Consulta: creatorFacets:"五十嵐, 郁男"
Registros recuperados: 66
Data/hora: 11/06/2024 03:29:37

全共　帯広畜産大学原虫病研究センター
Provedor de dados: 19
Autores: 五十嵐, 郁男.
Palavras-chave: 原虫病; 診断; 予防.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1740

BABESIA CABALLI : ANALYSIS OF CHROMOSOMES SEPARATED BY PULSE FIELD GEL ELECTROPHORESIS
Provedor de dados: 19
Autores: Ikadai, Hiromi; Kudo, Noboru; Oyamada, Takashi; Igarashi, Ikuo; 五十嵐, 郁男.
Palavras-chave: Babesia caballi; Pulsed field gel electrophoresis; Chromosome; Rhoptry gene.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/642

The Babesia bovis BvP36 gene is expressed at least in the asexual stage of Babesia merozoite
Provedor de dados: 19
Autores: Khukhuu, A; Sivakumar, T; Alhassan, A; Ota, N; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男.
Recently, Babesia bovis genome project identified a novel gene, which is homologous to the gene encoding sporozoite P36 antigen in Plasmodium parasites. In the present study, we isolated the P36 homologous gene from the genomic DNA of B. bovis and designated it as the BvP36 gene. The sequence of the BvP36 gene was identical to that reported previously. This gene was expressed in an Escherichia coli system to produce a recombinant protein (rBvP36). The rBvP36 protein was produced as a highly soluble form in E. coli and was detected by western blot analyses of sera collected from cattle experimentally infected with B. bovis and B. bigemina merozoites. In indirect immunofluorescence tests, sera collected from mice immunized with rBvP36 showed strong reactions...
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2385

バベシア原虫感染防御抗原の発生工学的解析
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
1997年度～2000年度科学研究費補助金基盤研究(B)(2)研究成果報告書09460143
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2285

Toxoplasma Lysate Antigen (TLA144)-Ig8 Antigenic Component : Binding and Induction of Cytotoxic Cell Activity in Mouse Spleen Cells
Provedor de dados: 19
Autores: Kato, Mihoko; Maki, Yoshiyuki; Inoue, Noboru; Omata, Yoshitaka; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Cytotoxic cell activity; NK cell; LAK cell; Toxoplasma lysate antigen.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/216

実験的バベシア病の感染防御における細胞性免疫の検討
Provedor de dados: 19
Autores: 五十嵐, 郁男.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/528

夢のワクチン・自殺原虫ワクチン開発構想
Provedor de dados: 19
Autores: 五十嵐, 郁男.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/853

TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS
Provedor de dados: 19
Autores: Fujii, Kei; Yokoyama, Naoaki; Takabatake, Noriyuki; Suzuki, Hiroshi; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 鈴木, 宏志; 玄, 学南; 五十嵐, 郁男.
In hemoprotozoa，gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study，a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection，clear positive fluorescences were detected in some parasite bodies， indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However， the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that...
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/631

Molecular and Immunological Characterization of a Novel 32-kDa Secreted Protein of Babesia microti
Provedor de dados: 19
Autores: Ooka, Hideo; Terkawi, Mohamad A; Cao, Shinuo; Aboge, Gabriel; Goo, Youn-Kyoung; Luo, Yuzi; Li, Yan; Nishikawa, Yoshifumi; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
A cDNA encoding the Babesia microti 32-kDa protein was identified by serological immunoscreening of a cDNA expression library and designated as BmP32. The full length of BmP32 contains an open reading frame of 918 base pairs consisting of 306 amino acids having a significant homology with B. microti secreted antigen 1. Antiserum raised against recombinant protein (rBmP32) specifically reacted with a 32-kDa native protein of the parasite lysate using western blot analysis. The indirect immunofluorescent antibody test showed a preferable localization of BmP32 in the cytoplasm of the intra- and extracellular parasites. Moreover, BmP32 was secreted in the cytosol of infected erythrocytes, especially during the peak parasitemia and the recovery phase of the...
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3820

IN VITRO GROWTH INHIBITORY EFFECTS OF COMBINED APPLICATIONS OF CLOTRIMAZOLE, KETOCONAZOLE, AND CLODINAFOP-PROPARGYL ON EQUINE BABESIA PARASITES
Provedor de dados: 19
Autores: Bork, Sabine; Yokoyama, Naoaki; Okamura, Masashi; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Recently，we reported the in vitro growth inhibitory effects of the imidazole derivatives，clotrimazole(CLT) and ketoconazole (KC)，and the herbicide，clodinafop-propargyl(CP)，against bovine and equine Babesia parasites. Additionally，in the bovine species，several combined applications of the CLT，KC，and CP were reported to significantly increase the inhibitory effects. In this study，similar combination tests were carried out in order to confirm possible synergistic effects in the equine species，Babesia caballi and Babesia equi. Combinations of CLT/KC， CLT/CP，and CLT/KC/CP exerted a significantly enhanced growth inhibitory efficacy in B. caballi，in contrast to B. equi，where no synergistic effects were observed in any of the combinations. Our results suggest that...
Palavras-chave: In vitro; Babesia; Clotrimazole; Ketoconazole; Clodinafop-propargyl.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/637

