Consulta: subjectFacets:"Enzyme"
Registros recuperados: 76
Data/hora: 05/06/2024 12:06:09

Modelos de curvas de deshidratado de plátano roatan en verde (Musa acuminata).
Provedor de dados: 32
Autores: Pérez Prado, José Adán.
El plátano es el frutal en segundo lugar en importancia económica en México, el 95% se destina al consumo nacional y el 5% restante a la exportación. No obstante, las pérdidas por manejo postcosecha justifican buscar métodos para conservarlo. El plátano presenta cambios en volumen debido al intercambio de calor y masa, por lo que decrece su superficie bajo la contracción de los tejidos por pérdida parcial de agua. En consecuencia, todos los parámetros que dependen de cambios de dimensión interna y externa, requieren del uso de modelos de simulación mediante curvas de secado que nos permitan describir dicho comportamiento en los alimentos. Por lo anterior, el presente trabajo presenta una serie de modelos de regresión (exponencial, lineal, logarítmico y...
Palavras-chave: Acido cítrico; Enzimas; Obscurecimiento; Harina; Madurez; Citric acid; Enzyme; Darkening; Flour; Ripening; Maestría Tecnológica; Agroindustria.
Ano: 2010
URL: http://hdl.handle.net/10521/331

Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
Provedor de dados: 69
Autores: Brandelli,Adriano; Riffel,Alessandro.
The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic...
Tipo: Journal article
Palavras-chave: Bacteria; Enzyme; Keratin; Protease.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007

Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene
Provedor de dados: 74
Autores: Facincani,Agda Paula; Ferro,Jesus Aparecido; Pizauro Jr.,João Martins; Pereira Jr.,Haroldo Alves; Lemos,Eliana Gertrudes de Macedo; Prado,Alessandro Luis do; Ferro,Maria Inês T.
The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, followed by cloning and expression studies of the enolase gene and determination of its activity. These studies showed that X. fastidiosa does not use the glycolytic pathway to metabolize carbohydrates, which explains the increased duplication time of this phytopatogen. Recombinant enolase was expressed as inclusion bodies and solubilized with urea (most efficient extractor), Triton X-100, and TCA....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enolase; Entner-Doudoroff; Enzyme; Glycolysis; Xylella fastidiosa.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200015

Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides
Provedor de dados: 5
Autores: Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M.
To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is...
Tipo: Text
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf

Adaptation du métabolisme respiratoire de l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Le Moullac, Gilles.
Summer mortality of the oyster result from the interaction of many factors. A respiratory assumption proposes a link between the supposed causes of the energy state, the cultivation methods and the medium. This work is focused towards the search for specific metabolic markers of environmental stresses. The cDNA of genes coding for PK and PEPCK were cloned and sequenced in order to detect their regulation level. The sediment nearness affects the state of energy reserves, the metabolic pathways of energy production implying a regulation of the PK. Face to the dissolved oxygen decline, oysters were oxyregulator but with a low regulation ability. In hypoxia, glycolysis was slowed down. PK activity was inhibited resulting from an increase in alanine sensitivity...
Tipo: Text
Palavras-chave: Régulation; Enzyme; Expression génique; Anoxie; Bivalve.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-4315.pdf

Comparison of growth, digestive system maturation and skeletal development in sea bass larvae reared in an intensive or a mesocosm system
Provedor de dados: 5
Autores: Zouiten, Dora; Ben Khemis, Ines; Masmoudi, Ahmed Slaheddin; Huelvan, Christine; Cahu, Chantal.
The quality of development in intensive or mesocosm hatchery-reared Dicentrarchus labrax larvae was investigated using physiological indicators assessing ontogeny. Larvae were reared in intensive (120 L tanks) and in mesocosm systems (20 m(3) enclosures) with the same feeding sequence, excluding the wild zooplankton naturally available in mesocosms. Faster growth was recorded since early development [16 day after hatching (DAH)] in the mesocosm. Maturation of the digestive system also occurred earlier as indicated by the higher amylase secretion ratios, the intestinal maturation index (alkaline phosphatase/leucine-alanine peptidase and aminopeptidase-N/leucine-alanine peptidase ratios) and the more developed intestinal epithelium at 23 DAH. Nevertheless,...
Tipo: Text
Palavras-chave: Fish larvae; Hatchery; Mesocosm; Ontogenesis; Enzyme; Skeletogenesis.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00051/16225/14064.pdf

Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment
Provedor de dados: 5
Autores: Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened...
Tipo: Text
Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf

Impacts of three different microdiets on Florida Pompano, Trachinotus carolinus, weaning success, growth, fatty acid incorporation and enzyme activity.
Provedor de dados: 5
Autores: Hauville, Marion; Zambonino, Jose Luiz; Bell, G; Migaud, Herve; Main, Kevan L.
In this study, three microdiets were tested on weaning of Florida pompano larvae: Otohime, Gemma and a reference diet LR803. The experimental system was stocked with 11-day-old larvae, which were co-fed micro-diets and live food from 11 dph to 17 dph then micro-diets only until 28 dph. Survival from 11 dph to 28 dph was similar for all treatments, with an average of 33%. At the end of the trial, the Gemma larvae were significantly longer and heavier than larvae fed the other diets. Significant differences were observed in fatty acid composition of the diets and larvae between treatments. The Gemma larvae incorporated the lowest amount of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (ARA). However, they had the highest...
Tipo: Text
Palavras-chave: Enzyme; Fatty acids; Fish larvae; Microdiets; Trachinotus carolinus; Weaning.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00169/28067/26282.pdf

Optimization of common cuttlefish (Sepia officinalis) protein hydrolysate using Pepsin by Response Surface Methodology
Provedor de dados: 5
Autores: Kechaou, Emna; Berge, Jean-pascal; Jaouen, Pascal; Ben Amar, Raja.
Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of prediction model. An enzyme activity to substrate ratio (X1), 0.04 AU/g protein; time (X2), 85 minutes; and temperature (X3), 45 °C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using Pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order....
Tipo: Text
Palavras-chave: Cuttlefish protein hydrolysate (CPH); Amino acid; Enzyme; Sepia officinalis.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00182/29285/27715.pdf

De l'utilisation de protéases industrielles pour la production de sauces de poisson
Provedor de dados: 5
Autores: Le, Minh Chau.
Fish sauces are traditionally used in most Asian countries. Whatever the process, the principle is the same i.e. a fish autolysis in saline media followed by a fermentation step. Not less than 18 months are needed to obtain a brown liquid rich in salt and amino acids. This PhD work aims to determine if an addition of proteases can speed up the process while keeping the nutritional and sensorial properties. The first chapter presents the characteristics of fish sauces and the key parameters of the processes used. In the second chapter, several industrial proteases are compared each other for their capacity to liquefy the fishes in hyper saline conditions. Kinetics of proteolysis and biochemical characteristics of the resulting hydrolysates are established...
Tipo: Text
Palavras-chave: Sauce de poisson; Hydrolyse; Enzyme; Protéase; Sardine; Anchois; Fish sauce; Hydrolysis; Enzyme; Protease; Sardine; Anchovy.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00259/36997/35488.pdf

Attendrissement enzymatique de chair de mollusques
Provedor de dados: 5
Autores: Kouadio Kouakou, François.
Two types of marine molluscs were studied. The highly individual characteristics of the flesh of these two shellfish, in this case its firmness, led us to analyse the behaviour of the foot muscle, the firmest part, as a function of heat and to the determination of the different temperatures at which the denaturation of the main constituent proteins occurs. This firmness makes the muscles difficult to chew to such an extent that they have acted as a break to consumption.  These shell-fish have very similar proportions of myofibrillar and connective proteins, both of- which are responsible for the texture of meat. This similarity of composition determined the choice of an enzymatic preparation (protease V100) capable of acting simultaneously on both these...
Tipo: Text
Palavras-chave: Glycymeris glycymeris; Buccinum undatum; Texture; Attendrissement; Enzyme; Glycymeris glycymeris. Buccinum undatum; Texture; Tenderizing; Enzyme.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/00030/14134/11381.pdf

Changes in the Activity of Enzymes Primarily Not Synthesized in the Liver Following the Infection Rabbits with Intestinal Coccidia
Provedor de dados: 19
Autores: Kulišić, Zoran; Tambur, Zoran; Maličević, Živorad; Radosavljević, Milan.
Palavras-chave: Rabbit; Enzyme; Intestinal coccidia.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/273

The Bread making qualities of bread dough supplemented with whole wheat flour and treated with enzymes
Provedor de dados: 19
Autores: Matsushita, Koki; Santiago, Dennis Marvin; Noda, Tatsuya; Tsuboi, Kazumasa; Kawakami, Sakura; Yamauchi, Hiroaki.
Whole wheat flour (WWF) contains various kinds of functional ingredients, such as dietary fiber, resistant starch, minerals, etc., and bread containing WWF is in great demand in many countries because of its health benefits. Although dough with WWF often results in lower quality bread, previous studies suggested that this could be improved by the addition of suitable enzymes. This study investigated the effects of WWF substitution and enzyme treatments using alpha-amylase (AM) and hemicellulase (HC) on bread making qualities. Results showed that the addition of WWF produced dough with low gas retention of dough (GRD) and specific loaf volume (SLV). However, AM and HC drastically improved both GRD and SLV of WWF-substituted dough and bread by degrading...
Palavras-chave: Whole wheat flour; Bread making quality; Alpha-amylase; Hemicellulase; Enzyme.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4486

Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation
Provedor de dados: 87
Autores: Osuna,M.D.; Goulart,I.C.G.R.; Vidal,R.A.; Kalsing,A.; Ruiz Santaella,J.P.; De Prado,R.
Eleusine indica (goosegrass) is a diploid grass weed which has developed resistance to ACCase inhibitors during the last ten years due to the intensive and frequent use of sethoxydim to control grass weeds in soybean crops in Brazil. Plant dose-response assays confirmed the resistant behaviour of one biotype obtaining high resistance factor values: 143 (fenoxaprop), 126 (haloxyfop), 84 (sethoxydim) to 58 (fluazifop). ACCase in vitro assays indicated a target site resistance as the main cause of reduced susceptibility to ACCase inhibitors. PCR-generated fragments of the ACCase CT domain of the resistant and sensitive reference biotype were sequenced and compared. A point mutation was detected within the triplet of aspartate at the amino acid position 2078...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goosegrass; Cross-resistance; Enzyme; PCR; Herbicide.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000300025

Nutritional Evaluation of Soybean Hulls with or without β-Mannanase Supplement on Performance, Intestinal Morphometric and Carcass Yield of Broilers Chickens
Provedor de dados: 89
Autores: Scapini,LB; Rorig,A; Ferrarini,A; Fülber,LM; Canavese,M; Silva,AM; Fernandes,JIM.
ABSTRACT The objective was to evaluate the performance, intestinal morphology and carcass yield of broilers fed corn-soybean meal (SBM) diet or corn-SBM-soybean hull (SH) with or without b-mannanase supplementation. Thousand four hundred and forty Cobb Slow-male- chicks were housed design following a factorial scheme 2 x 2 (corn-SBM diet and corn-SBM-SH diet vs with and without b-mannanase), composing 4 treatments and 9 replicates each treatment, with 40 birds each replicate. At 21 days, corn-SBM diet supplemented with b-mannanase resulted in better (p&lt;0.05) feed conversion. At 42 days, the weight gain (p&lt;0.05) and feed intake (p&lt;0.05) of the birds fed diets containing SH was 2.6% and 2.9% higher than that of birds fed corn-SBM diets,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean meal; Intestinal mucosa; Enzyme; Non-starch polysaccharides.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400633

The Role of ß-Mannanase (Hemicell) in Improving Poultry Productivity, Health and Environment
Provedor de dados: 89
Autores: Saeed,M; Ayaşan,T; Alagawany,M; El-Hack,MEA; Abdel-Latif,MA; Patra,AK.
ABSTRACT The poultry nutritionists constantly attempt to identify and alleviate the factors responsible for adverse effects on nutrient utilization and production performance in avian species, which are necessary for successful productivity of poultry. Enhanced feed utilization can reduce levels of some nutrients in the diet with concomitant mitigation in nutrient excretion into the environment, reduction of eutrophication and acidification potentials of excreta. Commercial enzymes have been used to improve feed efficiency and utilization in poultry. Among many anti-nutritional factors, the existence of ß-mannans in poultry feed ingredients including soybean and other leguminous seeds has been associated with negative effects on nutrient digestibility and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-nutrient factor; SS-mannanase; Enzyme; Feed utilization; Poultry.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300303

Hybrid Phytase and Carbohydrases in Corn and Soybean Meal-Based Diets for Broiler Chickens: Performance and Production Costs
Provedor de dados: 89
Autores: Bavaresco,C; Krabbe,E; Gopinger,E; Sandi,AJ; Martinez,FN; Wernik,B; Roll,VFB.
ABSTRACT This study aimed to evaluate the effect of using 500 FTU/kg of hybrid phytase (created from three bacteria - Natuphos E), with or without xylanase and glucanase (560 TXU/kg; 250 TGU/kg) - Natugrain TS, in corn and soybean meal-based diets, with nutritional reductions in metabolizable energy (ME), Ca, and available phosphorus (AP), on performance parameters and production costs in broiler chickens. The 1875chickens were housed in boxes and distributed among 5 treatments with 15 replicates of 25 chickens each. The experiment included a positive control (the diet of which met the nutritional requirements of the birds), two negative controls (with ME reductions of 70 and 100 kcal/kg, and fixed reductions in Ca [0.16%] and AP [0.15%]), and two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Cost; Energy; Enzyme; Phosphorus.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100319

Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
Provedor de dados: 89
Autores: Oliveira,GH; Berchieri Jr,A; Montassier,HJ.
This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; ELISA; Enzyme; Salmonella sp; Serology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008

Efficacy and Metabolizable Energy Equivalence of an α-Amylase-β-Glucanase Complex for Broilers
Provedor de dados: 89
Autores: Vieira,SL; Stefanello,C; Rios,HV; Serafini,N; Hermes,RG; Sorbara,JOB.
A trial was conducted to evaluate the effects of adding an exogenous α-amylase-β-glucanase complex produced from Bacillus amyloliquefaciens on the growth performance, carcass yield, and relative AME bioequivalence in broilers fed corn-soy diets from 1 to 40 d of age. One thousand seven hundred and fifty one-day-old Cobb x Cobb 500 slow-feathering male broilers were randomly allotted to seven treatments with 10 replicates of 25 birds each as follows: control diet (C); C diet with reduction of 60 (C-60), 90 (C-90), or 120 (C-120) kcal AME/kg; C diet with reduction of 120 kcal AME/kg and supplemented with 200 (C-120-200), 300 (C-120-300), or 400 (C-120-400) mg of the enzyme complex/kg. Each g of the enzyme complex corresponded to 200 kilo-Novo α-amylase and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Β-glucanase; Broiler; Carbohydrases; Enzyme.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000200227

Effects of Bile Acids and Lipase Supplementation in Low-Energy Diets on Growth Performance, Fat Digestibility and Meat Quality in Broiler Chickens
Provedor de dados: 89
Autores: Arshad,MA; Bhatti,SA; Hassan,I; Rahman,MA; Rehman,MS.
ABSTRACT The aim of this study was to investigate the effect of bile acids and lipase supplementation in low energy (LE) diets on growth, fat digestibility, serum lipid profile and meat quality of broilers. Seven hundred one-day-old broiler chicks were divided into 5 dietary treatments with five replicates of 28 birds each. The five treatments were: i) high energy diet (HE; metabolizable energy (ME) = 3,000 and 3,170 kcal/kg for starter and finisher diet), ii) low energy diet (LE; ME = 2,900 and 3,070 kcal/kg for starter and finisher diet), iii) LE diet supplemented with 300 g/ton bile acids (LEB), iv) LE diet supplemented with 180 g/ton lipase (LEL), v) LE diet supplemented both with bile acids (300 g/ton) and lipase (180 g/ton). The experiment lasted 35...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digestibility; Enzyme; Energy density; Fat; Meat quality.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200315

Effects of cereal type, enzyme and sodium butyrate addition on growth performance, carcass traits and intestinal morphology of broilers
Provedor de dados: 89
Autores: Shahir,MH; Moradi,S; Afsarian,O; Esmaeilipour,O.
A 2 × 2 × 2 factorial arrangement was conducted to evaluate the effects of cereal type (corn vs. wheat), enzyme supplementation (0 or 0.4 g/kg diet), and sodium butyrate addition (0 or 2 g/kg diet) on growth performance, carcass traits and intestinal morphology of male broilers from 10 to 42 d. A total of 600 male Arbor Acres chickens were assigned to eight dietary treatments in a completely randomized design, providing five replicate pens per treatment. Diets were formulated to meet or exceed NRC nutrient recommendations. When compared with birds fed corn-based diets, chickens fed wheat-based diets had inferior weight gain (1978 vs. 1807 g, p &lt; 0.01) and feed per gain ratio (1.81 vs. 1.93; p &lt; 0.01). Both weight gain (1870 vs. 1915 g; p &lt; 0.01)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Sodium butyrate; Performance; Intestinal morphology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000300003

Effect of ph and temperature on the activity of phytase products used in broiler nutrition
Provedor de dados: 89
Autores: Naves,L de P; Corrêa,AD; Bertechini,AG; Gomide,EM; Santos,CD dos.
The activity of three commercial microbial phytase (Aspergillus oryzae, A. niger, and Saccharomyces cerevisae) products used in broiler nutrition was determined at different pH (2.0 to 9.0) and temperature (20 to 90°C) values. Enzymatic activity was determined according to the reaction of the phytase with its substrate (sodium phytate), in four replicates, and was expressed in units of phytase activity (FTU). A. oryzae phytase exhibited optimal activity at pH 4.0 and 40°C, but its absolute activity was the lowest of the three phytases evaluated. A. niger phytase exhibited maximal activity close to pH 5.0 and 45ºC, whereas S. cerevisae phytase presented its highest activity at pH close to 4.5 and temperatures ranging between 50 and 60°C. It was concluded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic activity; Enzyme; Phytate; Phytic acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000300004

