Consulta: subjectFacets:"Embryo"
Registros recuperados: 67
Data/hora: 14/06/2024 13:22:59

Composición y remoción de nutrimentos y calidad del fruto de mango (Mangifera indica L.) en diferentes ambientes.
Provedor de dados: 32
Autores: Mellado Vázquez, Adriana.
Se estudió la influencia de las condiciones de cultivo (clima, riego o temporal y tipo de suelo) en la composición nutrimental de los tejidos del fruto (epicarpio, pulpa, testa y embrión) y en la remoción de nutrimentos por tejido y por tonelada de fruto fresco de los cvs. Ataulfo, Kent, Keitt y Tommy Atkins. Asimismo, en ‘Ataulfo’ y ‘Tommy Atkins’ se estudió la relación entre el contenido nutrimental en suelo, hojas, epicarpio y pulpa (materia seca) y pulpa fresca, con la calidad postcosecha. Se trabajó con árboles altamente productivos (producción superior a los 150 kg·árbol-1), seleccionados en el 2008 de los que se cosecharon frutos en madurez fisiológica. En la materia seca de cada tejido se determinó la concentración de: N, P, K, Ca, Mg, S, Fe, Cu,...
Palavras-chave: Mangifera indica; Epicarpio; Pulpa; Testa; Embrión; Calidad postcosecha; Skin; Pulp; Seed coat; Embryo; Post-harvest quality; Doctorado; Fruticultura.
Ano: 2012
URL: http://hdl.handle.net/10521/736

Estudios anatómicos fisiológicos y nutrimentales de semillas de heliconias
Provedor de dados: 32
Autores: Benítez Domínguez, Lizeth.
Las heliconias son especies que desempeñan importantes funciones en los ecosistemas y que tienen un valor comercial alto debido a sus vistosas inflorescencias. Estas especies normalmente se reproducen de manera asexual y su producción se reduce a manejos tradicionales, por lo que existen pocos estudios de sus semillas y del manejo nutrimental de las plantas para elevar su producción. En esta investigación de hicieron estudios anatómicos, fisiológico y nutrimentales de cinco especies de heliconias: Heliconia bourgaeana, H. collinsiana, H. latispatha, H. psittacorum y H. rostrata. Las semillas de estas especies resultaron impermeables al agua. En H. collinsiana y H. latispatha los embriones se encontraron bien desarrollados y sin daños después de un año de...
Palavras-chave: Heliconiaceae; Embrión; Endospermo; Ácido abscísico; Nutrimentos; Heliconiaceae; Embryo; Endosperm; Abscisic acid; Nutrients; Maestría; Edafología.
Ano: 2010
URL: http://hdl.handle.net/10521/80

Regeneración de plantas de chile habanero (Capsicum chinense Jaqc.) a partir de cultivos hidropónicos in vitro.
Provedor de dados: 32
Autores: Jimarez Montiel, María Josefina.
Una alternativa para mejorar la producción de plantas de chile habanero es el cultivo de tejidos; no obstante, las características de esta técnica, incrementan considerablemente el costo de las plantas regeneradas y limita su capacidad de adaptación a las condiciones de invernadero o campo. El objetivo de este trabajo fue evaluar el efecto de mezclas de Perlita-Vermiculita (PV), Vermiculita-Perlita (VP), Fibra de coco-Tezontle (FT) y Perlita-Fibra de coco (PF), distintas concentraciones de los componentes del medio de cultivo (sales basales Murashige y Skoog (MS) y la solución nutritiva de Arnon y Hoagland y sacarosa), en la regeneración in vitro de plantas de chile habanero a partir de explantes de embriones e hipocótilos. Los explantes de embrión e...
Palavras-chave: Soluciones nutritivas; Sustratos; Embrión; Hipocótilo; Costos; Nutrient solutions; Substrates; Explants; Embryo; Hypocotyl; Producción de Semillas; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2302

Estudios anatómicos fisiológicos y nutrimentales de semillas de heliconias
Provedor de dados: 32
Autores: Benítez Domínguez, Lizeth.
Las heliconias son especies que desempeñan importantes funciones en los ecosistemas y que tienen un valor comercial alto debido a sus vistosas inflorescencias. Estas especies normalmente se reproducen de manera asexual y su producción se reduce a manejos tradicionales, por lo que existen pocos estudios de sus semillas y del manejo nutrimental de las plantas para elevar su producción. En esta investigación de hicieron estudios anatómicos, fisiológico y nutrimentales de cinco especies de heliconias: Heliconia bourgaeana, H. collinsiana, H. latispatha, H. psittacorum y H. rostrata. Las semillas de estas especies resultaron impermeables al agua. En H. collinsiana y H. latispatha los embriones se encontraron bien desarrollados y sin daños después de un año de...
Palavras-chave: Heliconiaceae; Embrión; Endospermo; Ácido abscísico; Nutrimentos; Heliconiaceae; Embryo; Endosperm; Abscisic acid; Nutrients; Maestría; Edafología.
Ano: 2010
URL: http://hdl.handle.net/10521/80

Genetic components affecting embryonic developmental time of Drosophila melanogaster
Provedor de dados: 74
Autores: Nascimento,Jurema Cruz do; Cruz,Ivana Beatrice Mânica da; Monjeló,Luiz Alberto; Oliveira,Alice Kalisz de.
The developmental time of the embryonic stage of Drosophila melanogaster was 21.66% faster and 14.75% slower than controls in populations selected for fast and slow developmental speed, respectively. The genetic model with two main loci with dominant and additive effect added to maternal effect and their relevant interactions can explain 96% of the phenotypic variability in the embryonic developmental time according to 14 crossing progenies involving fast and slow flies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Developmental rate; Embryo; Drosophila.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200008

Temperature and the progeny sex-ratio in Sciara ocellaris (Diptera, Sciaridae)
Provedor de dados: 74
Autores: Nigro,Rogério G.; Campos,Maria Cristina C.; Perondini,André Luiz P.
We found that the sex-ratio of an amphigenic strain of Sciara ocellaris varied widely from progenies with few males to progenies containing a larger proportion of males, with single-sex progenies being rare. The sex-ratio distributions were dependent on the temperature at which the stocks of flies were raised, with the sex-ratio distributions being symmetrical (i.e. about 50% males) at 18 °C and 20 °C while at the higher temperatures of 24 °C and 28 °C the distributions were skewed toward a high proportion of females with the mean proportion of males decreasing to about 30-37% per progeny. Temperature-shift experiments showed that high temperatures were effective only during the last stages of female pupal development plus a period after adult emergence,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex determination; Chromosome elimination; Embryo; Insect.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100026

Expression of Msx1 and Dlx1 during Dumbo rat head development: correlation with morphological features
Provedor de dados: 74
Autores: Katerji,Suhair; Vanmuylder,Nathalie; Svoboda,Michal; Rooze,Marcel; Louryan,Stéphane.
The Dumbo rat possesses some characteristics that evoke several human syndromes, such as Treacher-Collins: shortness of the maxillary, zygomatic and mandibular bones, and low position of the ears. Knowing that many homeobox genes are candidates in craniofacial development, we investigated the involvement of the Msx1 and Dlx1 genes in the Dumbo phenotype with the aim of understanding their possible role in abnormal craniofacial morphogenesis and examining the possibility of using Dumbo rat as an experimental model for understanding abnormal craniofacial development. We studied the expression of these genes during craniofacial morphogenesis by RT-PCR method. We used Dumbo embryos at E12 and E14 and included the Wistar strain as a control. Semi-quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dumbo rat; Msx1; Dlx1; Face; Embryo; Development.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200030