Different Activation of Lymphocytes in Reaction to Babesia microti Infection in iNOS-/- and Wildtype Mice
Provedor de dados: 19
Autores: Remer, Katharina; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行.
Palavras-chave: Lymphocytes; Babesia microti; Spleen; INOS-/- mice; CD4+ cells; CD8+ cells; IFN-γ; Antibody-titer.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/263

Characterization of P15 Antigen of Cryptosporidium parvum Expressed by a Recombinant Vaccinia Virus
Provedor de dados: 19
Autores: Xuan, Xuenan; Zhang, Sofa; Kamio, Tsugihiko; Tsushima, Y.; Kamada, Takenori; Nishikawa, Yoshifumi; Otsuka, Haruki; Karanis, Panagiotis; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; 玄, 学南; 西川, 義文; 五十嵐, 郁男.
Palavras-chave: C. parvum; P15; Vaccinia virus; Subunit vaccine.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/314

Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction
Provedor de dados: 19
Autores: Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi,  and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction（PCR）method．The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001％. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281

Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis
Provedor de dados: 19
Autores: Jia, Honglin; Zhou, Jinlin; Ikadai, Hiromi; Matsuu, Aya; Suzuki, Hiroshi; Fujisaki, Kozo; Xuan, Xuenan; 鈴木, 宏志; 五十嵐, 郁男; 玄, 学南.
Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These...
Palavras-chave: Babesia gibsoni; Secreted Antigen 1; Identification; Serodiagnosis.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1655

Production of Transgenic Mice Carrying p30 Gene Encoding Major Surface Antigen of Toxoplasma gondii
Provedor de dados: 19
Autores: Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行.
Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254

Molecular Prevalence of Different Genotypes of Theileria orientalis Detected from Cattle and Water Buffaloes In Thailand
Provedor de dados: 19
Autores: Altangerel, Khukhuu; Sivakumar, Thillaiampalam; Inpankaew, Tawin; Jittapalapong, Sathaporn; Terkawi, Mohamad Alaa; Ueno, Akio; Xuan, Xuenan; Igarashi, Ikuo; Yokoyama, Naoaki; 玄, 学南; 五十嵐, 郁男; 横山, 直明.
Here we report on an epidemiological study regarding the molecular prevalence of different genotypes of Theileria orientalis present among domestic cattle and water buffalo populations bred in Thailand. A phylogenetic analysis based on the parasitic gene encoding a major piroplasm surface protein revealed the presence of 5 genotypes (Types 1, 3, 5, 7, and N-3) in cattle and 7 genotypes (Types 1, 3, 4, 5, 7, N-2, and N-3) in water buffaloes. Types 4, 7, and N-3 of T. orientalis were reported for the first time in water buffaloes. The previously reported C and Thai types from Thailand clustered as types 7 and 6, respectively, in the present analysis. Great similarities were observed among nucleotide sequences of isolates of the same genotype from cattle and...
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3592

The Genetic Diversity of Merozoite Surface Antigen 1 (MSA-1) among Babesia bovis Detected from Cattle Populations in Thailand, Brazil and Ghana
Provedor de dados: 19
Autores: NAGANO, Daisuke; SIVAKUMAR, Thillaiampalam; DE DE MACEDO, Alane Caine Costa; INPANKAEW, Tawin; ALHASSAN, Andy; IGARASHI, Ikuo; YOKOYAMA, Naoaki; 五十嵐, 郁男; 横山, 直明.
In the present study, we screened blood DNA samples obtained from cattle bred in Brazil (n=164) and Ghana (n=80) for Babesia bovis using a diagnostic PCR assay and found prevalences of 14.6% and 46.3%, respectively. Subsequently, the genetic diversity of B. bovis in Thailand, Brazil and Ghana was analyzed, based on the DNA sequence of merozoite surface antigen-1 (MSA-1). In Thailand, MSA-1 sequences were relatively conserved and found in a single clade of the phylogram, while Brazilian MSA-1 sequences showed high genetic diversity and were dispersed across three different clades. In contrast, the sequences from Ghanaian samples were detected in two different clades, one of which contained only a single Ghanaian sequence. The identities among the MSA-1...
Palavras-chave: Babesia bovis; Brazil; Ghana; MSA-1; Thailand.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4014

馬が決めた私の人生、ウマピロプラズマ病の診断法の開発
Provedor de dados: 19
Autores: 五十嵐, 郁男.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4018

原虫病診断技術の開発
Provedor de dados: 19
Autores: 五十嵐, 郁男; 横山, 直明; 井上, 昇; 玄, 学南.
発行年は推定
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3515

帯広畜産大学原虫病研究センター
Provedor de dados: 19
Autores: 五十嵐, 郁男.
研究機関の紹介(5)
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2999

