Consulta: creatorFacets:"Igarashi, Ikuo"
Registros recuperados: 64
Data/hora: 11/06/2024 06:27:47

Comparison of Damage to Kidneys and Liver Caused by Lethal Babesia rodhaini Infection and Non-lethal Babesia microti Infection in Mice
Provedor de dados: 19
Autores: Igarashi, Ikuo; Hosomi, Takushi; Kaidoh, Toshiyuki; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Aikawa, Masamichi.
Palavras-chave: Babesia rodhaini; Babesia microti; Kidneys; Liver; Mice infection.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/198

Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam
Provedor de dados: 19
Autores: Liyanagunawardena, Nilukshi; Sivakumar, Thillaiampalam; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Battsetseg, Badgar; Dinh Thi Bich Lan; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia bovis is the most virulent Babesia organism, resulting in a high mortality rate in cattle. The genetic diversity of B. bovis merozoite surface antigens (MSAs), such as MSA-1, MSA-2b, and MSA-2c, might be linked to altered immune profiles in the host animals. The present study aimed to develop type-specific PCR assays for Asian msa-1 genotypes, thereby re-analyzing the genetic diversity of msa-1 in Sri Lanka, Mongolia, and Vietnam. Specific primers were designed for nine Asian msa-1 genotypes, which had been detected based on the phylogeny constructed using msa-1 gene sequences retrieved from the GenBank database. Specificity of the type-specific PCR assays was confirmed using plasmids containing the inserts of msa-1 gene fragments that represent...
Palavras-chave: Asia; Babesia bovis; Genetic diversity; Msa-1; Type-specific PCR.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4489

BABESIA CABALLI : ANALYSIS OF CHROMOSOMES SEPARATED BY PULSE FIELD GEL ELECTROPHORESIS
Provedor de dados: 19
Autores: Ikadai, Hiromi; Kudo, Noboru; Oyamada, Takashi; Igarashi, Ikuo; 五十嵐, 郁男.
Palavras-chave: Babesia caballi; Pulsed field gel electrophoresis; Chromosome; Rhoptry gene.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/642

Preliminary evaluation of oligomannose-coated liposome vaccines against lethal protozoan infections in mice
Provedor de dados: 19
Autores: Kuboki, Noritaka; Tiwananthagorn, Weerawan; Takagi, Hideaki; Nakayama, Tomoko; Xuan, Xuenan; Inoue, Noboru; Igarashi, Ikuo; Tsujimura, Kunio; Ikehara, Yuzuru; Kojima, Naoya; Yokoyama, Naoaki.
The oligomannose-coated liposome (OML) vaccine is known to induce cellular immunity specific for the encapsulated antigen in immunized mice. In the present study, we preliminarily evaluated the effect of the OML vaccine encapsulating the soluble protozoan lysate of Toxoplasma gondii, Trypanosoma brucei gambiense, or Babesia rodhaini on the corresponding protozoan infections in mice. After the challenge of T. gondii, the OML vaccine group avoided the high mortality resulting from acute infection that was dominantly observed in other control groups. During the infectious course, the development of the T. gondii-specific antibody, which is an indicator of humoral immunity, was constantly controlled at a lower level in the surviving mice of the OML vaccine...
Palavras-chave: Oligomannose-coated liposome (OML); OML; Soluble protozoan antigen; Antibody responses.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1050

Restorative Effects of A Newly Synthesized Peptide, Obiopeptide-1, in Cyclophosphamide- or Carrageenan-pretreated Mice Infected with Opportunistic Bacteria
Provedor de dados: 19
Autores: Fujii, Yasuhiro; Maki, Yoshiyuki; Ito, Masanari; Saito, Atsushi; Igarashi, Ikuo; Ono, Kenichiro; Itoh, Kikuji; Suzuki, Naoyoshi.
Large numbers of cyclophosphamide- or carrageenan-pretreated immunosuppressed adult ddY mice died within 10 days after inoculation with several species of opportunistic bacteria, including P. aeruginosa, K. pneumoniae, E. coli, S. aureus and methicillin resistant S. aureus. When immunosuppressed mice were administered bacteria in combination with two 100 μg/mouse intramuscular doses of a newly synthesized peptide, Obiopeptide-1 (OP-1), survival rates increased significantly. At 24 and 48 hours after intraperitoneal inoculation with P. aeruginosa, counts of viable organisms from the livers, spleens, lungs, hearts, and kidneys of mice that were administered cyclophosphamide in combination with OP-1 were significantly lower than counts from mice that were...
Palavras-chave: Immunoregulator; Synthesized peptide; Immunosuppression; Cyclophosphamide; Carrageenan; Ooiopeptide.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/166

バベシア原虫感染防御抗原の発生工学的解析
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
1997年度～2000年度科学研究費補助金基盤研究(B)(2)研究成果報告書09460143
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2285

