Consulta: subjectFacets:"Mitochondrial DNA"
Registros recuperados: 68
Data/hora: 09/06/2024 10:48:51

Identificación molecular de heces y análisis de hábitos alimenticios de carnívoros en la reserva de la biósfera "Sierra del Abra Tanchipa", San Luis Potosí, México.
Provedor de dados: 32
Autores: Benítez Alemán, Héctor Eduardo.
La identificación precisa de heces de carnívoros silvestres es  importante para describir sus hábitos alimenticios, sobre todo en aquellas especies elusivas o en riesgo. Sin embargo Identificar las heces de especies de carnívoros simpátricos no es una tarea sencilla. Por ello, las técnicas moleculares, son herramientas que brindan mayor precisión en la identificación de heces. El análisis de hábitos alimenticios aporta información valiosa para entender la estructura poblacional de los depredadores y sus presas, así como su importancia ecológica para generar estrategias de manejo y conservación de carnívoros y sus presas. Los objetivos de este estudio fueron identificar las heces de carnívoros extrayendo ADN mitocondrial de estas y amplificando un fragmento...
Palavras-chave: ADN mitocrondrial; Citocromo b; PCR-RFLP; Hábitos alimenticios; Carnívoros; Mitochondrial DNA; Cytochrome b; Food habits; Carnivores; Ganadería; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2291

Mitochondrial DNA common deletion is not associated with thyroid, breast and colorectal tumors in Turkish patients
Provedor de dados: 74
Autores: Aral,Cenk; Akkiprik,Mustafa; Kaya,Handan; Ataizi-Çelikel,Çigdem; Çaglayan,Sinan; Özisik,Gökhan; Baloglu,Hüseyin; Özer,Ayse.
Recently, efforts have been focused on mitochondrial DNA changes and their relation to human cancers. Among them, a 4977 bp deletion of mitochondrial DNA, named "common deletion", has been investigated in several types of tumors, with inconsistent results. In this study, we investigated the presence of the common deletion in tissues from 25 breast, 25 colorectal and 50 thyroid tumors and in the adjacent healthy tissues from Turkish patients. Samples from healthy volunteers were also evaluated for comparison. Two PCR-based methods were used for the detection of the common deletion. First, two pairs of primers were used to amplify wild-type and deleted mtDNA. Then, a highly sensitive nested-PCR was performed, to determine low amounts of deleted genomes. By...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Common deletion; Cancer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100001

Demographic history of wood stork (Mycteria americana) Brazilian Pantanal colonies revealed by mitochondrial DNA analysis
Provedor de dados: 74
Autores: Lopes,Iara Freitas; Brito,Reinaldo Alves de; Henrique-Silva,Flávio; Del Lama,Silvia Nassif.
We used mitochondrial DNA (mtDNA) sequences to investigate the demographic history of the wood stork (Mycteria americana) populations in the Brazilian Pantanal. Sequences of 390/460 bp fragment of the mtDNA control region were analyzed in 62 wood stork specimens from 8 colonies using neutrality tests, phylogeographic, and coalescent analyses. Population expansion was supported by the significantly negative values of Tajima’s (D = -2.071) and Fu’s (Fs = -14.544) statistics and the unimodal pattern of mismatch distribution. Nested clade analyses indicated a historic range expansion event and recurrent gene flow that was restricted by isolation by distance as explanations for the haplotype distribution among the sampled colonies. High genetic diversity and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wood stork; Mycteria americana; Pantanal; Mitochondrial DNA; Phylogeography.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200008

Molecular evidence for a recent demographic expansion in the puma (Puma concolor) (Mammalia, Felidae)
Provedor de dados: 74
Autores: Matte,Eunice M.; Castilho,Camila S.; Miotto,Renata A.; Sana,Denis A.; Johnson,Warren E.; O'Brien,Stephen J.; Freitas,Thales R. O. de; Eizirik,Eduardo.
The puma is an iconic predator that ranges throughout the Americas, occupying diverse habitats. Previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. Moreover, most of the species' molecular diversity was found to be in South America. To further investigate the phylogeographic structure and demographic history of pumas we analyzed mtDNA sequences from 186 individuals sampled throughout their range, with emphasis on South America. Our objectives were to refine the phylogeographic assessment within South America and to investigate the demographic history of pumas using a coalescent approach. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Mammals; Mitochondrial DNA; Molecular time estimate; Phylogeography.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400018

Phylogenetic analysis of the order Pleuronectiformes (Teleostei) based on sequences of 12S and 16S mitochondrial genes
Provedor de dados: 74
Autores: Azevedo,Marisa F.C.; Oliveira,Claudio; Pardo,Belén G.; Martínez,Paulino; Foresti,Fausto.
The fish order Pleuronectiformes, composed of 14 families, has two suborders: Psettodoidei (with one family) and Pleuronectoidei (with thirteen families). The relationships among families of Pleuronectoidei and among the genera of their families have extensively been debated and a consensus has not yet been reached. In the present study, partial sequences of the 12S and 16S mitochondrial rRNA genes were obtained from 19 species belonging to the families Achiridae, Bothidae, Cynoglossidae, Paralichthyidae, Pleuronectidae, Scophthalmidae, and Soleidae. Additional sequences of 42 pleuronectiform species were obtained from GenBank. Phylogenetic analyses were conducted by the methods of maximum-parsimony, maximum-likelihood and Bayesian inference. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Flatfishes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200023

A phylogenetic analysis of Brycon and Henochilus (Characiformes, Characidae, Bryconinae) based on the mitochondrial gene 16S rRNA
Provedor de dados: 74
Autores: Hilsdorf,Silva; Oliveira,Claudio; Lima,Flávio César Thadeo de; Matsumoto,Cristianne Kayoko.
The genus Brycon, the largest subunit of the Bryconinae, has 42 valid species distributed from southern Mexico to the La Plata River in Argentina. Henochilus is a monotypic genus, comprising a single species (H. wheatlandii) found in the upper Rio Doce basin. In the present study, partial sequences of the mitochondrial gene 16S were obtained for fifteen species of Brycon and for Henochilus wheatlandii. The results showed that the genus Brycon is paraphyletic, since Henochilus is the sister-group of B. ferox and B. insignis. The most basal species analyzed were the trans-Andean species B. henni, B. petrosus, and B. chagrensis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Systematic.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200034

Low levels of genetic diversity depicted from mitochondrial DNA sequences in a heavily exploited marine fish (Cynoscion acoupa, Sciaenidae) from the Northern coast of Brazil
Provedor de dados: 74
Autores: Rodrigues,Rosa; Schneider,Horacio; Santos,Simôni; Vallinoto,Marcelo; Sain-Paul,Ulrich; Sampaio,Iracilda.
The acoupa weakfish (Cynoscion acoupa - Sciaenidae) is a marine species of croaker with estuarine-dependent behavior, found in the western Atlantic from Panama to Argentina. It is one of the most exploited food fish on the northern coast of Brazil. In this study, DNA sequences were determined from the entire control region (D-loop) of the mitochondrial genome of 297 individuals collected during seven different months between December 2003 and August 2005 on the northern coast of Brazil (Amapá and Pará). Genetic variability expressed by haplotype (h = 0,892) and nucleotide (pi = 0,003) diversities were low compared to other heavily exploited marine fish species from the western Atlantic and eastern Asia. AMOVA depicted a lack of genetic structuring among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cynoscion acoupa; Acoupa weakfish; Sciaenidae; Low genetic diversity; Mitochondrial DNA; D-loop.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300015

Variation in the mitochondrial control region sequence between the two maternal lines of the Sorraia horse breed
Provedor de dados: 74
Autores: Luís,Cristina; Bastos-Silveira,Cristiane; Cothran,E.Gus; Oom,M. Mar.
The mitochondrial control region sequence was compared between the two surviving matrilineal lineages of the Sorraia horse. Two haplotypes were found, showing three differences between them. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorraia horse; Mitochondrial DNA; Maternal lineages; Control region.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300010

Phylogenetic analysis of 16S mitochondrial DNA data in sloths and anteaters
Provedor de dados: 74
Autores: Barros,Maria Claudene; Sampaio,Iracilda; Schneider,Horacio.
We sequenced part of the 16S rRNA mitochondrial gene in 17 extant taxa of Pilosa (sloths and anteaters) and used these sequences along with GenBank sequences of both extant and extinct sloths to perform phylogenetic analysis based on parsimony, maximum-likelihood and Bayesian methods. By increasing the taxa density for anteaters and sloths we were able to clarify some points of the Pilosa phylogenetic tree. Our mitochondrial 16S results show Bradypodidae as a monophyletic and robustly supported clade in all the analysis. However, the Pleistocene fossil Mylodon darwinii does not group significantly to either Bradypodidae or Megalonychidae which indicates that trichotomy best represents the relationship between the families Mylodontidae, Bradypodidae and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pilosa; Sloths; Anteaters; Phylogeny; Mitochondrial DNA; RDNA16S.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100002

