Consulta: subjectFacets:"Bivalve"
Registros recuperados: 74
Data/hora: 10/06/2024 17:20:59

Culturally significant fisheries: keystones for management of freshwater social-ecological systems
Provedor de dados: 7
Autores: Noble, Mae; Fenner School of Environment and Society, The Australian National University; mae.noble@anu.edu.au; Duncan, Phil; Gamilaroi Traditional Owner, NSW Aboriginal Land Council; phil.duncan@alc.org.au; Perry, Darren; Murray Lower Darling Rivers Indigenous Nations; ngintait@gmail.com; Prosper, Kerry; Paq'tnekek Mi'kmaq First Nations; kerryp@paqtnkek.ca; Rose, Denis; Gunditj Mirring Traditional Owners Aboriginal Corporation; Denis@gunditjmirring.com; Schnierer, Stephan; School of Environment, Science and Engineering, Southern Cross University; stephan.schnierer@scu.edu.au; Tipa, Gail; Tipa and Associates Ltd.; gttipa@vodafone.co.nz; Williams, Erica; National Institute of Water and Atmospheric Research; Erica.Williams@niwa.co.nz; Woods, Rene; National Cultural Flows Program; Murray Lower Darling River Indigenous Nations; woodsre83@gmail.com; Pittock, Jamie; Fenner School of Environment and Society, The Australian National University; jamie.pittock@anu.edu.au.
Indigenous peoples of North America, Australia, and New Zealand have a long tradition of harvesting freshwater animals. Over generations of reliance and subsistence harvesting, Indigenous peoples have acquired a profound understanding of these freshwater animals and ecosystems that have become embedded within their cultural identity. We have identified trans-Pacific parallels in the cultural significance of several freshwater animal groups, such as eels, other finfish, bivalves, and crayfish, to Indigenous peoples and their understanding and respect for the freshwater ecosystems on which their community survival depends. In recognizing such cultural connections, we found that non-Indigenous peoples can appreciate the deep significance of freshwater animals...
Tipo: Peer-Reviewed Synthesis
Palavras-chave: Adaptive freshwater management; Aquatic resources; Bivalve; Comanagement; Crayfish; Cultural keystone species; Eel; Indigenous ecological knowledge; Indigenous water rights; Lamprey; Salmon; Social-ecological resilience.
Ano: 2016

Space-time variation of the relative abundance of Limnoperna fortunei in deep zones of São Gonçalo Channel, Rio Grande do Sul, Brazil
Provedor de dados: 76
Autores: Lopes,Michelle; Vieira,João.
This work describes the spatial-temporal variation of the relative abundance and size of Limnoperna fortunei (Dunker, 1857) collected in São Gonçalo Channel through bottom trawl with a 0.5 cm mesh, at depths between 3 and 6 m. The estimative of mean relative abundance (CPUE) ranged from 2,425.3 individuals per drag (ind./drag) in the spring to 21,715.0 ind./drag in the fall, with an average of 9,515.3 ind./drag throughout the year. The estimated mean density of L. fortunei for the deep region of São Gonçalo Channel ranged from 1.2 to 10.3 ind./m², and it was recorded a maximum density of 84.9 ind./m² in the fall of 2008. The method of sampling using bottom trawl enabled the capture of L. fortunei under the soft muddy bottom of the channel, in different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater invasion; Frequency of sizes; Golden mussel; Bivalve.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212012000400002

Analysis of shell colour determinism in the Manila clam Ruditapes philippinarum
Provedor de dados: 5
Autores: Peignon, Jean-marie; Gerard, Andre; Naciri, Yamama; Ledu, Christophe; Phelipot, Pascal.
The Manila clam presents a large polymorphism for shell colours and patterns. No experiment has been conducted to study the determinism of such a polymorphism which has been well known for a long time. The aim of this study was firstly to determine the genetic variation for shell colour and secondly to produce some homozygous lines for the shell colouring. These lines could then be used as controls for laboratory experiments. The colouring is defined by three characters: asymmetry, ornamentation and hue. Clams with asymmetrical colouring are not fully pigmented, except for a posterior band on the left valve. Clams with symmetrical colouring are fully pigmented on the two valves. Four cross series were conducted between the different characters: Asymmetry...
Tipo: Text
Palavras-chave: Mollusque; Bivalve; Palourde; Ruditapes philippinarum; Génétique; Hérédité mendélienne; Coloration; Asymétrie; Mollusc; Bivalve; Manila clam; Ruditapes philippinarum; Genetics; Mendelian inheritance; Colouration; Asymmetry.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/publication-3204.pdf

Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)
Provedor de dados: 5
Autores: Fuhrmann, Marine; Petton, Bruno; Quillien, Virgile; Faury, Nicole; Morga, Benjamin; Pernet, Fabrice.
Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the...
Tipo: Text
Palavras-chave: Bivalve; Herpesviridae; Infection; Disease transmission; Risk analysis.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00350/46100/45781.pdf

Estimating Symbiont Abundances and Gill Surface Areas in Specimens of the Hydrothermal Vent Mussel Bathymodiolus puteoserpentis Maintained in Pressure Vessels
Provedor de dados: 5
Autores: Duperron, Sebastien; Quiles, Adrien; Szafranski, Kamil M.; Leger, Nelly; Shillito, Bruce.
The hydrothermal vent mussel Bathymodiolus puteoserpentis hosts gill-associated sulfur- and methane-oxidizing bacteria which sustain host nutrition and allow it to reach high densities at various sites along the northern Mid-Atlantic Ridge. Previous studies have demonstrated that in similar dual symbioses, relative abundances of each bacterial type could change following variations in symbiont substrate availabilities. In this study, pressurized recovery and incubations in pressure vessels were used to test whether B. puteoserpentis symbionts displayed similar behavior in the presence of symbiont substrates. The relative abundances of both types of symbionts were analyzed using fluorescence in situ hybridization (FISH) and group-specific gene copy numbers...
Tipo: Text
Palavras-chave: Symbiosis; In vivo experiments; Pressurized recovery; Bivalve; Mid-Atlantic Ridge.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00484/59533/62525.pdf

Etude histoensymologique de la digestion chez Ruditapes philippinarum
Provedor de dados: 5
Autores: Hily, A.
Examinations of alimentary tract and digestive tubules after feeding give evidence of continuous digestive process in juveniles of Ruditapes philippinarum . Histoenzymologic study of digestive tract suggests important intracellular enzymatic activities in stomacal epithelium, digestive gland and intestinal cells. Main part of intracellular digestion occurred in digestive cells by lysosyme associated with vacuolar system.
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Bivalvia; Digestive system; Enzymatic activity; Histology; Digestive glands; Digestion; Enzymes digestives; Digestion; Bivalve.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1180.pdf

Connectivities with shellfish farms and channel rivers are associated with mortality risk in oysters
Provedor de dados: 5
Autores: Gangnery, Aline; Normand, Julien; Duval, Cyrielle; Cugier, Philippe; Grangeré, Karine; Petton, Bruno; Petton, Sebastien; Orvain, Francis; Pernet, Fabrice.
Oyster diseases have major consequences on fisheries and aquaculture. In France, young Pacific oysters Crassostrea gigas are severely hit by the ostreid herpesvirus, whereas adults suffer mortalities presumably caused by pathogenic bacteria. Here we investigated the origin and spread of mortalities that affect both young and adult oysters, and we identified and compared their risk factors. Mortality was monitored in 2 age classes of oysters deployed in early spring at 39 sites spread over a 37 km2 surface area inside and outside of shellfish farms. Environmental data obtained from numerical modelling were used to investigate risk factors. Mortality of young oysters associated with ostreid herpesvirus occurred in the oyster farming area. Hydrodynamic...
Tipo: Text
Palavras-chave: Aquaculture; Bivalve; Disease; Epidemiology and health; Ecological modelling; Hydrodynamic connectivity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00588/69983/67896.pdf

