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Mycophenolate mofetil versus Rapamycin in Han: SPRD rats with Polycystic Kidney Disease
Provedor de dados: 48
Autores: ZHANG,TONG; WANG,LI; XIONG,XISHAN; MAO,ZHIGUO; WANG,LIMING; MEI,CHANGLIN.
Objective: We aimed to explore the effect of Mycophenolate mofetil (MMF) on loss of renal function and cyst progression compared to rapamycin in Han: SPRD rats. We also sought to assess whether the effect of combination therapy of MMF plus rapamycin was better than that of monotherapy. Methods: Sixty heterozygous (Cy/+) and littermate control (+/+) male Han: SPRD rats were weaned at 4 weeks of age, then divided into four groups randomly to receive different treatments by intragastric administration for 2 months: vehicle-treated group as control, MMF-treated group (20mg/kg/day), rapamycin-treated group (2mg/kg/day), and MMF+Rapa- treated group (MMF 20mg/kg/day plus Rapamycin 2mg/kg/day). Resulls: After 2 months of treatment, rapamycin caused a 22 % decrease...
Tipo: Journal article
Palavras-chave: ADPKD; Han: SPRD rat; Mycophenolate mofetil; Rapamycin.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400005

Department of International Relations of CONICYT
Provedor de dados: 48
Autores: ALTAMIRANO,XAVIER; VELIT,CECILIA.
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500001

Effects of CpG oligodeoxynucleotide 1826 on acute radiation-induced lung injury in mice
Provedor de dados: 48
Autores: Li,Xuan; Xu,Guoxiong; Qiao,Tiankui; Yuan,Sujuan; Zhuang,Xibing.
BACKGROUND: The radiation-induced lung injury is a common complication from radiotherapy in lung cancer. CpG ODN is TLR9 activator with potential immune modulatory effects and sensitization of radiotherapy in lung cancer. This study aimed to examine the effect of CpG ODN on acute radiation-induced lung injury in mice. METHODS AND RESULTS: The mouse model of radiation-induced lung injury was established by a single dose of 20 Gy X-rays exposure to the left lung. The results showed that the pneumonia score was lower in RT+CpG group than in RT group on 15th and 30th days. Compared with RT group, CpG ODN reduced the serum concentrations of MDA (P &lt; 0.05) and increased the serum concentrations of SOD, GSH (P &lt; 0.05). The serum concentration of...
Tipo: Journal article
Palavras-chave: CpG ODN; Acute radiation-induced lung injury; Reactive oxygen species.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100008

Characterization of the long-terminal repeat single-strand tail-binding site of Moloney-MuLV integrase by crosslinking
Provedor de dados: 48
Autores: VERA,JORGE; VALENZUELA,BEATRIZ; ROTH,MÓNICA J; LEÓN,ÓSCAR.
Processing of viral DNA by retroviral integrase leaves a dinucleotide single-strand overhang in the unprocessed strand. Previous studies have stressed the importance of the 5' single-stranded (ss) tail in the integration process. To characterize the ss-tail binding site on M-MuLV integrase, we carried out crosslinking studies utilizing a disintegration substrate that mimics the covalent intermediate formed during integration. This substrate carried reactive groups at the 5' ss tail. A bromoacetyl derivative with a side chain of 6 A was crosslinked to the mutant IN 106-404, which lacks the N-terminal domain, yielding a crosslinked complex of 50 kDa. Treatment of IN 106-404 with N-ethylmaleimide (NEM) prevented crosslinking, suggesting that Cys209 was...
Tipo: Journal article
Palavras-chave: Integrase; Retrovirus; Crosslinking.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100009

IX REUNION ANUAL  SOCIEDAD DE ECOLOGIA DE CHILE
Provedor de dados: 48
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100011

Contribution of dendritic cell/T cell interactions to triggering and maintaining autoimmunity
Provedor de dados: 48
Autores: Llanos,Carolina; Carreño,Leandro J; Kalergis,Alexis M.
Under healthy conditions, there is a balance between tolerance to self-tissue constituents and immunity against foreign antigens. Autoimmunity diseases (AD) take place when that equilibrium is disrupted and the immune response is directed to self-antigens, leading to injury or destruction of host tissues. The mechanisms conducing to the loss of immune tolerance remain largely unknown. The recent appearance of biological therapies has contributed to significant reduction in morbidity. However, currently available therapies are associated with important side effects and work only as palliative treatments. Dendritic cells (DCs) have emerged as key players in developing and maintaining adaptive immunity due to their capacity to prime and modulate T cell...
Tipo: Journal article
Palavras-chave: Dendritic cells; T cells; Immunological synapse; Autoimmunity; Tolerance.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100007

The effects of Tynnanthus fasciculatus (Bignoniaceae) infusion on testicular parenchyma of adult Wistar rats
Provedor de dados: 48
Autores: Melo,Fabiana C. S. A; Matta,Sérgio L. P; Paula,Tarcízio A. R; Gomes,Marcos L. M; Oliveira,Luciana C.
Traditional medicine provides strong guidance for scientific experiments involving plant products used by the Brazilian people. The species "cipó-cravo" (Tynnanthus fasciculatus) is a plant commonly used either to combat indigestion and stomachaches, or as a general stimulant and aphrodisiac. In this study, the effects of "cipó-cravo" infusion were investigated within the testicular parenchyma of adult Wistar rats. Rats were divided into 3 groups: a control (distilled water) and two treated groups, which received the plant infusion (100 and 200mg/animal/day). The 200mg dose promoted a significant increase of the testicular parenchyma weight and of the volume and total length of the seminiferous tubules, as well as in total daily sperm production and sperm...
Tipo: Journal article
Palavras-chave: Germ cell; Sertoli cell; Sperm production; Spermatogenesis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400009

Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak
Provedor de dados: 48
Autores: CÓRDOVA,JOSÉ LUIS; ASTORGA,JOSEFA; SILVA,WALLY; RIQUELME,CARLOS.
Between November 1997 and April 1998, several human gastroenteritis cases were reported in Antofagasta, a city in the north of Chile. This outbreak was associated with the consumption of shellfish, and the etiologic agent responsible was identified as Vibrio parahaemolyticus. This was the first report of this bacterium causing an epidemic in Chile. V. parahaemolyticus was the only pathogenic bacterium isolated from patient stools and from shellfish samples. These isolates were analyzed by polymerase chain reaction (PCR) amplification of the pR72H gene, a species-specific sequence. Based on the pR72H gene amplification pattern, at least three different isolates of V. parahaemolyticus were found. Two isolates (named amplicons A and C) generated PCR products...
Tipo: Journal article
Palavras-chave: Bacterial bloom; Bacterial geographical dispersion; PCR amplification; Molecular typification.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300017

Erratum
Provedor de dados: 48
Tipo: Journal article
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400015

The cytotoxic and antileishmanial activity of extracts and fractions of leaves and fruits of Azadirachta indica (A Juss.)
Provedor de dados: 48
Autores: Carneiro,Sabrina M. P; Carvalho,Fernando A. A; Santana,Lorena C. L. R; Sousa,Alessian P. L; Neto,José M. M; Chaves,Mariana H.
The leishmaniases are severe parasitic diseases that occur worldwide, caused by protozoa of the genus Leishmania. Studies with medicinal plants can lead to a range of possibilities for treating and improving the patients' quality of life. Research on Azadirachta indica fractions and extracts has shown that they have excellent anti-leishmanial activity based on bioactivity-guided fractionation of ethanolic extracts of leaves and seeds and in vitro activity against promastigotes. In this research the most efficient extracts and fractions were selected for tests on intracellular amastigotes of Leishmania amazonensis. The ethanolic extract of the leaves and dichloromethane and chloroform fractions had IC50 values of 38, 3.9 and 1.2 &amp;#956;g/mL for...
Tipo: Journal article
Palavras-chave: Anti-leishmanial; Azadirachta indica; Cytotoxicity; Leishmaniasis; Leishmania amazonensis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200002

Human intoxication with paralytic shellfish toxins: Clinical parameters and toxin analysis in plasma and urine
Provedor de dados: 48
Autores: GARCÍA,CARLOS; LAGOS,MARCELO; TRUAN,DOMINIQUE; LATTES,KARINNA; VÉJAR,OMAR; CHAMORRO,BEATRIZ; IGLESIAS,VERÓNICA; ANDRINOLO,DARÍO; LAGOS,NÉSTOR.
This study reports the data recorded from four patients intoxicated with shellfish during the summer 2002, after consuming ribbed mussels (Aulacomya ater) with paralytic shellfish toxin contents of 8,066 ± 61.37 mg/100 gr of tissue. Data associated with clinical variables and paralytic shellfish toxins analysis in plasma and urine of the intoxicated patients are shown. For this purpose, the evolution of respiratory frequency, arterial blood pressure and heart rate of the poisoned patients were followed and recorded. The clinical treatment to reach a clinically stable condition and return to normal physiological parameters was a combination of hydration with saline solution supplemented with Dobutamine (vasoactive drug), Furosemide (diuretic) and Ranitidine...
Tipo: Journal article
Palavras-chave: Paralytic shellfish poisoning; PSP human intoxication; Chilean fjords.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200009

Phenolic profiles of nectar and honey of Quillaja saponaria Mol. (Quillajaceae) as potential chemical markers
Provedor de dados: 48
Autores: Montenegro,Gloria; Díaz-Forestier,Javiera; Fredes,Carolina; Rodríguez,Sharon.
Quillaja saponaria Mol. (Quillajaceae) is one of the most important melliferous species in Chile, mainly as a source of monofloral honey. Honey made by A. mellifera presents biological activity against pathogens and antioxidant capacity associated with the presence of phenolic compounds deriving from the nectar, as a result of bee honey foraging. The aim of this study was to identify and quantify the phenolic compounds from the floral nectar of Q. saponaria and the honey made in apiaries in the central zone, and compare the composition of the chromatographic profiles of nectar and honey to known phenolic compounds. The results obtained by HPLC-DAD (high-performance liquid chromatography with diode-array detection) showed a similar profile of phenolic...
Tipo: Journal article
Palavras-chave: Apis mellifera; Chromatographic profile; Floral nectar; Honey; Phenolic compounds; Quillaja saponaria.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200009

Role of nitric oxide during neurogenesis in the olfactory epithelium
Provedor de dados: 48
Autores: SULZ,LORENA; BACIGALUPO,JUAN.
In mammals, neurogenesis continues during adulthood in restricted places of the nervous system, namely the subventricular zone, the dentate gyms and the olfactory epithelium. A dual role of the second messenger nitric oxide has been reported in such places, either promoting or inhibiting proliferation of neuronal precursors depending on the cellular signal implicated. In this review the regulation of adult olfactory epithelium neurogenesis by nitric oxide is discussed
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500002

Factores de impacto de Biological Research: 1998-2004
Provedor de dados: 48
Autores: RAU,JAIME R.
Tipo: Journal article
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200004

Zinc in the prevention of Fe2initiated lipid and protein oxidation
Provedor de dados: 48
Autores: ZAGO,M. PAOLA; VERSTRAETEN,SANDRA V.; OTEIZA,PATRICIA I.
In the present study we characterized the capacity of zinc to protect lipids and proteins from Fe2+-initiated oxidative damage. The effects of zinc on lipid oxidation were investigated in liposomes composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS) at a molar relationship of 60:40 (PC:PS, 60:40). Lipid oxidation was evaluated as the oxidation of cis-parinaric acid or as the formation of 2-thiobarbituric acid-reactive substances (TBARS). Zinc protected liposomes from Fe2+ (2.5-50 muM)-supported lipid oxidation. However, zinc (50 muM) did not prevent the oxidative inactivation of glutamine synthelase and glucose 6-phosphate dehydrogenase when rat brain superntants were oxidized in the presence of 5 muM Fe2+ and 0.5 mM H2O2 .We also...
Tipo: Journal article
Palavras-chave: Aluminum; Catechins; Free radicals; Iron; Lipid peroxidation; Zinc.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200014

Porcine oviduct sperm binding glycoprotein and its deleterious effect on sperm: a mechanism for negative selection of sperm?
Provedor de dados: 48
Autores: Teijeiro,Juan M; Dapino,Dora G; Marini,Patricia E.
In their journey through the oviduct some subpopulations of sperm are preserved in a reservoir, while others are negatively selected. Sperm binding glycoprotein (SBG) is a pig oviductal epithelial cell glycoprotein that produces, under capacitating conditions, acrosome alteration, p97 tyrosine-phosphorylation and reduction of the motility of sperm. In this paper, we show that SBG is accessible at the extracellular surface of the oviductal epithelial cells, supporting a sperm interaction biological role in situ. We analyze the possible dependence of the tyrosine-phosphorylation of p97 on the PKA mechanism, finding that apparently it is not PKA dependent. Also, after SBG treatment the phosphorylated proteins locate mainly at the detached periacrosomal region...
Tipo: Journal article
Palavras-chave: Acrosome alteration; Tyrosine-phosphorylation.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400003

EDUCACIÓN SUPERIOR: DESAFÍOS Y TAREAS
Provedor de dados: 48
Autores: BRUNNER,JOSÉ JOAQUÍN.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100004

Effects of shape variations on the energy metabolism of the sand cricket Gryllus firmus: a geometric morphometric analysis
Provedor de dados: 48
Autores: Nespolo,Roberto F; Sepúlveda,Roger D; Castañeda,Luis E; Roff,Derek A.
Respiration and energy metabolism are key processes in animals, which are severely constrained by the design of physical structures, such as respiratory structures. Insects have very particular respiratory systems, based on gas diffusion across tracheae. Since the efficiency of the tracheal respiratory system is highly dependent on body shape, the pattern of morphological variation during ontogeny could have important metabolic consequences. We studied this problem combining through-flow respirometry and geometric morphometrics in 88 nymphs of the sand cricket, Gryllus firmus. After measuring production in each individual, we took digital photographs and defined eight landmarks for geometric morphometric analysis. The analysis suggested that ontogenic...
Tipo: Journal article
Palavras-chave: Shape variations; Metabolic rate; Morphology; Insect respiration; Orthopter; Sand crickets.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100009

Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
Provedor de dados: 48
Autores: BALDEIRAS,INÊS E; SANTOS,ROSA M; ROSÁRIO,LUÍS M.
Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 mM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50%. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation...
Tipo: Journal article
Palavras-chave: Cytosolic free Ca2+ concentration; 5-HT amperometry; Islet of Langerhans; Protein kinase C; Pulsatile insulin release; Thymeleatoxin.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300014

Bioethical aspects of basic research and medical applications of human stem cells
Provedor de dados: 48
Autores: Santos,Manuel J; Ventura-Juncá,Patricio.
The new discoveries, the extraordinary dynamism in human stem cell (SC) research, and the great expectations of the benefits in clinical treatment of many diseases are on the edge of unparalleled advances in both: 1) the understanding of basic mechanisms of cell differentiation and development and 2) the translation from basic research to new clinical therapies. Human stem cells are obtained from different sources, such as embryo, fetal, and adult tissues, in vitro induction (iPS cells) or transdifferentiation. The evidence that these cells are pluripotent (or multipotent), meaning they have the ability to differentiate into all body tissues or tissues of the same lineage, raises the possibility that they could regenerate diseased or damaged tissue in...
Tipo: Journal article
Palavras-chave: Human Stem cells; Basic research; Bioethics; Clinical trials; Translational research.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300013

Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
Provedor de dados: 48
Autores: MOZDARANI,HOSSEIN; MORADI,SHABNAM Z.
This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration...
Tipo: Journal article
Palavras-chave: Cryopreservation; Vitrification; Mouse embryo; Viability; Chromosome abnormalities.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004

Fluorescent serum and urinary advanced glycoxidation end-products in non- diabetic subjects
Provedor de dados: 48
Autores: DE LA MAZA,MP; BRAVO,A; LEIVA,L; GATTÁS,V; PETERMANN,M; GARRIDO,F; BUNOUT,D; HIRSCH,S; BARRERA,G; FERNÁNDEZ,M.
Introduction: Advanced glycoxidation end-products (AGEs) are involved in age-related conditions and diabetic complications. Diet intake contributes to their circulating concentrations. Aim: To measure serum and urinary AGEs in non-diabetic volunteers and relate their concentration to body composition, blood chemistry and dietary ingestión. Methods: We studied 41 adult men (31 middle-aged adults and 10 elderly). A nutritional assessment including a dietary recall designed for detection of AGE ingestión (specifically carboxymethyl-lysine(CML)), and anthropometric measurements were performed. Also serum lipoproteins, insulin, glucose, leptin and C reactive protein (CRP). AGEs were measured in serum and urine samples using size exclusion chromatography and...
Tipo: Journal article
Palavras-chave: Advanced glycation end-products; Fluorescent AGEs; Glycoxidation.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200011

Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
Provedor de dados: 48
Autores: CHÁVEZ,RENATO; ALMARZA,CAROLINA; SCHACHTER,KAREN; PEIRANO,ALESSANDRA; BULL,PAULINA; EYZAGUIRRE,JAIME.
Penicillium purpurogenum produces several endoxylanases, two of which (XynA and XynB) have been purified and characterized. XynB has been sequenced, and it belongs to glycosyl hydrolase family 11. In this publication we report the structure of the xynA gene. The amino terminal sequence of the protein was determined and this allowed the design of oligonucleotides for use in polymerase chain reactions. Different polymerase chain reaction strategies were used to amplify and sequence the entire cDNA and the gene. The gene has an open reading frame of 1450 base pairs, including 8 introns with an average length of 56 base pairs each. Only one copy of this gene is present in the P. purpurogenum genome as shown by Southern blot. The gene encodes a protein of 329...
Tipo: Journal article
Palavras-chave: Family 10 endoxylanases; Gene structure; Penicillium purpurogenum.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300009

DMT1: Which metals does it transport?
Provedor de dados: 48
Autores: GARRICK,MICHAEL D; SINGLETON,STEVEN T; VARGAS,FARIDA; KUO,H-C; ZHAO,LIN; KNÖPFEL,MARTIN; DAVIDSON,TODD; COSTA,MAX; PARADKAR,PRASAD; ROTH,JEROME A; GARRICK,LAURA M.
DMT1 _ Divalent Metal (Ion) Transporter 1 or SLC11A2/DCT1/Nramp2 _ transports Fe2+ into the duodenum and out of the endosome during the transferrin cycle. DMT1 also is important in non-transferrin bound iron uptake. It plays similar roles in Mn2+ trafficking. Voltage clamping showed that six other metals evoked currents, but it is unclear if these metals are substrates for DMT1. This report summarizes progress on which metals DMT1 transports, focusing on results from the authors' labs. We recently cloned 1A/+IRE and 2/-IRE DMT1 isoforms to generate HEK293 cell lines that express them in a tetracycline-inducible fashion, then compared induced expression to uninduced expression and to endogenous DMT1 expression. Induced expression increases ~50x over...
Tipo: Journal article
Palavras-chave: Metal ion transport; DCT1; Nramp2 or SLC11A2; Expression assay; Competition between metal ions.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100009

A review on plant importance, biotechnological aspects, and cultivation challenges of jojoba plant
Provedor de dados: 48
Autores: Al-Obaidi,Jameel R.; Halabi,Mohammed Farouq; AlKhalifah,Nasser S.; Asanar,Shanavaskhan; Al-Soqeer,Abdulrahman A.; Attia,M. F.
Abstract Jojoba is considered a promising oil crop and is cultivated for diverse purposes in many countries. The jojoba seed produces unique high-quality oil with a wide range of applications such as medical and industrial-related products. The plant also has potential value in combatting desertification and land degradation in dry and semi-dry areas. Although the plant is known for its high-temperature and high-salinity tolerance growth ability, issues such as its male-biased ratio, relatively late flowering and seed production time hamper the cultivation of this plant. The development of efficient biotechnological platforms for better cultivation and an improved production cycle is a necessity for farmers cultivating the plant. In the last 20 years, many...
Tipo: Journal article
Palavras-chave: Simmondsia chinensis; Oil crop; Combat desertification; Desert plants.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100503

Plant Science research productivity in Chile during the past 20 years
Provedor de dados: 48
Autores: KRAUSKOPF,ERWIN.
The purpose of this study was to carry out a bibliometric analysis of the articles published in Chile during 1987-2006 that were categorized into the Plant Science discipline by ISI. The data was extracted from the Web of Science datábase, obtaining a total of 911 articles for analysis. Almost half of the articles were the outcome of an international collaboration, the United States being the country with more collaborative links with Chile within this discipline. A list was made with the 10 most-cited articles and 10 most frequently used journals. The analysis showed that almost 50% of the articles were concentrated in 10 different journals. The number of articles produced each year revealed a steady growth in scientific production since 1987. This is...
Tipo: Journal article
Palavras-chave: Bibliometric; Plant science; Productivity; Epistemometric.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200002

uvsZ1 mutation shows epistatic relations with uvsD153 and uvsJ1 mutations without any involvement with checkpoint control in Aspergillus nidulans
Provedor de dados: 48
Autores: BAPTISTA,FRANCIELLE; AVEZUM ALVES DE CASTRO-PRADO,MARIALBA.
The participation of the recently described uvsZ1 mutation in checkpoint control and the identification of epistatic relations between uvsZ1 mutation and uvsD153 and uvsJ1 mutations are provided. The effect of mutation uvsZ1 in mitotic exchanges into paba-bi (chromosome I) and cho-nic (chromosome VII) genetic intervals has also been evaluated. The mutation uvsZ1 was epistatic with regard to uvsD153 and uvsJ1 mutations, with no involvement with checkpoint control. In contrast to mutations in UvsB and UvsF groups, the uvsZ1 mutation failed to cause any changes in the frequencies of mitotic crossing-over. The distinct phenotypic traits given by mutation uvsZ1 suggest the presence of complex interactions among the different DNA repair pathways. Interaction may...
Tipo: Journal article
Palavras-chave: Checkpoint pathways; DNA repair; Uvs mutations.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300018

MiR-320 inhibits the growth of glioma cells through downregulating PBX3
Provedor de dados: 48
Autores: Pan,Cuicui; Gao,Hua; Zheng,Ni; Gao,Qi; Si,Yuanquan; Zhao,Yueran.
Abstract  Background  MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3.  Methods  Twenty-four human glioma and paired adjacent nontumorous tissues were collected for determination of miR-320 and PBX3 expression using RT-qPCR and western blot assays. Luciferase reporter assay was performed to verify the interaction between miR-320 and its targeting sequence in...
Tipo: Journal article
Palavras-chave: Glioma; Luciferase reporter assay; MiR-320; PBX3.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100224

The vitality of death discussions: Comments on Bacigalupo et al., 'The debate about death: an imperishable discussion?'
Provedor de dados: 48
Autores: SERANI-MERLO,ALEJANDRO.
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500015

Cop-like operon: Structure and organization in species of the Lactobacillale order
Provedor de dados: 48
Autores: REYES,ANGÉLICA; LEIVA,ANDREA; CAMBIAZO,VERÓNICA; MÉNDEZ,MARCO A; GONZÁLEZ,MAURICIO.
Copper is an essential and toxic trace metal for bacteria and, therefore, must be tightly regulated in the cell. Enterococcus hirae is a broadly studied model for copper homeostasis. The intracellular copper levels in E. hirae are regulated by the cop operon, which is formed by four genes: copA and copB that encode ATPases for influx and efflux of copper, respectively; copZ that encodes a copper chaperone; and copY, a copper responsive repressor. Since the complete genome sequence for E. hirae is not available, it is possible that other genes may encode proteins involved in copper homeostasis. Here, we identified a cop-like operon in nine species of Lactobacillale order with a known genome sequence. All of them always encoded a CopY-like repressor and a...
Tipo: Journal article
Palavras-chave: Cop operon; CopY binding site; Copper homeostasis; Enterococcus hirae; Lactobacillale order.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100010

DAVID YUDILEVICH LEVY, CAMINANTE Y VISIONARIO (1930-2006)
Provedor de dados: 48
Autores: BACIGALUPO,JUAN; DEVÉS,ROSA.
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200001

XLVIII REUNIÓN ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CHILE XII REUNION ANUAL DE LA SOCIEDAD DE ECOLOGÍA DE CHILE XXVII CONGRESO CHILENO DE MICROBIOLOGÍA
Provedor de dados: 48
Tipo: Journal article
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200020

A study on &amp;#946;-defensin-2 and histatin-5 as a diagnostic marker of early childhood caries progression
Provedor de dados: 48
Autores: Jurczak,Anna; Ko&amp;#347;cielniak,Dorota; Papie&amp;#380;,Monika; Vyhouskaya,Palina; Krzy&amp;#347;ciak,Wirginia.
BACKGROUND: Recently, a continuous growth of interest has been observed in antimicrobial peptides (AMPs) in the light of an alarming increase in resistance of bacteria and fungi against antibiotics. AMPs are used as biomarkers in diagnosis and monitoring of oral cavity pathologies. Therefore, the determination of specific protein profiles in children diagnosed with early childhood caries (ECC) might be a basis for effective screening tests and specialized examinations which may enable progression of disease METHODS: The objective of the studies was to determine the role of histatin-5 and &amp;#946;-defensing-2 as a diagnostic marker of early childhood caries progression. In this work, results of concentration determination of two salivary proteins...
Tipo: Journal article
Palavras-chave: &amp;#946; -defensin-2 Histatin-5 Early childhood caries.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100061

Wheel-running and rest activity pattern interaction in two octodontids (Octodon degus, Octodon bridgesi)
Provedor de dados: 48
Autores: OCAMPO-GARCÉS,ADRIÁN; HERNÁNDEZ,FELIPE; MENA,WILSON; PALACIOS,ADRIÁN G.
Wheel-running and other non-photic stimuli influence the rest-activity pattern of diurnal and nocturnal mammals. A day to night inversion of phase preference of activity was described among Octodon degus, when exposed to ad-libitum wheel running. We have studied the rest-activity pattern response in presence of ad libitum wheel-running in wild-captured male individuals from two species of genus Octodon: O. degus (n=9, crepuscular-diurnal) and O. bridgesi (n=3, nocturnal). After two months of habituation to laboratory conditions, recordings were performed in isolation chambers under a 12:12 light-dark schedule with or without access to a running wheel. Actograms were constructed from data obtained by an automated acquisition system. O. bridgesi were also...
Tipo: Journal article
Palavras-chave: Circadian rhythms; Masking; Octodon degus; Octodon bridgesi; Wheel-running.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200019

Phytochemical and toxicological investigations of crude methanolic extracts, subsequent fractions and crude saponins of Isodon rugosus
Provedor de dados: 48
Autores: Sadiq,Anwar Zeb Abdul; Ullah,Farhat; Ayaz,Sajjad Ahmad Muhammad.
BACKGROUND: Isodon rugosus is used traditionally in the management of hypertension, rheumatism, tooth-ache and pyrexia. Present study was arranged to investigate I. rugosus for phytoconstituents, phytotoxic and cytotoxic activities to explore its toxicological, pharmacological potentials and to rationalize its ethnomedicinal uses. Briefly, qualitative phytochemical analysis of the plant extracts were carried out for the existence of alkaloids, flavonoids, saponins, oils, glycosides, anthraquinones, terpenoids, sterols and tannins. Plant crude methanolic extract (Ir.Cr), its subsequent fractions; n-hexane (Ir.Hex), chloroform (Ir.Chf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq) and saponins (Ir.Sp) in different concentrations were tested for phytotoxic and...
Tipo: Journal article
Palavras-chave: Isodon rugosus; Cytotoxicity; Phytotoxicity; Brine shrimps.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100051

Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
Provedor de dados: 48
Autores: Orozco-Ibarra,Marisol; Muñoz-Sánchez,Jorge; Zavala-Medina,Martín E; Pineda,Benjamín; Magaña-Maldonado,Roxana; Vázquez-Contreras,Edgar; Maldonado,Perla D; Pedraza-Chaverri,José; Chánez-Cárdenas,María Elena.
BACKGROUND: Aged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the &amp;#945; subunit of hypoxia inducible factor (HIF-&amp;#921;&amp;#945;) and up-regulation of HIF-1a-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells RESULTS: We found that CoCl2 induced the stabilization of HIF-1a and its...
Tipo: Journal article
Palavras-chave: Aged garlic extract; Chemical hypoxia; Cobalt chloride; Hypoxia-inducible factor 1; PC12 cells; S-allylcysteine.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100007

Are good ideas enough?: The impact of socio-economic and regulatory factors on GMO commercialisation
Provedor de dados: 48
Autores: Vàzquez-Salat,Núria.
In recent years scientific literature has seen an increase in publications describing new transgenic applications. Although technically-sound, these promising developments might not necessarily translate into products available to the consumer. This article highlights the impact of external factors on the commercial viability of Genetically Modified (GM) animals in the pharmaceutical and food sectors. Through the division of the production chain into three Policy Domains -Science, Market and Public- I present an overview of the broad range of regulatory and socio-economic components that impacts on the path towards commercialisation of GM animals. To further illustrate the unique combination of forces that influence each application, I provide an in-depth...
Tipo: Journal article
Palavras-chave: Genetically modified organisms; GM animals; Policy domains; Governance; Socio-economic factors.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400002

Description of the immatures of Linepithema humile Mayr (Hymenoptera: Formicidae)
Provedor de dados: 48
Autores: SOLIS,DANIEL R; FOX,EDUARDO G. P; ROSSI,MÔNICA L; BUENO,ODAIR C.
Linepithema humile Mayr is an ant species originally native to South America that has been spread accidently throughout the globe through international trade. It is a serious urban and crop pest. Despite its economic importance, little is known about the larvae of this species apart from a brief description based on a few specimens. The present investigation is aimed at describing every immature stage of L. humile. Three larval instars were determined through the frequency distribution of the maximum width of head capsules from a sample of 525 larvae. The morphological descriptions were based on 150 eggs, 70 larvae, and 90 pupae examined by light and scanning electron microscopy. Some morphological characteristics reported to be typical of Linepithema Mayr...
Tipo: Journal article
Palavras-chave: Argentine ant; Dolichoderinae; Dolichoderini; Iridomyrmex humilis; Larval instars; Tramp species.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100004

Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture
Provedor de dados: 48
Autores: Chen,Peng; Wang,Yuxia; Yan,Lei; Wang,Yiqing; Li,Suyue; Yan,Xiaojuan; Wang,Ningbo; Liang,Ning; Li,Hongyu.
BACKGROUND: The development of clean or novel alternative energy has become a global trend that will shape the future of energy. In the present study, 3 microbial strains with different oxygen requirements, including Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D, were used to construct a hydrogen production system that was composed of a mixed aerobic-facultative anaerobic-anaerobic consortium. The effects of metal ions, organic acids and carbohydrate substrates on this system were analyzed and compared using electrochemical and kinetic assays. It was then tested using small-scale experiments to evaluate its ability to convert starch in 5 L of organic wastewater into hydrogen. For the one-step...
Tipo: Journal article
Palavras-chave: Renewable Energy; Biohydrogen; Microbial consortium; Hydrogen.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100024

UN BALANCE EDITORIAL DE BIOLOGICAL RESEARCH
Provedor de dados: 48
Autores: KRAUSKOPF,MANUEL.
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300001

Protective effect of bixin on carbon tetrachloride-induced hepatotoxicity in rats
Provedor de dados: 48
Autores: Moreira,Priscila R; Maioli,Marcos A; Medeiros,Hyllana CD; Guelfi,Marieli; Pereira,Flávia TV; Mingatto,Fábio E.
BACKGROUND: The liver is an important organ for its ability to transform xenobiotics, making the liver tissue a prime target for toxic substances. The carotenoid bixin present in annatto is an antioxidant that can protect cells and tissues against the deleterious effects of free radicals. In this study, we evaluated the protective effect of bixin on liver damage induced by carbon tetrachloride (CCl4) in rats. RESULTS: The animals were divided into four groups with six rats in each group. CCl4 (0.125 mL kg-1 body wt.) was injected intraperitoneally, and bixin (5.0 mg kg-1 body wt.) was given by gavage 7 days before the CCl4 injection. Bixin prevented the liver damage caused by CCl4, as noted by the significant decrease in serum aminotransferases release....
Tipo: Journal article
Palavras-chave: Bixin; Carbon tetrachloride; Hepatotoxicity; Oxidative stress; Protective activity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100072

Antiulcer activity of ethanolic extract of Encholirium spectabile Mart. ex Schult &amp;amp; Schult f. (Bromeliaceae) in rodents
Provedor de dados: 48
Autores: Moraes de Carvalho,Katharinne I; Fernandes,Hélio B; Frota Machado,Flávia D; Oliveira,Irisdalva S; Oliveira,Francisco A; Nunes,Paulo Humberto M; Lima,Julianeli T; Silva Almeida,Jackson R G; Meneses Oliveira,Rita C.
This study evaluated the antiulcer activity of an ethanolic extract of Encholirium spectabile (ES-EtOH) by using different standard experimental models of induced acute gastric ulceration. ES-EtOH (100 mg/kg p.o) protected the gastric mucosa against ulceration that was induced by absolute ethanol (53%), ethanol/HCl (75 %), ibuprofen (52 %) and ischemia/reperfusion (43 %). It also restored catalase activity and non-protein sulfhydryl group concentration in the gastric wall of mice that had been treated with ethanol. The pre-treatment of mice with N-nitro-L-arginine (70 mg/kg i.p.) abolished the protective activity of ES-EtOH, which indicates that prostaglandins, antioxidant compounds and nitric oxide synthase activity are involved in the gastroprotective...
Tipo: Journal article
Palavras-chave: Antioxidant; Antiulcer activity; Encholirium spectabile; Bromeliaceae.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400011

Growth of in vitro Fusarium oxysporum f. sp. niveum in chemically defined media amended with gallic acid
Provedor de dados: 48
Autores: WU,HONG-SHENG; WANG,YANG; ZHANG,CHAO-YONG; BAO,WEI; LING,NING; LIU,DONG-YANG; SHEN,QI-RONG.
Gallic acid was artificially added to the media to grow Fusarium oxysporum f.sp.niveum to investigate its effect on the pathogenic fungus. Results indicate that gallic acid inhibited the growth of F. oxysporum f.sp.niveum. The colony diameter, the conidia germinating rate and the conidia yield were reduced by 5.7-22.9%%, 35.8-55.6% and 38.9-62.2% respectively. However, the virulence factors by the fungus were stimulated. The activity of pectinase, proteinase and cellulase increased by 12.3-627.8%, 11.8-41.2% and 0.5-325.0% respectively, while the activity of amylase increased slightly. The results suggest that gallic acid repressed growth but facilitated the relative pathogenicity of invading pathogens.
Tipo: Journal article
Palavras-chave: Allelopathy; Gallic acid; Fusarium oxysporum f. sp. niveum; Plant-microbe interaction; Virulent factors.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300004

Species composition and seasonal succession of saprophagous calliphorids in a rural area of Córdoba: Argentina
Provedor de dados: 48
Autores: BATTAN HORENSTEIN,MOIRA; LINHARES,ARICIO X; ROSSO,BEATRIZ; GARCÍA,MARÍA D.
During 2004, four experiments were carried out, one each season, in order to determine the species composition and seasonal dynamics of Calliphoridae in a rural area of Córdoba (Argentina). Two pigs (Sus seroja L.), weighing approximately 8 Kg each, were used in each experiment. They were killed with a blow to the head and immediately placed in a variant of the Schoenly et al. (1991) trap. One pig was exposed in the shade and the other one under direct sunlight. The insect fauna was collected daily during the four first weeks and every two or three days thereafter. A total of 16.609 adults of Calliphoridae were collected, and later on identified as one of the following seven species: Chrysomya albiceps (Wiedemann), Chrysomya megacephala (Fabricius),...
Tipo: Journal article
Palavras-chave: Calliphorids; Necrophages; Seasonal dynamics; Succession.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200007

Growth, delayed fluorescence and pigment composition of four Prorocentrum minimum strains growing at two salinities
Provedor de dados: 48
Autores: BERDEN-ZRIMEC,MAJA; FLANDER-PUTRLE,VESNA; DRINOVEC,LUKA; ZRIMEC,ALEXIS; MONTI,MARINA.
Prorocentrum minimum is a potentially harmful and widely distributed marine dinoflagellate. Several P. minimum strains have already been studied, showing phylogenetical relations of strains isolated from the same geographical regions. Similarity among the strains was further examined on the basis of their physiology. Pigment composition and concentration, as well as delayed fluorescence (DF) decay kinetics and intensity, were measured in four P. minimum strains isolated from the Baltic and Adriatic Seas. The strains were grown at two salinities characteristic of the Baltic (8 PSU) and North Adriatic Seas (32 PSU). Strain differences in DF decay kinetics and growth did not always follow their genetic relations. While two strains showed similarities to the...
Tipo: Journal article
Palavras-chave: Adriatic Sea; Baltic Sea; Delayed fluorescence; HPLC; Prorocentrum minimum; Strains.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100003

Effects of crowding and sex on fecal cortisol levels of captive forest musk deer
Provedor de dados: 48
Autores: He,Lan; Wang,Wen-Xia; Li,Lin-Hai; Liu,Bao-Qing; Liu,Gang; Liu,Shu-Qiang; Qi,Lei; Hu,De-Fu.
BACKGROUND: Restricted space and close contact with conspecifics in captivity may be stressful for musk deer, as they are highly territorial and solitary in the wild. So we tested the effects of crowding on stress of forest musk deer (Moschus berezovskii) in heterosexual groups, using fecal cortisol analysis as a non-invasive method. 32 healthy adults during non-breeding seasons were chose as our experimental objects. Group 1 was defined as higher crowding condition, with 10-15 m²/deer (6 enclosures, 10&amp;#9792; and 6&amp;#9794;); group 2 was defined as lower crowding condition, with 23-33 m²/deer (6 enclosures, 10&amp;#9792; and 6&amp;#9794;). Every enclosure contained 1 male and 3 female. These patterns had been existed for years. RESULTS: The results...
Tipo: Journal article
Palavras-chave: Captivity; M. berezovskii; Fecal cortisol; Chronic stress; Crowding.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100071

Red Wine administration to Apolipoprotein E-deficient Mice reduces their Macrophage-derived Extracellular Matrix Atherogenic Properties
Provedor de dados: 48
Autores: KAPLAN,MARIELLE; AVIRAM,MICHAEL.
Proteoglycans (PGs) from the arterial extracellular matrix (ECM) contribute to the trapping of LDL and oxidized LDL (Ox-LDL) in the arterial wall, a phenomenon called "lipoprotein retention". Moreover, we have shown that subsequent to their binding to the matrix, LDL and Ox-LDL are taken up by macrophages. Oxidative stress significantly increases macrophage secretion of ECM-PGs, lipoprotein binding to the ECM and the uptake of ECM-retained lipoproteins by macrophages. The aim of the present study was to determine whether red wine administration to atherosclerotic mice would affect their peritoneal macrophage-derived extracellular matrix properties, such as the glycosaminoglycan content and the ability to bind LDL. In addition, we questioned the ability of...
Tipo: Journal article
Palavras-chave: Antioxidants; Atherosclerosis; Extracellular matrix; Macrophages; Polyphenols; Proteoglycans; Red wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200010

Ciencia y Educación: Una tarea pendiente
Provedor de dados: 48
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100001

Redox regulation of RyR-mediated Ca2+ release in muscle and neurons
Provedor de dados: 48
Autores: HIDALGO,CECILIA; BULL,RICARDO; BEHRENS,M. ISABEL; DONOSO,PAULINA.
Changes in the redox state of the intracellular ryanodine receptor/Ca2+ release channels of skeletal and cardiac muscle or brain cortex neurons affect their activity. In particular, agents that oxidize or alkylate free SH residues of the channel protein strongly enhance Ca2+-induced Ca2+ release, whereas reducing agents have the opposite effects. We will discuss here how modifications of highly reactive cysteine residues by endogenous redox agents or cellular redox state influence RyR channel activation by Ca2+ and ATP or inhibition by Mg2+. Possible physiological and pathological implications of these results on cellular Ca2+ signaling will be addressed as well.
Tipo: Journal article
Palavras-chave: Redox state; Ryanodine receptors; Sarcoplasmic/endoplasmic reticulum; Calcium release; S-nitrosylation; S-glutathionylation.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400007

On living beings and metaphysical options
Provedor de dados: 48
Autores: SERANI-MERLO,A.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100003

Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
Provedor de dados: 48
Autores: Santana,Andréia T; Guelfi,Marieli; Medeiros,Hyllana C D; Tavares,Marco A; Bizerra,Paulo F V; Mingatto,Fábio E.
BACKGROUND: Gossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanisms of reproductive toxicity of gossypol in rats and the protective effects of vitamin E. Forty Wistar rats were used, divided into four experimental groups (n = 10): DMSO/ saline + corn oil; DMSO/saline + vitamin E; gossypol + corn oil; and gossypol + vitamin E. RESULTS: Fertility was significantly reduced in male rats treated with gossypol in that a significant decrease in epididy-mal sperm count was observed (P &lt; 0.05) and the number of offspring was...
Tipo: Journal article
Palavras-chave: Gossypol; Fertility; Oxidative stress; Bioenergetics; Antioxidant system; Vitamin E.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100043

Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols
Provedor de dados: 48
Autores: Naito,Yuji; Shimozawa,Makoto; Manabe,Hiroki; Kuroda,Masaaki; Tomatsuri,Naoya; Uchiyama,Kazuhiko; Takagi,Tomohisa; Yoshida,Norimasa; Yoshikawa,Toshikazu.
Red wine polyphenolic compounds have been demonstrated to possess antioxidant properties, and several studies have suggested that they might constitute a relevant dietary factor in the protection from coronary heart disease. The aim of the present study is to examine whether red wine extracts (RWE) can ameliorate oxysterol-induced endothelial response, and whether inhibition of adhesion molecule expression is involved in monocyte adhesion to endothelial cells. Surface expression and mRNA levels of adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1) were determined by ELISA and RT-PCR performed on human aortic endothelial cells (HAEC) monolayers stimulated with 7b-hydroxycholesterol or 25-hydroxycholesterol....
Tipo: Journal article
Palavras-chave: Adhesion molecule; Monocyte-endothelial cell interaction; Oxysterol; Red wine extract.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200009

A spleen tyrosine kinase inhibitor attenuates the proliferation and migration of vascular smooth muscle cells
Provedor de dados: 48
Autores: Seo,Hyang&amp;#8209;Hee; Kim,Sang Woo; Lee,Chang Youn; Lim,Kyu Hee; Lee,Jiyun; Choi,Eunhyun; Lim,Soyeon; Lee,Seahyoung; Hwang,Ki&amp;#8209;Chul.
Abstract  Background  Pathologic vascular smooth muscle cell (VSMC) proliferation and migration after vascular injury promotes the development of occlusive vascular disease. Therefore, an effective chemical agent to suppress aberrant proliferation and migration of VSMCs can be a potential therapeutic modality for occlusive vascular disease such as atherosclerosis and restenosis. To find an anti-proliferative chemical agent for VSMCs, we screened an in-house small molecule library, and the selected small molecule was further validated for its anti-proliferative effect on VSMCs using multiple approaches, such as cell proliferation assays, wound healing assays, transwell migration assays, and ex vivo aortic ring assay.  Results  Among 43 initially screened...
Tipo: Journal article
Palavras-chave: Syk kinase inhibitor; BAY61-3606 VSMC; Proliferation; Migration.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100403

XXXVIII REUNIÓN ANUAL GENÉTICA DE CHILE
Provedor de dados: 48
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000400003

Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin
Provedor de dados: 48
Autores: Vargas,Álvaro; Bustos-Obregón,Eduardo; Hartley,Ricardo.
Hypobaric hypoxia is of interest due to an increase of human populations working at high altitude. Testicular damage is related to the physiological response (neoangiogenesis) to increased intrascrotal blood flow as temperature rises. Hypoxia is a stress factor with overproduction of reactive oxygen species (ROS). The effect of hypoxia in mice reproductive parameters is analyzed. Animals were exposed to simulated hypoxia of 4,200 meters above sea level (m.a.s.l.) in a chamber for 33.2 days, both to continuous (HH) or intermittent hypoxia (HI) with an intermittency period of 4 days hypoxia /4 days normoxia (500 m.a.s.l.). The anti-inflammatory drug Ibuprofen was administered to a group of mice to control vasodilation and increased blood flow. Melatonin was...
Tipo: Journal article
Palavras-chave: Epididymal sperm; Mouse; Ibuprofen; Melatonin.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200008

Recent advances in chromaffin cell biology: Summing up the last International Symposium on Chromaffin Cell Biology
Provedor de dados: 48
Autores: CÁRDENAS,ANA M.
The International Symposium on Chromaffin Cell Biology (ISCCB) brings together a group of approximately 150 scientists from around the world who meet every 2 years to discuss recent advances in our understanding of biogenesis and motion of secretory vesicles, synthesis, storage and release of secreted products (catecholamines, chromogranins, ATP), and mechanisms involving the excitation-secretion coupling, membrane ion channels, intracellular calcium homeostasis and exocytosis. The development of new technologies that allow an accurate measurement of catecholamines, vesicle motion, exocytosis, etc. are also analyzed. The 12th ISCCB, organized by Ricardo Borges, took place on September 20-26, 2003, in La Palma, Canary Islands, Spain. In this article we...
Tipo: Journal article
Palavras-chave: Chromaffin cells; Calcium channels; Nicotine receptors; Exocytosis; Chromaffin granules; Chromogranins.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100002

The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells
Provedor de dados: 48
Autores: CARBALLO,JESÚS A; PINCHEIRA,JUANA; DE LA TORRE,CONSUELO.
Root growth, G2 length, and the frequency of aberrant mitoses and apoptotic nuclei were recorded after a single X-ray irradiation, ranging from 2.5 to 40 Gy, in Allium cepa L. root meristematic cells. After 72 h of recovery, root growth was reduced in a dose-dependent manner from 10 to 40 Gy, but not at 2.5 or 5 Gy doses. Flow cytometry plus TUNEL (TdT-mediated dUTP nick end labeling) showed that activation of apoptosis occurred only after 20 and 40 Gy of X-rays. Nevertheless, irrespective of the radiation dose, conventional flow cytometry showed that cells accumulated in G2 (4C DNA content). Simultaneously, the mitotic index fell, though a mitotic wave appeared later. Cell accumulation in G2 was transient and partially reversed by caffeine, thus it was...
Tipo: Journal article
Palavras-chave: Apoptosis; Checkpoint adaptation; G2 DNA damage checkpoint; Genome instability; X-rays.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200015

Tridax procumbens flavonoids: a prospective bioactive compound increased osteoblast differentiation and trabecular bone formation
Provedor de dados: 48
Autores: Mamun,Md. Abdullah Al; Hosen,Mohammad Jakir; Khatun,Amina; Alam,M. Masihul; Al-Bari,Md. Abdul Alim.
Abstract  Background  The Tridax procumbens extracts (TPE) are known for their ethno-medicinal properties to increase osteogenic functioning in mesenchymal stem cells. Recently, we found that the T. procumbens flavonoids (TPF) significantly suppressed the RANKL-induced osteoclasts differentiation and bone resorption. The TPF also promoted osteoblasts differentiation and bone formation demonstrated by increasing bone formation markers in cultured mouse primary osteoblasts. However, the effects of the TPF on in vivo bone formation remain unclear. In this study, we investigated the effects of the TPF on in vivo bone formation, injected the TPF (20 mg/kg) twice a day in the low calcium diet mice and killed them after 21 day. Radiographic and histomorphometric...
Tipo: Journal article
Palavras-chave: Alkaline phosphatase; Bone formation; Osteoblast differentiation; Tridax procumbens flavonoids; Trabecular bone.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100221

UNA CONVERSACION CON EL DR. MARIO LUXORO MARIANI: PREMIO NACIONAL DE CIENCIAS NATURALES, AÑO 2000
Provedor de dados: 48
Autores: HIDALGO,CECILIA.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200002

Acidobacteria appear to dominate the microbiome of two sympatric Caribbean Sponges and one Zoanthid
Provedor de dados: 48
Autores: O'Connor-Sánchez,Aileen; Rivera-Domínguez,Adán J; De los Santos-Briones,César; López-Aguiar,Lluvia K; Peña-Ramírez,Yuri J; Prieto-Davo,Alejandra.
BACKGROUND: Marine invertebrate-associated microbial communities are interesting examples of complex symbiotic systems and are a potential source of biotechnological products. RESULTS: In this work, pyrosequencing-based assessment from bacterial community structures of sediments, two sponges, and one zoanthid collected in the Mexican Caribbean was performed. The results suggest that the bacterial diversity at the species level is higher in the sediments than in the animal samples. Analysis of bacterial communities' structure showed that about two thirds of the bacterial diversity in all the samples belongs to the phyla Acidobacteria and Proteobacteria. The genus Acidobacteriumappears to dominate the bacterial community in all the samples, reaching almost...
Tipo: Journal article
Palavras-chave: Marine-invertebrate microbiome; 16S rRNA pyrotags; Mexican Caribbean; Sponge-microbiome; Zoanthid-microbiome; Marine sediments-microbiome.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100061

Editorial
Provedor de dados: 48
Autores: LUXORO,MARIO.
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300001

IGF2 expression in blood is not associated with its imprinting status in healthy pregnant Chinese women
Provedor de dados: 48
Autores: Ru-Fei,Gao; Xue-Qing,Liu; Ying-Xiong,Wang; Xue-Mei,Chen; Yu-Bin,Ding; Jun-Lin,He.
Loss of Imprinting (LOI) of IGF2 and over-expressed IGF2 are associated with tumorigenesis. Our previous epidemiological study found a relatively high frequency of IGF2 LOI in healthy mid-gestation pregnant women. The aim of this study is to determine whether the expression of IGF2 is associated with its imprinting status in healthy Chinese pregnant women. The IGF2 imprinting status of 300 pregnant women was analyzed. 20 cases of IGF2 LOI and 20 cases of IGF2 retention of imprinting (ROI) were selected randomly for IGF2 expression analysis. The expression pattern of IGF2 between the group with IGF2 ROI and group with IGF2 LOI in healthy Chinese pregnant women was evaluated by real time PCR and western blot. The result showed no significant differences...
Tipo: Journal article
Palavras-chave: Insulin-like growth factor 2; Loss of imprinting; Retention of imprinting; Pregnancy.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400004

RECIENTE CONGRESO DE LA SOCIEDAD CHILENA DE INMUNOLOGÍA
Provedor de dados: 48
Autores: AGUILLON,JUAN CARLOS.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300002

Activities of different types of Thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals
Provedor de dados: 48
Autores: Jantakee,Kanyaluck; Tragoolpua,Yingmanee.
BACKGROUND: Honey is a natural product obtained from the nectar that is collected from flowers by bees. It has several properties, including those of being food and supplementary diet, and it can be used in cosmetic products. Honey imparts pharmaceutical properties since it has antibacterial and antioxidant activities. The antibacterial and antioxidant activities of Thai honey were investigated in this study. RESULTS: The honey from longan flower (source No. 1) gave the highest activity on MRSA when compared to the other types of honey, with a minimum inhibitory concentration of 12.5% (v/v) and minimum bactericidal concentration of 25% (v/v). Moreover, it was found that MRSA isolate 49 and S. aureus were completely inhibited by the 50% (v/v) longan honey...
Tipo: Journal article
Palavras-chave: Anti-bacterial activity; Pathogenic bacteria; Anti-free radicals; Anti-tyrosinase.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100004

Effect of chlorocholine chlorid on phenolic acids accumulation and polyphenols formation of buckwheat plants
Provedor de dados: 48
Autores: Sytar,Oksana; Borankulova,Asel; Hemmerich,Irene; Rauh,Cornelia; Smetanska,Iryna.
BACKGROUND: Effect of chlorocholine chloride (CCC) on phenolic acids composition and polyphenols accumulation in various anatomical parts (stems, leaves and inflorescences) of common buckwheat (Fagopyrum esculentum Moench) in the early stages of vegetation period were surveyed. RESULTS: Treatment of buckwheat seeds with 2% of CCC has been increased content of total phenolics in the stems, leaves and inflorescences. On analyzing the different parts of buckwheat plants, 9 different phenolic acids - vanilic acid, ferulic acid, trans-ferulic acid, chlorogenic acid, salycilic acid, cinamic acid, p-coumaric acid, p-anisic acid, methoxycinamic acid and catechins were identified. The levels of identified phenolic acids varied not only significantly among the plant...
Tipo: Journal article
Palavras-chave: Chlorocholine chloride; Phenolic acids; Catechins; Buckwheat.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100019

Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
Provedor de dados: 48
Autores: Gan,Zhihua; Han,Kun; Lin,Shuchen; Hu,Haiyan; Shen,Zan; Min,Daliu.
Abstract  Background  Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet.  Methods  The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. A further western blot analysis also demonstrated an aberrant endogenous expression of USP39 in three different osteosarcoma cells. Then lentivirus-mediated short hairpin RNA (shRNA) was designed to silence USP39 in human osteosarcoma cell line U2OS, which is used to test...
Tipo: Journal article
Palavras-chave: Cell cycle; Migration; Osteosarcoma; Proliferation; USP39.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100210