Inclusion of South American samples reveals new population structuring of the blacktip shark (Carcharhinus limbatus) in the western Atlantic
Provedor de dados: 74
Autores: Sodré,Davidson; Rodrigues-Filho,Luis F.S.; Souza,Rosália F.C.; Rêgo,Péricles S.; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
Carcharhinus limbatus has a cosmopolitan distribution and marked genetic structuring, mainly because of its philopatric behavior. However, analysis of this structuring has not previously included South American populations. In the present study, we analyzed a sample of adult individuals collected on the northern coast of Brazil and compared the sequences of the mitochondrial control region with those of populations already genotyped. Relatively high haplotype diversity (12 haplotypes, genetic diversity of 0.796) was observed, similar to that in other populations but with a much larger number of private alleles. In contrast to populations studied previously, which were represented by neonates, the pronounced allelic variability found in the South American...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcharhinus limbatus; Control region; Fishery stocks; Mitochondrial DNA; Population genetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500008

Reconstruction of major maternal and paternal lineages of the Cape Muslim population
Provedor de dados: 74
Autores: Isaacs,Shafieka; Geduld-Ullah,Tasneem; Benjeddou,Mongi.
The earliest Cape Muslims were brought to the Cape (Cape Town -South Africa) from Africa and Asia from 1652 to 1834. They were part of an involuntary migration of slaves, political prisoners and convicts, and they contributed to the ethnic diversity of the present Cape Muslim population of South Africa. The history of the Cape Muslims has been well documented and researched however no in-depth genetic studies have been undertaken. The aim of the present study was to determine the respective African, Asian and European contributions to the mtDNA (maternal) and Y-chromosomal (paternal) gene pool of the Cape Muslim population, by analyzing DNA samples of 100 unrelated Muslim males born in the Cape Metropolitan area. A panel of six mtDNA and eight Y-chromosome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR-RFLP; Genetic polymorphism; Mitochondrial DNA; Population genetic structure; Chromosome variations.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200005

Mitochondrial DNA phylogeography of Brazilian populations of Drosophila buzzatii
Provedor de dados: 74
Autores: Brito,Reinaldo A. de; Manfrin,Maura H.; Sene,Fabio M.
The Argentinean Chaco region has been considered the center of origin of Drosophila buzzatii in South America because it contains most of the chromosomal polymorphism detected in natural populations. Two hypotheses have been put forward to explain the distribution of D. buzzatii in Brazil, one proposing that it has only recently passively colonized Brazil via human dispersal and the other suggesting that D. buzzatii has actively migrated to Brazil some time ago. Data from chromosomal inversions support recent colonization, whereas data from allozymes and mtDNA variation indicate that D. buzzatii has been in Brazil longer, favoring an active dispersal hypothesis. In our present work we analyzed data on 56 South American flies, mostly from Brazil, sequenced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila buzzatii; Population structure; Mitochondrial DNA; Phylogeography; Brazil; Climatic cycles.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200009

Challenges and prospects of population genetic studies in terns (Charadriiformes, Aves)
Provedor de dados: 74
Autores: Faria,Patrícia J.; Baus,Erika; Morgante,João S.; Bruford,Michael W.
Little information is available about the population structure of communally nesting terns (Sternidae) and skimmers (Rynchopidae) throughout the world. In order to fill this gap, a survey of molecular markers was carried out for six species of terns (Anous stolidus, Sterna hirundinacea, S. fuscata, S. superciliaris, Thalasseus maximus and Phaetusa simplex) and one species of skimmer (Rynchops niger). First, we describe the results of the construction of genomic DNA libraries and document problems encountered during this procedure. Secondly, we tested the cross-amplification of 18 microsatellite loci previously described for related species (the number of polymorphic loci ranged from three to seven). Thirdly, we tested the usefulness of mtDNA (control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Microsatellites; Mitochondrial DNA; Terns.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400029

A recombination point is conserved in the mitochondrial genome of higher plant species and located downstream from the cox2 pseudogene in Solanum tuberosum L.
Provedor de dados: 74
Autores: Tada,Susely F.S.; Souza,Anete P.
The potato (Solanum tuberosum L.) mitochondrial cox3/sdh4/pseudo-cox2 gene cluster has previously been identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In our present study we used Southern blotting using sdh4 and cox2 probes to show that the sdh4 and cox2 genes are clustered in the mitochondria of potato, soybean and pea. Northern blotting revealed cotranscription of sdh4 and cox2 in potato but not in cauliflower, indicating that these genes are not clustered in cauliflower. A putative recombination point was detected downstream of the cox2 pseudogene (pseudo-cox2) in potato mitochondrial DNA (mtDNA). This sequence corresponds to a 32 bp sequence which appears to be well-conserved and is adjacent to the terminals of some...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Rearrangement; Plants; Solanum tuberosum; Cox2.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100017

Molecular evidence of two cryptic species of Stramonita (Mollusca, Muricidae) in the southeastern Atlantic coast of Brazil
Provedor de dados: 74
Autores: De Biasi,Juliana Beltramin; Tomás,Acácio Ribeiro Gomes; Hilsdorf,Alexandre Wagner Silva.
Abstract Snails of the genus Stramonita are commonly found in the rocky intertidal habitat of the western Atlantic Ocean coast. They belong to a monophyletic taxon that occurs along the tropical and warm-temperate Atlantic and eastern Pacific rocky shores. This genus comprises different valid species and members of the S. haemastoma complex. In the present study, samples of Stramonita were collected from three different regions of southeastern Brazil. Partial sequences of two mitochondrial genes, COI and 16S rRNA, were used to compare nucleotides sequences between Stramonita specimens. Levels of nucleotide divergence greater than 2% across the three sampled regions were used for differentiation at the species level. One of the identified species was S....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: COI; 16s rRNA; Mitochondrial DNA; Southern oyster drill; Brazilian coast.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300392

D-loop haplotype diversity in Brazilian horse breeds
Provedor de dados: 74
Autores: Ianella,Patrícia; Albuquerque,Maria do Socorro Maués; Paiva,Samuel Rezende; Egito,Andréa Alves do; Almeida,Leonardo Daniel; Sereno,Fabiana T. P. S.; Carvalho,Luiz Felipe Ramos; Mariante,Arthur da Silva; McManus,Concepta Margaret.
Abstract The first horses were brought to Brazil by the colonizers after 1534. Over the centuries, these animals evolved and adapted to local environmental conditions usually unsuitable for exotic breeds, thereby originating locally adapted Brazilian breeds. The present work represents the first description of maternal genetic diversity in these horse breeds based on D-loop sequences. A D-Loop HSV-I fragment of 252 bp, from 141 horses belonging to ten Brazilian breeds / genetic groups (locally adapted and specialized breeds) were analysed. Thirty-five different haplotypes belonging to 18 haplogroups were identified with 33 polymorphic sites. Haplotype diversity (varying from 0.20 to 0.96) and nucleotide diversity (varying from 0.0039 to 0.0239) was lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Genetic characterization; Equus caballus; Locally adapted breeds; Animal genetic resources.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400604

Genetic differences between Chibcha and Non-Chibcha speaking tribes based on mitochondrial DNA (mtDNA) haplogroups from 21 Amerindian tribes from Colombia
Provedor de dados: 74
Autores: Usme-Romero,Solangy; Alonso,Milena; Hernandez-Cuervo,Helena; Yunis,Emilio J.; Yunis,Juan J.
We analyzed the frequency of four mitochondrial DNA haplogroups in 424 individuals from 21 Colombian Amerindian tribes. Our results showed a high degree of mtDNA diversity and genetic heterogeneity. Frequencies of mtDNA haplogroups A and C were high in the majority of populations studied. The distribution of these four mtDNA haplogroups from Amerindian populations was different in the northern region of the country compared to those in the south. Haplogroup A was more frequently found among Amerindian tribes in northern Colombia, while haplogroup D was more frequent among tribes in the south. Haplogroups A, C and D have clinal tendencies in Colombia and South America in general. Populations belonging to the Chibcha linguistic family of Colombia and other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Amerindian; Colombia; Chibcha; Genetic relationships.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200003

Genetic frequencies related to severe or profound sensorineural hearing loss in Inner Mongolia Autonomous Region
Provedor de dados: 74
Autores: Liu,Yongzhi; Ao,Liying; Ding,Haitao; Zhang,Dongli.
Abstract The aim was to study the frequencies of common deafness-related mutations and their contribution to hearing loss in different regions of Inner Mongolia. A total of 738 deaf children were recruited from five different ethnic groups of Inner Mongolia, including Han Chinese (n=486), Mongolian (n=216), Manchurian (n=24), Hui (n=6) and Daur (n=6). Nine common mutations in four genes (GJB2, SLC26A4, GJB3 and mitochondrial MT-RNR1 gene) were detected by allele-specific PCR and universal array. At least one mutated allele was detected in 282 patients. Pathogenic mutations were detected in 168 patients: 114 were homozygotes and 54 were compound heterozygotes. The 114 patients were carriers of only one mutated allele. The frequency of GJB2 variants in Han...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sensorineural hearing loss; GJB2; SLC26A4; GJB3; Mitochondrial DNA; Inner Mongolia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400567