Contribution à l'étude du système neuroendocrine de la moule Mytilus edulis (Mollusca: Bivalvia)
Provedor de dados: 5
Autores: Kellner-cousin, Kristell.
Dans le cadre de ce travail, l'étude du système neuroendocrine de la moule Mytilus edulis a été envisagée sous plusieurs aspects qui définissent les quatre grandes parties de la thèse.  Dans une première partie est décrit le travail de préparation et de caractérisation d'anticorps monoclonaux (AcMc) spécifiques de ganglions cérébroïdes (GC) de Mytilus edulis. Le développement de techniques de préparation d'AcMc dirigés contre des antigènes non purifiés a en effet permis de produire un ensemble de réactifs de type AcMc dirigés contre différents antigènes issus d'un broyat de GC de moule. La spécificité de ces AcMc a été étudiée en microscopie optique puis électronique selon des techniques immunocytochimiques (immunopéroxydase indirecte et immunogold). La...
Tipo: Text
Palavras-chave: Mollusque; Bivalve; Mytilus edulis; Cellules neurosécrétrices; Système nerveux; Système immunitaire; Controle de la croissance; Insuline; Anticorps monoclonaux; Immunocytocbimie; ELISA; PCR; Banque d'ADNc.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00109/22019/19642.pdf

La bonamiose de l' huître plate Ostrea edulis. Bilan des connaissances actuelles, analyse des données collectées en France de 1989 à 2004, mesures de lutte et perspectives
Provedor de dados: 5
Autores: Blanchet, Estelle.
Bonamiosis, favoured by change of temperature and stress, causes mortalities in the adult flat oyster Ostrea edulis in the majority of European oyster farmers. The life cycle of this Haplosporidia protozoan Bonamia ostreae is unknown, it is not dttectable during the five mon th latent period. Because of the Jack of sensibility of reference techniques, histology and tissue smear, further use of PCR assays or HCM-CC, in particular, is recommended. Analysis of data collected in France from 1989 to 2004 shows the absence of simple prediction methods and an apparent cyclicity of the disease. Transfer restriction and control of boat hulls can protect uninfected areas. Reducing stress and density, commercialising earlier can limit losses in infected areas. The...
Tipo: Text
Palavras-chave: Bivalve; Bonamia ostreae; Epidémiologie; Haplosporidia; Huître plate; Lutte; Ostrea edulis; Protozoaire; Surveillance; Bonamia ostreae; Control; Epidemiology; Flat oyster; Haplosporidia; Ostrea edulis; Protozoan; Survey.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00451/56235/57795.pdf

Optimization of a survey using spatially balanced sampling: a single-year application of clam monitoring in the Arcachon Bay (SW France)
Provedor de dados: 5
Autores: Kermorvant, Claire; Caill-milly, Nathalie; D'Amico, Frank; Bru, Noelle; Sanchez, Florence; Lissardy, Muriel; Brown, Jennifer.
Bivalves are important components of benthic marine and freshwater ecosystems throughout the world. One of the most exploited bivalves used for human consumption is manila clam (Venerupis philippinarum). In Arcachon Bay (SW France), commercial fishers and scientists have developed a monitoring survey to estimate clam stocks to assist in implementing a sustainable management strategy. The survey design that is currently used is based on standard stratified random sampling (StRS). The survey has been undertaken every 2 years since 2006. Each survey costs approximately (sic) 50 000, with funding provided by similar to 20% of the commercial fishers. The survey is quite expensive, given that this resource is managed mostly at a regional level. In 2016 for...
Tipo: Text
Palavras-chave: Venerupis philippinarum; Bivalve; Simulation; Survey; Arcachon Bay; GRTS.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00410/52102/52808.pdf

Viruses infecting marine molluscs
Provedor de dados: 5
Autores: Arzul, Isabelle; Corbeil, Serge; Morga, Benjamin; Renault, Tristan.
Although a wide range of viruses have been reported in marine molluscs, most of these reports rely on ultrastructural examination and few of these viruses have been fully characterized. The lack of marine mollusc cell lines restricts virus isolation capacities and subsequent characterization works. Our current knowledge is mostly restricted to viruses affecting farmed species such as oysters Crassostrea gigas, abalone Haliotis diversicolor supertexta or the scallop Chlamys farreri. Molecular approaches which are needed to identify virus affiliation have been carried out for a small number of viruses, most of them belonging to the Herpesviridae and birnaviridae families. These last years, the use of New Generation Sequencing approach has allowed increasing...
Tipo: Text
Palavras-chave: Molluscs; Viruses; Diseases; Herpesviridae; Bivalve; Abalone.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00371/48206/48319.pdf

DEB2009 Symposium : Dynamic Energy Budget, 30 years of research for metabolic organization, Book of abstracts
Provedor de dados: 5
We are pleased to welcome you to at the first Symposium on Metabolic organization in Brest, 19-22 April, 2009. This conference is being organized jointly by Ifremer (French Institute for the Research and Exploitation of the Sea), the Department of Theoretical Biology at Vrije Universiteit Amsterdam, the Universities of Brest (UBO), Marseille (LMGEM-UMR CNRS 6117), Caen (UMRPE2M, LBBM) and Lisbon (Instituto Superior Técnico), the IRD (French Research Institute for Development), the INRA (French National Institute for Agricultural Research), the NIOZ (RoyalNetherlands Institute for Sea Research), the IMARES Wageningen (Institute for Marine Resources and Ecosystem Studies), as all these research teams are involved in research on DEB theory. You are 81 persons...
Tipo: Text
Palavras-chave: Ecology; Bivalve; Chemistry; Ecotoxicology; Physiology; Dynamic energy budget; Biology.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/acte-6790.pdf

Adaptation du métabolisme respiratoire de l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Le Moullac, Gilles.
Summer mortality of the oyster result from the interaction of many factors. A respiratory assumption proposes a link between the supposed causes of the energy state, the cultivation methods and the medium. This work is focused towards the search for specific metabolic markers of environmental stresses. The cDNA of genes coding for PK and PEPCK were cloned and sequenced in order to detect their regulation level. The sediment nearness affects the state of energy reserves, the metabolic pathways of energy production implying a regulation of the PK. Face to the dissolved oxygen decline, oysters were oxyregulator but with a low regulation ability. In hypoxia, glycolysis was slowed down. PK activity was inhibited resulting from an increase in alanine sensitivity...
Tipo: Text
Palavras-chave: Régulation; Enzyme; Expression génique; Anoxie; Bivalve.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-4315.pdf

Viruses of bivalve shellfish
Provedor de dados: 5
Autores: Renault, Tristan.
Bivalve molluscs are filter feeders and as a consequence they may bioaccumulate in their tissues viruses that infect humans and higher vertebrates. However, there have also been described mortalities of bivalve molluscs associated with viruses belonging to different families. Mass mortalities of adult Portuguese oysters, Crassostrea angulata, among French livestocks (between 1967 et 1973) were associated with irido-like virus infections. Herpesviruses were reported in the eastem oyster, Pacific oyster and European flat oyster and lately in scallops and clams. Disseminated neoplasia, a proliferative cell disorder of the circulatory system of bivalves, although of uncertain aetiology, has been suggested to be caused by retroviral infections. Other viruses...
Tipo: Text
Palavras-chave: Infection; Virus; Mollusc; Bivalve; Infection; Virus; Mollusque; Bivalve.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2925.pdf

Does trace element composition of bivalve shells record utra-high frequency environmental variations?
Provedor de dados: 5
Autores: Poitevin, Pierre; Chauvaud, Laurent; Pecheyran, Christophe; Lazure, Pascal; Jolivet, Aurélie; Thebault, Julien.
Saint-Pierre and Miquelon (SPM) is a small archipelago where instrumental measures based on water column velocity and temperature profiles compiled comprehensive evidence for strong near-diurnal (25.8h) current and bottom temperature oscillations (up to 11.5 °C) which is possibly the largest ever observed — at any frequency — on a stratified mid-latitude continental shelf. The main objective of our study was to identify if Placopecten magellanicus can record on its shell these high frequency environmental variations. To this end, we have tried to identify proxies for water temperature and food availability through development of a new ultra-high resolution LA-ICPMS analyses method capable of resolving shell surface elemental composition with a 10 μm...
Tipo: Text
Palavras-chave: Ultra-high resolution LA-ICPMS; Placopecten magellanicus; Shell chemistry; Trace elements; Environmental change; Bivalve; Environmental proxies; North Atlantic; Saint-Pierre and Miquelon; Coastal trapped wave.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00612/72400/71345.pdf