Species-specific differences in diatom-induced anomalies in calanoid copepod embryogenesis: are they linked to the stock-piled antioxidants?
Provedor de dados: 13
Autores: Khanaychenko, A. N.
Egg production and viability of co-occurring in nature copepods, Calanus helgolandicus and Calanoides carinatus, fed diatom, Thalassiosira rotula, and then switched to dinoflagellate Prorocentrum minimum, were compared in experimental conditions. Egg production of both species on diatom diet was similar (up to 17-22 eggs.female-1.d-1) but no viable nauplii were observed. Development was arrested at different stages depending on degree of anomalies. Abnormal embryos displayed various degrees of deteriorations in pigment distribution and organization of extracellular matrix. Reproductive responses of C.carinatus both to negative (diatom) and positive (dinoflagellate) diets were postponed in comparison with quick responses of C.helgolandicus. After 3 days of...
Tipo: Journal Contribution
Palavras-chave: Calanoid copepods; Diatom; Embryo; Anomaly; Extracellular matrix; Pigment; Каланоидные копеподы; Диатомовые; Эмбрион; Аномалии; Экстраклеточный матрикс; Пигмент Diatoms Embryos Pigments Pigments Diatoms http://aims.fao.org/aos/agrovoc/c_5875 http://aims.fao.org/aos/agrovoc/c_32657.
Ano: 2004
URL: http://repository.ibss.org.ua/dspace/handle/99011/92

Morphological development of Corydoras aff. paleatus (Siluriformes, Callichthyidae) and correlation with the emergence of motor and social behaviors
Provedor de dados: 76
Autores: Rodríguez-Ithurralde,Daniel; del Puerto,Gabriela; Fernández-Bornia,Fernando.
Here we examine major anatomical characteristics of Corydoras aff. paleatus (Jenyns, 1842) post-hatching development, in parallel with its neurobehavioral evolution. Eleutheroembryonic phase, 4.3-8.8 days post-fertilization (dpf); 4.3-6.4 mm standard length (SL) encompasses from hatching to transition to exogenous feeding. Protopterygiolarval phase (8.9-10.9 dpf; 6.5-6.7 mm SL) goes from feeding transition to the commencement of unpaired fin differentiation, which marks the start of pterygiolarval phase (11-33 dpf; 6.8-10.7 mm SL) defined by appearance of lepidotrichia in the dorsal part of the median finfold. This phase ends with the full detachment and differentiation of unpaired fins, events signaling the commencement of the juvenile period (34-60 dpf;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catfish; Developmental neuroethology; Embryo; Larvae; Teleost.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212014000200009

Differential bioaccumulation behaviour of Ag and Cd during the early development of the cuttlefish Sepia officinalis
Provedor de dados: 5
Autores: Lacoue Labarthe, T; Warnau, A; Oberhansli, F; Teyssie, J; Koueta, Noussithe; Bustamante, Paco.
Cuttlefish eggs were exposed to background concentrations of dissolved Ag and Cd, using the radiotracers Ag-110m and Cd-109. At different time of the embryonic development (50 days), some eggs were placed in non-contaminating conditions. During the experiment, the uptake and depuration kinetics, and distribution of these metals among the egg compartments (i.e. eggshell, vitellus, peri-vitelline fluid and embryo) were assessed. In parallel, experiments were conducted with sub-lethal concentrations of stable Ag and Cd (2 and 1 mu g l(-1), respectively) to compare the metal behaviour at higher concentrations. From the spawning date up to I month of development, both metals were taken up efficiently by the eggs, reaching load/concentration ratio (LCR) of 1059...
Tipo: Text
Palavras-chave: Cephalopod; Permeability; Eggshell; Embryo; Biokinetics; Silver; Cadmium.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3882.pdf

Effects of dissolved mercury on embryogenesis, survival, growth and metamorphosis of Crassostrea gigas oyster larvae
Provedor de dados: 5
Autores: Beiras, Ricardo; His, Edouard.
The effects of mercury (Hg) concentrations ranging from 0 (control) to 1024 µg 1-1 upon embryogenesis, survival, growth and metamorphosis of Crassostrea gigas (Thunberg) oyster larvae were investigated. Embryogenesis was abnormal in 50 % of the individuals at 11 µg 1-1 . The 48 h LD50 for D·shaped, umbonate and pediveliger larvae were 33, 115 and 200 µg 1-1 respectively. The increase in LD50 was partially explained by the larval weight increase, although weight-specific tolerance to Hg was higher in smaller larvae. Growth, the most sensitive physiological process studied, was significantly retarded at 4 µg 1-1. The metamorphosis rate was significantly reduced when competent pediveligers were exposed to 64 µg 1-1 for 48 h prior to the addition of the...
Tipo: Text
Palavras-chave: Mercury; Oyster; Larvae; Embryo; Bioassay; Ecotoxicology; Crassostrea gigas.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00037/14867/12191.pdf

Effects of dissolved mercury on embryogenesis, survival and growth of Mytilus galloprovincialis mussel larvae
Provedor de dados: 5
Autores: Beiras, R; His, Edouard.
The effects of Hg concentrations ranging from 0 (control) to 1024 mu g l(-1) upon embryogenesis, survival and growth of Mytilus galloprovincialis mussel larvae were investigated. Embryogenesis was abnormal in 50% of the individuals at 10 mu g l(-1). The 48 h LC(50) for D-shaped, early umbonate, late umbonate and eyed larvae were 51, 164, 322 and 383 pg l(-1) respectively. The LC(50) was an allometric function of ash-free dry weight with exponent b = 0.60. Larval growth was significantly reduced after 6 d exposure to 4 mu g Hg l(-1) and after 10 d exposure to 2 mu g l(-1). No significant differences in lethal or sublethal sensitivity to Hg were found between M. galloprovincialis and previously studied Crassostrea gigas embryos and larvae. The implications...
Tipo: Text
Palavras-chave: Mercury; Mytilus; Mussel; Larva; Growth; Embryo; Bioassay; Ecotoxicology.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00258/36946/35665.pdf

Influence of environmental conditions on early development of the hydrothermal vent polychaete Alvinella pompejana
Provedor de dados: 5
Autores: Pradillon, F; Le Bris, Nadine; Shillito, B; Young, C; Gaill, F.
Dispersal and colonisation processes at deep-sea vents are still not fully understood, essentially because early life stages of vent species remain unknown. The polychaete worm Alvinella pompejana forms colonies on chimney walls at East Pacific Rise vent sites where the temperature can frequently exceed 20 degrees C. In vitro studies in pressure vessels showed that the early embryos tolerate temperatures in a lower range (10-14 degrees C), suggesting that they would have to escape the colony to develop. Pressure vessels offer the advantage that each parameter can be independently controlled, but they do not simulate the more complex and dynamic conditions naturally encountered at vent sites. Accordingly, in addition to incubations in pressure vessels, we...
Tipo: Text
Palavras-chave: Environmental condition; H2S; Temperature tolerance; Polychaete; Pressure vessel; Embryo; Development; Hydrothermal vent.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-600.pdf

Review of the reproductive physiology of the scallop, Pecten maximus, applicable to intensive aquaculture
Provedor de dados: 5
Autores: Devauchelle, Nicole; Mingant, Christian.
This paper presents a summary of information on reproduction biology in the scallop, Pecten maximus, which is required for the successful reseeding and intensive aquaculture programs developed in France since 1982. Data are presented on the following subjects: environmental factors that sustain gametogenic activity and the time of year this activity occurs; biochemical changes that are associated with gametogenesis; the consistency of egg quality; and development of a simple test to predict egg quality.
Tipo: Text
Palavras-chave: Aquaculture; Pecten maximus; Ecloserie; Physiologie de la reproduction; Embryon; Gamète; Aquaculture; Pecten maximus; Scallop; Hatchery; Reproductive physiology; Egg quality; Embryo; Gamete.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00038/14890/12216.pdf

Setting of a procedure for experimental incubation of Pacific oyster (Crassostrea gigas) embryos
Provedor de dados: 5
Autores: Suquet, Marc; Amourda, Christopher; Mingant, Christian; Queau, Isabelle; Lebrun, Luc; Brizard, Raphael.
Standardized experimental protocols designed to study Pacific oyster (Crassostrea gigas) gamete quality have not been previously published. Gamete sampling variations and confounding effect of interacting factors result in large variations between replicates, decreasing the effects of the studied factors. This work aims at defining a standardized procedure for incubation of oyster embryos designed for experimental purposes.   In a first phase, four experiments were developed to improve embryo sampling and handling. They showed that a minimum of 50 embryos must be counted to decrease the variation between counting replicates. For reliable results, sampling must be carried out from 0 to 7 min after a careful agitation of seawater containing embryos...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Experimental; Embryo; Incubation.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3593.pdf