Simultaneous quantitative assessment of circulating cell-free mitochondrial and nuclear DNA by multiplex real-time PCR
Provedor de dados: 74
Autores: Xia,Peng; Radpour,Ramin; Zachariah,Rebecca; Fan,Alex Xiu Cheng; Kohler,Corina; Hahn,Sinuhe; Holzgreve,Wolfgang; Zhong,Xiao Yan.
Quantification of circulating nucleic acids in plasma and serum could be used as a non-invasive diagnostic tool for monitoring a wide variety of diseases and conditions. We describe here a rapid, simple and accurate multiplex real-time PCR method for direct synchronized analysis of circulating cell-free (ccf) mitochondrial (mtDNA) and nuclear (nDNA) DNA in plasma and serum samples. The method is based on one-step multiplex real-time PCR using a FAM-labeled MGB probe and primers to amplify the mtDNA sequence of the ATP 8 gene, and a VIC-labeled MGB probe and primers to amplify the nDNA sequence of the glycerinaldehyde-3-phosphate-dehydrogenase (GAPDH) gene, in plasma and serum samples simultaneously. The efficiencies of the multiplex assays were measured in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circulating cell-free DNA; Mitochondrial DNA; Nuclear DNA; Real-time PCR; Quantitative PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100003

Uniparental genetic markers in South Amerindians
Provedor de dados: 74
Autores: Bisso-Machado,Rafael; Bortolini,Maria Cátira; Salzano,Francisco Mauro.
A comprehensive review of uniparental systems in South Amerindians was undertaken. Variability in the Y-chromosome haplogroups were assessed in 68 populations and 1,814 individuals whereas that of Y-STR markers was assessed in 29 populations and 590 subjects. Variability in the mitochondrial DNA (mtDNA) haplogroup was examined in 108 populations and 6,697 persons, and sequencing studies used either the complete mtDNA genome or the highly variable segments 1 and 2. The diversity of the markers made it difficult to establish a general picture of Y-chromosome variability in the populations studied. However, haplogroup Q1a3a* was almost always the most prevalent whereas Q1a3* occurred equally in all regions, which suggested its prevalence among the early...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetics; Language and geography; Mitochondrial DNA; Native Americans; South Amerindians; Y-chromosome.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300001

Sciaenidae fish of the Caeté River estuary, Northern Brazil: mitochondrial DNA suggests explosive radiation for the Western Atlantic assemblage
Provedor de dados: 74
Autores: Vinson,Christina; Gomes,Grazielle; Schneider,Horacio; Sampaio,Iracilda.
Sciaenids are fish which are normally abundant in tropical estuaries of the western Atlantic. Studies on the Caeté river estuary in the northern Brazilian state of Pará have revealed that in this area Sciaenidae is the dominant family, comprising almost 50% of all teleosts sampled. In this paper we present the results of the first phylogenetic study on South American estuarine sciaenids, during which we obtained mitochondrial gene 16S sequences from 15 species belonging to eight genera occurring in the Caeté estuary. Intergeneric nucleotide divergences varied from 5 to 15%, Lonchurus and Menticirrhus being the most divergent lineages. Nucleotide divergences were quite variable amongst species of the same genus, ranging from 1.2% (Stellifer microps x...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sciaenidae; Perciformes; Mitochondrial DNA; RRNA 16S; Caeté River estuary.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200008

Colombian Creole horse breeds: same origin but different diversity
Provedor de dados: 74
Autores: Jimenez,Ligia Mercedes; Mendez,Susy; Dunner,Susana; Cañón,Javier; Cortés,Óscar.
In order to understand the genetic ancestry and mitochondrial DNA (mtDNA) diversity of current Colombian horse breeds we sequenced a 364-bp fragment of the mitocondrial DNA D-loop in 116 animals belonging to five Spanish horse breeds and the Colombian Paso Fino and Colombian Creole cattle horse breeds. Among Colombian horse breeds, haplogroup D had the highest frequency (53%), followed by haplogroups A (19%), C (8%) and F (6%). The higher frequency of haplogroup D in Colombian horse breeds supports the theory of an ancestral Iberian origin for these breeds. These results also indicate that different selective pressures among the Colombian breeds could explain the relatively higher genetic diversity found in the Colombian Creole cattle horse when compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colombian; Creole cattle horse; Horse breeds; Mitochondrial DNA; Paso Fino horse breed.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500013

Identification of Astyanax altiparanae (Teleostei, Characidae) in the Iguaçu River, Brazil, based on mitochondrial DNA and RAPD markers
Provedor de dados: 74
Autores: Prioli,Sônia M.A.P.; Prioli,Alberto J.; Júlio Jr.,Horácio F.; Pavanelli,Carla S.; Oliveira,Alessandra V. de; Carrer,Helaine; Carraro,Dirce M.; Prioli,Laudenir M.
Astyanax fishes are among the most important food-web components of South America rivers. In the Iguaçu River basin, the Astyanax genus is represented mainly by endemic species. For millions of years, that hydrographic basin has been geographically isolated from the Paraná River basin by the Iguaçu Falls. Recently, a species from the Upper Paraná River basin identified as Astyanax bimaculatus was revised and described as a new species named Astyanax altiparanae Garutti &amp;amp; Britski, 2000. Fauna endemism and geographic isolation triggered interest in investigations to evaluate the identification and genetic relatedness among two A. altiparanae populations from the Upper Paraná River basin and the population identified as A. bimaculatus in the Iguaçu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax; Fish; D-loop; Control region; Mitochondrial DNA; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400011

Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Provedor de dados: 74
Autores: da Silva Santos,Anelisie; Trigo,Tatiane Campos; de Oliveira,Tadeu Gomes; Silveira,Leandro; Eizirik,Eduardo.
Abstract The pampas cat is a small felid that occurs in open habitats throughout much of South America. Previous studies have revealed intriguing patterns of morphological differentiation and genetic structure among its populations, as well as molecular evidence for hybridization with the closely related L. tigrinus. Here we report phylogeographic analyses encompassing most of its distribution (focusing particularly on Brazilian specimens, which had been poorly sampled in previous studies), using a novel dataset comprising 2,143 bp of the mitogenome, along with previously reported mtDNA sequences. Our data revealed strong population strutucture and supported a west-to-east colonization process in this species’ history. We detected two population expansion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeography; Population genetics; Mitochondrial DNA; Conservation genetics; Historical demography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200273

Mitochondrial DNA (mtDNA) haplogroups in 1526 unrelated individuals from 11 Departments of Colombia
Provedor de dados: 74
Autores: Yunis,Juan J.; Yunis,Emilio J.
The frequencies of four mitochondrial Native American DNA haplogroups were determined in 1526 unrelated individuals from 11 Departments of Colombia and compared to the frequencies previously obtained for Amerindian and Afro-Colombian populations. Amerindian mtDNA haplogroups ranged from 74% to 97%. The lowest frequencies were found in Departments on the Caribbean coast and in the Pacific region, where the frequency of Afro-Colombians is higher, while the highest mtDNA Amerindian haplogroup frequencies were found in Departments that historically have a strong Amerindian heritage. Interestingly, all four mtDNA haplogroups were found in all Departments, in contrast to the complete absence of haplogroup D and high frequencies of haplogroup A in Amerindian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Admixture; Colombia; Haplogroup; Mestizo; Mitochondrial DNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300005

Phylogeographic history of South American populations of the silky anteater Cyclopes didactylus (Pilosa: Cyclopedidae)
Provedor de dados: 74
Autores: Coimbra,Raphael Teodoro Franciscani; Miranda,Flávia Regina; Lara,Camila Clozato; Schetino,Marco Antônio Alves; Santos,Fabrício Rodrigues dos.
Abstract Cyclopes didactylus, commonly called silky anteater, is the smallest and least studied of the anteaters. It is an arboreal species occurring in rainforests, ranging from southern Mexico to Central and South America, with an apparently disjoint distribution between Amazon and Atlantic rainforests in Brazil. Although seven subspecies are recognized, little is known about its geographical variation. Thus, to evaluate the population dynamics and evolutionary history of the South American silky anteater, we analyzed 1542 bp sequences of the mitochondrial control region (CR), COI and Cyt-b genes of 32 individuals. Haplotype network, AMOVA and molecular dating analyses were performed and identified seven geographic clusters. The split of lineages...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xenarthra; Mitochondrial DNA; Population structure; Molecular dating; South America.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100040