Antioxidant isolated from Schisandra propinqua (Wall.) Baill
Provedor de dados: 48
Autores: HUANG,FENG; XU,LIJIA; GANGGANG,SHI.
Schisandra propinqua (Wall.) Baill.(Schisandraceae) is widely used as a Chinese folk medicine. In this study, activity-guided fractionation of the ethanol extract from the stem of Schisandra propinqua led to the isolation of four extracts. Subsequently, a neolignan 4,4-di(4-hydroxy-3-methoxyphenly)-2,3-dimethylbutanol was isolated from the EtOAc part of the stem of Schisandra propinqua, the free radical scavenging activities of which were researched in vitro. The present work demonstrated that extracts and pure compound possessed scavenging activities to DPPH, superoxide anions and hydroxy radical, and could depress lipid peroxidation reaction induced by oxygen radical produced by the Fe2+/cysteine system in vitro. This suggests that the traditional...
Tipo: Journal article
Palavras-chave: Antioxidant; Free radical; Lipid peroxidation; Schisandra propinqua.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300009

Protective effect of Cassia fistula Linn. on diethylnitrosamine induced hepatocellular damage and oxidative stress in ethanol pretreated rats
Provedor de dados: 48
Autores: PRADEEP,KANNAMPALLI; RAJ MOHAN,CHANDRASEKARAN VICTOR; GOBIANAND,KUPANNAN; KARTHIKEYAN,SIVANESAN.
Diethylnitrosamine (DEN), found in many commonly consumed foods, is widely reported to induce cancer in animals and humans. The aim of the present study was to investigate the hepatoprotective and antioxidant activities of the leaf extract of the medicinal plant Cassia fistula Linn. against diethylnitrosamine induced liver injury in ethanol pretreated rats. Albino Wistar rats, pretreated with ethanol for 15 days, were administered a single dose of DEN. Thirty days after DEN administration, hepatotocellular damage was observed histologically, along with elevated levels of serum AST, ALT, ALP, LDH, &amp;#947;-GT and bilirubin and a simultaneous fall in the levels of the marker enzymes in the liver tissue. Liver oxidative stress was confirmed by elevated...
Tipo: Journal article
Palavras-chave: Antioxidants; Cassia fistula; Diethylnitrosamine; Hepatoprotective; Lipid peroxidation; Silymarin.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100013

Developmental regulation of the expression of sodium currents in Xenopus primary neurons
Provedor de dados: 48
Autores: OLGUÍN,PATRICIO; ARMISEN,RICARDO; KUKULJAN,MANUEL.
The electrophysiological properties of neurons are determined by the expression of defined complements of ion channels. Nonetheless, the regulation mechanisms of the expression of neuronal ion channels are poorly understood, due in part to the diversity of neuron subtypes. We explored the expression of voltage-gated currents of Xenopus primary spinal neurons unequivocally identified by means of single-cell RT-PCR. We found that identified spinal neurons exhibit heterogeneity in the temporal appearance of voltage-gated currents. Nevertheless, all neurons progress to similar functional phenotypes. A physiological feature is the onset and increase of the expression of sodium currents. To understand the mechanisms underlying this process, we studied the effect...
Tipo: Journal article
Palavras-chave: Excitability; REST/NRSF; Neuronal differentiation; Single-cell RT-PCR transcription.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300010

Polytypic and polymorphic cytogenetic variations in the widespread anuran Physalaemus cuvieri (Anura, Leiuperidae) with emphasis on nucleolar organizing regions
Provedor de dados: 48
Autores: QUINDERÉ,YEDA R. S. D; LOURENÇO,LUCIANA B; ANDRADE,GILDA V; TOMATIS,CRISTIAN; BALDO,DIEGO; RECCO-PIMENTEL,SHIRLEI M.
We investigated the NOR distribution in ten populations of Physalaemus cuvieri from different regions of Brazil and Argentina. A high variability in NOR pattern was observed and provided a useful tool in grouping several populations. The specimens from the state of Tocantins, northern Brazil, could easily be distinguished from all the other analyzed populations, since its karyotype presented NORs in the chromosome pairs 1,3,4 and 10 (and sometimes also in chromosome 5), and several pericentromeric C-bands. A NOR-site in chromosome 9 characterized three populations from the northeastern region of Brazil. Interestingly, the P. cuvieri populations located in opposite extremes of the geographic distribution had, as a fixed condition, the presence of NORs in 8q...
Tipo: Journal article
Palavras-chave: Anura; Chromosome; Cytogenetics; NOR; Physalaemus; Polymorphic variations; Polytypic variations.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100008

In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
Provedor de dados: 48
Autores: LÓPEZ,MAURICIO; FIGUEROA,JAIME; KAUSEL,GUDRUN; VERA,MARÍA INÉS; KRAUSKOPF,MANUEL.
We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter- acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the...
Tipo: Journal article
Palavras-chave: Acclimatization; In situ hybridization; Somatolactin expression; Pituitary; Carp; Fish.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100010

Effects of the seed predator Acanthoscelides schrankiae on viability of its host plant Mimosa bimucronata
Provedor de dados: 48
Autores: TOMAZ,CAMILA A; KESTRING,DÉBORA; ROSSI,MARCELO N.
Seeds of Mimosa bimucronata are heavily infested (pre-dispersal predation) by the bruchid beetle Acanthoscelides schrankiae in Brazil. In this study, firstly we set up experiments to assess seed germination under seven and six different light and temperature regimes, respectively, and then we evaluated the ability of seeds to germinate after predation. We tested the hypothesis that the non-predated seeds from infested fruits may respond differently when set for germination than those seeds of non-infested fruits. We also hypothesized that predation may increase the production of unviable seeds. Seeds under 18 hours of light presented the highest percentage of germination, and the alternating temperature 20-30 °C was considered as optimum for germination...
Tipo: Journal article
Palavras-chave: Acanthoscelides schrankiae; Bruchidae; Germination; Mimosa bimucronata; Seed predation.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400002

Some ultrastructural observations of the vegetative, resting and excysting ciliate, urostyla grandis (urostylidae, hypotrichida)
Provedor de dados: 48
Autores: LIU,ZHAO; YI-SONG,LI; JUN-GANG,LI; FU-KANG,GU.
In order to reveal the differentiation characteristics of organelles of ciliates under different physiological status, the cellular ultrastructure of Urostyla grandis was studied by transmission electron microscopy. In the resting cells most ciliary shafts, kinetosomes and sub-pellicle microtubules were resorbed, and the endoplasmic reticulum (ER) disappeared with the autophagocytosis taking place within the cytoplasm. As well, the nuclear matrix of the macronucleus was extruded into the cytoplasm, forming pseudopodia-like structures with large quantities of heterochromatin (CH) attached to the inner nuclear membrane. During excystment, membraneous structures developed and gradually increased in number to form the ER. Autophagic vacuoles (AVs) appeared...
Tipo: Journal article
Palavras-chave: Urostyla grandis; Ultrastructure; Vegetative cell; Resting cell; Excysting cell.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400001

Role of berberine in ameliorating Schistosoma mansoni-induced hepatic injury in mice
Provedor de dados: 48
Autores: Dkhi,Mohamed A.
BACKGROUND: Schistosomiasis is caused by helminth parasites of the genus Schistosoma. Berberine chloride (BER), an isoquinoline alkaloid, has been used in vivo for its antiparasitic, antioxidant and hepatoprotective properties. In this study, the protective effect of BER and praziquantel has been compared for the extent of schistosomiasis-induced oxidative stress in hepatic tissue of mice. RESULTS: S. mansoni was able to induce inflammation and injury to the liver, evidenced (i) by an increase in inflammatory cellular infiltrations, dilated sinusoids and vacuolated hepatocytes, (ii) by decreased levels of alanine and aspartate aminotransferases and increased levels of alkaline phosphatase, &amp;#947;-glutamyl transferase in the liver homogenate, (iii) by...
Tipo: Journal article
Palavras-chave: Schistosoma mansoni; Berberine; Liver; Mice.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100008

A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies
Provedor de dados: 48
Autores: CAMPOS,ANA MARÍA; SOTOMAYOR,CARLOS PATRICIO; PINO,EDUARDO; LISSI,EDUARDO.
A novel procedure for the evaluation of total reactive antioxidant potentials (TRAP) is described. The method is based on the measurement of the bleaching of pyranine by peroxyl radicals. The addition of the antioxidants produces a clear induction time whose magnitude is directly related to the antioxidant concentration. A comparison of the values obtained with those reported employing closely related methodologies shows that the results are significantly affected by the substrate employed to monitor the steady state free radical concentration. Possible sources of this dependence are discussed
Tipo: Journal article
Palavras-chave: Peroxyl radicals; Polyphenols; Pyranine; Total reactive antioxidant potential.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200015

Francisco Varela (1946-2001):: Filling the mind - brain gap: A life adventure
Provedor de dados: 48
Autores: PALACIOS,ADRIÁN G.; BACIGALUPO,JUAN.
One of the most eminent neuroscientists recently passed away in Paris. Professor Francisco Varela was a scholar that approached science with a remarkably broad and integrative perspective, deeply contributing to a diversity of fields, from mathematics to epistemology, from immunology to neuroscience. He was strongly influenced by Buddhism and actively participated in unraveling the relationship between science and spirituality. This article introduces a special edition of Biological Research dedicated to the memory of this great man. It contains a collection of valuable contributions by various authors who collaborated with Varela at different moments of his outstanding scientific career. Their articles cover most of the fields in which he made...
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100002

Organic and inorganic selenium compounds produce different protein patterns in the blood plasma of rats
Provedor de dados: 48
Autores: MAHN,ANDREA V; TOLEDO,HÉCTOR M; RUZ,MANUEL H.
Some selenium compounds offer important health benefits when administered at supranutritional doses, such as improvement of the immune system and of male fertility, and the prevention of some types of cancer. The traditional selenium indexes do not account for the metabolic status of this element among replete individuals. As a consequence, there is a need for new indexes that distinguish between repletion statuses of selenium. The aim of this work was to indentify some plasmatic proteins that respond to supranutritional doses of selenium, which could be proposed as new protein markers of selenium intake. The effect on rats of dietary supplementation with either selenomethylselenocysteine (SMSeC) or sodium-selenate on some blood plasma proteins was...
Tipo: Journal article
Palavras-chave: Selenium status; Protein abundance; Rat blood plasma; Nutritional biomarkers.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200004

Co-operative effect of glutathione depletion and selenium induced oxidative stress on API and NFkB expression in testicular cells in vitro: insights to regulation of spermatogenesis
Provedor de dados: 48
Autores: SHALINI,SONIA; BANSAL,MOHINDER P.
Previous studies have shown that transcription factors, API and NFkB exert important roles in the process by which selenium regulates spermatogenesis. Glutathione, an intracellular thiol, acts as a source of reducing power and aids in maintenance of the cellular redox status. The activities of selenium are closely related to the availability of glutathione. Presently, mouse testicular cells were cultured in the presence of BSO, a known glutathione depletor, to generate oxidative stress. Selenium (Se) was added as sodium selenite to these cells at concentrations of 0.5 µM and 1.5 µM. It was observed that at 1.5 µM, Se acted as a pro-oxidant and significantly decreased the redox ratio. RT PCR analysis revealed that cjun, cfos expression increased in...
Tipo: Journal article
Palavras-chave: API; BSO; GSH; NFkB; Oxidative stress; Selenium; Testicular cells.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400005

Modulation of nuclear receptor dependent transcription
Provedor de dados: 48
Autores: HART,STEPHEN M.
Nuclear receptors comprise a family of transcription factors that regulate gene expression in a ligand dependent manner. They can activate or repress target genes by binding directly to DNA response elements as homo- or hetero-dimers or by binding to other classes of DNA-bound transcription factors. These activities have been linked to the formation of complexes with molecules that appear to serve as coactivators or corepressors, causing local modification of chromatin structure in order to regulate expression of their target genes. Several members of nuclear receptor family are directly associated with human malignancies including breast cancer, prostate cancer and leukaemia. The pathogenesis of each of these diseases is underpinned by the activities of a...
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200021

A Scientometric view of some Biological disciplines in Chile
Provedor de dados: 48
Autores: KRAUSKOPF,MANUEL.
During the last decade the articles published by Chilean Research Centers grew 1,73 which compares to the 2.34 fold increase of mainstream research articles registered as a whole in Latin America. However, the relative impact of the Chilean publications surpassed that of Latin America. In Biological Sciences, traditionally the strongest research area within Chile, Latin America also shows a steeper slope of growth. Qualitatively, biological disciplines in Chile are comparable to those published in Latin America although in Chile there are specialties as Physiology that surpass the average world's impact. The scientometric data is consistent with the fall in individual grants that the Chilean Research Fund (FONDECYT) has been allocating during the last...
Tipo: Journal article
Palavras-chave: Research output; Biological sciences; Chile; Latin America; Scientometric analyses.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100012

Simultaneous Single Unit Recording in the Mitral Cell Layer of the Rat Olfactory Bulb under Nasal and Tracheal Breathing
Provedor de dados: 48
Autores: AYLWIN,MARÍA DE LA LUZ; DÍAZ,EUGENIA; MALDONADO,PEDRO E.
Odor perception depends on the odorant-evoked changes on Mitral/Tufted cell firing pattern within the olfactory bulb (OB). The OB exhibits a significant "ongoing" or spontaneous activity in the absence of sensory stimulation. We characterized this ongoing activity by simultaneously recording several single neurons in the mitral cell layer (MCL) of anesthetized rats and determined the extent of synchrony and oscillations under nasal and tracheal breathing. We recorded 115 neurons and found no significant differences in the mean firing rates between both breathing conditions. Surprisingly, nearly all single units exhibited a long refractory period averaging 14.4 ms during nasal respiration that was not different under tracheal breathing. We found a small...
Tipo: Journal article
Palavras-chave: Coding; Tetrodes; Spikes; Ongoing activity; Synchrony.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100003

The MAP Kinases are Differently Utilized by CD28 and CD2 Adhesion Pathways in Superantigen-Activated Jurkat T cells
Provedor de dados: 48
Autores: VISSE,EDWARD; INOSTROZA,JUAN; CABELLO,GERTRUDIS; PARRA,EDUARDO.
To mimic the two-signal requirements for T cell activation mediated by ligands, we exposed the superantigens SEA or SEE (signal 1) to T cells incubated with HLA-DR/LFA-3 or HLA-DR/B7-1-CHO transfected cells (signal 2). LFA-3 costimulation was able to induce T cell proliferation as well as IFN-g and IL-4 production at similar levels as in cells induced by B7-1. Analysis of the CD28RE of the IL-2 promoter showed specific transcription factor recruitment at the CD28RE element upon induction by B7-1/SEE. Further functional studies with an IL-2 enhancer-promoter carrying either wild type or mutated versions of the CD28RE site revealed that this element is necessary for full activation upon B7-1 costimulation. While both CD28/B7-1 and CD2/LFA-3 costimulation...
Tipo: Journal article
Palavras-chave: CD28 response element; Staphylococcal Enterotoxin A-E; Extracellular signal regulated kinase; C-Jun N-terminal kinase; Interleukin-2.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200016

VEGFA polymorphisms and cardiovascular anomalies in 22q11 microdeletion syndrome: a case-control and family-based study
Provedor de dados: 48
Autores: CALDERÓN,JUAN FRANCISCO; PUGA,ALONSO R; GUZMÁN,M. LUISA; ASTETE,CARMEN PAZ; ARRIAZA,MARTA; ARACENA,MARIANA; ARAVENA,TERESA; SANZ,PATRICIA; REPETTO,GABRIELA M.
Microdeletion 22q11 in humans causes velocardiofacial and DiGeorge syndromes. Most patients share a common 3Mb deletion, but the clinical manifestations are very heterogeneous. Congenital heart disease is present in 50-80% of patients and is a significant cause of morbidity and mortality. The phenotypic variability suggests the presence of modifiers. Polymorphisms in the VEGFA gene, coding for the vascular endothelial growth factor A, have been associated with non-syndromic congenital heart disease, as well as with the presence of cardiovascular anomalies in patients with microdeletion 22q11. We evaluated the association of VEGFA polymorphisms c.-2578C&gt;A (rs699947), c.-1154G&gt;A (rs1570360) and c.-634C&gt;G (rs2010963) with congenital heart disease in...
Tipo: Journal article
Palavras-chave: Congenital heart disease; DiGeorge syndrome; Genetic modifiers; VEGFA; Velocardiofacial syndrome.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400007

Lipid peroxidation and antioxidant status in blood of patients with uterine myoma, endometrial polypus, hyperplastic and malignant endometrium
Provedor de dados: 48
Autores: PEJIC,SNEZANA; KASAPOVIC,JELENA; TODOROVIC,ANA; STOJILJKOVIC,VESNA; PAJOVIC,SNEZANA B.
Oxidative stress is considered to be involved in pathogenesis of many disorders of the female genital tract. In this study, we explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of uterine diseases in order to evaluate the extent of oxidative stress in blood of such patients. Blood samples of healthy subjects and gynecological patients were collected and subjected to assays for superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and lipid hydroperoxides. The results show that alterations of measured parameters vary with the enzyme type and diagnosis. However, both reduction in antioxidants and elevation of lipid peroxidation were observed in general. Lipid...
Tipo: Journal article
Palavras-chave: Antioxidant enzymes; Endometrial polypus; Endometrial hyperplasia; Endometrial adenocarcinoma; Lipid peroxidation; Uterine myoma.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500005

XVI Reunión Anual de la Sociedad de Biología Celular de Chile
Provedor de dados: 48
Autores: ROSEMBLATT,MARIO.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300005

miR-205 promotes proliferation and invasion of laryngeal squamous cell carcinoma by suppressing CDK2AP1 expression
Provedor de dados: 48
Autores: Zhong,Gang; Xiong,Xingao.
BACKGROUND: The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. miR-205 was reported to be upregulated in laryngeal squamous cell carcinoma (LSCC) tissues, however, the mechanisms by which miR-205 functions as a regulator of LSCC are largely unknown RESULTS: In this study, Real-time qPCR and Western blot assay showed that expression of miR-205 was upregulated and expression of cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was downregulated in LSCC tissues. The expression levels of miR-205 were negatively related to those of CDK2AP1 in LSCC tissues and cell lines. Moreover, we found that miR-205 was the upstream regulator of CDK2AP1 and could suppress the CDK2AP1 expression in LSCC cells....
Tipo: Journal article
Palavras-chave: MiR-205; Laryngeal squamous cell carcinoma (LSCC); CDK2AP1; C-Myc; CyclinD1.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100060

The parietal association cortex of the rat
Provedor de dados: 48
Autores: TORREALBA,FERNANDO; VALDÉS,JOSÉ LUIS.
Spatial cognition is a complex higher function in mammals and is involved in a variety of tasks that can be explored in the laboratory. In this review we will discuss the role of the posterior parietal/anteromedial cortex of rodents, also known as the parietal association cortex, and the hippocampal formation in spatial navigation. We will also discuss other higher associational functions of the posterior parietal/anteromedial cortex as they relate to Dr. Pinto-Hamuy's contribution to understanding behavioral functions.
Tipo: Journal article
Palavras-chave: Association cortex; Spatial navigation; Visual cortex.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400002

Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro
Provedor de dados: 48
Autores: SOTO,CAROLINA R; ARROYO,JORGE; ALCAYAGA,JULIO.
Acetylcholine (ACh), adenosine 5'-triphosphate (ATP) and sodium cyanide (NaCN) activate petrosal ganglion (PG) neurons in vitro, and evoke ventilatory reflexes in situ, which are abolished after bilateral chemosensory denervation. Because in our previous experiments we superfused the isolated PG with solutions free of CO2 /HCO3¯ buffer, we studied its effects on the PG responses evoked in vitro. PGs from adult cats were superfused at a constant pH, with HEPES-supplemented (5 mM) saline with or without CO2 /HCO3¯ (5% / 26.2 mM) buffer, and carotid (sinus) nerve frequency discharge (ƒCN) recorded. Increases in ƒCN evoked by ACh, ATP and NaCN in CO2-free saline were significantly reduced (P&lt;0.05, Wilcoxon test) when CO2 / HCO3¯ was present in the...
Tipo: Journal article
Palavras-chave: Petrosal Ganglion; Carotid sinus nerve; Carbon dioxide; Bicarbonate; Acetylcholine; Adenosine 5'- triphosphate; Cyanide.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300010

Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México
Provedor de dados: 48
Autores: Villa-Ruano,Nemesio; Zurita-Vásquez,Guilibaldo G; Pacheco-Hernández,Yesenia; Betancourt-Jiménez,Martha G; Cruz-Durán,Ramiro; Duque-Bautista,Horacio.
We report the results of in vitro anti-lipase and antioxidant assays using crude ethanolic extracts from 30 plants grown in Oaxaca, México. Anti-lipase tests were performed by using porcine pancreatic lipase (PPL) [EC 3.1.1.3] from Affymetrix/USB. The extracts of Solanum erianthum, Salvia microphylla, Brungmansia suaveolens and Cuphea aequipetala showed up to 60% PPL inhibition. The effect of these extracts on the kinetic parameters of PPL (Km= 0.36 mM, and Vmax=0.085 mM min -1) revealed that the alcoholic preparations of S. erianthum and C. aequipetala engendered a non-competitive inhibition (Vmax=0.055 mM min -1; Vmax= 0.053 mM min -1), whereas those of S. microphylla and B. suaveolens produced a mixed inhibition (Km= 0.567 mM, Vmax=0.051 mM min _1;...
Tipo: Journal article
Palavras-chave: Phytotherapy; Anti-obesity; Antioxidant; Oaxacan ethnobotany.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200006

Malignant melanoma in Chile: different site distribution between private and state patients
Provedor de dados: 48
Autores: Zemelman,Viviana B; Valenzuela,Carlos Y; Sazunic,Ivo; Araya,Irene.
BACKGROUND: The body site location of primary Malignant Melanoma (MM) has been correlated with prognosis and survival. Ethnic, genetics, sun exposure factors are related to the anatomical distribution of MM. Low and high socioeconomic strata in Chile differ in ethnic, genetic and cultural conditions. The purpose of this study was to analyze the anatomical MM distribution in the Chilean population in both strata searching for differences due to their ethno-genetic-cultural differences. Records of 1148 MM, 575cases from state hospitals (Low Socioeconomic Strata, LSS) and 573 cases from private clinics (High Socioeconomic Strata, HSS) were analyzed by body site. RESULTS: Females from LSS showed a higher number of MM in soles, cheeks, and around the eye area....
Tipo: Journal article
Palavras-chave: Chile; Malignant melanoma; Site distribution; Socioeconomic strata.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100034

Cloning and comparison of ten gene sequences of a Chilean H. pylori strain with other H. pylori strains revealed higher variability for VacA and CagA virulence factors
Provedor de dados: 48
Autores: MÜLLER,ILSE; MEDINA-SELBY,ANGELICA; PALACIOS,JOSE LUIS; MARTINEZ,PATRICIO; OPAZO,PATRICIO; BRUCE,ELSA; MANCILLA,MARTA; VALENZUELA,PABLO; YUDELEVICH,ARTURO; VENEGAS,ALEJANDRO.
We have cloned and sequenced ten Helicobacter pylori genes from a Chilean strain (CH-CTX1) including: a cytotoxin VacA fragment, a CagA fragment (A17), a species-specific protein (TsaA), urease subunits (UreA, UreB), a flagellin subunit (FlaB), heat shock proteins (HspA and HspB), adhesin (HpaA) and a lipoprotein (Lpp20). We compared their deduced amino acid sequences with the corresponding sequences from three unrelated H. pylori strains, including fully sequenced strains 26695(UK) and J99(USA), and found that eight of them (UreA, UreB, FlaB, HspA, HspB, Lpp20, TsaA and HpaA) presented more than 97.3% identity. In contrast, VacA partial sequence showed lower identity values (93.2 - 94.9%). Moreover, we found major differences in the A17 region respect to...
Tipo: Journal article
Palavras-chave: Chilean H. pylori strain; PCR-cloned genes; HpaA; VacA; CagA sequences.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100010

Plasmid-Encoded Multidrug Resistance: A Case study of Salmonella and Shigella from enteric diarrhea sources among humans
Provedor de dados: 48
Autores: Suh Yah,Clarence.
Salmonellosis and shigellosis are signifcant and persistent causes of diarrheal diseases among humans in developing countries. With that in mind, the current study investigates the occurrence of plasmid-encoded multidrug resistances in Salmonella and Shigella from diarrheal cases among humans. The isolates were characterized by serotyping, antimicrobial-susceptibility testing, transfer experiments and curing. The extended spectrum &amp;#946;-lactamase (ESBL) was detected by the double disc diffusion synergy test (DDST). A signifcant number of the plasmid-encoded multidrug resistant (PEMDR) Salmonella and Shigella isolates were found to harbour transferable plasmid genes resistant to antibiotics like ampicillin, chloramphenicol,...
Tipo: Journal article
Palavras-chave: Salmonella-Shigella; Plasmid-encoded; Multidrug-resistant.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200001

Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
Provedor de dados: 48
Autores: Bae,Seunghee; Jung,Younjung; Choi,Yeong Min; Li,Shunhua.
BACKGROUND: Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown. RESULTS: This study investigated the effects of EMS on TNF-&amp;#945;-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-&amp;#945;-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IkB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-kB....
Tipo: Journal article
Palavras-chave: Er-Miao-San MMP1 Human dermal fibroblasts TNF-&amp;#945; NF-kB.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100009

Molecular and pathological basis of aceruloplasminemia
Provedor de dados: 48
Autores: KONO,SATOSHI; MIYAJIMA,HIROAKI.
Aceruloplasminemia is an autosomal recessive neurodegenerative disease characterized by iron accumulation in the brain as well as visceral organs. It is a loss-of-function disorder caused by mutations in the ceruloplasmin gene. Clinically, this disease consists of the triad of adult-onset neurological disease, retinal degeneration and diabetes mellitus. Massive iron accumulation and extensive loss of neurons are observed in the basal ganglia. The elevated iron concentration is associated with increased lipid peroxidation in the brains of aceruloplasminemia patients. Enlarged or deformed astrocytes and spheroid-like globular structures are characteristic neuropathological findings in aceruloplasminemia. Moreover, deformed astrocytes and globular structures...
Tipo: Journal article
Palavras-chave: Ceruloplasmin; Iron; Lipid peroxidation; Mutation.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100003

Stimulus specificity in the acquisition and extinction of conditioned taste aversion
Provedor de dados: 48
Autores: VOGEL,EDGAR H; SOTO,FABIÁN A; CASTRO,MARÍA E; SOLAR,PAOLA A.
An experiment evaluated whether the acquisition and extinction of conditioned taste aversion in the rat is stimulus-specific by testing the degree of response transfer between sweet and salty tastes. Animals in the paired-same and paired-different groups received a presentation of a gustatory CS and a cyclophosphamide injection US. Nonconditioned control groups received unpaired CS /US presentations or the CS followed by a vehicle injection. Taste avoidance was evaluated in three nonreinforced test sessions. In the paired-same, unpaired and vehicle groups, all test sessions were conducted with the same flavor as originally used in training, whereas the paired-different group was tested with a novel flavor on the first and second sessions and with the...
Tipo: Journal article
Palavras-chave: Conditioning; Cyclophosphamide; Extinction; Generalization; Stimulus specificity; Taste aversion.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200003

Con aportes Milenium: Ponen en marcha el MIFAB, Instituto Milenio  de Biología Fundamental y Aplicada
Provedor de dados: 48
Autores: PRENAFETA J,SERGIO.
Tipo: Journal article
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400002

A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
Provedor de dados: 48
Autores: RIOS,MARIANA; OJEDA,SUANY; VELASQUEZ,LUIS A; MAISEY,KEVIN; CROXATTO,HORACIO B.
Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct. Rats on day 2 of pregnancy were untreated or treated with 10 µg of E2 and the oviducts were extracted at 30, 180 and 360 min later and used to isolate RNA. Products of reverse transcriptase-PCR, made with pairs of arbitrary and oligo-deoxythymidine primers, were separated on denaturing polyacrylamide gels and candidate bands were excised and reamplified. Truly positive cDNA fragments determined by a single strand conformation polymorphism assay were cloned and...
Tipo: Journal article
Palavras-chave: Differential display; Estradiol; Gene; MRNA; Oviduct.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011

Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer
Provedor de dados: 48
Autores: Han,Xu; Wang,Lingling; Ning,Yu; Li,Shuang; Wang,Zhenjun.
BACKGROUND AND OBJECTIVE: Long non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC. METHODS: Relative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot. RESULTS: Relative expression of AFAP1-AS1 was significantly elevated in...
Tipo: Journal article
Palavras-chave: AFAP1-AS1; Colorectal cancer; Tumor metastasis; Epithelial-mesenchymal transition (EMT).
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100036

The possible role of Myosin light chain in myoblast proliferation
Provedor de dados: 48
Autores: ZHANG,SU-ZHEN; XU,YONG; XIE,HUI-QI; LI,XIU-QUN; WEI,YU-QUAN; YANG,ZHI-MING.
Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this report, we studied the possible role of Myl in the proliferation of skeletal muscle-derived myoblasts. Compared to diaphragm-derived myoblasts, the extraocular muscle-derived myoblasts with lower levels of Myl proliferated faster, maintained a longer proliferation phase, and formed more final myotubes. It was found that blockading Myl with anti-Myl antibody or knockdown of Myll by siRNA targeted against Myll could enhance the myoblast...
Tipo: Journal article
Palavras-chave: Myosin light chain; Proliferation; Myoblast; Extraocular muscle.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100013

Prenatal testosterone treatment alters LH and testosterone responsiveness to GnRH agonist in male sheep
Provedor de dados: 48
Autores: RECABARREN,SERGIO E; LOBOS,ALEJANDRO; FIGUEROA,YARA; PADMANABHAN,VASANTHA; FOSTER,DOUGLAS L; SIR-PETERMANN,TERESA.
Although evidence is accumulating that prenatal testosterone (T) compromises reproductive function in the female, the effects of excess T in utero on the postnatal development of male reproductive function has not been studied. The aim of this study was to assess the influence of prenatal T excess on age-related changes in pituitary and gonadal responsiveness to GnRH in the male sheep. We used the GnRH agonist, leuprolide (10 µg/kg), as a pharmacologic challenge at 5, 10, 20 and 30 weeks of age. These time points correspond to early and late juvenile periods and the prepubertal and postpubertal periods of sexual development, respectively. LH and T were measured in blood samples collected before and after GnRH agonist administration. The area under the...
Tipo: Journal article
Palavras-chave: Fetal programming; IUGR; Pituitary sensitivity.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400007

A simple and effective pressure culture system modified from a transwell cell culture system
Provedor de dados: 48
Autores: Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian.
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin &amp;#946;1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is...
Tipo: Journal article
Palavras-chave: Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007

Are levels of digestive enzyme activity related to the natural diet in passerine birds?
Provedor de dados: 48
Autores: Ramírez-Otárola,Natalia; Sabat,Pablo.
Digestive capabilities, such as the rates nutrient hydrolysis and absorption, may affect energy intake and ultimately feeding behavior. In birds, a high diversity in gut biochemical capabilities seems to support the existence of a correlation between the morphology and physiology of the intestinal tract and chemical features of the natural diet. However, studies correlating the activity of digestive enzymes and the feeding habits at an evolutionary scale are scarce. We investigated the effect of dietary habits on the digestive physiological characteristics of eight species of passerine birds from Central Chile. The Order Passeriformes is a speciose group with a broad dietary spectrum that includes omnivorous, granivorous and insectivorous species. We...
Tipo: Journal article
Palavras-chave: Birds; Diet; Intestinal enzymes; Passeriformes; Food habits; Adaptation.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100011

A radiographic method to estimate lung volume and its use in small mammals
Provedor de dados: 48
Autores: CANALS,MAURICIO; OLIVARES,RICARDO; ROSENMANN,Mario.
In this paper we develop a method to estimate lung volume using chest x-rays of small mammals. We applied this method to assess the lung volume of several rodents. We showed that a good estimator of the lung volume is: V*L = 0.496 · V RX &amp;#8776; 1/2·V RX , where V RX is a measurement obtained from the x-ray that represents the volume of a rectangular box containing the lungs and mediastinum organs. The proposed formula may be interpreted as the volume of an ellipsoid formed by both lungs joined at their bases. When that relationship was used to estimate lung volume, values similar to those expected from allometric relationship were found in four rodents. In two others, M. musculus and R. norvegicus, lung volume was similar to reported data, although...
Tipo: Journal article
Palavras-chave: Lung volume; X-ray; Method; Rodents.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100006

Moderation in Australia-Policy and Achievements
Provedor de dados: 48
Autores: STOCKLEY,CREINA.
Alcohol has been consumed in Australia since European settlement in 1788. In 1998, approximately 60 % of Australians consumed an alcoholic beverage at least once per week. The effects of alcohol on the human body are dose dependent, where the harmful effects of alcohol are generally observed only when alcohol consumption exceeds moderate consumption levels of 30 to 40 g of alcohol per day. The discovery that a J-shaped curve described the relationship between level of alcohol consumption and risk of cardiovascular disease was, however, only made in 1990_cardiovascular disease is the leading cause of death in the western world. Thus prior to 1990, Australian public health policy focused primarily on the harmful effects of alcohol consumption and the health...
Tipo: Journal article
Palavras-chave: Alcohol; Guidelines moderation moderation policy recommendation wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200005

Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
Provedor de dados: 48
Autores: Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R.
Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine...
Tipo: Journal article
Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005

Awareness and current knowledge of breast cancer
Provedor de dados: 48
Autores: Akram,Muhammad; Iqbal,Mehwish; Daniyal,Muhammad; Khan,Asmat Ullah.
Abstract Breast cancer remains a worldwide public health dilemma and is currently the most common tumour in the globe. Awareness of breast cancer, public attentiveness, and advancement in breast imaging has made a positive impact on recognition and screening of breast cancer. Breast cancer is life-threatening disease in females and the leading cause of mortality among women population. For the previous two decades, studies related to the breast cancer has guided to astonishing advancement in our understanding of the breast cancer, resulting in further proficient treatments. Amongst all the malignant diseases, breast cancer is considered as one of the leading cause of death in post menopausal women accounting for 23% of all cancer deaths. It is a global...
Tipo: Journal article
Palavras-chave: Breast cancer; Chemotherapy; Gene therapy; Stem cell therapy.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100504

Extracellular signals, cell interactions and transcription factors involved in the induction of the neural crest cells
Provedor de dados: 48
Autores: AYBAR,MANUEL J.; GLAVIC,ALVARO; MAYOR,ROBERTO.
The neural crest is induced at the border between the neural plate and the epidermis. A complex set of signals is required for the specification of the crest cells between the epidermis and the neural plate. Here we discuss evidence supporting a model for neural crest induction in which different signals contribute in a sequential order. First, a gradient of bone morphogenic proteins (BMPs) is established in the ectoderm that results in segreggation into neural plate, neural folds and epidermis at increasing levels of BMP activity. Thus, the neural folds are induced at a precise threshold concentration of BMP, but this neural fold has an anterior character. In a second step, these anterior neural folds are transformed into prospective neural crest by...
Tipo: Journal article
Palavras-chave: Neural crest induction BMPs Wnts FGF retinoic acid Notch; Delta.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200018

Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells
Provedor de dados: 48
Autores: Mikami,Taro; Yoshida,Keiichiro; Sawada,Hajime; Esaki,Michiyo; Yasumura,Kazunori; Ono,Michio.
BACKGROUND: The collective cell migration of stratified epithelial cells is considered to be an important phenomenon in wound healing, development, and cancer invasion; however, little is known about the mechanisms involved. Furthermore, whereas Rho family proteins, including RhoA, play important roles in cell migration, the exact role of Rho-associated coiled coil-containing protein kinases (ROCKs) in cell migration is controversial and might be cell-type dependent. Here, we report the development of a novel modified scratch assay that was used to observe the collective cell migration of stratified TE-10 cells derived from a human esophageal cancer specimen. RESULTS: Desmosomes were found between the TE-10 cells and microvilli of the surface of the cell...
Tipo: Journal article
Palavras-chave: Collective cell migration; Stratified epithelium; TE-10 cells; Rho-associated coiled coil-containing protein kinase; SiRNA.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100048

Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.
Provedor de dados: 48
Autores: MAYA,JUAN DIEGO; RODRÍGUEZ,ANDRÉS; PINO,LAURA; PABÓN,ADRIANA; FERREIRA,JORGE; PAVANI,MARIO; REPETTO,YOLANDA; MORELLO,ANTONIO.
Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growth phase. Nifurtimox and benznidazole, used in the treatment of Chagas' disease, decreased metallothionein activity by approximately 70%. T. cruzi metallothionein was induced by ZnCl2. Metallothionein from T. cruzi was partially purified and its monobromobimane derivative showed a molecular weight of approximately 10,000 Da by SDS-PAGE analysis. The concentration of trypanothione, the major glutathione conjugate in T. cruzi, ranged from 3.8 to 10.8 nmol/mg protein,...
Tipo: Journal article
Palavras-chave: Trypanosoma cruzi; Metallothionein; Glutathione; Trypanothione; Benznidazole; Nifurtimox; Buthionine sulfoximine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007

IMPORTANTE PARTICIPACION DE CHILE EN EL CONGRESO FEBS/PABMB 2001
Provedor de dados: 48
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200005

High gender -specific susceptibility to curare- a neuromuscular blocking agent
Provedor de dados: 48
Autores: Maurya,Santosh K; Periasamy,Muthu; Bal,Naresh C.
Curare, a selective skeletal muscle relaxant, has been used clinically to reduce shivering and as an anesthetic auxiliary in abdominal surgery. It is also widely used in animal experiments to block neuromuscular junction activity. Effective doses of curare diminish muscle contraction without affecting brain function, but at higher doses it is known to be lethal. However, the exact dose of curare initiating muscle relaxation vs. lethal effect has not been fully characterized in mice. In this study we carefully examined the dose-response for achieving muscle inactivity over lethality in both male and female mice (C57BL6/J). The most striking finding of this study is that female mice were highly susceptible to curare; both the EDm and LDm were at least 3-fold...
Tipo: Journal article
Palavras-chave: Curare; Dose response; Gender; Muscle activity; Oxygen consumption.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100011

Hemoglobin affinity in Andean rodents
Provedor de dados: 48
Autores: OSTOJIC,HRVOJ; CIFUENTES,VERONICA; MONGE,CARLOS.
Blood hemoglobin oxygen affinity (P50) was measured in three Andean species and in the laboratory rat (control), all raised near sea level. Chinchilla lanigera (Molina, 1792) has an altitudinal habitat range from low Andean slopes up to 3000 m., while Chinchilla brevicaudata (Waterhouse, 1848) has an altitudinal range from 3000 to 5000 m. The laboratory type guinea pig, wild type guinea pig (Cavia porcellus), (Waterhouse, 1748), and laboratory rat (Rattus norvegicus) were also raised at sea level. The Andean species had high hemoglobin oxygen affinities (low P50) compared with the rat. Chinchilla brevicaudata had a higher affinity than Chinchilla lanigera. The wild type guinea pig had a higher affinity than the laboratory type. As has been shown in other...
Tipo: Journal article
Palavras-chave: Hemoglobin affinity; Rodents; High altitude adaptation.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100005

Trait anxiety affects the orofacial nociceptive response in rats
Provedor de dados: 48
Autores: Matos,Anne Caroline C; Teixeira-Silva,Flavia; Goes,Tiago C; Quintans Júnior,Lucindo J; Albuquerque Júnior,Ricardo Luiz C; Bonjardim,Leonardo R.
The aims of the present study were to assess the influence of: a) trait anxiety on orofacial pain; and b) orofacial pain on state anxiety. Forty-four rats were initially exposed to the free-exploratory paradigm for the evaluation of their anxiety profiles. In accordance to the parameter "Percentage of time in the novel side", the animals were considered as presenting high or low levels of trait anxiety when presenting values below the 1st quartile, or above the 3rd quartile, respectively. A week later, formalin-1.5% was injected into the upper lip of each animal. The behavioural nociceptive response, characterized by increased orofacial rubbing (OR), was quantified for 30 minutes, as follows: Total time OR (0-30 minutes: total pain), 1st phase OR (0-6...
Tipo: Journal article
Palavras-chave: Trait anxiety; Formalin test; Orofacial nociception; State anxiety.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400007

A Tribute to Professor Gustavo Hoecker Salas
Provedor de dados: 48
Autores: Ferreira,Arturo; Valenzuela,Carlos; Santos,Manuel J.
Tipo: Journal article
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300001

A comparison of methods employed to evaluate antioxidant capabilities
Provedor de dados: 48
Autores: PEREZ,DRUSO D; LEIGHTON,FEDERICO; ASPEE,ALEXIS; ALIAGA,CAROLINA; LISSI,EDUARDO.
Thrce ditferent methodologies frequently employed to evaluate the indexes that report the antioxidant capabilities of pure compounds and/or complex mixtures of antioxidants are applied to a series of mono- and polyphenols, as well as to two wine (red and white) samples. These methodologies are based on the bleaching of a stable radical, the effect of the additive upon luminol chemiluminescence induced by peroxyl radicals, and the effect of the additive upon the bleaching of the fluorescence from a dye molecule. Widely ditferent responses are obtained from the different methodologies. These differences are interpreted in terms of the different factors (stoichiametric factors and/or reactivities) that determines the indexes evaluated by these different...
Tipo: Journal article
Palavras-chave: ABTS radical bleaching; Antioxidants; C-phycocyanin bleaching; Total antioxidant reactivity; Total reactive antioxidant potential.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200006

Gastrodia elata Blume and its pure compounds protect BV-2 microglial-derived cell lines against &amp;#946;-amyloid: The involvement of GRP78 and CHOP
Provedor de dados: 48
Autores: Geum-Hwa,Lee; Hyung-Ryong,Kim; Sang-Yong,Han; Bidur,Bhandary; Do-Sung,Kim; Min-Gul,Kim; Byung-Ok,So; Sun-Young,Kim; Kyu-Sik,Jo; Bo-Hee,Lee; Hee-Nam,Seo; Soo-Wan,Chae; Han-Jung,Chae.
Objectives: Gastrodia elata (GE) Blume (Orchidaceae) has been previously known for its therapeutic benefits against neurodegenerative diseases. Microglial activation and death have been implicated in the pathogenesis of a variety of neurodegenerative diseases, including Alzheimer's disease. In this study, GE and its pure components, gastrodin and 4-hydroxybenzyl alcohol (4HBA), were applied to &amp;#946;-amyloid-induced BV2 mouse microglial cells. Materials and Methods Cell viability was assessed by the MTT assay and Western blotting was also performed. Results: &amp;#946;-amyloid-induced cell death was shown to be induced time- and dose-dependently. To examine the cell death mechanism, we confirmed the involvement of ER stress signaling. C/EBP homologous...
Tipo: Journal article
Palavras-chave: Gastrodia elata 4HBA GRP78 CHOP &amp;#946; -amyloid.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400013

The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
Provedor de dados: 48
Autores: URBINA,DANIELA C; SILVA,HERMAN; MEISEL,LEE A.
Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism
Tipo: Journal article
Palavras-chave: Arabidopsis thaliana; Ca2+-ATPases; Calcium; Gravitropism; Thapsigargin; Root.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200011

Disentangling the dynamic core: a research program for a neurodynamics at the large-scale
Provedor de dados: 48
Autores: LE VAN QUYEN,MICHEL.
My purpose in this paper is to sketch a research direction based on Francisco Varela's pioneering work in neurodynamics (see also Rudrauf et al. 2003, in this issue). Very early on he argued that the internal coherence of every mental-cognitive state lies in the global self-organization of the brain activities at the large-scale, constituting a fundamental pole of integration called here a "dynamic core". Recent neuroimaging evidence appears to broadly support this hypothesis and suggests that a global brain dynamics emerges at the large scale level from the cooperative interactions among widely distributed neuronal populations. Despite a growing body of evidence supporting this view, our understanding of these large-scale brain processes remains hampered...
Tipo: Journal article
Palavras-chave: Nonlinear dynamics; Phase synchronization; Chaos; EEG; Non-gaussian statistics.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100006

Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
Provedor de dados: 48
Autores: Cheng,Jin; Yi,Zhang; Jiping,Li.
BACKGROUND: Accumulating evidence has confirmed that miR-196a plays a critical role in tumorigenesis and tumor progression in a variety of cancers. It has been demonstrated that miR-196a is highly up-regulated in laryngeal cancer by miRNA profiling analysis. However, the functional mechanism of miR-196a in laryngeal cancer remains unclear. This study aims to explore the mechanism of miR-196a in laryngeal cancer METHODS: In the present study, we conducted qPCR analysis of miR-196a expression in human laryngeal cancer and showed that miR-196a was overexpressed in tumor-derived samples and laryngeal cancer cell lines compared with matched normal controls. Further functional analysis of miR-196a demonstrated that the inhibition of miR-196a could inhibit...
Tipo: Journal article
Palavras-chave: MiR-196a; P27kp1; Laryngeal cancer.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100040

Hormesis response of marine and freshwater luminescent bacteria to metal exposure
Provedor de dados: 48
Autores: SHEN,KAILI; SHEN,CHAOFENG; LU,YUAN; TANG,XIANJIN; ZHANG,CONGKAI; CHEN,XINCAI; SHI,JIYAN; LIN,QI; CHEN,YINGXU.
The stimulatory effect of low concentrations of toxic chemicals on organismal metabolism, referred to as hormesis, has been found to be common in the widely used luminescence bioassay. This paper aims to study the hormesis phenomenon in both marine and freshwater luminescent bacteria, named Photobacterium phosphorem and Vibrio qinghaiensis. The effects of Cu (II), Zn (II), Cd (II) and Cr (VI) on luminescence of these two bacteria were studied for 0 to 75 minutes exposure by establishing dose- and time-response curves. A clear hormesis phenomenon was observed in all four testing metals at low concentrations under the condition of luminescence assays.
Tipo: Journal article
Palavras-chave: Hormesis; Bioluminescence; Q67; T3.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200006

TERCER SIMPOSIO INTERNACIONAL SOBRE PROTEINA QUINASA CK 2 SE REALIZÓ EN CHILE
Provedor de dados: 48
Autores: ALLENDE,JORGE E.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100006

stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
Provedor de dados: 48
Autores: Berrocal,Liliana; Fuentes,Juan A; Nicole Trombert,A; Jofré,Matías R; Villagra,Nicolás A; Valenzuela,Luis M; Mora,Guido C.
BACKGROUND: Salmonella enterica serovar Typhi (S. Typhi) stg operon, encoding a chaperone/usher fimbria (CU), contributes to an increased adherence to human epithelial cells. However, one report suggests that the presence of the Stg fimbria impairs the monocyte-bacteria association, as deduced by the lower level of invasion to macrophage-like cells observed when the stg fimbrial cluster was overexpressed. Nevertheless, since other CU fimbrial structures increase the entry of S. Typhi into macrophages, and considering that transcriptomic analyses revealed that stg operon is indeed expressed in macrophages, we reassessed the role of the stg operon in the interaction between S. Typhi strain STH2370 and human cells, including macrophage-like cells and...
Tipo: Journal article
Palavras-chave: Salmonella Typhi; Stg; Fimbriae; Adherence; Association bacteria-eukaryotic cells.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100034

Phytochemical and antioxidant studies of Laurera benguelensis growing in Thailand
Provedor de dados: 48
Autores: Manojlovic,Nedeljko T; Vasiljevic,Perica J; Gritsanapan,Wandee; Supabphol,Roongtawan; Manojlovic,Ivana.
The aim of this study was to investigate metabolites of the lichen Laurera benguelensis. A high-performance liquid chromatographic (HPLC) method has been developed for the characterization of xanthones and anthraquinones in extracts of this lichen. Lichexanthone, secalonic acid D, norlichexanthon, parietin, emodin, teloschistin and citreorosein were detected in the lichen samples, which were collected from two places in Thailand. Components of the lichen were identifed by relative retention time and spectral data. This is the frst time that a detailed phytochemical analysis of the lichen L. benguelensis was reported and this paper has chemotaxonomic signifcance because very little has been published on the secondary metabolites present in Laurera species....
Tipo: Journal article
Palavras-chave: Anthraquinones; Antioxidant activity; Laurera benguelensis; Xanthones.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200004

The anteromedial extrastriate complex is critical for the use of allocentric visual cues and in the retention of the Lashley III maze task in rats
Provedor de dados: 48
Autores: ESPINOZA-CIFUENTES,SERGIO; LEANDER ZEISE,MARC.
The anteromedial extrastriate complex has been proposed to play an essential role in a spatial orientation system in rats. To gain more information about that possible role, in the present work, two questions were addressed: 1. Are allocentric visual cues relevant for acquisition of the orientation task in the Lashley III maze? 2. Is this integration of allocentric inputs in the anteromedial visual complex relevant in the retention of this test? While a control group of rats was trained keeping the maze in the same position, the experimental group was trained with the maze rotated counterclockwise by 144 degrees from session to session. Control rats reached learning criterion significantly earlier and with less errors than the experimental ones (p&lt;.05)....
Tipo: Journal article
Palavras-chave: Visuospatial memory; Anteromedial extrastriate complex; Lashley III maze; Ibotenic acid; Rat.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400006

Quantification of Japanese Quail Eggshell Colour by Image Analysis
Provedor de dados: 48
Autores: SEZER,METIN; TEKELIOGLU,OGUZ.
The Japanese quail lays eggs with colourful and patterned shells which make the eggshell colour difficult to classify. In this study, the method of measuring colour of patchy eggs using image analyses and its power to discriminate among individual variation were established. Estimated repeatability for egg colour and proportion of patterned areas was high (&gt;0.58), suggesting intermedíate or high heritability of eggshell colour characteristics. Three components have been identified as significant in discriminant function analysis. These three components explained 91.4% of the total variance in egg colour characteristics. In cluster analysis, 78.3% of the eggs that were collected from 15 females were correctly classified. This study indicates that...
Tipo: Journal article
Palavras-chave: Eggshell colour; Image analysis; Individual variation; Repeatability.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100010

Fatty acid composition indicating diverse habitat use in coral reef fishes in the Malaysian South China Sea
Provedor de dados: 48
Autores: Arai,Takaomi; Amalina,Razikin; Bachok,Zainudin.
BACKGROUND: In order to understand feeding ecology and habitat use of coral reef fish, fatty acid composition was examined in five coral reef fishes, Thalassoma lunare, Lutjanus lutjanus, Abudefduf bengalensis, Scarus rivulatus and Scolopsis affinis collected in the Bidong Island of Malaysian South China Sea. RESULTS: Proportions of saturated fatty acids (SAFA) ranged 57.2% 74.2%, with the highest proportions in fatty acids, the second highest was monounsaturated fatty acids (MUFA) ranged from 21.4% to 39.0% and the proportion of polyunsaturated fatty acids (PUFA) was the lowest ranged from 2.8% to 14.1%. Each fatty acid composition differed among fishes, suggesting diverse feeding ecology, habitat use and migration during the fishes' life history in the...
Tipo: Journal article
Palavras-chave: Coral fish; Habitat ecology; Migration; South China Sea; Biochemical signature; Malaysia.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100013

Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases
Provedor de dados: 48
Autores: ORTEGA,XIMENA; POLANCO,RUBÉN; CASTAÑEDA,PATRICIA; PEREZ,LUZ M.
The development of an effective hypersensitive response (HR) in any plant system relies, not only in their gene composition and expression, but also on an effective and rapid signal transduction system. Lemon seedlings induce the phenylpropanoid pathway, which results in the de novo biosynthesis of the phytoalexin scoparone, as part of the hypersensitive response against Alternaria alternata. In order to elucidate some of the signaling elements that participate in the development of HR in lemon seedlings, we used several compounds that are known as activators or inhibitors of signal transduction elements in plants or in animal cells. Lemon seedlings treated either with cholera toxin or with phorbol 12-myristate 13-acetate (PMA), in the absence of A....
Tipo: Journal article
Palavras-chave: Citrus limon; Hypersensitive response; Signal transduction.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300012

Celecoxib decreases growth and angiogenesis and promotes apoptosis in a tumor cell line resistant to chemotherapy
Provedor de dados: 48
Autores: Rosas,Carlos; Sinning,Mariana; Ferreira,Arturo; Fuenzalida,Marcela; Lemus,David.
BACKGROUND: During the last few years it has been shown in several laboratories that Celecoxib (Cx), a non-steroidal anti-inflammatory agent (NSAID) normally used for pain and arthritis, mediates antitumor and antiangiogenic effects. However, the effects of this drug on a tumor cell line resistant to chemotherapeutical drugs used in cancer have not been described. Herein we evaluate the angiogenic and antitumor effects of Cx in the development of a drug-resistant mammary adenocarcinoma tumor (TA3-MTXR). RESULTS: Cx reduces angiogenesis in the chick embryonic chorioallantoic membrane assay (CAM), inhibits the growth and microvascular density of the murine TA3-MTXR tumor, reduces microvascular density of tumor metastases, promotes apoptosis and reduces...
Tipo: Journal article
Palavras-chave: Angiogenesis; Celecoxib; Tumor.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100027