Babesia bovis Dihydroorotate Dehydrogenase (BboDHODH) is a Novel Molecular Target of Drug for Bovine Babesiosis
Provedor de dados: 19
Autores: KAMYINGKIRD, Ketsarin; CAO, Shinuo; MASATANI, Tatsunori; MOUMOUNI, Paul Franck Adjou; VUDRIKO, Patrick; MOUSA, Ahmed Abd El Moniem; TERKAWI, Mohamad Alaa; NISHIKAWA, Yoshifumi; IGARASHI, Ikuo; XUAN, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
The emergence of drug resistance and adverse side effects of current bovine babesiosis treatment suggest that the search of new drug targets and development of safer and effective compounds are required. This study focuses on dihydroorotate dehydrogenase (DHODH), the fourth enzyme of pyrimidine biosynthesis pathway as a potential drug target for bovine babesiosis. Recombinant Babesia bovis DHODH protein (rBboDHODH) was produced in Escherichia coli and used for characterization and measurement of enzymatic activity. Furthermore, the effects of DHODH inhibitors were evaluated in vitro. The recombinant B. bovis DHODH histidine fusion protein (rBboDHODH) had 42.4-kDa molecular weight and exhibited a specific activity of 475.7 ± 245 Unit/mg, a Km = 276.2 µM for...
Palavras-chave: Atovaquone; Babesia bovis; Chemotherapeutic target agent; Dihydroorotate dehydrogenase; Leflunomide.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3918

重要動物原虫症を一滴の血液で網羅的に診断可能なペプチドアレイの開発
Provedor de dados: 19
Autores: 五十嵐, 郁男; IGARASHI, IKUO.
2006年度～2009年度科学研究費補助金基盤研究(B) 研究成果報告書18380181
Palavras-chave: 重要原虫病; 網羅的; 診断; ペプチド(プロテイン)アレイ; 血清; 少量試料.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2762

Role of CD4⁺ T Cells in the Control of Primary Infection with Babesia microti in Mice
Provedor de dados: 19
Autores: Igarashi, Ikuo; Waki, Seiji; Ito, Mamoru; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; 五十嵐, 郁男.
Palavras-chave: Babesia microti; Nude mice; CD4⁺ T Cells; Primary infection; Gamma interferon.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/218

In vitro inhibitory effect of fosmidomycin on the asexual growth of Babesia bovis and Babesia bigemina
Provedor de dados: 19
Autores: Sivakumar, T; Aboulaila, M. R. A.; Khukhuu, A; Iseki, H; Alhassan, A; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
The in vitro inhibitory effect of fosmidomycin was evaluated against the asexual growth of bovine Babesia parasites (Babesia bovis and Babesia bigemina). In the cultures with these parasites, severe reduction of parasite growth was observed by the addition of 1.25 μg/ml of the final drug concentration. Under these conditions, B. bigemina was completely cleared until the third day of cultivation. On the other hand, B. bovis was completely eliminated on the fourth day when the concentration was 6.25 μg/ml in the culture. These parasites failed to grow again when a drug-free medium was substituted for the subsequent cultivation. The IC50 values of fosmidomycin against B. bovis were determined as 0.88 and 0.63 μg/ml in the in vitro cultures with...
Palavras-chave: Fosmidomycin; Babesia bovis; Babesia bigemina; Asexual growth; In vitro culture.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2235

Prevalence of the enteric parasites of ducks from Behera governorate, Egypt
Provedor de dados: 19
Autores: AbouLaila, M; El-Bahy, N; Hilali, M; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男.
This study of enteric parasites of ducks was undertaken to determine their prevalence and the relationships between their prevalence and the age and breed of ducks in the Behera governorate, Egypt. A total of 110 ducks of different ages and breeds were examined. The prevalence of helminths was 4.54% from which the prevalence of nematode, cestode, and trematode infections was 2.72%, 3.63%, and 1.81%, respectively. The recovered nematodes were Ascaridia galli (0.9%) and Heterakis gallinarum (1.81%). The reported trematodes were Echinoparyphium recurvatum (0.9%) and Echinoparyphium paraulum (1.81%). The new reported cestodes were Cladogynia phoeniconaiadis (3.63%), Echinolepis carioca (3.63%), and Baerfainia anoplocephaloides (3.63%). The helminths infections...
Palavras-chave: Ducks; Enteric parasites; Egypt.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3125

Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle
Provedor de dados: 19
Autores: Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%)....
Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142

CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS
Provedor de dados: 19
Autores: Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624

IMMUNOSCREENING OF BABESIA RODHAINI GENES ENCODING IMMUNOGENIC PROTEINS FROM THE CDNA EXPRESSION PHAGE LIBRARY
Provedor de dados: 19
Autores: Fujii, Kei; Takabatake, Noriyuki; Boonchit, Suthisak; Hirata, Haruyuki; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Palavras-chave: Babesia rodhaini; Babesiosis; Immunoscreening; CDNA expression library.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/626

Phylogenetic analysis of Theileria orientalis in cattle bred in Fujian province，China
Provedor de dados: 19
Autores: Sivakumar, T; Khukhuu, A; Igarashi, I; Xuan, X; Guo, G; Huang, X; Yokoyama, N; 五十嵐, 郁男; 玄, 学南; 横山, 直明.
A molecular epidemiological survey on Theileria orientalis was conducted in a cattle population of Fujian province in China. The screening polymerase chain reaction assay showed that 23 (45%) of 51 blood samples were positive for T. orientalis. DNA sequencing of the major piroplasm surface antigen-encoding gene indicated the presence of 3 different genotypes in the study area(types 1，2，and 5) and identified T. orientalis types 1 and 2 as the major genotypes in the sample population.  Because type 2 (Ikeda type in Japan) has been recognized as a relatively virulent genotype of T. orientalis，control and preventive measures to minimize the incidence of infectious diseases among cattle bred in China are necessary.
Palavras-chave: Theileria orientalis; Cattle; China; Epidemiology; Genotype.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3098