Embryonic Development of Aplodinotus grunniens (Perciforme: Sciaenidae) in Tenosique, Tabasco, Mexico
Provedor de dados: 77
Autores: Hernández-Gómez,Raúl E; Perera-Garcia,Martha A; Valenzuela C,Ignacio; Duran M,Temani; Mendoza-Carranza,Manuel.
The description of the embryonic and larval development was based on 30 eggs and 30 larvae. Breeders were collected on the Usumacinta River, Tabasco, Mexico and were subjected to manual fertilization in May 2011. Fertilized eggs were spherical, pelagic, translucent and non-adhesive and had an average diameter of 1.07 mm (SD = 0.10), showing a concentric lipid droplet of 0.85 mm (SD = 0.02). The embryo occupied 100% of the perivitelline space at hour 14:08 post-fertilization and the egg measured 1.18 mm (SD = 0.14) at this stage. Eclosion started 17 hours post-fertilization when larvae had a length of 2.54 mm (SD = 0.66). Eight preanal and 13 postanal myomeres were observed exhibiting a light brown color. The morphologic characters recorded in this study...
Tipo: Journal article
Palavras-chave: Embryo; Drum; Aplodinotus grunniens; Usumacinta.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200047

Histochemical Study of Early Embryo Implantation in Rats
Provedor de dados: 77
Autores: Spósito,Danilo Rodrigues; Santos Jr,Arnaldo Rodrigues.
Embryo implantation is the process that results in attachment of the conceptus to the uterine wall. In this histochemical study, we investigated the early stage of embryo implantation in rats by morphological analysis and by the detection of total proteins and glycosaminoglycans using hematoxylin-eosin, toluidine blue at pH 4.0 (TB), and Xylidine Ponceau at pH 2.5 (XP). In non-pregnant females, the uterine layers could be clearly distinguished and showed the normal histology of the organ. In pregnant females, an increase in the number of cells and a reduction in the interstitial space were observed in the endometrium close to the implantation sites. The blastocyst was partially inserted in the endometrium, with the observation of the inner cells mass...
Tipo: Journal article
Palavras-chave: Implantation window; Endometrium; Trophoblast; Embryo.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100032

Co-culture of Mouse Two-cell Embryos on Human Endometrial Stromal Cells in Proliferative and Secretory Phases
Provedor de dados: 77
Autores: Fakheri,Taravat; Nankali,Anisodowleh; Khazaei,Mozafar; Ghanbari,Ali; Abedini,Mehrandokht; Saeidiborojeni,Hamidreza.
There were no significant differences in the distribution of embryos reaching to 2- cells, 4- cells, morula or blastocysts culturing on human endometrial stromal cells (Secretory or proliferative phases). The percent of morula in stage A (without fragmentation), stage B (&lt;25% fragmentation), stage C (25-50% fragmentation) and stage D (&amp;gt;50% fragmentation) and did not showed significant differences between two coculture groups. Thus, the phase that the endometrial stromal cells were in thereby did not affect on the quality of embryos.
Tipo: Journal article
Palavras-chave: Co-culture; Embryo; Endometrium; Mice; Proliferative phase; Secretory phase.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400014

Evaluation of Placental Epigenetic Changes due to Morphine Consumption
Provedor de dados: 77
Autores: Kazemi,Masoomeh; Tekieh,Elaheh; Golabi,Sahar; Sahraei,Hedayat.
Based on previous studies, a variety of bioenvironmental elements including inappropriate nutrition, diseases, infections, stressors, and medications are involved in epigenetic changes. Drug abuse is one of the most important causes of epigenetic changes and a concern in today's world. Studies have shown that morphine use by pregnant mothers causes several disorders in mothers in addition to transferring abnormalities to the next generation (placenta and embryo). Epigenetic factors such as morphine cause changes in gene expression in placenta as the first embryonic defense barrier. Because placenta does all the nutritional exchanges between mother's and embryo's blood, placental health guarantees normal embryonic development. Many studies have been...
Tipo: Journal article
Palavras-chave: Development; Morphine; Embryo; Epigenetic; Pregnancy; Placenta.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100036

Effect of Preconception Gamma Irradiation on Morphometric Assessment of Adult Female Mice and Embryo
Provedor de dados: 77
Autores: Edagha,Innocent A; Shokunbi,Matthew T; Adenipekun,Adeniyi A; Bassey,Rosemary B; Ekong,Moses B.
The aim was to study the effect of preconception gamma irradiation on the gross morphometry of the adult female mice and its embryo. Twenty-seven mice; 18 females and 9 males: subdivided into 3 groups namely (Control, Non-Irradiation and Radiation) containing 6 females and 3 male mice each in 2:1 ratio. A gamma irradiation dose of 1Gy/min was delivered to each batch of mice exposed by a Cobalt 60, Theratron 780c model, by Atomic Energy of Canada Limited (AECL) at the Radiotherapy department of the University College Hospital, Ibadan. All the animals were mated 1 week post irradiation. Vaginal plugs were confirmed, and the pregnant females were sacrificed on day 14 of gestation by chloroform inhalation. The gross morphology of the female mice and their...
Tipo: Journal article
Palavras-chave: Preconception; Irradiation; Gamma ray; Spinal cord; Embryo.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200039

Bovine Oocytes and Early Embryos Express mRNA Encoding Glycerol Kinase but Addition of Glycerol to the Culture Media Interferes with Oocyte Maturation
Provedor de dados: 19
Autores: OKAWARA, Sumika; HAMANO, Seizo; TETSUKA, Masafumi; 手塚, 雅文.
Glycerol plays multi-functional roles in cellular physiology. Other than forming the backbone molecule for glycerophospholipid and triglyceride (TG), glycerol acts as an energy substrate for glycolysis. Spermatozoa are known to utilize glycerol for energy production, but there are no reports of this in oocytes. In this study, the value of glycerol as an energy substrate for bovine oocyte maturation (Exp. 1) and the gene expression of glycerol kinase (GK), an enzyme crucial for cellular glycerol utilization, in bovine oocytes and early embryos (Exp. 2) were examined. In Exp. 1, in vitro maturation (IVM) was conducted using synthetic oviduct fluid supplemented with/without glucose (1.5 mM) and/or glycerol (1.0 mM), and maturation rate, degree of cumulus...
Palavras-chave: Bovine; Embryo; Glycerol; Glycerol kinase (GK); Oocyte maturation.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2829

Preimplantation Development of Embryos in Labrador Retrievers
Provedor de dados: 19
Autores: ABE, Yasuyuki; SUWA, Yoshinori; YANAGIMOTO-UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志.
Preimplantation development of canine embryos is not well understood. To understand the timing of preattachment embryogenesis relative to the luteinizing hormone (LH) surge, early embryonic development was examined in Labrador Retrievers after artificial insemination. The embryos migrated from the oviduct to the uterus beginning on day 11 after the LH surge. This transport must be completed within 24 h. By day 13 after the LH surge, all of the embryos had moved and were localized in the uterus. The embryos developed to the morula stage within 11-13 days and to the blastocyst stage within 14 days after the LH surge, respectively. These findings add to the current understanding concerning the physiology of preimplantation development and should help further...
Palavras-chave: Dog; Embryo; Preimplantation development.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3029