Cellualr Subsets Involved in Protective Immunity to Babesiarodhaini Infection in BALB/c Mice
Provedor de dados: 19
Autores: Shimada, Terumasa; Igarashi, Ikuo; Maki, Yoshiyuki; Claveria, Florencia G.; Saito, Atsushi; Suzuki, Naoyoshi.
Protective immune responses and the functional role of spleen cells in mice infected with Babesia rodhaini were examined with an in vitro proliferation assay systems and by in vivo passive transfer of spleen cells to uninfected mice. Mice that resolved primary babesial infection after chemotherapy (Babesia immune mice) had transient and low parasitemia after challenge infection and high rates (75%) of survival. Babesia hyperimmune mice, by contrast, had no detectable parasitemia after challenge and 100% survival. Proliferative response of spleen cells to Babesia lysate antigen (BLA) were determined for mice from both groups. This proliferative response was inhibited by treatment of spleen cells with anti-T cell serum and monoclonal antibody (MAb) to Lyt l...
Palavras-chave: Babesia rodhaini; Cell transfer; Spleen cells; Protective immunity.
Ano: 1991
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/157

Toxoplasma Lysate Antigen (TLA144)-Ig8 Antigenic Component : Binding and Induction of Cytotoxic Cell Activity in Mouse Spleen Cells
Provedor de dados: 19
Autores: Kato, Mihoko; Maki, Yoshiyuki; Inoue, Noboru; Omata, Yoshitaka; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Cytotoxic cell activity; NK cell; LAK cell; Toxoplasma lysate antigen.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/216

TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS
Provedor de dados: 19
Autores: Fujii, Kei; Yokoyama, Naoaki; Takabatake, Noriyuki; Suzuki, Hiroshi; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 鈴木, 宏志; 玄, 学南; 五十嵐, 郁男.
In hemoprotozoa，gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study，a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection，clear positive fluorescences were detected in some parasite bodies， indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However， the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that...
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/631

Molecular and Immunological Characterization of a Novel 32-kDa Secreted Protein of Babesia microti
Provedor de dados: 19
Autores: Ooka, Hideo; Terkawi, Mohamad A; Cao, Shinuo; Aboge, Gabriel; Goo, Youn-Kyoung; Luo, Yuzi; Li, Yan; Nishikawa, Yoshifumi; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
A cDNA encoding the Babesia microti 32-kDa protein was identified by serological immunoscreening of a cDNA expression library and designated as BmP32. The full length of BmP32 contains an open reading frame of 918 base pairs consisting of 306 amino acids having a significant homology with B. microti secreted antigen 1. Antiserum raised against recombinant protein (rBmP32) specifically reacted with a 32-kDa native protein of the parasite lysate using western blot analysis. The indirect immunofluorescent antibody test showed a preferable localization of BmP32 in the cytoplasm of the intra- and extracellular parasites. Moreover, BmP32 was secreted in the cytosol of infected erythrocytes, especially during the peak parasitemia and the recovery phase of the...
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3820

Genetic diversity and antigenicity variation of Babesia bovis merozoite surface antigen-1 (MSA-1) in Thailand
Provedor de dados: 19
Autores: Tattiyapong, Muncharee; Sivakumar, Thillaiampalam; Takemae, Hitoshi; Simking, Pacharathon; Jittapalapong, Sathaporn; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia bovis, an intraerythrocytic protozoan parasite, causes severe clinical disease in cattle worldwide. The genetic diversity of parasite antigens often results in different immune profiles in infected animals, hindering efforts to develop immune control methodologies against the B. bovis infection. In this study, we analyzed the genetic diversity of the merozoite surface antigen-1 (msa-1) gene using 162 B. bovis-positive blood DNA samples sourced from cattle populations reared in different geographical regions of Thailand. The identity scores shared among 93 msa-1 gene sequences isolated by PCR amplification were 43.5-100%, and the similarity values among the translated amino acid sequences were 42.8-100%. Of 23 total clades detected in our...
Palavras-chave: Antigenicity variation; Babesia bovis; Msa-1; Genetic diversity; Thailand.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4490

IN VITRO GROWTH INHIBITORY EFFECTS OF COMBINED APPLICATIONS OF CLOTRIMAZOLE, KETOCONAZOLE, AND CLODINAFOP-PROPARGYL ON EQUINE BABESIA PARASITES
Provedor de dados: 19
Autores: Bork, Sabine; Yokoyama, Naoaki; Okamura, Masashi; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Recently，we reported the in vitro growth inhibitory effects of the imidazole derivatives，clotrimazole(CLT) and ketoconazole (KC)，and the herbicide，clodinafop-propargyl(CP)，against bovine and equine Babesia parasites. Additionally，in the bovine species，several combined applications of the CLT，KC，and CP were reported to significantly increase the inhibitory effects. In this study，similar combination tests were carried out in order to confirm possible synergistic effects in the equine species，Babesia caballi and Babesia equi. Combinations of CLT/KC， CLT/CP，and CLT/KC/CP exerted a significantly enhanced growth inhibitory efficacy in B. caballi，in contrast to B. equi，where no synergistic effects were observed in any of the combinations. Our results suggest that...
Palavras-chave: In vitro; Babesia; Clotrimazole; Ketoconazole; Clodinafop-propargyl.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/637