Enzyme Complex Added to Broiler Diets: Effects on Performance, Metabolizable Energy Content, and Nitrogen and Phosphorus Balance
Provedor de dados: 89
Autores: Pessôa,GBS; Ribeiro Junior,V; Albino,LFT; Araújo,WAG; Silva,DL; Hannas,MI; Rostagno,HS.
ABSTRACT Two trials were performed to evaluate the effects of an enzyme complex (EC) on broiler performance, metabolizable energy, and phosphorus and nitrogen balance. In the first trial, 960 one-day-old male Cobb broilers were randomly distributed in a 3 x 2 factorial arrangement (three nutritional levels and the inclusion or not of EC), totaling six treatments with eight replicates of 20 birds per pen, and reared until 42 days of age. The control diet was formulated to meet the broilers' nutritional requirements. The other diets contained reduced available phosphorus (P), metabolizable energy, and amino acid levels, considering 100% and 150% of the EC nutritional matrix. The inclusion of the EC (200 g/ton) resulted in better broiler performance,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additives; Broiler; Enzyme.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300467

Effects of Plant Essential Oils on Vitamin C, Malondialdehyde and Some Biochemical Parameters of Rats*
Provedor de dados: 89
Autores: Göze,I; Erşan,S; Aydin,H; Ercan,N; Dönmez,E.
ABSTRACT The aim of this study was to determine the LD30 values of the essential oils of Origanum minutiflorum He Schwarz-PH Davis (OM) and Juniperus excelca by Bieb.subsp. Excel (JE) in vivo investigation on the effects of malondialdehyde (MDA), vitamin C and some biochemical parameters. In this, study the essential oils of OM and JE plants were used. LD50 values of the essential oils were determined by using rats. OM (n=10), JE (n=10), carvacrol (n=10) which dissolve in olive oil were used as experimental group and as control group saline (SF) (n=8) and solvent Olive oil (n=10) were used and applied intraperitoneal on rats for 12 days in LD30 dosages. In the end of 12 days, Vit-C, malondialdehyde (MDA) and routine biochemical analyses were studied on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Origanumminutiflorum O Schwarz-PH Davis; Juniperusexcelca Bieb.subsp.excelca; Essential oil; Vitamin C; Malondialdehyde; Enzyme.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200306

Evaluation of nutrient excretion and retention in broilers submitted to different nutritional strategies
Provedor de dados: 89
Autores: Graña,AL; Tavernari,FC; Lelis,GR; Albino,LFT; Rostagno,HS; Gomes,PC.
An experiment was carried out to evaluate the effects of different nutritional strategies on nitrogen (N), phosphorus (P) and calcium (Ca) balance and on copper (Cu), manganese (Mn) and zinc (Zn) excretion in broilers during the periods of 1 to 21 days and 1 to 46 days of age. Four hundred male Cobb-500 broilers were used. A randomized block experimental design was applied, including five treatments with eight replicates of 10 birds each. A five-phase feeding program was adopted (1-8, 9-21, 22-33, 34-40 and 41-46 days of age). Treatments consisted of a control diet (C) with typical protein level and low amino acid supplementation; a reduced-protein diet supplemented with synthetic amino acids formulated on ideal protein concept (IP); C with phytase (C+PHY)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Phosphorus; Organic trace minerals; Ideal protein.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000200013

Heat-resistant bacterial phytase in broiler pelleted diets
Provedor de dados: 89
Autores: Carlos,TC de F; Barbosa,LCGS; Shiroma,NN; Dari,RL; Baolin,G; Yongcheng,W; Araújo,CS da S; Araújo,LF.
The objective of the study was to evaluate the effects of a heat-resistant bacterial phytase added to pelleted diets on mineral digestibility, live performance, carcass traits, and bone quality of broilers. Three treatments were evaluated: Positive control; negative control, with 0.10 points reduction in calcium level and 0.15 points reduction in available phosphorus level; and negative control + phytase at 500 FTU/kg. Mineral digestibility and bone quality results demonstrated that the evaluated phytase resisted pelleting as it increased the utilization of the minerals present in the diet.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Performance; Digestibility; Enzyme; Phytate; Phosphorus; Broiler nutrition.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000100045

Energy and protein levels in diets containing phytase for broilers from 22 to 42 days of age: performance and nutrient excretion
Provedor de dados: 96
Autores: Nagata,Adriano Kaneo; Rodrigues,Paulo Borges; Alvarenga,Renata Ribeiro; Zangeronimo,Márcio Gilberto; Rodrigues,Kênia Ferreira; Lima,Gustavo Freire Resende.
This study was conducted in order to evaluate the influence of different levels of metabolizable energy and crude protein in diets formulated according to the ideal protein concept with phytase supplementation on performance and nutrient excretion of broilers from 22 to 42 days age. It was used 1,500 Coob lineage broilers at 22 days of age and with initial weight of 833 ± 7g, distributed in completely randomized design in a 3 × 3 + 1 factorial scheme composed of three levels of correct apparent metabolizable energy (2,950; 3,100 and 3,250 kcal/kg), three levels of crude protein (14, 16 and 18%) and a control treatment, totaling ten treatments with six repetitions of 25 birds each. All diets, with the exception of the control, were supplemented with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Addictive; Available phosphorus; Enzyme; Ideal protein; Metabolizable energy; Pollutants.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000800014

How can nutritional strategies and feed technologies in pig production affect the logistical costs of manure distribution?
Provedor de dados: 96
Autores: Afonso,Esther Ramalho; Nacimento,Rafael Araujo; Palhares,Julio Cesar Pascale; Gameiro,Augusto Hauber.
ABSTRACT The objective with this study was to evaluate the impact of different swine nutritional strategies and technologies, as well as the land spread system, on logistical costs of pig manure distribution. For this, pigs were fed a conventional diet (C0) or a diet supplemented with 0.01% phytase (C1); organic minerals (40% substitution; C2); synthetic amino acids at reduced dietary crude protein levels (C3); or a combination of the three strategies (C4). All pig manure was weighed, then its chemical composition was determined. The mineral values and volume of manure produced were extrapolated to corresponding pig farm sizes in Sao Paulo state (300, 650, and 1000 sows) from real pig manure levels to produce simulations. To determine the logistical cost...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Organic mineral; Swine; Waste.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982020000100100

Fiber source and xylanase on performance, egg quality, and gastrointestinal tract of laying hens
Provedor de dados: 96
Autores: Sousa,Lorena Salim de; Carvalho,Thiago Soares Martins; Nogueira,Flávia Aparecida; Saldanha,Mariana Masseo; Vaz,Diego Pereira; Bertechini,Antônio Gilberto; Baião,Nelson Carneiro; Lara,Leonardo José Camargos.
ABSTRACT The objective of this study was to ascertain the influence of different dietary fiber sources and the usage of xylanase on diet of commercial layers and their influence on productive performance, egg quality, and digestive organ biometry. A total of 864 Lohmann® White hens was fed diets with three different fiber sources (wheat bran, soybean hull, or coffee husk) with or without xylanase inclusion (concentration of 160,000 BXU/g) in a 3×2 factorial arrangement, with six replicates of 24 birds each, from 25 to 44 weeks of age. There were no interactions between dietary fiber and xylanase inclusion. The enzyme supplementation did not influence any parameters evaluated. There were dietary fiber effects on body weight gain, viability, egg weight,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative fiber; Enzyme; Laying hen; Non-starch polysaccharides; Viscosity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100504

Levels of available phosphorus and calcium for broilers from 8 to 35 days of age fed rations containing phytase
Provedor de dados: 96
Autores: Cardoso Júnior,Adimar; Rodrigues,Paulo Borges; Bertechini,Antônio Gilberto; Freitas,Rilke Tadeu Fonseca de; Lima,Renato Ribeiro de; Lima,Gustavo Freire Resende.
Two experiments using Cobb broiler chicks from 8 to 35 days of age were carried out to evaluate the effect of reducing levels of calcium and available phosphorus in diets supplemented with 500 ftu phytase/kg on the performance of the birds, the content of ashes in the tibia, the metabolizable energy of the diet, and the metabolizability of the dry matter and crude protein. It was used 1,404 broiler chicks in the experiment 1 to evaluate feed intake, weight gain, food conversion and percentage of the ashes in the tibia. In the experiment 2, simultaneously carried out with experiment 1, a total of 390 birds were transferred to a metabolism room to determine the metabolizable energy and metabolizability of the dry matter and crude protein. It was used in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Minerals; Nutritional requirement.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000600011