Neuron-derived CCL2 contributes to microglia activation and neurological decline in hepatic encephalopathy
Provedor de dados: 48
Autores: Zhang,Li; Tan,Jinyun; Jiang,Xiaoping; Qian,Weiwei; Yang,Ting; Sun,Xijun; Chen,Zhaohui; Zhu,Qiwen.
Abstract  Background  CCL2 was up-regulated in neurons and involved in microglia activation and neurological decline in mice suffering from hepatic encephalopathy (HE). However, no data exist concerning the effect of neuron-derived CCL2 on microglia activation in vitro.  Methods  The rats were pretreated with CCL2 receptor inhibitors (INCB or C021, 1 mg/kg/day i.p.) for 3 days prior to thioacetamide (TAA) administration (300 mg/kg/day i.p.) for inducing HE model. At 8 h following the last injection (and every 4 h after), the grade of encephalopathy was assessed. Blood and whole brains were collected at coma for measuring CCL2 and Iba1 expression. In vitro, primary neurons were stimulated with TNF-&amp;#945;, and then the medium were collected for addition...
Tipo: Journal article
Palavras-chave: Hepatic encephalopathy; Neuron; Microglia; Chemokine CC motif ligand 2.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100218

Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
Provedor de dados: 48
Autores: MARRERO,PATRICIA; FREGEL,ROSA; CABRERA,VICENTE M; NOGALES,MANUEL.
DNA extraction methods for genotyping non-invasive samples have led to great advances in molecular research for ecological studies, and have been particularly useful for analyzing threatened species. However, scarce amounts of fragmented DNA and the presence of Taq polymerase inhibitors in non-invasive samples are potential problems for subsequent PCR amplifications. In this study we describe a novel technique for extracting DNA from alimentary tract cells found on external surfaces of feces and regurgitated seeds. The presence of contaminants and inhibitors is minimized and samples are preserved intact for use in other ecological research (e.g. trophic studies). The amplification efficiency and purity of the extracted DNA from feces were significantly...
Tipo: Journal article
Palavras-chave: DNA extraction; Ecological studies; Guanidine thiocyanate; Non-invasive method; Pigeons; Seed dispersal.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200002

SH Oxidation Stimulates Calcium Release Channels (Ryanodine Receptors) From Excitable Cells
Provedor de dados: 48
Autores: HIDALGO,CECILIA; BULL,RICARDO; MARENGO,JUAN J; PÉREZ,CLAUDIO F; DONOSO,PAULINA.
The effects of redox reagents on the activity of the intracellular calcium release channels (ryanodine receptors) of skeletal and cardiac muscle, or brain cortex neurons, was examined. In lipid bilayer experiments, oxidizing agents (2,2'-dithiodipyridine or thimerosal) modified the calcium dependence of all single channels studied. After controlled oxidation channels became active at sub &amp;micro;M calcium concentrations and were not inhibited by increasing the calcium concentration to 0.5 mM. Subsequent reduction reversed these effects. Channels purified from amphibian skeletal muscle exhibited the same behavior, indicating that the SH groups responsible for modifying the calcium dependence belong to the channel protein. Parallel experiments that...
Tipo: Journal article
Palavras-chave: Calcium dependence; Neurons; Redox state; Ryanodine receptors; Sarcoplasmic reticulum; Skeletal and cardiac muscle.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200011

Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
Provedor de dados: 48
Autores: GHATPANDE,SATISH; SHAFIQ,SAIYID; SIDDIQUI,M.A.Q.
Recent gene knock-out studies in mice have suggested that ventricular myosin light chain-2 (vMLC2) has a role in the regulation of cardiogenic development and that perturbation in expression of vMLC2 is linked to the onset of dilated cardiomyopathy. In an attempt to develop an avian model for such studies, we examined the expression pattern of vMLC2 in chicken embryos at various stages and analyzed the effect of antisense oligonucleotide-mediated interference of vMLC2 function in cultures of whole embryos. Our results showed vMLC2 to be a specific marker for ventricular chamber throughout chicken embryonic development and antisense vMLC2 treatment of primitive streak stage (stage 4) embryos to produce pronounced dilation of heart tube with severe...
Tipo: Journal article
Palavras-chave: Cardiogenesis; Cardiomyopathy; Myosin light chains; Tissue culture.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100009

Physiological behavior of bean (phaseolus vulgaris l.) Seedlings under metal stress
Provedor de dados: 48
Autores: Zengin,Fikriye.
The effects of nickel, cobalt, chromium and zinc on the content of vitamins A, E and C, malondialdehyde (MDA), chlorophyll and carotenoids were investigated in bean seedlings (Phaseolus vulgaris L.) grown in Hoagland solution Control and heavy metal-treated plants were grown for ten days in Hoagland solution. Vitamin A, E, and C content were measured in primary leaves by high performance liquid chromatographic (HPLC). MDA, chlorophyll and carotenoids were measured in leaves by spectrophotometer. In heavy metal treated plants, the levels of MDA, vitamins A, E and C and carotenoids significantly increased, while chlorophyll content decreased in leaves of seedlings. The results indicate that heavy metals caused an oxidative stress in bean plants. The...
Tipo: Journal article
Palavras-chave: Heavy metal; Vitamins A; E and C; MDA; Chlorophyll and carotenoids.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100012

The debate about death: an imperishable discussion?
Provedor de dados: 48
Autores: BACIGALUPO,FÉLIX; HUERTA,DANIELA; MONTEFUSCO-SIEGMUND,RODRIGO.
In this concise review we discuss some of the complex edges of the concept of death that arose after the notorious advances in science and medicine over the last 50 years, in which the classical cardio-pulmonary criteria have led to the neurological criteria of death. New complicated questions like the definition of death and the operational criteria for diagnosing it have arisen and we think that they are far from being adequately and satisfactorily solved. A number of important issues -like the reliability and differences between cardio-pulmonary versus brain based criteria of death, if death is an event or a process, the meaning of integration and irreversibility- have not yet received sufficient attention. Here we have approached the death problem from...
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500014

Assessment of changes in the brca2 and p53 genes in breast invasive ductal carcinoma in northeast Brazil
Provedor de dados: 48
Autores: Ramalho,Eduardo AVF; Silva-Filho,João LQ; Cartaxo,Marina FS; Cavalcanti,Carmelita BL; Rêgo,Moacyr JBM; Oliveira,Maria BM; Beltrão,Eduardo IC.
BACKGROUND: BRCA protein interacts with at least 13 different proteins that have been implicated with cancer susceptibility and loss of BRCA function is correlated to sensitivity to DNA crosslinking agents in preclinical models. RESULTS: BRCA2 methylation frequency was 44%, p53 Pro22 allele frequency was 32% and heterozygous frequency of Arg/Pro72 genotype was 60% which could be associated as risk factor for metastasis (p = 0.046 OR = 4.190). Regarding to polymorphism of codon 249 the frequency of Arg249 allele presented 82% which was considered not statistically significant. CONCLUSIONS: There was not statistical significance to BRCA2 promoter methylation with any parameters chosen. However, our findings suggest that patients who present heterozygous...
Tipo: Journal article
Palavras-chave: Breast cancer; Epigenetics; Metastasis; Methylation; Polymorphism.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100003

The beginning of life of a new human being from the scientific biological perspective and its bioethical implications
Provedor de dados: 48
Autores: Ventura-Juncá,Patricio; Santos,Manuel J.
The issue of when the human life begins is a very important subject since it has a significant impact on the decisions that we have to take in relation to human beings in development, particularly human embryos. In this article we discuss some of the more relevant biological evidence supporting the fact that beginning human life begins unquestionably at fertilization and the bioethical consequences.
Tipo: Journal article
Palavras-chave: Fertilization; Beginning of human life; Bioethics.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200013

Inositol 1,4,5-trisphosphate receptors in the heart
Provedor de dados: 48
Autores: MACKENZIE,LAUREN; RODERICK,H. LLEWELYN; PROVEN,ANDREW; CONWAY,STUART J; BOOTMAN,MARTIN D.
Inositol 1,4,5-trisphosphate (InsP3) is an established calcium-mobilizing messenger, which is well-known to activate Ca2+ signaling in many cell types. Contractile cardiomyocytes express hormone receptors that are coupled to the production of InsP3. Such cardioactive hormones, including endothelin, may have profound inotropic and arrhythmogenic actions, but it is unclear whether InsP3 underlies any of these effects. We have examined the expression and localization of InsP3 receptors (InsP3Rs), and the potential role of InsP3 in modulating cardiac excitation-contraction coupling (EC coupling). Stimulation of electrically-paced atrial and ventricular myocytes with a membrane-permeant InsP3 ester was found to evoke an increase in the amplitudes of action...
Tipo: Journal article
Palavras-chave: Calcium; Cardiac; Inositol; Arrhythmia; Ryanodine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400008

T-kininogen inhibits kinin-mediated activation of ERK in endothelial cells
Provedor de dados: 48
Autores: LEIVA-SALCEDO,ELIAS; PEREZ,VIVIANA; ACUÑA-CASTILLO,CLAUDIO; WALTER,ROBIN; SIERRA,FELIPE.
Serum levels of T-kininogen increase dramatically as rats approach the end of their lifespan. Stable expression of the protein in Balb/c 3T3 fibroblasts leads to a dramatic inhibition of cell proliferation, as well as inhibition of the ERK signaling pathway. T-kininogen is a potent inhibitor of cysteine proteinases, and we have described that the inhibition of ERK activity occurs, at least in part, via stabilization of the MAP kinase phosphatase, MKP-1. Since fibroblasts are not a physiological target of T-kininogen, we have now purified the protein from rat serum, and used it to assess the effect of T-kininogen on endothelial cells. Adding purified T-kininogen to EAhy 926 hybridoma cells resulted in inhibition of basal ERK activity levels, as estimated...
Tipo: Journal article
Palavras-chave: Aging; Endothelial cells; ERK pathway; Kininogen; Kinins.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200020

Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
Provedor de dados: 48
Autores: LIMA,ANDRÉA C PAULA; ARRIAGADA,CHRISTIAN; TORO,RODRIGO; CÁRDENAS,ANA MARÍA; CAVIEDES,RAÚL; FERREIRA,SERGIO T; CAVIEDES,PABLO.
We have previously characterized a number of small molecule organic compounds that prevent the aggregation of the &amp;#946;-amyloid peptide and its neurotoxicity in hippocampal neuronal cultures. We have now evaluated the effects of such compounds on amyloid precursor protein (APP) accumulation in the CTb immortalized cell line derived from the cerebral cortex of a trisomy 16 mouse, an animal model of Down's syndrome. Compared to a non-trisomic cortical cell line (CNh), CTb cells overexpress APP and exhibit slightly elevated resting intracellular Ca2+ levéis ([Ca2+]¡). Here, we show that the compounds 2,4-dinitrophenol, 3-nitrophenol and 4-anisidine decreased intracellular accumulation of APP in CTb cells. Those compounds were non-toxic to the cells, and...
Tipo: Journal article
Palavras-chave: Alzheimer's disease; Down syndrome; Intracellular amyloid; Murine trisomy 16; Small molecule inhibitors.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200001

Ion movements in cell death: from protection to execution
Provedor de dados: 48
Autores: FELIPE BARROS,LUIS; CASTRO,JOEL; BITTNER,CARLA X.
Cell death is preceded by severe disruption of inorganic ion homeostasis. Seconds to minutes after an injury, calcium, protons, sodium, potassium and chloride are exchanged between the cell and its environment. Simultaneously, ions are shifted between membrane compartments inside the cell, whereby mitochondria and endoplasmic reticulum play a crucial role. Depending of the type and severity of injury, two mutually exclusive metastable states can be reached, which predict the final outcome. Cells characterized by large increases in cytosolic [Ca2+], [Na+]; and [Mg2+] swell and die by necrosis; alternatively, cells characterized by high [H+]and low [K+], with normal [Na+] and normal to moderate [Ca2+] increases die by apoptosis. The levels of these ions...
Tipo: Journal article
Palavras-chave: Cell death; Membrane channels; Apoptosis; Necrosis.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200012

Caveolae and caveolae-like membrane domains in cellular signaling and disease: Identification of downstream targets for the tumor suppressor protein caveolin-1
Provedor de dados: 48
Autores: BENDER,FLORENT; MONTOYA,MARGARITA; MONARDES,VIRGINIA; LEYTON,LISETTE; QUEST,ANDREW F.G.
Caveolae are small, flask-shaped invaginations of the plasma membrane present on a large number of mammalian cells. Recent results obtained with knock-out mice for the gene caveolin-1 demonstrate that expression of caveolin-1 protein is essential for caveolae formation in vivo. Caveolae are implicated in a wide variety of cellular events including transcytosis, cholesterol trafficking and as cellular centers important in coordinating signalling events. Caveolae share this role and the property of detergent insolubility with plasma membrane assemblies rich in glycosphingolipids and cholesterol, often called lipid rafts, but preferably referred to here as caveolae-like membrane domains. Due to such widespread presence and usage in cellular function, caveolae...
Tipo: Journal article
Palavras-chave: Caveolae; Caveolae-like domains; Lipid rafts; Caveolin-1; Tumor suppressor; Colon carcinoma cells; Microarray analysis.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200006

Discrepancy variation of dinucleotide microsatellite repeats in eukaryotic genomes
Provedor de dados: 48
Autores: GAO,HUAN; CAI,SHENGLI; YAN,BINLUN; CHEN,BAIYAO; YU,FEI.
To address whether there are differences of variation among repeat motif types and among taxonomic groups, we present here an analysis of variation and correlation of dinucleotide microsatellite repeats in eukaryotic genomes. Ten taxonomic groups were compared, those being primates, mammalia (excluding primates and rodentia), rodentia, birds, fish, amphibians and reptiles, insects, molluscs, plants and fungi, respectively. The data used in the analysis is from the literature published in the Journal of Molecular Ecology Notes. Analysis of variation reveals that there are no significant differences between AC and AG repeat motif types. Moreover, the number of alleles correlates positively with the copy number in both AG and AC repeats. Similar conclusions...
Tipo: Journal article
Palavras-chave: Microsatellites; Eukaryotic genomes; Variation of SSR; Copy number; Meta-analysis.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300011

Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
Provedor de dados: 48
Autores: Marthandan,Shiva; Menzel,Uwe; Priebe,Steffen; Groth,Marco; Guthke,Reinhard; Platzer,Matthias; Hemmerich,Peter; Kaether,Christoph; Diekmann,Stephan.
BACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determined the transcriptional changes combined with the transition into replicative senescence in primary human fibroblast strains. Here, by &amp;#947;-irradiation we induced premature cellular senescence in the fibroblast cell strains (HFF and MRC-5) and determined the corresponding transcriptional changes by high-throughput RNA sequencing. RESULTS: Comparing the transcriptomes, we found a high degree of similarity in differential gene expression in replicative as well...
Tipo: Journal article
Palavras-chave: Senescence Fibroblasts &amp;#947; -irradiation Aging Transcriptome analysis DNA repair.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100034

CONTRIBUCIÓN DE LAS NORMAS CIOMS 2002 AL DESARROLLO BIOTECNOLÓGICO: LA CORRESPONSABILIZACIÓN DEL CIENTÍFICO
Provedor de dados: 48
Autores: BOTA ARQUÉ,ALEXANDRE.
La revolución biotecnológica afronta grandes retos, entre ellos la participación social, la bioseguridad y la protección tanto del germoplasma como del conocimiento ancestral que de él se derivan. Para afrontar estos dilemas se plantea la necesidad de buscar una corresponsabilización de todos los actores del desarrollo biotecnológico. En este artículo se realiza un correlato partiendo de la reflexión ya iniciada por la comunidad médica con sus pautas éticas internacionales para la investigación biomédica en seres humanos, CIOMS, versus la biotecnología para buscar una corresponsabilización de los tecnocientíficos, biotecnólogos en sus investigaciones con la pretensión utópica de buscar un desarrollo biotecnológico mas respetuoso con el hombre y con su...
Tipo: Journal article
Palavras-chave: Bioética; Biotecnología; Empoderamiento.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200005

Anatomical root variations in response to water deficit: wild and domesticated common bean (Phaseolus vulgaris L)
Provedor de dados: 48
Autores: Peña-Valdivia,Cecilia B; Sánchez-Urdaneta,Adriana B; Meza Rangel,Joel; Juárez Muñoz,Juana; García-Nava,Rodolfo; Celis Velázquez,Raquel.
Root anatomical responses to water deficit are diverse and regulation of water uptake strongly depends on plant anatomy. The ancestors of common bean (Phaseolus vulgaris L.) cultivars are the wild common beans. Because wild beans adapt and survive well in theon atural environment, it is hypothesized that wild common bean roots are less affected than those of domesticated beans at low substrate water potential (&amp;#968;W). A wild common bean accession from Chihuahua Mexico and cv. Bayomex were studied. Seedlings with a mean root length between 3 and 4 cm were maintained for 24 h in vermiculite at &amp;#968;W of -0.03 (well hydrated), -0.65, -1.48 and -2.35 MPa (partially dry). Ten anatomical characteristics of differentiation and cell division in root...
Tipo: Journal article
Palavras-chave: Endodermis; Protoderm; Seedlings; Water potential; Xylem.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400006

Nitric oxide and carotid body chemoreception
Provedor de dados: 48
Autores: ITURRIAGA,RODRIGO.
Nitric oxide (NO) has been proposed as an inhibitory modulator of carotid body chemosensory responses to hypoxia. It is believed that NO modulates carotid chemoreception by several mechanisms, which include the control of carotid body vascular tone and oxygen delivery and reduction of the excitability of chemoreceptor cells and petrosal sensory neurons. In addition to the well-known inhibitory effect, we found that NO has a dual (dose-dependent) effect on carotid chemoreception depending on the oxygen pressure level. During hypoxia, NO is primarily an inhibitory modulator of carotid chemoreception, while in normoxia NO increased the chemosensory activity. This excitatory effect produced by NO is likely mediated by an impairment of mitochondrial electron...
Tipo: Journal article
Palavras-chave: Carotid body; Chemoreceptor; Cytochrome oxidase; Hypoxia; Nitric oxide; Oxidative phosphorylation.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200019

tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori
Provedor de dados: 48
Autores: Cerda,Oscar A; Núñez-Villena,Felipe; Soto,Sarita E; Ugalde,José Manuel; López-Solís,Remigio; Toledo,Héctor.
About half of the human population is infected with Helicobacter pylori, a bacterium causing gastritis, peptic ulcer and progression to gastric cancer. Chemotaxis and flagellar motility are required for colonization and persistence of H. pylori in the gastric mucus layer. It is not completely clear which chemical gradients are used by H. pylori to maintain its position. TlpA, a chemotaxis receptor for arginine/ bicarbonate, has been identified. This study aimed to find out whether tlpA gene expression is required for the chemotactic response to arginine/bicarbonate. Wild-type motile H. pylori ATCC 700392 and H. pylori ATCC 43504, a strain having an interrupted tlpA gene, were used. Also, a tlpA-knockout mutant of H. pylori 700392 (H. pylori 700-tlpA::cat)...
Tipo: Journal article
Palavras-chave: TlpA; Chemotaxis; Helicobacter pylori; Arginine; Bicarbonate.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300009

XLIV REUNION ANUAL DE LA SOCIEDAD DE BIOLOGIA DE CHILE: XXXIV REUNION ANUAL DE LA SOCIEDAD DE GENETICA DE CHILE XXIII REUNION ANUAL DE LA SOCIEDAD DE FARMACOLOGIA DE CHILE XVI REUNION ANUAL DE LA SOCIEDAD CHILENA DE CIENCIAS FISIOLOGICAS
Provedor de dados: 48
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300012

Tissue mRNA expression in rat of newly described matrix metalloproteinases
Provedor de dados: 48
Autores: BERNAL,FABIÁN; HARTUNG,HANS-PETER; KIESEIER,BERND C.
Matrix metalloproteinases (MMPs) comprise a large group of endoproteinases that degrade all protein components of the extracellular matrix. Functionally, MMPs contribute to several different physiological as well as pathological conditions. The number of newly described MMPs has increased in recent years, although current knowledge about their expression pattern in various tissues remains incomplete. Here we analyzed the relative mRNA expression of the most recently described MMPs _ MT5-MMP (MMP-24), MT6-MMP (MMP-25), MMP-27 and epilysin (MMP-28) _ in a broad selection of rat tissues using real time-PCR. MMP-24 mRNA was found to be widely expressed with predominance in the central nervous system. MMP-25 mRNA, in contrast, exhibited peak expression levels...
Tipo: Journal article
Palavras-chave: Bone; CNS; Epilysin; Kidney; MT5-MMP; MT6-MMP; Testis.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200016

Typical and atypical stem cells in the brain, vitamin C effect and neuropathology
Provedor de dados: 48
Autores: Nualart,Francisco; Salazar,Katterine; Oyarce,Karina; Cisternas,Pedro; Jara,Nery; Silva-Álvarez,Carmen; Pastor,Patricia; Martínez,Fernando; García,Andrea; García-Robles,María de los Ángeles; Tapia,Juan Carlos.
Stem cells are considered a valuable cellular resource for tissue replacement therapies in most brain disorders. Stem cells have the ability to self-replicate and differentiate into numerous cell types, including neurons, oligodendrocytes and astrocytes. As a result, stem cells have been considered the "holy grail" of modern medical neuroscience. Despite their tremendous therapeutic potential, little is known about the mechanisms that regulate their differentiation. In this review, we analyze stem cells in embryonic and adult brains, and illustrate the differentiation pathways that give origin to most brain cells. We also evaluate the emergent role of the well known anti-oxidant, vitamin C, in stem cell differentiation. We believe that a complete...
Tipo: Journal article
Palavras-chave: Stem cells; Radial glia; Vitamin C; SVCT2; Brain development; Tanycytes; Bergmann glia; Neurogenic niche; Type B cells; Neuroblast; Parkinson's disease; Alzheimer's disease; Amyotrophic lateral sclerosis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300006

Rafael Vicuña es testigo experto en importante juicio internacional sobre patentes
Provedor de dados: 48
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100005

Occurrence of killer yeast strains in industrial and clinical yeast isolates
Provedor de dados: 48
Autores: BAEZA,MARCELO E; SANHUEZA,MARIO A; CIFUENTES,VÍCTOR H.
The secretion of proteinaceous toxins is a widespread characteristic in environmental and laboratory yeast isolates, a phenomenon called "killer system". The killer phenotype (K+) can be encoded by extrachromosomal genetic elements (EGEs) as double stranded DNA or RNA molecules (dsDNA, dsRNA) or in nuclear genes. The spectrum of action and the activity of killer toxins are influenced by temperature, salinity and pH of media. In the present work we determined the existence of K+ in a collection of S. cerevisiae and P. anómala yeasts isolated from environmental, industrial and clinical sources. The assays were performed in strains belonging to three yeast genera used as sensitive cells and under a wide range of pH and temperatures. Approximately 51 % of...
Tipo: Journal article
Palavras-chave: Killer system; Mycotoxin; DsRNA.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200007

Septin 7: Actin cross-organization is required for axonal association of Schwann cells
Provedor de dados: 48
Autores: Roth,Alejandro D; Liazoghli,Dalinda; Perez De Arce,Felipe; Colman,David R.
Myelin sheaths present two distinct domains: compacted myelin spirals and flanking non-compacted cytoplasmic channels, where lipid and protein segregation is established by unknown mechanisms. Septins, a conserved family of membrane and cytoskeletal interacting GTPases, form intracellular diffusion barriers during cell division and neurite extension and are expressed in myelinating cells. Septins, particularly septin 7 (Sept7), the central constituent of septin polymers, are associated with the cytoplasmic channels of myelinating cells. Here we show that Schwann cells deprived of Sept7 fail to wrap around axons from dorsal root ganglion neurons and exhibit disorganization of the actin cytoskeleton. Likewise, Sept7 distribution is dependent on microfilament...
Tipo: Journal article
Palavras-chave: Axon wrapping; Septins; Myelination; Schwann Cells.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300004

A low-protein diet during pregnancy prevents modifications in intercellular communication proteins in rat islets
Provedor de dados: 48
Autores: Marçal-Pessoa,Ana Flávia; Bassi-Branco,Carmen Lucia; Salvatierra,Cristiana dos Santos Barbosa; Stoppiglia,Luiz Fabrizio; Ignacio-Souza,Letícia Martins; Reis,Sílvia Regina de Lima; Veloso,Roberto Vilela; Reis,Marise Auxiliadora de Barros; Carneiro,Everardo Magalhães; Boschero,Antonio Carlos; Arantes,Vanessa Cristina; Latorraca,Márcia Queiroz.
^len
Tipo: Journal article
Palavras-chave: Connexin 36 Connexin 43 &amp;#946; -catenin Low protein diet Pregnancy Pancreatic islets Rat.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100003

Misconceptions and false expectations in neutral evolution
Provedor de dados: 48
Autores: VALENZUELA,CARLOS Y.
Neutral evolution results from random recurrent mutation and genetic drift. A small part of random evolution, that which is related to protein or DNA polymorphisms, is the subject of the Neutral Theory of Evolution. One of the foundations of this theory is the demonstration that the mutation rate (m) is equal to the substitution rate. Since both rates are independent of population size, they are independent of drift, which is dependent upon population size. Neutralists have erroneously equated the substitution rate with the fixation rate, despite the fact that they are antithetical conceptions. The neutralists then applied the random walk stochastic model to justify alleles or bases that were fixated or eliminated. In this model, once the allele or base...
Tipo: Journal article
Palavras-chave: Neutral evolution; Neutral theory; Misconceptions; Bases fixation; Elimination.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300004

In vitro antibacterial activity in seed extracts of Manilkara zapota, Anona squamosa, and Tamarindus indica
Provedor de dados: 48
Autores: Kothari,Vijay; Seshadri,Sriram.
Extracts prepared from seeds of Manilkara zapota, Anona squamosa, and Tamarindus indica were screened for their antibacterial activity by disc diffusion and broth dilution methods. Acetone and methanol extracts of T. indica seeds were found active against both gram-positive and gram-negative organisms. MIC values of potent extracts against susceptible organisms ranged from 53-380 &amp;#956;g/mL. Methanol extract of T. indica and acetone extract of M. zapota seeds were found to be bactericidal.
Tipo: Journal article
Palavras-chave: Tamarindus indica; Manilkara zapota; In vitro activity; Bactericidal activity.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200003

In vitro fertilization (IVF) in mammals: epigenetic and developmental alterations. Scientific and bioethical implications for IVF in humans
Provedor de dados: 48
Autores: Ventura-Juncá,Patricio; Irarrázaval,Isabel; Rolle,Augusto J; Gutiérrez,Juan I; Moreno,Ricardo D; Santos,Manuel J.
The advent of in vitro fertilization (IVF) in animals and humans implies an extraordinary change in the environment where the beginning of a new organism takes place. In mammals fertilization occurs in the maternal oviduct, where there are unique conditions for guaranteeing the encounter of the gametes and the first stages of development of the embryo and thus its future. During this period a major epigenetic reprogramming takes place that is crucial for the normal fate of the embryo. This epigenetic reprogramming is very vulnerable to changes in environmental conditions such as the ones implied in IVF, including in vitro culture, nutrition, light, temperature, oxygen tension, embryo-maternal signaling, and the general absence of protection against foreign...
Tipo: Journal article
Palavras-chave: In vitro fertilization (IVF) in mammals; Epigenetics; Developmental alterations; Bioethical implications.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100068

Biological properties of the Chilean native moss Sphagnum magellanicum
Provedor de dados: 48
Autores: MONTENEGRO,GLORIA; PORTALUPPI,MARIANA C; SALAS,FRANCISCO A; DÍAZ,MARÍA F.
An ethanol extract prepared from the gametophyte Chilean native moss Sphagnum magellanicum was dried out, weighed and dissolved in distilled water. This extract was then assayed for its antibacterial activity against the G(-) bacteria Azotobacter vinelandii, Erwinia carotovora subsp. carotovora, Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, and the G(+) bacteria Staphylococcus aureus subsp. aureus, and Streptococcus type b. The growth of the cultures of E. carotovora subsp. carotovora, and V. cholerae was inhibited at a concentration of 581mg/ml of extract, while the cultures of E. coli, S. typhi and Streptococcus type b were inhibited at a concentration of 1.16 mg/mL of extract. The concentration of...
Tipo: Journal article
Palavras-chave: Sphagnum magellanicum; Phenolic compounds; Antibacterial activity; MIC.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200012

The interaction of ryanoids with individual ryanodine receptor channels
Provedor de dados: 48
Autores: WILLIAMS,ALAN J; TANNA,BHAVNA.
Ryanodine binds with high affinity and specificity to a class of Ca2+-release channels known as ryanodine receptors (RyR). The interaction with RyR results in a dramatic alteration in function with open probability (Po) increasing markedly and rates of ion translocation modified. We have investigated the features of ryanodine that govern the interaction of the ligand with RyR and the mechanisms underlying the subsequent alterations in function by monitoring the effects of congeners and derivatives of ryanodine (ryanoids) on individual RyR2 channels. While the interaction of all tested ryanoids results in an increased Po, the amplitude of the modified conductance state depends upon the structure of the ryanoid. We propose that different rates of cation...
Tipo: Journal article
Palavras-chave: Calcium; Ca2+-release channel; Sarcoplasmic reticulum; Ryanodine; Single channel.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400006

Genomic imprinting and human chromosome 15
Provedor de dados: 48
Autores: REPETTO,GABRIELA M.
Genomic imprinting is a reversible phenomenon that affects the expression of genes depending on their parental origin. The best characterized human disorders resulting from an alteration of the imprinting process are Angelman and Prader-Willi syndromes. They are due to the lack of active maternal or paternal genes, respectively, from chromosome region 15q11q13. Most cases arise via interstitial deletions. We review evidence that other common cytogenetic alterations of this region, interstitial and supernumerary duplications, could be the reciprocal products of the deletions and are also affected by the imprinting phenomenon, given the predominance of maternally-derived duplications in patients ascertained due to developmental delays or autistic features.
Tipo: Journal article
Palavras-chave: Chromosome 15; Chromosome 15 deletions/duplications; Genomic imprinting.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200020

Elevated yield of Monacolin K in Monascus purpureus by fungal elicitor and mutagenesis of UV and LiCl
Provedor de dados: 48
Autores: Sun,Jia-Long; Zou,Xiao; Liu,Ai-Ying; Xiao,Tang-Fu.
In China, Monascus spp., a traditional fungus used in fermentation, is used as a natural food additive. Monascus spp. can produce a secondary metabolite, monacolin K namely, which is proven to be a cholesterol-lowering and hypotensive agent. Hence, recently, many researchers have begun focusing on how to increase the production of monacolin K by Monascus purpureus. In the present study, we investigated the effect of the fungal elicitor and the mutagenesis of UV &amp;amp; LiCl on the amount of monacolin K produced by Monascus purpureus. The fugal elicitor, Sporobolomyces huaxiensis, was isolated from tea leaves and its filtrate was added into the culture filtrate of Monascus purpureus during growth to induct the production of monacolin K. The results showed...
Tipo: Journal article
Palavras-chave: Monascus spp.; Monacolin K; Fungal elicitor; Mutagenesis.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400010

Electrolyzed-reduced water increases resistance to oxidative stress, fertility, and lifespan via insulin/IGF-1-like signal in C. elegans
Provedor de dados: 48
Autores: Park,Seul-Ki; Park,Sang-Kyu.
Electrolyzed-reduced water (ERW) scavenges reactive oxygen species and is a powerful anti-oxidant. A positive correlation between oxidative stress and aging has been proved in many model organisms. In Caenorhabditis elegans, many long-lived mutants showed reduced fertility as a trade off against longevity phenotype. We aimed to study the effect of ERW on oxidative stress, fertility and lifespan of C. elegans. We also investigated the genetic pathway involved in the effect of ERW on resistance to oxidative stress and lifespan. We compared lifespan and fertility of worms in media prepared with distilled water and ERW. ERW significantly extended lifespan and increased the number of progeny produced. Then the effect of ERW on resistance to oxidative stress and...
Tipo: Journal article
Palavras-chave: Electrolyzed-reduced water; Aging; Daf-16; Fertility; Lifespan.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200005

Ontogenetic changes in the fractal geometry of the bronchial tree in Rattus norvegicus
Provedor de dados: 48
Autores: CANALS,MAURICIO; OLIVARES,RICARDO; LABRA,FABIAN; NOVOA,FRANCISCO F.
Respiration and metabolism change dramatically over the course of the development of vertebrates. In mammals these changes may be ascribed to organogenesis and differentiation of structures involved in gas exchange and transport and the increase in size. Since young as well as mature individuals must be well-designed if the species is to survive, the physiological changes during the development should be matched with geometrical or structural adjustments of the respiratory system. The aim of this study was to evaluate changes in the fractal geometry of the bronchial tree during the postnatal development of the rat.  The average fractal dimension of the bronchial tree of the rats was 1.587, but that of juveniles was larger than that of the adults. We found...
Tipo: Journal article
Palavras-chave: Bronchial tree; Fractals; Ontogeny.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100010

Ciliary Neurotrophic Factor (CNTF) affects the excitable and contractile properties of innervated skeletal muscles
Provedor de dados: 48
Autores: RAMIREZ,BEATRIZ U; RETAMAL,LEIRA; VERGARA,CECILIA.
The well-established trophic role of CNTF upon neurons led to performing clinical trials in patients of neurodegenerative diseases. However, trials were suspended due to side effects such as severe weight loss, hyperalgesia, coughing, muscle cramps and pain. So far it is not known how CNTF triggers the problems related to skeletal muscle cramps and pain. CNTF has also been described as a myotrophic factor for denervated skeletal muscles, but the possibility that it affects innervated muscles has also been considered. Since a myotrophic factor could be a valuable tool for treatment of several muscle diseases, we studied the effects of low doses of CNTF delivered systemically by an osmotic pump, over the electrical and mechanical properties of innervated and...
Tipo: Journal article
Palavras-chave: CNTF; Contraction and relaxation time; Denervation; EMG; Fibrillation; Skeletal muscle.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300003

Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats
Provedor de dados: 48
Autores: HATORI,EIKI; SAKURABA,SHIGEKI; KASHIWAGI,MASANORI; KURIBAYASHI,JUNYA; TSUJITA,MIKI; HOSOKAWA,YUKI; TAKEDA,JUNZO; KUWANA,SHUN-ICHI.
Nicotine exposure is a risk factor in several breathing disorders Nicotinic acetylcholine receptors (nAChRs) exist in the ventrolateral medulla, an important site for respiratory control. We examined the effects of nicotinic acetylcholine neurotransmission on central respiratory control by addition of a nAChR agonist or one of various antagonists into superfusion medium in the isolated brainstem-spinal cord from neonatal rats. Ventral C4 neuronal activity was monitored as central respiratory output, and activities of respiratory neurons in the ventrolateral medulla were recorded in whole-cell configuration. RJR-2403 (0.1-10mM), alpha4beta2 nAChR agonist induced dose-dependent increases in respiratory frequency. Non-selective nAChR antagonist mecamylamine...
Tipo: Journal article
Palavras-chave: Brainstem-spinal cord; Neonatal rat; Inspiratory neuron; Preinspiratory neuron; Nicotinic acetylcholine receptor.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200014

Differences in lipogenesis and lipolysis in obese and non-obese adult human adipocytes
Provedor de dados: 48
Autores: CIFUENTES,MARIANA; ALBALA,CECILIA; ROJAS,CECILIA V.
It has been proposed that differences in adipocyte function and/or metabolism between obese and lean individuáis may manifest themselves in functional adipose tissue abnormalities that lead to metabolic disorders in obesity. We studied lipogenesis and lipolysis of omental adipocytes from obese (OB) and non-obese (NOB) humans. The specific activity of the lipogenic marker enzyme G3PDH was 50% lower in total adipocytes of OB compared to that of NOB subjects. Omental adipocytes from OB subjects also had lower basal lipolytic levéis, and a lower lipolytic response to p-adrenergic stimulus. Cholesterol depletion of adipocyte plasma membrane using methyl &amp;#946;-cyclodextrin caused a lipolytic effect on adipocytes of both groups together, but when obese and...
Tipo: Journal article
Palavras-chave: Adipocyte; Cholesterol; Lipogenesis; Lipolysis; Obesity; Triglyceride metabolism.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200009

The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
Provedor de dados: 48
Autores: FIUZA,TATIANA S; SILVA,PAULO C; PAULA,JOSÉ R; TRESVENZOL,LEONICE M F; SABÓIA-MORAIS,SIMONE M T.
Hyptidendron canum (Pohl ex Benth.) Harley is a native tree of the Brazilian Savannah. The fish Oreochromis niloticus L. was used as an experimental model to determine the bioactivity of the crude ethanol extract as well as ethyl acetate, hexanic and chloroform fractions obtained from its leaves. The plant ethanol extract and fractions were administered to the fish orally with their feed. Twenty four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative...
Tipo: Journal article
Palavras-chave: Fish; Nile tilapia; Savannah biome; Vasodilation.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200003

Wnt signaling: A complex issue
Provedor de dados: 48
Autores: HENDRIKS,BART; REICHMANN,ERNST.
The development of tissues and organs in multicellular organisms is controlled by the interplay of several signaling pathways that cross-talk to provide positional information and induce cell fate specification. Together with other families of secreted factors such as TGFßs, FGFs, Hedgehog and Notch proteins, Wnt growth factors are crucially implicated in these processes. Here, we will first discuss molecular mechanisms and then consider some biological consequences of Wnt signaling
Tipo: Journal article
Palavras-chave: Wnts; Frizzled; LRP 5/6; SS-catenin; APC; TCF.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200019

Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro
Provedor de dados: 48
Autores: RAHMAN,ASIM; ALAM,MAHMOOD; RAO,SUDHA; CAI,LIN; LUTHER T.,CLARK; SHAFIQ,SAYID; SIDDIQUI,M.A.Q.
Doxorubicin (Dox) is a potent anti-cancer agent with cardiotoxic side-effects but the mechanism of its cardiotoxicity and its effect on expression of the vasoactive atrial natriuretic peptide (ANP), an important marker for cardiac hypertrophy, are little understood. The present study examined Dox-induced changes in vivo in hearts of 6 mongrel dogs and 5 Sprague-Dawley rats and in vitro in cardiac cultures of neonatal rats. Quantitative RT-PCR analysis using g32-p labeled primers for ß-actin, phospholamban (PLB) and ANP showed a selective 5-fold increase of ANP mRNA in Dox-treated dog hearts in comparison to controls. Similarly, northern analysis of GAPD, ß-actin, cardiac a-actin and ANP gave a selective 4.5-fold increase in ANP transcripts in Dox-treated...
Tipo: Journal article
Palavras-chave: Atrial natriuretic peptide; Gene expression; Adriamycin; Heart failure.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300007

Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients
Provedor de dados: 48
Autores: Vicuña,Lucas; Pardo,Evelyn; Curkovic,Cristóbal; Doger,Remziye; Oyanadel,Claudia; Metz,Claudia; Massardo,Loreto; González,Alfonso; Soza,Andrea.
Galectin-8 belongs to a family of mammalian lectins that recognize glycoconjugates present on different cell surface components and modulate a variety of cellular processes. A role of Gal-8 in the immune system has been proposed based on its effects in immune cells, including T and B lymphocytes, as well as the presence of anti-Gal-8 autoantibodies in the prototypic autoimmune disease systemic lupus erythematosus (SLE). We have previously described that Gal-8 induces apoptosis in activated T cells interacting with certain &amp;#946;1 integrins and this effect is counteracted by the anti-Gal-8 autoantibodies. Given that Gal-8 can potentially interact with several glycoproteins, here we analyzed the &amp;#946;2 integrin Lymphocyte Function-Associated...
Tipo: Journal article
Palavras-chave: Galectin-8; LFA-1; ICAM-1; SLE; Autoantibodies.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300008

Spatial cognition and memory: a reversible lesion with lidocaine into the anteromedial/posterior parietal cortex (AM/PPC) affects differently working and long-term memory on two foraging tasks
Provedor de dados: 48
Autores: ESPINA-MARCHANT,PABLO; PINTO-HAMUY,TERESA; BUSTAMANTE,DIEGO; MORALES,PAOLA; ROBLES,LUIS; HERRERA-MARSCHITZ,MARIO.
Place memory is relevant for exploration and forage behaviour. When food supply is dispersed, a win-shift has advantage over a win-stay strategy. In the Olton Octagonal Maze, the rat follows a win-shift strategy using working memory. However, in the Olton 4x4 version, the rat follows a win-stay strategy, using both working and long-term memories. It has been suggested that the neocortex is required for the resolution of tasks demanding long-term, but not for that demanding working memory alone. The role of anteromedial/posterior parietal cortex (AM/PPC) was investigated here, using a reversible lesion induced by intracerebral lidocaine infusion. Long-Evans rats were implanted with guide cannulae into the AM/PPC and trained in an Olton 4x4 maze, counting...
Tipo: Journal article
Palavras-chave: Anteromedial/posterior parietal cortex hippocampus lidocaine; Long-term memory working memory intracerebral infusion rat.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500003

Chronobiologic blood pressure assessment: Maturation of the daily rhythm in newborn foals
Provedor de dados: 48
Autores: PICCIONE,GIUSEPPE; ASSENZA,ANNA; FAZIO,FRANCESCO; GIANNETTO,CLAUDIA; CAOLA,GIOVANNI.
Circadian rhythmicity of physiological processes in animals has been described for some variables. In order to investigate the daily rhythmicity of blood pressure, seven foals (Equus caballus) were used for 40 days after birth. Measurements of blood pressure were done by means of an oscillometric apparatus (Argus TM-7, Schiller, Barr Switzerland) with the foals in a standing position and the cuff placed around the tail. Blood pressure was recorded twice a day, 1 hour before dawn and 1 hour before dusk for the first 10 days of life, every 2 days from the 11th to the 32nd day, on the 36th and on the 40th day. Two-way repeated measures analysis of variance (ANOVA) were used to determine statistically significant differences between the mean values recorded at...
Tipo: Journal article
Palavras-chave: Blood pressure; Circadian rhythm; Chronophisiology; Foals; Equus caballus.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100007

DR. JORGE ALLENDE NUEVO MIEMBRO DE LA ACADEMIA DE CIENCIAS DE ESTADOS UNIDOS
Provedor de dados: 48
Autores: ORELLANA,OMAR.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200004

Phenolic compounds and vitamins in wild and cultivated apricot (Prunus armeniaca L.) fruits grown in irrigated and dry farming conditions
Provedor de dados: 48
Autores: Kan,Tuncay; Gundogdu,Muttalip; Ercisli,Sezai; Muradoglu,Ferhad; Celik,Ferit; Gecer,Mustafa Kenan; Kodad,Ossama; Zia-Ul-Haq,Muhammad.
BACKGROUND: Turkey is the main apricot producer in the world and apricots have been produced under both dry and irrigated conditions in the country. In this study, phenolic compounds and vitamins in fruits of one wild (Zerdali) and three main apricot cultivars ('Cataloglu', 'Hacihaliloglu' and 'Kabaasi') grown in both dry and irrigated conditions in Malatya provinces in Turkey were investigated. RESULTS: The findings indicated that higher content of phenolic compounds and vitamins was found in apricot fruits grown in irrigated conditions. Among the cultivars, 'Cataloglu' had the highest rutin contents both in irrigated and dry farming conditions as 2855 µg in irrigated and 6952 µg per 100 g dried weight base in dry conditions and the highest chlorogenic...
Tipo: Journal article
Palavras-chave: Apricot; Phytochemicals; Ripening; Irrigation; HPLC.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100046

Analysis of the intronic single nucleotide polymorphism rs#466452 of the nephrin gene in patients with diabetic nephropathy
Provedor de dados: 48
Autores: GONZÁLEZ,RODRIGO; TIRADO,ARES; ROJAS,LUIS A; OSSANDÓN,FRANCISCO J; ALVO,MIRIAM; WOLFF,CARLOS; SEELENFREUND,DANIELA; DURRUTY,PILAR; LOBOS,SERGIO.
We present the analysis of an intronic polymorphism of the nephrin gene and its relationship to the development of diabetic nephropathy in a study of diabetes type 1 and type 2 patients. The frequency of the single nucleotide polymorphism rs#466452 in the nephrin gene was determined in 231 patients and control subjects. The C/T status of the polymorphism was assessed using restriction enzyme digestions and the nephrin transcript from a kidney biopsy was examined. Association between the polymorphism and clinical parameters was evaluated using multivaríate correspondence analysis. A bioinformatics analysis of the single nucleotide polymorphism rs#466452 suggested the appearance of a splicing enhancer sequence in intron 24 of the nephrin gene and a...
Tipo: Journal article
Palavras-chave: Diabetic nephropathy; NPHS1; Polymorphism; Splicing enhancer.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200007

Endothelial cell oxidative stress and signal transduction
Provedor de dados: 48
Autores: FONCEA,ROCIO; CARVAJAL,CRISTIAN; ALMARZA,CAROLINA; LEIGHTON,FEDERICO.
Endothelial dysfunction (ED) is an early event in atherosclerotic disease, preceding clinical manifestations and complications. Increased reactive oxygen species (ROS) have been implicated as important mechanisms that contribute to ED, and ROS’s may function as intracellular messengers that modulate signaling pathways. Several intracellular signal events stimulated by ROS have been defined, including the identification of two members of the mitogen activated protein kinase family (ERK1/2 and big MAP kinase, BMK1), tyrosine kinases (Src and Syk) and different isoenzymes of PKC as redox-sensitive kinases. ROS regulation of signal transduction components include the modification in the activity of transcriptional factors such as NFkB and others that result in...
Tipo: Journal article
Palavras-chave: Oxidative stress; Signal transduction; Gene expression; Endothelial cell; Atherosclerosis; Antioxidants.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200008

Gamete interactions in teleost fish: the egg envelope. Basic studies and perspectives as environmental biomonitor
Provedor de dados: 48
Autores: Berois,Nibia; Arezo,María J; Papa,Nicolás G.
The current knowledge about teleost fish egg envelope is summarized. The paper analyzes the organization and deposition process of the protein composition and genes involved in the synthesis of teleost fish egg envelopes and their role in gamete interaction during fertilization. Pelagic and demersal species that our research group is working with are especially considered. The vertebrate ZP family of proteins, the evolution and relationship among the different genes and their expression are taken into account. We consider fish envelope as a possible biomonitor for ecological contaminants. The biotechnological applications for aquaculture and genomic and post-genomic approaches are auspicious.
Tipo: Journal article
Palavras-chave: Fish egg envelope; ZP genes.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200002

The functional links between prion protein and copper
Provedor de dados: 48
Autores: VARELA-NALLAR,LORENA; TOLEDO,ENRIQUE M; CHACÓN,MARCELO A; INESTROSA,NIBALDO C.
Prion diseases are fatal neurodegenerative disorders associated with the conversion of the cellular prion protein (PrPC) into a pathologic isoform. Although the physiological function of PrPC remains unknown, evidence relates PrPC to copper metabolism and oxidative stress as suggested by its copper-binding properties in the N-terminal octapeptide repeat region. This region also reduces copper ions in vitro, and this reduction ability is associated with the neuroprotection exerted by the octarepeat region against copper in vivo. In addition, the promoter region of the PrPC gene contains putative metal response elements suggesting it may be regulated by heavy metals. Here we address some of the evidence that support a physiological link between PrPC and...
Tipo: Journal article
Palavras-chave: Prion protein; Copper; Heparin; HSPG; Prnp; MRE.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100005

DNA damage and related modifier genes in Italian Cystic fibrosis patients
Provedor de dados: 48
Autores: STERPONE,SILVIA; CORNETTA,TOMMASO; ANGIONI,ADRIANO; FISCARELLI,ERSILIA; LUCIDI,VINCENZINA; TESTA,ANTONELLA; COZZI,RENATA.
Cystic Fibrosis (CF) is an autosomal recessive multisystemic disorder showing a highly heterogeneous phenotype, even among siblings carrying identical CFTR mutations. Moreover, oxidative stress is of central importance in the pathogenesis of cystic fibrosis. The present study seeks to value the presence of oxidative damage in CF patients and the possible modifier effect of repair and glutathione-S-transferase genes. We analysed the presence of DNA damage in leukocytes of 63 CF patients at an Italian CF centre and 63 controls, through the alkaline Comet assay to detect DNA strand breaks. Furthermore, controls and 93 CF subjects were genotyped for 5 genes by RFLP-PCR (XRCC1,0GG1,GSTP1) and PCR assay (GSTM1, GSTT1). No difference in Comet assay values was...
Tipo: Journal article
Palavras-chave: Comet assay; Cystic fibrosis; DNA damage; Genetic polymorphisms; Modifier genes.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400009

Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro
Provedor de dados: 48
Autores: Eroglu,Sema; Pandir,Dilek; Uzun,Fatma G; Bas,Hatice.
Organophosphate (OP) pesticides such as dichlorvos (DDVP) intoxication has been shown to produce oxidative stress due to the generation of free radicals, which alter the antioxidant defense system in erythrocytes. In this study, the effects of DDVP (1, 10, 100 µM) or DDVP + vitamin C (VC; 10 µM) or vitamin E (VE; 30 µM), on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in human erythrocytes were examined in vitro. There were no statistical differences between all groups for 1 µM concentration of DDVP. Treatment with DDVP alone produced an increase in the level of MDA and decreased activities of antioxidant enzymes (P &lt; 0.05). Groups treated with vitamins and DDVP showed...
Tipo: Journal article
Palavras-chave: Antioxidant enzyme; Dichlorvos; Oxidative stress; Vitamin; Malondialdehyde.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100005

Glucose transporters: expression, regulation and cancer
Provedor de dados: 48
Autores: MEDINA,RODOLFO A.; OWEN,GARETH I.
Mammalian cells depend on glucose as a major substrate for energy production. Glucose is transported into the cell via facilitative glucose transporters (GLUT) present in all cell types. Many GLUT isoforms have been described and their expression is cell-specific and subject to hormonal and environmental control. The kinetic properties and substrate specificities of the different isoforms are specifically suited to the energy requirements of the particular cell types. Due to the ubiquitousness of these transporters, their differential expression is involved in various disease states such as diabetes, ischemia and cancer. The majority of cancers and isolated cancer cell lines over-express the GLUT family members which are present in the respective tissue of...
Tipo: Journal article
Palavras-chave: GLUT; Glucose transporters; Expression; Cancer; Estrogen; Progesterone; Cell lines.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100004

Osteogenic molecules for clinical applications: improving the BMP-collagen system
Provedor de dados: 48
Autores: Arrabal,Pilar M; Visser,Rick; Santos-Ruiz,Leonor; Becerra,José; Cifuentes,Manuel.
Among the osteogenic growth factors used for bone tissue engineering, bone morphogenetic proteins (BMPs) are the most extensively studied for use in orthopaedic surgery. BMP-2 and BMP-7 have been widely investigated for developing therapeutic strategies and are the only two approved for use in several clinical applications. Due to the chemical and biological characteristics of these molecules, their authorised uses are always in combination with a carrier based on collagen type I. Although the use of these growth factors is considered safe in the short term, the very high doses needed to obtain significant osteoinduction make these treatments expensive and their long-term safety uncertain, since they are highly pleiotropic and have the capacity to induce...
Tipo: Journal article
Palavras-chave: BMPs; Collagen; FGF-2; Growth factors; Osteogenesis; Recombinant proteins; RGD peptide.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400013

Programming Paradigms and Mind Metaphors: Convergence and Cross-fertilization in the Study of Cognition
Provedor de dados: 48
Autores: COSMELLI,DIEGO; SOTO-ANDRADE,JORGE; TANTER,ERIC.
This paper describes a notable convergence between biological organization and programming language abstractions. Our aim is to explore possibilities of cross-fertilization, at both conceptual and empirical levels, towards the understanding of what cognition and cognitive systems might be
Tipo: Journal article
Palavras-chave: Programming Paradigms; Biologically Inspired Computing; Cognitive Systems; Software Agents; Regional Ontologies.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500011

Intake of Beer, Wine and Spirits and Risk of Heavy Drinking and Alcoholic Cirrhosis
Provedor de dados: 48
Autores: GRØNBÆK,MORTEN; JENSEN,MAJKEN K; JOHANSEN,DITTE; SØRENSEN,THORKILD I A; BECKER,ULRIK.
Studies have suggested that wine drinkers are at lower risk of death than beer or spirit drinkers. The aim of this study is to examine whether the risk of becoming a heavy drinker or developing alcoholic cirrhosis differs among individuals who prefer different types of alcoholic beverages. In a longitudinal setting we found that both the risk of becoming a heavy or excessive drinker (above 14 and 21 drinks per week for women and above 21 and 35 drinks per week for men) and the risk of developing alcoholic cirrhosis depended on the individuals preference of wine, beer or spirits. We conclude that moderate wine drinkers appear to be at lower risk of becoming heavy and excessive drinkers and that this may add to the explanation of the reported...
Tipo: Journal article
Palavras-chave: Alcohol; Alcoholic cirrhosis; Beer; Beverage preference; Excessive drinking; Heavy drinking; Spirit; Wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200004