Molecular Epidemiological Survey of Theileria orientalis in Thua Thien Hue Province, Vietnam
Provedor de dados: 19
Autores: KHUKHUU, Altangerel; LAN, Dinh Thi Bich; LONG, Phung Thang; UENO, Akio; LI, Yan; LUO, Yuzi; MACEDO, Alan Caine Costa de; MATSUMOTO, Kotaro; INOKUMA, Hisashi; KAWAZU, Shin-Ichiro; IGARASHI, Ikuo; XUAN, Xuenan; YOKOYAMA, Naoaki; 松本, 高太郎; 猪熊, 壽; 河津, 信一郎; 五十嵐, 郁男; 玄, 学南; 横山, 直明.
Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report the findings of a molecular epidemiological survey of T. orientalis in 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, 21 goats and 85 blood-sucking ticks of cattle in the Thua Thien Hue province of Vietnam. The major piroplasm surface protein (MPSP) gene of T. orientalis was detected using polymerase chain reaction from 13 cattle (13.8%), 11 water buffaloes (25.6%), 1 sheep (4.8%) and 9 ticks (10.6%). Phylogenetic analysis using MPSP gene sequences showed the presence of seven genotypes, four previously categorized genotypes (Types 1, 3, 5 and 7) and three new genotypes (Types N-1, N-2 and N-3).
Palavras-chave: Cattle; Genotype; MPSP; Theileria orientalis; Vietnam.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3585

Evaluation of inhibitory effect of methylene blue against Babesia and Theileria parasites
Provedor de dados: 19
Autores: Tuvshintulga, B.; Sivakumar, T.; Salama, A.A.; Yokoyama, N.; Igarashi, I; 五十嵐, 郁男; 横山, 直明.
Methylene blue, the first fully synthetic drug, has been widely used in medical treatments. A few decades ago, this drug was used as an antimalarial agent. In this study, the in vitro inhibitory effect of methylene blue on Babesia bovis, B. bigemina, B. caballi, and Theileria equi (B. equi) and the in vivo inhibitory effect on B. microti were evaluated. Methylene blue significantly inhibited the growth of B. bovis, B. caballi, and T. equi at a 0.1 μM concentration, while B. bigemina was significantly inhibited at 0.01 μM, on day 3 of cultivation. The half maximal inhibitory concentrations (IC50) of methylene blue against B. bovis, B. bigemina, B. caballi, and T. equi were calculated as 0.83±0.02, 0.68±0.09, 0.54±0.14, and 0.49±0.06 μM, respectively. The...
Palavras-chave: Methylene blue; Babesia; Theileria; In vitro; In vivo.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4242

Inhibitory effect of apicidin on in vitro and in vivo growth of Babesia parasites
Provedor de dados: 19
Autores: Munkhjargal, T; Aboulaila, M.R.A; Sivakumar, T; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男.
Apicidin, a histone deacetylase inhibitor, has a broad spectrum of anti-protozoal activities against apicomplexan parasites. In the present study, we evaluated the inhibitory effect of apicidin on the asexual growth of bovine Babesia parasites (B. bovis and B. bigemina) in vitro, as well as on the in vivo growth of B. microti in mice. The growth of B. bovis and B. bigemina was significantly inhibited in the presence of 3 ng/ml apicidin. Complete inhibition of B. bovis and B. bigemina growth was achieved on the fourth and third days, respectively, after treatment with 729 ng/ml apicidin. These parasites failed to grow again even when drug-free medium was substituted. The 50% inhibitory concentrations of apicidin against B. bovis and B. bigemina were...
Palavras-chave: Apicidin; B .bovis; B .bigemina; B. microti; Asexual growth.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2386

20-Methylcholanthrene誘発腫瘍担癌マウスに対するトキソプラズマ溶解抗原の治療的投与効果
Provedor de dados: 19
Autores: 宮原, 和郎; 本多, 字里子; 戸瀬, 信一; 櫻井, 治久; 五十嵐, 郁男; 齋藤, 篤志; 広瀬, 恒夫; 鈴木, 直義; MIYAHARA, Kazuro.
Therapeutic effects of Toxoplasma lysate antigen (TLA) were studied in mice bearing the tumor in the second passage of 20-methylcholanthrene (MC)-induced tumor cells. Intramuscular administration of TLA 7 days after the tumor-cell inoculation caused apparent inhibition of the tumor growth on day 14. The second treatment facilitated the therapeutic effects. Intravenous transfer of spleen cells prepared from TLA-sensitized mice into tumor-bearing mice also represented the growth inhibitory effects. Prominent effects were seen when the transferred cells were prepared 5 days after sensitization of donor animals. The inhibitory effects were absent in the groups transferred only the adherent cells or the non-adherent cells prepared from sensitized mice. The...
Palavras-chave: Methylcholanthrene-induced tumor; Mouse spleen cell therapeutic effect Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4062