Anil-Babesia rodhaini Monoclonal Antibodies: Effect against Babesia rodhaini and Cross-reactivity with Human Starin of Babesia microti
Provedor de dados: 19
Autores: Claveria, Florencia G.; Omata, Yoshitaka; Takehara, Tokuma; Saito, Atsushi; Maki, Yoshiyuki; Igarashi, Ikuo; Suzuki, Naoyoshi.
Anti-Babesia rodhaini monoclonal antibodies (mAb), namely: 1-E7, 2-H2 and 3-B8, significantly suppressed the development of high parasitemia in BALB/c mice infected with B. rodhaini and all mAb-treated mice survived the infection. While, only monoclonal antibody 3-B8 showed some inhibitory effect against Babesia microti, with mice showing high parasitemia of 18.04 ± 2.69 %, at 9 days post-exposure. Westernblot analysis of B. rodhaini and B. microti parasite extract reacted with anti-B. rodhaini monoclonal antibodies showed cross-reactive bands of molecular weights 62 and 55 kilodaltons. Comparison of the antigenic components of B. rodhaini and B. microti using polyspecific sera revealed several shared or common parasite antigens of molecular weights 62,...
Palavras-chave: Monoclonal antibodies; Cross-reactivity; Babesia rodhaini; Babesia microti.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/171

Different Activation of Lymphocytes in Reaction to Babesia microti Infection in iNOS-/- and Wildtype Mice
Provedor de dados: 19
Autores: Remer, Katharina; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行.
Palavras-chave: Lymphocytes; Babesia microti; Spleen; INOS-/- mice; CD4+ cells; CD8+ cells; IFN-γ; Antibody-titer.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/263

Cellular localization of Babesia bovis rhoptry-associated protein 1 in the merozoite stage with immuno-electron microscopy
Provedor de dados: 19
Autores: Matsuo, Tomohide; Yokoyama, Naoaki; Suthisak, Boonchit; Fujisaki, Kozo; Igarashi, Ikuo.
We examined the detailed cellular localization of the rhoptry-associated protein-1 (RAP-1) of Babesia bovis merozoites by the post-embedding method of immuno-electron microscopy (IEM) using the anti-RAP-1 monoclonal antibody (mAb) 1C1 in order to substantiate the result in our previous report by an indirect immunofluorescent antibody test and the pre-embedding method of IEM. RAP-1 slightly distributed only in cytoplasm of merozoites, especially around the nucleus, but not in the rhoptry organelle at an early stage after invasion into a red blood cell. Then, the RAP-1 was accumulated in the rhoptry organelle and was also found in the iRBC cytoplasm, which suggests that synthesis and release of RAP-1 may occur in the iRBC. Finally, the RAP-1 was found within...
Palavras-chave: Babesia bovis RAP-1 invasion; Escape RBC.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/145

Characterization of P15 Antigen of Cryptosporidium parvum Expressed by a Recombinant Vaccinia Virus
Provedor de dados: 19
Autores: Xuan, Xuenan; Zhang, Sofa; Kamio, Tsugihiko; Tsushima, Y.; Kamada, Takenori; Nishikawa, Yoshifumi; Otsuka, Haruki; Karanis, Panagiotis; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; 玄, 学南; 西川, 義文; 五十嵐, 郁男.
Palavras-chave: C. parvum; P15; Vaccinia virus; Subunit vaccine.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/314

Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs.
Provedor de dados: 19
Autores: Fukumoto, Shinya; Tamaki, Yoh; Okamura, Masashi; Bannai, Hiroshi; Yokoyama, Natsuko; Suzuki, Tomoko; Igarashi, Ikuo; Suzuki, Hiroshi; Xuan, Xuenan.
A heterologous prime-boost immunization regime with priming DNA followed by recombinant vaccinia virus expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In this study, we constructed a recombinant plasmid and vaccinia virus, both of which expressed P50 of Babesia gibsoni, to investigate the immunogenicity and protective efficacy of a heterologous prime-boost immunization against canine babesiosis. The dogs immunized with the prime-boost regime developed a significantly high level of specific antibody against P50 when compared with the control groups, and the antibody level was strongly increased after a booster immunization with a recombinant vaccinia virus. The prime-boost immunization...
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/812

Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction
Provedor de dados: 19
Autores: Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi,  and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction（PCR）method．The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001％. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281

Production of Transgenic Mice Carrying p30 Gene Encoding Major Surface Antigen of Toxoplasma gondii
Provedor de dados: 19
Autores: Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行.
Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254

Molecular Prevalence of Different Genotypes of Theileria orientalis Detected from Cattle and Water Buffaloes In Thailand
Provedor de dados: 19
Autores: Altangerel, Khukhuu; Sivakumar, Thillaiampalam; Inpankaew, Tawin; Jittapalapong, Sathaporn; Terkawi, Mohamad Alaa; Ueno, Akio; Xuan, Xuenan; Igarashi, Ikuo; Yokoyama, Naoaki; 玄, 学南; 五十嵐, 郁男; 横山, 直明.
Here we report on an epidemiological study regarding the molecular prevalence of different genotypes of Theileria orientalis present among domestic cattle and water buffalo populations bred in Thailand. A phylogenetic analysis based on the parasitic gene encoding a major piroplasm surface protein revealed the presence of 5 genotypes (Types 1, 3, 5, 7, and N-3) in cattle and 7 genotypes (Types 1, 3, 4, 5, 7, N-2, and N-3) in water buffaloes. Types 4, 7, and N-3 of T. orientalis were reported for the first time in water buffaloes. The previously reported C and Thai types from Thailand clustered as types 7 and 6, respectively, in the present analysis. Great similarities were observed among nucleotide sequences of isolates of the same genotype from cattle and...
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3592