Bovine embryo induces an anti-inflammatory response in uterine epithelial cells and immune cells in vitro: Possible involvement of interferon tau as an intermediator
Provedor de dados: 19
Autores: Talukder, Anup K.; Yousef, Mohamed S.; Rashid, Mohammad B.; Awai, Kensuke; Acosta, Tomas J.; Shimizu, Takashi; Okuda, Kiyoshi; Shimada, Masayuki; Imakawa, Kazuhiko; Miyamoto, Akio.
Recent observations suggest that the bovine uterus starts to react to the early embryo immediately after its arrival from the oviduct. The present study aimed to investigate the effect of the early developing embryo on the immune-related gene profile in bovine uterine epithelial cells (BUECs) in vitro, and to further examine the impact of conditioned media (CM), either from embryo-BUEC co-culture or embryo culture alone, on gene expression in peripheral blood mononuclear cells (PBMCs). First, BUECs were co-cultured with morulae (n = 10) for D5-D9 (D0 = IVF), and gene expression in BUECs was analyzed. Subsequently, PBMCs were cultured in CM from embryo-BUEC co-culture or D5-D9 embryo culture, and gene expression was evaluated. In BUECs, the embryo induced...
Palavras-chave: Bovine; Embryo; Immunity; Interferon tau (IFNT); Uterus.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4532

Variation on embryo development of Ilex paraguariensis Seeds
Provedor de dados: 190
Autores: Fowler, João Antonio Pereira; Sturion, José Alfredo; Zuffellato-Ribas, Katia Christina.
Ilex paraguariensis St.Hil., popularly named erva-mate in Brazil, is an indigenous species of the Araucaria Forest with a large geographic distribution and a high social and economic importance. The seeds of this species exhibits physiologic and seed coat  impermeability dormancies and needs stratification on sand in order to break them. This work aimed to evaluate the influence of genotype -environment interaction on embryo development of erva-mate seeds formed under different environmental conditions. The fruits were collected in Cascavel, Toledo and Campo Mourão, State of Paraná, and in Soledade, State of Rio Grande do Sul, in February 2003. The experiment was carried out on Forest Seed Laboratory of Embrapa Florestas, in a completely randomized design...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis; Embryo; Germination; Dormancy. Embrião; Germinação; Dormência; Erva-mate.
Ano: 2010
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/134

Morphology and anatomy of the developing fruit of Maclura tinctoria, Moraceae
Provedor de dados: 88
Autores: Oyama,Sayuri de Oliveira; Souza,Luiz Antonio de.
(Morphology and anatomy of the developing fruit of Maclura tinctoria, Moraceae). Maclura tinctoria (L.) D. Don ex Steudel was selected for the present study due of its economic and medicinal importance. The purpose of this investigation is to present a detailed description of the fruit development, specially by: (a) defining the fruit type presented by the species, and (b) characterizing the seed type of the species based upon the presence or not of mechanical tissue on the seed-coat. The fruit originates from the subglobose female inflorescence which consists of small unipistillate flowers with superior ovary, unilocular and uniovular apical placentation. The mature fruit is multiple, constituted of small drupes. The ovule is ana-campylotropous,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drupe; Embryo; Integument; Pericarp; Perigone.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042011000200006

Viability and vigor of jamun (Syzygium cumini) seeds
Provedor de dados: 88
Autores: Sivasubramaniam,K.; Selvarani,K.
Jamun (Syzygium cumini L. Skeels) (Black plum, Damson plum) fruits weigh between 2-5 g at maturity. Fresh seeds represented 20-80% of the total fruit weight; the seed coat and cotyledons contributed 6% and 94% to the total seed weight respectively, while the weight of the embryonic axis was insignificant. Only the embryonic axis stained with Tetrazolium, not the cotyledons. The seeds are polyembryonic with up to four embryos, of which at most three germinate. Decoated seeds germinated faster than coated seeds under nursery conditions, with high significant germination percentages, dry matter production rates and vigor indices. The lack of staining of the cotyledon by tetrazolium was probably due to the presence of an impermeable layer. Decoating seeds for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Decoated seeds; Embryo; Myrtaceae; Polyembryony; Tetrazolium.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000400012

Effect of breeder age on eggshell thickness, surface temperature, hatchability and chick weigh
Provedor de dados: 89
Autores: Gualhanone,A; Furlan,RL; Fernandez-Alarcon,MF; Macari,M.
Two experiments were carried out to study the effect of breeder age on incubation parameters (hatchability, eggshell thickness, egg surface temperature and chick weight). In Exp. 1, fertile eggs (30- and 60-wk-old breeders) were incubated at three different temperatures (36.8, 37.8 and 38.8 ºC). Eggshell surface temperature was measured by attaching a thermocouple to the shell and data were collected in a datalogger every ten minutes. This study was conducted according to a 3 x 2 factorial design (three temperatures and two breeder ages). Data revealed that eggshell surface temperature changed according to incubation temperature, with the main increase occurring between 10 and 13 days of incubation, and that the maximum increase in eggshell surface...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler breeder; Development; Embryo; Temperature; Incubation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000100002

Effect of broiler breeder age on pancreas enzymes activity and digestive tract weight of embryos and chicks
Provedor de dados: 89
Autores: Maiorka,A; Santin,E; Silva,AVF; Routman,KS; Pizauro Jr.,JM; Macari,M.
A study was carried out to evaluate the effect of broiler breeder age on the development of the digestive tract of embryos and chicks. Fertilized eggs Cobb from 30 and 60 week-old broiler breeder was utilized in this experiment. The results showed that eggs from older (60 weeks of age) broiler breeders were heavier (p = 0.001) than those from younger (30 weeks of age) broiler breeder. In addition, older broiler breeder had larger (p = 0.001) embryos showing a higher yolk sac (p = 0.001) and higher gastrointestinal tract relative weight (p = 0.007) than those from younger broiler breeder. The activities of pancreatic lipase and trypsin enzymes were also higher in embryos from older broiler breeder than those from younger broiler breeder (p = 0.001 and p =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler breeder age; Embryo; Enzymatic activity; Gastrointestinal tract.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100003

Storage Period Affects Weight Loss of Japanese Quail Eggs
Provedor de dados: 89
Autores: Roriz,BC; Sgavioli,S; Garcia,RG; Nääs,IA; Domingues,CHF; Caldara,FR; Rombola,LG; Ayla,CM; Bernnecke,K.
ABSTRACT Long storage periods may increase embryo mortality. The objective of this study was to evaluate the effects of storage period on the weight loss, eggshell surface temperature, hatchability, and embryonic mortality of Japanese quail eggs. Two hundred fertile eggs were collected from a flock of 30-week-old Japanese quails (Coturnix coturnix japonica). The eggs were collected for 10 consecutive days after lay, and immediately incubated. A completely randomized experimental design with 10 treatments, corresponding to the number of days eggs were stored between egg collection and setting, with 20 replicates each, was applied. Egg weight loss increased with storage period duration, starting on day 6 (2.1%, on average) and reached 3.26%, on average, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Hatchability; Incubation; Laying; Temperature.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400589

Effect of Broiler Breeder Age on the Intestinal Mucosa Development of the Embryos at 20 Days of Incubation
Provedor de dados: 89
Autores: Maiorka,A; Silva,AVF; Santin,E; Bruno,LDG; Boleli,IC; Macari,M.
ABSTRACT This study investigated the effect of broiler breeder age on the morphological development of the small intestine broiler embryos (villus height, crypt depth, microvillus height, and villus density) at 20 day of incubation. Eggs obtained from 30- and 60-wk-old broiler breeders were used. The results showed that embryos from older broiler breeders presented longer villi in the duodenum, jejunum, and ileum compared with younger broiler breeders. In addition, embryos from older broiler breeders presented deeper crypts in the jejunum and ileum, longer microvilli in jejunal enterocytes, and lower villus density (microvillus number/mm2) in the duodenum and ileum than younger breeders. These results suggest that breeder age influences the gut mucosa...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler breeder age; Embryo; Microvillus; Small intestine; Villus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600079

Femur Properties of Embryo in the Layer Hybrid and Pure Breeds
Provedor de dados: 89
Autores: Onbaşılar,EE; Güngör,ÖF; Demir,T; Erbay,FK Elibol; Kahraman,M; Taban,S; Çalık,A; Pirpanahi,M.
ABSTRACT Layer hybrids are more superior in the egg yield than pure breeds. But this superiority causes some problems in the bones during the production period. There are a lot of differences among genotypes in the production period that have been examined extensively, however, the differences throughout incubation period aren’t known yet. Thus, the objective of this study was to evaluate the differences in femur properties of pure breed and layer hybrid embryos during the incubation period. A total of 354 fresh hatching eggs were used from one hybrid (Lohman White) and two pure breeds (Denizli &amp; Gerze). The eggs were incubated. Hatching eggs from each genotype at the beginning of embryonic days (E) 19 and E21 were examined. At these embryonic ages, 12...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Layer; Pure breed; Hybrid; Embryo; Femur.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400805