Supplementation of fungal and/or bacterial phytase in broiler diets formulated with reduced phosphorus level and different calcium contents
Provedor de dados: 96
Autores: Nardelli,Nicole Batelli de Souza; Naves,Luciana de Paula; Oliveira,David Henrique de; Garcia Junior,Antonio Amandio Pinto; Lima,Eduardo Machado Costa; Oliveira,Evelyn Cristina de; Rodrigues,Paulo Borges.
ABSTRACT The objective of the current study was to evaluate the effects of three calcium:available phosphorus (Ca:aP) ratios and different supplementation with phytases from different origins on performance and ash, Ca, and P contents in the tibia of broilers. A total of 900 male broilers (22 to 35 days old) were fed ten dietary treatments in a completely randomized design. A 3 × 3 + 1 factorial scheme was used, corresponding to three Ca:aP ratios (4.5:1.0, 6.0:1.0, and 7.5:1.0) and three different supplementations with phytases from different origins (isolated or combined supplementation with bacterial and fungal phytase), plus a control diet. Regardless of the dietary Ca:aP ratio, the isolated use of bacterial phytase provided better feed conversion than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Nutrition; Phytate; Phytic acid.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100535

Metabolic and histologic responses of pacu (Piaractus mesopotamicus) fed diets supplemented with increasing concentrations of ractopamine
Provedor de dados: 96
Autores: Drumond,Mariana Martins; Naves,Luciana de Paula; Ribeiro,Paula Adriane Perez; Oliveira,Marinez Moraes de; Okamura,Daniel; Resende,Anaise Emanuele; Cantarelli,Vinicius de Souza; Rosa,Priscila Vieira.
ABSTRACT An experiment was conducted during 60 days with forty pacu males fed diets supplemented with increasing concentrations of ractopamine (0.00, 11.25, 22.50, 33.75, and 45.00 mg kg−1). Eight fish were evaluated for each experimental diet. Performance and survival rate of the fish were measured. At the end of the experiment, blood was collected to determine the levels of cortisol, triacylglycerol, and protein. Moreover, the liver was collected to determine the activities of glucose-6-phosphate dehydrogenase and malic enzymes. The fillets were collected to determine chemical composition, and histologic cuts were analyzed to verify muscle growth and deposition of adipose tissue between muscle fibers. Increasing concentrations of dietary ractopamine did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta-adrenergic agonist; Enzyme; Metabolism; Stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100100

Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease
Provedor de dados: 96
Autores: Cardinal,Kátia Maria; Moraes,Mariana Lemos de; Andretta,Ines; Schirmann,Graciele Dalise; Belote,Bruna Luiza; Barrios,Miguel Alejandro; Santin,Elizabeth; Ribeiro,Andréa Machado Leal.
ABSTRACT We evaluated the effects of a protease supplementation on the growth performance and intestinal health of broilers. Cobb chicks (392; 1-42 d) were divided into four treatments (seven replicates of 14 birds each). There were two feed formulations: a standard diet (SD) and a low crude protein and digestible amino acids diet (Low CP&amp;AA). The two diets were either supplemented (+P) or not (−P) with a protease (Jefo Protease; 1.25 g kg−1). Performance was evaluated by feeding phases (1-7, 8-21, 21-35, and 35-42 d). On day 28, ileum samples were analyzed by a morphometric index for histological alterations (I See Inside Scoring System – ISI). Broilers fed the Low CP&amp;AA had a poor feed conversion ratio (FCR); however, the addition of the protease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aminoacid; Animal nutrition; Digestive physiology; Enzyme; Feed additive.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100524

Dietary phytase levels on performance and egg quality of Japanese quails
Provedor de dados: 96
Autores: Lima,Heder José D'Avila; Barreto,Sergio Luiz de Toledo; Donzele,Juarez Lopes; Valeriano,Matheus Henrique; Vieira,Patrícia Aparecida Fontes; Costa,Carlos Henrique Rocha.
The experiment was conducted to evaluate the addition of phytase on productive performance and egg quality of Japanese quails fed diets containing different levels of phytase. It was used 320 Japanese quails at 167 days of age, weighing 182.3 ± 3.8 g distributed in a completely randomized design with four experimental diets and eight repetitions of ten birds per experimental unit. Diets were formulated from a basal diet (control) and they were corn and soybean meal based, without supplementation, added with 200, 400 or 600 phytase activity unit (ftu). Supplementation of phytase in the diets improved productive performance and quality of eggs. Efficiency of phosphorus use for egg mass, from 0.13% of availability of this mineral in the ration, improved with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coturnix coturnix japonica; Enzyme; Nutritional profile; Phosphorus; Phytate.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000100018

Nutrient reduction in rations with phytase for growing pigs
Provedor de dados: 96
Autores: Rodrigues,Valéria Vânia; Cantarelli,Vinícius de Souza; Amaral,Nikolas de Oliveira; Zangeronimo,Márcio Gilberto; Brito,Jeronimo Ávito Gonçalves de; Fialho,Elias Tadeu.
Two experiments were conducted to evaluate the performance, the energy and nutrient balance and economic viability of production of growing pigs fed diets with reduced levels and phytase. In experiment 1, 60 barrows and gilts (initial weight 25 ± 1.72 kg) were housed in a randomized block design with three diets, ten replicates and two animals per experimental unit (one male and one female). One of the diets was formulated with a reduced level with phytase and evaluated in comparison to a positive-control diet that was formulated according to the nutritional recommendations for animals of this breed, and a negative-control diet without phytase. The different diets did not affect feed intake, but the diet with phytase promoted better final weight, daily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diet formulation; Enzyme; Metabolism; Nutrition; Performance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000200019

Effects of silicon and drought stress on biochemical characteristics of leaves of upland rice cultivars
Provedor de dados: 124
Autores: Mauad,Munir; Crusciol,Carlos Alexandre Costa; Nascente,Adriano Stephan; Grassi Filho,Hélio; Lima,Giuseppina Pace Pereira.
ABSTRACT Silicon (Si) has beneficial effects on many crops, mainly under biotic and abiotic stress. Silicon can affect biochemical, physiological, and photosynthetic processes and, consequently, reduce drought stress. However, the effects of Si on rice (Oryza sativa L.) plants under drought stress are not well known. The objective of this study was to evaluate the effects of supplemental Si on proline content and peroxidase activity in upland rice plants in the presence and absence of drought stress. The experiment was conducted under greenhouse conditions and was arranged in completely randomized blocks in a 2 x 2 x 2 factorial scheme. Treatments comprised combinations of (1) presence or absence of Si supply (0 or 350 kg ha-1 of Si), (2) presence or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Proline; Peroxidase; Enzyme; Hydric deficiency.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000300532

Genetic analysis of greenbug populations of Schizaphis graminum (Hemiptera: Aphididae) from Argentina and Chile based on enzyme variability
Provedor de dados: 99
Autores: Saldúa,Luciana; Tacaliti,María S.; Tocho,Erica; Dixon,Anthony F. G.; Castro,Ana M.
Twenty-nine Schizaphis graminum (Rondani) populations and sixty clones collected from very contrasting regions of Argentina and Chile were investigated electrophoretically. A high degree of enzymatic polymorphism was found. The enzymatic structure was described for the esterase system, finding nine different loci. Latitudinal stratification was determined and populations were associated into three groups, according to the latitude they were collected from. The 90% of loci resulted polymorphic in the first group and 100% of loci were polymorphic in the rest. Observed heterozygosity was lower than expected. No group had fixed alleles according to Fsr values; group one had a slight heterozygous excess, while the other groups showed slight positive Fsr values,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schizaphis graminum; Enzyme; Genetic flow; Genetic-structure; Geographical association.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802011000100008

Nitrate reductase activity in leaves and stems of tanner grass (Brachiaria radicans Napper)
Provedor de dados: 63
Autores: Cazetta,Jairo Osvaldo; Villela,Luciana Cristine Vasques.
Tanner grass (Brachiaria radicans Napper) is a forage plant that is adapted to well-drained soils or wetlands, and responds well to nitrogen (N) fertilization. The assimilation of N involves the nitrate reductase (NR) enzyme, and its activity seems to be dependent on N supply. Molybdenum (Mo) is also important because it is a cofactor of NR. In this study, the variables of an in vivo assay were optimized for measuring nitrate reductase activity (NRA) in the leaves and stem tissues. This method was used to evaluate NO3- metabolism in plants fertilized with NaNO3, NH4Cl or urea, in association with or without application of H2MoO4, aiming to provide guidelines for N management of this species. The best conditions to determine NRA involved the incubation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachiaria arrecta; Nitrogen; Molybdenum; Enzyme.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000600012