RBC invasion and invasion-inhibition assays using free merozoites isolated after cold treatment of Babesia bovis in vitro culture
Provedor de dados: 19
Autores: Ishizaki, Takahiro; Sivakumar, Thillaiampalam; Hayashida, Kyoko; Tuvshintulga, Bumduuren; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia bovis is an apicomplexan hemoprotozoan that can invade bovine red blood cells (RBCs), where it multiplies asexually. RBC invasion assays using free viable merozoites are now routinely used to understand the invasion mechanism of B. bovis, and to evaluate the efficacy of chemicals and antibodies that potentially inhibit RBC invasion by the parasite. The application of high-voltage pulses (high-voltage electroporation), a commonly used method to isolate free merozoites from infected RBCs, reduces the viability of the merozoites. Recently, a cold treatment of B. bovis in vitro culture was found to induce an effective release of merozoites from the infected RBCs. In the present study, we incubated in vitro cultures of B. bovis in an ice bath to...
Palavras-chave: Babesia bovis; Cold treatment; Invasion-inhibition assay; Merozoite isolation; RBC invasion.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4488

Preliminary Survey on Horse Serum Indirect Fluorescence Antibody Titers in Japan against Babesia equi and Babesia caballi (Onderstepoort strain) Antigen
Provedor de dados: 19
Autores: Suzuki, Naoyoshi; Igarashi, Ikuo; Abgaandorjiin, A.; Waal, D. T. De; Nagasawa, Hideyuki; Omata, Yoshitaka; Saito, Atsushi; Oyamada, Takashi.
Palavras-chave: Babesia caballi; Babesia equi; Sera diagnosis; IFAT; Survey.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/243

Role of CD4⁺ T Cells in the Control of Primary Infection with Babesia microti in Mice
Provedor de dados: 19
Autores: Igarashi, Ikuo; Waki, Seiji; Ito, Mamoru; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; 五十嵐, 郁男.
Palavras-chave: Babesia microti; Nude mice; CD4⁺ T Cells; Primary infection; Gamma interferon.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/218

In vitro inhibitory effect of fosmidomycin on the asexual growth of Babesia bovis and Babesia bigemina
Provedor de dados: 19
Autores: Sivakumar, T; Aboulaila, M. R. A.; Khukhuu, A; Iseki, H; Alhassan, A; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
The in vitro inhibitory effect of fosmidomycin was evaluated against the asexual growth of bovine Babesia parasites (Babesia bovis and Babesia bigemina). In the cultures with these parasites, severe reduction of parasite growth was observed by the addition of 1.25 μg/ml of the final drug concentration. Under these conditions, B. bigemina was completely cleared until the third day of cultivation. On the other hand, B. bovis was completely eliminated on the fourth day when the concentration was 6.25 μg/ml in the culture. These parasites failed to grow again when a drug-free medium was substituted for the subsequent cultivation. The IC50 values of fosmidomycin against B. bovis were determined as 0.88 and 0.63 μg/ml in the in vitro cultures with...
Palavras-chave: Fosmidomycin; Babesia bovis; Babesia bigemina; Asexual growth; In vitro culture.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2235

Construction of Recombinant Feline Herpesvirus Type 1 Expressing Toxoplasma gondii SRS1
Provedor de dados: 19
Autores: Mishima, Masayuki; Xuan, Xuenan; Takeiri, Akira; Makala, Levi H. C; Igarashi, Ikuo; Fujisaki, Kozo; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Toxoplasma; Vaccine; SRS1; FHV.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/343

Survey of Sarcocystis spp. Infection in Slaughfered Pigs in East Hokkaido, Japan
Provedor de dados: 19
Autores: Omata, Yoshitaka; Heydorn, Alfred Otto; Heidrich, Hans-G.; Igarashi, Ikuo; Saito, Atsushi; Toba, Hideo; Suzuki, Naoyoshi.
Palavras-chave: Pig; Sarcocystis; Survey.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/182

Non-invasive Method of Identification of SAG-1 Transgenic Mice by PCR Analysis of Oral Wash Cells
Provedor de dados: 19
Autores: Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南.
Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311

Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle
Provedor de dados: 19
Autores: Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%)....
Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142

A longitudinal study of Babesia and Theileria infections in cattle in Sri Lanka
Provedor de dados: 19
Autores: Sivakumar, Thillaiampalam; Kothalawala, Hemal; Weerasooriya, Gayani; Seekkuge Susil Priyantha Silva; Puvanendiran, Sumathy; Munkhjargal, Tserendorj; Igarashi, Ikuo; Yokoyama, Naoaki.
Throughout the world, infections with the Babesia and Theileria parasites often result in economically significant clinical disease in cattle. We conducted a longitudinal survey of Babesia and Theileria infections in cattle from the Polonnaruwa (n?=?75; dry zone) and Nuwara Eliya (n?=?161; wet zone) districts of Sri Lanka. DNA from blood samples collected in June, September, and December 2014 and March 2015 was screened for Babesia bovis, Babesia bigemina, Theileria annulata and Theileria orientalis using specific polymerase chain reactions (PCRs). Additionally, serum samples collected from the animals were screened using enzyme-linked immunosorbent assays (ELISAs) to detect B. bovis- and B. bigemina-specific antibodies. All of the animals surveyed in...
Palavras-chave: Babesia; Cattle; Longitudinal study; Sri Lanka; Theileria.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4567

CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS
Provedor de dados: 19
Autores: Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624

IMMUNOSCREENING OF BABESIA RODHAINI GENES ENCODING IMMUNOGENIC PROTEINS FROM THE CDNA EXPRESSION PHAGE LIBRARY
Provedor de dados: 19
Autores: Fujii, Kei; Takabatake, Noriyuki; Boonchit, Suthisak; Hirata, Haruyuki; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Palavras-chave: Babesia rodhaini; Babesiosis; Immunoscreening; CDNA expression library.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/626

Seroprevalence of Babesia bovis, B-bigemina, Trypanosoma evansi, and Anaplasma marginale antibodies in cattle in southern Egypt
Provedor de dados: 19
Autores: Fereig, Ragab M.; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; AbouLaila, Mahmoud Rezk; Guswanto, Azirwan; Thu-Thuy Nguyen; Mohamed, Adel Elsayed Ahmed; Inoue, Noboru; Igarashi, Ikuo; Nishikawa, Yoshifumi.
Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginate infections cause serious diseases in cattle, and are primarily transmitted by arthropod vectors (ticks for B. bovis, B. bigemina, and A. marginate and various types of flies for T. evansi). In the last few years, there have been many reports of a high prevalence of certain protozoan infections in northern Egypt, but no accurate or adequate data are available for the southern regions. Therefore, in this study, we screened for evidence of such diseases in economically important cattle species using serum samples. The seroprevalence of protozoan infections in cattle was determined with enzyme-linked immunosorbent assays using species-specific diagnostic antigens. In a total of 301 cattle...
Palavras-chave: Babesia bovis; Babesia bigemina; Trypanosoma evansi; Anaplasma marginale; Cattle; Egypt.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4579

モンゴルにおける家畜のピロプラズマ病の疫学に関する調査研究
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
平成11年度~平成12年度科学研究費補助金基盤研究(B)(2)研究成果報告書11691167
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2286

原虫・宿主相互細胞機能の免疫病理学的研究
Provedor de dados: 19
Autores: 横山, 直明; 五十嵐, 郁男; Yokoyama, Naoaki; Igarashi, Ikuo.
発行年は推定
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3506

Babesia rodhaini and Babesia microti : Cross-Immunity and Cross-Antigens
Provedor de dados: 19
Autores: Inoue, Noboru; Omata, Yoshitaka; Igarashi, Ikuo; Saito, Atsushi; Claveria, Florencia G.; Suzuki, Naoyoshi.
Palavras-chave: Babesia; Rodent; Cross-immunity; Cross-reactive antigens.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/210

Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23
Provedor de dados: 19
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男.
To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the...
Palavras-chave: Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/129

FUCOIDAN INHIBITS THE IN VITRO GROWTH OF BABESIA BOVIS
Provedor de dados: 19
Autores: Takabatake, Noriyuki; Hashiba, Shintaro; Bork, Sabine; Okamura, Masashi; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
Palavras-chave: Babesia bovis; Fucoidan.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/646

CURRENT SERODIAGNOSTIC MEASURES FOR EQUINE BABESIOSIS
Provedor de dados: 19
Autores: Hirata, Haruyuki; Yokoyama, Naoaki; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; Babesia caballi; Diagnosis; ELISA; ICT.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/638

Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
Provedor de dados: 19
Autores: Zhou, Mo; Cao, Shinuo; Luo, Yuzi; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Iguchi, Aiko; Vudriko, Patrick; Terkawi, Mohamad Alaa; Lowenstein, Mario; Kern, Angela; Nishikawa, Yoshifumi; Suzuki, Hiroshi; Igarashi, Ikuo; Xuan, Xuenan.
Background: Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of B. canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection. Methods: A complementary DNA (cDNA) expression library was constructed from the mRNA of B. canis and immunoscreened using B. canis-infected dog sera. The cDNAs encoding a merozoite surface antigen and a secreted antigen protein were identified and designated as BcMSA1 and BcSA1, respectively. The recombinant BcMSA1...
Palavras-chave: Babesia canis; Canine babesiosis; BcMSA1; BcSA1; ELISA; Immunochromatographic tests.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4420

The Role of Scavenger Receptor Type A (SR-A) during Infection with Babesia microti
Provedor de dados: 19
Autores: Kamada, Takenori; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Suzuki, Hiroshi; Kodama, Tatsuhiko; Suzuki, Naoyoshi; Toyoda, Yutaka; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行; 鈴木, 宏志.
Palavras-chave: Babesiosis; Protective immune response; Knock-out mouse; SRKO.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/265