Histidines 13 and 14 in the A&amp;#946; sequence are targets for inhibition of Alzheimer's disease A&amp;#946; ion channel and cytotoxicity
Provedor de dados: 48
Autores: DÍAZ,JUAN CARLOS; LINNEHAN,JOHN; POLLARD,HARVEY; ARISPE,NELSON.
The fact that Alzheimer's beta amyloid (A&amp;#946;) peptides forms cation channels in lipid bilayers was discovered during the course of our experiments in the laboratory of "Guayo" Rojas at NIH in Bethesda, Maryland (USA). Recently, we found that the A&amp;#946; ion channel could be blocked selectively with small peptides that copy the amino acid sequence of the predicted mouth region of the A&amp;#946; channel pore. We now have searched for the essential amino acid residues required for this blocking effect by mutations. We found that the ability of peptides to block A&amp;#946; channel activity could be lost by replacement of histidines 13 and 14 by alanine or lysine. The amino acid substitution also resulted in the loss of the capacity of the peptides...
Tipo: Journal article
Palavras-chave: Alzheimer's disease; Beta amyloid; Ion channel blockers; Peptides; Histidine; Toxicity.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300007

A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line
Provedor de dados: 48
Autores: ARANDA,EVELYN; OWEN,GARETH I.
Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have...
Tipo: Journal article
Palavras-chave: Angiogenesis; EA.hy926; HUVEC; Endothelial; Formula; In vitro assay; Matrigel.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300012

Iron and glutathione at the crossroad of redox metabolism in neurons
Provedor de dados: 48
Autores: ARACENA,PAULA; AGUIRRE,PABLA; MUÑOZ,PABLO; NÚÑEZ,MARCO T.
Neurons, as non-dividing cells, encounter a myriad of stressful conditions throughout their lifespan. In particular, there is increasing evidence that iron progressively accumulates in the brain with age and that iron-induced oxidative stress is the cause of several forms of neurodegeneration. Here, we review recent evidence that gives support to the following notions: 1) neuronal iron accumulation leads to oxidative stress and cell death; 2) neuronal survival to iron accumulation associates with decreased expression of the iron import transporter DMT1 and increased expression of the efflux transporter IREG1; and 3) the adaptive process of neurons towards iron-induced oxidative stress includes a marked increase in both the expression of the catalytic...
Tipo: Journal article
Palavras-chave: Neurons; Iron metabolism; Iron accumulation; Oxidative stress; Glutathione metabolism; Neurodegeneration.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100017

The threat from creationism to the rational teaching of biology
Provedor de dados: 48
Autores: CORNISH-BOWDEN,ATHEL; CÁRDENAS,MARÍA LUZ.
Most biologists outside the USA and a few other countries, like Australia and Canada, are under the impression that the threat to the teaching of biology represented by creationism does not concern them directly. This is unfortunately no longer true: the recent growth of creationism, especially in its pseudo-scientific manifestation known as "intelligent design", has been obvious in several countries of Western Europe, especially the UK, Germany and Poland, and it is beginning to be noticeable in Brazil, and maybe elsewhere in Latin America. The problem is complicated by the fact that there are not just two possibilities, evolution and creationism, because creationism comes in various incompatible varieties. Turkey is now a major source of creationist...
Tipo: Journal article
Palavras-chave: Education; Creationism; Intelligent design; Fundamentalism; Evolution; Natural selection.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200002

Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis
Provedor de dados: 48
Autores: WILHELM,VIVIAN; HUARACÁN,BERNARDO; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D.T.
The genes encoding the heat shock proteins HSP10 and HSP16 of the salmon pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10 coding sequence is located in an open reading frame of 291 base pairs encoding 96 aminoacids. The HSP16 coding region was isolated as a 471 base pair fragment encoding a protein of 156 aminoacids. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from other prokaryotic organisms. Both proteins were expressed in E. coli as fusion proteins with thioredoxin and purified by chromatography on Ni-column. A rabbit serum against P. salmonis total proteins reacts with the recombinant HSP10 and HSP16 proteins. Similar reactivity was determined by ELISA using serum...
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300013

A high-fat diet decreases GABA concentration in the frontal cortex and hippocampus of rats
Provedor de dados: 48
Autores: Sandoval-Salazar,Cuauhtemoc; Ramírez-Emiliano,Joel; Trejo-Bahena,Aurora; Oviedo-Solís,Cecilia I.; Solís-Ortiz,Martha Silvia.
BACKGROUND: It has been proposed that the &amp;#947;-aminobutyric acid (GABA) plays a key role in the regulation of food intake and body weight by controlling the excitability, plasticity and the synchronization of neuronal activity in the frontal cortex (FC). It has been also proposed that the high-fat diet (HFD) could disturb the metabolism of glutamate and consequently the GABA levels, but the mechanism is not yet clearly understood. Therefore, the aim of this study was to investigate the effect of a HFD on the GABA levels in the FC and hippocampus of rats RESULTS: The HFD significantly increased weight gain and blood glucose levels, whereas decreased the GABA levels in the FC and hippocampus compared with standard diet-fed rats CONCLUSIONS: HFD...
Tipo: Journal article
Palavras-chave: Obesity; GABA; Frontal cortex; Hippocampus; High-fat diet.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100015

Microtubule proteins and their post-translational forms in the cerebrospinal fluid of patients with paraparesis associated with HTLV-I infection and in SH-SY5Y cells: An in vitro model of HTLV-I-induced disease
Provedor de dados: 48
Autores: MALDONADO,HORACIO; ORTIZ-RIAÑO,EMILIO; KRAUSE,BERNARDO; BARRIGA,ANDRÉS; MEDINA,FERNANDO; PANDO,M ELSA; ALBERTI,CAROLINA; KETTLUN,ANA M; COLLADOS,LUCÍA; GARCÍA,LORENA; CARTIER,LUIS; VALENZUELA,M ANTONIETA.
HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is characterized by axonal degeneration of the corticospinal tracts. The specific requirements for transport of proteins and organelles to the distal part of the long axon are crucial in the corticospinal tracts. Microtubule dysfunction could be involved in this disease, configuring an axonal transport disease. We measured tubulin and its post-translational modified forms (acetylated and tyrosinated) in CSF of patients and controls, as well as tau and its phosphorylated forms. There were no significant differences in the contents of tubulin and acetyl-tubulin between patients and controls; tyrosyl-tubulin was not detected. In HAM/TSP, tau levéis were significantly reduced, while the ratio...
Tipo: Journal article
Palavras-chave: Tropical spastic paraparesis; Cerebrospinal fluid; SH-SY5Y cells; Retraction; Tau; Phosphorylated-tau forms; Tubulin; Acetyl-tubulin.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300001

Premio de Ciencias Exactas y Naturales y de Tecnología 2000,
Provedor de dados: 48
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100008

Alcohol, Wine and Platelet Function
Provedor de dados: 48
Autores: RUF,JEAN-CLAUDE.
Epidemiological studies have demonstrated an inverse correlation between moderate wine and alcohol consumption and morbidity and mortality from coronary heart disease. The protective effect has been associated with an increase in the plasma level of HDL cholesterol, as it is well recognized that plasma HDL is inversely correlated with CHD. In addition, it has become evident that blood platelets contribute to the rate of development of atherosclerosis and CHD through several mechanisms. In recent studies it has been shown that the level of HDL cholesterol can explain only 50 % of the protective effect of alcoholic beverages; the other 50 % may be partly related to a decrease in platelet activity. This anti-platelet activity of wine is explained by ethanol...
Tipo: Journal article
Palavras-chave: Alcohol; Cardiovascular disease; Platelet function; Wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200006

Cytohesins and centaurins control subcellular trafficking of macromolecular signaling complexes: Regulation by phosphoinositides and ADP-Ribosylation Factors
Provedor de dados: 48
Autores: HAWADLE,MUHAMED A.; FOLARIN,NAJEEM; MARTIN,ROSEMARY; JACKSON,TREVOR R.
The ADP-ribosylation factor family of small GTP-binding proteins are implicated in the regulation of vesicular transport and control of cytoskeletal and cell adhesion events. The phosphoinositide 3-kinase, phosphoinositide 4-P 5-kinase and phospholipase D signaling pathways are major regulators of ARF signaling cascades. Two families of ARF regulatory molecules, the cytohesin ARF-Guanine nucleotide Exchange Factors and the centaurin GTPase-Activating Proteins provide key targets for the action of these lipid signals. A critical feature of the regulation of ARF signaling is coordinated recruitment of exchange factors, ARFs and GAPs to appropriate subcellular locations. These complexes drive repetitive cycles of ARF activation and membrane association that...
Tipo: Journal article
Palavras-chave: ADP-ribosylation factor Cytohesin; Centaurin GTPase Activating Protein Guanine nucleotide Exchange Factor Phosphoinositide.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200017

Fortalecimiento de las ciencias biológicas en Chile durante la era posgenómica
Provedor de dados: 48
Autores: Jerez,Carlos A.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100001

Heterogeneous periodicity of drosophila mtDNA: new refutations of neutral and nearly neutral evolution
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
We found a consistent 3-site periodicity of the X²9 values for the heterogeneity of the distribution of the second base in relation to the first base of dinucleotides separated by 0 (contiguous), 1, 2, 3 ... 17 (K) nucleotide sites in Drosophila mtDNA. Triplets of X²9 values were found where the first was over 300 and the second and third ranged between 37 and 114 (previous studies). In this study, the periodicity was significant until separation of 2011K, and a structure of deviations from randomness among dinucleotides was found. The most deviant dinucleotides were G-G, G-C and C-G for the first, second and third element of the triplet, respectively. In these three cases there were more dinucleotides observed than expected. This inter-bases correlation...
Tipo: Journal article
Palavras-chave: DNA organization; Non-protein-coding evolution; Ordered nucleotide sequences; Inter-base associations; Refutation of neutralism.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300010

Mejorando la gestión editorial
Provedor de dados: 48
Tipo: Journal article
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100001

Hepatocytes, rather than leukocytes reverse DNA damage in vivo induced by whole body y-irradiation of mice, as shown by the alkaline comet assay
Provedor de dados: 48
Autores: PINCHEIRA,JUANA; CARRERA,PILAR; MARCELAIN,KATHERINE; DE LA TORRE,CONSUELO.
DNA damage repair was assessed in quiescent (G0) leukocytes and in hepatocytes of mice, after 1 and 2 hours recovery from a single whole body y-irradiation with 0.5, 1 or 2 Gy. Evaluation of single-strand breaks (SSB) and alkali-labile sites together were carried out by a single-cell electrophoresis at pH&gt;13.0 (alkaline comet assay). In non-irradiated (control) mice, the constitutive, endogenous DNA damage (basal) was around 1.5 times higher in leukocytes than in hepatocytes. Irradiation immediately increased SSB frequency in both cell types, in a dose-dependent manner. Two sequential phases took place during the in vivo repair of the radio-induced DNA lesions. The earliest one, present in both hepatocytes and leukocytes, further increased the SSB...
Tipo: Journal article
Palavras-chave: Alkaline comet assay; Ionizing radiation; Hepatocytes; Leukocytes; In vivo DNA repair.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200011

Seed germination of Agave species as influenced by substrate water potential
Provedor de dados: 48
Autores: Ramírez-Tobías,Hugo M; Peña-Valdivia,Cecilia B; Trejo,Carlos; R,J Rogelio Aguirre; Humberto Vaquera,H.
BACKGROUND: Plants of Agave spp. perform Crassulacean acid metabolism (CAM) and are highly drought-tolerant, but little is known concerning seed germination under low water availability. The aim of this study was to assess the effect of substrate water potential (&amp;#936;W) on seed germination and contrast hydrotime parameters of seven valuable and commercially-important Agave species from different geographical distributions and climatic regions of Mexico. Our hypothesis was that seed germination of Agave species is not affected by low water availability independently of seed biomass and the climate of their distribution area. RESULTS: Seed germination (at 25°C and in the dark) between 85 and 100% for all species occurred within 80 - 180 h at -0.03 MPa...
Tipo: Journal article
Palavras-chave: Base water potential; CAM; Drought; Germination; Hydrotime model; Maguey.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100011

The TLR7 agonist Imiquimod promote the immunogenicity of msenchymal stem cells
Provedor de dados: 48
Autores: Zhang,Li; Liu,Dan; Pu,Dan; Wang,Yanwen; Li,Li; He,Yanqi; Li,Yalun; Li,Lei; Li,Weimin.
BACKGROUND: Mesenchymal stem cells (MSCs) are considered the best candidate in stem cells therapy due to their multipotent differentiation ability, low expression of co-stimulatory molecules (CD80, CD86, CD34 and HLA-II) and immunosuppression effects on in vivo immune responses. MSCs were now widely used in clinical trials but received no encourage results. The major problem was the fate of engrafted MSCs in vivo could not be defined. Some studies indicated that MSCs could induce immune response and result in the damage and rejection of MSCs. As toll like receptors (TLRs) are important in inducing of immune responses, in this study we study the role of TLR7 in mediating the immune status of MSCs isolated from umbilical cord. RESULTS: Our results indicated...
Tipo: Journal article
Palavras-chave: Mesenchymal stem cells; Umbilical cord; Toll like receptor 7; Immunogenicity; Pro-inflammatory molecules.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100006

Cobalt oxide nanoparticles aggravate DNA damage and cell death in eggplant via mitochondrial swelling and NO signaling pathway
Provedor de dados: 48
Autores: Faisal,Mohammad; Saquib,Quaiser; Alatar,Abdulrahman A.; Al-Khedhairy,Abdulaziz A.; Ahmed,Mukhtar; Ansari,Sabiha M.; Alwathnani,Hend A.; Dwivedi,Sourabh; Musarrat,Javed; Praveen,Shelly.
BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron...
Tipo: Journal article
Palavras-chave: Cobalt oxide nanoparticles; Nanotoxicity; DNA damage; Apoptosis; Oxidative stress.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100020

The subsidiary GntII system for gluconate metabolism in Escherichia coli: Alternative induction of the gntV gene
Provedor de dados: 48
Autores: Gómez,Keyla M; Rodríguez,Andrea; Rodriguez,Yesseima; Ramírez,Alvaro H; Istúriz,Tomás.
Two systems are involved in the transport and phosphorylation of gluconate in Escherichia coli. GntI, the main system, consists of high and low-affinity gluconate transporters and a thermoresistant gluconokinase for its phosphorylation. The corresponding genes, gntT, gntU and gntK at 76.5 min, are induced by gluconate. GntII, the subsidiary system, includes IdnT and GntV, which duplicate activities of transport and phosphorylation of gluconate, respectively. Gene gntV at 96.8 min is divergently transcribed from the idnDOTR operon involved in L-idonate metabolism. These genetic elements are induced by the substrate or 5-keto-D-gluconate. Because gntV is also induced in cells grown in gluconate, it was of interest to investigate its expression in this...
Tipo: Journal article
Palavras-chave: E. coli; Gluconate; GntII; GntV.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300008

Symmorphosis in the proximal pathway for oxygen in the leaf-eared mouse Phyllotis darwini
Provedor de dados: 48
Autores: CANALS,MAURICIO; FIGUEROA,DANIELA; SABAT,PABLO.
In this report, we explore the matching of structures to functional needs by comparing previously reported data of maximal oxygen consumption and the development of the lung in the leaf-eared mouse Phyllotis darwini in warm and cold environments. We discuss whether the state of structural design is commensurate with functional needs from regulated morphogenesis as predicted by the hypothesis of symmorphosis. We found a close match between respiratory structures and functional needs during postnatal development, expressed as safety factors close to unity. However, in the adult stage the safety factors were greater than two, which suggests that adult animals acquired a structure greater than that required considering their maximum capacities. A high safety...
Tipo: Journal article
Palavras-chave: Metabolism; Ontogeny; Phyllotis darwini; Symmorphosis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100009

Suppression of miR-181 a attenuates H2O2-induced death of mesenchymal stem cells by maintaining hexokinase II expression
Provedor de dados: 48
Autores: Lee,Seahyoung; Yun,Ina; Ham,Onju; Lee,Se-Yeon; Lee,Chang Yeon; Park,Jun-Hee; Lee,Jiyun; Seo,Hyang-Hee; Choi,Eunhyun; Hwang,Ki-Chul.
BACKGROUND: Low survival rate of transplanted cells compromises the efficacy of cell therapy. Hexokinase II (HKII) is known to have anti-apoptotic activity through its interaction with mitochondria. The objective was to identify miRNAs targeting HKII and investigate whether miRNA-mediated modulation of HKII could improve the survival of mesenchymal stem cells (MSCs) exposed to H2O2. The expression of HKII in MSCs exposed to H2O2 was evaluated, and HKII-targeting miRNA was screened based on miRNA-target prediction databases. The effect of H2O2 on the expression of the selected HKII-targeting miRNA was examined and the effect of modulation of the selected HKII-targeting miRNA using anti-miRNA on H2O2-induced apoptosis of MSC was evaluated. RESULTS: H2O2 (600...
Tipo: Journal article
Palavras-chave: Hexokinase II; MiRNA-181a; Cell death; Mesenchymal stem cell.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100045

Investigations of anticholinestrase and antioxidant potentials of methanolic extract, subsequent fractions, crude saponins and flavonoids isolated from Isodon rugosus
Provedor de dados: 48
Autores: Zeb,Anwar; Sadiq,Abdul; Ullah,Farhat; Ahmad,Sajjad; Ayaz,Muhammad.
BACKGROUND: Based on the ethnomedicinal uses and the effective outcomes of natural products in various diseases, this study was designed to evaluate Isodon rugosus as possible remedy in oxidative stress, alzheimer's and other neurodegenerative diseases. Acetylecholinestrase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of crude methanolic extract (Ir.Cr), resultant fractions (n-hexane (Ir.Hex), chloroform (Ir.Cf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq)), flavonoids (Ir.Flv) and crude saponins (Ir.Sp) of I. rugosus were investigated using Ellman's spectrophotometric method. Antioxidant potential of I. rugosus was determined using DPPH, H2O2 and ABTS free radicals scavenging assays. Total phenolic and flavonoids contents of plant extracts...
Tipo: Journal article
Palavras-chave: Anticholinesterase; Antioxidant; Flavonoids; Phenolics; Saponins; Isodon rugosus.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100070

Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
Provedor de dados: 48
Autores: MAINARDES,RUBIANA M; PALMIRA D. GREMIÃO,MARIA.
The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91:9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL"¹ (r² &gt; 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of...
Tipo: Journal article
Palavras-chave: RP-HPLC; Validation; AZT; Pharmacokinetics; Intranasal delivery.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300010

Lipid rafts: cell surface platforms for T cell signaling
Provedor de dados: 48
Autores: MAGEE,TONY; PIRINEN,NIINA; ADLER,JEREMY; PAGAKIS,STAMATIS N; PARMRYD,INGELA.
The Src family tyrosine kinase Lck is essential for T cell development and T cell receptor (TCR)* signaling. Lck is post-translationally fatty acylated at its N-terminus conferring membrane targeting and concentration in plasma membrane lipid rafts, which are lipid-based organisational platforms. Confocal fluorescence microscopy shows that Lck colocalises in rafts with GPI-linked proteins, the adaptor protein LAT and Ras, but not with non-raft membrane proteins including the protein tyrosine phosphatase CD45. The TCR also associates with lipid rafts and its cross-linking causes coaggregation of raft-associated proteins including Lck, but not of CD45. Cross-linking of either the TCR or rafts strongly induces specific tyrosine phosphorylation of the TCR in...
Tipo: Journal article
Palavras-chave: Lipid rafts; Lck; Tyrosine kinase; T cell; Signalling; Phosphatase.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200003

Introduction to supramolecular complex formation in cell signaling and disease
Provedor de dados: 48
Autores: LEYTON,LISETTE; QUEST,ANDREW F. G.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200002

Inhibition of iron and copper uptake by iron, copper and zinc
Provedor de dados: 48
Autores: ARREDONDO,MIGUEL; MARTÍNEZ,RONNY; NÚÑEZ,MARCO T; RUZ,MANUEL; OLIVARES,MANUEL.
Interactions of micronutrients can affect absorption and bioavailability of other nutrients by a number of mechanisms. In aqueous solutions, and at higher uptake levels, competition between elements with similar chemical characteristics and uptake process can take place. The consequences of these interactions may depend on the relative concentrations of the nutrients. In this work, we measure the effects of increasing concentrations of iron, zinc, and copper on iron and copper uptake in Caco-2 cells. Intracellular Fe or Cu levels were affected by incubating with increased concentrations of metals. However, when the cells already had different intracellular metal concentration, the uptake of Fe or Cu was nor affected. In competition studies, we showed that...
Tipo: Journal article
Palavras-chave: Competition; Interaction; Metals; Uptake.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100011

Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
Provedor de dados: 48
Autores: CHARRIER,MAR YVONNE; FONTY,GERARD; GAILLARD-MARTINIE,BRIGITTE; AINOUCHE,KADER; ANDANT,GERARD.
The intestinal microbiota of the edible snails Cornu aspersum fSyn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considered. The cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) CFU g -1, but the Swedish H. pomatia appeared significantly less colonised, suggesting a higher sensitivity of its microbiota to climatic change. All the strains, but one, shared &amp;#8805; 97% sequence identity with reference strains. They were arranged into two taxa: the Gamma Proteobacteria with Buttiauxella, Citrobacter, Enterobacter, Kluyvera, Obesumbacterium,...
Tipo: Journal article
Palavras-chave: Clostridium; Edible snails; Enterobacteriaceae; Enterococci; Gut microbiota; Lactococci.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500010

The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
Provedor de dados: 48
Autores: ZIMMERMANN,KATRIN; HOLTZ,MARLIS; PHI-VAN,LOC.
We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase
Tipo: Journal article
Palavras-chave: Chromatin structure; Origin of bidirectional replication; Lysozyme; GAS41; Promoter.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200009

Genetic polymorphism of clinical and environmental strains of Pichia anomala
Provedor de dados: 48
Autores: REYES,EUGENIO; BARAHONA,SALVADOR; FISCHMAN,OLGA; NIKLITSCHEK,MAURICIO; BAEZA,MARCELO; CIFUENTES,VÍCTOR.
In this work 20 clinical and 3 environmental yeast isolates were characterized by classical morphological and physiological methods, as well as molecular methods based on PCR of the ITS1-5.8S rDNA-ITS2 region. The characteristic morphology and biochemical profiles observed in these samples correspond to those described for the Pichia genera, more specifically to P. anomala. The profiles of susceptibility to five antifungal drugs were determined by two broth dilution methods. The results obtained by both methods were comparable and showed that clinical isolates presented more resistance to azoles, amphotericin B, and 5-fluorocytosine, than environmental ones did. By amplification and sequencing of internal transcribed spacers (ITS1 and ITS2) and the...
Tipo: Journal article
Palavras-chave: Pichia anomala; 5.8S rDNA; RAPD; Internal transcribed spacer; Antifungal drugs.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500004

Cancer stem cells - the current status of an old concept: literature review and clinical approaches
Provedor de dados: 48
Autores: Fulawka,Lukasz; Donizy,Piotr; Halon,Agnieszka.
As regards their morphology and biology, tumours consist of heterogeneous cell populations. The cancer stem cell (CSC) hypothesis assumes that a tumour is hierarchically organized and not all of the cells are equally capable of generating descendants, similarly to normal tissue. The only cells being able to self-renew and produce a heterogeneous tumour cell population are cancer stem cells. CSCs probably derive from normal stem cells, although progenitor cells may be taken into consideration as the source of cancer stem cells. CSCs reside in the niche defined as the microenvironment formed by stromal cells, vasculature and extracellular matrix. The CSC assays include FACS sorting, xenotransplantation to immunodeficient mice (SCID), incubation with Hoechst...
Tipo: Journal article
Palavras-chave: Cancer stem cells; Stem cells; Tumour-initiating cells; Tumour-propagating cells; Carcinogenesis; Tumour heterogeneity; Clonal evolution.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100060

Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats
Provedor de dados: 48
Autores: Vargas Guerrero,Belinda; García López,Pedro M; González Santiago,Ana E; Domínguez Rosales,José A; Gurrola Díaz,Carmen M.
Objective: The high global incidence of type 2 diabetes has challenged researchers to establish animal models that resemble the chronic stage observed in type 2 diabetes patients. One such model is induced by neonatal streptozotocin (n-STZ) administration to rat pups at 0, 2, or 5 days after birth. In this study, we assessed lns-1 gene expression and tissue insulin levels as well as serum concentration of glucose and insulin, insulin resistance, and histological changes of the islets of Langerhans in n5-STZ rats after 20-weeks post-induction. Methods: Wistar rat pups were randomly distributed into a control group and a streptozotocin-induced group. Experimental induction involved a single intraperitoneal injection of streptozotocin (150 mg/kg) into...
Tipo: Journal article
Palavras-chave: Chronic hyperglycemia; In vivo diabetes models; Lns-1 gene; Type 2 diabetes.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300009

Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
Provedor de dados: 48
Autores: Shanshiashvili,Lali; Tsitsilashvili,Elene; Dabrundashvili,Nino; Kalandadze,Irine; Mikeladze,David.
Abstract  Background  Macrophages are a functionally heterogeneous cell population and depending on microenvironments they polarize in two main groups: M1 and M2. Glutamic acid and glutamate receptors may participate in the regulation of macrophage plasticity. To investigate the role of glutamatergic systems in macrophages physiology, we performed the transfection of mGluR5 cDNAs into RAW-264.7 cells.  Results  Comparative analysis of modified (RAW-mGluR5 macrophages) and non-modified macrophages (RAW-macrophages) has shown that the RAW-mGluR5 macrophages absorbed more glutamate than control cells and the amount of intracellular glutamate correlated with the expression of excitatory amino acid transporters -2 (EAAT-2). Besides, our results have shown that...
Tipo: Journal article
Palavras-chave: Macrophages; Metabotropic glutamate receptors; Inflammation; HMGB1; IL-10.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100203

Biological evaluation of novel 6-Arylbenzimidazo [1,2-c] quinazoline derivatives as inhibitors of LPS-induced TNF- alpha secretion
Provedor de dados: 48
Autores: GALARCE,GLORIA D; FONCEA,ROCÍO E; EDWARDS,ANA M; PESSOA-MAHANA,HERNÁN; PESSOA-MAHANA,CARLOS D; EBENSPERGER,ROBERTO A.
This study describes the effect of novel 6-Arylbenzimidazo [1,2-c] quinazoline derivatives as tumor necrosis factor alpha (TNF-á) production inhibitors. The newly synthesized compounds were tested for their in vitro ability to inhibit the lipolysaccharide (LPS) induced TNF-á secretion in the human promyelocytic cell line HL-60. The compound 6-Phenyl-benzimidazo [1,2-c] quinazoline, coded as Gl, resulted as the most potent inhibitor and with no significant cytotoxic activity. Thus, 6-Arylbenzimidazo [1,2-c] quinazoline derivatives may have a potential as anti-inflammatory agents
Tipo: Journal article
Palavras-chave: Anti-inflammatory agents; Benzimidazoquinazoline derivatives; TNF-á inhibitors.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100006

Genomic organization of the structural genes controlling the astaxanthin biosynthesis pathway of Xanthophyllomyces dendrorhous
Provedor de dados: 48
Autores: NIKLITSCHEK,MAURICIO; ALCAÍNO,JENNIFER; BARAHONA,SALVADOR; SEPÚLVEDA,DIONISIA; LOZANO,CARLA; CARMONA,MARISELA; MARCOLETA,ANDRÉS; MARTÍNEZ,CLAUDIO; LODATO,PATRICIA; BAEZA,MARCELO; CIFUENTES,VÍCTOR.
The cloning and nucleotide sequence of the genes (idi, crtE, crtYB, crtl and crtS) controlling the astaxanthin biosynthesis pathway of the wild-type ATCC 24230 strain of Xanthophyllomyces dendrorhous in their genomic and cDNA version were obtained. The idi, crtE, crtYB, crtl and crtS genes were cloned, as fragments of 10.9, 11.5, 15.8, 5.9 and 4 kb respectively. The nucleotide sequence data analysis indicates that the idi, crtE, crtYB, crtl and crtS genes have 4, 8,4, 11, and 17 introns and 5, 9, 5, 12 and 18 exons respectively. In addition, a highly efficient site-directed mutagenesis system was developed by transformation by integration, followed by mitotic recombination (the double recombinant method). Heterozygote idi (idi+ / idi-::hph), crtE (crtE+ /...
Tipo: Journal article
Palavras-chave: Astaxanthin biosynthesis; Xanthophyllomyces dendrorhous; Phaffia rhodozyma.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100011

Cytoprotective and suicidal signaling in oxidative stress
Provedor de dados: 48
Autores: Videla,Luis A.
Oxidative stress is an imbalance between pro-oxidants and antioxidants in favor of the pro-oxidants, leading to different responses depending on the level of pro-oxidants achieved and the duration of exposure. In this article, we discuss the cytoprotective or suicidal signaling mechanisms associated with oxidative stress by addressing: (i) the development of an acute and mild pro-oxidant state by thyroid hormone administration eliciting the redox upregulation of the expression of proteins affording cell protection as a preconditioning strategy against ischemia-reperfusion liver injury; and (ii) the role of prolonged and severe oxidative stress and insulin resistance as determinant factors in the pathogenesis of non-alcoholic fatty liver disease associated...
Tipo: Journal article
Palavras-chave: Oxidative stress; Thyroid hormone; Liver preconditioning; Obesity; Insulin resistance; Non-alcoholic fatty liver disease.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300014

Morphodynamical enaction: the case of color
Provedor de dados: 48
Autores: PETITOT,JEAN.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100009

New invariants and dimensionless numbers: futile renaissance of old fallacies?
Provedor de dados: 48
Autores: NESPOLO,ROBERTO F.
The necessity of the mathematical description of living processes and the recent advances in theoretical syntheses that fit surprisingly well with real data have led many scientists to experiment with new "generalizations," beginning with allometric equations. I present here, briefly, why caution is needed when allometric equations are multiplied or divided to make new ones. This practice is flawed by old and recognized problems, such as the fallacy of averages combined with misunderstandings that include error propagation, misuse of statistics and confounding scale.
Tipo: Journal article
Palavras-chave: Fallacy of averages; Dimensional analysis; Invariants; Allometric equations; Error term.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100004

Novel identification and characterisation of Transient receptor potential melastatin 3 ion channels on Natural Killer cells and B lymphocytes: effects on cell signalling in Chronic fatigue syndrome/Myalgic encephalomyelitis patients
Provedor de dados: 48
Autores: Nguyen,T.; Staines,D.; Nilius,B.; Smith,P.; Marshall-Gradisnik,S.
BACKGROUND: Transient receptor potential melastatin 3 (TRPM3) cation channels are ubiquitously expressed by multiple cells and have an important regulatory role in calcium-dependent cell signalling to help maintain cellular homeostasis. TRPM3 protein expression has yet to be determined on Natural Killer (NK) cells and B lymphocytes. Multiple single nucleotide polymorphisms have been reported in TRPM3 genes from isolated peripheral blood mononuclear cells, NK and B cells in Chronic fatigue syndrome/Myalgic encephalomyelitis (CFS/ME) patients and have been proposed to correlate with illness presentation. The object of the study was to assess TRPM3 surface expression on NK and B lymphocytes from healthy controls, followed by a comparative investigation...
Tipo: Journal article
Palavras-chave: Chronic fatigue syndrome; Transient receptor potential; Calcium signalling; Myalgic encephalomyelitis.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100027

Código de Ética de la Sociedad de Biología de Chile
Provedor de dados: 48
Tipo: Journal article
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100002

Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
Provedor de dados: 48
Autores: FIUZA,TATIANA S; SILVA,PAULO C; PAULA,JOSÉ R DE; TRESVENZOL,LEONICE M F; SABÓIA-MORAIS,SIMONE M T.
This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an experimental model. The ethanol extract and fractions were administered to the fish orally with their feed. Twenty-four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that the crude extract and the ethyl,...
Tipo: Journal article
Palavras-chave: Fish; Medicinal plants; Vasodilation.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400002

Complete sequence of the genome of the human isolate of Andes virus CHI-7913: comparative sequence and protein structure analysis
Provedor de dados: 48
Autores: TISCHLER,NICOLE D; FERNÁNDEZ,JORGE; MÜLLER,ILSE; MARTÍNEZ,RODRIGO; GALENO,HÉCTOR; VILLAGRA,ELIECER; MORA,JUDITH; RAMÍREZ,EUGENIO; ROSEMBLATT,MARIO; VALENZUELA,PABLO D.T.
We report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M, and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein...
Tipo: Journal article
Palavras-chave: Hantavirus; Genome sequence; RNA polymerase; Human isolate.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200010

Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.
Provedor de dados: 48
Autores: NK,Asha Tukappa; Londonkar,Ramesh L; Nayaka,Hanumantappa B; CB,Sanjeev Kumar.
BACKGROUND: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 &amp;#956;g/ml. RESULTS: Pretreatment of animals with whole plant...
Tipo: Journal article
Palavras-chave: Rumex vesicarius L; HepG2; MDA; SOD; CAT; CCl4 induced hepatotocixity.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100019

Social stimuli cause changes of plasma oxytocin and behavior in guinea pigs
Provedor de dados: 48
Autores: WALLNER,BERNARD; DITTAMI,JOHN; MACHATSCHKE,IVO.
The neuropeptide oxytocin (OXT) is a key factor in the initiation and regulation of sociosexual behavior. The present study analyzes the effects of cohabitation and social challenge on plasma OXT concentration rates in guinea pig pairs in relation to male sociosexual behavior. The cohabitation phase lasted 3 days. On day 4, the pair was socially challenged by introducing an unfamiliar male. Displayed male sexual behavior varied significantly during cohabitation, with peaks on day 1. Sociopositive behavior, i.e., side-by-side contact, was increased on days 3 and 4. Cohabitation per se led to elevated plasma OXT concentrations only in males. In contrast, both sexes reacted with increased plasma OXT concentrations to the social challenge (day 4). At that...
Tipo: Journal article
Palavras-chave: Oxytocin; Behavior guinea pig social challenge cohabitation.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200007

Acute oral toxicity and anti-inflammatory activity of hydroalcoholic extract from Lampaya medicinalis Phil in rats
Provedor de dados: 48
Autores: Morales,Glauco; Paredes,Adrián; Olivares,Alberto; Bravo,Jaime.
BACKGROUND: Algesia and inflammation are related with several pathological conditions. It is known that many drugs available for the treatment of these problems cause unwanted side effects. This study was aimed at evaluating acute toxicity and anti-inflammatory activity of Lampaya medicinalis Phil. (Verbenaceae) widely used in the folk medicine of Northern Chile against rheumatism, arthritis and body joints pain. RESULTS: Oral administration of hydroalcoholic extract (HAE) at the highest dose of 3000 mg/ Kg body weight resulted in no mortalities or evidence of significant behavioral changes. Histological examination revealed normal architecture and no significant adverse effects were observed on the liver, kidney, heart, lung or ovaries and testicles. The...
Tipo: Journal article
Palavras-chave: Lampaya medicinalis; Hydroalcoholic extract; Acute oral toxicity; Histology; Hematology; Anti-inflammatory; Carrageenan.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100006

Mechanical ventilation and volutrauma: study in vivo of a healthy pig model
Provedor de dados: 48
Autores: Pastore,Camilla V; Pirrone,Federica; Mazzola,Silvia; Rizzi,Manuela; Viola,Manuela; Sironi,Giuseppe; Albertini,Mariangela.
Mechanical ventilation is essential in intensive care units. However, it may itself induce lung injury. Current studies are based on rodents, using exceptionally large tidal volumes for very short periods, often after a "priming" pulmonary insult. Our study deepens a clinically relevant large animal model, closely resembling human physiology and the ventilator setting used in clinic settings. Our aim was to evaluate the pathophysiological mechanisms involved in alveolo/capillary barrier damage due to mechanical stress in healthy subjects. We randomly divided 18 pigs (sedated with medetomidine/tiletamine-zolazepam and anesthetised with thiopental sodium) into three groups (n=6): two were mechanically ventilated (tidal volume of 8 or 20 ml/kg), the third...
Tipo: Journal article
Palavras-chave: Gelatinases; Mechanical ventilation; Pig; Ventilator induced lung injury (VILI).
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300002

An "enactive" approach to integrative and comparative biology: Thoughts on the table
Provedor de dados: 48
Autores: PALACIOS,ADRIAN G.; BOZINOVIC,FRANCISCO.
We discuss the concept of Enaction as originally proposed by Varela. We attempt to exemplify through two specific topics, sensory ecology and behavior, as well as physiological and behavioral ecology, on which the enactive approach is based. We argue that sensory physiology allows us to explore the biological and cognitive meaning of animal 'private' sensory channels, beyond the scope of our own sensory capacity. Furthermore, after analyzing the interplay between factors that may impose limits upon an animal's use of time and energy, we call for a program of research in integrative and comparative biology that simultaneously considers evolutionary ecology (including physiological and behavioral ecology) and neurobiology (including cognitive mechanisms as...
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100008

Roles of nibrin and ATM/ATR kinases on the G2 checkpoint under endogenous or radio-induced DNA damage
Provedor de dados: 48
Autores: Marcelain,Katherine; de la Torre,Consuelo; González,Patricio; Pincheira,Juana.
Checkpoint response to DNA damage involves the activation of DNA repair and G2 lengthening subpathways. The roles of nibrin (NBS1) and the ATM/ATR kinases in the G2 DNA damage checkpoint, evoked by endogenous and radio-induced DNA damage, were analyzed in control, A-T and NBS lymphoblast cell lines. Short-term responses to G2 treatments were evaluated by recording changes in the yield of chromosomal aberrations in the ensuing mitosis, due to G2 checkpoint adaptation, and also in the duration of G2 itself. The role of ATM/ATR in the G2 checkpoint pathway repairing chromosomal aberrations was unveiled by caffeine inhibition of both kinases in G2. In the control cell lines, nibrin and ATM cooperated to provide optimum G2 repair for endogenous DNA damage. In...
Tipo: Journal article
Palavras-chave: Ataxia-telangiectasia; ATM; ATR kinases; Caffeine; G2 checkpoint; Nibrin (Nbs1); Nijmegen breakage syndrome (NBS).
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200007

Molecular analysis of the eighteen most frequent mutations in the BRCA1 gene in 63 Chilean breast cancer families
Provedor de dados: 48
Autores: JARA,LILIAN; AMPUERO,SANDRA; SANTIBÁÑEZ,EUDOCIA; SECCIA,LORENA; RODRÍGUEZ,JUAN; BUSTAMANTE,MARIO; LAY-SON,GUILLERMO; OJEDA,JOSÉ MANUEL; REYES,JOSÉ MIGUEL; BLANCO,RAFAEL.
BRCA1 gene mutations account for nearly all families with multiple cases of both early onset breast and/or ovarian cancer and about 30% of hereditary breast cancer. Although to date more than 1,237 distinct mutations, polymorphisms, and variants have been described, several mutations have been found to be recurrent in this gene. We have analyzed 63 Chilean breast/ovarian cancer families for eighteen frequent BRCA1 mutations. The analysis of the five exons and two introns in which these mutations are located was made using mismatch PCR assay, ASO hybridization assay, restriction fragment analysis, allele specific PCR assay and direct sequentiation techniques. Two BRCA1 mutations (185delAG and C61G) and one variant of unknown significance (E1250K) were found...
Tipo: Journal article
Palavras-chave: BRCA1 gene; Frequent mutations; Polymorphism; 4185delCAAG; New mutation; Chilean population.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300011

XV-2c and KM: 19 haplotype analysis in Chilean patients with cystic fibrosis and unknown CFTR gene mutations
Provedor de dados: 48
Autores: REPETTO,GABRIELA M; PUGA,ALONSO R; DELGADO,IRIS.
Cystic fibrosis (CF) is caused by mutations in the CFTR gene. More than 1600 mutations have been described, with frequencies that differ worldwide according to the ethnic origin of patients. A small group of mutations are recurrent on several populations. It has been shown that they each tend occur on specific chromosome 7 haplotypes, supporting the notion of a single origin for them. Less than 50% of mutations in Chilean patients have been identified to date. To indirectly assess the possible presence of a predominant founder mutation in the remaining unknown alíeles, we evaluated 2 polymorphic markers, XV-2c and KM.19, tightly linked to the CFTR locus. The study was done in Chilean CF patients with unknown or delt F508 ( F508) CFTR mutations and their...
Tipo: Journal article
Palavras-chave: CFTR gene; Cystic fibrosis; F508; Haplotype analys.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200013

Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
Provedor de dados: 48
Autores: SCHREIER,STEFAN; TRIAMPO,WANNAPONG; DOUNGCHAWEE,GALAYANEE; TRIAMPO,DARAPOND; CHADSUTHI,SUDARAT.
Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique...
Tipo: Journal article
Palavras-chave: Leptospirosis; Leptospira; Enumeration; Spectroscopy; Indirect counting.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001

Foundational errors in the Neutral and Nearly-Neutral theories of evolution in relation to the Synthetic Theory: Is a new evolutionary paradigm necessary?
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
The Neutral Theory of Evolution (NTE) proposes mutation and random genetic drift as the most important evolutionary factors. The most conspicuous feature of evolution is the genomic stability during paleontological eras and lack of variation among taxa; 98% or more of nucleotide sites are monomorphic within a species. NTE explains this homology by random fixation of neutral bases and negative selection (purifying selection) that does not contribute either to evolution or polymorphisms. Purifying selection is insufficient to account for this evolutionary feature and the Nearly-Neutral Theory of Evolution (N-NTE) included negative selection with coefficients as low as mutation rate. These NTE and N-NTE propositions are thermodynamically (tendency to random...
Tipo: Journal article
Palavras-chave: Fixation; Loss; Neutral evolution; Polymorphism; Randomness; Selectiveness.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200001

The PcACE1 transcription factor from Phanerochaete chrysosporium contains a Cys- and Ser-rich transactivation domain
Provedor de dados: 48
Autores: Barham,Feiruz; Roman,Patricia; Bull,Paulina.
Transcription factor Ace1 from Saccharomyces cerevisiae regulates the expression of target genes when the copper concentration reaches 200 ìÌ levels. We are studying the ortholog of Ace1 from fungus Phanerochaete chrysosporium PcACE1, isolated by complementation in yeast. In this report we show the localization of the transactivation region of PcACE1. Different PcACE1 fragments were ligated in frame to the GAL4 DNA-binding domain by site-directed mutagenesis in a suitable yeast expression vector. Transformation of an appropriate Saccharomyces cerevisiae strain was used as host. This strain contains the fusion GAL1:lacZ in its genome under the control of promoter sequences recognized by GAL4. Finally, we measured â-galactosidase activity in each yeast...
Tipo: Journal article
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400006

Cyclooxygenase-2 and hypoxia-regulated proteins are modulated by basic fibroblast growth factor in acute renal failure
Provedor de dados: 48
Autores: Villanueva,Sandra; Escobar,Pía; Jacubovsky,Ioram; Irarrázabal,Carlos; Carreño,Juan E; Erpel,José M; Céspedes,Carlos; González,Alexis A; Vio,Carlos P; Velarde,Victoria.
Acute renal failure (ARF) can be caused by injuries that induce tissue hypoxia, which in turn can trigger adaptive or inflammatory responses. We previously showed the participation of basic fibroblast growth factor (FGF-2) in renal repair. Based on this, the aim of this study was to analyze the effect of FGF-2 signaling pathway manipulation at hypoxia-induced protein levels, as well as in key proteins from the vasoactive systems of the kidney. We injected rat kidneys with FGF-2 recombinant protein (r-FGF) or FGF-2 receptor antisense oligonucleotide (FGFR2-ASO) after bilateral ischemia, and evaluated the presence of iNOS, EPO and HO-1, in representation of hypoxia-induced proteins, as well as COX-2, renin, kallikrein, and B2KR, in representation of the...
Tipo: Journal article
Palavras-chave: ARF; COX-2; FGF-2; Kidney regeneration.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100007

VI CONGRESO DE LA SOCIEDAD CHILENA DE INMUNOLOGÍA
Provedor de dados: 48
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300013

Robertsonian chromosomes and the nuclear architecture of mouse meiotic prophase spermatocytes
Provedor de dados: 48
Autores: Berríos,Soledad; Manieu,Catalina; López-Fenner,Julio; Ayarza,Eliana; Page,Jesús; González,Marisel; Manterola,Marcia; Fernández-Donoso,Raúl.
BACKGROUND: The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns of spatial associations among chromosomal domains from different bivalents. The meiotic nuclear architecture depends on the chromosome characteristics and consequently is prone to modification by chromosomal rearrangements. In this work, we consider Mus domesticus spermatocytes with diploid chromosome number 2n = 40, all telocentric, and investigate a possible modification of the ancestral nuclear architecture due to the emergence of derived Rb chromosomes, which may be present in the homozygous or heterozygous condition. RESULTS: In the 2n = 40 spermatocyte nuclei random associations mediated by pericentromeric heterochromatin among the 19 telocentric...
Tipo: Journal article
Palavras-chave: Robertsonian chromosomes; Mouse spermatocytes; Bivalent associations; Nuclear architecture.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100016

DNA Repair Genes XRCC1, XRCC3, XPD, and OGG1 Polymorphisms among the Central Region Population of Saudi Arabia
Provedor de dados: 48
Autores: Alanazi,Mohammad; Pathan,Akbar Ali Khan; Ajaj,Sana Abdulla; Khan,Wajahatullah; Shaik,Jilani P; Al Tassan,Nada; Parine,Narasimha Reddy.
DNA repair is one of the central defense mechanisms against mutagenic exposures. Inherited SNPs of DNA repair genes may contribute to variations in DNA repair capacity and susceptibility to cancer. Due to the presence of these variants, inter-individual and ethnic differences in DNA repair capacity have been established in various populations. Saudi Arabia harbors enormous genetic and cultural diversity. In the present study we aimed to determine the genotype and allele frequencies of XRCC1 Arg399Gln (rs25487), XRCC3 Thr241Met (rs861539), XPD Lys751Gln (rs13181), and OGG1 Ser326Cys (rs1052133) gene polymorphisms in 386 healthy individuals residing in the central region of Saudi Arabia and compare them with HapMap and other populations. The genotype and...
Tipo: Journal article
Palavras-chave: DNA Repair genes; Polymorphism; Saudi population; XRCC1; XRCC3; OGG1; XPD.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200007

Mesenchymal Stem Cell treatment for autoimmune diseases: a critical review
Provedor de dados: 48
Autores: Figueroa,Fernando E; Carrión,Flavio; Villanueva,Sandra; Khoury,Maroun.
Mesenchymal stem cells (MSCs) are now known to display not only stem cell multipotency, but also robust antiinflammatory and regenerative properties. After widespread in-vitro and in-vivo preclinical testing, autologous and allogeneic MSCs have been applied in a range of immune mediated conditions, including graft versus host disease, Crohn's disease, multiple sclerosis, refractory systemic lupus erythematosus and systemic sclerosis. Current data suggests that MSCs may not only replace diseased tissues, but also exert several trophic, regenerative and antiinflammatory effects. While the clinical outcome in case reports and phase I-II trials seems occasionally striking, these limited results point to the need to perform controlled multicenter trials. Future...
Tipo: Journal article
Palavras-chave: Mesenchymal stem cells; Autoimmune diseases; Cellular therapy.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300008

Ethyl acetate fraction from Angelica sinensis inhibits IL-1&amp;#946;-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production
Provedor de dados: 48
Autores: Lee,Won-Seok; Lim,Jin-Han; Sung,Myung-Soon; Lee,Eun-Gyeong; Oh,Yoo-Jeong; Yoo,Wan-Hee.
BACKGROUND: The root of Angelica sinensis (AS), also known as "Dang-gui," was a popular herbal medicine widely used in the treatment of gynecological diseases in China, Korea, and Japan for a long time. This study aimed to determine the effects of ethyl acetate fraction from Angelica sinensis (EAAS) on the interleukin-1&amp;#946; (IL-1&amp;#946;)-induced proliferation of rheumatoid arthritis synovial fibroblasts (RASFs), and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) 2, and prostaglandin E2 (PGE2), involved in articular bone and cartilage destruction, by RASFs. RESULTS: RASF proliferation was evaluated with cholecystokinin octapeptide (CCK-8) reagent in the presence of IL-1&amp;#946; with/without EAAS. Expression of MMPs, tissue...
Tipo: Journal article
Palavras-chave: Angelica sinensis COX IL-1&amp;#946; MMPs Rheumatoid arthritis (RA).
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100041

Cellular Signaling
Provedor de dados: 48
Autores: LEYTON,LISETTE; QUEST,ANDREW F. G.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200001

TAPCells, the Chilean dendritic cell vaccine against melanoma and prostate cancer
Provedor de dados: 48
Autores: Salazar-Onfray,Flavio; Pereda,Cristián; Reyes,Diego; López,Mercedes N.
Here we summarize 10 years of effort in the development of a biomedical innovation with global projections. This innovation consists of a novel method for the production of therapeutic dendritic-like cells called Tumor Antigen Presenting Cells (TAPCells®). TAPCells-based immunotherapy was tested in more than 120 stage III and IV melanoma patients and 20 castration-resistant prostate cancer patients in a series of phase I and I/II clinical trials. TAPCells vaccines induced T cell-mediated memory immune responses that correlated with increased survival in melanoma patients and prolonged prostate-specific antigen doubling time in prostate cancer patients. Importantly, more than 60% of tested patients showed a Delayed Type Hypersensitivity (DTH) reaction...
Tipo: Journal article
Palavras-chave: Dendritic Cells; Cancer Immunotherapy; Melanoma Vaccine; Technology Transfer; Biotechnology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400014

Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats
Provedor de dados: 48
Autores: AJDŽANOVI,VLADIMIR Z; ŠOŠI-JURJEVI,BRANKA T; FILIPÓVI,BRANKO R; TRIFUNOVI,SVETLANA L; BRKIC,DEJAN D; SEKULI,MILKA I; MILOSEVI,VERICA LJ.
The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. However, despite the beneficial effects on health, concern has been raised that this isoflavone also acts as an endocrine-disrupting chemical. The aim of this study was to examine the effects of genistein on immunohistomorphometric features of pituitary adrenocorticotropic cells (ACTH) and blood concentrations of ACTH and corticosterone in orchidectomized middle-aged male rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30mg/kg/day) was administered subcutaneously for...
Tipo: Journal article
Palavras-chave: ACTH cells; Corticosterone; Genistein; Middle-age; Orchidectomy; Rats.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100002

Structurally conserved C-RFa revealed prolactin releasing activity in vitro and gene expression changes in pituitary of seasonally acclimatized carp
Provedor de dados: 48
Autores: Romero,Alex; Montefusco-Siegmund,Rodrigo; Valenzuela,Guillermo; Kausel,Gudrun; Muller,Marc; Brauchi,Sebastian; Figueroa,Jaime.
Here we show the cloning and characterization of a novel homolog of prepro C-RFa cDNA from Cyprinus carpió. The deduced preprohormone precursor of 115 amino acids leads to a mature bioactive peptide of 20 amino acids with identical sequence to other teleost C-RFa. Modeling of the mature C-RFa peptide highlighted significant similarity to homologous human PrRP20, specifically the conserved amphipathic system defined by the C-terminal alpha-helix. Clearly, the synthetic C-RFa peptide stimulated prolactin release from primary cultured fish pituitary cells. For the first time, significant variation was shown in C-RFa mRNA and protein levels in the hypothalamus and pituitary between summer- and winter-acclimatized carp. Furthermore, C-RFa protein distribution...
Tipo: Journal article
Palavras-chave: C-RFa peptide; Prolactin releasing activity; Cyprinus carpió.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200011

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?
Provedor de dados: 48
Autores: GALLARDO-ESCÁRATE,CRISTIAN; ÁLVAREZ-BORREGO,JOSUÉ; VON BRAND,ELISABETH; DUPRÉ,ENRIQUE; DEL RÍO-PORTILLA,MIGUEL ÁNGEL.
In observations by confocal or conventional fluorescence microscopy, important factors should be considered in order to obtain accurate images. One of them, such as the fluorescence bleaching from highest intensity to lowest signal of fluorescence is a common problem with several DNA fluorochromes and especially for DAPI stain. The fluorescence of DAPI fades rapidly when it is exposed to UV light, under optimal conditions of observation. Although the fading process can be retarded using a mounting medium with antifading reagents, the photochemical process underlying the fluorescence decay has not yet been fully explained. In addition, no relationship between fluorescence fading and nuclear DNA content has been tested. In order to test this relationship, we...
Tipo: Journal article
Palavras-chave: Nuclear DNA content; Fluorescence fading; Image analysis; DAPI.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100004

Extended culture up to the blastocyst stage: a strategy to avoid multiple pregnancies in assisted reproductive technologies
Provedor de dados: 48
Autores: Sepúlveda,Soledad J; Portella,Jimmy R; Noriega,Luis P; Escudero,Ernesto L; Noriega,Luis H.
The aim of this study was to review the experience and outcomes of assisted reproduction cycles with embryos grown up to day 5 of development, comparing different parameters according to the ages of the patients. We retrospectively studied 1,874 assisted reproduction cycles where embryo culture was extended up to the fifth or sixth day of development. All IVF and ICSI cycles were included, comparing, according to patient age, the following rates: blastocyst formation, pregnancy, implantation and abortion. As control, we analyzed cycles with donated oocytes from young donors (OD). The number of embryos reaching the blastocyst stage is similar in all groups of patients. Only the OD group was different in terms of blastocyst formation, pregnancy and...
Tipo: Journal article
Palavras-chave: Blastocyst; Long-term culture; Pregnancy rate; Implantation rate.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200012