モンゴルにおける家畜のピロプラズマ病の疫学に関する調査研究
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
平成11年度~平成12年度科学研究費補助金基盤研究(B)(2)研究成果報告書11691167
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2286

原虫・宿主相互細胞機能の免疫病理学的研究
Provedor de dados: 19
Autores: 横山, 直明; 五十嵐, 郁男; Yokoyama, Naoaki; Igarashi, Ikuo.
発行年は推定
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3506

Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23
Provedor de dados: 19
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男.
To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the...
Palavras-chave: Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/129

トキソブラズマ溶解抗原(TLA)によって誘導される細胞障害性における脾臓の付着性細胞の役割
Provedor de dados: 19
Autores: MIYAHARA, Kazuro; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男.
In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with...
Palavras-chave: Adherent cell; Cytotoxic activity; Macrophage; Spleen cell; Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4065

拠点総括
Provedor de dados: 19
Autores: 五十嵐, 郁男.
発行年は推定
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3503

トキソプラズマ溶解抗原(TLA)で誘導されるマウス脾臓内の細胞障害性細胞の性質
Provedor de dados: 19
Autores: MIYAHARA, Kazuro; MATSUMOTO, Toru; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男.
Spleen cells from Toxoplasma lysate antigen (TLA)-sensitized BALB/c mice showed the strong cytotoxic activity against both natural killer (NK)-sensitive cells (YAC-1 and RL♂-1) and NK-insensitive cells (P-815), when incubated with TLA or recombinant human IL-2 (rhIL-2). The increment of TLA concentration in culture medium increased the cytotoxic activity. Treatment of effector cells; spleen cells from TLA-sensitized mice incubated with TLA, with anti-asialo GM1 or anti-Thy-1 plus complement inhibited the cytotoxic activity of effector cells, whereas treatment with anti-mouse Lyt-2.2 serum plus complement had no effect on the cytotoxic activity. Treatment of spleen cells from TLA-sensitized mice with anti-asialo GM1 and/or anti-Thy-1 plus complement...
Palavras-chave: Cytotoxic activity; Mouse; Phenotype; Spleen cell; Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4063

First detection of Babesia venatorum (EU1) in Ixodes persulcatus ticks in Mongolia
Provedor de dados: 19
Autores: Tuvshintulga, B.; Battsetseg, B.; Battur, B.; Myagmarsuren, P.; Narantsatsral, S.; Sivakumar, T.; Takemae, H.; Igarashi, I.; Inoue, N.; Yokoyama, N.; 五十嵐, 郁男; 井上, 昇; 横山, 直明.
Babesia venatorum, formerly known as Babesia sp. EU1, is a zoonotic hemoprotozoan parasites that commonly infects deer. In the present study, we investigated B. venatorum infection in Ixodes persulcatus, an important tick vector capable of transmitting several tick-borne pathogens that cause babesiosis, encephalitis, tularemia, and Lyme diseases. DNA samples extracted from questing I. persulcatus ticks (n=63) that had been collected in Selenge province of Mongolia in 2012 and 2013 were screened for B. venatorum using a nested PCR assay. The findings showed that two of 63 DNA samples were positive for B. venatorum. The 18S rRNA sequences amplified from B. venatorum-positive DNA samples shared high identity scores (96.1–99.9%) with known B. venatorum...
Palavras-chave: Babesia venatorum; 18S rRNA; Ixodes persulcatus; Mongolia.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4243

FUCOIDAN INHIBITS THE IN VITRO GROWTH OF BABESIA BOVIS
Provedor de dados: 19
Autores: Takabatake, Noriyuki; Hashiba, Shintaro; Bork, Sabine; Okamura, Masashi; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Palavras-chave: Babesia bovis; Fucoidan.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/646

CURRENT SERODIAGNOSTIC MEASURES FOR EQUINE BABESIOSIS
Provedor de dados: 19
Autores: Hirata, Haruyuki; Yokoyama, Naoaki; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; Babesia caballi; Diagnosis; ELISA; ICT.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/638

Identification and Characterization of a Trypanosoma congolense 46 kDa Protein as a Candidate Serodiagnostic Antigen
Provedor de dados: 19
Autores: ZHOU, Mo; SUGANUMA, Keisuke; RUTTAYAPORN, Ngasaman; NGUYEN, Thu-Thuy; YAMASAKI, Shino; IGARASHI, Ikuo; KAWAZU, Shin-ichiro; SUZUKI, Yasuhiko; INOUE, Noboru; 五十嵐, 郁男; 井上, 昇.
Trypanosoma congolense is a major livestock pathogen in Africa, causing large economic losses with serious effects on animal health. Reliable serodiagnostic tests are therefore urgently needed to control T. congolense infection. In this study, we have identified one T. congolense protein as a new candidate serodiagnostic antigen. The 46.4 kDa protein (TcP46, Gene ID: TcIL3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. The complete nucleotide sequences of TcP46 contained an open reading frame of 1,218 bp. Southern blot analysis indicated that at least two copies of the TcP46 gene were tandemly-arranged in the T. congolense genome. The recombinant TcP46 (rTcP46) was expressed in Escherichia coli as a glutathione...
Palavras-chave: Diagnosis; ELISA; Nagana; TcP46; Trypanosoma.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3983

