was tested as a support for antibiotic immobilization. The polysaccharide (20 mg in 6 ml) was first activated using carbodiimide, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide methiodide (20 mg in 2 ml), under stirring for 1 h at 25ºC and pH from 4.5 to 5.0. After adjusting the pH to 8.0, either gentamicin or amikacin (62.5 mg in 1.25 ml) was then immobilized on this chemically modified polysaccharide with shaking for 24 h in a cold room. Infrared spectra of the activated carbodiimide xylofucoglucuronan showed two bands to carbonyl (C = O at 1647.9 and 1700.7 cm-1) and to amide (C&lt;FONT FACE=Symbol&gt;Ý&lt;/FONT&gt;-NH2) groups (1662.8 and 1714.0 cm-1). Microbial characterization of the derivatives...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sulfated fucan; Immobilization; Antibiotics; Gentamicin; Amikacin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300002

Brain SPECT imaging in Sydenham's chorea
Provedor de dados: 56
Autores: Barsottini,O.G.P.; Ferraz,H.B.; Seviliano,M.M.; Barbieri,A.
The objective of the present study was to determine whether brain single-photon emission computed tomography (SPECT) imaging is capable of detecting perfusional abnormalities. Ten Sydenham's chorea (SC) patients, eight females and two males, 8 to 25 years of age (mean 13.4), with a clinical diagnosis of SC were submitted to brain SPECT imaging. We used HMPAO labeled with technetium-99m at a dose of 740 MBq. Six examinations revealed hyperperfusion of the basal ganglia, while the remaining four were normal. The six patients with abnormal results were females and their data were not correlated with severity of symptoms. Patients with abnormal brain SPECT had a more recent onset of symptoms (mean of 49 days) compared to those with normal SPECT (mean of 85...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sydenham's chorea; Single-photon emission computed tomography; SPECT imaging; Rheumatic fever; Brain perfusion.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400004

Sodium taste threshold in children and its relationship to blood pressure
Provedor de dados: 56
Autores: Arguelles,J.; Diaz,J.J.; Malaga,I.; Perillan,C.; Costales,M.; Vijande,M.
Popular science has emphasized the risks of high sodium intake and many studies have confirmed that salt intake is closely related to hypertension. The present mini-review summarizes experiments about salt taste sensitivity and its relationship with blood pressure (BP) and other variables of clinical and familial relevance. Children and adolescents from control parents (N = 72) or with at least one essential hypertensive (EHT) parent (N = 51) were investigated. Maternal questionnaires on eating habits and vomiting episodes were collected. Offspring, anthropometric, BP, and salt taste sensitivity values were recorded and blood samples analyzed. Most mothers declared that they added "little salt" when cooking. Salt taste sensitivity was inversely correlated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Hypertension; Cardiovascular risk factors; Salt taste sensitivity; Pregnancy; Obesity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500017

Gap junctions in isolated rat aorta: evidence for contractile responses that exhibit a differential dependence on intercellular communication
Provedor de dados: 56
Autores: Christ,G.J.; Brink,P.R.
Connexin43 (Cx43) is a major gap junction protein present in the Fischer-344 rat aorta. Previous studies have identified conditions under which selective disruption of intercellular communication with heptanol caused a significant, readily reversible and time-dependent diminution in the magnitude of &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;1-adrenergic contractions in isolated rat aorta. These observations have indentified a significant role for gap junctions in modulating vascular smooth muscle tone. The goal of these steady-state studies was to utilize isolated rat aortic rings to further evaluate the contribution of intercellular junctions to contractions elicited by cellular activation in response to several other vascular spasmogens. The effects of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gap junctions; Connexin43; Rat aorta; Vascular smooth muscle; Intercellular communication.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400008

Effect of silybin on high-fat-induced fatty liver in rats
Provedor de dados: 56
Autores: Yao,Jiayin; Zhi,Min; Minhu,Chen.
Silybin, a natural antioxidant, has been traditionally used against a variety of liver ailments. To investigate its effect and the underlying mechanisms of action on non-alcoholic fatty liver in rats, we used 60 4-6-week-old male Sprague-Dawley rats to establish fatty liver models by feeding a high-fat diet for 6 weeks. Hepatic enzyme, serum lipid levels, oxidative production, mitochondrial membrane fluidity, homeostasis model assessment-insulin resistance index (HOMA-IR), gene and protein expression of adiponectin, and resistin were evaluated by biochemical, reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. Compared with the model group, silybin treatment (26.25 mg·kg-1·day-1, started at the beginning of the protocol)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-alcoholic fatty liver disease; Mitochondrial membrane fluidity; Lipid peroxidation; Insulin resistance; Silybin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700007

Effect of exercise training and carvedilol treatment on cardiac function and structure in mice with sympathetic hyperactivity-induced heart failure
Provedor de dados: 56
Autores: Medeiro,A.; Vanzelli,A.S.; Rosa,K.T.; Irigoyen,M.C.; Brum,P.C.
The present investigation was undertaken to study the effect of β-blockers and exercise training on cardiac structure and function, respectively, as well as overall functional capacity in a genetic model of sympathetic hyperactivity-induced heart failure in mice (α2A/α2CArKO). α2A/α2CArKO and their wild-type controls were studied for 2 months, from 3 to 5 months of age. Mice were randomly assigned to control (N = 45), carvedilol-treated (N = 29) or exercise-trained (N = 33) groups. Eight weeks of carvedilol treatment (38 mg/kg per day by gavage) or exercise training (swimming sessions of 60 min, 5 days/week) were performed. Exercise capacity was estimated using a graded treadmill protocol and HR was measured by tail cuff. Fractional shortening was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Exercise training; Carvedilol treatment; Ventricular function; Cardiac remodeling; Α2A/α2CArKO mice.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900012

Adherence to drug therapy in kidney disease
Provedor de dados: 56
Autores: Magacho,E.J.C.; Ribeiro,L.C.; Chaoubah,A.; Bastos,M.G.
Non-adherence to drug therapy has not been extensively studied in patients with chronic kidney disease (CKD). The objective of the present study was to identify determinants of non-adherence to drug therapy in patients with CKD, not on dialysis. A prospective cohort study involving 149 patients was conducted over a period of 12 months. Adherence to drug therapy was evaluated by the self-report method at baseline and at 12 months. Patients who knew the type of drug(s) and the respective number of prescribed pills in use at the visit preceding the interview were considered to be adherent. Patients with cognitive decline were assessed by interviewing their caregivers. Mean patient age was 51 ± 16.7 years. Male patients predominated (60.4%). Univariate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug adherence; Chronic kidney disease; Drug therapy; Self-report; Nursing.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300014

Prevalence of diphtheria and tetanus antibodies and circulation of Corynebacterium diphtheriae in São Paulo, Brazil
Provedor de dados: 56
Autores: Divino-Goes,K.G.; Moraes-Pinto,M.I. de; Dinelli,M.I.S.; Casagrande,S.T.; Bonetti,T.C.S.; Andrade,P.R.; Weckx,L.Y.
The introduction of routine vaccination against tetanus and diphtheria in Brazil has decreased the incidence and changed the epidemiology of both diseases. We then investigated the prevalence of Corynebacterium diphtheriae carrier status and diphtheria and tetanus immunity in São Paulo, Brazil. From November 2001 to March 2003, 374 individuals were tested for the presence of C. diphtheriae in the naso-oropharynx and of serum diphtheria and tetanus antibodies. Participants were all healthy individuals without acute or chronic pathologies and they were stratified by age as follows: 0-12 months and 1-4, 5-9, 10-14, 15-24, 25-39, 40-59, and ³60 years. Antibodies were assessed using a double-antigen ELISA. C. diphtheriae species were identified by biochemical...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diphtheria; Tetanus; Corynebacterium diphtheriae; Vaccine; Immunization; Antibody.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200012

Effect of perimuscular injection of Bothrops jararacussu venom on plasma creatine kinase levels in mice: influence of dose and volume
Provedor de dados: 56
Autores: Calil-Elias,S.; Thattassery,E.; Martinez,A.M.B.; Melo,P.A.
The effect of dose and volume of a perimuscular injection of Bothrops jararacussu venom on myonecrosis of skeletal muscle was studied in mice. An increase of the venom dose (0.25 to 2.0 µg/g) at a given volume (50 µl) resulted in an increase in plasma creatine kinase (CK) levels 2 h after injection. Plasma CK activity increased from the basal level of 129.27 ± 11.83 (N = 20) to 2392.80 ± 709.43 IU/l (N = 4) for the 1.0 µg/g dose. Histological analysis of extensor digitorum longus muscle 4 h after injection showed lesion of peripheral muscle fibers, disorganization of the bundles or the complete degeneration of muscle fibers. These lesions were more extensive when higher doses were injected. Furthermore, an increase in volume (12.5 to 100 µl) by dilution of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bothrops venoms; Myotoxicity; Skeletal muscle; Plasma creatine kinase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000018

Risk factors for decreased bone density in premenopausal women
Provedor de dados: 56
Autores: Krahe,C.; Friedman,R.; Gross,J.L.
Osteoporosis is a major health problem. Little is known about the risk factors in premenopause. Sixty 40-50-year old patients with regular menses were studied cross-sectionally. None of the patients were on drugs known to interfere with bone mass. Patients answered a dietary inquiry and had their bone mineral density (BMD) measured. The Z scores were used for the comparisons. A blood sample was taken for the determination of FSH, SHBG, estradiol, testosterone, calcium and alkaline phosphatase. Calcium and creatinine were measured in 24-h urine. A Z score less than -1 was observed for the lumbar spine of 14 patients (23.3%), and for the femur of 24 patients (40%). Patients with a Z score less than -1 for the lumbar spine were older than patients with a Z...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Bone loss; Premenopause; Osteopenia; Densitometry.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900003

CPU0213, a novel endothelin type A and type B receptor antagonist, protects against myocardial ischemia/reperfusion injury in rats
Provedor de dados: 56
Autores: Wang,Z.Y.; Zhang,W.; Li,X.Z.; Han,Y.; Chen,Y.P.; Liu,Z.; Xie,L.P.; Ji,Y.; Lu,X.
The efficacy of endothelin receptor antagonists in protecting against myocardial ischemia/reperfusion (I/R) injury is controversial, and the mechanisms remain unclear. The aim of this study was to investigate the effects of CPU0123, a novel endothelin type A and type B receptor antagonist, on myocardial I/R injury and to explore the mechanisms involved. Male Sprague-Dawley rats weighing 200-250 g were randomized to three groups (6-7 per group): group 1, Sham; group 2, I/R + vehicle. Rats were subjected to in vivo myocardial I/R injury by ligation of the left anterior descending coronary artery and 0.5% sodium carboxymethyl cellulose (1 mL/kg) was injected intraperitoneally immediately prior to coronary occlusion. Group 3, I/R + CPU0213. Rats were subjected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelin receptor antagonist; Myocardial ischemia/reperfusion injury; Akt; ENOS.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100011

A randomized, double-blind, and placebo-controlled study with tranexamic acid of bleeding and fibrinolytic activity after primary coronary artery bypass grafting
Provedor de dados: 56
Autores: Santos,A.T.L.; Kalil,R.A.K.; Bauemann,C.; Pereira,J.B.; Nesralla,I.A.
Cardiopulmonary bypass is frequently associated with excessive blood loss. Platelet dysfunction is the main cause of non-surgical bleeding after open-heart surgery. We randomized 65 patients in a double-blind fashion to receive tranexamic acid or placebo in order to determine whether antifibrinolytic therapy reduces chest tube drainage. The tranexamic acid group received an intravenous loading dose of 10 mg/kg, before the skin incision, followed by a continuous infusion of 1 mg kg-1 h-1 for 5 h. The placebo group received a bolus of normal saline solution and continuous infusion of normal saline for 5 h. Postoperative bleeding and fibrinolytic activity were assessed. Hematologic data, convulsive seizures, allogeneic transfusion, occurrence of myocardial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tranexamic acid; Coronary artery bypass; Postoperative bleeding; Clinical trial.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100007

Education, tobacco smoking, alcohol consumption, and IL-2 and IL-6 gene polymorphisms in the survival of head and neck cancer
Provedor de dados: 56
Autores: López,R.V.M.; Zago,M.A.; Eluf-Neto,J.; Curado,M.P.; Daudt,A.W.; da Silva-Junior,W.A.; Zanette,D.L.; Levi,J.E.; de Carvalho,M.B.; Kowalski,L.P.; Abrahão,M.; de Góis-Filho,J.F.; Boffetta,P.; Wünsch-Filho,V.
The association of education, tobacco smoking, alcohol consumption, and interleukin-2 (IL-2 +114 and -384) and -6 (IL-6 -174) DNA polymorphisms with head and neck squamous cell carcinoma (HNSCC) was investigated in a cohort study of 445 subjects. IL-2 and IL-6 genotypes were determined by real-time PCR. Cox regression was used to estimate hazard ratios (HR) and 95% confidence intervals (95%CI) of disease-specific survival according to anatomical sites of the head and neck. Mean age was 56 years and most patients were males (87.6%). Subjects with 5 or more years of schooling had better survival in larynx cancer. Smoking had no effect on HNSCC survival, but alcohol consumption had a statistically significant effect on larynx cancer. IL-2 gene +114 G/T (HR =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smoking; Alcohol; Interleukin; Head and neck cancer; Cancer prognosis; Survival analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000005

CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
Provedor de dados: 56
Autores: Aguiar,D.P.; Coelho-Aguiar,J.M.; Abreu,J.G.
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teratocarcinoma; Adhesion; Condensation; CCN family; CCN2/CTGF; Extracellular matrix.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004

Selection of patients for myocardial perfusion scintigraphy based on fuzzy sets theory applied to clinical-epidemiological data and treadmill test results
Provedor de dados: 56
Autores: Duarte,P.S.; Mastrocolla,L.E.; Farsky,P.S.; Sampaio,C.R.E.P.S.; Tonelli,P.A.; Barros,L.C.; Ortega,N.R.; Pereira,J.C.R.
Coronary artery disease (CAD) is a worldwide leading cause of death. The standard method for evaluating critical partial occlusions is coronary arteriography, a catheterization technique which is invasive, time consuming, and costly. There are noninvasive approaches for the early detection of CAD. The basis for the noninvasive diagnosis of CAD has been laid in a sequential analysis of the risk factors, and the results of the treadmill test and myocardial perfusion scintigraphy (MPS). Many investigators have demonstrated that the diagnostic applications of MPS are appropriate for patients who have an intermediate likelihood of disease. Although this information is useful, it is only partially utilized in clinical practice due to the difficulty to properly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fuzzy model; Coronary disease; Scintigraphy; Myocardial perfusion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100002

Myocardial antioxidant and oxidative stress changes due to sex hormones
Provedor de dados: 56
Autores: Barp,J.; Araújo,A.S.R.; Fernandes,T.R.G.; Rigatto,K.V.; Llesuy,S.; Belló-Klein,A.; Singal,P.
The purpose of the present study was to examine myocardial antioxidant and oxidative stress changes in male and female rats in the presence of physiological sex hormone concentrations and after castration. Twenty-four 9-week-old Wistar rats were divided into four groups of 6 animals each: 1) sham-operated females, 2) castrated females, 3) sham-operated males, and 4) castrated males. When testosterone and estrogen levels were measured by radioimmunoassay, significant differences were observed between the castrated and control groups (both males and females), demonstrating the success of castration. Progesterone and catalase levels did not change in any group. Control male rats had higher levels of glutathione peroxidase (50%) and lower levels of superoxide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Glutathione peroxidase; Lipid peroxidation; Superoxide dismutase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900008

Cytokine profile and lymphocyte subsets in type 2 diabetes
Provedor de dados: 56
Autores: Francisco,C.O.; Catai,A.M.; Moura-Tonello,S.C.G.; Arruda,L.C.M.; Lopes,S.L.B.; Benze,B.G.; Del Vale,A.M.; Malmegrim,K.C.R.; Leal,A.M.O.
Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Cytokines; Lymphocytes; Inflammation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400707

Involvement of glutamate and reactive oxygen species in methylmercury neurotoxicity
Provedor de dados: 56
Autores: Aschner,M.; Syversen,T.; Souza,D.O.; Rocha,J.B.T.; Farina,M.
This review addresses the mechanisms of methylmercury (MeHg)-induced neurotoxicity, specifically examining the role of oxidative stress in mediating neuronal damage. A number of critical findings point to a central role for astrocytes in mediating MeHg-induced neurotoxicity as evidenced by the following observations: a) MeHg preferentially accumulates in astrocytes; b) MeHg specifically inhibits glutamate uptake in astrocytes; c) neuronal dysfunction is secondary to disturbances in astrocytes. The generation of reactive oxygen species (ROS) by MeHg has been observed in various experimental paradigms. For example, MeHg enhances ROS formation both in vivo (rodent cerebellum) and in vitro (isolated rat brain synaptosomes), as well as in neuronal and mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylmercury neurotoxicity; Oxidative stress; Glutamate and selenocompounds; Reactive oxygen species; Astrocytes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300001

Combination therapy of orally administered glycyrrhizin and UVB improved active-stage generalized vitiligo
Provedor de dados: 56
Autores: Mou,K.H.; Han,D.; Liu,W.L.; Li,P.
Glycyrrhizin has been used clinically for several years due to its beneficial effect on immunoglobulin E (IgE)-induced allergic diseases, alopecia areata and psoriasis. In this study, glycyrrhizin, ultraviolet B light (UVB) or a combination of both were used to treat active-stage generalized vitiligo. One hundred and forty-four patients between the ages of 3 and 48 years were divided into three groups: group A received oral compound glycyrrhizin (OCG); group B received UVB applications twice weekly, and group C received OCG+UVB. Follow-ups were performed at 2, 4, and 6 months after the treatment was initiated. The Vitiligo Area Scoring Index (VASI) and the Vitiligo Disease Activity (VIDA) instrument were used to assess the affected body surface, at each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycyrrhizin; UVB; Generalized vitiligo; Disease stage; Active stage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800605

Deficiency of sex hormones does not affect 17-ß-estradiol-induced coronary vasodilation in the isolated rat heart
Provedor de dados: 56
Autores: Santos,R.L.; Lima,J.T.; Rouver,W.N.; Moysés,M.R.
The relaxation of coronary arteries by estrogens in the coronary vascular beds of naive and hypertensive rats has been well described. However, little is known about this action in gonadectomized rats. We investigated the effect of 17-ß-estradiol (E2) in coronary arteries from gonadectomized rats, as well as the contributions of endothelium-derived factors and potassium channels. Eight-week-old female and male Wistar rats weighing 220-300 g were divided into sham-operated and gonadectomized groups (n=9−12 animals per group). The baseline coronary perfusion pressure (CPP) was determined, and the vasoactive effects of 10 μM E2 were assessed by bolus administration before and after endothelium denudation or by perfusion with NG-nitro-L-arginine methyl ester...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gonadectomy; Estrogen; Vasodilation; Coronary arteries.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500603

Evidence of muscarinic acetylcholine receptors in the retinal centrifugal system of the chick
Provedor de dados: 56
Autores: Calaza,K.C.; Gardino,P.F.
In this study we characterize the presence of muscarinic acetylcholine receptors (mAChR) in the isthmo-optic nucleus (ION) of chicks by immunohistochemistry with the M35 antibody. Some M35-immunoreactive fibers were observed emerging from the retinal optic nerve insertion, suggesting that they could be centrifugal fibers. Indeed, intraocular injections of cholera toxin B (CTb), a retrograde tracer, and double-labeling with M35 and CTb in the ION confirmed this hypothesis. The presence of M35-immunoreactive cells and the possible mAChR expression in ION and ectopic neuron cells in the chick brain strongly suggest the existence of such a cholinergic system in this nucleus and that acetylcholine release from amacrine cells may mediate interactions between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscarinic receptors; Retina; Isthmo-optic nucleus; Centrifugal fibers; Acetylcholine; Chick.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900014

Antidepressant-like effect of lead in adult mice
Provedor de dados: 56
Autores: Mantovani,M.; Matteussi,A.S.; Rodrigues,A.L.S.
It has been reported that lead can cause behavioral impairment by inhibiting the N-methyl-D-aspartate (NMDA) receptor complex. MK-801, a noncompetitive NMDA receptor antagonist, exhibits an antidepressant-like action in the forced swimming test. The purpose of the present study was to determine whether subacute lead exposure in adult male Swiss mice weighing 30-35 g causes an antidepressant-like action in a forced swimming test. Mice were injected intraperitoneally (ip) with 10 mg/kg lead acetate or saline daily for 7 consecutive days. Twenty-four hours after the last treatment, the saline and lead-treated mice received an injection of MK-801 (0.01 mg/kg, ip) or saline and were tested in forced swimming and in open-field tests. Immobility time was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lead acetate; Forced swimming test; Depression; Open-field.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200017

Methodological approaches to planar and volumetric scintigraphic imaging of small volume targets with high spatial resolution and sensitivity
Provedor de dados: 56
Autores: Mejia,J.; Galvis-Alonso,O.Y.; Braga,J.; Corrêa,R.; Leite,J.P.; Simões,M.V.
Single-photon emission computed tomography (SPECT) is a non-invasive imaging technique, which provides information reporting the functional states of tissues. SPECT imaging has been used as a diagnostic tool in several human disorders and can be used in animal models of diseases for physiopathological, genomic and drug discovery studies. However, most of the experimental models used in research involve rodents, which are at least one order of magnitude smaller in linear dimensions than man. Consequently, images of targets obtained with conventional gamma-cameras and collimators have poor spatial resolution and statistical quality. We review the methodological approaches developed in recent years in order to obtain images of small targets with good spatial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Small animal SPECT; Multipinhole collimators; Coded masks.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800001

Studies on the intrinsic nervous system of the wild rodent Calomys callosus digestive tract. II: The submucous plexus
Provedor de dados: 56
Autores: Souza,N.B.; Liberti,E.A.; De-Souza,R.R.
The submucous plexus of the normal small and large intestine of Calomys callosus was studied by NADH and AChE histochemical techniques and by transmission and scanning electron microscopy. The plexus contains (mean ± SD) 7,488 ± 293 neurons/cm2 in the duodenum, 5,611 ± 836 in the jejunum, 2,741 ± 360 in the ileum, 3,067 ± 179 in the cecum, and 3,817 ± 256 in the proximal colon. No ganglia or nerve cell bodies were seen in the esophagus, stomach, distal colon or rectum. The neurons are pear-shaped with a round or oval nucleus and the neuronal cell profile areas were larger in the large intestine than in the small intestine. Most of the neurons display intense AChE activity in the cytoplasm. AChE-positive nerve fibers are present in a primary meshwork of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Submucous plexus; Wild mouse; Morphometry; Histochemistry; Ultrastructure; Calomys callosus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500007

Interleukin-10-dependent down-regulation of interferon-gamma response to Leishmania by Mycobacterium leprae antigens during the clinical course of a coinfection
Provedor de dados: 56
Autores: Azeredo-Coutinho,R.B.G.; Matos,D.C.S.; Nery,J.A.C.; Valete-Rosalino,C.M.; Mendonça,S.C.F.
We have described a case of a patient with an intriguing association of mucocutaneous leishmaniasis with lepromatous leprosy, two opposite polar forms of these spectral diseases. In the present follow-up study, we investigated the effect of the addition of Mycobacterium leprae antigens on interferon-gamma (IFN-γ) production in Leishmania antigen-stimulated cultures of peripheral blood mononuclear cells (PBMC) from this patient. For this purpose, PBMC cultures were stimulated with crude L. braziliensis and/or M. leprae whole-cell antigen extracts or with concanavalin A. In some experiments, neutralizing anti-human interleukin (IL)-10 antibodies were added to the cultures. IFN-γ and IL-10 levels in culture supernatants were measured by ELISA. During active...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Interferon-gamma; Interleukin-10; Mucocutaneous leishmaniasis; Lepromatous leprosy; Leishmania braziliensis; Mycobacterium leprae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700010

Treatment with ascorbic acid and α-tocopherol modulates oxidative-stress markers in the spinal cord of rats with neuropathic pain
Provedor de dados: 56
Autores: Riffel,A.P.K.; Santos,M.C.Q.; de Souza,J.A.; Scheid,T.; Horst,A.; Kolberg,C.; Belló-Klein,A.; Partata,W.A.
Vitamin E (vit. E) and vitamin C (vit. C) are antioxidants that inhibit nociception. The effect of these vitamins on oxidative-stress markers in the spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve is unknown. This study investigated the effect of intraperitoneal administration of vit. E (15 mg·kg-1·day-1) and vit. C (30 mg·kg-1·day-1), given alone or in combination, on spinal cord oxidative-stress markers in CCI rats. Adult male Wistar rats weighing 200–250 g were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total thiol; Superoxide anion generation; Nitric oxide; Lipid hydroperoxides; Hydrogen peroxide; Total antioxidant capacity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400612

Excitotoxic median raphe lesions aggravate working memory storage performance deficits caused by scopolamine infusion into the dentate gyrus of the hippocampus in the inhibitory avoidance task in rats
Provedor de dados: 56
Autores: Babar,E.; Melik,E.; Özgünen,T.
The interactions between the median raphe nucleus (MRN) serotonergic system and the septohippocampal muscarinic cholinergic system in the modulation of immediate working memory storage performance were investigated. Rats with sham or ibotenic acid lesions of the MRN were bilaterally implanted with cannulae in the dentate gyrus of the hippocampus and tested in a light/dark step-through inhibitory avoidance task in which response latency to enter the dark compartment immediately after the shock served as a measure of immediate working memory storage. MRN lesion per se did not alter response latency. Post-training intrahippocampal scopolamine infusion (2 and 4 µg/side) produced a more marked reduction in response latencies in the lesioned animals compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Working memory; Median raphe nucleus; Serotonin; Scopolamine; Acetylcholine; Inhibitory avoidance.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400012

New multilocus sequence typing of MRSA in São Paulo, Brazil
Provedor de dados: 56
Autores: Carmo,M.S.; Inoue,F.; Andrade,S.S.; Paschoal,L.; Silva,F.M.; Oliveira,V.G.S.; Pignatari,A.C.C.
An increased incidence of nosocomial and community-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has been observed worldwide. The molecular characterization of MRSA has played an important role in demonstrating the existence of internationally disseminated clones. The use of molecular biology methods in the surveillance programs has enabled the tracking of MRSA spread within and among hospitals. These data are useful to alert nosocomial infection control programs about the potential introduction of these epidemic clones in their areas. Four MRSA blood culture isolates from patients hospitalized at two hospitals in the city of São Paulo, Brazil, were analyzed; one of them was community acquired. The isolates were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular sequence typing; Resistance; Molecular typing.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000006

Acute exposure to lead increases myocardial contractility independent of hypertension development
Provedor de dados: 56
Autores: Fioresi,M.; Furieri,L.B.; Simões,M.R.; Ribeiro Junior,R.F.; Meira,E.F.; Fernandes,A.A.; Stefanon,I.; Vassallo,D.V.
We studied the effects of the acute administration of small doses of lead over time on hemodynamic parameters in anesthetized rats to determine if myocardial contractility changes are dependent or not on the development of hypertension. Male Wistar rats received 320 µg/kg lead acetateiv once, and their hemodynamic parameters were measured for 2 h. Cardiac contractility was evaluated in vitro using left ventricular papillary muscles as were Na+,K+-ATPase and myosin Ca2+-ATPase activities. Lead increased left- (control: 112 ± 3.7 vs lead: 129 ± 3.2 mmHg) and right-ventricular systolic pressures (control: 28 ± 1.2vs lead: 34 ± 1.2 mmHg) significantly without modifying heart rate. Papillary muscles were exposed to 8 µM lead acetate and evaluated 60 min later....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lead; Myocardial contractility; Ventricular pressure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200178

Differential effects of lead and zinc on inhibitory avoidance learning in mice
Provedor de dados: 56
Autores: de Oliveira,F.S.; Viana,M.R.; Antoniolli,A.R.; Marchioro,M.
We studied the effects of chronic intoxication with the heavy metals lead (Pb2+) and zinc (Zn2+) on memory formation in mice. Animals were intoxicated through drinking water during the pre- and postnatal periods and then tested in the step-through inhibitory avoidance memory task. Chronic postnatal intoxication with Pb2+ did not change the step-through latency values recorded during the 4 weeks of the test (ANOVA, P&gt;0.05). In contrast, mice intoxicated during the prenatal period showed significantly reduced latency values when compared to the control group (day 1: q = 4.62, P&lt;0.05; day 7: q = 4.42, P&lt;0.05; day 14: q = 5.65, P&lt;0.05; day 21: q = 3.96, P&lt;0.05, and day 28: q = 6.09, P&lt;0.05). Although chronic postnatal intoxication with Zn2+...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lead; Zinc; Inhibitory avoidance; Memory.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100014

The photodynamic and non-photodynamic actions of porphyrins
Provedor de dados: 56
Autores: Afonso,S.G.; Enríquez de Salamanca,R.; Batlle,A.M. del C.
Porphyrias are a family of inherited diseases, each associated with a partial defect in one of the enzymes of the heme biosynthetic pathway. In six of the eight porphyrias described, the main clinical manifestation is skin photosensitivity brought about by the action of light on porphyrins, which are deposited in the upper epidermal layer of the skin. Porphyrins absorb light energy intensively in the UV region, and to a lesser extent in the long visible bands, resulting in transitions to excited electronic states. The excited porphyrin may react directly with biological structures (type I reactions) or with molecular oxygen, generating excited singlet oxygen (type II reactions). Besides this well-known photodynamic action of porphyrins, a novel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic; Porphyrias; Porphyrins; Protein alterations.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300002

Cost-effective analysis of different algorithms for the diagnosis of hepatitis C virus infection
Provedor de dados: 56
Autores: Barreto,A.M.E.C.; Takei,K.; E.C.,Sabino; Bellesa,M.A.O.; Salles,N.A.; Barreto,C.C.; Nishiya,A.S.; Chamone,D.F.
We compared the cost-benefit of two algorithms, recently proposed by the Centers for Disease Control and Prevention, USA, with the conventional one, the most appropriate for the diagnosis of hepatitis C virus (HCV) infection in the Brazilian population. Serum samples were obtained from 517 ELISA-positive or -inconclusive blood donors who had returned to Fundação Pró-Sangue/Hemocentro de São Paulo to confirm previous results. Algorithm A was based on signal-to-cut-off (s/co) ratio of ELISA anti-HCV samples that show s/co ratio ³95% concordance with immunoblot (IB) positivity. For algorithm B, reflex nucleic acid amplification testing by PCR was required for ELISA-positive or -inconclusive samples and IB for PCR-negative samples. For algorithm C, all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Immunoblot; ELISA; Polymerase chain reaction; Algorithm; Diagnosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200009

Use of erythromycin for the treatment of severe chronic constipation in children
Provedor de dados: 56
Autores: Bellomo-Brandão,M.A.; Collares,E.F.; da-Costa-Pinto,E.A.L.
The efficacy of erythromycin was assessed in the treatment of 14 children aged 4 to 13 years with refractory chronic constipation, and presenting megarectum and fecal impaction. A double-blind, placebo- controlled, crossover study was conducted at the Pediatric Gastroenterology Outpatient Clinic of the University Hospital. The patients were randomized to receive placebo for 4 weeks followed by erythromycin estolate, 20 mg kg-1 day-1, divided into four oral doses for another 4 weeks, or vice versa. Patient outcome was assessed according to a clinical score from 12 (most severe clinical condition) to 0 (complete recovery). At enrollment in the study and on the occasion of follow-up medical visits at two-week intervals, patient score and laxative requirements...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gastrointestinal motility; Constipation; Children; Prokinetics; Erythromycin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000016

The maternal JAK/STAT pathway of Drosophila regulates embryonic dorsal-ventral patterning
Provedor de dados: 56
Autores: Lopes,E.S.S.; Araujo,H.M.
Activation of NFkappaB plays a pivotal role in many cellular processes such as inflammation, proliferation and apoptosis. In Drosophila, nuclear translocation of the NFkappaB-related transcription factor Dorsal is spatially regulated in order to subdivide the embryo into three primary dorsal-ventral (DV) domains: the ventral presumptive mesoderm, the lateral neuroectoderm and the dorsal ectoderm. Ventral activation of the Toll receptor induces degradation of the IkappaB-related inhibitor Cactus, liberating Dorsal for nuclear translocation. In addition, other pathways have been suggested to regulate Dorsal. Signaling through the maternal BMP member Decapentaplegic (Dpp) inhibits Dorsal translocation along a pathway parallel to and independent of Toll. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: JAK kinase; Drosophila; Embryonic patterning; Hopscotch; NFkappaB.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200006

The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway
Provedor de dados: 56
Autores: Chen,Jian Xia; Yi,Xi Juan; Gu,Pei Ling; Gao,Shan Xia.
The aim of this study was to investigate the role of kinase-insert domain-containing receptor (KDR) in intrauterine adhesions (IUA) and its mechanism. The Case group consisted of 92 patients diagnosed with IUA, and the Control group included 86 patients with uterine septum who had normal endometrium verified with an uteroscope. In addition, 50 rats were randomly assigned into Control, Sham, Model, NC-siRNA, and KDR-siRNA groups. Rats in the Model, NC-siRNA, and KDR-siRNA groups were induced by uterine curettage and lipopolysaccharide (LPS) treatment to establish the IUA model. Then, immunohistochemistry was applied for detection of VEGF and KDR expression, HE staining was used for observation of the endometrial morphology and gland counting, Masson...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: KDR; Intrauterine adhesions; TGF-β1/Smads; MMP9; VEGF.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000607

Ischemic preconditioning reduces peripheral oxidative damage associated with brain ischemia in rats
Provedor de dados: 56
Autores: Frassetto,S.S.; Schetinger,M.R.C.; Webber,A.; Sarkis,J.J.F.; Netto,C.A.
Brain ischemia followed by reperfusion causes neuronal death related to oxidative damage. Furthermore, it has been reported that subjects suffering from ischemic cerebrovascular disorders exhibit changes in circulating platelet aggregation, a characteristic that might be important for their clinical outcome. In the present investigation we studied tert-butyl hydroperoxide-initiated plasma chemiluminescence and thiol content as measures of peripheral oxidative damage in naive and preconditioned rats submitted to forebrain ischemia produced by the 4-vessel occlusion method. Rats were submitted to 2 or 10 min of global transient forebrain ischemia followed by 60 min or 1, 2, 5, 10 or 30 days of reperfusion. Preconditioned rats were submitted to a 10-min...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain ischemia; Ischemic preconditioning; Oxidative damage; Hydroperoxide-initiated chemiluminescence; Plasma thiols.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000017

Pain and distress outcomes in infants and children: a systematic review
Provedor de dados: 56
Autores: Oliveira,N.C.A.C.; Gaspardo,C.M.; Linhares,M.B.M.
The aim of the present study was to systematically review the recent literature about pain and distress outcomes in children and critically analyze the methodological quality of the reports. The systematic review was based on the PRISMA statement and performed by selecting articles that are indexed in scientific databases. The methodological quality of reports was examined using STROBE statement, for observational studies, and CONSORT statement, for randomized controlled trials. The PedIMMPACT consensus was used to evaluate the psychometric quality of pain instruments. We analyzed 23 empirical studies, including 14 randomized controlled trials, seven cross-sectional studies, and two studies with cohort designs. Fourteen studies included preschool- and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Development; Distress; Pain; Review.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700302

Fecal androgen levels in common marmoset (Callithrix jacchus) males living in captive family groups
Provedor de dados: 56
Autores: Castro,D.C.; Sousa,M.B.C.
In captive common marmoset groups, the reproductive inhibition observed in subordinate female seems to be a result of olfactory, visual and behavioral cues from the dominant female. However, few studies have examined the relationship among adult males living in the same social group. These studies have shown that reproductive failure among peer males seems to be based on hormonal and behavioral mechanisms. New insights on sexual strategies in primates have been shown using fecal steroids, but so far no information is available for common marmoset males. In the present study, we evaluated the influence of light-dark cycle, age and reproductive condition on the profile of fecal androgens in males living in the same family group. Feces were collected from six...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Common marmosets; Fecal androgens; Male sexual strategies; Circadian variation; Reproductive suppression.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100011

Female novelty and the courtship behavior of male guinea pigs (Cavia porcellus)
Provedor de dados: 56
Autores: Cohn,D.W.H.; Tokumaru,R.S.; Ades,C.
In several rodent species, an increase or recovery of sexual behavior can be observed when sexually satiated males are placed in contact with a novel mate. In order to assess the influence of female novelty on the courtship behavior of guinea pigs (Cavia porcellus), four adult males were observed during four daily 15-min sessions while interacting with the same pregnant female (same-female sessions). A new female was presented during the fifth session (switched-female session). The duration of behavioral categories was obtained from videotape records using an observational software. From the first to the second session, all males decreased the time allocated to investigating (sniffing and licking), following, and mounting the female, and that response did...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Courtship behavior; Female novelty; Guinea pigs; Cavia porcellus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600010

Pathogenesis of gallbladder adenomyomatosis and its relationship with early-stage gallbladder carcinoma: an overview
Provedor de dados: 56
Autores: Pang,Liwei; Zhang,Yan; Wang,Yuwen; Kong,Jing.
The exact pathogenesis of gallbladder adenomyomatosis is still lacking and some controversies over its diagnosis and treatment exist. Originally recognized as a precancerous lesion, adenomyomatosis is currently recognized by recent studies as a benign alteration of the gallbladder that is often associated with cholecystitis and cholecystolithiasis. Gallbladder carcinoma is an extremely malignant disease with a 5-year survival rate of less than 5%. Therefore, it is important to diagnose, differentiate, and confirm the relationship between adenomyomatosis and early-stage gallbladder carcinoma. However, the early clinical symptoms of adenomyomatosis are extremely similar to those of gallbladder stones and cholecystitis, increasing the difficulty to identify...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallbladder adenomyomatosis; Carcinoma; Rokitansky-Aschoff sinuses; Review.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600201

Sex hormone modulation of serotonin-induced coronary vasodilation in isolated heart
Provedor de dados: 56
Autores: Moysés,M.R.; Barker,L.A.; Cabral,A.M.
The present study was designed to evaluate the differences in the coronary vasodilator actions of serotonin (5-HT) in isolated heart obtained from naive or castrated male and female rats that were treated with either estrogen or testosterone. Hearts from 12 groups of rats were used: male and female naive animals, castrated, castrated and treated with 17ß-estradiol (0.5 µg kg-1 day-1) for 7 or 30 days, and castrated and treated with testosterone (0.5 mg kg-1 day-1) for 7 or 30 days. After treatment, the vascular reactivity of the coronary bed was evaluated. Baseline coronary perfusion pressure (CPP) was determined and dose-response curves to 5-HT were generated. Baseline CPP differed between male (70 ± 6 mmHg, N = 10) and female (115 ± 6 mmHg, N = 12) naive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary circulation; Endothelial function; Estradiol; Serotonin; Smooth muscle.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700014

Typing class I HLA-A gene using a nested PCR-RFLP procedure
Provedor de dados: 56
Autores: Castelli,E.C.; Gil,D.S.; Veiga,L.C.S.; de Camargo,J.L.V.
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human leukocyte antigen-A; HLA-A; Typing; RFLP; Polymorphism; Major histocompatibility complex.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600004

Erythrocyte glucose-6-phosphate dehydrogenase from Brazilian opossum Didelphis marsupialis
Provedor de dados: 56
Autores: Barretto,O.C. de O.; Oshiro,M.; Oliveira,R.A.G.; Fedullo,J.D.L.; Nonoyama,K.
In a comparative study of erythrocyte metabolism of vertebrates, the specific activity of glucose-6-phosphate dehydrogenase (G6PD) of the Brazilian opossum Didelphis marsupialis in a hemolysate was shown to be high, 207 ± 38 IU g-1 Hb-1 min-1 at 37ºC, compared to the human erythrocyte activity of 12 ± 2 IU g-1 Hb-1 min-1 at 37ºC. The apparent high specific activity of the mixture led us to investigate the physicochemical properties of the opossum enzyme. We report that reduced glutathione (GSH) in the erythrocytes was only 50% higher than in human erythrocytes, a value lower than expected from the high G6PD activity since GSH is maintained in a reduced state by G6PD activity. The molecular mass, determined by G-200 Sephadex column chromatography at pH 8.0,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Erythrocyte glucose-6-phosphate dehydrogenase; Didelphis marsupialis; Erythrocyte glutathione.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500007

Differential expression of microRNA-411 and 376c is associated with hypertension in pregnancy
Provedor de dados: 56
Autores: Yang,Hui-li; Zhang,Hong-zhi; Meng,Fan-rong; Han,Shu-yi; Zhang,Miao.
Preeclampsia is a major reason of morbidity and mortality in pregnant women and perinatal fetus. Hence, it is of prime importance that diagnostic markers are defined to predict chances of preeclampsia in pregnant women. It has been previously shown that microRNA (miRNA)-376c expression is decreased in the placenta of preeclampsia patients at term. Even though this decrease was not mimicked in the placenta at the pre-term stage, miR-376c expression was decreased in the plasma of these patients as early as the second trimester. Plasma and placenta specimens were obtained from pregnant women having unifetal gestation undergoing perinatal care between January 2014 and December 2016 (n=49). Early trimester placentas were collected from patients undergoing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Pregnancy; Hypertension; MiR-411; MiR-376c.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400607

Surgical treatment of Denis type B thoracolumbar burst fracture with neurological deficiency by paraspinal approach
Provedor de dados: 56
Autores: Wu,H.; Zhao,D.-X.; Jiang,R.; Zhou,X.-Y.
We aimed to describe the surgical technique and clinical outcomes of paraspinal-approach reduction and fixation (PARF) in a group of patients with Denis type B thoracolumbar burst fracture (TLBF) with neurological deficiencies. A total of 62 patients with Denis B TLBF with neurological deficiencies were included in this study between January 2009 and December 2011. Clinical evaluations including the Frankel scale, pain visual analog scale (VAS) and radiological assessment (CT scans for fragment reduction and X-ray for the Cobb angle, adjacent superior and inferior intervertebral disc height, and vertebral canal diameter) were performed preoperatively and at 3 days, 6 months, and 1 and 2 years postoperatively. All patients underwent successful PARF, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thoracolumbar burst fracture; Paraspinal approach; Neurological deficiency; Surgery treatment.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100604

ChIP-seq analysis of histone H3K9 trimethylation in peripheral blood mononuclear cells of membranous nephropathy patients
Provedor de dados: 56
Autores: Sui,W.G.; He,H.Y.; Yan,Q.; Chen,J.J.; Zhang,R.H.; Dai,Y.
Membranous nephropathy (MN), characterized by the presence of diffuse thickening of the glomerular basement membrane and subepithelial in situimmune complex disposition, is the most common cause of idiopathic nephrotic syndrome in adults, with an incidence of 5-10 per million per year. A number of studies have confirmed the relevance of several experimental insights to the pathogenesis of human MN, but the specific biomarkers of MN have not been fully elucidated. As a result, our knowledge of the alterations in histone methylation in MN is unclear. We used chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze the variations in a methylated histone (H3K9me3) in peripheral blood mononuclear cells from 10 MN patients and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ChIP-seq; Epigenetics; H3K9me3; Membranous nephropathy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100042

Serial blood pressure measurements
Provedor de dados: 56
Autores: Koehler,N.R.; Figueiredo,C.E.P.; Ribeiro,A.C.M.
The objective of the present investigation was to study the effects of a 60-s interval of venous congestion between two noninvasive measurements of arterial blood pressure (ABP) on the fluctuation of ABP, assessed by the standard deviation of the differences between two readings. ABP was measured in 345 successive patients, at rest, four times each. For 269 participants, one pair of readings was obtained with a 60-s interval and the other pair without an interval. For 76 patients, the first pair was read at the same interval, and the second pair had venous congestion interposed and there was no waiting interval. There was no increased ABP oscillation, either when there was no interval between ABP readings, or when venous congestion was interposed compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood pressure; Arterial blood pressure oscillations; Venous congestion; Arterial blood pressure measurement technique; Arterial blood pressure fluctuations in the short term.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500007

Cat ownership is associated with increased asthma prevalence and dog ownership with decreased spirometry values
Provedor de dados: 56
Autores: Simoneti,C.S.; Ferraz,E.; Menezes,M.B.; Icuma,T.R.; Vianna,E.O.
The association between pet ownership and the development of allergic and respiratory diseases has been the aim of several studies, however, the effects of exposure in adults remain uncertain. The aims of the present study were to investigate the prevalence of asthma and lung function status among dog and cat owners. This cross-sectional study was performed at two universities with students and workers who were allocated into 3 groups according to pet ownership in the previous year: cat owners, dog owners, and no pets (control group). Subjects underwent spirometry, bronchial challenge test with mannitol, skin prick tests, and questionnaires about animal exposures and respiratory symptoms. Control group comprised 125 subjects; cat owner group, 51 subjects;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Respiratory tract diseases; Animals; Bronchial hyperreactivity; Lung function; Pets.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200601

Thermodynamic evaluation and modeling of proton and water exchange associated with benzamidine and berenil binding to ß-trypsin
Provedor de dados: 56
Autores: Pereira,M.T.; Silva-Alves,J.M.; Martins-José,A.; Lopes,J.C.D.; Santoro,M.M.
Serine-proteases are involved in vital processes in virtually all species. They are important targets for researchers studying the relationships between protein structure and activity, for the rational design of new pharmaceuticals. Trypsin was used as a model to assess a possible differential contribution of hydration water to the binding of two synthetic inhibitors. Thermodynamic parameters for the association of bovine ß-trypsin (homogeneous material, observed 23,294.4 ± 0.2 Da, theoretical 23,292.5 Da) with the inhibitors benzamidine and berenil at pH 8.0, 25ºC and with 25 mM CaCl2, were determined using isothermal titration calorimetry and the osmotic stress method. The association constant for berenil was about 12 times higher compared to the one for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benzamidine; Berenil; Calorimetry; Protein modeling; Trypsin; Osmotic stress.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100005

Western blot detection of infectious bursal disease virus infection
Provedor de dados: 56
Autores: Pereira,S.R.F.G.; Travassos,C.E.P.F.; Huguenim,A.; Guimarães,A.C.C.; Silva,A.G.; Guimarães,M.A.A.M.
In order to evaluate the use of a Western blot methodology for the diagnosis of infectious bursal disease virus (IBDV) infection, chickens were experimentally infected with IBDV strains and tested for the presence of viral antigens and antibodies by a blocking Western blot test (bWB). The viral proteins obtained from the bursa of Fabricius (BF) were transferred to a nitrocellulose membrane after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the chicken sera obtained by heart puncture were used for the detection of these proteins. In order to eliminate nonspecific reactions, we used a rabbit anti-chicken serum (blocking tool). By the use of the bWB test, two distinct viral proteins of 43-kDa (VP2) and 32-kDa (VP3) were detected....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Western blot; IBDV infection; Chicken.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500011

Measurement of the ultrasonic properties of human coronary arteries in vitro with a 50-MHz acoustic microscope
Provedor de dados: 56
Autores: Machado,J.C.; Foster,F.S.; Gotlieb,A.I.
Ultrasonic attenuation coefficient, wave propagation speed and integrated backscatter coefficient (IBC) of human coronary arteries were measured in vitro over the -6 dB frequency bandwidth (36 to 67 MHz) of a focused ultrasound transducer (50 MHz, focal distance 5.7 mm, f/number 1.7). Corrections were made for diffraction effects. Normal and diseased coronary artery sub-samples (N = 38) were obtained from 10 individuals at autopsy. The measured mean ± SD of the wave speed (average over the entire vessel wall thickness) was 1581.04 ± 53.88 m/s. At 50 MHz, the average attenuation coefficient was 4.99 ± 1.33 dB/mm with a frequency dependence term of 1.55 ± 0.18 determined over the 36- to 67-MHz frequency range. The IBC values were: 17.42 ± 13.02 (sr.m)-1 for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acoustic microscopy; Coronary artery wall; Ultrasound tissue characterization; Integrated backscatter coefficient.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800006

HIV-1 binding and neutralizing antibodies of injecting drug users
Provedor de dados: 56
Autores: Ouverney,E.P.; Teixeira,S.L.M.; Silva-de-Jesus,C.; Hacker,M.A.; Morgado,M.G.; Bastos,F.I.; Bongertz,V.
Previous studies have demonstrated a stronger seroreactivity against some synthetic peptides responsible for inducing neutralizing antibodies in injecting drug users (IDU) compared to that of individuals sexually infected with HIV-1 (S), but the effectiveness in terms of the neutralizing ability of these antibodies has not been evaluated. Our objective was to study the humoral immune response of IDU by determining the specificity of their antibodies and the presence of neutralizing antibodies. The neutralization capacity against the HIV-1 isolate MN (genotype B), the primary HIV-1 isolate 95BRRJ021 (genotype F), and the seroreactivity with peptides known to induce neutralizing antibodies, from the V2 and V3 loops of different HIV-1 subtypes, were analyzed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Injecting drug users; Antibodies; Neutralization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900004

Normal skin of HIV-infected individuals contains increased numbers of dermal CD8 T cells and normal numbers of Langerhans cells
Provedor de dados: 56
Autores: Galhardo,M.C.G.; Alvarenga,F.F.; Schueler,G.; Perez,M.; Morgado,M.G.; Ferreira,H.; Azevedo,L.M.S.; Sampaio,E.P.; Sarno,E.N.
Dysregulation of the skin immune system (SIS) could explain the high prevalence of skin disorders in HIV+ individuals. The present study was carried out to determine whether alterations in the cell population of SIS and epidermal immunoactivation occur in the normal skin of HIV+ individuals. Forty-five biopsies were taken from the normal upper arm skin of 45 HIV+ patients and of 15 healthy controls. HIV+ individuals were divided into three categories according to their CD4 cell blood count (&lt;200, 200-499 and ³500/µl). Hematoxylin-eosin was used to stain tissue sections for morphological analysis and immunohistochemistry was used for the evaluation of the frequency of macrophages, Langerhans cells, and CD lymphocyte subsets. In addition, semiquantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Skin immune system; CD8 T cells; CD4-CD8 ratio; Langerhans cells.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500017

Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels
Provedor de dados: 56
Autores: Almeida,S.; Hutz,M.H.
Estrogen has multiple effects on lipid and lipoprotein metabolism. We investigated the association between the four common single nucleotide polymorphisms in the estrogen receptor 1 (ESR1) gene locus, -1989T&gt;G, +261G&gt;C, IVS1-397T&gt;C and IVS1-351A&gt;G, and lipid and lipoprotein levels in southern Brazilians. The sample consisted in 150 men and 187 premenopausal women. The women were considered premenopausal if they had regular menstrual bleeding within the previous 3 months and were 18-50 years of age. Exclusion criteria were pregnancy, secondary hyperlipidemia due to renal, hepatic or thyroid disease, and diabetes. Smoking status was self-reported; subjects were classified as never smoked and current smokers. DNA was amplified by PCR and was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estrogen; Estrogen receptor 1 variants; Lipid levels; Smoking.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000007

TIMP-1 mediates the inhibitory effect of interleukin-6 on the proliferation of a hepatocarcinoma cell line in a STAT3-dependent manner
Provedor de dados: 56
Autores: Guo,S.-Y.; Shen,X.; Yang,J.; Yuan,J.; Yang,R.-L.; Mao,K.; Zhao,D.-H.; Li,C.-J.
The tissue inhibitor of metalloproteinases (TIMP)-1 is a multifunctional protein which is not only an inhibitor of matrix metalloproteinases (MMPs) but also to have a possible "cytokine-like" action. Here, we first compared mRNA expression of TIMP-1 and MMP-9 in BEL-7402 (a hepatocellular carcinoma cell line), L-02 (a normal liver cell line) and QSG-7701 (a cell line derived from peripheral tissue of liver carcinoma) using real-time quantitative RT-PCR. By evaluating the variation of the MMP-9/TIMP-1 ratio as an index of reciprocal changes of the expression of the two genes, we observed that the MMP-9/TIMP-1 ratio was about 13- and 5-fold higher in BEL-7402 than in L-02 and QSG-7701, respectively. Significantly, overexpression of TIMP-1 decreased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue inhibitor of metalloproteinases-1; Hepatocellular carcinoma; Interleukin-6; STAT3; P21.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500004

Tonic and reflex cardiovascular autonomic control in trained-female rats
Provedor de dados: 56
Autores: Sanches,I.C.; Sartori,M.; Jorge,L.; Irigoyen,M.C.; De Angelis,K.
The effects of exercise training on cardiovascular and autonomic functions were investigated in female rats. After an aerobic exercise training period (treadmill: 5 days/week for 8 weeks), conscious female Wistar (2 to 3 months) sedentary (S, N = 7) or trained rats (T, N = 7) were cannulated for direct arterial pressure (AP) recording in the non-ovulatory phases. Vagal (VT) and sympathetic tonus (ST) were evaluated by vagal (atropine) and sympathetic (propranolol) blockade. Baroreflex sensitivity was evaluated by the heart rate responses induced by AP changes. Cardiopulmonary reflex was measured by the bradycardic and hypotensive responses to serotonin. Resting bradycardia was observed in T (332 ± 7 bpm) compared with S animals (357 ± 10 bpm), whereas AP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex; Cardiopulmonary reflex; Exercise training; Female; Heart rate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000011

Variable positive end-expiratory pressure can maintain oxygenation in experimental acute respiratory distress syndrome induced by oleic acid in dogs
Provedor de dados: 56
Autores: Lanza,F.C.; Damasceno,M.C.P.; Leme,F.; Yagui,A.C.Z.; Paiva,K.C.; Luque,A.; Beppu,O.S.
The use of positive end-expiratory pressure (PEEP) or lung recruitment maneuvers (RM) to improve oxygenation in acute respiratory distress syndrome (ARDS) is used but it may reduce cardiac output (CO). Intermittent PEEP may avoid these complications. Our objective was to determine if variable PEEP compared with constant PEEP is capable of maintaining arterial oxygenation and minimizing hemodynamic alterations with or without RM. Eighteen dogs with ARDS induced by oleic acid were randomized into three equal groups: group 1, low variable PEEP; group 2, high variable PEEP, and group 3, RM + high variable PEEP. All groups were submitted to constant PEEP, followed by variable PEEP (PEEP was increased from 5 to 10 cmH2O in group 1, and from 5 to 18 cmH2O in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute respiratory distress syndrome (ARDS); PEEP; Mechanical ventilation; Oleic acid.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800007

Newborn screening for biotinidase deficiency in Brazil: biochemical and molecular characterizations
Provedor de dados: 56
Autores: C. Neto,E.; Schulte,J.; Rubim,R.; Lewis,E.; DeMari,J.; Castilhos,C.; Brites,A.; Giugliani,R.; Jensen,K.P.; Wolf,B.
Biotinidase deficiency is an inherited metabolic disorder characterized by neurological and cutaneous symptoms. Fortunately, it can be treated and the symptoms prevented by oral administration of the vitamin biotin. Using dried blood-soaked filter paper cards, biotinidase activity was determined in the sera of 225,136 newborns in Brazil. Mutation analysis performed on DNA from 21 babies with low serum biotinidase activity confirmed that 3 had profound biotinidase deficiency (less than 10% of mean normal sera biotinidase activity), 10 had partial biotinidase deficiency (10 to 30% of mean normal serum activity), 1 was homozygous for partial biotinidase deficiency, 4 were heterozygous for either profound or partial deficiency, and 3 were normal. Variability...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Newborn screening; Biotinidase deficiency; Biotinidase; Mutations; Enzyme assay.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300001

Structure and function of the selectin ligand PSGL-1
Provedor de dados: 56
Autores: Cummings,R.D.
P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric mucin-like 120-kDa glycoprotein on leukocyte surfaces that binds to P- and L-selectin and promotes cell adhesion in the inflammatory response. The extreme amino terminal extracellular domain of PSGL-1 is critical for these interactions, based on site-directed mutagenesis, blocking monoclonal antibodies, and biochemical analyses. The current hypothesis is that for high affinity interactions with P-selectin, PSGL-1 must contain O-glycans with a core-2 branched motif containing the sialyl Lewis x antigen (NeuAc&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;3Galß1&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;4[Fuc&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;1&lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-selectin; L-selectin; E-selectin; PSGL-1; O-glycosylation; Glycoprotein; Mucin; Tyrosine sulfate; Cell adhesion; Leukocytes; Neutrophils.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500004

Expression of TERT in precancerous gastric lesions compared to gastric cancer
Provedor de dados: 56
Autores: Duarte,M.C.; Babeto,E.; Leite,K.R.M.; Miyazaki,K.; Borim,A.A.; Rahal,P.; Silva,A.E.
The objective of this study was to determine the levels of TERT mRNA and TERT protein expression in stomach precancerous lesions such as intestinal metaplasia (IM) and gastric ulcer (GU) and compare them to gastric cancer (GC). Real-time PCR was performed to detect TERT mRNA expression levels in 35 biopsies of IM, 30 of GU, and 22 of GC and their respective normal mucosas. TERT protein was detected by immunohistochemistry in 68 samples, 34 of IM, 23 of GU, and 11 of GC. Increased TERT mRNA expression levels were observed in a significant number of cases, i.e., 46% of IM, 50% of GU, and 79% of GC. The relative mean level of TERT mRNA after normalization with the β-actin reference gene and comparison with the respective adjacent normal mucosa was slightly...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gastric ulcer; Intestinal metaplasia; Gastric cancer; TERT; Gene expression; Protein expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200003

The bradycardic and hypotensive responses to serotonin are reduced by activation of GABA A receptors in the nucleus tractus solitarius of awake rats
Provedor de dados: 56
Autores: Callera,J.C.; Colombari,E.; De Luca Jr.,L.A.; Menani,J.V.
We investigated the effects of bilateral injections of the GABA receptor agonists muscimol (GABA A) and baclofen (GABA B) into the nucleus tractus solitarius (NTS) on the bradycardia and hypotension induced by iv serotonin injections (5-HT, 2 µg/rat) in awake male Holtzman rats. 5-HT was injected in rats with stainless steel cannulas implanted bilaterally in the NTS, before and 5, 15, and 60 min after bilateral injections of muscimol or baclofen into the NTS. The responses to 5-HT were tested before and after the injection of atropine methyl bromide. Muscimol (50 pmol/50 nl, N = 8) into the NTS increased basal mean arterial pressure (MAP) from 115 ± 4 to 144 ± 6 mmHg, did not change basal heart rate (HR) and reduced the bradycardia (-40 ± 14 and -73 ± 26...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscimol; GABA receptors; Nucleus tractus solitarius; Parasympathetic component; Bezold-Jarisch reflex; Awake rats.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700016

Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection
Provedor de dados: 56
Autores: Corrêa-Oliveira,R.; Malaquias,L.C.C.; Falcão,P.L.; Viana,I.R.C.; Bahia-Oliveira,L.M.G.; Silveira,A.M.S.; Fraga,L.A.O.; Prata,A.; Coffman,R.L.; Lambertucci,J.R.; Cunha-Melo,J.R.; Martins-Filho,O.A.; Wilson,R.A.; Gazzinelli,G.
The role of different cytokines in the peripheral blood mononuclear cell (PBMC) proliferative response and in in vitro granuloma formation was evaluated in a cross-sectional study with patients with the different clinical forms and phases of Schistosoma mansoni infection, as well as a group of individuals "naturally" resistant to infection named normal endemic (NE). The blockage of IL-4 and IL-5 using anti-IL-4 and anti-IL-5 antibodies significantly reduced the PBMC proliferative response to soluble egg (SEA) and adult worm (SWAP) antigens in acute (ACT), chronic intestinal (INT) and hepatosplenic (HS) patients. Similar results were obtained in the in vitro granuloma formation. Blockage of IL-10 had no significant effect on either assay using PBMC from ACT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma mansoni human schistosomiasis mansoni cytokines IL-10 IL-4 IFN-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100024

Neuropsychological rehabilitation of memory deficits and activities of daily living in patients with Alzheimer's disease: a pilot study
Provedor de dados: 56
Autores: Ávila,R.; Bottino,C.M.C.; Carvalho,I.A.M.; Santos,C.B.; Seral,C.; Miotto,E.C.
Patients with Alzheimer's disease (AD) gradually lose their cognitive competence, particularly memory, and the ability to perform daily life tasks. Neuropsychological rehabilitation is used to improve cognitive functions by facilitating memory performance through the use of external aids and internal strategies. The effect of neuropsychological rehabilitation through memory training - motor movements, verbal association, and categorization - and activities of daily living (ADL) training was tested in a sample of 5 elderly out-patients (mean age: 77.4 ± 2.88 years), with mild AD (Mini-Mental State Examination score: 22.20 ± 2.17) and their caregivers. All patients had been taking rivastigmine (6-12 mg/day) for at least 3 months before being assigned to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alzheimer's disease treatment; Neuropsychological rehabilitation; Memory; Daily living activities.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100018

HLA-DR3 antigen in the resistance to idiopathic dilated cardiomyopathy
Provedor de dados: 56
Autores: Jin,B.; Wu,B.W.; Wen,Z.C.; Shi,H.M.; Zhu,J.
Idiopathic dilated cardiomyopathy (IDC) has been hypothesized as a multifactorial disorder initiated by an environment trigger in individuals with predisposing human leukocyte antigen (HLA) alleles. Published data on the association between HLA-DR3 antigen and IDC risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. Studies were identified by searching the PUBMED and Embase database (starting from June 2015). A total of 19 case-control studies including 1378 cases and 10383 controls provided data on the association between HLA-DR3 antigen and genetic susceptibility to IDC. Overall, significantly decreased frequency of HLA-DR3 allele (OR=0.72; 95%CI=0.58-0.90; P=0.004) was found in patients with IDC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-DR; Dilated cardiomyopathy; Polymorphism; Meta-analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400708

Bone mineral density of Brazilian girls with juvenile dermatomyositis
Provedor de dados: 56
Autores: Castro,T.C.M. de; Terreri,M.T.R.A.; Szejnfeld,V.L.; Len,C.; Fonseca,A.S.M. da; Hilário,M.O.E.
We measured bone mineral density (BMD) in girls with juvenile dermatomyositis (JDM) considering multiple factors in order to determine if it could be used as a predictor of reduction in bone mass. A cross-sectional study of lumbar spine BMD (L2-L4) was conducted on 10 girls aged 7-16 years with JDM. A group of 20 age-matched healthy girls was used as control. Lumbar spine BMD was measured by dual-energy X-ray absorptiometry. Weight, height and pubertal Tanner stage were determined in all patients and controls. Duration of disease and mean daily and cumulative steroid doses were calculated for all patients on the basis of their medical charts. JDM activity was determined on the basis of the presence of muscle weakness, cutaneous vasculitis and/or elevation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatomyositis; Adolescent girls; Bone mineral density; Osteoporosis; Corticosteroids.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200020

Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth
Provedor de dados: 56
Autores: Wen,J.; Li,H.T.; Li,S.H.; Li,X.; Duan,J.M.
Stem cells from human exfoliated deciduous teeth (SHEDs) have great potential to treat various dental-related diseases in regenerative medicine. They are usually maintained with 10% fetal bovine serum (FBS) in vitro. Modified platelet-rich plasma (mPRP) would be a safe alternative to 10% FBS during SHEDs culture. Therefore, our study aimed to compare the proliferation and differentiation of SHEDs cultured in mPRP and FBS medium to explore an optimal concentration of mPRP for SHEDs maintenance. Platelets were harvested by automatic blood cell analyzer and activated by repeated liquid nitrogen freezing and thawing. The platelet-related cytokines were examined and analyzed by ELISA. SHEDs were extracted and cultured with different concentrations of mPRP or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MPRP; SHEDs; ALP; RUNX2; OCN.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000604

Limited-sampling strategy models for estimating the pharmacokinetic parameters of 4-methylaminoantipyrine, an active metabolite of dipyrone
Provedor de dados: 56
Autores: Suarez-Kurtz,G.; Ribeiro,F.M.; Estrela,R.C.E.; Vicente,F.L.; Struchiner,C.J.
Bioanalytical data from a bioequivalence study were used to develop limited-sampling strategy (LSS) models for estimating the area under the plasma concentration versus time curve (AUC) and the peak plasma concentration (Cmax) of 4-methylaminoantipyrine (MAA), an active metabolite of dipyrone. Twelve healthy adult male volunteers received single 600 mg oral doses of dipyrone in two formulations at a 7-day interval in a randomized, crossover protocol. Plasma concentrations of MAA (N = 336), measured by HPLC, were used to develop LSS models. Linear regression analysis and a "jack-knife" validation procedure revealed that the AUC0-&lt;FONT FACE=Symbol&gt;¥&lt;/FONT&gt; and the Cmax of MAA can be accurately predicted (R²&gt;0.95, bias &lt;1.5%, precision...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dipyrone; 4-methylaminoantipyrine; Limited-sampling models; Pharmacokinetics; Bioequivalence.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100017

Food consumption by young adults living in Ribeirão Preto, SP, 2002/2004
Provedor de dados: 56
Autores: Molina,M.C.; Bettiol,H.; Barbieri,M.A; Silva,A.A.M.; Conceição,S.I.O.; Dos-Santos,J.E.
There is evidence showing a close relationship between diet and the occurrence of non-communicable chronic diseases. The present study assessed food consumption in a 2002/2004 cohort of young adults born in 1978/79 in Ribeirão Preto, SP, Brazil. The composition of the habitual diet consumed by a sample of 2063 individuals aged 23-25 years was analyzed using a validated semi-quantitative food frequency questionnaire based on studies of prevention of non-communicable chronic diseases. The Dietsys software was used for dietary calculations. In terms of WHO/2003 recommendations, there was a high mean daily consumption of energy from fat (consumption: 35.4%; recommendation: 15-30%), a low mean intake of energy from carbohydrates (47.5%; 55-75%) and a low mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food consumption; Food frequency questionnaire; Food groups; Feeding habits; Diet; Non-communicable diseases of adults.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900013

Dopaminergic profile of new heterocyclic N-phenylpiperazine derivatives
Provedor de dados: 56
Autores: Neves,G.; Fenner,R.; Heckler,A.P.; Viana,A.F.; Tasso,L.; Menegatti,R.; Fraga,C.A.M.; Barreiro,E.J.; Dalla-Costa,T.; Rates,S.M.K.
Dopamine constitutes about 80% of the content of central catecholamines and has a crucial role in the etiology of several neuropsychiatric disorders, including Parkinson's disease, depression and schizophrenia. Several dopaminergic drugs are used to treat these pathologies, but many problems are attributed to these therapies. Within this context, the search for new more efficient dopaminergic agents with less adverse effects represents a vast research field. The aim of the present study was to report the structural design of two N-phenylpiperazine derivatives, compound 4: 1-[1-(4-chlorophenyl)-1H-4-pyrazolylmethyl]-4-phenylhexahydropyrazine and compound 5: 1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-ylmethyl]-4-phenylhexahydropyrazine, planned to be dopamine...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: N-phenylpiperazine derivatives; Dopamine; Apomorphine hypothermia; Amphetamine stereotypy; Catalepsy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500010

Associations between CD36 gene polymorphisms and susceptibility to coronary artery heart disease
Provedor de dados: 56
Autores: Zhang,Y.; Ling,Z.Y.; Deng,S.B.; Du,H.A.; Yin,Y.H.; Yuan,J.; She,Q.; Chen,Y.Q.
Associations between polymorphisms of the CD36 gene and susceptibility to coronary artery heart disease (CHD) are not clear. We assessed allele frequencies and genotype distributions of CD36 gene polymorphisms in 112 CHD patients and 129 control patients using semi-quantitative polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. Additionally, we detected CD36 mRNA expression by real-time quantitative PCR, and we quantified plasma levels of oxidized low-density lipoprotein (ox-LDL) using an enzyme-linked immunosorbent assay (ELISA). There were no significant differences between the two groups (P&gt;0.05) in allele frequencies of rs1761667 or in genotype distribution and allele frequencies of rs3173798. The genotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary artery disease; CD36; Rs1761667; Rs3173798; Single nucleotide polymorphism; Ox-LDL.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000895

Dose-response effects of systemic anandamide administration in mice sequentially submitted to the open field and elevated plus-maze tests
Provedor de dados: 56
Autores: Ribeiro,A.; Ferraz-de-Paula,V.; Pinheiro,M.L.; Palermo-Neto,J.
The endocannabinoid system is involved in the control of many physiological functions, including the control of emotional states. In rodents, previous exposure to an open field increases the anxiety-like behavior in the elevated plus-maze. Anxiolytic-like effects of pharmacological compounds that increase endocannabinoid levels have been well documented. However, these effects are more evident in animals with high anxiety levels. Several studies have described characteristic inverted U-shaped dose-response effects of drugs that modulate the endocannabinoid levels. However, there are no studies showing the effects of different doses of exogenous anandamide, an endocannabinoid, in animal models of anxiety. Thus, in the present study, we determined the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Endocannabinoid; Anandamide; Anxiety; Open field; Elevated plus-maze.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600013

Oxytocin is a cardiovascular hormone
Provedor de dados: 56
Autores: Gutkowska,J.; Jankowski,M.; Mukaddam-Daher,S.; McCann,S.M.
Oxytocin (OT), a nonapeptide, was the first hormone to have its biological activities established and chemical structure determined. It was believed that OT is released from hypothalamic nerve terminals of the posterior hypophysis into the circulation where it stimulates uterine contractions during parturition, and milk ejection during lactation. However, equivalent concentrations of OT were found in the male hypophysis, and similar stimuli of OT release were determined for both sexes, suggesting other physiological functions. Indeed, recent studies indicate that OT is involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of cardiovascular functions. It has long been known that OT induces...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxytocin; Natriuretic peptides; Heart.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600003

Electrolocation-communication discharges of the fish Gymnotus carapo L. (Gymnotidae: Gymnotiformes) during behavioral sleep
Provedor de dados: 56
Autores: Stopa,R.M.; Hoshino,K.
Technical problems have hampered the study of sleep in teleosts. The electrical discharges of Gymnotus carapo L. (Gymnotidae: Gymnotiformes) were monitored to evaluate their ease and reliability as parameters to study sleep. The discharges were detected by electrodes immersed in a glass aquarium and were recorded on a conventional polygraph. G. carapo showed conspicuous signs of behavioral sleep. During these periods, opercular beat rates were counted, electric discharges recorded, and the "sharp discharge increase" (SDI) of the orienting reflex was investigated. All 20 animals monitored maintained electrical discharges during behavioral sleep. The discharge frequencies during sleep (50.3 ± 10.4 Hz) were not significantly different from those observed when...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sleep; Fish; Electric discharges; Cannibalism; Antipredation mechanism.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000008

Partial purification of tightly bound mitochondrial hexokinase from maize (Zea mays L.) root membranes
Provedor de dados: 56
Autores: Rezende,G.L.; Logullo,C.; Meyer,L.; Machado,L.B.; Oliveira-Carvalho,A.L.; Zingali,R.B.; Cifuentes,D.; Galina,A.
In mammals, hexokinase (HK) is strategically located at the outer membrane of mitochondria bound to the porin protein. The mitochondrial HK is a crucial modulator of apoptosis and reactive oxygen species generation. In plants, these properties related to HK are unknown. In order to better understand the physiological role of non-cytosolic hexokinase (NC-HK) in plants, we developed a purification strategy here described. Crude extract of 400 g of maize roots (230 mg protein) contained a specific activity of 0.042 µmol G6P min-1 mg PTN-1. After solubilization with detergent two fractions were obtained by DEAE column chromatography, NC-HK 1 (specific activity = 3.6 µmol G6P min-1 mg PTN-1 and protein recovered = 0.7 mg) and NC-HK 2. A major purification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrophobic interaction chromatography; Maize; Mitochondrial hexokinase; Non-cytosolic hexokinase; Purification.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900003

Modeling the pressure inactivation dynamics of Escherichia coli
Provedor de dados: 56
Autores: Yamamoto,K.; Matsubara,M.; Kawasaki,S.; Bari,M.L.; Kawamoto,S.
Escherichia coli, as a model microorganism, was treated in phosphate-buffered saline under high hydrostatic pressure between 100 and 300 MPa, and the inactivation dynamics was investigated from the viewpoint of predictive microbiology. Inactivation data were curve fitted by typical predictive models: logistic, Gompertz and Weibull functions. Weibull function described the inactivation curve the best. Two parameters of Weibull function were calculated for each holding pressure and their dependence on holding pressure was obtained by interpolation. With the interpolated parameters, inactivation curves were simulated and compared with the experimental data sets.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Predictive microbiology; Pressure; Escherichia coli; Inactivation; Model.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800014

Cytokines as determinants of disease and disease interactions
Provedor de dados: 56
Autores: Sher,A.; Gazzinelli,R.T.; Jankovic,D.; Scharton-Kersten,T.; Yap,G.; Doherty,T.M.; Wynn,T.
The immune response to pathogens results in both host resistance and immunopathology. Cytokines and in particular those lymphokines produced by Th1 and Th2 cells play a key role in determining the balance between these two immunologic outcomes. Recent data suggest that interleukin-10, a product of both Th2 cells and macrophages, protects the host against excessive immunopathology. The cytokine environment generated by different pathogens may also influence the course and outcome of infections with unrelated organisms. This relationship may be particularly important in the case of HIV-1 where prior Th1 or Th2 biases established by helminth or intracellular infections may influence either initial viral susceptibility or drive progression to AIDS through...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines disease immunopathology HIV-I parasites; Interleukin-10.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100011

Distribution of HCV genotypes among different exposure categories in Brazil
Provedor de dados: 56
Autores: Oliveira,M.L.A.; Bastos,F.I.; Sabino,R.R.; Paetzold,U.; Schreier,E.; Pauli,G.; Yoshida,C.F.T.
Hepatitis C virus (HCV) infection is widespread and responsible for more than 60% of chronic hepatitis cases. HCV presents a genetic variability which has led to viral classification into at least 6 genotypes and a series of subtypes. These variants present characteristic geographical distribution, but their association with different responses to treatment with interferon and severity of disease still remains controversial. The aim of this study was to investigate the patterns of distribution of HCV genotypes among different exposure categories in Brazil. Two hundred and fifty anti-HCV positive samples were submitted to HCV-RNA detection by RT-PCR and their genotype was determined by restriction fragment length polymorphism (RFLP) analysis. In addition,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HCV; HCV genotypes; Brazil.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300005

Genetic association of SNPs in the FTO gene and predisposition to obesity in Malaysian Malays
Provedor de dados: 56
Autores: Apalasamy,Y.D.; Ming,M.F.; Rampal,S.; Bulgiba,A.; Mohamed,Z.
The common variants in the fat mass- and obesity-associated (FTO) gene have been previously found to be associated with obesity in various adult populations. The objective of the present study was to investigate whether the single nucleotide polymorphisms (SNPs) and linkage disequilibrium (LD) blocks in various regions of the FTO gene are associated with predisposition to obesity in Malaysian Malays. Thirty-one FTO SNPs were genotyped in 587 (158 obese and 429 non-obese) Malaysian Malay subjects. Obesity traits and lipid profiles were measured and single-marker association testing, LD testing, and haplotype association analysis were performed. LD analysis of the FTO SNPs revealed the presence of 57 regions with complete LD (D’ = 1.0). In addition, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FTO gene; SNP; Obesity; Linkage disequilibrium; Haplotypes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200003

Concentration of CCL11, CXCL8 and TNF-alpha in sputum and plasma of patients undergoing asthma or chronic obstructive pulmonary disease exacerbation
Provedor de dados: 56
Autores: Daldegan,M.B.; Teixeira,M.M.; Talvani,A.
Asthma and chronic obstructive pulmonary disease (COPD) are common respiratory illnesses characterized by chronic inflammation of the airways. The characterization of induced or spontaneously produced sputum is a useful technique to assess airway inflammation. In the present study, we compared the concentrations of CCL2, CCL11, CXCL8, and tumor necrosis factor-alpha (TNF-alpha) in plasma and induced sputum of patients with severe asthma or COPD and correlated the levels of these mediators with inflammatory cells in sputum. Asthmatic patients had elevated levels of eosinophils (40.1 ± 6.24%) in sputum whereas neutrophils (63.3 ± 4.66%) predominated in COPD patients. The levels of the chemokine CCL11 were markedly increased in sputum (708.7 ± 330.7 pg/ml)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Chronic obstructive pulmonary disease; Chemokines; TNF-alpha.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900010

In vivo antithrombotic properties of a heparin from the oocyte test cells of the sea squirt Styela plicata(Chordata-Tunicata)
Provedor de dados: 56
Autores: Cardilo-Reis,L.; Cavalcante,M.C.M.; Silveira,C.B.M.; Pavão,M.S.G.
In the ascidian Styela plicata, the oocytes are surrounded by two types of accessory cells named follicle cells and test cells. A heparin-like substance with an anticoagulant activity equivalent to 10% of mammalian heparin and about 5% as potent as the mammalian counterpart for the inhibition of thrombin by antithrombin was isolated from the oocyte test cells. In the present study, we compared the antithrombotic and hemorrhagic effects of sea squirt oocyte test cell heparin with those of porcine heparin in rat models of venous thrombosis and blood loss. Intravenous administration of the oocyte test cell heparin to Wistar rats (both sexes, weighing ~300 g, N = 4 in each group) at a dose of 5.0 mg/kg body weight, which produced a 1.8-fold increase in plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascidian; Heparin; Experimental thrombosis; Antithrombotics; Hemorrhage.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100004

The growing competition in Brazilian science: rites of passage, stress and burnout
Provedor de dados: 56
Autores: de Meis,L.; Velloso,A.; Lannes,D.; Carmo,M.S.; de Meis,C.
Brazil's scientific community is under pressure. Each year there is an increase in its contribution to international science and in the number of students who are trained to do research and teach at an advanced level. Most of these activities are carried out in state and federal universities, but with government funding that has decreased by more than 70% since 1996. Interviews with graduate students, post-doctoral fellows and professors in one university department with a strong research tradition illustrate the level of stress engendered by the conflict between increasing competition and diminishing resources, and serve to underscore the negative effects on creativity and on the tendency to choose science as a career.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Science funding; Public and private universities; Mental suffering; Publish or perish; Academic pressure; MSc and PhD students.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900001

Time course of training-induced microcirculatory changes and of vegf expression in skeletal muscles of spontaneously hypertensive female rats
Provedor de dados: 56
Autores: Amaral,S.L.; Sanchez,L.S.; Chang,A.J.B.A.; Rossoni,L.V.; Michelini,L.C.
Exercise-induced vessel changes modulate arterial pressure (AP) in male spontaneously hypertensive rats (SHR). Vascular endothelial growth factor (VEGF) is important for angiogenesis of skeletal muscle. The present study evaluated the time course of VEGF and angiogenesis after short- and long-term exercise training of female SHR and Wistar Kyoto (WKY) rats, 8-9 weeks (200-250 g). Rats were allocated to daily training or remained sedentary for 3 days (N = 23) or 13 weeks (N = 23). After training, the carotid artery was catheterized for AP measurements. Locomotor (tibialis anterior and gracilis) and non-locomotor skeletal muscles (temporalis) were harvested and prepared for histologic and protein expression analyses. Training increased treadmill performance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High blood pressure; Exercise training; Capillary density; VEGF expression; Skeletal muscle angiogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500012

Role of endothelium in angiotensin II formation by the rat aorta and mesenteric arterial bed
Provedor de dados: 56
We investigated the angiotensin II (Ang II)-generating system by analyzing the vasoconstrictor effect of Ang II, angiotensin I (Ang I), and tetradecapeptide (TDP) renin substrate in the absence and presence of inhibitors of the renin-angiotensin system in isolated rat aortic rings and mesenteric arterial beds with and without functional endothelium. Ang II, Ang I, and TDP elicited a dose-dependent vasoconstrictor effect in both vascular preparations that was completely blocked by the Ang II receptor antagonist saralasin (50 nM). The angiotensin converting enzyme (ACE) inhibitor captopril (36 µM) completely inhibited the vasoconstrictor effect elicited by Ang I and TDP in aortic rings without affecting that of Ang II. In contrast, captopril (36 µM)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin II; Angiotensin converting enzyme; Renin-angiotensin system; Endothelium; Blood vessels; Captopril.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500013

Immunologic patterns associated with cure in human American cutaneous leishmaniasis
Provedor de dados: 56
Autores: Coutinho,S.G.; Da-Cruz,A.M.; Bertho,A.L.; Santiago,M.A.; De-Luca,P.
Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were performed. Antigen-stimulated cells were harvested for CD4 and CD8 phenotype analysis and the levels of gamma interferon (IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;) and interleukin 4 (IL-4) produced were also determined in the culture supernatants. Two different patterns of Lb-induced T cell responses were observed: a) predominance of responding CD4+ cells and mixed type 1 and type 2 cytokine production (IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; and IL-4) during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human cutaneous leishmaniasis Leishmania braziliensis CD4+ and CD8+ cells IFN-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt; IL-4.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019

Epidemiology of recurrent venous thrombosis
Provedor de dados: 56
Autores: Ribeiro,D.D.; Lijfering,W.M.; Barreto,S.M.; Rosendaal,F.R.; Rezende,S.M.
Venous thrombosis, including deep vein thrombosis and pulmonary embolism, is a common disease that frequently recurs. Recurrence can be prevented by anticoagulants, but this comes at the risk of bleeding. Therefore, assessment of the risk of recurrence is important to balance the risks and benefits of anticoagulant treatment. This review briefly outlines what is currently known about the epidemiology of recurrent venous thrombosis, and focuses in more detail on potential new risk factors for venous recurrence. The general implications of these findings in patient management are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recurrent venous thrombosis; Epidemiology; Risk factors.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100001

Effect of one-week ethanol treatment on monoamine levels and dopaminergic receptors in rat striatum
Provedor de dados: 56
Autores: Vasconcelos,S.M.M.; Macêdo,D.S.; Lima,L.O.; Sousa,F.C.F.; Fonteles,M.M.F.; Viana,G.S.B.
We studied the effects of ethanol on the levels of norepinephrine, dopamine, serotonin (5-HT) and their metabolites as well as on D1- and D2-like receptors in the rat striatum. Ethanol (2 or 4 g/kg, po) was administered daily by gavage to male Wistar rats and on the 7th day, 30 min or 48 h after drug administration, the striatum was dissected for biochemical assays. Monoamine and metabolite concentrations were measured by HPLC and D1- and D2-like receptor densities were determined by binding assays. Scatchard analyses showed decreases of 30 and 43%, respectively, in D1- and D2-like receptor densities and no change in dissociation constants (Kd) 48 h after the withdrawal of the dose of 4 g/kg. Ethanol, 2 g/kg, was effective only on the density of D2-like...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat striatum; Dopamine; Serotonin; Alcohol ingestion; Noradrenaline; D1 and D2 receptor density; Kd.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400013

p19Arf sensitizes B16 melanoma cells to interferon-β delivered via mesenchymal stem cells in vitro
Provedor de dados: 56
Autores: Da-Costa,R.C.; Vieira,I.L.; Hunger,A.; Tamura,R.E.; Strauss,B.E.
The immune stimulatory and anti-neoplastic functions of type I interferon have long been applied for the treatment of melanoma. However, the systemic application of high levels of this recombinant protein is often met with toxicity. An approach that provides localized, yet transient, production of type I interferon may overcome this limitation. We propose that the use of mesenchymal stem cells (MSCs) as delivery vehicles for the production of interferon-β (IFNβ) may be beneficial when applied together with our cancer gene therapy approach. In our previous studies, we have shown that adenovirus-mediated gene therapy with IFNβ was especially effective in combination with p19Arf gene transfer, resulting in immunogenic cell death. Here we showed that MSCs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene therapy; Immunotherapy; Stem cell therapy; Adenovirus; P53; Interferon-β.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300601

Biomechanics and structural adaptations of the rat femur after hindlimb suspension and treadmill running
Provedor de dados: 56
Autores: Shimano,M.M.; Volpon,J.B.
We microscopically and mechanically evaluated the femurs of rats subjected to hindlimb unloading (tail suspension) followed by treadmill training. Female Wistar rats were randomly divided into five groups containing 12-14 rats: control I (118 days old), control II (139 days old), suspended (tail suspension for 28 days), suspended-released (released for 21 days after 28 days of suspension), and suspended-trained (trained for 21 days after 28 days of suspension). We measured bone resistance by bending-compression mechanical tests of the entire proximal half of the femur and three-point bending tests of diaphyseal cortical bone. We determined bone microstructure by tetracycline labeling of trabecular and cortical bone. We found that tail suspension weakened...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hindlimb suspension; Exercise; Biomechanics; Femur; Rat.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400004

The control of deliberate waiting strategies in a stop-signal task
Provedor de dados: 56
Autores: Sylwan,R.P.
To inhibit an ongoing flow of thoughts or actions has been largely considered to be a crucial executive function, and the stop-signal paradigm makes inhibitory control measurable. Stop-signal tasks usually combine two concurrent tasks, i.e., manual responses to a primary task (go-task) are occasionally countermanded by a stimulus which signals participants to inhibit their response in that trial (stop-task). Participants are always instructed not to wait for the stop-signal, since waiting strategies cause the response times to be unstable, invalidating the data. The aim of the present study was to experimentally control the strategies of waiting deliberately for the stop-signal in a stop-task by means of an algorithm that measured the variation in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention; Stop-task; Reaction time; Vision; Executive functions; Laterality.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600011

Antioxidant properties of natural compounds used in popular medicine for gastric ulcers
Provedor de dados: 56
Autores: Repetto,M.G.; Llesuy,S.F.
There is evidence concerning the participation of reactive oxygen species in the etiology and physiopathology of human diseases, such as neurodegenerative disorders, inflammation, viral infections, autoimmune pathologies, and digestive system disorders such as gastrointestinal inflammation and gastric ulcer. The role of these reactive oxygen species in several diseases and the potential antioxidant protective effect of natural compounds on affected tissues are topics of high current interest. To consider a natural compound or a drug as an antioxidant substance it is necessary to investigate its antioxidant properties in vitro and then to evaluate its antioxidant functions in biological systems. In this review article, we shall consider the role of natural...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Free radicals; Natural antioxidants; Gastric ulcers; Artemisia douglasiana Besser; Dehydroleucodine.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500003

Anti-inflammatory and antispasmodic activity of Ipomoea imperati (Vahl) Griseb (Convolvulaceae)
Provedor de dados: 56
Autores: Paula,A.C.B.; Hayashi,L.S.S.; Freitas,J.C.
Ipomoea imperati (Convolvulaceae) lives on the sandy shores of the Brazilian coast and in other areas of the world. The anti-inflammatory activity of a methanol-water extract of the leaves of I. imperati was investigated in experimental models of acute and subchronic inflammation. Topical application of the extract (10 mg/ear) inhibited mouse ear edema induced by croton oil (89.0 ± 1.3% by the lipid fraction with an IC50 of 3.97 mg/ear and 57.0 ± 1.3% by the aqueous fraction with an IC50 of 3.5 mg/ear) and arachidonic acid (42.0 ± 2.0% with an IC50 of 4.98 mg/ear and 31.0 ± 2.0% with an IC50 of 4.72 mg/ear). Phospholipase A2, purified from Apis mellifera bee venom, was also inhibited by the extract (5.0 mg/ml lipid and aqueous fraction) in vitro in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-inflammatory activity; Antispasmodic activity; Arachidonic acid; Croton oil; Medicinal plants; Phospholipase A2.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100014

The APOB rs693 polymorphism impacts the lipid profile of Brazilian older adults
Provedor de dados: 56
Autores: Alves,E.S.; Henriques,A.D.; Tonet-Furioso,A.C.; Paula,R.S.; Gomes,L.O.; Moraes,C.F.; Nóbrega,O.T.
The apolipoprotein B (APOB) gene contains several polymorphic sites described as risk modifiers for cardiovascular events. The objective of this study was to verify the association of the classic APOB Xba I polymorphism (rs693) with atherosclerotic risk factors in a segment of the Brazilian elderly population considering their usual dietary intake. Clinical and biochemical characteristics as well as total caloric and fat intake data were determined from 644 elderly individuals. Polymorphism analysis was performed by conventional polymerase chain reaction followed by enzyme restriction. Statistical analyses compared measures and proportions according to different APOB genotypic combinations. Statistically significant association was found between Xba I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein B; Genetic polymorphism; Elderly; Hyperlipidemia; Diet.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300608

Antipyretic and antioxidant activities of 5-trifluoromethyl-4,5-dihydro-1H-pyrazoles in rats
Provedor de dados: 56
Autores: Pasin,J.S.M.; Ferreira,A.P.O.; Saraiva,A.L.L.; Ratzlaff,V.; Andrighetto,R.; Machado,P.; Marchesan,S.; Zanette,R.A.; Bonacorso,H.G.; Zanatta,N.; Martins,M.A.P.; Ferreira,J.; Mello,C.F.
The objective of this study was to determine the effect of eight 5-hydroxy-5-trifluoromethyl-4,5-dihydro-1H-1-carboxyamidepyrazoles (TFDPs) on rat body temperature and baker’s yeast-induced fever. TFDPs or vehicle (5% Tween 80 in 0.9% NaCl, 5 mL/kg) were injected subcutaneously and rectal temperature was measured as a function of time in 28-day-old male Wistar rats (N = 5-12 per group). Antipyretic activity was determined in feverish animals injected with baker’s yeast (Saccharomyces cerevisiae suspension, 0.135 mg/kg, 10 mL/kg, ip). 3-Ethyl- and 3-propyl-TFDP (140 and 200 μmol/kg, respectively, 4 h after yeast injection) attenuated baker’s yeast-induced fever by 61 and 82%, respectively. These two effective antipyretics were selected for subsequent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrazole derivatives; Fever; Cytokine levels; Cyclooxygenase.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200009

Clinicopathological significance of PTPN12 expression in human breast cancer
Provedor de dados: 56
Autores: Xunyi,Yuan; Zhentao,Yuan; Dandan,Jiang; Funian,Li.
Protein tyrosine phosphatase non-receptor type 12 (PTPN12) is a recently identified tumor suppressor gene (TSG) that is frequently compromised in human triple-negative breast cancer. In the present study, we investigated the expression of PTPN12 protein by patients with breast cancer in a Chinese population and the relationship between PTPN12 expression levels and patient clinicopathological features and prognosis. Additionally, we explored the underlying down-regulation mechanism from the perspective of an epigenetic alteration. We examined PTPN12 mRNA expression in five breast cancer cell lines using semi-quantitative reverse-transcription PCR, and detected PTPN12 protein expression using immunohistochemistry in 150 primary invasive breast cancer cases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Tumor suppressor gene; PTPN12; Prognosis; Methylation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200033

Changes in NAD/ADP-ribose metabolism in rectal cancer
Provedor de dados: 56
Autores: Yalcintepe,L.; Turker-Sener,L.; Sener,A.; Yetkin,G.; Tiryaki,D.; Bermek,E.
The extent of ADP-ribosylation in rectal cancer was compared to that of the corresponding normal rectal tissue. Twenty rectal tissue fragments were collected during surgery from patients diagnosed as having rectal cancer on the basis of pathology results. The levels of ADP-ribosylation in rectum cancer tissue samples (95.9 ± 22.1 nmol/ml) was significantly higher than in normal tissues (11.4 ± 4 nmol/ml). The level of NAD+ glycohydrolase and ADP-ribosyl cyclase activities in rectal cancer and normal tissue samples were measured. Cancer tissues had significantly higher NAD+ glycohydrolase and ADP-ribosyl cyclase activities than the control tissues (43.3 ± 9.1 vs 29.2 ± 5.2 and 6.2 ± 1.6 vs 1.6 ± 0.4 nmol mg-1 min-1). Approximately 75% of the NAD+...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rectal cancer; ADP-ribosylation; NAD+; NAD+ glycohydrolase; Poly(ADP-ribose) polymerase.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300006

Sentinel node biopsy in breast cancer: results in a large series
Provedor de dados: 56
Autores: Carvalho,S.M.T.; Mourão Netto,M.; Lima,E.N.P.; Pimentel,A.M.; Makdissi,F.B.; Osório,C.A.B.T.; Maciel,M.S.; Iyeyasu,H.; Collins,J.B.; Fontes,C.M.; Perina,A.L.; Soares,F.A.
Sentinel lymph node biopsy (SLNB) is an appropriate method for the evaluation of axillary status in cases of early breast cancer. We report our experience in treating cases evaluated using SLNB. We analyzed a total of 1192 cases assessed by means of SLNB from July 1999 to December 2007. SLNB processing was successfully completed in 1154 cases with the use of blue dye or radiolabeled 99mTc-Dextran-500, or both. Of these 1154 patients, 857 were N0(i-) (no regional lymph node metastasis, negative immunohistochemistry, IHC), 96 were N0(i+) (no regional lymph node metastasis histologically, positive IHC, no IHC cluster greater than 0.2 mm) and 201 were N1mi (greater than 0.2 mm, none greater than 2.0 mm). Most of the tumors (70%) were invasive ductal carcinomas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast neoplasms; Sentinel lymph node; Sentinel lymph node biopsy; Completion axillary dissection.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600011

Ethnicity as a risk factor for obstructive sleep apnea: comparison of Japanese descendants and white males in São Paulo, Brazil
Provedor de dados: 56
Autores: Genta,P.R.; Marcondes,B.F.; Danzi,N.J.; Lorenzi-Filho,G.
Some studies showed that Asians with obstructive sleep apnea (OSA) are thinner than Caucasians. Because obesity is a major risk factor for OSA, it was concluded that Asians are predisposed to OSA. However, body fat composition varies for a same body mass index (BMI) according to ethnicity. We firstly compared anthropometric characteristics, symptoms and associated disorders in all consecutive male Japanese descendants and white males with OSA referred for polysomnography. In a second analysis, all Japanese descendants were compared to a subgroup of white males, matched for apnea/hypopnea index and age. In the first analysis, age, symptoms, OSA severity and co-morbidities were similar among Japanese descendants (N = 54) and white patients (N = 466)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asians; Obesity; Obstructive sleep apnea; Japanese; Ethnicity; Body mass index.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800015

Effect of a rosmarinic acid supplemented hemodialysis fluid on inflammation of human vascular endothelial cells
Provedor de dados: 56
Autores: Wang,W-J.; Cheng,M-H.; Lin,J-H.; Weng,C-S.
Chronic systemic inflammation and repetitive damage of vascular endothelia by incompatible dialysis system are probable causes of cardiovascular disease in patients on dialysis. The present study aimed to assess in vitro biocompatibility and anti-inflammatory effect of hemodialysis fluid supplemented with rosmarinic acid (RA) using human umbilical vein endothelial cells (HUVEC). HUVECs (5×106 cells/mL) were pre-exposed to 1 μg/mL of lipopolysaccharides (LPS) and incubated with RA-supplemented hemodialysis fluid (HDF). Cytotoxicity was assessed qualitatively by morphologic assessment and quantitatively by MTT assay. Expressions of proinflammatory mediators were assessed using quantitative real-time PCR and production of NO was quantified. Phosphorylation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosmarinic acid; Hemodialysis fluid; Anti-inflammation; Endothelial cells; LPS.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200606