The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings
Provedor de dados: 48
Autores: FANTA,NORA; ORTEGA,XIMENA; PÉREZ,LUZ M.
In addition to phytoalexin synthesis, the defense response of intact Citrus limon seedlings against Alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (Ch) and ß-1,3-glucanases (Glu). A. alternata conidial germination was prevented by protein extracts from inoculated lemon seedlings, but also by extracts from mock-inoculated specimens. On the other hand, degradation of mycelia was accomplished only by protein extracts from inoculated seedlings. The presence of six Ch isoenzymes and of four Glu isoenzymes was detected in protein extracts from mock-inoculated seedlings. As a result of fungal inoculation, the isoenzyme pattern of Ch and Glu changed, making possible the detection of a new Ch isoenzyme and of three...
Tipo: Journal article
Palavras-chave: Alternaria alternata; Lemon; Hypersensitive response; Chitinases; Glucanases.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300012

Chloroplast localization of Cry1Ac and Cry2A protein- an alternative way of insect control in cotton
Provedor de dados: 48
Autores: Muzaffar,Adnan; Kiani,Sarfraz; Khan,Muhammad Azmat Ullah; Rao,Abdul Qayyum; Ali,Arfan; Awan,Mudassar Fareed; Iqbal,Adnan; Nasir,Idrees Ahmad; Shahid,Ahmad Ali; Husnain,Tayyab.
BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating the target protein at the point of insect attack will be more effective. It will not mean that the non-green parts of the plants are free of toxic proteins, but it will inflict more damage on the insects because they are at maximum activity in the green parts of plants. RESULTS: Successful cloning was achieved by the amplification of Cry2A, Cry1Ac, and a transit peptide. The appropriate polymerase chain reaction amplification and digested products confirmed...
Tipo: Journal article
Palavras-chave: Chloroplast transient peptide; Cry1Ac; Cry2A; Bt; CTP; Cry genes; Endotoxins; GUS; Transgenic plants.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100014

Upregulation of lncRNA BANCR associated with the lymph node metastasis and poor prognosis in colorectal cancer
Provedor de dados: 48
Autores: Shen,Xiaogang; Bai,Yifeng; Luo,Bin; Zhou,Xiaogang.
Abstract  Background  Growing evidence has supported that long non-coding RNAs (lncRNAs) could play vital roles in the development, progression, and prognosis of colorectal cancer (CRC). However, little is known about the clinical significance of BRAF-activated non-coding RNA (BANCR) in CRC. The aim of this study is to explore the clinical value of lncRNA BANCR in CRC patients.  Methods  The expression of lncRNA BANCR was measured in 106 CRC tissues and 65 adjacent normal tissues using the quantitative real-time PCR.  Results  The study showed that lncRNA BANCR was highly expressed in CRC tissues compared with adjacent normal tissues (P &lt; 0.001). In addition, high expression of lncRNA BANCR was positively correlated with the lymph node metastasis (P...
Tipo: Journal article
Palavras-chave: LncRNA; BANCR; Colorectal cancer; Lymph node metastasis; Prognosis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100223

Growth differentiation factor 9 and bone morphogenetic protein 15 expression in previtellogenic oocytes and during early embryonic development of Yellow-tail Kingfish Seriola lalandi
Provedor de dados: 48
Autores: Palomino,Jaime; Herrera,Giannina; Dettleff,Phillip; Martínez,Víctor.
BACKGROUND: During fish oocyte maturation, specific molecules are expressed and accumulated within oocyte until fertilization and embryo development. Special attention have been paid in members of the transforming growth factor (TGF-&amp;#946;) superfamily; growth differentiation factor 9 (GDF9/gdf9) and bone morphogenetic protein 15 (BMP15/bmp15), which exert regulatory functions during oocyte maturation and follicle development. However, little attention has been paid to the involvement of these molecules during embryogenesis considering its importance for the formation of a good quality egg and subsequent embryo survival. The purpose of this study was to analyze the expression of gdf9 andbmp15 in previtellogenic oocytes and during early embryonic...
Tipo: Journal article
Palavras-chave: Gdf9; Bmp15; Embryo development; Oocyte maturation; Fish.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100054

Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
Provedor de dados: 48
Autores: GARCÍA,KATHERINE P; RUBILAR,PAULINA S; VARGAS,MACARENA F; CÁRDENAS,HUGO; RIOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H ; FUHRER,JUAN; HECKELS,JOHN E; CHRISTODOULIDES,MYRON; VELÁSQUEZ,LUIS A.
In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase (LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS. Paradoxically, a reduction in NOS enzyme activity was observed in infected cultures, suggesting that gonococcal infection possibly influences translation of iNOS mRNA to the enzyme. In addition, treatment with the NOS inhibitor TRIM did not prevent gonococcal-induced cellular damage. In contrast, the addition of the inhibitor L-NAME induced a 40% reduction in LDH release,...
Tipo: Journal article
Palavras-chave: Cellular damage; Fallopian tubes; Neisseria gonorrhoeae; Nitric oxide.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100006

LIDERES MUNDIALES DE LA BIOLOGÍA DEL DESARROLLO SE REUNIERON EN SANTIAGO
Provedor de dados: 48
Autores: MAYOR,ROBERTO.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100005

Analysis of 5382insC (BRCA1) and 6174delT (BRCA2) mutations in 382 healthy Chilean women with a family history of breast cancer
Provedor de dados: 48
Autores: JARA,LILIAN; AMPUERO,SANDRA; SECCIA,LORENA; BUSTAMANTE,MARIO; BLANCO,RAFAEL; OJEDA,JOSÉ MANUEL.
Breast cancer is the most common malignancy among women. Chilean studies reveal that this cancer presents the third highest mortality rate. A family history of breast cancer is one of the major risk factors for the development of this disease. BRCA1 and BRCA2 are the two main hereditary breast cancer susceptibility genes, and mutations in these genes are related to inherited breast cancer. In specific populations only some mutations have been found to be associated with susceptibility. The purpose of this study was to establish the frequency of 5382insC (BRCA1) and 6174delT (BRCA2) germline mutations in 382 healthy Chilean women with at least two relatives affected with breast cancer and in probands and their relatives from 8 high risk families for breast...
Tipo: Journal article
Palavras-chave: Breast Cancer; BRCA1 and BRCA2 genes; Mutations; Chilean Women.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100011

On cognitive ecology and the environmental factors that promote Alzheimer disease: lessons from Octodon degus (Rodentia: Octodontidae)
Provedor de dados: 48
Autores: Rivera,Daniela S.; Inestrosa,Nibaldo C.; Bozinovic,Francisco.
Cognitive ecologist posits that the more efficiently an animal uses information from the biotic and abiotic environment, the more adaptive are its cognitive abilities. Nevertheless, this approach does not test for natural neurodegenerative processes under field or experimental conditions, which may recover animals information processing and decision making and may explain, mechanistically, maladaptive behaviors. Here, we call for integrative approaches to explain the relationship between ultimate and proximate mechanisms behind social behavior. We highlight the importance of using the endemic caviomorph rodent Octodon degus as a valuable natural model for mechanistic studies of social behavior and to explain how physical environments can shape social...
Tipo: Journal article
Palavras-chave: Cognitive ecology; Octodon degus; Social behavior; Stress; Aging; Alzheimer's disease.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100010

Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent
Provedor de dados: 48
Autores: GARCÍA,CARLOS; PEREIRA,PAULO; VALLE,LUIS; LAGOS,NÉSTOR.
Diarrhetic shellfish poisoning (DSP) is a gastrointestinal disease caused by lipid soluble polyether toxins produced by dinoflagellates and accumulated in shellfish. Diarrhetic shellfish poisoning is a worldwide threat to public health and the shellfish industry. To date, only four lipid soluble polyethers have been known as diarrhetic shellfish toxins. Among them, Okadaic acid (OA), Dinophysistoxin 1 (DTX-1, 35-methyl OA), Dinophysistoxin 2 (DTX-2, OA isomers) and Dinophysistoxin 3 (DTX-3, 7-O-acyl-35-methyl OA), all of which have free carboxilic groups. To perform quantitative analysis of DSP toxins in shellfish samples is a requirement, because DSP toxins are endemic in the Chilean mollusks of the southern regions, and although human symptoms of DSP...
Tipo: Journal article
Palavras-chave: DSP toxins; Okadaic acid; Dinophysistoxin-1 (DTX-1); Pyrenyldiazomethane (PDAM); Patagonian fjords; Chile.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200007

Apical Maxi-chloride channel from human placenta: 12 years after the first electrophysiological recordings
Provedor de dados: 48
Autores: RIQUELME,GLORIA.
The Maxi-chloride channel was the first ion channel described by electrophysiological methods in placenta. Because it is difficult to access a complex epithelium such as the placenta for electrophysiological procedures, the studies of ion channels from placental membranes have been performed only very recently. It was only in 1993 that a direct demonstration of a high-conductance chloride channel in apical membranes of intact trophoblastic epithelium was mentioned, and two years later, the description of this channel was reported from purified placental apical membranes reconstituted into artificial lipid membranes suitable for patch-clamp recordings. This brief review comments on the work done with regard to the electrophysiological characterization and...
Tipo: Journal article
Palavras-chave: Apical membrane; Chloride channel; Human placenta.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300006

UNA NUEVA ETAPA
Provedor de dados: 48
Autores: SANTOS,MANUEL J.
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500001

Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1&amp;#946;-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production
Provedor de dados: 48
Autores: Lee,Eun-Gyeong; Lee,Sang-ll; Chae,Han-Jung; Park,Seoung Ju; Lee,Yong Chul; Yoo,Wan-Hee.
Objectives: The objective of this study is to determine the effects of Ethyl acetate fraction from Cudrania tricuspidata (EACT) on the interleukin-1b (IL-1b)-induced proliferation of rheumatoid synovial fbroblasts (RASFs) and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) and prostaglandin E2 (PGE2) by RASFs. Materials and Methods: The proliferation of RASFs was evaluated with CCK-8 reagent in the presence of IL-1b with/without EACT. The expression of MMPs, TIMP-1, COXs, PGE2 and intracellular MAPK signalings, including p-ERK, p-p38, p-JNK and NF-kB were examined by immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and ELISA in conditions as described above. Results: EACT inhibits...
Tipo: Journal article
Palavras-chave: Cudrania tricuspidata; COX; IL-1b; MMPs; PGE2; Rheumatoid Arthritis (RA).
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200010

Abnormal Electrophysiological Activation in Schizophrenics during a Personal Traits Attribution Task
Provedor de dados: 48
Autores: SILVA,JAIME R; TORRES,WALTER M; ORTIZ,MANUEL S.
Changes in personal identity have been described as a major component in the expression of schizophrenia. Considering current evidence that relates the cortical structures of the medial line with the self, we hypothesize that there are differences in the frontomedial electrophysiological activation in schizophrenics when compared to normal subjects during tasks that require this function. In an experimental design, 9 schizophrenic and 7 normal subjects participated in a personal traits attribution task regarding the attribution of the self, another person and a simple reading. Electric brain activity was recorded with a dense array of 128 electrodes, with evoked brain potentials being obtained for the three conditions. Considering the evoked potential...
Tipo: Journal article
Palavras-chave: Schizophrenia; Event related potential; Self.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200003

Effect of iron on the activation of the MAPK/ERK pathway in PC12 neuroblastoma cells
Provedor de dados: 48
Autores: MUÑOZ,PABLO; ZAVALA,GABRIELA; CASTILLO,KAREN; AGUIRRE,PABLA; HIDALGO,CECILIA; NÚÑEZ,MARCO T.
Recent evidence suggests that reactive oxygen species function as second messenger molecules in normal physiological processes. For example, activation of N-Methyl-D-Aspartate receptor results in the production of ROS, which appears to be critical for synaptic plasticity, one of the cellular mechanisms that underlie learning and memory. In this work, we studied the effect of iron in the activation of MAPK/ERK pathway and on Ca2+ signaling in neuronal PC12 cells. We found that iron-dependent generation of hydroxyl radicals is likely to modulate Ca2+ signaling through RyR calcium channel activation, which, in turn, activates the MAPK/ERK pathway. These findings underline the relevance of iron in normal neuronal function.
Tipo: Journal article
Palavras-chave: NMDA receptor; Calcium; Iron; Oxidative stress; MAPK/ERK; Hippocampal slices; Long-term potentiation.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100021

Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells
Provedor de dados: 48
Autores: NAIR,MADHAVAN P; KANDASWAMI,CHITHAN; MAHAJAN,SUPRIYA; NAIR,HARIKRISHNA N; CHAWDA,RAM; SHANAHAN,THOMAS; SCHWARTZ,STANLEY A.
Flavonoids and related polyphenols, in addition to their cardioprotective, anti-tumor, anti-inflammatory, anti-carcinogenic and anti-allergic activities, also possess promising anti-HIV effects. Recent studies documented that the ß-chemokine receptors, CCR2b, CCR3 and CCR5, and the alpha-chemokine receptors, CXCR1, CXCR2 and CXCR4 serve as entry coreceptors for HIV-1. Although flavonoids and polyphenolic compounds elicit anti-HIV effects such as inhibition of HIV-1 expression and virus replication, the molecular mechanisms underlying these effects remain to be clearly elucidated. We hypothesize that flavonoids exert their anti-HIV effects, possibly by interfering at the HIV co-receptor level. We investigated the effect of flavonoid constituents of a...
Tipo: Journal article
Palavras-chave: PBMC; Peripheral blood mononuclear cells RT- PCR - reverse transcriptase polymerase chain reaction HIV-1; Human immunodeficiency virus-1 GSE; - Grape Seed Extract.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300016

A TRIBUTE TO FRANCISCO VARELA
Provedor de dados: 48
Autores: Bacigalupo,Juan; Palacios,Adrián.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100001

Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (Ovis aries) Genes - BCKDHA, NAGA and HEXA
Provedor de dados: 48
Autores: LIU,G. Y; GAO,S.Z.
The complete coding sequences of three sheep genes- BCKDHA, NAGA and HEXA were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR), based on the conserved sequence information of the mouse or other mammals. The nucleotide sequences of these three genes revealed that the sheep BCKDHA gene encodes a protein of 313 amino acids which has high homology with the BCKDHA gene that encodes a protein of 447 amino acids that has high homology with the Branched chain keto acid dehydrogenase El, alpha polypeptide (BCKDHA) of five species chimpanzee (93%), human (96%), crab-eating macaque (93%), bovine (98%) and mouse (91%). The sheep NAGA gene encodes a protein of 411 amino acids that has high homology with the alpha-N-acetylgalactosaminidase...
Tipo: Journal article
Palavras-chave: Sheep; BCKDHA; NAGA; HEXA; Tissue expression.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100007

Effect of Mediterranean and Occidental Diets, and Red Wine, on Plasma Fatty Acids in Humans: An Intervention Study
Provedor de dados: 48
Autores: URQUIAGA,INÉS; GUASCH,VIVIANA; MARSHALL,GUILLERMO; SAN MARTÍN,ALEJANDRA; CASTILLO,ÓSCAR; ROZOWSKI,JAIME; LEIGHTON,FEDERICO.
The type of diet consumed by individuals has been associated with the development of some chronic diseases, including cardiovascular disease (CVD), cancer, diabetes, and others. Populations that consume diets rich in fruits and vegetables and drink wine in moderation, as the Mediterranean, have a higher life expectancy and less chronic diseases than other occidental populations. We carried out an intervention study in humans to evaluate the effect of a Mediterranean diet (MD), an Occidental diet (OD) and their supplementation with red wine, on biochemical, physiological and clinical parameters related to atherosclerosis and other chronic diseases. For 3 months, two groups of 21 male volunteers each, received either a MD or an OD; during the second month,...
Tipo: Journal article
Palavras-chave: Cardiovascular disease; Chronic diseases; Fatty acids; Mediterranean diet; Monounsaturated fatty acids (MUFA); Occidental diet; W-6 fatty acids; W-3 fatty acids; Plasma fatty acids; Polyunsaturated fatty acids (PUFA); Saturated fatty acids (SFA); Wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200012

Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats
Provedor de dados: 48
Autores: Ahmed,Rasha R.; Mahmoud,Ayman; Ahmed,Osama M.; Metwalli,Ali; Ebaid,Hossam.
BACKGROUND: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-dia-betic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72) and keratin16 (Krt16) expression during wound healing in diabetic rats. METHODS: WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days. RESULTS: At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and...
Tipo: Journal article
Palavras-chave: Diabetic model; Whey protein; Heat shock protein-72; Keratin16; Wound healing.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100054

Cisgenesis and Intragenesis: New tools For Improving Crops
Provedor de dados: 48
Autores: Espinoza,C; Schlechter,R; Herrera,D; Torres,E; Serrano,A; Medina,C; Arce-Johnson,P.
Genetically Modified Organisms (GMO) could be the answer for many relevant problems affecting crops. However, improving crops through GMO is also often associated with safety concerns, environmental risks and health issues due to the presence of foreign DNA. These limitations have prompted the development of alternative technologies. Recently, cisgenesis and intragenesis have been developed as new tools aimed to modify crops. While cisgenesis involves genetic modification using a complete copy of natural genes with their regulatory elements that belong exclusively to sexually compatible plants, intragenesis refers to the transference of new combinations of genes and regulatory sequences belonging to that particular species. So far, application of...
Tipo: Journal article
Palavras-chave: Cisgenesis; Intragenesis; Grapevine promoters; VvGRIP24.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400003

Hereditary hemochromatosis: An opportunity for gene therapy
Provedor de dados: 48
Autores: EZQUER,FERNANDO; NÚÑEZ,MARCO T; ROJAS,ALEJANDRO; ASENJO,JUAN; ISRAEL,YEDY.
Levels of body iron should be tightly controlled to prevent the formation of oxygen radicals, lipoperoxidation, genotoxicity, and the production of cytotoxic cytokines, which result in damage to a number of organs. Enterocytes in the intestinal villae are involved in the apical uptake of iron from the intestinal lumen; iron is further exported from the cells into the circulation. The apical divalent metal transporter-1 (DMT1) transports ferrous iron from the lumen into the cells, while the basolateral transporter ferroportin extrudes iron from the enterocytes into the circulation. Patients with hereditary hemochromatosis display an accelerated transepithelial uptake of iron, which leads to body iron accumulation that results in cirrhosis, hepatocellular...
Tipo: Journal article
Palavras-chave: Iron; Intestine; Hemochromatosis; Gene therapy; HFE; DMT1; Cirrhosis.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100014

Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data
Provedor de dados: 48
Autores: Berthet,Nicolas; Descorps-Declère,Stéphane; Nkili-Meyong,Andriniaina Andy; Nakouné,Emmanuel; Gessain,Antoine; Manuguerra,Jean-Claude; Kazanji,Mirdad.
BACKGROUND: New sequencing technologies have opened the way to the discovery and the characterization of pathogenic viruses in clinical samples. However, the use of these new methods can require an amplification of viral RNA prior to the sequencing. Among all the available methods, the procedure based on the use of Phi29 polymerase produces a huge amount of amplified DNA. However, its major disadvantage is to generate a large number of chimeric sequences which can affect the assembly step. The pre-process method proposed in this study strongly limits the negative impact of chimeric reads in order to obtain the full-length of viral genomes. FINDINGS: Three different assembly softwares (ABySS, Ray and SPAdes) were tested for their ability to correctly...
Tipo: Journal article
Palavras-chave: RNA viral genome; Next generation sequencing; SPAdes; Assembling genome; Amplification with phi29 polymerase.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100039

Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans
Provedor de dados: 48
Autores: CHIUCHETTA,SIMONE JUREMA RUGGERI; CASTRO-PRADO,MARIALBA AVEZUM ALVES DE.
Vincristine is an alkaloid widely used as an antineoplastic agent. In eukaryotic cells the drug causes blockage in the G2 phase of the cell cycle and an increase in the frequency of sister chromatid exchanges. Due to the fact that germinating Aspergillus nidulans cells spend most of their cycle in G2 phase, they provide an excellent system for the study of mitotic crossing-over. Taking into account that mitotic crossing-over occurs during G2 period, the evaluation of recombinagenic and aneugenic potential of vincristine is provided with regard to two diploid strains of A. nidulans: a wild strain (uvsH+//uvsH+) and a defective one in DNA repair (uvsH//uvsH). Drug toxicity and its effect on the asexual cycle of A. nidulans has been evaluated as well....
Tipo: Journal article
Palavras-chave: Aneuploidy; Antineoplastic drug; Aspergillus nidulans; Recombinagenic activity.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100006

Antioxidant responses of cortex neurons to iron loading
Provedor de dados: 48
Autores: AGUIRRE,PABLA; MENA,NATALIA; TAPIA,VICTORIA; ROJAS,ALEJANDRO; ARREDONDO,MIGUEL; NÚÑEZ,MARCO TULIO.
Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in reduced and oxidized glutathione levels in response to a protocol of iron accumulation. We found that massive death occurred after 2 days in culture with 10 mM Fe. Surviving cells developed an adaptative response that included increased synthesis of GSH and the maintenance of a glutathione-based reduction potential. These results highlight the fundamental role of glutathione homeostasis in the antioxidant response and provide novel insights into the adaptative...
Tipo: Journal article
Palavras-chave: Glutathione; Iron; Redox response; Cortical neurons; Cell death.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100012

Zinc fingers: DNA binding and protein-protein interactions
Provedor de dados: 48
Autores: LEON,OSCAR; ROTH,MONICA.
The zinc finger domain is a very ubiquitous structural element whose hallmark is the coordination of a zinc atom by several amino acid residues (cysteines and histidines, and occasionally aspartate and glutamate). These structural elements are associated with protein-nucleic acid recognition as well as protein-protein interactions. The purpose of this review is to examine recent data on the DNA and protein binding properties of a few zinc fingers whose three dimensional structure is known
Tipo: Journal article
Palavras-chave: Zinc fingers; DNA binding; Protein-protein interactions.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100009

Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
Provedor de dados: 48
Autores: STANGE,CLAUDIA; FUENTES,PAULINA; HANDFORD,MICHAEL; PIZARRO,LORENA.
Carotenoids are synthesized in prokaryotic and eukaryotic organisms. In plants and algae, these lipophilic molecules possess antioxidant properties acting as reactive oxygen species scavengers and exert functional roles in hormone synthesis, photosynthesis, photomorphogenesis and in photoprotection. During the past decade almost all carotenogenic genes have been identified as a result of molecular, genetic and biochemical approaches utilizing Arabidopsis thaliana as the model system. Studies carried out in leaves and fruits of A. thaliana and tomato determined that light regulates carotenoid biosynthesis preferentially through the modulation of carotenogenic gene transcription. In this work we showed for the first time that light induces accumulation of...
Tipo: Journal article
Palavras-chave: Carotenoid biosynthesis; Carrot; Gene expression; Light regulation.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300006

MSC transplantation: a promising therapeutic strategy to manage the onset and progression of diabetic nephropathy
Provedor de dados: 48
Autores: Ezquer,Marcelo E; Ezquer,Fernando E; Arango-Rodríguez,Martha L; Conget,Paulette A.
Currently, one of the main threats to public health is diabetes mellitus. Its most detrimental complication is diabetic nephropathy (DN), a clinical syndrome associated with kidney damage and an increased risk of cardiovascular disease. Irrespective of the type of diabetes, DN follows a well-known temporal course. The earliest detectable signs are microalbuminuria and histopathological changes including extracellular matrix deposition, glomerular basement membrane thickening, glomerular and mesangial expansion. Later on macroalbuminuria appears, followed by a progressive decline in glomerular filtration rate and the loss of glomerular podocytes, tubulointerstitial fibrosis, glomerulosclerosis and arteriolar hyalinosis. Tight glycemic and hypertension...
Tipo: Journal article
Palavras-chave: Regenerative medicine; Diabetes mellitus; Diabetic nephropathy; Multipotent mesenchymal stromal cells; Mesenchymal stem cells.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300010

Calcium signaling: A historical account
Provedor de dados: 48
Autores: CARAFOLI,ERNESTO.
Tipo: Journal article
Palavras-chave: Calcium signaling; Calcium sensors; Calcium transporters.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400002

Signaling triggered by Thy-1 interaction with ß3 integrin on astrocytes is an essential step towards unraveling neuronal Thy-1 function
Provedor de dados: 48
Autores: AVALOS,ANA MARIA; LABRA,CECILIA V.; QUEST,ANDREW F.G.; LEYTON,LISSETTE.
Thy-1 is an abundant neuronal glycoprotein in mammals. Despite such prevalence, Thy-1 function remains largely obscure in the absence of a defined ligand. Recently described evidence that Thy-1 interacts with ß3 integrin on astrocytes will be discussed. Thy-1 binding to ß3 integrin triggers tyrosine phosphorylation of focal adhesion proteins in astrocytes, thereby promoting focal adhesion formation, cell attachment and spreading. Thy-1 has been reported to modulate neurite outgrowth by triggering a cellular response in neurons. However, our data indicate that Thy-1 can also initiate signaling events that promote adhesion of adjacent astrocytes to the underlying surface. Preliminary results suggest that morphological changes observed in the actin...
Tipo: Journal article
Palavras-chave: Thy-1 ligand/integrins; GPI-anchored proteins; Strocytes; Focal adhesion.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200015

Dynamics of tear fluid desiccation on a glass surface: a contribution to tear quality assessment
Provedor de dados: 48
Autores: Traipe-Castro,Leonidas; Salinas-Toro,Daniela; López,Daniela; Zanolli,Mario; Srur,Miguel; Valenzuela,Felipe; Cáceres,Aníbal; Toledo-Araya,Héctor; López-Solís,Remigio.
BACKGROUND: Fern-like crystalloids form when a microvolume of tear is allowed to dry out at ambient conditions on a glass surface. Presence of crystalloids in tear "microdesiccates" is used to evaluate patients with Dry-Eye disease. This study aims to examine morphologically the desiccation process of normal tear fluid and to identify changes associated with accelerated tear evaporation. Tear microdesiccates from healthy (Non-Dry Eye) and Dry Eye subjects were produced at ambient conditions. Microdesiccate formation was monitored continuously by dark-field video microscopy. Additionally, accelerated desiccation of tear samples from healthy subjects was conducted under controlled experimental conditions. Particular morphological domains of tear...
Tipo: Journal article
Palavras-chave: Tear; Tear ferning test; Dry eye; Dark-field microscopy.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100025

Infection and invasion mechanisms of Trypanosoma cruzi in the congenital transmission of Chagas' disease: A proposal
Provedor de dados: 48
Autores: Kemmerling,Ulrike; Bosco,Cleo; Galanti,Norbel.
Chagas' disease is produced by the haemophlagelated protozoan Trypanosoma cruzi and transmitted by haematophages insects such as Triatoma infestans (vinchuca). Due to vector control, congenital transmission gains importance and is responsible for the presence and expansion of this disease in non-endemic areas. The mechanisms of congenital infection are uncertain. It has been suggested that the parasite reaches the fetus through the bloodstream by crossing the placental barrier, and that congenital Chagas' disease is the result of complex interactions between the immune response, placental factors, and the parasite's characteristics. We review the cellular and molecular mechanisms of infection and invasion of the parasite and how immune and placental...
Tipo: Journal article
Palavras-chave: Congenital Chagas' disease; Mechanism of infection; Tissue invasion.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300007

Free Radical Scavenging Profile and Myeloperoxidase Inhibition of Extracts from Antidiabetic Plants: Bauhinia forficata and Cissus sicyoides
Provedor de dados: 48
Autores: KHALIL,NAJEH M; PEPATO,MARIA T; BRUNETTI,IGUATEMY L.
There is abundant evidence that reactive oxygen species are implicated in several physiological and pathological processes. To protect biological targets from oxidative damage, antioxidants must react with radicáis and other reactive species faster than biological substrates do. The aim of the present study was to determine the in vitro antioxidant activity of aqueous extracts from leaves of Bauhinia forficata Link (Fabaceae - Caesalpinioideae) and Cissus sicyoides L. (Vitaceae) (two medicinal plants used popularly in the control of diabetes mellitus), using several different assay systems, namely, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) decolorization, superoxide anión radical (0(2)•-) scavenging and myeloperoxidase (MPO) activity....
Tipo: Journal article
Palavras-chave: ABTS decolorization assay; Antioxidant activity; Superoxide anión scavenging activity.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200006

CHILE INVITA A CHILE: INSTITUTO MILENIO DE BIOLOGIA FUNDAMENTAL Y APLICADA ORGANIZA ORIGINAL ENCUENTRO DE ESTUDIANTES
Provedor de dados: 48
Autores: ROSEMBLATT,MARIO.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200007

Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line
Provedor de dados: 48
Autores: KUSNIER,CARLOS; CÁRDENAS,CÉSAR; HIDALGO,JORGE; JAIMOVICH,ENRIQUE.
Nuclear calcium appears to have an important role in the regulation of gene expression in many cells, but the mechanisms involved in controlling nuclear Ca2+ signaling are controversial and still poorly understood. We have described the presence of inositol 1,4,5 trisphosphate (IP3) receptors in the nuclei of skeletal muscle cells. Now, we have characterized the properties of the IP3 receptors channels present in the nuclei of the 1B5 cell line, which do not express any isoforms of the ryanodine receptor. Immunocytochemistry of isolated nuclei confirmed the presence of IP3R in the nuclear envelope and fluorescence measurements in nuclei suspensions allowed us to document ATP-dependent calcium loading by the nucleus and its release upon IP3 addition. Patch...
Tipo: Journal article
Palavras-chave: Single channel; Cell nucleus; IP3 receptor; Muscle.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300015

Regional differences in veins wall viscosity, compliance. energetics and damping: analysis of the pressure-diameter relationship during cyclical overloads
Provedor de dados: 48
Autores: ZÓCALO,YANINA; BIA,DANIEL; LLUBERAS,SEBASTIÁN; ARMENTANO,RICARDO L.
Background: The characterization of the dynamic process of veins walls is essential to understand venous functioning under normal and pathological conditions. However, little work has been done on dynamic venous properties. Aim: To characterize vein compliance (C), viscosity (&amp;#951;), peak-strain (W St) and dissipated (W D) energy, damping (&amp;#958;), and their regional differences in order to evalúate their role in venous functioning during volume-pressure overloads. Methods: In a mock circulation, pressure (P) and diameter (D) of different veins (anterior cava, jugular and femoral; from 7 sheep), were registered during cyclical volume-pressure pulses. From the P-D relationship, C, W St and &amp;#958; (at low and high P-D leveis), &amp;#951; and W D...
Tipo: Journal article
Palavras-chave: Compliance; Damping; Energetic; Vascular physiology; Venous wall; Viscosity.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200012

A micromethod for high throughput RNA extraction in forest trees
Provedor de dados: 48
Autores: LE PROVOST,GREGOIRE; HERRERA,RAÚL; PAIVA,JORGE AP; CHAUMEIL,PHILIPPE; SALIN,FRANCK; PLOMION,CHRISTOPHE.
A large quantity of high quality RNA is often required in the analysis of gene expression. However, RNA extraction from samples taken from woody plants is generally complex, and represents the main limitation to study gene expression, particularly in refractory species like conifers. Standard RNA extraction protocols are available but they are highly time consuming, and not adapted to large scale extraction. Here we present a high-throughput RNA extraction protocol. This protocol was adapted to a micro-scale by modifying the classical cetyltrimethylammonium (CTAB) protocol developed for pine: (i) quantity of material used (100-200 mg of sample), (ii) disruption of samples in microtube using a mechanical tissue disrupter, and (iii) the use of SSTE buffer....
Tipo: Journal article
Palavras-chave: RNA extraction; Micromethod; Forest trees.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400003

Distribution of delta sleep-inducing peptide in the newborn and infant human hypothalamus: an immunohistochemical study
Provedor de dados: 48
Autores: NAJIMI,MOHAMED; BENNIS,MOHAMED; MOYSES,EMMANUEL; CHIGR,FATIHA.
The distribution of delta sleep-inducing peptide immunoreactive cell bodies, fibers, and terminal-like structures was investigated in the normal human hypothalamus during the first postnatal year, using immunohistofluorescence and peroxidase anti-peroxidase techniques. Immunolabeled perikarya were relatively few and were mostly scattered through the anterior (preoptic) and mediobasal regions (infundibular nucleus) of the hypothalamus. DSIP-immunoreactive fibers and terminal-like fibers were observed throughout the entire rostro-caudal extent of the hypothalamus. They exhibit high densities in the preoptic region, the organum vasculosum of lamina terminalis, infundibular nucleus and median eminence. Moderate to low densities of DSIP-immunoreactive fibers...
Tipo: Journal article
Palavras-chave: DSIP; Immunohistochemistry; Development; Post-mortem; Human brain.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100013

The application of multiplex fluorimetric sensor for the analysis of flavonoids content in the medicinal herbs family Asteraceae, Lamiaceae, Rosaceae
Provedor de dados: 48
Autores: Sytar,Oksana; Bruckova,Klaudia; Hunkova,Elena; Zivcak,Marek; Konate,Kiessoun; Brestic,Marian.
BACKGROUND: The aim of our research work was to quantify total flavonoid contents in the leaves of 13 plant species family Asteraceae, 8 representatives of family Lamiaceae and 9 plant species belonging to familyRosaceae, using the multiplex fluorimetric sensor. Fluorescence was measured using optical fluorescence apparatus Multiplex(R) 3 (Force-A, France) for non-destructive flavonoids estimation. The content of total flavonoids was estimated by FLAV index (expressed in relative units), that is deduced from flavonoids UV absorbing properties. RESULTS: Among observed plant species, the highest amount of total flavonoids has been found in leaves ofHelianthus multiflorus (1.65 RU) and Echinops ritro (1.27 RU), Rudbeckia fulgida (1.13 RU) belonging to the...
Tipo: Journal article
Palavras-chave: Non-destructive measurement; Flavonoids; Multiplex fluorimetric sensor; Asteraceae; Lamiaceae; Rosaceae.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100005

ABSTRACTS OF THE POSTER PRESENTANTIONS
Provedor de dados: 48
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200020

Networks in Cognitive Systems and Biomedicine: Cerebral Processes, Models and Mathematical Tools Design
Provedor de dados: 48
Autores: COSMELLI,DIEGO; PALACIOS,ADRIAN G.
Convergence of clinical, empirical, methodological and theoretical approaches aimed at understanding the relation between brain function and cognition, is by now standard in most if not all academic programs in the area of Cognitive Science. This confederation of disciplines is one of the liveliest domains of inquiry and discussion into some of the most fundamental -and historically resilient- questions human beings have posed themselves. The contributions gathered in this special issue of Biological Research, directly inspired by the ongoing work at the Instituto de Sistemas Complejos de Valparaiso and the December 2006 CONICYT-INSERM-SFI workshop "Networks in Cognitive Systems / Trends and Challenge in Biomedicine: From Cerebral Process to Mathematical...
Tipo: Journal article
Palavras-chave: Cognitive Sciences; Brain Dynamics; Mind-Brain.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500002

CD4+ T cells in aged or thymectomized recipients of allogeneic stem cell transplantations
Provedor de dados: 48
Autores: Takahashi,Hiroshi; Ikeda,Kazuhiko; Ogawa,Kazuei; Saito,Syunnichi; Ngoma,Alain M; Mashimo,Yumiko; Ueda,Koki; Furukawa,Miki; Shichishima-Nakamura,Akiko; Ohkawara,Hiroshi; Nollet,Kenneth E; Ohto,Hitoshi; Takeishi,Yasuchika.
BACKGROUND: CD4+CD25highFOXP3+ regulatory T (Treg) cells, which include thymus-derived and peripherally induced cells, play a central role in immune regulation, and are therefore crucial to prevent graft-versus-host disease (GVHD). The increasing use of allogeneic hematopoietic stem cell transplantation (allo-HSCT) for elderly patients with thymus regression, and our case of allo-HSCT shortly after total thymectomy, raised questions about the activity of thymus-derived Treg cells and peripherally induced Treg cells, which are otherwise indistinguishable. RESULTS: We found that despite pre-transplant thymectomy or older age, both naïve and effector Treg cells, as well as naïve and effector conventional T cells, proliferated in allo-HSCT recipients. Higher...
Tipo: Journal article
Palavras-chave: Regulatory T cells; Allogeneic hematopoietic stem cell transplantation; Thymus; Graft-versus-host disease.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100041

First description of the immature stages of Hemilucilia segmentaria (Diptera: Calliphoridae)
Provedor de dados: 48
Autores: THYSSEN,PATRICIA J; LINHARES,ARÍCIO X.
The immature stages oí Hemilucilia segmentaria (Fabricius, 1805) (Diptera: Calliphoridae) are described. Egg morphology and structures such as the cephalopharyngeal skeleton, anterior and posterior spiracles, and the dorsal spines between the prothorax and mesothorax from first, second and third instar larvae are characterized, using light and scanning electron microscopy. This species is abundant in Neotropical forests and, because of its necrophagous behavior, is of substantial medico-legal importance for estimating the postmortem interval in criminal investigations. Information presented herein may be useful to differentiate among eggs and larvae of closely related species and to supplement the database for blowfly identification
Tipo: Journal article
Palavras-chave: Hemilucilia segmentaria; Calliphoridae; Immature stages; Forensic Entomology.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400001

Habituation of the eyeblink response in humans with stimuli presented in a sequence of incremental intensity
Provedor de dados: 48
Autores: Ponce,Fernando P; Quintana,Gonzalo R; Philominraj,Andrew S; Vogel,Edgar H.
In an experiment we examined whether the repeated presentation of tones of gradually increasing intensities produces greater decrement in the eyeblink reflex response in humans than the repetition of tones of constant intensities. Two groups of participants matched for their initial level of response were exposed to 110 tones of 100-ms duration. For the participants in the incremental group, the tones increased from 60- to 90- dB in 3-dB steps, whereas participants in the constant group received the tones at a fixed 90-dB intensity. The results indicated that the level of response in the last block of 10 trials, in which both groups received 90-dB tones, was significantly lower in the incremental group than in the constant group. These findings support the...
Tipo: Journal article
Palavras-chave: Habituation; Incremental stimulus intensity effect; Sensitization.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300011

Extraction of saponins and toxicological profile of Teucrium stocksianum boiss extracts collected from District Swat, Pakistan
Provedor de dados: 48
Autores: Shah,Syed Muhammad Mukarram; Sadiq,Abdul; Shah,Syed Muhammad Hassan; Khan,Shahzeb.
BACKGROUND: The current era is facing challenges in the management of neoplasia and weeds control. The currently available anti-cancer and herbicidal drugs are associated with some serious side effects. Therefore numerous researchers are trying to discover and develop plant based alternative particularly for the rational management of cancer and weed control. Teucrium stocksianum possess antioxidant and analgesic activities. The current study was designed to evaluate crude saponins (CS), methanolic extract and sub-fractions of T. stocksianum for cytotoxic and phytotoxic potentials. CS, methanolic extract and sub-fractions were extracted from powdered plant material using different solvents. Cytotoxic potential of the extracts at a dose of 10, 100 and 1000...
Tipo: Journal article
Palavras-chave: Saponins; Teucrium stocksianum; Cytotoxicity; Phytotoxicity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100059

Hematopoietic stem cell transplantation: clinical use and perspectives
Provedor de dados: 48
Autores: Barriga,Francisco; Ramírez,Pablo; Wietstruck,Angélica; Rojas,Nicolás.
Hematopoietic stem cell transplantation is the accepted therapy of choice for a variety of malignant and non-malignant diseases in children and adults. Initially developed as rescue therapy for a patient with cancer after high doses of chemotherapy and radiation as well as the correction of severe deficiencies in the hematopoietic system, it has evolved into an adoptive immune therapy for malignancies and autoimmune disorders. The procedure has helped to obtain key information about the bone marrow environment, the biology of hematopoietic stem cells and histocompatibility. The development of this new discipline has allowed numerous groups working around the world to cure patients of diseases previously considered lethal. Together with the ever growing...
Tipo: Journal article
Palavras-chave: Hematopoietic; Stem cells; Transplantation; Allogeneic.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300012

Betamethasone inhibits tumor development, microvessel density and prolongs survival in mice with a multiresistant adenocarcinoma TA3
Provedor de dados: 48
Autores: Garrido,Osvaldo; Letelier,René; Rosas,Carlos; Fuenzalida,Marcela; Ferreira,Arturo; Lemus,David.
Tumor resistance to traditional cancer treatments poses an important challenge to modern science. Thus, angiogenesis inhibition is an important emerging cancer treatment. Many drugs are tested and corticosteroids have shown interesting results. Herein we investigate the effect on microvessel density, survival time and tumoral volume of mice with TA3-MTX-R tumors. Twenty six mice were inoculated with lxlO6 tumor cells, 4-5 days after injection, six mice were injected with PBS (group A) and twenty mice were treated with p-met (group B). All animals from Group A died on day 22. Group B was divided into Bl (treated discontinued) and B2 (treated daily) and observed until day 88. All mice were processed for histo-immunohistochemical analysis and the blood...
Tipo: Journal article
Palavras-chave: Angiogenesis; Betamethasone treatment; Metastasis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300008

A tribute to Dr. Teresa Pinto-Hamuy
Provedor de dados: 48
Autores: Robles,Luis; Torrealba,Fernando; Santos,Manuel.
Tipo: Journal article
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400001

Variation in concentrations of major bioactive compounds in Prunella vulgaris L. related to plant parts and phenological stages
Provedor de dados: 48
Autores: Chen,Yuhang; Zhu,Zaibiao; Guo,Qiaosheng; Zhang,Lixia; Zhang,Xiaoming.
Prunella vulgaris L. (Labiatae) contains a variety of structurally diverse natural products, primarily rosmarinic acid (RA), ursolic acid (UA) and oleanolic acid (OA), which possess a wide array of biological properties. In the present study, P. vulgaris was harvested at three developmental stages (vegetative, full-flowering and mature-fruiting stages), dissected into stem and leaf tissues and assayed for chemical contents using high performance liquid chromatography. Significant changes in the concentrations of the major secondary metabolites (RA, UA and OA) were observed at the different development stages. The highest concentrations of RA, UA and OA were found at the full-flowering stage (15.83 mg/g dry weight (DW) RA, 1.77 mg/g DW UA and 0.65 mg/g DW...
Tipo: Journal article
Palavras-chave: Prunella vulgaris L.; Ontogenetic and morphogenetic variation; Rosmarinic acid; Ursolic acid; Oleanolic acid.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200009

Leishmanicidal activity of carvacrol-rich essential oil from Lippia sidoides Cham
Provedor de dados: 48
Autores: Farias-Junior,Paulo A; Rios,Marcos C; Moura,Tauanny A; Almeida,Roque P; Alves,Pericles B; Blank,Arie F; Fernandes,Roberta P M; Scher,Ricardo.
Leishamaniasis is a disease that affects more than 2 million people worldwide, whose causative agent is Leishmania spp. The current therapy for leishmaniasis is far from satisfactory. All available drugs, including pentavalent antimony, require parenteral administration and are potentially toxic. Moreover, an increase in clinical resistance to these drugs has been reported. In this scenario, plant essential oils used traditionally in folk medicine are emerging as alternative sources for chemotherapeutic compounds. In this study, in vitro leishmanicidal effects of a thymol- and a carvacrol-rich essential oil from leaves of Lippia sidoides Cham. were investigated. The essential oils were extracted and their constituents were characterized by gas...
Tipo: Journal article
Palavras-chave: Carvacrol; P-cymene; Essential oil composition; Leishmania chagasi; Lippia ssp.; Thymol; Verbenaceae.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400012

Expression of TRF2 and its prognostic relevance in advanced stage cervical cancer patients
Provedor de dados: 48
Autores: Ozden,Sevgi; Tiber,Pinar Mega; Ozgen,Zerrin; Ozyurt,Hazan; Serakinci,Nedime; Orun,Oya.
BACKGROUND: Telomeres are protective caps consisted of specific tandem repeats (5&amp;#8242;-TTAGGG-3&amp;#8242;). Shortening of telomeres at each cell division is known as "mitotic clock" of the cells, which renders telomeres as important regulators of lifespan. TRF2 is one of the critical members of shelterin complex, which is a protein complex responsible from the preservation of cap structure, and loss or mutation of TRF2 results in DNA damage, senescence or apoptosis. Since cancer is frequently associated with aberrant cell cycle progression, defective DNA repair or apoptosis pathways, TRF2 could be one likely candidate for cancer therapy. Here we investigated the prognostic role of TRF2 levels in cervical cancer patients. Fold-induction rates were...
Tipo: Journal article
Palavras-chave: Cervical cancer; Radiotherapy; Telomere repeat-binding factors 2; B-cell lymphoma-extra large; Apoptosis.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100055

For Francisco Varela: Explorer of the phenomenal world
Provedor de dados: 48
Autores: ROSCH,ELEANOR.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100003

Induction of programmed cell death by Prangos uloptera, a medicinal plant
Provedor de dados: 48
Autores: ZAHRI,SABER; RAZAVI,SEYED MEHDI; NIRI,FARSHAD HASSANZADEH; MOHAMMADI,SARIEH.
Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inhibitory and cell cytotoxicity effects of the extract were assayed by MTT and Trypan-blue tests, respectively. Morphological changes and DNA fragmentation were also evaluated. The viability tests showed 0.49 and 0.3 mg/ml as 50% inhibition concentration and 50% cytotoxicity concentration after 24 hours of treatment, respectively. Fluorescent microscopy analysis revealed chromatin fragmentation and scanning electron microscopy showed cell shrinkage and cytoplasmic...
Tipo: Journal article
Palavras-chave: Apoptosis; Cytotoxicity; McCoy; Prangos uloptera.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400013

A special issue devoted to STEM CELLS
Provedor de dados: 48
Autores: Santos,Manuel J; Ojeda,Patricio.
Tipo: Journal article
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300001

Dynamical Complexity in Cognitive Neural Networks
Provedor de dados: 48
Autores: GOLES,ERIC; PALACIOS,ADRIÁN G.
In the last twenty years an important effort in brain sciences, especially in cognitive science, has been the development of mathematical tool that can deal with the complexity of extensive recordings corresponding to the neuronal activity obtained from hundreds of neurons. We discuss here along with some historical issues, advantages and limitations of Artificial Neural Networks (ANN) that can help to understand how simple brain circuits work and whether ANN can be helpful to understand brain neural complexity
Tipo: Journal article
Palavras-chave: Artificial; Neural Net; Brain; Dynamical Complexity; Computational Neurosciences; Cellular Automata.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500009

LA FALTA DE UN ADECUADO RECONOCIMIENTO DE LAS CAPACIDADES EXISTENTES AFECTA EL DESARROLLO CIENTÍFICO EN CHILE
Provedor de dados: 48
Autores: KRAUSKOPF,MANUEL.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200001

Divergent effects of Nitric oxide on airway epithelial cell activation
Provedor de dados: 48
Autores: Neri,Tommaso; Conti,Ilaria; Cerri,Chiara; Tavanti,Laura; Paggiaro,Pierluigi; Celi,Alessandro.
Nitric oxide (NO*) is a gaseous mediator synthesized by Nitric oxide sinthases. NO* is involved in the modulation of inflammation, but its role in airway inflammation remains controversial. We investigated the role of NO* in the synthesis of the chemok Nes Interleukin-8 and Monocyte Chemotactic Protein-1, and of Intercellular Adhesion Molecule-1 by human airway epithelial cells. normal human bronchial epithelial cells and the bronchial epithelial cell line BEAS-2B were used. Neterleukin-8 (IL-8) and Monocyte Chemotactic Protein-1 (MCP-1) secretion and Intercellular Adhesion Molecule-1 (ICAM-1) expression were measured by ELISA. mRNA was assessed by semiquantitative RTI-PCR. Neterleukin-8 secretion was significantly reduced after 24h incubation with the NO*...
Tipo: Journal article
Palavras-chave: Adhesion molecules; Airway inflammation; Alveolar Epithelium; Chemok Nes; Nitric Oxide.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400012

Generation and analysis of expressed sequence tags from Botrytis cinerea
Provedor de dados: 48
Autores: SILVA,EVELYN; VALDÉS,JORGE; HOLMES,DAVID; SHMARYAHU,AMIR; VALENZUELA,PABLO D T.
Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and...
Tipo: Journal article
Palavras-chave: Botrytis cinerea; CDNA sequencing; Fungal pathogenicity.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018

Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants
Provedor de dados: 48
Autores: EHRENFELD,NICOLE; ROMANO,EDUARDO; SERRANO,CAROLINA; ARCE-JOHNSON,PATRICIO.
Potato leafroll virus (PLRV) is a major menace for the potato production all over the world. PLRV is transmitted by aphids, and until now, the only strategy available to control this pest has been to use large amounts of insecticides. Transgenic approaches involving the expression of viral replicases are being developed to provide protection for plants against viral diseases. The purpose of this study was to compare the protection afforded by the differential expression of PLRV replicase transgene in potato plants cv. Desirée. Plants were genetically modified to express the complete sense PLRV replicase gene. Two constructions were used, one containing the constitutive 35SCaMV promoter and the other the phloem-specific RolA promoter from Agrobacterium...
Tipo: Journal article
Palavras-chave: Replicase; PLRV; Replicase mediated resistance; RolA promoter; Transgenic potatoes.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100008

The cellular mechanisms of body iron homeostasis
Provedor de dados: 48
Autores: NUÑEZ,MARCO T; GARATE,MARCO A; ARREDONDO,MIGUEL; TAPlA,VICTORIA; MUÑOZ,PATRICIA.
Cells tightly regulate iron levels through the activity of iron regulatory proteins (IRPs) that bind to RNA motifs called iron responsive elements (IREs). When cells become iron-depleted, IRPs bind to IREs present in the mRNAs of ferritin and the transferrin receptor, resulting in diminished translation of the ferritin mRNA and increased translation of the transferrin receptor mRNA. Similarly, body iron homeostasis is maintained through the control of intestinal iron absorption. Intestinal epithelia cells sense body iron through the basolateral endocytosis of plasma transferrin. Transterrin endocytosis results in enterocytes whose iron content will depend on the iron saturation of plasma transferrin. Cell iron levels, in turn, inversely correlate with...
Tipo: Journal article
Palavras-chave: Metal ions; Intestinal absorption; Ferritin; Transferrin; Homeostasis.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200013

Assessment of the phytochemical constituents and antioxidant activity of a bloom forming microalgae Euglena tuba
Provedor de dados: 48
Autores: Chaudhuri,Dipankar; Ghate,Nikhil Baban; Deb,Shampa; Panja,Sourav; Sarkar,Rhitajit; Rout,Jayashree; Mandal,Nripendranath.
BACKGROUND: Unstable generation of free radicals in the body are responsible for many degenerative diseases. A bloom forming algae Euglena tuba growing abundantly in the aquatic habitats of Cachar district in the state of Assam in North-East India was analysed for its phytochemical contents, antioxidant activity as well as free radical scavenging potentials. RESULTS: Based on the ability of the extract in ABTS•+ radical cation inhibition and Fe3+ reducing power, the obtained results revealed the prominent antioxidant activity of the algae, with high correlation coefficient of its TEAC values to the respective phenolic and flavonoid contents. The extract had shown its scavenging activity for different free radicals and 41.89&amp;#8201;±&amp;#8201;0.41...
Tipo: Journal article
Palavras-chave: Euglenoid; Phytochemicals; Free radical scavenging; Antioxidant; Reducing power.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100024

Dynamic Causal Models and Autopoietic Systems
Provedor de dados: 48
Autores: DAVID,OLIVIER.
Dynamic Causal Modelling (DCM) and the theory of autopoietic systems are two important conceptual frameworks. In this review, we suggest that they can be combined to answer important questions about self-organising systems like the brain. DCM has been developed recently by the neuroimaging community to explain, using biophysical models, the non-invasive brain imaging data are caused by neural processes. It allows one to ask mechanistic questions about the implementation of cerebral processes. In DCM the parameters of biophysical models are estimated from measured data and the evidence for each model is evaluated. This enables one to test different functional hypotheses (i.e., models) for a given data set. Autopoiesis and related formal theories of...
Tipo: Journal article
Palavras-chave: Dynamic Causal Modelling; Brain functional organization; Plasticity; Autonomous systems; Autopoiesis.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500010

Nitric Oxide: Oxygen Radical Interactions in Atherosclerosis
Provedor de dados: 48
Autores: RUBBO,HOMERO; BATTHYANY,CARLOS; RADI,RAFAEL.
Atherosclerosis is one of the most common diseases and the principal cause of death in western civilization. The pathogenesis of this disease can be explained on the basis of the ‘oxidative-modification hypothesis,’ which proposes that low-density lipoprotein (LDL) oxidation represents a key early event. Nitric oxide (.NO) regulates critical lipid membrane and lipoprotein oxidation events by a) contributing to the formation of more potent secondary oxidants from superoxide (i.e.: peroxynitrite), and b) its antioxidant properties through termination reactions with lipid radicals to possibly less reactive secondary nitrogen-containing products (LONO, LOONO). Relative rates of production and steady state concentrations of superoxide and .NO and cellular sites...
Tipo: Journal article
Palavras-chave: Antioxidants; Free radicals; Lipid oxidation; Low density lipoprotein oxidation; Nitric oxide; Peroxynitrite.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200017