Expression Patterns of the Implantation-associated Genes in the Uterus during the Estrous Cycle in Mice
Provedor de dados: 19
Autores: LEE, Dong-Soo; YANAGIMOTO UETA, Yoshiko; XUAN, Xuenan; IGARASHI, Ikuo; FUJISAKI, Kozo; SUGIMOTO, Chihiro; TOYODA, Yutaka; SUZUKI, Hiroshi; 玄, 学南; 五十嵐, 郁男; 鈴木, 宏志.
The mRNA expression patterns of EGF, HB-EGF, Amphiregulin, EGF receptor, IGF-1, CSF-1, IL-1 alpha, IL-1 beta, IL-1 receptor type 1, IL-1 receptor antagonist, LIF, COX-1, COX-2, Mucin-1, calcitonin, and rat USAG-1 mouse homologue, all of which are involved in the process of conceptus implantation to the endometrium, were examined during the estrous cycle by means of real-time quantitative PCR. COX-2, HB-EGF, LIF, Mucin-1, CSF-1, IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist were temporally regulated during the estrous cycle and highly expressed during the estrous stage. In the case of COX-1, EGF, IGF-1, and EGF receptor, the highest mRNA expression was during the diestrous stage. In contrast, the rat USAG-1 mouse homologue mRNA expression did not...
Palavras-chave: Estrous cycle; Implantation; Mice; Ovarian hormone.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3022

日本におけるOIEリファレンスセンター(II) : 帯広畜産大学原虫病研究センターにおけるOIEリファレンスセンター認定施設としての取り組み
Provedor de dados: 19
Autores: 五十嵐, 郁男; IGARASHI,ikuo.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3853

Involvement of a Host Erythrocyte Sialic Acid Content in Babesia bovis Infection
Provedor de dados: 19
Autores: TAKABATAKE, Noriyuki; OKAMURA, Masashi; YOKOYAMA, Naoaki; OKUBO, Kazuhiro; IKEHARA, Yuzuru; IGARASHI, Ikuo; 横山, 直明; 五十嵐, 郁男.
Host sialic acid (SA) has recently been suggested to play an important role in erythrocyte (RBC) infection by Babesia spp. The present study attempted to further determine the specific type of SAs important in the RBC invasion. Bovine RBC was found to bear abundant α2-3-linked SA residues but not α2-6-linked SA in nature, confirmed by flow cytometric analysis of the neuraminidase (Nm)-treated RBCs. Lectin-blot analyses revealed the removal of α2-3-linked SAs from the 97-, 33-, and 31-kDa bands by the Nm treatment. Addition of the Nm-treated RBCs into an in vitro culture of B. bovis resulted in a decreased population of the parasitized RBCs. The thin smear samples from the cultures were then observed under a confocal laser scanning microscope after staining...
Palavras-chave: Babesia bovis; Erythrocyte; Invasion; Sialic acid.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4166

原虫病研究センター国際シンポジウムにおける特別講演，バベシア病の診断法に関する研究打合せ，獣医学教育における国際協力についての打合せ
Provedor de dados: 19
Autores: 五十嵐, 郁男.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/614

The Role of Scavenger Receptor Type A (SR-A) during Infection with Babesia microti
Provedor de dados: 19
Autores: Kamada, Takenori; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Suzuki, Hiroshi; Kodama, Tatsuhiko; Suzuki, Naoyoshi; Toyoda, Yutaka; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行; 鈴木, 宏志.
Palavras-chave: Babesiosis; Protective immune response; Knock-out mouse; SRKO.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/265

Serodiagnosis of Toxoplasma gondii in ducks from Behera Governorate, Egypt
Provedor de dados: 19
Autores: AbouLaila, M; El-Bahy, N; Hilali, M; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男.
Toxoplasma gondii is an important zoonotic parasite. The diagnosis of infection in animals is an important tool to prevent human infection. In the present study, Toxoplasma gondii was diagnosed by using the modified agglutination test (MAT) in ducks from Behera Governorate, Egypt. The prevalence of Toxoplasma gondii was 13.9%. The prevalence of Toxoplasma gondii was the highest in the Native breed (17.65%) and in 6-8-months age group (19.4%). End-point titer of 1:25, 1:50, and 1:100 were recorded in 61.9%, 19.05%, and 19.05% of the positive samples, respectively. Duck meat is one source of the Toxoplasma gondii infection for human in Egypt.
Palavras-chave: Toxoplasma gondii; Modified agglutination test (MAT); Ducks; Egypt.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3128

動物性蛋白質資源の生産向上研究グループ総括
Provedor de dados: 19
Autores: 五十嵐, 郁男.
発行年は推定
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3504