Extraction, partial characterization and susceptibility to Hg2+ of acid phosphatase from the microalgae Pseudokirchneriella subcapitata
Provedor de dados: 63
Autores: Jonsson,Claudio Martín; Aoyama,Hiroshi.
Pseudokirchneriella subcapitata is a unicellular green algae widely distributed in freshwater and soils. Due to its cosmopolitan characteristic, its use is recommended by national and international protocols in ecotoxicity studies. The alteration of phosphatase activities by agriculture pollutants like heavy metals has been extensively used as a biomarker in risk assessment and biomonitoring. In this study, we compared the extraction of acid phosphatase from P. subcapitata by different procedures and we studied the stability, substrates specificity, kinetics and the effect of Hg2+ in the crude extract. The freezing and thawing technique associated with probe sonication was the most suitable method of extraction. The enzyme was stable when frozen at -20ºC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Selenastrum capricornutum; Algae; Enzyme; Metal; Toxicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500008

Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation
Provedor de dados: 63
Autores: D'Agostini,Érica Clarissa; Mantovani,Talita Rafaele D'Agostini; Valle,Juliana Silveira do; Paccola-Meirelles,Luzia Doretto; Colauto,Nelson Barros; Linde,Giani Andrea.
Basidiomycetes are laccase producers used for hydrolysis of lignocellulosic byproducts in fermentative processes and could be used on biofuel production or ruminant feeding. The objective of this study was to evaluate the effect of concentrations of non-protein nitrogen sources on laccase production and mycelial growth of Pleurotus ostreatus, Lentinula edodes and Agaricus blazei. The fungi were grown on soybean hulls to which urea (U), ammonium sulfate (AS) or mixture of AS:U (1:1) were added to achieve carbon/nitrogen (C/N) ratios of 5, 15, 20 or 30. The average longitudinal mycelial growth was measured and laccase activity was determined by the oxidation of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid. Higher C/N ratios increased mycelial growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Industrial byproduct; Lignocellulolytic; Enzyme; Lignolytic; Soybean hull.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300004

กิจกรรมเอนไซม์ซูเปอร์ออกไซด์ดิสมิวเทสในหัวแก่นตะวันสายพันธุ์ต่างๆ
Provedor de dados: 106
Autores: Anoma Dongsansuk; Sanan Jogloy.
The study of SOD activity in Jerusalem artichoke tubers in 3 varieties that were different harvesting stage and no report the amount of SOD in different parts of Jerusalem artichoke tuber was carried out. The aims of this study were to evaluate SOD activity in various varieties of Jerusalem artichoke and to evaluate the difference of SOD activity in different parts of Jerusalem artichoke tuber. This experiment used 3 varieties of Jerusalem artichoke including Hel 65, CN 52867 and JA 89 that were different genotypes. These tubers were separated into 3 parts: head, middle and tail for extraction and determination of SOD activity. The results showed that Jerusalem artichoke var. Hel 65 exhibited the highest SOD activity compared to var. CN 52867 and JA 89....
Tipo: PhysicalObject
Palavras-chave: Helainthus tuberosus; Jurusalem artichoke; Enzyme; Antioxidant enzyme; Superoxide dismutase; SOD; Genotypes; แก่นตะวัน; เยรูซาเลม อาร์ติโชค; การเปรียบเทียบพันธุ์; เอนไซม์; ซูเปอร์ออกไซด์ดิสมิวเทส; แอนติออกซิแดนท์.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5735

Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
Provedor de dados: 104
Autores: Aidar,Davi S.; Pagotto,Rubiane C.; Contel,Eucleia P.B.
Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona quadrifasciata anthidioides; Polymorphism; Electrophoresis; Enzyme; Stingless bees.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200018

Effect of enzyme addition on energy utilization and performance of broiler chickens fed wheat-based diet with different metabolizable energy levels
Provedor de dados: 2
Autores: Moftakharzadeh, Seyed adel; Janmohammadi, Hossein; Taghizadeh, Akbar; Kianfar, Ruhollah; Olyayee, Majid Geshlog.
An experiment was carried out to investigate the effect of multi-enzyme in high and low levels of metabolizable energy (13.81 and 11.51 MJ kg-1 diet) on performance and energy utilization of broilers fed wheat-soybean meal diets from 0 to 21 days of age. Result showed that birds fed diets containing 11.51 MJ kg-1 consumed significantly (p &lt; 0.05) more feed than diets containing 13.81 MJ kg-1, whereas daily gain and feed conversion ratio improved (p &lt; 0.05) when enzyme was added to 11.51 MJ kg-1 diet. There was significant improvement in metabolizable energy, net energy for production, organic and dry matter digestibility in 0-21 when diets supplemented with enzyme (p &lt; 0.05). Addition of enzyme to 11.51 MJ kg-1 containing diet significantly (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Net energy; Wheat; Body composition; Broilers.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/44585

Effect of different levels of supplementary alpha-amylase in finishing broilers
Provedor de dados: 2
Autores: Castro, Solange de Faria; Bertechini, Antonio Gilberto; Lima, Eduardo Machado Costa; Clemente, Alisson Hélio Sampaio; Ferreira, Verônica Gabriela Gonçalves; Carvalho, Julio Cesar Carrera de.
The objective of this study was to investigate the effects of alpha-amylase supplementation on performance and carcass and cuts yield in broilers during finishing phase (21 to 42 days). A completely randomized experimental design with six treatments and eight replications was used: Positive Control (PC): no enzyme; Negative control (NC): without enzyme formulated with 6.34% less energy (- 6.34% ME); NC1: with 250 g ton-1 of alpha-amylase; NC2: with 500 g ton-1 of alpha-amylase; NC3: with 750 g ton-1 of alpha-amylase; NC4: with 1000 g ton-1 of alpha-amylase. Significant differences in weight gain, feed intake and feed conversion were reported with the inclusion of alpha-amylase. The NC treatment with inclusion of amylase provided improvement values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Energy reduction; Enzyme; Performance.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/47546

Internal quality of laying hen eggs fed on protease at different storage and stocking conditions
Provedor de dados: 2
Autores: Suckeveris, Diana; Muñoz, Julian Andres; Demuner, Leandro Félix; Caetano, Vinícius Camargo; Faria Filho, Daniel Emydgio de; Faria, Douglas Emygdio de.
Effects on diets with reduction of crude protein and methionine + cystine (RCP) and protease supplementation (PRO) for laying hens at different ages and their influence on the internal quality of eggs at different conditions (STO) and storage times (TM) are evaluated. Four hundred and twenty eggs from commercial Hy-Line W36 laying hens collected at 32, 44 and 58 weeks of age were used. Experimental design was completely randomized in a factorial scheme 3 x 2 x 2 x 2: RCP (control, enhancement of one and two times the enzyme protease), PRO (with and without protease), STO (room temperature and refrigerated) and TM (14 and 28 days), with seven replications of one egg per experimental unit. The factorial scheme 3 x 2 x 2 was used at 58 weeks of age, because...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.04.00.00-2; 5.04.03.00-1; 5.04.03.01-0 egg yolk; Egg white; Enzyme; Haugh unit; Nutrition; Poultry.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/27929

Influence of xylanase and vitamin A in wheat-based diet on performance, nutrients digestibility, small intestinal morphology and digesta viscosity in broiler chickens
Provedor de dados: 2
Autores: Khoramabadi, Vahid; Akbari, Mohammad Reza; Khajali, Fariborz; Noorani, Hossein; Rahmatnejad, Enayat.
The effect of wheat-based diet (WBD), supplemented with xylanase and surplus vitamin A, on performance, nutrient digestibility, intestinal morphology and digesta viscosity of broiler chickens was investigated. Based on a completely randomized design, 240-day-old chickens were randomly divided into six experimental diets and four replications with 10 chickens each. Experimental diets consisted of corn-based diet (CBD) (T1), WBD with routine amounts of vitamin A (9000 IU kg-1) (T2), T2 without vitamin A in premix (T3), T2 + 6000 IU kg-1 vitamin A (T4), T2 + 420 IU kg-1 xylanase (T5), and T2 + 6000 IU kg-1 vitamin A + 420 IU kg-1 xylanase (T6). For Feed Conversion Rate (FCR) between day 1 and 21 and between day 21 and 42 WBD supplemented with vitamin A and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digestibility; Enzyme; Morphology; Viscosity; Vitamin A; Wheat.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/23910

Effect of phytase on digestibility of corn, sorghum and wheat bran by silver catfish (Rhamdia voulezi)
Provedor de dados: 2
Autores: Signor, Altevir; Lewandowski, Vanessa; da Silva, Rodrigo Aguiar; Fries, Edionei Maico; Schuller, Jean Marcel.
This study aimed to evaluate the digestibility of plant energetic ingredients, corn, sorghum and wheat bran, with and without phytase supplementation, in silver catfish (Rhamdia voulezi). The experimental design was completely randomized, represented by eight treatments, which consisted of the development of a reference diet and a test diet for each ingredient evaluated (corn, sorghum and wheat bran), with and without supplemental phytase. Diets were formulated so as to contain 70% reference feed and 30% test ingredient. The digestibility coefficients of dry matter and crude protein were significantly influenced only in relation to the evaluated ingredients. Phytase influenced the digestibility coefficients of energy and phosphorus, with interaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding; Enzyme; Plant energetic ingredients; Silver catfish.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/32054