Long distance communication in the human brain: timing constraints for inter-hemispheric synchrony and the origin of brain lateralization
Provedor de dados: 48
Autores: ABOITIZ,FRANCISCO; LÓPEZ,JAVIER; MONTIEL,JUAN.
Analysis of corpus callosum fiber composition reveals that inter-hemispheric transmission time may put constraints on the development of inter-hemispheric synchronic ensembles, especially in species with large brains like humans. In order to overcome this limitation, a subset of large-diameter callosal fibers are specialized for fast inter-hemispheric transmission, particularly in large-brained species. Nevertheless, the constraints on fast inter-hemispheric communication in large-brained species can somehow contribute to the development of ipsilateral, intrahemispheric networks, which might promote the development of brain lateralization.
Tipo: Journal article
Palavras-chave: Axon; Brain lateralization; Corpus callosum; Inter-hemispheric transfer; Synchrony; Vision.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100007

Effects of weight loss on liver and erythrocyte polyunsaturated fatty acid pattern and oxidative stress status in obese patients with non-alcoholic fatty liver disease
Provedor de dados: 48
Autores: ELIZONDO,ALEJANDRA; ARAYA,JULIA; RODRIGO,RAMÓN; SIGNORINI,CINZIA; SGHERRI,CRISTIANA; COMPORTI,MARIO; PONIACHIK,JAIME; VIDELA,LUIS A.
Our aim was to study the influence of weight loss on the fatty acid (FA) composition of liver and erythrocyte phospholipids and oxidative stress status in obese, non-alcoholic, fatty liver disease (NAFLD) patients. Seven obese NAFLD patients who underwent subtotal gastrectomy with a gastro-jejunal anastomosis in roux and Y were studied immediately and 3 months after surgery. Seven non-obese patients who underwent anti-reflux surgery constituted the control group. Serum F2-isoprostane levels were measured by GS/NICI-MS/MS and FA composition was determined by GC. At the time of surgery, controls and obese patients exhibited a hepatic polyunsaturated fatty acid (PUFA) pattern that correlated with that of erythrocytes. Three months after surgery, NAFLD...
Tipo: Journal article
Palavras-chave: Polyunsaturated fatty acids; Oxidative stress; Obesity; Weight loss.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100008

Effects of R219K polymorphism of ATP-binding cassette transporter 1 gene on serum lipids ratios induced by a high-carbohydrate and low-fat diet in healthy youth
Provedor de dados: 48
Autores: Liu,Hui; Lin,Jia; Zhu,Xing Chun; Li,Yuan Hao; Fan,Mei; Zhang,Rong Rong; Fang,Ding Zhi.
BACKGROUND: Diets are the important players in regulating plasma lipid profiles. And the R219K polymorphism at the gene of ATP-binding cassette transporter 1(ABCA1) was reported to be associated with the profiles. However, no efforts have been made to investigate the changes of lipid profiles after a high-carbohydrate and low-fat diet in different subjects with different genotypes of this polymorphism. This study was to evaluate the effects of ABCA1 R219K polymorphism on serum lipid and apolipoprotein (apo) ratios induced by a high-carbohydrate/low-fat (high-CHO) diet. After a washout diet of 54.1% carbohydrate for 7 days, 56 healthy young subjects (22.89&amp;#8201;±&amp;#8201;1.80 years old) were given a high-CHO diet of 70.1% carbohydrate for 6 days....
Tipo: Journal article
Palavras-chave: Apolipoprotein; ATP-binding cassette transporter 1 gene; Gene polymorphism; High-carbohydrate/low-fat diet; Lipid ratios.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100004

Fuzzy subset approach in coupled population dynamics of blowflies
Provedor de dados: 48
Autores: CASTANHO,MARÍA J.P.; MAGNAGO,KARINE F; BASSANEZI,RODNEY C; GODOY,WESLEY A.C.
This paper is a study on the population dynamics of blowflies employing a density-dependent, non-linear mathematical model and a coupled population formalism. In this study, we investigated the coupled population dynamics applying fuzzy subsets to model the population trajectory, analyzing demographic parameters such as fecundity, survival, and migration. The main results suggest different possibilities in terms of dynamic behavior produced by migration in coupled populations between distinct environments and the rescue effect generated by the connection between populations. It was possible to conclude that environmental heterogeneity can play an important role in blowfly metapopulation systems. The implications of these results for population dynamics of...
Tipo: Journal article
Palavras-chave: Fuzzy logic; Blowflies; Coupled populations; Population biology.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200016

Muscle cell outside and inside: the nascent approach of a clinician
Provedor de dados: 48
Autores: FADIC,RICARDO.
Understanding muscle cell in disease and health is an unfinished process. Following the lead of Jaime Alvarez, I have had the opportunity of working on two complementary approaches to this field. One is the study of muscle cell surface molecules. Both synaptic muscle molecules, such as the asymmetric form of acetylcholinesterase, and extrasynaptic molecules, such as the extracellular matrix proteoglycans, are regulated by the motor nerve activity. This illustrates one of Jaime's teachings: cell phenotypes are a dynamic process that reflects the influence of other cells (&lt;A HREF="#0"&gt;Alvarez, 2001&lt;/A&gt;). Proteoglycans have many functions, including growth factor receptors. Studying them in muscular dystrophy will contribute to the comprehension...
Tipo: Journal article
Palavras-chave: Mitochondrial DNA; Muscle disease; Muscular dystrophy; Proteoglycans.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200018

Alpinia oxyphylla Miq. extract changes miRNA expression profiles in db-/db- mouse kidney
Provedor de dados: 48
Autores: Du,Guankui; Xiao,Man; Zhang,Xuezi; Wen,Maoyu; Pang,Chi; Jiang,Shangfei; Sang,Shenggang; Xie,Yiqiang.
Abstract  Background A number of dysregulated miRNAs have been identified and are proposed to have significant roles in the pathogenesis of type 2 diabetes mellitus or renal pathology. Alpinia oxyphylla has shown significant anti-inflammatory properties and play an anti-diabetes role. The objective of this study was to detect the alteration of miRNAs underlying the anti-diabetes effects of A. oxyphylla extract (AOE) in a type II diabetic animal model (C57BIKsj db-/db-).  Results Treatment with AOE for 8 weeks led to lower concentrations of blood glucose, urine albumin, and urine creatinine. 17 and 13 miRNAs were statistically identified as differentially regulated in the DB/DB and db-/db- AOE mice, respectively, compared to the untreated db-/db- mice. Of...
Tipo: Journal article
Palavras-chave: MiRNA; Db-/db- mice; Alpinia oxyphylla Miq; Kidney; Diabetic nephropathy.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100206

Departures from the physical optimality in the bronchial tree of rats (Rattus norvegicus)
Provedor de dados: 48
Autores: CANALS M,MAURICIO; ATALA,CRISTIAN; OLIVARES,RICARDO; NOVOA,FRANCISCO F; ROSENMANN,MARIO.
We studied the departure from the physical optimality of the bronchial tree of rats using both i) the minimum volume and power and ii) the minimum surface and drag criteria, considering the bronchial junction as the unit study based on Zamir's model for vascular trees. Our results show deviations of the junctions of the bronchial tree from the expected optimums in the proximal airway that can be explained by both, the turbulent or transitional flow regime, and the airway's necessity to distribute its terminal branches in the alveolar surface filling the thoracic volume. The departures of the observed values at the optimum for the minimum volume and power were significantly different than the obtained departure values for the minimum surface and drag...
Tipo: Journal article
Palavras-chave: Lung; Junction optimality; Optimization; Broncography.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300015

Effects of silk sericin on the proliferation and apoptosis of colon cancer cells
Provedor de dados: 48
Autores: Kaewkorn,Waraporn; Limpeanchob,Nanteetip; Tiyaboonchai,Waree; Pongcharoen,Sutatip; Sutheerawattananonda,Manote.
Sericin is a silk protein woven from silkworm cocoons (Bombyx mori). In animal model, sericin has been reported to have anti-tumoral action against colon cancer. The mechanisms underlying the activity of sericin against cancer cells are not fully understood. The present study investigated the effects of sericin on human colorectal cancer SW480 cells compared to normal colonic mucosal FHC cells. Since the size of the sericin protein may be important for its activity, two ranges of molecular weight were tested. Sericin was found to decrease SW480 and FHC cell viability. The small sericin had higher anti-proliferative effects than that of the large sericin in both cell types. Increased apoptosis of SW480 cells is associated with increased caspase-3 activity...
Tipo: Journal article
Palavras-chave: Silk sericin; Colon cancer; Apoptosis; Bcl-2; Caspase-3.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100006

Trans fatty acid isomers in human health and in the food industry
Provedor de dados: 48
Autores: VALENZUELA,ALFONSO; MORGADO,NORA.
Trans fatty acids are unsaturated fatty acids with at least one double bond in the trans configuration. These fatty acids occur naturally in dairy and other natural fats and in some plants. However, industrial hydrogenation of vegetable or marine oils is largely the main source of trans fatty acids in our diet. The metabolic effect of trans isomers are today a matter of controversy generating diverse extreme positions in light of biochemical, nutritional, and epidemiological studies. Trans fatty acids also have been implicated in the etiology of various metabolic and functional disorders, but the main concern about its health effects arose because the structural similarity of these isomers to saturated fatty acids, the lack of specific metabolic functions,...
Tipo: Journal article
Palavras-chave: Trans fatty acid isomers; Positional and geometric isomerization of fatty acids; Health effects of trans isomers; Hydrogenation and trans fatty acids; Conjugated linoleic acid.
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400007

Accumulation of Antioxidants in Apricot Fruit through Ripening: Characterization of a Genotype with Enhanced Functional Properties
Provedor de dados: 48
Autores: Hegedüs,Attila; Pfeiffer,Péter; Papp,Nóra; Abrankó,László; Blázovics,Anna; Pedryc,Andrzej; Stefanovits-Bányai,Éva.
Two apricot genotypes, 'Gonci magyarkajszi' and 'Preventa' were assayed at three ripening stages for flesh color indices (L*, a*, b*, C* and Hº), contents of total phenolics and vitamin C, and both water- and lipid-soluble antioxidant capacities (ferric reducing antioxidant power; 2,2'-diphenyl-1-picrylhydrazyl scavenging activity; total radical scavenging activity; and Photochem lipid-soluble antioxidant capacity) to compare their dynamics in the accumulation of antioxidant compounds and capacities through ripening. The increase in a*, b* and C* and decrease in Hº during ripening represented a color shift from green to yellow and orange due to carotenoid accumulation. Parallel to carotenoid accumulation, contents of total phenolics and vitamin C and...
Tipo: Journal article
Palavras-chave: Antioxidant capacity; Apricot; Color; Ripening; Total polyphenolic content; Vitamin C.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400004

Clinical and experimental approaches to knee cartilage lesion repair and mesenchymal stem cell chondrocyte differentiation
Provedor de dados: 48
Autores: Montoya,Fredy; Martínez,Fernando; García-Robles,María; Balmaceda-Aguilera,Carolina; Koch,Ximena; Rodríguez,Federico; Silva-Álvarez,Carmen; Salazar,Katterine; Ulloa,Viviana; Nualart,Francisco.
Cartilage has poor regeneration capacity due to the scarcity of endogenous stem cells, its low metabolic activity and the avascular environment. Repair strategies vary widely, including microfracture, autologous or allogenic tissue implantation, and in vitro engineered tissues of autologous origin. However, unlike the advances that have been made over more than two decades with more complex organs, including vascular, cardiac or bone tissues, similar advances in tissue engineering for cartilage repair are lacking. Although the inherent characteristics of cartilage tissue, such as the lack of vascularity and low cellular diversity, suggest that it would be one of the more simple tissues to be engineered, its functional weight-bearing role and implant...
Tipo: Journal article
Palavras-chave: Cartilage; Lesion; Microfracture; Stem cells.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400015

Redox regulation of calcium release in skeletal and cardiac muscle
Provedor de dados: 48
Autores: HIDALGO,CECILIA; ARACENA,PAULA; SANCHEZ,GINA; DONOSO,PAULINA.
In skeletal and cardiac muscle cells, specific isoforms of the Ryanodine receptor channels mediate Ca2+ release from the sarcoplasmic reticulum. These channels are highly susceptible to redox modifications, which regulate channel activity. In this work, we studied the effects of Ca2+ (endogenous agonist) and Mg2+ (endogenous inhibitor) on the kinetics of Ca2+ release from sarcoplasmic reticulum vesicles isolated from skeletal or cardiac mammalian muscle. Native skeletal vesicles exhibited maximal stimulation of release kinetics by 10-20 µM [Ca2+], whereas in native cardiac vesicles, maximal stimulation of release required only 1 µM [Ca2+]. In 10 µM [Ca2+], free [Mg2+] &lt; 0.1 mM produced marked inhibition of release from skeletal vesicles but free [Mg2+]...
Tipo: Journal article
Palavras-chave: Redox state; Ryanodine receptors; Sarcoplasmic reticulum; Calcium release kinetics; Mg2+ inhibition; S-nitrosoglutathione.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200009

Schizophrenia and reelin: a model based on prenatal stress to study epigenetics, brain development and behavior
Provedor de dados: 48
Autores: Negrón-Oyarzo,Ignacio; Lara-Vásquez,Ariel; Palacios-García,Ismael; Fuentealba,Pablo; Aboitiz,Francisco.
Schizophrenia is a severe psychiatric disorder that results in a significant disability for the patient. The disorder is characterized by impairment of the adaptive orchestration of actions, a cognitive function that is mainly dependent on the prefrontal cortex. This behavioral deficit, together with cellular and neurophysiological alterations in the prefrontal cortex, as well as reduced density of GABAergic cells and aberrant oscillatory activity, all indicate structural and functional deficits of the prefrontal cortex in schizophrenia. Among the several risk factors for the development of schizophrenia, stress during the prenatal period has been identified as crucial. Thus, it is proposed that prenatal stress induces neurodevelopmental alterations in the...
Tipo: Journal article
Palavras-chave: Schizophrenia; Prefrontal cortex; Prenatal stress; Functional connectivity; DNA methylation; Reelin.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100016

Local and global Ca2+ signals: physiology and pathophysiology
Provedor de dados: 48
Autores: PETERSEN,OLE H.
The pancreatic acinar unit is a classical example of a polarized tissue. Even in isolation, these cells retain their polarity, and this has made them particularly useful for Ca2+ signaling studies. In 1990, we discovered that this cell has the capability of producing both local cytosolic and global Ca2+ signals. The mechanisms underlying this signal generation have now been established. Furthermore, it has become clear that the local signals are sufficient for the control of both fluid and enzyme secretion, whereas prolonged global signals are dangerous and give rise to acute pancreatitis, a disease where the pancreas digests itself.
Tipo: Journal article
Palavras-chave: Pancreatic Acinar Cell; Intracellular Communication; Ca2+ Signal Compartmentalization; Ca2+ Release Mechanisms; Local Ca2+ Signals.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400023

Location matters: the endoplasmic reticulum and protein trafficking in dendrites
Provedor de dados: 48
Autores: Ramírez,Omar A; Härtel,Steffen; Couve,Andrés.
Neurons are highly polarized, but the trafficking mechanisms that operate in these cells and the topological organization of their secretory organelles are still poorly understood. Particularly incipient is our knowledge of the role of the neuronal endoplasmic reticulum. Here we review the current understanding of the endoplasmic reticulum in neurons, its structure, composition, dendritic distribution and dynamics. We also focus on the trafficking of proteins through the dendritic endoplasmic reticulum, emphasizing the relevance of transport, retention, assembly of multi-subunit protein complexes and export. We additionally discuss the roles of the dendritic endoplasmic reticulum in synaptic plasticity.
Tipo: Journal article
Palavras-chave: Endoplasmic reticulum; Protein trafficking; Dendrites; Neuron.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100003

Genital Sensory Stimulation Shifts Estradiol Intraoviductal Signaling from Nongenomic to Genomic Pathways, Independently from Prolactin Surges
Provedor de dados: 48
Autores: PEÑARROJA-MATUTAN0,C; PARADA-BUSTAMANTE,A; ORIHUELA,P A; RÍOS,M; VELASQUEZ,LUIS A; CROXATTO,H B.
Estradiol (E2) accelerates oviductal egg transport through nongenomic pathways involving oviductal protein phosphorylation in non-mated rats, and through genomic pathways in mated rats. Here we investigated the ability of cervico-vaginal stimulation (CVS) to switch the mode of action of E2 in the absence of other male-associated components. Pro-estrous rats were subjected to CVS with a glass rod and 12 hours later were injected subcutaneously with E2 and intrabursally with the RNA synthesis inhibitor Actinomycin D or the protein phosphorylation inhibitor H-89. The number of eggs in the oviduct, assessed 24 h later, showed that Actinomycin D, but not H-89 blocked the E2-induced egg transport acceleration. This clearly indicates that CVS alone, without other...
Tipo: Journal article
Palavras-chave: Mating; Oviduct; Estradiol; Signaling pathways; Prolactin receptor; Egg transport; Rat.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200012

A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification
Provedor de dados: 48
Autores: Zhang,Fanfan; Ye,Yu; Song,Deping; Guo,Nannan; Peng,Qi; Li,Anqi; Zhou,Xingrong; Chen,Yanjun; Zhang,Min; Huang,Dongyan; Tang,Yuxin.
Abstract  Background  Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed.  Results  In this study, we developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific for nucleocapsid gene to diagnose and monitor PDCoV infections. The detection limit of RT-LAMP assay was 1 × 101 copies of PDCoV, which was approximately 100-fold more sensitive than gel-based one-step reverse transcription polymerase chain...
Tipo: Journal article
Palavras-chave: Porcine Deltacoronavirus (PDCoV); RT-LAMP; Rapid diagnosis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100222

Genetic variants associated with neurodegenerative Alzheimer disease in natural models
Provedor de dados: 48
Autores: Salazar,Claudia; Valdivia,Gonzalo; Ardiles,Álvaro O.; Ewer,John; Palacios,Adrian G.
The use of transgenic models for the study of neurodegenerative diseases has made valuable contributions to the field. However, some important limitations, including protein overexpression and general systemic compensation for the missing genes, has caused researchers to seek natural models that show the main biomarkers of neurodegenerative diseases during aging. Here we review some of these models-most of them rodents, focusing especially on the genetic variations in biomarkers for Alzheimer diseases, in order to explain their relationships with variants associated with the occurrence of the disease in humans.
Tipo: Journal article
Palavras-chave: Degus; Genome; APP; APOE.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100014

Complex, multifocal, individual-specific attention-related cortical functional circuits
Provedor de dados: 48
Autores: BASILE,LUIS F. H.
Recent studies focusing on the analysis of individual patterns of non-sensory-motor CNS activity may significantly alter our view of CNS functional mapping. We have recently provided evidence for highly variable attention-related Slow Potential (SP) generating cortical areas across individuals (Basile et al., 2003, 2006). In this work, we present new evidence, searching for other physiological indexes of attention by a new use of a well established method, for individual-specific sets of cortical areas active during expecting attention. We applied latency corrected peak averaging to oscillatory bursts, from 124-channel EEG recordings, and modeled their generators by current density reconstruction. We first computed event-related total power, and averaging...
Tipo: Journal article
Palavras-chave: Attention; Cortical electrical activity; High-resolution electroencephalography; Slow potentials; Source localization; Functional mapping.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500007

CIENCIA, VINO Y SALUD
Provedor de dados: 48
Autores: Leighton,F.
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200001

Ethanol in low chronic dose level attenuates major organic effects in malnourished rats
Provedor de dados: 48
Autores: AGUIAR,ALINE S DE; BOAVENTURA,GILSON T; ABRAHÃO,RAFAEL F; FREITAS,THATIANA L; TAKIYA,CHRISTINA M; FILHO,PORPHIRIO J S; SILVA,VILMA A DA.
The aim of the present study was to evaluate the chronic toxicity of ethanol low blood levels in malnourished rats. Female Wistar rats (220 g) were subjected to either an ad libitum diet (W, well-nourished, n=10) or food restriction (M, malnourished, n=10). Water (WW and MW) or ethanol solution (W5% and M5%) was offered to half of each nutritional group (n=5) as the only fluid source. The treatment was continued for two months. After sacrifice, blood biochemical parameters and macroscopic, histologic and morphometric evaluation of the liver were performed. Results indicated that: Ethanol consumption was higher in malnourished rats and minimized body weight loss in malnourished rats, while it decreased the body weight gain in well-nourished ones. Behavioral...
Tipo: Journal article
Palavras-chave: Ethanol; Low dose; Malnutrition; Toxicity.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100004

Studies on parasitemia courses and mortality in mice infected with genetically distant Trypanosoma cruzi clonets
Provedor de dados: 48
Autores: WALLACE,ANNE; ORTÍZ,SILVIA; SÁNCHEZ,GITTITH; VILLAGRA,REBECA; MUGA,MABEL; SOLARI,ALDO.
The biological characterization of bloodstream forms of eleven Trypanosoma cruzi cloned stocks, corresponding to two genetically similar clonets (&lt;A HREF="#19"&gt;19&lt;/A&gt; and &lt;A HREF="#20"&gt;20&lt;/A&gt;) and one distant clonet (39), according to multilocus enzyme electrophoresis analysis, showed dissimilar parasitemia in an experimental isogenic mouse model. While clonet 39 stocks gave low parasitemias, clonets 19 or 20 stocks gave high parasitemias, independently of the inocula (10² and 10(4) bloodstream forms) used. High parasitemia did not always associate with greater mortality. Statistical studies on mortality using a low inocula showed significantly higher mortality with clonet 39 stocks when compared to clonets 19 or 20 stocks. Finally,...
Tipo: Journal article
Palavras-chave: Trypanosoma cruzi; Clonets; Parasitemia; Mortality; Bloodstream forms; Multilocus enzyme electrophoresis.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200011

Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm
Provedor de dados: 48
Autores: de los Reyes,Mónica; Palomino,Jaime; Martínez,Víctor; Aretio,Carolina; Gutiérrez,Michel.
We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm sample was incubated in canine capacitation medium (CCM) for 0, 1, 2 and 3 h at 20°C and at 37°C. After incubation, the samples were assessed by the indirect immunofluorescent staining technique. The probability of having unlabeled sperm (PUS), indicating acrosin loss, was modelled by a binomial distribution using logistic regression. There was a linear relationship between PUS and time at both temperatures (p&lt;0.001); however, a major percentage of unlabeled...
Tipo: Journal article
Palavras-chave: Acrosin; Capacitation; Dog sperm; Cryopreservation.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200005

Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
Provedor de dados: 48
Autores: Buchegger,Kurt; Ili,Carmen; Riquelme,Ismael; Letelier,Pablo; Corvalán,Alejandro H.; Brebi,Priscilla; Huang,Tim Hui-Ming; Roa,Juan Carlos.
BACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. METHODS: The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. RESULTS: In the cancer...
Tipo: Journal article
Palavras-chave: Reprimo; MDA-MB-231; Migration; Invasion.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100005

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio
Provedor de dados: 48
Autores: VERA,MARÍA INÉS; MOLINA,ALFREDO; PINTO,RODRIGO; REYES,MAURICIO; ÁLVAREZ,MARCO; KRAUSKOPF,ERWIN; QUEZADA,CLAUDIA; TORRES,JORGE; KRAUSKOPF,MANUEL.
The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression...
Tipo: Journal article
Palavras-chave: Ribosomal gene; RRNA; RDNA; Fish; Carp.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200014

Normalization of the levels of inflammatory molecules in Mycobacterium smegmatis-infected U937 cells by fibrate pretreatment
Provedor de dados: 48
Autores: Kim,Sung-Jo; Hong,Minho; Song,Ki Duk; Lee,Hak-Kyo; Ryoo,Sungweon; Heo,Tae-Hwe.
BACKGROUND: Tuberculosis (TB) is a respiratory tract disease caused by Mycobacterium tuberculosis infection. M. tuberculosis exploits immune privilege to grow and divide in pleural macrophages. Fibrates are associated with the immune response and control lipid metabolism through glycolysis with &amp;#946;-oxidation of fatty acids. RESULTS: In this study, we investigated the effect of fibrate pretreatment on the immune response during M. smegmatis infection in U937 cells, a human leukemic monocyte lymphoma cell line. The protein expression of tumor necrosis factor &amp;#945; (TNF-&amp;#945;), an inflammatory marker, and myeloid differentiation primary response gene 88 (MyD88), a toll like receptor adaptor molecule, in the infected group increased at 1 and 6...
Tipo: Journal article
Palavras-chave: Fibrate MyD88 TNF-&amp;#945; Tuberculosis.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100042

UNA BREVE RELEXIÓN SOBRE FINANCIAMIENTO DE LA INVESTIGACIÓN CIENTÍFICA, FORMACIÓN DE RECURSOS HUMANOS Y PLAN DE INNOVACIÓN Y DESARROLLO
Provedor de dados: 48
Autores: MONASTERIO,OCTAVIO.
Tipo: Journal article
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200001

Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli
Provedor de dados: 48
Autores: Muñoz,Yorka; Fuenzalida,Karen; Bronfman,Miguel; Gatica,Arnaldo; Sepúlveda,Marcelo; Bacigalupo,Juan; Roth,Alejandro D; Delgado,Ricardo.
Phototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere. These microvilli encompass the phototransduction proteins, most of which are transmembrane and exclusively rhabdomeric. Rhabdomere membrane lipids play a crucial role in the activation of the transient receptor potential ionic channels (TRP and TRPL) responsible for initiating the photoresponse. Despite its importance, rhabdomere lipid composition has not been established. We developed a novel...
Tipo: Journal article
Palavras-chave: Rhabdomere; Drosophila; Microvilli; Phototransduction; TRP channel; Fatty acid analysis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010

Increasing the rate of drying reduces metabolic imbalance, lipid peroxidation and critical water content in radicles of garden pea (Pisum sativum L.)
Provedor de dados: 48
Autores: Ntuli,Tobias M; Pammenter,Norman W; Berjak,Patricia.
Orthodox seeds become desiccation-sensitive as they undergo germination. As a result, germinating seeds serve as a model to study desiccation sensitivity in plant tissues. The effects of the rate of drying on the viability, respiratory metabolism and free radical processes were thus studied during dehydration and wet storage of radicles of Pisum sativum. For both drying regimes desiccation could be described by exponential and inverse modified functions. Viability, as assessed by germination capacity and tetrazolium staining, remained at 100% during rapid (&lt; 24 h) desiccation. However, it declined sharply at c. 0.26 g g¹ dm following slow (c. 5 days) drying. Increasing the rate of dehydration thus lowered the critical water content for survival. Rapid...
Tipo: Journal article
Palavras-chave: Antioxidant; Desiccation; Drying rate; Free radical processes; Lipid peroxidation; Metabolism; Respiration; Pisum sativum; Seed storage.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200002

Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses
Provedor de dados: 48
Autores: Simon,Thomas; Tanguy-Royer,Séverine; Royer,Pierre-Joseph; Boisgerault,Nicolas; Frikeche,Jihane; Fonteneau,Jean-François; Grégoire,Marc.
Dendritic cells (DCs) are professional antigen-presenting cells involved in the control and initiation of immune responses. In vivo, DCs exposed at the periphery to maturation stimuli migrate to lymph nodes, where they receive secondary signals from CD4+ T helper cells. These DCs become able to initiate CD8+ cytotoxic T lymphocyte (CTL) responses. However, in vitro investigations concerning human monocyte-derived DCs have never focused on their functional properties after such sequential maturation. Here, we studied human DC phenotypes and functions according to this sequential exposure to maturation stimuli. As first signals, we used TNF-&amp;#945;/polyI:C mimicking inflammatory and pathogen stimuli and, as second signals, we compared activated CD4+ T...
Tipo: Journal article
Palavras-chave: Cytotoxic T lymphocytes; Dendritic cells; T memory cell; Sequential maturation.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100005

Sonic Hedgehog in cancer stem cells: a novel link with autophagy
Provedor de dados: 48
Autores: Milla,Luis A; González-Ramírez,Claudia N; Palma,Verónica.
The Sonic Hegdehog/GLI (SHH/GLI) pathway has been extensively studied for its role in developmental and cancer biology. During early embryonic development the SHH pathway is involved mainly in pattern formation, while in latter stages its function in stem cell and progenitor proliferation becomes increasingly relevant. During postnatal development and in adult tissues, SHH/GLI promotes cell homeostasis by actively regulating gene transcription, recapitulating the function observed during normal tissue growth. In this review, we will briefly discuss the fundamental importance of SHH/GLI in tumor growth and cancer evolution and we will then provide insights into a possible novel mechanism of SHH action in cancer through autophagy modulation in cancer stem...
Tipo: Journal article
Palavras-chave: Cancer Stem Cells; Sonic Hedgehog; Cell Survival; Autophagy; Neuroblastoma; Cancer therapy.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300004

Uptake of DNA by cancer cells without a transfection reagent
Provedor de dados: 48
Autores: Kong,Yanping; Zhang,Xianbo; Zhao,Yongliang; Xue,Yanfang; Zhang,Ye.
Abstract  Background  Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will help improve the understanding of the metabolism of cancer. DNA is commonly packaged into a viral or lipid particle to be transferred into cells; this process is called transfection in laboratory. Cancer cells are known for having gene mutations and the evolving ability of endocytosis. Their uptake of DNAs might be different from normal cells; they may take in DNAs directly from the environment. In this report, we studied the uptake of DNAs in cancer cells without...
Tipo: Journal article
Palavras-chave: DNA uptake; Cancer cells; Endocytosis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100205

Intravenous administration of bone marrow-derived multipotent mesenchymal stromal cells has a neutral effect on obesity-induced diabetic cardiomyopathy
Provedor de dados: 48
Autores: Calligaris,Sebastián D; Conget,Paulette.
Obesity is a major global health issue. Obese patients develop metabolic syndrome, which is a cluster of clinical features characterized by insulin resistance and dyslipidemia. Its cardiac manifestation, diabetic cardiomyopathy, leads to heart failure. Bone marrow-derived multipotent mesenchymal stromal cells, also referred to as mesenchymal stem cells (MSC) are envisioned as a therapeutic tool not only for cardiovascular diseases but also for other degenerative conditions. Our aim was to evaluate whether the intravenous administration of MSC modifies cardiac dysfunction in obese mice. To this end, C57BL/6 mice were fed a regular (normal) or high-fat diet (obese). Obese animals received the vehicle (obese), a single dose (obese + 1x MSC) or three doses...
Tipo: Journal article
Palavras-chave: Obesity; Metabolic syndrome; Diabetic cardiomyopathy; Multipotent mesenchymal stromal cells; Cardiac function; Dobutamine.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300005

Genetic polymorphisms and asthma: findings from a case - control study in the Madeira island population
Provedor de dados: 48
Autores: Berenguer,Anabela Gonçalves; Fernandes,Ana Teresa; Oliveira,Susana; Rodrigues,Mariana; Ornelas,Pedro; Romeira,Diogo; Serrão,Tânia; Rosa,Alexandra; Câmara,Rita.
BACKGROUND: Asthma is a complex disease influenced by multiple genetic and environmental factors. While Madeira has the highest prevalence of asthma in Portugal (14.6%), the effect of both genetic and environmental factors in this population has never been assessed. We categorized 98 asthma patients according to the Global Initiative for Asthma (GINA) guidelines, established their sensitization profile, and measured their forced expiratory volume in 1second (FEV1) and forced vital capacity (FVC) indexes. Selected single nucleotide polymorphisms (SNPs) were analysed as potential markers for asthma susceptibility and severity in the interleukin 4 (IL4), interleukin 13 (IL13), beta-2-adrenergic receptor (ADRB2), a disintegrin and metalloprotease 33 (ADAM33),...
Tipo: Journal article
Palavras-chave: Asthma; Madeira; SNPs; Susceptibility; Severity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100040

Central actions of somatostatin in the generation and control of breathing
Provedor de dados: 48
Autores: Llona,Isabel; Eugenín,Jaime.
The neuropeptide somatostatin is involved in many functions in the central nervous system as well as in the periphery. When it is centrally injected, an irreversible apnea is often developed. In the present review, we discuss the effects of somatostatin as the result of its actions at three levels of the respiratory neural network: a) by modulating the output of cranial or spinal motoneurons; b) by altering the genesis of the respiratory rhythm in the brainstem; and c) by regulating the chemosensory drive input into the respiratory pattern generator
Tipo: Journal article
Palavras-chave: Apnea; Brainstem; Chemoreceptors; Respiratory rhythm.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400006

IP3 receptors and Ca2+ signals in adult skeletal muscle satellite cells in situ
Provedor de dados: 48
Autores: MOLGÓ,JORDI; COLASANTE,CESARE; ADAMS,DANY S; JAIMOVICH,ENRIQUE.
In this short article we review muscle satellite cell characteristics and our studies in adult rodent muscle satellite cells in situ. Using confocal laser scanning microscopy and immunocytochemistry, a high level of IP3 receptor (IP3R) immunostaining was detected in satellite cells. These cells were identified by their peripheral position, their size, the shape of their nucleus, the paucity of the apparent cytoplasm, and the immunostaining with specific molecular markers such as a-actinin, the neural cell adhesion molecule (N-CAM) and desmin. High extracellular K+ (60 mM) induced long-lasting Ca2+ signalsin satellite cells in situ. We suggest that electrical activity stimulates IP3-associated Ca2+ signals that could act in concert with signaling pathways...
Tipo: Journal article
Palavras-chave: Muscle satellite cells Inositol 1; 4; 5-trisphosphate receptors intracellular Ca2+ Signal transduction.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400019

Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
Provedor de dados: 48
Autores: FCOLLINS,JAMES.
Previous studies revealed novel genetic changes in the duodenal mucosa of iron-deprived rats during post-natal development. These observations are now extended to compare the genetic response to iron deficiency in the duodenum versus jejunum of 12-wk-old rats. cRNA samples were prepared from the duodenal and jejunal mucosa of three groups each of control and iron-deficient rats and hybridized with RAE 230A and 230B gene chips (Affymetrix). Stringent data reduction strategies were employed. Results showed that several genes were similarly induced in both gut segments, including DMT1, Dcytb, transferrin receptor 1, heme oxygenase 1, metallothionein, the Menkes copper ATPase (ATP7A), tripartitie motif protein 27, and the sodium-dependent vitamin C...
Tipo: Journal article
Palavras-chave: ATP7A; Copper transport; Iron-deficiency anemia; Microarray.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100004

Topography and axon arbor architecture in the visual callosal pathway: effects of deafferentation and blockade of TV-methyl-D-aspartate receptors
Provedor de dados: 48
Autores: OLAVARRÍA,JAIME F; LAING,ROBYN; HIROI,RYOKO; LASIENE,JÚRATE.
Visual callosal fibers link cortical loci in opposite hemispheres that represent the same visual field but whose locations are not mirror-symmetric with respect to the brain midline. Presence of the eyes from postnatal day 4 (P4) to P6 is required for this map to be specified. We tested the hypothesis that specification of the callosal map requires the activation of A'-methyl-D-aspartate receptors (NMDARs). Our results show that blockade of NMDARs with MK-801 during this critical period did not induce obvious abnormalities in callosal connectivity patterns, suggesting that retinal influences do not opérate through NMDAR-mediated processes to specify normal callosal topography. In contrast, we found that interfering with NMDAR function either through...
Tipo: Journal article
Palavras-chave: Corpus callosum; Interhemispheric commissure; Map; NMDAR; Striate cortex.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400007

Carbonic anhydrase activity in the red blood cells of sea level and high altitude natives
Provedor de dados: 48
Autores: GAMBOA,JORGE; CACEDA,RICARDO; GAMBOA,ALFREDO; MONGE-C,CARLOS.
Red blood cell carbonic anhydrase (CA) activity has not been studied in high altitude natives. Because CA is an intraerythocytic enzyme and high altitude natives are polycythemic, it is important to know if the activity of CA per red cell volume is different from that of their sea level counterparts. Blood was collected from healthy subjects living in Lima (150m) and from twelve subjects from Cerro de Pasco (4330m), and hematocrit and carbonic anhydrase activity were measured. As expected, the high altitude natives had significantly higher hematocrits than the sea level controls (p=0.0002). No difference in the CA activity per milliliter of red cells was found between the two populations. There was no correlation between the hematocrit and CA activity.
Tipo: Journal article
Palavras-chave: Carbonic anhydrase; Hypoxia; Polycythemia.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300006

LOS DERROTEROS CIENTÍFICOS DE FRANCISCO VARELA (1946-2001)
Provedor de dados: 48
Autores: LETELIER,JUAN CARLOS.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200002

Diffusion Signal in Magnetic Resonance Imaging: Origin and Interpretation in Neurosciences
Provedor de dados: 48
Autores: CHABERT,STEREN; SCIFO,PAOLA.
Diffusion Magnetic Resonance Imaging provides images of unquestionable diagnostic value. It is commonly used in the assessment of stroke and in white matter fiber tracking, among other applications. The diffusion coefficient has been shown to depend on cell concentration, membrane permeability, and cell orientation in the case of white matter or muscle fiber tracking; yet a clear relation between diffusion measurements and known physiological parameters is not established. The aim of this paper is to review hypotheses and actual knowledge on diffusion signal origin to provide assistance in the interpretation of diffusion MR images. Focus will be set on brain images, as most common applications of diffusion MRI are found in neuroradiology. Diffusion signal...
Tipo: Journal article
Palavras-chave: Diffusion; Magnetic Resonance Imaging; Interpretation; Non Gaussian.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500003

Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli
Provedor de dados: 48
Autores: PORCO,ANTONIETTA; GAMERO,ELIDA E; MYLONÁS,ELENA; ISTÚRIZ,TOMÁS.
Corynebacterium glutamicum is widely used in the industrial production of amino acids. We have found that this bacterium grows exponentially on a mineral médium supplemented with gluconate. Gluconate permease and Gluconokinase are expressed in an inducible form and, 6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3-fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions. Interestingly, these activities are lower than those detected in the strain Escherichia coli Ml-8, cultivated under similar conditions. Additionally, here we also confirmed that this bacterium lacks 6-phosphogluconate dehydratase activity. Thus, gluconate must be metabolized through the pentose phosphate pathway....
Tipo: Journal article
Palavras-chave: Corynebacterium glutamicum; Gluconate metabolism; Gnt.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300011

Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
Provedor de dados: 48
Autores: Guo,Jian-Ru; Chen,Qian-Qian; Lam,Christopher Wai-Kei; Zhang,Wei.
BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULTS: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin...
Tipo: Journal article
Palavras-chave: Karanjin; Cell cycle; Apoptosis; Cancer therapy.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040

Effectiveness of zinc in modulating perinatal effects of arsenic on the teratological effects in mice offspring
Provedor de dados: 48
Autores: Ahmad,Mohammad; Wadaa,Mohammad A. M; Farooq,Muhammad; Daghestani,Maha H; Sami,Ahmed S.
Exposure to arsenic via drinking water is considered as a worldwide problem. Studies have shown that arsenic exposure during pregnancy affects embryogenesis and offspring development in rats and mice. Zinc as a micronutrient regulates many physiological functions, including an antioxidative role under various toxic conditions. However, studies on the perinatal protective effect of zinc on offspring need further attention. The present study was designed to evaluate the potential protective role of zinc in mitigating the adverse effects in the offspring of arsenic exposure during pregnancy. The arsenic (40mg/kg body weight) and zinc (4% w/v) doses formed the only drinking fluid source for the experimental groups of dams during the perinatal period of the...
Tipo: Journal article
Palavras-chave: Arsenic; Zinc; Perinatal; Mice offspring; Sensory motor reflexes; Behavior; Oxidative stress.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200003

Finite size scaling in the local abundances of geographic populations
Provedor de dados: 48
Autores: Abades,Sebastián R; Marquet,Pablo A.
We analyzed the statistical distribution of intra-specific local abundances for a set North American breeding bird species. We constructed frequency plots for every species and found that they showed long-tail power-law behavior, truncated at an upper abundance cut-off value. Based on finite size scaling arguments, we investigated whether frequency curves may be considered scaled copies of each other. Data collapse was possible after taking powers of the total abundance of each species, in order to correct deviations from the underlying universal finite size scaling function (UFSS). The UFSS power law exponent oscillated in time within the regime of unbounded variance, which is consistent with the wild fluctuations that characterize ecological phenomena....
Tipo: Journal article
Palavras-chave: Finite size scaling; Geographic population; Data collapse; Truncated power-law; Bose-Einstein distribution; Scaling.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100013

Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens
Provedor de dados: 48
Autores: Graça-de Souza,Viviane K; Monteiro-Góes,Viviane; Manque,Patrício; Souza,Tatiana A.C.B; Corrêa,Paulo R.C.; Buck,Gregory A; Ávila,Andréa R; Yamauchi,Lucy M; Pinge-Filho,Phileno; Goldenberg,Samuel; Krieger,Marco A; Yamada-Ogatta,Sueli F.
The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22...
Tipo: Journal article
Palavras-chave: Phytomonas serpens; Trypanosoma cruzi; Antigenic cross-reactivity; Trypanosomatids.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200011

Mutations in BRCA1, BRCA2 and other breast and ovarian cancer susceptibility genes in Central and South American populations
Provedor de dados: 48
Autores: Jara,Lilian; Morales,Sebastian; Mayo,Tomas de; Gonzalez&amp;#8211;Hormazabal,Patricio; Carrasco,Valentina; Godoy,Raul.
Abstract Breast cancer (BC) is the most common malignancy among women worldwide. A major advance in the understanding of the genetic etiology of BC was the discovery of BRCA1 and BRCA2 (BRCA1/2) genes, which are considered high-penetrance BC genes. In non-carriers of BRCA1/2 mutations, disease susceptibility may be explained of a small number of mutations in BRCA1/2 and a much higher proportion of mutations in ethnicity-specific moderate- and/or low-penetrance genes. In Central and South American populations, studied have focused on analyzing the distribution and prevalence of BRCA1/2 mutations and other susceptibility genes that are scarce in Latin America as compared to North America, Europe, Australia, and Israel. Thus, the aim of this review is to...
Tipo: Journal article
Palavras-chave: Hereditary and early onset breast cancer; Susceptibility genes; Pathogenic point mutations; Large genomic rearrangements; Ethnic composition.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100505

Relationship between severity of adult community-acquired pneumonia and impairment of the antioxidant defense system
Provedor de dados: 48
Autores: Castillo,Rodrigo L; Carrasco,Rodrigo A; Álvarez,Pedro I; Ruiz,Mauricio; Luchsinger,Vivian; Zunino,Enna; Martínez,María A; Avendaño,Luis F.
Oxidant/antioxidant imbalance has been reported in some infectious diseases, including community-acquired pneumonia (CAP). The aim was to assess the antioxidant status in adults with CAP and its relationship with clinical severity at admission. Fifty-nine patients with CAP were enrolled and categorized at admission by the FINE score, from July 2010 to October 2012. In the same period 61 controls were enrolled. Plasma samples were obtained at admission for determination of the ferric reducing ability of plasma (FRAP) and lipid peroxidation (8-isoprostane). Erythrocyte reduced (GSH)/oxidized (GSSG) glutathione, malondialdehyde (MDA) and antioxidant enzyme activity were assessed. Antioxidant status in adults with CAP represented by FRAP and the GSH/GSSG ratio...
Tipo: Journal article
Palavras-chave: Pneumonia; Oxidative stress; Reduced glutathione; Lipid peroxidation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200013

Lipid peroxidation and antioxidants in hyperlipidemia and hypertension
Provedor de dados: 48
Autores: MORIEL,PATRICIA; PLAVNIK,FRIDA L; ZANELLA,MARIA T; BERTOLAMI,MARCELO C; ABDALLA,DULCINEIA SP.
Lipid peroxidation and lipid-derived oxidized products have been implicated in the pathogenesis of a variety of human diseases. To clarify the role of oxidative stress in essential hypertension and hypercholesterolemia the in vitro oxidative susceptibility of LDL, the antioxidant status and the lipid peroxide content of blood plasma were examined in hypercholesterolemic (HC), hypertensive (H), hypercholesterolemic/hypertensive (HH) and normolipidemic/normotensive subjects (N). Plasma ascorbate and lipid-soluble antioxidants were lower, while LDL oxidizability, CE-OOH and TL-OOH were higher in H, HC, and HH groups than in the N group. No difference was observed among groups for PL-OOH and isoprostanes. In summary, the results show that: 1) lipid- and...
Tipo: Journal article
Palavras-chave: Lipoprotein oxidation; Lipid peroxides; Isoprostanes; Antioxidants; Atherosclerosis; Oxidative stress.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200010

Vertical transmission of Trypanosoma cruzi in the Province of Choapa, IV Region, Chile: Preliminary Report (2005-2008)
Provedor de dados: 48
Autores: Apt,Werner; Zulantay,Inés; Solari,Aldo; Ortiz,Sylvia; Oddo,David; Corral,Gabriela; Truyens,Carine; Carlier,Yves.
Congenital Chagas disease acquired special importance in Chile after the certification of the control of Triatoma infestans and transmission by blood donors affected with Trypanosoma cruzi. In order to establish adequate protocols for intervention and control in infected mother-neonate pairs in endemic zones of Chagas disease, we present partial results (2005-2008) of a pilot project which is being carried out in the Province of Choapa, IV Region, Chile, whose objectives are: determine the current prevalence of the disease in pregnant women, estimate the incidence of vertical transmission of T. cruzi to newborns, determine the lineages of the parasite present in mothers who do and do not transmit the disease, determine the prevalence of Chagas disease in...
Tipo: Journal article
Palavras-chave: Congenital Chagas disease; Province of Choapa; Multidisciplinary study.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300002

Effects and molecular mechanism of chitosan-coated levodopa nanoliposomes on behavior of dyskinesia rats
Provedor de dados: 48
Autores: Cao,Xuebing; Hou,Dongzhi; Wang,Lei; Li,Sai; Sun,Shengang; Ping,Qineng; Xu,Yan.
BACKGROUND: Chitosan, the N-deacetylated derivative of chitin, is a cationic polyelectrolyte due to the presence of amino groups, one of the few occurring in nature. The use of chitosan in protein and drug delivery systems is being actively researched and reported in the literature RESULTS: In this study, we used chitosan-coated levodopa liposomes to investigate the behavioral character and the expression of phosphorylated extracellular signal-regulated kinase (ERK1/2), dopamine- and cAMP-regulated phos-phoprotein of 32 kDa (DARPP-32) and FosB/AFosB in striatum of rat model of levodopa-induced dyskinesia (LID). We found that scores of abnormal involuntary movement (AIM) decreased significantly in liposome group (P &lt; 0.05), compared with levodopa group....
Tipo: Journal article
Palavras-chave: Dyskinesia; Levodopa liposomes; ERK1/2; DARPP-32; FosB/AFosB.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100032

Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells
Provedor de dados: 48
Autores: Doan,Chung Chinh; Le,Long Thanh; Hoang,Son Nghia; Do,Si Minh; Van Le,Dong.
BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, leads on restricting cancer progression. The purpose of this study is to examine the therapeutic potential of dual gene targeted siRNA cocktail on human hepatocellular carcinoma Hep3B cells. RESULTS: The predesigned siRNAs could inhibit VEGF and KSP at mRNA level. siRNA cocktail showed a further downregulation on KSP mRNA and protein levels compared to KSP-siRNA or VEGF-siRNA, but not on VEGF expression. It also exhibited greater suppression on cell...
Tipo: Journal article
Palavras-chave: Vascular endothelial cell growth factor (VEGF); Kinesin spindle protein (KSP); SiRNA cocktail; Proliferation; Apoptosis; Hepatocellular carcinoma.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100064

Carotid body (Thermoreceptors, sympathetic neural activation, and cardiometabolic disease
Provedor de dados: 48
Autores: Iturriaga,Rodrigo; Del Rio,Rodrigo; Idiaquez,Juan; Somers,Virend K.
The carotid body (CB) is the main peripheral chemoreceptor that senses the arterial PO2, PCO2 and pH. In response to hypoxemia, hypercapnia and acidosis, carotid chemosensory discharge elicits reflex respiratory, autonomic and cardiovascular adjustments. The classical construct considers the CB as the main peripheral oxygen sensor, triggering reflex physiological responses to acute hypoxemia and facilitating the ventilatory acclimation to chronic hypoxemia at high altitude. However, a growing body of experimental evidence supports the novel concept that an abnormally enhanced CB chemosensory input to the brainstem contributes to overactivation of the sympathetic nervous system, and consequent pathology. Indeed, the CB has been implicated in several...
Tipo: Journal article
Palavras-chave: Autonomic dysfunction; Carotid body; Heart failure; Metabolic syndrome; Obstructive sleep apnea; Sympathetic activation.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100013

Immunohistochemical evidences showing the presence of thymulin containing cells located in involuted thymus and in peripheral lymphoid organs
Provedor de dados: 48
Autores: Folch,Hugo; Villegas,Juana V; Leyan,Víctor; Barría,Miguel; Eller,Gisela; Esquivel,Patricio.
Thymulin is a well-characterized thymic hormone that exists as a nonapeptide coupled to equimolar amounts of Zn2+. Thymulin is known to have multiple biological roles, including T cell differentiation, immune regulation, and analgesic functions. It has been shown that thymulin is produced by the reticulo-epithelial cells of the thymus, and it circulates in the blood from the moment of birth, maintain its serum level until puberty diminishing thereafter in life. To study the localization of this hormone, we prepared polyclonal and monoclonal antibodies against the commercial peptide and utilized immunocytochemical techniques for visualization. The results indicate that thymulin stains the thymic reticular cells, the outer layers of Hassall's corpuscles and...
Tipo: Journal article
Palavras-chave: Thymulin; Facteur Thymique Serique; Thymic hormones.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300005

Cadmium toxicity affects chlorophyll a and b content, antioxidant enzyme activities and mineral nutrient accumulation in strawberry
Provedor de dados: 48
Autores: Muradoglu,Ferhad; Gundogdu,Muttalip; Ercisli,Sezai; Encu,Tarik; Balta,Fikri; Jaafar,Hawa ZE; Zia-Ul-Haq,Muhammad.
BACKGROUND: Cadmium (Cd) is well known as one of the most toxic metals affecting the environment and can severely restrict plant growth and development. In this study, Cd toxicities were studied in strawberry cv. Camarosa using pot experiment. Chlorophyll and malondialdehyde (MDA) contents, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) activities and mineral nutrient concentrations were investigated in both roots and leaves of strawberry plant after exposure Cd. RESULTS: Cd content in both roots and leaves was increased with the application of increasing concentrations of Cd. We found higher Cd concentration in roots rather than in leaves. Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased...
Tipo: Journal article
Palavras-chave: Antioxidant enzymes; Cadmium; Chlorophyll; Heavy metal stress; Strawberry.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100011

Development and characterization of two new cell lines from common carp, Cyprinus carpio (Linn)
Provedor de dados: 48
Autores: Lakra,Wazir S; Goswami,M; Rajaswaminathan,T; Rathore,Gaurav.
Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10% FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100...
Tipo: Journal article
Palavras-chave: Cyprinus carpio; Cell line; MtDNA.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400002

Regulation of Rho Family GTPases by Cell-Cell and Cell-Matrix Adhesion
Provedor de dados: 48
Autores: ARTHUR,WILLIAM T.; NOREN,NICOLE K.; BURRIDGE,KEITH.
Integrins and cadherins are transmembrane adhesion receptors that are necessary for cells to interact with the extracellular matrix or adjacent cells, respectively. Integrins and cadherins initiate signaling pathways that modulate the activity of Rho family GTPases. The Rho proteins Cdc42, Rac1, and RhoA regulate the actin cytoskeleton. Cdc42 and Rac1 are primarily involved in the formation of protrusive structures, while RhoA generates myosin-based contractility. Here we examine the differential regulation of RhoA, Cdc42, and Rac1 by integrin and cadherin signaling. Integrin and cadherin signaling leads to a decrease in RhoA activity and activation of Cdc42 and Rac1. When the normal RhoA suppression is antagonized or RhoA signaling is increased, cells...
Tipo: Journal article
Palavras-chave: Integrins; Cadherins; Adhesion; Rho; Rac; Cdc42.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200016

A roadmap to directed enzyme evolution and screening systems for biotechnological applications
Provedor de dados: 48
Autores: Martínez,Ronny; Schwaneberg,Ulrich.
Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fine chemicals. The ever-growing demand for enzymes in increasingly specific applications requires tailoring naturally occurring enzymes to the non-natural conditions found in industrial processes. Relationships between enzyme sequence, structure and activity are far from understood, thus hindering the capacity to design tailored biocatalysts. In the field of protein engineering, directed enzyme evolution is a powerful algorithm to generate and identify novel and improved enzymes through iterative rounds of mutagenesis and screening applying a specific evolutive pressure. In practice, critical checkpoints in directed evolution...
Tipo: Journal article
Palavras-chave: Directed evolution; Enzyme engineering; High throughput screening; Random mutagenesis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400011

A critical assessment of the consciousness by synchrony hypothesis
Provedor de dados: 48
Autores: CANALES,ANDRÉS F; GÓMEZ,DAVID M; MAFFET,CLAUDIO R.
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500012

EL FUTURO DE ESPAÑA DEPENDE DE LA CIENCIA
Provedor de dados: 48
Autores: GUINOVART,JOAN J.
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100001