Effects of estradiol benzoate on 5'-iodothyronine deiodinase activities in female rat anterior pituitary gland, liver and thyroid gland
Provedor de dados: 56
Autores: Lisbôa,P.C.; Curty,F.H.; Moreira,R.M.; Pazos-Moura,C.C.
There is little information on the possible effects of estrogen on the activity of 5'-deiodinase (5'-ID), an enzyme responsible for the generation of T3, the biologically active thyroid hormone. In the present study, anterior pituitary sonicates or hepatic and thyroid microsomes from ovariectomized (OVX) rats treated or not with estradiol benzoate (EB, 0.7 or 14 µg/100 g body weight, sc, for 10 days) were assayed for type I 5'-ID (5'-ID-I) and type II 5'-ID (5'-ID-II, only in pituitary) activities. The 5'-ID activity was evaluated by the release of 125I from deiodinated 125I rT3, using specific assay conditions for type I or type II. Serum TSH and free T3 and free T4 were measured by radioimmunoassay. OVX alone induced a reduction in pituitary 5'-ID-I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5'-iodothyronine deiodinase; Ovariectomy; Estradiol; Triiodothyronine; Thyroxine; Thyrotropin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200016

Effect of three exercise programs on patients with chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Dourado,V.Z.; Tanni,S.E.; Antunes,L.C.O.; Paiva,S.A.R.; Campana,A.O.; Renno,A.C.M.; Godoy,I.
We compared the effect of three different exercise programs on patients with chronic obstructive pulmonary disease including strength training at 50_80% of one-repetition maximum (1-RM) (ST; N = 11), low-intensity general training (LGT; N = 13), or combined training groups (CT; N = 11). Body composition, muscle strength, treadmill endurance test (TEnd), 6-min walk test (6MWT), Saint George's Respiratory Questionnaire (SGRQ), and baseline dyspnea (BDI) were assessed prior to and after the training programs (12 weeks). The training modalities showed similar improvements (P &gt; 0.05) in SGRQ-total (ST = 13 ± 14%; CT = 12 ± 14%; LGT = 11 ± 10%), BDI (ST = 1.8 ± 4; CT = 1.8 ± 3; LGT = 1 ± 2), 6MWT (ST = 43 ± 51 m; CT = 48 ± 50 m; LGT = 31 ± 75 m), and TEnd (ST...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Exercises; Rehabilitation; Strength training.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300007

Involvement of the TRPV1 channel in the modulation of spontaneous locomotor activity, physical performance and physical exercise-induced physiological responses
Provedor de dados: 56
Autores: Hudson,A.S.R.; Kunstetter,A.C.; Damasceno,W.C.; Wanner,S.P.
Physical exercise triggers coordinated physiological responses to meet the augmented metabolic demand of contracting muscles. To provide adequate responses, the brain must receive sensory information about the physiological status of peripheral tissues and organs, such as changes in osmolality, temperature and pH. Most of the receptors involved in these afferent pathways express ion channels, including transient receptor potential (TRP) channels, which are usually activated by more than one type of stimulus and are therefore considered polymodal receptors. Among these TRP channels, the TRPV1 channel (transient receptor potential vanilloid type 1 or capsaicin receptor) has well-documented functions in the modulation of pain sensation and thermoregulatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Afferent; Capsaicin; Hyperthermia; Metabolism; Physical activity; Thermoregulation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600301

Fast and slow voltage modulation of apical Cl- permeability in toad skin at high [K+]
Provedor de dados: 56
Autores: Procopio,J.
The influence of voltage on the conductance of toad skin was studied to identify the time course of the activation/deactivation dynamics of voltage-dependent Cl- channels located in the apical membrane of mitochondrion-rich cells in this tissue. Positive apical voltage induced an important conductance inhibition which took a few seconds to fully develop and was instantaneously released by pulse inversion to negative voltage, indicating a short-duration memory of the inhibiting factors. Sinusoidal stimulation at 23.4 mM [Cl-] showed hysteresis in the current versus voltage curves, even at very low frequency, suggesting that the rate of voltage application was also relevant for the inhibition/releasing effect to develop. We conclude that the voltage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cl- channel; Toad skin; Voltage modulation; Epithelia.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800018

Implications of extubation failure and prolonged mechanical ventilation in the postoperative period following elective intracranial surgery
Provedor de dados: 56
Autores: Vidotto,M.C.; Sogame,L.C.; Gazzotti,M.R.; Prandini,M.; Jardim,J.R.
Patients undergoing neurosurgery are predisposed to a variety of complications related to mechanical ventilation (MV). There is an increased incidence of extubation failure, pneumonia, and prolonged MV among such patients. The aim of the present study was to assess the influence of extubation failure and prolonged MV on the following variables: postoperative pulmonary complications (PPC), mortality, reoperation, tracheostomy, and duration of postoperative hospitalization following elective intra-cranial surgery. The study involved a prospective observational cohort of 317 patients submitted to elective intracranial surgery for tumors, aneurysms and arteriovenous malformation. Preoperative assessment was performed and patients were followed up for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurosurgery; Craniotomy; Ventilator weaning; Intratracheal intubation; Postoperative period.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200014

Anti-inflammatory and antinociceptive activities of an acid fraction of the seeds of Carpotroche brasiliensis (Raddi) (Flacourtiaceae)
Provedor de dados: 56
Autores: Lima,J.A.; Oliveira,A.S.; Miranda,A.L.P. de; Rezende,C.M.; Pinto,A.C.
Carpotroche brasiliensis is a native Brazilian tree belonging to the Oncobeae tribe of Flacourtiaceae. The oil extracted from its seeds contains as major constituents the same cyclopentenyl fatty acids hydnocarpic (40.5%), chaulmoogric (14.0%) and gorlic (16.1%) acids found in the better known chaulmoogra oil prepared from the seeds of various species of Hydnocarpus (Flacourtiaceae). These acids are known to be related to the pharmacological activities of these plants and to their use as anti-leprotic agents. Although C. brasiliensis oil has been used in the treatment of leprosy, a disease that elicits inflammatory responses, the anti-inflammatory and analgesic activities of the oil and its constituents have never been characterized. We describe the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carpotroche brasiliensis; Flacourtiaceae; Cyclopentenyl fatty acids; Anti-inflammatory effec; Analgesic; Antinociceptive effect.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700013

Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase
Provedor de dados: 56
Autores: Diaz,R.S.; Sabino,E.C.
For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that would survive the high temperatures needed for DNA denaturation. The development of enzymes with an inherent 3' to 5' exonuclease proofreading activity, lacking in Taq polymerase, would be an improvement when higher fidelity is needed. We used the forward mutation assay to compare the fidelity of Taq polymerase and Thermotoga maritima (ULTMA™) DNA polymerase, an enzyme that does have proofreading activity. We did not find significant differences in the fidelity of either enzyme, even when using optimal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Polymerase chain reaction fidelity; Thermus aquaticus (Taq) DNA polymerase; Thermotoga maritima DNA polymerase.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000001

Gastric emptying of liquids in rats dehydrated by water deprivation
Provedor de dados: 56
Autores: Baracat,E.C.E.; Collares,E.F.
The gastric emptying of liquids was investigated in male Wistar rats (8 to 10 weeks old, 210-300 g) dehydrated by water deprivation. In this model of dehydration, weight loss, hematocrit and plasma density were significantly higher in the dehydrated animals than in the control groups after 48 and 72 h of water deprivation (P&amp;lt;0.05). Three test meals (saline (N = 10), water (N = 10) and a WHO rehydrating solution containing in one liter 90 mEq sodium, 20 mEq potassium, 80 mEq chloride and 30 mEq citrate (N = 10)) were used to study gastric emptying following water deprivation for 24, 48 and 72 h. After 72 h, gastric emptying of the water (39.4% retention) and rehydrating solution (49.2% retention) test meals was significantly retarded compared to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dehydration; Water deprivation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100017

The role of K121Q ENPP1 polymorphism in diabetes mellitus and its complications
Provedor de dados: 56
Autores: Leitão,C.B.; Nabinger,G.B.; Krahe,A.L.; Bolson,P.B.; Gerchman,F.; Friedman,R.; Gross,J.L.; Canani,L.H.
The aim of the present study was to analyze the frequency of K121Q polymorphism in the ENPP1 gene of Brazilian subjects according to ethnic origin and to determine its possible association with diabetes mellitus (DM) and/or diabetic complications. A cross-sectional study was conducted on 1027 type 2 DM patients and 240 anonymous blood donors (BD). Ethnicity was classified based on self-report of European and African descent. The Q allele frequency was increased in African descendant type 2 DM patients (KK = 25.9%, KQ = 48.2%, and QQ = 25.9%) and BD (KK = 22.0%, KQ = 53.8%, and QQ = 24.2%) compared to European descendant type 2 DM patients (KK = 62.7%, KQ = 33.3%, and QQ = 4.1%) and BD (KK = 61.0%, KQ = 35.6%, and QQ = 3.4%). However, there was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ENPP1 polymorphism; Ethnicity; Type 2 diabetes mellitus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300009

The conservative physiology of the immune system
Provedor de dados: 56
Autores: Vaz,N.M.; Faria,A.M.C. de; Verdolin,B.A.; Silva Neto,A.F.; Menezes,J.S.; Carvalho,C.R.
Current immunological opinion disdains the necessity to define global interconnections between lymphocytes and regards natural autoantibodies and autoreactive T cells as intrinsically pathogenic. Immunological theories address the recognition of foreignness by independent clones of lymphocytes, not the relations among lymphocytes or between lymphocytes and the organism. However, although extremely variable in cellular/molecular composition, the immune system preserves as invariant a set of essential relations among its components and constantly enacts contacts with the organism of which it is a component. These invariant relations are reflected, for example, in the life-long stability of profiles of reactivity of immunoglobulins formed by normal organisms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunobiology; Immune system; Closure; Network; Autopoiesis; Panama blot.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100003

Baicalin ameliorates chronic mild stress-induced depression-like behaviors in mice and attenuates inflammatory cytokines and oxidative stress
Provedor de dados: 56
Autores: Zhong,Juying; Li,Gonghua; Xu,Hong; Wang,Yan; Shi,Mingming.
The natural flavonoid glycoside baicalin (BA) produces a variety of pharmaceutical effects, particularly for psychiatric/neurological disorders. This study evaluated the behavioral and neuroprotective effects of BA in mice subjected to chronic unpredictable mild stress, a model of depression. BA (25 and 50 mg/kg) significantly increased sucrose consumption and reduced immobility times in the tail suspension and forced swim tests, demonstrating that BA alleviated depression-like behaviors. Moreover, BA reduced the levels of inflammatory cytokines, such as interleukin 1β, interleukin 6, and tumor necrosis factor α, in serum and in the hippocampus. BA also abrogated increases in NMDAR/NR2B and Ca2+/calmodulin-dependent protein kinase II, and the decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baicalin; Depression; Neuroinflammatory; Oxidative stress.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700602

Cell migration in the postnatal subventricular zone
Provedor de dados: 56
Autores: Menezes,J.R.L.; Marins,M.; Alves,J.A.J.; Fróes,M.M.; Hedin-Pereira,C.
New neurons are constantly added to the olfactory bulb of rodents from birth to adulthood. This accretion is not only dependent on sustained neurogenesis, but also on the migration of neuroblasts and immature neurons from the cortical and striatal subventricular zone (SVZ) to the olfactory bulb. Migration along this long tangential pathway, known as the rostral migratory stream (RMS), is in many ways opposite to the classical radial migration of immature neurons: it is faster, spans a longer distance, does not require radial glial guidance, and is not limited to postmitotic neurons. In recent years many molecules have been found to be expressed specifically in this pathway and to directly affect this migration. Soluble factors with inhibitory, attractive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Postnatal neurogenesis; Chain migration; Rostral migratory stream; Astrocytes; Gap junctions; Radial glia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001200002

Association of loss of heterozygosity with cytogenetic abnormalities in acute myeloid leukemia and myelodysplastic syndrome
Provedor de dados: 56
Autores: Pinheiro,R.F.; Serio,F.M.; Silva,M.R.R.; Briones,M.R.S.; Chauffaille,M.L.L.F.
Deletions on chromosomes 5 and 7 are frequently seen in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). It is assumed that these deletions indicate loss of tumor suppressor genes on these chromosomes and until these tumor suppressor genes are identified, the functional consequences of these deletions and the molecular basis of these myeloid disorders cannot be completely understood. We evaluated loss of heterozygosity (LOH) in 44 patients (18 MDS and 26 AML, diagnosed according to WHO classification criteria) at diagnosis, using a four-microsatellite marker panel: an intragenic marker on the 7th intron of gene IRF-1 of the 5q31.1 region and three markers located inside the 7q31.1 region and correlated the LOH with karyotype abnormalities....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myelodysplastic syndrome; Acute myeloid leukemia; Cytogenetic abnormalities; Loss of heterozygosity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700010

Erythrocyte glucose-6-phosphate dehydrogenase activity assay and affinity for its substrate under "physiological" conditions
Provedor de dados: 56
Autores: Leite,A.A.; Barretto,O.C. de O.
Glucose-6-phosphate dehydrogenase (G6PD) activity and the affinity for its substrate glucose-6-phosphate were investigated under conditions similar to the physiological environment in terms of ionic strength (I: 0.188), cation concentration, pH 7.34, and temperature (37oC). A 12.4, 10.4 and 21.4% decrease was observed in G6PD B, G6PD A+ and G6PD A- activities, respectively. A Km increase of 95.1, 94.4 and 95.4% was observed in G6PD B, G6PD A+ and G6PD A-, respectively, leading to a marked decrease in affinity. In conclusion, the observation of the reduced activity and affinity for its natural substrate reflects the actual pentose pathway rate. It also suggests a much lower NADPH generation, which is crucial mostly in G6PD-deficient individuals, whose NADPH...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glucose-6-phosphate dehydrogenase; Red blood cells; G6PD activity and affinity.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200004

Effect of an isotonic rehydration sports drink and exercise on urolithiasis in rats
Provedor de dados: 56
Autores: Abreu,N.P.; Bergamaschi,C.T.; di Marco,G.S.; Razvickas,C.V.; Schor,N.
The objective of the present study was to evaluate the role of physical exercise as well as the influence of hydration with an isotonic sports drink on renal function in male Wistar rats. Four groups were studied over a period of 42 days: 1) control (N = 9); 2) physical exercise (Exe, N = 7); 3) isotonic drink (Drink, N = 8); 4) physical exercise + isotonic drink (Exe + Drink, N = 8). Physical exercise consisted of running on a motor-driven treadmill for 1 h/day, at 20 m/min, 5 days a week. The isotonic sports drink was a commercial solution used by athletes for rehydration after physical activity, 2 ml administered by gavage twice a day. Urine cultures were performed in all animals. Twenty-four-hour urine samples were collected in metabolic cages at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal function; Isotonic rehydration sports drink; Physical activity; Renal stone; Nephrolithiasis; Tamm-Horsfall protein.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400011

Texture analysis of computed tomography images of acute ischemic stroke patients
Provedor de dados: 56
Autores: Oliveira,M.S.; Fernandes,P.T.; Avelar,W.M.; Santos,S.L.M.; Castellano,G.; Li,L.M.
Computed tomography (CT) images are routinely used to assess ischemic brain stroke in the acute phase. They can provide important clues about whether to treat the patient by thrombolysis with tissue plasminogen activator. However, in the acute phase, the lesions may be difficult to detect in the images using standard visual analysis. The objective of the present study was to determine if texture analysis techniques applied to CT images of stroke patients could differentiate between normal tissue and affected areas that usually go unperceived under visual analysis. We performed a pilot study in which texture analysis, based on the gray level co-occurrence matrix, was applied to the CT brain images of 5 patients and of 5 control subjects and the results were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Texture analysis; Computed tomography; Thrombolysis; Discriminant analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100012

Effects of rosiglitazone on serum paraoxonase activity and metabolic parameters in patients with type 2 diabetes mellitus
Provedor de dados: 56
Autores: Atamer,Y.; Atamer,A.; Can,A.S.; Hekimoğlu,A.; Ilhan,N.; Yenice,N.; Koçyiğit,Y.
Human serum paraoxonase contributes to the anti-atherogenic effect of high-density lipoprotein cholesterol (HDL-C) and has been shown to protect both low-density lipoprotein cholesterol (LDL-C) and HDL-C against lipid peroxidation. We investigated the effects of rosiglitazone on paraoxonase activity and metabolic parameters in patients with type 2 diabetes mellitus [50 patients (30 males, 20 females); mean±SD age: 58.7±9.2 years, body mass index: 28.2±4.1'kg/m2], in whom glucose control could not be achieved despite treatment with metformin, sulphonylurea, and/or insulin. The patients were given 4'mg/day rosiglitazone for 3 months in addition to their usual treatment. Serum paraoxonase activity, malondialdehyde, homocysteine, and lipid profile were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosiglitazone; Diabetes mellitus type 2; Paraoxonase; Lipoproteins; Homocysteine; Lipid peroxidation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600528

Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection
Provedor de dados: 56
Autores: Zanetti,C.C.S.; Mingrone,R.C.C.; Kisielius,J.J.; Ueda-Ito,M.; Pignatari,A.C.C.
Some clinical isolates of Pseudomonas aeruginosa stored in our culture collection did not grow or grew poorly and showed lysis on the culture plates when removed from the collection and inoculated on MacConkey agar. One hypothesis was that bacteriophages had infected and killed those clinical isolates. To check the best storage conditions to maintain viable P. aeruginosa for a longer time, clinical isolates were stored at various temperatures and were grown monthly. We investigated the presence of phage in 10 clinical isolates of P. aeruginosa stored in our culture collection. Four strains of P. aeruginosa were infected by phages that were characterized by electron microscopy and isolated to assess their ability to infect. The best condition to maintain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriophages; Pseudomonas aeruginosa.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800689

Myosin light chain kinase is necessary for post-shock mesenteric lymph drainage enhancement of vascular reactivity and calcium sensitivity in hemorrhagic-shocked rats
Provedor de dados: 56
Autores: Zhang,Y.P.; Niu,C.Y.; Zhao,Z.G.; Zhang,L.M.; Si,Y.H.
Vascular hyporeactivity is an important factor in irreversible shock, and post-shock mesenteric lymph (PSML) blockade improves vascular reactivity after hemorrhagic shock. This study explored the possible involvement of myosin light chain kinase (MLCK) in PSML-mediated vascular hyporeactivity and calcium desensitization. Rats were divided into sham (n=12), shock (n=18), and shock+drainage (n=18) groups. A hemorrhagic shock model (40±2 mmHg, 3 h) was established in the shock and shock+drainage groups. PSML drainage was performed from 1 to 3 h from start of hypotension in shock+drainage rats. Levels of phospho-MLCK (p-MLCK) were determined in superior mesenteric artery (SMA) tissue, and the vascular reactivity to norepinephrine (NE) and sensitivity to Ca2+...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemorrhagic shock; Mesenteric lymph; Drainage; Myosin light chain kinase (MLCK); Vascular reactivity; Calcium sensitivity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700574

Practices and ethical concerns regarding preimplantation diagnosis. Who regulates preimplantation genetic diagnosis in Brazil?
Provedor de dados: 56
Autores: Damian,B.B.; Bonetti,T.C.S.; Horovitz,D.D.G.
Preimplantation genetic diagnosis (PGD) was originally developed to diagnose embryo-related genetic abnormalities for couples who present a high risk of a specific inherited disorder. Because this technology involves embryo selection, the medical, bioethical, and legal implications of the technique have been debated, particularly when it is used to select features that are not related to serious diseases. Although several initiatives have attempted to achieve regulatory harmonization, the diversity of healthcare services available and the presence of cultural differences have hampered attempts to achieve this goal. Thus, in different countries, the provision of PGD and regulatory frameworks reflect the perceptions of scientific groups, legislators, and...
Palavras-chave: Preimplantation genetic diagnosis; Public policy; Regulation; Ethics; Brazil.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100025

Epoetin alfa resistance in hemodialysis patients with chronic kidney disease: a longitudinal study
Provedor de dados: 56
Autores: Santos,E.J.F.; Hortegal,E.V.; Serra,H.O.; Lages,J.S.; Salgado-Filho,N.; dos Santos,A.M.
Anemia is an inevitable complication of hemodialysis, and the primary cause is erythropoietin deficiency. After diagnosis, treatment begins with an erythropoiesis-stimulating agent (ESA). However, some patients remain anemic even after receiving this medication. This study aimed to investigate the factors associated with resistance to recombinant human erythropoietin therapy with epoetin alfa (αEPO). We performed a prospective, longitudinal study of hemodialysis patients receiving treatment with αEPO at our reference hospital from July 2015 to June 2016. Clinical data was collected, and the response to αEPO treatment was evaluated using the erythropoietin resistance index (ERI). The ERI was defined as the weekly weight-adjusted αEPO dose (U/kg per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epoetin alfa; Drug resistance; Renal insufficiency; Chronic disease; Renal dialysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700603

Functional analysis of newly discovered growth control genes: experimental approaches
Provedor de dados: 56
Autores: Flatschart,R.B.; Sogayar,M.C.
A large number of DNA sequences corresponding to human and animal transcripts have been filed in data banks, as cDNAs or ESTs (expression sequence tags). However, the actual function of their corresponding gene products is still largely unknown. Several of these genes may play a role in regulation of important biological processes such as cell division, differentiation, malignant transformation and oncogenesis. Elucidation of gene function is based on 2 main approaches, namely, overexpression and expression interference, which respectively mimick or suppress a given phenotype. The currently available tools and experimental approaches to gene functional analysis and the most recent advances in mass cDNA screening by functional analysis are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene function; Transgene overexpression; Expression interference; Antisense; Ribozymes; Transgenic cells and animals; Genetic knock-out.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700011

Immunoexpression of cyclooxygenase-1 and -2 in ulcerative colitis
Provedor de dados: 56
Autores: Paiotti,A.P.R.; Artigiani Neto,R.; Forones,N.M.; Oshima,C.T.F.; Miszputen,S.J.; Franco,M.
Ulcerative colitis (UC) is a disease of the colon and rectum characterized by a nonspecific chronic inflammation mediated by the concerted response of cellular and humoral events. Prostaglandins are synthesized by cyclooxygenase (COX)-1 and -2 and exhibit both pro- and anti-inflammatory activity. To evaluate COX-1 and COX-2 immunoexpression in 42 cases of UC and to correlate it with clinicopathological parameters, COX-1 and COX-2 expression was investigated by the immunohistochemistry method. Only patients with all pertinent clinical and evolutive data as well as with adequate biopsy material were included in the study. Fifteen samples of colorectal adenocarcinoma and 14 of large bowel with no histological changes were used for positive and negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ulcerative colitis; Cyclooxygenases; Prostaglandins; Immunohistochemistry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700004

Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
Provedor de dados: 56
Autores: Passos,E.P.; Brugnara,L.; Facin,A.C.; Riffel,A.; Lima,G.R.; Freitas,V.; Brandelli,A.
The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrosome reaction; Endometriosis; Infertility; Peritoneal fluid; Sperm.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100009

The increased but non-predominant expression of Th17- and Th1-specific cytokines in Hashimoto’s thyroiditis but not in Graves’ disease
Provedor de dados: 56
Autores: Qin,Qiu; Liu,Ping; Liu,Lin; Wang,Rong; Yan,Ni; Yang,Jing; Wang,Xuan; Pandey,Madhu; Zhang,Jin-an.
Hashimoto’s thyroiditis (HT) is considered to be mediated mainly by Th1 cells, but it is not known whether Graves’ disease (GD) is associated with Th1 or Th2 predominance. Th17 cells, a novel subset of Th cells, play a crucial role in the pathogenesis of various autoimmune disorders. In the present study, the expression of IL-17A and IFN-γ was investigated in patients with HT or GD. mRNA expression of IL-17A and IFN-γ in peripheral blood mononuclear cells (PBMC) from 43 patients with autoimmune thyroid disease (AITD) and in thyroid tissues from 40 AITD patients were measured by real-time qRT-PCR. The protein expression of IL-17A and IL-23p19 was examined by immunohistochemistry in thyroid tissues from 28 AITD patients. The mRNA levels of IL-17A and IFN-γ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Graves’ disease; Hashimoto’s thyroiditis; Interleukin-17; Interferon-γ.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200013

Analysis of five streptokinase formulations using the euglobulin lysis test and the plasminogen activation assay
Provedor de dados: 56
Autores: Couto,L.T.; Donato,J.L.; Nucci,G. de.
Streptokinase, a 47-kDa protein isolated and secreted by most group A, C and G ß-hemolytic streptococci, interacts with and activates human protein plasminogen to form an active complex capable of converting other plasminogen molecules to plasmin. Our objective was to compare five streptokinase formulations commercially available in Brazil in terms of their activity in the in vitro tests of euglobulin clot formation and of the hydrolysis of the plasmin-specific substrate S-2251™. Euglobulin lysis time was determined using a 96-well microtiter plate. Initially, human thrombin (10 IU/ml) and streptokinase were placed in individual wells, clot formation was initiated by the addition of plasma euglobulin, and turbidity was measured at 340 nm every 30 s. In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptokinase; Plasminogen; Plasmin; Substrate S-2251™.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200015

An ontogenic study of the behavioral effects of chronic intermittent exposure to ayahuasca in mice
Provedor de dados: 56
Autores: Correa-Netto,N.F.; Masukawa,M.Y.; Nishide,F.; Galfano,G.S.; Tamura,F.; Shimizo,M.K.; Marcato,M.P.; Santos Junior,J.G.; Linardi,A.
Ayahuasca is a beverage obtained from decoctions of the Banisteriopsis caapi plus Psychotria viridis. In religious contexts, ayahuasca is used by different age groups. However, little is known of the effects of ayahuasca during ontogenic development, particularly with regard to the functional characteristics of the central nervous system. Animal models are useful for studying the ontogenic effects of ayahuasca because they allow exclusion of the behavioral influence associated with the ritualistic use. We investigated the effects of exposure to ayahuasca (1.5 mL/kg, orally, twice a week) on memory and anxiety in C57BL/6 mice, with the post-natal day (PND) being used as the ontogenic criterion for classification: childhood (PND21 to PND35), adolescence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adolescent Anxiety Banisteriopsis N; N-Dimethyltryptamine Infant Memory.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700603

Production of a cloned calf from a fetal fibroblast cell line
Provedor de dados: 56
Autores: Mello,M.R.B.; Caetano,H.V.A.; Marques,M.G.; Padilha,M.S.; Garcia,J.F.; Milazzotto,M.P.; Assumpção,M.E.O.A.; Lima,A.S.; Nicácio,A.C.; Mendes,C.M.; Oliveira,V.P.; Visintin,J.A.
The present study examined the in vitro and in vivo development of bovine nuclear-transferred embryos. A bovine fetal fibroblast culture was established and used as nucleus donor. Slaughterhouse oocytes were matured in vitro for 18 h before enucleation. Enucleated oocytes were fused with fetal fibroblasts with an electric stimulus and treated with cytochalasin D and cycloheximide for 1 h followed by cycloheximide alone for 4 h. Reconstructed embryos were cultured for 7-9 days and those which developed to blastocysts were transferred to recipient cows. Of 191 enucleated oocytes, 83 (43.5%) were successfully fused and 24 (28.9%) developed to blastocysts. Eighteen freshly cloned blastocysts were transferred to 14 recipients, 5 (27.8%) of which were pregnant...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cloning; Nuclear transfer; Bovine; Fetal fibroblasts; Embryo reconstruction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100006

Antinociceptive effect of novel pyrazolines in mice
Provedor de dados: 56
Autores: Tabarelli,Z.; Rubin,M.A.; Berlese,D.B.; Sauzem,P.D.; Missio,T.P.; Teixeira,M.V.; Sinhorin,A.P.; Martins,M.A.P.; Zanatta,N.; Bonacorso,H.G.; Mello,C.F.
The antinociceptive effect of six novel synthetic pyrazolines (3-ethoxymethyl-5-ethoxycarbonyl-1H-pyrazole (Pz 1) and its corresponding 1-substituted methyl (Pz 2) and phenyl (Pz 3) analogues, and 3-(1-ethoxyethyl)-5-ethoxycarbonyl-1H-pyrazole (Pz 4) and its corresponding 1-substituted methyl (Pz 5) and phenyl (Pz 6) analogues) was evaluated by the tail immersion test in adult male albino mice. The animals (N = 11-12 in each group) received vehicle (5% Tween 80, 10 ml/kg, sc) or 1.5 mmol/kg of each of the pyrazolines (Pz 1-Pz 6), sc. Fifteen, thirty and sixty minutes after drug administration, the mice were subjected to the tail immersion test. Thirty minutes after drug administration Pz 2 and Pz 3 increased tail withdrawal latency (vehicle = 3.4 ± 0.2; Pz...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrazole-derived compounds; Antinociception; Tail immersion test; Rotarod test; Open-field test; Immobility.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000013

Electrophysiological measurements of spectral sensitivities: a review
Provedor de dados: 56
Spectral sensitivities of visual systems are specified as the reciprocals of the intensities of light (quantum fluxes) needed at each wavelength to elicit the same criterion amplitude of responses. This review primarily considers the methods that have been developed for electrophysiological determinations of criterion amplitudes of slow-wave responses from single retinal cells. Traditional flash methods can require tedious dark adaptations and may yield erroneous spectral sensitivity curves which are not seen in such modifications as ramp methods. Linear response methods involve interferometry, while constant response methods involve manual or automatic adjustments of continuous illumination to keep response amplitudes constant during spectral scans. In DC...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Spectral sensitivity; Color vision; Electrophysiology; Experimental automation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200003

Application of endoscopy to treat mandibular keratocystic odontogenic tumors
Provedor de dados: 56
Autores: Gao,Z.; Ni,Q.W.; Gao,W.; Liu,Y.P.; Zhang,Q.
The aim of this study was to evaluate the feasibility of endoscopy to remove keratocystic odontogenic tumors (KCOTs) with virtual 3D mandibular images. Fifteen patients (mean age, 40.27±14.58 years) who underwent endoscopic mandibular KCOT enucleation between May 2009 and October 2009 were included. Virtual 3D mandibular reconstructions derived from computed tomography (CT) imaging were generated for all patients. Recurrence and pathological fracture were evaluated as the primary outcome variables at 1 and 12 months after operation. Secondary infection and inferior alveolar nerve injury were evaluated as the secondary outcome variables at 1 and 6 months after operation. None of the 15 patients exhibited signs of recurrence or pathological fracture after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mandibular KCOT; Endoscopic assisted surgery; 3D reconstruction; Minimally invasive surgical procedures; Treatment feasibility.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800605

Generalization of temporal order detection skill learning: two experimental studies of children with dyslexia
Provedor de dados: 56
Autores: Murphy,C.F.B.; Schochat,E.
The objective of this study was to investigate the phenomenon of learning generalization of a specific skill of auditory temporal processing (temporal order detection) in children with dyslexia. The frequency order discrimination task was applied to children with dyslexia and its effect after training was analyzed in the same trained task and in a different task (duration order discrimination) involving the temporal order discrimination too. During study 1, one group of subjects with dyslexia (N = 12; mean age = 10.9 ± 1.4 years) was trained and compared to a group of untrained dyslexic children (N = 28; mean age = 10.4 ± 2.1 years). In study 2, the performance of a trained dyslexic group (N = 18; mean age = 10.1 ± 2.1 years) was compared at three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temporal order detection; Learning; Dyslexia; Children.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400007

Percutaneous 17ß-estradiol replacement therapy in hypertensive postmenopausal women
Provedor de dados: 56
Autores: Osório-Wender,M.C.; Vitola,D.; Spritzer,P.M.
The present study evaluated the short-term effects of percutaneous 17ß-estradiol on blood pressure, metabolic profile and hormonal levels in postmenopausal women with systemic arterial hypertension. After a wash-out period of 15 days, 10 hypertensive patients were treated with guanabenz acetate to control blood pressure, followed by 17ß-estradiol in the form of hydroalcoholic gel administered for 21 of 28 days of each cycle, for 3 cycles. Patients were evaluated before, during and 2 months after estrogen administration. Systolic and diastolic blood pressure or heart rate did not present any significant change in any patient when compared to those periods with the antihypertensive drug only (pretreatment period and 60 days after estrogen therapy was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Menopause; Hormone replacement therapy; Hypertension; 17ß-estradiol; LH; FSH.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900001

Adeno-associated virus for cystic fibrosis gene therapy
Provedor de dados: 56
Autores: Martini,S.V.; Rocco,P.R.M.; Morales,M.M.
Gene therapy is an alternative treatment for genetic lung disease, especially monogenic disorders such as cystic fibrosis. Cystic fibrosis is a severe autosomal recessive disease affecting one in 2500 live births in the white population, caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR). The disease is classically characterized by pancreatic enzyme insufficiency, an increased concentration of chloride in sweat, and varying severity of chronic obstructive lung disease. Currently, the greatest challenge for gene therapy is finding an ideal vector to deliver the transgene (CFTR) to the affected organ (lung). Adeno-associated virus is the most promising viral vector system for the treatment of respiratory disease because it...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene therapy; Adeno-associated virus; Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator; Vectors.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100004

Protein defects in neuromuscular diseases
Provedor de dados: 56
Autores: Vainzof,M.; Zatz,M.
Muscular dystrophies are a heterogeneous group of genetically determined progressive disorders of the muscle with a primary or predominant involvement of the pelvic or shoulder girdle musculature. The clinical course is highly variable, ranging from severe congenital forms with rapid progression to milder forms with later onset and a slower course. In recent years, several proteins from the sarcolemmal muscle membrane (dystrophin, sarcoglycans, dysferlin, caveolin-3), from the extracellular matrix (alpha2-laminin, collagen VI), from the sarcomere (telethonin, myotilin, titin, nebulin), from the muscle cytosol (calpain 3, TRIM32), from the nucleus (emerin, lamin A/C, survival motor neuron protein), and from the glycosylation pathway (fukutin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscular dystrophies; Muscle proteins; Dystrophin; Sarcoglycans.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500001

Whole-body vibration decreases the proliferativeb response of TCD4+ cells in elderly individuals with knee osteoarthritis
Provedor de dados: 56
Autores: Tossige-Gomes,R.; Avelar,N.C.P.; Simão,A.P.; Neves,C.D.C.; Brito-Melo,G.E.A.; Coimbra,C.C.; Rocha-Vieira,E.; Lacerda,A.C.R.
The aim of this study was to investigate the effect of adding whole-body vibration (WBV; frequency = 35 to 40 Hz; amplitude = 4 mm) to squat training on the T-cell proliferative response of elderly patients with osteoarthritis (OA) of the knee. This study was a randomized controlled trial in which the selected variables were assessed before and after 12 weeks of training. Twenty-six subjects (72 ± 5 years of age) were divided into three groups: 1) squat training with WBV (WBV, N = 8); 2) squat training without WBV (N = 10), and 3) a control group (N = 8). Women who were ≥60 years of age and had been diagnosed with OA in at least one knee were eligible. The intervention consisted of 12 uninterrupted weeks of squatting exercise training performed 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vibratory platform; Lymphocyte; Proliferation; Elderly; Osteoarthritis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200022

Erythrocytes may contain a ouabain-insensitive K+-ATPase which plays a role in internal K+ balance
Provedor de dados: 56
Autores: Seguro,L.F.B.C.; Seguro,D.L.B.C.; Helou,C.M.B.
Erythrocytes are useful in evaluating K+ transport pathways involved in internal K+ balance. Several forms of H+,K+-ATPase have been described in nephron segments active in K+ transport. Furthermore, the activity of a ouabain-insensitive isoform of H+,K+-ATPase expressed in collecting duct cells may be modulated by acid-base status. Various assays were performed to determine if a ouabain-insensitive K+-ATPase is present in rat erythrocytes and, if so, whether it plays a role in internal K+ balance. Kinetic studies demonstrated that maximal stimulation of enzyme activity was achieved with 2.5 mM K+ at pH 7.4. Subsequent experiments were performed on erythrocyte membranes collected from animals submitted to varying degrees of K+ homeostasis: control rats,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Erythrocyte K+-ATPase Potassium Acid-base status H+; K+-ATPase Ouabain.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200020

Psychiatric disorders and cardiac anxiety in exercising and sedentary coronary artery disease patients: a case-control study
Provedor de dados: 56
Autores: Sardinha,A.; Araújo,C.G.S.; Nardi,A.E.
Regular physical exercise has been shown to favorably influence mood and anxiety; however, there are few studies regarding psychiatric aspects of physically active patients with coronary artery disease (CAD). The objective of the present study was to compare the prevalence of psychiatric disorders and cardiac anxiety in sedentary and exercising CAD patients. A total sample of 119 CAD patients (74 men) were enrolled in a case-control study. The subjects were interviewed to identify psychiatric disorders and responded to the Cardiac Anxiety Questionnaire. In the exercise group (N = 60), there was a lower prevalence (45 vs 81%; P &lt; 0.001) of at least one psychiatric diagnosis, as well as multiple comorbidities, when compared to the sedentary group (N =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; Mental health; Depression; Cardiovascular disease; Cardiac rehabilitation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200031

Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine
Provedor de dados: 56
Autores: Suke,S.G.; Ahmed,R.S.; Pathak,R.; Tripathi,A.K.; Banerjee,B.D.
The effect of N-acetylcysteine, a thiolic antioxidant, on attenuation of phosphamidon-induced oxidative stress and immune dysfunction was evaluated in adult male Wistar rats weighing 200-250 g. Rats were divided into four groups, 8 animals/group, and treated with phosphamidon, N-acetylcysteine or the combination of both for 28 days. Oral administration of phosphamidon (1.74 mg/kg), an organophosphate insecticide, increased serum malondialdehyde (3.83 ± 0.18 vs 2.91 ± 0.24 nmol/mL; P &lt; 0.05) and decreased erythrocyte superoxide dismutase (567.8 ± 24.36 vs 749.16 ± 102.61 U/gHb; P &lt; 0.05), catalase activity (1.86 ± 0.18 vs 2.43 ± 0.08 U/gHb; P &lt; 0.05) and whole blood glutathione levels (1.25 ± 0.21 vs 2.28 ± 0.08 mg/gHb; P &lt; 0.05) showing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Antioxidant; Organophosphate; Oxidative stress; Immunotoxicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900004

Nitro-fatty acids: novel anti-inflammatory lipid mediators
Provedor de dados: 56
Autores: Rubbo,H.
Nitro-fatty acids are formed and detected in human plasma, cell membranes, and tissue, modulating metabolic as well as inflammatory signaling pathways. Here we discuss the mechanisms of nitro-fatty acid formation as well as their key chemical and biochemical properties. The electrophilic properties of nitro-fatty acids to activate anti-inflammatory signaling pathways are discussed in detail. A critical issue is the influence of nitroarachidonic acid on prostaglandin endoperoxide H synthases, redirecting arachidonic acid metabolism and signaling. We also analyze in vivo data supporting nitro-fatty acids as promising pharmacological tools to prevent inflammatory diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitro-fatty acids; Antioxidants; Nitric oxide; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900728

Proconvulsant effects of high doses of venlafaxine in pentylenetetrazole-convulsive rats
Provedor de dados: 56
Autores: Santos Junior,J.G.; Do Monte,F.H.M.; Russi,M.; Agustine,P.E.; Lanziotti,V.M.N.B.
Venlafaxine, an atypical antidepressant drug, has been used to treat several neurological disorders, presenting excellent efficacy and tolerability. Clinical seizures after venlafaxine treatment have occasionally been reported when the drug was used at very high doses or in combination with other medications. The aim of the present study was to investigate the convulsant effects of venlafaxine in rats under controlled laboratory conditions. Adult male Wistar rats (8 per group) receiving venlafaxine or saline at the doses of 25-150 mg/kg were subjected 30 min later to injections of pentylenetetrazole at the dose of 60 mg/kg. The animals receiving 75, 100 and 150 mg/kg venlafaxine presented increased severity of convulsion when compared to controls (P =...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antidepressant; Venlafaxine; Convulsion; Epilepsy; Pentylenetetrazole.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400010

Evaluation by blue native polyacrylamide electrophoresis colorimetric staining of the effects of physical exercise on the activities of mitochondrial complexes in rat muscle
Provedor de dados: 56
Autores: Molnar,A.M.; Alves,A.A.; Pereira-da-Silva,L.; Macedo,D.V.; Dabbeni-Sala,F.
Blue native polyacrylamide electrophoresis (BN-PAGE) is a technique developed for the analysis of membrane complexes. Combined with histochemical staining, it permits the analysis and quantification of the activities of mitochondrial oxidative phosphorylation enzymes using whole muscle homogenates, without the need to isolate muscle mitochondria. Mitochondrial complex activities were measured by emerging gels in a solution containing all specific substrates for NADH dehydrogenase and cytochrome c oxidase enzymes (complexes I and IV, respectively) and the colored bands obtained were measured by optique densitometry. The objective of the present study was the application of BN-PAGE colorimetric staining for enzymatic characterization of mitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BN-PAGE; Colorimetric staining; Exhaustive physical exercise; Mitochondrial complexes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700001

Anti-placental growth factor antibody ameliorates hyperoxia-mediated impairment of lung development in neonatal rats
Provedor de dados: 56
Autores: Zhang,Zhiqun; Zhong,Ying; Li,Xiaoxia; Huang,Xianmei; Du,Lizhong.
This study investigates the effect of the overexpression of the placental growth factor (PGF) and hyperoxia on lung development and determines whether anti-PGF antibody ameliorates hyperoxia-mediated impairment of lung development in newborn rats. After exposure to normoxic conditions for seven days, newborn rats subjected to normoxia were intraperitoneally or intratracheally injected with physiological saline, adenovirus-negative control (Ad-NC), or adenovirus-PGF (Ad-PGF) to create the Normoxia, Normoxia+Ad-NC, and Normoxia+Ad-PGF groups, respectively. Newborn rats subjected to hyperoxia were intraperitoneally injected with physiological saline or anti-PGF antibodies to create the Hyperoxia and Hyperoxia+anti-PGF groups, respectively. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bronchopulmonary dysplasia; Anti-PGF antibody; Newborn rats; Hyperoxia; Lung tissue injury.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200604

Lipid peroxidation, detoxification capacity, and genome damage in mice after transplacental exposure to pharmaceutical drugs
Provedor de dados: 56
Autores: Markovic,D.; Katic,J.; Stojkovic,R.; Borovic,S.; Zarkovic,N.; Fucic,A.
Data on genome damage, lipid peroxidation, and levels of glutathione peroxidase (GPX) in newborns after transplacental exposure to xenobiotics are rare and insufficient for risk assessment. The aim of the current study was to analyze, in an animal model, transplacental genotoxicity, lipid peroxidation, and detoxification disturbances caused by the following drugs commonly prescribed to pregnant women: paracetamol, fluconazole, 5-nitrofurantoin, and sodium valproate. Genome damage in dams and their newborn pups transplacentally exposed to these drugs was investigated using the in vivo micronucleus (MN) assay. The drugs were administered to dams intraperitoneally in three consecutive daily doses between days 12 and 14 of pregnancy. The results were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione peroxidase; Malondialdehyde; Genome damage; Transplacental exposure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201014

Rimonabant improves metabolic parameters partially attributed to restoration of high voltage-activated Ca2+ channels in skeletal muscle in HFD-fed mice
Provedor de dados: 56
Autores: Chen,B.; Hu,N.
Cannabinoid type 1 receptor (CB1R) inhibition tends to be one of the promising strategies for the treatment of obesity and other related metabolic disorders. Although CB1R inhibition may cause adverse psychiatric effects including depression and anxiety, the investigation of the role of peripheral CB1R on weight loss and related metabolic parameters are urgently needed. We first explored the effect of rimonabant, a selective CB1R antagonist/inverse agonist, on some metabolic parameters in high fat-diet (HFD)-induced obesity in mice. Then, real-time PCR and electrophysiology were used to explore the contribution of high voltage-activated Ca2+ channels (HVACCs), especially Cav1.1, on rimonabant's effect in skeletal muscle (SM) in HFD-induced obesity....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cannabinoid receptor type 1; Obesity; Rimonabant; Skeletal Muscle; HVA calcium channel.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600602

Delay in gallbladder emptying during the perimenopausal period
Provedor de dados: 56
Autores: Petroianu,A.; Oliveira,L.C.; Vieira,M.I.
A pilot study has ascribed an important role in gallbladder motility and emptying to the perimenopausal period. To assess the effect of this period on gallbladder emptying and cholelithogenesis, 25 women in the perimenopausal period without gallbladder disease were submitted to cholangiography and two ultrasound exams. The time for gallbladder emptying and the presence of cholelithiasis were assessed. All patients presented a delay in gallbladder emptying with no relationship to the pre- or postmenopausal period. This finding was not related to lithogenesis. Gallbladder emptying time is longer during the perimenopausal period.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gallbladder emptying; Menopause; Cholelithiasis; Ultrasound; Cholangiography.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900007

Purification of bovine pancreatic glucagon as a by-product of insulin production
Provedor de dados: 56
Autores: Andrade,A.S.R.; Vilela,L.; Tunes,H.
A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation. The isoelectric precipitate containing glucagon was then purified by ion-exchange chromatography on Q-Sepharose FF, gel filtration on Sephadex G-25 and ion-exchange chromatography on S-Sepharose FF. A pilot scale test was performed with a recovery of 87.6% and a purification factor of 8.78 for the first chromatographic step, a recovery of 75.1% and a purification factor of 3.90 for the second, and a recovery of 76.2% and a purification factor of 2.36 for the last one. The overall yield was 50%, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucagon; Protein purification; Gel filtration; Ion-exchange chromatography; Insulin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200006

Liver cancer stem cells are selectively enriched by low-dose cisplatin
Provedor de dados: 56
Autores: Zhang,H.; Chang,W.J.; Li,X.Y.; Zhang,N.; Kong,J.J.; Wang,Y.F.
Accumulating evidence has indicated the importance of cancer stem cells in carcinogenesis. The goal of the present study was to determine the effect of low-dose cisplatin on enriched liver cancer stem cells (LCSCs). Human hepatoblastoma HepG2 cells were treated with concentrations of cisplatin ranging from 1 to 5 μg/mL. Cell survival and proliferation were evaluated using a tetrazolium dye (MTT) assay. LCSCs were identified using specific markers, namely aldehyde dehydrogenase-1 (ALDH1) and CD133. The percentage of ALDH1+ or CD133+ cells was examined by flow cytometric analysis. The expression of ALDH1 and/or CD133 in HepG2 cells was determined by immunocytochemical analysis. Low-dose cisplatin treatment significantly decreased cell survival in HepG2 cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Liver cancer stem cell; HepG2; ALDH1; CD133.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600478

Abnormal nocturnal blood pressure fall in normotensive adolescents with insulin-dependent diabetes is ameliorated following glycemic improvement
Provedor de dados: 56
Autores: Ferreira,S.R.G.; Cesarini,P.R.; Vivolo,M.A.; Zanella,M.T.
Lack of the physiological nocturnal fall in blood pressure (BP) has been found in diabetics and it seems to be related to the presence of diabetic complications. The present study examined the changes in the nocturnal BP pattern of 8 normotensive insulin-dependent diabetic adolescents without nephropathy following improvement in glycemic control induced by an 8-day program of adequate diet and exercise. The same number of age- and sex-matched control subjects were studied. During the first and eighth nights of the program, BP was obtained by ambulatory BP monitoring. After a 10-min rest, 3 BP and heart rate (HR) recordings were taken and the mean values were considered to represent their awake values. The monitor was programmed to cuff insufflation every...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin/dependent diabetes mellitus; Blood pressure; Glycemic control.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400008

alpha-Globin genes: thalassemic and structural alterations in a Brazilian population
Provedor de dados: 56
Autores: Wenning,M.R.S.C.; Kimura,E.M.; Costa,F.F.; Saad,S.T.O.; Gervásio,S.; de Jorge,S.B.; Borges,E.; Silva,N.M.; Sonati,M.F.
Seven unrelated patients with hemoglobin (Hb) H disease and 27 individuals with alpha-chain structural alterations were studied to identify the alpha-globin gene mutations present in the population of Southeast Brazil. The -alpha3.7, --MED and -(alpha)20.5 deletions were investigated by PCR, whereas non-deletional alpha-thalassemia (alphaHphalpha, alphaNcoIalpha, &lt;FONT FACE="Symbol"&gt;aa&lt;/FONT&gt;NcoI, alphaIcalpha and alphaTSaudialpha) was screened with restriction enzymes and by nested PCR. Structural alterations were identified by direct DNA sequencing. Of the seven patients with Hb H disease, all of Italian descent, two had the -(alpha)20.5/-alpha3.7 genotype, one had the --MED/-alpha3.7 genotype, one had the --MED/alphaHphalpha genotype and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-globin genes; Alpha-globin structural variants; Alpha-thalassemia; Hemoglobin H; Hb H disease; Hemoglobin variants; Hemoglobinopathies.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900008

Evaluation of renal function in sickle cell disease patients in Brazil
Provedor de dados: 56
Autores: Silva Junior,G.B.; Libório,A.B.; Vieira,A.P.F.; Bem,A.X. Couto; Lopes Filho,A.S.; Figueiredo Filho,A.C.; Guedes,A.L.M.O.; Souza,J.H.; Costa,C.M.B.E.; Costa,R.; Daher,E.F.
The objective of this study was to investigate renal function in a cohort of 98 patients with sickle cell disease (SCD) followed up at a tertiary hospital in Brazil. Clinical and laboratory characteristics at the time of the most recent medical examination were analyzed. Renal function was evaluated by the estimation of glomerular filtration rate (GFR) by the criteria of the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI). We compared patients with normal GFR to patients with decreased GFR (&lt;60 mL·min-1·(1.73 m²)-1) and hyperfiltration (&amp;gt;120 mL·min-1·(1.73 m²)-1). Comparison between patients according to the use of hydroxyurea and comparison of clinical and laboratory parameters according to GFR were also carried out. Average patient...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sickle cell disease; Kidney disease; Glomerular filtration rate; Creatinine; Chronic kidney disease; Hydroxyurea.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700013

A model of hemorrhagic cystitis induced with acrolein in mice
Provedor de dados: 56
Autores: Batista,C.K.L.P.; Brito,G.A.C.; Souza,M.L.P.; Leitão,B.T.A.; Cunha,F.Q.; Ribeiro,R.A.
Acrolein is a urinary metabolite of cyclophosphamide and ifosfamide, which has been reported to be the causative agent of hemorrhagic cystitis induced by these compounds. A direct cytotoxic effect of acrolein, however, has not yet been demonstrated. In the present study, the effects of intravesical injection of acrolein and mesna, the classical acrolein chemical inhibitor, were evaluated. Male Swiss mice weighing 25 to 35 g (N = 6 per group) received saline or acrolein (25, 75, 225 µg) intravesically 3, 6, 12, and 24 h before sacrifice for evaluation of bladder wet weight, macroscopic and histopathological changes by Gray's criteria, and 3 and 24 h for assessment of increase in vascular permeability. In other animals, mesna was administered intravesically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrolein; Bladder; Hemorrhagic cystitis; Mesna.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100011

Evaluation of volume responsiveness by pulse pressure variability and inferior vena cava dispensability index at different tidal volumes by mechanical ventilation
Provedor de dados: 56
Autores: He,Fujuan; Li,Xiaoqiang; Thapa,Suman; Li,Chi; Luo,Jiawei; Dai,Wenyan; Liu,Jin.
This study investigated the effects of tidal volume (TV) on the diagnostic value of pulse pressure variation (PPV) and the inferior vena cava dispensability index (IVC-DI) for volume responsiveness during mechanical ventilation. In patients undergoing elective surgery with mechanical ventilation, different TVs of 6, 9, and 12 mL/kg were given for two min. The left ventricular outflow tract velocity-time integral (VTI) was measured by transthoracic echocardiography. The IVC-DI was measured at sub-xyphoid transabdominal long axis. The PPV was measured via the radial artery and served as baseline. Index measurements were repeated after fluid challenge. VTI increased by more than 15% after fluid challenge, which was considered as volume responsive....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulse pressure variation; Inferior vena cava dispensability index; Volume responsiveness; Tidal volume; Mechanical ventilation; Fluid challenge.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900607

Specific fluorogenic substrates for neprilysin (neutral endopeptidase, EC 3.4.24.11) which are highly resistant to serine- and metalloproteases
Provedor de dados: 56
Autores: Medeiros,M.A.S.; França,M.S.F.; Boileau,G.; Juliano,L.; Carvalho,K.M.
Two intramolecularly quenched fluorogenic peptides containing o-aminobenzoyl (Abz) and ethylenediamine 2,4-dinitrophenyl (EDDnp) groups at amino- and carboxyl-terminal amino acid residues, Abz-&lt;!-- $MVD$:face("Times") --&gt;DArg-Arg-Leu-EDDnp (Abz-&lt;!-- $MVD$:face("Times") --&gt;DRRL-EDDnp) and Abz-&lt;!-- $MVD$:face("Times") --&gt;DArg-Arg-Phe-EDDnp (Abz-&lt;!-- $MVD$:face("Times") --&gt;DRRF-EDDnp), were selectively hydrolyzed by neutral endopeptidase (NEP, enkephalinase, neprilysin, EC 3.4.24.11) at the Arg-Leu and Arg-Phe bonds, respectively. The kinetic parameters for the NEP-catalyzed hydrolysis of Abz-&lt;!-- $MVD$:face("Times") --&gt;DRRL-EDDnp and Abz-&lt;!-- $MVD$:face("Times") --&gt;DRRF-EDDnp were Km = 2.8 µM, kcat = 5.3 min-1, kcat/Km = 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutral endopeptidase; Enkephalinase; Neprilysin; Fluorogenic substrates; Phosphoramidon.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000003

The expression of melanopsin and clock genes in Xenopus laevis melanophores and their modulation by melatonin
Provedor de dados: 56
Autores: Bluhm,A.P.C.; Obeid,N.N.; Castrucci,A.M.L.; Visconti,M.A.
Vertebrates have a central clock and also several peripheral clocks. Light responses might result from the integration of light signals by these clocks. The dermal melanophores of Xenopus laevis have a photoreceptor molecule denominated melanopsin (OPN4x). The mechanisms of the circadian clock involve positive and negative feedback. We hypothesize that these dermal melanophores also present peripheral clock characteristics. Using quantitative PCR, we analyzed the pattern of temporal expression of Opn4x and the clock genes Per1, Per2, Bmal1, and Clock in these cells, subjected to a 14-h light:10-h dark (14L:10D) regime or constant darkness (DD). Also, in view of the physiological role of melatonin in the dermal melanophores of X. laevis, we determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xenopus laevis; Dermal melanophores; Melatonin; Melanopsin; Per1; Per2; Bmal1; Clock.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800007

Argyrophil cell density in the oxyntic mucosa is higher in female than in male morbidly obese patients
Provedor de dados: 56
Autores: Maksud,F.A.N.; Kakehasi,A.M.; Barbosa,A.J.A.
Obesity is a multifactorial disorder often associated with many important diseases such as diabetes, hypertension and other metabolic syndrome conditions. Argyrophil cells represent almost the total population of endocrine cells of the human gastric mucosa and some reports have described changes of specific types of these cells in patients with obesity and metabolic syndrome. The present study was designed to evaluate the global population of argyrophil cells of the gastric mucosa of morbidly obese and dyspeptic non-obese patients. Gastric biopsies of antropyloric and oxyntic mucosa were obtained from 50 morbidly obese patients (BMI &gt;40) and 50 non-obese patients (17 dyspeptic overweight and 33 lean individuals) and processed for histology and Grimelius...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Argyrophil cells; Endocrine cells; Obesity; Gastric mucosa; Metabolic syndrome.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400339

Enhanced production and organic solvent stability of a protease fromBrevibacillus laterosporus strain PAP04
Provedor de dados: 56
Autores: Anbu,P.
A bacterial strain (PAP04) isolated from cattle farm soil was shown to produce an extracellular, solvent-stable protease. Sequence analysis using 16S rRNA showed that this strain was highly homologous (99%) to Brevibacillus laterosporus. Growth conditions that optimize protease production in this strain were determined as maltose (carbon source), skim milk (nitrogen source), pH 7.0, 40°C temperature, and 48 h incubation. Overall, conditions were optimized to yield a 5.91-fold higher production of protease compared to standard conditions. Furthermore, the stability of the enzyme in organic solvents was assessed by incubation for 2 weeks in solutions containing 50% concentration of various organic solvents. The enzyme retained activity in all tested solvents...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brevibacillus; Organic solvent-stable protease; Peptide synthesis; Solvent stability.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400604

Translation into Brazilian Portuguese, cultural adaptation and evaluation of the reliability of the Disabilities of the Arm, Shoulder and Hand Questionnaire
Provedor de dados: 56
Autores: Orfale,A.G.; Araújo,P.M.P.; Ferraz,M.B.; Natour,J.
The objective of the present study was to translate, adapt and validate a Brazilian Portuguese version of the Disabilities of the Arm, Shoulder and Hand (DASH) Questionnaire. The study was carried out in two steps. The first was to translate the DASH into Portuguese and to perform cultural adaptation and the second involved the determination of the reliability and validity of the DASH for the Brazilian population. For this purpose, 65 rheumatoid arthritis patients of either sex (according to the classification criteria of the American College of Rheumatology), ranging in age from 18 to 60 years and presenting no other diseases involving the upper limbs, were interviewed. The patients were selected consecutively at the rheumatology outpatient clinic of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disabilities of the Arm; Shoulder and Hand (DASH); Translation into Brazilian Portuguese; Disability; Questionnaire; Arthritis; Upper limb; Validation; Arm.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200018

Correction: Braz J Med Biol Res 2006; 39 (12): 1575-1580: HFE gene mutations in Brazilian thalassemic patients
Provedor de dados: 56
Autores: Oliveira,T.M.; Souza,F.P.; Jardim,A.C.G.; Cordeiro,J.A.; Pinho,J.R.R.; Sitnik,R.; Estevão,I.F.; Bonini-Domingos,C.R.; Rahal,P.
Tipo: Info:eu-repo/semantics/report
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100018

Minimal doses of hydroxyurea for sickle cell disease
Provedor de dados: 56
Autores: Lima,C.S.P.; Arruda,V.R.; Costa,F.F.; Saad,S.T.O.
The use of hydroxyurea (HU) can improve the clinical course of sickle cell disease. However, several features of HU treatment remain unclear, including the predictability of drug response and determination of adequate doses, considering positive responses and minimal side effects. In order to identify adequate doses of HU for treatment of sickle cell disease, 10 patients, 8 with sickle cell anemia and 2 with Sß thalassemia (8SS, 2Sß), were studied for a period of 6 to 19 months in an open label dose escalation trial (10 to 20 mg kg-1 day-1). Hemoglobin (Hb), fetal hemoglobin (Hb F) and mean corpuscular volume (MCV) values and reticulocyte, neutrophil and platelet counts were performed every two weeks during the increase of the HU dose and every 4 weeks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell disease; Hydroxyurea; Hemoglobinopathy.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800004

Anxiolytic effect of methylene blue microinjected into the dorsal periaqueductal gray matter
Provedor de dados: 56
Autores: de-Oliveira,R.W.; Guimarães,F.S.
The dorsal periaqueductal gray (DPAG) has been implicated in the behavioral and autonomic expression of defensive reactions. Several results suggest that, along with GABA, glutamate and serotonin, nitric oxide (NO) may play a role in defense reactions mediated by this region. To further investigate this possibility we microinjected methylene blue (MB; 10, 30 or 100 nmol/0.5 µl) into the DPAG of rats submitted to the elevated plus-maze test, an animal model of anxiety. MB has been used as an inhibitor of soluble guanylate cyclase (sGC) to demonstrate cGMP-mediated processes, and there is evidence that NO may exert its biological effects by binding to the heme part of guanylate cyclase, causing an increase in cGMP levels. The results showed that MB (30 nmol)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anxiety; Elevated plus-maze; Nitric oxide; Animal model.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200012

Immobilization and therapeutic passive stretching generate thickening and increase the expression of laminin and dystrophin in skeletal muscle
Provedor de dados: 56
Autores: Cação-Benedini,L.O.; Ribeiro,P.G.; Prado,C.M.; Chesca,D.L.; Mattiello-Sverzut,A.C.
Extracellular matrix and costamere proteins transmit the concentric, isometric, and eccentric forces produced by active muscle contraction. The expression of these proteins after application of passive tension stimuli to muscle remains unknown. This study investigated the expression of laminin and dystrophin in the soleus muscle of rats immobilized with the right ankle in plantar flexion for 10 days and subsequent remobilization, either by isolated free movement in a cage or associated with passive stretching for up to 10 days. The intensity of the macrophage response was also evaluated. One hundred and twenty-eight female Wistar rats were divided into 8 groups: free for 10 days; immobilized for 10 days; immobilized/free for 1, 3, or 10 days; or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Muscle stretching; Soleus muscle; Laminin; Dystrophin; Mechanotransduction.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600483

Electrocardiographic changes during low-dose, short-term therapy of cutaneous leishmaniasis with the pentavalent antimonial meglumine
Provedor de dados: 56
Autores: Ribeiro,A.L.P.; Drummond,J.B.; Volpini,A.C.; Andrade,A.C.; Passos,V.M.A.
The pentavalent antimonial (Sb5+) meglumine is the drug of choice for the treatment of cutaneous leishmaniasis (CL) in Brazil. Although the cardiotoxicity of high-dose, long-term Sb5+ therapy is well known, the use of low-dose, short-term meglumine has been considered to be safe and relatively free from significant cardiac effects. In order to investigate the cardiotoxicity of low-dose, short-term therapy with meglumine in cutaneous leishmaniasis, 62 CL patients treated with meglumine were studied. A standard ECG was obtained before and immediately after the first cycle of treatment (15 mg Sb5+ kg-1 day-1). The electrocardiographic interpretation was carried out blindly by two investigators using the Minnesota Code. There were no significant differences in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cutaneous leishmaniasis; Electrocardiography; QT interval.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300008

Inducible nitric oxide synthase and tumor necrosis factor-alpha in delayed gastric emptying and gastrointestinal transit induced by lipopolysaccharide in mice
Provedor de dados: 56
Autores: Inada,T.; Hamano,N.; Yamada,M.; Shirane,A.; Shingu,K.
Gastrointestinal motility disturbances during endotoxemia are probably caused by lipopolysaccharide (LPS)-induced factors: candidates include nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-1ß, and interleukin-6. Flow cytometry was used to determine the effects of LPS and these factors on gastric emptying (evaluated indirectly by determining percent gastric retention; %GR) and gastrointestinal transit (GIT) in male BALB/c mice (23-28 g). NO (300 µg/mouse, N = 8) and TNF-alpha (2 µg/mouse, N = 7) increased (P &lt; 0.01) GR and delayed GIT, mimicking the effect of LPS (50 µg/mouse). During early endotoxemia (1.5 h after LPS), inhibition of inducible NO synthase (iNOS) by a selective inhibitor, 1400 W (150 µg/mouse, N = 11), but not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endotoxin; Inducible nitric oxide synthase; Tumor necrosis factor-alpha; Gastric emptying; Gastrointestinal transit; Lipopolysaccharide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100006

Dietary fiber, energy intake and nutritional status during the treatment of children with chronic constipation
Provedor de dados: 56
Autores: Speridião,P.G.L.; Tahan,S.; Fagundes-Neto,U.; Morais,M.B.
The present prospective study was carried out to determine dietary fiber and energy intake and nutritional status of children during the treatment of chronic constipation. Twenty-five patients aged 2 to 12 years with chronic constipation were submitted to clinical evaluation, assessment of dietary patterns, and anthropometry before and after 45 and 90 days of treatment. The treatment of chronic constipation included rectal disimpaction, ingestion of mineral oil and diet therapy. The standardized diet prescribed consisted of regular food without a fiber supplement and met the nutrient requirements according to the recommended daily allowance. The fiber content was 9.0 to 11.9 g for patients aged less than 6 years and 12.0 to 18.0 g for patients older than 6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Constipation; Anthropometry; Nutritional status; Energy intake; Dietary fiber.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600011

CorrectionBraz J Med Biol Res 2011; 44(8): 754-761Semi-automatic measurement of visual verticality perception in humans reveals a new category of visual field dependency
Provedor de dados: 56
Autores: Kaleff,C.R.; Aschidamini,C.; Baron,J.; Di Leone,C.N.; Canavarro,S.; Vargas,C.D.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000013

An update on the physiology of two- and three-toed sloths
Provedor de dados: 56
Autores: Gilmore,D.P.; Da-Costa,C.P.; Duarte,D.P.F.
Physiological and pharmacological research undertaken on sloths during the past 30 years is comprehensively reviewed. This includes the numerous studies carried out upon the respiratory and cardiovascular systems, anesthesia, blood chemistry, neuromuscular responses, the brain and spinal cord, vision, sleeping and waking, water balance and kidney function and reproduction. Similarities and differences between the physiology of sloths and that of other mammals are discussed in detail.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xenarthra; Sloths; Bradypus; Choloepus; Physiology.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200001

The vulnerability of Brazilian female prisoners to HIV infection
Provedor de dados: 56
Autores: Strazza,L.; Azevedo,R.S.; Carvalho,H.B.; Massad,E.
The purpose of the present study was to determine the vulnerability of women in prison to HIV infection. The study was carried out from August to October 2000 in a São Paulo State Penitentiary, where 299 female prisoners were serving time. We interviewed and obtained a blood sample from 290 females who agreed to enter the study. Sera were tested for the presence of antibodies to HIV, hepatitis C virus (HCV) and syphilis and the odds ratio (OR) was calculated for variables related to HIV positivity on the basis of a questionnaire. The overall prevalence data were: 13.9% for HIV (37 of 267), 22.8% for syphilis (66 of 290), and 16.2% for HCV (47 of 290). Sexual partnership variables were significantly related to HIV infection. These included HIV-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; HCV; Syphilis; Vulnerable populations; Women; Prisoners.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500020

Clinical features of panic patients sensitive to hyperventilation or breath-holding methods for inducing panic attacks
Provedor de dados: 56
Autores: Nardi,A.E.; Valença,A.M.; Lopes,F.L.; Nascimento,I.; Mezzasalma,M.A.; Zin,W.A.
Our aim was to compare the clinical features of panic disorder (PD) patients sensitive to hyperventilation or breath-holding methods of inducing panic attacks. Eighty-five PD patients were submitted to both a hyperventilation challenge test and a breath-holding test. They were asked to hyperventilate (30 breaths/min) for 4 min and a week later to hold their breath for as long as possible, four times with a 2-min interval. Anxiety scales were applied before and after the tests. We selected the patients who responded with a panic attack to just one of the tests, i.e., those who had a panic attack after hyperventilating (HPA, N = 24, 16 females, 8 males, mean age ± SD = 38.5 ± 12.7 years) and those who had a panic attack after breath holding (BHPA, N = 20, 11...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety disorder; Challenge tests; Panic disorder; Hyperventilation; Breath holding.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200013

p.F508del in a heterogeneous cystic fibrosis population from Minas Gerais, Brazil
Provedor de dados: 56
Autores: Vidigal,P.V.T.; Reis,F.J.C.; Boson,W.L.M.; De Marco,L.A.; Brasileiro-Filho,G.
Cystic fibrosis (CF) is the most common autosomal recessive disease of the Caucasian population. Among the various CF mutations, p.F508del is the most frequent, accounting for two-thirds of the global CF chromosomes, although showing great variability among populations. We have studied 115 unrelated CF patients from a mixed population of Minas Gerais (Brazil). To evaluate part of the DNA sequence of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, blood DNA was obtained and PCR was performed using two pairs of primers that anneal to exons 10 and 24 of the CFTR gene. The PCR product was then submitted to automatic sequencing using the ABI PRISM 310 Genetic Analyzer. The p.F508del mutation was found in 50 (21.7%) of 230 unrelated CF...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cystic fibrosis; P.F508del; Cystic fibrosis transmembrane conductance regulator.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800001

Capecitabine maintenance therapy in patients with recurrent or metastatic breast cancer
Provedor de dados: 56
Autores: Si,W.; Zhu,Y.Y.; Li,Y.; Gao,P.; Han,C.; You,J.H.; Linghu,R.X.; Jiao,S.C.; Yang,J.L.
Our objective was to investigate the efficacy and safety of capecitabine maintenance therapy (CMT) after capecitabine-based combination chemotherapy in patients with metastatic breast cancer. The clinical data of 139 metastatic breast cancer patients treated from March 2008 to May 2012 with capecitabine-based combination chemotherapy were retrospectively analyzed. When initial disease control was achieved by the combination chemotherapy, we used CMT for 50 patients, while 37 patients were treated with a different (non-CMT) maintenance therapy. We compared time to progression (TTP), objective response rate, disease control rate, clinical benefit rate, and safety of the two groups, and a sub-group analysis was performed according to pathological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capecitabine; Maintenance therapy; Metastatic breast cancer.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201074

Effect of lipoarabinomannan from Mycobacterium avium subsp avium in Freund’s incomplete adjuvant on the immune response of cattle
Provedor de dados: 56
Autores: Colavecchia,S.B.; Jolly,A.; Fernández,B.; Fontanals,A.M.; Fernández,E.; Mundo,S.L.
The aim of the present study was to determine whether lipoarabinomannan (LAM), in combination with Freund’s incomplete adjuvant (FIA), was able to improve cell-mediated and antibody-mediated immune responses against ovalbumin (OVA) in cattle. Twenty-three calves were assigned to four treatment groups, which were subcutaneously immunized with either OVA plus FIA, OVA plus FIA and LAM from Mycobacterium avium subsp avium, FIA plus LAM, or FIA alone. Lymphoproliferation, IFN-γ production and cell subpopulations on peripheral blood mononuclear cells before and 15 days after treatment were evaluated. Delayed hypersensitivity was evaluated on day 57. Specific humoral immune response was measured by ELISA. Inoculation with LAM induced higher levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipoarabinomannan; Cattle; Immunomodulation; Mycobacterium avium subsp avium.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200008

Effects of lead and/or zinc exposure during the second stage of rapid postnatal brain growth on delta-aminolevulinate dehydratase and negative geotaxis of suckling rats
Provedor de dados: 56
Autores: Goulart,E.C.; Pereira,C.A.T.; Garcia,R.C.; Giacomelli,M.B.O.; Rodrigues,A.L.S.
Lead has been shown to produce cognitive and motor deficits in young rats that could be mediated, at least in part, by inhibition of the zinc-containing heme biosynthetic enzyme delta-aminolevulinate dehydratase (ALA-D). In the present study we investigated the effects of lead and/or zinc treatment during the second stage of rapid postnatal brain development on brain, kidney and blood ALA-D specific activity, as well as the negative geotaxis behavior of rats. Eight-day-old Wistar rats were injected intraperitoneally with saline, lead acetate (8 mg/kg) and/or zinc chloride (2 mg/kg) daily for five consecutive days. Twenty-four hours after treatment, ALA-D activity was determined in the absence and presence of DL-dithiothreitol (DTT). The negative geotaxis...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lead acetate; Zinc chloride; Delta-aminolevulinate dehydratase; Negative geotaxis; Postnatal brain growth.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600014

Signal transduction induced in Trypanosoma cruzi metacyclic trypomastigotes during the invasion of mammalian cells
Provedor de dados: 56
Autores: Yoshida,N.; Favoreto Jr.,S.; Ferreira,A.T.; Manque,P.M.
Penetration of Trypanosoma cruzi into mammalian cells depends on the activation of the parasite's protein tyrosine kinase and on the increase in cytosolic Ca2+ concentration. We used metacyclic trypomastigotes, the T. cruzi developmental forms that initiate infection in mammalian hosts, to investigate the association of these two events and to identify the various components of the parasite signal transduction pathway involved in host cell invasion. We have found that i) both the protein tyrosine kinase activation, as measured by phosphorylation of a 175-kDa protein (p175), and Ca2+ mobilization were induced in the metacyclic forms by the HeLa cell extract but not by the extract of T. cruzi-resistant K562 cells; ii) treatment of parasites with the tyrosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Signal transduction; Metacyclic trypomastigotes; Protein kinase; Calcium response; Cell invasion.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300003

Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations
Provedor de dados: 56
Autores: Petrucci Júnior,O.; Oliveira,P.P.M.; Carmo,M.R.; Vieira,R.W.; Braile,D.M.
In the present study we standardized an experimental model of parabiotic circulation of isolated pig heart. The isolated heart was perfused with arterial blood from a second animal as support and submitted to regional ischemia for 30 min, followed by total ischemia for 90 min and reperfusion for 90 min. Parameters for measurement of ventricular performance using different indices measured directly or indirectly from intraventricular pressure were defined as: maximum peak pressure, final diastolic pressure, pressure developed, first derivative of maximum pressure (dP/dt max), first derivative of minimum pressure (dP/dt min), systolic stress of the left ventricle (sigmas), and maximum elastance of the left ventricle. Isolated hearts subjected to regional and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolated heart; Ischemia; Parabiotic circulation; Reperfusion; Model.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500013

Epstein-Barr virus infection and gastric carcinoma in São Paulo State, Brazil
Provedor de dados: 56
Autores: Lopes,L.F.; Bacchi,M.M.; Elgui-de-Oliveira,D.; Zanati,S.G.; Alvarenga,M.; Bacchi,C.E.
Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and most people have serological evidence of previous viral infection at adult age. EBV is associated with infectious mononucleosis and human cancers, including some lymphomas and gastric carcinomas. Although EBV was first reported in lymphoepithelioma-like gastric carcinoma, the virus was also found in conventional adenocarcinomas. In the present study, 53 gastric carcinomas diagnosed in São Paulo State, Brazil, were evaluated for EBV infection by non-isotopic in situ hybridization with a biotinylated probe (Biotin-AGACACCGTCCTCACCACCC GGGACTTGTA) directed to the viral transcript EBER-I, which is actively expressed in EBV latently infected cells. EBV infection was found in 6 of 53 (11.32%) gastric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epstein-Barr virus; Gastric carcinoma; In situ hybridization; Brazil.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100016

Hydrogen sulfide postconditioning protects isolated rat hearts against ischemia and reperfusion injury mediated by the JAK2/STAT3 survival pathway
Provedor de dados: 56
Autores: Luan,Heng-Fei; Zhao,Zhi-Bin; Zhao,Qi-Hong; Zhu,Pin; Xiu,Ming-Yu; Ji,Yong.
The JAK2/STAT3 signal pathway is an important component of survivor activating factor enhancement (SAFE) pathway. The objective of the present study was to determine whether the JAK2/STAT3 signaling pathway participates in hydrogen sulfide (H2S) postconditioning, protecting isolated rat hearts from ischemic-reperfusion injury. Male Sprague-Dawley rats (230-270 g) were divided into 6 groups (N = 14 per group): time-matched perfusion (Sham) group, ischemia/reperfusion (I/R) group, NaHS postconditioning group, NaHS with AG-490 group, AG-490 (5 µM) group, and dimethyl sulfoxide (DMSO; &lt;0.2%) group. Langendorff-perfused rat hearts, with the exception of the Sham group, were subjected to 30 min of ischemia followed by 90 min of reperfusion after 20 min of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrogen sulfide; JAK2/STAT3; Apoptosis; Postconditioning; Ischemia/reperfusion injury.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000003

Factors associated with penicillin-nonsusceptible pneumococcal infections in Brazil
Provedor de dados: 56
Autores: Levin,A.S.; Sessegolo,J.F.; Teixeira,L.M.; Barone,A.A.
Resistance of Streptococcus pneumoniae is a worldwide, growing problem. Studies of factors associated with resistance to penicillin have not been conducted in Brazil. The objective of the present study was to evaluate factors associated with infection by S. pneumoniae not susceptible to penicillin. A prevalence study was conducted including all patients with a positive culture for S. pneumoniae in a hospital from July 1991 to December 1992 and the year 1994. Of 165 patients identified, 139 were considered to have clinically relevant infections and 88% of them had invasive infections. All infections were community acquired and consisted of pneumonia (44%) and of central nervous system (19%), pelvic or abdominal (12%), upper airway or ocular (12%), primary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Penicillin susceptibility; Risk factors; Prevalence study.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600017

Combating oncogene activation associated with retrovirus-mediated gene therapy of X-linked severe combined immunodeficiency
Provedor de dados: 56
Autores: Strauss,B.E.; Costanzi-Strauss,E.
A successful gene therapy clinical trial that also encountered serious adverse effects has sparked extensive study and debate about the future directions for retrovirus-mediated interventions. Treatment of X-linked severe combined immunodeficiency with an oncoretrovirus harboring a normal copy of the gc gene was applied in two clinical trials, essentially curing 13 of 16 infants, restoring a normal immune system without the need for additional immune-related therapies. Approximately 3 years after their gene therapy, tragically, 3 of these children, all from the same trial, developed leukemia as a result of this experimental treatment. The current understanding of the mechanism behind this leukemogenesis involves three critical and cooperating factors,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Retrovirus; Insertional mutagenesis; Severe combined immune deficiency; Gene therapy; Adverse event; Clinical trial.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500002

Periodic mechanical stress activates MEK1/2-ERK1/2 mitogenic signals in rat chondrocytes through Src and PLCγ1
Provedor de dados: 56
Autores: Ren,Kewei; Ma,Yimin; Huang,Yumin; Liang,Wenwei; Liu,Feng; Wang,Qing; Cui,Weiding; Liu,Zhengyu; Yin,Guoyong; Fan,Weimin.
The mitogenic effects of periodic mechanical stress on chondrocytes have been studied extensively but the mechanisms whereby chondrocytes sense and respond to periodic mechanical stress remain a matter of debate. We explored the signal transduction pathways of chondrocyte proliferation and matrix synthesis under periodic mechanical stress. In particular, we sought to identify the role of the MEK1/2-ERK1/2 signaling pathway in chondrocyte proliferation and matrix synthesis following cyclic physiologic mechanical compression. Under periodic mechanical stress, both rat chondrocyte proliferation and matrix synthesis were significantly increased (P &lt; 0.05) and were associated with increases in the phosphorylation of Src, PLCγ1, MEK1/2, and ERK1/2 (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Periodic mechanical stress; Chondrocyte proliferation and matrix synthesis; Src; PLCγ1; MEK1/2-ERK1/2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200006

Dietary sodium intake induced myenteric neuron hypertrophy in Wistar rats
Provedor de dados: 56
Autores: De Souza,R.R.; Gama,E.F.; Silva,R.D.A.; Heimann,J.C.; Maifrino,L.B.M.; Liberti,E.A.
In the present study we investigated the effect of salt intake on myenteric neuron size of the colon of adult male Wistar rats. The animals were placed on either a high-salt (HS; 8%; 12 animals) or a low-salt diet (LS; 0.15%; 12 animals) for 15 or 52 weeks and blood pressure was measured. The sizes of myenteric neurons of the distal colon from both groups were measured. No difference in neuron size was observed between the HS and LS groups after 15 weeks. After 52 weeks on HS, neuron size was increased (P&amp;lt;0.005) when compared with the LS group. The rats also presented hypertension, which was significantly different at 52 weeks (142 ± 11 vs 119 ± 7 mmHg). These results suggest that a long time on an HS diet can significantly increase myenteric nerve...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myenteric plexus; Neuron size; Cell growth; Sodium intake.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700017

Possible involvement of A1 receptors in the inhibition of gonadotropin secretion induced by adenosine in rat hemipituitaries in vitro
Provedor de dados: 56
Autores: Picanço-Diniz,D.L.W.; Valença,M.M.; Favaretto,A.L.V.; McCann,S.M.; Antunes-Rodrigues,J.
We investigated the participation of A1 or A2 receptors in the gonadotrope and their role in the regulation of LH and FSH secretion in adult rat hemipituitary preparations, using adenosine analogues. A dose-dependent inhibition of LH and FSH secretion was observed after the administration of graded doses of the R-isomer of phenylisopropyladenosine (R-PIA; 1 nM, 10 nM, 100 nM, 1 µM and 10 µM). The effect of R-PIA (10 nM) was blocked by the addition of 8-cyclopentyltheophylline (CPT), a selective A1 adenosine receptor antagonist, at the dose of 1 µM. The addition of an A2 receptor-specific agonist, 5-N-methylcarboxamidoadenosine (MECA), at the doses of 1 nM to 1 µM had no significant effect on LH or FSH secretion, suggesting the absence of this receptor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenosine; LH; FSH; A1 receptor; Anterior pituitary.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900017

Editorial
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100001

The molecular basis of selective permeability of connexins is complex and includes both size and charge
Provedor de dados: 56
Autores: Nicholson,B.J.; Weber,P.A.; Cao,F.; Chang,H.-C.; Lampe,P.; Goldberg,G.
Although gap junction channels are still widely viewed as large, non-specific pores connecting cells, the diversity in the connexin family has led more attention to be focused on their permeability characteristics. We summarize here the current status of these investigations, both published and on-going, that reveal both charge and size selectivity between gap junction channels composed of different connexins. In particular, this review will focus on quantitative approaches that monitor the expression level of the connexins, so that it is clear that differences that are seen can be attributed to channel properties. The degree of selectivity that is observed is modest compared to other channels, but is likely to be significant for biological molecules that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intercellular channels; Permeability; Channel selectivity; Gap junctions.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400002

Hyperventilation in panic disorder patients and healthy first-degree relatives
Provedor de dados: 56
Autores: Nardi,A.E.; Valença,A.M.; Nascimento,I.; Mezzasalma,M.A.; Lopes,F.L.; Zin,W.A.
Our aim was to observe the induction of panic attacks by a hyperventilation challenge test in panic disorder patients (DSM-IV) and their healthy first-degree relatives. We randomly selected 25 panic disorder patients, 31 healthy first-degree relatives of probands with panic disorder and 26 normal volunteers with no family history of panic disorder. All patients had no psychotropic drugs for at least one week. They were induced to hyperventilate (30 breaths/min) for 4 min and anxiety scales were applied before and after the test. A total of 44.0% (N = 11) panic disorder patients, 16.1% (N = 5) of first-degree relatives and 11.5% (N = 3) of control subjects had a panic attack after hyperventilating (chi² = 8.93, d.f. = 2, P = 0.011). In this challenge test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panic attacks; Respiration; Anxiety disorder; Ventilation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100009

Congenital ventricular septal defects and prenatal exposure to cyclooxygenase inhibitors
Provedor de dados: 56
Autores: Burdan,F.; Szumilo,J.; Dudka,J.; Korobowicz,A.; Klepacz,R.
Ventricular septal defects (VSDs) are common congenital abnormalities which have been reported to be associated with maternal fever and various environmental factors. The aim of the present study was to evaluate the effect of prenatal exposure to cyclooxygenase (COX) inhibitors on heart defects. A retrospective statistical analysis was performed using data collected in our laboratory during various teratological studies carried out on albino CRL:(WI)WUBR Wistar strain rats from 1997 to 2004. The observations were compared with concurrent and historic control data, as well as findings from other developmental toxicological studies with selective and nonselective COX-2 inhibitors. Despite the lack of significant differences in the frequency of VSDs between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclooxygenase; Cyclooxygenase inhibitor; Nonsteroidal anti-inflammatory drug; Ventricular septal defect.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700011

Noninvasive intracranial pressure monitoring for HIV-associated cryptococcal meningitis
Provedor de dados: 56
Autores: Bollela,V.R.; Frigieri,G.; Vilar,F.C.; Spavieri Jr,D.L.; Tallarico,F.J.; Tallarico,G.M.; Andrade,R.A.P.; de Haes,T.M.; Takayanagui,O.M.; Catai,A.M.; Mascarenhas,S.
Mortality and adverse neurologic sequelae from HIV-associated cryptococcal meningitis (HIV-CM) remains high due to raised intracranial pressure (ICP) complications. Cerebrospinal fluid (CSF) high opening pressure occurs in more than 50% of HIV-CM patients. Repeated lumbar puncture with CSF drainage and external lumbar drainage might be required in the management of these patients. Usually, there is a high grade of uncertainty and the basis for clinical decisions regarding ICP hypertension tends to be from clinical findings (headache, nausea and vomiting), a low Glasgow coma scale score, and/or fundoscopic papilledema. Significant neurological decline can occur if elevated CSF pressures are inadequately managed. Various treatment strategies to address...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Brain diseases; Intracranial hypertension; AIDS-related opportunistic infections; Cryptococcus neoformans; Monitoring.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900501

Acute lead-induced vasoconstriction in the vascular beds of isolated perfused rat tails is endothelium-dependent
Provedor de dados: 56
Autores: Silveira,E.A.; Lizardo,J.H.F.; Souza,L.P.; Stefanon,I.; Vassallo,D.V.
Chronic lead exposure induces hypertension in humans and animals, affecting endothelial function. However, studies concerning acute cardiovascular effects are lacking. We investigated the effects of acute administration of a high concentration of lead acetate (100 µΜ) on the pressor response to phenylephrine (PHE) in the tail vascular bed of male Wistar rats. Animals were anesthetized with sodium pentobarbital and heparinized. The tail artery was dissected and cannulated for drug infusion and mean perfusion pressure measurements. Endothelium and vascular smooth muscle relaxation were tested with acetylcholine (5 µg/100 µL) and sodium nitroprusside (0.1 µg/100 µL), respectively, in arteries precontracted with 0.1 µM PHE. Concentration-response curves to PHE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lead acetate; Vascular reactivity; Endothelium; Hypertension.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500011

Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
Provedor de dados: 56
Autores: Sá,V.K. de; Rocha,T.P.; Moreira,AL.; Soares,F.A.; Takagaki,T.; Carvalho,L.; Nicholson,A.G.; Capelozzi,V.L.
We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, adenomatous hyperplasia, severe dysplasia, squamous cell carcinoma and adenocarcinoma. Matched lung parenchyma tissue samples included 25 squamous cell carcinomas and 31 adenocarcinomas. Immunohistochemistry was performed to analyze for the distribution of hyaluronidase (Hyal)-1 and −3, and hyaluronan synthases (HAS)-1, −2, and −3. Hyal-1 showed significantly higher expression in basal cell hyperplasia than in moderate dysplasia (P=0.01), atypical adenomatous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronidases and hyaluronan synthases; Lung cancer; Pre-neoplastic lung/bronchial lesions; Immunohistochemistry; Morphometry; Prognosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101039

Connexin domains relevant to the chemical gating of gap junction channels
Provedor de dados: 56
Most cells exchange ions and small metabolites via gap junction channels. These channels are made of two hemichannels (connexons), each formed by the radial arrangement of six connexin (Cx) proteins. Connexins span the bilayer four times (M1-M4) and have both amino- and carboxy-termini (NT, CT) at the cytoplasmic side of the membrane, forming two extracellular loops (E1, E2) and one inner (IL) loop. The channels are regulated by gates that close with cytosolic acidification (e.g., CO2 treatment) or increased calcium concentration, possibly via calmodulin activation. Although gap junction regulation is still unclear, connexin domains involved in gating are being defined. We have recently focused on the CO2 gating sensitivity of Cx32, Cx38 and various...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cell-cell interaction; Cell junctions; Gap junctions; Calcium; Calmodulin; Membrane channels.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500003

In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae
Provedor de dados: 56
Autores: Formagio,A.S.N.; Vieira,M.C.; Volobuff,C.R.F.; Silva,M.S.; Matos,A.I.; Cardoso,C.A.L.; Foglio,M.A.; Carvalho,J.E.
The aim of this research was to investigate the antiproliferative and anticholinesterase activities of 11 extracts from 5 Annonaceae species in vitro. Antiproliferative activity was assessed using 10 human cancer cell lines. Thin-layer chromatography and a microplate assay were used to screen the extracts for acetylcholinesterase (AchE) inhibitors using Ellman's reagent. The chemical compositions of the active extracts were investigated using high performance liquid chromatography. Eleven extracts obtained from five Annonaceae plant species were active and were particularly effective against the UA251, NCI-470 lung, HT-29, NCI/ADR, and K-562 cell lines with growth inhibition (GI50) values of 0.04-0.06, 0.02-0.50, 0.01-0.12, 0.10-0.27, and 0.02-0.04 µg/mL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annona; Duguetia; Antiproliferative; Anticholinesterase activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400308

The mechanism of gentisic acid-induced relaxation of the guinea pig isolated trachea: the role of potassium channels and vasoactive intestinal peptide receptors
Provedor de dados: 56
Autores: Cunha,J.F.; Campestrini,F.D.; Calixto,J.B.; Scremin,A.; Paulino,N.
We examined some of the mechanisms by which the aspirin metabolite and the naturally occurring metabolite gentisic acid induced relaxation of the guinea pig trachea in vitro. In preparations with or without epithelium and contracted by histamine, gentisic acid caused concentration-dependent and reproducible relaxation, with mean EC50 values of 18 µM and Emax of 100% (N = 10) or 20 µM and Emax of 92% (N = 10), respectively. The relaxation caused by gentisic acid was of slow onset in comparison to that caused by norepinephrine, theophylline or vasoactive intestinal peptide (VIP). The relative rank order of potency was: salbutamol 7.9 &gt; VIP 7.0 &gt; gentisic acid 4.7 &gt; theophylline 3.7. Gentisic acid-induced relaxation was markedly reduced (24 ± 7.0, 43...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gentisic acid; Potassium channels; Vasoactive intestinal peptide; VIP; Trachea; Smooth muscle.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300013

T cell subsets and immunoglobulin G levels are associated with the infection status of systemic lupus erythematosus patients
Provedor de dados: 56
Autores: Wu,Lifen; Wang,Xinru; Chen,Fenghua; Lv,Xing; Sun,Wenwen; Guo,Ying; Hou,Hou; Ji,Haiyan; Wei,Wei; Gong,Lu.
Systemic lupus erythematosus (SLE) is a chronic, autoimmune disorder that affects nearly all organs and tissues. As knowledge about the mechanism of SLE has increased, some immunosuppressive agents have become routinely used in clinical care, and infections have become one of the direct causes of mortality in SLE patients. To identify the risk factors indicative of infection in SLE patients, a case control study of our hospital's medical records between 2011 and 2013 was performed. We reviewed the records of 117 SLE patients with infection and 61 SLE patients without infection. Changes in the levels of T cell subsets, immunoglobulin G (IgG), complement C3, complement C4, globulin, and anti-double-stranded DNA (anti-ds-DNA) were detected. CD4+ and CD4+/CD8+...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Infection; T cell subsets; Immunoglobulin G.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200610

Hind limb ischemic preconditioning induces an anti-inflammatory response by remote organs in rats
Provedor de dados: 56
Autores: Souza Filho,M.V.P.; Loiola,R.T.; Rocha,E.L.; Simão,A.F.L.; Gomes,A.S.; Souza,M.H.L.P.; Ribeiro,R.A.
Ischemic preconditioning (IPC), a strategy used to attenuate ischemia-reperfusion injury, consists of brief ischemic periods, each followed by reperfusion, prior to a sustained ischemic insult. The purpose of the present study was to evaluate the local and systemic anti-inflammatory effects of hind limb IPC in male Wistar rat (200-250 g) models of acute inflammation. IPC was induced with right hind limb ischemia for 10 min by placing an elastic rubber band tourniquet on the proximal part of the limb followed by 30 min of reperfusion. Groups (N = 6-8) were submitted to right or left paw edema (PE) with carrageenan (100 µg) or Dextran (200 µg), hemorrhagic cystitis with ifosfamide (200 mg/kg, ip) or gastric injury (GI) with indomethacin (20 mg/kg, vo)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemic preconditioning; Carrageenan; Dextran; Ifosfamide; Indomethacin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000008

Lipoic acid, but not tempol, preserves vascular compliance and decreases medial calcification in a model of elastocalcinosis
Provedor de dados: 56
Autores: Bassi,E.; Liberman,M.; Martinatti,M.K.; Bortolotto,L.A.; Laurindo,F.R.M.
Vascular calcification decreases compliance and increases morbidity. Mechanisms of this process are unclear. The role of oxidative stress and effects of antioxidants have been poorly explored. We investigated effects of the antioxidants lipoic acid (LA) and tempol in a model of atherosclerosis associated with elastocalcinosis. Male New Zealand white rabbits (2.5-3.0 kg) were fed regular chow (controls) or a 0.5% cholesterol (chol) diet+104 IU/day vitamin D2 (vitD) for 12 weeks, and assigned to treatment with water (vehicle, n=20), 0.12 mmol·kg-1·day-1 LA (n=11) or 0.1 mmol·kg-1·day-1 tempol (n=15). Chol+vitD-fed rabbits developed atherosclerotic plaques associated with expansive remodeling, elastic fiber disruption, medial calcification, and increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Vascular calcification; Atherosclerosis; Oxidative stress; Lipoic acid.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200119

Effects of different levels of positive airway pressure on breathing pattern and heart rate variability after coronary artery bypass grafting surgery
Provedor de dados: 56
Autores: Pantoni,C.B.F.; Di Thommazo,L.; Mendes,R.G.; Catai,A.M.; Luzzi,S.; Amaral Neto,O.; Borghi-Silva,A.
The application of continuous positive airway pressure (CPAP) produces important hemodynamic alterations, which can influence breathing pattern (BP) and heart rate variability (HRV). The aim of this study was to evaluate the effects of different levels of CPAP on postoperative BP and HRV after coronary artery bypass grafting (CABG) surgery and the impact of CABG surgery on these variables. Eighteen patients undergoing CABG were evaluated postoperatively during spontaneous breathing (SB) and application of four levels of CPAP applied in random order: sham (3 cmH2O), 5 cmH2O, 8 cmH2O, and 12 cmH2O. HRV was analyzed in time and frequency domains and by nonlinear methods and BP was analyzed in different variables (breathing frequency, inspiratory tidal volume,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Continuous positive airway pressure; Cardiovascular system; Autonomic nervous system; Ventilation; Coronary artery bypass.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100006

Expression of CHPF modulates cell proliferation and invasion in lung cancer
Provedor de dados: 56
Autores: Cao,Chengsong; Liu,Yong; Wang,Qun; Zhao,Jing; Shi,Ming; Zheng,Junnian.
Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CHPF; Lung cancer; Proliferation; Invasion; TCGA; Affymetrix Gene Chip.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500610

Treatment of hemorrhagic shock with hypertonic saline solution modulates the inflammatory response to live bacteria in lungs
Provedor de dados: 56
Autores: Fernandes,C.I.; Llimona,F.; Godoy,L.C.; Negri,E.M.; Pontieri,V.; Moretti,A.I.; Fernandes,T.R.; Soriano,F.G.; Velasco,I.T.; Souza,H.P.
Shock and resuscitation render patients more susceptible to acute lung injury due to an exacerbated immune response to subsequent inflammatory stimuli. To study the role of innate immunity in this situation, we investigated acute lung injury in an experimental model of ischemia-reperfusion (I-R) followed by an early challenge with live bacteria. Conscious rats (N = 8 in each group) were submitted to controlled hemorrhage and resuscitated with isotonic saline (SS, 0.9% NaCl) or hypertonic saline (HS, 7.5% NaCl) solution, followed by intratracheal or intraperitoneal inoculation of Escherichia coli. After infection, toll-like receptor (TLR) 2 and 4 mRNA expression was monitored by RT-PCR in infected tissues. Plasma levels of tumor necrosis factor α and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toll-like receptors; Immune system; Pneumonia; Escherichia coli; Neutrophils.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000004