Babesia ovata: Taxonomy, phylogeny and epidemiology
Provedor de dados: 19
Autores: Sivakumar, Thillaiampalam; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia ovata, which is transmitted by Haemaphysalis longicornis, is an intraerythrocytic protozoan parasite of cattle. Based on its morphology, B. ovata is classified as a large-type Babesia. The developmental stages of B. ovata have been described both in cattle and the tick vector. In infected adult female ticks, the parasite is transovarially transmitted to the tick eggs. The sexual reproduction of B. ovata has been demonstrated in the tick midgut. The diagnostic tools that are currently available for the specific detection of B. ovata in cattle include microscopy and polymerase chain reaction assays. The development of improved molecular and serological diagnostic tools has been constrained by the limited availability of genetic data. B. ovata has...
Palavras-chave: Babesia ovata; Cattle; Epidemiology; Phylogeny; Taxonomy.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4555

The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia
Provedor de dados: 19
Autores: Tuvshintulga, Bumduuren; Sivakumar, Thillaiampalam; Battsetseg, Badgar; Narantsatsaral, Sandag-ochir; Enkhtaivan, Batsaikhan; Battur, Banzragch; Hayashida, Kyoko; Okubo, Kazuhiro; Ishizaki, Takahiro; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia microti is a tick-transmitted zoonotic hemoprotozoan parasite. In the present study, we investigated B. microti infection in questing ticks in Mongolia. A total of 219 questing ticks were collected from three different Mongolian provinces (Bayan-Olgii, Khovsgol, and Selenge). Of these, 63 from Selenge were identified as Ixodes persulcatus, while the remaining 156 (from all three provinces) were identified as Dermacentor nuttalli. When the tick DNA samples were screened using a B. microti-specific nested PCR, 19 (30.2%) of the 63 I. persulcatus ticks were found to be B. microti-positive. The parasite was not detected in D. nuttalli. Subsequently, the 18S rRNA, cox1, and tufA sequences of B. microti were amplified, sequenced, and subjected to...
Palavras-chave: 18S rRNA; B. microti; Cox1; Mongolia; Ticks; TufA.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4554

Protective immunity of blood plasma fraction containing Babesia rodhaini exoantigens in mice
Provedor de dados: 19
Autores: Kamiya, Shiho; Takabatake, Noriyuki; Fujii, Kei; Okamura, Masashi; Iseki, Hiroshi; Yokoyama, Naoaki; Igarashi, Ikuo.
Babesia spp. are major hemoprotozoan parasites in animals. Exoantigens, which are soluble antigens released by the parasites during their asexual development, have been successfully applied for the development of a vaccine against babesiosis. In this study, we examined the ability of exoantigens to induce protective immunity in mice against a rodent Babesia parasite, Babesia rodhaini. The BALB/c mice immunized with the blood plasma fraction of B. rodhaini-infected mice (B. rodhaini exoantigens) showed a significant decrease in the lethal rate when challenged with B. rodhaini. Western blot analysis with anti-B. rodhaini exoantigen immune serum specifically detected at least eleven proteins in the lysate of B. rodhaini-infected erythrocytes. Next, a cDNA...
Palavras-chave: Babesiosis; Babesia rodhaini; Exoantigen; Protective effect; Mice.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/149

Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis
Provedor de dados: 19
Autores: Thekisoe, Oriel M. M.; Kuboki, Noritaka; Nambota, Andrew; Fujisaki, Kozo; Sugimoto, Chihiro; Igarashi, Ikuo; Yasuda, Jun; Inoue, Noboru.
In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that...
Palavras-chave: LAMP; Trypanosomosis; Trypanosoma brucei brucei; T. b. rhodesiense; T. b. gambiense; T. congolense; T. cruzi; T. evansi.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1045

Changes in immune serum of toxoplasma gondii following administration of T. gondii lysate antigen
Provedor de dados: 19
Autores: Yoda, Tomoko; Sakurai, Haruhisa; Igarashi, Ikuo; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; 依田, 知子; 櫻井, 治久; 五十嵐, 郁男; 小俣, 吉孝; 齊藤, 篤志; 鈴木, 直義.
Palavras-chave: Toxoplasma; Serum components; Bovine; Toxoplasma lysate antigen; Lymphokines.
Ano: 1990
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1569

ウマバベシア病に対する国際標準診断法の開発
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
2001年度～2004年度科学研究費補助金基盤研究(A)(2)研究成果報告書13356007
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2287

実験的赤血球内寄生原虫症の感染防御に関する基礎的研究
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
平成5年度科学研究費補助金（一般研究（C））研究成果報告書030660318
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2284

ウシ及びウマ・バベシア原虫 in vitro 長期培養法の確立とその応用に関する研究
Provedor de dados: 19
Autores: 五十嵐, 郁男; Igarashi, Ikuo.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2283