Changes in immune serum of toxoplasma gondii following administration of T. gondii lysate antigen
Provedor de dados: 19
Autores: Yoda, Tomoko; Sakurai, Haruhisa; Igarashi, Ikuo; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; 依田, 知子; 櫻井, 治久; 五十嵐, 郁男; 小俣, 吉孝; 齊藤, 篤志; 鈴木, 直義.
Palavras-chave: Toxoplasma; Serum components; Bovine; Toxoplasma lysate antigen; Lymphokines.
Ano: 1990
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1569

ウマバベシア病に対する国際標準診断法の開発
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
2001年度～2004年度科学研究費補助金基盤研究(A)(2)研究成果報告書13356007
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2287

実験的赤血球内寄生原虫症の感染防御に関する基礎的研究
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
平成5年度科学研究費補助金（一般研究（C））研究成果報告書030660318
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2284

ウシ及びウマ・バベシア原虫 in vitro 長期培養法の確立とその応用に関する研究
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2283

Babesia bovis: Subcellular localization of host erythrocyte membrane components during their asexual growth
Provedor de dados: 19
Autores: Okamura, Masashi; Yokoyama, Naoaki; Takabatake, Noriyuki; Okubo, Kazuhiro; Ikehara, Yuzuru; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
In the present study, the subcellular localization of the host red blood cell (RBC) membrane components, the alpha 2-3-linked sialic acid (SA) residues and the lipid bilayer, was observed during the asexual growth of Babesia bovis using two erythrocyte probes, the SA-specific lectin (MALII) and the lipophilic fluorescent (PKH2) probes, respectively. In confocal laser scanning microscopy with MALII, the SA residues on the surface of parasitized RBCs appeared to accumulate into the intracellular parasites as the parasites matured as well as to remain on the surface of extracellular parasites. Furthermore, when PKH2-labeled RBCs were infected with B. bovis, PKH2 signals were also observed around both the intracellular and the extracellular parasites,...
Palavras-chave: Babesia bovis (B. bouis); SA; MALII; RBC; RT; PBS; Babesia equi (B. equi); PV.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1040

Effect of Oligomannose-Coated Liposome-Based Vaccine on Rodent Babesiosis
Provedor de dados: 19
Autores: Ishigame, T; Takabatake, N; Iseki, H; Ota, N; Koyama, A; Igarashi, I; Nishikawa, Y; Ikehara, Y; Kojima, N; Yokoyama, N; 五十嵐, 郁男; 西川, 義文; 横山, 直明.
Oligomannose-coated liposome (OML)-based vaccines have been reported to induce Th1-based immunity against entrapped antigens in immunized animals and to show protective effects against several protozoal diseases. In the present study, we produced a recombinant Babesia rodhaini ribosomal phosphoprotein P0 (rBrP0) that showed immunological cross reactivity with another rodent Babesia parasite, B. microti. We evaluated the efficacy of vaccination with OML-entrapped rBrP0 on B. rodhaini and B. microti infections in mice. Prior immunization with the OML-based rBrP0 vaccine, or with a Freund’s adjuvant-based rBrP0 vaccine, failed to demonstrate any protective effect against lethal infection with B. rodhaini, but the OML-based vaccine did induce protective...
Palavras-chave: Oligomannose-coated liposomes (OML); Vaccine; Babesiosis; Babesia rodhaini; Babesia microti; P0.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2383

A PCR Based Survey of Babesia ovata in Cattle from Various Asian, African and South American Countries
Provedor de dados: 19
Autores: SIVAKUMAR, Thillaiampalam; ASADA, Masahito; BATTSETSEG, Badgar; HUANG, Xiaohong; LAN, Dinh Thi Bich; INPANKAEW, Tawin; P. YBAÑEZ, Adrian; ALHASSAN, Andy; THEKISOE, Oriel M. M.; DE MACEDO, Alan Caine Costa; INOKUMA, Hisashi; IGARASHI, Ikuo; YOKOYAMA, Naoaki; 猪熊, 壽; 五十嵐, 郁男; 横山, 直明.
Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B....
Palavras-chave: AMA-1; Babesia ovata; Cattle; Mongolia; Thailand.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4016

アジア・アフリカにおけるバベシア原虫の分子疫学的調査研究
Provedor de dados: 19
Autores: 五十嵐, 郁男; IGARASHI, IKUO.
2007年度～2010年度科学研究費補助金基盤研究（B）研究成果報告書19405044
Palavras-chave: バベシア病; アジア; アフリカ; 診断; 疫学調査; 遺伝子解析.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3000

Methylcholanthrene自家誘発腫瘍担癌ラットに対するトキソプラズマ溶解抗原の抗腫瘍効果
Provedor de dados: 19
Autores: MIYAHARA, Kazuro; YOKOO, Naoya; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAKATA, Yu; YOSHIDA, Yutaka; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男.
Growth of the tumor autoinduced by 20-methylcholanthrene (MC) in rats was inhibited after administration of Toxoplasma lysate antigen (TLA). The antitumor activity of TLA was most obvious in the early stage of tumoral growth. When TLA was administered to rats before the appearance of tumor, tumor formation was delayed slightly. Histopathological studies revealed dense growths of spindle tumor cells in untreated control rat, while enlarged central necrosis with the infiltration of lymphocytes and neutrophils was apparent in TLA-treated rats. According to the immunohistological examination of tumor tissue with anti-Thy-1 antibody, the rats treated with TLA showed large Thy-1 positive granular cells, whereas the untreated rats indicated only a few small Thy-1...
Palavras-chave: Antitumor activity; Methylcholanthrene-autoinduced tumor; Rat; Thy-1 positive granular cell; Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4064