Diversité et différentiation génétiques des populations de tortues vertes (Chelonia mydas) dans les sites de ponte et d'alimentation du sud-ouest de l'océan Indien : application aux stratégies de conservation de l'espèce
Provedor de dados: 5
Autores: Taquet, Coralie.
The green turtle (Chelonia mydas) is an emblematic species of marine life. However, nowadays it is subject to many threats (poaching, by-catch). Even if there is deep growing measures for its protection, the green turtle still is an endangered species and it is listed in Appendix I of Washington Convention (CITES). In order to elaborate efficient conservation and management plans, perfect knowledge of green turtle biology, but also of its population structure and their characteristics, are needed.  In this thesis, we have assessed genetic structure of green turtle populations in the South-Western Indian Ocean by using genetic tools. In all, 1551 tissue samples have been collected from our study zone and from our control site French Polynesia (37 samples)....
Tipo: Text
Palavras-chave: Management units; Microsatellites; Mitochondrial DNA; Foraging sites; Nesting sites; South Western Indian Ocean; Population genetics; Chelonia mydas; Green turtle; Unités de gestion (MUs); Microsatellites; ADN mitochondrial; Sites d'alimentation; Sites de ponte; Génétique des populations; Chelonia mydas; Tortue verte.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/these-3532.pdf

Population Genetics of the Eastern Oyster Crassostrea virginica (Gmelin, 1791) in the Gulf of Mexico
Provedor de dados: 5
Autores: Varney, Robin L.; Galindo, Clara; Cruz, Pedro; Gaffney, Patrick M.
Genetic variation in eastern oysters (Crassostrea virginica) collected from 13 sites in the Gulf of Mexico was examined using a combination of mitochondrial DNA (mtDNA) sequencing, mtDNA restriction fragment length polymorphism analysis, and nuclear single nucleotide polymorphism analysis. Both mitochondrial and nuclear markers showed significant differentiation among samples. Combined with previous allozyme and microsatellite data, these results indicate considerable population subdivision throughout the Gulf of Mexico, despite the potentially homogenizing effect of larval dispersal.
Tipo: Text
Palavras-chave: Gulf of Mexico; Mitochondrial DNA; Single nucleotide polymorphism; Genetics; Oyster; Crassostrea virginica.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7397.pdf

Comparative phylogeography of the western Indian Ocean reef fauna
Provedor de dados: 5
Autores: Borsa, Philippe; Durand, Jean-dominique; Chen, Wei-jen; Hubert, Nicolas; Muths, Delphine; Mou-tham, Gerard; Kulbicki, Michel.
Assessing patterns of connectivity at the community and population levels is relevant to marine resource management and conservation. The present study reviews this issue with a focus on the western Indian Ocean (WIO) biogeographic province. This part of the Indian Ocean holds more species than expected from current models of global reef fish species richness. In this study, checklists of reef fish species were examined to determine levels of endemism in each of 10 biogeographic provinces of the Indian Ocean. Results showed that the number of endemic species was higher in the WIO than in any other region of the Indian Ocean. Endemic species from the WIO on the average had a larger body size than elsewhere in the tropical Indian Ocean. This suggests an...
Tipo: Text
Palavras-chave: Indo-West Pacific; Biogeographic province; Endemism; Reef fish; Mitochondrial DNA; Parsimony network.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00288/39919/44195.pdf

Phylogeography of the green turtle, Chelonia mydas, in the Southwest Indian Ocean
Provedor de dados: 5
Autores: Bourjea, Jerome; Lapegue, Sylvie; Gagnevin, L; Broderick, D; Mortimer, J.a.; Ciccione, S; Roos, David; Taquet, Coralie; Grizel, Henri.
Patterns of mitochondrial DNA (mtDNA) variation were used to analyse the population genetic structure of southwestern Indian Ocean green turtle (Chelonia mydas) populations. Analysis of sequence variation over 396 bp of the mtDNA control region revealed seven haplotypes among 288 individuals from 10 nesting sites in the Southwest Indian Ocean. This is the first time that Atlantic Ocean haplotypes have been recorded among any Indo-Pacific nesting populations. Previous studies indicated that the Cape of Good Hope was a major biogeographical barrier between the Atlantic and Indian Oceans because evidence for gene flow in the last 1.5 million years has yet to emerge. This study, by sampling localities adjacent to this barrier, demonstrates that recent gene...
Tipo: Text
Palavras-chave: Phylogeography; Mozambique Channel; Mitochondrial DNA; Indian Ocean; Control region; Chelonia mydas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2185.pdf

Mitochondrial and nuclear DNA phylogeography of  two cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud.
The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data...
Tipo: Text
Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf

Genealogy of the nuclear β-fibrinogen intron 7 in Lissotriton boscai (Caudata, Salamandridae): concordance with mtDNA and implications for phylogeography and speciation
Provedor de dados: 16
Autores: Teixeira, J.; Martínez-Solano, I.; Buckley, D.; Tarroso, P.; García-París, M.; Ferrand, N.
The power of phylogeographic inference resides in its ability to integrate information from multiple sources in an iterative hypothesis-testing framework. In this paper, we build upon previous mtDNA-based hypotheses about the evolutionary history of the Iberian newt Lissotriton boscai using sequences of the highly variable nuclear β-fibrinogen intron 7. In addition to the nuclear sequences, we produced new mtDNA data across the species range to delineate contact zones and test the congruence between nuclear and mitochondrial datasets at the same level of spatial organization. Through a combination of phylogenetic, phylogeographic continuous diffusion, and genetic landscape modelling analyses, we infer the evolutionary history of the species. We found...
Tipo: Article / Letter to the editor
Palavras-chave: Β-fibint7; Genetic landscape shape; Iberian Peninsula; Mitochondrial DNA; Nuclear DNA; 42.82; 42.64.
Ano: 2015
URL: http://www.repository.naturalis.nl/record/578951

Using ancient DNA techniques to identify the origin of unprovenanced museum specimens, as illustrated by the identification of a 19th century lion from Amsterdam
Provedor de dados: 16
Autores: Barnett, R.; Yamaguchi, N.; Shapiro, B.; Nijman, V.
In natural history collections throughout Europe, there are many old lion specimens of unknown origin. If these specimens can be shown to have originated from now-extinct populations their value would significantly increase, as would the value of the collections. Recently, a 200-year old mounted skeleton in the Zoological Museum Amsterdam has been identified as the extinct Cape lion Panthera leo melanochaita (Smith, 1842), based primarily on morphological information inferred from a painting of this specimen while it was still alive. To test this hypothesis, we used ancient DNA (aDNA) techniques to extract and sequence mitochondrial DNA (mtDNA) from this specimen, and compared the genetic results with previously published lion mtDNA sequences. Our results...
Tipo: Article / Letter to the editor
Palavras-chave: India; Mitochondrial DNA; Panthera leo; Skull morphology; South Africa; 42.84.
Ano: 2007
URL: http://www.repository.naturalis.nl/record/226454

Morphological and genetic differentiation of Bufo toads: two cryptic species in Western Europe (Anura, Bufonidae)
Provedor de dados: 16
Autores: Arntzen, J.W.; McAtear, J.; Recuero, E.; Ziermann, J.M.; Ohler, A.; Alphen, J. van; Martínez-Solano, I.
The Common toad Bufo bufo sensu lato is a widespread, morphologically conserved taxon. Recent studies have uncovered deep genetic differentiation between population groups, highlighting the need to revise the current taxonomy of the group and recognize additional species. Here we investigate patterns of variation in molecular (a mitochondrial DNA restriction enzyme assay and sequence data for two nuclear DNA fragments totalling 979 bp) and 17 morphological variables in Northern France where two of these groups meet (B. bufo sensu stricto and B. spinosus), in order to delineate their contact zone and uncover characters that would allow discrimination of the two taxa. Mitochondrial DNA data show an abrupt transition from areas where B. bufo is present to...
Tipo: Article / Letter to the editor
Palavras-chave: Bufo bufo; Bufo spinosus; Common toad; Contact zone; France; Mitochondrial DNA; Morphometrics; Nuclear DNA; 42.82.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/475530

Molecular phylogenetics and comparative anatomy of Kimberleytrachia Köhler, 2011 - a genus of land snail endemic to the coastal Kimberley, Western Australia with description of new taxa (Gastropoda, Camaenidae)
Provedor de dados: 16
Autores: Criscione, F.; Köhler, F.
Kimberleytrachia Köhler, 2011 is a genus of camaenid land snail endemic to the Western Australian Kimberley region. It comprises twelve previously recognised species, all of which occur within the high precipitation zone along the north-western coast between the Admiralty Gulf and King Sound and within less than about 50 km distance from the coast. By evaluating the variation in shell and genital anatomy as well as the differentiation in the mitochondrial markers 16S and COI, we assess the monophyly of Kimberleytrachia with respect to other camaenid genera from north-western Australia. In addition, we newly describe six species (K. jacksonensis n. sp., K. leopardus n. sp., K. nelsonensis n. sp., K. serrata n. sp., K. setosa n. sp. and K. silvaepluvialis n....
Tipo: Article / Letter to the editor
Palavras-chave: Genetic distances; Helicoidea; Mitochondrial DNA; New species; Stylommatophora; 42.73; 42.64.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/506810