OPTIMIZATION OF THE CULTURE CONDITIONS FOR TANNASE PRODUCTION BY Aspergillus sp. GM4 UNDER SOLID-STATE FERMENTATION
Provedor de dados: 3
Autores: Souza, Patrícia Nirlane da Costa; Maia, Natália da Costa; Guimarães, Luís Henrique Souza; Resende, Mário Lúcio Vilela de; Cardoso, Patrícia Gomes.
The production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF)  was investigated using different vegetables leaves such as mango, jamun, coffee and agricultural residues such as coffee husks, rice husks and wheat bran. Among substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. The best incubation time was studied and others variables were optimized using the Central Composite Design. The best conditions for tannase production were: incubation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Surface response methodology; Enzyme; Jamun; Agricultural residues.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/22731

Optimization of asparaginase production from Zymomonas mobilis by continuous fermentation
Provedor de dados: 3
Autores: Menegat, Francieli Bortoluzzi; Baldo, Cristiani; Celligoi, Maria Antonia Pedrine Colabone; Buzato, João Batista.
Asparaginase is an enzyme used in clinical treatments as a chemotherapeutic agent and in food technology to prevent acrylamide formation in fried and baked foods. Asparaginase is industrially produced by microorganisms, mainly gram-negative bacteria. Zymomonas mobilis is a Gram-negative bacterium that utilizes glucose, fructose and sucrose as carbon source and has been known for its efficiency in producing ethanol, sorbitol, levan, gluconic acid and has recently aroused interest for asparaginase production. Current assay optimizes the production of Z. mobilis asparaginase by continuous fermentation using response surface experimental design and methodology. The studied variables comprised sucrose, yeast extract and asparagine. Optimized condition obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sucrose; Asparagine; Yeast extract; Enzyme; Microbial production; Factorial design.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31057

Dhal Recovery From Enzyme Pretreated Pigeon Pea Cultivar Gjp1
Provedor de dados: 111
Autores: Dabhi, Mukesh N.; Sangani, V P; Rathod, P J.
GJP1 variety is newly developed variety of pigeon pea. It gives higher seed yield as compared to other varieties. Pigeon pea is mostly consumed in the form of splits hence, it is necessary to verify its hulling efficiency and utmost dhal recovery. Pre milling treatments are generally employed to loosen the seed coat to remove husk without losing any edible portion and better dhal recovery.  Xylanase, pectinase and cellulose enzymes were used to evaluate the milling properties of pigeon pea grains. Efforts were made to evaluate the effect of enzymatic parameters, i.e., enzyme concentration (20-50 mg/100g dry matter), incubation time (4-12 h), incubation temperature (35-55 oC) and tempering water pH (4.0-6.0) on hulling efficiency were optimized using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Processing; Milling; Enzyme Pigeon pea; Enzyme; Dhal recovery; Pretreatment; Hulling efficiency.
Ano: 2018
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4762

Inactivation of yeast inorganic pyrophosphatase by organic solvents
Provedor de dados: 42
Autores: Grazinoli-Garrido,Rodrigo; Sola-Penna,Mauro.
A number of application for enzymes in organic solvents have been developed in chemical processing, food related conversions and analyses. The only unsolved problem related to nonaqueous enzymology is the notion that enzymes in organic solvent are mostly far less active than in water. Therefore, studies concerning the mechanisms by which enzymes are inactivated by organic solvents would reveal a clear understanding of the structure-function relationship of this phenomenon. Here we analyzed the effects of a series of alcohols (methanol, ethanol, 1-propanol and 2-propanol) and acetone on the activity of yeast inorganic pyrophosphatase. We observed that solvents inactivated the enzyme in a dose-dependent manner. This inactivation is also dependent on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic solvents; Enzyme; Yeast inorganic pyrophosphatase; Alcohol.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400006

Expression of manganese peroxidase by Lentinula edodes and Lentinula boryana in solid state and submerged system fermentation
Provedor de dados: 42
Autores: HERMANN,KATIA L.; COSTA,ALESSANDRA; HELM,CRISTIANE V.; LIMA,EDSON A. DE; TAVARES,LORENA B.B.
The production of ethanol from lignocellulosic biomass is referred as a second generation biofuel, whose processing is one of the most promising technologies under development. There are few available studies on the use of enzymes produced by fungi as active for the biodegradation of lignocellulosic biomass. However, the manganese peroxidase (MnP) enzyme presents high potential to degrade lignin and the basidiomycetes are the major producers of this oxidase. Thus, this study aimed at evaluating the ability of fungi Lentinula edodes and Lentinula boryana to produce this enzyme when cultivated in submerged fermentation system (SS) and also in solid-state fermentation system (SSF) containing Eucalyptus benthamii sawdust with or without corn cob meal. In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basidiomycetes; Eucalyptus benthamii; Enzyme; Ethanol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300965

Growth performance and carcass yield of broiler chickens in response to carbohydrases and its association with phytase
Provedor de dados: 45
Autores: Dessimoni,G.V.; Sakomura,N.K.; Donato,D.C.Z.; Soares,L.; Sarcinelli,M.F.; Malheiros,E.B.; Dalólio,F.S.
ABSTRACT An experiment was conducted to evaluate broiler chicken performance and carcass yield in response to carbohydrase supplementation (Xylanase and Betaglucanase; XB) and association of carbohydrase and phytase (PHY). A total of 1,920 day-old male broiler chicks (Cobb 500) were distributed in a completely randomized design with 8 treatments and 8 replicates each, allocated in 64 pens with 30 chicks each. The treatments were: Positive Control (PC); Negative Control (NC): reduced ME [-120kcal/kg (Starter) and -170kcal/kg (Finisher)]; NC + XB [50g/ton]; NC + XB [100g/ton]; NC + XB [150g/ton]; NC + XB [50g/ton] + PHY [100g/ton]; NC + XB [100g/ton] + PHY [100g/ton]; NC + XB [150g/ton] + PHY [100g/ton]. The inclusion of XB (150) and XB (50, 100, and 150)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additives; Corn; Metabolizable energy; Enzyme; Soybean meal.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300983

Comparisons Between Different Methods in Measuring Enzyme Similarity for Metabolic Network Alignment
Provedor de dados: 52
Autores: Zhou,Wenwei; Liu,Jiangtao; Xing,Hiu; Zhang,Zhengdong; Xie,Xiaoyao; Jing,Fengxuan.
Metabolic network alignments enable comparison of the similarities and differences between pathways in two metabolic networks and help to uncover the conserved sub-blocks therein. Such analysis is important in the understanding of metabolic networks and species evolution. The fundamental parts of metabolic network alignment algorithms all involve comparisons of the similarity between two enzymes as a similarity measure of network nodes. As a result, the study of methods for measuring enzyme similarity becomes highly relevant. Currently, two approaches are mainly used to measure enzyme similarity. One of the methods is based on similarity measures of gene or protein sequences; the other is based on enzyme classification. In this study, multiple metabolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic network alignment; Enzyme; Similarity measure; Classification numbering; Sequence similarity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100405

Production of tannase by Aspergillus tamarii in submerged cultures
Provedor de dados: 52
Autores: Costa,Andréa M.; Ribeiro,Wanessa X.; Kato,Elaine; Monteiro,Antonio Roberto G.; Peralta,Rosane Marina.
The production of tannase by Aspergillus tamarii was studied in submerged cultures. The fungus produced an extracellular tannase after two days of growth in mineral medium containing tannic acid, gallic acid and methyl gallate as carbon source. The best result was obtained using gallic acid as inducer (20.6 U/ml). The production of enzyme was strongly repressed by the presence of glucose. Crude enzyme was optimally active at pH 5.0 and 30º C. The enzyme was stable in a large range of pH and up to the temperature of 45º C.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus tamarii; Enzyme; Fungal biomass; Submerged cultures; Tannase; Tannins.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200021

Multivariable parameter optimization for the endoglucanase production by Trichoderma reesei Rut C30 from Ocimum gratissimum seed
Provedor de dados: 52
Autores: Das,Mithu; Banerjee,Rintu; Bal,Satish.
The aim of this study was to evaluate the interaction effects of the physico-chemical parameters on the endoglucanase (CMCase) production by Trichoderma reesei Rut C30 on a cellulosic agro-residue by the solid-state fermentation (SSF) and to determine their optimum values by the EVOP factorial design technique. The best combination of physical parameters for the maximum production of the endoglucanase (CMCase) was 28ºC temperature, 79% relative humidity and 4.8 pH of the medium. The best combination of the chemical parameters was (mg/L) nicotinic acid 15, naphthalene acetic acid 7, ferric chloride 5 and Tween-80 6. With the application of this technique, the yield of the CMCase increased by ~ 2.3 fold.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Endoglucanase; Carboxymethylcellulase (CMCase); EVOP factorial design technique.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100005