Morphological Trends of Four Manila Clam Populations (Venerupis philippinarum) on the French Atlantic Coast: Identified Spatial Patterns and Their Relationship to Environmental Variability
Provedor de dados: 5
Autores: Caill-milly, Nathalie; Bru, Noelle; Barranger, Melanie; Gallon, Laurent; D'Amico, Franck.
For species' dynamics highly influenced by environmental conditions, sustainable management for conservation and/or exploitation purposes requires the ability to segregate natural processes from anthropogenic impacts. In this context, identification of phenotypic variations in morphological patterns, and clarification of the mechanisms involved, constitute useful tools to identify adequate management measures from Europe-wide to local conditions. Although such approaches are already used effectively in the management of fish stocks, they are more recent for bivalve stocks, which are also good candidates for such management tools. The current study examines morphological patterns in the Manila clam (Venerupis philippinarum) through morphometric studies of 4...
Tipo: Text
Palavras-chave: Shell shape; Linear morphometric methods; Contour detection; Environmental effect; Linear multilevel model; Bivalve; Atlantic Ocean; Venerupis philippinarum.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00225/33605/32004.pdf

Remote Sensing-Driven Pacific Oyster (Crassostrea gigas) Growth Modeling to Inform Offshore Aquaculture Site Selection
Provedor de dados: 5
Autores: Palmer, Stephanie C. J.; Gernez, Pierre M.; Thomas, Yoann; Simis, Stefan; Miller, Peter I.; Glize, Philippe; Barillé, Laurent.
Aquaculture increasingly contributes to global seafood production, requiring new farm sites for continued growth. In France, oyster cultivation has conventionally taken place in the intertidal zone, where there is little or no further room for expansion. Despite interest in moving production further offshore, more information is needed regarding the biological potential for offshore oyster growth, including its spatial and temporal variability. This study shows the use of remotely-sensed chlorophyll-a and total suspended matter concentrations retrieved from the Medium Resolution Imaging Spectrometer (MERIS), and sea surface temperature from the Advanced Very High Resolution Radiometer (AVHRR), all validated using in situ matchup measurements, as input to...
Tipo: Text
Palavras-chave: Satellite image; Time series; Bivalve; Dynamic energy budget; Growth modeling; MERIS; AVHRR; Marine spatial planning.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00605/71722/70185.pdf

Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat
Provedor de dados: 5
Autores: Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco.
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides...
Tipo: Text
Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf

Transmission expérimentale du virus de type herpes chez l'huître creuse, Crassostrea gigas
Provedor de dados: 5
Autores: Arzul, Isabelle.
Au cours de l'été 1991, d'importantes mortalités de larves d'huître creuse, Crassostrea gigas, ont été observées dans des écloseries, simultanément en France (Comps et Cochennec, 1993) et en Nouvelle-Zélande (Hine &amp; al., 1992).  Les examens histologiques ainsi que des examens réalisés en microscopie électronique à transmission d'échantillons de larves présentant des mortalités ont permis de détecter la présence de particules virales associées à des lésions cellulaires.  D'après la morphologie, la taille, les localisations intranucléaires et cytoplasmiques du virus, il est possible d'envisager son appartenance à la famille des Herpesviridae.  De nouvelles mortalités sporadiques ont été observées au cours des étés suivants sur des lots de larves élevées...
Tipo: Text
Palavras-chave: Virus; Herpesviridae; Bivalve; Crassostrea gigas; Etiologie; Epidémiologie; Diagnostic; Reproduction infection.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00106/21711/19289.pdf

Feeding rate of the oyster Crassostrea gigas in a natural planktonic community of the Mediterranean Thau Lagoon
Provedor de dados: 5
Autores: Dupuy, Christine; Vaquer, André; Lam Hoai, Thong; Rougier, Claude; Mazouni, Nabila; Lautier, Jacques; Collos, Yves; Le Gall, Solange.
The Mediterranean Thau Lagoon is an important oyster farming area in Europe. Oyster growth rates are among the highest in France, although chlorophyll a concentration is low. Previous studies have demonstrated that picophytoplankton, nano-microphytoplankton, dinoflagellates and loricate ciliates such as tintinnids are abundant. However, heterotrophic flagellates and aloricate ciliates have not been investigated. The aim of this study was to assess picophytoplankton, protist and zooplankton abundances in the Lagoon and to investigate the particular structure of the microbial food web, which may explain such paradoxical oyster growth. In oligotrophic waters in the Thau Lagoon, the picoeukaryote Ostreococcus tauri is the dominant autotrophic picoplankter,...
Tipo: Text
Palavras-chave: Trophic link; Picophytoplankton; Heterotrophic protist; Microbial food web; Thau Lagoon; Food source; Oyster; Bivalve.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-812.pdf

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm
Provedor de dados: 5
Autores: Suquet, Marc; Quere, Claudie; Mingant, Christian; Lebrun, Luc; Ratiskol, Dominique; Miner, Philippe; Cosson, Jacky.
Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed...
Tipo: Text
Palavras-chave: Spermatozoa; CASA; Maturation; Sperm motility; Bivalve; Pecten maximus.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26852/24983.pdf

Genetic structure at different spatial scales in the pearl oyster (Pinctada margaritifera cumingii) in French Polynesian lagoons: beware of sampling strategy and genetic patchiness
Provedor de dados: 5
Autores: Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre.
In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat...
Tipo: Text
Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf

Influence of low temperatures on the survival of the Pacific oyster (Crassostrea gigas) infected with Ostreid herpesvirus type 1
Provedor de dados: 5
Autores: Pernet, Fabrice; Tamayo, David; Petton, Bruno.
Mortalities of oyster seed of Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have been observed in many oyster producing countries since 2008. The objective of this study was to further investigate whether low temperature treatments can offer a viable option to mitigate oyster mortalities. An experiment was set-up to further evaluate the effect of low temperature treatments (10 and 13°C vs. 21°C) and their duration (6 d to 83 d) on the survival of oysters previously infected with OsHV-1 μVar by means of exposure to field conditions in areas where mortalities were occurring. Average survival of oysters infected with OsHV-1μVar was 71% after 83 d at low temperatures compared to only 23% in controls maintained at 21°C. During...
Tipo: Text
Palavras-chave: Bivalve; Disease; OsHV-1; Oyster; Temperature; Virus.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00259/37047/35555.pdf

Relations entre le contenu lipidique des ovocytes de Pecten maximus (Mollusque, bivalve) et les performances des larves en élevage
Provedor de dados: 5
Autores: Le Pennec, Marcel; Gueguen,.
The lipid content of spawned oocytes of Pecten maximus was studied by image analysis of semi -thin histological sections and a lipid index was defined. The female gametes were obtained by thermic stimulations of mature adults scallops from different populations after out of season conditioning or sampled in situ during the spawning season. After fertilization, the larval development was followed. The results reveal a large variability in lipid content of normal oocytes. The lipid content can be regarded as homogeneous inside a spawning but the heterogeneity is greater for wild scallops than for conditioned ones. A strong relationship appears between the lipid index and the hatching success, and the number of abnormal larvae after two days rearing. But no...
Tipo: Text
Palavras-chave: Mollusques; Bivalves; Pecten maximus; Ovocytes; Lipides; Elevages larvaires; Mollusc; Bivalve; Pecten maximus; Oocytes; Lipids; Larval development.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00022/13345/10379.pdf

Evidence for interspecies transmission of oyster herpesvirus in marine bivalves
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Lipart, Cécile; Davison, Andrew.
Since 1991, numerous herpesvirus infections associated with high mortality have been reported around the world in various marine bivalve species. In order to determine whether these infections are due to ostreid herpesvirus-l (OsHV1), a previously characterized pathogen of the Japanese oyster (Crassostrea gigas), PCR analysis was carried out on 30 samples of larvae collected from four bivalve species (C. gigas, Ostrea edulis, Ruditapes decussatus and Ruditapes philippinarum), most exhibiting mortality prior to collection. All samples were shown to be infected by OsHV1. Viral genomes in three samples of C. gigas and three of R. philippinarum that originated from the same hatchery were unusual in bearing a deletion of at least 2.8 kbp in an inverted repeat...
Tipo: Text
Palavras-chave: Bivalve; Crassostrea gigas; Herpesvirus; Japanese oyster; Transmission.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-705.pdf