Effect of a cholesterol-rich diet on the metabolism of the free and esterified cholesterol components of a nanoemulsion that resembles LDL in rabbits
Provedor de dados: 56
Autores: Padoveze,A.F.; Maniero,F.; Oliveira,T.V.; Vitorio,T.S.; Couto,R.D.; Maranhão,R.C.
We have shown that the free cholesterol (FC) and the cholesteryl ester (CE) moieties of a nanoemulsion with lipidic structure resembling low-density lipoproteins show distinct metabolic fate in subjects and that this may be related to the presence of dyslipidemia and atherosclerosis. The question was raised whether induction of hyperlipidemia and atherosclerosis in rabbits would affect the metabolic behavior of the two cholesterol forms. Male New Zealand rabbits aged 4-5 months were allocated to a control group (N = 17) fed regular chow and to a 1% cholesterol-fed group (N = 13) during a 2-month period. Subsequently, the nanoemulsion labeled with ³H-FC and 14C-CE was injected intravenously for the determination of plasma kinetics and tissue uptake of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipoprotein kinetics; Free cholesterol; Hyperlipidemia; Rabbit atherosclerosis; Nanoemulsions; Cholesterol-rich diet.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200005

Trailing end-point phenotype antibiotic-sensitive strains of Candida albicans produce different amounts of aspartyl peptidases
Provedor de dados: 56
Autores: Braga-Silva,L.A.; Mesquita,D.G.A.; Ribeiro,M.D.; Carvalho,S.M.F.; Fracalanzza,S.E.L.; Santos,A.L.S.
Candida albicans is an opportunistic fungal pathogen that causes severe systemic infections in immunosuppressed individuals. C. albicans resistance to antifungal drugs is a severe problem in patients receiving prolonged therapy. Moreover, trailing yeast growth, which is defined as a resistant MIC after 48 h of incubation with triazole antifungal agents but a susceptible MIC after 24 h, has been noted in tests of antifungal susceptibility against some C. albicans isolates. In this context, we recently noticed this phenomenon in our routine susceptibility tests with fluconazole/itraconazole and C. albicans clinical isolates. In the present study, we investigated the production of cell-associated and secreted aspartyl peptidases (Saps) in six trailing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida albicans; Trailing growth; Secreted aspartyl peptidases; Sap2.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800013

Training graduate students to be teachers
Provedor de dados: 56
Autores: de-Macedo,D.V.; de-Paula,E.; Torres,B.B.
Pedagogic education of graduate students, when and where it exists, is restricted to theoretical courses or to the participation of the students as teachers' assistants. This model is essentially reproductive and offers few opportunities for any significant curriculum innovation. To open an opportunity for novelty we have introduced a new approach in "Biochemistry Teaching", a course included in the Biochemistry Graduate Program of the Biochemistry Department (Universidade Estadual de Campinas and Universidade de São Paulo). The content of the course consists of a) choosing the theme, b) selecting and organizing the topics, c) preparing written material, d) establishing the methodological strategies, e) planning the evaluation tools and, finally, f) as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pedagogic training; Tertiary-level education.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200002

Extracellular diffusion quantified by magnetic resonance imaging during rat C6 glioma cell progression
Provedor de dados: 56
Autores: Song,G.; Luo,T.; Dong,L.; Liu,Q.
Solution reflux and edema hamper the convection-enhanced delivery of the standard treatment for glioma. Therefore, a real-time magnetic resonance imaging (MRI) method was developed to monitor the dosing process, but a quantitative analysis of local diffusion and clearance parameters has not been assessed. The objective of this study was to compare diffusion into the extracellular space (ECS) at different stages of rat C6 gliomas, and analyze the effects of the extracellular matrix (ECM) on the diffusion process. At 10 and 20 days, after successful glioma modeling, gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA) was introduced into the ECS of rat C6 gliomas. Diffusion parameters and half-life of the reagent were then detected using MRI, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C6 glioma model; Extracellular space; Magnetic resonance imaging; Rats.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700610

Follicle profile and plasma gonadotropin concentration in pubertal female ponies
Provedor de dados: 56
Autores: Nogueira,G.P.
Twelve female ponies were examined daily for 30 days and classified as ovulating (OV; N = 6; 197 ± 6 kg) or prepubertal (PP; N = 6; 196 ± 9 kg). Follicles were detected by ultrasound and gonadotropins quantified by radioimmunoassay. The mean diameter of the largest follicles was significantly larger in OV (38 ± 1 mm) than in PP (26 ± 2 mm) but there was no difference between groups in the size of the second largest follicle. There were more small follicles (&lt;24 mm) in the PP than in the OV group, but PP fillies had a smaller number of follicles &gt;29 mm than the OV fillies. Follicle-stimulating hormone (FSH) levels did not differ between groups but PP fillies had lower luteinizing hormone (LH) peak (8 ± 1 ng/ml) and basal (4 ± 0.5 ng/ml) levels, lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Follicles; FSH; LH and prepubertal fillies.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600018

Inhibition of PKC-dependent extracellular Ca2+ entry contributes to the depression of contractile activity in long-term pressure-overloaded endothelium-denuded rat aortas
Provedor de dados: 56
Autores: Padilla,J.; López,R.M.; López,P.; Castillo,M.C.; Querejeta,E.; Ruiz,A.; Castillo,E.F.
We examined the contractile responsiveness of rat thoracic aortas under pressure overload after long-term suprarenal abdominal aortic coarctation (lt-Srac). Endothelium-dependent angiotensin II (ANG II) type 2 receptor (AT2R)-mediated depression of contractions to ANG II has been reported in short-term (1 week) pressure-overloaded rat aortas. Contractility was evaluated in the aortic rings of rats subjected to lt-Srac or sham surgery (Sham) for 8 weeks. ANG I and II levels and AT2R protein expression in the aortas of lt-Srac and Sham rats were also evaluated. lt-Srac attenuated the contractions of ANG II and phenylephrine in the aortas in an endothelium-independent manner. However, lt-Srac did not influence the transient contractions induced in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Long-term pressure overload; Rat aorta; Protein kinase C.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900789

Volatile cardioplegia: fast normothermic cardiac arrest induction and recovery with halothane in isolated rat hearts
Provedor de dados: 56
To study the effect of halothane as a cardioplegic agent, ten Wistar rats were anesthetized by ether inhalation and their hearts were perfused in a Langendorff system with Krebs-Henseleit solution (36oC; 90 cm H2O pressure). After a 15-min period for stabilization the control values for heart rate, force (T), dT/dt and coronary flow were recorded and a halothane-enriched solution (same temperature and pressure) was perfused until cardiac arrest was obtained. The same Krebs-Henseleit solution was reperfused again and the parameters studied were recorded after 1, 3, 5, 10, 20 and 30 min. Cardiac arrest occurred in all hearts during the first two min of perfusion with halothane-bubbled solution. One minute after reperfusion without halothane, the following...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cardioplegia; Myocardial; Protection; Halothane.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600009

Biomass smoke COPD has less tomographic abnormalities but worse hypoxemia compared with tobacco COPD
Provedor de dados: 56
Autores: Meneghini,A.C.; Koenigkam-Santos,M.; Pereira,M.C.; Tonidandel,P.R.; Terra-Filho,J.; Cunha,F.Q.; Menezes,M.B. de; Vianna,E.O.
Special attention has emerged towards biomass smoke-induced chronic obstructive pulmonary disease (COPD), providing new knowledge for prevention and therapeutic approach of non-smoker COPD patients. However, the understanding of biomass smoke COPD is still limited and somewhat controversial. The aim of the present study was to compare COPD exclusively caused by tobacco smoking with COPD exclusively caused by environmental or occupational exposures. For this cross-sectional study, COPD patients were recruited from outpatient clinics and formed two groups: non-smoker COPD group (n=16) with exposure to biomass smoke who did not smoke cigarette and tobacco smoker COPD group (n=15) with people who did not report biomass smoke exposure. Subjects underwent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Biomass; Tobacco.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500601

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells
Provedor de dados: 56
Autores: Hu,T.; Shi,J.; Jiao,X.; Zhou,J.; Yin,X.
Phosphatidylserine (PS) exposure occurs during the cell death program and fluorescein-labeled lactadherin permits the detection of PS exposure earlier than annexin V in suspended cell lines. Adherent cell lines were studied for this apoptosis-associated phenomenon to determine if PS probing methods are reliable because specific membrane damage may occur during harvesting. Apoptosis was induced in the human tongue squamous carcinoma cell line (Tca8113) and the adenoid cystic carcinoma cell line (ACC-2) by arsenic trioxide. Cells were harvested with a modified procedure and labeled with lactadherin and/or annexin V. PS exposure was localized by confocal microscopy and apoptosis was quantified by flow cytometry. The detachment procedure without trypsinization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adherent cells; Annexin V; Apoptosis; Flow cytometry; Lactadherin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900002

Partial recovery of erythrocyte glycogen in diabetic rats treated with phenobarbital
Provedor de dados: 56
Erythrocytes may play a role in glucose homeostasis during the postprandial period. Erythrocytes from diabetic patients are defective in glucose transport and metabolism, functions that may affect glycogen storage. Phenobarbital, a hepatic enzyme inducer, has been used in the treatment of patients with non-insulin-dependent diabetes mellitus (NIDDM), increasing the insulin-mediated glucose disposal. We studied the effects of phenobarbital treatment in vivo on glycemia and erythrocyte glycogen content in control and alloxan-diabetic rats during the postprandial period. In control rats (blood glucose, 73 to 111 mg/dl in femoral and suprahepatic veins) the erythrocyte glycogen content was 45.4 ± 1.1 and 39.1 ± 0.8 µg/g Hb (mean ± SEM, N = 4-6) in the femoral...
Palavras-chave: Erythrocytes; Glycogen; Phenobarbital; Diabetes mellitus; Erythrocyte metabolism; Alloxan.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500014

Cardiovascular control in experimental diabetes
Provedor de dados: 56
Autores: De Angelis,K.; Schaan,B.D.; Maeda,C.Y.; Dall'Ago,P.; Wichi,R.B.; Irigoyen,M.C.
Several studies have reported impairment in cardiovascular function and control in diabetes. The studies cited in this review were carried out from a few days up to 3 months after streptozotocin administration and were concerned with the control of the circulation. We observed that early changes (5 days) in blood pressure control by different peripheral receptors were maintained for several months. Moreover, the impairment of reflex responses observed after baroreceptor and chemoreceptor stimulation was probably related to changes in the efferent limb of the reflex arc (sympathetic and parasympathetic), but changes also in the central nervous system could not be excluded. Changes in renal sympathetic nerve activity during volume expansion were blunted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental diabetes; Arterial pressure; Autonomic control; Baroreflex; Chemoreflex; Cardiopulmonary reflex; Exercise training.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900010

Addressing conflicts of interest in the research paper: a societal demand in contemporary science?
Provedor de dados: 56
Autores: Vasconcelos,S.M.R.; Cassimiro,M.C.; Martins,M.F.M.; Palácios,M.
In the last decade, dialogue between science and society has found a forum in an increasing number of publications on topics such as public engagement with science and public trust in science. Concerning the latter, issues that include cases of research misconduct, accountability in research, and conflicts of interest (COIs) have shaped global discussions on the communication of science. In the publication setting, the perception that hiding COIs and/or not managing them well may affect public trust in the research record has grown among editors. We conducted a search for editorials addressing COIs between 1989 and 2011, using four major databases: Medline/PubMed, Embase, Scopus, and Web of Knowledge. We explored the content of these editorials and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conflicts of interest; Contemporary science; Science policy; Science education; Research integrity; Science and society.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201007

Analyzing gene expression profiles in dilated cardiomyopathy via bioinformatics methods
Provedor de dados: 56
Autores: Wang,Liming; Zhu,L.; Luan,R.; Wang,L.; Fu,J.; Wang,X.; Sui,L.
Dilated cardiomyopathy (DCM) is characterized by ventricular dilatation, and it is a common cause of heart failure and cardiac transplantation. This study aimed to explore potential DCM-related genes and their underlying regulatory mechanism using methods of bioinformatics. The gene expression profiles of GSE3586 were downloaded from Gene Expression Omnibus database, including 15 normal samples and 13 DCM samples. The differentially expressed genes (DEGs) were identified between normal and DCM samples using Limma package in R language. Pathway enrichment analysis of DEGs was then performed. Meanwhile, the potential transcription factors (TFs) and microRNAs (miRNAs) of these DEGs were predicted based on their binding sequences. In addition, DEGs were mapped...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dilated cardiomyopathy; Differentially expressed genes; Pathway enrichment analysis; Transcription factors; MicroRNAs; Small molecules.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000706

Agonistic-like responses from the torus semicircularis dorsalis elicited by GABA A blockade in the weakly electric fish Gymnotus carapo
Provedor de dados: 56
Autores: Duarte,T.T.; Corrêa,S.A.L.; Santana,U.J.; Pereira,A.S.F.; Hoffmann,A.
Findings by our group have shown that the dorsolateral telencephalon of Gymnotus carapo sends efferents to the mesencephalic torus semicircularis dorsalis (TSd) and that presumably this connection is involved in the changes in electric organ discharge (EOD) and in skeletomotor responses observed following microinjections of GABA A antagonist bicuculline into this telencephalic region. Other studies have implicated the TSd or its mammalian homologue, the inferior colliculus, in defensive responses. In the present study, we explore the possible involvement of the TSd and of the GABA-ergic system in the modulation of the electric and skeletomotor displays. For this purpose, different doses of bicuculline (0.98, 0.49, 0.245, and 0.015 mM) and muscimol (15.35...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electric fish; Torus semicircularis dorsalis; GABA-ergic system; Electromotor modulation; Agonistic behavior.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700013

Perturbation of EGF-induced MAP kinase activation by TGF-ß1
Provedor de dados: 56
Autores: Wenner,C.E.; Yan,S.
TGF-ß1 regulates both cellular growth and phenotypic plasticity important for maintaining a growth advantage and increased invasiveness in progressively malignant cells. Recent studies indicate that TGF-ß-1 stimulates the conversion of epitheliod to fibroblastoid phenotype which presumably leads to the inactivation of growth-inhibitory effects by TGF-ß1 (Portella et al. (1998) Cell Growth and Differentiation, 9: 393-404). Therefore, the investigation of TGF-ß1 signaling that leads to altered growth and migration may provide novel targets for the prevention of increased cell growth and invasion. Although much attention has been paid to TGF-ß1 responses in epithelial cells, the above studies suggest that examination of signal transduction pathways in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transforming growth factor; MAP kinase; Cyclin D1.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700004

Effects of pregnancy and protein-energy malnutrition on monooxygenase O-dealkylation activity in rat liver microsomes
Provedor de dados: 56
Autores: Kuriyama,S.N.; De-Oliveira,A.C.A.X.; Fidalgo-Neto,A.A.; Paumgartten,F.J.R.
Xenobiotic metabolism is influenced by a variety of physiological and environmental factors including pregnancy and nutritional status of the individual. Pregnancy has generally been reported to cause a depression of hepatic monooxygenase activities. Low-protein diets and protein-energy malnutrition have also been associated with a reduced activity of monooxygenases in nonpregnant animals. We investigated the combined effects of pregnancy and protein-energy malnutrition on liver monooxygenase O-dealkylation activity. On pregnancy day 0 rats were assigned at random to a group fed ad libitum (well-nourished, WN) or to a malnourished group (MN) which received half of the WN food intake (12 g/day). WN and MN rats were killed on days 0 (nonpregnant), 11 or 20...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytochrome P450; Monooxygenases; Xenobiotic metabolism; Undernutrition; Gestation; Biotransformation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100014

Time sequence of changes in the responsiveness of glycogen breakdown to adrenergic agonists in perfused liver of rats with insulin-induced hypoglycemia
Provedor de dados: 56
Autores: Vardanega-Peicher,M.; Lopes,G.; Lima,F.B.; Curi,R.; Nakano,L.C.; Bazotte,R.B.
The time-course changes of the responsiveness of glycogen breakdown to a- and ß-adrenergic agonists during insulin-induced hypoglycemia (IIH) were investigated. Blood glucose levels were decreased prior to the alteration in the hepatic responsiveness to adrenergic agonists. The activation of hepatic glucose production and glycogenolysis by phenylephrine (2 µM) and isoproterenol (20 µM) was decreased in IIH. The changes in the responsiveness of glycogen catabolism were first observed for isoproterenol and later for phenylephrine. Hepatic ß-adrenergic receptors showed a higher degree of adrenergic desensitization than did a-receptors. Liver glycogen synthase activity, glycogen content and the catabolic effect of dibutyryl cyclic AMP (the ß-receptor second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoglycemia; Adrenergic responsiveness; Insulin-induced hypoglycemia; Hepatic glucose production; Glycogenolysis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700011

Evaluation of a 1-h 75-g oral glucose tolerance test in the diagnosis of gestational diabetes
Provedor de dados: 56
Autores: Campos,M.A.A.; Reichelt,A.A.J.; Façanha,C.; Forti,A.C.; Schmidt,M.I.
In order to evaluate the performance of a 1-h 75-g oral glucose tolerance test (OGTT) for the diagnosis of gestational diabetes mellitus (GDM), a cohort of 4998 women, 20 years or older, without previous diabetes being treated in prenatal care clinics in Brazil answered a questionnaire and performed a 75-g OGTT including fasting, 1-h and 2-h glucose measurements between their 24th and 28th gestational weeks. Pregnancy outcomes were transcribed from medical registries. GDM was defined according to WHO criteria (fasting: ≥126 mg/dL; 2-h value: ≥140 mg/dL) and macrosomia as a birth weight equal to or higher than 4000 g. Areas under the receiver operator characteristic curve (AUC) were compared and diagnostic properties of various cut-off points were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gestational diabetes; Diagnosis; Macrosomia; 1-h OGTT; Sensitivity; Specificity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800008

Hemochromatosis (HFE) gene mutations in Brazilian chronic hemodialysis patients
Provedor de dados: 56
Autores: Perícole,F.V.; Alves,M.A.V.R.; Saad,S.T.O.; Costa,F.F.
Patients with chronic renal insufficiency (CRI) have reduced hemoglobin levels, mostly as a result of decreased kidney production of erythropoietin, but the relation between renal insufficiency and the magnitude of hemoglobin reduction has not been well defined. Hereditary hemochromatosis is an inherited disorder of iron metabolism. The importance of the association of hemochromatosis with treatment for anemia among patients with CRI has not been well described. We analyzed the frequency of the C282Y and H63D mutations in the HFE gene in 201 Brazilian individuals with CRI undergoing hemodialysis. The analysis of the effects of HFE mutations on iron metabolism and anemia with biochemical parameters was possible in 118 patients of this study (hemoglobin,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HFE; Chronic renal insufficiency; Anemia; Iron metabolism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900005

Important amino acid residues of potato plant uncoupling protein (StUCP)
Provedor de dados: 56
Autores: Jezek,P.; Costa,A.D.T.; Vercesi,A.E.
Chemical modifications were used to identify some of the functionally important amino acid residues of the potato plant uncoupling protein (StUCP). The proton-dependent swelling of potato mitochondria in K+-acetate in the presence of linoleic acid and valinomycin was inhibited by mersalyl (Ki = 5 µM) and other hydrophilic SH reagents such as Thiolyte MB, iodoacetate and 5,5'-dithio-bis-(2-nitrobenzoate), but not by hydrophobic N-ethylmaleimide. This pattern of inhibition by SH reagents was similar to that of brown adipose tissue uncoupling protein (UCP1). As with UCP1, the arginine reagent 2,3-butadione, but not N-ethylmaleimide or other hydrophobic SH reagents, prevented the inhibition of StUCP-mediated transport by ATP in isolated potato mitochondria or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant mitochondria; Uncoupling protein; Chemical modification; Mitochondrial swelling; Reconstitution.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200003

The effect of sun exposure on 25-hydroxyvitamin D concentrations in young healthy subjects living in the city of São Paulo, Brazil
Provedor de dados: 56
Autores: Maeda,S.S.; Kunii,I.S.; Hayashi,L.; Lazaretti-Castro,M.
The range of 25-hydroxyvitamin D (25OHD) concentration was determined in a young healthy population based on bone metabolism parameters and environmental and behavioral aspects. We studied 121 healthy young volunteers (49 men, 72 women) living in São Paulo (23º 34' south latitude) belonging to three occupational categories: indoor workers (N = 28), medical school students (N = 44), and resident physicians (N = 49). Fasting morning blood samples were collected once from each volunteer from August 2002 to February 2004, and 25OHD, total calcium, albumin, alkaline phosphatase, phosphorus, creatinine, intact parathyroid hormone, osteocalcin, and type I collagen carboxyterminal telopeptide were measured. Data are reported as means ± SD. Mean subject age was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 25-Hydroxyvitamin D; Parathyroid hormone; Occupational aspects; Seasonal influence; 25OHD and solar exposure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200009

Regulation of nephron acidification by corticosteroids
Provedor de dados: 56
The present paper reviews work from our laboratories evaluating the importance of adrenal cortical hormones in acidification by proximal and cortical distal tubules. Proximal acidification was determined by stationary microperfusion, and measurement of bicarbonate reabsorption using luminal pH determination was performed with H+-ion-sensitive microelectrodes. Rats were adrenalectomized (ADX) 48 h before the experiments, and corticosteroids (aldosterone (A), corticosterone (B), and 18-OH corticosterone (18-OH-B)) were injected intramuscularly 100 and 40 min before the experiments. In ADX rats stationary pH increased significantly to 7.03 as compared to sham-operated rats (6.78). Bicarbonate reabsorption decreased from 2.65 &amp;amp;plusmn; 0.18 in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aldosterone; Corticosterone; Bicarbonate reabsorption; Amiloride; Urine; PCO2.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400008

Patients with Duchenne and Becker muscular dystrophies are not more asymmetrical than healthy controls on timed performance of upper limb tasks
Provedor de dados: 56
Autores: Artilheiro,M.C.; Sá,C.S.C.; Fávero,F.M.; Caromano,F.A.; Voos,M.C.
This study aimed to investigate possible asymmetries and relationships between performance of dominant and non-dominant upper limbs (UL) in patients with Duchenne and Becker muscular dystrophies (DMD/BMD), to compare UL performance of patients and healthy subjects and to investigate the relationship between timed performance of UL and age, motor function and muscle strength in DMD/BMD patients. Sixteen patients with DMD and 3 with BMD were evaluated with Jebsen-Taylor Test (timed performance), Vignos scale and Dimension 3 of Motor Function Measure (motor function), and Medical Research Council scale (muscle strength) on a single session. ANOVA showed no asymmetry between dominant and non-dominant UL, except in the writing subtest, in patients and in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duchenne muscular dystrophy; Upper extremity; Evaluation; Physical therapy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800604

Inhibition of in vitro CO2 production and lipid synthesis by 2-hydroxybutyric acid in rat brain
Provedor de dados: 56
Autores: Silva,A.R.; Ruschel,C.; Helegda,C.; Wyse,A.T.S.; Wannmacher,C.M.D.; Wajner,M.; Dutra-Filho,C.S.
2-Hydroxybutyric acid appears at high concentrations in situations related to deficient energy metabolism (e.g., birth asphyxia) and also in inherited metabolic diseases affecting the central nervous system during neonatal development, such as "cerebral" lactic acidosis, glutaric aciduria type II, dihydrolipoyl dehydrogenase (E3) deficiency, and propionic acidemia. The present study was carried out to determine the effect of 2-hydroxybutyric acid at various concentrations (1-10 mM) on CO2 production and lipid synthesis from labeled substrates in cerebral cortex of 30-day-old Wistar rats in vitro. CO2 production was significantly inhibited (30-70%) by 2-hydroxybutyric acid in cerebral cortex prisms, in total homogenates and in the mitochondrial fraction. We...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lipid synthesis; Energy metabolism; Cerebral cortex; 2-hydroxybutyric acid.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500010

Effect of dietary linoleic acid on the progression of chronic renal failure in rats
Provedor de dados: 56
Autores: Gregório,S.M.P.; Lemos,C.C.S.; Caldas,M.L.; Bregman,R.
The role of linoleic acid in chronic renal failure (CRF) is controversial. In the present study 21 male Wistar rats submitted to 5/6 renal mass reduction (R) and 16 normal controls (C) were fed a supplement (S) or normal (N) linoleic acid diet for 60 days starting 10 days after CRF. As expected, serum creatinine, cholesterol and triglycerides (mean ± SEM) were higher in the CRF groups compared to the C groups (P&lt;0.05). The RS group presented lower cholesterol (84 ± 4 vs 126 ± 13 mg%) and triglyceride (88 ± 9 vs 132 ± 19 mg%) levels compared to the RN group. Proteinuria and kidney weight did not differ between CRF groups. Glomerular area increased 78% in RS and 100% in RN compared to control rats. Glomerular sclerosis index tended to be lower in RS (27%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney failure; Diet; Glomeruli; Linoleic acid.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500010

Hemodynamic and hormonal actions of adrenomedullin
Provedor de dados: 56
Autores: Nicholls,M.G.
Adrenomedullin, a 52-amino acid residue peptide, has numerous biological actions which are of potential importance to cardiovascular homeostasis, growth and development of cardiovascular tissues and bone, prevention of infection, and regulation of body fluid and electrolyte balance. Studies in man using intravenous infusion of the peptide have demonstrated that, at plasma levels detected after myocardial infarction or in heart failure, adrenomedullin reduces arterial pressure, increases heart rate and cardiac output, and activates the sympathetic and renin-angiotensin systems but suppresses aldosterone. The thresholds for these responses differ, being lower under some experimental circumstances for arterial pressure than for the other biological effects....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenomedullin; Hypertension; Heart failure; Aldosterone; Renin; Norepinephrine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800016

Association studies between -1185A/G von Willebrand factor gene polymorphism and coronary artery disease
Provedor de dados: 56
Autores: Simon,D.; Paludo,C.A.; Ghisleni,G.C.; Manfroi,W.C.; Roisenberg,I.
High levels of von Willebrand factor (vWF) have been associated with cardiovascular disease. The A allele of the -1185A/G polymorphism in the 5'-regulatory region of the vWF gene was associated with the highest plasma vWF levels in a normal population. To examine the association between -1185A/G polymorphism and coronary artery disease (CAD), 173 Brazilian Caucasian subjects submitted to coronary angiography were studied. Of these, 57 (33%) had normal coronary arteries (control group) and 116 (67%) had CAD (patient group). Plasma vWF levels were higher in patients (145 U/dl) than in controls (130 U/dl), but the differences were significant only for O blood group subjects. Polymerase chain reaction amplification of the 864-bp vWF promoter region followed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Von Willebrand factor; Coronary artery disease; DNA polymorphisms; Risk factor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600005

Focal adhesion kinase signaling in cardiac hypertrophy and failure
Provedor de dados: 56
Autores: Franchini,K.G.; Clemente,C.F.M.Z.; Marin,T.M.
Focal adhesion kinase (FAK) is a broadly expressed tyrosine kinase implicated in cellular functions such as migration, growth and survival. Emerging data support a role for FAK in cardiac development, reactive hypertrophy and failure. Data reviewed here indicate that FAK plays a critical role at the cellular level in the responses of cardiomyocytes and cardiac fibroblasts to biomechanical stress and to hypertrophic agonists such as angiotensin II and endothelin. The signaling mechanisms regulated by FAK are discussed to provide insight into its role in the pathophysiology of cardiac hypertrophy and failure.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Focal adhesion kinase; Mechanical signaling; Cardiovascular system; Signal transduction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100008

Inotropic effects of extracts of Psidium guajava L. (guava) leaves on the guinea pig atrium
Provedor de dados: 56
Autores: Conde Garcia,E.A.; Nascimento,V.T.; Santiago Santos,A.B.
Many pharmacological effects have been ascribed to extracts of Psidium guajava L. (guava) leaves. However, in spite of its widespread use in Brazilian folk medicine and a reasonable number of scientific reports about it, we could not find any study dealing with its action on the mammalian myocardium. In the present study, by measuring isometric force, we observed that the crude extract of P. guajava (water-alcohol extract obtained by macerating dry leaves) depresses the guinea pig atrial contractility in a concentration-dependent fashion (N = 8 hearts, 15 trials). The compound with cardiac activity was concentrated by extraction in a Soxhlet apparatus using 17 M glacial acetic acid after removing the less polar fractions (hexane, chloroform, acetone,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart contractility; Guinea pig; Myocardial inotropism; Psidium guajava L.; Folk medicine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500014

Increased expression and purification of soluble iron-regulatory protein 1 from Escherichia coli co-expressing chaperonins GroES and GroEL
Provedor de dados: 56
Autores: Carvalho,H.; Meneghini,R.
Iron is an essential metal for all living organisms. However, iron homeostasis needs to be tightly controlled since iron can mediate the production of reactive oxygen species, which can damage cell components and compromise the integrity and/or cause DNA mutations, ultimately leading to cancer. In eukaryotes, iron-regulatory protein 1 (IRP1) plays a central role in the control of intracellular iron homeostasis. This occurs by interaction of IRP1 with iron-responsive element regions at 5' of ferritin mRNA and 3' of transferrin mRNA which, respectively, represses translation and increases mRNA stability. We have expressed IRP1 using the plasmid pT7-His-hIRP1, which codifies for human IRP1 attached to an NH2-terminal 6-His tag. IRP1 was expressed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron metabolism; Iron-regulatory protein 1; Chaperonins; GroES; GroEL; Protein purification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400003

Oral tolerance induction with altered forms of ovalbumin
Provedor de dados: 56
Autores: Stransky,B.; Faria,A.M.C.; Vaz,N.M.
As a T cell-dependent phenomenon, oral tolerance is not expected to depend necessarily on native configuration of antigens. We investigated the induction of oral tolerance with modified ovalbumin (Ova). Oral administration of heat-denatured (HD-Ova) and cyanogen bromide-degraded ovalbumin was less effective than native Ova in inducing oral tolerance in B6D2F1 mice. HD-Ova was effective in suppressing delayed-type hypersensitivity (DTH) reactions but did not suppress specific antibody formation. Injection of Ova directly into the stomach, but not into the ileum or cecum, suppressed subsequent immunization to DTH reactions. Gavage with protease inhibitors (aprotinin or ovomucoid) before gavage with Ova was ineffective in blocking tolerance induction....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigen; Digestion; Oral tolerance; Mouse; Ovalbumin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300009

Effects of chronic heart disease on skeletal muscle fiber size
Provedor de dados: 56
Autores: Mattiello-Sverzut,A.C.; Chimelli,L.; Teixeira,S.; Pierre,M.; Oliveira,L.
Size changes in muscle fibers of subjects with chronic heart disease (CHD) have been reported, although a consensus has not been achieved. The aims of the present study were to investigate a possible association between CHD and fiber size changes in the brachial biceps compared to subjects without heart disease. Forty-six muscle samples were obtained in autopsies of individuals (13 to 84 years) without neuromuscular disorders, 19 (10 males and 9 females) with, and 27 (14 males and 13 females) without CHD. In all cases muscle sections were stained with hematoxylin and eosin and processed for the visualization of myofibrillar ATPase activity. The lesser diameter of type 1 and type 2 fibers was obtained tracing their outlines (at least 150 fibers of each type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphometry; Brachial biceps; Chronic heart disease; Post mortem muscle; Type 1 fiber hypertrophy; Type 2 fiber hypertrophy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200019

E3 ubiquitin ligase Cbl-b potentiates the apoptotic action of arsenic trioxide by inhibiting the PI3K/Akt pathway
Provedor de dados: 56
Autores: Yingchun,Li; Xiujuan,Qu; Jinglei,Qu; Ye,Zhang; Jing,Liu; Yuee,Teng; Xuejun,Hu; Kezuo,Hou; Yunpeng,Liu.
Arsenic trioxide (ATO) is a strong inducer of apoptosis in malignant hematological cells. Inducible phosphatidyl inositol 3 kinase (PI3K)-Akt activation promotes resistance to ATO. In the present study, we evaluated whether E3 ubiquitin ligase Cbl-b, a negative regulator of PI3K activation, is involved in the action of ATO. The effect of ATO on cell viability was measured by the Trypan blue exclusion assay or by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was determined by flow cytometry and protein expression was assayed by Western blotting. ATO decreased the viability of HL60 cells and induced cellular apoptosis, which was accompanied by transient activation of Akt. The PI3K/Akt inhibitor, LY294002,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arsenic trioxide; Apoptosis; Cbl-b; PI3K/Akt pathway.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200004

Effect of selective β-adrenoceptor blockade and surgical resection of the celiac-superior mesenteric ganglion complex on delayed liquid gastric emptying induced by dipyrone, 4-aminoantipyrine, and antipyrine in rats
Provedor de dados: 56
Autores: Vinagre,A.M.; Collares,E.F.
There is evidence for participation of peripheral β-adrenoceptors in delayed liquid gastric emptying (GE) induced in rats by dipyrone (Dp), 4-aminoantipyrine (AA), and antipyrine (At). The present study aimed to determine whether β-adrenoceptors are involved in delayed GE induced by phenylpyrazole derivatives and the role of the prevertebral sympathetic nervous system in this condition. Male Wistar rats weighing 220-280 g were used in the study. In the first experiment rats were intravenously pretreated with vehicle (V), atenolol 30 mg/kg (ATE, β1-adrenergic antagonist), or butoxamine 25 mg/kg (BUT, β2-adrenergic antagonist). In the second experiment, rats were pretreated with V or SR59230A 2 mg/kg (SRA, β3-adrenergic antagonist). In the third experiment,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dipyrone; 4-aminoantipyrine; Antipyrine; Β2-Adrenoceptor; Prevertebral sympathetic ganglia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300603

Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes
Provedor de dados: 56
Autores: Breder,J.S.C.; Pires,A.L.R.; Azevedo,F.F.; Apolinário,P.P.; Cantaruti,T.; Jiwani,S.I.; Moraes,Â.M.; Consonni,S.R.; Araújo,E.P.; Adams,G.G.; Saad,M.J.A.; Lima,M.H.M.
The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C57BL/6J mice; Wound healing; Wound dressing; Polysaccharides; Diabetes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100602

Reference values for lung function tests: I. Static volumes
Provedor de dados: 56
Autores: Neder,J.A.; Andreoni,S.; Castelo-Filho,A.; Nery,L.E.
Static lung volume (LV) measurements have a number of clinical and research applications; however, no previous studies have provided reference values for such tests using a healthy sample of the adult Brazilian population. With this as our main purpose, we prospectively evaluated 100 non-smoking subjects (50 males and 50 females), 20 to 80 years old, randomly selected from more than 8,000 individuals. Gender-specific linear prediction equations were developed by multiple regression analysis with total lung capacity (TLC), functional residual capacity (FRC), residual volume (RV), RV/TLC ratio and inspiratory capacity (IC) as dependent variables, and with age, height, weight, lean body mass and indexes of physical fitness as independent ones. Simpler...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung volumes; Pulmonary function tests; Pulmonary diseases.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600006

Silibinin improves palmitate-induced insulin resistance in C2C12 myotubes by attenuating IRS-1/PI3K/Akt pathway inhibition
Provedor de dados: 56
Autores: Li,H.B.; Yang,Y.R.Y.; Mo,Z.J.; Ding,Y.; Jiang,W.J.
The present study investigated the effect of silibinin, the principal potential anti-inflammatory flavonoid contained in silymarin, a mixture of flavonolignans extracted from Silybum marianum seeds, on palmitate-induced insulin resistance in C2C12 myotubes and its potential molecular mechanisms. Silibinin prevented the decrease of insulin-stimulated 2-NBDG (2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose) uptake and the downregulation of glutamate transporter type 4 (GLUT4) translocation in C2C12 myotubes induced by palmitate. Meanwhile, silibinin suppressed the palmitate-induced decrease of insulin-stimulated Akt Ser473 phosphorylation, which was reversed by wortmannin, a specific inhibitor of phosphatidylinositol-3-kinase (PI3K). We also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silibinin; Insulin resistance; Palmitate; C2C12.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500440

Gross proteinuria is a strong risk predictor for cardiovascular mortality in Brazilian type 2 diabetic patients
Provedor de dados: 56
Autores: Cardoso,C.R.L.; Salles,G.F.
Increased proteinuria is recognized as a risk predictor for all-cause and cardiovascular mortality in diabetic patients; however, no study has evaluated these relationships in Brazilian patients. The aim of this study was to investigate the prognostic value of gross proteinuria for all-cause and cardiovascular mortalities and for cardiovascular morbidity in a cohort study of 471 type 2 diabetic individuals followed for up to 7 years. Several clinical, laboratory and electrocardiographic variables were obtained at baseline. The relative risks for all-cause, cardiovascular and cardiac mortalities and for cardiovascular and cardiac events associated with the presence of overt proteinuria (&gt;0.5 g/24 h) were assessed by Kaplan-Meier survival curves and by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: All-cause mortality; Cardiovascular mortality; Cardiovascular morbidity; Proteinuria; Type 2 diabetes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800006

Effects of 174 G/C polymorphism in the promoter region of the interleukin-6 gene on plasma IL-6 levels and muscle strength in elderly women
Provedor de dados: 56
Autores: Pereira,D.S.; Garcia,D.M.; Narciso,F.M.S.; Santos,M.L.A.S.; Dias,J.M.D.; Queiroz,B.Z.; Souza,E.R.; Nóbrega,O.T.; Pereira,L.S.M.
We investigated the effect of -174 G/C single-nucleotide polymorphism in the promoter region of the IL6 gene on plasma IL-6 levels and muscle strength, and the relationship between IL-6 levels and muscle strength in elderly women. The sample consisted of 199 elderly residents (73.0 ± 7.8 years old) from rest homes and the community in Belo Horizonte, MG, Brazil. -174 G/C polymorphism was determined by direct sequencing of the product by PCR, and plasma IL-6 concentrations were measured by ELISA. Muscle strength in the knee joint was evaluated using a Biodex System 3 Pro® isokinetic dynamometer. ANCOVA was used to determine the effect of polymorphism on IL-6 levels and muscle strength, and the Pearson correlation coefficient to assess the relationship...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-6; Elderly; Muscular strength; 174 G/C polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200006

Regulation and function of neurogenesis in the adult vertebrate brain
Provedor de dados: 56
Autores: Mendez-Otero,R.; Zaverucha-do-Valle,C.; Gubert,F.; Freitas,G.R. de; Santiago,M.F.
Most adult tissues retain a reservoir of self-renewing, multipotent stem cells that can generate differentiated tissue components. Until recently, the brain was thought to be an exception to this rule and for many years the pervasive dogma of neurobiology relegated neurogenesis to the embryonic and earlier postnatal stages of development. The discovery of constant neuronal replacement in the adult brain has changed the way we think about neurological diseases and about the exploration of new strategies for brain repair. In this review we will explore the potential of adult neural stem cells and we will present some of our own work on this subject. We will also discuss the possibility that adult neurogenesis and neuronal replacement may also play a role in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neural stem cells; Adult neurogenesis; 9-O-acetyl GD3; Gangliosides.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000012

Ultrasound method applied to characterize healthy femoral diaphysis of Wistar rats in vivo
Provedor de dados: 56
Autores: Fontes-Pereira,A.; Matusin,D.P.; Rosa,P.; Schanaider,A.; von Krüger,M.A.; Pereira,W.C.A.
A simple experimental protocol applying a quantitative ultrasound (QUS) pulse-echo technique was used to measure the acoustic parameters of healthy femoral diaphyses of Wistar rats in vivo. Five quantitative parameters [apparent integrated backscatter (AIB), frequency slope of apparent backscatter (FSAB), time slope of apparent backscatter (TSAB), integrated reflection coefficient (IRC), and frequency slope of integrated reflection (FSIR)] were calculated using the echoes from cortical and trabecular bone in the femurs of 14 Wistar rats. Signal acquisition was performed three times in each rat, with the ultrasound signal acquired along the femur's central region from three positions 1 mm apart from each other. The parameters estimated for the three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone; Tissue characterization; Quantitative ultrasound; Clinical research protocols; Animal model.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500403

Specific alterations in plasma proteins during depressed, manic, and euthymic states of bipolar disorder
Provedor de dados: 56
Autores: Song,Y.R.; Wu,B.; Yang,Y.T.; Chen,J.; Zhang,L.J.; Zhang,Z.W.; Shi,H.Y.; Huang,C.L.; Pan,J.X.; Xie,P.
Bipolar disorder (BD) is a common psychiatric mood disorder affecting more than 1-2% of the general population of different European countries. Unfortunately, there is no objective laboratory-based test to aid BD diagnosis or monitor its progression, and little is known about the molecular basis of BD. Here, we performed a comparative proteomic study to identify differentially expressed plasma proteins in various BD mood states (depressed BD, manic BD, and euthymic BD) relative to healthy controls. A total of 10 euthymic BD, 20 depressed BD, 15 manic BD, and 20 demographically matched healthy control subjects were recruited. Seven high-abundance proteins were immunodepleted in plasma samples from the 4 experimental groups, which were then subjected to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bipolar disorder; Plasma; Proteomic; Carbonic anhydrase 1; Apolipoprotein.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100973

Effect of Ginkgo biloba on the reproductive outcome and oxidative stress biomarkers of streptozotocin-induced diabetic rats
Provedor de dados: 56
Autores: Rudge,M.V.C.; Damasceno,D.C.; Volpato,G.T.; Almeida,F.C.G.; Calderon,I.M.P.; Lemonica,I.P.
The aim of the present study was to evaluate the effect of Ginkgo biloba treatment (EGb 761, 200 mg kg-1 day-1) administered from day 0 to 20 of pregnancy on maternal reproductive performance and on the maternal and fetal liver antioxidant systems of streptozotocin-induced diabetic Wistar rats. On day 21 of pregnancy, the adult rats (weighing approximately 250 ± 50 g, minimum number = 13/group) were anesthetized to obtain maternal and fetal liver samples for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and total glutathione (GSH-t) determinations. The uterus was weighed with its contents. The diabetic (G3) and treated diabetic (G4) groups of rats presented significant maternal hyperglycemia, reduced term pregnancy rate,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ginkgo biloba; Diabetes mellitus; Pregnancy; Reproductive outcome; Oxidative stress.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800010

Monitoring renal function: measured and estimated glomerular filtration rates - a review
Provedor de dados: 56
Autores: Salgado,J.V.; Neves,F.A.; Bastos,M.G.; França,A.K.; Brito,D.J.; Santos,E.M.; Salgado Filho,N.
Chronic kidney disease (CKD) is a world-wide public health problem, with adverse outcomes of kidney failure, cardiovascular disease, and premature death. This finding has led to the hypothesis that earlier recognition of kidney disease and successful intervention may improve outcome. The National Kidney Foundation, through its Kidney Disease Outcomes Quality Initiative (K/DOQI), and other National institutions recommend glomerular filtration rate (GFR) for the definition, classification, screening, and monitoring of CKD. Blood creatinine clearance, the most widely used clinical marker of kidney function, is now recognized as an unreliable measure of GFR because serum creatinine is affected by age, weight, muscle mass, race, various medications, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney function; Glomerular filtration rate; Creatinine; Cystatin C; Measurement and calculation of GFR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600002

Reliability and validity of the interactive observation scale for psychiatric inpatients applied by nursing aides in daily ward practice
Provedor de dados: 56
Autores: Pedrão,L.J.; Hallak,J.E.C.; Bernardo,S.A.; Zuardi,A.W.
The main characteristic of the nursing Interactive Observation Scale for Psychiatric Inpatients (IOSPI) is the necessity of interaction between raters and patients during assessment. The aim of this study was to evaluate the reliability and validity of the scale in the "real" world of daily ward practice and to determine whether the IOSPI can increase the interaction time between raters and patients and influence the raters' opinion about mental illness. All inpatients of a general university hospital psychiatric ward were assessed daily over a period of two months by 9 nursing aides during the morning and afternoon shifts, with 273 pairs of daily observations. Once a week the patients were interviewed by a psychiatrist who filled in the Brief Psychiatric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nurse's rating scale; Reliability; Validity; Psychiatric inpatient.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700013

Essential oil of Lippia alba and its main constituent citral block the excitability of rat sciatic nerves
Provedor de dados: 56
Autores: Sousa,D.G.; Sousa,S.D.G.; Silva,R.E.R.; Silva-Alves,K.S.; Ferreira-da-Silva,F.W.; Kerntopf,M.R.; Menezes,I.R.A.; Leal-Cardoso,J.H.; Barbosa,R.
Lippia alba is empirically used for infusions, teas, macerates, and hydroalcoholic extracts because of its antispasmodic, analgesic, sedative, and anxiolytic effects. Citral is a mixture of trans-geranial and cis-neral and is the main constituent of L. alba essential oil and possesses analgesic, anxiolytic, anticonvulsant, and sedative effects. The present study evaluated the effects of the essential oil of L. alba (EOLa) and citral on compound action potentials (CAPs) in Wistar rat sciatic nerves. Both drugs inhibited CAP in a concentration-dependent manner. The calculated half-maximal inhibitory concentrations (IC50) of peak-to-peak amplitude were 53.2 µg/mL and 35.00 µg/mL (or 230 µM) for EOLa and citral, respectively. Peak-to-peak amplitude of the CAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citral; Nerve excitability; Lippia alba; Sciatic nerve; Essential oil; Compound action potential.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800697

Effects of acute administration of selective serotonin reuptake inhibitors on sympathetic nerve activity
Provedor de dados: 56
Autores: Tiradentes,R.V.; Pires,J.G.P.; Silva,N.F.; Ramage,A.G.; Santuzzi,C.H.; Futuro Neto,H.A.
Serotonergic mechanisms have an important function in the central control of circulation. Here, the acute effects of three selective serotonin (5-HT) reuptake inhibitors (SSRIs) on autonomic and cardiorespiratory variables were measured in rats. Although SSRIs require 2-3 weeks to achieve their full antidepressant effects, it has been shown that they cause an immediate inhibition of 5-HT reuptake. Seventy male Wistar rats were anesthetized with urethane and instrumented to record blood pressure, heart rate, renal sympathetic nerve activity (RSNA), and respiratory frequency. At lower doses, the acute cardiovascular effects of fluoxetine, paroxetine and sertraline administered intravenously were insignificant and variable. At middle and higher doses, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoxetine; Paroxetine; Renal nerve activity; Serotonin; Sertraline; Sympathetic activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700554

Exercise improved lipid metabolism and insulin sensitivity in rats fed a high-fat diet by regulating glucose transporter 4 (GLUT4) and musclin expression
Provedor de dados: 56
Autores: Yu,J.; Zheng,J.; Liu,X.F.; Feng,Z.L.; Zhang,X.P.; Cao,L.L.; Zhou,Z.P.
This study aimed to evaluate the effects of exercise training on triglyceride deposition and the expression of musclin and glucose transporter 4 (GLUT4) in a rat model of insulin resistance. Thirty male Sprague-Dawley rats (8 weeks old, weight 160±10 g) were fed a high-fat diet (40% calories from fat) and randomly divided into high-fat control group and swimming intervention group. Rats fed with standard food served as normal control. We found that 8-week swimming intervention significantly decreased body weight (from 516.23±46.27 to 455.43±32.55 g) and visceral fat content (from 39.36±2.50 to 33.02±2.24 g) but increased insulin sensitivity index of the rats fed with a high-fat diet. Moreover, swimming intervention improved serum levels of TG (from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin resistance; Musclin; GLUT4; Swimming; Triglyceride deposition.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500604

Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats
Provedor de dados: 56
Autores: Aguiar,C.E.; Cadore,L.P.; Padoin,M.J.; Barbosa-Coutinho,L.M.; Lucion,A.B.
Immunohistochemistry was used to evaluate the effects of neonatal handling and aversive stimulation during the first 10 days of life on the number of corticotrophs in the anterior lobe of the pituitary of 11-day-old male Wistar rats. Since adult rats handled during infancy respond with reduced corticosterone secretion in response to stressors and with less behavior inhibition in novel environments, we assumed that neonatal stimulation could affect pituitary morphology during this critical period of cell differentiation. Three groups of animals were studied: intact (no manipulation, N = 5), handled (N = 5) and stimulated (submitted to 3 different aversive stimuli, N = 5). The percentage of ACTH-immunoreactive cells in the anterior lobe of the pituitary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACTH; Immunohistochemistry; Pituitary; Neonatal handling; Stress.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200013

Poor sleep in middle-aged women is not associated with menopause per se
Provedor de dados: 56
Autores: Tao,M.F.; Sun,D.M.; Shao,H.F.; Li,C.B.; Teng,Y.C.
Whether sleep problems of menopausal women are associated with vasomotor symptoms and/or changes in estrogen levels associated with menopause or age-related changes in sleep architecture is unclear. This study aimed to determine if poor sleep in middle-aged women is correlated with menopause. This study recruited women seeking care for the first time at the menopause outpatient department of our hospital. Inclusion criteria were an age ≥40 years, not taking any medications for menopausal symptoms, and no sleeping problems or depression. Patients were assessed with the Pittsburgh Sleep Quality Index (PSQI), modified Kupperman Index (KI), and Menopause Rating Scale (MRS). A PSQI score of &lt;7 indicated no sleep disorder and ≥7 indicated a sleep disorder....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kupperman Index; Menopause; Menopause Rating Scale; Pittsburgh Sleep Quality Index; Sleep disorder; Quality of life.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100701

Glomerular damage as a predictor of renal allograft loss
Provedor de dados: 56
Autores: Moscoso-Solorzano,G.; Câmara,N.O.S.; Franco,M.F.; Araújo,S.; Ortega,F.; Pacheco-Silva,A.; Mastroianni-Kirsztajn,G.
Interstitial fibrosis and tubular atrophy (IF/TA) are the most common cause of renal graft failure. Chronic transplant glomerulopathy (CTG) is present in approximately 1.5-3.0% of all renal grafts. We retrospectively studied the contribution of CTG and recurrent post-transplant glomerulopathies (RGN) to graft loss. We analyzed 123 patients with chronic renal allograft dysfunction and divided them into three groups: CTG (N = 37), RGN (N = 21), and IF/TA (N = 65). Demographic data were analyzed and the variables related to graft function identified by statistical methods. CTG had a significantly lower allograft survival than IF/TA. In a multivariate analysis, protective factors for allograft outcomes were: use of angiotensin-converting enzyme inhibitor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney transplantation; Chronic allograft nephropathy; Glomerulonephritis; Transplant glomerulopathy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600006

Abnormalities of hippocampal signal intensity in patients with familial mesial temporal lobe epilepsy
Provedor de dados: 56
Autores: Coan,A.C.; Kobayashi,E.; Lopes-Cendes,I.; Li,L.M.; Cendes,F.
Mesial temporal lobe epilepsy (MTLE) is associated with hippocampal atrophy and hippocampal signal abnormalities. In our series of familial MTLE (FMTLE), we found a high proportion of hippocampal abnormalities. To quantify signal abnormalities in patients with FMTLE we studied 152 individuals (46 of them asymptomatic) with FMTLE. We used NIH-Image® for volumetry and signal quantification in coronal T1 inversion recovery and T2 for all cross-sections of the hippocampus. Values diverging by 2 or more SD from the control mean were considered abnormal. T2 hippocampal signal abnormalities were found in 52% of all individuals: 54% of affected subjects and 48% of asymptomatic subjects. T1 hippocampal signal changes were found in 34% of all individuals: 42.5% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hippocampal signal abnormalities; Familial mesial temporal lobe epilepsy; Hippocampal volumetry; Magnetic resonance imaging.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600007

Axonal pathfinding mechanisms at the cortical midline and in the development of the corpus callosum
Provedor de dados: 56
Autores: Richards,L.J.
The corpus callosum is a large fiber tract that connects neurons in the right and left cerebral hemispheres. Agenesis of the corpus callosum (ACC) is associated with a large number of human syndromes but little is known about why ACC occurs. In most cases of ACC, callosal axons are able to grow toward the midline but are unable to cross it, continuing to grow into large swirls of axons known as Probst bundles. This phenotype suggests that in some cases ACC may be due to defects in axonal guidance at the midline. General guidance mechanisms that influence the development of axons include chemoattraction and chemorepulsion, presented by either membrane-bound or diffusible molecules. These molecules are not only expressed by the final target but by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Midline glia; Glial wedge; Glial sling; Indusium griseum glia; Perforating pathway; Corpus callosum.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001200004

Diverse effects of renal denervation on ventricular hypertrophy and blood pressure in DOCA-salt hypertensive rats
Provedor de dados: 56
Autores: Cabral,A.M.; Silva,I.F.; Gardioli,C.R.; Mauad,H.; Vasquez,E.C.
Cardiac hypertrophy that accompanies hypertension seems to be a phenomenon of multifactorial origin whose development does not seem to depend on an increased pressure load alone, but also on local growth factors and cardioadrenergic activity. The aim of the present study was to determine if sympathetic renal denervation and its effects on arterial pressure level can prevent cardiac hypertrophy and if it can also delay the onset and attenuate the severity of deoxycorticosterone acetate (DOCA)-salt hypertension. DOCA-salt treatment was initiated in rats seven days after uninephrectomy and contralateral renal denervation or sham renal denervation. DOCA (15 mg/kg, sc) or vehicle (soybean oil, 0.25 ml per animal) was administered twice a week for two weeks....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ventricular hypertrophy; Renal denervation; DOCA-salt hypertension; Blood pressure.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400018

Recurrent hepatitis C treatment with direct acting antivirals – a real life study at a Brazilian liver transplant center
Provedor de dados: 56
Autores: Zanaga,L.P.; Santos,A.G.; Ataíde,E.C.; Boin,I.F.S.F.; Stucchi,R.S.B.
Recurrent hepatitis C (HCV) after liver transplantation (LT) is an important cause of morbidity and mortality. Antiviral treatment is recommended to avoid unfavorable outcomes. Direct-acting antivirals (DAA) have transformed HCV treatment, with higher efficacy and fewer side-effects than interferon-based therapies traditionally used. To evaluate DAA treatment outcomes at a Brazilian transplant unit, data of patients who finished HCV treatment at the Liver Transplant Unit of the University of Campinas were analyzed. Treatment consisted of sofosbuvir, daclatasvir, and ribavirin, for 12 or 24 weeks, according to the national guidelines. Fifty-five patients completed antiviral treatment and 54 had HCV-viral load results available. The majority of patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Liver transplantation; Direct acting antivirals; Sofosbuvir; Daclatasvir; Recurrent hepatitis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800606

Paradoxical sleep deprivation increases plasma endothelin levels
Provedor de dados: 56
Autores: Palma,B.D.; Gabriel Jr.,A.; Bignotto,M.; Tufik,S.
The endothelins (ET-1, 2 and 3) constitute a family of 21 amino acid peptides with potent biological activities. ET-1 is one of the most potent endogenous vasoconstrictors so far identified and its increased concentration in plasma appears to be closely related to the pathogenesis of arterial hypertension as well as to obstructive sleep apnea (OSA). OSA patients exhibit repetitive episodes of apnea and hypopnea that result in hypoxia and consecutive arousals. These patients are chronically sleep deprived, which may aggravate the hypertensive features, since literature data show that sleep deprivation results in hypertension both in humans and in animals. Based on the reported relationship between ET-1, hypertension and sleep deprivation consequences, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Endothelin; Sleep deprivation; Rat; Hypertension.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100011

Pressure-assisted cold denaturation of hen egg white lysozyme: the influence of co-solvents probed by hydrogen exchange nuclear magnetic resonance
Provedor de dados: 56
Autores: Vogtt,K.; Winter,R.
COSY proton nuclear magnetic resonance was used to measure the exchange rates of amide protons of hen egg white lysozyme (HEWL) in the pressure-assisted cold-denatured state and in the heat-denatured state. After dissolving lysozyme in deuterium oxide buffer, labile protons exchange for deuterons in such a way that exposed protons are substituted rapidly, whereas "protected" protons within structured parts of the protein are substituted slowly. The exchange rates k obs were determined for HEWL under heat treatment (80ºC) and under high pressure conditions at low temperature (3.75 kbar, -13ºC). Moreover, the influence of co-solvents (sorbitol, urea) on the exchange rate was examined under pressure-assisted cold denaturation conditions, and the corresponding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pressure-assisted cold denaturation; Co-solvents; Lysozyme; Hydrogen exchange nuclear magnetic resonance.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800005

The non-palindromic adaptor-PCR method for the identification of the T-cell receptor genes of an interferon-gamma-secreting T-cell hybridomaspecific for trans-sialidase, an immunodominant Trypanosoma cruzi antigen
Provedor de dados: 56
Autores: Hiyane,M.I.; Boscardin,S.B.; Rodrigues,M.M.
Cloning of the T-cell receptor genes is a critical step when generating T-cell receptor transgenic mice. Because T-cell receptor molecules are clonotypical, isolation of their genes requires reverse transcriptase-assisted PCR using primers specific for each different Valpha or Vß genes or by the screening of cDNA libraries generated from RNA obtained from each individual T-cell clone. Although feasible, these approaches are laborious and costly. The aim of the present study was to test the application of the non-palindromic adaptor-PCR method as an alternative to isolate the genes encoding the T-cell receptor of an antigen-specific T-cell hybridoma. For this purpose, we established hybridomas specific for trans-sialidase, an immunodominant Trypanosoma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NPA-PCR method; T-cell receptor; Trypanosoma cruzi; Trans-sialidase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300005

Effect of age and gender on sweat lactate and ammonia concentrations during exercise in the heat
Provedor de dados: 56
Autores: Meyer,F.; Laitano,O.; Bar-Or,O.; McDougall,D.; Heigenhauser,G.J.F.
The dependence of sweat composition and acidity on sweating rate (SR) suggests that the lower SR in children compared to adults may be accompanied by a higher level of sweat lactate (Lac-) and ammonia (NH3) and a lower sweat pH. Four groups (15 girls, 18 boys, 8 women, 8 men) cycled in the heat (42ºC, 20% relative humidity) at 50% VO2max for two 20-min bouts with a 10-min rest before bout 1 and between bouts. Sweat was collected into plastic bags attached to the subject's lower back. During bout 1, sweat from girls and boys had higher Lac- concentrations (23.6 ± 1.2 and 21.2 ± 1.7 mM; P &lt; 0.05) than sweat from women and men (18.2 ± 1.9 and 14.8 ± 1.6 mM, respectively), but Lac- was weakly associated with SR (P &gt; 0.05; r = -0.27). Sweat Lac-...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sweat pH; Electrolytes; Ammonia; Lactate.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100017

High prevalence of methicillin resistance and PVL genes among Staphylococcus aureus isolates from the nares and skin lesions of pediatric patients with atopic dermatitis
Provedor de dados: 56
Autores: Cavalcante,F.S.; Abad,E.D.; Lyra,Y.C.; Saintive,S.B.; Ribeiro,M.; Ferreira,D.C.; Santos,K.R.N. dos.
Staphylococcus aureus is highly prevalent among patients with atopic dermatitis (AD), and this pathogen may trigger and aggravate AD lesions. The aim of this study was to determine the prevalence of S. aureus in the nares of pediatric subjects and verify the phenotypic and molecular characteristics of the isolates in pediatric patients with AD. Isolates were tested for antimicrobial susceptibility, SCCmec typing, and Panton-Valentine Leukocidin (PVL) genes. Lineages were determined by pulsed-field gel electrophoresis and multilocus sequence typing (MLST). AD severity was assessed with the Scoring Atopic Dermatitis (SCORAD) index. Among 106 patients, 90 (85%) presented S. aureus isolates in their nares, and 8 also presented the pathogen in their skin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atopic dermatitis; Staphylococcus aureus; Nasal colonization; Skin lesions; SCORAD.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700588

Ectopic development of skeletal muscle induced by subcutaneous transplant of rat satellite cells
Provedor de dados: 56
Autores: Fukushima,M.G.; Furlan,I.; Chiavegatti,T.; Kiyomoto,B.H.; Godinho,R.O.
The present study analyzes the ectopic development of the rat skeletal muscle originated from transplanted satellite cells. Satellite cells (10(6) cells) obtained from hindlimb muscles of newborn female 2BAW Wistar rats were injected subcutaneously into the dorsal area of adult male rats. After 3, 7, and 14 days, the transplanted tissues (N = 4-5) were processed for histochemical analysis of peripheral nerves, inactive X-chromosome and acetylcholinesterase. Nicotinic acetylcholine receptors (nAChRs) were also labeled with tetramethylrhodamine-labeled alpha-bungarotoxin. The development of ectopic muscles was successful in 86% of the implantation sites. By day 3, the transplanted cells were organized as multinucleated fibers containing multiple clusters of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skeletal muscle; Satellite cells; Stem cells; Transplantation; Myogenesis; 2BAW rats.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300007

Leukocytosis, muscle damage and increased lymphocyte proliferative response after an adventure sprint race
Provedor de dados: 56
Autores: Tossige-Gomes,R.; Ottone,V.O.; Oliveira,P.N.; Viana,D.J.S.; Araújo,T.L.; Gripp,F.J.; Rocha-Vieira,E.
The effect of an adventure sprint race (ASR) on T-cell proliferation, leukocyte count and muscle damage was evaluated. Seven young male runners completed an ASR in the region of Serra do Espinhaço, Brazil. The race induced a strong leukocytosis (6.22±2.04×103 cells/mm3 beforevs 14.81±3.53×103 cells/mm3after the race), marked by a significant increase of neutrophils and monocytes (P&lt;0.05), but not total lymphocytes, CD3+CD4+ or CD3+CD8+ cells. However, the T-cell proliferative response to mitogenic stimulation was increased (P=0.025) after the race, which contradicted our hypothesis that ASR, as a high-demand competition, would inhibit T-cell proliferation. A positive correlation (P=0.03, r=0.79) was observed between the proliferative response of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strenuous exercise; Lymphocyte; Proliferation; Aspartate amino transferase; Immunosuppression.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600492

Cardiorespiratory fitness, pulmonary function and C-reactive protein levels in nonsmoking individuals with diabetes
Provedor de dados: 56
Autores: Francisco,C.O.; Catai,A.M.; Moura-Tonello,S.C.G.; Lopes,S.L.B.; Benze,B.G.; Del Vale,A.M.; Leal,A.M.O.
The objective of this study was to evaluate cardiorespiratory fitness and pulmonary function and the relationship with metabolic variables and C-reactive protein (CRP) plasma levels in individuals with diabetes mellitus (DM). Nineteen men with diabetes and 19 age- and gender-matched control subjects were studied. All individuals were given incremental cardiopulmonary exercise and pulmonary function tests. In the exercise test, maximal workload (158.3±22.3vs 135.1±25.2, P=0.005), peak heart rate (HRpeak: 149±12 vs 139±10, P=0.009), peak oxygen uptake (VO2peak: 24.2±3.2 vs18.9±2.8, P&lt;0.001), and anaerobic threshold (VO2VT: 14.1±3.4 vs 12.2±2.2, P=0.04) were significantly lower in individuals with diabetes than in control subjects. Pulmonary function test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Pulmonary function test; Cardiopulmonary exercise test; Glycemic control; C-reactive protein.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500426

Genetic alterations in head and neck squamous cell carcinomas
Provedor de dados: 56
Autores: Nagai,M.A.
The genetic alterations observed in head and neck cancer are mainly due to oncogene activation (gain of function mutations) and tumor suppressor gene inactivation (loss of function mutations), leading to deregulation of cell proliferation and death. These genetic alterations include gene amplification and overexpression of oncogenes such as myc, erbB-2, EGFR and cyclinD1 and mutations, deletions and hypermethylation leading to p16 and TP53 tumor suppressor gene inactivation. In addition, loss of heterozygosity in several chromosomal regions is frequently observed, suggesting that other tumor suppressor genes not yet identified could be involved in the tumorigenic process of head and neck cancers. The exact temporal sequence of the genetic alterations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic alterations; Oncogene; Tumor suppressor gene; Head and neck cancer.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700015

Collagen arrangement in hepatic granuloma in mice infected with Schistosoma mansoni: dependence on fiber radiation centers
Provedor de dados: 56
Autores: Lenzi,H.L.; Kimmel,E.; Schechtman,H.; Pelajo-Machado,M.; Vale,B.S.; Panasco,M.S.; Lenzi,J.A.
The collagen structure of isolated and in situ liver granuloma from Swiss Webster mice infected with Schistosoma mansoni was sequentially and three-dimensionally analyzed during different times of infection (early acute, acute, transitional acute-chronic, and chronic phases) by laser scanning confocal microscopy and electron scanning variable vacuum microscopy. The initial granuloma structure is characterized by vascular collagen residues and by anchorage points (or fiber radiation centers), from where collagenous fibers are angularly shed and self-assembled. During the exudative-productive stage, the self-assembly of these fibers minimizes energy and mass through continuous tension and focal compression. The curvature or angles between collagen fibers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Granuloma; Schistosoma mansoni; Collagen; Extracellular matrix; Confocal microscopy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500018

Transcriptomic differential lncRNA expression is involved in neuropathic pain in rat dorsal root ganglion after spared sciatic nerve injury
Provedor de dados: 56
Autores: Mao,P.; Li,C.R.; Zhang,S.Z.; Zhang,Y.; Liu,B.T.; Fan,B.F.
Dorsal root ganglia (DRG) neurons regenerate spontaneously after traumatic or surgical injury. Long noncoding RNAs (lncRNAs) are involved in various biological regulation processes. Conditions of lncRNAs in DRG neuron injury deserve to be further investigated. Transcriptomic analysis was performed by high-throughput Illumina HiSeq2500 sequencing to profile the differential genes in L4–L6 DRGs following rat sciatic nerve tying. A total of 1,228 genes were up-regulated and 1,415 down-regulated. By comparing to rat lncRNA database, 86 known and 26 novel lncRNA genes were found to be differential. The 86 known lncRNA genes modulated 866 target genes subject to gene ontology (GO) and KEGG enrichment analysis. The genes involved in the neurotransmitter status of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spared nerve injury; Neuropathic pain; Long noncoding RNA; Transcriptome; Hypm gene.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000601

A protein with amino acid sequence homology to bovine insulin is present in the legume Vigna unguiculata (cowpea)
Provedor de dados: 56
Autores: Venâncio,T.M.; Oliveira,A.E.A.; Silva,L.B.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.
Since the discovery of bovine insulin in plants, much effort has been devoted to the characterization of these proteins and elucidation of their functions. We report here the isolation of a protein with similar molecular mass and same amino acid sequence to bovine insulin from developing fruits of cowpea (Vigna unguiculata) genotype Epace 10. Insulin was measured by ELISA using an anti-human insulin antibody and was detected both in empty pods and seed coats but not in the embryo. The highest concentrations (about 0.5 ng/µg of protein) of the protein were detected in seed coats at 16 and 18 days after pollination, and the values were 1.6 to 4.0 times higher than those found for isolated pods tested on any day. N-terminal amino acid sequencing of insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Insulin-like; Cowpea; Vigna unguiculata; Developing fruits; Sequence homology; Evolution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900004

Proliferation of differentiated glial cells in the brain stem
Provedor de dados: 56
Autores: Barradas,P.C.; Cavalcante,L.A.
Classical studies of macroglial proliferation in muride rodents have provided conflicting evidence concerning the proliferating capabilities of oligodendrocytes and microglia. Furthermore, little information has been obtained in other mammalian orders and very little is known about glial cell proliferation and differentiation in the subclass Metatheria although valuable knowledge may be obtained from the protracted period of central nervous system maturation in these forms. Thus, we have studied the proliferative capacity of phenotypically identified brain stem oligodendrocytes by tritiated thymidine radioautography and have compared it with known features of oligodendroglial differentiation as well as with proliferation of microglia in the opossum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lsqb; 3Hrsqb; -thymidine radioautography oligodendroglial proliferation 2'3'-cyclic nucleotide 3'-phosphodiesterase microglial proliferation lectin marsupial.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200009

Mental rotation of anthropoid hands: a chronometric study
Provedor de dados: 56
Autores: Gawryszewski,L.G.; Silva-dos-Santos,C.F.; Santos-Silva,J.C.; Lameira,A.P.; Pereira Jr,A.
It has been shown that mental rotation of objects and human body parts is processed differently in the human brain. But what about body parts belonging to other primates? Does our brain process this information like any other object or does it instead maximize the structural similarities with our homologous body parts? We tried to answer this question by measuring the manual reaction time (MRT) of human participants discriminating the handedness of drawings representing the hands of four anthropoid primates (orangutan, chimpanzee, gorilla, and human). Twenty-four right-handed volunteers (13 males and 11 females) were instructed to judge the handedness of a hand drawing in palm view by pressing a left/right key. The orientation of hand drawings varied from...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Handedness recognition; Mental rotation; Manual reaction time; Motor imagery; Mirror neurons; Imitation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300013

A study of the interaction between Helicobacter pylori and components of the human fibrinolytic system
Provedor de dados: 56
Autores: Yarzábal,A.; Avilán,L.; Hoelzl,K.; Muñoz,M. de; Puig,J.; Kansau,I.
The interaction of plasminogen, tissue plasminogen activator (t-PA) and urokinase with a clinical strain of Helicobacter pylori was studied. Plasminogen bound to the surface of H. pylori cells in a concentration-dependent manner and could be activated to the enzymatic form, plasmin, by t-PA. Affinity chromatography assays revealed a plasminogen-binding protein of 58.9 kDa in water extracts of surface proteins. Surface-associated plasmin activity, detected with the chromogenic substrate CBS 00.65, was observed only when plasminogen and an exogenous activator were added to the cell suspension. The two physiologic plasminogen activators, t-PA and urokinase, were also shown to bind to and remain active on the surface of bacterial cells. epsilon-Aminocaproic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Plasminogen; T-PA; Urokinase; Plasminogen activation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900004

Bioelectrical impedance spectroscopy for the assessment of body fluid volumes of term neonates
Provedor de dados: 56
Autores: Ferreira,D.M.; Souza,M.N.
The assessment of fluid volume in neonates by a noninvasive, inexpensive, and fast method can contribute significantly to increase the quality of neonatal care. The objective of the present study was to calibrate an acquisition system and software to estimate the bioelectrical impedance parameters obtained by a method of bioelectrical impedance spectroscopy based on step response and to develop specific equations for the neonatal population to determine body fluid compartments. Bioelectric impedance measurements were performed by a laboratory homemade instrument. The volumes were estimated in a clinical study on 30 full-term neonates at four different times during the first month of life. During the first 24 hours of life the total body water,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioelectrical impedance spectroscopy; Body water; Extracellular water; Intracellular water; Neonatal handling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100002

Increased expression of p38 mitogen- activated protein kinase is related to the acute renal lesions induced by gentamicin
Provedor de dados: 56
Autores: Volpini,R.A.; Balbi,A.P.C.; Costa,R.S.; Coimbra,T.M.
Mitogen-activated protein kinases (MAPK) may be involved in the pathogenesis of acute renal failure. This study investigated the expression of p-p38 MAPK and nuclear factor kappa B (NF-kappaB) in the renal cortex of rats treated with gentamicin. Twenty rats were injected with gentamicin, 40 mg/kg, im, twice a day for 9 days, 20 with gentamicin + pyrrolidine dithiocarbamate (PDTC, an NF-kappaB inhibitor), 14 with 0.15 M NaCl, im, twice a day for 9 days, and 14 with 0.15 M NaCl , im, twice a day for 9 days and PDTC, 50 mg kg-1 day-1, ip, twice a day for 15 days. The animals were killed 5 and 30 days after the last of the injections and the kidneys were removed for histological, immunohistochemical and Western blot analysis and for nitrate determination. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: P38 mitogen-activated protein kinase; Nuclear factor-kappaB; Inflammation; Renal fibrosis; Gentamicin-induced acute tubular necrosis; Nitric oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600016

Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice
Provedor de dados: 56
Autores: Song,Jia; Wang,Xue; Huang,Yu; Qu,Yidi; Zhang,Guirong; Wang,Di.
Marasmius androsaceus is a medicinal fungus mainly used to treat various forms of pain in China. This study investigated the analgesic effects of an ethanol extract of M. androsaceus (MAE) and its potential molecular mechanisms. Oral administration of MAE (50, 200, and 1000 mg/kg) had significant analgesic effects in an acid-induced writhing test, a formalin test, and a hot-plate test, with effectiveness similar to tramadol (the positive control drug). The autonomic activity test showed that MAE had no harmful effects on the central nervous system in mice. MAE resulted in significantly enhanced levels of noradrenalin and 5-hydroxytryptamine in serum but suppressed both of these neurotransmitters in the hypothalamus after 30 s of hot-plate stimulation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marasmius androsaceus; Analgesic ability; Monoamine neurotransmitters; Calcium channels.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400613

Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiae
Provedor de dados: 56
Autores: Pádula,M.; Boiteux,S.
In the present study, we analyzed DNA damage induced by phycocyanin (PHY) in the presence of visible light (VL) using a set of repair endonucleases purified from Escherichia coli. We demonstrated that the profile of DNA damage induced by PHY is clearly different from that induced by molecules that exert deleterious effects on DNA involving solely singlet oxygen as reactive species. Most of PHY-induced lesions are single strand breaks and, to a lesser extent, base oxidized sites, which are recognized by Nth, Nfo and Fpg enzymes. High pressure liquid chromatography coupled to electrochemical detection revealed that PHY photosensitization did not induce 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) at detectable levels. DNA repair after PHY...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phycocyanin; Saccharomyces cerevisiae; OGG1; Nucleotide excision repair.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900002

Can calories from ethanol contribute to body weight preservation by malnourished rats?
Provedor de dados: 56
Autores: Aguiar,A.S.; Da-Silva,V.A.; Boaventura,G.T.
Our objective was to compare the use of calories from ethanol by well-nourished and malnourished rats in terms of body weight. Female Wistar rats weighing 170-180 g at the beginning of the study were used. The animals were divided into two groups (N = 12 each): group W received water ad libitum and group E an ethanol solution ad libitum as the only source of liquid throughout the experiment. The concentration of ethanol was increased weekly from 0 to 5, 10, 20 and 40% (v/v). In the well-nourished phase (A), all rats received food ad libitum (AW and AE). Ethanol treatment (AE) was then interrupted and water was offered to both groups. After 2 weeks both AW and AE rats were submitted to food restriction (50% of group AW food consumption), thus initiating the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ethanol; Weight gain; Malnutrition; Rats; Toxicity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600009

Cardiac and pulmonary alterations in symptomatic and asymptomatic dogs infectednaturally with Leishmania (Leishmania) chagasi
Provedor de dados: 56
Autores: Alves,G.B.B.; Pinho,F.A.; Silva,S.M.M.S.; Cruz,M.S.P.; Costa,F.A.L.
Fifteen symptomatic and seven asymptomatic dogs infected naturally with Leishmania chagasi were examined in order to identify the presence of parasites and changes in heart and lung. Histopathological, cytological, and immunohistochemical analyses were performed on samples of heart and lung tissues. An inflammatory reaction characterized by inflammatory mononuclear, perivascular and intermuscular infiltrates was observed in both symptomatic and asymptomatic animals on histopathological analysis of the heart. In the lung, there was thickening of the alveolar septa due to congestion, edema, inflammatory infiltrate, and fibroblast proliferation. A focal reaction was observed although a diffuse reaction was present in both groups. On cytological examination,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Dog; Lungs; Heart.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300014

Linear competitive inhibition of human tissue kallikrein by 4-aminobenzamidine and benzamidine and linear mixed inhibition by 4-nitroaniline and aniline
Provedor de dados: 56
Autores: Sousa,M.O.; Miranda,T.L.S.; Costa,E.B.; Bittar,E.R.; Santoro,M.M.; Figueiredo,A.F.S.
Hydrolysis of D-valyl-L-leucyl-L-arginine p-nitroanilide (7.5-90.0 µM) by human tissue kallikrein (hK1) (4.58-5.27 nM) at pH 9.0 and 37ºC was studied in the absence and in the presence of increasing concentrations of 4-aminobenzamidine (96-576 µM), benzamidine (1.27-7.62 mM), 4-nitroaniline (16.5-66 µM) and aniline (20-50 mM). The kinetic parameters determined in the absence of inhibitors were: Km = 12.0 ± 0.8 µM and k cat = 48.4 ± 1.0 min-1. The data indicate that the inhibition of hK1 by 4-aminobenzamidine and benzamidine is linear competitive, while the inhibition by 4-nitroaniline and aniline is linear mixed, with the inhibitor being able to bind both to the free enzyme with a dissociation constant Ki yielding an EI complex, and to the ES complex with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinetics of human tissue kallikrein inhibition; Tissue kallikrein; 4-nitroaniline; Aniline; Benzamidine; 4-aminobenzamidine; Enzyme inhibition.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100004

Effect of dimethylsulfoxide on sphingomyelinase activity and cholesterol metabolism in Niemann-Pick type C fibroblasts
Provedor de dados: 56
Autores: Scalco,F.B.; Giugliani,R.; Tobo,P.; Coelho,J.C.
Niemann-Pick type C (NPC) fibroblasts present a large concentration of cholesterol in their cytoplasm due to a still unidentified deficiency in cholesterol metabolism. The influence of dimethylsulfoxide (DMSO) on the amount of intracellular cholesterol was measured in 8 cultures of normal fibroblasts and in 7 fibroblast cultures from NPC patients. DMSO was added to the fibroblast cultures at three different concentrations (1, 2 and 4%, v/v) and the cultures were incubated for 24 h. Sphingomyelinase activity was significantly increased in both groups of cells only when incubated with 2% DMSO (59.4 ± 9.1 and 77.0 ± 9.1 nmol h-1 mg protein-1, controls without and with 2% DMSO, respectively; 47.7 ± 5.2 and 55.8 ± 4.1 nmol h-1 mg protein-1, NPC without and with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterol metabolism; Sphingomyelinase; Niemann-Pick disease; Lysosomal storage disorders.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100003

Autonomic control of cardiorespiratory interactions in fish, amphibians and reptiles
Provedor de dados: 56
Autores: Taylor,E.W.; Leite,C.A.C.; Skovgaard,N.
Control of the heart rate and cardiorespiratory interactions (CRI) is predominantly parasympathetic in all jawed vertebrates, with the sympathetic nervous system having some influence in tetrapods. Respiratory sinus arrhythmia (RSA) has been described as a solely mammalian phenomenon but respiration-related beat-to-beat control of the heart has been described in fish and reptiles. Though they are both important, the relative roles of feed-forward central control and peripheral reflexes in generating CRI vary between groups of fishes and probably between other vertebrates. CRI may relate to two locations for the vagal preganglionic neurons (VPN) and in particular cardiac VPN in the brainstem. This has been described in representatives from all vertebrate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiorespiratory interactions; Autonomic nervous system; Cardiac vagal preganglionic neurons; Central interactions; Peripheral reflex control; Non-adrenergic-non-cholinergic (NANC) control of heart rate.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700001

Protein-mediated surface structuring in biomembranes
Provedor de dados: 56
Autores: Maggio,B.; Rosetti,C.M.; Borioli,G.A.; Fanani,M.L.; Del Boca,M.
The lipids and proteins of biomembranes exhibit highly dissimilar conformations, geometrical shapes, amphipathicity, and thermodynamic properties which constrain their two-dimensional molecular packing, electrostatics, and interaction preferences. This causes inevitable development of large local tensions that frequently relax into phase or compositional immiscibility along lateral and transverse planes of the membrane. On the other hand, these effects constitute the very codes that mediate molecular and structural changes determining and controlling the possibilities for enzymatic activity, apposition and recombination in biomembranes. The presence of proteins constitutes a major perturbing factor for the membrane sculpturing both in terms of its surface...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid monolayers; Sphingomyelinase; C-Fos; Segregated lipid domains; Lipid-protein interaction; Anphitropic proteins.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200002

Gastrodin induced HO-1 and Nrf2 up-regulation to alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells through p38 MAPK phosphorylation
Provedor de dados: 56
Autores: Zhang,Hongbin; Yuan,Bo; Huang,Hanfei; Qu,Siming; Yang,Shikun; Zeng,Zhong.
Nuclear factor erythroid-related factor 2 (Nrf2) has been implicated in several detoxifying and antioxidant defense processes. Nrf2-mediated heme oxygenase-1 (HO-1) expression was demonstrated to play a key role against oxidative stress. Gastrodin (GSTD) is a well-known active compound isolated from the roots of Rhizoma gastrodiae, a plant used in ancient Chinese traditional medicine. The aim of this work was to investigate whether GSTD could alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells (LSECs). In LSECs exposed to 1 mM H2O2, treatment with GSTD (1, 10, or 50 µM) resulted in higher cell viability than the untreated control. Treated cells maintained a higher Bcl2/Bax ratio and suppressed caspase-9 expression compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastrodin; Liver sinusoidal endothelial cells; Heme oxygenase 1; Oxidative stress; Hydrogen peroxide.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000611

Insect juvenile hormone: from "status quo" to high society
Provedor de dados: 56
Autores: Hartfelder,K.
Juvenile hormone (JH) exerts pleiotropic functions during insect life cycles. The regulation of JH biosynthesis by neuropeptides and biogenic amines, as well as the transport of JH by specific binding proteins is now well understood. In contrast, comprehending its mode of action on target organs is still hampered by the difficulties in isolating specific receptors. In concert with ecdysteroids, JH orchestrates molting and metamorphosis, and its modulatory function in molting processes has gained it the attribute "status quo" hormone. Whereas the metamorphic role of JH appears to have been widely conserved, its role in reproduction has been subject to many modifications. In many species, JH stimulates vitellogenin synthesis and uptake. In mosquitoes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile hormone; Corpora allata; Metamorphosis; Insect reproduction; Polymorphism; Honey bee.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200003

The role of autolysis loop in determining the specificity of coagulation proteases
Provedor de dados: 56
Autores: Yang,L.; Manithody,C.; Rezaie,A.R.
We recently demonstrated that the substitution of the autolysis loop (residues 143 to 154 in the chymotrypsin numbering system) of activated protein C (APC) with the corresponding loop of factor Xa (fXa) renders the APC mutant (APC/fX143-154) susceptible to inhibition by antithrombin (AT) in the presence of pentasaccharide. Our recent results further indicated, that in addition to an improvement in the reactivity of APC/fX143-154 with AT, both the amidolytic and anti-factor Va activities of the mutant APC have also been significantly increased. Since the autolysis loop of APC is five residues longer than the autolysis loop of fXa, it could not be ascertained whether this loop in the mutant APC specifically interacts with the activated conformation of AT or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activated protein C; Factor Va; Antithrombin; Factor Xa; Serpins.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800005

Topographic aspects of photic driving in the electroencephalogram of children and adolescents
Provedor de dados: 56
Autores: Lazarev,V.V.; Infantosi,A.F.C.; Valencio-de-Campos,D.; Azevedo,L.C. de.
The electroencephalogram amplitude spectra at 11 fixed frequencies of intermittent photic stimulation of 3 to 24 Hz were combined into driving "profiles" for 14 scalp points in 8 male and 7 female normal subjects aged 9 to 17 years. The driving response varied over frequency and was detected in 70 to 100% of cases in the occipital areas (maximum) and in 27 to 77% of cases in the frontal areas (minimum) using as a criterion peak amplitude 20% higher than those of the neighbors. Each subject responded, on average, to 9.7 ± 1.15 intermittent photic stimulation frequencies in the right occipital area and to 6.8 ± 1.97 frequencies in the right frontal area. Most of the driving responses (in relation to the previous background) were significant according to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electroencephalogram; Intermittent photic stimulation; Photic driving; Brain topography; Children; Adolescents.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600014

Anethole reduces oxidative stress and improves in vitro survival and activation of primordial follicles
Provedor de dados: 56
Autores: Sá,N.A.R.; Bruno,J.B.; Guerreiro,D.D.; Cadenas,J.; Alves,B.G.; Cibin,F.W.S.; Leal-Cardoso,J.H.; Gastal,E.L.; Figueiredo,J.R.
Primordial follicles, the main source of oocytes in the ovary, are essential for the maintenance of fertility throughout the reproductive lifespan. To the best of our knowledge, there are no reports describing the effect of anethole on this important ovarian follicle population. The aim of the study was to investigate the effect of different anethole concentrations on the in vitro culture of caprine preantral follicles enclosed in ovarian tissue. Randomized ovarian fragments were fixed immediately (non-cultured treatment) or distributed into five treatments: α-MEM+ (cultured control), α-MEM+ supplemented with ascorbic acid at 50 μg/mL (AA), and anethole at 30 (AN30), 300 (AN300), or 2000 µg/mL (AN2000), for 1 or 7 days. After 7 days of culture, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Anethole; ROS; Primordial follicle; Caprine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800602

L- and DL-carnitine induce tetanic fade in rat neuromuscular preparation
Provedor de dados: 56
Autores: Lopes,G.; Bazotte,R.B.; Curi,R.; Alves-Do-Prado,W.
Carnitine, a structurally choline-like metabolite, has been used to increase athletic performance, although its effects on neuromuscular transmission have not been investigated. It is present in skeletal muscle and its plasma levels are about 30 to 90 µM. Using rat phrenic nerve diaphragm preparations indirectly and directly stimulated with high rate pulses, D-carnitine (30 and 60 µM), L-carnitine (60 µM) and DL-carnitine (60 µM) were shown to induce tetanic fade (D-carnitine = 19.7 ± 3.1%, N = 6; L-carnitine = 16.6 ± 2.4%, N = 6; DL-carnitine = 14.9 ± 2.1%, N = 6) without any reduction of maximal tetanic tension. D-carnitine induced tetanic fade in neuromuscular preparations previously paralyzed with d-tubocurarine and directly stimulated. The effect was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carnitine; Skeletal muscle; Tetanic fade; Atropine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900017

Cardiorespiratory and metabolic determinants during moderate and high resistance exercise intensities until exhaustion using dynamic leg press: comparison with critical load
Provedor de dados: 56
Autores: Arakelian,V.M.; Goulart,C.L.; Mendes,R.G.; Caruso,F.C.; Baldissera,V.; Arena,R.; Borghi-Silva,A.
The objective of this study was to assess cardiovascular, respiratory, and metabolic responses during a commonly used dynamic leg press resistance exercise until exhaustion (TEx) at different intensities and compare with critical load (CL). This was a prospective, cross-sectional, controlled, and crossover study. Twelve healthy young men (23±2.5 years old) participated. The subjects carried out three bouts of resistance exercise in different percentages of 1 repetition maximum (60, 75, and 90% 1RM) until TEx. CL was obtained by means of hyperbolic model and linearization of the load-duration function. During all bout intensities, oxygen uptake (VO2), carbon dioxide production (VCO2), ventilation (VE), and respiratory exchange ratio (RER) were obtained....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Load; Physiological; Muscle fatigue; Exercise; Cardiorespiratory.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100613

Synthesis, crystal structure, and anti-breast cancer activity of a novel metal-porphyrinic complex [YK(TCPP)(OH)2·(solvents)x]
Provedor de dados: 56
Autores: Liu,J-C.; Gu,J-J.; Zhang,Y-S.
A novel heterometallic metal-porphyrinic framework (MPFs) built from Y and K ions as nods and meso-tetra(4-carboxyphenyl)porphyrin as linkers has been successfully synthesized and characterized. The single crystal X-ray diffraction indicated that this complex 1 exhibited a bilayered architecture of the porphyrins, which is seldom seen in MPFs. In addition, in vitro anticancer activity of complex 1 on three human breast cancer cells (BT474, SKBr-3 and ZR-75-30) was further determined.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterometallic; Single crystal; Breast cancer cell.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100611

Genome features of Leptospira interrogans serovar Copenhageni
Provedor de dados: 56
Autores: Nascimento,A.L.T.O.; Verjovski-Almeida,S.; Van Sluys,M.A.; Monteiro-Vitorello,C.B.; Camargo,L.E.A.; Digiampietri,L.A.; Harstkeerl,R.A.; Ho,P.L.; Marques,M.V.; Oliveira,M.C.; Setubal,J.C.; Haake,D.A.; Martins,E.A.L.
We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in mammals. Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a three-member component efflux system compatible with the long-term survival of this organism. The leptospiral genome contains a broad array of genes encoding regulatory system, signal transduction and methyl-accepting chemotaxis proteins, reflecting the organism's ability to respond to diverse environmental stimuli. The identification of a complete set...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira interrogans; Outer membrane proteins; Lipopolysaccharides; Transport; Regulatory systems.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400003

A retrospective comparison of cyclophosphamide plus antithymocyte globulin with cyclophosphamide plus busulfan as the conditioning regimen for severe aplastic anemia
Provedor de dados: 56
Autores: Ommati,L.V.M.; Rodrigues,C.A.; Silva,A.R.; Silva,L.P.; Chaufaille,M.L.L.F.; Oliveira,J.S.R.
Allogeneic hematopoietic stem cell transplantation (AHSCT) is the treatment of choice for young patients with severe aplastic anemia (SAA). The association of antithymocyte globulin (ATG) and cyclophosphamide (CY) is the most frequently used conditioning regimen for this disease. We performed this retrospective study in order to compare the outcomes of HLA-matched sibling donor AHSCT in 41 patients with SAA receiving cyclophosphamide plus ATG (ATG-CY, N = 17) or cyclophosphamide plus busulfan (BU-CY, N = 24). The substitution of BU for ATG was motivated by the high cost of ATG. There were no differences in the clinical features between the two groups, including age, gender, cytomegalovirus status, ABO match, interval between diagnosis and transplant, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aplastic anemia; Allogeneic stem cell transplantation; Conditioning regimen; ATG-CY; BU-CY.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300005

Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain
Provedor de dados: 56
Autores: Horst,A.; de Souza,J.A.; Santos,M.C.Q.; Riffel,A.P.K.; Kolberg,C.; Partata,W.A.
N-acetylcysteine (NAC) inhibits nociceptive transmission. This effect has been associated partly with its antioxidant properties. However, the effect of NAC on the levels of lipid hydroperoxides (a pro-oxidant marker), content of ascorbic acid (a key antioxidant molecule of nervous tissue) and total antioxidant capacity (TAC) is unknown. Thus, our study assessed these parameters in the lumbosacral spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve, one of the most commonly employed animal models of neuropathic pain. Thirty-six male Wistar rats weighing 200–300 g were equally divided into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid hydroperoxides; Total antioxidant capacity; Ascorbic acid; Chronic constriction injury; Antinociception.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200607

Diagnostic investigation of ventilator-associated pneumonia using bronchoalveolar lavage: comparative study with a postmortem lung biopsy
Provedor de dados: 56
Autores: Balthazar,A.B.; Von Nowakonski,A.; De Capitani,E.M.; Bottini,P.V.; Terzi,R.G.G.; Araújo,S.
The purpose of the present study was to validate the quantitative culture and cellularity of bronchoalveolar lavage (BAL) for the diagnosis of ventilator-associated pneumonia (VAP). A prospective validation test trial was carried out between 1992 and 1997 in a general adult intensive care unit of a teaching hospital. Thirty-seven patients on mechanical ventilation with suspected VAP who died at most three days after a BAL diagnostic procedure were submitted to a postmortem lung biopsy. BAL effluent was submitted to Gram staining, quantitative culture and cellularity count. Postmortem lung tissue quantitative culture and histopathological findings were considered to be the gold standard exams for VAP diagnosis. According to these criteria, 20 patients (54%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nosocomial pneumonia; Mechanical ventilation; Diagnostic techniques; Bronchoalveolar lavage; Lung biopsy; Ventilator-associated pneumonia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800004

Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro
Provedor de dados: 56
Autores: Li,K.Q.; Jia,S.S.; Ma,M.; Shen,H.Z.; Xu,L.; Liu,G.P.; Huang,S.Y.; Zhang,D.S.
Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoride; Periodontitis; Periodontal ligament cells; Mineralization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800601

In vitro-induced antibody production in chronic hepatitis C virus infection
Provedor de dados: 56
Autores: Oliveira Jr.,E.B.; Ferraz,M.L.G.; Perez,R.M.; Silva,A.E.B.; Lanzoni,V.P.; Granato,C.F.
The objectives of the present study were to assess the in vitro-induced anti-hepatitis C virus (HCV) antibody production (IVIAP) in relation to the clinical, biochemical, virologic and histologic variables of patients with HCV infection. The study included 57 patients (60% males) with HCV infection (anti-HCV and HCV-RNA positive). Alanine aminotransferase (ALT) was elevated in 89% of the patients. Mean viral load was 542,241 copies/ml and histology of the liver showed chronic hepatitis in 27/52 (52%) and cirrhosis in 11/52 (21%) patients. IVIAP levels were determined by immunoenzymatic assay at median absorbance of 0.781 at 450 nm. IVIAP was negative in 14% of the patients. When groups with IVIAP levels above and below the median were compared, high IVIAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; In vitro-induced antibody production; IVIAP; Hepatitis C virus-RNA; Liver biopsy; Anti-HCV antibodies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300011

Moderate-term reproducibility of heart rate variability during rest and light to moderate exercise in children
Provedor de dados: 56
Autores: Leicht,A.S.; Allen,G.D.
Previous studies have demonstrated the high reproducibility of heart rate variability (HRV) measures in adults while little information exists concerning HRV reproducibility in children. Subsequently, the aim of the current study was to examine the moderate-term reproducibility of heart rate and frequency domain measures of HRV during rest and light to moderate exercise in children. Ten healthy children (6 males, 4 females) aged between 7 and 12 years of age volunteered for this study with HRV recordings obtained during supine rest and three treadmill walking exercise work rates (≤60% maximum heart rate), initially and then 8 weeks later. Differences (P &lt; 0.05) between variables were examined using paired t-tests or Wilcoxon signed rank tests while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic nervous system; Heart rate; Parasympathetic; Neural control; Reliability.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700013

Description of Lyme disease-like syndrome in Brazil: is it a new tick borne disease or Lyme disease variation?
Provedor de dados: 56
Autores: Mantovani,E.; Costa,I.P.; Gauditano,G; Bonoldi,V.L.N.; Higuchi,M.L.; Yoshinari,N.H.
An emerging clinical entity that reproduces clinical manifestations similar to those observed in Lyme disease (LD) has been recently under discussion in Brazil. Due to etiological and laboratory particularities it is named LD-like syndrome or LD imitator syndrome. The condition is considered to be a zoonosis transmitted by ticks of the genus Amblyomma, possibly caused by interaction of multiple fastidious microorganisms originating a protean clinical picture, including neurological, osteoarticular and erythema migrans-like lesions. When peripheral blood of patients with LD-like syndrome is viewed under a dark-field microscope, mobile uncultivable spirochete-like bacteria are observed. PCR carried out with specific or conservative primers to recognize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lyme disease-like syndrome; Tick-borne disease; Mycoplasma; Chlamydia; Spirochete; Lyme disease.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400002

Use of cardiopulmonary exercise testing to assess early ventilatory changes related to occupational particulate matter
Provedor de dados: 56
Autores: Chao,T.P.; Sperandio,E.F.; Ostolin,T.L.V.P.; Almeida,V.R.; Romiti,M.; Gagliardi,A.R.T.; Arantes,R.L.; Dourado,V.Z.
Spirometry has been used as the main strategy for assessing ventilatory changes related to occupational exposure to particulate matter (OEPM). However, in some cases, as one of its limitations, it may not be sensitive enough to show abnormalities before extensive damage, as seen in restrictive lung diseases. Therefore, we hypothesized that cardiopulmonary exercise testing (CPET) may be better than spirometry to detect early ventilatory impairment caused by OEPM. We selected 135 male workers with at least one year of exposure. After collection of self-reported socioeconomic status, educational level, and cardiovascular risk data, participants underwent spirometry, CPET, body composition assessment (bioelectrical impedance), and triaxial accelerometry (for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Occupational exposure to particulate matter; Cardiopulmonary exercise testing; Detection; Spirometry; Ventilatory impairment.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500612