Phylogenetic relationships of the European newts (genus Triturus) tested with mitochondrial DNA sequence data
Provedor de dados: 16
Autores: Zajc, I.; Arntzen, J.W.
European newts (genus Triturus) are widely studied, but their phylogeny is not yet unambiguously resolved. Fragments of mitochondrial DNA experiencing different rates of evolution (the ATPase and 12S rRNA genes) were sequenced in order to test a phylogenetic hypothesis derived from biochemical and behavioral data. Well-supported branches of the existing phylogeny gained support in our study. The monophyletic origin of the hypothesized T. boscai — T. italicus clade remained ambiguous, whereas strong support was gained for the sister-taxon relationship of T. vulgaris and T. montandoni. The position of T. vittatus as a sister taxon to the T. marmoratus species group was also supported. The phylogenetic position of T. alpestris could not be clarified. With an...
Tipo: Article / Letter to the editor
Palavras-chave: 12S rRNA; ATPase; Mitochondrial DNA; Newt; Phylogeny; Triturus.
Ano: 1999
URL: http://www.repository.naturalis.nl/record/534335

Genetic variability of the Amazon River prawn Macrobrachium amazonicum (Decapoda, Caridea, Palaemonidae)
Provedor de dados: 16
Autores: Vergamini, F.G.; Pileggi, L.G.; Mantelatto, F.L.
The freshwater prawn Macrobrachium amazonicum is widely distributed in South America, and occupies habitats with a wide range of salinities. Several investigations have revealed the existence of wide intraspecific variability among different populations, although the understanding of this variability is still fragmentary and incomplete. We compared and characterized inland and coastal populations of M. amazonicum from Brazil, using molecular data (16S and COI mtDNA) to describe the degree of variability, structure, and relationships among them. Genetic divergence rates among populations showed variability at the intraspecific level. All the analyses evidenced significant genetic divergence among populations, structuring them in three groups: I- inland...
Tipo: Article / Letter to the editor
Palavras-chave: Brazil; Genetic distance; Inland versus coastal populations; Phylogeny; Mitochondrial DNA; Population; 42.74.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/369711

Hierarchical analysis of mtDNA variation and the use of mtDNA for isopod (Crustacea: Peracarida: Isopoda) systematics
Provedor de dados: 16
Autores: Wetzer, R.
Carefully collected molecular data and rigorous analyses are revolutionizing today’s phylogenetic studies. Although molecular data have been used to estimate various invertebrate phylogenies lor more than a decade, this study is the first survey of different regions of mitochondrial DNA in isopod crustaceans assessing sequence divergence and hence the usefulness of these regions to infer phylogeny at different hierarchical levels. 1 evaluate three loci from the mitochondrial genome (two ribosomal RNAs (12S, 16S) and one protein-coding (COI)) for their appropriateness in inferring isopod phylogeny at the suborder level and below. The patterns are similar for all three loci with the most speciose suborders of isopods also having the most divergent...
Tipo: Article / Letter to the editor
Palavras-chave: Mitochondrial DNA; Isopod; Crustacea; Molecular; 12S rRNA; 16S rRNA; COI.
Ano: 2001
URL: http://www.repository.naturalis.nl/record/534397

Phylogeography, genetic diversity and structure of the poecilosclerid sponge Phorbas fictitius at oceanic islands
Provedor de dados: 16
Autores: Xavier, J.R.; Soest, R.W.M. van; Breeuwer, J.A.J.; Martins, A.M.F.; Menken, S.B.J.
In this study we assessed the sequence variation in the I3-M11 partition of the mtDNA cytochrome c oxidase subunit I gene (COI) in ten populations of the Atlanto-Mediterranean demosponge Phorbas fictitius (Porifera: Poecilosclerida) at two spatial scales: a regional scale comparing mainland (Iberian) and insular (Macaronesian) populations, and a local (Archipelagic) scale focusing on different island populations of the Azores archipelago. A multiple approach combining diversity measures, FST estimates, phylogenetic inference and nested clade phylogeographic analysis was used to assess the genetic structure and elucidate the evolutionary history of this species. Genetic differentiation, based of FST estimates, was found among most populations at both scales...
Tipo: Article / Letter to the editor
Palavras-chave: Azores; Genetic diversity; Iberia; Macaronesia; Mitochondrial DNA; Porifera; 42.72.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/355610

The value of PCR-RFLP molecular markers for the differentiation of immature stages of two necrophagous flies (Diptera: Calliphoridae) of potential forensic importance
Provedor de dados: 82
Autores: Thyssen,Patricia J.; Lessinger,Ana C.; Azeredo-Espin,Ana M.L.; Linhares,Arício X.
The identification of insect species involved in corpses decomposition is of particular importance in estimating the post-mortem interval (PMI) in forensic science, since the PMI is based on information about the life cycle of necrophagous insects. However, the identification of some insect species, especially in their immature stages, may be complicated by many factors, even for experienced taxonomists. Species of the same genus such as Hemilucilia segmentaria (Fabricius) and H. semidiaphana (Rondani) (Diptera: Calliphoridae) are morphologically and behaviorally very similar, but differ in their growth and maturation rates. These species are abundant in forests, exclusively necrophagous and, therefore, are of potential medicolegal importance for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forensic entomology; Mitochondrial DNA; Hemilucilia segmentaria; Hemilucilia semidiaphana.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000500009

A new species of Tetragonopterus Cuvier, 1816 (Characiformes: Characidae: Tetragonopterinae) from the rio Jari, Amapá, northern Brazil
Provedor de dados: 83
Autores: Melo,Bruno F; Benine,Ricardo C; Mariguela,Tatiane C; Oliveira,Claudio.
A new species of Tetragonopterus is described from the rio Jari, a tributary to the left margin of rio Amazonas, at the border between Amapá and Pará States, northern Brazil. It is morphologically diagnosed from the other species of the genus (T. argenteus, T. chalceus, and T. rarus new combination) by the lozenge-shaped spot on the caudal peduncle vs. rounded to square spot on the other species. Partial sequences of the mitochondrial gene Cytochrome Oxidase C subunit I, from representatives of all valid species of Tetragonopterus, including this new species, were analyzed. The obtained results revealed a significant genetic distance between the new species and its congeners. A discussion on the new combination, Tetragonopterus rarus, is also provided.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COI gene; Genetic distance; Mitochondrial DNA; Neotropical fish; Taxonomy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100002

A Century after! Rediscovery of the ancient catfish Diplomystes Bleeker 1858 (Siluriformes: Diplomystidae) in coastal river basins of Chile and its implications for conservation
Provedor de dados: 83
Autores: Muñoz-Ramírez,Carlos P.; Briones,Raul; Colin,Nicole; Fierro,Pablo; Górski,Konrad; Jara,Alfonso; Manosalva,Aliro.
ABSTRACT The ancient catfish family Diplomystidae, with seven species endemic to rivers of southern South America, represents one of the oldest branches of the diverse order Siluriformes. With most species endangered, new reports of these species become extremely valuable for conservation. Currently, it is assumed that Diplomystes species inhabit only Andean (large) basins, and that they are extinct from coastal (small) basins from which their presence have not been recorded since 1919. Here, we document new records of the family Diplomystidae in the Laraquete and Carampangue basins, two coastal basins from the Nahuelbuta Coast Range, Chile, with no previous reports. This finding represents the rediscovery of the genus in coastal basins in more than a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Management Unit; Mitochondrial DNA; Rare Species; Streams; Threatened Species.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000100208

Color pattern variation in Cichla temensis (Perciformes: Cichlidae): resolution based on morphological, molecular, and reproductive data
Provedor de dados: 83
Autores: Reiss,Paul; Able,Kenneth W.; Nunes,Mario S.; Hrbek,Tomas.
Morphological variants of Cichla temensis, readily differentiated by their striking color pattern differences, are found in several Amazon basin flood pulse river systems. The adult variants have at times been thought to represent different species or sexual dimorphism. A three part study was performed in two regions in Brazil (rio Igapó Açú and rio Caures) to elucidate the nature of the variants. In part one; selected diagnostic morphometric characters were compared intraspecifically among the variants and interspecifically with C. monoculus and C. orinocensis. All of the C. temensis variants were found to differ significantly from their sympatric congeners while not differing among each other. In part two, mitochondrial DNA samples were compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gonadosomatic index; Mitochondrial DNA; Neotropical fish; Secondary sexual characteristics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000100006