Impact of environmental variability on Pinctada margaritifera life-history traits: A full life cycle deb modeling approach
Provedor de dados: 5
Autores: Sangare, Nathanael; Lo-yat, Alain; Le Moullac, Gilles; Pecquerie, Laure; Thomas, Yoann; Lefebvre, Sebastien; Le Gendre, Romain; Beliaeff, Benoit; Andréfouët, Serge.
The black-lipped pearl oyster (Pinctada margaritifera) is extensively farmed in French Polynesia to produce black pearls. For a sustainable management of marine resources, studying interactions between organisms and environment, and the associated factors and processes that will impact their life cycle and thus modulate population dynamics is a major research priority. Here, we describe black-lipped pearl oyster energy acquisition and use, and its control by temperature and food concentration within the Dynamic Energy Budget (DEB) theory framework. The model parametrization was based on literature data and a specific laboratory experiment. Model validation was carried out thanks to historical in-situ datasets and a dedicated field survey. Three theoretical...
Tipo: Text
Palavras-chave: Bivalve; Physiology; Bioenergetics; Dynamic energy budget theory; Environmental change; Pearl farming.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00613/72469/71614.pdf

Relations entre l’état d’une ressource et son exploitation via la compréhension et la formalisation des interactions de socio-écosystèmes. Application à la palourde japonaise (Venerupis philippinarum) du bassin d’Arcachon.
Provedor de dados: 5
Autores: Caill-milly, Nathalie.
Significant component of the marine benthic ecosystem, exploited bivalves are mainly located inside the coastal area, which temporal and spatial features generate natural population fluctuations and contribute to considerable biomass and demographic structure changes added to anthropogenic activities’ effects (fishing, pollution,…). Understanding of the relationships between the species, its environment and its exploitation is crucial and their formalization, including for management purposes, requires development of mathematical models that aim at describing the functioning of the system, to explain the observed phenomenon in terms of cause and effect or predict effects of new causes. Originating from venerid culture trials carried out in France in the...
Tipo: Text
Palavras-chave: Bivalve; Morphométrie; Allométrie; Dynamique des populations; Système dynamique; Gestion; Palourde japonaise; Venerupis philippinarum; Bivalve; Morphometry; Allometry; Population dynamics; Dynamic system; Management; Manila clam; Venerupis philippinarum.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00139/25034/23143.pdf

Polyploidy in the Manila clam Ruditapes philippinarum. II. Chemical induction of tetraploid embryos
Provedor de dados: 5
Autores: Diter, Alain; Dufy, Christian.
Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B. (CB). Two treatment times were found to be effective in inducing tetraplidy : 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced 64.4 ± 7.8% and 28.3 ± 2.3% tetraploid embrios, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment.
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Bivalve; Tetraploidy; Cytochalasin B.; Ruditapes philippinarum; Bivalve; Tétraploïdie; Cytochalasine B.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/publication-2753.pdf

Etude cytologique de la gamétogenèse, de la sex-ratio et du cycle de reproduction chez l'huître perlière Pinctada margaritifera (L) var. cumingii (Jameson), (mollusques, bivalves). Comparaison avec le cycle de Pinctada maculata (Gould)
Provedor de dados: 5
Autores: Thielley, Maryse.
The population of Pinctada margaritifera and Pinctada maculata lay eggs throughout the year. Temperature variations induce huge but asynchronous laying. Preferential periods of gonad maturity correspond to the season changings in both species. However. some differences between the two specics are noticed. Both species are protandric hermaphrodites. Female condition is unstable: a reversion to the male condition may be observed and is probably linked with stress. The average size for the first sexual maturation as weil as for sexual conversion are specified in P. margaritifera. The breeding conditions induce modifications in some reproductive cycle processes (maturation, sex-ratio). Some conditions suitable for reproduction may become unfavorable to...
Tipo: Text
Palavras-chave: Gamétogenèse; Cycle de reproduction; Sex-ratio; Pinctad margaritifera; Mollusque; Bivalve; Huître perlière; Gametogenesis; Reproductive cycle; Sex-ratio; Pinctada margaritifera; Mollusca; Bivalvia; Pearl oyster.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00440/55208/56677.pdf

A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum
Provedor de dados: 5
Autores: Smits, M.; Artigaud, Sebastien; Bernay, B.; Pichereau, Vianney; Bargelloni, L.; Paillard, Christine.
Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by...
Tipo: Text
Palavras-chave: Bivalve; Diseases; Resistance; Manila clam; Vibrio tapetis; Proteomics; Immune response.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00615/72670/71758.pdf

Does bivalve mucus favour the growth of their main food source, microalgae?
Provedor de dados: 5
Autores: Cognie, B; Barille, Laurent.
Bivalves, by releasing inorganic nutrients, can stimulate the growth of the primary producers on which they feed. Dissolved excretion and mineralization of biodeposits (pseudofaeces and faeces), two indirect processes related to their feeding activity, have already been identified. This study investigated whether microalgal growth is also dependent on the mucus secreted by bivalves and rejected with their biodeposits. An experimental study based on algal growth tests was conducted to determine whether the mucus coating the pseudofaeces and faeces of the oyster Crassostrea gigas (Thunberg) could stimulate the growth of nine marine microalgae: a Prasinophyceae [Tetraselmis suecica (Butcher)] and eight Diatomophyceae [Amphora sp. (Ehrenberg), Chaetoceros sp....
Tipo: Text
Palavras-chave: Bivalve; Microphyte; Mucus; Fèces; Pseudofèces; Bivalve; Microalgae; Mucus; Faeces; Pseudofaeces.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00325/43607/43926.pdf

Etude expérimentale de différentes conditions de mileu sur le comportement du parasite de la glande digestive de l'huître plate (Ostrea edulis L.)
Provedor de dados: 5
Autores: Comps, Michel.
During trials on the influence of environmental factors on the parasite of the flat oyster digestive gland, we observed that some experimental conditions including the re-immersion after a several day-exsiccation period could result in a decrease of the parasitism rate.
Tipo: Text
Palavras-chave: Glande digestive; Huître plate; Ostrea edulis; Bivalve; Pathologie; Parasitologie.
Ano: 1975
URL: http://archimer.ifremer.fr/doc/1975/acte-6168.PDF

Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure
Provedor de dados: 5
Autores: Lassudrie, Malwenn; Soudant, Philippe; Richard, Gaelle; Henry, N.; Medhioub, Walid; Da Silva, P.m.; Donval, Anne; Bunel, M.; Le Goïc, Nelly; Lambert, Christophe; De Montaudoin, X.; Fabioux, Caroline; Hegaret, Helene.
Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the...
Tipo: Text
Palavras-chave: Bivalve; Harmful algal bloom; Oxidative stress; Venerupis philippinarum; Alexandrium ostenfeldii; Perkinsus olseni.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00189/30022/28507.pdf

Bilan énergétique chez les mollusques bivalves : terminologie et méthodologie - Compte rendu du groupe de travail réuni au laboratoire national "Ecosystème conchylicole" de l'IFREMER La Tremblade (13 et 14 septembre 1984)
Provedor de dados: 5
Autores: Heral, Maurice; Lucas, Albert; Deslous Paoli, Jean-marc; Goulletquer, Philippe; Masse, Henri; LABEIM (Laboratoire de Biologie et d'Ecologie des Invertébrés Marins).
En septembre 1984, MM. HERAL et DESLOUS-PAOLI organisèrent à La Tremblade, une "réunion méthodologie expérimentale mollusques". Cette rencontre faisait suite à un bref état qui s'était engagé au Congrès de Montpellier sur les "Bases biologiques de l'Aquaculture", où il était apparu que la terminologie et la méthodologie concernant le métabolisme des bivalves devaient être clarifiées. Grâce à une large information auprès des spécialistes français de la physiologie des bivalves, la réunion à La Tremblade a réuni un grand nombre de participants très motivés. Pendant 2 jours des discussions très animées ont permis de bien comprendre les échanges entre les bivalves et leur environnement et d'y jeter un regard neuf, ce qui impliquait une analyse critique du...
Tipo: Text
Palavras-chave: Terminologie; Méthodologie; Bilan d'énergie; Physiologie; Bivalve; Terminology; Methodology; Energy budget; Physiology; Bivalve molluscs.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00014/12562/9432.pdf