In ovo supplementation of 25(OH)D3 to broiler embryos
Provedor de dados: 89
Autores: Gonzales,E; Cruz,CP; Leandro,NSM; Stringhini,JH; Brito,AB.
A dose of 0.3 mL of water solution containing 0.00 (control), 0.625, 1.250 or 1.875 µg of 25-hydroxy cholecalciferol (25(OH)D3) was administered to 312 fertile eggs derived from 49-w-old Cobb 500 broiler breeders on the 17th day of incubation (DE17) via allantoic cavity. After treatment, eggs were distributed and maintained until hatching in four incubators set at 37.8 ºC and 55% RH. Each incubator received eggs from all treatments, according to a block design with four treatments of 77-79 replicates each. Hatching was checked every two hours from 484h to 512h of incubation to evaluate productivity and chick qualities. Chicks were housed until 10 days of age in heated battery cages according to a block design with four treatments of 10 replicates of six...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler; Cholecalciferol; Embryo; In-ovo supplementation; Vitamin D.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000300005

Effect of Incubator Type and Broiler Breeder Age on Hatchability and Chick Quality
Provedor de dados: 89
Autores: Araújo,ICS; Leandro,NSM; Mesquita,MA; Café,MB; Mello,HHC; Gonzales,E.
ABSTRACT Two experiments were conducted to evaluate the effect of broiler breeder age and incubator type on hatching parameters, hatch window, embryo diagnosis results, and hatchling physical quality. The treatments consisted of a combination of three broiler breeder ages (29, 35 and 59 weeks of age) and two incubator types (single stage, SS; or and multiple stage, MS). A completely randomized design in a 3x2 factorial arrangement was applied. In Experiment I, 1,896 eggs were used and 360 eggs in Experiment II. There was an interaction between breeder age and incubator type only for hatchling physical quality score. Independently of incubator type, hatchability rate, late embryo mortality, and egg contamination were higher in the eggs laid by older...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Hatch window; Multiple-stage and single-stage incubation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600017

Comparison of Some Relative Gene Expressions in the Yolk Sac Membrane and Small Intestine of the Embryos in the Layer Hybrid and Pure Breeds
Provedor de dados: 89
Autores: Onbaşilar,EE; Erdoğan,M; Erol,S; Kahraman,M; Güngör,ÖF.
ABSTRACT Pure breeds are important due to the fact that they adapt well to poor husbandry conditions. However, gene expression of digestive enzymes and nutrient transporters of pure breeds and layer hybrids have not been studied yet. Therefore, this study was directed to detect the gene expression of digestive enzymes and nutrient transporters in the yolk sac membrane and small intestine of the embryos in layer hybrid and pure breeds. A total of 354 hatching eggs were collected from 29 wk old Denizli, Gerze and Lohman White (LW) breeder flocks. Twelve eggs from each genotype were selected at embryonic day 19 (E19) and day 21 (E21). APN, SI, PepT1, EAAT3, CAT1, B0AT, SGLT1 and GLUT5 mRNA expressions were evaluated in the yolk sac membrane (YSM) and small...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Gene expression; Layer hybrid; Pure breed.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300435

Identification of plantlet genetic origin in polyembryonic mango (Mangifera indica, L.) cv. Rosinha seeds using RAPD markers
Provedor de dados: 94
Autores: Cordeiro,Maria Cristina Rocha; Pinto,Alberto Carlos Queiróz; Ramos,Víctor Hugo Vargas; Faleiro,Fábio Gelape; Fraga,Lilia Marta Santos.
Mango can be propagated by seeds or by grafting. For commercial purpose, grafting is the most appropriate method because it maintains the genetic characters from the propagated variety. To obtain grafted mango it is important to use polyembryonic varieties as rootstock since they produce a zygotic and many nucellar plantlets. The nucellar plantlets maintain the genetics of the mother-plant thus, are preferred for grafting since they supposedly give more uniformity to the orchard. In general, nurserymen use the most vigorous plantelet to graft, believing that they are nucellar. But, orchard disuniformities on height and yield are very common among mango trees of commercial orchards in Northeast region. The objective of this paper was to identify the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plantlet identification; Embryo; Rootstock; Orchard uniformity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300025

CHEMICAL COMPOSITION AND TETRAZOLIUM TEST OF Syagrus romanzoffiana (Cham.) Glassman SEEDS
Provedor de dados: 94
Autores: IOSSI,EMERSON; MORO,FABIOLA VITTI; VIEIRA,BRUNO GUILHERME TORRES LICURSI; BARBOSA,RAFAEL MARANI; VIEIRA,ROBERVAL DAITON.
ABSTRACT  Queen palm is a tropical species of ecological importance and economic potential in various uses, including food. However, the knowledge about the nutritional value of fruits and the estimation of seed viability are still scarce. The aims of this work were to study the biometrics and chemical composition of fruits and seeds; describe the anatomy of embryos and assess the viability of the tetrazolium test. Mature fruits were collected, processed and submitted to biometry, water content, pulp and seeds chemical composition, germination and emergence in the laboratory evaluations. For the tetrazolium test, whole embryos were immersed in solution at 40 °C for one, two, four and six hours and compared with germination. The fruit pulp is rich in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Queen palm; Embryo; Germination; Palm; Pyrene.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000402001

Relationship between eggplant seed morphology and germination
Provedor de dados: 95
Autores: Silva,Vanessa Neumann; Cicero,Silvio Moure; Bennett,Mark.
Structural differences such as abnormalities, damage and free spaces in seeds may affect germination. The aim of this study was to study the relationship between eggplant seed morphology and seed germination. Ten seed lots of the eggplant cultivar Embu were evaluated by X-ray image analysis and the germination test. Seed image analysis was performed by Image Pro Plus® software and the whole seed area and free space between the embryo and endosperm were measured. The internal seed area filled by the embryo and endosperm was calculated from the difference between the whole seed and free space areas. Based on these results and visual seed analysis, seeds were classified into three categories and information on germination was obtained for each one. X-ray...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: X-ray; Solanum melongena L.; Embryo.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000400010

Embryo development and follicular status of Toggenburg does fed urea diet
Provedor de dados: 96
Autores: Amorim,Lincoln da Silva; Torres,Ciro Alexandre Alves; Siqueira,Luiz Gustavo Bruno; Fonseca,Jeferson Ferreira da; Guimarães,José Domingos; Carvalho,Giovanni Ribeiro de; Alves,Nadja Gomes; Oliveira,Margarida Maria Nascimento Figueiredo de.
The effects of feeding urea on embryo production, quality and developmental stages and follicular status at the beginning of a superovulatory treatment of Toggenburg does fed urea diet were investigated in this study. Eighteen females were randomly allocated to receive diets with: no urea (control, n=8), and 2.4% urea dietary dry matter (n=10) UDM. The embryo recovery was performed at day 7 or 8 of estrus cycle by transcervical technique and classified according to quality and developmental stage. The follicular status was determined by transrectal ultrasonography at the first day of FSH injection. The number and quality of embryos were not affected by dietary urea concentration. Embryos from does treated with 2.4% UDM were recovered at advanced stage....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Follicle; Goat; Superovulation; Urea.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000200007

Macroscopic embryonic development of Guinea fowl compared to other domestic bird species
Provedor de dados: 96
Autores: Araújo,Itallo Conrado Sousa de; Lucas,Luana Rudrigues; Machado,Juliana Pinto; Mesquita,Mariana Alves.
ABSTRACT Since few studies have addressed the embryonic development of Guinea fowl (Numida meleagris), the objective of the present study was to evaluate its embryonic development in the Cerrado region of Brazil and compare the results to published descriptions of the embryonic development of other domestic bird species. The commercialized weight for Guinea fowl eggs used in the experiment was found to be 37.57 g, while egg fertility was 92%. Embryo growth rate (%) was higher on the sixth day of incubation relative to other days. The heart began beating on the third day of development, while eye pigmentation and upper and lower limb buds appeared on the sixth day. The yolk sac was incorporated on the 24th day followed by chick external pipping and hatching...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Fertile eggs; Gallus gallus domesticus; Numida meleagris.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100604