Babesia bovis: Subcellular localization of host erythrocyte membrane components during their asexual growth
Provedor de dados: 19
Autores: Okamura, Masashi; Yokoyama, Naoaki; Takabatake, Noriyuki; Okubo, Kazuhiro; Ikehara, Yuzuru; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
In the present study, the subcellular localization of the host red blood cell (RBC) membrane components, the alpha 2-3-linked sialic acid (SA) residues and the lipid bilayer, was observed during the asexual growth of Babesia bovis using two erythrocyte probes, the SA-specific lectin (MALII) and the lipophilic fluorescent (PKH2) probes, respectively. In confocal laser scanning microscopy with MALII, the SA residues on the surface of parasitized RBCs appeared to accumulate into the intracellular parasites as the parasites matured as well as to remain on the surface of extracellular parasites. Furthermore, when PKH2-labeled RBCs were infected with B. bovis, PKH2 signals were also observed around both the intracellular and the extracellular parasites,...
Palavras-chave: Babesia bovis (B. bouis); SA; MALII; RBC; RT; PBS; Babesia equi (B. equi); PV.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1040

Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay with recombinant Toxoplasma gondii matrix antigen 1
Provedor de dados: 19
Autores: Tumurjav, Buyannemekh; Terkawi, Mohamad Alaa; Zhang, Houshuang; Zhang, Guohong; Jia, Honglin; Goo, Youn-Kyoung; Yamagishi, Junya; Nishikawa, Yoshifumi; Igarashi, Ikuo; Sugimoto, Chihiro; Xuan, Xuenan.
Toxoplasma gondii matrix antigen 1 (TgMAG1), known as the 65-kDa protein, which is abundantly expressed in both bradyzoites and tachyzoites, was evaluated as a candidate for the development of a diagnostic reagent for ovine toxoplasmosis. The TgMAG1 gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST),and the recombinant TgMAG1 (rTgMAG1) was tested in an enzyme-linked immunosorbent assay (ELISA). The ELISA with rTgMAG1 showed a highly specific reaction with sera from mice experimentally infected with T. gondii but not with the closely related Neospora caninum. The antibodies to TgMAG1 were detectable from the acute to the chronic infectious stages in a mouse model. A total of 175 serum samples collected from sheep...
Palavras-chave: ELISA; Mongolia; Sheep; TgMAG1; Toxoplasma gondii.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3018

A PRELIMINARY STUDY FOR ELIMINATION OF CONTAMINATED BABESIA MICROTI FROM THE MODEL OF HUMAN PLASMA FRACTION
Provedor de dados: 19
Autores: Nishisaka, M.; Igarashi, Ikuo; Fujisaki, Kozo; Miyahara, T.; Ogata, Y.; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Babesia microti; Human plasma fraction; Filtration.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/629

Role of heat shock proteins in protective immunity against infection with Toxoplasma Gondii
Provedor de dados: 19
Autores: Suzuki, Naoyoshi; Nagasawa, Hideyuki; Hisaeda, Hajime; Igarashi, Ikuo; Horiuchi, Motohiro; Inoue, Noboru; Himeno, Kunisuke.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1061

Studies Related to Immunosuppression in Mice with Chronic Toxoplamosis
Provedor de dados: 19
Autores: Ito, Masanari; Igarashi, Ikuo; Fujita, Yukitoki; Omata, Yoshitaka; Saito, Atsushi; Nakagawa, Michio; Claveria, Florencia G.; Suzuki, Naoyoshi.
Palavras-chave: Toxoplasma; Cysts; Mice; Immunosuppression; Immunoregulator; Obiopeptide-1.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/193

Continuous in vitro Cultivation of Babesia ovata
Provedor de dados: 19
Autores: Igarashi, Ikuo; Avarzed, Avgaandorjiin; Tanaka, Tetsuya; Inoue, Noboru; Ito, Masanari; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi.
Palavras-chave: Babesia ovata; In vitro culture; Low oxygen atmosphere; Cryopreservation.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/212

Artesunate, a potential drug for treatment of Babesia infection
Provedor de dados: 19
Autores: Goo, Youn-Kyoung; Terkawi, M. Alaa; Jia, Honglin; Aboge, G. Oluga; Ooka, Hideo; Kim, Suk; Igarashi, Ikuo; Nishikawa, Yoshifumi; Xuan, Xuenan.
The effect of artesunate, a water-soluble artemisinin derivative, against Babesia species, such as Babesia bovis, Babesia gibsoni, and Babesia microti was studied. Cultures of B. bovis and B. gibsoni were treated with 0.26 μM, 2.6 μM, 26 μM, and 260 μM artesunate, and the growth-inhibitory effect was shown in over 2.6 μM artesunate in day 4 and day 3 post-subculture for B. bovis and B. gibsoni, respectively, in dose-dependent manner. In vivo experiment for B. microti, strong inhibition effects were observed in mouse groups treated with over 1.0 mg/kg body weight of artesunate on day 9 and 10 post-infection. These results suggest that artesunate could be a potential drug for Babesia infection.
Palavras-chave: Artesunate; Babesia bovis; Babesia gibsoni; Babesia microti.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2820

Sequence Analysis of Three Major Antigens (P30, P23 and P22) of Virulent and Avirulent Strains of Toxoplasma gondii
Provedor de dados: 19
Autores: Maki, Yoshiyuki; Seng, Seyha; Kato, Mihoko; Hoshi, Yukihiro; Igarashi, Ikuo; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Toxoplasma gondii; SAG-1 (P30); CDNA.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/250