Preliminary data on the prevalence of psychiatric disorders in Brazilian male and female juvenile delinquents
Provedor de dados: 56
Autores: Andrade,R.C.; Silva,V.A.; Assumpção Jr.,F.B.
The aim of the present investigation was to study the prevalence of psychiatric disorders in a sample of delinquent adolescents of both genders and to compare the prevalence between genders. A total of 116 adolescents (99 males and 17 females) aged 12 to 19 on parole in the State of Rio de Janeiro were interviewed using the screening interview based on the Schedule for Affective Disorders and Schizophrenia for School-Age Children - Present and Lifetime (KSADS-PL). Data were collected between May 2002 and January 2003. Of 373 male and 58 female adolescents present in May 2002 in the largest institution that gives assistance to adolescents on parole in the city of Rio de Janeiro, 119 subjects were assessed (three of them refused to participate). Their...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Delinquency; Conduct disorder; Psychiatry disorders; Adolescents; Parolees; Illicit drug use.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800005

Lack of a relationship between serum ferritin levels and coronary atherosclerosis evaluated by coronary arteriography
Provedor de dados: 56
Autores: Manfroi,W.C.; Zago,A.J.; Cruz,R.; Oliveira,J.; Kirschnick,L.S.; Ordovás,K.; Candiago,R.H.; Souza,J.; Ribeiro,L.W.; Leitão,C.; Brizolara,M.L.
Many clinical and epidemiological studies have demonstrated the relationship between serum ferritin and ischemic heart disease. In the present study we evaluated the relationship between coronary heart disease (CHD) and serum ferritin levels in patients submitted to coronary arteriography. We evaluated 307 patients (210 (68.7%) males; median age: 60 years) who were submitted to coronary angiography, measurement of serum ferritin and identification of clinical events of ischemic heart disease. Serum ferritin is reported as quartiles. Ninety-six patients (31.27%) had normal coronary angiography (group 1) and 211 (68.73%) had coronary heart disease (group 2). Of the patients with CHD, 61 (28.9%) had serum ferritin levels higher than 194 ng/ml (4th quartile),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ferritin; Coronary heart disease; Atherosclerosis; Risk factors; Coronary arteriography.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300009

Role of nitric oxide and superoxide in Giardia lamblia killing
Provedor de dados: 56
Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (~60% dead trophozoites). This effect was inhibited (&amp;gt;90%) by an NO synthase inhibitor (200 µM) and unaffected by superoxide dismutase (SOD, 300 U/ml). Giardia trophozoites were killed by the NO donors, S-nitroso-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP) in a dose-dependent manner (LD50 300 and 50 µM, respectively). A dual NO-superoxide anion donor, 3-morpholino-sydnonimine hydrochloride (SIN-1), did not have a killing effect in concentrations up to 1 mM. However, when SOD (300 U/ml)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Macrophage; Peroxynitrite; Giardia lamblia; Cytotoxicity; Superoxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100015

Methylophiopogonanone A, an Ophiopogon homoisoflavonoid, alleviates high-fat diet-induced hyperlipidemia: assessment of its potential mechanism
Provedor de dados: 56
Autores: Li,Zhao; Wu,Ying-Ying; Yu,Bei-Xin.
Methylophiopogonanone A (MO-A), a homoisoflavonoid extracted from Ophiopogon japonicus, has been shown to attenuate myocardial apoptosis and improve cerebral ischemia/reperfusion injury. However, the hypolipidemic effects remain unknown. This study was performed to investigate a potential hypolipidemic effect of MO-A in hyperlipidemia rats, as well as its underlying mechanism of action. A rat model of hyperlipidemia was induced by a high-fat diet (HFD). Animals were randomly divided into three groups (n=8/group): normal control group (NC), HFD group, and HFD+MO-A (10 mg·kg-1·d-1) treatment group. The effects of MO-A on serum lipids, body weight, activity of lipoprotein metabolism enzyme, and gene expression of lipid metabolism were evaluated in HFD-induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ophiopogon japonicus; PPARα; High-fat diet; Lipid lowering; Dyslipidemia.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300609

Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C
Provedor de dados: 56
Autores: de Almeida,R.W.; Espírito-Santo,M.P.; Sousa,P.S.F.; de Almeida,A.J.; Lampe,E.; Lewis-Ximenez,L.L.
We evaluated the stability of hepatitis B virus (HBV) DNA in plasma samples stored at 42°C for external quality assessment (EQA) panels of viral load. To assess the stability of plasma samples containing different concentrations of HBV DNA, serial dilutions of HBV-infected samples with a viral load of 6.40 log(10) IU/mL were made to yield viral loads of 5, 4, and 3 log(10) IU/mL. These were incubated at 42°C for up to 7 days and then frozen at -70°C. Viral load testing for HBV DNA was performed for all samples using COBAS¯ AmpliPrep/COBAS¯ TaqMan¯ HBV Test (v.2.0, Roche, Switzerland). Results were compared with fresh frozen plasma samples as a benchmark to establish acceptable measurements on the days following sample collection. Although the results of...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: HBV; Stability studies; Real-time PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600553

Control of CD4 gene expression: connecting signals to outcomes in T cell development
Provedor de dados: 56
Autores: Sarafova,S.D.; Siu,G.
The control of CD4 gene expression is essential for proper T lymphocyte development. Signals transmitted from the T-cell antigen receptor (TCR) during the thymic selection processes are believed to be linked to the regulation of CD4 gene expression during specific stages of T cell development. Thus, a study of the factors that control CD4 gene expression may lead to further insight into the molecular mechanisms that drive thymic selection. In this review, we discuss the work conducted to date to identify and characterize the cis-acting transcriptional control elements in the CD4 locus and the DNA-binding factors that mediate their function. From these studies, it is becoming clear that the molecular mechanisms controlling CD4 gene expression are very...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD4; Gene expression; Silencer; T cell; Development.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700001

Comparison of endoplasmic reticulum stress and mitochondrial biogenesis responses after 12 weeks of treadmill running and ladder climbing exercises in the cardiac muscle of middle-aged obese rats
Provedor de dados: 56
Autores: Kim,Kijin; Ahn,Nayoung; Jung,Suryun.
The purpose of the present study was to compare the influence of aerobic exercise (AE) lasting 12 weeks to that of resistance exercise (RE) of the same duration on endoplasmic reticulum (ER) stress and mitochondrial biogenesis in the cardiac muscle of middle-aged obese rats. Obesity was induced in thirty 50-week-old male Sprague Dawley rats over 6 weeks by administration of a high-fat diet. The rats were then subjected to treadmill-running (AE) and ladder-climbing (RE) exercises 3 times per week for 12 weeks. Rats in the AE group showed significantly lower increases in body weight and intraperitoneal fat than those in the sedentary control (SC) group (P&lt;0.05). The 12-week exercise regimes resulted in a significant increase in expression of mitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; Mitochondrial biogenesis; Endoplasmic reticulum stress; Obesity; Cardiac muscle; Aging.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000604

Occult hepatitis B virus infection in liver transplant patients in a Brazilian referral center
Provedor de dados: 56
Autores: Ferrari,T.C.A.; Xavier,M.A.P.; Vidigal,P.V.T.; Amaral,N.S.; Diniz,P.A.; Resende,A.P.; Miranda,D.M.; Faria,A.C.; Lima,A.S.; Faria,L.C.
Estimates of occult hepatitis B virus (HBV) infection prevalence varies among different studies depending on the prevalence of HBV infection in the study population and on the sensitivity of the assay used to detect HBV DNA. We investigated the prevalence of occult HBV infection in cirrhotic patients undergoing liver transplantation in a Brazilian referral center. Frozen liver samples from 68 adults were analyzed using a nested polymerase chain reaction assay for HBV DNA. The specificity of the amplified HBV sequences was confirmed by direct sequencing of the amplicons. The patient population comprised 49 (72.1%) males and 19 (27.9%) females with a median age of 53 years (range=18-67 years). Occult HBV infection was diagnosed in three (4.4%) patients. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Occult hepatitis B virus infection; Hepatitis B virus; HBV DNA; Liver transplantation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100990

Apoptotic mimicry: an altruistic behavior in host/Leishmania interplay
Provedor de dados: 56
Autores: Wanderley,J.L.M.; Benjamin,A.; Real,F.; Bonomo,A.; Moreira,M.E.C.; Barcinski,M.A.
Apoptosis is the most common phenotype observed when cells die through programmed cell death. The morphologic and biochemical changes that characterize apoptotic cells depend on the activation of a diverse set of genes. Apoptosis is essential for multicellular organisms since their development and homeostasis are dependent on extensive cell renewal. In fact, there is strong evidence for the correlation between the emergence of multicellular organisms and apoptosis during evolution. On the other hand, no obvious advantages can be envisaged for unicellular organisms to carry the complex machinery required for programmed cell death. However, accumulating evidence shows that free-living and parasitic protozoa as well as yeasts display apoptotic markers. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Phosphatidylserine; Leishmania; Phagocyte.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600001

Astroglial cells derived from lateral and medial midbrain sectors differ in their synthesis and secretion of sulfated glycosaminoglycans
Provedor de dados: 56
Autores: Onofre,G.R.; Werneck,C.C.; Mendes,F.A.; Garcia-Abreu,J.; Moura Neto,V.; Cavalcante,L.A.; Silva,L.C.F.
Astroglial cells derived from lateral and medial midbrain sectors differ in their abilities to support neuritic growth of midbrain neurons in cocultures. These different properties of the two types of cells may be related to the composition of their extracellular matrix. We have studied the synthesis and secretion of sulfated glycosaminoglycans (GAGs) by the two cell types under control conditions and ß-D-xyloside-stimulated conditions, that stimulate the ability to synthesize and release GAGs. We have confirmed that both cell types synthesize and secrete heparan sulfate and chondroitin sulfate. Only slight differences were observed between the proportions of the two GAGs produced by the two types of cells after a 24-h labeling period. However, a marked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycosaminoglycans; Astrocytes; Neuron-glia interactions; Midbrain.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200014

Extracellular matrix molecules as targets for brown spider venom toxins
Provedor de dados: 56
Autores: Veiga,S.S.; Zanetti,V.C.; Braz,A.; Mangili,O.C.; Gremski,W.
Loxoscelism, the term used to describe lesions and clinical manifestations induced by brown spider's venom (Loxosceles genus), has attracted much attention over the last years. Brown spider bites have been reported to cause a local and acute inflammatory reaction that may evolve to dermonecrosis (a hallmark of envenomation) and hemorrhage at the bite site, besides systemic manifestations such as thrombocytopenia, disseminated intravascular coagulation, hemolysis, and renal failure. The molecular mechanisms by which Loxosceles venoms induce injury are currently under investigation. In this review, we focused on the latest reports describing the biological and physiopathological aspects of loxoscelism, with reference mainly to the proteases recently...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brown spider; Venom; Extracellular matrix; Proteolytic effect.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700002

Visual and automatic classification of the cyclic alternating pattern in electroencephalography during sleep
Provedor de dados: 56
Autores: Largo,R.; Lopes,M.C.; Spruyt,K.; Guilleminault,C.; Wang,Y.P.; Rosa,A.C.
Cyclic alternating pattern (CAP) is a neurophysiological pattern that can be visually scored by international criteria. The aim of this study was to verify the feasibility of visual CAP scoring using only one channel of sleep electroencephalogram (EEG) to evaluate the inter-scorer agreement in a variety of recordings, and to compare agreement between visual scoring and automatic scoring systems. Sixteen hours of single-channel European data format recordings from four different sleep laboratories with either C4-A1 or C3-A2 channels and with different sampling frequencies were used in this study. Seven independent scorers applied visual scoring according to international criteria. Two automatic blind scorings were also evaluated. Event-based inter-scorer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclic alternating pattern; Sleep; Visual scoring; Automatic scoring.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300602

Heart rate variability under resting conditions in postmenopausal and young women
Provedor de dados: 56
Autores: Ribeiro,T.F.; Azevedo,G.D.; Crescêncio,J.C.; Marães,V.R.F.S.; Papa,V.; Catai,A.M.; Verzola,R.M.M.; Oliveira,L.; Silva de Sá,M.F.; Gallo Jr.,L.; Silva,E.
The aim of the present study was to compare the modulation of heart rate in a group of postmenopausal women to that of a group of young women under resting conditions on the basis of R-R interval variability. Ten healthy postmenopausal women (mean ± SD, 58.3 ± 6.8 years) and 10 healthy young women (mean ± SD, 21.6 ± 0.82 years) were submitted to a control resting electrocardiogram (ECG) in the supine and sitting positions over a period of 6 min. The ECG was obtained from a one-channel heart monitor at the CM5 lead and processed and stored using an analog to digital converter connected to a microcomputer. R-R intervals were calculated on a beat-to-beat basis from the ECG recording in real time using a signal-processing software. Heart rate variability (HRV)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart rate variability; Menopause; Autonomic nervous system; Time domain indexes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700006

Pharmacogenetic polymorphisms in Brazilian-born, first-generation Japanese descendants
Provedor de dados: 56
Autores: Perini,J.A.; Vargens,D.D.; Santana,I.S.C.; Moriguchi,E.H.; Ribeiro-dos-Santos,A.K.C.; Tsutsumi,M.; Suarez-Kurtz,G.
Brazil hosts the largest Japanese community outside Japan, estimated at 1.5 million individuals, one third of whom are first-generation, Brazilian-born with native Japanese parents. This large community provides a unique opportunity for comparative studies of the distribution of pharmacogenetic polymorphisms in native Japanese versus their Brazilian-born descendants. Functional polymorphisms in genes that modulate drug disposition (CYP2C9, CYP2C19 and GSTM3) or response (VKORC1) and that differ significantly in frequency in native Japanese versus Brazilians with no Japanese ancestry were selected for the present study. Healthy subjects (200 native Japanese and 126 first-generation Japanese descendants) living in agricultural colonies were enrolled....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Japanese; Brazil; CYP2C9; CYP2C19; GSTM3; VKORC1.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200010

The decrease in uroporphyrinogen decarboxylase activity induced by ethanol predisposes rats to the development of porphyria and accelerates xenobiotic-triggered porphyria, regardless of hepatic damage
Provedor de dados: 56
Autores: Ríos de Molina,M.C.; Mazzetti,M.B.; Galigniana,M.; Aldonatti,C.; Tomio,J.M.; San Martín de Viale,L.C.
We evaluated the porphyrinogenic ability of ethanol (20% in drinking water) per se, its effect on the development of sporadic porphyria cutanea tarda induced by hexachlorobenzene in female Wistar rats (170-190 g, N = 8/group), and the relationship with hepatic damage. Twenty-five percent of the animals receiving ethanol increased up to 14-, 25-, and 4.5-fold the urinary excretion of delta-aminolevulinate, porphobilinogen, and porphyrins, respectively. Ethanol exacerbated the precursor excretions elicited by hexachlorobenzene. Hepatic porphyrin levels increased by hexachlorobenzene treatment, while this parameter only increased (up to 90-fold) in some of the animals that received ethanol alone. Ethanol reduced the activities of uroporphyrinogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholism; Ethanol; Heme metabolic pathway; Hepatic damage; Hexachlorobenzene; Porphyria cutanea tarda.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100004

Non-clinical studies required for new drug development - Part I: early in silico and in vitro studies, new target discovery and validation, proof of principles and robustness of animal studies
Provedor de dados: 56
Autores: Andrade,E.L.; Bento,A.F.; Cavalli,J.; Oliveira,S.K.; Freitas,C.S.; Marcon,R.; Schwanke,R.C.; Siqueira,J.M.; Calixto,J.B.
This review presents a historical overview of drug discovery and the non-clinical stages of the drug development process, from initial target identification and validation, through in silico assays and high throughput screening (HTS), identification of leader molecules and their optimization, the selection of a candidate substance for clinical development, and the use of animal models during the early studies of proof-of-concept (or principle). This report also discusses the relevance of validated and predictive animal models selection, as well as the correct use of animal tests concerning the experimental design, execution and interpretation, which affect the reproducibility, quality and reliability of non-clinical studies necessary to translate to and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-clinical drug discovery and development; Target discovery and validation; Proof of principles; Animal research robustness.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100301

Effects of disturbed blood flow during exercise on endothelial function: a time course analysis
Provedor de dados: 56
Autores: Paiva,F.M.; Vianna,L.C.; Fernandes,I.A.; Nóbrega,A.C.; Lima,R.M.
This study aimed to examine the time course of endothelial function after a single handgrip exercise session combined with blood flow restriction in healthy young men. Nine participants (28±5.8 years) completed a single session of bilateral dynamic handgrip exercise (20 min with 60% of the maximum voluntary contraction). To induce blood flow restriction, a cuff was placed 2 cm below the antecubital fossa in the experimental arm. This cuff was inflated to 80 mmHg before initiation of exercise and maintained through the duration of the protocol. The experimental arm and control arm were randomly selected for all subjects. Brachial artery flow-mediated dilation (FMD) and blood flow velocity profiles were assessed using Doppler ultrasonography before...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood flow restriction; Vascular function; Flow-mediated dilation; Shear stress.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400701

Expression of neuronal nitric oxide synthase in the hippocampal formation in affective disorders
Provedor de dados: 56
Autores: Oliveira,R.M.W.; Guimarães,F.S.; Deakin,J.F.W.
Hippocampal output is increased in affective disorders and is mediated by increased glutamatergic input via N-methyl-D-aspartate (NMDA) receptor and moderated by antidepressant treatment. Activation of NMDA receptors by glutamate evokes the release of nitric oxide (NO) by the activation of neuronal nitric oxide synthase (nNOS). The human hippocampus contains a high density of NMDA receptors and nNOS-expressing neurons suggesting the existence of an NMDA-NO transduction pathway which can be involved in the pathogenesis of affective disorders. We tested the hypothesis that nNOS expression is increased in the human hippocampus from affectively ill patients. Immunocytochemistry was used to demonstrate nNOS-expressing neurons in sections obtained from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Affective disorders; Major depression; Neuronal nitric oxide synthase; Immunohistochemistry; Hippocampus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400012

In vitro inhibitory effects of imatinib mesylate on stromal cells and hematopoietic progenitors from bone marrow
Provedor de dados: 56
Autores: Soares,P.B.; Jeremias,T.S.; Alvarez-Silva,M.; Licínio,M.A.; Santos-Silva,M.C.; Vituri,C.L.
Imatinib mesylate (IM) is used to treat chronic myeloid leukemia (CML) because it selectively inhibits tyrosine kinase, which is a hallmark of CML oncogenesis. Recent studies have shown that IM inhibits the growth of several non-malignant hematopoietic and fibroblast cells from bone marrow (BM). The aim of the present study was to evaluate the effects of IM on stromal and hematopoietic progenitor cells, specifically in the colony-forming units of granulocyte/macrophage (CFU-GM), using BM cultures from 108 1.5- to 2-month-old healthy Swiss mice. The results showed that low concentrations of IM (1.25 µM) reduced the growth of CFU-GM in clonogenic assays. In culture assays with stromal cells, fibroblast proliferation and α-SMA expression by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow stromal cell; Hematopoietic progenitors; Imatinib mesylate; Myelosuppression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100039

Genetic variation of aggrecanase-2 (ADAMTS5) in susceptibility to osteoarthritis
Provedor de dados: 56
Autores: Zhou,Xindie; Jiang,Lifeng; Zhang,Yi; Zhang,Junjie; Zhou,Dong; Wu,Lidong; Huang,Yong; Xu,Nanwei.
Aggrecanase-2 (ADAMTS5) gene is responsible for aggrecan degradation that may contribute to cartilage destruction in a mouse osteoarthritis (OA) model. We aimed to investigate the effects of ADAMTS5 gene polymorphisms on OA risk in a Chinese population. A total of 300 OA patients and 300 controls were recruited and their genotypes for ADAMTS5 gene rs226794 and rs2830585 polymorphisms were determined using a custom-by-design 48-Plex single nucleotide polymorphism Scan™ kit. ADAMTS5-associated genes were identified by co-expression analysis and their functions were investigated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. Bioinformatics analysis showed that ADAMTS5 was significantly related to the components, structural constituent,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADAMTS5; Polymorphism; Osteoarthritis; Co-expression analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200602

Psychopharmacotherapy of panic disorder: 8-week randomized trial with clonazepam and paroxetine
Provedor de dados: 56
Autores: Nardi,A.E.; Valença,A.M.; Freire,R.C.; Mochcovitch,M.D.; Amrein,R.; Sardinha,A.; Levitan,M.N.; Nascimento,I.; de-Melo-Neto,V.L.; King,A.L.; de O. e Silva,A.C.; Veras,A.B.; Dias,G.P.; Soares-Filho,G.L.; da Costa,R.T.; Mezzasalma,M.A.; de Carvalho,M.R.; de Cerqueira,A.C.; Hallak,J.E.; Crippa,J.A.; Versiani,M.
The objective of the present randomized, open-label, naturalistic 8-week study was to compare the efficacy and safety of treatment with clonazepam (N = 63) and paroxetine (N = 57) in patients with panic disorder with or without agoraphobia. Efficacy assessment included number of panic attacks and clinician ratings of the global severity of panic disorders with the clinical global impression (CGI) improvement (CGI-I) and CGI severity (CGI-S) scales. Most patients were females (69.8 and 68.4% in the clonazepam and paroxetine groups, respectively) and age (mean ± SD) was 35.9 ± 9.6 years for the clonazepam group and 33.7 ± 8.8 years for the paroxetine group. Treatment with clonazepam versus paroxetine resulted in fewer weekly panic attacks at week 4 (0.1 vs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paroxetine; Clonazepam; Panic disorder; Efficacy; Safety.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400015

Angiotensin II-mediated vascular smooth muscle cell growth signaling
Provedor de dados: 56
Autores: Inagami,T.; Eguchi,S.
The mechanism by which Ang II stimulates the growth of vascular smooth muscle cells was investigated by measuring the phosphorylation of mitogen-activated protein kinases ERK 1 and ERK 2. Ca2+ ionophore was found to have effects practically analogous to Ang II. We found that the signaling pathway involves the activation of epidermal growth factor receptor (EGFR) kinase, activation of the adaptor proteins Shc and Grb2, and the small G-protein Ras. Although the mechanism of AT1- (or Ca2+)-induced activation of EGFR is not yet clear, we have found that calcium-dependent protein kinase CAKß/PYK2 and c-Src are involved in this process. These studies indicate a transactivation mechanism that utilizes EGFR as a bridge between a Gq-coupled receptor and activation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Type 1 receptor; Epidermal growth factor receptor; Vascular smooth muscle cells.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600002

The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells
Provedor de dados: 56
Autores: Fan,J.Z.; Yang,X.; Bi,Z.G.
We investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different osteogenic markers and cytokines was analyzed by real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. In addition, alkaline phosphatase (ALP) enzyme activity and biomineralization as markers for differentiation were measured. Treatment with 6-gingerol resulted in insignificant effects on the proliferation rate. 6-Gingerol induced the differentiation of osteoblast-like cells with increased transcription levels of osteogenic markers, upregulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 6-Gingerol; Interleukin-6; Nuclear factor-κB; Osteogenesis; Osteoblast.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700637

Changes of ongoing activity in Cebus monkey perirhinal cortex correlate with behavioral performance
Provedor de dados: 56
Autores: Lima,B.; Fiorani,M.; Gattass,R.
A Cebus apella monkey weighing 4 kg was trained in a saccadic eye movement task and while the animal performed the task we recorded the extracellular activity of perirhinal cortical neurons. Although the task was very simple and maintained at a constant level of difficulty, we observed considerable changes in the performance of the monkey within each experimental session. The behavioral states responsible for such variation may be related to arousal, motivation or attention of the animal while engaged in the task. In approximately 20% (16/82) of the units recorded, long-term direct or inverse correlations could be demonstrated between the monkey's behavioral state and the cells' ongoing activity (independent of the visual stimulation or of the specific...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Perirhinal cortex; Behavioral states; Motivation; Cebus monkeys.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100010

A third generation regimen VACOP-B with or without adjuvant radiotherapy for aggressive localized non-Hodgkin's lymphoma: report from the Italian Non-Hodgkin's Lymphoma Co-operative Study Group
Provedor de dados: 56
Autores: Santini,G.; De Souza,C.; Aversa,S.; Patti,C.; Tedeschi,L.; Candela,M.; Olivieri,A.; Chisesi,T.; Rubagotti,A.; Centurioni,R.; Nardi,V.; Congiu,M.; Gennaro,M.; Truini,M.
The objective of this multicenter prospective study was to determine the clinical efficacy and toxicity of a polychemotherapeutic third generation regimen, VACOP-B, with or without radiotherapy as front-line therapy in aggressive localized non-Hodgkin's lymphoma. Ninety-three adult patients (47 males and 46 females, median age 45 years) with aggressive localized non-Hodgkin's lymphoma, 43 in stage I and 50 in stage II (non-bulky), were included in the study. Stage I patients received VACOP-B for 6 weeks plus involved field radiotherapy and stage II patients received 12 weeks VACOP-B plus involved field radiotherapy on residual masses. Eighty-six (92.5%) achieved complete remission and 4 (4.3%) partial remission. Three patients (3.2%) were primarily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Localized aggressive non-Hodgkin's lymphoma; VACOP-B; Radiotherapy; Toxicity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500014

Propofol inhibits lung cancer cell viability and induces cell apoptosis by upregulating microRNA-486 expression
Provedor de dados: 56
Autores: Yang,N.; Liang,Y.; Yang,P.; Yang,T.; Jiang,L.
Propofol is a frequently used intravenous anesthetic agent. Recent studies show that propofol exerts a number of non-anesthetic effects. The present study aimed to investigate the effects of propofol on lung cancer cell lines H1299 and H1792 and functional role of microRNA (miR)-486 in these effects. H1299 and/or H1792 cells were treated with or without propofol and transfected or not with miR-486 inhibitor, and then cell viability and apoptosis were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry. The expression of miR-486 was determined by quantitative real-time polymerase chain reaction (qRT-PCR) with or without propofol treatment. Western blot was performed to analyze the protein expression of Forkhead...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; Lung cancer; Cell viability; Cell apoptosis; MicroRNA-486.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100601

Functional characterization and localization of AQP3 in the human colon
Provedor de dados: 56
Autores: Silberstein,C.; Kierbel,A.; Amodeo,G.; Zotta,E.; Bigi,F.; Berkowski,D.; Ibarra,C.
Water channels or aquaporins (AQPs) have been identified in a large variety of tissues. Nevertheless, their role in the human gastrointestinal tract, where their action is essential for the reabsorption and secretion of water and electrolytes, is still unclear. The purpose of the present study was to investigate the structure and function of water channels expressed in the human colon. A cDNA fragment of about 420 bp with a 98% identity to human AQP3 was amplified from human stomach, small intestine and colon by reverse transcription polymerase chain reaction (RT-PCR) and a transcript of 2.2 kb was expressed more abundantly in colon than in jejunum, ileum and stomach as indicated by Northern blots. Expression of mRNA from the colon of adults and children...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Absorptive epithelium; Human colon; Water channels; Aquaporins; AQP3.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000018

The interaction of meal-related, rhythmic and homeostatic mechanisms and the generation of thirst and drinking
Provedor de dados: 56
One of the primary goals of the study of thirst is to understand why drinking occurs under ad libitum or natural conditions. An appreciation of the experimental strategies applied by physiologists studying thirst from different perspectives can facilitate progress toward understanding the natural history of drinking behavior. Drinking research carried out using three separate perspectives - homeostatic, circadian rhythms, and food-associated - generates types of information about the mechanisms underlying drinking behavior. By combining research strategies and methods derived from each of these approaches, it has been possible to gain new information that increases our appreciation of the interactions between homeostatic mechanisms and circadian rhythms in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Homeostatic thirst; Cellular dehydration; Extracellular dehydration; Circadian rhythms; Meal-related drinking.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400009

Comparison of the reliability of multifocal visual evoked cortical potentials generated by pattern reversal and pattern pulse stimulation
Provedor de dados: 56
Autores: Souza,G.S.; Schakelford,H.B.; Moura,A.L.A.; Gomes,B.D.; Ventura,D.F.; Fitzgerald,M.E.C.; Silveira,L.C.L.
This study compared the effectiveness of the multifocal visual evoked cortical potentials (mfVEP) elicited by pattern pulse stimulation with that of pattern reversal in producing reliable responses (signal-to-noise ratio &gt;1.359). Participants were 14 healthy subjects. Visual stimulation was obtained using a 60-sector dartboard display consisting of 6 concentric rings presented in either pulse or reversal mode. Each sector, consisting of 16 checks at 99% Michelson contrast and 80 cd/m² mean luminance, was controlled by a binary m-sequence in the time domain. The signal-to-noise ratio was generally larger in the pattern reversal than in the pattern pulse mode. The number of reliable responses was similar in the central sectors for the two stimulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multifocal visual evoked cortical potential; Contrast vision; Spatial vision; Signal-to-noise analysis; Visual electrophysiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000011

Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm
Provedor de dados: 56
Autores: Brito,C.F.A.; Oliveira,G.C.; Oliveira,S.C.; Street,M.; Riengrojpitak,S.; Wilson,R.A.; Simpson,A.J.G.; Correa-Oliveira,R.
Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sm14; Fatty acid-binding protein; FABP; Immunolocalization; RT-PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014

Immunological effects of donor lymphocyte infusion in patients with chronic myelogenous leukemia relapsing after bone marrow transplantation
Provedor de dados: 56
Autores: Castro,F.A.; Palma,P.V.B.; Morais,F.R.; Voltarelli,J.C.
Allogeneic bone marrow transplantation (alloBMT) is the only curative therapy for chronic myelogenous leukemia (CML). This success is explained by the delivery of high doses of antineoplastic agents followed by the rescue of marrow function and the induction of graft-versus-leukemia reaction mediated by allogeneic lymphocytes against host tumor cells. This reaction can also be induced by donor lymphocyte infusion (DLI) producing remission in most patients with CML who relapse after alloBMT. The immunological mechanisms involved in DLI therapy are poorly understood. We studied five CML patients in the chronic phase, who received DLI after relapsing from an HLA-identical BMT. Using flow cytometry we evaluated cellular activation and apoptosis, NK...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chronic myelogenous leukemia; Donor lymphocyte infusion; Immune system; Bone marrow transplantation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200006

The terrestrial Gastropoda Megalobulimus abbreviatus as a useful model for nociceptive experiments: effects of morphine and naloxone on thermal avoidance behavior
Provedor de dados: 56
Autores: Achaval,M.; Penha,M.A.P.; Swarowsky,A.; Rigon,P.; Xavier,L.L.; Viola,G.G.; Zancan,D.M.
We describe the behavior of the snail Megalobulimus abbreviatus upon receiving thermal stimuli and the effects of pretreatment with morphine and naloxone on behavior after a thermal stimulus, in order to establish a useful model for nociceptive experiments. Snails submitted to non-functional (22ºC) and non-thermal hot-plate stress (30ºC) only displayed exploratory behavior. However, the animals submitted to a thermal stimulus (50ºC) displayed biphasic avoidance behavior. Latency was measured from the time the animal was placed on the hot plate to the time when the animal lifted the head-foot complex 1 cm from the substrate, indicating aversive thermal behavior. Other animals were pretreated with morphine (5, 10, 20 mg/kg) or naloxone (2.5, 5.0, 7.5 mg/kg)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snail; Thermal avoidance; Nociception; Morphine; Naloxone.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100012

Water extracts of cabbage and kale inhibit ex vivo H2O2-induced DNA damage but not rat hepatocarcinogenesis
Provedor de dados: 56
Autores: Horst,M.A.; Ong,T.P.; Jordão Jr.,A.A.; Vannucchi,H.; Moreno,F.S.; Lajolo,F.M.
The chemopreventive potential of water extracts of the Brassica vegetables cabbage and kale was evaluated by administering their aqueous extracts in drinking water ad libitum to Wistar rats submitted to Ito’s hepatocarcinogenesis model (CB group and K group, respectively - 14 rats per group). Animals submitted to this same model and treated with water were used as controls (W group - 15 rats). Treatment with the vegetable extracts did not inhibit (P &gt; 0.05) placental glutathione S-transferase-positive preneoplastic lesions (PNL). The number of apoptotic bodies did not differ (P &gt; 0.05) among the experimental groups. Ex vivo hydrogen peroxide treatment of rat livers resulted in lower (P &lt; 0.05) DNA strand breakage in cabbage- (107.6 ± 7.8 µm) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemoprevention; Hepatocarcinogenesis; Diethylnitrosamine; Brassica vegetables; DNA damage.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300004

Improvement of bovine ß-lactoglobulin production and secretion by Lactococcus lactis
Provedor de dados: 56
Autores: Nouaille,S.; Bermúdez-Humarán,L.G.; Adel-Patient,K.; Commissaire,J.; Gruss,A.; Wal,J.M.; Azevedo,V.; Langella,P.; Chatel,J.M.
The stabilizing effects of staphylococcal nuclease (Nuc) and of a synthetic propeptide (LEISSTCDA, hereafter called LEISS) on the production of a model food allergen, bovine ß-lactoglobulin (BLG), in Lactococcus lactis were investigated. The fusion of Nuc to BLG (Nuc-BLG) results in higher production and secretion of the hybrid protein. When LEISS was fused to BLG, the production of the resulting protein LEISS-BLG was only slightly improved compared to the one obtained with Nuc-BLG. However, the secretion of LEISS-BLG was dramatically enhanced (~10- and 4-fold higher than BLG and Nuc-BLG, respectively). Finally, the fusion of LEISS to Nuc-BLG resulting in the protein LEISS-Nuc-BLG led to the highest production of the hybrid protein, estimated at ~8 µg/ml...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactococcus lactis; Protein secretion; LEISSTCDA; Staphylococcal nuclease; SS-lactoglobulin; Allergen.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300005

Effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology
Provedor de dados: 56
Autores: Gomes,A.R.S.; Coutinho,E.L.; França,C.N.; Polonio,J.; Salvini,T.F.
We determined the effect of stretching applied once a week to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Twenty-six male Wistar rats weighing 269 ± 26 g were divided into three groups. Group I, the left soleus was immobilized in the shortened position for 3 weeks; group II, the soleus was immobilized in the shortened position and stretched once a week for 3 weeks; group III, the soleus was submitted only to stretching once a week for 3 weeks. The medial part of the soleus muscle was frozen for histology and muscle fiber area evaluation and the lateral part was used for the determination of number and length of serial sarcomeres. Soleus muscle submitted only to immobilization showed a reduction in weight (44 ± 6%, P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle; Stretching; Immobilization; Sarcomere number; Muscle fiber area.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000005

Morphological changes of the jejunal mucosa in protracted diarrhea and their correlation with disease duration, weight loss and serum albumin levels
Provedor de dados: 56
Autores: Péret-Filho,L.A.; Brasileiro-Filho,G.; Penna,F.J.
The pathogenesis of protracted diarrhea is multifactorial. In developing countries, intestinal infectious processes seem to play an important role in triggering the syndrome. Thirty-four children aged 1 to 14 months, mean 6.5 months, with protracted diarrhea were studied clinically and in terms of small intestinal mucosal morphology. Mild, moderate or severe hypotrophy of the jejunal mucosa was detected in 82% of cases, and mucosal atrophy was observed in 12%. The intensity of the morphological changes of the jejunal mucosa correlated negatively with serum albumin levels. No correlation was detected between mucosal grading and duration of diarrhea or between mucosal grading and weight reported as percentile. After nutritional support was instituted, serial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protracted diarrhea; Biopsy; Jejunal mucosa; Serum albumin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900004

Investigation of single-strand conformational polymorphism of the TP53 gene in women with a family history of breast cancer
Provedor de dados: 56
Autores: Burbano,R.R.; Medeiros,A.C.; Mello,A.A.; Lemos,J.A.; Bahia,M.O.; Casartelli,C.
Breast cancer in families with germ line mutations in the TP53 gene has been described in the medical literature. Mutation screening for susceptibility genes should allow effective prophylactic and preventive measures. Using single-strand conformational polymorphism, we screened for mutations in exons 5, 6, 7 and 8 of gene TP53 in the peripheral blood of 8 young non-affected members (17 to 36 years old) of families with a history of breast cancer. Studies of this type on young patients (mean age, 25 years) are very rare in the literature. The identification of these mutations would contribute to genetic counseling of members of families with predisposition to breast cancer. The results obtained did not show any polymorphism indicating mutation. In our...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: TP53; Family history of breast cancer; Mutation screening; Early breast cancer.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100010

Effect of hydroxyurea on the intracellular multiplication of Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi
Provedor de dados: 56
Autores: Melo,E.J.T.; Beiral,H.J.
Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi are obligate intracellular parasites that multiply until lysis of host cells. The present study was undertaken to evaluate the effect of hydroxyurea (an inhibitor of cell division at the G1/S phase) on the multiplication of L. amazonensis, T. gondii, and T. cruzi in infected host cells. Infected cells were treated with hydroxyurea (4 mM) for 48 h. Hydroxyurea arrested intracellular multiplication of all infective forms of the parasites tested. In treated cultures, the percent of infected host cells decreased (50-97%) and most intracellular parasites were eliminated. Ultrastructural observations showed no morphologic change in host cells while intracellular parasites presented drastic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Trypanosoma cruzi; Leishmania amazonensis; Hydroxyurea.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100009

Intensity and physiological responses to the 6-minute walk test in middle-aged and older adults: a comparison with cardiopulmonary exercise testing
Provedor de dados: 56
Autores: Sperandio,E.F.; Arantes,R.L.; Matheus,A.C.; Silva,R.P.; Lauria,V.T.; Romiti,M.; Gagliardi,A.R.T.; Dourado,V.Z.
The 6-minute walk test (6MWT) is a simple field test that is widely used in clinical settings to assess functional exercise capacity. However, studies with healthy subjects are scarce. We hypothesized that the 6MWT might be useful to assess exercise capacity in healthy subjects. The purpose of this study was to evaluate 6MWT intensity in middle-aged and older adults, as well as to develop a simple equation to predict oxygen uptake (      V ˙ O 2) from the 6-min walk distance (6MWD). Eighty-six participants, 40 men and 46 women, 40-74 years of age and with a mean body mass index of 28±6 kg/m2, performed the 6MWT according to American Thoracic Society guidelines. Physiological responses were evaluated during the 6MWT using a K4b2 Cosmed telemetry gas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Walking; Gas exchange.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400349

Experimental models in vaccine research: malaria and leishmaniasis
Provedor de dados: 56
Autores: Teixeira,C.; Gomes,R.
Animal models have a long history of being useful tools, not only to test and select vaccines, but also to help understand the elaborate details of the immune response that follows infection. Different models have been extensively used to investigate putative immunological correlates of protection against parasitic diseases that are important to reach a successful vaccine. The greatest challenge has been the improvement and adaptation of these models to reflect the reality of human disease and the screening of vaccine candidates capable of overcoming the challenge of natural transmission. This review will discuss the advantages and challenges of using experimental animal models for vaccine development and how the knowledge achieved can be extrapolated to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human vaccines; Animal models; Malaria; Leishmaniasis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200109

Changes in the behavioral and immunological parameters of the mollusk Biomphalaria tenagophila induced by disruption of the circadian cycle as a consequence of continuous illumination
Provedor de dados: 56
Autores: Waissel,I.; Mineo,J.R.; Natal,C.L.
In the present investigation we studied some behavioral and immunological parameters of adult gastropod mollusk, Biomphalaria tenagophila, which have been reproducing for several generations under laboratory conditions. One group of gastropods was kept on a 14-h light/10-h dark cycle, corresponding to a regular circadian cycle, and another group was exposed to continuous light for 48 h. Animals were studied along (behavioral groups) or immediately after (immunological groups) 48 h of regular circadian cycle or continuous light conditions. Stopping/floating, dragging and sliding were the behavioral aspects considered (N = 20 for regular cycle; N = 20 for continuous illumination) and number of hemocytes/µl hemolymph was the immunological parameter studied (N...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Circadian cycle; Mollusk; Neuroimmunology; Stress; Behavior.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200014

Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense
Provedor de dados: 56
Autores: Nishikawa,C.Y.; Araújo,L.M.; Kadowaki,M.A.S.; Monteiro,R.A.; Steffens,M.B.R.; Pedrosa,F.O.; Souza,E.M.; Chubatsu,L.S.
Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ54 co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biological nitrogen fixation; Azospirillum brasilense; NifA protein.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200004

Participation of neuronal nitric oxide synthase in experimental neuropathic pain induced by sciatic nerve transection
Provedor de dados: 56
Autores: Chacur,M.; Matos,R.J.B.; Alves,A.S.; Rodrigues,A.C.; Gutierrez,V.; Cury,Y.; Britto,L.R.G.
Nerve injury leads to a neuropathic pain state that results from central sensitization. This phenomenom is mediated by NMDA receptors and may involve the production of nitric oxide (NO). In this study, we investigated the expression of the neuronal isoform of NO synthase (nNOS) in the spinal cord of 3-month-old male, Wistar rats after sciatic nerve transection (SNT). Our attention was focused on the dorsal part of L3-L5 segments receiving sensory inputs from the sciatic nerve. SNT resulted in the development of neuropathic pain symptoms confirmed by evaluating mechanical hyperalgesia (Randall and Selitto test) and allodynia (von Frey hair test). Control animals did not present any alteration (sham-animals). The selective inhibitor of nNOS, 7-nitroindazole...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allodynia; Neuropathic pain; Neuronal nitric oxide synthase; Peripheral neuropathy; Sciatic nerve transection; Spinal cord.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400008

Inhibitory effect of high [Mg2+] on the vasopressin-stimulated hydroosmotic permeability of the isolated perfused cortical collecting duct
Provedor de dados: 56
Autores: Falkenstein,D.; Ribeiro,C.A.L.; Figueiredo,J.F.
High magnesium concentration inhibits the effect of arginine vasopressin (AVP) on smooth muscle contraction and platelet aggregation and also influences hepatocyte AVP receptor binding. The aim of this study was to determine the role of magnesium concentration [Mg2+] in AVP-stimulated water transport in the kidney collecting duct. The effect of low and high peritubular [Mg2+] on the AVP-stimulated osmotic water permeability coefficient (Pf) was evaluated in the isolated perfused rabbit cortical collecting duct (CCD). Control tubules bathed and perfused with standard Ringer bicarbonate solution containing 1 mM Mg2+ presented a Pf of 223.9 ± 27.2 µm/s. When Mg2+ was not added to the bathing solution, an increase in the AVP-stimulated Pf to 363.1 ± 57.2 µm/s...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cortical collecting ducts; Magnesium; Arginine vasopressin; Osmotic water permeability.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800016

L-histidine enhances learning in stressed zebrafish
Provedor de dados: 56
Autores: Cofiel,L.P.V.; Mattioli,R.
The aim of the present study was to determine the effect of the histaminergic precursor L-histidine and the H3 receptor antagonist thioperamide on the learning process of zebrafish submitted or not to confinement stress. On each of the 5 consecutive days of experiment (D1, D2, D3, D4, D5), animals had to associate an interruption of the aquarium air supply with food offering. Non-stressed zebrafish received an intraperitoneal injection of 100 mg/kg L-histidine, 10 mg/kg thioperamide or saline after training. Stressed animals received drug treatment and then were submitted to confinement stress for 1 h before the learning procedure. Time to approach the feeder was measured (in seconds) and was considered to be indicative of learning. A decrease in time to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Danio rerio; Conditioning; Feed trial; Thioperamide; H3 receptor; Blood glucose.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100018

A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
Provedor de dados: 56
Autores: Devalle,S.; Niel,C.
Torque teno virus (TTV) is a circular, single-stranded DNA virus that chronically infects healthy individuals of all ages worldwide. TTV has an extreme genetic heterogeneity which is reflected in its current classification into five main phylogenetic groups (1-5). Using specific PCR assays, it has been shown that many individuals are co-infected with TTV isolates belonging to different phylogenetic groups. Here, a multiplex PCR assay was developed, using five recombinant plasmids. Each plasmid carried an insert of different size issued from a TTV isolate belonging to a different group. The assay was able to simultaneously amplify DNAs of TTV isolates belonging to all five phylogenetic groups. Multiplex PCR was then tested satisfactorily on DNAs extracted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Co-infections; Multiplex; PCR; Torque teno virus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600006

Genetic engineering of baker's and wine yeasts using formaldehyde hyperresistance-mediating plasmids
Provedor de dados: 56
Autores: Schmidt,M.; Cömer,A.; Grey,M.; Brendel,M.
Yeast multi-copy vectors carrying the formaldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker's and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO2 during fermentation, i.e., the selecting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Transformation; Hyperresistance to formaldehyde.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200004

The left ventricular contractility of the rat heart is modulated by changes in flow and a1-adrenoceptor stimulation
Provedor de dados: 56
Autores: Vassallo,P.F.; Stefanon,I.; Rossoni,L.V.; Tucci,P.J.F.; Vassallo,D.V.
Myocardial contractility depends on several mechanisms such as coronary perfusion pressure (CPP) and flow as well as on &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;1-adrenoceptor stimulation. Both effects occur during the sympathetic stimulation mediated by norepinephrine. Norepinephrine increases force development in the heart and produces vasoconstriction increasing arterial pressure and, in turn, CPP. The contribution of each of these factors to the increase in myocardial performance needs to be clarified. Thus, in the present study we used two protocols: in the first we measured mean arterial pressure, left ventricular pressure and rate of rise of left ventricular pressure development in anesthetized rats (N = 10) submitted to phenylephrine (PE)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial performance; Coronary perfusion pressure; Coronary flow; Phenylephrine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000019

CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
Provedor de dados: 56
Autores: Mudado,M.A.; Rodrigues,A.L.; Prado,V.F.; Beirão,P.S.L.; Cruz,J.S.
T-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium channel; T-type; Electrophysiology; RT-PCR; GH3 cells.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020

Distribution of small proteoglycans and glycosaminoglycans in humerus-related articular cartilage of chickens
Provedor de dados: 56
Autores: Rodrigues,E.D.; Pimentel,E.R.; Mourão,P.A.S.; Gomes,L.
The expression of components present in the cartilaginous extracellular matrix is related to development, gender, and genotype, as well as to the biomechanical properties of each type of cartilage. In the present study, we analyzed small proteoglycans and glycosaminoglycans present in different cartilages of the chicken wing after extraction with guanidine hydrochloride or papain. Quantitative analysis of glycosaminoglycans showed a larger amount in humeral cartilage (around 200 mg/g tissue) than in articular cartilage of the radius and ulna, with 138 and 80 mg/g tissue, respectively. Non-collagenous proteins isolated were predominantly from cartilage in the proximal regions of the humerus and radius. D4 fractions obtained by ultracentrifugation were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteoglycan; Fibromodulin; Decorin; Glycosaminoglycan; Cartilage; Chicken.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300009

Effect of antipyrine on the gastric emptying of liquid in rats
Provedor de dados: 56
Autores: Soares,A.C.F.; Vinagre,A.M.; Collares,E.F.
Antipyrine (At) and dipyrone (Dp) delay gastric emptying (GE) in rats. The objective of the present study was to assess the effects of intravenous (iv) and intracerebroventricular (icv) administration of At and Dp on the GE of liquid by rats. GE was assessed in male Wistar rats (5-10 in each group) 10 min after the icv or iv drug injection by measuring percent gastric retention (%GR) of a saline test meal labeled with phenol red 10 min after administration by gavage. The At iv group was significantly higher (64.4 ± 2.6%) compared to control (33.4 ± 1.5%) but did not differ from the Dp group (54.3 ± 3.8%). After icv administration of At, %GR (34.2 ± 2%) did not differ from control (32.6 ± 1.9%), but was significantly higher after Dp (54.5 ± 2.3%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dipyrone; Antipyrine; GABA B receptors; Vagus nerve.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100015

Enhanced inhibition of murine prostatic carcinoma growth by immunization with or administration of viable human umbilical vein endothelial cells and CRM197
Provedor de dados: 56
Autores: Huiyong,Zhang; Yong,Lu; Didier,Mekoo; Yu,Zhang; Jing,Fang; Rongyue,Cao; Jingjing,Liu.
Vaccination with xenogeneic and syngeneic endothelial cells is effective for inhibiting tumor growth. Nontoxic diphtheria toxin (CRM197), as an immunogen or as a speciﬁc inhibitor of heparin-binding EGF-like growth factor, has shown promising antitumor activity. Therefore, immunization with or administration of viable human umbilical vein endothelial cells (HUVECs) combined with CRM197 could have an enhanced antitumor effect. Six-week-old C57BL/6J male mice were vaccinated with viable HUVECs, 1 x 10(6) viable HUVECs combined with 100 μg CRM197, or 100 μg CRM197 alone by ip injections once a week for 4 consecutive weeks. RM-1 cells (5 x 10(5)) were inoculated by sc injection as a preventive procedure. During the therapeutic procedure, 6-week-old male...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RM-1 prostatic carcinoma; CRM197; Immunization; Human umbilical vein endothelial cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200008

Aurapten, a coumarin with growth inhibition against Leishmania major promastigotes
Provedor de dados: 56
Autores: Napolitano,H.B.; Silva,M.; Ellena,J.; Rodrigues,B.D.G.; Almeida,A.L.C.; Vieira,P.C.; Oliva,G.; Thiemann,O.H.
Several natural compounds have been identified for the treatment of leishmaniasis. Among them are some alkaloids, chalcones, lactones, tetralones, and saponins. The new compound reported here, 7-geranyloxycoumarin, called aurapten, belongs to the chemical class of the coumarins and has a molecular weight of 298.37. The compund was extracted from the Rutaceae species Esenbeckia febrifuga and was purified from a hexane extract starting from 407.7 g of dried leaves and followed by four silica gel chromatographic fractionation steps using different solvents as the mobile phase. The resulting compound (47 mg) of shows significant growth inhibition with an LD50 of 30 µM against the tropical parasite Leishmania major, which causes severe clinical manifestations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coumarin; Aurapten (7-geranyloxycoumarin); X-rays; Leishmania; Inhibitor.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200010

Rabies infection and specific effect of vaccination in mice selected for high and low immunobiological parameters
Provedor de dados: 56
Autores: Queiroz-da-Silva,L.H.; De-Franco,M.; Sant'Anna,O.A.
Innate and acquired resistance to rabies infection was investigated in mice genetically selected for high (H) or low (L) antibody responsiveness from selections I, III and IV and in mice selected for maximal (AIRmax) or minimal (AIRmin) acute inflammatory reaction. These mouse lines were infected intramuscularly with different virus dilutions and the LD50 was determined. The HIII and HIV mouse lines were more susceptible than the LIII and LIV lines and the HI line showed a discrete but higher resistance than the LI line. Analysis of the interline (H x L) F1 hybrids from selections III and IV indicated different dominance effects on the "resistant" and" susceptible" phenotypes when the route of vaccination was changed. No differences were observed between...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rabies; Antibody; Vaccination; High and low antibody responder mice.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100008

Comparison of the neurobiological effects of attribution retraining group therapy with those of selective serotonin reuptake inhibitors
Provedor de dados: 56
Autores: Wang,C.; Zhang,N.; Zhang,Y.L.; Zhang,J.; Yang,H.; Timothy,T.C.
The aim of this study was to compare the effectiveness of attribution retraining group therapy (ARGT) with selective serotonin reuptake inhibitors (SSRIs) in the treatment of major depressive disorder (MDD), generalized anxiety disorder (GAD), and obsessive-compulsive disorder (OCD). Subjects were sequentially recruited and randomized into two groups, one receiving ARGT (n = 63) and the other SSRIs (n = 66) for 8 weeks. Fifty-four ARGT outpatients with MDD (n = 19), GAD (n = 19), and OCD (n = 16) and 55 SSRI outpatients with MDD (n = 19), GAD (n = 19), and OCD (n = 17) completed the study. All subjects were assessed using the Hamilton Depression Scale and Hamilton Anxiety Scale before and after treatment. The 10-item Yale-Brown Obsessive Compulsive Scale...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attribution retraining group therapy; Selective serotonin reuptake inhibitors; Neurobiological effects; Serotonin; Cortisol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300318

Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain
Provedor de dados: 56
Autores: Cavalcante,L.A.; Garcia-Abreu,J.; Mendes,F.A.; Moura Neto,V.; Silva,L.C.F.; Onofre,G.; Weissmüller,G.; Carvalho,S.L.
Proteoglycans are abundant in the developing brain and there is much circumstantial evidence for their roles in directional neuronal movements such as cell body migration and axonal growth. We have developed an in vitro model of astrocyte cultures of the lateral and medial sectors of the embryonic mouse midbrain, that differ in their ability to support neuritic growth of young midbrain neurons, and we have searched for the role of interactive proteins and proteoglycans in this model. Neurite production in co-cultures reveals that, irrespective of the previous location of neurons in the midbrain, medial astrocytes exert an inhibitory or nonpermissive effect on neuritic growth that is correlated to a higher content of both heparan and chondroitin sulfates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astrocytes; Axonal growth; Chondroitin sulfate; Heparan sulfate; Midline; Midbrain.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800005

Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains
Provedor de dados: 56
Autores: Cavalcante,P.A.; Prata,M.M.G.; Medeiros,P.H.Q.S.; Alves da Silva,A.V.; Quetz,J.S.; Reyes,M.A.V.; Rodrigues,T.S.; Santos,A.K.S.; Ribeiro,S.A.; Veras,H.N.; Bona,M.D.; Amaral,M.S.M.G.; Rodrigues,F.A.P.; Lima,I.F.N.; Havt,A.; Lima,A.A.M.
Epithelial cell migration is an essential response to enteric pathogens such as enteropathogenic Escherichia coli (EPEC). This study aimed to investigate the effects of EPEC infection on intestinal epithelial cell migration in vitro, as well as the involvement of type III secretion system (T3SS) and Rho GTPases. Crypt intestinal epithelial cells (IEC-6) were infected with EPEC strains (E2348/69, ΔescF, and the LDI001 strain isolated from a malnourished Brazilian child) and commensal E. coli HS. Wound migration and cell death assays were performed at different time-points. Transcription and expression of Rho GTPases were evaluated using real-time PCR and western blotting. Overall, EPEC E2348/69 reduced migration and increased apoptosis and necrosis levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteropathogenic Escherichia coli; Intestinal cell migration; Type III secretion system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000602

In vitro and in vivo studies of pirarubicin-loaded SWNT for the treatment of bladder cancer
Provedor de dados: 56
Autores: Chen,Gang; He,Yunfeng; Wu,Xiaohou; Zhang,Yao; Luo,Chunli; Jing,Peng.
Intravesical chemotherapy is an important part of the treatment for superficial bladder cancer. However, the response to it is limited and its side effects are extensive. Functional single-walled carbon nanotubes (SWNT) have shown promise for tumor-targeted accumulation and low toxicity. In the present study, we performed in vivo and in vitro investigations to determine whether SWNT-based drug delivery could induce high tumor depression in rat bladder cancer and could decrease the side effects of pirarubicin (tetrahydropyranyl-adriamycin, THP). We modified SWNT with phospholipid-branched polyethylene glycol and constructed an SWNT-THP conjugate via a cleavable ester bond. The cytotoxicity of SWNT-THP against the human bladder cancer cell line BIU-87 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single-walled carbon nanotubes; Bladder cancer; Drug vehicle; THP; Intravesical chemotherapy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800012

Time course of changes in heart rate and blood pressure variability in rats with myocardial infarction
Provedor de dados: 56
Autores: Aires,R.; Pimentel,E.B.; Forechi,L.; Dantas,E.M.; Mill,J.G.
Our aim was to determine the time course of changes in autonomic balance in the acute (1 and 3 days), sub-acute (7 days) and chronic (28 days) phases of myocardial infarction (MI) in rats. Autonomic balance was assessed by temporal and spectral analyses of blood pressure variability (BPV) and heart rate variability (HRV). Pulsatile blood pressure (BP) recordings (30 min) were obtained in awake and unrestrained male Wistar rats (N = 77; 8-10 weeks old) with MI (coronary ligature) or sham operation (SO). Data are reported as means±SE. The high frequency (HF) component (n.u.) of HRV was significantly lower in MI-1- (P&lt;0.01) and MI-3-day rats (P&lt;0.05) than in their time-control groups (SO-1=68±4 vs MI-1=35.3±4.3; SO-3=71±5.8 vs MI-3=45.2±3.8), without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; Heart rate variability; Blood pressure variability; Autonomic nervous system; Heart failure.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100605

Comparison of conventional Papanicolaou cytology samples with liquid-based cervical cytology samples from women in Pernambuco, Brazil
Provedor de dados: 56
Autores: Costa,M.O.L.P.; Heráclio,S.A.; Coelho,A.V.C.; Acioly,V.L.; Souza,P.R.E.; Correia,M.T.S.
In the present study, we compared the performance of a ThinPrep cytological method with the conventional Papanicolaou test for diagnosis of cytopathological changes, with regard to unsatisfactory results achieved at the Central Public Health Laboratory of the State of Pernambuco. A population-based, cross-sectional study was performed with women aged 18 to 65 years, who spontaneously sought gynecological services in Public Health Units in the State of Pernambuco, Northeast Brazil, between April and November 2011. All patients in the study were given a standardized questionnaire on sociodemographics, sexual characteristics, reproductive practices, and habits. A total of 525 patients were assessed by the two methods (11.05% were under the age of 25 years,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Papanicolaou cytology; Liquid-based cytology; ThinPrep.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900831

Three-year follow-up study of respiratory and systemic manifestations of chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Ferrari,R.; Tanni,S.E.; Faganello,M.M.; Caram,L.M.O.; Lucheta,P.A.; Godoy,I.
Few studies show patient outcomes over time in chronic obstructive pulmonary disease (COPD). In the present study, we monitored forced expiratory volume in the first second (FEV1) and other manifestations of the disease over 3 years in 133 COPD patients (69% males, age = 65 ± 9 years, FEV1 = 59 ± 25%) evaluated at baseline. During follow-up, 15 patients (11%) died and 23 (17%) dropped out. Measurements for 95 (72%) COPD patients alive after 3 years were analyzed. FEV1, body mass index (BMI), 6-min walking distance (6MWD), Medical Research Council scale (MRC), Saint George’s Respiratory Questionnaire (SGRQ), Charlson Comorbidity index, and BODE index were obtained at baseline and after 3 years. At baseline, 17 patients (18%) presented mild, 39% moderate,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Markers of disease severity; Quality of life; Exercise capacity; BODE Index.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100007

Association of the 894G&gt;T polymorphism of the endothelial constitutive nitric oxide synthase gene with unstable angina
Provedor de dados: 56
Autores: Iturry-Yamamoto,G.R.; Moriguchi,E.H.; Zago,A.C.; Alho,C.S.; Zago,A.J.
The 894G&gt;T polymorphism of the endothelial constitutive nitric oxide synthase gene consists of the substitution of a guanine base by a thymine at the 894th nucleotide of the gene. An association of this polymorphism with acute coronary syndromes has been described, only when in combination with other polymorphisms of this gene. The aim of the present study was to search for an association between this polymorphism and unstable angina in a southern Brazilian population. In a case-control study, 156 patients (group 1 (N = 83): unstable angina, group 2 (N = 73): stable angina) were genotyped by PCR and digestion of the product. Univariate analysis demonstrated that the minimal luminal diameter and the degree of stenosis of the culprit lesion differed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Gene; Polymorphism; Unstable angina; Coronary.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400005

Effect of SX-3228, a selective ligand for the BZ1 receptor, on sleep and waking during the light-dark cycle in the rat
Provedor de dados: 56
Autores: Alvariño,F.; Monti,J.M.; Jantos,H.; Monti,D.
The effects of the benzodiazepine1 (BZ1) receptor agonist SX-3228 were studied in rats (N = 12) implanted for chronic sleep procedures. Administration of 0.5, 1.0 and 2.5 mg/kg SX-3228, sc, to rats 1 h after the beginning of the light phase of the light-dark cycle induced a significant reduction of rapid-eye-movement sleep (REMS) during the third recording hour. Moreover, slow wave sleep (SWS) was increased during the fourth recording hour after the two largest doses of the compound. Administration of 0.5, 1.0 and 2.5 mg/kg SX-3228 one hour after the beginning of the dark period of the light-dark cycle caused a significant and maintained (6-h recording period) reduction of waking (W), whereas SWS and light sleep (LS) were increased. REMS values tended to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SX-3228; Benzodiazepine1 receptor; Sleep; Waking; REM sleep; Zolpidem; Light-dark cycle.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800011

Total and segmental colonic transit time in constipated patients with Chagas’ disease without megaesophagus or megacolon
Provedor de dados: 56
Autores: Santos,S.L.; Barcelos,I.K.; Mesquita,M.A.
Manometric and pharmacological tests have shown that motor abnormalities may occur in the non-dilated colons of chagasic patients. In order to investigate the presence of abnormalities of colonic function in constipated patients with Chagas’ disease (ChC) without megaesophagus or megacolon, studies of total and segmental colonic transit time with radiopaque markers were performed on 15 ChC patients, 27 healthy volunteers and 17 patients with idiopathic constipation (IC). The values obtained for the control group were similar to those reported in the literature (total colonic time: 34.1 ± 15.6 h; right colon: 9.9 ± 7.3 h; left colon: 10.8 ± 10 h, and rectosigmoid: 12.6 ± 9.9 h). Colonic transit time data permitted us to divide both IC and ChC patients into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colonic transit; Chagas’ disease; Constipation; Radiopaque markers; American trypanosomiasis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100006

Dipyridamole increases the cytotoxicity of cisplatin in human larynx cancer cells in vitro
Provedor de dados: 56
Autores: Rodrigues,M.; Barbosa Junior,F.; Perussi,J.R.
This paper describes the effect of dipyridamole (DIP) on the cytotoxicity of cisplatin in HEp-2 human larynx cancer cells in vitro and the nature of the interaction between cisplatin and dipyridamole. Cytotoxic assays were performed to obtain the IC50 for cisplatin. The cells were treated with 0, 20, 40, 80, 120 or 200 µM cisplatin, with or without a single concentration of DIP and incubated for 60 min at 37ºC and 5% CO2 for 3 days and then counted with a hemocytometer. The accumulation of cisplatin in the cells was measured by atomic absorption and fluorescence was used to determine the membrane binding constant of DIP. In the presence of 10, 20 and 30 µM DIP, the IC50 of cisplatin was reduced by 25, 60 and 82% in HEp-2 cells. Combination index analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Dipyridamole; Synergism; Tumor cells; Cytotoxicity; IC50.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400017

Local dermal application of a compound lidocaine cream in pain management of cancer wounds
Provedor de dados: 56
Autores: Peng,L.; Zheng,H.Y.; Dai,Y.
The aim of this study was to explore the analgesic effect of local application of compound lidocaine/prilocaine cream on cancer wounds during wound care in order to reduce the amount of morphine intake or completely replace the systemic morphine administration and optimize the protocol for cancer wound pain management. All patients were enrolled with a visual analog scale (VAS) pain score ≥4. Before wound care, 60 patients were randomly divided into 2 groups of 30 each: morphine group (10 mg tablet); topical 5% compound lidocaine cream group (0.2 g/cm2). VAS scores, heart rate, and Kolcaba comfort level were recorded for the two groups 10 min before and 10, 15, 20, and 25 min after wound care and data were analyzed statistically. The means for the pain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Local; Compound lidocaine cream; Cancer wound; Pain.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100609

Comparison of blood neoangiogenesis and lymphatic vascularization in colorectal adenomas from patients with and without concomitant colorectal cancer
Provedor de dados: 56
Autores: Moreira,L.R.; Schenka,A.A.; Latuf Filho,P.; Lima,C.S.P.; Trevisan,M.A.S.; Vassallo,J.
Blood and lymphatic vessel proliferation is essential for tumor growth and progression. Most colorectal carcinomas develop from adenomas (adenoma-carcinoma sequence) in a process due to accumulation of molecular genetic alterations. About 5% of adenomatous polyps are expected to become malignant, but data on the differential angiogenic patterns of these lesions in patients with and without concomitant cancer are missing. The aim of the present study is to compare the angiogenic and lymphatic patterns of adenomatous polyps from patients with and without sporadic cancer. Thirty adenomatous polyps (15 from patients with another principal malignant lesion, and 15 from patients without cancer) were submitted to immunohistochemical staining for CD105 (marker for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiogenesis; Colorectal adenoma; Colorectal cancer; Immunohistochemistry; CD105; D2-40.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700002

The brain decade in debate: III. Neurobiology of emotion
Provedor de dados: 56
Autores: Blanchard,C.; Blanchard,R.; Fellous,J.-M.; Guimarães,F.S.; Irwin,W.; LeDoux,J.E.; McGaugh,J.L.; Rosen,J.B.; Schenberg,L.C.; Volchan,E.; Da Cunha,C.
This article is a transcription of an electronic symposium in which active researchers were invited by the Brazilian Society of Neuroscience and Behavior (SBNeC) to discuss the advances of the last decade in the neurobiology of emotion. Four basic questions were debated: 1) What are the most critical issues/questions in the neurobiology of emotion? 2) What do we know for certain about brain processes involved in emotion and what is controversial? 3) What kinds of research are needed to resolve these controversial issues? 4) What is the relationship between learning, memory and emotion? The focus was on the existence of different neural systems for different emotions and the nature of the neural coding for the emotional states. Is emotion the result of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emotion; Fear; Anxiety; Learning; Memory; Amygdala; Nucleus accumbens; Periaqueductal gray matter.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300001

Phenotype and genotype correlation of the microconversion from the CYP21A1P to the CYP21A2 gene in congenital adrenal hyperplasia
Provedor de dados: 56
Autores: Torres,N.; Mello,M.P.; Germano,C.M.R.; Elias,L.L.K.; Moreira,A.C.; Castro,M.
Deficiency of 21-hydroxylase is the most common form of congenital adrenal hyperplasia (CAH-21OH). We determined by allele-specific PCR the frequency of microconversion in the CYP21A2 gene in 50 Brazilian patients with the classical (salt wasting: SW and simple virilizing: SV) forms and nonclassical (NC) form of CAH-21OH and correlated genotype with phenotype. Genotypes were classified into three mutation groups (A, B, and C) based on the amount of enzymatic activity in in vitro studies using adrenal cells. In 94 unrelated alleles, we diagnosed 76% of the affected alleles after screening for 7 microconversions. The most frequent point mutations observed in this series were I172N (19%), V281L (18%), and IVS2,A/C&gt;G,-12 (15%). In the SW form, the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 21-Hydroxylase; Mutation; Microconversion; Genotype; Phenotype; Congenital adrenal hyperplasia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000006

Role of chemokines in promoting instability of coronary atherosclerotic plaques and the underlying molecular mechanism
Provedor de dados: 56
Autores: Zhong,Z.X.; Li,B.; Li,C.R.; Zhang,Q.F.; Liu,Z.D.; Zhang,P.F.; Gu,X.F.; Luo,H.; Li,M.J.; Luo,H.S.; Ye,G.H.; Wen,F.L.
Our aim was to investigate the role of chemokines in promoting instability of coronary atherosclerotic plaques and the underlying molecular mechanism. Coronary angiography and intravascular ultrasound (IVUS) were performed in 60 stable angina pectoris (SAP) patients and 60 unstable angina pectoris (UAP) patients. The chemotactic activity of monocytes in the 2 groups of patients was examined in Transwell chambers. High-sensitivity C-reactive protein (hs-CRP), monocyte chemoattractant protein-1 (MCP-1), regulated on activation in normal T-cell expressed and secreted (RANTES), and fractalkine in serum were examined with ELISA kits, and expression of MCP-1, RANTES, and fractalkine mRNA was examined with real-time PCR. In the SAP group, 92 plaques were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Chemokine; Plaque; MCP-1; RANTES.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200161

Are experienced and high-level race walking athletes able to match pre-programmed with executed pacing?
Provedor de dados: 56
Autores: Alves,D.L.; Cruz,R.; Lima-Silva,A.E.; Domingos,P.R.; Bertuzzi,R.; Osiecki,R.; De-Oliveira,F.R.; Lima,J.R.P.
The objective of this study was to verify the agreement between pre-programmed and executed pacing during race walking and whether level of the athletes experience and performance influenced this relationship. Twenty-nine national and international race walkers participated in this study (14 males, 24.0±7.1 years old, and 15 females, 23.3±7.3 years old). Pre-programmed pacing for 10- and 20-km official walking races was self-selected via demonstrative pacing charts prior to races, while executed pacing was analyzed by a specialist investigator via an individual plot of current velocity versus distance. There was no agreement between pre-programmed and executed pacing (P=0.674). There was no association between the ability to match the pre-programmed pace...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aerobic evaluation; Performance; Sport; Elite athlete; Physiology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600608

Functional changes of human quadriceps muscle injured by eccentric exercise
Provedor de dados: 56
Autores: Serrão,F.V.; Foerster,B.; Spada,S.; Morales,M.M.B.; Monteiro-Pedro,V.; Tannús,A.; Salvini,T.F.
The present study evaluated functional changes of quadriceps muscle after injury induced by eccentric exercise. Maximal isometric torque of quadriceps and the surface electromyography (root mean square, RMS, and median frequency, MDF) of the vastus medialis oblique (VMO) and vastus lateralis (VL) muscles were examined before, immediately after and during the first 7 days after injury. Serum creatine kinase (CK) levels and magnetic resonance imaging (MRI) were used to identify muscle injury. The subject was used as her own control and percent refers to pre-injury data. Experiments were carried out with a sedentary 23-year-old female. Injury was induced by 4 bouts of 15 maximal isokinetic eccentric contractions (angular velocity of 5º/s; range of motion from...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Isometric torque; Surface electromyography; Quadriceps muscle; Muscle injury; Eccentric exercise; Magnetic resonance imaging.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600014

Erratum for: “Gastrodin induced HO-1 and Nrf2 up-regulation to alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells through p38 MAPK phosphorylation” [Braz J Med Biol Res (2018) 51(10): e7439]
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200902

Reduced folate carrier 1 (RFC1) is associated with cleft of the lip only
Provedor de dados: 56
Autores: Vieira,A.R.; Cooper,M.E.; Marazita,M.L.; Castilla,E.E.; Orioli,I.M.
In this report, we have reanalyzed genotyping data in a collection of families from South America based on maternal origin. Genotyping analysis was performed at the Craniofacial Anomalies Research Center at the University of Iowa. These genotypes were derived from genomic DNA samples obtained from blood spots from children born with isolated orofacial clefts in 45 hospitals located in eight countries (Argentina, Bolivia, Brazil, Chile, Ecuador, Paraguay, Uruguay, and Venezuela) collaborating with ECLAMC (Latin American Collaborative Studies of Congenital Malformations) between January 1998 and December 1999. Dried blood samples were sent by regular mail to the Laboratory of Congenital Malformations, Federal University of Rio de Janeiro. Previous findings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cleft lip and palate Reduced folate carrier 1 5; 10-Methylenetetrahydrofolate reductase Folate Oral clefts Mitochondrial DNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800009

Immunocytochemical and biochemical detection of alpha-L-fucosidase in Trypanosoma cruzi
Provedor de dados: 56
Autores: Miletti,L.C.; Almeida-de-Faria,M.; Colli,W.; Alves,M.J.M.
The aim of the present study was to demonstrate the presence of alpha-L-fucosidase in Trypanosoma cruzi. Immunocytochemical and biochemical techniques were used to localize and characterize a membrane-associated, neutral-pH-optimum, alpha-L-fucosidase from the parasite. Light and electron microscopy localized the alpha-L-fucosidase specifically on the surface of the parasite and on membranes in the posterior region of the epimastigote stage. Although much less intense, labeling was also detected on the surface of trypomastigotes. At least 50% of the alpha-L-fucosidase activity was associated with epimastigote membrane solubilized with 1 M NaCl or 1% Triton X-100, suggesting that alpha-L-fucosidase is peripherally associated with membranes. The enzyme from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Trypanosomatids; Fucosidase; Fucose; Glycoproteins; Glycolipids.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500006

Th1 polarized response induced by intramuscular DNA-HSP65 immunization is preserved in experimental atherosclerosis
Provedor de dados: 56
Autores: Fonseca,D.M.; Bonato,V.L.D.; Silva,C.L.; Sartori,A.
We previously reported that a DNA vaccine constructed with the heat shock protein (HSP65) gene from Mycobacterium leprae (DNA-HSP65) was protective and also therapeutic in experimental tuberculosis. By the intramuscular route, this vaccine elicited a predominant Th1 response that was consistent with its protective efficacy against tuberculosis. It has been suggested that the immune response to Hsp60/65 may be the link between exposure to microorganisms and increased cardiovascular risk. Additionally, the high cholesterol levels found in atherosclerosis could modulate host immunity. In this context, we evaluated if an atherogenic diet could modulate the immune response induced by the DNA-HSP65 vaccine. C57BL/6 mice (4-6 animals per group) were initially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA-HSP65; Genetic vaccine; Atherosclerosis; Tuberculosis; Th1; Cholesterol.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100010

Neonatal screening for cystic fibrosis in São Paulo State, Brazil: a pilot study
Provedor de dados: 56
Autores: Rodrigues,R.; Magalhaes,P.K.R.; Fernandes,M.I.M.; Gabetta,C.S.; Ribeiro,A.F.; Pedro,K.P.; Valdetaro,F.; Santos,J.L.F.; Souza,R.M. de; Pazin Filho,A.; Maciel,L.M.Z.
Cystic fibrosis is one of the most common autosomal recessive hereditary diseases in the Caucasian population, with an incidence of 1:2000 to 1:3500 liveborns. More than 1000 mutations have been described with the most common being F508del. It has a prevalence of 23-55% within the Brazilian population. The lack of population-based studies evaluating the incidence of cystic fibrosis in São Paulo State, Brazil, and an analysis concerning the costs of implantation of a screening program motivated the present study. A total of 60,000 dried blood samples from Guthrie cards obtained from April 2005 to January 2006 for neonatal screening at 4 reference centers in São Paulo State were analyzed. The immunoreactive trypsinogen (IRT)/IRT protocol was used with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic fibrosis; Neonatal screening; Immunoreactive trypsinogen test; Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000017

Psoriasis severity and hypothalamic-pituitary-adrenal axis function: results from the CALIPSO study
Provedor de dados: 56
Autores: Brunoni,A.R.; Santos,I.S.; Sabbag,C.; Lotufo,P.A.; Benseñor,I.M.
Psoriasis is a chronic inflammatory disease that significantly impacts life quality, being associated with stress and mental disorders. We investigated whether the activity of the hypothalamic-pituitary-adrenal (HPA) axis was associated with psoriasis severity, daily life stress and anxiety, and depressive symptoms. In this ancillary study, which was part of the CALIPSO (coronary artery calcium in psoriasis) study, saliva was collected from 102 patients with psoriasis immediately upon awakening, 30, and 60 min after awakening, at 2:00 pm and at bedtime (five time points) to determine salivary cortisol levels. We used Pearson's correlation coefficient to evaluate the association of clinical and psychopathological variables with HPA activity. We found a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psoriasis; Cortisol; HPA function; Clinical study.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201102

Evidence for a modulation of neutral trehalase activity by Ca2+ and cAMP signaling pathways in Saccharomyces cerevisiae
Provedor de dados: 56
Autores: Souza,A.C.; De Mesquita,J.F.; Panek,A.D.; Silva,J.T.; Paschoalin,V.M.F.
Saccharomyces cerevisiae neutral trehalase (encoded by NTH1) is regulated by cAMP-dependent protein kinase (PKA) and by an endogenous modulator protein. A yeast strain with knockouts of CMK1 and CMK2 genes (cmk1cmk2) and its isogenic control (CMK1CMK2) were used to investigate the role of CaM kinase II in the in vitro activation of neutral trehalase during growth on glucose. In the exponential growth phase, cmk1cmk2 cells exhibited basal trehalase activity and an activation ratio by PKA very similar to that found in CMK1CMK2 cells. At diauxie, even though both cells presented comparable basal trehalase activities, cmk1cmk2 cells showed reduced activation by PKA and lower total trehalase activity when compared to CMK1CMK2 cells. To determine if CaM kinase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Trehalase; Calmodulin; CaM kinase II; CAMP-dependent protein kinase; YLR270W.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100002

Antiretroviral therapy-associated dyslipidemia in patients from a reference center in Brazil
Provedor de dados: 56
Autores: Ceccato,M.G.B.; Bonolo,P.F.; Souza Neto,A.I.; Araújo,F.S.; Freitas,M.I.F.
The aim of this study was to determine the impact of antiretroviral therapy on the lipid profile of human immunodeficiency virus (HIV) patients before and after the initiation of highly active antiretroviral therapy (HAART). This was a cross-sectional analysis of patients receiving HAART at a reference center in Belo Horizonte, Brazil, on the basis of medical records from 2002 to 2006. Patients were included if they had at least one lipid test or a clinical or laboratory diagnosis of dyslipidemia/lipodystrophy. Among the 692 patients, 620 met the eligibility criteria. The majority were males (66.5%), middle age (average 39 years), had a low educational level (60.4%), and low income (51.0%). HAART duration ranged from 11 days to 4.6 years, with a mean of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiretroviral therapy; Dyslipidemia; Lipodystrophy; Management.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100015

The role of natural killer cells in the early period of infection in murine cutaneous leishmaniasis
Provedor de dados: 56
Autores: Laurenti,M.D.; Gidlund,M.; Ura,D.M.; Sinhorini,I.L.; Corbett,C.E.P.; Goto,H.
In order to study the role of natural killer (NK) cells during the early period of Leishmania infection, BALB/c mice were selectively and permanently depleted of NK cells by injection with 90Sr and subsequently infected with Leishmania (Leishmania) amazonensis (HSJD-1 strain). 90Sr is known to selectively deplete NK cells, leaving an intact T- and B-cell compartment and preserving the ability to produce both interferon alpha and IL-2. This method of depletion has advantages when compared with depletion using anti-NK cell monoclonal antibodies because the effect is permanent and neither activates complement nor provokes massive cell death. In the present study, after one month of treatment with 90Sr, the depletion of NK cells was shown by a more than...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cutaneous leishmaniasis; NK cells; Strontium 90; Leishmania (Leishmania) amazonensis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300012

Short-lasting systemic and regional benefits of early crystalloid infusion after intravenous inoculation of dogs with live Escherichia coli
Provedor de dados: 56
Autores: Garrido,A.G.; Poli de Figueiredo,L.F.; Cruz Jr.,R.J.; Silva,E.; Rocha e Silva,M.
We investigated the systemic and regional hemodynamic effects of early crystalloid infusion in an experimental model of septic shock induced by intravenous inoculation with live Escherichia coli. Anesthetized dogs received an intravenous infusion of 1.2 x 10(10) cfu/kg live E. coli in 30 min. After 30 min of observation, they were randomized to controls (no fluids; N = 7), or fluid resuscitation with lactated Ringer's solution, 16 ml/kg (N = 7) or 32 ml/kg (N = 7) over 30 min and followed for 120 min. Cardiac index, portal blood flow, mean arterial pressure, systemic and regional oxygen-derived variables, blood lactate, and gastric PCO2 were assessed. Rapid and progressive cardiovascular deterioration with reduction in cardiac output, mean arterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac output; Escherichia coli; Gas tonometry; Portal blood flow; Septic shock.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600009

Effect of hydroxyurea on G gamma chain fetal hemoglobin synthesis by sickle-cell disease patients
Provedor de dados: 56
Autores: Teixeira,S.M.; Cortellazzi,L.C.; Grotto,H.Z.W.
Hydroxyurea is used for sickle-cell disease patients in order to increase fetal hemoglobin synthesis and consequently decrease the severity of pain episodes. Fetal hemoglobin, which is formed by gamma-globin chains A and G, is present in a constant composition throughout fetal development: about 75% of Ggamma and 25% of Agamma. In contrast, adult red cells contain about 40% of Ggamma and 60% of Agamma. In the present study, we analyzed the effect of hydroxyurea induction on the gamma chain composition of fetal hemoglobin in 31 sickle-cell disease patients treated with hydroxyurea. The control group was composed of 30 sickle-cell disease patients not treated with hydroxyurea in clinical steady state. The patients were older than 13 years and were not...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gamma chain; Fetal hemoglobin; Hydroxyurea; Sickle-cell disease.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000002

Cancer research in Brazil - stuck in second gear?
Provedor de dados: 56
Autores: Lepique,A.P.; Bonorino,C.
This article describes the main issues regarding clinical cancer research in Brazil, including both the opportunities and the hurdles. Scientists and clinicians in this field had the opportunity to talk to regulatory agencies and to the Health Ministry representative at a meeting held in the State of Rio de Janeiro, Brazil, in April 2014. Our conclusions are that we do indeed have opportunities; however, we need to move forward regarding partnerships between academia and industry, increase the availability of funding, and provide easier navigation through the regulatory processes.
Palavras-chave: Cancer research; Clinical research; Regulatory agencies; Health Ministry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200108

Effects of heme oxygenase-1 recombinant Lactococcus lactis on the intestinal barrier of hemorrhagic shock rats
Provedor de dados: 56
Autores: Gao,X.Y.; Zhou,X.F.; Wang,H.; Lv,N.; Liu,Y.; Guo,J.R.
This study aimed to investigate the effects of heme oxygenase-1 recombinant Lactococcus lactis (LL-HO-1) on the intestinal barrier of rats with hemorrhagic shock. One hundred Sprague-Dawley male rats (280–320 g) were randomly divided into healthy control group (N group) and hemorrhagic shock group (H group). Each group was subdivided into HO1t, HO2t, HO3t, PBS and LL groups in which rats were intragastrically injected with LL-HO-1 once, twice and three times, PBS and L. lactis (LL), respectively. The mortality, intestinal myeloperoxidase (MPO) activity, intestinal contents of TNF-α, IL-10 and HO-1, and intestinal Chiu's score were determined. Results showed that in N group, the HO-1 content increased after LL-HO-1 treatment, and significant difference was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heme oxygenase-1; Hemorrhagic shock; Lactococcus lactis; Intestinal barrier; Intestinal inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700601

The regulation of apoptotic cell death
Provedor de dados: 56
Autores: Amarante-Mendes,G.P.; Green,D.R.
Apoptosis is a fundamental biological phenomenon in which the death of a cell is genetically and biochemically regulated. Different molecules are involved in the regulation of the apoptotic process. Death receptors, coupled to distinct members of the caspases as well as other adapter molecules, are involved in the initiation of the stress signals (The Indictment). Members of the Bcl-2 family control at the mitochondrial level the decision between life and death (The Judgement). The effector caspases are responsible for all morphological and biochemical changes related to apoptosis including the "eat-me" signals perceived by phagocytes and neighboring cells (The Execution). Finally, apoptosis would have little biological significance without the recognition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell death; Caspase; Mitochondria; Bcl-2; Oncogene.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900001

The role of nitric oxide in reproduction
Provedor de dados: 56
Autores: McCann,S.M.; Mastronardi,C.; Walczewska,A.; Karanth,S.; Rettori,V.; Yu,W.H.
Nitric oxide (NO) plays a crucial role in reproduction at every level in the organism. In the brain, it activates the release of luteinizing hormone-releasing hormone (LHRH). The axons of the LHRH neurons project to the mating centers in the brain stem and by afferent pathways evoke the lordosis reflex in female rats. In males, there is activation of NOergic terminals that release NO in the corpora cavernosa penis to induce erection by generation of cyclic guanosine monophosphate (cGMP). NO also activates the release of LHRH which reaches the pituitary and activates the release of gonadotropins by activating neural NO synthase (nNOS) in the pituitary gland. In the gonad, NO plays an important role in inducing ovulation and in causing luteolysis, whereas in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NO and synthase; NG-monomethyl-L-arginine; Nitroprusside; NOergic neurons; FSH; LH; LHRH; FSHRF.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100007

Assessment of the progressive nature of cell damage in the pilocarpine model of epilepsy
Provedor de dados: 56
Autores: Covolan,L.; Mello,L.E.
Pilocarpine-induced (320 mg/kg, ip) status epilepticus (SE) in adult (2-3 months) male Wistar rats results in extensive neuronal damage in limbic structures. Here we investigated whether the induction of a second SE (N = 6) would generate damage and cell loss similar to that seen after a first SE (N = 9). Counts of silver-stained (indicative of cell damage) cells, using the Gallyas argyrophil III method, revealed a markedly lower neuronal injury in animals submitted to re-induction of SE compared to rats exposed to a single episode of pilocarpine-induced SE. This effect could be explained as follows: 1) the first SE removes the vulnerable cells, leaving behind resistant cells that are not affected by the second SE; 2) the first SE confers increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell injury; Status epilepticus; Epilepsy; Pilocarpine; Spontaneous seizures.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700010

Authors' reply to "Cell therapy in dilated cardiomyopathy: back to the right scientific track?"
Provedor de dados: 56
Autores: del Corsso,C.; Campos de Carvalho,A.C.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600002

Immunodominance: a new hypothesis to explain parasite escape and host/parasite equilibrium leading to the chronic phase of Chagas' disease?
Provedor de dados: 56
Autores: Rodrigues,M.M.; Alencar,B.C.G. de; Claser,C.; Tzelepis,F.
Intense immune responses are observed during human or experimental infection with the digenetic protozoan parasite Trypanosoma cruzi. The reasons why such immune responses are unable to completely eliminate the parasites are unknown. The survival of the parasite leads to a parasite-host equilibrium found during the chronic phase of chagasic infection in most individuals. Parasite persistence is recognized as the most likely cause of the chagasic chronic pathologies. Therefore, a key question in Chagas' disease is to understand how this equilibrium is established and maintained for a long period. Understanding the basis for this equilibrium may lead to new approaches to interventions that could help millions of individuals at risk for infection or who are...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chagas' disease; Trypanosoma cruzi; Immunodominance; Major histocompatibility complex.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300001

Daily birth numbers in Passo Fundo, South Brazil, 1997-1999: trends and periodicities
Provedor de dados: 56
Autores: Mikulecky,M.; Lisboa,H.R.K.
Between October 6, 1997 and April 30, 1999, 5011 births (mean: 8.76 per day) were registered in the city of Passo Fundo, South Brazil. The sequence of 572 daily birth numbers was not random (iteration test). Neyman distribution (m = ¥) showed the best fit. Clusters of days with higher birth numbers alternated with days with low numbers of births. Periodogram analysis revealed a significant periodicity of 6.98 days. The cosinor regression, testing 10 a priori supposed period lengths, found significant seasonality peaking in August-September and significantly highest birth numbers on Thursdays. Among the lunar and solar rotation cycles, the tropic lunar cycle and its 4th harmonic were most pronounced, in agreement with results concerning natality in Germany...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Births; South Brazil; Periodogram; Cosinor; Seasonality; Weekly rhythm; Multiseptans; Cosmogeophysical-biomedical parallelism; Solar and lunar cycles.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800017

Vascular changes after cardiopulmonary bypass and ischemic cardiac arrest: roles of nitric oxide synthase and cyclooxygenase
Provedor de dados: 56
Autores: Sellke,F.W.
Cardiac surgery involving ischemic arrest and extracorporeal circulation is often associated with alterations in vascular reactivity and permeability due to changes in the expression and activity of isoforms of nitric oxide synthase and cyclooxygenase. These inflammatory changes may manifest as systemic hypotension, coronary spasm or contraction, myocardial failure, and dysfunction of the lungs, gut, brain and other organs. In addition, endothelial dysfunction may increase the occurrence of late cardiac events such as graft thrombosis and myocardial infarction. These vascular changes may lead to increased mortality and morbidity and markedly lengthen the time of hospitalization and cost of cardiac surgery. Developing a better understanding of the vascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Cyclooxygenase; Heart surgery; Endothelium; Microcirculation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100004

ERKs and mitochondria-related pathways are essential for glycyrrhizic acid-mediated neuroprotection against glutamate-induced toxicity in differentiated PC12 cells
Provedor de dados: 56
Autores: Wang,D.; Guo,T.Q.; Wang,Z.Y.; Lu,J.H.; Liu,D.P.; Meng,Q.F.; Xie,J.; Zhang,X.L.; Liu,Y.; Teng,L.S.
The present study focuses on the neuroprotective effect of glycyrrhizic acid (GA, a major compound separated from Glycyrrhiza Radix, which is a crude Chinese traditional drug) against glutamate-induced cytotoxicity in differentiated PC12 (DPC12) cells. The results showed that GA treatment improved cell viability and ameliorated abnormal glutamate-induced alterations in mitochondria in DPC12 cells. GA reversed glutamate-suppressed B-cell lymphoma 2 levels, inhibited glutamate-enhanced expressions of Bax and cleaved caspase 3, and reduced cytochrome C (Cyto C) release. Exposure to glutamate strongly inhibited phosphorylation of AKT (protein kinase B) and extracellular signal-regulated kinases (ERKs); however, GA pretreatment enhanced activation of ERKs but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycyrrhizic acid; Neuroprotection; Glutamate; ERKs; Mitochondria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900773

Pharmacological study of the mechanisms involved in the vasodilator effect produced by the acute application of triiodothyronine to rat aortic rings
Provedor de dados: 56
Autores: Lozano-Cuenca,J.; López-Canales,O.A.; Aguilar-Carrasco,J.C.; Villagrana-Zesati,J.R.; López-Mayorga,R.M.; Castillo-Henkel,E.F.; López-Canales,J.S.
A relationship between thyroid hormones and the cardiovascular system has been well established in the literature. The present in vitro study aimed to investigate the mechanisms involved in the vasodilator effect produced by the acute application of 10-8–10-4 M triiodothyronine (T3) to isolated rat aortic rings. Thoracic aortic rings from 80 adult male Wistar rats were isolated and mounted in tissue chambers filled with Krebs-Henseleit bicarbonate buffer in order to analyze the influence of endothelial tissue, inhibitors and blockers on the vascular effect produced by T3. T3 induced a vasorelaxant response in phenylephrine-precontracted rat aortic rings at higher concentrations (10-4.5–10-4.0 M). This outcome was unaffected by 3.1×10-7 M glibenclamide,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triiodothyronine; Rat aorta; Vasorelaxation; NO-cGMP/PKG pathway; K+ channels.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800604

Shared control of maltose and trehalose utilization in Candida utilis
Provedor de dados: 56
Autores: Rolim,M.F.; de Araujo,P.S.; Panek,A.D.; Paschoalin,V.M.F.; Silva,J.T.
Trehalose biosynthesis and its hydrolysis have been extensively studied in yeast, but few reports have addressed the catabolism of exogenously supplied trehalose. Here we report the catabolism of exogenous trehalose by Candida utilis. In contrast to the biphasic growth in glucose, the growth of C. utilis in a mineral medium with trehalose as the sole carbon and energy source is aerobic and exhibits the Kluyver effect. Trehalose is transported into the cell by an inducible trehalose transporter (K M of 8 mM and V MAX of 1.8 µmol trehalose min-1 mg cell (dry weight)-1. The activity of the trehalose transporter is high in cells growing in media containing trehalose or maltose and very low or absent during the growth in glucose or glycerol. Similarly, total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trehalose; Trehalase; Sugar transporter; Candida utilis; Yeast.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700002

Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty
Provedor de dados: 56
Autores: Almeida,S.A.; Petenusci,S.O.; Anselmo-Franci,J.A.; Rosa-e-Silva,A.A.M.; Lamano-Carvalho,T.L.
We investigated whether chronic stress applied from prepuberty to full sexual maturity interferes with spermatogenic and androgenic testicular functions. Male Wistar rats (40 days old) were immobilized 6 h a day for 60 days. Following immobilization, plasma concentrations of corticosterone and prolactin increased 135% and 48%, respectively, while plasma luteinizing hormone and testosterone presented a significant decrease of 29% and 37%, respectively. Plasma concentration of follicle-stimulating hormone was not altered in stressed rats. Chronic stress reduced the amount of mature spermatids in the testis by 16% and the spermatozoon concentration in the cauda epididymidis by 32%. A 17% reduction in weight and a 42% decrease in DNA content were observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Spermatogenesis; LH; FSH; Testosterone; Prolactin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100013

Lack of evidence for superantigen activity of Toxoplasma gondii towards human T cells
Provedor de dados: 56
Autores: Vallochi,A.L.; Yamamoto,J.H.; Schlesinger,D.; Machado,M.A.C.; Silveira,C.; Martins,M.C.; Belfort Jr.,R.; Kalil,J.; Rizzo,L.V.
Toxoplasma gondii is an obligatory intracellular parasite whose life cycle may include man as an intermediate host. More than 500 million people are infected with this parasite worldwide. It has been previously reported that T. gondii contains a superantigen activity. The purpose of the present study was to determine if the putative superantigen activity of T. gondii would manifest towards human T cells. Peripheral blood mononuclear cells (PBMC) from individuals with no previous contact with the parasite were evaluated for proliferation as well as specific Vß expansion after exposure to Toxoplasma antigens. Likewise, PBMC from individuals with the congenital infection were evaluated for putative Vß family deletions in their T cell repertoire. We also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma; Tolerance; Superantigen; T cell receptor.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800008

The pathogenesis of tropical spastic paraparesis/human T-cell leukemia type I-associated myelopathy
Provedor de dados: 56
Autores: Casseb,J.; Penalva-de-Oliveira,A.C.
Tropical spastic paraparesis/human T-cell leukemia type I-associated myelopathy (TSP/HAM) is caused by a human T-cell leukemia virus type I (HTLV-I) after a long incubation period. TSP/HAM is characterized by a chronic progressive paraparesis with sphincter disturbances, no/mild sensory loss, the absence of spinal cord compression and seropositivity for HTLV-I antibodies. The pathogenesis of this entity is not completely known and involves a multivariable phenomenon of immune system activation against the presence of HTLV-I antigens, leading to an inflammatory process and demyelination, mainly in the thoracic spinal cord. The current hypothesis about the pathogenesis of TSP/HAM is: 1) presence of HTLV-I antigens in the lumbar spinal cord, noted by an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical spastic paraparesis; Human T-cell leukemia type I-associated myelopathy; TSP/HAM.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200001

Analysis of heart rate control to assess thermal sensitivity responses in Brazilian toads
Provedor de dados: 56
Autores: Natali,J.E.S.; Santos,B.T.; Rodrigues,V.H.; Chauí-Berlinck,J.G.
In anurans, changes in ambient temperature influence body temperature and, therefore, energy consumption. These changes ultimately affect energy supply and, consequently, heart rate (HR). Typically, anurans living in different thermal environments have different thermal sensitivities, and these cannot be distinguished by changes in HR. We hypothesized that Rhinella jimi (a toad from a xeric environment that lives in a wide range of temperatures) would have a lower thermal sensitivity regarding cardiac control than R. icterica (originally from a tropical forest environment with a more restricted range of ambient temperatures). Thermal sensitivity was assessed by comparing animals housed at 15° and 25°C. Cardiac control was estimated by heart rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anuran; Cardiac control; Heart rate complexity; Heart rate variability; Thermal physiology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100046