In vitro embryo production in small ruminants
Provedor de dados: 96
Autores: Freitas,Vicente José de Figueirêdo; Melo,Luciana Magalhães.
This paper reviews the technical bases of in vitro embryo production in small ruminants with special attention to the results obtained by our group in Northeastern Brazil. The laparoscopic oocyte recovery in hormonally treated live animals indicates a promising future for the application of this technique to genetic improvement program. New molecular biology tools should provide information to improve the efficiency of in vitro maturation. Furthers efforts have to be made to improve the oocyte maturation and to standardize the semen-capacitating process.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Goat; In vitro production; Sheep.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300045

Oocyte development in cattle: physiological and genetic aspects
Provedor de dados: 96
Autores: Britt,Jack H.
Oocytes in cattle are formed during embryogenesis and develop within individual follicles in the cortex of the ovary. Dormant primordial follicles become active and undergo progressive development at regular intervals commencing during the late fetal stage and continuing throughout adulthood. Once activated, follicles and oocytes in a cohort either grow to maturation and ovulation or undergo atresia, ultimately depleting the ovaries of germ cells. It takes an estimated 100 days for a follicle and its oocyte to reach the mature ovulatory stage. Within an individual, number of follicles in one ovary is similar to number in the other ovary; however, there are large differences among individuals in total number of follicles present in both ovaries. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Follicle; Ovary; Oocyte; Embryo; Fecundity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300013

Association of recombinant bovine somatotropin (rBST) with equine chorionic gonadotropin (eCG) on antral follicle count and oocyte production in Holstein and Tabapuã heifers
Provedor de dados: 96
Autores: Morais,Hévea de; Spuri,Renata; Gonçalves,Tarcísio de Moraes; Carvalho,Rafaela Rodrigues de; Andrade,Renato Campos; Pinto,Tássia Louregiani Carvalho; Souza,José Camisão de.
The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2) before two treatments: stimulation (6 Holstein and 8 Tabapuã), 500 mg of rbST (Boostin®) on D0 and 500 IU of eCG (Folligon) on D2; and control (6 Holstein and 8 Tabapuã), in which heifers received injections of the excipient. Heifers were aspirated on D4. Oocytes were subjected to a well established commercial in vitro embryo production protocol (Vitrogen®) and embryos were evaluated seven days after fertilization. There was an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Hormonal stimulation; Ovary.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200004

การงอกและการเจริญเติบโตของเอมบริโอพืชในสกุล Prunus ที่ยังพัฒนาไม่เต็มที่โดยวิธีการเพาะเลี้ยงเอมบริโอ
Provedor de dados: 106
Autores: Nonglak Jeengool.
Thesis (M.Sc. in Agriculture)
Tipo: PhysicalObject
Palavras-chave: Peach; Prunus persica; Nectarine; Plum; Prunus domestica; Plant; Genus Prunus; Embryo; Germination; Growth; Embryo culture technique; Culture media; Woody plant medium; WPM; Brooks and Hough; BH; พีช; เนคทารีน; พลัม; พืช; เอมบริโอ; การงอก; การเจริญเติบโต; การเพาะเลี้ยงเอมบริโอ; อาหารเพาะเลี้ยง; สูตรอาหาร.
Ano: 2003
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5477

Light and electron microscopy study of late embryonic development in the land snail Limicolaria flammea (Müller) (Pulmonata, Achatinidae)
Provedor de dados: 104
Autores: Egonmwan,Rosemary I.
The late stages of embryogenesis in the achatinid land snail Limicolariaflammea (Müller, 1774) were described using light and electron microscopy. Embryos at various stages of development were present in the eggs during the first hour after they were laid, from 4-cell blastulae to morulae and fairly advanced stages. The advanced embryo which was fully developed on the second day bears a long cephalic sac, first to be developed, attached to the embryo and a podocyst which is attached to the foot of the embryo. Both of these structures are reduced in size as embryogenesis progresses until they finally disappear at about the 7th day after the egg was deposited. The embryonic shell was apparent on the second day and spiral coiling was apparent at about day 5....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cephalic sac; Embryo; Embryonic shell; Foot; Podocyst.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752007000200022

Embriogenesis and larval ontogeny of the "piau-gordura", Leporinus piau (Fowler) (Pisces, Anostomidae) after induced spawning
Provedor de dados: 104
Autores: Borçato,Fábio Luiz; Bazzoli,Nilo; Sato,Yoshimi.
Mature males and females of Leporinus piau (Fowler, 1941) were induced to reproduce through a hypophysation process. Extrusion occurred 12 h or 312 hours-degree after the hypophysation, at a water temperature of 26°C. Fertilized eggs were maintained in incubators at 24°C. Embryonic development, was evaluated using every 10 minutes, fresh egg samples which were analyzed under stereoscopic microscope. The larvae were collected at 24-hour intervals for seven days after hatching, fixed in Bouin's fluid and were submitted to routine histological techniques. The eggs of L. piau were slightly gray, non-adhesive and round-shaped. After 1.5 h the embryo was at the 64-blastomere phase and showed a wide yolk region on the vegetative pole. Within 6.25 h, blastopore...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Induced spawn; Teleost fish.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000100019

Taxonomic relevance of seed and seedling morphology in two Amazonian species of Entada (Leguminosae)
Provedor de dados: 36
Autores: Rodrigues,Rodrigo Schütz; Feitoza,Gildo Vieira; Flores,Andréia Silva.
We carried out a comparative morphological study to evaluate the taxonomic value of seed and seedling traits of Entada polystachya and E. simplicata (Leguminosae, Mimosoideae, Mimoseae). Seeds of both species were collected from wild populations in Roraima State, Brazil. Our results show consistent quantitative and qualitative differences between these species regarding their seeds and seedlings. Testa fracture lines, funiculus impression, lens characters, radicle shape and length, and cotyledon lobes length, seedling morphology group, number of pinnae at first node, and hypocotyl length permit a straightforward distinction of E. polystachya from E. simplicata, supporting a recent treatment of the latter taxon as a distinct species, endemic to Roraima...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fabaceae; Mimosoideae; Embryo; Post-seminal development; Testa.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672014000100003

Simultaneous dehydration and infiltration with (2-hydroxyethyl)-methacrylate (HEMA) for lipid preservation in plant tissues
Provedor de dados: 39
Autores: Oliveira,João Marcelo Santos de.
Although this study aims to develop an improved method for the preservation of reserve lipids in plant tissues for different uses in plant anatomy, it mostly aims to develop an improved method for the identification of lipid reserves where synthesis or storage occurs. The proposed procedures entail only the utilization of (2-hydroxyethyl)-methacrylate (HEMA) as a dehydration agent. One of the procedures is based on the gradual exchange of aqueous HEMA solutions with increasing concentrations. In another procedure, dehydration and infiltration are induced by the presence of silica gel around a modified microcentrifuge tube containing the aqueous HEMA solution with the plant tissues, thus allowing efficient lipid preservation. Both procedures resulted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Endosperm; HEMA; Lipids; Plant anatomy; Plant microtechnique.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000200207

Morphology of fruits, seeds, seedlings and saplings of three species of Macrolobium Schreb. (Leguminosae, Caesalpinioideae) in the Brazilian Amazon floodplain
Provedor de dados: 39
Autores: Feitoza,Gildo Vieira; Santos,João Ubiratan Moreira dos; Gurgel,Ely Simone Cajueiro; Oliveira,Denise Maria Trombert.
The evaluation of the post-germination phases of plants allows the recognition of transitional structures that support the relationships among taxa and their establishment. Together with fruit and seeds, seedlings and saplings provide useful characters for identifying many species. This study aimed to describe and characterize the morphology of the fruits, seeds, seedlings and saplings of Macrolobium acaciifolium, M. bifolium and M. pendulum, as well as to evaluate the ecological, taxonomic and phylogenetic value of the structures identified. In this work, the seeds were sown in plastic trays with sterilized sand and sawdust, without pre-germination treatment. The fruits of the three species are woody legumes, dehiscent fruit or indehiscent fruit. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detarieae; Embryo; Fabaceae; Plumule; Post-germination development.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000300016