Population genetic structure and body shape assessment of Pagrus pagrus (Linnaeus, 1758) (Perciformes: Sparidae) from the Buenos Aires coast of the Argentine Sea
Provedor de dados: 83
Autores: Porrini,Leonardo P.; Iriarte,Pedro J. Fernández; Iudica,Celia M.; Abud,Eddie Aristizabal.
This study highlights the analysis of the morphological and genetic variation of the common sea bream Pagrus pagrus, and compares its two main areas of concentration: the northern (35ºS - 38ºS) and the southern areas (39ºS - 41ºS) of the Buenos Aires coast of the Argentine Sea. Body shape characterization presented two significantly different morphotypes (Wilks' Lambda=0.224, P&lt;0.001). Northern individuals displayed a higher middle area while southern ones were smaller and their caudal peduncle was shorter. The northern and southern areas did not yield significant genetic differences either with the control region or the microsatellite loci, revealing that P. pagrus is not genetically structured. However, individuals from these areas should not be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geometric morphometrics; Management unit; Mitochondrial DNA; Stocks.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000200431

A new glanapterygine catfish of the genus Listrura (Siluriformes: Trichomycteridae) from southeastern Brazil, corroborated by morphological and molecular data
Provedor de dados: 83
Autores: Villa-Verde,Leandro; Lazzarotto,Henrique; Lima,Sergio M. Q.
Listrura costai, new species, is described from small streams in a swampy coastal plain in the rio Jurumirim basin, Angra dos Reis Municipality, Rio de Janeiro State, southeastern Brazil. The new species is morphologically very similar to L. nematopteryx and L. picinguabae, all possessing only one long pectoral-fin ray. It differs from its congeners by possessing an autapomorphic character: first hypobranchial with an anterior process (vs. process absent). Other features such as coloration, numbers of opercular and interopercular odontodes, number of anal-fin rays, head length, and shape of some bone structures help to distinguish the new species from L. nematopteryx and L. picinguabae. Molecular analyses using partial sequences of the mitochondrial DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic rain forest; Genetic distance; Mitochondrial DNA; Taxonomy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300005

Lineage Classification of Canine Inheritable Disorders Using Mitochondrial DNA Haplotypes
Provedor de dados: 19
Autores: TAKAHASI, Shoko; MIYAHARA, Kazuro; ISHIKAWA, Hirosi; ISHIGURO, Naotaka; SUZUKI, Mitsuyoshi; 宮原, 和郎; 鈴木, 三義.
To estimate the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders, 600 base pairs of mtDNA D-loop region were amplified from 365 dogs and were determined for mtDNA sequences. The diversity of the 600-bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV. Lineage analysis using the mtDNA haplotype indicated that each dog breed genetically comprises one or a few mtDNA haplotypes. When the relationship between genetic background and occurrences of clinical diseases was estimated, canine lineage analysis using mtDNA haplotype revealed that the disorders distributed in the dominant mtDNA haplotypes of...
Palavras-chave: D-loop; Haplotype lineage analysis; Mitochondrial DNA.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4066

Mitochondrial Genetic Characterization Of Bluefin Tuna (Thunnus Thynnus) From Three Mediterranean (Libya, Malta, Tunisia); And One Atlantic Locations (Gulf Of Cadiz).
Provedor de dados: 20
Autores: Farrugia, A.F.; Pla, C.; Viñas, J.; Tawil, M.Y.; Hattour, A.; de la Serna, J.M.
None of the previous genetic analysis found significant differences within the Mediterranean. However, a more exhaustive analysis is needed inside this sea. In this sense, we have analyzed the mitochondrial control region sequence variability of three Mediterranean locations: Libya (n = 22), Tunisia (n = 23) and Malta (n = 12). Additionally a sample from the Gulf of Cadiz (n = 24) in the East Atlantic Ocean was also included in the study. The AMOVA analysis revealed slightly (Fst = 0.013) but significative genetic differences (P &lt; 0.000) for the Malta location, probably due to its small sample size (n = 12). In the other hand, when this location is removed from the analysis, no differences where found among the three remaining location (Fst = 0,005; P =...
Tipo: Journal Contribution
Palavras-chave: Atlantic ocean; Mitochondrial DNA; Bluefin tuna; Sequencing; Genetic characterization; Mediterranean sea; Tuna fisheries.
Ano: 2003
URL: http://hdl.handle.net/1834/792

Cytogenetic and molecular analysis of the Pantaneiro cattle breed
Provedor de dados: 86
Autores: Issa,Érica Cunha; Jorge,Wilham; Sereno,José Robson Bezerra.
The objective of this work was to characterize Pantaneiro cattle genetically through its paternal ancestry by the morphology of the Y chromosome, whether submetacentric or acrocentric, as well as to identify the maternal ancestry through mitochondrial DNA. The karyotype and mitochondrial DNA of 12 bulls of Pantaneiro breed were analyzed. The Y chromosome was analyzed in lymphocyte metaphases and the mitochondrial DNA by diagnosing its haplotype (Bos taurus and Bos indicus). Among Pantaneiro animals analyzed three had a taurine (submetacentric) Y and nine had a zebuine (acrocentric) Y chromosome, suggesting breed contamination by Zebu cattle, once Pantaneiro is considered to be of European origin. The mitochondrial DNA was exclusively of taurine origin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos taurus taurus; Bos taurus indicus; Y chromosome; Mitochondrial DNA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001100006

Genetic Diversity and Phyletic Evolution of Eleven Chinese Indigenous and Three Commercial Chicken Breeds by mtDNA Sequences
Provedor de dados: 89
Autores: Yu,C; Qiu,M; Jiang,X; Zhang,Z; Du,H; Li,Q; Xia,B; Song,X; Hu,C; Xiong,X; Yang,L; Peng,H; Chen,J; Wang,Y; Yang,C.
ABSTRACT Chinese indigenous chicken breeds are geographically widespread, and a total of 116 indigenous chicken breeds are listed as Chinese national genetic resources. However, these indigenous chicken breeds are facing serious challenges as declining population and germplasm degeneration because lots of commercial chicken breeds had been introduced. In this study, the genetic variations of eleven Chinese indigenous chicken breeds of Sichuan province and three commercial chicken breeds were investigated based on the partial mitochondrial DNA D-loop of 487bp in length. 147 individuals from 14 breeds were examined and 34 haplotypes were observed. Genetic diversity analysis showed that the highest haplotype diversity level was found in Dahen Chicken (DH)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chinese chicken; Mitochondrial DNA; Cluster analysis; Gene flow; Phylogeny.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400308

Genetic differentiation in populations of Aedes aegypti (Diptera, Culicidae) dengue vector from the Brazilian state of Maranhão
Provedor de dados: 92
Autores: Sousa,Andrelina Alves de; Fraga,Elmary; Sampaio,Iracilda; Schneider,Horacio; Barros,Maria Claudene.
ABSTRACT Aedes (Stegomyia) aegypti is the vector responsible for the transmission of the viruses that cause zika, yellow and chikungunya fevers, the four dengue fever serotypes (DENV - 1, 2, 3, 4), and hemorrhagic dengue fever in tropical and subtropical regions around the world. The present study investigated the genetic differentiation of the 15 populations of this vector in the Brazilian state of Maranhão, based on the mitochondrial ND4 marker. A total of 177 sequences were obtained for Aedes aegypti, with a fragment of 337 bps, 15 haplotypes, 15 polymorphics sites, haplotype diversity of h = 0.6938, and nucleotide diversity of π = 0.01486. The neutrality tests (D and Fs) were not significant. The AMOVA revealed that most of the variation (58.47%) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene flow; Mitochondrial DNA; ND4.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262017000100051

Genetic variability of a population of Aedes aegypti from Paraná, Brazil, using the mitochondrial ND4 gene
Provedor de dados: 92
Autores: Twerdochlib,Adriana L.; Bonna,Ana C. Dalla; Leite,Selene S.; Chitolina,Rodrigo F.; Westphal,Betina; Navarro-Silva,Mario A.
Genetic variability of a population of Aedes aegypti from Paraná, Brazil, using the mitochondrial ND4 gene. To analyze the genetic variability of populations of Aedes aegypti, 156 samples were collected from 10 municipalities in the state of Paraná, Brazil. A 311 base pairs (bp) region of the NADH dehydrogenase subunit 4 (ND4) mitochondrial gene was examined. An analysis of this fragment identified eight distinct haplotypes. The mean genetic diversity was high (h = 0.702; p = 0.01556). AMOVA analysis indicated that most of the variation (67%) occurred within populations and the F ST value (0.32996) was highly significant. F ST values were significant in most comparisons among cities. The isolation by distance was not significant (r = -0.1216 and p = 0,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes aegypti; Genetic variability; Mitochondrial DNA; Genetic population; State of Paraná.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262012000200013