Variability of Vernier acuity measurements in untrained subjects of different ages
Provedor de dados: 56
Autores: Abbud,C.M.M.; Cruz,A.A.V.
The Vernier acuity of 50 normal untrained subjects (20 males and 30 females) was measured by the method of adjustment. Subjects were divided into five age ranges with 10 subjects in each age group: 5-10, 11-20, 21-30, 31-40, and 41-50 years. All subjects had normal visual acuity (20/20) and volunteered to participate in the experiment. Children were selected from a local school and adults recruited from the technical staff of the Department of Ophthalmology of the School of Medicine. Vernier acuity was higher in adults compared to children. Intraindividual variability was high and it was estimated that for most individuals of all age groups a range of 100 to 700 trials was necessary to obtain a mean with a precision of 10%. These results suggest that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vernier acuity; Variability; Age.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200011

Social disability of Brazilian mood disorder patients
Provedor de dados: 56
Autores: Tucci,A.M.; Kerr-Corrêa,F.; Dias,R.S.
Mood disorders cause many social problems, often involving family relationships. Few studies are available in the literature comparing patients with bipolar, unipolar, dysthymic, and double depressive disorders concerning these aspects. In the present study, demographic and disease data were collected using a specifically prepared questionnaire. Social adjustment was assessed using the Disability Adjustment Scale and family relationships were evaluated using the Global Assessment of Relational Functioning Scale. One hundred patients under treatment for at least 6 months were evaluated at the Psychiatric Outpatient Clinic of the Botucatu School of Medicine, UNESP. Most patients were women (82%) more than 50 (49%) years old with at least two years of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mood disorders; Depression; Bipolar; Social adjustment; Unipolar.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100017

Relationship between disease severity and quality of life in patients with chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Sanchez,F.F.; Faganello,M.M.; Tanni,S.E.; Lucheta,P.A.; Padovani,C.R.; Godoy,I.
Few studies have evaluated the relationship between Airways Questionnaire 20 (AQ20), a measure of the quality of life, scores and physiological outcomes or with systemic markers of disease in patients with chronic obstructive pulmonary disease (COPD). The aim of the present study was to investigate the relationship of forced expiratory volume in 1 s (FEV1), body mass index, fat-free mass index, 6-min walk test (6MWT) results, dyspnea sensation and peripheral oxygen saturation (SpO2) with the quality of life of COPD patients. Ninety-nine patients with COPD (mean age: 64.2 ± 9.2 years; mean FEV1: 60.4 ± 25.2% of predicted) were evaluated using spirometry, body composition measurement and the 6MWT. The baseline dyspnea index (BDI) and the Modified Medical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary disease; Chronic obstructive pulmonary disease; St. George's Respiratory Questionnaire; Airways Questionnaire 20; Respiratory system.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000005

Assessing negative priming by attended distractors in a paper-and-pencil task
Provedor de dados: 56
Autores: Rosin,F.M.
The paper-and-pencil digit-comparison task for assessing negative priming (NP) was introduced, using a referent-size-selection procedure that was demonstrated to enhance the effect. NP is indicated by slower responses to recently ignored items, and proposed within the clinical-experimental framework as a major cognitive index of active suppression of distracting information, critical to executive functioning. The digit-comparison task requires circling digits of a list with digit-asterisk pairs (a baseline measure for digit-selection), and the larger of two digits in each pair of the unrelated (with different digits in successive digit-pairs) and related lists (in which the smaller digit subsequently became a target). A total of 56 students (18-38 years)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Negative priming; Selective attention; Paper-and-pencil tasks; Aging; Executive function.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800004

Circulating levels of adiponectin and extent of coronary artery disease in patients undergoing elective coronary angiography
Provedor de dados: 56
Autores: Souza,R.A.; Alves,C.M.R.; de Oliveira,C.S.V.; Reis,A.F.; Carvalho,A.C.
Adiponectin (APN), an adipose tissue-released adipokine with demonstrated anti-inflammatory and anti-atherogenic properties, is encoded by a gene whose polymorphisms are associated with presence of coronary artery disease (CAD). Serum APN levels are inversely related with presence and complexity of CAD. Within this context, we sought to compare levels of total APN and its high molecular weight form (HMW APN) according to clinical presentation and extent of CAD in patients undergoing elective cardiac catheterization. From March 2008 to June 2010, clinical data and blood samples for APN and HMW APN measurements were collected from 415 subjects undergoing cardiac catheterization at two tertiary centers. CAD extent was estimated by the number of coronary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipose tissue; Adipokines; Adiponectin; Coronary artery disease; Atherosclerosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200604

Response to an oral calcium load in nephrolithiasis patients with fluctuating parathyroid hormone and ionized calcium levels
Provedor de dados: 56
Autores: Gomes,S.A.; Lage,A.; Lazaretti-Castro,M.; Vieira,J.G.H.; Heilberg,I.P.
the response to an oral calcium load test was assessed in 17 hypercalciuric nephrolithiasis patients who presented elevated parathyroid hormone (PTH) irrespective of the ionized calcium (sCa2+) levels. Blood samples were collected at baseline (0 min) and at 60 and 180 min after 1 g calcium load for serum PTH, total calcium, sCa2+, and 1.25(OH)2D3 determinations. According to the sCa2+ level at baseline, patients were classified as normocalcemic (N = 9) or hypercalcemic (N = 8). Six healthy subjects were also evaluated as controls. Bone mineral density was reduced in 14/17 patients. In the normocalcemic group, mean PTH levels at 0, 60 and 180 min (95 ± 76, 56 ± 40, 57 ± 45 pg/ml, respectively) did not differ from the hypercalcemic group (130 ± 75, 68 ± 35,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parathyroid hormone; Calcium; Hyperparathyroidism; Hypercalciuria; Nephrolithiasis; Bone mineral density.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900013

Anthropometric midarm measurements can detect systemic fat-free mass depletion in patients with chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Sanchez,F.F.; Faganello,M.M.; Tanni,S.E.; Lucheta,P.A.; Pelegrino,N.G.; Hasegawa,S.H.; Ribeiro,S.M.; Godoy,I.
Our objective was to determine whether anthropometric measurements of the midarm (MA) could identify subjects with whole body fat-free mass (FFM) depletion. Fifty-five patients (31% females; age: 64.6 ± 9.3 years) with mild/very severe chronic obstructive pulmonary disease (COPD), 18 smokers without COPD (39% females; age: 49.0 ± 7.3 years) and 23 never smoked controls (57% females; age: 48.2 ± 9.6 years) were evaluated. Spirometry, muscle strength and MA circumference were measured. MA muscle area was estimated by anthropometry and MA cross-sectional area by computerized tomography (CT) scan. Bioelectrical impedance was used as the reference method for FFM. MA circumference and MA muscle area correlated with FFM and biceps and triceps strength. Receiver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat-free mass depletion; Midarm anthropometry; Chronic obstructive pulmonary disease; Malnutrition; Nutritional status assessment.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500011

Use of neuron-specific enolase for assessing the severity and outcome of neurological disorders in patients
Provedor de dados: 56
Autores: Lima,J.E.; Takayanagui,O.M.; Garcia,L.V.; Leite,J.P.
Neuron-specific enolase (NSE) is a glycolytic enzyme present almost exclusively in neurons and neuroendocrine cells. NSE levels in cerebrospinal fluid (CSF) are assumed to be useful to estimate neuronal injury and clinical outcome of patients with serious clinical manifestations such as those observed in stroke, head injury, anoxic encephalopathy, encephalitis, brain metastasis, and status epilepticus. We compared levels of NSE in serum (sNSE) and in CSF (cNSE) among four groups: patients with meningitis (N = 11), patients with encephalic injuries associated with impairment of consciousness (ENC, N = 7), patients with neurocysticercosis (N = 25), and normal subjects (N = 8). Albumin was determined in serum and CSF samples, and the albumin quotient was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuronal damage; Neuron-specific enolase; Cerebrospinal fluid; Albumin quotient.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100003

A new model for the population pharmacokinetics of didanosine in healthy subjects
Provedor de dados: 56
Autores: Velasque,L.S.; Estrela,R.C.E.; Suarez-Kurtz,G.; Struchiner,C.J.
Didanosine (ddI) is a component of highly active antiretroviral therapy drug combinations, used especially in resource-limited settings and in zidovudine-resistant patients. The population pharmacokinetics of ddI was evaluated in 48 healthy volunteers enrolled in two bioequivalence studies. These data, along with a set of co-variates, were the subject of a nonlinear mixed-effect modeling analysis using the NONMEM program. A two-compartment model with first order absorption (ADVAN3 TRANS3) was fitted to the serum ddI concentration data. Final pharmacokinetic parameters, expressed as functions of the co-variates gender and creatinine clearance (CL CR), were: oral clearance (CL = 55.1 + 240 x CL CR + 16.6 L/h for males and CL = 55.1 + 240 x CL CR for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Didanosine; Pharmacokinetics; NONMEM.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100013

Effect of passive stretching on the immobilized soleus muscle fiber morphology
Provedor de dados: 56
Autores: Coutinho,E.L.; Gomes,A.R.S.; França,C.N.; Oishi,J.; Salvini,T.F.
The aim of the present study was to determine the effect of stretching applied every 3 days to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Eighteen 16-week-old Wistar rats were used and divided into three groups of 6 animals each: a) the left soleus muscle was immobilized in the shortened position for 3 weeks; b) during immobilization, the soleus was stretched for 40 min every 3 days; c) the non-immobilized soleus was only stretched. Left and right soleus muscles were examined. One portion of the soleus was frozen for histology and muscle fiber area evaluation, while the other portion was used to identify the number and length of serial sarcomeres. Immobilized muscles (group A) showed a significant decrease in weight...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skeletal muscle; Passive stretch; Muscle immobilization; Sarcomere number; Muscle atrophy; Muscle fiber area.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200011

The 9-O-acetyl GD3 gangliosides are expressed by migrating chains of subventricular zone neurons in vitro
Provedor de dados: 56
Autores: Miyakoshi,L.M.; Mendez-Otero,R.; Hedin-Pereira,C.
Neurons from the anterior subventricular zone (SVZ) of the cerebral cortex migrate tangentially to become interneurons in the olfactory bulb during development and in adult rodents. This migration was defined as neuronophilic, independent of a radial glial substrate. The cortical SVZ and the rostral migratory stream to the olfactory bulb were shown to be rich in 9-O-acetyl GD3 gangliosides (9-O-acGD3), which have been previously shown to be implicated in gliophilic migration in the rodent cerebral cortex and cerebellum. In the present study, we performed SVZ explant cultures using rats during their first postnatal week to analyze the expression of these gangliosides in chain migration of neuronal precursors. We characterized migrating chains of these...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Subventricular zone; Migration; 9-O-acetylated gangliosides; Rostral migratory stream.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500016

Therapy of tacrolimus combined with corticosteroids in idiopathic membranous nephropathy
Provedor de dados: 56
Autores: Cui,W.; Lu,X.; Min,X.; Liu,M.; Guan,S.; Wang,Y.; Luo,M.; Li,W.; Li,Q.; Dong,W.; Miao,L.; Luo,P.
We evaluated the efficacy and safety of tacrolimus (TAC) combined with corticosteroids in treating patients with idiopathic membranous nephropathy (IMN). One hundred seventy-seven biopsy-proven IMN patients were recruited in this retrospective clinical study. Sixty patients received TAC (target blood concentration of 4–8 ng/mL) and 117 patients received daily cyclophosphamide (CYC, 100 mg) combined with prednisone. Remission rates at the end of the first, second and third month in the TAC group were significantly higher than that in the CYC group (1st: 35.0 vs 19.7%, P&lt;0.05; 2nd: 56.7 vs 38.5%, P&lt;0.05; 3rd: 76.7 vs 59.0%, P&lt;0.05). In the first 3 months, daily urinary protein and serum albumin in the TAC group obtained a better improvement than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclophosphamide; Efficacy; Idiopathic membranous nephropathy; Retrospective clinical study; Tacrolimus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400703

Diversity among satellite glial cells in dorsal root ganglia of the rat
Provedor de dados: 56
Autores: Nascimento,R.S.; Santiago,M.F.; Marques,S.A.; Allodi,S.; Martinez,A.M.B.
Peripheral glial cells consist of satellite, enteric glial, and Schwann cells. In dorsal root ganglia, besides pseudo-unipolar neurons, myelinated and nonmyelinated fibers, macrophages, and fibroblasts, satellite cells also constitute the resident components. Information on satellite cells is not abundant; however, they appear to provide mechanical and metabolic support for neurons by forming an envelope surrounding their cell bodies. Although there is a heterogeneous population of neurons in the dorsal root ganglia, satellite cells have been described to be a homogeneous group of perineuronal cells. Our objective was to characterize the ultrastructure, immunohistochemistry, and histochemistry of the satellite cells of the dorsal root ganglia of 17 adult...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Satellite glial cells; Peripheral nervous system; Vimentin; S-100; Electron microscopy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100011

Role of Notch signaling in the mammalian heart
Provedor de dados: 56
Autores: Zhou,X.L.; Liu,J.C.
Notch signaling is an evolutionarily ancient, highly conserved pathway important for deciding cell fate, cellular development, differentiation, proliferation, apoptosis, adhesion, and epithelial-to-mesenchymal transition. Notch signaling is also critical in mammalian cardiogenesis, as mutations in this signaling pathway are linked to human congenital heart disease. Furthermore, Notch signaling can repair myocardial injury by promoting myocardial regeneration, protecting ischemic myocardium, inducing angiogenesis, and negatively regulating cardiac fibroblast-myofibroblast transformation. This review provides an update on the known roles of Notch signaling in the mammalian heart. The goal is to assist in developing strategies to influence Notch signaling and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Notch signaling; Congenital heart disease; Myocardial regeneration; Cardioprotection; Angiogenesis; Cardiac fibroblast-myofibroblast transformation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100001

Calcium antagonism and the vasorelaxation of the rat aorta induced by rotundifolone
Provedor de dados: 56
Autores: Guedes,D.N.; Silva,D.F.; Barbosa-Filho,J.M.; Medeiros,I.A.
The vasorelaxing activity of rotundifolone (ROT), a major constituent (63.5%) of the essential oil of Mentha x villosa, was tested in male Wistar rats (300-350 g). In isolated rat aortic rings, increasing ROT concentrations (0.3, 1, 10, 100, 300, and 500 µg/ml) inhibited the contractile effects of 1 µM phenylephrine and of 80 or 30 mM KCl (IC50 values, reported as means ± SEM = 184 ± 6, 185 ± 3 and 188 ± 19 µg/ml, N = 6, respectively). In aortic rings pre-contracted with 1 µM phenylephrine, the smooth muscle-relaxant activity of ROT was inhibited by removal of the vascular endothelium (IC50 value = 235 ± 7 µg/ml, N = 6). Furthermore, ROT inhibited (pD2 = 6.04, N = 6) the CaCl2-induced contraction in depolarizing medium in a concentration-dependent manner....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+ mobilization; Rat aortic rings; Rotundifolone; Vasorelaxation; Mentha x villosa.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200014

Fetal hemoglobin levels are related to metabolic control in diabetic subjects
Provedor de dados: 56
Autores: Pardini,V.C.; Victória,I.M.N.; Pieroni,F.B.; Milagres,G.; Nascimento,P.D.; Velho,G.; Purisch,S.; Pardini,H.
We have investigated the relationship between fetal hemoglobin (HbF) levels and metabolic control in subjects with insulin-dependent (N = 79) and non-insulin-dependent diabetes mellitus (N = 242). HbF and hemoglobin A1c (HbA1c) levels were increased in subjects with type 1 and type 2 diabetes as compared to levels in nondiabetic individuals (P&amp;lt;0.0001), and were significantly higher in type 1 than in type 2 diabetes subjects. Lower levels of HbA1c and HbF were observed in type 2 diabetes subjects treated by diet, intermediate levels in those treated with oral hypoglycemic agents, and higher levels in those treated with insulin. HbF and HbA1c levels were correlated in type 1 diabetes (R2 = 0.57, P&amp;lt;0.0001) and type 2 diabetes (R2 = 0.58,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fetal hemoglobin; HbA1c; Glycated hemoglobin; Diabetes mellitus; Type 1 diabetes; Type 2 diabetes.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600005

Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil
Provedor de dados: 56
Autores: Pisa,G.; Magnani,G.S.; Weber,H.; Souza,E.M.; Faoro,H.; Monteiro,R.A.; Daros,E.; Baura,V.; Bespalhok,J.P.; Pedrosa,F.O.; Cruz,L.M.
Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire bacterial communities in the environment. In the present study, we have used this approach to assess the bacterial community in the rhizosphere soil of sugarcane at different times and under different nitrogen fertilization conditions. At the high taxonomic level, few...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 16S rRNA; Biodiversity; Nitrogen fixation; Bacteria; Sugarcane; Soil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200004

Evaluation of cardiovascular toxicity of carbon nanotubes functionalized with sodium hyaluronate in oral regenerative medicine
Provedor de dados: 56
Autores: Joviano-Santos,J.V.; Sá,M.A.; De Maria,M.L.A.; Almeida,T.C.S.; Geraldo,V.; Oliveira,S.; Ladeira,L.O.; Ferreira,A.J.
It has been demonstrated that carbon nanotubes (CNTs) associated with sodium hyaluronate (HY-CNTs) accelerate bone repair in the tooth sockets of rats. Before clinical application of HY-CNTs, it is important to assess their biocompatibility. Moreover, cardiac toxicity may be caused by the translocation of these particles to the blood stream. The aim of this study was to evaluate possible changes in cardiovascular function in male Wistar rats whose tooth sockets were treated with either CNTs or HY-CNTs (100 μg/mL, 0.1 mL). Blood pressure and heart rate were monitored in conscious rats 7 days after treatment. Cardiac function was evaluated using the Langendorff perfusion technique. The data showed no changes in blood pressure or heart rate in rats treated...
Palavras-chave: Cardiac function; Blood pressure; Carbon nanotubes; Nanotechnology; Bone repair; Tooth sockets.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700560

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA
Provedor de dados: 56
Autores: Lima Barros,A.E.; Almeida,A.M.P.; Carvalho Jr.,L.B.; Azevedo,W.M.
We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Polysiloxane; Polyvinyl alcohol; Solid phase; Immunodetection; Yersinia pestis; ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008

Synthesis and secretion of transferrin by a bovine trabecular meshwork cell line
Provedor de dados: 56
Autores: Bertazolli-Filho,R.; Laicine,E.M.; Haddad,A.
The trabecular meshwork (TM) is the main outflow pathway in the mammalian eye. Oxidative damage to TM cells has been suggested to be an important cause of impairment of TM functions, leading to deficient drainage of aqueous humor, with deleterious consequences to the eye. Transferrin, a metalloprotein involved in iron transport, has been characterized as an intrinsic eye protein. Since transferrin is implicated in the control of oxidative stress, the objective of the present study was to determine if a bovine TM cell line (CTOB) synthesizes and secretes transferrin. The CTOB cell line was cultured in the presence of 35S-methionine and the incubation medium was submitted to immunoprecipitation. Total RNAs from CTOB and isolated bovine TM (freshly isolated,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine eye; Trabecular meshwork; Transferrin expression; Cell and tissue culture; Immunocytochemistry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000007

A phthalide derivative isolated from endophytic fungiPestalotiopsis photiniae induces G1 cell cycle arrest and apoptosis in human HeLa cells
Provedor de dados: 56
Autores: Chen,C.; Yang,R.L.
MP [4-(3′,3′-dimethylallyloxy)-5-methyl-6-methoxyphthalide] was obtained from liquid culture of Pestalotiopsis photiniaeisolated from the Chinese Podocarpaceae plant Podocarpus macrophyllus. MP significantly inhibited the proliferation of HeLa tumor cell lines. After treatment with MP, characteristic apoptotic features such as DNA fragmentation and chromatin condensation were observed in DAPI-stained HeLa cells. Flow cytometry showed that MP induced G1 cell cycle arrest and apoptosis in a dose-dependent manner. Western blotting and real-time reverse transcription-polymerase chain reaction were used to investigate protein and mRNA expression. MP caused significant cell cycle arrest by upregulating the cyclin-dependent kinase inhibitor p27KIP1 protein and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MP [4-(3′; 3′-dimethylallyloxy)-5-methyl-6-methoxyphthalide] p73 Apoptosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800643

Maternal serum progesterone, estradiol and estriol levels in successful dinoprostone-induced labor
Provedor de dados: 56
Autores: Konopka,C.K.; Morais,E.N.; Naidon,D.; Pereira,A.M.; Rubin,M.A.; Oliveira,J.F.; Mello,C.F.
Hormone-mediated quiescence involves the maintenance of a decreased inflammatory responsiveness. However, no study has investigated whether labor induction with prostanoids is associated with changes in the levels of maternal serum hormones. The objective of this study was to determine whether labor induction with dinoprostone is associated with changes in maternal serum progesterone, estradiol, and estriol levels. Blood samples were obtained from 81 pregnant women at term. Sixteen patients had vaginal birth after spontaneous labor, 12 required cesarean section after spontaneous labor and 16 underwent elective cesarean. Thirty-seven patients had labor induction with dinoprostone. Eligible patients received a vaginal insert of dinoprostone (10 mg) and were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Labor induction Dinoprostone; PGE2 Progesterone Estriol Estradiol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100091

Diversity of genomic electropherotypes of naturally occurring equine herpesvirus 1 isolates in Argentina
Provedor de dados: 56
Autores: Galosi,C.M.; Norimine,J.; Echeverría,M.G.; Oliva,G.A.; Nosetto,E.O.; Etcheverrigaray,M.E.; Tohya,Y.; Mikami,T.
The genomes of 10 equine herpesvirus 1 (EHV-1) strains isolated in Argentina from 1979 to 1991, and a Japanese HH1 reference strain were compared by restriction endonuclease analysis. Two restriction enzymes, BamHI and BglII, were used and analysis of the electropherotypes did not show significant differences among isolates obtained from horses with different clinical signs. This suggests that the EHV-1 isolates studied, which circulated in Argentina for more than 10 years, belong to a single genotype.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Argentina; Electropherotypes; Equine herpesvirus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600007

Quenching of the intrinsic fluorescence of bovine serum albumin by chlorpromazine and hemin
Provedor de dados: 56
Autores: Silva,D.; Cortez,C.M.; Louro,S.R.W.
The binding of chlorpromazine (CPZ) and hemin to bovine serum albumin was studied by the fluorescence quenching technique. CPZ is a widely used anti-psychotic drug that interacts with blood components, influences bioavailability, and affects function of several biomolecules. Hemin is an important ferric residue of hemoglobin that binds within the hydrophobic region of albumin with high specificity. Quenching of the intrinsic fluorescence of bovine serum albumin (BSA) was observed by selectively exciting tryptophan residues at 290 nm. Emission spectra were recorded in the range from 300 to 450 nm for each quencher addition. Stern-Volmer graphs were plotted, and the quenching constant estimated for BSA solution titrated with hemin at 25ºC was 1.44 (± 0.05) x...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chlorpromazine; Hemin; Albumin; Fluorescence quenching.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700004

Heterozygous HTRA1 missense mutation in CADASIL-like family disease
Provedor de dados: 56
Autores: Wu,Xiaowei; Li,Changxin; Mao,Jinming; Li,Ling; Liu,Yan; Hou,Yao.
The aim of this study was to find related pathogenic genes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy in (CADASIL)-like patients. The direct sequencing and high-throughput multiplex polymerase chain reaction (PCR) was performed to screen for related genes. The clinical and imaging data of a CADASIL-like patient (the pro-band) and his family members were collected. At first, the known hereditary cerebral vascular genes of the pro-band were screened with direct sequencing to find candidate gene mutations. High-throughput multiplex PCR was then used to analyze the single nucleotide polymorphism of the candidate gene in the family members. The results showed that there was missense mutation of the high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTRA1; CADASIL-like; SNP; Cerebral small vessel disease.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500602

Effect of cyclooxygenase inhibitors on gentamicin-induced nephrotoxicity in rats
Provedor de dados: 56
Autores: Hosaka,E.M.; Santos,O.F.P.; Seguro,A.C.; Vattimo,M.F.F.
The frequent use of nonsteroidal anti-inflammatory drugs (NSAID) in combination with gentamicin poses the additional risk of nephrotoxic renal failure. Cyclooxygenase-1 (COX-1) is the main enzyme responsible for the synthesis of renal vasodilator prostaglandins, while COX-2 participates predominantly in the inflammatory process. Both are inhibited by non-selective NSAID such as indomethacin. Selective COX-2 inhibitors such as rofecoxib seem to have fewer renal side effects than non-selective inhibitors. The objective of the present study was to determine whether the combined use of rofecoxib and gentamicin can prevent the increased renal injury caused by gentamicin and indomethacin. Male Wistar rats (250-300 g) were treated with gentamicin (100 mg/kg body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indomethacin; Rofecoxib; Nonsteroidal anti-inflammatory drugs; Aminoglycoside; Acute renal failure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700006

Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus
Provedor de dados: 56
Autores: Ruiz,F.S.; Andersen,M.L.; Zager,A.; Martins,R.C.S.; Tufik,S.
The objective of the present study was to determine whether sleep deprivation (SD) would promote changes in lymphocyte numbers in a type 1 diabetes model (non-obese diabetic, NOD, mouse strain) and to determine whether SD would affect female and male NOD compared to Swiss mice. The number of lymphocytes in peripheral blood after 24 and 96 h of SD (by multiple platform method) or equivalent period of time in home-cage controls was examined prior to the onset of diabetes. SD for 96 h significantly reduced lymphocytes in male Swiss mice compared to control (8.6 ± 2.1 vs 4.1 ± 0.7 10³/µL; P &lt; 0.02). In male NOD animals, 24- and 96-h SD caused a significant decrease of lymphocytes compared to control (4.4 ± 0.3 vs 1.6 ± 0.5; P &lt; 0.001 and 4.4 ± 0.3 vs 0.9...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sleep deprivation; Stress; Lymphopenia; Diabetes; Non-obese diabetic mouse; Swiss mice.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500005

Predictive significance of standardized uptake value parameters of FDG-PET in patients with non-small cell lung carcinoma
Provedor de dados: 56
Autores: Duan,X-Y.; Wang,W.; Li,M.; Li,Y.; Guo,Y-M.
18F-fluoro-2-deoxyglucose (FDG) positron emission tomography (PET)/computed tomography (CT) is widely used to diagnose and stage non-small cell lung cancer (NSCLC). The aim of this retrospective study was to evaluate the predictive ability of different FDG standardized uptake values (SUVs) in 74 patients with newly diagnosed NSCLC. 18F-FDG PET/CT scans were performed and different SUV parameters (SUVmax, SUVavg, SUVT/L, and SUVT/A) obtained, and their relationship with clinical characteristics were investigated. Meanwhile, correlation and multiple stepwise regression analyses were performed to determine the primary predictor of SUVs for NSCLC. Age, gender, and tumor size significantly affected SUV parameters. The mean SUVs of squamous cell carcinoma were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 18F-FDG PET/CT; Standardized uptake value (SUV); Prediction; Non-small cell lung cancer.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300267

Short-term levosimendan treatment protects rat testes against oxidative stress
Provedor de dados: 56
Autores: Yuksel,M.B.; Kavak,S.; Gecit,I.; Basel,H.; Gümrükçüoğlu,H.A.; Demir,H.; Meral,İ.
The objective of this study was to evaluate the effect of short-term levosimendan exposure on oxidant/antioxidant status and trace element levels in the testes of rats under physiological conditions. Twenty male Wistar albino rats were randomly divided into two groups of 10 animals each. Group 1 was not exposed to levosimendan and served as control. Levosimendan (12 µg/kg) diluted in 10 mL 0.9% NaCl was administered intraperitoneally to group 2. Animals of both groups were sacrificed after 3 days and their testes were harvested for the determination of changes in tissue oxidant/antioxidant status and trace element levels. Tissue malondialdehyde (MDA) was significantly lower in the levosimendan group (P &lt; 0.001) than in the untreated control group and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Levosimendan; Oxidative stress; Reactive oxygen species; Rat testes; SOD; GSH-Px; MDA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800005

Acute effect of alcohol intake on sine-wave Cartesian and polar contrast sensitivity functions
Provedor de dados: 56
Autores: Cavalcanti-Galdino,M.K.; da Silva,J.A.; Mendes,L.C.; dos Santos,N.A.; Simas,M.L.B.
The aim of this study was to assess contrast sensitivity for angular frequency stimuli as well as for sine-wave gratings in adults under the effect of acute ingestion of alcohol. We measured the contrast sensitivity function (CSF) for gratings of 0.25, 1.25, 2.5, 4, 10, and 20 cycles per degree of visual angle (cpd) as well as for angular frequency stimuli of 1, 2, 4, 24, 48, and 96 cycles/360°. Twenty adults free of ocular diseases, with normal or corrected-to-normal visual acuity, and no history of alcoholism were enrolled in two experimental groups: 1) no alcohol intake (control group) and 2) alcohol ingestion (experimental group). The average concentration of alcohol in the experimental group was set to about 0.08%. We used a paradigm involving a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visual perception; Contrast sensitivity function; Alcohol intake; Angular frequency; Psychophysical method.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400321

Prenatal management, pregnancy and pediatric outcomes in fetuses with septated cystic hygroma
Provedor de dados: 56
Autores: Sanhal,C.Y.; Mendilcioglu,I.; Ozekinci,M.; Yakut,S.; Merdun,Z.; Simsek,M.; Luleci,G.
It has been reported that, compared with simple increased nuchal translucency, fetal cases with septated cystic hygroma (CH) are more likely to face perinatal handicaps. However, pediatric outcomes and proper prenatal counseling for this anomaly have not yet been truly defined. We performed this study to determine pregnancy and pediatric outcomes of fetuses with septated CH. We searched records for cases with septated CH and collected data for structural abnormalities, karyotype analysis, and pregnancy outcomes. Fetuses born with septated CH were also evaluated for their pediatric outcomes. Sixty-nine fetuses with septated CH were enrolled in the study. Results showed that chromosomal abnormalities were present in 28 fetuses (40.6%), and the most common...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Septated; Cystic; Hygroma; Perinatal; Outcomes; Neurologic.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900799

Effect of chloride dialysate concentration on metabolic acidosis in aintenance hemodialysis patients
Provedor de dados: 56
Autores: Marques,F.O.; Libório,A.B.; Daher,E.F.
Hyperchloremia is one of the multiple etiologies of metabolic acidosis in hemodialysis (HD) patients. The aim of the present study was to determine the influence of chloride dialysate on metabolic acidosis control in this population. We enrolled 30 patients in maintenance HD program with a standard base excess (SBE) ≤2 mEq/L and urine output of less than 100 mL/24 h. The patients underwent dialysis three times per week with a chloride dialysate concentration of 111 mEq/L for 4 weeks, and thereafter with a chloride dialysate concentration of 107 mEq/L for the next 4 weeks. Arterial blood was drawn immediately before the second dialysis session of the week at the end of each phase, and the Stewart physicochemical approach was applied. The strong ion gap...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chloride dialysate; Hemodialysis; Metabolic acidosis; Stewart model.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000012

Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil
Provedor de dados: 56
Autores: Kerbauy,G.; Perugini,M.R.E.; Yamauchi,L.M.; Yamada-Ogatta,S.F.
In this study, we report the characterization of a strain of Enterococcus faecium vanA, which grows only in the presence of vancomycin (VDEfm-UEL). The bacterium was isolated from the feces of a female patient who had undergone surgical treatment of Reinke’s edema and was receiving intravenous vancomycin therapy for infection with methicillin/oxacillin-resistant Staphylococcus aureus, a postoperative complication. Antimicrobial dependence was further confirmed by the vancomycin E-test. VDEfm-UEL was also shown to be resistant to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, levofloxacin, penicillin, rifampicin, and teicoplanin. The putative virulence genes efaA, gelE and esp were detected by PCR. The ddl gene from VDEfm-UEL was cloned and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Enterococcus faecium; Vancomycin; Dependence; VanA genotype; Virulence factors.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300013

Purification and binding analysis of the nitrogen fixation regulatory NifA protein from Azospirillum brasilense
Provedor de dados: 56
Autores: Passaglia,L.M.P.; Van Soom,C.; Schrank,A.; Schrank,I.S.
NifA protein activates transcription of nitrogen fixation operons by the alternative sigma54 holoenzyme form of RNA polymerase. This protein binds to a well-defined upstream activator sequence (UAS) located at the -200/-100 position of nif promoters with the consensus motif TGT-N10-ACA. NifA of Azospirillum brasilense was purified in the form of a glutathione-S-transferase (GST)-NifA fusion protein and proteolytic release of GST yielded inactive and partially soluble NifA. However, the purified NifA was able to induce the production of specific anti-A. brasilense NifA-antiserum that recognized NifA from A. brasilense but not from K. pneumoniae. Both GST-NifA and NifA expressed from the E. coli tac promoter are able to activate transcription from the nifHDK...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NifA protein; NifA antibodies; Azospirillum brasilense; Gel shift assay; NifA activity; Upstream activator sequence.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100001

Assessment of ventilatory neuromuscular drive in patients with obstructive sleep apnea
Provedor de dados: 56
Autores: Bittencourt,L.R.A.; Moura,S.M.T.; Bagnato,M.C.; Gregório,L.C.; Tufik,S.; Nery,L.E.
The presence of abnormalities of the respiratory center in obstructive sleep apnea (OSA) patients and their correlation with polysomnographic data are still a matter of controversy. Moderately obese, sleep-deprived OSA patients presenting daytime hypersomnolence, with normocapnia and no clinical or spirometric evidence of pulmonary disease, were selected. We assessed the ventilatory control and correlated it with polysomnographic data. Ventilatory neuromuscular drive was evaluated in these patients by measuring the ventilatory response (VE), the inspiratory occlusion pressure (P.1) and the ventilatory pattern (VT/TI, TI/TTOT) at rest and during submaximal exercise, breathing room air. These analyses were also performed after inhalation of a hypercapnic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ventilatory neuromuscular drive; Sleep apnea; Polysomnography; Ventilatory control.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400005

Origin and pathogenesis of antiphospholipid antibodies
Provedor de dados: 56
Autores: Celli,C.M.; Gharavi,A.E.
Antiphospholipid antibodies (aPL) are a heterogeneous group of antibodies that are detected in the serum of patients with a variety of conditions, including autoimmune (systemic lupus erythematosus), infectious (syphilis, AIDS) and lymphoproliferative disorders (paraproteinemia, myeloma, lymphocytic leukemias). Thrombosis, thrombocytopenia, recurrent fetal loss and other clinical complications are currently associated with a subgroup of aPL designating the antiphospholipid syndrome. In contrast, aPL from patients with infectious disorders are not associated with any clinical manifestation. These findings led to increased interest in the origin and pathogenesis of aPL. Here we present the clinical features of the antiphospholipid syndrome and review the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiphospholipid antibodies; Antiphospholipid syndrome; Systemic lupus erythematosus; Syphilis; Cardiolipin; Beta-2-glycoprotein I.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600002

Use of magnitude-squared coherence to identify the maximum driving response band of the somatosensory evoked potential
Provedor de dados: 56
Autores: Infantosi,A.F.C.; Melges,D.B.; Tierra-Criollo,C.J.
The present study proposes to apply magnitude-squared coherence (MSC) to the somatosensory evoked potential for identifying the maximum driving response band. EEG signals, leads [Fpz'-Cz'] and [C3'-C4'], were collected from two groups of normal volunteers, stimulated at the rate of 4.91 (G1: 26 volunteers) and 5.13 Hz (G2: 18 volunteers). About 1400 stimuli were applied to the right tibial nerve at the motor threshold level. After applying the anti-aliasing filter, the signals were digitized and then further low-pass filtered (200 Hz, 6th order Butterworth and zero-phase). Based on the rejection of the null hypothesis of response absence (MSC(f) &gt; 0.0060 with 500 epochs and the level of significance set at a = 0.05), the beta and gamma bands, 15-66 Hz,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma oscillations; Somatosensory response detection; Tibial nerve; Magnitude-squared coherence.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200011

Participation of ATP-sensitive K+ channels in the peripheral antinociceptive effect of fentanyl in rats
Provedor de dados: 56
Autores: Rodrigues,A.R.A.; Castro,M.S.A.; Francischi,J.N.; Perez,A.C.; Duarte,I.D.G.
We examined the effect of several K+ channel blockers such as glibenclamide, tolbutamide, charybdotoxin (ChTX), apamin, tetraethylammonium chloride (TEA), 4-aminopyridine (4-AP), and cesium on the ability of fentanyl, a clinically used selective µ-opioid receptor agonist, to promote peripheral antinociception. Antinociception was measured by the paw pressure test in male Wistar rats weighing 180-250 g (N = 5 animals per group). Carrageenan (250 µg/paw) decreased the threshold of responsiveness to noxious pressure (delta = 188.1 ± 5.3 g). This mechanical hyperalgesia was reduced by fentanyl (0.5, 1.5 and 3 µg/paw) in a peripherally mediated and dose-dependent fashion (17.3, 45.3 and 62.6%, respectively). The selective blockers of ATP-sensitive K+ channels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peripheral antinociception; Fentanyl; K+ channel; Μ-Opioid receptor agonist; Glibenclamide.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100014

Translation, adaptation and validation of the Roland-Morris questionnaire - Brazil Roland-Morris
Provedor de dados: 56
Autores: Nusbaum,L.; Natour,J.; Ferraz,M.B.; Goldenberg,J.
The purpose of the present study was to translate the Roland-Morris (RM) questionnaire into Brazilian-Portuguese and adapt and validate it. First 3 English teachers independently translated the original questionnaire into Brazilian-Portuguese and a consensus version was generated. Later, 3 other translators, blind to the original questionnaire, performed a back translation. This version was then compared with the original English questionnaire. Discrepancies were discussed and solved by a panel of 3 rheumatologists and the final Brazilian version was established (Brazil-RM). This version was then pretested on 30 chronic low back pain patients consecutively selected from the spine disorders outpatient clinic. In addition to the traditional clinical outcome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low back pain; Disability; Roland-Morris questionnaire; Spine.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200007

Restless leg syndrome, sleep quality and fatigue in multiple sclerosis patients
Provedor de dados: 56
Autores: Moreira,N.C.V.; Damasceno,R.S.; Medeiros,C.A.M.; de Bruin,P.F.C.; Teixeira,C.A.C.; Horta,W.G.; de Bruin,V.M.S.
We have tested the hypothesis that restless leg syndrome (RLS) is related to quality of sleep, fatigue and clinical disability in multiple sclerosis (MS). The diagnosis of RLS used the four minimum criteria defined by the International Restless Legs Syndrome Study Group. Fatigue was assessed by the Fatigue Severity Scale (FSS &gt;27), quality of sleep by the Pittsburgh Sleep Quality Index (PSQI &gt;6), excessive daytime sleepiness by the Epworth Sleepiness Scale (ESS &gt;10) and clinical disability by the Expanded Disability Status Scale (EDSS). Forty-four patients (32 women) aged 14 to 64 years (43 ± 14) with disease from 0.4 to 23 years (6.7 ± 5.9) were evaluated. Thirty-five were classified as relapsing-remitting, 5 as primary progressive and 4 as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restless leg syndrome; Multiple sclerosis; Sleep; Fatigue; Pittsburgh Sleep Quality Index.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000017

Induction of interleukin-10 by HIV antigens in peripheral mononuclear cells of health care workers after occupational exposure to HIV-1-positive blood
Provedor de dados: 56
Autores: Rodrigues,D.S.S.; Brunialti,M.K.C.; Medeiros,E.A.S.; Diaz,R.S.; Turcato,G.; Salomao,R.
Evaluation of HIV-induced IL-2 production by peripheral blood mononuclear cells (PBMC) and HIV-specific T helper and cytotoxic T lymphocyte (CTL) responses in health care workers (HCW) occupationally exposed to HIV reveals a high rate of response to HIV among non-seroconverters. IL-10 is also known to interfere with HIV infection in vitro. To evaluate the induction of IL-10 by HIV antigens in HCW occupationally exposed to HIV, 18 HCW with percutaneous injury were enrolled in this study, 9 of them exposed to HIV-contaminated blood, and 9 exposed to HIV-negative blood. PBMC were incubated on plates coated with HIV-1 antigens, and IL-10 was measured in supernatants by ELISA. Five of nine HCW exposed to HIV-contaminated blood presented HIV-induced IL-10. Two...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV-1; Interleukin-10; Needlestick injury; Occupational exposure; Health care worker.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600010

The effect of an aerobic training program on the electrical remodeling of the heart: high-frequency components of the signal-averaged electrocardiogram are predictors of the maximal aerobic power
Provedor de dados: 56
Autores: Marocolo,M.; Nadal,J.; Benchimol-Barbosa,P.R.
Increased heart rate variability (HRV) and high-frequency content of the terminal region of the ventricular activation of signal-averaged ECG (SAECG) have been reported in athletes. The present study investigates HRV and SAECG parameters as predictors of maximal aerobic power (VO2max) in athletes. HRV, SAECG and VO2max were determined in 18 high-performance long-distance (25 ± 6 years; 17 males) runners 24 h after a training session. Clinical visits, ECG and VO2max determination were scheduled for all athletes during thew training period. A group of 18 untrained healthy volunteers matched for age, gender, and body surface area was included as controls. SAECG was acquired in the resting supine position for 15 min and processed to extract average RR interval...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Long-distance runners; Signal-averaged electrocardiogram; Maximal aerobic power; Mathematical model.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200006

Extracellular matrix molecules play diverse roles in the growth and guidance of central nervous system axons
Provedor de dados: 56
Autores: Pires-Neto,M.A.; Braga-de-Souza,S.; Lent,R.
Axon growth and guidance represent complex biological processes in which probably intervene diverse sets of molecular cues that allow for the appropriate wiring of the central nervous system (CNS). The extracellular matrix (ECM) represents a major contributor of molecular signals either diffusible or membrane-bound that may regulate different stages of neural development. Some of the brain ECM molecules form tridimensional structures (tunnels and boundaries) that appear during time- and space-regulated events, possibly playing relevant roles in the control of axon elongation and pathfinding. This short review focuses mainly on the recognized roles played by proteoglycans, laminin, fibronectin and tenascin in axonal development during ontogenesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Axon guidance; Development; Anterior commissure; Corpus callosum; Brain.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500017

Effects of estragole on the compound action potential of the rat sciatic nerve
Provedor de dados: 56
Autores: Leal-Cardoso,J.H.; Matos-Brito,B.G.; Lopes-Junior,J.E.G.; Viana-Cardoso,K.V.; Sampaio-Freitas,A.B.; Brasil,R.O.; Coelho-de-Souza,A.N.; Albuquerque,A.A.C.
Estragole, a relatively nontoxic terpenoid ether, is an important constituent of many essential oils with widespread applications in folk medicine and aromatherapy and known to have potent local anesthetic activity. We investigated the effects of estragole on the compound action potential (CAP) of the rat sciatic nerve. The experiments were carried out on sciatic nerves dissected from Wistar rats. Nerves, mounted in a moist chamber, were stimulated at a frequency of 0.2 Hz, with electric pulses of 50-100-µs duration at 10-20 V, and evoked CAP were monitored on an oscilloscope and recorded on a computer. CAP control parameters were: peak-to-peak amplitude (PPA), 9.9 ± 0.55 mV (N = 15), conduction velocity, 92.2 ± 4.36 m/s (N = 15), chronaxy, 45.6 ± 3.74 µs...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estragole; Essential oil; Sciatic nerve; Compound action potential; Nerve conduction velocity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800009

Comparison of the efficacy of biodegradable and non-biodegradable scintillation liquids on the counting of tritium- and [14C]-labeled compounds
Provedor de dados: 56
Autores: Medeiros,R.B.; Godinho,R.O.; Mattos,M.F.S.S.
The widespread use of ³H and 14C in research has generated a large volume of waste mixed with scintillation liquid, requiring an effective control and appropriate storage of liquid radioactive waste. In the present study, we compared the efficacy of three commercially available scintillation liquids, Optiphase HiSafe 3, Ultima-Gold™ AB (biodegradable) and Insta-Gel-XF (non-biodegradable), in terms of [14C]-glucose and [³H]-thymidine counting efficiency. We also analyzed the effect of the relative amount of water (1.6 to 50%), radioisotope concentration (0.1 to 100 nCi/ml), pH (2 to 10) and color of the solutions (samples containing 0.1 to 1.0 mg/ml of Trypan blue) on the counting efficiency in the presence of these scintillation liquids. There were few...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradable chemicals; Scintillation liquids; Liquid waste; Color quenching; Scintillation counting efficiency; Tritium and 14C counting.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200016

Motor behavioral abnormalities and histopathological findings of Wistar rats inoculated with HTLV-1-infected MT2 cells
Provedor de dados: 56
Autores: Câmara,C.C.; Oriá,R.B.; Felismino,T.C.; Silva,A.P. da; Silva,M.A.M. da; Alcântara,J.V.A.; Costa,S.B.C.; Vicente,A.C.P.; Teixeira-Santos,T.J.; Castro-Costa,C.M. de.
The objective of the present study was to describe motor behavioral changes in association with histopathological and hematological findings in Wistar rats inoculated intravenously with human T-cell lymphotropic virus type 1 (HTLV-1)-infected MT2 cells. Twenty-five 4-month-old male rats were inoculated with HTLV-1-infected MT2 cells and 13 control rats were inoculated with normal human lymphocytes. The behavior of the rats was observed before and 5, 10, 15, and 20 months after inoculation during a 30-min/rat testing time for 5 consecutive days. During each of 4 periods, a subset of rats was randomly chosen to be sacrificed in order to harvest the spinal cord for histopathological analysis and to obtain blood for serological and molecular studies....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Motor behaviors; HTLV-1-infected MT2 cells; Wistar rats; Experimental myelopathy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700008

Estrous cycle and stress: influence of progesterone on the female brain
Provedor de dados: 56
Autores: Lovick,T.A.
The female brain operates in a constantly changing chemical milieu caused by cyclical changes in gonadal hormones during the estrous cycle (menstrual cycle in women). Such hormones are highly lipophilic and pass readily from the plasma to the brain where they can influence neuronal function. It is becoming clear that the rapid reduction in peripheral circulating progesterone, which occurs during the late diestrous phase of the cycle, can trigger a withdrawal-like response, in which changes in GABA A receptor expression render hyper-responsive certain brain areas involved in processing responses to stressful stimuli. The periaqueductal gray matter (PAG) is recognised as an important region for integrating anxiety/defence responses. Withdrawal from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Female; Estrous cycle; Stress; Anxiety; Progesterone; Neurosteroid replacement.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400005

Involvement of β3-adrenergic receptors in the control of food intake in rats
Provedor de dados: 56
Autores: Kanzler,S.A.; Januario,A.C.; Paschoalini,M.A.
This study examined the food intake changes evoked by intracerebroventricular (icv) injection of a selective agonist (BRL37344, 2 and 20 nmol) or antagonist (SR59230A, 10 and 50 nmol) of β3-adrenergic receptors in 24-h fasted rats (adult male Wistar rats, 200-350 g, N = 6/treatment). The animals were also pretreated with saline icv (SAL) or SR59230A (50 nmol) followed by BRL37344 (20 nmol) or SAL in order to determine the selectivity of the effects evoked by BRL37344 on food intake or the selectivity of the effects evoked by SR59230A on risk assessment (RA) behavior. The highest dose of BRL37344 (N = 7) decreased food intake 1 h after the treatment (6.4 ± 0.5 g in SAL-treated vs 4.2 ± 0.8 g in drug-treated rats). While both doses of SR59230A failed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food intake CNS Intracerebroventricular injection; Β3-adrenergic receptor.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100010

Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis
Provedor de dados: 56
Autores: Bedendo,J.; Pignatari,A.C.C.
Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from São Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular typing; Enterococcus faecium; PCR-PFGE.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100002

Enhancement of declarative memory associated with emotional content in a Brazilian sample
Provedor de dados: 56
Autores: Frank,J.E.; Tomaz,C.
Several studies have documented that emotional arousal may enhance long-term memory. This is an adaptation of a paradigm previously used in North American and European samples in investigations of the influence of emotion on long-term retention. A sample of 46 healthy adults of high and low educational levels watched a slide presentation of stories. A randomly assigned group watched a story with an arousing content and another group watched a neutral story. The stories were matched for structure and comprehensibility and the set and order of the 11 slides were the same in both conditions. Immediately after viewing the slide presentation, the participants were asked to rate the emotionality of the narrative. The arousing narrative was rated as being more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory; Emotion; Stories; Brazilian.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200013

How frequently should a patient taking amiodarone be screened for thyroid dysfunction?
Provedor de dados: 56
Autores: Pazin-Filho,A.; de Jesus,A.M.X.; Magalhães,P.K.R.; Melato,L.H.; Campos,D.; Maciel,B.C.; Maciel,L.M.Z.
Amiodarone-induced thyroid dysfunction (AITD) is a common complication of amiodarone therapy and its prevalence varies according to iodine intake, subclinical thyroid disorders and the definition of AITD. There is no consensus about the frequency of screening for this condition. We evaluated 121 patients on chronic regular intake of amiodarone (mean intake = 248.5 ± 89 mg; duration of treatment = 5.3 ± 3.9 years, range = 0.57-17 years) and with stable baseline cardiac condition. Those with no AITD were followed up for a median period of 3.2 years (range: 0.6-6.7) and the incidence rate of AITD, defined by clinical and laboratorial findings as proposed by international guidelines, was obtained (62.8 per 1000 patients/year). We applied the Cox proportional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amiodarone; Chagas’ disease; Thyroid dysfunction; Antiarrhythmic drug.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800009

Thymocyte migration: an affair of multiple cellular interactions?
Provedor de dados: 56
Autores: Savino,W.; Ayres Martins,S.; Neves-dos-Santos,S.; Smaniotto,S.; Ocampo,J.S.P.; Mendes-da-Cruz,D.A.; Terra-Granado,E.; Kusmenok,O.; Villa-Verde,D.M.S.
Cell migration is a crucial event in the general process of thymocyte differentiation. The cellular interactions involved in the control of this migration are beginning to be defined. At least chemokines and extracellular matrix proteins appear to be part of the game. Cells of the thymic microenvironment produce these two groups of molecules, whereas developing thymocytes express the corresponding receptors. Moreover, although chemokines and extracellular matrix can drive thymocyte migration per se, a combined role for these molecules appears to contribute to the resulting migration patterns of thymocytes in their various stages of differentiation. The dynamics of chemokine and extracellular matrix production and degradation is not yet well understood....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thymocyte migration; Extracellular matrix; Integrins; Chemokines; Thymic epithelial cells; Thymic nurse cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800007

Tea polyphenols induce S phase arrest and apoptosis in gallbladder cancer cells
Provedor de dados: 56
Autores: Wang,Jiaqi; Pan,Yixuan; Hu,Jiacheng; Ma,Qiang; Xu,Yi; Zhang,Yijian; Zhang,Fei; Liu,Yingbin.
Gallbladder cancer (GBC) is the most common malignancy in the biliary tract. Without effective treatment, its prognosis is notoriously poor. Tea polyphenols (TPs) have many pharmacological and health benefits, including antioxidant, anti-inflammatory, anti-tumor, anti-thrombotic, antibacterial, and vasodilatory properties. However, the anti-cancer effect of TPs in human gallbladder cancer has not yet been determined. Cell viability and colony formation assay were used to investigate the cell growth. Cell cycle and apoptosis were evaluated by flow cytometry analysis. Western blot assay was used to detect the expression of proteins related to cell cycle and apoptosis. Human tumor xenografts were used to examine the effect of TPs on gallbladder cancer cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallbladder cancer; Tea polyphenols; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400614

Comparative efficacy and safety of the left versus right radial approach for percutaneous coronary procedures: a meta-analysis including 6870 patients
Provedor de dados: 56
Autores: Xia,S.L.; Zhang,X.B.; Zhou,J.S.; Gao,X.
The radial approach is widely used in the treatment of patients with coronary artery disease. We conducted a meta-analysis of published results on the efficacy and safety of the left and right radial approaches in patients undergoing percutaneous coronary procedures. A systematic search of reference databases was conducted, and data from 14 randomized controlled trials involving 6870 participants were analyzed. The left radial approach was associated with significant reductions in fluoroscopy time [standardized mean difference (SMD)=-0.14, 95% confidence interval (CI)=-0.19 to -0.09; P&lt;0.00001] and contrast volume (SMD=-0.07, 95%CI=-0.12 to -0.02; P=0.009). There were no significant differences in rate of procedural failure of the left and the right...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radial approach; Percutaneous coronary; Meta-analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800743

IGF2, LEPR, POMC, PPARG, and PPARGC1 gene variants are associated with obesity-related risk phenotypes in Brazilian children and adolescents
Provedor de dados: 56
Autores: Queiroz,E.M.; Cândido,A.P.C.; Castro,I.M.; Bastos,A.Q.A.; Machado-Coelho,G.L.L.; Freitas,R.N.
Association studies of genetic variants and obesity and/or obesity-related risk factors have yielded contradictory results. The aim of the present study was to determine the possible association of five single-nucleotide polymorphisms (SNPs) located in the IGF2, LEPR, POMC, PPARG, and PPARGC1 genes with obesity or obesity-related risk phenotypes. This case-control study assessed overweight (n=192) and normal-weight (n=211) children and adolescents. The SNPs were analyzed using minisequencing assays, and variables and genotype distributions between the groups were compared using one-way analysis of variance and Pearson's chi-square or Fisher's exact tests. Logistic regression analysis adjusted for age and gender was used to calculate the odds ratios (ORs)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Association study; Obesity; Genetic polymorphisms; Brazilian population.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700595

Hormonal therapy with estradiol and drospirenone improves endothelium-dependent vasodilation in the coronary bed of ovariectomized spontaneously hypertensive rats
Provedor de dados: 56
Autores: Borgo,M.V.; Claudio,E.R.G.; Silva,F.B.; Romero,W.G.; Gouvea,S.A.; Moysés,M.R.; Santos,R.L.; Almeida,S.A.; Podratz,P.L.; Graceli,J.B.; Abreu,G.R.
Drospirenone (DRSP) is a progestin with anti-aldosterone properties and it reduces blood pressure in hypertensive women. However, the effects of DRSP on endothelium-dependent coronary vasodilation have not been evaluated. This study investigated the effects of combined therapy with estrogen (E2) and DRSP on endothelium-dependent vasodilation of the coronary bed of ovariectomized (OVX) spontaneously hypertensive rats. Female spontaneously hypertensive rats (n=87) at 12 weeks of age were randomly divided into sham operated (Sham), OVX, OVX treated with E2 (E2), and OVX treated with E2 and DRSP (E2+DRSP) groups. Hemodynamic parameters were directly evaluated by catheter insertion into the femoral artery. Endothelium-dependent vasodilation in response to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Menopause; Hypertension; Hormone therapy; Drospirenone; Coronary reactivity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100601

Specific structural features of syndecans and heparan sulfate chains are needed for cell signaling
Provedor de dados: 56
Autores: Lopes,C.C.; Dietrich,C.P.; Nader,H.B.
The syndecans, heparan sulfate proteoglycans, are abundant molecules associated with the cell surface and extracellular matrix and consist of a protein core to which heparan sulfate chains are covalently attached. Each of the syndecan core proteins has a short cytoplasmic domain that binds cytosolic regulatory factors. The syndecans also contain highly conserved transmembrane domains and extracellular domains for which important activities are becoming known. These protein domains locate the syndecan on cell surface sites during development and tumor formation where they interact with other receptors to regulate signaling and cytoskeletal organization. The functions of cell surface heparan sulfate proteoglycan have been centered on the role of heparan...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Syndecans; Heparan sulfate proteoglycans; Protein kinase C; Growth factors; Cell signaling.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200001

Effect of Walker 256 tumor growth on intestinal absorption of leucine, methionine and glucose in newly weaned and mature rats
Provedor de dados: 56
Autores: Gomes-Marcondes,M.C.C.; Honma,H.N.; Areas,M.A.; Cury,L.
In tumor-bearing rats, most of the serum amino acids are used for synthesis and oxidation processes by the neoplastic tissue. In the present study, the effect of Walker 256 carcinoma growth on the intestinal absorption of leucine, methionine and glucose was investigated in newly weaned and mature rats. Food intake and carcass weight were decreased in newly weaned (NT) and mature (MT) rats bearing Walker 256 tumor in comparison with control animals (NC and MC). The tumor/carcass weight ratio was higher in NT than in MT rats, whereas nitrogen balance was significantly decreased in both as compared to control animals. Glucose absorption was significantly reduced in MT rats (MT = 47.3 ± 4.9 vs MC = 99.8 ± 5.3 nmol min-1 cm-1, Kruskal-Wallis test,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Walker 256 tumor; Intestinal absorption; Amino acids; Glucose; Aging.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000017

The anti-IRBP IgG1 and IgG2a response does not correlate with susceptibility to experimental autoimmune uveitis
Provedor de dados: 56
Autores: Vieira de Moraes,L.; Martins,G.A.; Flangini,M.; Ibañez,O.M.; Sant'Anna,O.A.; Rizzo,L.V.
Susceptibility to experimental autoimmune uveitis (EAU) in inbred mice has been associated with a dominant Th1 response. Elevated anti-inter-photoreceptor retinoid-binding protein (anti-IRBP) IgG2a/IgG1 antibody ratios have been implicated as candidate markers to predict disease severity. In the present study, both the anti-IRBP antibody isotype and severity of EAU phenotypes were examined in 4 non-isogenic genetically selected mouse lines to determine if they can be used as general markers of disease. Mice between 8 and 12 weeks old selected for high (H III) or low (L III) antibody response and for maximum (AIR MAX) or minimum (AIR MIN) acute inflammatory reaction (AIR) were immunized with IRBP. Each experiment was performed with at least 5 mice per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoimmunity; IgG1 and IgG2a isotypes; Inflammation; Genetically selected mice; Uveitis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600010

Effects of vacuum sealing drainage on the treatment of cranial bone-exposed wounds in rabbits
Provedor de dados: 56
Autores: Chen,X.J.; Liu,S.; Gao,G.Z.; Yan,D.X.; Jiang,W.S.
This study was designed to assess the efficacy of vacuum sealing drainage (VSD) on skull exposure wounds in rabbits and to investigate the underlying mechanism of the process. Full-thickness excisional circular wounds 2×2 cm with or without periosteum involvement were created in 88 New Zealand white rabbits (mean body weight: 3.0±0.65 kg). Animals were randomly divided into 4 groups: periosteum-intact wounds treated with traditional dressing (p+control), periosteum-intact wounds treated with VSD (p+VSD), periosteum-lacking wounds treated with traditional dressing (p–control) and periosteum-lacking wounds treated with VSD (p–VSD). The wounds treated with traditional dressing were covered with Vaseline gauze, while VSD treatment was accompanied with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cranial bone-exposed wound; Vacuum sealing drainage; Periosteum; Wound healing; Efficacy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200602

Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in Brazilian elderly
Provedor de dados: 56
Autores: Simonsen,V.; Brandão,A.P.; Brandileone,M.C.C.; Yara,T.I.; Di Fabio,J.L.; Lopes,M.H.; Jacob Filho,W.
Serum antibodies specific for the capsular polysaccharides of Streptococcus pneumoniae provide protection against invasive pneumococcal infection. In Brazil, this vaccine has been used for people over 65 years with clinical risk to develop pneumococcal infection since 1999. We evaluated the immune response of 102 elderly subjects (75.5% females and 24.5% males) with a mean age of 71 years, and 19 young healthy adults (63.2% females and 36.8% males) with a mean age of 27 years. The elderly study group consisted of outpatients who received follow-up care in the Geriatric Department of General Hospital, Faculty of Medicine, University of São Paulo. None had acute illness at the time of vaccination. Both groups were immunized with one intra-deltoid injection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Pneumococcal polysaccharide vaccine; Immune response; Elderly subjects.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200014

Antiphospholipid antibodies in Brazilian hepatitis C virus carriers
Provedor de dados: 56
Autores: Atta,A.M.; Estevam,P.; Paraná,R.; Pereira,C.M.; Leite,B.C.O.; Sousa-Atta,M.L.B.
Hepatitis C, a worldwide viral infection, is an important health problem in Brazil. The virus causes chronic infection, provoking B lymphocyte dysfunction, as represented by cryoglobulinemia, non-organ-specific autoantibody production, and non-Hodgkin's lymphoma. The aim of this research was to screen for the presence of antiphospholipid autoantibodies in 109 Brazilian hepatitis C virus carriers without clinical history of antiphospholipid syndrome. Forty healthy individuals were used as the control group. IgA, IgG, and IgM antibodies against cardiolipin and β2-glycoprotein I were measured with an enzyme-linked immunosorbent assay, using a cut-off point of either 20 UPL or 20 SBU. While 24 (22.0%) hepatitis C carriers had moderate titers of IgM...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis C virus; Antiphospholipid antibody; Brazilian patients.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600009

Clinical features of patients with type 2 diabetes mellitus and hepatitis C infection
Provedor de dados: 56
Autores: Greca,L.F.; Pinto,L.C.; Rados,D.R.; Canani,L.H.; Gross,J.L.
The objective of the present cross-sectional study was to assess the prevalence and the clinical and laboratory features of hepatitis C virus (HCV)-positive patients with type 2 diabetes mellitus (DM) attending either an outpatient clinic or hemodialysis units. Serologic-HCV testing was performed in 489 type 2 DM patients (303 outpatients and 186 on dialysis). A structured assessment of clinical, laboratory and DM-related complications was performed and the patients were then compared according to HCV infection status. Mean patient age was 60 years; HCV positivity (HCV+) was observed in 39 of 303 (12.9%) outpatients and in 34 of 186 (18.7%) dialysis patients. Among HCV+ patients, 32 were men (43.8%). HCV+ patients had higher serum levels of aspartate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes; Hepatitis C; Dialysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300015

Sloth biology: an update on their physiological ecology, behavior and role as vectors of arthropods and arboviruses
Provedor de dados: 56
Autores: Gilmore,D.P.; Da Costa,C.P.; Duarte,D.P.F.
This is a review of the research undertaken since 1971 on the behavior and physiological ecology of sloths. The animals exhibit numerous fascinating features. Sloth hair is extremely specialized for a wet tropical environment and contains symbiotic algae. Activity shows circadian and seasonal variation. Nutrients derived from the food, particularly in Bradypus, only barely match the requirements for energy expenditure. Sloths are hosts to a fascinating array of commensal and parasitic arthropods and are carriers of various arthropod-borne viruses. Sloths are known reservoirs of the flagellate protozoan which causes leishmaniasis in humans, and may also carry trypanosomes and the protozoan Pneumocystis carinii.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sloths; Ecology; Behavior; Parasites; Bradypus; Choloepus.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100002

Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800)
Provedor de dados: 56
Autores: Artico,L.O.; Bianchini,A.; Grubel,K.S.; Monteiro,D.S.; Estima,S.C.; de Oliveira,L.R.; Bonatto,S.L.; Marins,L.F.
The South American sea lion, Otaria flavescens, is widely distributed along the Pacific and Atlantic coasts of South America. However, along the Brazilian coast, there are only two nonbreeding sites for the species (Refúgio de Vida Silvestre da Ilha dos Lobos and Refúgio de Vida Silvestre do Molhe Leste da Barra do Rio Grande), both in Southern Brazil. In this region, the species is continuously under the effect of anthropic activities, mainly those related to environmental contamination with organic and inorganic chemicals and fishery interactions. This paper reports, for the first time, the genetic diversity of O. flavescens found along the Southern Brazilian coast. A 287-bp fragment of the mitochondrial DNA control region (D-loop) was analyzed. Seven...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Otaria flavescens; MtDNA; D-loop; Genetic variability; New haplotypes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900002

Body composition measures of obese adolescents by the deuterium oxide dilution method and by bioelectrical impedance
Provedor de dados: 56
Autores: Resende,C.M.M.; Camelo Júnior,J.S.; Vieira,M.N.C.M.; Ferriolli,E.; Pfrimer,K.; Perdoná,G.S.C.; Monteiro,J.P.
The objectives of the present study were to describe and compare the body composition variables determined by bioelectrical impedance (BIA) and the deuterium dilution method (DDM), to identify possible correlations and agreement between the two methods, and to construct a linear regression model including anthropometric measures. Obese adolescents were evaluated by anthropometric measures, and body composition was assessed by BIA and DDM. Forty obese adolescents were included in the study. Comparison of the mean values for the following variables: fat body mass (FM; kg), fat-free mass (FFM; kg), and total body water (TBW; %) determined by DDM and by BIA revealed significant differences. BIA overestimated FFM and TBW and underestimated FM. When compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adolescents; Obesity; Body composition; Bioelectrical impedance; Deuterium oxide dilution method.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100013

Lipoprotein(a) as a predictor of poor collateral circulation in patients with chronic stable coronary heart disease
Provedor de dados: 56
Autores: Fan,Y.; Hu,J.-S.; Guo,F.; Lu,Z.-B.; Xia,H.
As a mechanism compensating for obstructive coronary artery disease, coronary collateral circulation (CCC) has attracted cardiologists for a long time to explore its potential impact. In the present study, Chinese patients suffering from ≥95% coronary stenosis, as diagnosed by angiography, have been investigated for the correlation between CCC and lipoprotein(a) [Lp(a)] levels. A cohort of 654 patients was divided into four categories according to Rentrop grades 0, 1, 2, and 3. Lp(a) levels were divided into model 1, discretized with critical values of 33 and 66%, and model 2, discretized with a cutoff value of 30.0 mg/dL. Furthermore, we evaluated the correlation between CCC and serum Lp(a) levels. The four groups had significantly different Lp(a) levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipoprotein(a); Coronary collateral circulation; Predictor.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800606

Topographic distribution of the tibial somatosensory evoked potential using coherence
Provedor de dados: 56
Autores: Melges,D.B.; Infantosi,A.F.C.; Miranda de Sá,A.M.F.L.
The objective of the present study was to determine the adequate cortical regions based on the signal-to-noise ratio (SNR) for somatosensory evoked potential (SEP) recording. This investigation was carried out using magnitude-squared coherence (MSC), a frequency domain objective response detection technique. Electroencephalographic signals were collected (International 10-20 System) from 38 volunteers, without history of neurological pathology, during somatosensory stimulation. Stimuli were applied to the right posterior tibial nerve at the rate of 5 Hz and intensity slightly above the motor threshold. Response detection was based on rejecting the null hypothesis of response absence (significance level α= 0.05 and M = 500 epochs). The best detection rates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatosensory response detection; Magnitude-squared coherence; Tibial nerve; Topography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200004

Cyanobacterial toxins in Portugal: effects on aquatic animals and risk for human health
Provedor de dados: 56
Autores: Vasconcelos,V.M.
Toxic cyanobacteria are common in Portuguese freshwaters and the most common toxins are microcystins. The occurrence of microcystin-LR (MCYST-LR) has been reported since 1990 and a significant number of water reservoirs that are used for drinking water attain high levels of this toxin. Aquatic animals that live in eutrophic freshwater ecosystems may be killed by microcystins but in many cases the toxicity is sublethal and so the animals can survive long enough to accumulate the toxins and transfer them along the food chain. Among these, edible mollusks, fish and crayfish are especially important because they are harvested and sold for human consumption. Mussels that live in estuarine waters and rivers where toxic blooms occur may accumulate toxins without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyanobacteria; Toxins; Risk; Human health; Microcystins; Accumulation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300001

Engagement of multifocal neural circuits during recall of autobiographical happy events
Provedor de dados: 56
Autores: Cerqueira,C.T.; Almeida,J.R.C.; Gorenstein,C.; Gentil,V.; Leite,C.C.; Sato,J.R.; Amaro Jr.,E.; Busatto,G.F.
Happy emotional states have not been extensively explored in functional magnetic resonance imaging studies using autobiographic recall paradigms. We investigated the brain circuitry engaged during induction of happiness by standardized script-driven autobiographical recall in 11 healthy subjects (6 males), aged 32.4 ± 7.2 years, without physical or psychiatric disorders, selected according to their ability to vividly recall personal experiences. Blood oxygen level-dependent (BOLD) changes were recorded during auditory presentation of personal scripts of happiness, neutral content and negative emotional content (irritability). The same uniform structure was used for the cueing narratives of both emotionally salient and neutral conditions, in order to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional magnetic resonance imaging; Autobiographical recall; Emotion induction; Affective states.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200006

Possible mechanisms involved in the vasorelaxant effect produced by clobenzorex in aortic segments of rats
Provedor de dados: 56
Autores: Lozano-Cuenca,J.; González-Hernández,A.; López-Canales,O.A.; Villagrana-Zesati,J.R.; Rodríguez-Choreão,J.D.; Morín-Zaragoza,R.; Castillo-Henkel,E.F.; López-Canales,J.S.
Clobenzorex is a metabolic precursor of amphetamine indicated for the treatment of obesity. Amphetamines have been involved with cardiovascular side effects such as hypertension and pulmonary arterial hypertension. The aim of the present study was to investigate whether the direct application of 10–9–10–5 M clobenzorex on isolated phenylephrine-precontracted rat aortic rings produces vascular effects, and if so, what mechanisms may be involved. Clobenzorex produced an immediate concentration-dependent vasorelaxant effect at the higher concentrations (10–7.5–10–5 M). The present outcome was not modified by 10–6 M atropine (an antagonist of muscarinic acetylcholine receptors), 3.1×10–7 M glibenclamide (an ATP-sensitive K+ channel blocker), 10–3 M...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clobenzorex; Rat aorta; Vasorelaxation; NO-cGMP; PKG pathway; K+ channels.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900605

Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
Provedor de dados: 56
Autores: Uchoa,A.F.; Souza,P.A.S.; Zarate,R.M.L.; Gomes-Filho,E.; Campos,F.A.P.
We describe here the isolation and characterization of a major albumin from the seeds of Opuntia ficus-indica (Cactaceae). This protein has a molecular mass of 6.5 kDa and was isolated by a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of this protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The N-terminal amino acid sequence of this protein is Asp-Pro-Tyr-Trp-Glu-Gln-Arg.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactaceae; Prickly-pear; Opuntia ficus-indica; Seed proteins; 2S albumins.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600005

Induction of apoptosis in cancer cells by tumor necrosis factor and butyrolactone, an inhibitor of cyclin-dependent kinases
Provedor de dados: 56
Autores: Belizário,J.E.; Sherwood,S.; Beçak,W.
Induction of apoptosis by tumor necrosis factor (TNF) is modulated by changes in the expression and activity of several cell cycle regulatory proteins. We examined the effects of TNF (1-100 ng/ml) and butyrolactone I (100 µM), a specific inhibitor of cyclin-dependent kinases (CDK) with high selectivity for CDK-1 and CDK-2, on three different cancer cell lines: WEHI, L929 and HeLa S3. Both compounds blocked cell growth, but only TNF induced the common events of apoptosis, i.e., chromatin condensation and ladder pattern of DNA fragmentation in these cell lines. The TNF-induced apoptosis events were increased in the presence of butyrolactone. In vitro phosphorylation assays for exogenous histone H1 and endogenous retinoblastoma protein (pRb) in the total cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell cycle; Cyclin-dependent kinases; Cyclin-dependent kinase chemical inhibitors; Tumor necrosis factor.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400016

Assessment of indicators of vitamin A status in non-cirrhotic chronic hepatitis C patients
Provedor de dados: 56
Autores: Santana,R.C.; Machado,A.A.; Martinelli,A.L.C.; Jordão,A.A.; Ramalho,L.N.Z.; Vannucchi,H.
Subjects with chronic liver disease are susceptible to hypovitaminosis A due to several factors. Therefore, identifying patients with vitamin deficiency and a requirement for vitamin supplementation is important. Most studies assessing vitamin A in the context of hepatic disorders are conducted using cirrhotic patients. A cross-sectional study was conducted in 43 non-cirrhotic patients with chronic hepatitis C to evaluate markers of vitamin A status represented by serum retinol, liver retinol, and serum retinol-binding protein levels. We also performed the relative dose-response test, which provides an indirect estimate of hepatic vitamin A reserves. These vitamin A indicators were assessed according to the stage of liver fibrosis using the METAVIR score...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin A; Hepatitis C; Retinol; Retinol binding protein; Liver fibrosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100702

The role of IFN-gamma and IL-4 in gastric mucosa inflammation associated with Helicobacter heilmannii type 1 infection
Provedor de dados: 56
Autores: Cinque,S.M.S.; Rocha,G.A.; Correa-Oliveira,R.; Soares,T.F.; Moura,S.B.; Rocha,A.M.C.; Nogueira,A.M.M.F.; Cabral,M.M.D.A.; Vieira,L.Q.; Martins-Filho,O.A.; Queiroz,D.M.M.
Although Helicobacter heilmannii infection is less common than H. pylori infection in humans, it is considered to be of medical importance because of its association with gastritis, gastric ulcer, carcinoma, and mucosa-associated lymphoid tissue lymphoma of the stomach. However, there have been no studies evaluating the role of the Th cell response in H. heilmannii gastric infection. We evaluated the participation of pro-inflammatory and anti-inflammatory cytokines, IFN-gamma and IL-4, in H. heilmannii gastric infection in genetically IFN-gamma- or IL-4-deficient mice. The serum IFN-gamma and IL-4 concentrations were determined by ELISA. The gastric polymorphonuclear infiltrate was higher (P = 0.007) in H. heilmannii-positive than in H. heilmannii-negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter heilmannii; Gastric mucosa inflammation; IFN-gamma-/- mice; IL-4-/- mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200012

Endogenous opiate analgesia induced by tonic immobility in guinea pigs
Provedor de dados: 56
Autores: Leite-Panissi,C.R.A.; Rodrigues,C.L.; Brentegani,M.R.; Menescal-de-Oliveira,L.
A function of the endogenous analgesic system is to prevent recuperative behaviors generated by tissue damage, thus preventing the emission of species-specific defensive behaviors. Activation of intrinsic nociception is fundamental for the maintenance of the behavioral strategy adopted. Tonic immobility (TI) is an inborn defensive behavior characterized by a temporary state of profound and reversible motor inhibition elicited by some forms of physical restraint. We studied the effect of TI behavior on nociception produced by the formalin and hot-plate tests in guinea pigs. The induction of TI produced a significant decrease in the number of flinches (18 ± 6 and 2 ± 1 in phases 1 and 2) and lickings (6 ± 2 and 1 ± 1 in phases 1 and 2) in the formalin test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tonic immobility; Guinea pig; Antinociception; Opioids; Naloxone; Analgesia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200013

Improved biological performance of magnesium by micro-arc oxidation
Provedor de dados: 56
Autores: Ma,W.H.; Liu,Y.J.; Wang,W.; Zhang,Y.Z.
Magnesium and its alloys have recently been used in the development of lightweight, biodegradable implant materials. However, the corrosion properties of magnesium limit its clinical application. The purpose of this study was to comprehensively evaluate the degradation behavior and biomechanical properties of magnesium materials treated with micro-arc oxidation (MAO), which is a new promising surface treatment for developing corrosion resistance in magnesium, and to provide a theoretical basis for its further optimization and clinical application. The degradation behavior of MAO-treated magnesium was studied systematically by immersion and electrochemical tests, and its biomechanical performance when exposed to simulated body fluids was evaluated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnesium; Micro-arc oxidation; Biodegradable; Biocompatibility; Mechanical properties; Corrosion.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300214

Chemokines in the cerebrospinal fluid of patients with active and stable relapsing-remitting multiple sclerosis
Provedor de dados: 56
Autores: Moreira,M.A.; Souza,A.L.S.; Lana-Peixoto,M.A.; Teixeira,M.M.; Teixeira,A.L.
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the human central nervous system. Although its etiology is unknown, the accumulation and activation of mononuclear cells in the central nervous system are crucial to its pathogenesis. Chemokines have been proposed to play a major role in the recruitment and activation of leukocytes in inflammatory sites. They are divided into subfamilies on the basis of the location of conserved cysteine residues. We determined the levels of some CC and CXC chemokines in the cerebrospinal fluid (CSF) of 23 relapsing-remitting MS patients under interferon-ß-1a therapy and 16 control subjects using ELISA. MS patients were categorized as having active or stable disease. CXCL10 was significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CXCL10; CCL2; Inflammation; Multiple sclerosis; Leukocyte recruitment.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400003

Apolipoprotein E polymorphism in Brazilian dyslipidemic individuals: Ouro Preto study
Provedor de dados: 56
Autores: Mendes-Lana,A.; Pena,G.G.; Freitas,S.N.; Lima,A.A.; Nicolato,R.L.C.; Nascimento-Neto,R.M.; Machado-Coelho,G.L.L.; Freitas,R.N.
The influence of apolipoprotein E alleles and genotypes on plasma lipid levels was determined in 185 individuals of mixed ethnicity living in Ouro Preto, Brazil. DNA was obtained from blood samples and the genotypes were determined by an RFLP-PCR procedure. The *3 allele was the most frequent (72%), followed by *4 (20%) and *2 (8%); *4 frequency was higher and *2 frequency was lower in the dyslipidemic group than in the normal control group. The *2 carriers presented lower LDL and total cholesterol levels compared to the *3 and *4 carriers. All six expected genotypes were observed in the individuals genotyped: E2/2 (2.1%), E4/4 (2.7%), E2/4 (3.7%), E2/3 (8.0%), E3/3 (53.3%), E3/4 (29.9%); no difference in genotype frequencies was found between the normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E polymorphism; Lipids; Dyslipidemia; Brazilian population; Atherosclerosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100007

Use of inflammatory molecules to predict the occurrence of fever in onco-hematological patients with neutropenia
Provedor de dados: 56
Autores: Ribeiro,A.F. Tibúrcio; Nobre,V.; Neuenschwander,L.C.; Teixeira,A.L.; Xavier,S.G.; Paula,F.D.F.; Teixeira,M.M.; Teixeira,J.C.A.; Bittencourt,H.
Febrile neutropenia remains a frequent complication in onco-hematological patients, and changes in the circulating level of inflammatory molecules (IM) may precede the occurrence of fever. The present observational prospective study was carried out to evaluate the behavior of plasma tumor necrosis factor alpha (TNF-α), soluble TNF-α I and II receptors (sTNFRI and sTNFRII), monocyte chemoattractant protein-1 [MCP-1 or chemokine (c-c motif) ligand 2 (CCL2)], macrophage inflammatory protein-1α (MIP-1α or CCL3), eotaxin (CCL11), interleukin-8 (IL-8 or CXCL8), and interferon-inducible protein-10 (IP-10 or CXCL10) in 32 episodes of neutropenia in 26 onco-hematological patients. IM were tested on enrollment and 24-48 h before the onset of fever and within 24 h of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutropenia; Cytokines; Oncological hematology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200200

Resistance training attenuates salt overload-induced cardiac remodeling and diastolic dysfunction in normotensive rats
Provedor de dados: 56
Autores: Barretti,D.L.M.; Melo,S.F.S.; Oliveira,E.M.; Barauna,V.G.
Elevated salt intake induces changes in the extracellular matrix collagen, leading to myocardial stiffness and impaired relaxation. Resistance training (RT) has been used as a remarkably successful strategy in the treatment of heart disease. Therefore, the aim of this study was to investigate the effects of RT on preventing pathological adaptation of the left ventricle (LV) induced by salt overload. Male Wistar rats (10 weeks old) were distributed into four groups (n=8/group): control (CO), control+1% salt (CO+SALT), RT and RT+1% salt (RT+SALT). The RT protocol consisted of 4×12 bouts of squat training, 5/week for 8 weeks, with 80% of one repetition maximum (1RM). Echocardiographs were analyzed and interstitial collagen volume fraction (CVF) was determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diastolic dysfunction; Interstitial collagen; Resistance training; Salt overload.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900603

The Streptococcus mutans GlnR protein exhibits an increased affinity for the glnRA operon promoter when bound to GlnK
Provedor de dados: 56
Autores: Castellen,P.; Rego,F.G.M.; Portugal,M.E.G.; Benelli,E.M.
The control of nitrogen metabolism in pathogenic Gram-positive bacteria has been studied in a variety of species and is involved with the expression of virulence factors. To date, no data have been reported regarding nitrogen metabolism in the odontopathogenic species Streptococcus mutans. GlnR, which controls nitrogen assimilation in the related bacterial species, Bacillus subtilis, was assessed in S. mutans for its DNA and protein binding activity. Electrophoretic mobility shift assay of the S. mutans GlnR protein indicated that GlnR binds to promoter regions of the glnRA and amtB-glnK operons. Cross-linking and pull-down assays demonstrated that GlnR interacts with GlnK, a signal transduction protein that coordinates the regulation of nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus mutans; GlnR; Transcriptional regulator; GlnK; PII-type protein.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200002

A high-fructose diet induces changes in pp185 phosphorylation in muscle and liver of rats
Provedor de dados: 56
Autores: Ueno,M.; Bezerra,R.M.N.; Silva,M.S.; Tavares,D.Q.; Carvalho,C.R.; Saad,M.J.A.
Insulin stimulates the tyrosine kinase activity of its receptor resulting in the tyrosine phosphorylation of pp185, which contains insulin receptor substrates IRS-1 and IRS-2. These early steps in insulin action are essential for the metabolic effects of insulin. Feeding animals a high-fructose diet results in insulin resistance. However, the exact molecular mechanism underlying this effect is unknown. In the present study, we determined the levels and phosphorylation status of the insulin receptor and pp185 (IRS-1/2) in liver and muscle of rats submitted to a high-fructose diet evaluated by immunoblotting with specific antibodies. Feeding fructose (28 days) induced a discrete insulin resistance, as demonstrated by the insulin tolerance test. Plasma...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fructose; Insulin receptor; Pp185 phosphorylation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200004

Precipitated immune complexes of IgM induce the generation of reactive oxygen species by rabbit polymorphonuclear leucocytes
Provedor de dados: 56
Autores: Lucisano,Y.M.; de-Mello,A.M.; Vasques,E.R.; Mantovani,B.
We report that immune complexes of IgM (ICIgM) antibodies and ovalbumin in the form of a precipitate from the equivalence zone induce the generation of reactive oxygen species by rabbit blood polymorphonuclear leucocytes (PMN), as measured by the chemiluminescence (CL) production in the presence of luminol. The kinetics of CL generation induced by ICIgM is quite different from that induced by precipitated immune complexes of IgG (ICIgG): the maximum rate of CL production for ICIgM occurs around 14 min, whereas for ICIgG it occurs about 5 min after incubation with the cells. Also the triggering of the process requires a higher concentration of ICIgM than of ICIgG. Evidence is presented that these effects are not mediated by interaction of the antigen...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Reactive oxygen species; Polymorphonuclear leucocytes; Immune complexes; IgM; IgG.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600011

Gene expression profiling reveals upregulated FUT1 and MYBPC1 in children with pancreaticobiliary maljunction
Provedor de dados: 56
Autores: Guo,Wan-Liang; Geng,Jia; Zhao,Jun-gang; Fang,Fang; Huang,Shun-Gen; Wang,Jian.
Pancreaticobiliary maljunction (PBM) is associated with high risk of epithelial atypical growth and malignant transformation of the bile duct or gallbladder. However, overall changes in genetic expression have not been examined in children with PBM. Genome-wide expression was analyzed using peripheral blood samples from 10 children with PBM and 15 pediatric controls. Differentially expressed genes (DEGs) were identified using microarray. Bioinformatics analysis was conducted using Gene Ontology and KEGG analyses. The top 5 in the up-regulated genes in PBM were verified with qRT-PCR. Receiver operator characteristic curve analysis was conducted to evaluate the predictive accuracy of selected genes for PBM. The microarray experiments identified a total of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pediatric; Pancreaticobiliary maljunction; Gene expression.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800603

Albendazole metabolism in patients with neurocysticercosis: antipyrine as a multifunctional marker drug of cytochrome P450
Provedor de dados: 56
Autores: Marques,M.P.; Takayanagui,O.M.; Lanchote,V.L.
The present study investigates the isoform(s) of cytochrome P450 (CYP) involved in the metabolism of albendazole sulfoxide (ASOX) to albendazole sulfone (ASON) in patients with neurocysticercosis using antipyrine as a multifunctional marker drug. The study was conducted on 11 patients with neurocysticercosis treated with a multiple dose regimen of albendazole for 8 days (5 mg/kg every 8 h). On the 5th day of albendazole treatment, 500 mg antipyrine was administered po. Blood and urine samples were collected up to 72 h after antipyrine administration. Plasma concentrations of (+)-ASOX, (-)-ASOX and ASON were determined by HPLC using a chiral phase column and detection by fluorescence. The apparent clearance (CL/f) of ASON and of the (+) and (-)-ASOX...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albendazole; Cysticercosis; Enantiomers; Antipyrine; Cytochrome P450; CYP; Patients.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200016

A conjugate of an anti-midkine single-chain variable fragment to doxorubicin inhibits tumor growth
Provedor de dados: 56
Autores: Zhao,Shuli; Zhao,Guangfeng; Xie,Hao; Huang,Yahong; Hou,Yayi.
Doxorubicin (DOX) was conjugated to a single-chain variable fragment (scFv) against human midkine (MK), and the conjugate (scFv-DOX) was used to target the chemotherapeutic agent to a mouse solid tumor model in which the tumor cells expressed high levels of human MK. The His-tagged recombinant scFv was expressed in bacteria, purified by metal affinity chromatography, and then conjugated to DOX using oxidative dextran (Dex) as a linker. The molecular formula of this immunoconjugate was scFv(Dex)1.3(DOX)20. In vitro apoptosis assays showed that the scFv-DOX conjugate was more cytotoxic against MK-transfected human adenocarcinoma cells (BGC823-MK) than untransfected cells (55.3 ± 2.4 vs 22.4 ± 3.8%) for three independent experiments. Nude mice bearing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single-chain variable fragments; Midkine; Tumor targeting; Doxorubicin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300008

Efficacy, acceptability, and tolerability of antidepressant treatments for patients with post-stroke depression: a network meta-analysis
Provedor de dados: 56
Autores: Qin,B.; Chen,H.; Gao,W.; Zhao,L.B.; Zhao,M.J.; Qin,H.X.; Chen,W.; Chen,L.; Yang,M.X.
The aim of this study was to investigate the efficacy, acceptability, and tolerability of antidepressants in treating post-stroke depression (PSD) by performing a network meta-analysis of randomized controlled trials of the current literature. Eligible studies were retrieved from online databases, and relevant data were extracted. The primary outcome was efficacy as measured by the mean change in overall depressive symptoms. Secondary outcomes included discontinued treatment for any reason and specifically due to adverse events. Fourteen trials were eligible, which included 949 participants and 9 antidepressant treatments. Few significant differences were found for all outcomes. For the primary outcome, doxepin, paroxetine, and nortriptyline were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antidepressants; Post-stroke depression; Randomized controlled trials; Network meta-analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700601

Relationship between heart rate variability and subclinical thyroid disorders of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Provedor de dados: 56
Autores: de Miranda,É.J.F. Peixoto; Hoshi,R.A.; Bittencourt,M.S.; Goulart,A.C.; Santos,I.S.; Brunoni,A.R.; Diniz,M.F.H.S.; Ribeiro,A.L.P.; Dantas,E.M.; Mill,J.G.; Lotufo,P.A.; Benseñor,I.M.
The association between subclinical thyroid dysfunctions and autonomic modulation changes has been described by many studies with conflicting results. We aimed to analyze the association between subclinical hyperthyroidism (SCHyper), subclinical hypothyroidism (SCHypo), and heart rate variability (HRV) using the baseline from ELSA-Brasil. SCHyper and SCHypo were classified by use of medication to treat thyroid disorders, thyrotropin levels respectively above and under the reference range, and normal free thyroxine levels. For HRV, the participants underwent 10 min in supine position and the R-R intervals of the final 5 min were selected for analysis. We first used linear regression models to report crude data and then, multivariate adjustment for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subclinical hypothyroidism; Subclinical hyperthyroidism; Heart rate variability; Autonomic nervous system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100601

Multiple facets of HIV-associated renal disease
Provedor de dados: 56
Autores: da Silva,D.R.; Gluz,I.C.; Kurz,J.; Thomé,G.G.; Zancan,R.; Bringhenti,R.N.; Schaefer,P.G.; dos Santos,M.; Barros,E.J.G.; Veronese,F.V.
HIV infection has a broad spectrum of renal manifestations. This study examined the clinical and histological manifestations of HIV-associated renal disease, and predictors of renal outcomes. Sixty-one (64% male, mean age 45 years) HIV patients were retrospectively evaluated. Clinical presentation and renal histopathology were assessed, as well as CD4 T-cell count and viral load. The predictive value of histological lesion, baseline CD4 cell count and viral load for end-stage renal disease (ESRD) or death were determined using the Cox regression model. The outcomes of chronic kidney disease (CKD) and ESRD or death were evaluated by baseline CD4 cell count. The percent distribution at initial clinical presentation was non-nephrotic proteinuria (54%), acute...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Renal disease; Proteinuria; Collapsing focal segmental glomerulosclerosis; CD4 cell count; Chronic kidney disease.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400709

Bone marrow fibroblasts in patients with advanced lung cancer
Provedor de dados: 56
Autores: Chasseing,N.A.; Hofer,E.; Bordenave,R.H.; Shanley,C.; Rumi,L.S.
In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblasts; Interleukin-1; Prostaglandin E2; Cancer.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100014

Treatment delay and radiological errors in patients with bone metastases
Provedor de dados: 56
Autores: Ichinohe,K.; Takahashi,M.; Tooyama,N.
During routine investigations, we are surprised to find that therapy for bone metastases is sometimes delayed for a considerable period of time. To determine the extent of this delay and its causes, we reviewed the medical records of symptomatic patients seen at our hospital who had been recently diagnosed as having bone metastases for the last four years. The treatment delay was defined as the interval between presentation with symptoms and definitive treatment for bone metastases. The diagnostic delay was defined as the interval between presentation with symptoms and diagnosis of bone metastases. The results of diagnostic radiological examinations were also reviewed for errors. The study population included 76 males and 34 females with a median age of 66...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone metastases; Delay; Radiology; Error; Radiotherapy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000020

Occupational therapy in spinocerebellar ataxia type 3: an open-label trial
Provedor de dados: 56
Autores: Silva,R.C.R.; Saute,J.A.M.; Silva,A.C.F.; Coutinho,A.C.O.; Saraiva-Pereira,M.L.; Jardim,L.B.
Occupational therapy (OT) is a profession concerned with promoting health and well-being through occupation, by enabling handicapped people to participate in the activities of everyday life. OT is part of the clinical rehabilitation of progressive genetic neurodegenerative diseases such as spinocerebellar ataxias; however, its effects have never been determined in these diseases. Our aim was to investigate the effect of OT on both physical disabilities and depressive symptoms of spinocerebellar ataxia type 3 (SCA3) patients. Genomically diagnosed SCA3 patients older than 18 years were invited to participate in the study. Disability, as evaluated by functional independence measurement and Barthel incapacitation score, Hamilton Rating Scale for Depression,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinocerebellar ataxia 3; Occupational therapy; Rehabilitation; Depression; Machado-Joseph disease; Polyglutamine diseases.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600003

Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition
Provedor de dados: 56
Autores: Zhou,Yuan; Xue,Ruifeng; Wang,Jinglin; Ren,Haozhen.
Hepatocellular carcinoma (HCC) is one of the most common primary malignant tumors of the liver worldwide. Liver resection and transplantation are currently the only effective treatments; however, recurrence and metastasis rates are still high. Previous studies have shown that the epithelial-mesenchymal transition (EMT) is a key step in HCC invasion and metastasis. Inhibition of EMT has become a new therapeutic strategy for tumors. Recently, puerarin, a well-characterized component of traditional Chinese medicine, has been isolated from Pueraria radix and exerts positive effects on many diseases, particularly cancers. In this study, CCK-8, EdU immunofluorescence, colony formation, wound healing, and migration assays were used to detect the effects of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Metastasis; Epithelial-mesenchymal transition; PTEN; MiR-21.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400611

Effects of a 4-week high-intensity interval training on pacing during 5-km running trial
Provedor de dados: 56
Autores: Silva,R.; Damasceno,M.; Cruz,R.; Silva-Cavalcante,M.D.; Lima-Silva,A.E.; Bishop,D.J.; Bertuzzi,R.
This study analyzed the influence of a 4-week high-intensity interval training on the pacing strategy adopted by runners during a 5-km running trial. Sixteen male recreational long-distance runners were randomly assigned to a control group (CON, n=8) or a high-intensity interval training group (HIIT, n=8). The HIIT group performed high-intensity interval-training twice per week, while the CON group maintained their regular training program. Before and after the training period, the runners performed an incremental exercise test to exhaustion to measure the onset of blood lactate accumulation, maximal oxygen uptake (VO2max), and peak treadmill speed (PTS). A submaximal constant-speed test to measure the running economy (RE) and a 5-km running trial on an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rating of perceived exertion; Running economy; Peak treadmill speed; Maximal oxygen uptake.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200612

Receptor localization of steroid hormones and drugs: discoveries through the use of thaw-mount and dry-mount autoradiography
Provedor de dados: 56
Autores: Stumpf,W.E.
The history of receptor autoradiography, its development and applications, testify to the utility of this histochemical technique for localizing radiolabeled hormones and drugs at cellular and subcellular sites of action in intact tissues. Localization of diffusible compounds has been a challenge that was met through the introduction of the "thaw-mount" and "dry-mount" autoradiographic techniques thirty years ago. With this cellular receptor autoradiography, used alone or combined with other histochemical techniques, sites of specific binding and deposition in vivo and in vitro have been characterized. Numerous discoveries, some reviewed in this article, provided information that led to new concepts and opened new areas of research. As an example, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin D; Steroid hormones; Receptors; Autoradiography; Drug distribution; Localization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200003

Cyst infection in hospital-admitted autosomal dominant polycystic kidney disease patients is predominantly multifocal and associated with kidney and liver volume
Provedor de dados: 56
Autores: Balbo,B.E.P.; Sapienza,M.T.; Ono,C.R.; Jayanthi,S.K.; Dettoni,J.B.; Castro,I.; Onuchic,L.F.
Positron-emission tomography/computed tomography (PET/CT) has improved cyst infection (CI) management in autosomal dominant polycystic kidney disease (ADPKD). The determinants of kidney and/or liver involvement, however, remain uncertain. In this study, we evaluated clinical and imaging factors associated with CI in kidney (KCI) and liver (LCI) in ADPKD. A retrospective cohort study was performed in hospital-admitted ADPKD patients with suspected CI. Clinical, imaging and surgical data were analyzed. Features of infected cysts were evaluated by PET/CT. Total kidney (TKV) and liver (TLV) volumes were measured by CT-derived multiplanar reconstruction. CI was detected in 18 patients who experienced 24 episodes during an interval of 30 months (LCI in 12, KCI...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autosomal dominant polycystic kidney disease; Cyst infection; Positron-emission tomography/computed tomography; Kidney volume; Liver volume; Mortality.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700584