Genetics of the concentration of copper in common bean seeds - doi: 10.4025/actasciagron.v35i3.16520
Provedor de dados: 1
Autores: Poersch, Nerison Luís; Universidade Federal de Santa Maria; Ribeiro, Nerinéia Dalfollo; Universidade Federal de Santa Maria; Rosa, Daniele Piano; Universidade Federal de Santa Maria; Maziero, Sandra Maria; Universidade Federal de Santa Maria; Jost, Evandro; Instituto Federal de Educação, Ciência e Tecnologia Farroupilha.
The common bean (Phaseolus vulgaris L.) is an important source of copper in human nutrition. The objective of this research was to investigate the possible maternal effects on copper concentrations in the seeds of the common bean, to estimate the heritability and the selection gains in early hybrid generations for the characteristic of copper concentration and to evaluate the potential for genetic improvement. The controlled crossings were performed among IAPAR 44 x IAPAR 31 and Diamante Negro x TPS Bonito cultivars. The reciprocal F1, and F2 generations, as well as the backcross populations, were produced by each hybrid combination. The range of variation in the copper concentration among the tested progenies was from 1.07 to 7.75 mg kg-1 dry matter, and...
Palavras-chave: Agronomia Phaseolus vulgaris; Embryo; Maternal effect; Heritability; Early generation Melhoramento Vegetal.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16520

Morphology and anatomy of the diaspores and seedling ofPaspalum (Poaceae, Poales)
Provedor de dados: 42
Autores: EICHEMBERG,MAYRA T.; SCATENA,VERA L.
ABSTRACTThe knowledge regarding of the diaspore and post-seminal development ofPaspalum L. is important for grassland biodiversity conservation, based on their representativeness and genetic improvement of forage. The morphology of the diaspore and the post-seminal development of Paspalum dilatatum Poir. (rhizomatous),P. mandiocanum Trin. var. subaequiglume Barreto (stoloniferous), P. pumilum Nees. (decumbent caespitose) andP. urvillei Steud. (erect caespitose) was described to distinguish species with different growth forms and to survey the characters useful for taxonomy. P. dilatatum differs by presenting oval diaspores larger than the others, with five prominent nerves and trichomes; P. urvillei presents diaspores with one central nerve that is more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caryopsis; Embryo; Forage; Germination; Grass.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401389

Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil
Provedor de dados: 42
Autores: Freitas,Vicente J.F.; Serova,Irina A.; Andreeva,Lyudmila E.; Dvoryanchikov,Guennadi A.; Lopes-Jr.,Edilson S.; Teixeira,Dárcio I.A.; Dias,Luciene P.B.; Avelar,Suely R.G.; Moura,Raylene R.; Melo,Luciana M.; Pereira,Alexsandra F.; Cajazeiras,João B.; Andrade,Maria L.L.; Almeida,Karlliely C.; Sousa,Francisco C.; Carvalho,Antonio C.C.; Serov,Oleg L.
In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100µg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenesis; Goat; HG-CSF; DNA microinjection; Embryo.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000400003

Embryonic external morphogenesis of Rhammatocerus conspersus (Bruner) (Orthoptera: Acrididae: Gomphocerinae) and determination of the diapausing embryonic stage
Provedor de dados: 44
Autores: Niva,Cintia C.; Becker,Miriam.
The grasshopper Rhammatocerus conspersus (Bruner) (Orthoptera: Acrididae: Gomphocerinae) is an occasional pest in pasturelands of Rio Grande do Sul State. It is a univoltine species with an embryonic diapause. Nymphal and adult stages occur during the warmer months (November-March). Eggs were dissected periodically for characterization of embryonic external morphogenesis in 1994 and 1995. Ten embryonic stages were illustrated. Two diapausing stages were verified in R. conspersus: one at 25% and another at 50% of total embryonic development.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Grasshopper; Embryo; Diapause; Morphology.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000400010

Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed
Provedor de dados: 45
Autores: Sprícigo,J.F.W.; Leme,L.O.; Guimarães,A.L.; Oliveira Neto,J.C.; Silva,P.C.P.; Moreira,N.H.; Pivato,I.; Silva,B.D.M.; Ramos,A.F.; Dode,M.A.N.
ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming, blastocysts were in vitro cultured to assess re-expansion and hatching at 24 and 48 hours. Finally, at 48 hours, hatched blastocysts were submitted to RT-qPCR searching for BCL2A1, BAK, BAX and CASP3 genes. For MALDI-TOF, the ion intensity was expressed in arbitrary units. Blastocyst development was compared by Qui-square (P&lt; 0.05). Among the most representative PL was the phosphatidylcholine [PC (32:0) + H]+; [PC (34:1) + H]+ and [PC (36:4) + H]+. Beyond the PL, MALDI revealed some triglycerides (TG), including PPL (50:2) + Na+, PPO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; Embryo; Cryopreservation; Lipids; Apoptosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300837

Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation
Provedor de dados: 45
Autores: Araújo,M.C.C.; Vale Filho,V.R.; Ferreira,A.M.; Sá,W.F.; Barreto Filho,J.B.; Camargo,L.S.A.; Serapião,R.V.; Silva,M.V.G.B.
The effect of cryopreservation in IFN-tau, from bovine embryos produced in vitro was evaluated. Two treated groups (G1= fresh bovine embryos, n=59 and G2= freezed embryos, n=84) were used to study the effect of cryopreservation on IFN-tau secretion. After reaching the blastocyst phase, the embryos were kept on individual culture for additional period of 7 days. On days 3 and 7 after the beginning of embryos cultivation, samples of the media culture were taken for IFN-tau secretion titration. Oocysts taken from follicles ranging from 3 to 5mm in diameter were obtained from ovaries of females at slaughterhouse. The embryos were frozen, after being dehydrated with ethylene glycol (1.8m), conditioned on 0.5ml palletes and frozen. Frozen embryos secreted lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Embryo; PIV; Interferon-tau.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600008

Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
Provedor de dados: 45
Autores: Carvalho,R.; Passos,L.M.F.; Oliveira,A.M.; Henry,M.; Martins,A.S.
The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Semen; Embryo; EHV-1; EHV-4; PCR.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002

Bovine conceptus of Bos indicus produced by somatic cell nuclear transfer and parthenogenesis present morphological variations since the blastocyst stage
Provedor de dados: 45
Autores: Oliveira,F.D.; Sangalli,J.R.; Meirelles,F.V.; Perecin,F.; Silva Filho,P.P.C.; Watanabe,Y.F.; Miglino,M.A.; Assis Neto,A.C.
In cattle, embryo development is characterized by the appearance of two distinct cell layers, the trophectoderm and the inner cell mass. The latter will undergo differentiation to form the embryonic disc consisting of the epiblast and hypoblast. The aim of this study was to ultrastructurally characterize the bovine embryo from different in vitro production techniques, with emphasis on trophectoderm and inner cell mass cells. Bovine embryos on day 7 (conception = D1) of pregnancy, derived via in vitro production techniques, were fixed for light and transmission electron microscopy processing. Results suggested that embryos produced by nuclear transfer of somatic cells and parthenogenesis showed significant changes in macroscopic and microscopic structure....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo; Blastocyst; In vitro production; Ultrastructure.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601483

Influence of climatic conditions on in vitro production of bovine embryos
Provedor de dados: 45
Autores: Pessoa,G.A; Navarro,R.B; Rauber,L.P; Brass,K.E; Silva,C.A.M; Rubin,M.I.B.
Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P&lt;0.05) than in the following years. Average cleavage rates were always lower in winter (P&lt;0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cow; Oocytes; Embryo; Climate; In vitro prodution.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013