Organization and variation of mitochondrial DNA control region in pleurodiran turtles
Provedor de dados: 103
Autores: Wang,Ling; Zhou,Xuming; Nie,Liuwang.
Three complete mitochondrial DNA (mtDNA) control regions (CRs) of Chelodina rugosa (Ogilby, 1890), Chelus fimbriata (Schneider, 1783), and Podocnemis unifilis (Troschel, 1848) were firstly determined using Long-PCR method and the length were 1,016 bp, 1,149 bp, and 985bp, respectively. Together with CRs of Pelomedusa subrufa (Bonnaterre, 1789) and nearly complete CR of Podocnemis expansa (Schweigger, 1812) obtained from GenBank, the structural and evolutionary characteristics of mtDNA CRs in pleurodiran turtle were analyzed in this study. We identified three functional domains (TAS, CD, and CSB domains) as well as their conservation sequences (TAS, CSB-F, and CSB-1) according to their homology to those of other turtles. Within the TAS domain, an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Control region; CSB; Mitochondrial DNA; Molecular evolution; Pleurodira.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000400011

Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia
Provedor de dados: 103
Autores: Aguirre-Obando,Oscar A.; Bona,Ana C. Dalla; Duque L.,Jonny E.; Navarro-Silva,Mário A.
Mosquito control prevails as the most efficient method to protect humans from the dengue virus, despite recent efforts to find a vaccine for this disease. We evaluated insecticide resistance and genetic variability in natural populations of Aedes aegypti (Linnaeus, 1762) from Colombia. This is the first Colombian study examining kdr mutations and population structure. Bioassays with larvae of three mosquito populations (Armenia, Calarcá and Montenegro) were performed according to the World Health Organization (WHO) guidelines, using Temephos. For the analysis of the Val1016Ile mutation and genetic diversity, we sampled recently-emerged adults from four mosquito populations (Armenia, Calarcá, Montenegro and Barcelona). Following the WHO protocol, bioassays...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioassays; Gene flow; Knockdown resistance; Mitochondrial DNA; ND4 gene.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000100014

Genetic profile and molecular resistance of Aedes (Stegomyia) aegypti (Diptera: Culicidae) in Foz do Iguaçu (Brazil), at the border with Argentina and Paraguay
Provedor de dados: 103
Autores: Bona,Ana C. Dalla; Piccoli,Carla F.; Leandro,André de S.; Kafka,Rosinei; Twerdochilib,Adriana L.; Navarro-Silva,Mário A.
The genetic variability of populations of Aedes (Stegomyia) aegypti (Linnaeus, 1762) is often low due to the intense selection pressure caused by chemical control measures. In this study, we evaluated the susceptibility of larvae and adults of this mosquito to chemical insecticides, the frequency of the Val1016IIe mutation, and the genetic variability of the mitochondrial ND4 gene fragment in the urban area of Foz do Iguaçu, Paraná. The populations of A. aegypti in the southern and central regions of the city were resistant to the diagnostic dose of temephos 0.0162 ppm. Additionally, we detected difference in susceptibility in the northern population. The resistance ratios (RR95) were 3.8, 4.1, and 4.6 for the populations in the north, central, and south...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Knockdown resistance; Mitochondrial DNA; Pyrethroids; Temephos.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702012000600005

Mitochondrial and Y chromosome genetic diversity in the Portuguese Lidia bovine breed
Provedor de dados: 96
Autores: Correia,Pedro Bettencourt Cardoso; Baron,Erica Elias; Silva,Joaquim Fernando Moreira da; Gardyn,Óscar Cortés.
ABSTRACT A total of 80 samples were collected to analyze the mitochondrial DNA (mtDNA) and Y chromosome genetic diversity of two main Portuguese Lidia bovine populations and clarify their genetic relationships with Spanish Lidia lineages. A 521-bp D-loop fragment was sequenced in 40 animals belonging to four herds (three Brava dos Açores and one Casta Portuguesa) and the same number of samples were collected with equal distribution from males in order to genotype six microsatellites, one single nucleotide polymorphism, and one indel. The mtDNA diversity recorded was similar to that observed in Lidia cattle. Haplotype T3 was the most common (62.5%), followed by the African T1 haplotype (25%); very low frequencies were recorded for haplotypes T2 (2.5%) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lidia cattle; Mitochondrial DNA; Portuguese lineages; Y chromosome.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000200099

Genetic diversity of the Brazilian Creole cattle Pé-duro assessed by microsatellites and mitochondrial DNA
Provedor de dados: 96
Autores: Oliveira,Ana Paula Ferreira de; Carvalho,José Herculano de; Miretti,Marcos Mateo; Lara,Maria Aparecida Cassiano; Contel,Eucleia Primo Betioli.
The objective of this study was to describe the genetic diversity and structure of the largest Pé-duro population by assessing variation at ten autosomal microsatellite (STR) loci and mitochondrial DNA (mtDNA) sequences. The mean expected heterozygosity was 0.755, the mean observed heterozygosity was 0.600 and significant inbreeding coefficient (Fis) and deviations from the Hardy-Weinberg equilibrium in most of analyzed loci demonstrate the impact of inbreeding and homozygosis on this population. A more in-depth genetic analysis could be achieved by expanding the STR list. The analysis of mtDNA provided evidence of ancestral African taurine haplotypes in Pé-duro and excluded maternal Zebuine introgression. In this report, the main Pé-duro population is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos taurus; Cattle; Conservation; Genetic diversity; Microsatellites; Mitochondrial DNA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001100003

GENETIC STATUS, SOURCE AND ESTABLISHMENT RISK OF THE GIANT TIGER SHRIMP (PENAEIDAE: Penaeus monodon), AN INVASIVE SPECIES IN COLOMBIAN CARIBBEAN WATERS
Provedor de dados: 37
Autores: AGUIRRE-PABÓN,J.C; OROZCO BERDUGO,G. Jr; NARVÁEZ BARANDICA,J.C.
The tiger shrimp (Penaeus monodon) is an Indo-Pacific species. Its global production between 1970 and 1980 exceeded all other shrimp species, which favored its introduction and cultivation outside its natural range in several countries of Africa, Europe, USA and South America. It is currently found in the coast of the Atlantic Ocean (Mexico, United States, Puerto Rico, Brazil, Guyana, Venezuela and Colombia). Despite the risk involved, no studies have been conducted to evaluate their impact as a possible invasive species and their genetic condition. This study evaluated the genetic status and population origin of P. monodon in the northernmost Colombian Caribbean, analyzing the mitochondrial DNA control region (mtDNA-CR). 16 individuals were randomly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological invasion; Caribbean Sea; Founder effect; Genetic relationships; Mitochondrial DNA.
Ano: 2015
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100013

Identificação molecular de Cichla (Perciformes: Cichlidae) introduzidas nos reservatórios do Sul do Brasil. Molecular identification of Cichla (Perciformes: Cichlidae) introduced in the reservoirs of the South Region in Brazil
Provedor de dados: 3
Autores: Briñez, Boris; Universidade Estadual de Maringá; Júlio Júnior, Horacio Ferreira; Universidade Estadual de Maringá; Prioli, Sonia Maria Alves Pinto; Universidade Estadual de Maringá; Maniglia, Thiago Cintra; Núcleo de Pesquisas em Limnologia, Ictiologia e Aqüicultura (Nupelia), Universidade Estadual de Maringá; Prioli, Alberto José; Universidade Estadual de Maringá.
Espécies de tucunaré foramn introduzidas em inúmeras bacias hidrográficas da América do Sul e em outras regiões do planeta, principalmente devido as suas características esportivas, de peixe lutador. Revisão recente das espécies do gênero Cichla mostraram que as espécies que foram introduzidas nos reservatórios das regiões Sul, Sudeste e Nordeste, são duas espécies novas, descritas como Cichla kelberi (tucunaré amarelo) e Cichla piquiti (tucunaré azul) identificadas erroneamente como C. monoculus e C. ocellaris. Com o objetivo de identificar as populações de Cichla presentes no Rio Paranapanema e Paraná, foram sequenciadas um total de 323 pares de bases (pb) da região controle (MtDNA) obtidas de oitenta e quatro indivíduos de Cichla em cinco localidades...
Palavras-chave: 2.02.02.00-8 species introduction; C. monoculus; Mitochondrial DNA; Peacock bass; Paraná river Genética Species introduction  C.monoculus  MtDNA control region  Peacock bass  Upper Paraná River  Tucunaré. Introdução de espécies  C.monoculus  região controle (MtDNA)  Tucunaré Genética.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/10684