Lead reduces tension development and the myosin ATPase activity of the rat right ventricular myocardium
Provedor de dados: 56
Autores: Vassallo,D.V.; Lebarch,E.C.; Moreira,C.M.; Wiggers,G.A.; Stefanon,I.
Lead (Pb2+) poisoning causes hypertension, but little is known regarding its acute effects on cardiac contractility. To evaluate these effects, force was measured in right ventricular strips that were contracting isometrically in 45 male Wistar rats (250-300 g) before and after the addition of increasing concentrations of lead acetate (3, 7, 10, 30, 70, 100, and 300 µM) to the bath. Changes in rate of stimulation (0.1-1.5 Hz), relative potentiation after pauses of 15, 30, and 60 s, effect of Ca2+ concentration (0.62, 1.25, and 2.5 mM), and the effect of isoproterenol (20 ng/mL) were determined before and after the addition of 100 µM Pb2+. Effects on contractile proteins were evaluated after caffeine treatment using tetanic stimulation (10 Hz) and measuring...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lead; Right ventricular strips; Contraction; Heart; Myosin ATPase activity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900008

Influence of cueing on the preparation and execution of untrained and trained complex motor responses
Provedor de dados: 56
Autores: Alouche,S.R.; Sant’Anna,G.N.; Biagioni,G.; Ribeiro-do-Valle,L.E.
This study investigated the influence of cueing on the performance of untrained and trained complex motor responses. Healthy adults responded to a visual target by performing four sequential movements (complex response) or a single movement (simple response) of their middle finger. A visual cue preceded the target by an interval of 300, 1000, or 2000 ms. In Experiment 1, the complex and simple responses were not previously trained. During the testing session, the complex response pattern varied on a trial-by-trial basis following the indication provided by the visual cue. In Experiment 2, the complex response and the simple response were extensively trained beforehand. During the testing session, the trained complex response pattern was performed in all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cueing; Cue-target interval; Response complexity; Response practice; Reaction time; Movement time.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500008

Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12
Provedor de dados: 56
Autores: Camargo,A.A.; Nunes,D.N.; Samaia,H.B.; Liu,L.; Collins,V.P.; Simpson,A.J.G.; Dias-Neto,E.
DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DDX26; DEAD-box RNA helicase; 7p12; Ribosome biogenesis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000002

Angiotensin-(1-7) relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis
Provedor de dados: 56
Autores: Lu,W.; Kang,J.; Hu,K.; Tang,S.; Zhou,X.; Yu,S.; Xu,L.
We aimed to study the renal injury and hypertension induced by chronic intermittent hypoxia (CIH) and the protective effects mediated by angiotensin 1-7 [Ang(1-7)]. We randomly assigned 32 male Sprague-Dawley rats (body weight 180-200 g) to normoxia control, CIH, Ang(1-7)-treated normoxia, and Ang(1-7)-treated CIH groups. Systolic blood pressure (SBP) was monitored at the start and end of each week. Renal sympathetic nerve activity (RSNA) was recorded. CTGF and TGF-β were detected by immunohistochemistry and western blotting. Tissue parameters of oxidative stress were also determined. In addition, renal levels of interleukin-6, tumor necrosis factor-α, nitrotyrosine, and hypoxia-inducible factor-1α were determined by immunohistochemistry, immunoblotting,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ang(1-7); Renal injury; Obstructive sleep apnea; Inflammation; Oxidative stress; Fibrosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100604

Prostate-specific membrane antigen can promote in vivo osseous metastasis of prostate cancer cells in mice
Provedor de dados: 56
Autores: Zhao,Liang-Yun; Mao,Xiao-Peng; Chao,Kai-Yuan; Guo,Sheng-Jie; Qiu,Shao-Peng.
Reports remain insufficient on whether and how prostate-specific membrane antigen (PSMA) can influence in vivo osseous metastasis of prostate cancer (PCa). In the present study, the authors induced stable expression of PSMA in mouse PCa cell line RM-1. In vivo osseous metastasis was induced in 37 6-week-old female C57BL/6 mice weighing 22.45 ± 0.456 g. RM-1 cells were actively injected into the femoral bone cavity, leading to bilateral dissymmetry of bone density in the femoral bone. Tumor cells were also detected in bone tissue by pathological examination. The impact on bone density was demonstrated by the significant difference between animals injected with RM-PSMA cells (0.0738 ± 0.0185 g/cm²) and animals injected with RM-empty plasmid cells (0.0895 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate carcinoma; Prostate-specific membrane antigen; In vitro; Osseous metastasis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800008

Fish hemoglobins
Provedor de dados: 56
Autores: Souza,P.C. de; Bonilla-Rodriguez,G.O.
Vertebrate hemoglobin, contained in erythrocytes, is a globular protein with a quaternary structure composed of 4 globin chains (2 alpha and 2 beta) and a prosthetic group named heme bound to each one. Having myoglobin as an ancestor, hemoglobin acquired the capacity to respond to chemical stimuli that modulate its function according to tissue requirements for oxygen. Fish are generally submitted to spatial and temporal O2 variations and have developed anatomical, physiological and biochemical strategies to adapt to the changing environmental gas availability. Structurally, most fish hemoglobins are tetrameric; however, those from some species such as lamprey and hagfish dissociate, being monomeric when oxygenated and oligomeric when deoxygenated. Fish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemoglobins; Fish; Root effect; Bohr effect.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600004

Serotonin regulates osteoblast proliferation and function in vitro
Provedor de dados: 56
Autores: Dai,S.Q.; Yu,L.P.; Shi,X.; Wu,H.; Shao,P.; Yin,G.Y.; Wei,Y.Z.
The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; Osteoblast; Proliferation; Differentiation; 5-HT receptors; 5-HT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900759

Excavation of attractor modules for nasopharyngeal carcinoma via integrating systemic module inference with attract method
Provedor de dados: 56
Autores: Jiang,T.; Jiang,C.-Y.; Shu,J.-H.; Xu,Y.-J.
The molecular mechanism of nasopharyngeal carcinoma (NPC) is poorly understood and effective therapeutic approaches are needed. This research aimed to excavate the attractor modules involved in the progression of NPC and provide further understanding of the underlying mechanism of NPC. Based on the gene expression data of NPC, two specific protein-protein interaction networks for NPC and control conditions were re-weighted using Pearson correlation coefficient. Then, a systematic tracking of candidate modules was conducted on the re-weighted networks via cliques algorithm, and a total of 19 and 38 modules were separately identified from NPC and control networks, respectively. Among them, 8 pairs of modules with similar gene composition were selected, and 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nasopharyngeal carcinoma; Protein-protein interaction; Attract; Module; Cell division.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800602

Do whole-body vibration exercise and resistance exercise modify concentrations of salivary cortisol and immunoglobulin A?
Provedor de dados: 56
Autores: Roschel,H.; Barroso,R.; Batista,M.; Ugrinowitsch,C.; Tricoli,V.; Arsati,F.; Lima-Arsati,Y.B.; Araújo,V.C.; Moreira,A.
A single bout of resistance exercise (RE) induces hormonal and immune responses, playing an important role in a long-term adaptive process. Whole-body vibration (WBV) has also been shown to affect hormonal responses. Evidence suggests that combining WBV with RE may amplify hormonal and immune responses due to the increased neuromuscular load. Therefore, the aim of this study was to evaluate salivary cortisol (Scortisol) and salivary IgA (SIgA) concentrations following a RE session combined or not with WBV. Nine university students (22.9 ± 5.1 years, 175.8 ± 5.2 cm, and 69.2 ± 7.3 kg) performed five sets of squat exercise (70% one-repetition-maximum) combined (R+V30) or not (R) with WBV at 30 Hz. Saliva samples were obtained before and after exercise....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Upper respiratory tract infection; Hormonal responses; Immune responses; Neuromuscular training.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600013

Serum hexosaminidase and ß-glucuronidase activities in infants: effects of age and sex
Provedor de dados: 56
Autores: Mabe,P.; Beck,M.
We investigated the effect of age and sex on the serum activity of hexosaminidase (HEX) and ß-glucuronidase (BGLU) in 275 normal term infants aged 12 h to 12 months. Up to six weeks of life, HEX was significantly higher in boys (P&lt;=0.023). During the age period of 1-26 weeks, BGLU was also higher in boys, but differences were significant only at 2-6 and 7-15 weeks (P&lt;=0.016). The developmental pattern of HEX and BGLU was sex dependent. HEX activity increased in both sexes from 4-7 days of life, reaching a maximum of 1.4-fold the birth value at 2-6 weeks of age in boys (P&lt;0.001) and a maximum of 1.6-fold at 7-15 weeks in girls (P&lt;0.001). HEX activity gradually decreased thereafter, reaching significantly lower levels at 27-53 weeks than during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysosomal storage disease; GM2 gangliosidosis; Mucopolysaccharidosis type VII; Hexosaminidase; SS-Glucuronidase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300013

Impact of initial exposure to calcineurin inhibitors on kidney graft function of patients at high risk to develop delayed graft function
Provedor de dados: 56
Autores: Silva,L.A.; Felipe,C.R.; Park,S.I.; Pinheiro-Machado,P.; Garcia,R.; Franco,M.; Moreira,S.R.; Tedesco-Silva Jr.,H.; Medina-Pestana,J.
We conducted a retrospective analysis of the influence of full doses of calcineurin inhibitors [8-10 mg kg-1 day-1 cyclosporine (N = 80), or 0.2-0.3 mg kg-1 day-1 tacrolimus (N = 68)] administered from day 1 after transplantation on the transplant outcomes of a high-risk population. Induction therapy was used in 13% of the patients. Patients also received azathioprine (2 mg kg-1 day-1, N = 58) or mycophenolate mofetil (2 g/day, N = 90), and prednisone (0.5 mg kg-1 day-1, N = 148). Mean time on dialysis was 79 ± 41 months, 12% of the cases were re-transplants, and 21% had panel reactive antibodies &gt;10%. In 43% of donors the cause of death was cerebrovascular disease and 27% showed creatinine above 1.5 mg/dL. The incidence of slow graft function (SGF) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclosporine; Tacrolimus; Delayed graft function; Graft function; Kidney transplantation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100005

Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release
Provedor de dados: 56
Autores: Chini,E.N.
Cells possess multiple intracellular Ca2+-releasing systems. Sea urchin egg homogenates are a well-established model to study intracellular Ca2+ release. In the present study the mechanism of interaction between three intracellular Ca2+ pools, namely the nicotinic acid adenine dinucleotide phosphate (NAADP), the cyclic ADP-ribose (cADPR) and the inositol 1',4',5'-trisphosphate (IP3)-regulated Ca2+ stores, is explored. The data indicate that the NAADP Ca2+ pool could be used to sensitize the cADPR system. In contrast, the IP3 pool was not affected by the Ca2+ released by NAADP. The mechanism of potentiation of the cADPR-induced Ca2+ release, promoted by Ca2+ released from the NAADP pool, is mediated by the mechanism of Ca2+-induced Ca2+ release. These data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CADPR; NAADP; Calcium; Sea urchin eggs; Fertilization; IP3.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500005

Correlation of ultrasonography synovitis with disease activity and clinical response to etanercept treatment in juvenile idiopathic arthritis patients
Provedor de dados: 56
Autores: Zhou,Li; Gu,Xiaojie.
This study aimed to investigate the correlation of ultrasonography (US) of synovitis with disease activity and clinical response to etanercept (ETN) in juvenile idiopathic arthritis (JIA) patients. Eighty-two JIA patients who underwent ETN treatment for 24 weeks were consecutively enrolled. US evaluations of 28 joints (shoulder, elbow, wrist, metacarpophalangeal, and proximal interphalangeal of hands and knee) at baseline were performed using grey-scale US and power doppler (PD) US, and US synovitis was defined as grey-scale abnormalities or PD abnormalities. Clinical response was assessed according to the ACRpedi 50 response criteria. In total, 2296 joints were scanned and 608 (26.5%) joints presented US synovitis, which was numerically higher than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrasonography Synovitis; Disease activity Clinical response Etanercept.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200603

STIM1/Orai1-mediated store-operated Ca2+ entry: the tip of the iceberg
Provedor de dados: 56
Autores: Giachini,F.R.; Lima,V.V.; Hannan,J.L.; Carneiro,F.S.; Webb,R.C.; Tostes,R.C.
Highly efficient mechanisms regulate intracellular calcium (Ca2+) levels. The recent discovery of new components linking intracellular Ca2+ stores to plasma membrane Ca2+ entry channels has brought new insight into the understanding of Ca2+ homeostasis. Stromal interaction molecule 1 (STIM1) was identified as a Ca2+ sensor essential for Ca2+ store depletion-triggered Ca2+ influx. Orai1 was recognized as being an essential component for the Ca2+ release-activated Ca2+ (CRAC) channel. Together, these proteins participate in store-operated Ca2+ channel function. Defective regulation of intracellular Ca2+ is a hallmark of several diseases. In this review, we focus on Ca2+ regulation by the STIM1/Orai1 pathway and review evidence that implicates STIM1/Orai1 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+ release-activated Ca2+ channel; Sarcoplasmic reticulum; Intracellular Ca2+ store; Ca2+ influx.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100002

Follow-up of patients treated with retinoic acid for the control of radioiodine non-responsive advanced thyroid carcinoma
Provedor de dados: 56
Autores: Coelho,S.M.; Vaisman,F.; Buescu,A.; Mello,R.C.R.; Carvalho,D.P.; Vaisman,M.
During thyroid tumor progression, cellular de-differentiation may occur and it is commonly accompanied by metastatic spread and loss of iodine uptake. Retinoic acid (RA) administration might increase iodine uptake in about 40% of patients, suggesting that RA could be a promising therapeutic option for radioiodine non-responsive thyroid carcinoma, although a prospective study with a long-term follow-up has not been reported. This was a clinical prospective study assessing the value of 13-cis-RA in patients with advanced thyroid carcinoma and its impact on major outcomes such as tumor regression and cancer-related death with a long-term follow-up of patients submitted to radioiodine (131I) therapy after RA administration. Sixteen patients with inoperable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid cancer; Retinoic acid; Radioiodine therapy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100011

Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro
Provedor de dados: 56
Autores: Yang,Xia; Qian,Feng; He,Hai-Yang; Liu,Kai-Jun; Lan,Yuan-Zhi; Ni,Bing; Tian,Yi; Fu,Xiao-Lan; Zhang,Ji; Shen,Zi-Gang; Li,Jian; Yin,Yi; Li,Jin-Tao; Wu,Yu-Zhang.
Thymosin alpha 1 (Tα1) has been shown to have beneficial effects on numerous immune system parameters, but little is known about the effects of Tα1 on patients with gastric carcinoma. The objective of this study was to determine the effect of Tα1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro, and to evaluate its efficacy as an immunoregulatory factor in patients with gastric carcinoma. We compared the effect of Tα1 on the frequency of CD4+ and CD8+ T cells, especially the CD4+CD25+Foxp3+ Tregs in peripheral blood mononuclear cells (PBMCs) from gastric carcinoma patients (N = 35) and healthy donors (N = 22). We also analyzed the changes in the proliferation of PBMCs in response to treatment with Tα1, and examined the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tα1; Gastric carcinoma; Tregs; Th1; Th2; Th17.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100005

Electrophysiological evidence for the presence of NR2C subunits of N-methyl-D-aspartate receptors in rat neurons of the nucleus tractus solitarius
Provedor de dados: 56
Autores: Baptista,V.; Ogawa,W.N.; Aguiar,J.F.; Varanda,W.A.
The nucleus tractus solitarius (NTS) plays an important role in the control of autonomic reflex functions. Glutamate, acting on N-methyl-D-aspartate (NMDA) and non-NMDA ionotropic receptors, is the major neurotransmitter in this nucleus, and the relative contribution of each receptor to signal transmission is unclear. We have examined NMDA excitatory postsynaptic currents (NMDA-EPSCs) in the subpostremal NTS using the whole cell patch clamp technique on a transverse brainstem slice preparation. The NMDA-EPSCs were evoked by stimulation of the solitary tract over a range of membrane potentials. The NMDA-EPSCs, isolated pharmacologically, presented the characteristic outward rectification and were completely blocked by 50 µM DL-2-amino-5-phosphonopentanoic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nucleus tractus solitarius; Electrophysiology; Synapse; NMDA receptors; NR2C subunits.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100016

Indole ring oxidation by activated leukocytes prevents the production of hypochlorous acid
Provedor de dados: 56
Autores: Ximenes,V.F.; Paino,I.M.M.; Faria-Oliveira,O.M.M. de; Fonseca,L.M. da; Brunetti,I.L.
Hypochlorous acid (HOCl) released by activated leukocytes has been implicated in the tissue damage that characterizes chronic inflammatory diseases. In this investigation, 14 indole derivatives, including metabolites such as melatonin, tryptophan and indole-3-acetic acid, were screened for their ability to inhibit the generation of this endogenous oxidant by stimulated leukocytes. The release of HOCl was measured by the production of taurine-chloramine when the leukocytes (2 x 10(6) cells/mL) were incubated at 37ºC in 10 mM phosphate-buffered saline, pH 7.4, for 30 min with 5 mM taurine and stimulated with 100 nM phorbol-12-myristate acetate. Irrespective of the group substituted in the indole ring, all the compounds tested including indole,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukocytes; Myeloperoxidase; Hypochlorous acid; Indole ring; Melatonin; Indole-3-acetic acid.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100003

Adrenergic receptor β3 is involved in the memory consolidation process in mice
Provedor de dados: 56
Autores: Souza-Braga,P.; Lorena,F.B.; Nascimento,B.P.P.; Marcelino,C.P.; Ravache,T.T.; Ricci,E.; Bernardi,M.M.; Ribeiro,M.O.
Attention and emotion have a positive impact on memory formation, which is related to the activation of the noradrenergic system in the brain. The hippocampus and amygdala are fundamental structures in memory acquisition, which is modulated by noradrenaline through the noradrenergic receptors. Pharmacological studies suggest that memory acquisition depends on the action of both the β3 (β3-AR) and β2 (β2-AR) receptor subtypes. However, the use of animal models with specific knockout for the β3-AR receptor only (β3-ARKO) allows researchers to more accurately assess its role in memory formation processes. In the present study, we evaluated short- and long-term memory acquisition capacity in β3-ARKO mice and wild-type mice at approximately 60 days of age. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenergic receptors; Memory; GLUT3; Β3-AR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000607

E. coli a-hemolysin: a membrane-active protein toxin
Provedor de dados: 56
Autores: Goñi,F.M.; Ostolaza,H.
Alpha-Hemolysin is synthesized as a 1024-amino acid polypeptide, then intracellularly activated by specific fatty acylation. A second activation step takes place in the extracellular medium through binding of Ca2+ ions. Even in the absence of fatty acids and Ca2+ HlyA is an amphipathic protein, with a tendency to self-aggregation. However, Ca2+-binding appears to expose hydrophobic patches on the protein surface, facilitating both self-aggregation and irreversible insertion into membranes. The protein may somehow bind membranes in the absence of divalent cations, but only when Ca2+ (or Sr2+, or Ba2+) is bound to the toxin in aqueous suspensions, i.e., prior to its interaction with bilayers, can &lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-hemolysin bind...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-hemolysin; Bacterial toxins; RTX toxins; Lipid-protein interactions; Calcium-binding proteins; Model membranes.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800002

Cognitive performance of young and elderly subjects on the free word recall memory test: effect of presentation order on recall order
Provedor de dados: 56
Autores: Santos-Galduróz,R.F.; Oliveira,F.G.; Galduróz,J.C.F.; Bueno,O.F.A.
The influence of aging on memory has been extensively studied, but the importance of short-term memory and recall sequence has not. The objective of the current study was to examine the recall order of words presented on lists and to determine if age affects recall sequence. Physically and psychologically healthy male subjects were divided into two groups according to age, i.e., 23 young subjects (20 to 30 years) and 50 elderly subjects (60 to 70 years) submitted to the Wechsler Adult Intelligence Scale-Revised and the free word recall test. The order of word presentation significantly affected the 3rd and 4th words recalled (P &lt; 0.01; F = 14.6). In addition, there was interaction between the presentation order and the type of list presented (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recall order; Aging; Memory; Presentation order.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000019

Veratridine increases the survival of retinal ganglion cells in vitro
Provedor de dados: 56
Autores: Pereira,S.P.F.; Araujo,E.G.
Neuronal cell death is an important phenomenon involving many biochemical pathways. This degenerative event has been studied to understand how the cells activate the mechanisms that lead to self-destruction. Target cells and afferent cells play a relevant role in the regulation of natural cell death. We studied the effect of veratridine (1.5, 3.0, 4.5 and 6.0 µM) on the survival of neonatal rat retinal ganglion cells in vitro. Veratridine (3.0 µM), a well-known depolarizing agent that opens the Na+ channel, promoted a two-fold increase in the survival of retinal ganglion cells kept in culture for 48 h. This effect was dose-dependent and was blocked by 1.0 µM tetrodotoxin (a classical voltage-dependent Na+ channel blocker) and 30.0 µM flunarizine (a Na+ and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Veratridine; Retinal ganglion cells; Natural cell death; Development; Retina.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200014

Effects of caffeine on time to exhaustion in exercise performed below and above the anaerobic threshold
Provedor de dados: 56
Autores: Denadai,B.S.; Denadai,M.L.D.R.
Controversy still exists concerning the potential ergogenic benefit of caffeine (CAF) for exercise performance. The purpose of this study was to compare the effects of CAF ingestion on endurance performance during exercise on a bicycle ergometer at two different intensities, i.e., approximately 10% below and 10% above the anaerobic threshold (AT). Eight untrained males, non-regular consumers of CAF, participated in this study. AT, defined as the intensity (watts) corresponding to a lactate concentration of 4 mM, was determined during an incremental exercise test from rest to exhaustion on an electrically braked cycle ergometer. On the basis of these measurements, the subjects were asked to cycle until exhaustion at two different intensities, i.e.,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Caffeine; Anaerobic threshold; Endurance; Lactate; Perceived exertion.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400017

The prevalence of chronic diabetic complications and metabolic syndrome is not associated with maternal type 2 diabetes
Provedor de dados: 56
Autores: Scheffel,R.S.; Kramer,C.K.; Rados,D.V.; Pinto,L.C.; Crispim,D.; Gross,J.L.; Canani,L.H.
The maternal history of type 2 diabetes mellitus (DM) has been reported more frequently in patients with type 2 DM than paternal history. The aim of the present study was to determine if there was an association between maternal history of DM and the presence of chronic complications or metabolic syndrome (MetS) in patients with type 2 DM. A cross-sectional study was conducted with 1455 patients with type 2 DM. All outpatients with type 2 diabetes attending the endocrine clinics who fulfilled the eligibility criteria were included. Familial history of DM was determined with a questionnaire. Diabetic complications were assessed using standard procedures. The definition of MetS used was that of the World Health Organization and the National Cholesterol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Metabolic syndrome; Microvascular chronic complications; Diabetic retinopathy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200013

Changes in circulating endothelial progenitor cells predict responses of multiple myeloma patients to treatment with bortezomib and dexamethasone
Provedor de dados: 56
Autores: Wang,L.; Du,F.; Zhang,H.M.; Zhang,W.J.; Wang,H.X.
Four cycles of chemotherapy are required to assess responses of multiple myeloma (MM) patients. We investigated whether circulating endothelial progenitor cells (cEPCs) could be a biomarker for predicting patient response in the first cycle of chemotherapy with bortezomib and dexamethasone, so patients might avoid ineffective and costly treatments and reduce exposure to unwanted side effects. We measured cEPCs and stromal cell-derived factor-1α (SDF-1α) in 46 MM patients in the first cycle of treatment with bortezomib and dexamethasone, and investigated clinical relevance based on patient response after four 21-day cycles. The mononuclear cell fraction was analyzed for cEPC by FACS analysis, and SDF-1α was analyzed by ELISA. The study population was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemotherapy; Circulating endothelial progenitor cells; Multiple myeloma.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800736

In vivo and in vitro anti-inflammatory and anti-nociceptive activities of lovastatin in rodents
Provedor de dados: 56
Autores: Gonçalves,D.O.; Calou,I.B.F.; Siqueira,R.P.; Lopes,A.A.; Leal,L.K.A.; Brito,G.A.C.; Tomé,A.R.; Viana,G.S.B.
Statins are among the most prescribed drugs in recent clinical practice. They are also known for their pleiotropic actions, which are independent of their lipid-lowering properties. The effect of lovastatin was investigated against carrageenan-induced paw edema in male Wistar rats (200-250 g) and on leukocyte migration, as measured by carrageenan-induced peritonitis in male Swiss mice (20-25 g), which are models of acute inflammation. Lovastatin (administered 1 h prior to carrageenan), at oral doses of 2, 5, and 10 mg/kg, markedly attenuated paw edema formation in rats at the 4th hour after carrageenan injection (25, 43, and 37% inhibition, respectively). Inhibitions of 20, 45 and 80% were observed in the leukocyte migration, as evaluated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statin; Inflammation; Anti-nociception; Myeloperoxidase; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200011

Distribution of 131I-labeled Bothrops erythromelas venom in mice
Provedor de dados: 56
Autores: Vasconcelos,C.M.L.; Valença,R.C.; Araújo,E.A.; Modesto,J.C.A.; Pontes,M.M.; Brazil,T.K.; Guarnieri,M.C.
Bothrops erythromelas is responsible for many snake bites in northeastern Brazil. In the present study we determined the in vivo distribution of the venom following its subcutaneous injection into mice. B. erythromelas venom and albumin were labeled individually with 131I by the chloramine T method, and separated in a Sephacryl® S-200 column. The efficiency of labeling was 68%. Male Swiss mice (40-45 g), which had been provided with drinking water containing 0.05% KI over a period of 10 days prior to the experiment, were inoculated dorsally (sc) with 0.3 ml (2.35 x 105 cpm/mouse) of 131I-venom (N = 42), 131I-albumin or 131I (controls, N = 28 each). Thirty minutes and 1, 3, 6, 12, 18 and 24 h after inoculation, the animals were perfused with 0.85% NaCl and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops erythromelas; Snake venom; Protein iodination.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300017

Dexamethasone and sodium carboxymethyl cellulose prevent postoperative intraperitoneal adhesions in rats
Provedor de dados: 56
Autores: Du,X.H.; Liu,J.Q.; Xin,K.; Liu,G.H.
We aimed to evaluate the effects of the barrier agent sodium carboxymethyl cellulose (SCMC) with and without dexamethasone for the prevention of postoperative adhesion formation in a rat model of postoperative peritoneal adhesion. A total of 160 three-month old male and female Wistar rats underwent a laparotomy, and adhesions were induced by ileocecal abrasion. Rats were randomly assigned to 4 groups (n=40 each): group A, untreated; group B, treated with SCMC only; group C1, treated with SCMC + 3 mg dexamethasone, and group C2, treated with SCMC + 8 mg dexamethasone. After 12 days, adhesion formation and histopathological changes were compared. In groups A, B, C1, and C2, the mortality rates were 10, 5, 5, and 5%, respectively. In groups C1 and C2, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dexamethasone; Sodium carboxymethyl cellulose; Adhesions.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400344

Effect of D-alpha-tocopherol on tubular nephron acidification by rats with induced diabetes mellitus
Provedor de dados: 56
Autores: Nascimento Gomes,G.; Barbosa,F.T.; Radaeli,R.F.; Cavanal,M.F.; Mello Aires,M.; Zaladek Gil,F.
The objective of the present study was to determine if treatment of diabetic rats with D-alpha-tocopherol could prevent the changes in glomerular and tubular function commonly observed in this disease. Sixty male Wistar rats divided into four groups were studied: control (C), control treated with D-alpha-tocopherol (C + T), diabetic (D), and diabetic treated with D-alpha-tocopherol (D + T). Treatment with D-alpha-tocopherol (40 mg/kg every other day, ip) was started three days after diabetes induction with streptozotocin (60 mg/kg, ip). Renal function studies and microperfusion measurements were performed 30 days after diabetes induction and the kidneys were removed for morphometric analyses. Data are reported as means ± SEM. Glomerular filtration rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; D-alpha-tocopherol; Renal function; Nephropathy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700007

Pulmonary vein parameters are similar or better predictors than left atrial diameter for paroxysmal atrial fibrillation after cryoablation
Provedor de dados: 56
Autores: Li,Bolin; Ma,Honglan; Guo,Huihui; Liu,Peng; Wu,Yue; Fan,Lihong; Cao,Yumeng; Jian,Zhijie; Sun,Chaofeng; Li,Hongbing.
Left atrial diameter (LAD) has been considered an independent risk factor for atrial fibrillation (AF) relapse after pulmonary vein isolation (PVI). However, whether LAD or other factors are more predictive of late recurrence in patients with paroxysmal AF remains unclear. We aimed to evaluate the value of pulmonary vein (PV) parameters for predicting AF relapse 1 year after patients underwent cryoablation for paroxysmal AF. Ninety-seven patients with paroxysmal AF who underwent PVI successfully were included. PV parameters were measured through computed tomography scans prior to PVI. A total of 28 patients had recurrence of AF at one-year follow-up. The impact of several variables on recurrence was evaluated in multivariate analyses. LAD and the time from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atrial fibrillation; Cryoablation; Right inferior pulmonary vein; Pulmonary vein parameter.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900610

Effect of neoadjuvant chemotherapy on low-density lipoprotein (LDL) receptor and LDL receptor-related protein 1 (LRP-1) receptor in locally advanced breast cancer
Provedor de dados: 56
Autores: Pires,L.A.; Hegg,R.; Freitas,F.R.; Tavares,E.R.; Almeida,C.P.; Baracat,E.C.; Maranhão,R.C.
Low-density lipoprotein (LDL) receptors are overexpressed in most neoplastic cell lines and provide a mechanism for the internalization and concentration of drug-laden nanoemulsions that bind to these receptors. The aim of the present study was to determine whether the administration of standard chemotherapeutic schemes can alter the expression of LDL and LDL receptor-related protein 1 (LRP-1) receptors in breast carcinoma. Fragments of tumoral and normal breast tissue from 16 consecutive volunteer women with breast cancer in stage II or III were obtained from biopsies before the beginning of neoadjuvant chemotherapy and after chemotherapy, from fragments excised during mastectomy. Tissues were analyzed by immunohistochemistry for both receptors. Because...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Lipoproteins; LDL receptor; LRP-1 receptor; Chemotherapy; Nanoemulsion; Drug delivery.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600012

Hunting for differentially expressed genes
Provedor de dados: 56
Autores: Vedoy,C.G.; Bengtson,M.H.; Sogayar,M.C.
Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differential gene expression; Differential hybridization; Differential display/DDRT; Subtraction hybridization; Suppressive PCR; DNA microarrays/chips.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700012

Radioautographology: the proposal of a new concept
Provedor de dados: 56
Autores: Nagata,T.
A new concept termed "radioautographology" is advocated. This term was synthesized from "radioautography" and "ology", expressing a new science derived from radioautography. The concept of radioautographology (RAGology) is that of a science whose objective is to localize radioactive substances in the biological structure of objects and to analyze and study the significance of these substances in the biological structure. On the other hand, the old term radioautography (RAG) is the technique used to demonstrate the pattern of localization of various radiolabeled compounds in specimens. The specimens used in biology and medicine are cells and tissues. They are fixed, sectioned and placed in contact with the radioautographic emulsions, which are exposed and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Light microscopy; Electron microscopy; Radioautography; Technique; Application.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800001

Scintigraphy and Doppler ultrasonography for the evaluation of obstructive urinary calculi
Provedor de dados: 56
Autores: Gandolpho,L.; Sevillano,M.; Barbieri,A.; Ajzen,S.; Schor,N.; Ortiz,V.; Heilberg,I.P.
Forty-seven patients with unilateral obstructive calculi (12 males and 35 females) were submitted to 99mTc-diethylene triamine pentaacetic acid (DTPA) or 99mTc-dimercaptosuccinic acid (DMSA) scans for assessment of renal function. The scans revealed unilateral functional deficit in 68 and 66% of the patients, respectively. A calculus size of 1.1 to 2.0 cm was significantly associated with deficit detected by DTPA, but duration of obstruction and calculus localization were not. After relief of the obstruction, the mean percent renal function of the affected kidney was found to be significantly increased from 25 ± 12% to 29 ± 12% in DTPA and from 21 ± 15% to 24 ± 12% in DMSA. Initial Doppler ultrasonography performed in 35 patients detected an increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract obstruction; Obstructive calculi; DTPA and DMSA scans; Resistive index; Doppler ultrasonography.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600007

Ziyuglycoside II-induced apoptosis in human gastric carcinoma BGC-823 cells by regulating Bax/Bcl-2 expression and activating caspase-3 pathway
Provedor de dados: 56
Autores: Zhu,A.K.; Zhou,H.; Xia,J.Z.; Jin,H.C.; Wang,K.; Yan,J.; Zuo,J.B.; Zhu,X.; Shan,T.
Ziyuglycoside II is an active compound of Sanguisorba officinalis L. that has anti-inflammation, antioxidation, antibiosis, and homeostasis properties. We report here on the anticancer effect of ziyuglycoside II on human gastric carcinoma BGC-823 cells. We investigated the effects of ziyuglycoside II on cell growth, cell cycle, and cell apoptosis of this cell line. Our results revealed that ziyuglycoside II could inhibit the proliferation of BGC-823 cells by inducing apoptosis but not cell cycle arrest, which was associated with regulation of Bax/Bcl-2 expression, and activation of the caspase-3 pathway. Our study is the first to report the antitumor potential of ziyuglycoside II in BGC-823 gastric cancer cells. Ziyuglycoside II may become a potential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ziyuglycoside II; BGC-823 cells; Apoptosis; Bax/Bcl-2; Caspase-3.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800670

Antibodies against electronegative LDL inhibit atherosclerosis in LDLr-/- mice
Provedor de dados: 56
Autores: Grosso,D.M.; Ferderbar,S.; Wanschel,A.C.B.A.; Krieger,M.H.; Higushi,M.L.; Abdalla,D.S.P.
In order to determine the effect of antibodies against electronegative low-density lipoprotein LDL(-) on atherogenesis, five groups of LDL low receptor-deficient (LDLr-/-) mice (6 per group) were immunized with the following antibodies (100 µg each): mouse anti-LDL(-) monoclonal IgG2b, rabbit anti-LDL(-) polyclonal IgG or its Fab fragments and mouse irrelevant monoclonal IgG and non-immunized controls. Antibodies were administered intravenously one week before starting the hypercholesterolemic diet (1.25% cholesterol) and then every week for 21 days. The passive immunization with anti-LDL(-) monoclonal IgG2b, polyclonal antibody and its derived Fab significantly reduced the cross-sectional area of atherosclerotic lesions at the aortic root of LDLr-/- mice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minimally modified LDL; Passive immunization; Monoclonal antibody; Atherogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200007

TNNI3K is a novel mediator of myofilament function and phosphorylates cardiac troponin I
Provedor de dados: 56
Autores: Wang,Hui; Wang,Lin; Song,Li; Zhang,Yan-Wan; Ye,Jue; Xu,Rui-Xia; Shi,Na; Meng,Xian-Min.
The phosphorylation of cardiac troponin I (cTnI) plays an important role in the contractile dysfunction associated with heart failure. Human cardiac troponin I-interacting kinase (TNNI3K) is a novel cardiac-specific functional kinase that can bind to cTnI in a yeast two-hybrid screen. The purpose of this study was to investigate whether TNNI3K can phosphorylate cTnI at specific sites and to examine whether the phosphorylation of cTnI caused by TNNI3K can regulate cardiac myofilament contractile function. Co-immunoprecipitation was performed to confirm that TNNI3K could interact with cTnI. Kinase assays further indicated that TNNI3K did not phosphorylate cTnI at Ser23/24 and Ser44, but directly phosphorylated Ser43 and Thr143 in vitro. The results obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNNI3K; CTnI; Phosphorylation; Contraction function.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200128

Incidence of heart failure in infarcted rats that die spontaneously
Provedor de dados: 56
Autores: Flumignan,R.L.G.; Kanashiro,R.M.; Saraiva,R.M.; Portes,L.A.; Antonio,E.L.; Ishigai,M.M.S.; Tucci,P.J.F.
The present study reports for the first time the incidence of congestive heart failure (CHF) in previously infarcted rats that died spontaneously. Previously, pulmonary (PWC) and hepatic (HWC) water contents were determined in normal rats: 14 control animals were evaluated immediately after sacrifice, 8 placed in a refrigerator for 24 h, and 10 left at room temperature for 24 h. In the infarcted group, 9 rats died before (acute) and 28 died 48 h after (chronic) myocardial infarction. Thirteen chronic animals were submitted only to autopsy (N = 13), whereas PWC and HWC were also determined in the others (N = 15). Seven rats survived 48 h and died during anesthesia. Notably, PWC differed in normal rats: ambient (75.7 ± 1.3%) &lt; control (77.5 ± 0.7%) &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Myocardial infarction; Rats; Pulmonary congestion; Hepatic congestion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000008

Inflammatory response in a rat model of gastroschisis is associated with an increase of NF-kappaB
Provedor de dados: 56
Autores: Sbragia,L.; Schmidt,A.F.; Moraes,S.; Bittencourt,D.G.; Gonçalves,F.L.L.; Pereira,L.A.V.D.; Velloso,L.A.
Babies with gastroschisis have high morbidity, which is associated with inflammatory bowel injury caused by exposure to amniotic fluid. The objective of this study was to identify components of the inflammatory response in the intestine and liver in an experimental model of gastroschisis in rats. The model was surgically created at 18.5 days of gestation. The fetuses were exposed through a hysterotomy and an incision at the right of the umbilicus was made, exposing the fetal bowel. Then, the fetus was placed back into the uterus until term. The bowel in this model had macro- and microscopic characteristics similar to those observed in gastroschisis. The study was conducted on three groups of 20 fetuses each: gastroschisis, control, and sham fetuses. Fetal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroschisis; Inflammation; Inflammatory mediators; Nuclear factor kappaB.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200006

Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage
Provedor de dados: 56
Autores: Manfredini,V.; Roehrs,R.; Peralba,M.C.R.; Henriques,J.A.P.; Saffi,J.; Ramos,A.L.L.P.; Benfato,M.S.
Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1delta, sod2delta and the double mutant) were subjected to H2O2 stress in the stationary phase. The highest sensitivity was observed in the sod2delta mutant, while the sod1deltasod2delta double mutant was not sensitive. Sod mutants had lower catalase activity (44%) than wild-type cells, independent of H2O2 stress. Untreated cells of sod1deltasod2delta double mutants showed increased glutathione peroxidase activity (126%), while sod1delta had lower activity (77%) than the wild type. Glutathione levels in sod1delta were increased (200-260%) after exposure to various H2O2 concentrations. In addition, the highest malondialdehyde levels could be observed without H2O2 treatment in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Hydrogen peroxide; Saccharomyces cerevisiae; Reactive oxygen species.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200001

Inflammation and oxidative stress in heart failure: effects of exercise intensity and duration
Provedor de dados: 56
Autores: Ribeiro-Samora,G.A.; Rabelo,L.A.; Ferreira,A.C.C.; Favero,M.; Guedes,G.S.; Pereira,L.S.M.; Parreira,V.F.; Britto,R.R.
Although acute exercise is apparently pro-inflammatory and increases oxidative stress, it can promote the necessary stress stimulus to train chronic adaptations in patients with chronic heart failure (CHF). This study aimed to compare the effects of exercise intensity and duration on the inflammatory markers soluble tumor necrosis factor receptor (sTNFR1) and interleukin-6 (IL-6), and on oxidative stress [malondialdehyde (MDA) and antioxidant enzymes: catalase (CAT) and superoxide dismutase (SOD)] in individuals with CHF. Eighteen patients performed three exercise sessions: 30 min of moderate-intensity (M30) exercise, 30 min of low-intensity (L30) exercise, and 45 min of low-intensity (L45) exercise. Blood analysis was performed before exercise (baseline),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Inflammation; Oxidative stress; Exercise intensity; Exercise duration.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900608

Antagonism by hemoglobin of effects induced by L-arginine in neuromuscular preparations from rats
Provedor de dados: 56
Autores: Ambiel,C.R.; Alves-Do-Prado,Wilson.
Nitric oxide (NO)-synthase is present in diaphragm, phrenic nerve and vascular smooth muscle. It has been shown that the NO precursor L-arginine (L-Arg) at the presynaptic level increases the amplitude of muscular contraction (AMC) and induces tetanic fade when the muscle is indirectly stimulated at low and high frequencies, respectively. However, the precursor in muscle reduces AMC and maximal tetanic fade when the preparations are stimulated directly. In the present study the importance of NO synthesized in different tissues for the L-Arg-induced neuromuscular effects was investigated. Hemoglobin (50 nM) did not produce any neuromuscular effect, but antagonized the increase in AMC and tetanic fade induced by L-Arg (9.4 mM) in rat phrenic nerve-diaphragm...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skeletal muscle; Nitric oxide; Tetanic fade; L-arginine; D-arginine; Hemoglobin.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400017

Mapping and signaling of neural pathways involved in the regulation of hydromineral homeostasis
Provedor de dados: 56
Autores: Antunes-Rodrigues,J.; Ruginsk,S.G.; Mecawi,A.S.; Margatho,L.O.; Cruz,J.C.; Vilhena-Franco,T.; Reis,W.L.; Ventura,R.R.; Reis,L.C.; Vivas,L.M.; Elias,L.L.K.
Several forebrain and brainstem neurochemical circuitries interact with peripheral neural and humoral signals to collaboratively maintain both the volume and osmolality of extracellular fluids. Although much progress has been made over the past decades in the understanding of complex mechanisms underlying neuroendocrine control of hydromineral homeostasis, several issues still remain to be clarified. The use of techniques such as molecular biology, neuronal tracing, electrophysiology, immunohistochemistry, and microinfusions has significantly improved our ability to identify neuronal phenotypes and their signals, including those related to neuron-glia interactions. Accordingly, neurons have been shown to produce and release a large number of chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypothalamus; Gaseous neuromodulators; Neuropeptides; Endocannabinoids; Glial cells; Neurotransmitters.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400327

Melatonin and N-acetyl-serotonin cross the red blood cell membrane and evoke calcium mobilization in malarial parasites
Provedor de dados: 56
Autores: Hotta,C.T.; Markus,R.P.; Garcia,C.R.S.
The duration of the intraerythrocytic cycle of Plasmodium is a key factor in the pathogenicity of this parasite. The simultaneous attack of the host red blood cells by the parasites depends on the synchronicity of their development. Unraveling the signals at the basis of this synchronicity represents a challenging biological question and may be very important to develop alternative strategies for therapeutic approaches. Recently, we reported that the synchrony of Plasmodium is modulated by melatonin, a host hormone that is synthesized only during the dark phases. Here we report that N-acetyl-serotonin, a melatonin precursor, also releases Ca2+ from isolated P. chabaudi parasites at micro- and nanomolar concentrations and that the release is blocked by 250...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Plasmodium; Calcium; Melatonin; Circadian rhythm; Host-parasite interaction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100016

NMDA receptor blockade alters the intracellular distribution of neuronal nitric oxide synthase in the superficial layers of the rat superior colliculus
Provedor de dados: 56
Autores: Bittencourt-Navarrete,R.E. de; Nascimento,I.C.do; Santiago,M.F.; Mendez-Otero,R.
Nitric oxide (NO) is a molecular messenger involved in several events of synaptic plasticity in the central nervous system. Ca2+ influx through the N-methyl-D-aspartate receptor (NMDAR) triggers the synthesis of NO by activating the enzyme neuronal nitric oxide synthase (nNOS) in postsynaptic densities. Therefore, NMDAR and nNOS are part of the intricate scenario of postsynaptic densities. In the present study, we hypothesized that the intracellular distribution of nNOS in the neurons of superior colliculus (SC) superficial layers is an NMDAR activity-dependent process. We used osmotic minipumps to promote chronic blockade of the receptors with the pharmacological agent MK-801 in the SC of 7 adult rats. The effective blockade of NMDAR was assessed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superior colliculus; Nitric oxide synthase; NMDA receptor; Postsynaptic density targeting.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200007

Effects of isoflavone on the learning and memory of women in menopause: a double-blind placebo-controlled study
Provedor de dados: 56
Autores: Santos-Galduróz,R.F.; Galduróz,J.C.F.; Facco,R.L.; Hachul,H.; Tufik,S.
Hormone decline is common to all women during aging and, associated with other factors, leads to cognitive impairment. Its replacement enhances cognitive performance, but not all women present a clinical and family or personal history that justifies its use, mainly women with a history of cancer. The aim of this study was to determine whether a daily oral dose of 80 mg of isoflavone extract for 4 months can produce benefits in women with low hormone levels, contributing to improvement in cognitive aspects. The sample comprised 50- to 65-year-old women whose menstruation had ceased at least 1 year before and who had not undergone hormone replacement. The volunteers were allocated to two groups of 19 individuals each, i.e., isoflavone and placebo. There was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoflavone; Cognitive disorders; Aging; Information integration; Memory; Menopause.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100016

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways
Provedor de dados: 56
Autores: Leng,Xiaoyan; Shang,Jing; Gao,Danhui; Wu,Jiang.
Although the effects of low-intensity pulsed ultrasound (LIPUS) on diverse cell types have been fully studied, the functional role of LIPUS in keratinocytes remains poorly understood. This study aimed to investigate the effects of LIPUS on proliferation and migration of HaCaT cells as well as the regulatory mechanisms associated with signaling pathways. Human HaCaT cells were exposed or not to LIPUS, and cell proliferation and migration were measured by BrdU incorporation assay and Transwell assay, respectively. Expression of proteins associated with proliferation and migration was evaluated by western blot analysis. Expression of key kinases in the PI3K/AKT and JNK pathways was also evaluated by western blot analysis. Effects of LIPUS on the PI3K/AKT and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low-intensity pulsed ultrasound; HaCaT cells; Proliferation; Migration; PI3K/AKT/JNK.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200603

Postnatal development of rats exposed to fluoxetine or venlafaxine during the third week of pregnancy
Provedor de dados: 56
Autores: da-Silva,V.A.; Altenburg,S.P.; Malheiros,L.R.; Thomaz,T.G.; Lindsey,C.J.
The aim of the present study was to compare the toxic effects of fluoxetine (F) (8 and 16 mg/kg) and venlafaxine (V) (40 and 80 mg/kg) administered during the third week of pregnancy on early development of rats. Both antidepressants were administered by gavage on pregnancy days 15 to 20 to groups of 10 to 12 animals each. Duration of gestation, food and water consumption, number of live pups and birth weight were recorded. Litters were culled to six pups at birth (day 1) and followed for growth until weaning (day 25). On day 60, a male and a female from each litter were injected with the 5-HT1 agonist, 5-methoxy-N,N-dimethyltryptamine (6 mg/kg, ip) and the serotonergic syndrome was graded. Fluoxetine but not venlafaxine reduced the duration of pregnancy...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Development; Fluoxetine; Venlafaxine; Rats.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100014

PPARγ induces growth inhibition and apoptosis through upregulation of insulin-like growth factor-binding protein-3 in gastric cancer cells
Provedor de dados: 56
Autores: Kim,S.Y.; Kim,M.S.; Lee,M.K.; Kim,J.S.; Yi,H.K.; Nam,S.Y.; Lee,D.Y.; Hwang,P.H.
Peroxisome proliferator activator receptor-gamma (PPARγ) is a ligand-activated transcriptional factor involved in the carcinogenesis of various cancers. Insulin-like growth factor-binding protein-3 (IGFBP-3) is a tumor suppressor gene that has anti-apoptotic activity. The purpose of this study was to investigate the anticancer mechanism of PPARγ with respect to IGFBP-3. PPARγ was overexpressed in SNU-668 gastric cancer cells using an adenovirus gene transfer system. The cells in which PPARγ was overexpressed exhibited growth inhibition, induction of apoptosis, and a significant increase in IGFBP-3 expression. We investigated the underlying molecular mechanisms of PPARγ in SNU-668 cells using an IGFBP-3 promoter/luciferase reporter system. Luciferase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PPARγ; IGFBP-3; Apoptosis; Gastric cancer.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300226

Correlations between forced oscillation technique parameters and pulmonary densitovolumetry values in patients with acromegaly
Provedor de dados: 56
Autores: Camilo,G.B.; Carvalho,A.R.S.; Machado,D.C.; Mogami,R.; Kasuki,L.; Gadelha,M.R.; Melo,P.L.; Lopes,A.J.
The aims of this study were to evaluate the forced oscillation technique (FOT) and pulmonary densitovolumetry in acromegalic patients and to examine the correlations between these findings. In this cross-sectional study, 29 non-smoking acromegalic patients and 17 paired controls were subjected to the FOT and quantification of lung volume using multidetector computed tomography (Q-MDCT). Compared with the controls, the acromegalic patients had a higher value for resonance frequency [15.3 (10.9-19.7) vs 11.4 (9.05-17.6) Hz, P=0.023] and a lower value for mean reactance [0.32 (0.21-0.64) vs 0.49 (0.34-0.96) cm H2O/L/s2, P=0.005]. In inspiratory Q-MDCT, the acromegalic patients had higher percentages of total lung volume (TLV) for nonaerated and poorly aerated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acromegaly; Tomography; Respiratory function tests; Forced oscillation technique.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000003

Comparative pathogenicity of Coxsackievirus A16 circulating and noncirculating strains in vitro and in a neonatal mouse model
Provedor de dados: 56
Autores: Huang,L.; Liu,X.; Li,J.L.; Chang,J.L.; Liu,G.C.; Yu,X.F.; Zhang,W.Y.
An enterovirus 71 (EV71) vaccine for the prevention of hand, foot, and mouth disease (HMFD) is available, but it is not known whether the EV71 vaccine cross-protects against Coxsackievirus (CV) infection. Furthermore, although an inactivated circulating CVA16 Changchun 024 (CC024) strain vaccine candidate is effective in newborn mice, the CC024 strain causes severe lesions in muscle and lung tissues. Therefore, an effective CV vaccine with improved pathogenic safety is needed. The aim of this study was to evaluate the in vivo safety and in vitro replication capability of a noncirculating CVA16 SHZH05 strain. The replication capacity of circulating CVA16 strains CC024, CC045, CC090 and CC163 and the noncirculating SHZH05 strain was evaluated by cytopathic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coxsackievirus A16; Pathogenicity; Circulating strain; Non-circulating strain.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500420

Detection of somatic mutations of the PIG-A gene in Brazilian patients with paroxysmal nocturnal hemoglobinuria
Provedor de dados: 56
Autores: Franco de Carvalho,R.; Arruda,V.R.; Saad,S.T.O.; Costa,F.F.
Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired clonal syndrome characterized by intravascular hemolysis mediated by complement, thrombotic events and alterations in hematopoiesis. Basically, the molecular events which underlie the complexity of the syndrome consist of the absence of the glycosylphosphatidylinositol (GPI) anchor as a consequence of somatic mutations in the PIG-A gene, located on the X chromosome. The GPI group is responsible for the attachment of many proteins to the cytoplasmic membrane. Two of them, CD55 and CD59, have a major role in the inhibition of the action of complement on the cellular membrane of blood cells. The absence of GPI biosynthesis can lead to PNH. Since mutations in the PIG-A gene are always present in patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paroxysmal nocturnal hemoglobinuria; Intravascular hemolysis; Complement PIG-A; Mutation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600010

Familial hyperaldosteronism
Provedor de dados: 56
Autores: Torpy,D.J.; Stratakis,C.A.; Chrousos,G.P.
Aldosterone, the major circulating mineralocorticoid, participates in blood volume and serum potassium homeostasis. Primary aldosteronism is a disorder characterised by hypertension and hypokalaemia due to autonomous aldosterone secretion from the adrenocortical zona glomerulosa. Improved screening techniques, particularly application of the plasma aldosterone:plasma renin activity ratio, have led to a suggestion that primary aldosteronism may be more common than previously appreciated among adults with hypertension. Glucocorticoid-remediable aldosteronism (GRA) was the first described familial form of hyperaldosteronism. The disorder is characterised by aldosterone secretory function regulated chronically by ACTH. Hence, aldosterone hypersecretion can be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aldosterone; Hyperaldosteronism; Genetics; Adrenal tumour; Hypertension.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000004

The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis
Provedor de dados: 56
Autores: Wang,Z.R.; Wang,J.H.; Hu,C.L.; Cao,W.G.; Shen,X.J.; Wu,M.Y.; Shen,L.; Wu,S.L.
Searching for effective Smad3 gene-based gene therapies for hepatic fibrosis, we constructed siRNA expression plasmids targeting the rat Smad3 gene and then delivered these plasmids into hepatic stellate cells (HSCs). The effect of siRNAs on the mRNA levels of Smad2, Smad3, Smad4, and collagens I-α1, III-α1 and IV-α1 (Colα1, Col3α1, Col4α1, respectively) was determined by RT-PCR. Eighty adult male Sprague-Dawley rats were randomly divided into three groups. Twice a week for 8 weeks, the untreated hepatic fibrosis model (N = 30) and the treated group (N = 20) were injected subcutaneously with 40% (v/v) carbon tetrachloride (CCl4)-olive oil (3 mL/kg), and the normal control group (N = 30) was injected with olive oil (3 mL/kg). In the 4th week, the treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smad3; SiRNA; Hepatic fibrosis; Carbon tetrachloride.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200002

The DNA puff BhB10-1 gene is differentially expressed in various tissues of Bradysia hygida late larvae and constitutively transcribed in transgenic Drosophila
Provedor de dados: 56
Autores: Monesi,N.; Sousa,J.F.; Paçó-Larson,M.L.
We extended the characterization of the DNA puff BhB10-1 gene of Bradysia hygida by showing that, although its mRNA is detected only at the end of the fourth larval instar, BhB10-1 expression is not restricted to the salivary gland, the tissue in which this gene is amplified. Different amounts of BhB10-1 mRNA were detected in other larval tissues such as gut, Malpighian tubules, fat body, brain and cuticle, suggesting that this gene is expressed differentially in the various tissues analyzed. Analysis of transgenic Drosophila carrying the BhB10-1 transcription unit and flanking sequences revealed that the tested fragment promotes transcription in a constitutive manner. We suggest that either cis-regulatory elements are missing in the transgene or factors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA puff; Gene expression; Development; Ecdysone; Transgenic Drosophila.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700003

Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells
Provedor de dados: 56
Autores: Zhang,L.; Liang,H.; Cao,W.; Xu,R.; Ju,X.L.
Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroblastoma; Survivin; Apoptosis; Invasion; RNA interference.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700548

Histone demethylase retinoblastoma binding protein 2 regulates the expression of α-smooth muscle actin and vimentin in cirrhotic livers
Provedor de dados: 56
Autores: Wang,Q.; Wang,L.X.; Zeng,J.P.; Liu,X.J.; Liang,X.M.; Zhou,Y.B.
Liver cirrhosis is one of the most common diseases of Chinese patients. Herein, we report the high expression of a newly identified histone 3 lysine 4 demethylase, retinoblastoma binding protein 2 (RBP2), and its role in liver cirrhosis in humans. The siRNA knockdown of RBP2 expression in hepatic stellate cells (HSCs) reduced levels of α-smooth muscle actin (α-SMA) and vimentin and decreased the proliferation of HSCs; and overexpression of RBP2 increased α-SMA and vimentin levels. Treatment with transforming growth factor β (TGF-β) upregulated the expression of RBP2, α-SMA, and vimentin, and the siRNA knockdown of RBP2 expression attenuated TGF-β-mediated upregulation of α-SMA and vimentin expression and HSC proliferation. Furthermore, RBP2 was highly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RBP2; Liver cirrhosis; Fibrosis; Α-SMA; Vimentin; HSC.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900739

Release of plasma atrial natriuretic peptide after volume expansion is not related to pituitary-adrenal axis diurnal variation in normal subjects
Provedor de dados: 56
The existence of a circadian rhythm of atrial natriuretic peptide (ANP) in humans is controversial. We studied the plasma ANP response to isotonic blood volume expansion in the morning and in the afternoon and its relationship with adrenocorticotropic hormone (ACTH)-cortisol diurnal variation in seven normal subjects. Basal plasma ANP level was similar in the morning (19.6 ± 2.4 pg/ml) and in the afternoon (21.8 ± 4.8 pg/ml). The ANP peak obtained with saline infusion (0.9% NaCl, 12 ml/kg) in the morning (49.4 ± 8 pg/ml) did not differ from that obtained in the afternoon (60.3 ± 10.1 pg/ml). There was no correlation between the individual mean cortisol and ACTH levels and the ANP peak obtained with saline infusion. These data indicate no diurnal variation...
Palavras-chave: ANP; Diurnal variation; ACTH; Cortisol.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500008

Na,K-ATPase: a molecular target for Leptospira interrogans endotoxin
Provedor de dados: 56
On the basis of our report that a glycolipoprotein fraction (GLP) extracted from Leptospira interrogans contains a potent inhibitor of renal Na,K-ATPase, we proposed that GLP-induced inhibition of Na,K-ATPase might be the primary cellular defect in the physiopathology of leptospirosis. The present study was designed to test this hypothesis by determining whether or not 1) GLP inhibits all the isoforms of Na,K-ATPase which are expressed in the tissues affected by leptospirosis, 2) Na,K-ATPase from leptospirosis-resistant species, such as the rat, is sensitive to GLP, 3) GLP inhibits Na,K-ATPase from intact cells, and 4) GLP inhibits ouabain-sensitive H,K-ATPase. The results indicate that in the rabbit, a leptospirosis-sensitive species, GLP inhibits with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Na/K-ATPase; H/K-ATPase; Na/K-ATPase isoforms; Rubidium transport; Leptospirosis; Ouabain.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200009

Evidence for the involvement of peripheral β-adrenoceptors in delayed liquid gastric emptying induced by dipyrone, 4-aminoantipyrine, and antipyrine in rats
Provedor de dados: 56
Autores: Vinagre,A.M.; Collares,E.F.
Dipyrone (Dp), 4-aminoantipyrine (AA), and antipyrine (At) delay liquid gastric emptying (GE) in rats. We evaluated adrenergic participation in this phenomenon in a study in male Wistar rats (250-300 g) pretreated subcutaneously with guanethidine (GUA), 100 mg·kg−1·day−1, or vehicle (V) for 2 days before experimental treatments. Other groups of animals were pretreated intravenously (iv) 15 min before treatment with V, prazosin (PRA; 1 mg/kg), yohimbine (YOH; 3 mg/kg), or propranolol (PRO; 4 mg/kg), or with intracerebroventricular (icv) administration of 25 µg PRO or V. The groups were treated iv with saline or with 240 µmol/kg Dp, AA, or At. GE was determined 10 min later by measuring the percentage of gastric retention (%GR) of saline labeled with phenol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dipyrone; 4-Aminoantipyrine; Antipyrine; Sympathetic nervous system; Adrenergic receptors.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900735

Peripheral brain-derived neurotrophic factor is related to cardiovascular risk factors in active and inactive elderly men
Provedor de dados: 56
Autores: Zembron-Lacny,A.; Dziubek,W.; Rynkiewicz,M.; Morawin,B.; Woźniewski,M.
Regular exercise plays an important preventive and therapeutic role in heart and vascular diseases, and beneficially affects brain function. In blood, the effects of exercise appear to be very complex and could include protection of vascular endothelial cells via neurotrophic factors and decreased oxidative stress. The purpose of this study was to identify the age-related changes in peripheral brain-derived neurotrophic factor (BDNF) and its relationship to oxidative damage and conventional cardiovascular disease (CVD) biomarkers, such as atherogenic index, C-reactive protein (hsCRP) and oxidized LDL (oxLDL), in active and inactive men. Seventeen elderly males (61-80 years) and 17 young males (20-24 years) participated in this study. According to the 6-min...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurotrophins; Lipoproteins; Oxidative damage; Aging; Cardiorespiratory fitness.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700603

Glutamatergic transmission in the nucleus tractus solitarii: from server to peripherals in the cardiovascular information superhighway
Provedor de dados: 56
Afferent nerves carrying signals from mechanoreceptors in the aortic arch and carotid sinus terminate predominantly in the nucleus tractus solitarii (NTS). Signal transduction and neurotransmission in the NTS are critical for central cardiovascular reflex control, but little was known about either until the late 1970's. None of the numerous neuroactive chemicals found in the NTS had met strict criteria as a neurotransmitter in the baroreflex arc until data suggested that the excitatory amino acid L-glutamate (GLU) might be released from baroreceptor afferent terminals in the NTS. In anesthetized animals microinjection into the NTS of GLU, which can be demonstrated in terminals in the NTS, produces cardiovascular responses like those seen with activation of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glutamate; Nucleus tractus solitarii; Baroreceptor reflex; Cardiovascular; Vagus; Mechanoreceptors.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100001

Changes in expression of BDNF and its receptors TrkB and p75NTR in the hippocampus of a dog model of chronic alcoholism and abstinence
Provedor de dados: 56
Autores: Xu,R.; Duan,S.R.; Zhao,J.W.; Wang,C.Y.
Chronic ethanol consumption can produce learning and memory deficits. Brain-derived neurotrophic factor (BDNF) and its receptors affect the pathogenesis of alcoholism. In this study, we examined the expression of BDNF, tropomyosin receptor kinase B (TrkB) and p75 neurotrophin receptor (p75NTR) in the hippocampus of a dog model of chronic alcoholism and abstinence. Twenty domestic dogs (9-10 months old, 15-20 kg; 10 males and 10 females) were obtained from Harbin Medical University. A stable alcoholism model was established through ad libitum feeding, and anti-alcohol drug treatment (Zhong Yao Jie Jiu Ling, the main ingredient was the stems of watermelon; developed in our laboratory), at low- and high-doses, was carried out. The Zhong Yao Jie Jiu Ling was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic alcoholism; Brain-derived neurotrophic factor; Tropomyosin receptor kinase B; P75 Neurotrophin receptor; Immunohistochemistry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800703

Purification, partial characterization and role in lipid transport to developing oocytes of a novel lipophorin from the cowpea weevil, Callosobruchus maculatus
Provedor de dados: 56
Autores: Ximenes,A.A; Oliveira,G.A; Bittencourt-Cunha,P; Tomokyo,M; Leite,D.B; Folly,E; Golodne,D.M; Atella,G.C.
Lipid transport in arthropods is achieved by highly specialized lipoproteins, which resemble those described in vertebrate blood. Here we describe purification and characterization of the lipid-apolipoprotein complex, lipophorin (Lp), from adults and larvae of the cowpea weevil Callosobruchus maculatus. We also describe the Lp-mediated lipid transfer to developing oocytes. Lps were isolated from homogenates of C. maculatus larvae and adults by potassio bromide gradient and characterized with respect to physicochemical properties and lipid content. The weevil Lp (465 kDa) and larval Lp (585 kDa), with hydrated densities of 1.22 and 1.14 g/mL, contained 34 and 56% lipids and 9 and 7% carbohydrates, respectively. In both Lps, mannose was the predominant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callosobruchus maculatus; Lipophorin; Lipid transport; Oogenesis; Hydrocarbons.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100004

Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties
Provedor de dados: 56
Autores: da Rosa,H.S.; Salgueiro,A.C.F.; Colpo,A.Z.C.; Paula,F.R.; Mendez,A.S.L.; Folmer,V.
Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sida tuberculata; 20-hydroxyecdysone; Toxicity prediction; Antioxidant.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800602

Interaction between paraventricular nucleus and septal area in the control of physiological responses induced by angiotensin II
Provedor de dados: 56
Autores: Camargo,L.A.A.; Saad,W.A.; Simões,S.; Santos,T.A.B.; Saad,W. Abrão.
We determined the effects of losartan (40 nmol) and PD 123319 (40 nmol) (both non-peptides and selective antagonists of the AT1 and AT2 angiotensin receptors, respectively), and [Sar¹, Ala8] angiotensin II (ANG II) (40 nmol) (a non-selective peptide antagonist of angiotensin receptors) injected into the paraventricular nucleus (PVN) on the water and salt appetite, diuresis and natriuresis and mean arterial pressure (MAP) induced by administration of 10 nmol of ANG II into the medial septal area (MSA) of male Holtzman rats weighing 250-300 g. The volume of drug solution injected was 0.5 µl over a period of 10-15 s. The responses were measured over a period of 120 min. ANG II alone injected into the MSA induced an increase in all the above parameters (8.1 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AT1 receptors; AT2 receptors; Water; Sodium; Paraventricular nucleus; Medial septal area.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900002

Effect of aerobic training on EEG alpha asymmetry and depressive symptoms in the elderly: a 1-year follow-up study
Provedor de dados: 56
Autores: Deslandes,A.C.; Moraes,H.; Alves,H.; Pompeu,F.A.M.S.; Silveira,H.; Mouta,R.; Arcoverde,C.; Ribeiro,P.; Cagy,M.; Piedade,R.A.M.; Laks,J.; Coutinho,E.S.F.
The effect of physical exercise on the treatment of depressive elderly adults has not been investigated thus far in terms of changes in cortical hemispheric activity. The objective of the present study was to identify changes in depressive symptoms, quality of life, and cortical asymmetry produced by aerobic activity. Elderly subjects with a diagnosis of major depressive disorder (DSM-IV) were included. Twenty patients (70% females, 71 ± 3 years) were divided into an exercise group (pharmacological treatment plus aerobic training) and a control group (undergoing pharmacological treatment) in a quasi-experimental design. Pharmacological treatment was maintained stable throughout the study (antidepressants and anxiolytics). Subjects were evaluated by...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Alpha asymmetry; Quality of life; Depressive symptoms; Physical activity; Aerobic training; Major depression.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600010

Radioactive fucose as a tool for studying glycoprotein secretion
Provedor de dados: 56
Autores: Haddad,A.
The efficiency and reliability of radioactive fucose as a specific label for newly synthesized glycoproteins were investigated. Young adult male rabbits were injected intravitreally with [3H]-fucose, [3H]-galactose, [3H]-mannose, N-acetyl-[3H]-glucosamine or N-acetyl-[3H]-mannosamine, and killed 40 h after injection. In another series of experiments rabbits were injected with either [3H]-fucose or several tritiated amino acids and the specific activity of the vitreous proteins was determined. Vitreous samples were also processed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and histological sections of retina, ciliary body and lens (the eye components around the vitreous body) were processed for radioautography. The specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycoprotein; Fucose; Radioautography; Secretion; Vitreous body.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200004

Egocentric and allocentric spatial memory in healthy aging: performance on real-world tasks
Provedor de dados: 56
Autores: Fernandez-Baizan,C.; Diaz-Caceres,E.; Arias,J.L.; Mendez,M.
Although normal aging has been related to several cognitive difficulties, other processes have been studied less, such as spatial memory. Our aim was to compare egocentric and allocentric memory in an elderly population using ecological tasks. Twenty-eight cognitively unimpaired participants performed Egocentric and Allocentric Spatial Memory Tasks, as well as Spatial Span from CANTAB, Benton's Judge of Line Orientation test (JoLO), and Montreal Cognitive Assessment test (MoCA). The results revealed that younger participants showed better performance than older participants on both the Egocentric and Allocentric Spatial Memory Tasks, although only the Egocentric test was able to discriminate between younger, middle, and older elderly participants. Learning...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Egocentric spatial memory; Allocentric spatial memory; Aging; Spatial learning; Spatial strategies.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400611

Effects of resistance training protocols on nonlinear analysis of heart rate variability in metabolic syndrome
Provedor de dados: 56
Autores: Turri-Silva,N.; Garner,D.M.; Moosavi,S.H.; Ricci-Vitor,A.L.; Christofaro,D.G.D.; Netto Junior,J.; Vanzella,L.M.; Vanderlei,L.C.M.
Despite the various standard non-linear measurements used in autonomic modulation (AM) assessments usually being applied to long time-series, such analyses can sometimes be applied to shorter term series. To overcome this disadvantage, chaotic global methods were formulated by putting together heart rate variability (HRV) linear methods. Chaos provides information about vegetative function control related to cardiovascular risks. Applying this method can be useful to investigate the complexity of the health condition after resistance training protocols, as a therapeutic intervention in AM in metabolic syndrome individuals (MetS). This study aimed to compare the effects of two resistance training programs (conventional vs functional) in MetS using nonlinear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic modulation; Nonlinear dynamics; Metabolic syndrome; Resistance training; Exercise.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800610

Adrenocortical tumors in children
Provedor de dados: 56
Autores: Ribeiro,R.C.; Michalkiewicz,E.L.; Figueiredo,B.C.; DeLacerda,L.; Sandrini,F.; Pianovsky,M.D.; Sampaio,G.; Sandrini,R.
Childhood adrenocortical tumors (ACT) are rare. In the USA, only about 25 new cases occur each year. In Southern Brazil, however, approximately 10 times that many cases are diagnosed each year. Most cases occur in the contiguous states of São Paulo and Paraná. The cause of this higher rate has not been identified. Familial genetic predisposition to cancer (p53 mutations) and selected genetic syndromes (Beckwith-Wiedemann syndrome) have been associated with childhood ACT in general but not with the Brazilian counterpart. Most of the affected children are young girls with classic endocrine syndromes (virilizing and/or Cushing). Levels of urinary 17-ketosteroids and plasma dehydroepiandrosterone sulfate (DHEA-S), which are abnormal in approximately 90% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenocortical adenoma; Adrenocortical carcinoma; Adrenal gland disorders; Pediatric cancers; Childhood adrenocortical tumors.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000013

Association between tumor necrosis factor alpha rs1800629 polymorphism and risk of osteoarthritis in a Chinese population
Provedor de dados: 56
Autores: Chen,Jie; Wu,Yu; Yu,Jiannong; Shen,Jinming.
Osteoarthritis (OA) is the most common degenerative disease affecting articular cartilage. Some studies indicate that tumor necrosis factor alpha (TNF-α) gene rs1800629 polymorphism was associated with OA risk among Caucasian populations. To examine the role of this candidate gene in Asian populations, we conducted a hospital-based case-control study involving 257 knee OA patients and 305 controls in a Chinese population. Genotyping was performed using a custom-by-design 48-Plex single nucleotide polymorphism (SNP) Scan™ kit. Our study indicated that the AA genotype of TNF-α rs1800629 polymorphism was associated with increased risk of OA. Subsequently, we conducted a meta-analysis and found that rs1800629 polymorphism increased the risk of OA in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF-α; Osteoarthritis; Polymorphism; Meta-analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800604

Accuracy and prognostic ability of the SARC-F questionnaire and Ishii's score in the screening of sarcopenia in geriatric inpatients
Provedor de dados: 56
Autores: Li,Min; Kong,Yan; Chen,Hongcun; Chu,Aiqin; Song,Guiqi; Cui,Yan.
Sarcopenia remains poorly managed in clinical practice due to the lack of simple and accurate screening tools. This study aimed to identify the cutoff values of the SARC-F questionnaire and Ishii's score using the variables age, grip strength, and calf circumference in older inpatients in China to compare the accuracy of the two methods and to explore their predictive ability for adverse outcomes (rehospitalization, falls, fracture, and death). Hospitalized patients (n=138) aged ≥60 years were included. The accuracy of the two tools was evaluated using the reference diagnosis recommended by the Asian Working Group on Sarcopenia (assessing patients with measurements of muscle mass, handgrip strength, and usual gait speed). Follow-up data were obtained by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sarcopenia; Muscle mass; Geriatric inpatients; Screening; Score.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900606

Prediction of exposed domains of envelope glycoprotein in Indian HIV-1 isolates and experimental confirmation of their immunogenicity in humans
Provedor de dados: 56
Autores: Mohabatkar,H.; Kar,S.K.
We describe the impact of subtype differences on the seroreactivity of linear antigenic epitopes in envelope glycoprotein of HIV-1 isolates from different geographical locations. By computer analysis, we predicted potential antigenic sites of envelope glycoprotein (gp120 and gp4l) of this virus. For this purpose, after fetching sequences of proteins of interest from data banks, values of hydrophilicity, flexibility, accessibility, inverted hydrophobicity, and secondary structure were considered. We identified several potential antigenic epitopes in a B subtype strain of envelope glycoprotein of HIV-1 (IIIB). Solid- phase peptide synthesis methods of Merrifield and Fmoc chemistry were used for synthesizing peptides. These synthetic peptides corresponded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human immunodeficiency virus; Glycoprotein; Epitope; Immunogenicity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500008

No evidence for mutations in exons 1, 8 and 18 of the patched gene in sporadic skin lesions of Brazilian patients
Provedor de dados: 56
Autores: Granja,F.; Santarosa,P.L.; Leite,J.L.A.A.P.; Ward,L.S.
There is strong evidence that the patched (PTCH) gene is a gene for susceptibility to the nevoid basal cell carcinoma syndrome. PTCH has also been shown to mutate in both familial and sporadic basal cell carcinomas. However, mutations of the gene seem to be rare in squamous cell carcinomas. In order to characterize the role of the gene in the broader spectrum of sporadic skin malignant and pre-malignant lesions, we performed a polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis of genomic DNA extracted from 105 adult patients (46 females and 59 males). There were 66 patients with basal cell carcinomas, 30 with squamous cell carcinomas, 2 with malignant melanomas and 7 patients with precancerous lesions. Two tissue samples...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skin; Cancer; PTCH; Mutation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400006

Chlorpheniramine impairs functional recovery in Carassius auratus after telencephalic ablation
Provedor de dados: 56
Autores: Garção,D.C.; Canto-de-Souza,L.; Romaguera,F.; Mattioli,R.
We determined the effect of an H1 receptor antagonist on the functional recovery of Carassius auratus submitted to telencephalic ablation. Five days after surgery the fish underwent a spatial-choice learning paradigm test. The fish, weighing 6-12 g, were divided into four groups: telencephalic ablation (A) or sham lesion (S) and saline (SAL) or chlorpheniramine (CPA, ip, 16 mg/kg). For eight consecutive days each animal was trained individually in sessions separated by 24 h (alternate days). Training trials (T1-T8) consisted of finding the food in one of the feeders, which were randomly blocked for each subject. Animals received an intraperitoneal injection of SAL or CPA 10 min after the training trials. The time spent by the animals in each group to find...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional recovery; Spatial-choice learning; Histamine; Chlorpheniramine; Carassius auratus; Telencephalic ablation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400010

Evidence of the effect of dipyrone on the central nervous system as a determinant of delayed gastric emptying observed in rats after its administration
Provedor de dados: 56
Autores: Collares,E.F.; Vinagre,A.M.
Dipyrone administered intravenously (iv) delays gastric emptying (GE) in rats. The objectives of the present study were to assess: 1) the effect of the dose of dipyrone and time after its iv administration on GE in rats, 2) the effect of subdiaphragmatic vagotomy (VgX) and bilateral electrolytic lesion of the paraventricular nucleus (PVNX) on the delayed GE induced by the drug, and 3) the intracerebroventricular (icv) action of dipyrone and of one of its metabolites, 4-aminoantipyrine on GE. Male Wistar rats received saline labeled with phenol red intragastrically as a test meal. GE was indirectly assessed by the determination of percent gastric retention (GR) of the test meal 10 min after administration by gavage. Dipyrone delays GE in a dose- and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dipyrone; Paraventricular nucleus; Vagus nerve.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000014

Hyperalgesic effect induced by barbiturates, midazolam and ethanol: pharmacological evidence for GABA-A receptor involvement
Provedor de dados: 56
The involvement of GABA-A receptors in the control of nociception was studied using the tail-flick test in rats. Non-hypnotic doses of the barbiturates phenobarbital (5-50 mg/kg), pentobarbital (17-33 mg/kg), and thiopental (7.5-30 mg/kg), of the benzodiazepine midazolam (10 mg/kg) or of ethanol (0.4-1.6 g/kg) administered by the systemic route reduced the latency for the tail-flick response, thus inducing a 'hyperalgesic' state in the animals. In contrast, non-convulsant doses of the GABA-A antagonist picrotoxin (0.12-1.0 mg/kg) administered systemically induced an increase in the latency for the tail-flick response, therefore characterizing an 'antinociceptive' state. Previous picrotoxin (0.12 mg/kg) treatment abolished the hyperalgesic state induced by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Barbiturates; Midazolam; Ethanol; Picrotoxin; Hyperalgesia; GABA-A receptor.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200015

Regulation of T cell response to leishmania antigens by determinants of histocompatibility leukocyte class I and II molecules
Provedor de dados: 56
Autores: Bacellar,O.; Russo,C.; Carvalho,E.M.
It has been shown that HLA class I molecules play a significant role in the regulation of the proliferation of T cells activated by mitogens and antigens. We evaluated the ability of mAb to a framework determinant of HLA class I molecules to regulate T cell proliferation and interferon gamma (IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;) production against leishmania, PPD, C. albicans and tetanus toxoid antigens in patients with tegumentary leishmaniasis and healthy subjects. The anti-major histocompatibility complex (MHC) mAb (W6/32) suppressed lymphocyte proliferation by 90% in cultures stimulated with &lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;CD3, but the suppression was variable in cultures stimulated with leishmania antigen. This suppression ranged from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA class I tegumentary leishmaniasis modulation of T cell response by HLA class I interferon-&lt; FONT FACE=Symbol&gt; G&lt; /FONT&gt; And HLA class I soluble antigen and HLA class I.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200010

Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians
Provedor de dados: 56
Autores: Souza,D.R.S.; De Godoy,M.R.; Hotta,J.; Tajara,E.H.; Brandão,A.C.; Pinheiro Júnior,S.; Tognola,W.A.; Dos Santos,J.E.
The genetic basis for dementias is complex. A common polymorphism in the apolipoprotein E (APOE) gene is considered to be the major risk factor in families with sporadic and late-onset Alzheimer's disease as well as in the general population. The distribution of alleles and genotypes of the APOE gene in late-onset Alzheimer's disease (N = 68), other late-life dementias (N = 39), and in cognitively normal controls (N = 58) was determined, as also was the risk for Alzheimer's disease associated with the epsilon4 allele. Peripheral blood samples were obtained from a total of 165 individuals living in Brazil aged 65-82 years. Genomic DNA was amplified by the polymerase chain reaction and the products were digested with HhaI restriction enzyme. APOE epsilon2...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Alzheimer's disease; Vascular dementia; Dementia; Apolipoprotein E; Aging; Genetic polymorphisms.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700013

The effect of epidural and general anesthesia on newborn rectal temperature at elective cesarean section
Provedor de dados: 56
Autores: Yentur,E.A.; Topcu,I.; Ekici,Z.; Ozturk,T.; Keles,G.T.; Civi,M.
Both epidural and general anesthesia can impair thermoregulatory mechanisms during surgery. However, there is lack of information about the effects of different methods of anesthesia on newborn temperature. The purpose of this study was to determine whether there are differences in newborn rectal temperature related to type of anesthesia. Sixty-three pregnant women were randomly assigned to receive general or epidural anesthesia. Maternal core temperature was measured three times with a rectal probe just before anesthesia, at the beginning of surgery and at delivery. In addition, umbilical vein blood was sampled for pH. The rectal temperatures of the babies were recorded immediately after delivery, and Apgar scores were determined 1, 5, and 10 min after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rectal temperature; Newborn; Epidural anesthesia; General anesthesia; Cesarean delivery.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900014

Protective effects of fentanyl preconditioning on cardiomyocyte apoptosis induced by ischemia-reperfusion in rats
Provedor de dados: 56
Autores: Xu,Q.; Li,Q.-G.; Fan,G.-R.; Liu,Q.-H.; Mi,F.-L.; Liu,B.
We aimed to study the effect of fentanyl (Fen) preconditioning on cardiomyocyte apoptosis induced by ischemia-reperfusion (I/R) in rats. A total of 120 Sprague Dawley male rats (age: 3 months) were randomly divided into: sham operation group (S group), I/R group, normal saline I/R group (NS group), and fentanyl low, middle, and high dose groups (Fen1: 2 μg/kg; Fen2: 4 μg/kg; Fen3: 6 μg/kg). Heart rate (HR), mean arterial pressure (MAP), left ventricular developed pressure (LVDP), ±dp/dtmax, malondialdehyde (MDA), superoxide dismutase (SOD) activity, creatine phosphokinase-MB (CK-MB), and cardiac troponin-I (cTnI) were measured. Myocardial ischemic (MI) area, total apoptotic myocardial cells, and protein and mRNA expressions of B-cell lymphoma 2 (Bcl-2) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fentanyl; Ischemia-reperfusion; Myocardial apoptosis; Myocardial infarction; Hemodynamic parameters; B-cell lymphoma 2; Bax.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200603

Induction of oral tolerance and the effect of interleukin-4 on murine skin allograft rejection
Provedor de dados: 56
Autores: Dettino,A.L.A.; Duarte,A.J.S.; Sato,M.N.
We studied the effect of oral and portal vein administration of alloantigens on mouse skin allograft survival. Graft receptor BALB/c mice received spleen cells (30, 90, 150 or 375 x 10(6)) from donor C57BL/6 mice intragastrically on three successive days, starting seven days before the skin graft. Allograft survival was significantly increased with the feeding of 150 x 10(6) allogeneic spleen cells by one gavage (median survival of 12 vs 14 days, P &lt;= 0.005) or when 300 x 10(6) cells were given in six gavage (12 vs 14 days, P &lt; 0.04). A similar effect was observed when 150 x 10(6) spleen cells were injected into the portal vein (12 vs 14 days, P &lt;= 0.03). Furthermore, prolonged allograft survival was observed with subcutaneous (12 vs 16 days, P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse oral tolerance; Intraportal route; Skin transplantation; Interleukin-4; Alloantigen.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300022

Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions
Provedor de dados: 56
Autores: Seabra,A.D.; Moraes,S.A.S.; Batista,E.J.O.; Garcia,T.B.; Souza,M.C.; Oliveira,K.R.M.; Herculano,A.M.
Muscular atrophy is a progressive degeneration characterized by muscular proteolysis, loss of mass and decrease in fiber area. Tendon rupture induces muscular atrophy due to an intrinsic functional connection. Local inhibition of nitric oxide synthase (NOS) by Nω-nitro-L-arginine methyl ester (L-NAME) accelerates tendon histological recovery and induces functional improvement. Here we evaluate the effects of such local nitrergic inhibition on the pattern of soleus muscle regeneration after tenotomy. Adult male Wistar rats (240 to 280 g) were divided into four experimental groups: control (n=4), tenotomized (n=6), vehicle (n=6), and L-NAME (n=6). Muscular atrophy was induced by calcaneal tendon rupture in rats. Changes in muscle wet weight and total protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Muscle regeneration; Atrophy; Tendon; Tenotomy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300601

Risk factors for ovarian failure in patients with systemic lupus erythematosus
Provedor de dados: 56
Autores: Medeiros,M.M.C.; Silveira,V.A.L.; Menezes,A.P.T.; Carvalho,R.C.
The aim of the present study was to identify the risk factors for ovarian failure in patients with systemic lupus erythematosus. Seventy-one women aged 17 to 45 years with systemic lupus erythematosus were studied. Patients were interviewed and their medical records reviewed. Demographic characteristics, clinical and serologic profiles, and menstrual and obstetric histories were recorded. Disease activity was measured by the systemic lupus erythematosus disease activity index. Serum FSH, LH, estradiol, progesterone, TSH, prolactin, and antimicrosomal and antithyroglobulin antibodies were measured. Patients who developed ovarian failure were compared to those who did not. Ovarian failure occurred in 11 patients (15.5%) and nine had premature menopause...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovarian failure; Systemic lupus erythematosus; Risk factors; Cyclophosphamide; Premature menopause.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200008

Gastric emptying in rats with acetaminophen-induced hepatitis
Provedor de dados: 56
Autores: Hessel,G.; Collares,E.F.
The objective of this work was to study the gastric emptying (GE) of liquids in fasted and sucrose-fed rats with toxic hepatitis induced by acetaminophen. The GE of three test meals (saline, glucose and mayonnaise) was evaluated in Wistar rats. For each meal, the animals were divided into two groups (N = 24 each). Group I was fed a sucrose diet throughout the experiment (66 h) while group II was fasted. Forty-two hours after the start of the experiment, each group was divided into two subgroups (N = 12 each). Subgroup A received a placebo and subgroup B was given acetaminophen (1 g/kg). Twenty-four hours later, the GE of the three test meals was assessed and blood samples were collected to measure the serum levels of alanine aminotransferase (ALT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Acetaminophen; Experimental hepatitis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900006

Immunoglobulin production is impaired in protein-deprived mice and can be restored by dietary protein supplementation
Provedor de dados: 56
Autores: Amaral,J.F.; Foschetti,D.A.; Assis,F.A.; Menezes,J.S.; Vaz,N.M.; Faria,A.M.C.
Most contacts with food protein and microbiota antigens occur at the level of the gut mucosa. In animal models where this natural stimulation is absent, such as germ-free and antigen-free mice, the gut-associated lymphoid tissue (GALT) and systemic immunological activities are underdeveloped. We have shown that food proteins play a critical role in the full development of the immune system. C57BL/6 mice weaned to a diet in which intact proteins are replaced by equivalent amounts of amino acids (Aa diet) have a poorly developed GALT as well as low levels of serum immunoglobulins (total Ig, IgG, and IgA, but not IgM). In the present study, we evaluated whether the introduction of a protein-containing diet in 10 adult Aa-fed C57BL/6 mice could restore their...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Food proteins; Immunoglobulins; Maturation; Mucosa.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200009

Evaluation of antivenoms in the neutralization of hyperalgesia and edema induced by Bothrops jararaca and Bothrops asper snake venoms
Provedor de dados: 56
Autores: Picolo,G.; Chacur,M.; Gutiérrez,J.M.; Teixeira,C.F.P.; Cury,Y.
Neutralization of hyperalgesia induced by Bothrops jararaca and B. asper venoms was studied in rats using bothropic antivenom produced at Instituto Butantan (AVIB, 1 ml neutralizes 5 mg B. jararaca venom) and polyvalent antivenom produced at Instituto Clodomiro Picado (AVCP, 1 ml neutralizes 2.5 mg B. aspar venom). The intraplantar injection of B. jararaca and B. asper venoms caused hyperalgesia, which peaked 1 and 2 h after injection, respectively. Both venoms also induced edema with a similar time course. When neutralization assays involving the independent injection of venom and antivenom were performed, the hyperalgesia induced by B. jararaca venom was neutralized only when bothropic antivenom was administered iv 15 min before venom injection, whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca venom; Bothrops asper venom; Hyperalgesia; Edema; Antivenom; Neutralization.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000016

Effectiveness of highly active antiretroviral therapy using non-brand name drugs in Brazil
Provedor de dados: 56
Autores: May,S.B.; Barroso,P.F.; Nunes,E.P.; Barcaui,H.S.; Almeida,M.M.T.; Costa,M.D.; Faulhaber,J.C.; Santoro-Lopes,G.; Schechter,M.
In Brazil, HIV-infected individuals receive drugs (including non-brand name drugs which comprise locally produced generics and drugs that have not been tested in bioequivalence trials) free of charge from the government. The objective of the present study was to evaluate the effectiveness of highly active antiretroviral therapy (HAART) in Rio de Janeiro, Brazil, where non-brand drugs are widely used. For this purpose, we estimated the proportion of subjects with virologic failure (plasma HIV viral load greater than 400 copies/mL at 6 months after initiation of treatment). This was a retrospective cohort study of drug-naive HIV-infected subjects who initiated HAART. Subjects were included in the analysis if they were 18 years of age or older, were treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV/AIDS; Antiretroviral; Non-brand name drugs; Brazil; Virologic failure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400014

The effect of methotrexate and azathioprine on the serum levels of IgA-a1-antitrypsin complex in juvenile chronic arthritis
Provedor de dados: 56
In the present study we investigated the influence of methotrexate (MTX) and azathioprine (AZA) on the serum levels of the IgA-&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;1-antitrypsin (IgA-AT) complex in patients with the systemic form of juvenile chronic arthritis (JCA). Fifty-six JCA patients (22 treated with MTX, 18 treated with AZA, and 16 not treated with any immunosuppressive agent) were enrolled in the study. MTX dosage ranged from 0.3 to 0.5 mg kg-1 week-1, while AZA was given daily at an average dose of 1 mg/kg. MTX was given for 13 months (SD = 7 months) whereas AZA for 11 months (SD = 6 months). The average value of the complex was higher in JCA patients than in both control groups (0.74 ± 0.73 U vs 0.37 ± 0.13 U (control children), P&amp;lt;0.001...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Juvenile chronic arthritis IgA-&lt; FONT FACE=Symbol&gt; A&lt; /FONT&gt; 1-antitrypsin complex methotrexate azathioprine C-reactive protein &lt; FONT FACE=Symbol&gt; A&lt; /FONT&gt; 1-acid-glycoprotein &lt; FONT FACE=Symbol&gt; A&lt; /FONT&gt; 1-antichymotrypsin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600010

Is the Thoroughbred race-horse under chronic stress?
Provedor de dados: 56
Autores: Nogueira,G.P.; Barnabe,R.C.
Thoroughbred fillies were divided into three groups according to age: group 1, 7 fillies aged 1 to 2 years (G1) starting the training program; group 2, 9 fillies aged 2 to 3 years (G2) in a full training program; group 3, 8 older fillies 3 to 4 years of age (G3) training and racing. Blood samples were collected weekly from July to December. Cortisol was quantified using a solid phase DPC kit. The intra- and interassay coefficients of variation were 12.5% and 15.65% and sensitivity was 1.9 ± 0.2 nmol/l. The semester average of cortisol levels varied between groups: G1 = 148.8 ± 6.7, G2 = 125.7 ± 5.8, G3 = 101.1 ± 5.4 nmol/l, with G3 differing statistically from the other groups. The lower cortisol levels observed in the older fillies lead us to propose that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horses; Cortisol; Exercise; Chronic stress.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000016

Participation of nitric oxide in the nucleus isthmi in CO2-drive to breathing in toads
Provedor de dados: 56
Autores: Gargaglioni,L.H.; Branco,L.G.S.
The nucleus isthmi (NI) is a mesencephalic structure of the amphibian brain. It has been reported that NI plays an important role in integration of CO2 chemoreceptor information and glutamate is probably involved in this function. However, very little is known about the mechanisms involved. Recently, it has been shown that nitric oxide synthase (NOS) is expressed in the brain of the frog. Thus the gas nitric oxide (NO) may be involved in different functions in the brain of amphibians and may act as a neurotransmitter or neuromodulator. We tested the hypothesis that NO plays a role in CO2-drive to breathing, specifically in the NI comparing pulmonary ventilation, breathing frequency and tidal volume, after microinjecting 100 nmol/0.5 µl of L-NAME (a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Amphibian; Hypercapnia; Ventilation; Nucleus isthmi; Toad; CO2; L-NAME.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100010

Sequence change in the HS2-LCR and Gg-globin gene promoter region of sickle cell anemia patients
Provedor de dados: 56
Autores: Adorno,E.V.; Moura-Neto,J.P.; Lyra,I.; Zanette,A.; Santos,L.F.O.; Seixas,M.O.; Reis,M.G.; Goncalves,M.S.
The fetal hemoglobin (HbF) levels and ßS-globin gene haplotypes of 125 sickle cell anemia patients from Brazil were investigated. We sequenced the Gg- and Ag-globin gene promoters and the DNase I-2 hypersensitive sites in the locus control regions (HS2-LCR) of patients with HbF level disparities as compared to their ßS haplotypes. Sixty-four (51.2%) patients had CAR/Ben genotype; 36 (28.8%) Ben/Ben; 18 (14.4%) CAR/CAR; 2 (1.6%) CAR/Atypical; 2 (1.6%) Ben/Cam; 1 (0.8%) CAR/Cam; 1 (0.8%) CAR/Arab-Indian, and 1 (0.8%) Sen/Atypical. The HS2-LCR sequence analyses demonstrated a c.-10.677G&gt;A change in patients with the Ben haplotype and high HbF levels. The Gg gene promoter sequence analyses showed a c.-157T&gt;C substitution shared by all patients, and a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fetal hemoglobin; Sickle cell anemia; SSS-globin gene haplotypes; Locus control region; G-globin promoter.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200003

Humoral and cellular immune responses to Blomia tropicalis and concanavalin A-binding fractions in atopic patients
Provedor de dados: 56
Autores: Alves,R.; Silva,D.A.O.; Fernandes,J.F.C.; Almeida,K.C.; Ynoue,L.H.; Bernardes,C.T.V.; Moreira,P.F.S.; Gennari-Cardoso,M.L.; Sung,S.J.; Taketomi,E.A.
Blomia tropicalis, Dermatophagoides pteronyssinus and D. farinae are prevalent house dust mites. Concanavalin A-binding components derived from B. tropicalis (Bt-ConA extract) are highly immunogenic in allergic diseases. The aim of the present study was to evaluate the humoral and cellular immune responses to B. tropicalis in mite-sensitized patients. A total of 137 patients with allergic rhinitis with/without asthma and 109 non-atopic subjects were selected and analyzed by the skin prick test, and for total serum IgE and specific IgE levels to both Bt-total and Bt-ConA extracts, their proliferative response and cytokine (IFN-γ and IL-5) production by peripheral blood mononuclear cells (PBMC) stimulated with both extracts. Skin prick test showed that 70%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blomia tropicalis; Concanavalin A; Cellular proliferation; IFN-γ; IL-5; Immunoglobulin E.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900006

RNAi-mediated knockdown of FANCF suppresses cell proliferation, migration, invasion, and drug resistance potential of breast cancer cells
Provedor de dados: 56
Autores: Zhao,L.; Li,N.; Yu,J.K.; Tang,H.T.; Li,Y.L.; He,M.; Yu,Z.J.; Bai,X.F.; Zheng,Z.H.; Wang,E.H.; Wei,M.J.
Fanconi anemia complementation group F protein (FANCF) is a key factor, which maintains the function of FA/BRCA, a DNA damage response pathway. However, the functional role of FANCF in breast cancer has not been elucidated. We performed a specific FANCF-shRNA knockdown of endogenous FANCF in vitro. Cell viability was measured with a CCK-8 assay. DNA damage was assessed with an alkaline comet assay. Apoptosis, cell cycle, and drug accumulation were measured by flow cytometry. The expression levels of protein were determined by Western blot using specific antibodies. Based on these results, we used cell migration and invasion assays to demonstrate a crucial role for FANCF in those processes. FANCF shRNA effectively inhibited expression of FANCF. We found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anemia complementation group F protein; Breast neoplasms; Tumor cell line.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100024

Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system
Provedor de dados: 56
Autores: Langella,P.; Le Loir,Y.
Lactic acid bacteria (LAB) are Gram-positive bacteria and are generally regarded as safe (GRAS) organisms. Therefore, LAB could be used for heterologous protein secretion and they are good potential candidates as antigen delivery vehicles. To develop such live vaccines, a better control of protein secretion is required. We developed an efficient secretion system in the model LAB, Lactococcus lactis. Staphylococcal nuclease (Nuc) was used as the reporter protein. We first observed that the quantity of secreted Nuc correlated with the copy number of the cloning vector. The nuc gene was cloned on a high-copy number cloning vector and no perturbation of the metabolism of the secreting strain was observed. Replacement of nuc native promoter by a strong...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein secretion; Staphylococcal nuclease; Lactococcus lactis; Propeptide; Bovine coronavirus; Epitope.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200007