Reactive oxygen species in bovine embryo in vitro production
Provedor de dados: 47
Autores: Dalvit,G.C.; Cetica,P.D.; Pintos,L.N.; Beconi,M.T.
Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO2: 95% humidified air. In vitro fertilization was carried out in IVF-mSOF with frozenthawed semen in the same culture conditions and embryo in vitro culture in IVC-mSOF at 90% N2: 5% CO2: 5% O2. ROS was determined in denuded...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Reactive oxygen species; Oxidative stress; Oocyte; Embryo; Bovine.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200009

Advantages and Applications of Cryopreservation in Fisheries Science
Provedor de dados: 52
Autores: Tsai,S.; Lin,C.
Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014

Effect of Fibroblast Growth Factor 2 (FGF2) and Insulin Transferrin Selenium (ITS) on In Vitro Maturation, Fertilization and Embryo Development in Sheep
Provedor de dados: 52
Autores: Mondal,Sukanta; Mor,Avantika; Reddy,Ippala Janardana; Nandi,Sumanta; Parameswaragupta,PS.
The present study evaluated the effect of fibroblast growth factor 2 (FGF2) and insulin-transferrin-selenium (ITS) to the in vitro maturation and embryo culture media on ovine oocyte maturation, cleavage and embryo development. Oocytes having more than five layers of unexpanded cumulus cells and granular homogenous ooplasm were cultured into 50 μL droplets of eight different culture systems: (i) TCM-199 (Tissue Culture Medium-199); (ii) TCM-199+10 ng/mL FGF2; (iii) TCM-199+20 ng/mL FGF2; (iv) TCM-199+30 ng/mL FGF2; (v) TCM-199+10 ng/mL ITS; (vi) TCM-199+20 ng/mL ITS; (vii) TCM-199+30 ng/mL ITS and (viii) TCM-199+20 ng/mL ITS+20 ng/mL FGF2 in a CO2 incubator at 38.50C for 24 h. All the oocyte culture media were supplemented with 10% FBS, FSH (10 μg/mL) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast growth factor 2; Insulin transferrin selenium; Oocyte; Embryo; Sheep.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400521

Storage sites in seeds of Caesalpinia echinata and C. ferrea (Leguminosae) with considerations on nutrients flow
Provedor de dados: 52
Autores: Teixeira,Simone de Pádua; Machado,Sílvia Rodrigues.
The seeds of Caesalpinia echinata and C. ferrea behaved as typical endospermic seeds, despite their different morphological classification (exendospermic seeds were described for C. echinata and endospermic seeds for C. ferrea). Then, the aim of this work was to compare, under ultrastructural and histochemical terms, the nature of the storage substances and their accumulation sites, as well as the nutrient flow in seeds of these species. Cotyledons in C. echinata accumulate carbohydrates, lipids and proteins, which are mobilized from the outer to the inner parts as revealed by the position of plasmodesmata. Endosperm in C. ferrea accumulates carbohydrates and in C. echinata accumulates substances during the initial embryogenic phases. Such tissue develops...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotyledon; Embryo; Endosperm; Histochemistry; Reserve substances; Ultrastructure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100016

Egg Development of Paralichthys orbignyanus (Valenciennes, 1839)
Provedor de dados: 52
Autores: Cerqueira,Vinicius Ronzani.
Eggs of the Southern Brazilian flounder Paralichthys orbignyanus were obtained through spawning induction and reared in laboratory. The eggs released were free-floating or pelagic. Other important characteristics were: spherical shape, smooth chorion, narrow perivitelline space, and a single oil globule. Egg and oil globule diameter were about 0.792 ± 0.008 mm and 0.114 ± 0.003 mm, respectively. The blastodisc was observed approximately 1 h after fertilization and followed by blastodisc cleavage. Blastula stage started after about 5 h and gastrula stage after 9 h. Approximately 20 h after fertilization, blastopore closure was observed. Neurula or early embryo occurred after 24 h. Cardiac beats and movements of the free embryonic tail were recorded after 40...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flounder; Paralichthys orbignyanus; Egg; Embryo; Development.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300016

Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly
Provedor de dados: 62
Autores: VISINTIN,José Antônio; GARCIA,José Fernando; PANTANO,Thais; D’ÁVILA ASSUMPÇÃO,Mayra Elena Ortiz.
Compacted mouse morulae were frozen at 0.3ºC/min. or 0.5ºC/min. from -6ºC to -24ºC or -32ºC in 10% of glycerol plus different sucrose concentrations with or without 0.1% of honeybee royal jelly. Embryos were thawed in water bath at 22ºC for 20 seconds and cryoprotectant dilution was done in three steps. Embryos were cultured in Whitten’s medium for 24, 48 and 72 hours at 37ºC, 5% of CO2 and 100% of humidity. The in vitro development ranged from 56.6% to 100% after 72 hours. Expanded blastocysts were transferred to pseudopregnant recipients on the third day of the estrous cycle. Viable fetuses rates for embryos frozen to -24 or -32ºC at 0.3ºC/minute in 10% glycerol + 10% sucrose, 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly, 10% glycerol + 0.1%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Embryo; Cryopreservation; Sucrose; Royal jelly.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000400009

Cryopreservation of mouse morulae through different methods: slow-freezing, vitrification and quick-freezing
Provedor de dados: 62
Autores: MELLO,Marco Roberto Bourg de; QUEIROZ,Vinícius Seixas; LIMA,Alessandra Sobreira de; TAVARES,Liliam Mara Trevisam; ASSUMPÇÃO,Mayra Elena Ortiz D'Avila; WHEELER,Mathew B.; VISINTIN,José Antonio.
The in vitro and in vivo development of mouse morulae after cryopreservation through different methods was examined. The slow-freezing involved an equilibration in 1.5M ethylene glycol (EG) and cooled at 0.5; 0.7; 1.0 or 1.2ºC/minute. The vitrification involved a 3 minutes equilibration in 20% EG and 60 seconds in solution containing 40% EG, 18% ficoll and 10.26% sucrose. The quick-freezing involved an equilibration in 3M EG + 0.3M sucrose for 5 minutes and 2 minutes in nitrogen vapor. In all three methods the straws were thawed in air for 10 seconds and in water at 25ºC for 20 seconds and the embryos cultured in vitro for 72 hours to estimate blastocyst rate. To assess viability in vivo, frozen morulae as well as fresh embryos were transferred into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Embryo; Mouse.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000400003

Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes
Provedor de dados: 56
Autores: Aires,M.B.; Santos,J.R.A.; Souza,K.S.; Farias,P.S.; Santos,A.C.V.; Fioretto,E.T.; Maria,D.A.
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral yolk sac; Diabetes; Embryo; Pregnancy; Cell markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800676

Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
Provedor de dados: 65
Autores: Oliveira,Vinícius Vasconcelos Gomes de; Ramos,Rafael Antonio Nascimento; Ramos,Carlos Alberto do Nascimento; Guerra,Neurisvan Ramos; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon  Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Dog; Vertical infection; Embryo.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553

Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
Provedor de dados: 65
Autores: Oliveira,Maria Emilia Franco; Cordeiro,Mabel Freitas; Ferreira,Roberta Machado; Souza,Samuel Figueirêdo; Pieroni,Juliana Souza Pinto; Rodrigues,Luiz Fernando de Souza; Fonseca,Jeferson Ferreira da; Vicente,Wilter Ricardo Russiano.
The objective was to evaluate if supplemental LH given at the end of FSH treatment would synchronize the time of ovulation and increase the ovulation rate and embryo yield in Santa Ines ewes. Twenty superovulatory (SOV) programs were accomplished in cross-over design (60d interval). On D0, a CIDR device was inserted, and the device was replaced with a new one 7 days later, when 37.5µg of d-cloprostenol was administered. On D12, we started the SOV treatment, administering 256mg of pFSH 8 times, 12h apart. On D14, the CIDR was removed, and 200IU of eCG and 37.5µg of d-cloprostenol were administered. On D15, the ewes were allocated into one of two groups, a Control group (n=10) that received no supplemental LH and a LH group (n=10) treated with 7.5mg of LH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superovulation; LH; Ovulation; Embryo; Sheep.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021