Parasexuality in asexual development mutants of Aspergillus nidulans
Provedor de dados: 48
Autores: ALEXANDRINO BECKER,TÂNIA CRISTINA; ALVES DE CASTRO-PRADO,MARIALBA A.
The parasexual cycle with parameiosis has been characterized previously by the occurrence of genetic recombination and haploidization inside heterokaryotic hyphae prior to conidial formation. The aim of current research was to characterize, through genetic and cytological analyses, an asexual development mutant strain of A. nidulans and to use it to obtain parameiotic segregants. Analyses showed the medusa phenotype of the B84 strain, whose mutant allele was mapped in the chromosome I. The heterokaryons B84(med)//G422(med+) and B84(med)//G839(brl) were formed in liquid MM+2% CM and inoculated in the appropriate selective media. Two mitotic segregant groups were obtained: aneuploids and haploid stable recombinants. Mitotic segregants, wild-types, and...
Tipo: Journal article
Palavras-chave: Parasexual cycle; Conidiation mutants; Mitotic recombination; Haploid recombinants.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200012

Neuroanatomical basis of behavioral disturbances in patients with prefrontal lesions
Provedor de dados: 48
Autores: SLACHEVSKY,ANDREA; PEÑA,MARCELA; PÉREZ,CAROLINA; BRAVO,EDUARDO; ALEGRÍA,PATRICIA.
The role of the frontal lobe in control of behavioral and cognitive abilities is explored in a group of 34 patients with brain lesions restricted to the prefrontal cortex. The scores in both structured behavioral questionnaires and standard neuropsychological tests were analyzed using the injured area of the frontal lobe as the independent variable. Our results show that patients with simultaneous lesions in supero_ and inferomedial areas of the prefrontal cortex exhibit higher behavioral disturbances. Bilateral lesions also are associated with greater behavioral troubles. On the contrary, cognitive abilities are globally impaired in prefrontal patients. Results are discussed in relation to current models of the organization of the prefrontal cortex and...
Tipo: Journal article
Palavras-chave: Dysexecutive syndrome; Prefrontal lesions; Behavior.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200006

Free radical chemistry in biological systems
Provedor de dados: 48
Autores: VALDEZ,LAURA B; LORES ARNAIZ,SILVIA; BUSTAMANTE,JUANITA; ALVAREZ,SILVIA; COSTA,LIDIA E; BOVERIS,ALBERTO.
Mitochondria are an active source of the free radical superoxide (O2-) and nitric oxide (NO), whose production accounts for about 2% and 0.5% respectively, of mitochondrial O2 uptake under physiological conditions. Superoxide is produced by the auto-oxidation of the semiquinones of ubiquinol and the NADH dehydrogenase flavin and NO by the enzymatic action of the nitric oxide synthase of the inner mitochondrial membrane (mtNOS). Nitric oxide reversibly inhibits cytochrome oxidase activity in competition with O2. The balance between NO production and its utilization results in a NO intramitochondrial steady-state concentration of 20-50 nM, which regulates mitochondrial O2 uptake and energy supply. The regulation of cellular respiration and energy production...
Tipo: Journal article
Palavras-chave: Superoxide radical; Nitric oxide; Peroxynitrite; Mitochondria; Apoptosis; Aging.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200005

Sexual orientation, handedness, sex ratio and fetomaternal tolerance-rejection
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
Fraternal birth order (FBO) appears as a prenatal cause of 15% of homosexual males (gays) through mnemonic maternal anti-male factors. Non-right-handed men seem to be protected from homosexuality. Four hypotheses are proposed: (1) androgenic factors of non-right-handedness neutralize anti-male factors; (2) non-right-handedness and homosexuality are lethal or produce mental impairment; (3) non-right-handed male embryos are insensitive to anti-male factors; (4) mothers of non-right-handed fetuses do not produce anti-male factors. Studies of the sex ratio (SR) of older and younger siblings show: (1) a significant heterogeneity in the SR of siblings of right or non-right handed heterosexual men and women; (2) lesbians are born among siblings with high SR; (3)...
Tipo: Journal article
Palavras-chave: Homosexuality; Handedness; Sex ratio; Birth order; Fetomoternal tolerance; Genetic distortions.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300012

Overview on chemotaxis and acid resistance in Helicobacter pylori
Provedor de dados: 48
Autores: VALENZUELA,MANUEL; CERDA,OSCAR; TOLEDO,HÉCTOR.
Tipo: Journal article
Palavras-chave: Helicobacter pylori; Acid tolerance response; Chemotaxis; Urease.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300014

Benzothiadiazole (BTH) induces resistance to Pepper golden mosaic virus (PepGMV) in pepper (Capsicum annuum L.)
Provedor de dados: 48
Autores: Trejo-Saavedra,D.L; García-Neria,M.A; Rivera-Bustamante,R.F.
Pepper is an economically important crop in many countries around the world but it is susceptible to many diseases. In Mexico, diseases caused by bipartite begomoviruses have emerged as important problems in pepper. Several control strategies have been explored wiht little success; most of them are based on the avoidance of virus transmission and the breeding for resistance. Abiotic inducers can act at various points in the signaling pathways involved in disease resistance, providing long-lasting, wide-spectrum resistance. Benzothiadiazole (BTH) shares the property of activating the systemic acquired resistance pathway downstream from the SA signaling. In this work, resistance to PepGMV infection was induced in pepper plants by activating the SA pathway...
Tipo: Journal article
Palavras-chave: Geminivirus; Systemic acquired resistance; Induced resistance; BTH.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400004

Supramolecular complex formation in cell signaling and disease: an update on a recurrent theme in cell life and death
Provedor de dados: 48
Autores: LEYTON,LISETTE; QUEST,ANDREW F.G.
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100004

Chile Ciencia 2000
Provedor de dados: 48
Tipo: Journal article
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400001

Age-related changes in quality and fertility of porcine semen
Provedor de dados: 48
Autores: Tsakmakidis,Ioannis A; Khalifa,Tarek AA; Boscos,Costas M.
The aim of this study was to investigate the effect of boar age on quality traits and fertility of liquid-stored semen. Boars were allocated into 3 age groups: 7-10 months (young), 18-33 months (mature), 51-61 months (old). Ejaculates of &amp;gt; 200x10(6) sperm/ml and 85% total motile sperm were extended to 30x10(6) sperm/ml, stored at 17-18 °C and used within 12-24 h for artificial insemination (AI) of 2062 multiparous sows. After 24 h of storage, aliquots of diluted semen were assessed for sperm progressive motility (SPM), incidence of sperm chromatin instability (SCI), proportion of live morphologically normal sperm (LMNS) and head morphometry of LMNS. The results showed that young boars had higher percentages of SCI and lower proportions of LMNS than...
Tipo: Journal article
Palavras-chave: Boar age; Boar semen; Morphology; Morphometry; Sperm chromatin.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400009

Effects of different water management options and fertilizer supply on photosynthesis, fluorescence parameters and water use efficiency of Prunella vulgaris seedlings
Provedor de dados: 48
Autores: Chen,Yuhang; Liu,Li; Guo,Qiaosheng; Zhu,Zaibiao; Zhang,Lixia.
BACKGROUND: Prunella vulgaris L. is a medical plant cultivated in sloping, sun-shaded areas in China. Recently, owing to air-environmental stress, especially drought stress strongly inhibits plant growth and development, the appropriate fertilizer supply can alleviate these effects. However, these is little information about their effects on P. vulgaris growing in arid and semi-arid areas with limited water and fertilizer supply. RESULTS: In this study, water stress decreased the photosynthetic pigment contents, inhibited photosynthetic efficiency, induced photodamage in photosystem 2 (PS2), and decreased leaf instantaneous WUE (WUEi). The decreased net photosynthetic rate (Pn) under medium drought stress compared with the control might result from...
Tipo: Journal article
Palavras-chave: Prunella vulgaris L N; P and K fertilizer Drought Photosynthesis Water use efficiency.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100012

The autonomous axon: a model based on local synthesis of proteins
Provedor de dados: 48
Autores: ALVAREZ,JAIME.
In the current understanding of axons, axoplasmic synthesis of proteins is negated, and it is asserted that proteins are transported from perikarya to axons. This 'transport model' in which axons are fully dependent of perikarya is seriously flawed. The 'autonomous axon' proposed here negates in turn transport of proteins, and asserts (i) local synthesis of axoplasmic proteins, corner stone of this model, (ii) existence of internal programs in axon and terminals, (iii) external control of programs resulting in local regulation of phenotype of axons and terminals, hence they are autonomous from perikarya; (iv) participation of perikarya through the fast transport in setting up the axonal programs but not in their immediate regulation. The word merotrophism...
Tipo: Journal article
Palavras-chave: Plasticity; Regeneration; Schwann cell; Slow transport; Sprouting; Trophism; Wld s.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200014

Digestive Phenotypic Flexibility in Post-Metamorphic Amphibians: Studies on a Model Organism
Provedor de dados: 48
Autores: NAYA,DANIEL E; BOZINOVIC,FRANCISCO.
Studies of phenotypic flexibility are central to the understanding of evolutionary and comparative physiology. Research conducted on many vertebrate species has shown that the digestive system is highly responsive and sensitive to environmental cues. However, amphibians, which are a standard and classic model organism for the study of many physiological processes, have been poorly considered in the study of ecological consequences on digestive flexibility. Here we review and analyze the current information on this topic for amphibians. We identify three major bodies of empirical evidence: a) seasonal changes in gut development, b) lack of dietary modulation of gut attributes in adult individuals, c) a relationship between feeding habits and the magnitude...
Tipo: Journal article
Palavras-chave: Amphibians; Digestive theory; Gut adjustments; Phenotypic flexibility.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300002

Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers
Provedor de dados: 48
Autores: SCHNEIDER,MARTIN F; RODNEY,GEORGE G.
Local discrete elevations in myoplasmic Ca2+ (Ca2+ sparks) arise from the opening of a small group of RyRs. Summation of a large number of Ca2+ sparks gives rise to the whole cell Ca2+ transient necessary for muscle contraction. Unlike sarcoplasmic reticulum vesicle preparations and isolated single channels in artificial membranes, the study of Ca2+ sparks provides a means to understand the regulation of a small group of RyRs in the environment of a functionally intact triad and in the presence of endogenous regulatory proteins. To gain insight into the mechanisms that regulate the gating of RyRs we have utilized laser scanning confocal microscopy to measure Ca2+ sparks in permeabilized frog skeletal muscle fibers. This review summarizes our recent studies...
Tipo: Journal article
Palavras-chave: Excitation-Contraction Coupling; Ca2+ Sparks; IperatoxinA; DP4; Calmodulin.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400016

Reduced oxygen diffusion across the shell of Gray gull (Larus modestus) eggs
Provedor de dados: 48
Autores: MONGE,C C; OSTOJIC,H; AGUILAR,R; CIFUENTES,V.
Gray gulls, Larus modestus, nest 1500 m above sea level in northern Chile's Atacama Desert, one of the driest in the world. Their eggshell gas permeability, one third of that found in other Larus species, is an adaptation that reduces water loss, but at the expense of oxygen diffusion into the air cell with resultant hypoxia and reduced metabolic rate. This contrasts with characteristics found in birds nesting at very high altitudes where oxygen diffusion across the egg shell is maximized at the expense of water conservation. The oxygen consumption (MO2) of Larus modestus is 66% that of Larus argentatus; the oxygen conductance (GO2) is equivalent to 48% of that obtained in 5 other bird species. The oxygen partial pressure (PAO2) in the air chamber of Larus...
Tipo: Journal article
Palavras-chave: Atacama; Eggshell; Oxygen conductance; Oxygen consumption; Gray gull (Larus modestus).
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300007

Antagonism of morphine-induced central respiratory depression by donepezil in the anesthetized rabbit
Provedor de dados: 48
Autores: TSUJITA,MIKI; SAKURABA,SHIGEKI; KURIBAYASHI,JUNYA; HOSOKAWA,YUKI; HATORI,EIKI; OKADA,YASUMASA; KASHIWAGI,MASANORI; TAKEDA,JUNZO; KUWANA,SHUN-ICHI.
Morphine is often used in cancer pain and postoperative analgesic management but induces respiratory depression. Therefore, there is an ongoing search for drug candidates that can antagonize morphine-induced respiratory depression but have no effect on morphine-induced analgesia. Acetylcholine is an excitatory neurotransmitter in central respiratory control and physostigmine antagonizes morphine-induced respiratory depression. However, physostigmine has not been applied in clinical practice because it has a short action time, among other characteristics. We therefore asked whether donepezil (a long-acting acetylcholinesterase inhibitor used in the treatment of Alzheimer's disease) can antagonize morphine-induced respiratory depression. Using the...
Tipo: Journal article
Palavras-chave: Morphine; Donepezil; Rabbit; Respiratory depression; Alzheimer's disease; Cholinesterase inhibitor.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400008

Metabolic interactions between hyperhomocysteinemia and endothelin-1 among Tunisian patients with acute coronary diseases
Provedor de dados: 48
Autores: Chalghoum,Abdelkader; Noichri,Yosri; Karkouch,Ines; Dandana,Azza; Baudin,Bruno; Jeridi,Guieder; Ferchichi,Salima; Miled,Abdelhédi.
BACKGROUND: Acute coronary syndromes (ACS) are complex and polygenic diseases which are a real problem of public health. These syndromes require multidisciplinary studies to understand the pathogenesis mechanisms and metabolic interactions between different risk factors.This study aimed to explore the variation of two coronary risk parameters not mentioned by Framingham cohorts, hyperhomocysteinemia and endothelin-1 (ET-1) in Tunisian coronary and the study of the variation of these parameters based on various cardiac risk factors and metabolic relationship between them.To 157 coronary and 142 healthy subjects, the concentration of homocysteine was quantified by fluorescence polarization immunoassay; the concentration of ET-1 was measured by an analytical...
Tipo: Journal article
Palavras-chave: Acute coronary syndrome; Endothelin-1; Hyperhomocysteinemia; Risk factors.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100032

Rat dorsal prostate is necessary for vaginal adhesion of the seminal plug and sperm motility in the uterine horns
Provedor de dados: 48
Autores: Tlachi-López,José L; López,Aurora; Hoffman,Kurt; Velázquez-Moctezuma,Javier; García-Lorenzana,Mario; Lucio,Rosa Angélica.
The rat prostate comprises dorsal, ventral and lateral lobes that are morphologically and biochemically distinct. Lesions to these structures are expected to affect the quality of the ejaculate and male fertility. In experiment 1, we analyzed ejaculate parameters of males that had chemical lesions of the dorsal or ventral lobes. At pre-lesion and at 5 and 20 days post-lesion males were mated, and after ejaculation, seminal fluid and seminal plug were obtained from the mated females. In experiment 2, the ventral lobes were ablated, and the ejaculate was analyzed. In experiment 3, the fertility of males with chemically-lesioned dorsal lobes or ablation of the ventral lobes was evaluated. Chemical lesion of the dorsal lobe prevented the adhesion of the...
Tipo: Journal article
Palavras-chave: Ejaculation; Characteristics of semen; Copulatory plug; Prostate; Seminal analysis; Sperm number.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300007

Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
Provedor de dados: 48
Autores: Zivkovic,Irena P; Stojanovic,Marijana M; Petrusic,Vladimir Z; Inic-Kanada,Aleksandra B; Micic,Mileva V; Dimitrijevic,Ljiljana A.
We have already demonstrated (Stojanovic et al., 2009) a connection between tetanus toxoid (TTd) hyperimmunization and the induction of anti-phospholipid syndrome (APS) in BALB/c mice. Here we show that C57BL/6 mice subjected to an identical procedure do not exhibit any like pathology attributable to anti-phospholipid antibodies; we explain that this absence results from idiotypic connectivity. Six groups of C57BL/6 mice were hyperimmunized with TTd in aluminum hydroxide or glycerol, with or without pretreatments. Pretreated mice had been injected with polyclonal or nonspecific immune stimulators, such as complete Freund's adjuvant (CFA) or glycerol. The epitope specificity of induced antibodies was tested by indirect ELISA using a tetanus toxoid immunogen...
Tipo: Journal article
Palavras-chave: Autoantibodies; C57BL/6 mice; Hyperimmunization; Network.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400003

Superoxide dismutase and catalase: tissue activities and relation with age in the long-lived species Margaritifera margaritifera
Provedor de dados: 48
Autores: FERNÁNDEZ,CARLOS; SAN MIGUEL,EDUARDO; FERNÁNDEZ-BRIERA,ALMUDENA.
Free radicals are extremely reactive and produce damage and modify cell functions. Furthermore, superoxide dismutase and catalase are believed to play a key role in the enzymatic defence of the cells. Indeed, some authors have argued that reduced free-radical damage could explain increased longevity. Margaritifera margaritifera is one of the longest-lived animals in the world (up to 100-200 years). Furthermore, this organism may serve as a useful model for gerontologists interested in exploring the mechanisms that promote long life and the slowing of senescence. The present study estimated for the first time individual enzymatic activity for superoxide dismutase isozymes (Cu,Zn-SOD and Mn-SOD) and catalase in tissue preparations of gills, digestive glands...
Tipo: Journal article
Palavras-chave: Margaritifera margaritifera; Free radicals; Aging; Superoxide dismutase; Catalase.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100006

Human erythroid progenitors from adult bone marrow and cord blood in optimized liquid culture systems respectively maintained adult and neonatal characteristics of globin gene expression
Provedor de dados: 48
Autores: ZHANG,XIN; MA,YAN-NI; ZHANG,JUN-WU.
In vitro suspension culture procedures for erythroid progenitor cells make it possible for us to obtain large cultures of erythrocyte populations for the investigation of globin gene switching. In this study we aimed to establish optimized culture systems for neonatal and adult erythroblasts and to explore the globin expression patterns in these culture systems. To culture CD34+ cells purified from human umbilical cord blood (CB) and adult bone marrow (BM), we respectively replaced the fetal bovine serum (FBS) with human cord serum and human adult serum. These CD34+ cells were then induced to erythroid differentiation. All the globin mRNA (including á-, &amp;#958;-, &amp;#946;-, &amp;#947;-and &amp;#949;-globin), the hemoglobin (Hb)-producing erythroid...
Tipo: Journal article
Palavras-chave: Adult bone marrow; Cord blood; Erythroid progenitor; Globins; Liquid culture.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100005

Functional disruption of human leukocyte antigen II in human embryonic stem cell
Provedor de dados: 48
Autores: Chen,Haide; Li,Yang; Lin,Xijuan; Cui,Di; Cui,Chun; Li,Hui; Xiao,Lei.
BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact same type of cells derived from hESCs. Allo-graft immune rejection is one of the obstacles for hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to CD4+ T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification RESULTS: Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator (CIITA) in hESCs efficiently. CIITA-/-hESCs did...
Tipo: Journal article
Palavras-chave: HESCs; CIITA; TALENs; Immune rejection.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100059

Adult mesenchymal stem cell therapy for myelin repair in Multiple Sclerosis
Provedor de dados: 48
Autores: Rivera,Francisco J; Aigner,Ludwig.
Multiple sclerosis (MS) is a demyelinating immune-mediated disease of the central nervous system (CNS). It is the most frequent neurological disease in young adults and affects over 2 million people worldwide. Current treatments reduce the relapse rate and the formation of inflammatory lesions in the CNS, but with only temporary and limited success. Despite the presence of endogenous oligodendroglial progenitors (OPCs) and of spontaneous remyelination, at least in early MS its levels and its qualities are apparently insufficient for a sustained endogenous functional repair. Therefore, novel MS therapies should consider not only immunemodulatory but also myelin repair activities. Mesenchymal stem cells (MSCs) represent an attractive alternative to develop a...
Tipo: Journal article
Palavras-chave: Multiple sclerosis; Remyelination; Oligodendrocyte progenitor cells; Mesenchymal stem cell therapy and functional recovery.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300007

Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes
Provedor de dados: 48
Autores: CARRILLO,JUAN C; OJEDA,VERÓNICA A; CAMPOS-DE QUIROZ,HUGO A; ORTEGA,FERNANDO M.
A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our findings showed that stem meristems were easier to obtain, presented lower levels of contamination and a better development than crown meristems. The L2 medium showed better results than B5 and MS media for the cultivars and experimental lines studied. L2 medium supplemented with 0.003 mg/l of 4-amino-3,5,6-trichloropicolinic acid and 1.0 mg/l of 6-benzylaminopurine gave consistently better results and will be applied in our breeding program to propagate,...
Tipo: Journal article
Palavras-chave: In vitro culture; Meristem culture; Red clover; Trifolium pratense L.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100005

Cloning, molecular characterization and expression of a cDNA encoding a functional NADH-cytochrome b5 reductase from Mucor racemosus PTCC 5305 in E. coli
Provedor de dados: 48
Autores: SETAYESH,NED A; SEPEHRIZADEH,ZARGHAM; JABERI,ELHAM; YAZDI,MOJTABA TABATABAEI.
The present work aims to study a new NADH-cytochrome b5 reductase (cb5r) from Mucor racemosus PTCC 5305. A cDNA coding for cb s r was isolated from a Mucor racemosus PTCC 5305 cDNA library. The nucleotide sequence of the cDNA including coding and sequences flanking regions was determined. The open reading frame starting from ATG and ending with TAG stop codon encoded 228 amino acids and displayed the closest similarity (73%) with Mortierella alpina cb s r. Lack of hydrophobic residues in the N-terminal sequence was apparent, suggesting that the enzyme is a soluble isoform. The coding sequence was then cloned in the pET16b transcription vector carrying an N-terminal-linked His-Tag® sequence and expressed in Escherichia coli BL21 (DE3). The enzyme was then...
Tipo: Journal article
Palavras-chave: Mucor racemosus; NADH-cytochrome b s reductase; Soluble isoform.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200001

Transcriptome responses to heat- and cold-stress in ladybirds (Cryptolaemus montrouzieri Mulasnt) analyzed by deep-sequencing
Provedor de dados: 48
Autores: Zhang,Yuhong; Wu,Hongsheng; Xie,Jiaqin; Jiang,Ruixin; Deng,Congshuang; Pang,Hong.
BACKGROUND: Changed temperature not only threaten agricultural production, but they also affect individual biological behavior, population and community of many insects, and consequently reduce the stability of our ecosystem. Insect's ability to respond to temperature stress evolved through a complex adaptive process, thus resulting in varied temperature tolerance among different insects. Both high and low extreme temperatures are detrimental to insect development since they constitute an important abiotic stress capable of inducing abnormal biological responses. Many studies on heat or cold tolerance of ladybirds have focused on measurements of physiological and biochemical indexes such as supercooling point, higher/lower lethal temperatures, survival...
Tipo: Journal article
Palavras-chave: Coccinellidae; Heat tolerance; Cold tolerance; Digital gene expression.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100066

Cyanobacterial defense mechanisms against foreign DNA transfer and their impact on genetic engineering
Provedor de dados: 48
Autores: Stucken,Karina; Koch,Robin; Dagan,Tal.
Cyanobacteria display a large diversity of cellular forms ranging from unicellular to complex multicellular filaments or aggregates. Species in the group present a wide range of metabolic characteristics including the fixation of atmospheric nitrogen, resistance to extreme environments, production of hydrogen, secondary metabolites and exopolysaccharides. These characteristics led to the growing interest in cyanobacteria across the fields of ecology, evolution, cell biology and biotechnology. The number of available cyanobacterial genome sequences has increased considerably in recent years, with more than 140 fully sequenced genomes to date. Genetic engineering of cyanobacteria is widely applied to the model unicellular strains Synechocystis sp. PCC 6803...
Tipo: Journal article
Palavras-chave: Cyanobacteria; Genetic engineering; Defense mechanism; Restriction-modification.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400009

Internucleotide correlations and nucleotide periodicity in Drosophila mtDNA: New evidence for panselective evolution
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
Analysis for the homogeneity of the distribution of the second base of dinucleotides in relation to the first, whose bases are separated by 0, 1, 2,... 21 nucleotide sites, was performed with the VIH-1 genome (cDNA), the Drosophila mtDNA, the Drosophila Torso gene and the human p-globin gene. These four DNA segments showed highly significant heterogeneities of base distributions that cannot be accounted for by neutral or nearly neutral evolution or by the "neighbor influence" of nucleotides on mutation rates. High correlations are found in the bases of dinucleotides separated by 0, 1 and more number of sites. A periodicity of three consecutive significance values (measured by the x²9) was found only in Drosophila mtDNA. This periodicity may be due to an...
Tipo: Journal article
Palavras-chave: Nucleotide heterogeneity; DNA periodicity; Dinucleotide analysis; Neutral evolution; Panselective evolution.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400014

Calmodulin and Calcium-release Channels
Provedor de dados: 48
Autores: KASRI,NAEL NADIF; PARYS,JAN B.; CALLEWAERT,GEERT; MISSIAEN,LUDWIG; DE SMEDT,HUMBERT.
Calmodulin (CaM) is a ubiquitous cytosolic protein that plays a critical role in regulating cellular functions by altering the activity of a large number of ion channels. There are many examples for CaM directly mediating the feedback effects of Ca2+ on Ca2+ channels. Recently the molecular mechanisms by which CaM interacts with voltage-gated Ca2+ channels, Ca2+-activated K+ channels and ryanodine receptors have been clarified. CaM plays an important role in regulating these ion channels through lobe-specific Ca2+ detection. CaM seems to behave as a channel subunit. It binds at low [Ca2+] and undergoes conformational changes upon binding of Ca2+, leading to an interaction with another part of the channel to regulate its gating. Here we focus on the...
Tipo: Journal article
Palavras-chave: Calmodulin calcium channels inositol 1; 4; 5-trisphosphate receptor.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400011

Chronic toxicity bioassay with populations of the crustacean Artemia salina exposed to the organophosphate diazinon
Provedor de dados: 48
Autores: Bustos-Obregon,Eduardo; Vargas,Álvaro.
A chronic toxicity bioassay was conducted with the microcrustacean Artemia salina as the testing organism for the toxic organophosphate diazinon in order to determine if the species is an appropriate indicator of pollution in aquatic environments. Tests of animal exposure to different concentrations of the toxicant were performed for 24, 48 and 72 hours after larvae hatching. Registered mortality data was used to obtain the lethal dose 50 (LD50) of the organophosphate for each exposure time, considering the immobilization of A. salina larvae as the mortality parameter. The lethal concentration (LD50) in the same exposure times was calculated by evaluating morphological changes on the three initial stages of larval development. Both doses were determined by...
Tipo: Journal article
Palavras-chave: Diazinon; Artemia salina; Ecotoxicology; Bioassay.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300013

Endoplasmic reticulum calcium signaling in nerve cells
Provedor de dados: 48
Autores: VERKHRATSKY,ALEXEI.
The endoplasmic reticulum (ER) is an important organelle involved in various types of signaling in nerve cells. The ER serves as a dynamic Ca2+ pool being thus involved in rapid signaling events associated with cell stimulation by either electrical (action potential) or chemical (neurotransmitters) signals. This function is supported by Ca2+ release channels (InsP3 and ryanodine receptors) and SERCA Ca2+ pumps residing in the endomembrane. In addition the ER provides a specific environment for the posttranslational protein processing and transport of various molecules towards their final destination. In parallel, the ER acts as a "calcium tunnel," which facilitates Ca2+ movements within the cell by avoiding cytoplasmic routes. Finally the ER appears as a...
Tipo: Journal article
Palavras-chave: Calcium signaling; Endoplasmic reticulum; Ryanodine receptors; InsP3 receptors.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400027

Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and Go/G1 phase arrest in human leukemia HL-60 cells
Provedor de dados: 48
Autores: Pieme,Constant Anatole; Santosh,Guru Kumar; Tekwu,Emmanuel Mouafo; Askun,Tülin; Aydeniz,Hatice; Ngogang,Jeanne Yonkeu; Bhushan,Shashi; Saxena,Ajit Kumar.
BACKGROUND: Zanthoxylum heitzii is a spice used to prepare several dishes and to treat tumors, syphilis, malaria, cardiac palpitations, urogenital infections in the west region of Cameroon, but the antitumor mechanisms and chemical composition are not yet investigated. This study was aimed to determine the antiproliferative effects of four extracts from the fruits and barks of Zanthoxyllum heitzii (Rutaceae) on apoptosis in human promyelocytic cells, their mechanisms and the chemical composition. The 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the fifty percent inhibition (IC50) concentration of the cell lines after treatment. The effect on morphology was observed using a light or fluorescence...
Tipo: Journal article
Palavras-chave: Zanthozyllum heitzii; Syringic acid; Apoptosis; HL-60 cells; ROS; Mitochondrial membrane potential.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100048

Comparative acute toxicity of gallium(III), antimony(III), indium(III), cadmium(II), and copper(II) on freshwater swamp shrimp (Macrobrachium nipponense)
Provedor de dados: 48
Autores: Yang,Jen-Lee.
BACKGROUND: Acute toxicity testing were carried out the freshwater swamp shrimp, Macrobrachium nipponense, as the model animal for the semiconductor applied metals (gallium, antimony, indium, cadmium, and copper) to evaluate if the species is an suitable experimental animal of pollution in aquatic ecosystem. RESULTS: The static renewal test method of acute lethal concentrations determination was used, and water temperature was maintained at 24.0&amp;#8201;±&amp;#8201;0.5°C. Data of individual metal obtained from acute toxicity tests were determined using probit analysis method. The median lethal concentration (96-h LC50) of gallium, antimony, indium, cadmium, and copper for M. nipponense were estimated as 2.7742, 1.9626, 6.8938, 0.0539, and 0.0313 mg/L,...
Tipo: Journal article
Palavras-chave: Macroburachium nipponense; Cu; Cd; Sb; Ga; In; LC50.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100013

MnSOD and GPx1 polymorphism relationship with coronary heart disease risk and severity
Provedor de dados: 48
Autores: Souiden,Yosra; Mallouli,Hela; Meskhi,Salah; Chaabouni,Yassine; Rebai,Ahmed; Chéour,Foued; Mahdouani,Kacem.
BACKGROUND: Disturbance of the equilibrium between reactive oxygen species (ROS) and anti-oxidants (AOX) has been implicated in various diseases, including atherosclerosis, the most common pathologic process underlying coronary heart disease (CHD). Thus, the defense systems against ROS are critical protecting blood vessel walls against oxidative damage. In this study, we investigate whether Ala16Val MnSOD and Pro198Leu GPx polymorphisms are associated with CHD susceptibility and/or severity METHODS: Both polymorphisms were genotyped in a sample of 203 controls and 164 patients. CHD risk and severity, antioxidant status (enzymatic and/or non enzymatic) and biochemical parameters were assessed and analysed by genotype RESULTS: A significant association of...
Tipo: Journal article
Palavras-chave: Genetic polymorphism; Coronary heart disease; SOD activity; GPx activity; Total antioxidant status; Atherosclerosis.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100022

A possible role of Drosophila CTCF in mitotic bookmarking and maintaining chromatin domains during the cell cycle
Provedor de dados: 48
Autores: Shen,Wenlong; Wang,Dong; Ye,Bingyu; Shi,Minglei; Zhang,Yan; Zhao,Zhihu.
BACKGROUND: The CCCTC-binding factor (CTCF) is a highly conserved insulator protein that plays various roles in many cellular processes. CTCF is one of the main architecture proteins in higher eukaryotes, and in combination with other architecture proteins and regulators, also shapes the three-dimensional organization of a genome. Experiments show CTCF partially remains associated with chromatin during mitosis. However, the role of CTCF in the maintenance and propagation of genome architectures throughout the cell cycle remains elusive. RESULTS: We performed a comprehensive bioinformatics analysis on public datasets of Drosophila CTCF (dCTCF). We characterized dCTCF-binding sites according to their occupancy status during the cell cycle, and identified...
Tipo: Journal article
Palavras-chave: DCTCF; Cell cycle; Chromatin domains; Mitotic bookmarking; Bioinformatics.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100027

Gastroprotective effect of the ethanolic extract of Parkia platycephala Benth. leaves against acute gastric lesion models in rodents
Provedor de dados: 48
Autores: Fernandes,Hélio B; Silva,Francilene V; Passos,Flávia Franceli B; Bezerra,Roosevelt D S; Chaves,Mariana H; Oliveira,Francisco A; Meneses Oliveira,Rita C.
Parkia platycephala Benth. (Leguminosae - Mimosoideae), popularly known as "visgueira", fava bean tree or "fava-de-bolota", is widely found in the Northern and Northeastern regions of Brazil. Its pods are used as cattle food supplement in the drought period. Compounds with a gastroprotective activity were obtained from the genus Parkia. Therefore, this study aimed at investigating the gastroprotective effect of the ethanolic extract of Parkia platycephala Benth. leaves (Pp-EtOH), as well as evaluating its possible mechanisms of action in experimental ulcer induction models. Lesions were induced by absolute ethanol, ethanol-HCl, ischemia-reperfusion and indomethacin in rodents. Pp-EtOH showed a protective effect in the lesion models (66, 48 and 52 %,...
Tipo: Journal article
Palavras-chave: Antioxidant; Ethanol; Gastroprotection; Nitric oxide; Parkia platycephala; Ulcer.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400010

Fe de Erratas
Provedor de dados: 48
Tipo: Journal article
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200015

Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation
Provedor de dados: 48
Autores: CHENG,SHU-QUN; HE,JUN-LIN; DONG,YAN-LING; LIU,XUE-QING; DING,YU-BIN; GAO,RU-FEI; TAN,YI; YE,QIAN; TIAN,ZHEN-LING; WANG,YING-XIONG.
Calreticulin (CRT), a Ca2+-binding storage protein and chaperone in the endoplasmic reticulum, modulates cell adhesiveness and integrin-dependent Ca2+ signaling. However, the role of CRT during implantation remains poorly understood. In the present study, we characterized the expression of CRT mRNA and the protein in mouse endometria from pregnancy DI to D7. Real-Time PCR and in situ hybridization results showed that the levels of CRT mRNA in the endometria of pregnant mice were significantly higher than those of non-pregnant mice (P&lt;0.05), and increased gradually from pregnancy DI to D4, reaching the máximum level on D4, followed by a plateau from D4 to D7. Using immunofluorescence histochemistry and western blot, changes of CRT expression in the...
Tipo: Journal article
Palavras-chave: Calreticulin; Endometrium; Embryo implantation; Mouse.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400012

The comparative mitogenomics and phylogenetics of the two grouse-grasshoppers (Insecta, Orthoptera, Tetrigoidea)
Provedor de dados: 48
Autores: Sun,Yufang; Liu,Dianfeng; Xiao,Bo; Jiang,Guofang.
Abstract  Objective  This study aimed to reveal the mitochondrial genomes (mtgenomes) of Tetrix japonica and Alulatettix yunnanensis, and the phylogenetics of Orthoptera species.  Methods  The mtgenomes of A. yunnanensis and T. japonica were firstly sequenced and assembled through partial sequences amplification, and then the genome organization and gene arrangement were analyzed. Based on nucleotide/amino acid sequences of 13 protein-coding genes and whole mtgenomes, phylogenetic trees were established on 37 Orthoptera species and 5 outgroups, respectively.  Results  Except for a regulation region (A+T rich region), a total of 37 genes were found in mtgenomes of T. japonicaand A. yunnanensis, including 13 protein-coding genes, 2 ribosomal RNA genes, and...
Tipo: Journal article
Palavras-chave: Mitogenome; Orthoptera Tetrigoidea; Phylogenetic.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100219

XXXIX REUNIÓN ANUAL DE GENÉTICA DE CHILE
Provedor de dados: 48
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000300008

XLVII Reunión Anual de la Sociedad de Biología de Chile
Provedor de dados: 48
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500009

Expression of non-neuronal cholinergic system in maxilla of rat in vivo
Provedor de dados: 48
Autores: Guo,Jie; Wang,Lue; Xu,Haihua; Che,Xiaoxia.
BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits &amp;#945;1, &amp;#945;2, &amp;#945;3, &amp;#945;5,...
Tipo: Journal article
Palavras-chave: Non-neuronal cholinergic system; Maxilla; Real-Time PCR; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100066

The ingestión of a Nectandra membranácea extract changes the bioavailability of technetium-99m radiobiocomplex in rat organs
Provedor de dados: 48
Autores: RAMOS FARÍAS MORENO,SILVANA; ARNOBIO,ADRIANO; CARVALH0,JORGE JOSÉ DE; NASCIMENT0,LUCIA; OLIVEIRA TIMOTEO,MARGARETH; OLEJ,BENI; KAZAN ROCHA,EMELY; PEREIRA,MARIO; BERNARDO-FILH0,MARIO; QUERINO DE ARAÚJO CALDAS,LUIZ.
The radiobiocomplexes labeled with technetium-99m (Tc-99m) have been widely used in nuclear medicine in single photon emission computed tomography and in basic research. The aim of this study was to assess the influence of a Nectandra membranácea extract on the bioavailability of the sodium pertechnetate (Na99mTc0(4)) radiobiocomplex in rat organs. The animals were treated with a N. membranácea extract (30 mg/ ml), for 6 days. Na99mTc0(4) was injected, the organs were isolated and weighed, and the radioactivity was determined in each organ (%ATI/organ). The %ATI/organ was divided by the mass of each organ to calculate the %ATI/g. A significant increase of the %ATI/organ of Na99mTc0(4) was observed in muscle and thyroid as well as in the %ATI/g in the...
Tipo: Journal article
Palavras-chave: Nectandra membranácea; Bioavailability; Sodium pertechnetate; Radiobiocomplex.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200004

Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions
Provedor de dados: 48
Autores: CIFUENTES,ANA S; GONZÁLEZ,MARIELA A; VARGAS,SILVIA; HOENEISEN,MARITZA; GONZÁLEZ,NELSON.
The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was carried out under autotrophic (with NaNO3, NH4Cl and urea) and mixotrophic conditions (with 4, 8, 12 mM sodium acetate) under two photon flux densities (PFD) (35 and 85 µmol m-2 s-1). The carotenogenesis was induced by 1) addition of NaCl (0.2 and 0.8 %), 2) N-deprivation and 3) high PFD (150 µmol m-2 s-1). Total carotenoids were estimated by spectrophotometry and total astaxanthin by HPLC. Ammonium chloride was the best N-source for growth (k=0.7 div day-1,...
Tipo: Journal article
Palavras-chave: Astaxanthin; Carotenogenesis; Growth; Haematococcus pluvialis.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300006

Impact of the Training Program on Lipid Profile and Cardiac Health
Provedor de dados: 48
Autores: BURNEIKO,REGINA COELI; DINIZ,YEDA SANT'ANA; FAINE,LUCIANE APARECIDA; MACHADO GALHARDI,CRISTIANO; PADOVANI,CARLOS ROBERTO; NOVELLI,ETHEL LOURENZI B.; CICOGNA,ANTONIO CARLOS.
The aim of this study was to investigate the effects of training programs on serum lipid profile and myocardial oxidative stress. Male Wistar rats (2 mo-old) were divided into three groups (n=8): sedentary (S), loadless trained (T) and trained-overload 2% body weight (TL). T and TL were trained through swimming for 9 weeks. T and TL rats had increased myocardial lipoperoxide (TBA) and lipid hydroperoxide (HP), whereas HP was higher in TL than in T animals. Superoxide dismutase (SOD) activities were lowest in TL. Myocardial glutathione peroxidase (GSH-Px) was lower in TL than in T and S rats. TL decreased HDL-cholesterol and increased LDL-cholesterol. The serum lactate dehydrogenase and TBA were increased, while SOD and GSH-Px activities were decreased in...
Tipo: Journal article
Palavras-chave: Training; Lipid profile; Oxidative stress; Heart; Serum.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100006

Genetic and cytological characterization of a developmental mutant of Aspergillus nidulans induced by 5-azacytidine
Provedor de dados: 48
Autores: COSTA,MARCO ANTONIO; SILVA,NELMARA CRISTINA GONÇALVES; CASTRO-PRADO,MARIALBA AVEZUM ALVES.
An analysis of a new medusa mutant of Aspergillus nidulans obtained by 5-azacytidine-treatment and named B116 is provided. The B116 mutant was phenotypically characterized by the production of conidiophores with reduced pigmentation and vesicles bearing multiple tiers of sterigmata. A single nuclear gene located on chromosome I is responsible for phenotypical changes in the mutant. The 5-azacytidine-altered locus, designated medA102, is recessive in heterozygous diploid and the medusa mutant is a Dp(II,I) duplication bearer that renders the strain mitotically unstable.
Tipo: Journal article
Palavras-chave: Conidiation; Developmental program; Medusa.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200012

Structural biology of chemokine receptors
Provedor de dados: 48
Autores: ROJO,DANIEL; SUETOMI,KATSUTOSHI; NAVARRO,JAVIER.
Chemokine receptors are G protein-coupled receptors that mediate migration and activation of leukocytes as an important part of a protective immune response to injury and infection. In addition, chemokine receptors are used by HIV-1 to infect CD4 positive cells. The structural bases of chemokine receptor recognition and signal transduction are currently being investigated. High-resolution X-ray diffraction and NMR spectroscopy of chemokines indicate that all these peptides exhibit a common folding pattern, in spite of its low degree of primary-sequence homology. Chemokines' functional motifs have been identified by mutagenesis studies, and a possible mechanism for receptor recognition and activation is proposed, but high-resolution structure data of...
Tipo: Journal article
Palavras-chave: Chemokines; G-protein coupled receptors; Inflammation; HIV-1.
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400006

Jaime Alvarez and the case against slow axoplasmic transport: some epistemological reflections
Provedor de dados: 48
Autores: SERANI-MERLO,ALEJANDRO.
The 'slow axoplasmic transport theory' has been the prevailing view over the last forty years in order to explain the metabolic maintenance of neuronal axons and nerve endings. A significant amount of evidence against this theoretic interpretation of the existing experimental data has been presented by J. Alvarez, A. Giuditta and E. Koenig in an exhaustive review. They propose an alternative theoretical interpretation called the 'local synthesis model', integrating recent evidence for axon biology and regeneration. We present some epistemological considerations that reinforce the above criticisms and propositions.
Tipo: Journal article
Palavras-chave: Axon biology; Biological epistemology; Neuron; Slow axonal transport.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200015

Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia
Provedor de dados: 48
Autores: Paudel,Babita; Bhattarai,Hari Datta; Chan Kim,Il; Lee,Hyoungseok; Sofronov,Roman; Ivanova,Lena; Poryadina,Lena; Yim,Joung Han.
BACKGROUND: Several plants are reported to be produced various biological active compounds. Lichens from the extreme environments such as high altitude, high UV, drought and cold are believed to be synthesized unique types of secondary metabolites than the other one. Several human pathogenic bacteria and fungi have been muted into drug resistant strains. Various synthetic antioxidant compounds have posed carcinogenic effects. This phenomenon needs further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biological active compounds from plants of subarctic origin. RESULTS: A total of 114 plant species, including 80 species of higher plants, 19 species of lichens and 15 species of mosses, were collected from...
Tipo: Journal article
Palavras-chave: Antimicrobial; Antioxidant; Brine shrimp; DPPH; Lichen; Moss.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100010

Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis
Provedor de dados: 48
Autores: Ortiz-Severín,Javiera; Varas,Macarena; Bravo-Toncio,Catalina; Guiliani,Nicolás; Chávez,Francisco P.
BACKGROUND: Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence FINDINGS: By using Phenotypic Microarrays (PM) we analyzed near 2000 phenotypes of P. aeruginosa PAO1 polyP kinase mutants (ppk1 and ppk2). We found that both ppk mutants shared most of the phenotypic changes and interestingly many of them related to susceptibility toward numerous and different type of antibiotics such as Ciprofloxacin, Chloramphenicol and Rifampicin CONCLUSIONS: Combining the fact that ppk1 mutants have reduced virulence and are more susceptible to antibiotics, polyP synthesis and particularly PPK1, is a...
Tipo: Journal article
Palavras-chave: Antibiotic susceptibility; Polyphosphate kinase; Pseudomonas; Phenome; Phenotypic microarrays; Multidrug resistance.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100022

Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos
Provedor de dados: 48
Autores: Arias,María E; Ross,Pablo J; Felmer,Ricardo N.
Different culture systems have been studied that support development of somatic cell nuclear transfer (SCNT) embryos up to the blastocyst stage. However, the use of sequential and two-step culture systems has been less studied. The objective of the present study was to examine the developmental potential and quality of bovine SCNT embryos cultured in different two-step culture media based on KSOM, SOF and the macromolecules FBS and BSA (K-K/FBS, K-S/BSA and K-K/BSA, respectively). No differences were observed in the cleavage rate for any of the culture systems. However, there was a significant difference (P&lt;0.01) in the rate of blastocyst development, with the K-K/ FBS culture system yielding a higher rate of blastocysts (28%) compared to other...
Tipo: Journal article
Palavras-chave: Cattle; Gene expression; KSOM; Nuclear transfer embryos; SOF.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400016

Selective intra-dinucleotide interactions and periodicities of bases separated by K sites: a new vision and tool for phylogeny analyses
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
Abstract Direct tests of the random or non-random distribution of nucleotides on genomes have been devised to test the hypothesis of neutral, nearly-neutral or selective evolution. These tests are based on the direct base distribution and are independent of the functional (coding or non-coding) or structural (repeated or unique sequences) properties of the DNA. The first approach described the longitudinal distribution of bases in tandem repeats under the Bose&amp;#8211;Einstein statistics. A huge deviation from randomness was found. A second approach was the study of the base distribution within dinucleotides whose bases were separated by 0, 1, 2&amp;#8230; K nucleotides. Again an enormous difference from the random distribution was found with...
Tipo: Journal article
Palavras-chave: Evolutionary theories; Selective nucleotide interactions; Selective periodicities.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100501

Model of chromosome associations in Mus domesticus spermatocytes
Provedor de dados: 48
Autores: Berríos,Soledad; Manterola,Marcia; Prieto,Zulita; López-Fenner,Julio; Page,Jesús; Fernández-Donoso,Raúl.
Understanding the spatial organization of the chromosomes in meiotic nuclei is crucial to our knowledge of the genome's functional regulation, stability and evolution. This study examined the nuclear architecture of Mus domesticus 2n=40 pachytene spermatocytes, analyzing the associations among autosomal bivalents via their Centromere Telomere Complexes (CTC). The study developed a nuclear model in which each CTC was represented as a 3D computer object. The probability of a given combination of associations among CTC was estimated by simulating a random distribution of 19 indistinguishable CTC over n indistinguishable "cells" on the nuclear envelope. The estimated association frequencies resulting from this numerical approach were similar to those obtained...
Tipo: Journal article
Palavras-chave: Bivalent associations; Nuclear model; Mouse spermatocytes; Heterochromatin distribution.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300003

Synthetic nanoemulsion resembling a protein-free model of 7-ketocholesterol containing low density lipoprotein: In vitro and in vivo studies
Provedor de dados: 48
Autores: Favero,Giovani M; Maranhão,Raul C; Maria,Durvanei A; Levy,Débora; Bydlowski,Sérgio P.
7-ketocholesterol (7-KC) differs from cholesterol by a functional ketone group at C7. It is an oxygenated cholesterol derivative (oxysterol), commonly present in oxidized low-density lipoprotein (LDL). Oxysterols are generated and participate in several physiologic and pathophysiologic processes. For instance, the cytotoxic effects of oxidized LDL have been widely attributed to bioactive compounds like oxysterols. The toxicity is in part due to 7-KC. Here we aimed to demonstrate the possibility of incorporating 7-KC into the synthetic nanoemulsion LDE, which resembles LDL in composition and behavior. This would provide a suitable artificial particle resembling LDL to study 7-KC metabolism. We were able to incorpórate 7-KC in several amounts into LDE. The...
Tipo: Journal article
Palavras-chave: Emulsion; LDL; LDE; 7-ketocholesterol; Oxidized LDL; Oxysterol.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400008

Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display
Provedor de dados: 48
Autores: SIQUIEROLI,ANA CAROLINA S; VIEIRA,CARLOS U; CARVALHO-ZILSE,GISLENE A; GOULART,LUIZ R; KERR,WARWICK E; BONETTI,ANA M.
In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA pro files of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The...
Tipo: Journal article
Palavras-chave: Determination of castes; Intercastes; Melipona sp.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100011

Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
Provedor de dados: 48
Autores: SÁNCHEZ,GITTITH; CUELLAR,DANIELA; ZULANTAY,INES; GAJARDO,MARTA; GONZÁLEZ-MARTIN,GUILLERMO.
The aim of the present study was to study the trypanocidal activity of nanoparticles loaded with nifurtimox in comparison with the free drug against Trypanosoma cruzi, responsible for Chagas' disease. Ethylcyanoacrylate nanoparticles acted as the delivery system into cells. As the obligate replicative intracellular form is amastigote, in vitro studies were performed on this form of parasite as well as on cell culture derived trypomastigotes. The fluorescence method used here was very useful as it allowed for the simultaneous study of trypanocide activity and cytotoxicity by determining living or dead parasites within living or dead host cells. According to these results, the greatest trypanocide activity on cell culture-derived trypomastigotes was recorded...
Tipo: Journal article
Palavras-chave: Nifurtimox; Nanoparticles; Amastigotes; Trypomastigotes; Trypanosoma cruzi.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100007

EDUARDO DEL SOLAR OSSES 1932 - 2001
Provedor de dados: 48
Autores: URETA,TITO.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200003

Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)
Provedor de dados: 48
Autores: Cid-Hernández,Margarita; Ramírez-Anguiano,Ana C; Ortiz,Genaro G; Morales-Sanchez,Eddic W; González-Ortiz,Luis J; Velasco-Ramírez,Sandra F; Pacheco-Moisés,Fermín P.
BACKGROUND: Karwinskia humboldtiana (Kh) is a poisonous plant of the rhamnacea family. To elucidate some of the subcellular effects of Kh toxicity, membrane fluidity and ATPase activities as hydrolytic and as proton-pumping activity were assessed in rat liver submitochondrial particles. Rats were randomly assigned into control non-treated group and groups that received 1,1.5 and 2 g/Kg body weight of dry powder of Kh fruit, respectively. Rats were euthanized at day 1 and 7 after treatment RESULTS: Rats under Kh treatment at all dose levels tested, does not developed any neurologic symptoms. However, we detected alterations in membrane fluidity and ATPase activity. Lower dose of Kh on day 1 after treatment induced higher mitochondrial membrane fluidity than...
Tipo: Journal article
Palavras-chave: Karwinskia humboldtiana; ATPase; Mitochondria; Membrane fluidity; PH gradient.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100017

Mitochondrial Function and Nitric Oxide Utilization
Provedor de dados: 48
Autores: CARRERAS,MARIA CECILIA; SCHÖPFER,FRANCISCO; LISDERO,CONSTANZA; RIOBÓ,NATALIA; PODEROSO,JUAN JOSÉ.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200018

Properties of realgar bioleaching using an extremely acidophilic bacterium and its antitumor mechanism as an anticancer agent
Provedor de dados: 48
Autores: Chen,Peng; Xu,Ruixiang; Yan,Lei; Wu,Zhengrong; Wei,Yan; Zhao,Wenbin; Wang,Xin; Xie,Qinjian; Li,Hongyu.
Abstract Realgar is a naturally occurring arsenic sulfide (or Xionghuang, in Chinese). It contains over 90% tetra-arsenic tetrasulfide (As4S4). Currently, realgar has been confirmed the antitumor activities, both in vitro and in vivo, of realgar extracted using Acidithiobacillus ferrooxidans (A. ferrooxidans). Bioleaching, a new technology to greatly improve the use rate of arsenic extraction from realgar using bacteria, is a novel methodology that addressed a limitation of the traditional method for realgar preparation. The present systematic review reports on the research progress in realgar bioleaching and its antitumor mechanism as an anticancer agent. A total of 93 research articles that report on the biological activity of extracts from realgar using...
Tipo: Journal article
Palavras-chave: A. ferrooxidans; Anticancer effect; Bioleaching; RBS; Realgar.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100502

Reply to Nespolo's paper entitled "New invariants and dimensionless numbers: Futile renaissance of old fallacies?"
Provedor de dados: 48
Autores: GÜNTHER,BRUNO; MORGADO,ENRIQUE.
Tipo: Journal article
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200002

In vivo and in vitro anti-inflammatory activity of Cryptostegia grandiflora Roxb. ex R. Br. leaves
Provedor de dados: 48
Autores: Castro,Jenny P; Ocampo,Yanet C; Franco,Luis A.
BACKGROUND: Despite Cryptostegia grandiflora Roxb. ex R. Br. (Apocynaceae) leaves are widely used in folk Caribbean Colombian medicine for their anti-inflammatory effects, there are no studies that support this traditional use. Therefore, this work aimed to evaluate the effect of the total extract and primary fractions obtained from Cryptostegia grandiflora leaves, using in vivo and in vitromodels of inflammation, and further get new insights on the mechanisms involved in this activity. RESULTS: Ethanolic extract of Cryptostegia grandiflora leaves, and its corresponding ether and dichloromethane fractions, significantly reduced inflammation and myeloperoxidase activity (MPO) in ear tissue of mice treated with 12-O-tetradecanoyl-phorbol-13-acetate (TPA)....
Tipo: Journal article
Palavras-chave: Ear edema; Free radicals; Inflammation; MPO; PGE2; RAW 264; 7 macrophages.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100032