Co-production of Proteases and Bioactive Protein Hydrolysates from Bioprocessing of Feather Meal
Provedor de dados: 52
Autores: Bernardo,Bernardete da Silva; Ramos,Rodrigo Ferraz; Callegaro,Kelly; Daroit,Daniel Joner.
Abstract: Feather meal conversion through submerged cultivations with Bacillus strains (CL33A, CL14) yielded proteases and protein hydrolysates. After 4-day (CL33A) and 10-day (CL14) cultivations, protease activities reached 461 U/mL; hydrolysates presented antioxidant (radicals-scavenging, 57-77%; Fe2+-chelation, 14-28%; Fe3+-reduction) and antidiabetic (dipeptidyl peptidase-IV inhibition, 49-52%) potentials. The obtained bioproducts present prospective commercial/industrial applications.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus; Bioprocess; Enzyme; Antioxidant; Antidiabetic.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100220

Acid and enzymatic hydrolysis to recover reducing sugars from cassava bagasse: an economic study
Provedor de dados: 52
Autores: Woiciechowski,Adenise Lorenci; Nitsche,Saul; Pandey,Ashok; Soccol,Carlos Ricardo.
The objective of this work was to study the acid and enzymatic hydrolysis of cassava bagasse for the recovery of reducing sugars and to establish the operational costs. A statistical program "Statistica", based on the surface response was used to optimize the recovery of reducing sugars in both the processes. The process economics was determined considering the values of reducing sugars obtained at laboratory scale, and the operations costs of a cylindrical reactor of 1500 L, with flat walls at the top and bottom. The reactor was operated with 150 kg of cassava bagasse and 1350 kg of water. The yield of the acid hydrolysis was 62.4 g of reducing sugars from 100 g of cassava bagasse containing 66% starch. It represented 94.5% of reducing sugar recovery. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava bagasse; Hydrolysis; Acid; Enzyme; Reducing sugars; Economics.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300018

Purification and characterisation of trypsin-like enzyme from the pyloric caeca of cod (Gadus morhua) II
Provedor de dados: 52
Autores: Beirão,Luiz Henrique; Mackie,Ian Mckintoch; Teixeira,Evanilda; Damian,César.
A trypsin -like enzyme from the pyloric caeca of cod (Gadus morhua) was purified by affinity chromatography on CHOM Sepharose 4B. Some characteristics were established by its catalytic activity on T.A.M.E., typical enzyme substrate, and serine protease inhibitors. The enzyme had an isoelectric point of 5.30 and 5.89 and was very similar in amino acid composition to bovine trypsin, but differed in having a higher relative amount of acidic amino acids and a lower amount of basic amino acids. The enzyme also hydrolysed fish protein substrates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cod; Enzyme; Trypsin.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100005

Chromatographic characterization of products isolated from chrome shavings
Provedor de dados: 52
Autores: Garcia,Edivaldo E; Kimura,Cláudia C.M; Martins,Ariovaldo C; Rocha,Gisele O; Nozaki,Jorge.
Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chrome shavings; Enzyme; Chromatography; Protein; Chromium.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300003

Use of vinasse and sugarcane bagasse for the production of enzymes by lignocellulolytic fungi
Provedor de dados: 52
Autores: Aguiar,Mario Mamede; Ferreira,Luiz Fernando Romanholo; Monteiro,Regina Teresa Rosim.
In this present work, three strains of Pleurotus and Trichoderma reesei were cultivated in media with pre-treated bagasse and vinasse. Cellulolytic and lignolytic activities and biomass production were analyzed. The treatment of the bagasse with 2% H2O2 + 1.5% NaOH + autoclave resulted in a greater fiber breakage increasing the cellulose level up to 1.2 times and decreasing 8.5 times the hemicellulose content. This treatment also resulted in a high lignolytic activity for all cultures utilized. T. reesei produced laccase, peroxidase and manganese-peroxidase in all the treatments, having its manganese-peroxidase activity raging from 1.9 to 4.8 times higher than the basidiomycetes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bagasse; Enzyme; Fungi; Lignocellulolytic; Pleurotus; Sugarcane.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500030

Production and characterization of galactose oxidase produced by four isolates of Fusarium graminearum
Provedor de dados: 52
Autores: Gasparotto,Elainy Patrícia Lino; Abrão,Sorrailla Carolina Carrard; Inagaki,Suely Yaeko; Tessmann,Dauri José; Kemmelmeier,Carlos; Tessmann,Ione Parra Barbosa.
A screening aimed to find new galactose oxidase producer isolates and to evaluate the production among Fusarium graminearum strains was conducted. Thirty-five isolates out of 39 analysed produced the enzyme at several levels. The data indicated a wide distribution of galactose oxidase within F. graminearum and also revealed new producer isolates. The enzyme produced by different isolates showed similar thermal activity and stability and were active on same substrates. However, the optimum pH ranged from 7.0 to 7.5. Thus, all evaluated isolates were suitable for the production of galactose oxidase.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galactose oxidase; Fusarium graminearum; Enzyme.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500004

Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers
Provedor de dados: 56
Autores: Fonseca,A.S.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.
Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T4endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Enzyme; Escherichia coli; Filamentation; Laser.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000011

Carbon sources effect on pectinase production from Aspergillus japonicus 586
Provedor de dados: 58
Autores: Teixeira,Maria F. S.; Lima Filho,José L.; Durán,Nelson.
The effect of different carbon sources on the pectinesterases, endo- and exo-polygalacturonase activities from Aspergillus japonicus 586 was evaluated in liquid media (Manachini solutions) supplemented with different substrate concentrations. The culture medium was inoculated with 5.10(6) spores/ml and mantained under agitation (140 rpm), at 30°C, during 122 h. The enzyme evaluation was carried out 24 h after filtration. The crude extract from A. japonicus 586 indicated that the best enzymatic activities were afforded in the presence of 0.5% pectin (pectinesterease), 0.2% pectin and 0.2% glycerol (endopolygalacturonase), and 0.5% pectin associated to 0.5% glucose (exopolygalacturonase). Carbon sources concentration, isolated or associated, significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pectinases; Aspergillus japonicus; Enzyme; Galacturonase; Pectinesterases.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400009

Laccase production by Lepista sordida
Provedor de dados: 58
Autores: Cavallazzi,José Renato Pereira; Oliveira,Maria Goreti de Almeida; Kasuya,Maria Catarina Megumi.
A Lepista sordida laccase has been characterized. Laccase and manganese peroxidase were detected in liquid medium with ammonium phosphate, yeast extract and ammonium molybdidate as nitrogen sources after 3 days of cultivation. Laccase optimal temperature and pH were 45ºC and 3.5, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lepista sordida; Laccase; Enzyme.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200016

Comparative study on production of α-Amylase from Bacillus licheniformis strains
Provedor de dados: 58
Autores: Divakaran,Dibu; Chandran,Aswathi; Pratap Chandran,R.
Alpha amylase (α-1, 4-glucan-glucanhydrolase, EC 3.2.1.1), an extracellular enzyme, degrades α, 1-4 glucosidic linkages of starch and related substrates in an endo-fashion producing oligosaccharides including maltose, glucose and alpha limit dextrin (7). The present study deals with the production and comparative study of production of α-amylase from two strains of Bacillus licheniformis, MTCC 2617 and 2618, by using four different substrates, starch, rice, wheat and ragi powder as carbon source by submerged fermentation. The effect of varying pH and incubation temperature, activator, inhibitor, and substrate concentration was investigated on the activity of α-amylase produced by MTCC strain 2618. The results shows that the production of the α-amylase by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylase; Bacillus licheniformis; Enzyme; Extracellular; Glucose.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400022

Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68
Provedor de dados: 58
Autores: Aygan,Ashabil; Arikan,Burhan; Korkmaz,Hatice; Dinçer,Sadik; Çolak,Ömer.
An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25ºC and 40ºC. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20ºC and 90ºC, with an optimum at 50ºC; and maximum activity was at pH 10.5. The enzyme was almost completely stable up to 80ºC with a remaining activity over 90% after 30 min pre-incubation. Thermostability was not increased in the presence of Ca2+. An average of 75% and 60ºC of remaining activity was observed when the enzyme was incubated between pH 5 and 9 for 1 h and for 2 h, respectively. The activity of the enzyme was inhibited by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus sp.; Α-amylase; Alkaliphilic; Thermostable; Enzyme.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027

Bioremediation of polyaromatic hydrocarbons (PAHs) using rhizosphere technology
Provedor de dados: 58
Autores: Bisht,Sandeep; Pandey,Piyush; Bhargava,Bhavya; Sharma,Shivesh; Kumar,Vivek; Sharma,Krishan D.
The remediation of polluted sites has become a priority for society because of increase in quality of life standards and the awareness of environmental issues. Over the past few decades there has been avid interest in developing in situ strategies for remediation of environmental contaminants, because of the high economic cost of physicochemical strategies, the biological tools for remediation of these persistent pollutants is the better option. Major foci have been considered on persistent organic chemicals i.e.polyaromatic hydrocarbons (PAHs) due to their ubiquitous occurrence, recalcitrance, bioaccumulation potential and carcinogenic activity. Rhizoremediation, a specific type of phytoremediation that involves both plants and their associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PAH; Bioremediation; Rhizoremediation; Enzyme; Plant microbe pair.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100007