Chronic obstructive pulmonary disease is underdiagnosed and undertreated in São Paulo (Brazil): results of the PLATINO study
Provedor de dados: 56
Autores: Nascimento,O.A.; Camelier,A.; Rosa,F.W.; Menezes,A.M.B.; Pérez-Padilla,R.; Jardim,J.R.
Chronic obstructive pulmonary disease (COPD) is a common disease in adults over 40 years of age and has a great social and economic impact. It remains little recognized and undertreated even in developed countries. However, there are no data about its diagnosis and treatment in Brazil. The objectives of the present study were to evaluate the proportion of COPD patients who had never been diagnosed and to determine if the COPD patients who had been identified were receiving appropriate treatment. The Latin American Project for the Investigation of Obstructive Lung Disease (PLATINO) was a randomized epidemiological study of adults over 40 years living in five metropolitan areas, including São Paulo. The studied sample was randomly selected from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Diagnosis; Treatment; Vaccination; Smoking; Latin American Project for the Investigation of Obstructive Lung Disease.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700001

Protein tyrosine kinase inhibitors modify kainic acid-induced epileptiform activity and mossy fiber sprouting but do not protect against limbic cell death
Provedor de dados: 56
Autores: Queiroz,C.M.; Mello,L.E.
Intrahippocampal administration of kainic acid (KA) induces synaptic release of neurotrophins, mainly brain-derived neurotrophic factor, which contributes to the acute neuronal excitation produced by the toxin. Two protein tyrosine kinase inhibitors, herbimycin A and K252a, were administered intracerebroventricularly, in a single dose, to attenuate neurotrophin signaling during the acute effects of KA, and their role in epileptogenesis was evaluated in adult, male Wistar rats weighing 250-300 g. The latency for the first Racine stage V seizure was 90 ± 8 min in saline controls (N = 4) which increased to 369 ± 71 and 322 ± 63 min in animals receiving herbimycin A (1.74 nmol, N = 4) and K252a (10 pmol, N = 4), respectively. Behavioral alterations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbimycin A; K252a; Cell death; EEG; Epilepsy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500009

High pressure-sensitive gene expression in Lactobacillus sanfranciscensis
Provedor de dados: 56
Autores: Vogel,R.F.; Pavlovic,M.; Hörmann,S.; Ehrmann,M.A.
Lactobacillus sanfranciscensis is a Gram-positive lactic acid bacterium used in food biotechnology. It is necessary to investigate many aspects of a model organism to elucidate mechanisms of stress response, to facilitate preparation, application and performance in food fermentation, to understand mechanisms of inactivation, and to identify novel tools for high pressure biotechnology. To investigate the mechanisms of the complex bacterial response to high pressure we have analyzed changes in the proteome and transcriptome by 2-D electrophoresis, and by microarrays and real time PCR, respectively. More than 16 proteins were found to be differentially expressed upon high pressure stress and were compared to those sensitive to other stresses. Except for one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High pressure; Lactobacillus sanfranciscensis; Proteome; Transcriptome; Stress response.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800013

Oxidative stress and cellular immunity in patients with recurrent aphthous ulcers
Provedor de dados: 56
Autores: Avci,E.; Akarslan,Z.Z.; Erten,H.; Coskun-Cevher,S.
Recurrent aphthous ulcer (RAU) is an inflammatory condition of the oral mucosa characterized by painful, well-circumscribed, single or multiple round or ovoid ulcerations. The exact etiologic factor(s) of these ulcerations are not yet understood. The objective of this study was to evaluate inflammatory processes and free radical metabolism of 25 patients with RAUs compared to 25 healthy controls. The levels of malondialdehyde (MDA) and glutathione (GSH) were determined by high-performance liquid chromatography. Tumor necrosis factor-alpha (TNF-α), interleukin-2 (IL-2), IL-10, and IL-12 were determined by ELISA. Nitric oxide (NO), myeloperoxidase (MPO), total antioxidant status (TAS), and total oxidant status (TOS) levels were measured spectroscopically in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recurrent aphthous ulcer; Oxidative stress; Cytokines.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500355

Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
Provedor de dados: 56
Autores: Teixeira,R.C.; Baêta,B.A.; Ferreira,J.S.; Medeiros,R.C.; Maya-Monteiro,C.M.; Lara,F.A.; Bell-Sakyi,L.; Fonseca,A.H.
This study aimed to describe the association of Borrelia burgdorferi s.s. with ixodid tick cell lines by flow cytometry and fluorescence and confocal microscopy. Spirochetes were stained with a fluorescent membrane marker (PKH67 or PKH26), inoculated into 8 different tick cell lines and incubated at 30°C for 24 h. PKH efficiently stained B. burgdorferi without affecting bacterial viability or motility. Among the tick cell lines tested, the Rhipicephalus appendiculatus cell line RA243 achieved the highest percentage of association/internalization, with both high (90%) and low (10%) concentrations of BSK-H medium in tick cell culture medium. Treatment with cytochalasin D dramatically reduced the average percentage of cells with internalized spirochetes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borrelia burgdorferi; Tick cell lines; Phagocytosis; Fluorescent membrane marker.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700601

Autoantibody production in chronic idiopathic urticaria is not associated with Helicobacter pylori infection
Provedor de dados: 56
Autores: Atta,A.M.; Rodrigues,M.Z.A.; Sousa,C.P.; Medeiros Júnior,M.; Sousa-Atta,M.L.B.
Chronic idiopathic urticaria (CIU) is a dermatological syndrome, characterized by raised erythematous skin lesions, that affects 20% of the general population and has been associated with autoimmunity. However, some reports have also suggested a close relationship between CIU and Helicobacter pylori infection, which is endemic in developing countries and associated with chronic gastritis, peptic ulcer disease, and gastric carcinoma. In the present study, we investigated the occurrence of autoantibodies in sera from 23 CIU subjects infected with H. pylori and from 23 CIU subjects without this infection. The presence of anti-thyroid antibodies was determined by indirect hemagglutination assay and the presence of autoantibodies to IgE and C1INH was determined...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chronic idiopathic urticaria; Autoimmunity; Autoantibodies; Helicobacter pylori.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100002

Low serum concentrations of 25-hydroxyvitamin D in children and adolescents with systemic lupus erythematosus
Provedor de dados: 56
Autores: Peracchi,O.A.B.; Terreri,M.T.R.A.; Munekata,R.V.; Len,C.A.; Sarni,R.O.S.; Lazaretti-Castro,M.; Hilário,M.O.E.
We evaluated the concentrations of 25-hydroxyvitamin D [25(OH)D] in children and adolescents with juvenile systemic lupus erythematosus (JSLE) and associated them with disease duration and activity, use of medication (chloroquine and glucocorticoids), vitamin D intake, calcium and alkaline phosphatase levels, and bone mineral density. Thirty patients with JSLE were evaluated and compared to 30 healthy individuals, who were age and gender matched. Assessment was performed of clinical status, disease activity, anthropometry, laboratory markers, and bone mineral density. The 30 patients included 25 (83.3%) females and 16 (53.3%) Caucasians, with a mean age of 13.7 years. The mean age at diagnosis was 10.5 years and mean disease duration was 3.4 years. Mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile systemic lupus erythematosus; Vitamin D; Calcium; Bone metabolism; Parathormone.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800721

Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis
Provedor de dados: 56
Autores: Mendonça,D.M.F.; Chimelli,L.; Martinez,A.M.B.
Amyotrophic lateral sclerosis (ALS), a neurodegenerative disease of unknown etiology, affects motor neurons leading to atrophy of skeletal muscles, paralysis and death. There is evidence for the accumulation of neurofilaments (NF) in motor neurons of the spinal cord in ALS cases. NF are major structural elements of the neuronal cytoskeleton. They play an important role in cell architecture and differentiation and in the determination and maintenance of fiber caliber. They are composed of three different polypeptides: light (NF-L), medium (NF-M) and heavy (NF-H) subunits. In the present study, we performed a morphological and quantitative immunohistochemical analysis to evaluate the accumulation of NF and the presence of each subunit in control and ALS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyotrophic lateral sclerosis; Spheroids; Neurofilament proteins; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600015

An improved experimental model for peripheral neuropathy in rats
Provedor de dados: 56
Autores: Dias,Q.M.; Rossaneis,A.C.; Fais,R.S.; Prado,W.A.
A modification of the Bennett and Xie chronic constriction injury model of peripheral painful neuropathy was developed in rats. Under tribromoethanol anesthesia, a single ligature with 100% cotton glace thread was placed around the right sciatic nerve proximal to its trifurcation. The change in the hind paw reflex threshold after mechanical stimulation observed with this modified model was compared to the change in threshold observed in rats subjected to the Bennett and Xie or the Kim and Chung spinal ligation models. The mechanical threshold was measured with an automated electronic von Frey apparatus 0, 2, 7, and 14 days after surgery, and this threshold was compared to that measured in sham rats. All injury models produced significant hyperalgesia in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic constriction injury; Chronic pain model; Neuropathy; Peripheral nerve lesion; Spinal nerve ligation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300253

Influence of expiratory positive airway pressure on cardiac autonomic modulation at rest and in submaximal exercise in COPD patients
Provedor de dados: 56
Autores: da L. Goulart,C.; San Martin,E.A.; Mansour,K.M.K.; Schneiders,P.B.; da Silva,A.L.G.
The aim of this study was to evaluate the effect of expiratory positive airway pressure (EPAP) on heart rate variability (HRV) indices at rest and during 6-min walk test (6MWT) in chronic obstructive pulmonary disease (COPD) patients. Fifteen moderate to severe COPD patients were randomized and evaluated with and without (Non-EPAP) a 5 cmH2O EPAP device. Respiratory rate (RR) was collected at rest (5 min), during the 6MWT (5 min), and at recovery (5 min). Indices of HRV were computed in the time domain, in the frequency domain, and nonlinear analysis. For EPAP and Non-EPAP during the 6MWT, we found an increased mean heart rate (HR) (P=0.001; P=0.001) while mean RR (P=0.001; P=0.015) and RR tri index decreased (P=0.006; P=0.028). Peripheral oxygen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic nervous system; HRV; COPD; Positive-pressure respiration; Exercise test.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600605

Aspirin plus calcium supplementation to prevent superimposed preeclampsia: a randomized trial
Provedor de dados: 56
Autores: Souza,E.V.; Torloni,M.R.; Atallah,A.N.; dos Santos,G.M.S.; Kulay Jr,L.; Sass,N.
Preeclampsia is an important cause of maternal and perinatal morbidity and mortality. Previous studies have tested calcium supplementation and aspirin separately to reduce the incidence of preeclampsia but not the effects of combined supplementation. The objective of this study was to investigate the effectiveness of aspirin combined with calcium supplementation to prevent preeclampsia in women with chronic hypertension. A double-blind, placebo-controlled randomized clinical trial was carried out at the antenatal clinic of a large university hospital in São Paulo, SP, Brazil. A total of 49 women with chronic hypertension and abnormal uterine artery Doppler at 20-27 weeks gestation were randomly assigned to receive placebo (N = 26) or 100 mg aspirin plus 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Hypertension; Ultrasonography; Doppler; Calcium carbonate; Aspirin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500419

Induction of Fos protein immunoreactivity by spinal cord contusion
Provedor de dados: 56
Autores: Del-Bel,E.A.; Borges,C.A.G.; Defino,H.L.A.; Guimarães,F.S.
The objective of the present study was to identify neurons in the central nervous system that respond to spinal contusion injury in the rat by monitoring the expression of the nuclear protein encoded by the c-fos gene, an activity-dependent gene, in spinal cord and brainstem regions. Rats were anesthetized with urethane and the injury was produced by dropping a 5-g weight from 20.0 cm onto the exposed dura at the T10-L1 vertebral level (contusion group). The spinal cord was exposed but not lesioned in anesthetized control animals (laminectomy group); intact animals were also subjected to anesthesia (intact control). Behavioral alterations were analyzed by Tarlov/Bohlman scores, 2 h after the procedures and the animals were then perfused for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fos immunohistochemistry; Intermediolateral column neurons; Reticular formation; Area postrema; Dorsal horn; Solitary tract nuclei; Laminectomy; Spinal cord injury.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500006

Interactions of laminin with the amyloid ß peptide: Implications for Alzheimer's disease
Provedor de dados: 56
Autores: Morgan,C.; Inestrosa,N.C.
Extensive neuronal cell loss is observed in Alzheimer's disease. Laminin immunoreactivity colocalizes with senile plaques, the characteristic extracellular histopathological lesions of Alzheimer brain, which consist of the amyloid ß (Aß) peptide polymerized into amyloid fibrils. These lesions have neurotoxic effects and have been proposed to be a main cause of neurodegeneration. In order to understand the pathological significance of the interaction between laminin and amyloid, we investigated the effect of laminin on amyloid structure and toxicity. We found that laminin interacts with the Aß1-40 peptide, blocking fibril formation and even inducing depolymerization of preformed fibrils. Protofilaments known to be intermediate species of Aß fibril formation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Laminin; Amyloid ß peptide; Neuronal injury; Neurodegenerative disease.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500006

Early detection of metabolic and energy disorders by thermal time series stochastic complexity analysis
Provedor de dados: 56
Autores: Lutaif,N.A.; Palazzo Jr,R.; Gontijo,J.A.R.
Maintenance of thermal homeostasis in rats fed a high-fat diet (HFD) is associated with changes in their thermal balance. The thermodynamic relationship between heat dissipation and energy storage is altered by the ingestion of high-energy diet content. Observation of thermal registers of core temperature behavior, in humans and rodents, permits identification of some characteristics of time series, such as autoreference and stationarity that fit adequately to a stochastic analysis. To identify this change, we used, for the first time, a stochastic autoregressive model, the concepts of which match those associated with physiological systems involved and applied in male HFD rats compared with their appropriate standard food intake age-matched male controls...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Time series temperature; Thermal homeostasis; High-fat diet; Metabolic disorder predictor; Autoregressive models.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100070

Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase
Provedor de dados: 56
Autores: Li,Y.; Chen,L.J.; Jiang,F.; Yang,Y.; Wang,X.X.; Zhang,Z.; Li,Z.; Li,L.
Hormesis is an adaptive response to a variety of oxidative stresses that renders cells resistant to harmful doses of stressing agents. Caffeic acid (CaA) is an important antioxidant that has protective effects against DNA damage caused by reactive oxygen species (ROS). However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved. We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H2O2)-mediated decreases in cell viability, and decreased the extent of H2O2-induced DNA double-strand breaks (DSBs). In L-02 cells exposed to H2O2, CaA treatment reduced ROS levels, which might have played a protective role. CaA also activated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caffeic acid; Hormesis; Antioxidants; DNA double-strand breaks; Extracellular signal-regulated kinase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600502

Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe2O3 nanoparticles
Provedor de dados: 56
Autores: Yan,S.Y.; Chen,M.M.; Fan,J.G.; Wang,Y.Q.; Du,Y.Q.; Hu,Y.; Xu,L.M.
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe2O3 nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe2O3 nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe2O3 MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe2O3 MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fe2O3; Magnetic nanoparticles; Magnetic fluid hyperthermia; Hepatocarcinoma cells; Liver cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100947

Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component
Provedor de dados: 56
Autores: Pires,R.R.; Oshiro,T.M.; Itami,D.M.; Fernandes,I.; Macedo-Soares,M.F.
Ascaris suum allergenic components (PIII) separated by gel filtration chromatography of an adult worm extract were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells using polyethylene glycol (MW 1450) as fusogen. The hybridomas were cultured in HAT-containing medium and cloned at limiting dilutions. Supernatants from the growing hybrids were screened by ELISA using plates coated with PIII or the A. suum crude extract. The monoclonal antibody obtained, named MAC-3 (mouse anti-A. suum allergenic component), is an IgG1 kappa mouse immunoglobulin that specifically recognizes a 29,000 molecular weight protein (called allergenic protein) with an affinity constant of 1.7 x 10(9) M-1. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ascaris suum; Allergen; IgE; Monoclonal antibodies.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800009

Low ethanol consumption increases insulin sensitivity in Wistar rats
Provedor de dados: 56
Autores: Furuya,D.T.; Binsack,R.; Machado,U.F.
Several human studies suggest that light-to-moderate alcohol consumption is associated with enhanced insulin sensitivity, but these studies are not free of conflicting results. To determine if ethanol-enhanced insulin sensitivity could be demonstrated in an animal model, male Wistar rats were fed a standard chow diet and received drinking water without (control) or with different ethanol concentrations (0.5, 1.5, 3, 4.5 and 7%, v/v) for 4 weeks ad libitum. Then, an intravenous insulin tolerance test (IVITT) was performed to determine insulin sensitivity. Among the ethanol groups, only the 3% ethanol group showed an increase in insulin sensitivity based on the increase of the plasma glucose disappearance rate in the IVITT (30%, P&lt;0.05). In addition, an...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Insulin sensitivity; Ethanol; Liver glycogen; Low ethanol consumption.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100017

T-cell lymphoma with rhabdomyolysis: case report and literature review
Provedor de dados: 56
Autores: Ouyang,Zhao-Zhong; Peng,Ting-Sheng; Cao,Qing-Hua; Ouyang,Yin; Li,Jun-Xun; Wei,Qiu-Xiang; Zhan,Hong.
Rhabdomyolysis refers to the destruction or disintegration of striated muscles. This syndrome is characterized by muscle breakdown and necrosis, resulting in the leakage of intracellular muscle constituents into the circulation and extracellular fluid. We report a rare case of rhabdomyolysis complicating multi-organ failure caused by T-cell lymphoma in a 32-year-old woman. The final diagnosis was rhabdomyolysis caused by peripheral T-cell lymphoma based on bone marrow aspirate and biopsy.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Rhabdomyolysis; Multiple organ failure; T-cell lymphoma; Hemophagocytic lymphohistiocytosis; Case report.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100501

Relationship of cyclosporin and sirolimus blood concentrations regarding the incidence and severity of hyperlipidemia after kidney transplantation
Provedor de dados: 56
Autores: Spinelli,G.A.; Felipe,C.R.; Machado,P.G.; Garcia,R.; Casarini,D.E.; Moreira,S.R.; Park,S.I.; Tedesco-Silva Jr.,H.; Medina-Pestana,J.O.
The influence of drug concentrations on the development of persistent posttransplant hyperlipidemia was investigated in 82 patients who received cyclosporin A (CsA) and prednisone plus sirolimus (SRL) (52) or azathioprine (AZA) (30) during the first year after transplantation. Blood levels of CsA and SRL, daily doses of AZA and prednisone, and cholesterol, triglyceride, and glucose concentrations were determined during each visit (pretransplant and 30, 60, 90, 120, 180, and 360 days posttransplant). Persistent hyperlipidemia was defined as one-year average steady-state cholesterol (CavCHOL) or triglyceride (CavTG) concentrations above 240 and 200 mg/dL, respectively. Mean cholesterol and triglyceride concentrations increased after transplantation (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperlipidemia; Cholesterol; Triglyceride; Kidney transplantation; Cyclosporin A; Sirolimus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100003

Epidermal growth factor receptor (EGFR) mutations in lung cancer: preclinical and clinical data
Provedor de dados: 56
Autores: Jorge,S.E.D.C.; Kobayashi,S.S.; Costa,D.B.
Lung cancer leads cancer-related mortality worldwide. Non-small-cell lung cancer (NSCLC), the most prevalent subtype of this recalcitrant cancer, is usually diagnosed at advanced stages, and available systemic therapies are mostly palliative. The probing of the NSCLC kinome has identified numerous nonoverlapping driver genomic events, including epidermal growth factor receptor (EGFR) gene mutations. This review provides a synopsis of preclinical and clinical data on EGFR mutated NSCLC and EGFR tyrosine kinase inhibitors (TKIs). Classic somatic EGFR kinase domain mutations (such as L858R and exon 19 deletions) make tumors addicted to their signaling cascades and generate a therapeutic window for the use of ATP-mimetic EGFR TKIs. The latter inhibit these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Lung cancer; Non-small-cell lung cancer; EGFR; EGFR inhibitor; Precision therapies; Resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100929

The MTR A2756G polymorphism is associated with an increase of plasma homocysteine concentration in Brazilian individuals with Down syndrome
Provedor de dados: 56
Autores: Biselli,J.M; Goloni-Bertollo,E.M; Haddad,R; Eberlin,M.N; Pavarino-Bertelli,E.C.
Individuals with Down syndrome (DS) present decreased homocysteine (Hcy) concentration, reflecting a functional folate deficiency secondary to overexpression of the cystathionine ß-synthase gene. Since plasma Hcy may be influenced by genetic polymorphisms, we evaluated the influence of C677T and A1298C polymorphisms in the methylenetetrahydrofolate reductase gene (MTHFR), of A2756G polymorphism in the methionine synthase gene (MTR), and of A80G polymorphism in the reduced folate carrier 1 gene on Hcy concentrations in Brazilian DS patients. Fifty-six individuals with free trisomy 21 were included in the study. Plasma Hcy concentrations were measured by liquid chromatography_tandem mass spectrometry with linear regression coefficient r² = 0.9996, average...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Down syndrome; Trisomy 21; Polymorphism; Homocysteine; Mass spectrometry (LC-MS/MS).
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100006

Eosinophil-active chemokines: assessment of in vivo activity
Provedor de dados: 56
Autores: Teixeira,M.M.
The selective recruitment of eosinophils in tissue is a striking feature of allergic diseases. Recently, a family of chemoattractant molecules, namely chemokines, has been described which potently activates eosinophil function in vitro. We have developed a murine model of eosinophil recruitment to compare the relative potency and efficacy of chemokines in vivo. Of the chemokines tested, only eotaxin and MIP-1&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; induced significant accumulation of eosinophils in vivo, but eotaxin was more effective than MIP-1&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;. Chemokines, especially eotaxin acting via the CCR-3 receptor, may have a fundamental role in determining selective eosinophil recruitment in vivo
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eosinophils chemokines eotaxin MIP-1&lt; FONT FACE=Symbol&gt; A&lt; /font&gt; Chemokine receptors.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100003

Cardiovascular and respiratory responses to microinjection of L-glutamate into the caudal pressor area in conscious and anesthetized rats
Provedor de dados: 56
Autores: Silva,N.F.; Pires,J.G.P.; Campos,R.R.; Futuro Neto,H.A.
The role of the caudal pressor area (CPA) in the maintenance of vasomotor tonus in anesthetized and decerebrate animals has been clearly established. In conscious animals, however, the participation of CPA in the cardiovascular control remains to be fully elucidated. In the present study, unilateral L-glutamate (L-Glu) (10 and/or 20 nmol/70 nl) microinjection into CPA, in conscious male Wistar rats (250-280 g) caused a significant increase in mean arterial blood pressure (MAP; control: 112 ± 1.9 mmHg; after 20 nmol L-Glu: 139 ± 4.5 mmHg, N = 12, P&lt;0.05) and respiratory rate (control: 81 ± 3.5 breaths/min; after 10 nmol L-Glu: 92 ± 3 breaths/min, P&lt;0.05; after 20 nmol L-Glu: 104 ± 5 breaths/min, N = 6, P&lt;0.05). The subsequent anesthesia with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Caudal pressor area; Ventrolateral medulla; Blood pressure; L-glutamate; Respiration.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200013

Hepatic changes in mice chronically infected with Helicobacter trogontum
Provedor de dados: 56
Autores: Moura,S.B.; Queiroz,D.M.M.; Rocha,G.A.; Comunian,L.B.; Cara,D.C.
We infected NIH germ-free female mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the lesions it induced in the liver of this host. Fifteen mice were challenged with a single dose of H. trogontum (test group) and killed 6, 12 and 18 months after inoculation (5 animals/group). Nine animals were challenged with 0.85% saline alone (control group) and killed at the same times. Fragments from the liver, cecum and colon were obtained for microbiologic and histologic examination. Stool samples were also collected. H. trogontum was detected in the cecum, colon and/or stool samples of all test mice. As expected, the bacterium was not isolated from any specimen obtained from the control animals. On the other...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Helicobacter; Helicobacter trogontum; Animal model; Liver; Mice.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900010

Age-related changes in radiation-induced micronuclei among healthy adults
Provedor de dados: 56
Autores: Joksic,G.; Petrovic,S.; Ilic,Z.
The aim of the present study was to establish the extent of in vitro radioresponse of lymphocytes among 62 healthy adults of both genders and to estimate the distribution of baseline micronuclei and radiosensitivity among individuals of the study population using the cytochalasin block micronucleus test. A younger study group consisted of 10 males (mean age, 22.4 years; range, 21-27) and 12 females (mean age, 24.8 years; range, 20-29), whereas an older study group consisted of 18 males (mean age, 35.1 years; range, 30-44) and 22 females (mean age, 38.5 years; range, 30-48). For evaluation of radiosensitivity blood samples were irradiated in vitro using 60Co g-ray source. The radiation dose employed was 2 Gy, the dose rate 0.45 Gy/min. The study revealed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human lymphocytes; Baseline micronuclei; Radiosensitivity; Ageing.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800002

Effects of sodium valproate and carbamazepine on food competition aggression in pigeons
Provedor de dados: 56
Autores: Fachinelli,C.; Ahumada,M.; Fachinellizz,J.M.; Torrecilla,M.; Rodríguez-Echandía,E.L.
Valproate and carbamazepine (CAR) have been proposed as adjunct alternatives for the control of aggression in psychiatric patients, although no definite conclusions have been reached. We examined the effects of these drugs on food competition offensive aggression and other behaviors in high- and low-aggression food-restricted pigeons. These were divided into pairs containing previously ranked high-aggression (N = 10 pairs) and low-aggression females (N = 10 pairs). In Experiment 1, a pigeon in each pair of high- and low-aggression subjects was treated daily with an oral dose of sodium valproate (50 mg kg-1 mL saline-1) for 15 days. The other animal received the vehicle. On days 1, 7, and 15, food competition trials (10 min) were performed 60 min after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Valproate; Carbamazepine; Aggressive behavior; Food competition; Pigeons.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600015

Expression of β-catenin and c-myc during human common bile duct development: a possible role in the morphogenesis of the common bile duct
Provedor de dados: 56
Autores: Guo,W.L.; Zhang,Q.; Wang,J.
β-catenin and c-myc play important roles in the development of tissues and organs. However, little is known about their expression patterns during the development of the human common bile duct. Immunohistochemistry was used to detect β-catenin and c-myc expression in common bile duct samples from postmortem tissues of 14 premature infants and 6 spontaneously aborted fetuses. The expression of β-catenin and c-myc was also analyzed by Western blot. The samples were divided into four groups based on the stage of human fetal development: 12, 13-27, 28-37, and &gt;37 weeks. The Image-Pro Plus v. 6.0 image analysis software was used to calculate the mean qualifying score (MQS). At fetal stages 12, 13-27, 28-37, and &gt;37 weeks, MQS of β-catenin were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-catenin; C-myc; Common bile duct; Fetal development.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700594

Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
Provedor de dados: 56
Autores: Silva,N.P.; Christofolini,D.M.; Mortara,R.A.; Andrade,L.E.C.
Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK2 cells undergoing micronucleation. Nocodazol-induced micronucleated cells were examined by double indirect immunofluorescence with antibodies against coilin, fibrillarin, NOR-90/hUBF, RNA polymerase I, PM/Scl, and To/Th. Cells were imaged on a BioRad 1024-UV confocal system attached to a Zeiss Axiovert 100 microscope. Since PtK2 cells possess only one nucleolus organizer region, micronucleated cells presented only one or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cajal body; Coiled body; Micronuclei; Cell nucleolus; Nucleolus organizer region; RNA polymerase I; Nucleolar proteins.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700008

Effect of neurotrophic factor, MDP, on rats’ nerve regeneration
Provedor de dados: 56
Autores: Fornazari,A.A.; de Rezende,M.R.; Mattar Jr.,R.; Taira,R.I.; dos Santos,G.B.; Paulos,R.G.
Our objective was to determine the immune-modulating effects of the neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) on median nerve regeneration in rats. We used male Wistar rats (120-140 days of age, weighing 250-332 g) and compared the results of three different techniques of nerve repair: 1) epineural neurorrhaphy using sutures alone (group S - 10 rats), 2) epineural neurorrhaphy using sutures plus fibrin tissue adhesive (FTA; group SF - 20 rats), and 3) sutures plus FTA, with MDP added to the FTA (group SFM - 20 rats). Functional assessments using the grasp test were performed weekly for 12 weeks to identify recovery of flexor muscle function in the fingers secondary to median nerve regeneration. Histological analysis was also...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Grasping test; Neurotrophic factor; N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP).
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400009

Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells
Provedor de dados: 56
Autores: Yang,R.; Lin,Q.; Gao,H.B.; Zhang,P.
In the current literature, there is evidence that psychological factors can affect the incidence and progression of some cancers. Interleukin 6 (IL-6) is known to be elevated in individuals experiencing chronic stress and is also involved in oncogenesis and cancer progression. However, the precise mechanism of IL-6 induction by the stress-related hormone norepinephrine (NE) is not clear, and, furthermore, there are no reports about the effect of NE on IL-6 expression in gastric epithelial cells. In this study, we examined the effect of NE on IL-6 expression in immortalized human gastric epithelial cells (GES-1 cells). Using real-time PCR and enzyme-linked immunoassay, we demonstrated that NE can induce IL-6 mRNA and protein expression in GES-1 cells. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Norepinephrine; GES-1 cells; Interleukin-6.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200101

Calciuria and preeclampsia
Provedor de dados: 56
Urinary calcium excretion has been reported to be diminished in preeclampsia. The objective of the present study was to determine urinary calcium excretion in pregnant patients with chronic arterial hypertension (CAH) and preeclampsia (PE), and in normotensive patients (N). Forty-four pregnant patients (gestional age, 20-42 weeks; 18 CAH, 17 PE, 9 N) were evaluated for calciuria, proteinuria, plasma uric acid and blood pressure. Patients with PE (82 ± 15.1 mg/24 h) showed significantly lower calciuria (P&amp;lt;0.05) than the group with CAH (147 ± 24.9 mg/24 h) and the N group (317 ± 86.0 mg/24 h) (P&amp;lt;0.05, Student t-test). Plasma uric acid was significantly higher in the PE group (6.1 ± 0.38 mg/dl) than the CAH group (5.0 ± 0.33 mg/dl;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Hypertension; Calciuria; Kidney function; High-risk pregnancy.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400007

Some physico-chemical parameters that influence proteinase K resistance and the infectivity of PrP Sc after high pressure treatment
Provedor de dados: 56
Autores: Heindl,P.; Fernández García,A.; Büttner,M.; Voigt,H.; Butz,P.; Tauscher,B.; Pfaff,E.
Crude brain homogenates of terminally diseased hamsters infected with the 263 K strain of scrapie (PrP Sc) were heated and/or pressurized at 800 MPa at 60ºC for different times (a few seconds or 5, 30, 120 min) in phosphate-buffered saline (PBS) of different pH and concentration. Prion proteins were analyzed on immunoblots for their proteinase K (PK) resistance, and in hamster bioassays for their infectivity. Samples pressurized under initially neutral conditions and containing native PrP Sc were negative on immunoblots after PK treatment, and a 6-7 log reduction of infectious units per gram was found when the samples were pressurized in PBS of pH 7.4 for 2 h. A pressure-induced change in the protein conformation of native PrP Sc may lead to less PK...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Native prion protein; Pressure; Inactivation; PH; Proteinase K sensitivity; Prion conformation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800010

Possible roles of COX-1 in learning and memory impairment induced by traumatic brain injury in mice
Provedor de dados: 56
Autores: Shang,J.L.; Cheng,Q.; Yang,W.F.; Zhang,M.; Cui,Y.; Wang,Y.F.
People who suffer from traumatic brain injury (TBI) often experience cognitive deficits in spatial reference and working memory. The possible roles of cyclooxygenase-1 (COX-1) in learning and memory impairment in mice with TBI are far from well known. Adult mice subjected to TBI were treated with the COX-1 selective inhibitor SC560. Performance in the open field and on the beam walk was then used to assess motor and behavioral function 1, 3, 7, 14, and 21 days following injury. Acquisition of spatial learning and memory retention was assessed using the Morris water maze on day 15 post-TBI. The expressions of COX-1, prostaglandin E2 (PGE2), interleukin (IL)-6, brain-derived neurotrophic factor (BDNF), platelet-derived growth factor BB (PDGF-BB), synapsin-I,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Traumatic brain injury; COX-1; Learning and memory; SC560.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201050

Interrelationship between serum and sputum inflammatory mediators in chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Bizeto,L.; Mazzolini,A.B.; Ribeiro,M.; Stelmach,R.; Cukier,A.; Nunes,M.P.T.
Little is known about airway inflammatory markers in chronic obstructive pulmonary disease (COPD). The objective of the present study was to identify and try to correlate pulmonary and peripheral blood inflammatory markers in COPD. In a cross-sectional study on patients with stable COPD, induced sputum and blood samples were collected for the determination of C-reactive protein, eosinophilic cationic protein, serum amyloid A protein, a-1 antitrypsin (a-1AT), and neutrophil elastase. Twenty-two patients were divided into two groups according to post-bronchodilator forced expiratory volume in the first second (%FEV1): group 1 (N = 12, FEV1 &lt;40%) and group 2 (N = 10, FEV1 ³40%). An increase in serum elastase, eosinophilic cationic protein and a-1AT was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; C-reactive protein; Eosinophilic cationic protein; Serum amyloid A protein; A-1 antitrypsin; Neutrophil elastase.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300003

Purine nucleotides reduce superoxide production by nitric oxide synthase in a murine sepsis model
Provedor de dados: 56
Autores: Barbeiro,H.V.; Barbeiro,D.F.; Debbas,V.; Souza,H.P.; Laurindo,F.R.; Velasco,I.T.; Soriano,F.G.
Sepsis involves a systemic inflammatory response of multiple endogenous mediators, resulting in many of the injurious and sometimes fatal physiological symptoms of the disease. This systemic activation leads to a compromised vascular response and endothelial dysfunction. Purine nucleotides interact with purinoceptors and initiate a variety of physiological processes that play an important role in maintaining cardiovascular function. The purpose of the present study was to investigate the effects of ATP on vascular function in a lipopolysaccharide (LPS) model of sepsis. LPS induced a significant increase in aortic superoxide production 16 h after injection. Addition of ATP to the organ bath incubation solution reduced superoxide production by the aortas of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ATP; Endotoxin; Endothelium; Superoxide; Nitric oxide.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100009

Relationship between cardiovascular dysfunction and hyperglycemia in streptozotocin-induced diabetes in rats
Provedor de dados: 56
Autores: Schaan,B.D.; Dall'Ago,P.; Maeda,C.Y.; Ferlin,E.; Fernandes,T.G.; Schmid,H.; Irigoyen,M.C.
Streptozotocin (STZ)-induced diabetes in rats is characterized by cardiovascular dysfunction beginning 5 days after STZ injection, which may reflect functional or structural autonomic nervous system damage. We investigated cardiovascular and autonomic function, in rats weighing 166 ± 4 g, 5-7, 14, 30, 45, and 90 days after STZ injection (N = 24, 33, 27, 14, and 13, respectively). Arterial pressure (AP), mean AP (MAP) variability (standard deviation of the mean of MAP, SDMMAP), heart rate (HR), HR variability (standard deviation of the normal pulse intervals, SDNN), and root mean square of successive difference of pulse intervals (RMSSD) were measured. STZ induced increased glycemia in diabetic rats vs control rats. Diabetes reduced resting HR from 363 ± 12...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Streptozotocin; Heart rate variability; Rats; Autonomic nervous system; Arterial pressure variability.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200016

Percutaneous sciatic nerve block with tramadol induces analgesia and motor blockade in two animal pain models
Provedor de dados: 56
Autores: Sousa,A.M.; Ashmawi,H.A.; Costa,L.S.; Posso,I.P.; Slullitel,A.
Local anesthetic efficacy of tramadol has been reported following intradermal application. Our aim was to investigate the effect of perineural tramadol as the sole analgesic in two pain models. Male Wistar rats (280-380 g; N = 5/group) were used in these experiments. A neurostimulation-guided sciatic nerve block was performed and 2% lidocaine or tramadol (1.25 and 5 mg) was perineurally injected in two different animal pain models. In the flinching behavior test, the number of flinches was evaluated and in the plantar incision model, mechanical and heat thresholds were measured. Motor effects of lidocaine and tramadol were quantified and a motor block score elaborated. Tramadol, 1.25 mg, completely blocked the first and reduced the second phase of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tramadol; Tramadol-N-oxide; Rats; Peripheral nerves; Local anesthesia; Analgesia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200009

A high-fructose diet induces insulin resistance but not blood pressure changes in normotensive rats
Provedor de dados: 56
Autores: Bezerra,R.M.N.; Ueno,M.; Silva,M.S.; Tavares,D.Q.; Carvalho,C.R.O.; Saad,M.J.A.; Gontijo,J.A.R.
Rats fed a high-fructose diet represent an animal model for insulin resistance and hypertension. We recently showed that a high-fructose diet containing vegetable oil but a normal sodium/potassium ratio induced mild insulin resistance with decreased insulin receptor substrate-1 tyrosine phosphorylation in the liver and muscle of normal rats. In the present study, we examined the mean blood pressure, serum lipid levels and insulin sensitivity by estimating in vivo insulin activity using the 15-min intravenous insulin tolerance test (ITT, 0.5 ml of 6 µg insulin, iv) followed by calculation of the rate constant for plasma glucose disappearance (Kitt) in male Wistar-Hannover rats (110-130 g) randomly divided into four diet groups: control, 1:3 sodium/potassium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-fructose diet; Insulin resistance; Blood pressure; Hypertriglyceridemia; Plasma cholesterol.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900008

Impact of specimen adequacy on the assessment of renal allograft biopsy specimens
Provedor de dados: 56
Autores: Cimen,S.; Geldenhuys,L.; Guler,S.; Imamoglu,A.; Molinari,M.
The Banff classification was introduced to achieve uniformity in the assessment of renal allograft biopsies. The primary aim of this study was to evaluate the impact of specimen adequacy on the Banff classification. All renal allograft biopsies obtained between July 2010 and June 2012 for suspicion of acute rejection were included. Pre-biopsy clinical data on suspected diagnosis and time from renal transplantation were provided to a nephropathologist who was blinded to the original pathological report. Second pathological readings were compared with the original to assess agreement stratified by specimen adequacy. Cohen's kappa test and Fisher's exact test were used for statistical analyses. Forty-nine specimens were reviewed. Among these specimens, 81.6%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal allograft; Biopsy; Specimen adequacy; Banff criteria.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400710

Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
Provedor de dados: 56
Autores: de-Oliveira,G.R.; Gondim,F. de-A.A.; da-Graça,J.R.V.; Xavier-Neto,J.; Dantas,R.P.; Gondim,R.B.M.; Santos,A.A.; Rola,F.H.
The present study evaluates the effect of blood volume expansion on the gastrointestinal transit of a charchoal meal (2.5 ml of an aqueous suspension consisting of 5% charcoal and 5% gum arabic) in awake male Wistar rats (200-270 g). On the day before the experiments, the rats were anesthetized with ether, submitted to left jugular vein cannulation and fasted with water ad libitum until 2 h before the gastrointestinal transit measurement. Blood volume expansion by iv infusion of 1 ml/min Ringer bicarbonate in volumes of 3, 4 or 5% body weight delayed gastrointestinal transit at 10 min after test meal administration by 21.3-26.7% (P&amp;lt;0.05), but no effect was observed after 1 or 2% body weight expansion. The effect of blood volume expansion (up to 5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypervolemia; Blood volume; Gastrointestinal transit; Rats; Gastrointestinal motility.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600017

Cardiovascular risk factors in a population of Brazilian schoolchildren
Provedor de dados: 56
Autores: Rodrigues,A.N.; Moyses,M.R.; Bissoli,N.S.; Pires,J.G.P.; Abreu,G.R.
Epidemiological and clinical evidence suggests that a judicious diet, regular physical activity and blood pressure (BP) monitoring must start in early childhood to minimize the impact of modifiable cardiovascular risk factors. This study was designed to evaluate BP and metabolic parameters of schoolchildren from Vitória, Espírito Santo State, Brazil, and correlate them with cardiovascular risk factors. The study was conducted on 380 students aged 10-14 years (177 boys, 203 girls) enrolled in public schools. Baseline measurements included body mass index, BP and heart rate. The students were submitted to exercise spirometry on a treadmill. VO2max was obtained from exercise testing to voluntary exhaustion. Fasting serum total cholesterol (TC), LDL-C, HDL-C,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular risk factors; Arterial hypertension; Primary prevention; Coronary artery disease; Obesity; Schoolchildren.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200015

Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer
Provedor de dados: 56
Autores: Xu,Li; Peng,Hui; Huang,Xiao-Xu; Xia,Ya-Bin; Hu,Kai-Feng; Zhang,Zheng-Ming.
Previous studies suggested that chromodomain helicase DNA-binding proteins (CHDs), including CHD 1–8, were associated with several human diseases and cancers including lymphoma, liver cancer, colorectal cancer, stomach cancer, etc. To date, little research on CHD 9 in human cancers has been reported. In this study, we assessed the prognostic value of CHD 9 in patients with colorectal cancer (CRC). We screened for CHD 9 expression using immunohistochemical analysis in 87 surgical CRC specimens and found that the expression was upregulated in 81.5% of the cases, while 7.4% were decreased; in the remaining 11.1% of the cases, levels were not altered. Kaplan-Meier analysis showed that patients with high CHD 9 expression had better prognosis than those with low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromodomain helicase DNA-binding protein 9; Colorectal cancer; Immunohistochemical analysis; Biomarker; Prognosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900612

Roles of mitogen-activated protein kinases and angiotensin II in renal development
Provedor de dados: 56
Autores: Balbi,A.P.C.; Francescato,H.D.C.; Marin,E.C.S.; Costa,R.S.; Coimbra,T.M.
Experimental and clinical evidence suggests that angiotensin II (AII) participates in renal development. Renal AII content is several-fold higher in newborn rats and mice than in adult animals. AII receptors are also expressed in higher amounts in the kidneys of newborn rats. The kidneys of fetuses whose mother received a type 1 AII receptor (AT1) antagonist during gestation present several morphological alterations. Mutations in genes that encode components of the renin-angiotensin system are associated with autosomal recessive renal tubular dysgenesis. Morphological changes were detected in the kidneys of 3-week-old angiotensin-deficient mice. Mitogen-activated protein kinases (MAPKs) are important mediators that transduce extracellular stimuli to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal development; Angiotensin II; Mitogen-activated protein kinase; Angiotensin II antagonists; Sodium intake.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100007

Monitoring human cytomegalovirus viral load in peripheral blood leukocytes of renal transplant recipients by a simple limiting dilution-PCR assay
Provedor de dados: 56
Autores: Caballero,O.L.; Costa,M.C.S.L.; Trevisan,A.; Oliveira,R.M.; Viotti,E.A.; Távora,E.R.F.; Vilaça,S.S.; Sabagga,E.; de-Paula,F.J.; Távora,P.F.; Brasileiro-Filho,G.; Villa,L.L.; Simpson,A.J.G.
To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reaction (PCR). We evaluated this assay prospectively in 52 renal transplant patients from whom a total of 495 serial blood samples were collected. The samples scored HCMV positive by qualitative PCR had the levels of HCMV DNA determined by end-point dilution-PCR. All patients were HCMV DNA positive during the monitoring period and a diagnosis of symptomatic infection was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Human cytomegalovirus; Renal transplant; Diagnosis; Viral load.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200010

Effect of carotid and aortic baroreceptors on cardiopulmonary reflex: the role of autonomic function
Provedor de dados: 56
Autores: Fernandes,T.L.; Piratello,A.C.; Farah,V.; Fiorino,P.; Moreira,E.D.; Irigoyen,M.C.; Krieger,E.M.
We determined the sympathetic and parasympathetic control of heart rate (HR) and the sensitivity of the cardiopulmonary receptors after selective carotid and aortic denervation. We also investigated the participation of the autonomic nervous system in the Bezold-Jarish reflex after selective removal of aortic and carotid baroreceptors. Male Wistar rats (220-270 g) were divided into three groups: control (CG, N = 8), aortic denervation (AG, N = 5) and carotid denervation (CAG, N = 9). AG animals presented increased arterial pressure (12%) and HR (11%) compared with CG, while CAG animals presented a reduction in arterial pressure (16%) and unchanged HR compared with CG. The sequential blockade of autonomic effects by atropine and propranolol indicated a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carotid denervation; Aortic denervation; Bezold-Jarish reflex.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700011

Cyclic AMP increases the survival of ganglion cells in mixed retinal cell cultures in the absence of exogenous neurotrophic molecules, an effect that involves cholinergic activity
Provedor de dados: 56
Autores: Santos,R.C.C.; Araujo,E.G.
Natural cell death is a well-known degenerative phenomenon occurring during development of the nervous system. The role of trophic molecules produced by target and afferent cells as well as by glial cells has been extensively demonstrated. Literature data demonstrate that cAMP can modulate the survival of neuronal cells. Cultures of mixed retinal cells were treated with forskolin (an activator of the enzyme adenylyl cyclase) for 48 h. The results show that 50 µM forskolin induced a two-fold increase in the survival of retinal ganglion cells (RGCs) in the absence of exogenous trophic factors. This effect was dose dependent and abolished by 1 µM H89 (an inhibitor of protein kinase A), 1.25 µM chelerythrine chloride (an inhibitor of protein kinase C), 50 µM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ganglion cell survival; CAMP; Amacrine cholinergic cells; M1 receptor activity; Glial cells.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200011

Short-term effects of stored homologous red blood cell transfusion on cardiorespiratory function and inflammation: an experimental study in a hypovolemia model
Provedor de dados: 56
Autores: Biagini,S.; Dale,C.S.; Real,J.M.; Moreira,E.S.; Carvalho,C.R.R.; Schettino,G.P.P.; Wendel,S.; Azevedo,L.C.P.
The pathophysiological mechanisms associated with the effects of red blood cell (RBC) transfusion on cardiopulmonary function and inflammation are unclear. We developed an experimental model of homologous 14-days stored RBC transfusion in hypovolemic swine to evaluate the short-term effects of transfusion on cardiopulmonary system and inflammation. Sixteen healthy male anesthetized swine (68±3.3 kg) were submitted to controlled hemorrhage (25% of blood volume). Two units of non-filtered RBC from each animal were stored under blood bank conditions for 14 days. After 30 min of hypovolemia, the control group (n=8) received an infusion of lactated Ringer's solution (three times the removed volume). The transfusion group (n=8) received two units of homologous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine; Transfusion; Inflammation; Red blood cells; Hypovolemia; Hemorrhage.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100604

Genetic potential for an acute inflammatory response in IgA glomerulonephritis in mice
Provedor de dados: 56
Autores: Kurihara,R.S.; Yokoo,M.; Domingues,W.V.; Cabrera,W.H.; Ribeiro,O.G.; Ibañez,O.M.; Malheiros,D.A.; Barros,R.T.; de Almeida Prado,E.B.
Mice selected on the basis of an acute inflammatory response (AIR) can provide information about the immunopathological mechanisms of glomerulonephritis. We studied the differences between mice selected for a maximal AIR (AIRmax that attract more polymorphonuclear cells to the site of injury) or a minimal AIR (AIRmin that attract more mononuclear cells) in an experimental model of IgA nephropathy in order to investigate the effect of genetic background on glomerular disease progression and the participation of the monocyte chemoattractant protein-1 (MCP-1) chemokine. IgA nephropathy was induced by intraperitoneal ovalbumin injection and bile duct ligation in AIRmax and AIRmin mice. Histological changes, urinary protein/creatinine ratio, serum IgA levels,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocyte chemoattractant protein-1; IgA; Glomerulonephritis; Acute inflammatory response; Chemokine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200009

Frequency of 8 CFTR gene mutations in cystic fibrosis patients in Minas Gerais, Brazil, diagnosed by neonatal screening
Provedor de dados: 56
Autores: Perone,C.; Medeiros,G.S.; del Castillo,D.M.; de Aguiar,M.J.B.; Januário,J.N.
The nature and frequency of cystic fibrosis mutations in Brazil is not uniform due to the highly varied ethnic composition of the population. The average frequency of the F508del mutation has been reported to be 48.6%. Other common mutations in Brazil are G542X, R1162X, and N1303K. The aim of this study was to analyze the frequency of 8 mutations (F508del, G542X, R1162X, N1303K, W1282X, G85E, 3120+1G&gt;A, and 711+1G&gt;T) in a sample of 111 newborn patients with cystic fibrosis diagnosed by the Cystic Fibrosis Neonatal Screening Program of Minas Gerais State. The mutations were tested by allele-specific oligonucleotide PCR with specially designed primers. An allele frequency of 48.2% was observed for the F508del mutation, and allele frequencies of 5.41,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CFTR gene; Cystic fibrosis; Mutations; Neonatal screening; F508del.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200003

Leptin is not associated independently with hypertension in Japanese-Brazilian women
Provedor de dados: 56
Autores: Almeida-Pititto,B.; Gimeno,S.G.A.; Freire,R.D.; Ribeiro-Filho,F.F.; Ferreira,S.R.G.
We evaluated the relationship of leptin with hypertension adjusted for body mass index (BMI) and/or waist circumference in a population of Japanese-Brazilian women aged  &gt; or = 30 years with centrally distributed adiposity. After excluding diabetic subjects, the study subjects - who participated in a population-based study on the prevalence of metabolic syndrome - showed prevalence rates of obesity (BMI  &gt; or = 25 kg/m²) and central adiposity (waist  &gt; or = 80 cm) of 32.0 and 37.8%, respectively. The hypertensive group (N = 162) was older, had higher BMI (24.9 ± 4.2 vs 23.3 ± 3.4 kg/m², P &lt; 0.001), waist circumference (81.1 ± 10.1 vs 76.3 ± 8.2 cm, P &lt; 0.001) and insulin levels (8.0 ± 6.2 vs 7.1 ± 4.9 µU/mL, P &lt; 0.05) than the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Body fat distribution; Insulin; Leptin; Centrally obese; Metabolic syndrome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100012

Symmetric pollen mitosis I and suppression of pollen mitosis II prevent pollen development in Brachiaria jubata (Gramineae)
Provedor de dados: 56
Autores: Risso-Pascotto,C.; Pagliarini,M.S.; Valle,C.B.; Jank,L.
Microsporogenesis and pollen development were analyzed in a tetraploid (2n = 4x = 36) accession of the forage grass Brachiaria jubata (BRA 007820) from the Embrapa Beef Cattle Brachiaria collection that showed partial male sterility. Microsporocytes and pollen grains were prepared by squashing and staining with 0.5% propionic carmine. The meiotic process was typical of polyploids, with precocious chromosome migration to the poles and laggards in both meiosis I and II, resulting in tetrads with micronuclei in some microspores. After callose dissolution, microspores were released into the anther locule and appeared to be normal. Although each microspore initiated its differentiation into a pollen grain, in 11.1% of them nucleus polarization was not observed,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachiaria jubata; Male sterility; Microgametogenesis; Pollen mitosis; Sperm cell.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100006

Recombinant viruses as vaccines against viral diseases
Provedor de dados: 56
Autores: Souza,A.P.D.; Haut,L.; Reyes-Sandoval,A.; Pinto,A.R.
Vaccine approaches to infectious diseases are widely applied and appreciated. Amongst them, vectors based on recombinant viruses have shown great promise and play an important role in the development of new vaccines. Many viruses have been investigated for their ability to express proteins from foreign pathogens and induce specific immunological responses against these antigens in vivo. Generally, gene-based vaccines can stimulate potent humoral and cellular immune responses and viral vectors might be an effective strategy for both the delivery of antigen-encoding genes and the facilitation and enhancement of antigen presentation. In order to be utilized as a vaccine carrier, the ideal viral vector should be safe and enable efficient presentation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccine; Immunity; Recombinant viruses; Viral vectors; Infectious disease.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400004

lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer
Provedor de dados: 56
Autores: Song,Linlin; Wang,Linlin; Pan,Xiaoli; Yang,Caihong.
Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarified that ROCK1 was the downstream effector of OIP5-AS1 and OIP5-AS1 acted as a molecular sponge of miR-143-3p. Finally, we verified that OIP5-AS1 exerted its function in the regulation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA; OIP5-AS1; Cervical cancer; MiR-143-3p; ROCK1.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100608

Contrasting effects of nitric oxide and corticotropin- releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in mice
Provedor de dados: 56
Autores: Miguel,T.T.; Gomes,K.S.; Nunes-de-Souza,R.L.
The anxiogenic and antinociceptive effects produced by glutamate N-methyl-D-aspartate receptor activation within the dorsal periaqueductal gray (dPAG) matter have been related to nitric oxide (NO) production, since injection of NO synthase (NOS) inhibitors reverses these effects. dPAG corticotropin-releasing factor receptor (CRFr) activation also induces anxiety-like behavior and antinociception, which, in turn, are selectively blocked by local infusion of the CRF type 1 receptor (CRFr1) antagonist, NBI 27914 [5-chloro-4-(N-(cyclopropyl)methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)aminopyridine]. Here, we determined whether i) the blockade of the dPAG by CRFr1 attenuates the anxiogenic/antinociceptive effects induced by local infusion of the NO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Corticotropin-releasing factor; Periaqueductal gray; Defensive behavior; Antinociception; Mice.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400003

Do Caucasian and Asian clocks tick differently?
Provedor de dados: 56
Autores: Barbosa,A.A.; Pedrazzoli,M.; Koike,B.D.V.; Tufik,S.
The Period 3 and Clock genes are important components of the mammalian molecular circadian system. Studies have shown association between polymorphisms in these clock genes and circadian phenotypes in different populations. Nevertheless, differences in the pattern of allele frequency and genotyping distribution are systematically observed in studies with different ethnic groups. To investigate and compare the pattern of distribution in a sample of Asian and Caucasian populations living in Brazil, we evaluated two well-studied polymorphisms in the clock genes: a variable number of tandem repeats (VNTR) in PER3 and a single nucleotide polymorphism (SNP) in CLOCK. The aim of this investigation was to search for clues about human evolutionary processes related...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: PER3 gene; CLOCK gene; Circadian rhythms; Asian; Caucasian; Ethnic.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100013

Effects of small doses of ouabain on the arterial blood pressure of anesthetized hypertensive and normotensive rats
Provedor de dados: 56
Autores: Rossoni,L.V.; Pinto,V.D.; Vassallo,D.V.
Ouabain increases vascular resistance and may induce hypertension by inhibiting the Na+ pump. The effects of 0.18 and 18 µg/kg, and 1.8 mg/kg ouabain pretreatment on the phenylephrine (PHE; 0.1, 0.25 and 0.5 µg, in bolus)-evoked pressor responses were investigated using anesthetized normotensive (control and uninephrectomized) and hypertensive (1K1C and DOCA-salt treated) rats. Treatment with 18 µg/kg ouabain increased systolic and diastolic blood pressure in all groups studied. However, the magnitude of this increase was larger for the hypertensive 1K1C and DOCA-salt rats than for normotensive animals, while the pressor effect of 0.18 µg/kg ouabain was greater only in DOCA-salt rats. A very large dose (1.8 mg/kg) produced toxic effects on the normotensive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pressor reactivity; Ouabain; Phenylephrine; 1K1C rats; DOCA-salt; Uninephrectomy.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800014

Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor
Provedor de dados: 56
Autores: Xu,H.H.; Liu,S.H.; Guo,Q.F.; Liu,Q.H.; Li,X.Y.
Bone morphogenetic protein 2 (BMP2) and basic fibroblast growth factor (bFGF) have been shown to exhibit a synergistic effect to promote bone repair and healing. In this study, we constructed a novel adenovirus with high coexpression of BMP2 and bFGF and evaluated its effect on osteogenic differentiation of goat bone marrow progenitor cells (BMPCs). Recombinant adenovirus Ad-BMP2-bFGF was constructed by using the T2A sequence. BMPCs were isolated from goats by density gradient centrifugation and adherent cell culture, and were then infected with Ad-BMP2-bFGF or Ad-BMP2. Expression of BMP2 and bFGF was detected by ELISA, and alkaline phosphatase (ALP) activity was detected by an ALP assay kit. In addition, von Kossa staining and immunocytochemical staining...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BMP2; BFGF; Adenovirus; T2A sequence; BMPCs; Osteogenic differentiation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900809

The size and prevalence of the cavum septum pellucidum are normal in subjects with panic disorder
Provedor de dados: 56
Autores: Crippa,J.A.S.; Uchida,R.; Busatto,G.F.; Guimarães,F.S.; Del-Ben,C.M.; Zuardi,A.W.; Santos,A.C.; Araújo,D.; McGuire,P.K.; Graeff,F.G.
Panic disorder is thought to involve dysfunction in the septohippocampal system, and the presence of a cavum septum pellucidum might indicate the aberrant development of this system. We compared the prevalence and size of cavum septum pellucidum in 21 patients with panic disorder and in 21 healthy controls by magnetic resonance imaging. The length of the cavum septum pellucidum was measured by counting the number of consecutive 1-mm coronal slices in which it appeared. A cavum septum pellucidum of &gt;6 mm in length was rated as large. There was no significant difference in the proportion of patients (16 of 21 or 76.2%) and controls (18 of 21 or 85.7%) with a cavum septum pellucidum (P = 0.35, Fisher's exact test, one-tailed), and no members of either...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cavum septum pellucidum; Panic disorder; Neuroimaging; Magnetic resonance imaging.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300013

Postural balance in patients with social anxiety disorder
Provedor de dados: 56
Autores: Levitan,M.N.; Crippa,J.A.; Bruno,L.M.; Pastore,D.L.; Freire,R.C.; Arrais,K.C.; Hallak,J.E.; Nardi,A.E.
Body stability is controlled by the postural system and can be affected by fear and anxiety. Few studies have addressed freezing posture in psychiatric disorders. The purpose of the present study was to assess posturographic behavior in 30 patients with social anxiety disorder (SAD) and 35 without SAD during presentation of blocks of pictures with different valences. Neutral images consisted of objects taken from a catalog of pictures, negative images were mutilation pictures and anxiogenic images were related to situations regarding SAD fears. While participants were standing on a force platform, similar to a balance, displacement of the center of pressure in the mediolateral and anteroposterior directions was measured. We found that the SAD group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Social anxiety disorder; Postural control; Freezing; Stabilometry; Anxiety; Social exposure.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100007

Interference of propylene glycol with the hole-board test
Provedor de dados: 56
Autores: Lourenço da Silva,A.; Elisabetsky,E.
Experimental drugs and/or plant extracts are often dissolved in solvents, including propylene glycol. Nevertheless, there is evidence for psychoactive properties of this alcohol. In this study we found that in the hole-board test 10% propylene glycol did not modify the head-dipping behavior. However, 30% propylene glycol induced an increase in the number of head-dips (46.92 ± 2.37 compared to 33.83 ± 4.39, P&lt;0.05, ANOVA/Student-Newman-Keuls), an effect comparable to that obtained with 0.5 mg/kg diazepam (from 33.83 ± 4.39 to 54 ± 3.8, P&lt;0.01, ANOVA/Student-Newman-Keuls). These results demonstrate that 30% propylene glycol has significant anxiolytic effects in this model and therefore cannot be used as an innocuous solvent.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Screening; Anxiolytic drugs; Propylene glycol; Diazepam; Solvents; Hole-board.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400016

Short-term and long-term antibody response by mice after immunization against Neisseria meningitidis B or diphtheria toxoid
Provedor de dados: 56
Autores: Silva,G.P.; Cruz,S.C.; Cruz,A.C.; Milagres,L.G.
Serogroup B Neisseria meningitidis (MenB) is a major cause of invasive disease in early childhood worldwide. The only MenB vaccine available in Brazil was produced in Cuba and has shown unsatisfactory efficacy when used to immunize millions of children in Brazil. In the present study, we compared the specific functional antibody responses evoked by the Cuban MenB vaccine with a standard vaccine against diphtheria (DTP: diphtheria, tetanus, pertussis) after primary immunization and boosting of mice. The peak of bactericidal and opsonic antibody titers to MenB and of neutralizing antibodies to diphtheria toxoid (DT) was reached after triple immunization with the MenB vaccine or DTP vaccine, respectively. However, 4 months after immunization, protective DT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serological memory; Antibody recall response; Booster immunization.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200148

Responsiveness of glycogen breakdown to cyclic AMP in perfused liver from rats with insulin-induced hypoglycemia
Provedor de dados: 56
Autores: Vardanega-Peicher,M.; Curi,R.; Pagliarini e Silva,S.; Nascimento,K.F.; Bazotte,R.B.
The responsiveness of glycogen breakdown to cAMP was investigated in isolated perfused liver from male Wistar fed rats (200-220 g) with insulin-induced hypoglycemia. The activation of glycogenolysis by 3 µM cAMP was decreased (P&lt;0.05) in livers from rats with hypoglycemia induced by the administration of insulin or during the direct infusion of insulin into the isolated liver. The direct effect of insulin on glycogen catabolism promoted by 3 µM cAMP occurred as early as 3 min after starting insulin infusion. In contrast, the cAMP agonists resistant to phosphodiesterases, 8Br-cAMP and 6MB-cAMP, used at the same concentration as cAMP, i.e., 3 µM, did not modify the effect of insulin. The data suggest that the decreased hepatic responsiveness of glycogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin-induced hypoglycemia; Cyclic AMP; Glycogen breakdown; Liver metabolism.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100007

Plasma concentrations and placental immunostaining of interleukin-10 and tumornecrosis factor-α as predictors of alterations in the embryo-fetal organism and the placental development of diabetic rats
Provedor de dados: 56
Autores: Sinzato,Y.K.; Damasceno,D.C.; Laufer-Amorim,R.; Rodrigues,M.M.P.; Oshiiwa,M.; Taylor,K.N.; Rudge,M.V.C.
Interleukin-10 (IL-10) appears to be the key cytokine for the maintenance of pregnancy and inhibits the secretion of inflammatory cytokines such as tumor necrosis factor-α (TNF-α). However, there are no studies evaluating the profile of these cytokines in diabetic rat models. Thus, our aim was to analyze IL-10 and TNF-α immunostaining in placental tissue and their respective concentrations in maternal plasma during pregnancy in diabetic rats in order to determine whether these cytokines can be used as predictors of alterations in the embryo-fetal organism and in placental development. These parameters were evaluated in non-diabetic (control; N = 15) and Wistar rats with streptozotocin (STZ)-induced diabetes (N = 15). At term, the dams (100 days of life)...
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300005

Evidence of interaction between fluoxetine and isosorbide dinitrate on neuroleptic-induced catalepsy in mice
Provedor de dados: 56
Autores: Pires,J.G.P.; Fonseca,F.C.G.; Woelffel,A.B.; Futuro-Neto,H.A.
Drugs which influence 5-HTergic mechanisms can modify neuroleptic-induced catalepsy (NC) in rodents, a phenomenon produced by striatal dopamine (DA) receptor blockade. Previous research also suggests a role for endogenous nitric oxide (NO) in the modulation of striatal DAergic neurotransmission; in addition, NO seems to play a role in the 5-HT reuptake mechanism. It is known that clomipramine potentiates NC in mice, but the reported effects of selective 5-HT reuptake inhibitors (SSRIs) in this model are rather contradictory. We then decided to re-address this issue, investigating the effect of fluoxetine (FX), an SSRI, on NC. In view of the ubiquitous role of NO as a central neuromodulator, we also studied the effect of isosorbide dinitrate (ID), a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoxetine; Isosorbide dinitrate; Neuroleptic-induced catalepsy; Nitric oxide; Serotonin reuptake inhibitors; Dopaminergic transmission.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300014

Effect of fasudil on experimental autoimmune neuritis and its mechanisms of action
Provedor de dados: 56
Autores: Zhao,Yanyin; Liu,Bingyou; Wang,Yi; Xiao,Baoguo.
This study aimed to investigate the therapeutic effect of fasudil on treating experimental autoimmune neuritis (EAN). Twenty-four EAN mice were randomly assigned to fasudil treatment (Fasudil group) or saline treatment (EAN model group) for 28 days. Clinical symptom score was evaluated every other day; inflammatory cell infiltration, demyelination, anti-myelin basic protein (MBP), inflammatory cytokines, inducible nitric oxide synthase (iNOS), and arginase-1 were detected in sciatic nerves at day 28. Th1, Th2, Th17, and Tregs proportions in splenocytes were detected at day 28. Clinical symptom score was found to be attenuated in the Fasudil group compared to the EAN model group from day 12 to day 28. Sciatic nerve inflammatory cell counts by HE staining...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fasudil; Experimental autoimmune neuritis; Th1; Th17; Tregs; M2 macrophages polarization.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100607

Glutamic acid decarboxylase antibodies are indicators of the course, but not of the onset, of diabetes in middle-aged adults: the Atherosclerosis Risk in Communities Study
Provedor de dados: 56
Autores: Vigo,A.; Duncan,B.B.; Schmidt,M.I.; Couper,D.; Heiss,G.; Pankow,J.S.; Ballantyne,C.M.
To efficiently examine the association of glutamic acid decarboxylase antibody (GADA) positivity with the onset and progression of diabetes in middle-aged adults, we performed a case-cohort study representing the ~9-year experience of 10,275 Atherosclerosis Risk in Communities Study participants, initially aged 45-64 years. Antibodies to glutamic acid decarboxylase (GAD65) were measured by radioimmunoassay in 580 incident diabetes cases and 544 non-cases. The overall weighted prevalence of GADA positivity (³1 U/mL) was 7.3%. Baseline risk factors, with the exception of smoking and interleukin-6 (P £ 0.02), were generally similar between GADA-positive and -negative individuals. GADA positivity did not predict incident diabetes in multiply adjusted (HR =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Glutamic acid decarboxylase; Auto-immune diseases; Inflammation; Risk factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700007

Effect of hyperbaric oxygenation on the regeneration of the liver after partial hepatectomy in rats
Provedor de dados: 56
Autores: Kurir,T.T.; Markotic,A.; Katalinic,V.; Bozanic,D.; Cikes,V.; Zemunik,T.; Modun,D.; Rincic,J.; Boraska,V.; Bota,B.; Salamunic,I.; Radic,S.
The aim of the present study was to assess the influence of hyperbaric oxygenation (HBO) on rat liver regeneration before and after partial hepatectomy. Rats were sacrificed 54 h after 15% hepatectomy, liver and body weights were measured, and serum alanine transaminase (ALT) and aspartate transaminase (AST) activity and albumin levels were determined. The lipid peroxide level, as indicated by malondialdehyde production in the remnant liver was measured, and liver sections were analyzed by light microscopy. Five groups of 10 rats in each group were studied. The preHBO and pre-hyperbaric pressure (preHB) groups were treated before partial hepatectomy with 100% O2 and 21% O2, respectively, at 202,650 pascals, daily for 3 days (45 min/day). The control group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partial hepatectomy; Hyperbaric oxygenation; Liver regeneration; Alanine transaminase; Aspartate transaminase.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800014

Acute exercise inhibits gastric emptying of liquids in rats: influence of the NO-cGMP pathway
Provedor de dados: 56
Autores: Cavalcante,A.K.M.; Siqueira,R.C.L.; Feitosa Júnior,V.N.; de Andrade,C.R.; Santos,A.A.; Silva,M.T.B.
We previously found that acute exercise inhibited the gastric emptying of liquid in awake rats by causing an acid-base imbalance. In the present study, we investigated the involvement of the nitric oxide-cyclic guanosine monophosphate (NO-cGMP) pathway, vasoactive intestinal peptide (VIP), and corticotropin-releasing factor (CRF) peptide in this phenomenon. Male rats were divided into exercise or sedentary group and were subjected to a 15-min swim session against a load (2.5 or 5% b.w.). The rate of gastric emptying was evaluated after 5, 10, or 20 min postprandially. Separate groups of rats were treated with vehicle (0.9% NaCl, 0.1 mL/100 g, ip) or one of the following agents: atropine (1.0 mg/kg, ip), the NO non-selective inhibitor Nω-nitro-L-arginine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute exercise; Bioelectrical impedance; Gastric motility; High-intensity exercise.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100607

The role of complement in the early phase of Leishmania (Leishmania) amazonensis infection in BALB/c mice
Provedor de dados: 56
Autores: Laurenti,M.D.; Örn,A.; Sinhorini,I.L.; Corbett,C.E.P.
Complement-depleted and -non-depleted BALB/c mice were inoculated with Leishmania (Leishmania) amazonensis promastigotes into the hind footpad to study the role of the complement system in cutaneous leishmaniasis. Total serum complement activity was measured by hemolytic assay and C3 fragment deposit at the inoculation site was determined by direct immunofluorescence in the early period of infection, i.e., at 3, 24, 48 h and 7 days post-infection. The inflammatory reaction and the parasite burden were evaluated in the skin lesion at 7 and 30 days post-infection. Total serum complement activity decreased in the early phase of infection, from 3 to 24 h, in non-depleted mice compared to non-infected and non-depleted mice. C3 fragment deposit at the site of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Leishmania (Leishmania) amazonensis; Complement; Pathology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300021

A chromatographic method for the production of a human immunoglobulin G solution for intravenous use
Provedor de dados: 56
Autores: Tanaka,K.; Sawatani,E.; Shigueoka,E.M.; Campos,T.C.X.B.; Nakao,H.C.; Dias,G.A.; Fujita,R.K.; Arashiro,F.
Immunoglobulin G (IgG) of excellent quality for intravenous use was obtained from the cryosupernatant of human plasma by a chromatographic method based on a mixture of ion-exchange, DEAE-Sepharose FF and arginine Sepharose 4B affinity chromatography and a final purification step by Sephacryl S-300 HR gel filtration. The yield of 10 experimental batches produced was 3.5 g IgG per liter of plasma. A solvent/detergent combination of 1% Tri (n-butyl) phosphate and 1% Triton X-100 was used to inactivate lipid-coated viruses. Analysis of the final product (5% liquid IgG) based on the mean for 10 batches showed 94% monomers, 5.5% dimers and 0.5% polymers and aggregates. Anticomplementary activity was 0.3 CH50/mg IgG and prekallikrein activator levels were less...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunoglobulin G production; Hemoderivate production; Intravenous gamma globulin production; Industrial chromatography; Downstream process.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100002

The anesthetic agent sevoflurane attenuates pulmonary acute lung injury by modulating apoptotic pathways
Provedor de dados: 56
Autores: Wang,L.; Ye,Y.; Su,H.B.; Yang,J.P.
The objective of this study was to evaluate lung protection by the volatile anesthetic sevoflurane (SEVO), which inhibits apoptosis. Male Sprague-Dawley rats (250–280 g; n=18) were randomly divided into three groups. The LPS group received 5 mg/kg endotoxin (lipopolysaccharide), which induced acute lung injury (ALI). The control (CTRL) group received normal saline and the SEVO group received sevoflurane (2.5%) for 30 min after ALI was induced by 5 mg/kg LPS. Samples were collected for analysis 12 h after LPS. Lung injury was assessed by pathological observations and tissue wet to dry weight (W/D) ratios. Apoptotic index (AI) was determined by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and electron microscopy. Caspase-3 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sevofluran; Apoptosis; Pulmonary acute lung injury; Caspase-3; Bcl-2.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300702

How auditory temporal processing deficits relate to dyslexia
Provedor de dados: 56
Autores: Murphy,C.F.B.; Schochat,E.
Studies have shown that dyslexic children present a deficiency in the temporal processing of auditory stimuli applied in rapid succession. However, discussion continues concerning the way this deficiency can be influenced by temporal variables of auditory processing tests. Therefore, the purpose of the present study was to analyze by auditory temporal processing tests the effect of temporal variables such as interstimulus intervals, stimulus duration and type of task on dyslexic children compared to a control group. Of the 60 children evaluated, 33 were dyslexic (mean age = 10.5 years) and 27 were normal controls (mean age = 10.8 years). Auditory processing tests assess the abilities of discrimination and ordering of stimuli in relation to their duration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Auditory temporal processing; Dyslexia; Hearing tests; Children.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700009

Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro
Provedor de dados: 56
Autores: Wang,Z.G.; Yu,S.D.; Xu,Z.R.
The objective of the present study was to examine the effect of green tea polyphenols (GTPs) supplementation during in vitro maturation, in vitro fertilization, and in vitro culture on the developmental competence of bovine oocytes. Cumulus-oocyte complexes aspirated from the ovaries were matured in vitro (38.5ºC for 24 h) and fertilized (38.5ºC for 15-18 h) and embryos were cultured (38.5ºC for 192 h) in a defined conditioned medium with or without GTPs supplementation. The GTPs used in the present study contained 99% catechin derivatives, with the major components being 50% (-)-epigallocatechin gallate, 22% (-)-epicatechin gallate, 18% (-)-epigallocatechin, and 10% (-)-epicatechin. Four replicate trials were done for each type of experiment. GTPs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine oocytes; In vitro culture; Green tea polyphenols; Embryo development.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800008

Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface
Provedor de dados: 56
Autores: Li,N.; Wu,H.M.; Hang,F.; Zhang,Y.S.; Li,M.J.
Immunological mechanisms have been proposed to underlie the pathogenesis of recurrent spontaneous abortion (RSA). Vitamin D has a potent immunomodulatory effect, which may affect pregnancy outcome. The objective of this study was to investigate 25-hydroxyvitamin D [25(OH) D] concentration and vitamin D receptor (VDR) expression in the decidual tissues of RSA patients. Thirty women with RSA (RSA group) and thirty women undergoing elective abortion (control group) were recruited during 2016 from gynecology outpatient clinics. We measured 25(OH) D, interleukin (IL)-17, IL-23, transforming growth factor β (TGF-β), VDR and 1-α-hydroxylase (CYP27B1) in decidual tissues collected during the abortion procedure. In the RSA group, 25(OH) D and TGF-β were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recurrent spontaneous abortion (RSA); Treg/Th17 cells; Maternal-fetal interface; 25(OH) D; Vitamin D metabolism.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100606

FGFR1 is an independent prognostic factor and can be regulated by miR-497 in gastric cancer progression
Provedor de dados: 56
Autores: Xie,Gang; Ke,Qi; Ji,Yu Zu; Wang,An-qun; Jing,Meng; Zou,li-li.
Fibroblast growth factor receptor 1 (FGFR1) has been reported in gastric cancer to be a prognostic factor. However, miR-497-targeted FGFR1 has not been explored in the carcinogenesis of gastric cancer. The present study intended to revalidate the prognostic significance of FGFR1 in patients with gastric cancer, and the mechanism of miR-497-regulated FGFR1 was investigated in gastric cancer cell proliferation and apoptosis. The messenger RNA (mRNA) and protein levels were assayed by RT-qPCR and western blotting, respectively. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Cell proliferation was analyzed by CCK-8 assay. Annexin V-FITC/PI staining was used to evaluate the apoptosis in AGS and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FGFR1; MiR-497; Apoptosis; Post-transcriptional; Gastric cancer.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100602

Renal actions of angiotensin-(1-7)
Provedor de dados: 56
The heptapeptide angiotensin-(1-7) is considered to be a biologically active endproduct of the renin-angiotensin system. This angiotensin, which is devoid of the most known actions of angiotensin II such as induction of drinking behavior and vasoconstriction, has several selective effects in the brain and periphery. In the present article we briefly review recent evidence for a physiological role of angiotensin-(1-7) in the control of hydroelectrolyte balance
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin antagonists; Angiotensin II; Hydroelectrolyte balance; Renin-angiotensin system; Kidney; Water transport.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400012

Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications
Provedor de dados: 56
Autores: Guidoni,M.; de Christo Scherer,M.M.; Figueira,M.M.; Schmitt,E.F.P.; de Almeida,L.C.; Scherer,R.; Bogusz,S.; Fronza,M.
Vegetable oils have been used for a plethora of health benefits by their incorporation in foods, cosmetics, and pharmaceutical products, especially those intended for skin care. This study aimed to investigate the cutaneous benefits of a vegetable oil blend (VOB) formulation and its fatty acid composition. The anti-inflammatory activity was studied in macrophages of RAW 264.7 cells by investigating the release of nitric oxide (NO), superoxide anion generation (O2-), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6). ABTS cation radical scavenging capacity assay, ferric reducing antioxidant potential (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and NO free radical scavenging assays were used to evaluate the antioxidant activity. VOB was tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetable oil blend; Skin care; Fatty acids; Anti-inflammatory; Nitric oxide; Scratch assay.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200609

Analysis of HLA-A antigens and C282Y and H63D mutations of the HFE gene in Brazilian patients with hemochromatosis
Provedor de dados: 56
Autores: Bittencourt,P.L.; Palácios,S.A.; Couto,C.A.; Cançado,E.L.R.; Carrilho,F.J.; Laudanna,A.A.; Kalil,J.; Gayotto,L.C.C.; Goldberg,A.C.
The hemochromatosis gene, HFE, is located on chromosome 6 in close proximity to the HLA-A locus. Most Caucasian patients with hereditary hemochromatosis (HH) are homozygous for HLA-A3 and for the C282Y mutation of the HFE gene, while a minority are compound heterozygotes for C282Y and H63D. The prevalence of these mutations in non-Caucasian patients with HH is lower than expected. The objective of the present study was to evaluate the frequencies of HLA-A antigens and the C282Y and H63D mutations of the HFE gene in Brazilian patients with HH and to compare clinical and laboratory profiles of C282Y-positive and -negative patients with HH. The frequencies of HLA-A and C282Y and H63D mutations were determined by PCR-based methods in 15 male patients (median...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemochromatosis; HFE mutations; Iron overload; HLA-A3 mutation; C282Y mutation; H63D mutation; Chromosome 6.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300007

Anxiety and depression in parents of a Brazilian non-clinical sample of attention-deficit/ hyperactivity disorder (ADHD) students
Provedor de dados: 56
Autores: Segenreich,D.; Fortes,D.; Coutinho,G.; Pastura,G.; Mattos,P.
Higher prevalence rates of anxiety and depression have been reported in parents of children with attention-deficit/hyperactivity disorder (ADHD). The interaction between the burden of ADHD in offspring, a higher prevalence rate of this highly inherited disorder in parents, and comorbidities may explain this finding. Our objective was to investigate levels of ADHD, anxious and depressive symptomatology, and their relationship in parents of ADHD children from a non-clinical sample using a dimensional approach. The sample included 396 students enrolled in all eight grades of a public school who were screened for ADHD using the SNAP IV rating scale. Positive cases were confirmed through a semi-structured interview. Parents of all 26 ADHD students and 31 paired...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention-deficit/hyperactivity disorder; ADHD; Anxiety; Depression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500011

Chronic experimental myocardial infarction produces antinatriuresis by a renal nerve-dependent mechanism
Provedor de dados: 56
Autores: Souza,D.R.B.; Mill,J.G.; Cabral,A.M.
The present study focused on the role of sympathetic renal nerve activity, in mediating congestive heart failure-induced sodium retention following experimental chronic myocardial infarction. Groups of male Wistar rats (240-260 g) were studied: sham-operated coronary ligation (CON3W, N = 11), coronary ligation and sham-operated renal denervation (INF3W, N = 19), 3 weeks of coronary ligation and sympathetic renal nerve denervation (INF3WDX, N = 6), sham-operated coronary ligation (N = 7), and 16 weeks of coronary ligation (INF16W, N = 7). An acute experimental protocol was used in which the volume overload (VO; 5% of body weight) was applied for 30 min after the equilibration period of continuous iv infusion of saline. Compared to control levels, VO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal denervation; Heart failure; Renal sympathetic nerves; Renal excretory function; Urinary sodium excretion.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200017

Viral membrane fusion: is glycoprotein G of rhabdoviruses a representative of a new class of viral fusion proteins?
Provedor de dados: 56
Autores: Da Poian,A.T.; Carneiro,F.A.; Stauffer,F.
Enveloped viruses always gain entry into the cytoplasm by fusion of their lipid envelope with a cell membrane. Some enveloped viruses fuse directly with the host cell plasma membrane after virus binding to the cell receptor. Other enveloped viruses enter the cells by the endocytic pathway, and fusion depends on the acidification of the endosomal compartment. In both cases, virus-induced membrane fusion is triggered by conformational changes in viral envelope glycoproteins. Two different classes of viral fusion proteins have been described on the basis of their molecular architecture. Several structural data permitted the elucidation of the mechanisms of membrane fusion mediated by class I and class II fusion proteins. In this article, we review a number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Membrane fusion; Fusion proteins; Rhabdovirus; G protein; Glycoprotein.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600002

5-Methyltetrahydrofolate-homocysteine methyltransferase gene polymorphism (MTR) and risk of head and neck cancer
Provedor de dados: 56
Autores: Galbiatti,A.L.S.; Ruiz,M.T.; Biselli-Chicote,P.M.; Raposo,L.S.; Maniglia,J.V.; Pavarino-Bertelli,E.C.; Goloni-Bertollo,E.M.
The functional effect of the A&gt;G transition at position 2756 on the MTR gene (5-methyltetrahydrofolate-homocysteine methyltransferase), involved in folate metabolism, may be a risk factor for head and neck squamous cell carcinoma (HNSCC). The frequency of MTR A2756G (rs1805087) polymorphism was compared between HNSCC patients and individuals without history of neoplasias. The association of this polymorphism with clinical histopathological parameters was evaluated. A total of 705 individuals were included in the study. The polymerase chain reaction-restriction fragment length polymorphism technique was used to genotype the polymorphism. For statistical analysis, the chi-square test (univariate analysis) was used for comparisons between groups and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Head and neck cancer; Polymorphism; Folate metabolism; MTR gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500005

Influence of chronotype and social zeitgebers on sleep/wake patterns
Provedor de dados: 56
Autores: Korczak,A.L.; Martynhak,B.J.; Pedrazzoli,M.; Brito,A.F.; Louzada,F.M.
Inter-individual differences in the phase of the endogenous circadian rhythms have been established. Individuals with early circadian phase are called morning types; those with late circadian phase are evening types. The Horne and Östberg Morningness-Eveningness Questionnaire (MEQ) is the most frequently used to assess individual chronotype. The distribution of MEQ scores is likely to be biased by several fact, ors, such as gender, age, genetic background, latitude, and social habits. The objective of the present study was to determine the effect of different social synchronizers on the sleep/wake cycle of persons with different chronotypes. Volunteers were selected from a total of 1232 UFPR undergraduate students who completed the MEQ. Thirty-two subjects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronotype; Sleep/wake cycle; Morning types; Evening types; Circadian rhythm; Social zeitgebers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000014

Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
Provedor de dados: 56
Autores: Villar,L.M.; Morais,L.M.; Aloise,R.; Melo,M.M.M.; Calado,I.A.; Lampe,E.; Gaspar,A.M.C.
The Northeast region is the location of most cases of acute hepatitis A virus (HAV) in Brazil. In the present study, the genotypes of HAV strains from Pernambuco State, one of most populous states in the Northeast region, were characterized. Blood samples positive for anti-HAV IgM from 145 individuals (mean age = 29.1 years), collected during 2002 and 2003, were submitted to nested RT-PCR for amplification of the 5'non-translated region (5'NTR) and VP1/2A regions of the HAV genome. The VP1/2A and 5'NTR regions were amplified in 39 and 21% of the samples, respectively. Nucleotide sequencing was carried out in 46% of VP1/2A and in 53% of 5'NTR isolates. The identity in nucleotide sequence of the VP1/2A region ranged from 93.6 to 100.0%. Phylogenetic analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis A virus; Molecular epidemiology; Sub-genotype IA; Sub-genotype IB.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700004

Facilitation of the main generator source of earthworm muscle contraction by a peripheral neuron
Provedor de dados: 56
Autores: Chang,Y.C.; Assmé,Z.; Bartoszeck,A.B.
A constant facilitation of responses evoked in the earthworm muscle contraction generator neurons by responses evoked in the neurons of its peripheral nervous system was demonstrated. It is based on the proposal that these two responses are bifurcations of an afferent response evoked by the same peripheral mechanical stimulus but converging again on this central neuron. A single-peaked generator response without facilitation was demonstrated by sectioning the afferent route of the peripheral facilitatory modulatory response, or conditioning response (CR). The multipeaked response could be restored by restimulating the sectioned modulatory neuron with an intracellular substitutive conditioning stimulus (SCS). These multi-peaked responses were proposed to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peri-Kästchen neuron; Facilitation of main generator response; Single and multipeaked responses.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000010

Galactose oxidation using 13C in healthy and galactosemic children
Provedor de dados: 56
Autores: Resende-Campanholi,D.R.; Porta,G.; Ferrioli,E.; Pfrimer,K.; Ciampo,L.A. Del; Junior,J.S. Camelo.
Galactosemia is an inborn error of galactose metabolism that occurs mainly as the outcome of galactose-1-phosphate uridyltransferase (GALT) deficiency. The ability to assess galactose oxidation following administration of a galactose-labeled isotope (1-13C-galactose) allows the determination of galactose metabolism in a practical manner. We aimed to assess the level of galactose oxidation in both healthy and galactosemic Brazilian children. Twenty-one healthy children and seven children with galactosemia ranging from 1 to 7 years of age were studied. A breath test was used to quantitate 13CO2 enrichment in exhaled air before and at 30, 60, and 120 min after the oral administration of 7 mg/kg of an aqueous solution of 1-13C-galactose to all children. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galactosemias; Isotope labeling; Children; Breath tests.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300280

Presence of t(14;18) translocation in healthy individuals varies according to ethnic background in the Brazilian population
Provedor de dados: 56
Autores: Levy,D.; Bertoldi,E.R.M.; Ruiz,J.L.M.; Pereira,J.; Bydlowski,S.P.
Several groups have demonstrated that healthy individuals can present the t(14;18) translocation. In this report, the presence of the translocation was examined in healthy blood donors in Brazil, a country considered an ethnic melting pot. The translocation was detected by nested PCR in 227 peripheral blood samples from individuals with different ethnic backgrounds. The t(14;18) translocation was found in 45 of 85 White individuals (52.94%); in 57 of 72 Black individuals (79.17%); and in 68 of 70 individuals (97.14%) of Japanese-descent. In conclusion, the frequency of the t(14;18) translocation in the Brazilian population varies according to the ethnic background.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T(14; 18) Lymphomas Characterization Molecular genetics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700602

Long-chain n-3 fatty acids and inflammation: potential application in surgical and trauma patients
Provedor de dados: 56
Autores: Calder,P.C.
Lipids used in nutritional support of surgical or critically ill patients have been based on soybean oil, which is rich in the n-6 fatty acid linoleic acid (18:2n-6). Linoleic acid is the precursor of arachidonic acid (20:4n-6). In turn, arachidonic acid in cell membrane phospholipids is the substrate for the synthesis of a range of biologically active compounds (eicosanoids) including prostaglandins, thromboxanes, and leukotrienes. These compounds can act as mediators in their own right and can also act as regulators of other processes, such as platelet aggregation, blood clotting, smooth muscle contraction, leukocyte chemotaxis, inflammatory cytokine production, and immune function. There is a view that an excess of n-6 fatty acids should be avoided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyunsaturated fatty acid; Fish oil; Inflammation; Eicosanoid; Cytokine; Critical illness.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400004

An experimental model for the measurement of renal function after temporary ureteral obstruction in the rat
Provedor de dados: 56
We present the results obtained with a ureterovesical implant after ipsilateral ureteral obstruction in the rat, suitable for the study of renal function after deobstruction in these animals. Thirty-seven male Wistar rats weighing 260 to 300 g were submitted to distal right ureteral ligation and divided into 3 groups, A (N = 13, 1 week of obstruction), B (N = 14, 2 weeks of obstruction) and C (N = 10, 3 weeks of obstruction). The animals were then submitted to ureterovesical implantation on the right side and nephrectomy on the left side. During the 4-week follow-up period serum levels of urea and creatinine were measured on the 2nd, 7th, 14th, 21st and 28th day and compared with preoperative levels. The ureterovesical implantation included a psoas hitch...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Kidney function; Rat; Ureteral obstruction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700006

Protein-energy malnutrition halts hemopoietic progenitor cells in the G0/G1 cell cycle stage, thereby altering cell production rates
Provedor de dados: 56
Autores: Borelli,P.; Barros,F.E.V.; Nakajima,K.; Blatt,S.L.; Beutler,B.; Pereira,J.; Tsujita,M.; Favero,G.M.; Fock,R.A.
Protein energy malnutrition (PEM) is a syndrome that often results in immunodeficiency coupled with pancytopenia. Hemopoietic tissue requires a high nutrient supply and the proliferation, differentiation and maturation of cells occur in a constant and balanced manner, sensitive to the demands of specific cell lineages and dependent on the stem cell population. In the present study, we evaluated the effect of PEM on some aspects of hemopoiesis, analyzing the cell cycle of bone marrow cells and the percentage of progenitor cells in the bone marrow. Two-month-old male Swiss mice (N = 7-9 per group) were submitted to PEM with a low-protein diet (4%) or were fed a control diet (20% protein) ad libitum. When the experimental group had lost about 20% of their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malnutrition; Hemopoiesis; Cell cycle; Low protein diet.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600008

Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone
Provedor de dados: 56
Autores: Fukumasu,H.; Cordeiro,Y.G.; Rochetti,A.L.; Barra,C.N.; Sámora,T.S.; Strefezzi,R.F.; Dagli,M.L.Z.
Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: NR1I3; CAR; NNK; Cyclin D1; Lung carcinogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300240

Effect of the human leukocyte antigen HLA-DRB1 and anti-cyclic citrullinated peptide on the outcome of rheumatoid arthritis patients
Provedor de dados: 56
Autores: Farouk,H.M.; Mansour,H.E.; Rahman,S.A.; Mostafa,A.A.; Shamy,H.A.; Zarouk,W.A.
Our objective was to determine whether the presence of the human leukocyte antigen HLA-DRB1 locus is associated with production of anti-cyclic citrullinated peptide antibodies (anti-CCP Abs) and to what extent they are associated with increased susceptibility to and severity of rheumatoid arthritis (RA) in Egyptian patients. Twenty-nine RA patients gave informed consent to participate in a case-control study that was approved by the Ain Shams University Medical Ethics Committee. RA disease activity and severity were determined using the simplified disease activity index and Larsen scores, respectively. We used a wide scale national study on the pattern of HLA typing in normal Egyptians as a control study. Anti-CCP Abs and HLA-DRB1 typing were determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-DRB1; Anti-CCP Ab; Rheumatoid arthritis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900010

Estrogen replacement therapy and cardioprotection: mechanisms and controversies
Provedor de dados: 56
Autores: Subbiah,M.T.R.
Epidemiological and case-controlled studies suggest that estrogen replacement therapy might be beneficial in terms of primary prevention of coronary heart disease (CHD). This beneficial effect of estrogens was initially considered to be due to the reduction of low density lipoproteins (LDL) and to increases in high density lipoproteins (HDL). Recent studies have shown that estrogens protect against oxidative stress and decrease LDL oxidation. Estrogens have direct effects on the arterial tissue and modulate vascular reactivity through nitric oxide and prostaglandin synthesis. While many of the effects of estrogen on vascular tissue are believed to be mediated by estrogen receptors alpha and ß, there is evidence for `immediate non-genomic' effects. The role...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogen therapy; Postmenopausal women; Cardiovascular disease; Plasma lipoproteins; Lipoprotein oxidation; Estrogen receptors; Thrombosis; Nitric oxide synthesis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300001

Physical exercise-induced fatigue: the role of serotonergic and dopaminergic systems
Provedor de dados: 56
Autores: Cordeiro,L.M.S.; Rabelo,P.C.R.; Moraes,M.M.; Teixeira-Coelho,F.; Coimbra,C.C.; Wanner,S.P.; Soares,D.D.
Brain serotonin and dopamine are neurotransmitters related to fatigue, a feeling that leads to reduced intensity or interruption of physical exercises, thereby regulating performance. The present review aims to present advances on the understanding of fatigue, which has recently been proposed as a defense mechanism instead of a “physiological failure” in the context of prolonged (aerobic) exercises. We also present recent advances on the association between serotonin, dopamine and fatigue. Experiments with rodents, which allow direct manipulation of brain serotonin and dopamine during exercise, clearly indicate that increased serotoninergic activity reduces performance, while increased dopaminergic activity is associated with increased performance....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Lethargy; Monoamines; Performance; Physical activity; Reward.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200301

The erythrocyte cytoskeleton protein 4.2 is not demonstrable in several mammalian species
Provedor de dados: 56
Autores: Guerra-Shinohara,E.M.; Barretto,O.C. de O.
Erythrocyte membrane proteins from 44 representative mammals were studied. Protein 4.2 was not detected in guinea pigs (Cavia porcellus) (N = 14), Southern Brazilian swamp large rats (Myocastor coypus) (N = 2), cutias (Dasyprocta sp) (N = 4), and horses (Equus caballus) (N = 13). These animals also presented high ankyrin concentrations except for the horse which did not exhibit a sharp band, although minor components located between proteins 2 and 3 could account for the ankyrin family. The rodents studied did present band 6, which was not detectable in other common rodents such as white rats (Rattus norvegicus) (N = 9) and mice (Mus musculus) (N = 12). Since the absence of protein 4.2 does not disrupt the cytoskeleton membrane, we suggest that it is not...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Protein 4.2; Erythrocyte membrane cytoskeleton; Mammals.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600003

Effect of the aerobic capacity on the validity of the anaerobic threshold for determination of the maximal lactate steady state in cycling
Provedor de dados: 56
Autores: Denadai,B.S.; Figueira,T.R.; Favaro,O.R.P.; Gonçalves,M.
The maximal lactate steady state (MLSS) is the highest blood lactate concentration that can be identified as maintaining a steady state during a prolonged submaximal constant workload. The objective of the present study was to analyze the influence of the aerobic capacity on the validity of anaerobic threshold (AT) to estimate the exercise intensity at MLSS (MLSS intensity) during cycling. Ten untrained males (UC) and 9 male endurance cyclists (EC) matched for age, weight and height performed one incremental maximal load test to determine AT and two to four 30-min constant submaximal load tests on a mechanically braked cycle ergometer to determine MLSS and MLSS intensity. AT was determined as the intensity corresponding to 3.5 mM blood lactate. MLSS...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anaerobic threshold; Lactate steady state; Aerobic capacity; Cycling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000015

Water deprivation and the double- depletion hypothesis: common neural mechanisms underlie thirst and salt appetite
Provedor de dados: 56
Autores: De Luca,L.A. Jr; Vendramini,R.C.; Pereira,D.T.B.; Colombari,D.A.S.; David,R.B.; Paula,P.M.; Menani,J.V.
Water deprivation-induced thirst is explained by the double-depletion hypothesis, which predicts that dehydration of the two major body fluid compartments, the extracellular and intracellular compartments, activates signals that combine centrally to induce water intake. However, sodium appetite is also elicited by water deprivation. In this brief review, we stress the importance of the water-depletion and partial extracellular fluid-repletion protocol which permits the distinction between sodium appetite and thirst. Consistent enhancement or a de novo production of sodium intake induced by deactivation of inhibitory nuclei (e.g., lateral parabrachial nucleus) or hormones (oxytocin, atrial natriuretic peptide), in water-deprived, extracellular-dehydrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water deprivation; Dehydration; Mineral preference; Sodium appetite; Cravings.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500015