Detection of Antibodies to Babesia equi by The Latex Agglutination Test with Recombinant Merozoite Antigen-2
Provedor de dados: 19
Autores: Tanaka, Tetsuya; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; EMA-2; Baculovirus; Latex agglutination test.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/329

NITRIC OXIDE IS INVOLVED IN EARLY HOST DEFENSE AGAINST A PRIMARY INFECTION WITH BABESIA MICROTI IN MICE
Provedor de dados: 19
Autores: Remer, Katharina; Igarashi, Ikuo; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Babesia microti; Nitric oxide; INOS-/- mice; Aminoguanidine; Parasitemia; Hematocrit; Interferon-γ.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/633

Purification of Merozoites from In Vitro Cultured Babesia caballi- Infected Equine Erythrocytes
Provedor de dados: 19
Autores: Ikadai, Hiromi; Sato, Masumi; Avarzed, Abgaandorjiin; Nagasawa, Hideyuki; Igarashi, Ikuo; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; 長澤, 秀行; 五十嵐, 郁男.
Palavras-chave: Purification; Merozoite; Babesia caballi.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/272

Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B-microti infection in mice
Provedor de dados: 19
Autores: Terkawi, M. Alaa; Jia, Honglin; Zhou, Jinlin; Lee, Eung-goo; Igarashi, Ikuo; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan.
Babesia gibsoni ribosomal phosphoprotein PO (BgP0) was identified as an immunodominant cross-reactive antigen with B. microti. The BgPO gene is a single copy with a predicted open reading frame of 942 bp and 314 amino acids. The BgP0 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice with the recombinant BgPO showed a specific band with a 34-kDa molecular mass in the extracts of B. gibsoni and B. microti merozoites. Furthermore, the intraperitoneal (i.p.) immunization of rBgP0 and Freund's adjuvant induced strong Immoral response consisting of mixed immunoglobulins IgG1 and IgG2a in BALB/c mice. Following the challenge with B. microti, these mice delayed the onset of parasites and significantly reduced...
Palavras-chave: B. gibsoni; B. microti; Ribosomal phosphoprotein P0; Cross-reactive antigen.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1036

IN VITRO CULTURE OF THEILERIA ORIENTALIS SERGENTI, AND THE USE OF THE BOVINE-RED BLOOD CELL-SCID MOUSE MODEL IN-AID OF CULTIVATION
Provedor de dados: 19
Autores: Ogata, Takashi; Tsukamoto, Daisuke; Sato, Masumi; Kamio, Tsugihiko; Terada, Yutaka; Igarashi, Ikuo; Claveria, Florencia G.; Fujisaki, Kozo; 五十嵐, 郁男.
Palavras-chave: Adult bovine serum; Bo-RBC SCID mice; In vitro; Theileria orientalis sergenti.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/628

Inhibitory Effects of Pepstatin A and Mefloquine on the Growth of Babesia Parasites
Provedor de dados: 19
Autores: Munkhjargal, Tserendorj; AbouLaila, Mahmoud; Mohamad Alaa Terkawi; Sivakumar, Thillaiampalam; Ichikawa, Madoka; Davaasuren, Batdorj; Nyamjargal, Tserendorj; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
We evaluated the inhibitory effects of pepstatin A and mefloquine on the in vitro and in vivo growths of Babesia parasites. The in vitro growth of Babesia bovis, B. bigemina, B. caballi, and B. equi was significantly inhibited (P &lt; 0.05) by micromolar concentrations of pepstatin A (50% inhibitory concentrations = 38.5, 36.5, 17.6, and 18.1 μM, respectively) and mefloquine (50% inhibitory concentrations = 59.7, 56.7, 20.7, and 4 μM, respectively). Furthermore, both reagents either alone at a concentration of 5 mg/kg or in combinations (2.5/2.5 and 5/5 mg/kg) for 10 days significantly inhibited the in vivo growth of B. microti in mice. Mefloquine treatment was highly effective and the combination treatments were less effective than other treatments....
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3829

Detection of Collagen-cross Reactive Antigenic Components in Toxoplasma gondii
Provedor de dados: 19
Autores: Omata, Yoshitaka; Inoue, Noboru; Yonematsu, Kunio; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Collagen; Cross-reactive antigens; Toxoplasma gondii.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/223

Babesia bovis: Effects of cysteine protease inhibitors on in vitro growth
Provedor de dados: 19
Autores: Okubo, Kazuhiro; Yokoyama, Naoaki; Govind, Yadav; Alhassan, Andy; Igarashi, Ikuo.
In the present study, we examined the effects of four kinds of cysteine protease inhibitors (E64, E64d, leupeptin, and ALLN) on the in vitro asexual growth of Babesia bovis. Of these, only the lipophilic inhibitors, E64d and ALLN, were found to effectively inhibit the growth of B. bovis. In further experiments, E64d, but not ALLN, significantly suppressed the parasite’s invasion of host erythrocytes, while both chemicals, especially ALLN, inhibited the parasite’s replication within the infected erythrocytes. These data suggested the presence of cysteine protease(s) derived from B. bovis, in which the protease(s) would play important roles in the erythrocyte invasion and/or replication processes of the parasite.
Palavras-chave: Babesia bovis; Cysteine protease; Inhibitor; Invasion; Replication.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1041