Estimating the turnover number in enzyme kinetic reactions using transient and stationary state data
Provedor de dados: 60
Autores: Uludag-Demirer,Sibel; Duran,Jorge; Tanner,Robert D.
Substrate and product concentration data obtained by simulating enzyme-substrate reaction rate equations were used to test two proposed kinetic rate constant estimation techniques in this study. In the first technique, the turnover number, k3, was calculated using early transient time domain data, which are difficult to obtain experimentally. The technique used an iterative approach to calculate k3 with a pair of data and the value of k3 could be retrieved with 35% error. The second technique calculated k3 using stationary domain data and the value of k3 could be retrieved with less than 5% error. This second technique also offered internal consistency in the calculation of k3 by calculating k3 both from the intercept and the slope of the linear plot...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Kinetics/rate constant; Enzyme reactions.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400005

Activity of fungal phytases stored in two ways in response to the period of storage at room temperature
Provedor de dados: 65
Autores: Naves,Luciana de Paula; Corrêa,Angelita Duarte; Bertechini,Antônio Gilberto; Gomide,Elisangela Minati; Abreu,Celeste Maria Patto de.
Two distinct experiments were conducted simultaneously with phytases of Aspergillus oryzae and A. niger for determining enzyme activity in response to storage period (up to 180 days at room temperature - RT). In the first experiment, enzymes were stored as they were acquired (pure form) and the activity was measured periodically during 180 days of storage at RT. In the second experiment, the phytases were incorporated to a supplement containing vitamins, minerals, and amino acids; and then this supplement was stored at RT up to 180 days, so that every 30 days of storage was collected one aliquot from each replicate for determining enzymatic activity. In conclusion, the phytase activity is affected by storage duration. To ensure 80% of the initial activity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus niger; Aspergillus oryzae; Enzyme; Phytate; Phytic acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000100029

Phytate-phosphorus and phytase contents on the relative weight of organs, intestinal morphometry and performance of broilers
Provedor de dados: 65
Autores: Catalan,Aiane Aparecida da Silva; Krabbe,Everton Luis; Avila,Valdir Silveira de; Lopes,Letícia dos Santos; Nunes,Ana Paula; Morés,Marcos Antonio Zanella; Roll,Victor Fernando Büttow; Xavier,Eduardo Gonçalves.
ABSTRACT: In order to evaluate the efficiency of phytase in diets with low and high phytate phosphorus (PP) 0content, as a consequence of wheat bran inclusion, on the relative weight of organs, intestinal morphometry and performance, three hundred and eighty-four male Cobb500 broilers were housed in metabolic cages. Animals were assigned into four treatments in a 2x2 factorial scheme in a randomized block design with eight replicates of 12 birds each. From 11 days of age birds received experimental diets, which consisted of: Diet low in PP; Diet low in PP with phytase (500FTU kg-1); Diet with a high PP and Diet with a high PP with phytase (500FTU kg-1). At 22 and 32 days of age two birds were slaughtered in order to collect gizzard, heart, liver, cecum,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bird; Enzyme; Substrate; Wheat bran.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001858

Increasing levels of phytase in diets formulated with reduced available phosphorus content supplied to male and female broilers
Provedor de dados: 65
Autores: Naves,Luciana de Paula; Rodrigues,Paulo Borges; Teixeira,Levy do Vale; Bertechini,Antônio Gilberto; Alvarenga,Renata Ribeiro; Bernardino,Verônica Maria Pereira; Santos,Luziane Moreira dos; Natividade,Yuri Pereira Efrem.
The reduction of the available phosphorus (avP) content in the broiler diet must be followed by the supplementation of adequate phytase. One experiment was conducted with broilers from 15 to 28 days of age to determine the level of necessary phytase when the mashed diet is formulated with 0.213% of avP to enable the results of performance, tibia ash content, and calcium (Ca) utilization similar to those determined for birds fed with diet formulated to meet their nutritional requirements. Thus, 120 broilers were distributed in (4+1) x2 factorial arrangement corresponding to four deficient diets in avP (0.213%) supplemented with phytase (0; 750; 1,500 or 2,250FTU kg-1) plus one positive control diet without phytase (0.426% of avP), supplied to male and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aviculture; Bone ash; Enzyme; Metabolism; Performance; Phytate.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801479

Nutritional and energetic value of rice by-products, with or without phytase, for growing pigs
Provedor de dados: 65
Autores: Dadalt,Julio Cezar; Ribeiro,Andréa Machado Leal; Kessler,Alexandre de Mello; Wesendonck,William Rui; Bockor,Luciane; Gomes,Gilson Alexandre.
The objective of this study was to evaluate nutritional and energetic value of rice by-products, with or without phytase, using growing pigs. Thirty-six male pigs were housed in individual metabolic cages. Total collection of feces and urine was carried out in two periods of ten days: five days for adaptation and five days for collection. A randomized blocks design was used, considering the sampling period as a block, with five treatments and seven replicates. Two control diets (with and without phytase - Phy) were used in the digestibility calculations, the latter in order to evaluate the enzyme influence on energy digestibility of the tested ingredients. The control diet was replaced by 30% of one of the ingredients: defatted rice bran (DRB) with and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Alternative ingredients; Dephytinization; Enzyme; Nutrients.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701305

Carcass characteristics of broilers at 42 days receiving diets with phytase in different energy and crude protein levels
Provedor de dados: 64
Autores: Nagata,Adriano Kaneo; Rodrigues,Paulo Borges; Alvarenga,Renata Ribeiro; Zangeronimo,Márcio Gilberto; Donato,Daniella Carolina Zanardo; Silva,José Humberto Vilar da.
The aim of this study was to evaluate carcass characteristics and quality of breast meat after the inclusion of phytase in broiler diets with different levels of apparent metabolizable energy (AMEn) corrected for nitrogen and reduced crude protein (CP) supplemented with essential amino acids following the concept of ideal protein. A total of 1,500 Cobb broilers from 22 to 42 days (initial weight of 833 ± 7 g and final weight of 2741 ± 48 g) were distributed in completely randomized design in a factorial 3x3+1 experiment (three levels of AMEn - 2,950; 3,100 and 3,250 kcal/kg - and three levels of CP - 14, 16 and 18% - and an additional treatment - control without phytase, with 3,100 kcal/kg EMAn, 19.2% PB and 0.4% available phosphorus), in six replications...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Available phosphorus; Dietary addictive; Enzyme; Ideal protein.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300020

Phytase in rations of growing pigs: performance, blood parameters and bone mineral content
Provedor de dados: 64
Autores: Silva,Hunaldo Oliveira; Fialho,Elias Tadeu; Freitas,Rilke Tadeu Fonseca de; Lima,José Augusto de Freitas; Logato,Priscila Rosa Vieira; Schoulten,Neudi Artemio.
It was intended with the present work to verify the effect of phytase on the performance, bone mineralization and content of plasma urea of swine. 80 swine (30 ± 3.1 kg/LW), crossbred (LD x LW) males and females, allocated to a randomized block design (RBD) with four treatments and five replicates. The treatments consisted of a growing swine diet on the basis of corn, soybean meal and defatted rice bran (DRB) supplemented with four levels of phytase (0, 400, 800 and 1200 FTU/kg) The diet was formulated to meet the requirements of growing swine except for available calcium and phosphorus, the levels of which were 0.64 and 0.25%, respectively. The enzyme phytase utilized was Natuphos 5000. Over the experimental period every eight days, blood of two animals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Minerals; Plasma; Urea.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000600028

Nitrogen, calcium and phosphorus balance of broilers fed diets with phytase and crystalline amino acids
Provedor de dados: 64
Autores: Gomide,Elisangela Minati; Rodrigues,Paulo Borges; Zangeronimo,Márcio Gilberto; Bertechini,Antônio Gilberto; Santos,Luziane Moreira dos; Alvarenga,Renata Ribeiro.
The objective of this study was to evaluate the effect of reduced-crude protein (CP) and available phosphorus (aP) diets, added with phytase and amino acids for broilers in the growing (14 to 21 days old) and retirement (35 to 42 days) phases on nitrogen, potassium and phosphorus balance. Ninety Cobb broilers were distributed in a completely randomized design with three treatments and six replications with five and three birds per experimental unit in the growing and retirement phases, respectively. Diets were elaborated to contain reduced CP and aP levels (19 and 17% CP in the growing phase, with 0.34% aP; and 18 and 16% CP in the retirement phase, with 0.30% aP), supplemented with crystalline amino acids and 500 FTU of phytase/kg diet. These were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Phytate; Environment; Metabolism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300022