Spatial compartmentalization of free radical formation and mitochondrial heterogeneity in bivalve gills revealed by live-imaging techniques
Provedor de dados: 5
Autores: Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris.
Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas...
Tipo: Text
Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf

Etude comparative de différents indices de condition chez l'huître creuse (Crassostrea gigas)
Provedor de dados: 5
Autores: Bodoy, Alain; Prou, Jean; Berthome, Jean-paul.
Among the different condition indices, some of them were compared on a population of the japanese oyster, Crassostrea gigas. The follow-up of experimental racks was performed monthly during four years. The use of dry weight in condition indices gave a more precise and more reliable representation for the condition of the oysters than the fresh weight. The sensitivity of these indices, which corresponds to their ability in revealing small changes in the physiological condition, was calculated for each indice. Those exhibiting the best sensitivity were ranked as follow: dry weight on the difference between total weight and shell weight, dry weight on intervalve volume. Other indices including the ratio between dry weight and various assessments of the volume...
Tipo: Text
Palavras-chave: Bivalve; Crassostrea gigas; Indice de qualité; Indice de condition; Crassostrea gigas; Bivalve; Condition factor; Condition index.
Ano: 1986
URL: http://archimer.ifremer.fr/doc/00000/2961/9155.pdf

Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds
Provedor de dados: 5
Autores: Knuckey, R; Brown, M; Robert, Rene; Frampton, D.
A novel technique was developed for the flocculation of marine microalgae commonly used in aquaculture. The process entailed an adjustment of pH of culture to between 10 and 10.6 using NaOH, followed by addition of a non-ionic polymer Magnafloc LT-25 to a final concentration of 0.5 mg L&amp;#8722;1. The ensuing flocculate was harvested, and neutralised giving a final concentration factor of between 200- and 800-fold. This process was successfully applied to harvest cells of Chaetoceros calcitrans, C. muelleri, Thalassiosira pseudonana, Attheya septentrionalis, Nitzschia closterium, Skeletonema sp., Tetraselmis suecica and Rhodomonas salina, with efficiencies &amp;#8805;80%. The process was rapid, simple and inexpensive, and relatively cost neutral with...
Tipo: Text
Palavras-chave: Chaetoceros; Thalassiosira; Flocculation; Concentrate; Bivalve; Algal paste.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1820.pdf

Non‐invasive functional exploration techniques for bivalves with applications to pearl oyster   Pinctada margaritifera
Provedor de dados: 5
Autores: Le Moullac, Gilles; Soyez, Claude; Lyonnard, Pierre; Chabrier, Sébastien; Milhade, Leo; Gueguen, Yannick; Beliaeff, Benoit.
Non‐invasive functional exploration techniques can provide information on different aspects of general organism functioning and, unlike lethal or invasive techniques, allow individual organisms to be monitored for as long as necessary. For bivalves, a fairly wide variety of methods and instrumental means exist allowing physiology to be assessed on‐line while keeping animals alive and intact. The current range of non‐invasive techniques for bivalves consists of systems for measuring metabolic flows and valve activity, which can be used on bivalve molluscs for as long as an individual animal's characteristics (e.g. size) make this technically feasible. In this paper, we present some of these non‐invasive techniques with applications for pearl oyster and list...
Tipo: Text
Palavras-chave: Bivalve; Filtration; Pearl rotation; Respiration; Valvometry.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00599/71161/69551.pdf

Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Bigot, Laetitia; Zatylny-gaudin, Celine; Rodet, Franck; Bernay, Benoit; Boudry, Pierre; Favrel, Pascal.
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the...
Tipo: Text
Palavras-chave: Mollusk; Bivalve; Oyster; Neuroendocrinology; Reproduction; GnRH.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00079/19003/17477.pdf

Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Petton, Bruno; De Lorgeril, Julien; Mitta, Guillaume; Daigle, Gaétan; Pernet, Fabrice; Alunno Bruscia, Marianne.
The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific pathogen-free oysters (‘recipients’) in cohabitation in the laboratory with diseased oysters that were naturally infected in the field (‘donors’). With this method, we evaluated the temporal dynamics of pathogen release in seawater and the cohabitation time necessary for disease transmission and expression. We showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation...
Tipo: Text
Palavras-chave: Aquaculture; Bivalve; Epidemiology; Health; Polymicrobial disease; OSHV-1; Pacific oyster mortality syndrome; POMS.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00507/61870/65940.pdf

Temperature modulate disease susceptibility of the Pacific oyster Crassostrea gigas and virulence of the Ostreid herpesvirus type 1
Provedor de dados: 5
Autores: Delisle, Lizenn; Petton, Bruno; Burguin, Jean Francois; Morga, Benjamin; Corporeau, Charlotte; Pernet, Fabrice.
Temperature triggers marine diseases by changing host susceptibility and pathogen virulence. Oyster mortalities associated with the Ostreid herpesvirus type 1 (OsHV-1) have occurred seasonally in Europe when the seawater temperature range reaches 16–24 °C. Here we assess how temperature modulates oyster susceptibility to OsHV-1 and pathogen virulence. Oysters were injected with OsHV-1 suspension incubated at 21 °C, 26 °C and 29 °C and were placed in cohabitation with healthy oysters (recipients) at these three temperatures according to a fractional factorial design. Survival was followed for 14 d and recipients were sampled for OsHV-1 DNA quantification and viral gene expression. The oysters were all subsequently placed at 21 °C to evaluate the potential...
Tipo: Text
Palavras-chave: Bivalve; Health; Marine disease; Mortality risk; Temperature; Virulence.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00442/55337/56847.pdf

Polyploidy in the Manila clam Ruditapes philippinarum - 1 - Chemical induction and larval performances of triploids
Provedor de dados: 5
Autores: Dufy, Christian; Diter, Alain.
An effective treatment using catochalasin B (1 mg CB/ml dimethylsulfoxyde in 1 litre of sea water) was developed to induce triploidy in the Manila clam Ruditapes philippinurum (Adams and Reeve). The percentage of induced triploidy was assessed by karyological examination of embryos and spat. The treatment was found to be optimal between 20 to 35 minutes post-insemination exposure to CB, at 25'C, and produced 75.8 ± 5.7% triploid embryos on the average over four experiments. Growth and survival of larvae to metamorphosis were recorded for two different rearings each split into two replicates. Both larval rearings showed similar survival up to settlement: 19.3 and 27.0%, on average over two replicates, in the treated batches relative to the controls. Mean...
Tipo: Text
Palavras-chave: Larval survival; Larval growth; Bivalves; Cytochalasin B; Triploidy; Sruvie larvaire; Croissance larvaire; Bivalve; Cytochalasine B; Triploïdie.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/publication-2752.pdf

Etat des connaissances sur la pathologie chez les mollusques bivalves
Provedor de dados: 5
Autores: Fouche, Dominique.
A short description of the disease causing organisms affecting bivalves is presented in this document, including the diagnosis, the oustanding research and bivalve husbandry. Mollusc populations may be safe from disease epizootics if an early prevention of disease is coupled with a strict regulatory system on transfer (according to what happens in agriculture). The species to culture may be thoroughly selected and risky populations moved. Imported stocks are strictly controlled. The researchcs's target is to improve the diagnosis tools and try to find resistant mollusc strains. Epidemiological surveys are also necessary to control diseases.
Tipo: Text
Palavras-chave: Transfer; Husbandry; Prophylatics; Bivalve; Protozoan; Virus; Bacteria; Metazoan; Transfert; Zootechnie; Prophylaxie; Bivalve; Protozoaire; Virus; Bactérie; Métazoaire.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/rapport-1923.pdf

Bacterial problems associated with scallop Pecten maximus larval culture
Provedor de dados: 5
Autores: Nicolas, Jean-louis; Corre, S; Gauthier, Gilles; Robert, Rene; Ansquer, Dominique.
Scallop Pecten maximus larvae have been cultured at the Argenton and Tinduff (Brittany, France) hatcheries with antibiotic treatment (chloramphenicol at 8 ppm) for 15 yr. Without treatment, outbreak of disease has normally occurred between Day 12 and Day 19 or sometimes earlier. A bacteriological study of larvae reared with and without antibiotic was performed over a 4 yr period. Among the collected strains, 2 clusters (C and F) of vibrios were present at high densities only in larvae cultured without treatment. One cluster (C) was routinely isolated over the 4 yr of study, while the other (F) was collected only in the third year. Their virulence with respect to scallop larvae and their lack of infectivity with respect to oyster larvae were demonstrated in...
Tipo: Text
Palavras-chave: Larvae; Bivalve; Vibrio; Scallop.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00090/20077/17716.pdf