The ERBB2 gene polymorphisms rs2643194, rs2934971, and rs1058808 are associated with increased risk of gastric cancer
Provedor de dados: 56
Autores: Vázquez-Ibarra,K.C.; Bustos-Carpinteyro,A.R.; García-Ruvalcaba,A.; Magaãa-Torres,M.T.; Gutiérrez-Aguilar,R.; Marín-Contreras,M.E.; Santiago-Luna,E.; Sánchez-López,J.Y.
Gastric cancer (GC) is the third most lethal type of cancer worldwide. Single nucleotide polymorphisms (SNPs) in regulatory sites or coding regions can modify the expression of genes involved in gastric carcinogenesis, as ERBB2, which encodes for the tyrosine-kinase receptor HER-2. The aim of this work was to analyze the association of the polymorphisms: rs2643194, rs2517951, rs2643195, rs2934971, and rs1058808 with GC, as they have not yet been analyzed in GC patients, as well as to report their frequency in the general Mexican population (GMP). We studied genomic DNA from subjects with GC (n=74), gastric inflammatory diseases (GID, n=76 control subjects), and GMP (n=102). Genotypes were obtained by means of real-time PCR and DNA-sequencing. The risks for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ERBB2 gene; Gastric cancer; HER2; Polymorphisms.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500611

Defense reaction induced by a metabotropic glutamate receptor agonist microinjected into the dorsal periaqueductal gray of rats
Provedor de dados: 56
Autores: Molchanov,M.L.; Guimarães,F.S.
The behavioral effects of trans-(±)-1-amino-1,3-cyclopentanedicarboxylic acid (t-ACPD), a metabotropic glutamate receptor (mGluR) agonist, or 0.9% (w/v) saline, injected into the dorsal periaqueductal gray (DPAG), was investigated. Male Wistar rats showed defense reactions characterized by jumps toward the top edges of the cages (saline = 0 vs t-ACPD = 6.0, medians P&amp;lt;0.05) and gallops (saline = 0 vs t-ACPD = 10.0, medians P&amp;lt;0.05) during the 60-s period after the beginning of the injection. In another experiment animals were placed inside an open arena for 5 min immediately after injection. Their behavior was recorded by a video camera and a computer program analyzed the videotapes. Eleven of fifteen rats injected with t-ACPD showed a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Periaqueductal gray; Metabotropic glutamate receptors; Defense behavior; T-ACPD; Open-field; Rats.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200013

High doses of riboflavin and the elimination of dietary red meat promote the recovery of some motor functions in Parkinson's disease patients
Provedor de dados: 56
Autores: Coimbra,C.G.; Junqueira,V.B.C.
Abnormal riboflavin status in the absence of a dietary deficiency was detected in 31 consecutive outpatients with Parkinson's disease (PD), while the classical determinants of homocysteine levels (B6, folic acid, and B12) were usually within normal limits. In contrast, only 3 of 10 consecutive outpatients with dementia without previous stroke had abnormal riboflavin status. The data for 12 patients who did not complete 6 months of therapy or did not comply with the proposed treatment paradigm were excluded from analysis. Nineteen PD patients (8 males and 11 females, mean age ± SD = 66.2 ± 8.6 years; 3, 3, 2, 5, and 6 patients in Hoehn and Yahr stages I to V) received riboflavin orally (30 mg every 8 h) plus their usual symptomatic medications and all red...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson's disease; Riboflavin; Flavin-adenine dinucleotide; Glutathione; Iron; Hemin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000019

Response of the arterial blood pressure of quadriplegic patients to treadmill gait training
Provedor de dados: 56
Autores: Carvalho,D.C.L.; Cliquet Jr.,A.
Blood pressure pattern was analyzed in 12 complete quadriplegics with chronic lesions after three months of treadmill gait training. Before training, blood pressure values were obtained at rest, during treadmill walking and during the recovery phase. Gait training was performed for 20 min twice a week for three months. Treadmill gait was achieved using neuromuscular electrical stimulation, assisted by partial body weight relief (30-50%). After training, blood pressure was evaluated at rest, during gait and during recovery phase. Before and after training, mean systolic blood pressures and heart rates increased significantly during gait compared to rest (94.16 ± 5.15 to 105 ± 5.22 mmHg and 74.27 ± 10.09 to 106.23 ± 17.31 bpm, respectively), and blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quadriplegia; Treadmill gait; Neuromuscular electrical stimulation; Body weight support; Blood pressure; Orthostatic hypotension.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900011

The neurobiology of infant maternal odor learning
Provedor de dados: 56
Autores: Raineki,C.; Pickenhagen,A.; Roth,T.L.; Babstock,D.M.; McLean,J.H.; Harley,C.W.; Lucion,A.B.; Sullivan,R.M.
Infant rats must learn to identify their mother’s diet-dependent odor. Once learned, maternal odor controls pups’ approach to the mother, their social behavior and nipple attachment. Here we present a review of the research from four different laboratories, which suggests that neural and behavioral responses to the natural maternal odor and neonatal learned odors are similar. Together, these data indicate that pups have a unique learning circuit relying on the olfactory bulb for neural plasticity and on the hyperfunctioning noradrenergic locus coeruleus flooding the olfactory bulb with norepinephrine to support the neural changes. Another important factor making this system unique is the inability of the amygdala to become incorporated into the infant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maternal odor; Olfactory bulb; Norepinephrine; Attachment; Locus coeruleus; Amygdala.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000001

Topical application of a melanotropin analogue to vulgar vitiligo dermo-epidermal minigrafts
Provedor de dados: 56
Autores: Schwartzmann-Solon,A.M.; Visconti,M.A.; Castrucci,A.M.L.
Human subjects with active vulgar vitiligo do not respond well to autologous dermo-epidermal minigrafting. Eighteen subjects were treated with the &lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-melanocyte-stimulating hormone (&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-MSH) synthetic analogue &amp;amp;#091;Nle4, D-Phe7&amp;amp;#093;-&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-MSH. The hormone (50 µl, 0.4 mM) was applied topically to 30-cm2 lesions in which 29-48 minigrafts had been made. The hormone did not improve the success of the minigrafting and no differences were observed in local or distant repigmentation in treated subjects as compared to the placebo group. Aliquots of 24-h urine concentrated by lyophilization irreversibly darkened toad skins, demonstrating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vulgar vitiligo &lt; FONT FACE=Symbol&gt; A&lt; /FONT&gt; -MSH synthetic analogue minigrafting.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200008

The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
Provedor de dados: 56
Autores: Borja-Oliveira,C.R.; Durigon,A.M.; Vallin,A.C.C.; Toyama,M.H.; Souccar,C.; Marangoni,S.; Rodrigues-Simioni,L.
The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chick biventer cervicis; Myotoxicity; Neurotoxicity; Phospholipase A2; Presynaptic action.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500009

Obesity inversely correlates with prostate-specific antigen levels in a population with normal screening results of prostate cancer in northwestern China
Provedor de dados: 56
Autores: Zhang,J.; Ma,M.; Nan,X.; Sheng,B.
Serum prostate-specific antigen (PSA) is a diagnostic biomarker of prostate cancer and is possibly associated with obesity. This study aimed to explore the relationships between obesity indicators [body mass index (BMI) and waist circumference (WC)] with PSA in Chinese men. A cross-sectional study of men aged 30-85 years undergoing prostate cancer screening was conducted from August 2008 to July 2013 in Xi'an, China. Data were obtained from clinical reports, condition was recorded based on self-report including demographics, weight, height, and WC (&gt;90 cm=obese). Fasting blood glucose (FBG) and prostate volume (PV) were assessed clinically. Patients were grouped by BMI (normal=22.9, overweight=23-27.4, obese≥27.5 kg/m2). PSA parameters of density...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate-specific antigen; Body mass index; Obesity; Prostate cancer; Screening.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800704

Single-step purification of crotapotin and crotactine from Crotalus durissus terrificus venom using preparative isoelectric focusing
Provedor de dados: 56
We describe the isolation of crotoxin, a presynaptic B-neurotoxin, as well as its subunits B (crotactine) and A (crotapotin) from lyophilized Crotalus durissus terrificus venom by a single-step preparative isoelectric focusing procedure. From 98 mg of dried venom protein 20.1 mg of crotactine and 13.1 mg of crotapotin were recovered in the first step of focalization and 4.2 mg in a second run. These values correspond to 35.7% of the total venom protein applied. Crotactine separated in the 9.3-7.0 pH range (tubes 1-6) and crotapotin in the 1.8-2.8 pH range (tubes 15-19) and both were homogeneous by SDS-PAGE and N-terminal amino acid analysis. Crotactine, a 12-kDa protein, presented hemolytic and phospholipase A2 activity. Thus, using isoelectric focusing we...
Palavras-chave: Crotalus durissus terrificus; Crotactine; Crotapotin; Phospholipase A2; Isoelectrofocusing; Presynaptic B-neurotoxin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100004

Immunophenotype of hematopoietic stem cells from placental/umbilical cord blood after culture
Provedor de dados: 56
Autores: Pranke,P.; Hendrikx,J.; Debnath,G.; Alespeiti,G.; Rubinstein,P.; Nardi,N.; Visser,J.
Identification and enumeration of human hematopoietic stem cells remain problematic, since in vitro and in vivo stem cell assays have different outcomes. We determined if the altered expression of adhesion molecules during stem cell expansion could be a reason for the discrepancy. CD34+CD38- and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin (TPO), FLT-3 ligand (FL) and kit ligand (KL; or stem cell factor) in different combinations: TPO + FL + KL, TPO + FL and TPO, at concentrations of 50 ng/mL each. Cells were immunophenotyped by four-color fluorescence using antibodies against CD11c, CD31, CD49e, CD61, CD62L, CD117, and HLA-DR. Low-density cord blood contained 1.4 ± 0.9% CD34+ cells, 2.6 ± 2.1% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoietic stem cells; CD34+CD38- cells; Human umbilical cord blood; Ex vivo expansion; Adhesion molecules.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200006

Mesenteric lymph reperfusion exacerbates spleen injury caused by superior mesenteric artery occlusion shock
Provedor de dados: 56
Autores: Li,L.L.; Zhang,C.H.; Liu,J.C.; Yang,L.N.; Niu,C.Y.; Zhao,Z.G.
The intestinal lymph pathway plays an important role in the pathogenesis of organ injury following superior mesenteric artery occlusion (SMAO) shock. We hypothesized that mesenteric lymph reperfusion (MLR) is a major cause of spleen injury after SMAO shock. To test this hypothesis, SMAO shock was induced in Wistar rats by clamping the superior mesenteric artery (SMA) for 1 h, followed by reperfusion for 2 h. Similarly, MLR was performed by clamping the mesenteric lymph duct (MLD) for 1 h, followed by reperfusion for 2 h. In the MLR+SMAO group rats, both the SMA and MLD were clamped and then released for reperfusion for 2 h. SMAO shock alone elicited: 1) splenic structure injury, 2) increased levels of malondialdehyde, nitric oxide (NO), intercellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenteric lymph reperfusion; Superior mesenteric artery; Shock; Spleen injury; Endotoxin; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500376

A prospective study of hepatitis B virus markers in patients with chronic HBV infection from Brazilian families of Western and Asian origin
Provedor de dados: 56
Autores: Carrilho,F.J.; Ono-Nita,S.K.; Cardoso,R.A.; Cancado,E.L.R.; Pinho,J.R.R.; Alves,V.A.F.; Da Silva,L.C.
The purpose of the present study was to determine the frequency of hepatitis B virus (HBV) markers in families of HBsAg-positive patients with chronic liver disease. Serum anti-HBc, HBsAg and anti-HBs were determined by enzyme immunoassay and four subpopulations were considered: genetically related (consanguineous) and non-genetically related (non-consanguineous) Asian subjects and genetically related and non-genetically related Western subjects. A total of 165 and 186 relatives of Asian and Western origin were enrolled, respectively. The occurrence of HBsAg and anti-HBs antibodies was significantly higher (P &lt; 0.0001) in family members of Asian origin (81.8%) than in family members of Western origin (36.5%). HBsAg was also more frequent among brothers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B; Familial clustering; HBsAg; Vertical transmission; Anti-HBc; Anti-HBs.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900015

Dimensionality of the premenstrual syndrome: confirmatory factor analysis of premenstrual dysphoric symptoms among college students
Provedor de dados: 56
Autores: Wang,Y.-P.; Teng,C.-T.; Vieira Filho,A.H.G.; Gorenstein,C.; Andrade,L.H.
Premenstrual syndrome and premenstrual dysphoric disorder (PMDD) seem to form a severity continuum with no clear-cut boundary. However, since the American Psychiatric Association proposed the research criteria for PMDD in 1994, there has been no agreement about the symptomatic constellation that constitutes this syndrome. The objective of the present study was to establish the core latent structure of PMDD symptoms in a non-clinical sample. Data concerning PMDD symptoms were obtained from 632 regularly menstruating college students (mean age 24.4 years, SD 5.9, range 17 to 49). For the first random half (N = 316), we performed principal component analysis (PCA) and for the remaining half (N = 316), we tested three theory-derived competing models of PMDD by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premenstrual dysphoric disorder; Premenstrual syndrome; Dysphoria; Factor analysis; Psychometric properties.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500006

Effect of policosanol on cerebral ischemia in Mongolian gerbils
Provedor de dados: 56
Autores: Molina,V.; Arruzazabala,M.L.; Carbajal,D.; Valdés,S.; Noa,M.; Más,R.; Fraga,V.; Menéndez,R.
Policosanol is a mixture of higher aliphatic primary alcohols isolated from sugar cane wax, whose main component is octacosanol. An inhibitory effect of policosanol on platelet aggregation and cerebral ischemia in animal models has been reported. Thus, the objective of the present study was to evaluate the effect of policosanol on cerebral ischemia induced by unilateral carotid ligation and bilateral clamping and recirculation in Mongolian gerbils. Policosanol (200 mg/kg) administered immediately after unilateral carotid ligation and at 12- or 24-h intervals for 48 h significantly inhibited mortality and clinical symptoms when compared with controls, whereas lower doses (100 mg/kg) were not effective. Control animals showed swelling (tissue vacuolization)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia; Gerbils; Policosanol; Reperfusion brain edema.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000014

Prevalence of BRCA1 and BRCA2 gene mutations in families with medium and high risk of breast and ovarian cancer in Brazil
Provedor de dados: 56
Autores: Esteves,V.F.; Thuler,L.C.S.; Amêndola,L.C.; Koifman,R.J.; Koifman,S.; Frankel,P.P.; Vieira,R.J.S.
Of all malignant neoplasias affecting women, breast cancer has the highest incidence rate in Brazil. The objective of the present study was to determine the frequency of genetic modifications in families with medium and high risk for breast and ovarian cancer from different regions of Brazil. An exploratory, descriptive study was carried out on the prevalence of the BRCA1 and BRCA2 mutations in case series of high-risk families for breast and/or ovarian cancer. After heredogram construction, a blood sample was taken and DNA extraction was performed in all index cases. The protein truncation test was used to screen for truncated mutations in exon 11 of the BRCA1 gene and in exons 10 and 11 of the BRCA2 gene. Of the 612 individuals submitted to genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; BRCA1; BRCA2; Mutations; Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500009

Low-level red laser therapy alters effects of ultraviolet C radiation on Escherichia coli cells
Provedor de dados: 56
Autores: Canuto,K.S.; Sergio,L.P.S.; Guimarães,O.R.; Geller,M.; Paoli,F.; Fonseca,A.S.
Low-level lasers are used at low power densities and doses according to clinical protocols supplied with laser devices or based on professional practice. Although use of these lasers is increasing in many countries, the molecular mechanisms involved in effects of low-level lasers, mainly on DNA, are controversial. In this study, we evaluated the effects of low-level red lasers on survival, filamentation, and morphology of Escherichia colicells that were exposed to ultraviolet C (UVC) radiation. Exponential and stationary wild-type and uvrA-deficient E. coli cells were exposed to a low-level red laser and in sequence to UVC radiation. Bacterial survival was evaluated to determine the laser protection factor (ratio between the number of viable cells after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Escherichia coli; Laser; Ultraviolet radiation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000012

Association of polymorphisms at the ADIPOR1 regulatory region with type 2 diabetes and body mass index in a Brazilian population with European or African ancestry
Provedor de dados: 56
Autores: Yeh,E.; Kimura,L.; Errera,F.I.V.; Angeli,C.B.; Mingroni-Netto,R.C.; Silva,M.E.R.; Canani,L.H.S.; Passos-Bueno,M.R.
Association studies between ADIPOR1 genetic variants and predisposition to type 2 diabetes (DM2) have provided contradictory results. We determined if two single nucleotide polymorphisms (SNP c.-8503G&gt;A and SNP c.10225C&gt;G) in regulatory regions of ADIPOR1 in 567 Brazilian individuals of European (EA; N = 443) or African (AfA; N = 124) ancestry from rural (quilombo remnants; N = 439) and urban (N = 567) areas. We detected a significant effect of ethnicity on the distribution of the allelic frequencies of both SNPs in these populations (EA: -8503A = 0.27; AfA: -8503A = 0.16; P = 0.001 and EA: 10225G = 0.35; AfA: 10225G = 0.51; P &lt; 0.001). Neither of the polymorphisms were associated with DM2 in the case-control study in EA (SNP c.-8503G&gt;A: DM2...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Association study; Adiponectin receptors; Type 2 diabetes; Polymorphism for ancestry-admixture mapping; HDL cholesterol; Association of ADIPOR1 with DM2.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600005

Trends in adjuvant development for vaccines: DAMPs and PAMPs as potential new adjuvants
Provedor de dados: 56
Autores: Miyaji,E.N.; Carvalho,E.; Oliveira,M.L.S.; Raw,I.; Ho,P.L.
Aluminum salts have been widely used in vaccine formulations and, after their introduction more than 80 years ago, only few vaccine formulations using new adjuvants were developed in the last two decades. Recent advances in the understanding of how innate mechanisms influence the adaptive immunity opened up the possibility for the development of new adjuvants in a more rational design. The purpose of this review is to discuss the recent advances in this field regarding the attempts to determine the molecular basis and the general mechanisms underlying the development of new adjuvants, with particular emphasis on the activation of receptors of innate immune recognition. One can anticipate that the use of these novel adjuvants will also provide a window of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adjuvant; Vaccine; Innate immunity; DAMP; PAMP.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600003

Heparan sulfate and cell division
Provedor de dados: 56
Autores: Porcionatto,M.A.; Nader,H.B.; Dietrich,C.P.
Heparan sulfate is a component of vertebrate and invertebrate tissues which appears during the cytodifferentiation stage of embryonic development. Its structure varies according to the tissue and species of origin and is modified during neoplastic transformation. Several lines of experimental evidence suggest that heparan sulfate plays a role in cellular recognition, cellular adhesion and growth control. Heparan sulfate can participate in the process of cell division in two distinct ways, either as a positive or negative modulator of cellular proliferation, or as a response to a mitogenic stimulus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparan sulfate; Glycosaminoglycan; Proteoglycan; Cell division; Cell cycle; Growth factors.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500006

There are more asthmatics in homes with high cockroach infestation
Provedor de dados: 56
Autores: Sarinho,E.; Schor,D.; Veloso,M.A.; Rizzo,J.A.
Although asthma has been commonly associated with sensitivity to cockroaches, a clear causal relationship between asthma, allergy to cockroaches and exposure levels has not been extensively investigated. The objective of the present study was to determine whether asthma occurs more frequently in children living in homes with high cockroach infestation. The intensity of household infestation was assessed by the number of dead insects after professional pesticide application. Children living in these houses in the metropolitan area of Recife, PE, were diagnosed as having asthma by means of a questionnaire based on the ISAAC study. All children had physician-diagnosed asthma and at least one acute exacerbation in the past year. Children of both sexes aged 4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cockroaches; Asthma; Atopy; Public health; Plague control.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400007

Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents)
Provedor de dados: 56
Autores: Calixto,J.B.
This review highlights the current advances in knowledge about the safety, efficacy, quality control, marketing and regulatory aspects of botanical medicines. Phytotherapeutic agents are standardized herbal preparations consisting of complex mixtures of one or more plants which contain as active ingredients plant parts or plant material in the crude or processed state. A marked growth in the worldwide phytotherapeutic market has occurred over the last 15 years. For the European and USA markets alone, this will reach about $7 billion and $5 billion per annum, respectively, in 1999, and has thus attracted the interest of most large pharmaceutical companies. Insufficient data exist for most plants to guarantee their quality, efficacy and safety. The idea that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbal medicine; Efficacy; Safety; Quality control; Market; Regulatory guidelines.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200004

Duodenal-jejunal bypass normalizes pancreatic islet proliferation rate and function but not hepatic steatosis in hypothalamic obese rats
Provedor de dados: 56
Autores: Cantelli,K.R.; Soares,G.M.; Ribeiro,R.A.; Balbo,S.L.; Lubaczeuski,C.; Boschero,A.C.; Araújo,A.C.F.; Bonfleur,M.L.
Modifications in life-style and/or pharmacotherapies contribute to weight loss and ameliorate the metabolic profile of diet-induced obese humans and rodents. Since these strategies fail to treat hypothalamic obesity, we have assessed the possible mechanisms by which duodenal-jejunal bypass (DJB) surgery regulates hepatic lipid metabolism and the morphophysiology of pancreatic islets, in hypothalamic obese (HyO) rats. During the first 5 days of life, male Wistar rats received subcutaneous injections of monosodium glutamate (4 g/kg body weight, HyO group), or saline (CTL). At 90 days of age, HyO rats were randomly subjected to DJB (HyO DJB group) or sham surgery (HyO Sham group). HyO Sham rats were morbidly obese, insulin resistant, hypertriglyceridemic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duodenal-jejunal bypass; Hepatic fatty acid metabolism; Hypothalamic obesity; Ki67; Insulin secretion.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500601

Bone mineral density in young women of the city of São Paulo, Brazil: correlation with both collagen type I alpha 1 gene polymorphism and clinical aspects
Provedor de dados: 56
Autores: Barros,E.R.; Kasamatsu,T.S.; Ramalho,A.C.; Hauache,O.M.; Vieira,J.G.H.; Lazaretti-Castro,M.
Osteoporosis is a multifactorial disease with great impact on morbidity and mortality mainly in postmenopausal women. Although it is recognized that factors related to life-style and habits may influence bone mass formation leading to greater or lower bone mass, more than 85% of the variation in bone mineral density (BMD) is genetically determined. The collagen type I alpha 1 (COLIA1) gene is a possible risk factor for osteoporosis. We studied a population of 220 young women from the city of São Paulo, Brazil, with respect to BMD and its correlation with both COLIA1 genotype and clinical aspects. The distribution of COLIA1 genotype SS, Ss and ss in the population studied was 73.6, 24.1 and 2.3%, respectively. No association between these genotypes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COLIA1; Bone mineral density; Risk factors; Polymorphism; Osteoporosis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800005

Cannabidiol, a Cannabis sativa constituent, as an antipsychotic drug
Provedor de dados: 56
Autores: Zuardi,A.W.; Crippa,J.A.S.; Hallak,J.E.C.; Moreira,F.A.; Guimarães,F.S.
A high dose of delta9-tetrahydrocannabinol, the main Cannabis sativa (cannabis) component, induces anxiety and psychotic-like symptoms in healthy volunteers. These effects of delta9-tetrahydrocannabinol are significantly reduced by cannabidiol (CBD), a cannabis constituent which is devoid of the typical effects of the plant. This observation led us to suspect that CBD could have anxiolytic and/or antipsychotic actions. Studies in animal models and in healthy volunteers clearly suggest an anxiolytic-like effect of CBD. The antipsychotic-like properties of CBD have been investigated in animal models using behavioral and neurochemical techniques which suggested that CBD has a pharmacological profile similar to that of atypical antipsychotic drugs. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cannabidiol; D9-Tetrahydrocannabinol; Cannabinoid; Anxiety; Antipsychotic; Schizophrenia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400001

Time course of changes in heart rate and blood pressure variability in streptozotocin-induced diabetic rats treated with insulin
Provedor de dados: 56
Autores: Schaan,B.D.; Maeda,C.Y.; Timm,H.B.; Medeiros,S.; Moraes,R.S.; Ferlin,E.; Fernandes,T.G.; Ribeiro,J.P.; Schmid,H.; Irigoyen,M.C.
Autonomic neuropathy is a frequent complication of diabetes associated with higher morbidity and mortality in symptomatic patients, possibly because it affects autonomic regulation of the sinus node, reducing heart rate (HR) variability which predisposes to fatal arrhythmias. We evaluated the time course of arterial pressure and HR and indirectly of autonomic function (by evaluation of mean arterial pressure (MAP) variability) in rats (164.5 ± 1.7 g) 7, 14, 30 and 120 days after streptozotocin (STZ) injection, treated with insulin, using measurements of arterial pressure, HR and MAP variability. HR variability was evaluated by the standard deviation of RR intervals (SDNN) and root mean square of successive difference of RR intervals (RMSSD). MAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Streptozotocin; Insulin; Autonomic nervous system.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900006

Immunopathology of the duodenal mucosa of HIV-positive patients during combined antiretroviral therapy
Provedor de dados: 56
Autores: Machado,F.R.; Pagliari,C.; Caiafa,H.; Tapajós,R.; Duarte,M.I.S.
The objective of the present study was to evaluate the duodenal mucosa of HIV-infected patients during antiretroviral therapy. This was an observational study conducted on HIV-positive patients and a control group. Group 1 comprised 22 HIV-negative individuals while 38 HIV-positive individuals were classified according to the CDC 1993 classification into group 2 (A1 or A2) or group 3 (B2, A3, B3, C2, C3). All subjects were submitted to upper gastrointestinal endoscopy with duodenal biopsies. Qualitative, semi-quantitative and quantitative histological analyses were performed. Results were considered significant when P &lt; 0.05. A higher prevalence of inflammatory infiltrate and eosinophilia was observed in the HIV group, together with a reduction in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal histopathology; Cytokines; Human immunodeficiency virus; Acquired immunodeficiency syndrome; Antiretroviral therapy; CD4+.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100013

Prevalence of low trauma fractures in long-term kidney transplant patients with preserved renal function
Provedor de dados: 56
Autores: Braga Júnior,J.W.R.; Neves,R.M.S.; Pinheiro,M.M.; Frisoli Júnior,A.; Castro,C.H.M.; Szejnfeld,V.L.; Carvalho,A.B.
We evaluated the prevalence of low bone mineral density (BMD) and osteoporotic fractures in kidney transplantation (KT) patients and determined risk factors associated with osteoporotic fractures. The study was conducted on 191 patients (94 men and 97 women) with first KT for 3 years or more presenting stable and preserved renal function (serum creatinine levels lower than 2.5 mg/dl). KT patients were on immunosuppressive therapy and the cumulative doses of these drugs were also evaluated. BMD was determined by dual-energy X-ray absorptiometry at multiple sites (spine, femur and total body). Quantitative ultrasound of the calcaneus (broadband ultrasound attenuation, speed of sound, and stiffness index, SI) was also performed. Twenty-four percent (46) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney transplantation; Bone density; Quantitative ultrasound; Osteoporosis; Fracture.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100016

The mutagenic and antimutagenic effects of the traditional phytoestrogen-rich herbs, Pueraria mirifica and Pueraria lobata
Provedor de dados: 56
Autores: Cherdshewasart,W.; Sutjit,W.; Pulcharoen,K.; Chulasiri,M.
Pueraria mirifica is a Thai phytoestrogen-rich herb traditionally used for the treatment of menopausal symptoms. Pueraria lobata is also a phytoestrogen-rich herb traditionally used in Japan, Korea and China for the treatment of hypertension and alcoholism. We evaluated the mutagenic and antimutagenic activity of the two plant extracts using the Ames test preincubation method plus or minus the rat liver mixture S9 for metabolic activation using Salmonella typhimurium strains TA98 and TA100 as indicator strains. The cytotoxicity of the two extracts to the two S. typhimurium indicators was evaluated before the mutagenic and antimutagenic tests. Both extracts at a final concentration of 2.5, 5, 10, or 20 mg/plate exhibited only mild cytotoxic effects. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytoestrogen; Pueraria mirifica; Pueraria lobata; Mutagenic test; Antimutagenic test; Micronucleus test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900008

Yeast CUP1 protects HeLa cells against copper-induced stress
Provedor de dados: 56
Autores: Xie,X.X.; Ma,Y.F.; Wang,Q.S.; Chen,Z.L.; Liao,R.R.; Pan,Y.C.
As an essential trace element, copper can be toxic in mammalian cells when present in excess. Metallothioneins (MTs) are small, cysteine-rich proteins that avidly bind copper and thus play an important role in detoxification. Yeast CUP1 is a member of the MT gene family. The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress. In this study, CUP1 expression was determined by quantitative real-time PCR, and copper content was detected by inductively coupled plasma mass spectrometry. Production of intracellular reactive oxygen species (ROS) was evaluated using the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) assay. Cellular viability was detected using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Overexpression; Copper stress; Viability; ROS.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700616

Scientist-friendly policies for non-native English-speaking authors: timely and welcome
Provedor de dados: 56
Autores: Vasconcelos,S.M.R.; Sorenson,M.M.; Leta,J.
That English is the lingua franca of today's science is an indisputable fact. Publication in English in international journals is a pre-requisite for a research paper to gain visibility in academia. However, English proficiency appears to be taken for granted in the scientific community, though this language can be a hurdle for a number of authors, particularly from non-native English-speaking countries. The influence of English proficiency on the publication output of Brazilian authors has never been assessed. We report our preliminary data on the relationship between the English proficiency of 51,223 researchers registered in the CNPq database and their publication output in international journals. We have found that publication rates are higher for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Science policy; Research output; English proficiency; International journals; Scientific writing; Education.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600001

Brazilian version of the Berg balance scale
Provedor de dados: 56
Autores: Miyamoto,S.T.; Lombardi Junior,I.; Berg,K.O.; Ramos,L.R.; Natour,J.
The purpose of the present study was to translate and adapt the Berg balance scale, an instrument for functional balance assessment, to Brazilian-Portuguese and to determine the reliability of scores obtained with the Brazilian adaptation. Two persons proficient in English independently translated the original scale into Brazilian-Portuguese and a consensus version was generated. Two translators performed a back translation. Discrepancies were discussed and solved by a panel. Forty patients older than 65 years and 40 therapists were included in the cultural adaptation phase. If more than 15% of therapists or patients reported difficulty in understanding an item, that item was reformulated and reapplied. The final Brazilian version was then tested on 36...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Balance; Outcome assessment; Disability evaluation; Rehabilitation; Geriatric patients; Postural control.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900017

Desmin: molecular interactions and putative functions of the muscle intermediate filament protein
Provedor de dados: 56
Autores: Costa,M.L.; Escaleira,R.; Cataldo,A.; Oliveira,F.; Mermelstein,C.S.
Desmin is the intermediate filament (IF) protein occurring exclusively in muscle and endothelial cells. There are other IF proteins in muscle such as nestin, peripherin, and vimentin, besides the ubiquitous lamins, but they are not unique to muscle. Desmin was purified in 1977, the desmin gene was characterized in 1989, and knock-out animals were generated in 1996. Several isoforms have been described. Desmin IFs are present throughout smooth, cardiac and skeletal muscle cells, but can be more concentrated in some particular structures, such as dense bodies, around the nuclei, around the Z-line or in costameres. Desmin is up-regulated in muscle-derived cellular adaptations, including conductive fibers in the heart, electric organs, some myopathies, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desmin; Intermediate filaments; Muscle; Myogenesis; Myopathy.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200007

Study of urinary acidification in patients with idiopathic hypocitraturia
Provedor de dados: 56
Autores: Araújo,N.C.; Rebelo,M.A.P.
Hypocitraturia (HCit) is one of the most remarkable features of renal tubular acidosis, but an acidification defect is not seen in the majority of hypocitraturic patients, whose disease is denoted idiopathic hypocitraturia. In order to assess the integrity of urinary acidification mechanisms in hypocitraturic idiopathic calcium stone formers, we studied two groups of patients, hypocitraturic (HCit, N = 21, 39.5 ± 11.5 years, 11 females and 10 males) and normocitraturic (NCit, N = 23, 40.2 ± 11.7 years, 16 females and 7 males) subjects, during a short ammonium chloride loading test lasting 8 h. During the baseline period HCit patients showed significantly higher levels of titratable acid (TA). After the administration of ammonium chloride, mean urinary pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary acidification; Hypocitraturia; Titratable acidity; Ammonium; Ammonium chloride load test; Lithiasis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200007

Genotyping of group A rotavirus samples from Brazilian children by probe hybridization
Provedor de dados: 56
Autores: Cardoso,D.D.P.; Rácz,M.L.; Azevedo,M.S.P.; Martins,R.M.B.; Soares,C.M.A.
The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. The knowledge of genotypes provides important information concerning rotavirus circulation in Central Brazil.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rotavirus; G genotyping; VP7; Oligonucleotides.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400005

Insulin impairs the maturation of chondrocytes in vitro
Provedor de dados: 56
Autores: Torres,E.S.; Andrade,C.V.; Fonseca,E.C.; Mello,M.A.; Duarte,M.E.L.
The precise nature of hormones and growth factors directly responsible for cartilage maturation is still largely unclear. Since longitudinal bone growth occurs through endochondral bone formation, excess or deficiency of most hormones and growth factors strongly influences final adult height. The structure and composition of the cartilaginous extracellular matrix have a critical role in regulating the behavior of growth plate chondrocytes. Therefore, the maintenance of the three-dimensional cell-matrix interaction is necessary to study the influence of individual signaling molecules on chondrogenesis, cartilage maturation and calcification. To investigate the effects of insulin on both proliferation and induction of hypertrophy in chondrocytes in vitro we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrocytes; Insulin; Micromass cultures; Apoptosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900007

Facilitatory effects of an auditory warning stimulus in a visual location identification task and a visual shape identification task
Provedor de dados: 56
Autores: Bueno,V.F.; Ribeiro-do-Valle,L.E.
The occurrence of a weak auditory warning stimulus increases the speed of the response to a subsequent visual target stimulus that must be identified. This facilitatory effect has been attributed to the temporal expectancy automatically induced by the warning stimulus. It has not been determined whether this results from a modulation of the stimulus identification process, the response selection process or both. The present study examined these possibilities. A group of 12 young adults performed a reaction time location identification task and another group of 12 young adults performed a reaction time shape identification task. A visual target stimulus was presented 1850 to 2350 ms plus a fixed interval (50, 100, 200, 400, 800, or 1600 ms, depending on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expectancy; Time course; Priming; Auditory temporal processing; Visual form processing; Reaction time.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100007

Hemodialysis improves endothelial venous function in end-stage renal disease
Provedor de dados: 56
Autores: Silva,A.M.V.; Signori,L.U.; Plentz,R.D.M.; Moreno Jr.,H.; Barros,E.; Belló-Klein,A.; Schaan,B.D.; Irigoyen,M.C.
The objective of the present study was to determine the acute effect of hemodialysis on endothelial venous function and oxidative stress. We studied 9 patients with end-stage renal disease (ESRD), 36.8 ± 3.0 years old, arterial pressure 133.8 ± 6.8/80.0 ± 5.0 mmHg, time on dialysis 55.0 ± 16.6 months, immediately before and after a hemodialysis session, and 10 healthy controls matched for age and gender. Endothelial function was assessed by the dorsal hand vein technique using graded local infusion of acetylcholine (endothelium-dependent venodilation, EDV) and sodium nitroprusside (endothelium-independent venodilation). Oxidative stress was evaluated by measuring protein oxidative damage (carbonyls) and antioxidant defense (total radical trapping...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: End-stage renal disease; Hemodialysis; Oxidative stress; Vascular endothelium.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600008

Retinal protective effects of topically administered agmatine on ischemic ocular injury caused by transient occlusion of the ophthalmic artery
Provedor de dados: 56
Autores: Hong,S.; Hara,H.; Shimazawa,M.; Hyakkoku,K.; Kim,C.Y.; Seong,G.J.
Agmatine, an endogenous polyamine and putative neuromodulator, is known to have neuroprotective effects on various neurons in the central nervous system. We determined whether or not topically administered agmatine could reduce ischemic retinal injury. Transient ocular ischemia was achieved by intraluminal occlusion of the middle cerebral artery of ddY mice (30-35 g) for 2 h, which is known to also induce occlusion of the ophthalmic artery. In the agmatine group (N = 6), a 1.0 mM agmatine-containing ophthalmic solution was administered four times daily for 2 weeks before occlusion. In the control group (N = 6), a 0.1% hyaluronic acid ophthalmic solution was instilled at the same times. At 22 h after reperfusion, the eyeballs were enucleated and the retinal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Agmatine; Apoptosis; Ischemia; Neuroprotection; Retina.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300005

Low prevalence of relevant findings in inappropriate echocardiograms and discordant perceptions between cardiologists and patients
Provedor de dados: 56
Autores: Lopes,J.R.; Oliveira,A.C.; Rios,V.G.; Correia,L.C.L.
Complementary examinations are “inadequate” whenever the likelihood of benefits from their indication is lower than the negative results. The low benefit is a result of poor performance in detecting relevant changes that lead to improved behavior. However, inadequate examinations are prevalent and little is known about patients' notions of the usefulness of such indications. The aim of this study was to describe relevant findings in inappropriate echocardiograms and to assess the level of agreement between patients and cardiologists regarding their usefulness. Adults without known cardiovascular disease who were referred for echocardiogram by inappropriate criteria according to the American College of Cardiology were selected. Relevant findings were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echocardiogram; Inappropriate; Utility; Patients; Cardiologists.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800606

Chemotherapy versus support cancer treatment in advanced gastric cancer: a meta-analysis
Provedor de dados: 56
Autores: Casaretto,L.; Sousa,P.L.R.; Mari,J.J.
The aim of the present study was to compare the efficacy of chemotherapy and support treatment in patients with advanced non-resectable gastric cancer in a systematic review and meta-analysis of randomized clinical trials that included a comparison of chemotherapy and support care treatment in patients diagnosed with gastric adenocarcinoma, regardless of their age, gender or place of treatment. The search strategy was based on the criteria of the Cochrane Base, using the following key words: 1) randomized clinical trials and antineoplastic combined therapy or gastrointestinal neoplasm, 2) stomach neoplasm and drug therapy, 3) clinical trial and multi-modality therapy, 4) stomach neoplasm and drug therapy or quality of life, 5) double-blind method or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric cancer; Systematic review; Combined antineoplastic therapy; Cancer support treatment; Meta-analysis; Chemotherapy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400002

Novel donors of nitric oxide derived of S-nitrosocysteine possessing antioxidant activities
Provedor de dados: 56
Autores: Petit,C.; Bernardes-Genisson,V.; Hoffmann,P.; Souchard,J.-P.; Labidalle,S.
Novel S-nitrosothiols possessing a phenolic function were investigated as nitric oxide (NO) donors. A study of NO release from these derivatives was carried out by electron spin resonance (ESR). All compounds gave rise to a characteristic three-line ESR signal in the presence of the complex [Fe(II)(MGD)2], revealing the formation of the complex [Fe(II)(MGD)2(NO)]. Furthermore, tests based on cytochrome c reduction were performed in order to study the ability of each phenolic disulfide, the final organic decomposition product of S-nitrosothiols, to trap superoxide radical anion (O2-). This study revealed a high reactivity of 1b and 3b towards O2-. For these two compounds, the respective inhibitory concentration (IC) 50 values were 92 µM and 43 µM.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; S-nitrosothiols (thionitrites); Antioxidant; Peroxynitrite.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100011

Somatic cytogenetic and azoospermia factor gene microdeletion studies in infertile men
Provedor de dados: 56
Autores: Pina-Neto,J.M.; Carrara,R.C.V.; Bisinella,R.; Mazzucatto,L.F.; Martins,M.D.; Sartoratto,E.; Yamasaki,R.
The objective of the present study was to determine the frequency of somatic chromosomal anomalies and Y chromosomal microdeletions (azoospermia factor genes, AZF) in infertile males who seek assisted reproduction. These studies are very important because the assisted reproduction techniques (mainly intracytoplasmic sperm injection) bypass the natural selection process and some classical chromosomal abnormalities, microdeletions of AZF genes or some deleterious genic mutations could pass through generations. These genetic abnormalities can cause in the offspring of these patients male infertility, ambiguous external genitalia, mental retardation, and other birth defects. We studied 165 infertile men whose infertility was attributable to testicular problems...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Y microdeletions; Male infertility; Karyotype; Chromosome aberrations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400017

Intrinsic bent DNA sites in the chromosomal replication origin of Xylella fastidiosa 9a5c
Provedor de dados: 56
Autores: Gimenes,F.; Gouveia,F. de S.; Fiorini,A.; Fernandez,M.A.
The features of the nucleotide sequences in both replication and promoter regions have been investigated in many organisms. Intrinsically bent DNA sites associated with transcription have been described in several prokaryotic organisms. The aim of the present study was to investigate intrinsic bent DNA sites in the segment that holds the chromosomal replication origin, oriC, of Xylella fastidiosa 9a5c. Electrophoretic behavior analyses, as well as in silico analyses of both the 2-D projection and helical parameters, were performed. The chromosomal segment analyzed contains the initial sequence of the rpmH gene, an intergenic region, the dnaA gene, the oriC sequence, and the 5' partial sequence of the dnaN gene. The analysis revealed fragments with reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intrinsic bent DNA; Prokaryotic replication origin; OriC; Xylella fastidiosa.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400007

Effect of the acupoints ST-36 (Zusanli) and SP-6 (Sanyinjiao) on intestinal myoelectric activity of Wistar rats
Provedor de dados: 56
Autores: Tabosa,A.; Yamamura,Y.; Forno,E.R.; Mello,L.E.A.M.
Despite its ancient use as a therapeutic tool to treat several ailments, acupuncture still faces the challenge of scrutiny by Western science both in terms of its efficacy and in terms of the characterization of its effects and mechanisms of actions underlying these effects. We investigated under well-controlled and carefully characterized conditions the influence of electrical stimulation of acupuncture points ST-36 (Zusanli) and SP-6 (Sanyinjiao) on the myoelectric activity of the small intestine of 38 adult male Wistar rats. Electrical recordings obtained by means of four electrodes chronically implanted in the small intestine were used to assess the effects of acupuncture (electroacupuncture stimulation set at 2 Hz, intermittent stimulation, 1 V, for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acupuncture; Intestinal motility; Rat; Myoelectric activity; Acupuncture point.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600015

Acute electrophysiologic consequences of pyridostigmine inhibition of cholinesterase in humans
Provedor de dados: 56
Autores: Zimerman,L.I.; Liberman,A.; Castro,R.R.T.; Ribeiro,J.P.; Nóbrega,A.C.L.
The cardiovascular electrophysiologic basis for the action of pyridostigmine, an acetylcholinesterase inhibitor, has not been investigated. The objective of the present study was to determine the cardiac electrophysiologic effects of a single dose of pyridostigmine bromide in an open-label, quasi-experimental protocol. Fifteen patients who had been indicated for diagnostic cardiac electrophysiologic study underwent two studies just before and 90-120 min after the oral administration of pyridostigmine (45 mg). Pyridostigmine was well tolerated by all patients. Wenckebach nodal anterograde atrioventricular point and basic cycle were not altered by pyridostigmine. Sinus recovery time (ms) was shorter during a 500-ms cycle stimulation (pre: 326 ± 45 vs post:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac electrophysiology; Parasympathetic nervous system; Cholinesterase inhibitors; Cardiovascular disease; Pyridostigmine bromide; Autonomic nervous system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200013

TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines
Provedor de dados: 56
Autores: Dalla Torre,C.A.; Maciel,R.M.B.; Pinheiro,N.A.; Andrade,J.A.D.; de Toledo,S.R.C.; Villa,L.L.; Cerutti,J.M.
Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Telomeric repeat amplification protocol; TRAP assay; Human thyroid cancer; Leukemia; Telomerase; Silver staining.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100009

Superoxide release and cellular gluthatione peroxidase activity in leukocytes from children with persistent asthma
Provedor de dados: 56
Autores: Marçal,L.E.; Rehder,J.; Newburger,P.E.; Condino-Neto,A.
Asthma is an inflammatory condition characterized by the involvement of several mediators, including reactive oxygen species. The aim of the present study was to investigate the superoxide release and cellular glutathione peroxidase (cGPx) activity in peripheral blood granulocytes and monocytes from children and adolescents with atopic asthma. Forty-four patients were selected and classified as having intermittent or persistent asthma (mild, moderate or severe). The spontaneous or phorbol myristate acetate (PMA, 30 nM)-induced superoxide release by granulocytes and monocytes was determined at 0, 5, 15, and 25 min. cGPx activity was assayed spectrophotometrically. The spontaneous superoxide release by granulocytes from patients with mild (N = 15), moderate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Asthma; Respiratory burst; Granulocytes; Monocytes; Inflammation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100003

The role of calcium, calcium-activated K+ channels, and tyrosine/kinase in psoralen-evoked responses in human melanoma cells
Provedor de dados: 56
Autores: Isoldi,M.C.; Pereira,E.A.; Visconti,M.A.; Castrucci,A.M.L.
8-Methoxy psoralen (8-MOP) exerts a short-term (24 h) mitogenic action, and a long-term (48-72 h) anti-proliferative and melanogenic action on two human melanoma cell lines, SK-Mel 28 and C32TG. An increase of intracellular calcium concentration was observed by spectrofluorometry immediately after the addition of 0.1 mM 8-MOP to both cell lines, previously incubated with calcium probe fluo-3 AM (5 µM). The intracellular Ca2+ chelator BAPTA/AM (1 µM) blocked both early (mitogenic) and late (anti-proliferative and melanogenic) 8-MOP effects on both cell lines, thus revealing the importance of the calcium signal in both short- and long-term 8-MOP-evoked responses. Long-term biological assays with 5 and 10 mM tetraethylammonium chloride (TEA, an inhibitor of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 8-[Methoxy]-psoralen; SK-Mel 28 cell line; C32TG cell line; Cell proliferation; Cell signaling; Melanoma.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400013

Anti-parasitic action and elimination of intracellular Toxoplasma gondii in the presence of novel thiosemicarbazone and its 4-thiazolidinone derivatives
Provedor de dados: 56
Autores: Carvalho,C.S.; de Melo,E.J.T.
Toxoplasma, which infects all eukaryotic cells, is considered to be a good system for the study of drug action and of the behavior of infected host cells. In the present study, we asked if thiosemicarbazone derivatives can be effective against tachyzoites and which morphological and ultrastructural features of host cells and parasites are associated with the destruction of Toxoplasma. The compounds were tested in infected Vero cell culture using concentration screens (0.1 to 20 mM). The final concentration of 1 mM was chosen for biological assay. The following results were obtained: 1) These new derivatives decreased T. gondii infection with an in vitro parasite IC50% of 0.2-0.7 mM, without a significant effect on host cells and the more efficient...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid [2-(benzylidene-hydrazone)-4-oxo-1; 3-thiazolidin-5-yl]acetic r-chloro-benzaldehyde thiosemicarbazone Tachyzoite Toxoplasma.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200004

Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method
Provedor de dados: 56
Autores: Li,Chao; Fu,Gongyu; Shi,Yaoqiang; Zhang,A-Mei; Xia,Xueshan; Fang,Yue; Mao,Xiaoqin; Jiang,Jie; Song,Yuzhu; Yang,Guangying.
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loop-mediated isothermal amplification; Polymerase chain reaction; Klebsiella pneumoniae; Novel specific gene; Specific; Sensitive method.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300609

Examining life-course influences on chronic disease: the Ribeirão Preto and São Luís birth cohort studies (Brazil)
Provedor de dados: 56
Autores: Batty,G.D.
More than any other low- and middle-income country, Brazil has the longest research tradition of establishing, maintaining and exploiting birth cohort studies. This research pedigree is highlighted in the present issue of the Brazilian Journal of Medical and Biological Research, which contains a series of twelve papers from the Ribeirão Preto and São Luis birth cohort studies from the Southeast and Northeast of Brazil, respectively. The topics covered in this raft of reports vary and include predictors of perinatal health and maternal risk factors, early life determinants of cardiovascular risk factors in childhood and adolescence, use of health services, and a description of dietary characteristics of young adults, amongst other topics. There is also a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Birth cohort; Chronic disease; Developing country; Epidemiology; Life-course.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900001

High levels of serum mannose-binding lectin are associated with the severity of clinical signs of leptospirosis
Provedor de dados: 56
Autores: Miranda,K.A.; Vasconcelos,L.R.S.; Coelho,L.C.B.B.; Lima Filho,J.L.; Cavalcanti,M.S.M.; Moura,P.
The clinical heterogeneity observed in leptospirosis may be associated with host factors or bacteria virulence. Human serum mannose-binding lectin (MBL) recognizes many pathogens, and low levels of this lectin are associated with susceptibility to infection. MBL is also implicated in the modulation of the inflammatory process. We determined the levels of serum MBL during leptospirosis infection. A double-antibody sandwich ELISA was used to detect the immunoreactive serum MBL. The ELISA plates were coated with monoclonal antibody to MBL and bound MBL or recombinant human MBL were detected by rabbit anti-human MBL serum. HRPO-conjugated goat anti-rabbit antibody was used for detection of the reaction. Two groups of patients seen at referral hospitals in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leptospirosis; Mannose-binding lectin; Hemorrhage; Cardiac alteration; Renal complication.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400007

Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats
Provedor de dados: 56
Autores: Rodrigues,L.P.; Iglesias,D.; Nicola,F.C.; Steffens,D.; Valentim,L.; Witczak,A.; Zanatta,G.; Achaval,M.; Pranke,P.; Netto,C.A.
Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 10(6) cells diluted in 10 µL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Cell therapy; Functional recovery; Mononuclear cells; Human umbilical cord blood; MASCIS impactor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100009

Effects of different frequencies of transcutaneous electrical nerve stimulation on venous vascular reactivity
Provedor de dados: 56
Autores: Franco,O.S.; Paulitsch,F.S.; Pereira,A.P.C.; Teixeira,A.O.; Martins,C.N.; Silva,A.M.V.; Plentz,R.D.M.; Irigoyen,M.C.; Signori,L.U.
Transcutaneous electrical nerve stimulation (TENS) is a type of therapy used primarily for analgesia, but also presents changes in the cardiovascular system responses; its effects are dependent upon application parameters. Alterations to the cardiovascular system suggest that TENS may modify venous vascular response. The objective of this study was to evaluate the effects of TENS at different frequencies (10 and 100 Hz) on venous vascular reactivity in healthy subjects. Twenty-nine healthy male volunteers were randomized into three groups: placebo (n=10), low-frequency TENS (10 Hz, n=9) and high-frequency TENS (100 Hz, n=10). TENS was applied for 30 min in the nervous plexus trajectory from the superior member (from cervical to dorsal region of the fist)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electric stimulation therapy; Vascular endothelium; Alpha-1 adrenergic receptors; Mechanical pain.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500411

Differential gene expression profiles of hepatocellular carcinomas associated or not with viral infection
Provedor de dados: 56
Autores: Bellodi-Privato,M.; Kubrusly,M.S.; Stefano,J.T.; Soares,I.C.; Wakamatsu,A.; Oliveira,A.C.; Alves,V.A.F.; Bacchella,T.; Machado,M.C.C.; D’Albuquerque,L.A.C.
Chronic hepatitis B (HBV) and C (HCV) virus infections are the most important factors associated with hepatocellular carcinoma (HCC), but tumor prognosis remains poor due to the lack of diagnostic biomarkers. In order to identify novel diagnostic markers and therapeutic targets, the gene expression profile associated with viral and non-viral HCC was assessed in 9 tumor samples by oligo-microarrays. The differentially expressed genes were examined using a z-score and KEGG pathway for the search of ontological biological processes. We selected a non-redundant set of 15 genes with the lowest P value for clustering samples into three groups using the non-supervised algorithm k-means. Fisher’s linear discriminant analysis was then applied in an exhaustive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Molecular biomarkers; Viral infection; Oligo-microarrays.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200001

Effects of septal cholinergic lesion on rat exploratory behavior in an open-field
Provedor de dados: 56
Autores: Lamprea,M.R.; Cardenas,F.P.; Silveira,R.; Walsh,T.J.; Morato,S.
The medial septum participates in the modulation of exploratory behavior triggered by novelty. Also, selective lesions of the cholinergic component of the septohippocampal system alter the habituation of rats to an elevated plus-maze without modifying anxiety indices. We investigated the effects of the intraseptal injection of the cholinergic immunotoxin 192 IgG-saporin (SAP) on the behavior of rats in an open-field. Thirty-nine male Wistar rats (weight: 194-230 g) were divided into three groups, non-injected controls and rats injected with either saline (0.5 µl) or SAP (237.5 ng/0.5 µl). Twelve days after surgery, the animals were placed in a square open-field (120 cm) and allowed to freely explore for 5 min. After the test, the rats were killed by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Exploratory behavior; Open-field; Cholinergic immunotoxin; Medial septum; Saporin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200011

A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide
Provedor de dados: 56
Autores: Carvalho,K.M.; Nava,R.A.; França,M.S.F.; Medeiros,M.A.S.; Camarão,G.C.; Juliano,L.
A new metalloendopeptidase was purified to apparent homogeneity from a homogenate of normal human liver using successive steps of chromatography on DEAE-cellulose, hydroxyapatite and Sephacryl S-200. The purified enzyme hydrolyzed the Pro7-Phe8 bond of bradykinin and the Ser25-Tyr26 bond of atrial natriuretic peptide. No cleavage was produced in other peptide hormones such as vasopressin, oxytocin or Met- and Leu-enkephalin. This enzyme activity was inhibited by 1 mM divalent cation chelators such as EDTA, EGTA and o-phenanthroline and was insensitive to 1 µM phosphoramidon and captopril, specific inhibitors of neutral endopeptidase (EC 3.4.24.11) and angiotensin-converting enzyme (EC 3.4.15.1), respectively. With Mr 85 kDa, the enzyme exhibits optimal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Liver metalloendopeptidase; Bradykinin; Atrial natriuretic peptide.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100007

Effects of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) release from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone
Provedor de dados: 56
Autores: Ren,Q.; Cai,M.; Zhang,K.; Ren,W.; Su,Z.; Yang,T.; Sun,T.; Wang,J.
Multiple growth factors can be administered to mimic the natural process of bone healing in bone tissue engineering. We investigated the effects of sequential release of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone regeneration. To improve the double emulsion/solvent evaporation technique, VEGF was encapsulated in PELA microcapsules, to which BMP-2 was attached. The scaffold (BMP-2/PELA/VEGF) was then fused to these microcapsules using the dichloromethane vapor method. The bioactivity of the released BMP-2 and VEGF was then quantified in rat mesenchymal stem cells (rMSCs). Immunoblotting analysis showed that BMP-2/PELA/VEG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone tissue engineering; BMP-2; VEGF; PELA; Scaffold.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200603

Cell lineage relationship in the stomach of normal and genetically manipulated mice
Provedor de dados: 56
Autores: Karam,S.M.
The oxyntic mucosa of the mouse stomach is lined with a heterogeneous population of cells that form numerous short pits continuous with long tubular glands. Tritiated thymidine radioautography has made it possible to pinpoint the origin of all cell types and to follow the differentiation/migration of different cell lineages along the pit-gland unit. The proliferating multipotent stem cells functionally anchored in the upper glandular region, the isthmus, give rise to three main lineage precursors: 1) pre-pit cells, which migrate upward to the pit while differentiating into mucus-producing pit cells; 2) pre-neck cells, which migrate downward to the glandular neck while differentiating into mucus-producing neck cells that, by approaching the glandular base,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioautography; Gastric epithelium; Stem cells; Cell proliferation/differentiation programs; Lineage progenitors; Transgenic mice.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200010

Immunoregulatory and anti-inflammatory effects of n-3 polyunsaturated fatty acids
Provedor de dados: 56
Autores: Calder,P.C.
1. Fish oils are rich in the long-chain n-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Linseed oil and green plant tissues are rich in the precursor fatty acid, &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;-linolenic acid (18:3n-3). Most vegetable oils are rich in the n-6 PUFA linoleic acid (18:2n-6), the precursor of arachidonic acid (20:4n-6). 2. Arachidonic acid-derived eicosanoids such as prostaglandin E2 are pro-inflammatory and regulate the functions of cells of the immune system. Consumption of fish oils leads to replacement of arachidonic acid in cell membranes by eicosapentaenoic acid. This changes the amount and alters the balance of eicosanoids produced. 3. Consumption of fish oils diminishes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocyte; Macrophage; Immune system; Inflammation; Fish oil; Polyunsaturated fatty acid; Cytokine; Eicosanoid.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400002

Control of the phosphorylation of the astrocyte marker glial fibrillary acidic protein (GFAP) in the immature rat hippocampus by glutamate and calcium ions: possible key factor in astrocytic plasticity
Provedor de dados: 56
The present review describes recent research on the regulation by glutamate and Ca2+ of the phosphorylation state of the intermediate filament protein of the astrocytic cytoskeleton, glial fibrillary acidic protein (GFAP), in immature hippocampal slices. The results of this research are discussed against a background of modern knowledge of the functional importance of astrocytes in the brain and of the structure and dynamic properties of intermediate filament proteins. Astrocytes are now recognized as partners with neurons in many aspects of brain function with important roles in neural plasticity. Site-specific phosphorylation of intermediate filament proteins, including GFAP, has been shown to regulate the dynamic equilibrium between the polymerized and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Astrocytes; Glial fibrillary acidic protein; GFAP; Glutamate receptors; Protein phosphorylation; Calcium; Intermediate filament dynamics.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300005

A comparison of public and private obstructive sleep apnea clinics
Provedor de dados: 56
Autores: Zonato,A.I.; Bittencourt,L.R.; Martinho,F.L.; Baiard,P.; Togeiro,S.M.; Benedito-Silva,A.A.; Tufik,S.
The aim of the present study was to compare the clinical findings and polysomnography results obtained at public and private clinics in Brazil, the follow-up after diagnosis, and the therapeutic aspects related to continuous positive airway pressure. Patients who snore and who have obstructive sleep apnea were retrospectively divided into two groups, i.e., public clinic (N = 307) and private clinic (N = 317). Data concerning age, sex, body mass index (BMI), neck circumference, medical history, sleepiness scale, follow-up after diagnosis, and acceptance of continuous positive airway pressure therapy were collected. Mean age was 50 ± 12 (range: 15-80) for public patients and 48 ± 12 years (range: 19-91) for private patients. Mean BMI was 30 ± 6 (range:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obstructive sleep apnea; Social class aspects; Anthropometric aspects; Continuous positive airway pressure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100010

Myocyte necrosis is the basis for fibrosis in  renovascular hypertensive rats
Provedor de dados: 56
Autores: Okoshi,M.P.; Matsubara,L.S.; Franco,M.; Cicogna,A.C.; Matsubara,B.B.
The pathogenesis of fibrosis and the functional features of pressure overload myocardial hypertrophy are still controversial. The objectives of the present study were to evaluate the function and morphology of the hypertrophied myocardium in renovascular hypertensive (RHT) rats. Male Wistar rats were sacrificed at week 4 (RHT4) and 8 (RHT8) after unilateral renal ischemia (Goldblatt II hypertension model). Normotensive rats were used as controls. Myocardial function was analyzed in isolated papillary muscle preparations, morphological features were defined by light microscopy, and myocardial hydroxyproline concentration (HOP) was determined by spectrophotometry. Renal artery clipping resulted in elevated systolic arterial pressure (RHT4: 178 ± 19 mmHg and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary vascular remodeling; Myocardial function; Pressure overload hypertrophy; Papillary muscle; Reparative fib.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900013

Characterization of the interdependency between residues that bind the substrate in a β-glycosidase
Provedor de dados: 56
Autores: Tomassi,M.H.; Rozenfeld,J.H.K.; Gonçalves,L.M.; Marana,S.R.
The manner by which effects of simultaneous mutations combine to change enzymatic activity is not easily predictable because these effects are not always additive in a linear manner. Hence, the characterization of the effects of simultaneous mutations of amino acid residues that bind the substrate can make a significant contribution to the understanding of the substrate specificity of enzymes. In the β-glycosidase from Spodoptera frugiperda (Sfβgly), both residues Q39 and E451 interact with the substrate and this is essential for defining substrate specificity. Double mutants of Sfβgly (A451E39, S451E39 and S451N39) were prepared by site-directed mutagenesis, expressed in bacteria and purified using affinity chromatography. These enzymes were characterized...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Β-glycosidase; Substrate specificity; Site-directed mutagenesis; Spodoptera frugiperda.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100002

Gastroduodenal resistance and neural mechanisms involved in saline flow decrease elicited by acute blood volume expansion in anesthetized rats
Provedor de dados: 56
Autores: Graça,J.R.V.; Gondim,F. de-A.A.; Cavalcante,D.I.M.; Xavier-Neto,J.; Messias,E.L.M.; Rego,M.C.V.; Marques,J.A.P.; Santos,A.A.; Rola,F.H.
We have previously demonstrated that blood volume (BV) expansion decreases saline flow through the gastroduodenal (GD) segment in anesthetized rats (Xavier-Neto J, dos Santos AA &amp;amp; Rola FH (1990) Gut, 31: 1006-1010). The present study attempts to identify the site(s) of resistance and neural mechanisms involved in this phenomenon. Male Wistar rats (N = 97, 200-300 g) were surgically manipulated to create four gut circuits: GD, gastric, pyloric and duodenal. These circuits were perfused under barostatically controlled pressure (4 cmH2O). Steady-state changes in flow were taken to reflect modifications in circuit resistances during three periods of time: normovolemic control (20 min), expansion (10-15 min), and expanded (30 min). Perfusion flow rates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood volume expansion gastroduodenal resistance duodenum pylorus !-- $MVD$:face("Symbol") --&lt; FONT FACE=Symbol&gt; A&lt; /font&gt; -blockers vagotomy.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000019

Intercostal and forearm muscle deoxygenation during respiratory fatigue in patients with heart failure: potential role of a respiratory muscle metaboreflex
Provedor de dados: 56
Autores: Moreno,A.M.; Castro,R.R.T.; Silva,B.M.; Villacorta,H.; Junior,M. Sant'Anna; Nóbrega,A.C.L.
The purpose of this study was to determine the effect of respiratory muscle fatigue on intercostal and forearm muscle perfusion and oxygenation in patients with heart failure. Five clinically stable heart failure patients with respiratory muscle weakness (age, 66±12 years; left ventricle ejection fraction, 34±3%) and nine matched healthy controls underwent a respiratory muscle fatigue protocol, breathing against a fixed resistance at 60% of their maximal inspiratory pressure for as long as they could sustain the predetermined inspiratory pressure. Intercostal and forearm muscle blood volume and oxygenation were continuously monitored by near-infrared spectroscopy with transducers placed on the seventh left intercostal space and the left forearm. Data were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Metaboreflex; Muscles respiratory.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100972

Comparison of angular frequency contrast sensitivity in young and older adults
Provedor de dados: 56
Autores: Santos,N.A.; Simas,M.L.B.; Nogueira,R.M.T.B.L.
The aim of the present study was to measure contrast sensitivity curves for angular frequencies in the range between 2 and 96 cycles/360º in older human adult volunteers and to compare these measurements with the more usual contrast sensitivity functions for sine-wave gratings. All subjects were free of identifiable ocular disease and had normal acuity. We measured the contrast thresholds for young adults (N = 6; age range, 20-26 years) and older adults (N = 6; age range, 60-67 years) using the psychophysical forced-choice method. In this paradigm the volunteers had to choose the stimulus containing a test frequency at low contrast (e.g., either a sine-wave grating or an angular frequency stimulus), or another neutral stimulus at mean luminance (without...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human aging; Spatial frequency; Angular frequency; Contrast sensitivity; Forced-choice method.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300014

Differential in vitro pathogenicity of predatory fungi of the genus Monacrosporium for phytonematodes, free-living nematodes and parasitic nematodes of cattle
Provedor de dados: 56
Autores: Gomes,A.P.S.; Araújo,J.V.; Ribeiro,R.C.F.
In vitro tests were carried out on the pathogenicity of nine isolates of the predatory fungi of the genus Monacrosporium (5 M. sinense isolates, 3 M. appendiculatum and 1 M. thaumasium isolate) for a phytonematode (second stage juveniles from Meloidogyne incognita, race 3), a free-living nematode (Panagrellus spp), and two gastrointestinal parasitic nematodes of cattle (infective larvae of Cooperia punctata and Haemonchus placei). A suspension containing 2,000 nematodes from each species was added to Petri dishes containing fungi and grown on 2% water-agar medium at 25oC in the dark for up to 7 days. The dishes were examined every other day for 7 days and predation-free nematodes were counted. The results showed that the free-living nematodes, Panagrellus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nematophagous fungi nematode-trapping fungus predacious fungi Monacrosporium; Nematode Cooperia punctata Haemonchus placei Meloidogyne incognita Panagrellus.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100012

Effect of the time-of-day of training on explicit memory
Provedor de dados: 56
Autores: Barbosa,F.F.; Albuquerque,F.S.
Studies have shown a time-of-day of training effect on long-term explicit memory with a greater effect being shown in the afternoon than in the morning. However, these studies did not control the chronotype variable. Therefore, the purpose of this study was to assess if the time-of-day effect on explicit memory would continue if this variable were controlled, in addition to identifying the occurrence of a possible synchronic effect. A total of 68 undergraduates were classified as morning, intermediate, or afternoon types. The subjects listened to a list of 10 words during the training phase and immediately performed a recognition task, a procedure which they repeated twice. One week later, they underwent an unannounced recognition test. The target list and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory; Circadian rhythms; Explicit memory; Recognition memory.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600007

Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
Provedor de dados: 56
Autores: Wang,Y.F.; Wu,M.; Ma,B.J.; Cai,D.A.; Yin,B.B.
In this study, we investigated the potential role of high-mobility group box 1 (HMGB1) in severe acute pancreatitis (SAP) and the effects of growth hormone (G) and somatostatin (S) in SAP rats. The rats were randomly divided into 6 groups of 20 each: sham-operated, SAP, SAP+saline, SAP+G, SAP+S and SAP+G+S. Ileum and pancreas tissues of rats in each group were evaluated histologically. HMGB1 mRNA expression was measured by reverse transcription-PCR. Levels of circulating TNF-α, IL-1, IL-6, and endotoxin were also measured. In the SAP group, interstitial congestion and edema, inflammatory cell infiltration, and interstitial hemorrhage occurred in ileum and pancreas tissues. The levels of HMGB1, TNF-α, IL-1, IL-6 and endotoxin were significantly up-regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Severe acute pancreatitis; High-mobility group box 1; Growth hormone; Somatostatin; Inflammatory response.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201075

Poly-DL-lactide-co-glycolide microspheres as a controlled release antigen delivery system
Provedor de dados: 56
Autores: Lima,K.M.; Rodrigues Júnior,J.M.
Successful vaccine application means maximum protection with minimal number of administrations. A rational development of vaccines involves studies of the nature of the antigen as well as of the adjuvant to be used to improve the immune responses. This has provided the impetus for studies to design the degradable devices and for different approaches to antigen delivery by different routes of administration. The development of controlled release systems based on polymeric devices that permit a sustained or pulsed release of encapsulated antigens has attracted much interest. Polymeric delivery systems consist of polymers that release their content continuously in a controlled manner over a period of time. The development of a biocompatible delivery system...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccine; Adjuvant; Microspheres; Microencapsulation; Biomaterial.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200005

Retinol-induced changes in the phosphorylation levels of histones and high mobility group proteins from Sertoli cells
Provedor de dados: 56
Autores: Moreira,J.C.F.; Dal-Pizzol,F.; Rocha,A.B.; Klamt,F.; Ribeiro,N.C.; Ferreira,C.J.S.; Bernard,E.A.
Chromatin proteins play a role in the organization and functions of DNA. Covalent modifications of nuclear proteins modulate their interactions with DNA sequences and are probably one of the multiple factors involved in the process of switch on/off transcriptionally active regions of DNA. Histones and high mobility group proteins (HMG) are subject to many covalent modifications that may modulate their capacity to bind to DNA. We investigated the changes induced in the phosphorylation pattern of cultured Wistar rat Sertoli cell histones and high mobility group protein subfamilies exposed to 7 µM retinol for up to 48 h. In each experiment, 6 h before the end of the retinol treatment each culture flask received 370 KBq/ml [32P]-phosphate. The histone and HMGs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sertoli cells; Phosphorylation; Histones; High mobility group proteins; Retinol.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300005

Regulation of transgene expression in genetic immunization
Provedor de dados: 56
Autores: Harms,J.S.; Oliveira,S.C.; Splitter,G.A.
The use of mammalian gene expression vectors has become increasingly important for genetic immunization and gene therapy as well as basic research. Essential for the success of these vectors in genetic immunization is the proper choice of a promoter linked to the antigen of interest. Many genetic immunization vectors use promoter elements from pathogenic viruses including SV40 and CMV. Lymphokines produced by the immune response to proteins expressed by these vectors could inhibit further transcription initiation by viral promoters. Our objective was to determine the effect of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt; on transgene expression driven by viral SV40 or CMV promoter/enhancer and the mammalian promoter/enhancer for the major...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccine; Gene therapy; Regulation; Transcription; Transgene.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200003

The radioprotective effect of a new aminothiol (20-PRA)
Provedor de dados: 56
Autores: Dolabela,M.F.; Pereira,M.T.; Salas,C.E.; Steffani,G.M.; Nelson,D.L.; Piló-Veloso,D.; Lopes,M.T.P.
We examined the radioprotective effect of aminothiol 2-N-propylamine-cyclo-hexanethiol (20-PRA) on a human leukemic cell line (K562) following various radiation doses (5, 7.5 and 20 Gy) using a source of 60Co &lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;-rays. At 5 Gy and 1 nM 20-PRA, a substantial protective effect (58%) was seen 24 h after irradiation, followed by a decrease at 48 h (11%). At the high radiation dose (20 Gy) a low protective effect was also seen (35%). In addition, the antitumorigenic potential of 10 nM 20-PRA was shown by the inhibition of crown gall formation induced by Agrobacterium tumefaciens. The radioprotective potency of 20-PRA is 105-106 times higher than that of the aminothiol WR-1065 (N-(2-mercaptoethyl)-1,3-diaminopropane) whose...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aminothiols; Cytotoxic effect; Radioprotector; Antitumorigenic effect.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800010

Rats with high left ventricular end-diastolic pressure can be identified by Doppler echocardiography one week after myocardial infarction
Provedor de dados: 56
Autores: Saraiva,R.M.; Kanashiro-Takeuchi,R.M.; Antonio,E.L.; Campos,O.; Tucci,P.J.F.; Moisés,V.A.
The severity of left ventricular (LV) dysfunction in rats with myocardial infarction (MI) varies widely. Because homogeneity in baseline parameters is essential for experimental investigations, a study was conducted to establish whether Doppler echocardiography (DE) could accurately identify animals with high LV end-diastolic pressure as a marker of LV dysfunction soon after MI. Direct measurements of LV end-diastolic pressure were made and DE was performed simultaneously 1 week after surgically induced MI (N = 16) or sham-operation (N = 17) in female Wistar rats (200 to 250 g). The ratio of peak early (E) to late (A) diastolic LV filling velocities and the ratio of E velocity to peak early (Em) diastolic myocardial velocity were the best predictors of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Myocardial infarction; Echocardiography; Hemodynamics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100016

Identification of a protein kinase activity that phosphorylates connexin43 in a pH-dependent manner
Provedor de dados: 56
Autores: Yahuaca,P.; Ek-Vitorin,J.F.; Rush,P.; Delmar,M.; Taffet,S.M.
The carboxyl-terminal (CT) domain of connexin43 (Cx43) has been implicated in both hormonal and pH-dependent gating of the gap junction channel. An in vitro assay was utilized to determine whether the acidification of cell extracts results in the activation of a protein kinase that can phosphorylate the CT domain. A glutathione S-transferase (GST)-fusion protein was bound to Sephadex beads and used as a target for protein kinase phosphorylation. A protein extract produced from sheep heart was allowed to bind to the fusion protein-coated beads. The bound proteins were washed and then incubated with 32P-ATP. Phosphorylation was assessed after the proteins were resolved by SDS-PAGE. Incubation at pH 7.5 resulted in a minimal amount of phosphorylation while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Connexin; Phosphorylation; Phosphotransferases; Protein kinase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400005

Cardiopulmonary bypass alters the pharmacokinetics of propranolol in patients undergoing cardiac surgery
Provedor de dados: 56
Autores: Carmona,M.J.C.; Malbouisson,L.M.S.; Pereira,V.A.; Bertoline,M.A.; Omosako,C.E.K.; Le Bihan,K.B.; Auler Jr.,J.O.C.; Santos,S.R.C.J.
The pharmacokinetics of propranolol may be altered by hypothermic cardiopulmonary bypass (CPB), resulting in unpredictable postoperative hemodynamic responses to usual doses. The objective of the present study was to investigate the pharmacokinetics of propranolol in patients undergoing coronary artery bypass grafting (CABG) by CPB under moderate hypothermia. We evaluated 11 patients, 4 women and 7 men (mean age 57 ± 8 years, mean weight 75.4 ± 11.9 kg and mean body surface area 1.83 ± 0.19 m²), receiving propranolol before surgery (80-240 mg a day) and postoperatively (10 mg a day). Plasma propranolol levels were measured before and after CPB by high-performance liquid chromatography. Pharmacokinetic Solutions 2.0 software was used to estimate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propranolol; Pharmacokinetics; Cardiopulmonary bypass.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500008

Anti-Leishmania titers and positive skin tests in patients cured of kala-azar
Provedor de dados: 56
Autores: Viana,G.M.C.; Nascimento,M.D.S.B.; Diniz Neto,J.A.; Rabelo,E.M.F.; Binda Júnior,J.R.; Santos Júnior,O.M.; Santos,A.C.; Galvão,C.S.; Guimarães,R.S.
Visceral leishmaniasis (VL), also known as kala-azar, is an important public health problem. If not treated, virtually all clinically symptomatic patients die within months. The diagnosis is based on the Montenegro skin test (MST) and anti-Leishmania titers. Nevertheless, the time required for cured individuals living in a leishmaniasis-endemic area to present a positive skin test and negative anti-Leishmania serology is known. To determine the cellular and humoral immune response profile in relation to different times post-VL cure, a cross-sectional study was conducted on subjects from a kala-azar endemic area in Paço do Lumiar, MA, Brazil, on the basis of 1995-2005 notifications reported by the National Health Foundation/Regional Coordination of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human visceral leishmaniasis; Montenegro’s intradermal reaction; Indirect immunofluorescence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100009

Combined treatment with caffeic and ferulic acid from Baccharis uncinella C. DC. (Asteraceae) protects against metabolic syndrome in mice
Provedor de dados: 56
Autores: Bocco,B.M.; Fernandes,G.W.; Lorena,F.B.; Cysneiros,R.M.; Christoffolete,M.A.; Grecco,S.S.; Lancellotti,C.L.; Romoff,P.; Lago,J.H.G.; Bianco,A.C.; Ribeiro,M.O.
Fractionation of the EtOH extract from aerial parts of Baccharis uncinella C. DC. (Asteraceae) led to isolation of caffeic and ferulic acids, which were identified from spectroscopic and spectrometric evidence. These compounds exhibit antioxidant and anti-inflammatory properties and have been shown to be effective in the prevention/treatment of metabolic syndrome. This study investigated whether the combined treatment of caffeic and ferulic acids exhibits a more significant beneficial effect in a mouse model with metabolic syndrome. The combination treatment with caffeic and ferulic acids was tested for 60 days in C57 mice kept on a high-fat (40%) diet. The data obtained indicated that treatment with caffeic and ferulic acids prevented gain in body weight...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Caffeic acid; Ferulic acid; Metabolic syndrome.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300604

Effect of lncRNA HULC knockdown on rat secreting pituitary adenoma GH3 cells
Provedor de dados: 56
Autores: Rui,Qiu Hong; Ma,Jian Bo; Liao,Yu Feng; Dai,Jin Hua; Cai,Zhen Yu.
Pituitary adenoma is one of the most common tumors in the neuroendocrine system. This study investigated the effects of long non-coding RNAs (lncRNAs) highly up-regulated in liver cancer (HULC) on rat secreting pituitary adenoma GH3 cell viability, migration, invasion, apoptosis, and hormone secretion, as well as the underlying potential mechanisms. Cell transfection and qRT-PCR were used to change and measure the expression levels of HULC, miR-130b, and FOXM1. Cell viability, migration, invasion, and apoptosis were assessed using trypan blue staining assay, MTT assay, two-chamber transwell assay, Guava Nexin assay, and western blotting. The concentrations of prolactin (PRL) and growth hormone (GH) in culture supernatant of GH3 cells were assessed using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Secreting pituitary adenoma; LncRNA highly up-regulated in liver cancer (HULC); MicroRNA-130b; Forkhead box protein M1; PI3K/AKT/mTOR signaling pathway; JAK1/STAT3 signaling pathway.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400610

Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation
Provedor de dados: 56
Autores: Mermelstein,C.S.; Rebello,M.I.L.; Amaral,L.M.; Costa,M.L.
Although much is known about the molecules involved in extracellular Ca2+ regulation, the relationship of the ion with overall cell morphology is not understood. The objective of the present study was to determine the effect of the Ca2+ chelator EGTA on the major cytoskeleton components, at integrin-containing adhesion sites, and their consequences on cell shape. Control mouse cell line C2C12 has a well-spread morphology with long stress fibers running in many different directions, as detected by fluorescence microscopy using rhodamine-phalloidin. In contrast, cells treated with EGTA (1.75 mM in culture medium) for 24 h became bipolar and showed less stress fibers running in one major direction. The adhesion plaque protein alpha5-integrin was detected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytoskeleton; Extracellular Ca2+; Cell shape; Microfilaments; Microtubules; Integrin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800018

Physiological implications of the regulation of vacuolar H+-ATPase by chloride ions
Provedor de dados: 56
Autores: Carraro-Lacroix,L.R.; Lessa,L.M.A.; Fernandez,R.; Malnic,G.
Vacuolar H+-ATPase is a large multi-subunit protein that mediates ATP-driven vectorial H+ transport across the membranes. It is widely distributed and present in virtually all eukaryotic cells in intracellular membranes or in the plasma membrane of specialized cells. In subcellular organelles, ATPase is responsible for the acidification of the vesicular interior, which requires an intraorganellar acidic pH to maintain optimal enzyme activity. Control of vacuolar H+-ATPase depends on the potential difference across the membrane in which the proton ATPase is inserted. Since the transport performed by H+-ATPase is electrogenic, translocation of H+-ions across the membranes by the pump creates a lumen-positive voltage in the absence of a neutralizing current,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H+-ATPase; Chloride channels; Cl-/H+ Antiporter; Intracellular organelles; Plasma membranes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200002

Intravenous regional block is similar to sympathetic ganglion block for pain management in patients with complex regional pain syndrome type I
Provedor de dados: 56
Autores: Nascimento,M.S.A.; Klamt,J.G.; Prado,W.A.
Sympathetic ganglion block (SGB) or intravenous regional block (IVRB) has been recommended for pain management in patients with complex regional pain syndrome type I (CRPS-I). Forty-five patients were initially selected but only 43 were accepted for the study. The present study evaluated the efficacy of IVRB produced by combining 70 mg lidocaine with 30 µg clonidine (14 patients, 1 male/13 females, age range: 27-50 years) versus SGB produced by the injection of 70 mg lidocaine alone (14 patients, 1 male/13 females, age range: 27-54 years) or combined with 30 µg clonidine (15 patients, 1 male/14 females, age range: 25-50 years) into the stellate ganglion for pain management in patients with upper extremity CRPS-I. Each procedure was repeated five times at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clonidine; Complex regional pain syndrome type I; Intravenous regional block; Lidocaine; Stellate ganglion block; Sympathetic ganglion block.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200014

Salvinorin A preserves cerebral pial artery autoregulation after forebrain ischemia via the PI3K/AKT/cGMP pathway
Provedor de dados: 56
Autores: Dong,H.P.; Zhou,W.; Ma,X.X.; He,Z.Z.; Wang,Z.H.
This study aimed to investigate the protective effect of salvinorin A on the cerebral pial artery after forebrain ischemia and explore related mechanisms. Thirty Sprague-Dawley rats received forebrain ischemia for 10 min. The dilation responses of the cerebral pial artery to hypercapnia and hypotension were assessed in rats before and 1 h after ischemia. The ischemia reperfusion (IR) control group received DMSO (1 µL/kg) immediately after ischemia. Two different doses of salvinorin A (10 and 20 µg/kg) were administered following the onset of reperfusion. The 5th, 6th, and 7th groups received salvinorin A (20 µg/kg) and LY294002 (10 µM), L-NAME (10 μM), or norbinaltorphimine (norBIN, 1 μM) after ischemia. The levels of cGMP in the cerebrospinal fluid (CSF)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salvinorin A; Cerebral pial artery; Dilation; Forebrain ischemia; PI3K/AKT/cGMP pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500604

Central actions of glucocorticoids in the control of body fluid homeostasis: Review
Provedor de dados: 56
Autores: Ruginsk,S.G.; Lopes da Silva,A.; Ventura,R.R.; Elias,L.L.K.; Antunes-Rodrigues,J.
The involvement of the hypothalamic-pituitary-adrenal axis in the control of body fluid homeostasis has been extensively investigated in the past few years. In the present study, we reviewed the recent results obtained using different approaches to investigate the effects of glucocorticoids on the mechanisms of oxytocin and vasopressin synthesis and secretion in response to acute and chronic plasma volume and osmolality changes. The data presented here suggest that glucocorticoids are not only involved in the mechanisms underlying the fast release but also in the transcriptional events that lead to decreased synthesis and secretion of these neuropeptides, particularly oxytocin, under diverse experimental conditions of altered fluid volume and tonicity. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corticosterone; Vasopressin; Oxytocin; Endocannabinoid; Glutamate; NFκB.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100010

Alternative mitochondrial functions in cell physiopathology: beyond ATP production
Provedor de dados: 56
Autores: Kowaltowski,A.J.
It is well known that mitochondria are the main site for ATP generation within most tissues. However, mitochondria also participate in a surprising number of alternative activities, including intracellular Ca2+ regulation, thermogenesis and the control of apoptosis. In addition, mitochondria are the main cellular generators of reactive oxygen species, and may trigger necrotic cell death under conditions of oxidative stress. This review concentrates on these alternative mitochondrial functions, and their role in cell physiopathology.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial permeability transition; Uncoupling proteins; Alternative oxidase; Cardioprotection; Free radicals; Aging.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200014

Opposite lipemic response of Wistar rats and C57BL/6 mice to dietary glucose or fructose supplementation
Provedor de dados: 56
Autores: Barbosa,C.R.; Albuquerque,E.M.V.; Faria,E.C.; Oliveira,H.C.F.; Castilho,L.N.
The metabolic effects of carbohydrate supplementation in mice have not been extensively studied. In rats, glucose- and fructose-rich diets induce hypertriacylglycerolemia. In the present study, we compared the metabolic responses to two monosaccharide supplementations in two murine models. Adult male Wistar rats (N = 80) and C57BL/6 mice (N = 60), after 3 weeks on a standardized diet, were submitted to dietary supplementation by gavage with glucose (G) or fructose (F) solutions (500 g/L), 8 g/kg body weight for 21 days. Glycemia was significantly higher in rats after fructose treatment (F: 7.9 vs 9.3 mM) and in mice (G: 6.5 vs 10 and F: 6.6 vs 8.9 mM) after both carbohydrate treatments. Triacylglycerolemia increased significantly 1.5 times in rats after G...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose or fructose supplementation; Triacylglycerolemia; Insulin resistance; C57BL/6 mice; Wistar rats.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300007

Two research paths for probing the roles of oxygen in metabolic regulation
Provedor de dados: 56
Autores: Hochachka,P.W.
Tissues such as skeletal and cardiac muscles must sustain very large-scale changes in ATP turnover rate during equally large changes in work. In many skeletal muscles these changes can exceed 100-fold. Examination of a number of cell and whole-organism level systems identifies ATP concentration as a key parameter of the interior milieu that is nearly universally 'homeostatic'; it is common to observe no change in ATP concentration even while change in its turnover rate can increase or decrease by two orders of magnitude or more. A large number of other intermediates of cellular metabolism are also regulated within narrow concentration ranges, but none seemingly as precisely as is [ATP]. In fact, the only other metabolite in aerobic energy metabolism that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic regulation; Oxygen delivery; Oxygen regulation; Intracellular perfusion; Intracellular diffusion.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600001

Increased immunohistochemical expression of YKL-40 in the spleen of patients with portal hypertension
Provedor de dados: 56
Autores: Wang,Dong; Lu,Jian-Guo; Wang,Qing; Du,Xi-Lin; Dong,Rui; Wang,Peng; Zhao,Lei; Jiang,Xue; Yuan,Li-Juan.
YKL-40 has been identified as a growth factor in connective tissue cells and also a migration factor in vascular smooth muscle cells. To a large extent, the increase of serum YKL-40 is attributed to liver fibrosis and asthma. However, the relationship of the expression and clinical/prognostic significance of YKL-40 to the splenomegaly of patients with portal hypertension is unclear. In the present study, the expression of YKL-40 was studied by immunohistochemistry in 48 splenomegaly tissue samples from patients with portal hypertension and in 14 normal spleen specimens. All specimens were quickly stored at -80°C after resection. Primary antibodies YKL-40 (1:150 dilution, rabbit polyclonal IgG) and MMP-9 (1:200 dilution, rabbit monoclonal IgG) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YKL-40; MMP-9; Splenomegaly; Fibrosis; Portal hypertension; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300013

Cholesterol changes the fatty acid composition of rat enterocytes
Provedor de dados: 56
Autores: Gazzola,J.; Martins,E.F.; Miyasaka,C.K.; Palanch,A.C.; Vecchia,M.G.; Curi,R.
The effect of free cholesterol on the fatty acid composition and growth of rat fetal enterocytes was investigated in the absence and presence of 10% (v/v) fetal calf serum. Cholesterol caused a significant reduction of cell number after 6 and 12 h in culture. The fatty acid composition of enterocytes cultured in the presence of serum was also changed by the presence of 20 µM cholesterol. The fatty acid profile was determined by HPLC using fluorescence detection (325 nm excitation and 395 nm emission). Cholesterol (20 µM) increased the proportion (given in percentage of the total fatty acids) of the following fatty acids in cultured cells: lauric (by 42%), oleic (by 34%), linoleic (by 44%) and gamma-linolenic (by 20%) acids and reduced the proportion of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterocytes; Cholesterol; Fatty acid composition; Cell proliferation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100019

Uniform decrease of alpha-global field power induced by intermittent photic stimulation of healthy subjects
Provedor de dados: 56
Autores: Rau,R.; Raschka,C.; Koch,H.J.
Nineteen-channel EEGs were recorded from the scalp surface of 30 healthy subjects (16 males and 14 females, mean age: 34 years, SD: 11.7 years) at rest and under trains of intermittent photic stimulation (IPS) at rates of 5, 10 and 20 Hz. Digitalized data were submitted to spectral analysis with fast fourier transformation providing the basis for the computation of global field power (GFP). For quantification, GFP values in the frequency ranges of 5, 10 and 20 Hz at rest were divided by the corresponding data obtained under IPS. All subjects showed a photic driving effect at each rate of stimulation. GFP data were normally distributed, whereas ratios from photic driving effect data showed no uniform behavior due to high interindividual variability....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EEG; Brain mapping; Intermittent photic stimulation; IPS; Global field power ratios.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500014

Brazilian Journal of Medical and Biological Research 1981-2002
Provedor de dados: 56
Autores: Krieger,Eduardo Moacyr; Ferreira,Sérgio Henrique.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100001

Strontium ranelate as a possible disease-modifying osteoarthritis drug: a systematic review
Provedor de dados: 56
Autores: Rodrigues,T.A.; Freire,A.O.; Bonfim,B.F.; Cartágenes,M.S.S.; Garcia,J.B.S.
Considering that osteoarthritis (OA) is the most prevalent joint disease worldwide, multiple pharmacological treatments have been proposed to alter the articular structure with potential benefit in the progression of the disease. The so-called disease-modifying OA drugs have been frequently investigated but conclusive findings are rare. Strontium ranelate (SrRan) is a drug usually prescribed to treat osteoporosis, with proven effects in decreasing the risk of fractures and possible effect in reducing the progression of OA. The objective of this review was to demonstrate the current panorama of knowledge on the use of SrRan in clinical and experimental models, clarifying its mechanisms of action and describing possible anti-nociceptive and anti-inflammatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoarthritis; Strontium ranelate; Treatment; Pain; Symptoms.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800302

Does the incremental shuttle walk test require maximal effort in young obese women?
Provedor de dados: 56
Autores: Jürgensen,S.P.; Trimer,R.; Di Thommazo-Luporini,L.; Dourado,V.Z.; Bonjorno-Junior,J.C.; Oliveira,C.R.; Arena,R.; Borghi-Silva,A.
Obesity is a chronic disease with a multifaceted treatment approach that includes nutritional counseling, structured exercise training, and increased daily physical activity. Increased body mass elicits higher cardiovascular, ventilatory and metabolic demands to varying degrees during exercise. With functional capacity assessment, this variability can be evaluated so individualized guidance for exercise training and daily physical activity can be provided. The aim of the present study was to compare cardiovascular, ventilatory and metabolic responses obtained during a symptom-limited cardiopulmonary exercise test (CPX) on a treadmill to responses obtained by the incremental shuttle walk test (ISWT) in obese women and to propose a peak oxygen consumption...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Incremental shuttle walk test; Exercise; Obesity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800703

Esophageal striated muscle contractions in patients with Chagas' disease and idiopathic achalasia
Provedor de dados: 56
Autores: Dantas,R.O.; Aprile,L.R.O.; Aben-Athar,C.G.; Miranda,A.L.M.
Chagas' disease causes degeneration and reduction of the number of intrinsic neurons of the esophageal myenteric plexus, with consequent absent or partial lower esophageal sphincter relaxation and loss of peristalsis in the esophageal body. The impairment of esophageal motility is seen mainly in the distal smooth muscle region. There is no study about esophageal striated muscle contractions in the disease. In 81 patients with heartburn (44 with esophagitis) taken as controls, 51 patients with Chagas' disease (21 with esophageal dilatation) and 18 patients with idiopathic achalasia (11 with esophageal dilatation) we studied the amplitude, duration and area under the curve of esophageal proximal contractions. Using the manometric method and a continuous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal contractions; Chagas' disease; Idiopathic achalasia; Esophageal striated muscle.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600007

Photodynamic antimicrobial chemotherapy (PACT) for the treatment of malaria, leishmaniasis and trypanosomiasis
Provedor de dados: 56
Autores: Baptista,M.S.; Wainwright,M.
A photodynamic effect occurs when photosensitiser molecules absorb light and dissipate the absorbed energy by transferring it to biological acceptors (usually oxygen), generating an excess of reactive species that are able to force cells into death pathways. Several tropical diseases present physiopathological aspects that are accessible to the application of a photosensitiser and local illumination. In addition, disease may be transmitted through infected blood donations, and many of the aetiological agents associated with tropical diseases have been shown to be susceptible to the photodynamic approach. However, there has been no systematic investigation of the application of photoantimicrobial agents in the various presentations, whether to human disease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical diseases; Photodynamic therapy; Singlet oxygen; Photosensitisers; Leishmania; Chagas’ disease.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100001

Role of sensory nervous system vasoactive peptides in hypertension
Provedor de dados: 56
Autores: Watson,R.E.; Supowit,S.C.; Zhao,H.; Katki,K.A.; DiPette,D.J.
The goal of the present research was to elucidate the roles and mechanisms by which the sensory nervous system, through the actions of potent vasodilator neuropeptides, regulates cardiovascular function in both the normal state and in the pathophysiology of hypertension. The animal models of acquired hypertension studied were deoxycorticosterone-salt (DOC-salt), subtotal nephrectomy-salt (SN-salt), and Nomega-nitro-L-arginine methyl ester (L-NAME)-induced hypertension during pregnancy in rats. The genetic model was the spontaneously hypertensive rat (SHR). Calcitonin gene-related peptide (CGRP) and substance P (SP) are potent vasodilating neuropeptides. In the acquired models of hypertension, CGRP and SP play compensatory roles to buffer the blood pressure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Calcitonin gene-related peptide; Substance P; Neurons (afferent).
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900004

Efficacy of HUMN criteria for scoring the micronucleus assay in human lymphocytes exposed to a low concentration of p,p'-DDT
Provedor de dados: 56
Autores: Garaj-Vrhovac,V.; Gajski,G.; Ravlić,S.
The cytokinesis-block micronucleus (CBMN) assay is one of the standard cytogenetic tools employed to assess chromosomal damage subsequent to exposure to genotoxic/cytotoxic agents, and is widely applicable to plant, animal and human cells. In the present study, the CBMN assay was used to assess the baseline damage in binuclear human peripheral blood lymphocytes exposed to 25 µg/L p,p'-DDT for 1, 2, 24, and 48 h by measuring the frequency of micronuclei, nucleoplasmic bridges and nuclear buds. These new scoring criteria facilitated the detection of different types of clastogenic and aneugenic effects induced by this type of pollutant. With these criteria, CBMN can also be used to measure nucleoplasmic bridges which are considered to be consequences of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: P; P'-DDT Peripheral blood human lymphocytes Micronucleus assay Nucleoplasmic bridges Nuclear buds.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600006

High-impact exercise in rats prior to and during suspension can prevent bone loss
Provedor de dados: 56
Autores: Yanagihara,G.R.; Paiva,A.G.; Gasparini,G.A.; Macedo,A.P.; Frighetto,P.D.; Volpon,J.B.; Shimano,A.C.
High-impact exercise has been considered an important method for treating bone loss in osteopenic experimental models. In this study, we investigated the effects of osteopenia caused by inactivity in femora and tibiae of rats subjected to jump training using the rat tail suspension model. Eight-week-old female Wistar rats were divided into five groups (n=10 each group): jump training for 2 weeks before suspension and training during 3 weeks of suspension; jump training for 2 weeks before suspension; jump training only during suspension; suspension without any training; and a control group. The exercise protocol consisted of 20 jumps/day, 5 days/week, with a jump height of 40 cm. The bone mineral density of the femora and tibiae was measured by double...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone; Mechanical properties; Bone mineral density; Scanning electron microscopy; Jump; Rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300605

131I-induced changes in rat thyroid gland function
Provedor de dados: 56
Autores: Torlak,V.; Zemunik,T.; Modun,D.; Capkun,V.; Pesutic-Pisac,V.; Markotic,A.; Pavela-Vrancic,M.; Stanicic,A.
Therapeutic doses of 131I administered to thyrotoxic patients may cause thyroid failure. The present study used a rat model to determine thyroid function after the administration of different doses of 131I (64-277 µCi). Thirty male Fisher rats in the experimental group and 30 in the control group (untreated) were followed for 6 months. The animals were 4 months old at the beginning of the experiment and were sacrificed at an age of 9 months. Hormone concentration was determined before 131I administration (4-month-old animals) and three times following 131I administration, when the animals were 7, 8, and 9 months old. The thyroid glands were removed and weighed, their volume was determined and histopathological examination was performed at the end of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid gland; 131I; Hormones; Histopathology; T3; T4; TSH.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800009