Innovative molecular approach to the identification of Colossoma macropomum and its hybrids
Provedor de dados: 42
Autores: Gomes,Fátima; Schneider,Horacio; Barros,Claudene; Sampaio,Dioniso; Hashimoto,Diogo; Porto-Foresti,Fábio; Sampaio,Iracilda.
Tambaqui (Colossoma macropomum) is the fish species most commonly raised in the Brazilian fish farms. The species is highly adaptable to captive conditions, and is both fast-growing and relatively fecund. In recent years, artificial breeding has produced hybrids with Characiform species, known as "Tambacu" and "Tambatinga". Identifying hybrids is a difficult process, given their morphological similarities with the parent species. This study presents an innovative molecular approach to the identification of hybrids based primarily on Multiplex PCR of a nuclear gene (α-Tropomyosin), which was tested on 93 specimens obtained from fish farms in northern Brazil. The sequencing of a 505-bp fragment of the Control Region (CR) permitted the identification of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pisciculture; Multiplex PCR; Mitochondrial DNA; Genetic bottleneck.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200020

Genetic variation in native and farmed populations of Tambaqui (Colossoma macropomum) in the Brazilian Amazon: regional discrepancies in farming systems
Provedor de dados: 42
Autores: Aguiar,Jonas; Schneider,Horacio; Gomes,Fátima; Carneiro,Jeferson; Santos,Simôni; Rodrigues,Luis R.; Sampaio,Iracilda.
The tambaqui, Colossoma macropomum, is the most popular fish species used for aquaculture in Brazil but there is no study comparing genetic variation among native and farmed populations of this species. In the present study, we analyzed DNA sequences of the mitochondrial DNA to evaluate the genetic diversity among two wild populations, a fry-producing breeding stock, and a sample of fish farm stocks, all from the region of Santarém, in the west of the Brazilian state of Pará. Similar levels of genetic diversity were found in all the samples and surprisingly the breeding stock showed expressive representation of the genetic diversity registered on wild populations. These results contrast considerably with those of the previous study of farmed stocks in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tambaqui; Mitochondrial DNA; Genetic variability; Aquaculture.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401439

Range distribution and contributions to taxonomy of Elops smithi (ELOPIFORMES: ELOPIDAE)
Provedor de dados: 42
Autores: SOUSA,RODRIGO P.C. DE; SODRÉ,DENISE; COSTA,RAUQUÍRIO M. DA; VALLINOTO,MARCELO; OLIVEIRA,EDIVALDO H.C.; SILVA-OLIVEIRA,GLÁUCIA C.; SAMPAIO,IRACILDA; GUIMARÃES-COSTA,AURYCÉIA.
Abstract: This study presents the first record of Elops smithi for northern Brazil. The evidence suggests this species is being misidentified incorrectly as Elops saurus in estuaries of the Western Atlantic Ocean. Here, morphological, molecular, and cytogenetic ﻿evidence identified all ladyfish specimens from one estuary in the region as E. smithi. ﻿Thus, at least Elops smithi occurs in the northern coast of Brazil and it is recommended that specimens from this region identified as E. saurus be further investigated with genetic and cytogenetic tools in order to assure a correct species identification.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elopidae; Karyotype; Ladyfish; Malacho; Mitochondrial DNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700622

High passage numbers induce resistance to apoptosis in C2C12 muscle cells
Provedor de dados: 47
Autores: Pronsato,Lucia; La Colla,Anabela; Ronda,Ana Carolina; Milanesi,Lorena; Boland,Ricardo; Vasconsuelo,Andrea.
Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (&lt;20) or high (&amp;gt;60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Over-subculturing changes; Aged muscle cultures; Mitochondrial DNA.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100001

Muscle cell outside and inside: the nascent approach of a clinician
Provedor de dados: 48
Autores: FADIC,RICARDO.
Understanding muscle cell in disease and health is an unfinished process. Following the lead of Jaime Alvarez, I have had the opportunity of working on two complementary approaches to this field. One is the study of muscle cell surface molecules. Both synaptic muscle molecules, such as the asymmetric form of acetylcholinesterase, and extrasynaptic molecules, such as the extracellular matrix proteoglycans, are regulated by the motor nerve activity. This illustrates one of Jaime's teachings: cell phenotypes are a dynamic process that reflects the influence of other cells (&lt;A HREF="#0"&gt;Alvarez, 2001&lt;/A&gt;). Proteoglycans have many functions, including growth factor receptors. Studying them in muscular dystrophy will contribute to the comprehension...
Tipo: Journal article
Palavras-chave: Mitochondrial DNA; Muscle disease; Muscular dystrophy; Proteoglycans.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200018

Successful amplification of mitochondrial DNA from dentin of the bottlenose dolphin Tursiops truncatus
Provedor de dados: 52
Autores: Weber,Laura Isabel; Luca,Michelle Jaccottet de; Barreto,André Silva; Souza,Tiago Tolentino de.
Twenty-four teeth of Tursiops truncatus from the collection of the Marine Mammals Laboratory (FURG-RS) were analysed for their potential as DNA source for population genetic and taxonomic studies. All teeth were submitted to decontamination, grinding and demineralisation processes. Dentin powder was weighed for DNA extraction, which was evaluated by the amplification of two mitochondrial genes through PCR using universal primers. Cytochrome-b gene was amplified successfully. PCR products showed concentrations of 3-12 ng/µl for dentin powder, which was positively correlated to the quantity of dentin powder used for DNA extraction. To rule out contamination a SSCP was carried out including positive controls, and samples of humans involved in the analysis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dolphins; Tursiops; Museum specimens; Dentin; Mitochondrial DNA; Cytochrome-b.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100002

Genetic structure of Brazilian populations of white mouth croaker Micropogonias furnieri (Perciformes : Sciaenidae)
Provedor de dados: 52
Autores: Puchnick-Legat,Angela; Levy,José Alberto.
Mitochondrial (mt) DNA population structure was examined by using restriction fragment length polymorphism analysis (RFLP) among 149 white mouth croakers (Micropogonias furnieri) sampled from six locations in the Brazilian coast. Heterogeneity tests revealed no differentiation in mtDNA haplotype frequencies within the region between 23°S and 34°S (P=0.263), but significant heterogeneity was detected between north and south of 23°S (P&lt;0.003). Analysis of molecular variance (AMOVA) indicated a low F ST value (0.008, P=0.180) among south-central localities (23-34°S), but a significant level of population subdivision (F ST = 0.322, P&lt; 0.003) between north and south-central localities. UPGMA analysis of mtDNA sequence divergence revealed differentiation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic population structure; Mitochondrial DNA; RFLP; Micropogonias furnieri.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400011

Species determination of Brazilian mammals implicated in the epidemiology of rabies based on the control region of mitochondrial DNA
Provedor de dados: 55
Autores: Carnieli Junior,Pedro; Fahl,Willian de Oliveira; Castilho,Juliana Galera; Brandão,Paulo Eduardo; Carrieri,Maria Luiza; Kotait,Ivanete.
Identification of animals that are decomposing or have been run over or burnt and cannot be visually identified is a problem in the surveillance and control of infectious diseases. Many of these animals are wild and represent a valuable source of information for epidemiologic research as they may be carriers of an infectious agent. This article discusses the results obtained using a method for identifying mammals genetically by sequencing their mitochondrial DNA control region. Fourteen species were analyzed and identified. These included the main reservoirs and transmitters of rabies virus, namely, canids, chiroptera and primates. The results prove that this method of genetic identification is both efficient and simple and that it can be used in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Sequencing; Identification; Mammals; Rabies.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600002

Prevalence of the A1555G (12S rRNA) and tRNA Ser(UCN) mitochondrial mutations in hearing-impaired Brazilian patients
Provedor de dados: 56
Autores: Abreu-Silva,R.S.; Lezirovitz,K.; Braga,M.C.C.; Spinelli,M.; Pirana,S.; Della-Rosa,V.A.; Otto,P.A.; Mingroni-Netto,R.C.
Mitochondrial mutations are responsible for at least 1% of the cases of hereditary deafness, but the contribution of each mutation has not yet been defined in African-derived or native American genetic backgrounds. A total of 203 unselected hearing-impaired patients were screened for the presence of the mitochondrial mutation A1555G in the 12S rRNA gene and mutations in the tRNA Ser(UCN) gene in order to assess their frequency in the ethnically admixed Brazilian population. We found four individuals with A1555G mutation (2%), which is a frequency similar to those reported for European-derived populations in unselected samples. On the other hand, complete sequencing of the tRNA Ser(UCN) did not reveal reported pathogenic substitutions, namely A7445G,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Hearing impairment; A1555G mutation; TRNA Ser(UCN) mutations; European- and African- Brazilian patients.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200008

Genetic Relationships of Common Bean (Phaseolus vulgaris L.) Race Chile with Wild Andean and Mesoamerican Germplasm
Provedor de dados: 117
Autores: Becerra V,Viviana; Paredes C,Mario; Debouck,Daniel.
The Chilean common bean (Phaseolus vulgaris L.) belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Thirty-two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (Tórtola, Coscorrón, and...
Tipo: Journal article
Palavras-chave: Chilean common bean germplasm; Chloroplast DNA; Mitochondrial DNA; PCR-RFLP.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100001