The future is now: marine aquaculture in the anthropocene
Provedor de dados: 5
Autores: Pernet, Fabrice; Browman, Howard I.
Aquaculture now produces more seafood than wild capture fisheries and this production is expected to at least double by 2050. Representing almost half of global production, marine aquaculture will contribute to sustainably feeding the growing humanity. However, climate change will undoubtedly challenge the future growth of marine aquaculture. Temperature and sea-level rise, shifts in precipitation, freshening from glacier melt, changing ocean productivity, and circulation patterns, increasing occurrence of extreme climatic events, eutrophication, and ocean acidification are all stressors that will influence marine aquaculture. The objective of this themed article set was to bring together contributions on the broad theme of the potential impacts,...
Tipo: Text
Palavras-chave: Adaptation; Bivalve; Blue economy; Carbon; Climate change; Marine diseases; Mitigation; Ocean acidification; Plasticity; Salmon; Seaweed; Sustainability.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00682/79406/81951.pdf

Infectious diseases in oyster aquaculture require a new integrated approach
Provedor de dados: 5
Autores: Pernet, Fabrice; Lupo, Coralie; Bacher, Cedric; Whittington, Richard J.
Emerging diseases pose a recurrent threat to bivalve aquaculture. Recently, massive mortality events in the Pacific oyster Crassostrea gigas associated with the detection of a microvariant of the ostreid herpesvirus 1 (OsHV-1 µVar) have been reported in Europe, Australia and New Zealand. Although the spread of disease is often viewed as a governance failure, we suggest that the development of protective measures for bivalve farming is presently held back by the lack of key scientific knowledge. In this paper, we explore the case for an integrated approach to study the management of bivalve disease, using OsHV-1 as a case study. Reconsidering the key issues by incorporating multidisciplinary science could provide a holistic under-standing of OsHV-1 and...
Tipo: Text
Palavras-chave: Bivalve; Disease management; Epidemiology; Multidisciplinary; Risk.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00314/42537/41912.pdf

Aspects économiques et techniques de la perliculture en Polynésie Française. Contribution à l'étude de l'alimentation de l'huître perlière Pinctada margaritifera
Provedor de dados: 5
Autores: Hautefeuille, Fanny.
Cultured black pearls are now the tirst export value of French Polynesia. This fast growing activity encourage the retum of the islanders to their native islands and the revival of the economy of the archipelagoes. However, the importance of the development induced sorne technical and economical problems. New reglementations are needed to organize the market and stabilize the black pearl price, the quotation of which is falling since 1990. After the important mortalities which occured in 1985-1 986, the mother of pearl general investigation program was set up. Il aims at the study of the biology and the ecology of the mother of pearl Pinclada margaritifera. The study of the nutrition of this oyster is part of this program and will contribute to the...
Tipo: Text
Palavras-chave: Perliculture; Polynésie Française; Problèmes; Bivalve; Huître perlière; Pinctada margaritifera; Bilan énergétique; Contenu stomacal; Assimilation; Conover; Perliculture; French Polynesia; Problems; Bivalvia; Pearl oyster; Pinctada margaritifera; Energetic balance; Stomacal contents; Assimilation; Conover.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00424/53540/54397.pdf

Remodeling of membrane lipids in gills of adult hard clam Mercenaria mercenaria during declining temperature
Provedor de dados: 5
Autores: Parent, G; Pernet, Fabrice; Tremblay, R; Sevigny, J; Ouellette, M.
In a previous study, a major remodeling of lipids, consistent with the theory of homeoviscous adaptation, was observed in the juvenile hard clam Mercenaria mercenaria during a temperature reduction from 24 to -1 degrees C. In addition, the lipid remodeling varied between genetically distinct lines of hard clams. The present study examined whether adult hard clams originating from different locations and a selectively bred variety show differences related to their genetic characteristics in the remodeling of lipids that normally occur during decreasing temperatures. Wild hard clams from 4 locations in Atlantic Canada and the selectively bred M mercenaria var. notata were held at an aquaculture growout site located at the northern distribution limit of the...
Tipo: Text
Palavras-chave: Sterol; Phospholipid; Membrane lipid; Intraspecific variation; Food; Fatty acid; Bivalve; Allozymes; Acclimatization.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4743.pdf

Etude des interactions hôte/virus chez l’huître creuse Crassostrea gigas et son virus Ostreid herpesvirus 1
Provedor de dados: 5
Autores: Segarra, Amelie.
Ostreid herpesvirus type 1 (OsHV-1) can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas, in France and all around the world. Differences in terms of mortality among infected animals were reported suggesting a genetic basis of the susceptibility to the OsHV-1 infection. In this context, the main objective of this thesis was to understand the interactions between Pacific oysters and OsHV-1, in particular, the molecular basis of the viral cycle. Results showed that the virus is able to replicate in the host regardless of its stage of development or its susceptibility. However, multiplication kinetics is faster in the most susceptible individuals compared to less susceptible ones. After an active replication phase, it...
Tipo: Text
Palavras-chave: Bivalves; Huître creuse; Crassostrea gigas; Mortalités; OsHV-1; Gènes viruax; Interaction hôte-virus; Transcriptome; Sensibilité; PCR en temps réel; Bivalve; Pacific oyster; Crassostrea ggias; Mortality; OsHV-1; Viral gene; Interaction host-virus; Transcriptome; Susceptibility; Real-time PCR.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00252/36297/34826.pdf

Low pH reduced survival of the oyster Crassostrea gigas exposed to the Ostreid herpesvirus 1 by altering the metabolic response of the host
Provedor de dados: 5
Autores: Fuhrmann, Marine; Richard, Gaëlle; Quere, Claudie; Petton, Bruno; Pernet, Fabrice.
Environmental change in the marine realm has been accompanied by emerging diseases as new pathogens evolve to take advantage of hosts weakened by environmental stress. Here we investigated how an exposure to reduced seawater pH influenced the response of the oyster Crassostrea gigas to an infection by the Ostreid herpesvirus type I (OsHV-1). Oysters were acclimated at pH 8.1 or pH 7.8 and then exposed to OsHV-1. Their survival was monitored and oyster tissues were sampled for biochemical analyses. The survival of oysters exposed to OsHV-1 at pH 7.8 was lower (33.5%) than that of their counterparts at pH 8.1 (44.8%) whereas levels of OsHV-1 DNA were similar. Energetic reserves, fatty acid composition and prostaglandin levels in oyster did not vary...
Tipo: Text
Palavras-chave: Acidification; Bivalve; Disease; Environment; Metabolism; Mortality risk.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00472/58334/60887.pdf

Impact of the oyster Crassostrea gigas on a microbial community in Atlantic coastal ponds near La Rochelle
Provedor de dados: 5
Autores: Dupuy, Christine; Pastoureaud, Annie; Ryckaert, Mireille; Sauriau, Pierre-guy; Montanie, Hélène.
To assess the in situ impact of oysters Crassostrea gigas on planktonic protist and bacteria communities and the potential contribution of protozoa to their food resource intake, the abundance and the diversity of protists and bacteria were followed in 2 Atlantic coastal ponds, with and without oysters. The protist biomass in such ponds was high, with a maximum in spring of 982 mug C l(-1) and a minimum in winter of 179 pg C l(-1). Whatever the season, the presence of oysters (20 m(-2) corresponding to an average of 23 mg dry weight m(-2)) induced a significant decrease in &gt;5 pm protist abundance. On the contrary, planktonic organisms &lt;5 pm, such as Chlorophyta flagellates and bacteria, developed similarly in both ponds. It can be assumed that such...
Tipo: Text
Palavras-chave: Trophic link; Bacteria; Protists; Microbial food web; Coastal pond; Food source; Oysters; Bivalve.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-822.pdf