Genotypic Diversity of Theileria orientalis Detected from Cattle Grazing in Kumamoto and Okinawa Prefectures of Japan
Provedor de dados: 19
Autores: YOKOYAMA, Naoaki; UENO, Akio; MIZUNO, Daisuke; KUBOKI, Noritaka; KHUKHUU, Altangerel; IGARASHI, Ikuo; MIYAHARA, Tokuji; SHIRAISHI, Takashi; KUDO, Ryuta; OSHIRO, Mamoru; ZAKIMI, Satoshi; SUGIMOTO, Chihiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; 横山, 直明; 五十嵐, 郁男; 松本, 高太郎; 猪熊, 壽.
Theileria orientalis is a benign protozoan species that is widely distributed in Japan, yet sometimes causes serious economic losses in the livestock industry. In this study, we conducted a molecular survey based on genes encoding the major piroplasm surface protein (MPSP) and p23 for T. orientalis detected in cattle grazing in southern areas of Japan, consisting of 2 farms in Kumamoto prefecture (Aso and Kuma districts) and 3 farms in Okinawa prefecture (Ishigaki, Iriomote, and Yonaguni Islands). High prevalence rates of T. orientalis infection were shown in all the cattle populations using the diagnostic MPSP- and p23-PCR assays. Phylogenetic analyses revealed 4 MPSP genotypes and 3 p23 genotypes. Furthermore, MPSP genotype-specific PCR methods were...
Palavras-chave: Cattle; Genotype-specific PCR; Japan; MPSP gene; Theileria orientalis.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3584

Detection of Antibodies to Babesia equi by The Latex Agglutination Test with Recombinant Merozoite Antigen-2
Provedor de dados: 19
Autores: Tanaka, Tetsuya; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; EMA-2; Baculovirus; Latex agglutination test.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/329

Purification of Merozoites from In Vitro Cultured Babesia caballi- Infected Equine Erythrocytes
Provedor de dados: 19
Autores: Ikadai, Hiromi; Sato, Masumi; Avarzed, Abgaandorjiin; Nagasawa, Hideyuki; Igarashi, Ikuo; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; 長澤, 秀行; 五十嵐, 郁男.
Palavras-chave: Purification; Merozoite; Babesia caballi.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/272

In vitro growth inhibitory effect of (-)-Epigallocatechin-3-gallate from green tea on the growth of equine Babesia parasites
Provedor de dados: 19
Autores: AbouLaila, M; Terkawi, M.A.; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男.
Palavras-chave: (-)-Epigallocatechin-3-gallate; In vitro; Babesia caballi; Babesia equi.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3124

IN VITRO CULTURE OF THEILERIA ORIENTALIS SERGENTI, AND THE USE OF THE BOVINE-RED BLOOD CELL-SCID MOUSE MODEL IN-AID OF CULTIVATION
Provedor de dados: 19
Autores: Ogata, Takashi; Tsukamoto, Daisuke; Sato, Masumi; Kamio, Tsugihiko; Terada, Yutaka; Igarashi, Ikuo; Claveria, Florencia G.; Fujisaki, Kozo; 五十嵐, 郁男.
Palavras-chave: Adult bovine serum; Bo-RBC SCID mice; In vitro; Theileria orientalis sergenti.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/628

Inhibitory Effects of Pepstatin A and Mefloquine on the Growth of Babesia Parasites
Provedor de dados: 19
Autores: Munkhjargal, Tserendorj; AbouLaila, Mahmoud; Mohamad Alaa Terkawi; Sivakumar, Thillaiampalam; Ichikawa, Madoka; Davaasuren, Batdorj; Nyamjargal, Tserendorj; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
We evaluated the inhibitory effects of pepstatin A and mefloquine on the in vitro and in vivo growths of Babesia parasites. The in vitro growth of Babesia bovis, B. bigemina, B. caballi, and B. equi was significantly inhibited (P &lt; 0.05) by micromolar concentrations of pepstatin A (50% inhibitory concentrations = 38.5, 36.5, 17.6, and 18.1 μM, respectively) and mefloquine (50% inhibitory concentrations = 59.7, 56.7, 20.7, and 4 μM, respectively). Furthermore, both reagents either alone at a concentration of 5 mg/kg or in combinations (2.5/2.5 and 5/5 mg/kg) for 10 days significantly inhibited the in vivo growth of B. microti in mice. Mefloquine treatment was highly effective and the combination treatments were less effective than other treatments....
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3829

Detection of Collagen-cross Reactive Antigenic Components in Toxoplasma gondii
Provedor de dados: 19
Autores: Omata, Yoshitaka; Inoue, Noboru; Yonematsu, Kunio; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Collagen; Cross-reactive antigens; Toxoplasma gondii.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/223