Derivation of toxicity equivalency factors for marine biotoxins associated with Bivalve Molluscs
Provedor de dados: 5
Autores: Botana, Luis M.; Hess, Philipp; Munday, Rex; Nathalie, Arnich; Degrasse, Stacey L.; Feeley, Mark; Suzuki, Toshiyuki; Van Den Berg, Martin; Fattori, Vittorio; Gamarro, Esther Garrido; Tritscher, Angelika; Nakagawa, Rei; Karunasagar, Iddya.
Background  Seafood toxins pose an important risk to human health, and maximum levels were imposed by regulatory authorities throughout the world. Several toxin groups are known, each one with many analogues of the major toxin. Regulatory limits are set to ensure that commercially available seafood is not contaminated with unsafe levels. Scope and Approach  The mouse bioassay was used to measure the toxicity in seafood extracts to determine if a sample exceeded regulatory limits. The advantage of this approach was to provide an estimation of the total toxicity in the sample. As instrumental methods of analysis advance and serve as replacements to the mouse bioassay, the challenge is translating individual toxin concentrations into toxicity to determine...
Tipo: Text
Palavras-chave: Marine toxins; Toxicity Equivalency Factors; FAO; WHO; Bivalve; Mollusc.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00358/46960/46874.pdf

Growth Response of Arctica Islandica to North Atlantic Oceanographic Conditions Since 1850
Provedor de dados: 5
Autores: Poitevin, Pierre; Thébault, Julien; Siebert, Valentin; Donnet, Sébastien; Archambault, Philippe; Doré, Justine; Chauvaud, Laurent; Lazure, Pascal.
The Northwest Atlantic is a key region with an essential role in global climate regulation, redistributing heat and influencing the carbon cycle. However, little is known about its evolution before 1950, mainly because of the lack of long-term instrumental measurements. The hard parts of long-lived marine biota hold the potential to extend instrumentally derived observation by several decades or centuries and enhance our understanding of global climate processes. Here, we investigate the effects of local, regional, and large-scale climate variability on the marine bivalve, Arctica islandica (Linnaeus, 1767) from Saint-Pierre and Miquelon (SPM). This archipelago lies at the boundary zone between the cold Labrador Current in the north and the warm Gulf...
Tipo: Text
Palavras-chave: Arctica islandica; Paleoecology; North Atlantic; Sub polar gyre; Labrador current; Bivalve; Sclerochronology; Climate change.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00509/62050/66198.pdf

An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas
Provedor de dados: 5
Autores: Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud.
This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,...
Tipo: Text
Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf

Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers
Provedor de dados: 5
Autores: Cavelier, Patricia; Cau, Julien; Morin, Nathalie; Delsert, Claude.
While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster...
Tipo: Text
Palavras-chave: Germline cells; Cell cycle; Reproduction; Bivalve; Marine invertebrates.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00409/52100/52809.pdf

Spatial distribution and nutritional requirements of the endosymbiont-bearing bivalve Loripes lacteus (sensu Poli, 1791) in a Mediterranean Nanozostera noltii (Hornemann) meadow
Provedor de dados: 5
Autores: Rossi, Francesca; Colao, Elodie; Jose Martinez, Maria; Klein, Judith C.; Carcaillet, Frederique; Callier, Myriam; De Wit, Rutger; Caro, Audrey.
Sulphur-oxidising endosymbiont-bearing bivalves often inhabit seagrass meadows, where they can control sulphide levels and variably contribute to carbon cycling, by feeding on endosymbiotic bacteria and/or on particulate organic matter from the water column. The patterns of variability in their feeding mode and their spatial distribution within the seagrass meadows are however poorly studied. Seagrass beds form naturally patchy habitats with seagrass-sand edges that may have variable effects on different organisms. The present study aims at understanding differences in feeding mode and abundance of the endosymbiont-bearing bivalve Loripes lacteus (sensu Poli, 1791) as well as the physiological conditions of its endosymbiotic populations between edge and...
Tipo: Text
Palavras-chave: Bivalve; Food web; Mediterranean; Seagrass; Stable isotopes; Symbiosis.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00138/24911/26373.pdf

Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane.
To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to...
Tipo: Text
Palavras-chave: Ingestion; Gene expression; Bivalve; Alpha amylase.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-5165.pdf

Metabolism of the Pacific oyster,     Crassostrea gigas     , is influenced by salinity and modulates survival to the Ostreid herpesvirus OsHV-1
Provedor de dados: 5
Autores: Fuhrmann, Marine; Delisle, Lizenn; Petton, Bruno; Corporeau, Charlotte; Pernet, Fabrice.
The Pacific oyster, Crassostrea gigas, is an osmoconforming bivalve exposed to wide salinity fluctuations. The physiological mechanisms used by oysters to cope with salinity stress are energy demanding and may impair other processes, such as defense against pathogens. This oyster species has been experiencing recurrent mortality events caused by the Ostreid herpesvirus 1 (OsHV-1). The objectives of this study were to investigate the effect of salinity (10, 15, 25 and 35‰) on energetic reserves, key enzyme activities and membrane fatty acids, and to identify the metabolic risk factors related to OsHV-1-induced mortality of oysters. Acclimation to low salinity led to increased water content, protein level, and energetic reserves (carbohydrates and...
Tipo: Text
Palavras-chave: Bivalve; Disease; Environment; Metabolism; Mortality risk; Salinity.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00427/53886/54958.pdf

Influence du mode de stockage sur la toxicité d'une eau et d'un sédiment à l'aide du bio-essai embryon de bivalve - Etude préliminaire
Provedor de dados: 5
Autores: Damee, Nicolas.
La conservation des échantillons a souvent constitué un problème tant pour les chimistes que pour les biologistes. Si les chimistes semblent satisfaits de leurs techniques de conservation, les biologistes quant à eux sont indécis du fait que l'action toxique se modifie au cours du temps et selon la technique employée (réfrigération, congélation, lyophilisation, conservation en azote liquide à -196°C). Le présent rapport constitue une étude préliminaire qui, si elle est poursuivie, pourrait permettre de déterminer le mode de conservation le plus approprié pour chaque type d'échantillons.
Tipo: Text
Palavras-chave: Conservation; Bio-essai; Pourcentage net d'anomalies (PNA); Sédiment; Bivalve.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00037/14828/12144.pdf

French scallops: A new host for ostreid herpesvirus-1
Provedor de dados: 5
Autores: Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan.
Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been...
Tipo: Text
Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf

First evidence of a potential antibacterial activity involving a laccase-type enzyme of the phenoloxidase system in Pacific oyster Crassostrea gigas haemocytes
Provedor de dados: 5
Autores: Luna-acosta, Andrea; Saulnier, Denis; Pommier, Mylene; Haffner, Philippe; De Decker, Sophie; Renault, Tristan; Thomas-guyon, Helene.
Phenoloxidases (POs) are a group of copper proteins including tyrosinase, catecholase and laccase. In several insects and crustaceans, antibacterial substances are produced through the PO cascade, participating in the direct killing of invading microorganisms. However, although POs are widely recognised as an integral part of the invertebrate immune defence system, experimental evidence is lacking that these properties are conserved in molluscs, and more particularly in the Pacific oyster Crassostrea gigas. In the present study, Vibrio splendidus LGP32 and Vibrio aestuarianus 02/041 growths were affected, after being treated with C. gigas haemocyte lysate supernatant (HLS), and either a common substrate of POs, l-3,4-dihydroxyphenylalanine (l-DOPA), to...
Tipo: Text
Palavras-chave: Phenoloxidases; Antibacterial activity; Vibrio; Bivalve; Immunity.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00045/15598/13001.pdf

Effect of a mono-specific algal diet on immune functions in two bivalve species - Crassostrea gigas and Ruditapes philippinarum
Provedor de dados: 5
Autores: Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois.
The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte...
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf

Effects of marine harmful algal blooms on bivalve cellular immunity and infectious diseases: A review
Provedor de dados: 5
Autores: Lassudrie Duchesne, Malwenn; Hegaret, Helene; Wikfors, Gary H; Mirella Da Silva, Patricia.
Bivalves were long thought to be “symptomless carriers” of marine microalgal toxins to human seafood consumers. In the past three decades, science has come to recognize that harmful algae and their toxins can be harmful to grazers, including bivalves. Indeed, studies have shown conclusively that some microalgal toxins function as active grazing deterrents. When responding to marine Harmful Algal Bloom (HAB) events, bivalves can reject toxic cells to minimize toxin and bioactive extracellular compound (BEC) exposure, or ingest and digest cells, incorporating nutritional components and toxins. Several studies have reported modulation of bivalve hemocyte variables in response to HAB exposure. Hemocytes are specialized cells involved in many functions in...
Tipo: Text
Palavras-chave: Bivalve; Harmful algal blooms (HABs); Pathogen; Hemocyte; Disease.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00612/72401/71346.pdf

Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum
Provedor de dados: 5
Autores: Paillard, Christine; Gausson, Sabrina; Nicolas, Jean-louis; Le Pennec, Jean Paul; Haras, Dominique.
Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V...
Tipo: Text
Palavras-chave: DOT BLOT hybridisation; SSP PCR; Vibriosis; Bivalve; Detection; Diagnostic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1692.pdf

Molecular cloning and seasonal expression of oyster glycogen phosphorylase and glycogen synthase genes
Provedor de dados: 5
Autores: Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne.
To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes...
Tipo: Text
Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf

Contrôle de la ploidie par imagerie numérique dans des expériences d'induction de la triploidie chez les mollusques bivalves
Provedor de dados: 5
Autores: Gerard, Andre; Peignon, Jean-marie; Chagot, Dominique.
Individual determination of ploidy level in experimentally induced triploid bivalve molluscs using image cytometry. The sexual maturation in bivalve molluscs causes decreased growth and sometimes increased mortalities. These drawbacks can be avoided by genetic sterilization (triploidy). Triploïdy induction require a egg treatment and a ploidy control at each generation. The ploidy level is usually determined by counting the chromosomes, a technique which is relatively easy for the trochophora stage and for juveniles measuring from 5 mm to 3 cm only.  ln order to be able to follow up the evolution of the ploidy rate during any stage of animallife, we have developed an estimation method based on image cytometry. Individual DNA content is determined by...
Tipo: Text
Palavras-chave: Image cytometry; Caryology; Triploidy; Bivalve; Imagerie numérique; Caryologie; Triploïdie; Bivalve.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-3202.pdf

Temperature and seston quantity and quality effects on field reproduction of farmed oysters, Crassostrea gigas, in Bourgneuf Bay, France
Provedor de dados: 5
Autores: Dutertre, Mickael; Beninger, Peter G.; Barille, Laurent; Papin, Mathias; Rosa, Philippe; Barille, Anne-laure; Haure, Joel.
The proliferation of the voluntarily-introduced cupped oyster, Crassostrea gigas, has attained the proportions of species invasion in many intertidal habitats in Europe, presumably resulting from successful reproduction of farmed individuals. It is thus imperative to better understand the reproductive characteristics of farmed oysters, since they are directly under human control. We quantified the dry tissue mass (DTM), gametosomatic index (GSI), and reproductive cycle of farmed oysters at two sites in Bourgneuf Bay, France, in relation to environmental parameters using continuously-recording probes in 2005 and 2006. The GSI was developed for this study, based on the actual area occupied by gametes, rather than the area of the gonad previously used for...
Tipo: Text
Palavras-chave: Atlantic Ocean; Crassostrea gigas; Oyster farming; Bivalve; Gametosomatic index; Biological invasion; Atresia; Oocytes; Turbidity; Temperature; Reproduction.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6871.pdf

Marine diatoms sustain growth of bivalves in a Mediterranean lagoon
Provedor de dados: 5
Autores: Pernet, Fabrice; Malet, Nathalie; Pastoureaud, Annie; Vaquer, Andre; Quere, Claudie; Dubroca, Laurent.
Carbon stable isotopes and fatty acids were measured in the suspended particulate organic matter (POM) of the Thau lagoon to study its qualitative temporal changes in relation to environmental factors and to identify the food sources of bivalves over a one-yr-cycle in relation to their growth. Reciprocally, the impact of shellfish farming on POM was also studied. Oysters and mussels were sampled and measured for biometry, stable isotopes and fatty acid composition. Water samples were collected at two sites, both inside and outside of the shellfish farming area, to determine concentrations in POM, chlorophyll a (Chl a) and stable isotopes. Carbon isotopes and fatty acids in bivalves reflected seasonal changes in food sources, which varied consistently with...
Tipo: Text
Palavras-chave: Aquaculture; Bivalve; Competition; Feeding ecology; Growth; Trophic markers.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00075/18575/16177.pdf

Impact of 20 : 4n-6 supplementation on the fatty acid composition and hemocyte parameters of the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Giudicelli, Emilie; Lambert, Christophe; Seguineau, Catherine; Samain, Jean-francois.
Arachidonic acid (20:4n-6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 mu g ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by...
Tipo: Text
Palavras-chave: Hemocyte parameters; Oyster immunity; Arachidonic Acid; Poly unsaturated fatty acids; Nutrition; Bivalve; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1876.pdf

Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf

Hermaphroditism in Marine Mussel Perumytilus purpuratus (Lamarck, 1819), (Mollusca: Mytilidae)
Provedor de dados: 77
Autores: Montenegro Villalobos,Diana; Olivares Paz,Alberto; González,María Teresa.
Marine bivalves are essentially gonochoric species. However, some occasional hermaphrodites specimens can be found. A histological description of two (among 179) hermaphrodites of the marine mussel Perumytilus purpuratus (Lamarck, 1819) that were collected in San Jorge Bay (24S) is given. The low proportion of hermaphrodites suggests that P. purpuratus hermaphroditism could be an accidental phenomenon; nevertheless other causes are also discussed in the present study.
Tipo: Journal article
Palavras-chave: Bivalve; Hermaphroditism; Perumytilus purpuratus.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200038

Gonadal development and sexuality of Larkinia grandis (Arcida: Arcidae) inhabiting southeastern Gulf of California
Provedor de dados: 103
Autores: Sotelo-Gonzalez,Maria I.; García-Ulloa,Manuel; Góngora-Gómez,Andrés M.; Salcido-Guevara,Luis A.; Arzola-González,Juan F.; Sepúlveda,Carlos H.; Sánchez-Cárdenas,Rebeca.
ABSTRACT Larkinia grandis (Broderip &amp; G.B. Sowerby I, 1829), an important fishing resource for Mexican communities, is an Arcidae clam. It is also considered a species with aquaculture potential. In this work we investigated the gonadal phases and sexuality in a population of L. grandis in the Gulf of California. Our findings support the hypothesis that there is one male per female in the population studied. It also documents that the shape, position and color of the gonads of L. grandis are consistent with observations in other Arcidae species. Additionally, five gonadal phases are differentiated and described in males and females (development, mature, spawning, post-spawning and resting), with a noticeable presence of brown cells during post-spawning...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bivalve; Estuary; Gametogenesis; Gonad; Mangrove cockle; Sex cells.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702021000100306

Monitoramento histopatológico de mexilhão Perna perna da Lagoa de Itaipu, Niterói, RJ
Provedor de dados: 45
Autores: Lima,F.C.; Abreu,M.G.; Mesquita,E.F.M.
Cortes histológicos de 120 mexilhões Perna perna (Linnaeus, 1758), coletados na Lagoa de Itaipu, Niterói, RJ, por um período de 12 meses, foram examinados quanto a presença de parasitas e lesões. Noventa exemplares apresentaram cinco diferentes processos patológicos: bucefalose, presença de protozoários Nematopsis sp., inclusões basofílicas não específicas, encapsulamentos parasitários e cistos epiteliais branquiais. Cinquenta animais mostravam duas ou mais patologias concomitantes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mexilhão; Perna perna; Bivalve; Parasita; Histopatologia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000200013

Recolonization of experimental gaps by the mussels Brachidontes darwinianus and B. solisianus in a subtropical rocky shore
Provedor de dados: 52
Autores: Tanaka,Marcel Okamoto.
In this study, the recolonization speed of the mussels Brachidontes darwinianus and B. solisianus was compared to evaluate their responses to gaps opened by disturbances in the rocky shore. B. darwinianus was much faster than B. solisianus, rapidly closing gaps in the mussel bed. When they co-occurred, distribution patterns of these species were related not only with distinct physiological tolerances but also with responses to disturbance. B. darwinianus was more tolerant to low salinities, dominated estuarine zones generally occuring lower in the midlittoral, and presented a faster response to opened gaps.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bivalve; Intertidal; Rocky shore; Zonation; Mytilidae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100015