Pituitary cell type-specific electrical activity, calcium signaling and secretion
Provedor de dados: 48
Autores: STOJILKOVIC,STANKO S.
All secretory anterior pituitary cells exhibit spontaneous and extracellular calcium-dependent electrical activity, but differ with respect to the patterns of firing and associated calcium signaling and hormone secretion. Thus, somatotrophs and lactotrophs fire plateau-bursting action potentials spontaneously and without coupling to calcium release from intracellular stores, which generate calcium signals of sufficient amplitude to keep steady hormone release. In these cells, both spontaneous electrical activity and basal hormone secretion can be further amplified by activation of Gq/11 and Gs-coupled receptors and inhibited by Gi/o/z-coupled receptors. In contrast, gonadotrophs fire single, high-amplitude spikes with limited ability to promote calcium...
Tipo: Journal article
Palavras-chave: Somatotrophs; Lactotrophs; Gonadotrophs; GH3 cells; SK channels; BK channels; Action potentials; Exocytosis.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300004

Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
Provedor de dados: 48
Autores: CUNHA,DANIEL A; AMARAL,MARIA EC; CARVALHO,CAROLINA PF; COLLARES-BUZATO,CARLA B; CARNEIRO,EVERARDO M; BOSCHERO,ANTONIO C.
During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic &amp;#946;-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17th-18th days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and...
Tipo: Journal article
Palavras-chave: Insulin; Pancreatic islets; Pregnant rats; Prolactin; SNARE proteins; Synaptotagmin.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300016

TMPYP4 exerted antitumor effects in human cervical cancer cells through activation of p38 mitogen-activated protein kinase
Provedor de dados: 48
Autores: Cheng,Ming&amp;#8209;Jun; Cao,Yun&amp;#8209;Gui.
Abstract  Background  The aim of the present study was to investigate the potential effects of the 5,10,15,20&amp;#8209;tetrakis (1&amp;#8209;meth&amp;#8209; ylpyridinium&amp;#8209;4&amp;#8209;yl) porphyrin (TMPyP4) on the proliferation and apoptosis of human cervical cancer cells and the underlying mechanisms by which TMPyP4 exerted its actions.  Results  After human cervical cancer cells were treated with different doses of TMPyP4, cell viability was determined by 3&amp;#8209;(4,5&amp;#8209;dimethyl&amp;#8209;2&amp;#8209;thiazolyl)&amp;#8209;2,5&amp;#8209;diphenyl&amp;#8209;2&amp;#8209;H&amp;#8209;tetrazolium bromide (MTT) method, the apoptosis was observed by flow cytometry (FCM), and the expression of p38 mitogen&amp;#8209;activated protein kinase...
Tipo: Journal article
Palavras-chave: TMPyP4; P38 MAPK; Human cervical cancer cells; Proliferation; Apoptosis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100217

Insulin is secreted upon glucose stimulation by both gastrointestinal enteroendocrine K-cells and L-cells engineered with the preproinsulin gene
Provedor de dados: 48
Autores: Encina,Gonzalo; Ezquer,Fernando; Conget,Paulette; Israel,Yedy.
Transgenic mice carrying the human insulin gene driven by the K-cell glucose-dependent insulinotropic peptide (GIP) promoter secrete insulin and display normal glucose tolerance tests after their pancreatic p-cells have been destroyed. Establishing the existence of other types of cells that can process and secrete transgenic insulin would help the development of new gene therapy strategies to treat patients with diabetes mellitus. It is noted that in addition to GIP secreting K-cells, the glucagon-like peptide 1 (GLP-1) generating L-cells share/ many similarities to pancreatic p-cells, including the peptidases required for proinsulin processing, hormone storage and a glucose-stimulated hormone secretion mechanism. In the present study, we demonstrate that...
Tipo: Journal article
Palavras-chave: Type 1 diabetes mellitus; Preproinsulin gene; Gastrointestinal enteroendocrine cells; K-cells; L-cells.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300012

Antioxidant, Antibacterial activity and Brine shrimp toxicity test of some Mountainous Lichens from Nepal
Provedor de dados: 48
Autores: Paudel,Babita; Datta Bhattarai,Hari; Prasad Pandey,Durga; Seoun Hur,Jae; Gyu Hong,Soon; Kim,Il-Chan; Han Yim,Joung.
A total of twenty four lichen species belonging to six families were collected from mountainous region of Nepal. The methanol extracts of each species were tested for antimicrobial and antioxidant activitiesin vitro. It was found that extracts of twenty one lichen species were active againstB. subtilis and seven species were active againstS. aureus. Similarly, in DPPH assay, three speciesPeltigera sp.,Cladonia sp., andCanoparmelia sp. showed comparable activity with commercial standard, BHA. In ABTS+ assay, extracts ofParmoterma sp.,Ramalina sp.,Peltigera sp. andCladonia sp. showed stronger activity than ascorbic acid. The observed data after comparison with previously published reports indicated that the high altitude lichens contain stronger antioxidant...
Tipo: Journal article
Palavras-chave: Antibacterial; Antioxidant; Nepal lichens; Free radicals.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400010

Is the Chilean Diet a Mediterranean-type Diet?
Provedor de dados: 48
Autores: ROZOWSKI,JAIME; CASTILLO,ÓSCAR.
Food intake in Chile has changed markedly in the last decades, showing an increase in fat consumption and presently a small fruit and vegetables intake. A parallel is made between the Chilean and Mediterranean diet (mainly the one from Spain, Italy, and Greece), both currently and from 50 years ago. The main differences and similarities are based on food availability. Although Chilean diet seems to be approaching the traditional Mediterranean diet of the 60's, there is concern about changes that are moving away from Chilean traditional diet and towards a western one. A new food pyramid for Chile is proposed based on the traditional Mediterranean-type diet
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200018

The foliar trichomes of Hypoestes aristata (Vahl) Sol. ex Roem. &amp;amp; Schult var aristata (Acanthaceae) a widespread medicinal plant species in tropical sub-Saharan Africa: with comments on its possible phylogenetic significance
Provedor de dados: 48
Autores: Bhatt,A; Naidoo,Y; Nicholas,A.
The micromorphology of foliar trichomes of Hypoestes aristata var. aristata was studied using stereo, light and scanning microscopy (SEM). This genus belongs to the advanced angiosperm family Acanthaceae, for which few micromorphological leaf studies exist. Results revealed both glandular and non-glandular trichomes, the latter being more abundant on leaf veins, particularly on the abaxial surface of very young leaves. With leaf maturity, the density of non-glandular trichomes decreased. Glandular trichomes were rare and of two types: long-stalked capitate and globose-like peltate trichomes. Capitate trichomes were observed only on the abaxial leaf surface, while peltate trichomes were distributed on both adaxial and abaxial leaf surfaces.
Tipo: Journal article
Palavras-chave: Acanthaceae; Glandular trichomes; Hypoestes aristata var. aristata; Medicinal plant; Scanning electron microscope.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400004

Effects of substrate water potential in root growth of Agave salmiana Otto ex Salm-Dyck seedlings
Provedor de dados: 48
Autores: PEÑA-VALDIVIA,CECILIA B; SÁNCHEZ-URDANETA,ADRIANA B.
The objective of this study was to test the hypothesis that root of maguey (Agave salmiana Otto ex Salm-Dyck) seedlings reacts during the first 24 h to low substrate water potential (Yw), by anatomical modifications. Three-4 cm root length seedlings were planted in vermiculite for 24 h at Yw between -0.03 and -2.35 MPa. Root dimensions, proline content and anatomy were evaluated. Substrate &amp;#968;w between -0.65 and -2.35 MPa did not significantly affect longitudinal root growth. However, proline content significantly increased from 1.6 to 2.1 emoles mg-1. Significant reductions of transverse root area (41 %), thickness of mucilage covering the epidermis (47 %), thickness of epidermis (between 15 and 46 %), area of the parenchyma (between 35 and 41 %)...
Tipo: Journal article
Palavras-chave: Maguey; Proline; Root anatomy; Root growth; Water deficit; Water stress.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200013

Measuring emotion in the voice during psychotherapy interventions: Apilot study
Provedor de dados: 48
Autores: MONETA,MARÍA E; PENNA,MARIO; LOYOLA,HUGO; BUCHHEIM,ANNA; KÁCHELE,HORST.
The voice as a representation of the psychic world of patients in psychotherapeutic interventions has not been studied thoroughly. To explore speech prosody in relation to the emotional content of words, voices recorded during a semi-structured interview were analyzed. The subjects had been classified according to their childhood emotional experiences with caregivers and their different attachment representations. In this pilot study, voice quality as spectral parameters extracted from vowels of the key word "mother" (Germán: "Mutter") were analyzed. The amplitude of the second harmonic was large relative to the amplitude of the third harmonic for the vowel "u" in the secure group as compared to the preoccupied group. Such differences might be related to...
Tipo: Journal article
Palavras-chave: Mother; Attachment; Vocal cues; Emotion.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400004

Effect of crocus sativus on gentamicin induced nephrotoxicity
Provedor de dados: 48
Autores: AJAMI,MARJAN; EGHTESADI,SHAHRIAR; PAZOKI-TOROUDI,HAMIDREZA; HABIBEY,ROUHOLLAH; EBRAHIMI,SOLTAN AHMED.
Crocus sativus, known as saffron, is used in folk medicine for treatment of different types of diseases, and its anti-inflammatory and free radical scavenging activities have been demonstrated. The present study evaluated gentamicin nephrotoxicity in saffron treated rats. Male Wistar rats (200-250g) were treated with saffron (40 or 80 mg/k/d) for 10 days, or saffron (40 or 80 mg/ kg/d) for 10 days and gentamicin 80 mg/kg/d for five days, starting from day 6. At the end of treatment, blood samples were taken for measurement of serum creatinine (SCr) and BUN. The left kidney was prepared for histological evaluation and the right kidney for Malondialdehyde (MDA) measurement. Gentamicin 80 (mg/k/d) increased SCr, BUN and renal tissue levels of MDA and induced...
Tipo: Journal article
Palavras-chave: Saffron; Malondialdehyde (MDA); Aminoglycoside; Renal failure.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100010

Quantitative genetic variation of metabolism in the nymphs of the sand cricket, Gryllus firmus, inferred from an analysis of inbred-lines
Provedor de dados: 48
Autores: NESPOLO,ROBERTO F; CASTAÑEDA,LUIS E; ROFF,DEREK A.
Compared with morphological and life history traits, quantitative genetic variation of metabolic and related traits in animals has been poorly studied. We used flow-through VC0(2) respirometry and simultaneous activity measurement on nymphs of the sand cricket {Gryllus firmus) from inbred lines to estimate broad-sense heritability of four metabolic variables. In addition, we measured a number of linear dimensions in the adults from the same inbred lines. There were significant multivariate effects of inbred lines for all traits and broad-sense heritability for physiological traits was 4.5%, 5.2%, 10.3% and 8.5% for average, resting, minimum and maximum C0(2) production in nymphs, respectively. Though the MANOVA indicated significant genetic variation among...
Tipo: Journal article
Palavras-chave: Broad-sense heritability; Metabolic rate; Insects; Inbred lines; Through-flow C0(2) respirometry.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100001

Low concentrations of doxycycline attenuates FasL-induced apoptosis in HeLa cells
Provedor de dados: 48
Autores: Yoon,Jung Mi; Koppula,Sushruta; Huh,Se Jong; Hur,Sun Jin; Kim,Chan Gil.
BACKGROUND: Doxycycline (DC) has been shown to possess non-antibiotic properties including Fas/Fas Ligand (FasL)-mediated apoptosis against several tumor types in the concentration range of 10-40 &amp;#956;g/mL. However, the effect of DC in apoptotic signaling at much low concentrations was not studied. METHODS: The present study investigated the attenuation effect of low dose of DC on FasL-induced apoptosis in HeLa cell by the methods of MTT assay, fluorescence microscopy, DNA fragmentation, flow cytometry analysis, and western blotting. RESULTS AND CONCLUSION: In the present findings we showed that low concentration of DC (&lt;2.0 &amp;#956;g/mL) exhibited protective effects against FasL-induced apoptosis in HeLa cells. FasL treatment to HeLa cells...
Tipo: Journal article
Palavras-chave: Apoptosis; Caspase; Cisplatin; FasL; Hydrogen peroxide; Tetracycline.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100038

Structural-functional analysis of the oligomeric protein R-phycoerythrin
Provedor de dados: 48
Autores: MARTÍNEZ-OYANEDEL,JOSÉ; CONTRERAS-MARTEL,CARLOS; BRUNA,CAROLA; BUNSTER,MARTA.
The structure of phycobiliproteins and their spatial organization in the phycobilisome provide the environment for high efficiency in light harvesting and conduction towards photosystem II. This article focuses on the analysis of R-phycoerythrin, a light harvesting hexameric phycobiliprotein that is part of the phycobilisomes. The interaction surfaces and the environment of the chromophores of R-phycoerythrin were studied in order to explain its structural stability and spectroscopic sensitivity, properties revealed by perturbation experiments. Three interaction surfaces are described (ab), (ab)3 and (ab)6. The analysis shows the importance of a subunits in the interaction between trimers, the homodimeric nature of the monomer (ab) and also the presence of...
Tipo: Journal article
Palavras-chave: 1eyx; Anchor points; Interaction surfaces; Stability; Spectroscopic sensitivity.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500003

Summary of the Third International Workshop on Iron and Copper Homeostasis
Provedor de dados: 48
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100001

The p. N103K mutation of leptin (LEP) gene and severe early onset obesity in Pakistan
Provedor de dados: 48
Autores: Shabana,; Hasnain,Shahida.
BACKGROUND: Obesity is a complex disorder and has been increasing globally at alarming rates including Pakistan. However, there is scarce research on understanding obesity genetics in Pakistan. Leptin is a hormone secreted by adipocytes in response to satiety and correlates with body weight. Any mutations in the LEP gene have an adverse effect on energy regulation pathway and lead to severe, early onset obesity. To date, only eight mutations have been described in the LEP gene of which p. N103K is one. METHODS: We aimed to analyze the prevalence of this mutation in Pakistani subjects. A total of 475 subjects were genotyped by PCR-RFLP analysis and their serum profiling was done. RESULTS: Results showed that this mutation was present only in one male child...
Tipo: Journal article
Palavras-chave: Obesity; Leptin; P. N103K; Pakistan.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100023

Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway
Provedor de dados: 48
Autores: Jang,Yongwoo; Kim,Eun-Kyung; Shim,Won-Sik; Song,Ki-Min; Kim,Sung Min.
BACKGROUND: The fetus is surrounded by the amniotic fluid (AF) contained by the amniotic sac of the pregnant female. The AF is directly conveyed to the fetus during pregnancy. Although AF has recently been reported as an untapped resource containing various substances, it remains unclear whether the AF could influence fetal neurodevelopment. RESULTS: We used AF that was extracted from embryos at 16 days in pregnant SD rat and exposed the AF to the neural cells derived from the embryos of same rat. We found that the treatment of AF to cortical neurons increased the phosphorylation in ERK1/2 that is necessary for fetal neurodevelopment, which was inhibited by the treatment of MEK inhibitors. Moreover, we found the subsequent inhibition of glycogen synthase...
Tipo: Journal article
Palavras-chave: Amniotic fluid; Extracellular signal-regulated kinase (ERK); Glycogen synthase kinase-3 (GSK-3); Neural clustering; Neurosphere; Fetal development.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100044

Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation
Provedor de dados: 48
Autores: FERNÁNDEZ,VIRGINIA; MASSA,LAURA; QUIÑONES,LUIS; SIMON-GIAVAROTTI,KARIN A; GIAVAROTTI,LEANDRO; D'ALMEIDA,VÂNIA; AZZALIS,LIGIA A; JUNQUEIRA,VIRGINIA BC; VIDELA,LUIS A.
Liver microsomal cytochrome P4502E1-dependent p-nitrophenol (PNP) hydroxylation and expression of cytochrome P4502E1 were studied in rats subjected to g-hexachlorocyclohexane (HCCH) or L-3,3,,5-triiodothyronine (T3) administration as a possible mechanism contributing to superoxide radical (O2.-) generation. HCCH treatment (a single dose of 40 mg/kg body wt) produced a 43% increase in the content of total cytochrome P450, whereas T3 (daily doses of 0.1 mg/kg body wt for two consecutive days) led to a 37% decrease. NADPH-dependent O2.- generation was elevated by HCCH and T3, expressed as either per mg of protein or per nmol of cytochrome P450, with a 135% enhancement in the O2.- production/superoxide dismutase (SOD) activity ratios being observed in both...
Tipo: Journal article
Palavras-chave: G-Hexachlorocyclohexane L-3; 3'; 5-Triiodothyronine Superoxide radical Cytochrome P4502E1 Rat liver.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300007

PANELES 1
Provedor de dados: 48
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000300006

Coexistence of cytotypes and chromosomal mosaicism in Hoplias malabaricus (Characiformes, Erythrinidae)
Provedor de dados: 48
Autores: DA ROSA,RENATA; RUBERT,MARCELÉIA; BERTOLLO,LUIZ A C; MARTINS-SANTOS,ISABEL C; GIULIANO-CAETANO,LUCIA.
Karyotypes of seventeen Hoplias malabaricus specimens, collected in the fish culture station of UNOPAR (University of Northern Paraná), were analyzed. The station is in the Claro River system in the Tibagi River basin. Two distinct and coexistent karyotype forms (cytotypes) were identified, comprising either 42 chromosomes (cytotype A) or 40 chromosomes (cytotype C), both presenting metacentric and submetacentric chromosomes. In two specimens, one male and one female, it was not possible to characterize a modal diploid number because different cell lines were observed, with a predominance of 2n=41 and 2n=42 chromosomes at a frequency of 38.24% and 41.12%, respectively. The karyotype with 2n=41 showed some putative monosomic and trisomic chromosomes, while...
Tipo: Journal article
Palavras-chave: Hoplias malabaricus; Chromosomal mosaicism; Coexistence of cytotypes; RAPD; Reproductive isolation.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300003

Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells
Provedor de dados: 48
Autores: MENDIBURO,MARÍA JOSE; FLORES,SEBASTIÁN; PIZARRO,FERNANDO; ARREDONDO,MIGUEL.
Heme oxygenase-1 is a microsomal enzyme that, when induced by stress, protects the cells from oxidative injury. Heme oxygenase-1 participates in the cleavage of the heme ring producing biliverdin, CO and ferrous Fe. The released Fe becomes part of intracellular Fe pool and can be stored in ferritin or released by an iron exporter. The mechanism by which heme enters cells is not completely understood, although it had been suggested that it might be internalized by an endocytosis process. In this study, we expressed a full-length Heme oxygenase-1 cDNA in Caco-2 cells and measured intracellular iron content, heme-iron uptake and transport and immunolocalization of heme oxygenase-1 in these cells. We found that heme oxygenase-1 expressing cells showed...
Tipo: Journal article
Palavras-chave: Iron; Heme; Heme oxygenase; Caco-2 cells.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100023

The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3
Provedor de dados: 48
Autores: Li,JinLong; Li,Hui; Zhang,ZhaoLi; Wang,NianYue; Zhang,Yong Chen.
CONTEXT: Trichosanthin produced in the root tube of Trichosanthes kirilowii shows anti-tumor activity on a series of cancer cells including Hela, MCF-7, HL-60. But there is little information about its effect on the carcinogenesis of prostate cancer. OBJECTIVE: This work was designed to study the role of trichosanthin on prostate cancer cells PC3. MATERIALS AND METHODS: Trichosanthin was expressed in BL21 strain and purified by affinity chromatography. MTT assay was designed to determine the effect of trichosanthin on growth of PC3 cells at doses of 10, 20, 40, 60, 80, and 120 &amp;#956;g/ml.Then the effect of 50 &amp;#956;g/ml rTCS alone or combined with 2 &amp;#956;&amp;#924; IL-2 on PC3 cell proliferation was analyzed. And the mechanism of rTCS was...
Tipo: Journal article
Palavras-chave: Trichosanthin; Interleukin IL-2; Prostate cancer cells PC3; Tumor.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100021

Evaluation of foliar fungal endophyte diversity and colonization of medicinal plant Luehea divaricata (Martius et Zuccarini)
Provedor de dados: 48
Autores: Bernardi-Wenzel,Juliana; García,Adriana; Filho,Celso J R; Prioli,Alberto J; Pamphile,João A.
Endophyte microorganisms are organisms that live inside plants without causing any apparent damage to their hosts. Since all plants exhibit endophyte microorganisms, it is believed that mutual association is of great importance in nature. Luehea divaricata (Martius &amp;amp; Zuccarini), known popularly in Brazil as agoita-cavalo, is a big-sized tree with a wide distribution in the country that possesses medicinal qualities for: dysentery, leucorrhea, rheumatism, blennorrhoea, tumors, bronchitis, and depuration. This research aims at isolating and molecularly characterizing fungi isolates from L. divaricata by sequence analysis of ITS1-5.8S-ITS2 rDNA. Further, the colonization of endophyte in the host plant by Light and Scanning Electron Microscopy will...
Tipo: Journal article
Palavras-chave: Endophyte fungi; Luehea divaricata; Diversity; RDNA sequencing; Scanning Electron Microscopy.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400001

Neuroethics: the pursuit of transforming medical ethics in scientific ethics
Provedor de dados: 48
Autores: Figueroa,Gustavo.
Ethical problems resulting from brain research have given rise to a new discipline termed neuroethics, representing a new kind of knowledge capable of discovering the neural basis for universal ethics. The article (1) tries to evaluate the contributions of neuroethics to medical ethics and its suitability to outline the foundations of universal ethics, (2) critically analyses the process of founding this universal ethic. The potential benefits of applying neuroimaging, psy-chopharmacology and neurotechnology have to be carefully weighed against their potential harm. In view of these questions, an intensive dialogue between neuroscience and the humanities is more necessary than ever.
Tipo: Journal article
Palavras-chave: Neuroethics; Free will; Mind-body problem; Neuroscience.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100011

Erratum to: Superoxide dismutase and catalase: tissue activities and relation with age in the long-lived species Margaritifera margaritifera
Provedor de dados: 48
Autores: Fernández,Carlos; Ferreira,Eloísa; San Miguel,Eduardo; Fernández-Briera,Almudena.
Tipo: Journal article
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100035

Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells
Provedor de dados: 48
Autores: Du,Maotao; Zhang,Zhong; Gao,Tao.
Abstract  Background Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells.  Methods Melanoma cell lines WM266-4 and A2058 were treated either with or without piceatannol. Cell viability and cell apoptosis were assessed by using MTT and Annexin V/PI assay, respectively. Cells were transfected with specific...
Tipo: Journal article
Palavras-chave: Piceatannol; Apoptosis; MicroRNA-181-a; Melanoma cells.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100226

A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
Provedor de dados: 48
Autores: MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN.
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the...
Tipo: Journal article
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010

Influence of rootstocks on growth, yield, fruit quality and leaf mineral element contents of pear cv. 'Santa Maria' in semi-arid conditions
Provedor de dados: 48
Autores: Ikinci,Ali; Bolat,Ibrahim; Ercisli,Sezai; Kodad,Ossama.
BACKGROUND: Rootstocks play an essential role to determining orchard performance of fruit trees. Pyrus communisand Cydonia oblonga are widely used rootstocks for European pear cultivars. The lack of rootstocks adapted to different soil conditions and different grafted cultivars is widely acknowledged in pear culture. Cydonia rootstocks (clonal) and Pyrus rootstocks (seedling or clonal) have their advantages and disadvantages. In each case, site-specific environmental characteristics, specific cultivar response and production objectives must be considered before choosing the best rootstock. In this study, the influence of three Quince (BA 29, Quince A = MA, Quince C = MC) and a local European pear seedling rootstocks on the scion yield, some fruit quality...
Tipo: Journal article
Palavras-chave: TCSA; BA-29; MA; MC; Pear seedlings; Pyrus communis L; Cydonia oblonga L.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100065

Diet and Endothelial Function
Provedor de dados: 48
Autores: CUEVAS,ADA M; GERMAIN,ALFREDO M.
Endothelial dysfunction is one of the earliest events in atherogenesis. A consequence of endothelial damage is a lower availability of nitric oxide (NO), the most potent endogenous vasodilator. NO inhibits platelet aggregation, smooth muscle cell proliferation and adhesion of monocytes to endothelial cells. Endothelial dysfunction is present in patients with cardiovascular disease and/or coronary risk factors, such as hypertension, dyslipidemia, diabetes, smoking or hyperhomocysteinemia. At present, soluble markers and high resolution ultrasound of the brachial artery, have provided simple tools for the study of endothelial function and the effects of several interventions. It has been demonstrated that dietary factors may induce significant changes on...
Tipo: Journal article
Palavras-chave: Diet; Endothelial function; Nitric oxide; Vascular reactivity.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200008

Molecular signature of cancer stem cells isolated from prostate carcinoma and expression of stem markers in different Gleason grades and metastasis
Provedor de dados: 48
Autores: Castellón,Enrique A; Valenzuela,Rodrigo; Lillo,Jorge; Castillo,Viviana; Contreras,Héctor R; Gallegos,Iván; Mercado,Alejandro; Huidobro,Christian.
Prostate cancer (PCa) is the most frequently diagnosed malignancy in men worldwide. Chemotherapy response is very poor and resistance to hormone-based treatments is frequent in advances stages. Recently, tumor-initiating cells or cancer stem cells (CSCs) have been identified in several cancers, including PCa. These cells are thought to be responsible for therapy resistance, relapse and metastasis. In the present work, enriched populations of CSCs were obtained using a mixed procedure that included differential clone-forming ability, sphere growing induction (prostatospheres) and magnetic-associated cell sorting (MACS). Also, stem marker expression was determined in PCa biopsies of different histological grades and metastasis samples. The signature for stem...
Tipo: Journal article
Palavras-chave: Prostate cancer; Cancer stem cell; Spheres cultures.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300011

Effects of plant growth promoting rhizobacteria (PGPR) on rooting and root growth of kiwifruit (Actinidia deliciosa) stem cuttings
Provedor de dados: 48
Autores: ERTURK,YASAR; ERCISLI,SEZAI; HAZNEDAR,AYHAN; CAKMAKCI,RAMAZAN.
The effects of plant growth promoting rhizobacteria (PGPR) on the rooting and root growth of semi-hardwood and hardwood kiwifruit stem cuttings were investigated. The PGPR used were Bacillus RC23, Paenibacillus polymyxa RC05, Bacillus subtilis OSU142, Bacillus RC03, Comamonas acidovorans RC41, Bacillus megaterium RC01 and Bacillus simplex RC19. All the bacteria showed indole-3-acetic acid (IAA) producing capacity. Among the PGPR used, the highest rooting ratios were obtained at 47.50% for semi-hardwood stem cuttings from Bacillus RC03 and Bacillus simplex RC19 treatments and 42.50% for hardwood stem cuttings from Bacillus RC03. As well, Comamonas acidovorans RC41 inoculations indicated higher value than control treatments. The results suggest that these...
Tipo: Journal article
Palavras-chave: PGPR; Stem cuttings; Kiwifruit; Rooting; Auxin.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100011

Isolation, sequencing and phylogenetic analysis of the hemagglutinin, neuraminidase and nucleoprotein genes of the Chilean equine influenza virus subtypes H7N7 and H3N8
Provedor de dados: 48
Autores: Müller,Ilse; Jaureguiberry,Beltrán; Valenzuela,Pablo D.T.
We report here on the isolation and sequencing of the hemagglutinin, neuraminidase and nucleoprotein genes of the Chilean equine influenza virus subtypes H7N7 (A&amp;#8260;equi-1&amp;#8260;Santiago&amp;#8260;77, Sa77) and H3N8 (A&amp;#8260;equi-2&amp;#8260;Santiago&amp;#8260;85, Sa85) . The sequences obtained allowed a variability analysis, which indicated significant differences when compared with other isolates. We found that Chilean isolates are more similar to the North American variety than to European isolates. Isolate Sa77 is a good candidate for inclusion in a vaccine as it is the latest isolate of the subtype H7N7 and is probably better-adapted to the equine host. Isolate Sa85, of subtype H3N8, also appears to be a good candidate since it has no...
Tipo: Journal article
Palavras-chave: Equine influenza; Hemagglutinin; Neuraminidase; Nucleoprotein genes.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100008

Heavy Metal Resistance Strategies of Acidophilic Bacteria and Their Acquisition: Importance for Biomining and Bioremediation
Provedor de dados: 48
Autores: Navarro,Claudio A; von Bernath,Diego; Jerez,Carlos A.
Microbial solubilizing of metals in acid environments is successfully used in industrial bioleaching of ores or biomining to extract metals such as copper, gold, uranium and others. This is done mainly by acidophilic and other microorganisms that mobilize metals and generate acid mine drainage or AMD, causing serious environmental problems. However, bioremediation or removal of the toxic metals from contaminated soils can be achieved by using the specific properties of the acidophilic microorganisms interacting with these elements. These bacteria resist high levels of metals by using a few "canonical" systems such as active efflux or trapping of the metal ions by metal chaperones. Nonetheless, gene duplications, the presence of genomic islands, the...
Tipo: Journal article
Palavras-chave: Heavy metal resistance; Genomic islands; Environmental bacteria; Biomining; Metal bioremediation; Mobile genetic elements.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400008

Fine structure of the eggs of blowflies Aldrichina grahami and Chrysomya pacifica (Diptera: Calliphoridae)
Provedor de dados: 48
Autores: SUKONTASON,KOM; SUKONTASON,KABKAEW L.; PIANGJAI,SOMSAK; CHOOCHOTE,WEJ; BOONCHU,NOPPAWAN; CHAIWONG,TARINEE; KURAHASHI,HIROMU.
We report here the fine structure of the eggs of blowflies Aldrichina grahami (Aldrich) and Chrysomya pacifica Kurahashi. For A. grahami, the plastron is wide and extends to almost the entire length of the eggs. The plastron near the micropyle is truncated. The polygonal patterns of chorionic sculpture bear a distinct swollen boundary. Regarding C. pacifica, the plastron is narrow and extends to almost the entire length of the eggs. The plastron near the micropyle bifurcates to a Y-shape, but the arms of the 'Y' are short. Information presented herein allows some distinctive features to differentiate among eggs of blowfly species.
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300012

Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
Provedor de dados: 48
Autores: POLANCO,RUBÉN; CANESSA,PAULO; RIVAS,ALEXIS; LARRONDO,LUIS F; LOBOS,SERGIO; VICUÑA,RAFAEL.
In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1...
Tipo: Journal article
Palavras-chave: Acel; Basidiomycete; Copper; Phanerochaete chrysosporium.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007

Genomic organization of nucleolin gene in carp fish: Evidence for several genes
Provedor de dados: 48
Autores: QUEZADA,CLAUDIA; NAVARRO,CRISTINA; SAN MARTÍN,RODY; ÁLVAREZ,MARCO; MOLINA,ALFREDO; VERA,M. INÉS.
The protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. Here we report that at least three different genes codify for this protein in carp fish (Cyprinus carpio). This is the first description of the genomic organization of nucleolin in a teleost. The carp nucleolin gene includes 8.8 kb and contains 16 exons. Promoter cis regulatory elements are similar to constitutive genes, i.e., a putative TATA box, three G/C boxes, and three pyrimidine-rich boxes. As in other species, carp nucleolin gene introns host three snoRNA codifying sequences: U23 from the H/ACA family and two C/D box snoRNAs, U20 and U82. Both U20 and U82 span a complementary sequence with carp 18S rRNA. Additionally,...
Tipo: Journal article
Palavras-chave: Nucleolin; Nucleolus; U snoRNAs; RRNA 18S; Carp fish.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017

Prevalence of the 35delG mutation in the GJB2 gene in two samples of non-syndromic deaf subjects from Chile
Provedor de dados: 48
Autores: Cifuentes,Lucía; Arancibia,Margarita; Torrente,Mariela; Acuña,Mónica; Farfán,Corina; Ríos,Carolina.
Hearing loss is the most common inherited sensorial deficiency in humans; about 1 in 1000 children suffer from severe or profound hearing loss at birth. Mutations in the GJB2 gene are the most common cause of prelingual, non-syndromic autosomal recessive deafness in many populations; the c.35delG mutation is the most common in Caucasian populations. The frequency of the c.35delG mutation was estimated in two samples of deaf patients from Santiago, Chile. Unrelated non-syndromic sensorioneural deaf patients were examined: Group 1 consisted of 47 unrelated individuals with neurosensory deafness referred to the Chilean Cochlear Implant Program; Group 2 included 66 school children with prelingual deafness attending special education institutions for deaf...
Tipo: Journal article
Palavras-chave: Genetic deafness; Hereditary hearing loss; 35delG mutation frequency; GJB2 mutations; Chilean population.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300003

New Insights into the Roles of Megalin/LRP2 and the Regulation of its Functional Expression
Provedor de dados: 48
Autores: Marzolo,María-Paz; Farfán,Pamela.
Since the discovery of the low-density lipoprotein receptor (LDLR) and its association with familial hypercholesterolemia in the early 1980s, a family of structurally related proteins has been discovered that has apolipoprotein E as a common ligand, and the broad functions of its members have been described. LRP2, or megalin, is a member of the LDLR family and was initially called gp330. Megalin is an endocytic receptor expressed on the apical surface of several epithelial cells that internalizes a variety of ligands including nutrients, hormones and their carrier proteins, signaling molecules, morphogens, and extracellular matrix proteins. Once internalized, these ligands are directed to the lysosomal degradation pathway or transported by transcytosis...
Tipo: Journal article
Palavras-chave: Alzheimer's Disease; Cubilin; Diabetic nephropathy; FXR; Gallstone disease; GSK3; Lowe syndrome; Megalin; PPAR; RAP; Regeneration.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100012

Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis
Provedor de dados: 48
Autores: Wang,Hui; Liu,Zheng; Zhang,Wenxiu; Yuan,Ziao; Yuan,Hongyi; Liu,Xueting; Yang,Chunwen; Guan,Weijun.
BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 &amp;#956;&amp;#924;) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It...
Tipo: Journal article
Palavras-chave: Siberian tiger; Cadmium; Apoptosis; Fibroblast; Calcium.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100042

Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells
Provedor de dados: 48
Autores: TORO,ANDRÉS; ARREDONDO,CRISTIAN; CORDOVA,GONZALO; ARAYA,CLAUDIA; PALACIOS,JOSÉ L; VENEGAS,ALEJANDRO; MORITA,MASASHI; IMANAKA,TSUNEO; SANTOS,MANUEL J.
Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-Golgi transit in peroxisome biogenesis in mammalian cells, we evaluated the biogenesis of three peroxisomal membrane proteins (PMPs): ALDRP (adrenoleukodystrophy related protein), PMP70 and Pex3p in CHO cells. We constructed chimeric genes encoding these PMPs and green fluorescent protein (GFP), and transiently transfected them to wild type and mutant CHO cells, in which normal peroxisomes were replaced by peroxisomal membrane ghosts. The...
Tipo: Journal article
Palavras-chave: Adrenoleukodystrophy; ALDRP; Endoplasmic reticulum; Peroxisome biogenesis; Pex3p and PMP70.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200014

Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae
Provedor de dados: 48
Autores: GÖTTE,MARTIN; STADTBÄUMER,ANDREA.
The molecular mechanisms of vesicular protein transport in eukaryotic cells are highly conserved. Members of the syntaxin family play a pivotal role in the membrane fusion process. We have expressed rat syntaxin 6 and its cytoplasmic domain in wild-type and pep12 mutant strains of Saccharomyces cerevisiae to elucidate the role of the syntaxin 6-dependent vesicular trafficking step in yeast. Immunofluorescence microscopy revealed a punctate, Golgi-like staining pattern for syntaxin 6, which only partially overlapped with Pep12p in wild-type yeast cells. In contrast to Pep12p, syntaxin 6 was not mislocalized to the vacuole upon expression from 2 micron vectors, which might be attributed to conserved sorting and retention signals. Syntaxin 6 was not capable...
Tipo: Journal article
Palavras-chave: Vesicular transport; SNARE; Syntaxin; Endosomal trafficking; Vacuole protein sorting; PEP12.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300009

Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
Provedor de dados: 48
Autores: MANCUSO,MONIQUE; AVENDAÑO-HERRERA,RUBÉN; ZACCONE,R; TORANZO,ALICIA E; MAGARIÑOS,BEATRIZ.
This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover,...
Tipo: Journal article
Palavras-chave: Photobacterium damselae ssp; Piscicida; RAPD; ERIC-PCR; REP-PCR.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100009

Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers
Provedor de dados: 48
Autores: LINDER,MARIA C; MORIYA,MIZUE; WHON,ANNE; KASSA,AZEB; GILLEY,CYNTHIA.
Two aspects of the mechanisms by which iron is absorbed by the intestine were studied in the Caco2 cell model, using 59Fe(II)-ascorbate. Data showing the importance of vesicular processes and cycling of apotransferrin (apoTf) to uptake and overall transport of Caco2 cell monolayers (or basolateral 59Fe release) were obtained by comparing effects of: a) adding apoTf to the basal chamber; b) adding vesicular transport inhibitors; or c) cooling to 4ºC. These showed that apoTf may be involved in as much as half of Fe transfer across the basolateral membrane, and that vesicular processes may also play a role in non-apoTf-dependent Fe transport. Studies were initiated to examine potential interactions of other metal ions with Fe(II) via DMT1. Kinetic data showed...
Tipo: Journal article
Palavras-chave: Intestinal iron absorption; Caco2 cells; Apotransferrin; Vesicular transport; Copper; Manganese.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100016

Vitamin C and oxidative stress in the seminiferous epithelium
Provedor de dados: 48
Autores: Angulo,Constanza; Maldonado,Rodrigo; Pulgar,Eduardo; Mancilla,Héctor; Córdova,Alex; Villarroel,Franz; Castro,Maite A; Concha,Ilona I.
In this article, we focus on the fundamental role of vitamin C transporters for the normal delivery of vitamin C to germ cells in the adluminal compartment of seminiferous tubules. We argue that the redox status within spermatozoa or in semen is partly responsible for the etiology of infertility. In this context, antioxidant defence plays a critical role in male fertility. Vitamin C, a micronutrient required for a wide variety of metabolic functions, has long been associated with male reproduction. Two systems for vitamin C transport have been described in mammals. Facilitative hexose transporters (GLUTs), with 14 known isoforms to date, GLUT1-GLUT14, transport the oxidized form of vitamin C (dehydroascorbic acid) into the cells. Sodium ascorbic acid...
Tipo: Journal article
Palavras-chave: Ascorbic acid; Oxidative stress; GLUT; Sertoli cells; SVCT; Vitamin C transporters.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200009

Hepato-protective effect of rutin via IL-6/STAT3 pathway in CCl4-induced hepatotoxicity in rats
Provedor de dados: 48
Autores: Hafez,Mohamed M; Al-Harbi,Naif O; Al-Hoshani,Ali Rashed; Al-hosaini,Khaled A; Al Shrari,Shakir D; Al Rejaie,Salim S; Sayed-Ahmed,Mohamed M; Al-Shabanah,Othman A.
BACKGROUND: Carbon tetrachloride (CCl4) induces hepatotoxicity in animal models, including the increased blood flow and cytokine accumulation that are characteristic of tissue inflammation. The present study investigates the hepato-protective effect of rutin on CCl4-induced hepatotoxicity in rats. RESULTS: Forty male Wistar rats were divided into four groups. Group I (control group) received 1 mL/kg of dimethyl sulfoxide intragastrically and 3 mL/kg olive oil intraperitoneally twice a week for 4 weeks. Group II received 70 mg/ kg rutin intragastrically. Groups III and IV received CCl4 (3 mL/kg, 30 % in olive oil) intraperitoneally twice a week for 4 weeks. Group IV received 70 mg/kg rutin intragastrically after 48 h of CCl4 treatment. Liver enzyme levels...
Tipo: Journal article
Palavras-chave: Hepatotoxicity; Rutin; Inflammatory cytokine; MEK5; FADD; STAT3; JAK genes expression.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100030

Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee
Provedor de dados: 48
Autores: González-Alvarez,Rafael; Garza-Rodriguez,María de Lourdes; Delgado-Enciso,Iván; Treviño-Alvarado,Victor Manuel; Canales-Del-Castillo,Ricardo; Martínez-De-Villarreal,Laura Elia; Lugo-Trampe,Ángel; Tejero,María Elizabeth; Schlabritz-Loutsevitch,Natalia E.; Rocha-Pizaña,María Del Refugio; Cole,Shelley A.; Reséndez-Pérez,Diana; Moises-Alvarez,Mario; Comuzzie,Anthony G.; Barrera-Saldaña,Hugo Alberto; Garza-Guajardo,Raquel; Barboza-Quintana,Oralia; Rodríguez-Sánchez,Irám Pablo.
BACKGROUND: Chemerin, encoded by the retinoic acid receptor responder 2 (RARRES2) gene is an adipocytesecreted protein with autocrine/paracrine functions in adipose tissue, metabolism and inflammation with a recently described function in vascular tone regulation, liver, steatosis, etc. This molecule is believed to represent a critical endocrine signal linking obesity to diabetes. There are no data available regarding evolution of RARRES2 in non-human primates and great apes. Expression profile and orthology in RARRES2 genes are unknown aspects in the biology of this multigene family in primates. Thus; we attempt to describe expression profile and phylogenetic relationship as complementary knowledge in the function of this gene in primates. To do that, we...
Tipo: Journal article
Palavras-chave: Retinoic acid receptor; Responder protein; Primate; Chemerin; TIG2; Tazarotene-induced gene 2 protein; Gene expression.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100031

From autopoiesis to neurophenomenology: Francisco Varela's exploration of the biophysics of being
Provedor de dados: 48
Autores: RUDRAUF,DAVID; LUTZ,ANTOINE; COSMELLI,DIEGO; LACHAUX,JEAN-PHILIPPE; LE VAN QUYEN,MICHEL.
This paper reviews in detail Francisco Varela's work on subjectivity and consciousness in the biological sciences. His original approach to this "hard problem" presents a subjectivity that is radically intertwined with its biological and physical roots. It must be understood within the framework of his theory of a concrete, embodied dynamics, grounded in his general theory of autonomous systems. Through concepts and paradigms such as biological autonomy, embodiment and neurophenomenology, the article explores the multiple levels of circular causality assumed by Varela to play a fundamental role in the emergence of human experience. The concept of biological autonomy provides the necessary and sufficient conditions for characterizing biological life and...
Tipo: Journal article
Palavras-chave: Autonomous systems; Brain dynamics; Consciousness; Embodiment; Francisco Varela; Neurophenomenology.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100005

Dr. Rafael Vicuña: Miembro de Número de la Pontificia Academia de Ciencias del Vaticano
Provedor de dados: 48
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300003

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae
Provedor de dados: 48
Autores: Hernandez,Pedro P; Undurraga,Cristian; Gallardo,Viviana E; Mackenzie,Natalia; Allende,Miguel L; Reyes,Ariel E.
Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae...
Tipo: Journal article
Palavras-chave: Acute exposure; Apoptosis; Copper; Early life stage; Heat shock; Protein-70; Zebrafish.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100002

Influence of time at which oxytocin is administered during labor on uterine activity and perinatal death in pigs
Provedor de dados: 48
Autores: MOTA-ROJAS,DANIEL; VILLANUEVA-GARCÍA,DINA; VELAZQUEZ-ARMENTA,ELVIA Y; NAVA-OCAMPO,ALEJANDRO A; RAMÍREZ-NECOECHEA,RAMIRO; ALONSO-SPILSBURY,MARÍA; TRUJILLO,MARÍA E.
Oxytocin is extensively used to induce or augment uterine contractions, especially to facilitate the third stage of labor in humans. Administration of oxytocin to parturient sows reduces duration of labor whereas mortality of the offspring may remain unchanged. This study aimed to evaluate whether time of administration of oxytocin during parturition may alter the uterine response and fetal outcomes. Two hundred parturient sows were randomly assigned to intramuscularly receive either saline solution (control group) or oxytocin 0.083 IU/kg immediately after the delivery of the 1st, 4th or 8th piglet (groups O-1, 0-4 and 0-8, respectively). Uterine effects and fetal outcomes were registered in all groups. The duration of labor was 20-40 min shorter (P &lt;...
Tipo: Journal article
Palavras-chave: Fetal distress; Fetal monitoring; Obstetric labor; Oxytocin; Uterine contractions.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100006

Wavelet and Fourier analysis of ventricular and main arteries pulsations in anesthetized dogs
Provedor de dados: 48
Autores: JIMÉNEZ,RAÚL F; TORRES,PATRICIO; GÜNTHER,BRUNO; MORGADO,ENRIQUE; JIMÉNEZ,CLAUDIA A.
The purpose of this study was to characterize time-frequency behavior using the Continuous Wavelet Transform (CWT) and Fast Fourier Transform (FFT) to analyze ventricular and arterial pressure signals from anesthetized mongrel dogs. Both ventricular and arterial pressure pulsations were recorded using catheter-tip manometers and the CWT was applied to these signals to obtain module coefficients, associated contours, and the 3-D representation of these modules. FFT was applied to obtain the Fourier spectrum. The mathematical analysis of the cardiovascular pressure pulsations permitted the identification of the evolution of the frequency components for the aortic and pulmonary valve functions as well as the intra-ventricular and respiratory influences on the...
Tipo: Journal article
Palavras-chave: Wavelet transform; Fourier transform; Time-frequency-amplitude; Ventricular and arterial pressures.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300008

Iron homeostasis in the lung
Provedor de dados: 48
Autores: GHIO,ANDREW J; TURI,JENNIFER L; YANG,FUNMEI; GARRICK,LAURA M; GARRICK,MICHAEL D.
Iron is essential for many aspects of cellular function. However, it also can generate oxygen-based free radicals that result in injury to biological molecules. For this reason, iron acquisition and distribution are tightly regulated. Constant exposure to the atmosphere results in significant exposure of the lungs to catalytically active iron. The lungs have a mechanism for detoxification to prevent associated generation of oxidative stress. Those same proteins that participate in iron uptake in the gut are also employed in the lung to transport iron intracellularly and sequester it in an inactive form within ferritin. The release of metal is expedited (as transferrin and ferritin) from lung tissue to the respiratory lining fluid for clearance by the...
Tipo: Journal article
Palavras-chave: Lung diseases; Oxidative stress; SLC11A2 protein; SLC40A1 protein.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100008

Diabetic retinopathy: could the alpha-1 antitrypsin be a therapeutic option?
Provedor de dados: 48
Autores: Ortiz,Gustavo; Salica,Juan P; Chuluyan,Eduardo H; Gallo,Juan E.
Diabetic retinopathy is one of the most important causes of blindness. The underlying mechanisms of this disease include inflammatory changes and remodeling processes of the extracellular-matrix (ECM) leading to pericyte and vascular endothelial cell damage that affects the retinal circulation. In turn, this causes hypoxia leading to release of vascular endothelial growth factor (VEGF) to induce the angiogenesis process. Alpha-1 antitrypsin (AAT) is the most important circulating inhibitor of serine proteases (SERPIN). Its targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, proteinase 3 (PR-3) and plasminogen activator (PAI). AAT modulates the effect of protease-activated receptors (PARs) during inflammatory responses. Plasma levels of AAT...
Tipo: Journal article
Palavras-chave: Diabetic retinopathy; Alpha-1-antitrypsin; Diabetes; Endogenous anti-inflammatory agents; Retinal inflammation; NF-kB.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100052

Regulation of Pax7 protein levels by caspase-3 and proteasome activity in differentiating myoblasts
Provedor de dados: 48
Autores: Olguín,Hugo C.
The transcription factor Pax7 negatively regulates the activity of the muscle regulatory transcription factor MyoD, preventing muscle precursor cells from undergoing terminal differentiation. In this context, the ratio between Pax7 and MyoD protein levels is thought to be critical in allowing myogenesis to proceed or to maintain the undifferentiated muscle precursor state. We have previously shown that Pax7 is subject to rapid down regulation in differentiating myoblasts, via a proteasome-dependent pathway. Here we present evidence indicating that Pax7 is also subject to caspase-3-dependent regulation. Furthermore, simultaneous inhibition of caspase-3 and proteasome activity induced further accumulation of Pax7 protein in differentiating myoblasts. These...
Tipo: Journal article
Palavras-chave: Pax7; Caspase-3; Calpains; Proteasome; Satellite cells; Muscle differentiation.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400002

Musk gland seasonal development and musk secretion are regulated by the testis in muskrat (Ondatra zibethicus)
Provedor de dados: 48
Autores: Zhang,Tianxiang; Peng,Dong; Qi,Lei; Li,Weixuan; Fan,Mengyuan; Shen,Jiachen; Yang,Liangliang; Wang,Yihua; Wang,Wenxia; Hu,Xiaolong; Cai,Ruibo; Zhou,Ran; Wei,Yuting; Zhou,Juntong; Yang,Shuang; Hu,Defu; Liu,Shuqiang.
Abstract  Background  The muskrat is a seasonal breeder. Males secrete musk to attract females during the breeding season. The testosterone binding to the androgen receptor (AR) in musk glands of muskrat may play an important role conducting the musk secretion process.  Methods  The musk gland, testis and blood samples of musk rats are collected in both breeding and non-breeding seasons. Some part of the samples are kept in liquid nitrogen for transcriptome analysis and Western blotting test. Some part of the samples are kept in 70% alcohol for histology experiment, blood samples are kept at &amp;#8722;20 °C for the serum testosterone measurement experiment.  Results  This study demonstrates that the quantity of secreted musk, the volume of the musk...
Tipo: Journal article
Palavras-chave: Muskrat; Musk gland; Testis; AR; Seasonal development.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100201

Genetic polymorphism in eight Chilean strains of the carotenogenic microalga Dunaliella salina Teodoresco (Chlorophyta)
Provedor de dados: 48
Autores: GOMEZ,PATRICIA I; GONZALEZ,MARIELA A.
Eight Chilean strains of Dunaliella salina obtained within a restricted geographic range, but exhibiting a high variability in their morphology, rate of growth and carotenogenic capacity, were analyzed by Random Amplified Polymorphic DNA (RAPD-PCR). Twenty of the 50 random primers (D, P, OPA and OPD series) that were tested amplified reproducible bands and were useful for comparative analysis of the strains. Of 107 polymorphic genetic markers, 49 were strain-specific. A great genetic variability was found among the strains in spite of their geographic proximity. In addition, phenetic analysis of the data showed close agreement between the morpho-physiological attributes and the genetic diversity of the strains
Tipo: Journal article
Palavras-chave: SS-carotene; Dunaliella salina; Genetic polymorphism; RAPD.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100012

Effects of isoflavone on oxidative stress parameters and homocysteine in postmenopausal women complaining of insomnia
Provedor de dados: 48
Autores: BRANDÃO,LETÍCIA C; HACHUL,HELENA; BITTENCOURT,LIA R; BARACAT,EDMUND C; TUFIK,SERGIO; D'ALMEIDA,VÂNIA.
Sleep disorders have an increased incidence after menopause. The objective of this work was to evaluate the effects of isoflavone on some oxidative stress markers in postmenopausal women complaining of insomnia. Women aged between 50-65 years (n=38) were recruited and assigned to a double-blind placebo controlled study for 4 months. The treated group received 100 mg/day of isoflavones. Blood collections were conducted on three different occasions to assess total glutathione; superoxide dismutase and catalase in erythrocytes; lipid peroxidation; and homocysteine plasma concentrations. No differences between the groups were observed. However, all the patients seem to improve their oxidative stress status and homocysteine concentration after treatment....
Tipo: Journal article
Palavras-chave: Homocysteine; Isoflavones; Insomnia; Menopause; Oxidative stress.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300002

Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
Provedor de dados: 48
Autores: WILHELM,VIVIAN; SOZA,CRISTIÁN; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D.T.
We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective...
Tipo: Journal article
Palavras-chave: Piscirickettsia salmonis; Hsp60; Hsp70; GroEL; DnaK; Immune response.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009

Gene expression profiling in wild-type and metallothionein mutant fibroblast cell lines
Provedor de dados: 48
Autores: ARMENDÁRIZ,ÁNGELA D; OLIVARES,FELIPE; PULGAR,RODRIGO; LOGUINOV,ALEX; CAMBIAZO,VERÓNICA; VULPE,CHRISTOPHER D; GONZÁLEZ,MAURICIO.
The role of metallothioneins (MT) in copper homeostasis is of great interest, as it appears to be partially responsible for the regulation of intracellular copper levels during adaptation to extracellular excess of the metal. To further investigate a possible role of MTs in copper metabolism, a genomics approach was utilized to evaluate the role of MT on gene expression. Microarray analysis was used to examine the effects of copper overload in fibroblast cells from normal and MT I and II double knock-out mice (MT-/-). As a first step, we compared genes that were significantly upregulated in wild-type and MT-/- cells exposed to copper. Even though wild-type and mutant cells are undistinguishable in terms of their morphological features and rates of growth,...
Tipo: Journal article
Palavras-chave: Copper homeostasis; Metallothionein; Microarray.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100015

A light- and electron microscopic study of primordial germ cells in the zebra fish (Danio rerio)
Provedor de dados: 48
Autores: Deniz Koç,Nazan; Yüce,Rikap.
In sexually reproducing organisms, primordial germ cells (PGCs) give rise to the cells of the germ line, the gametes. In many animals, PGCs are set apart from somatic cells early during embryogenesis. This study explores the origin of primordial germ cells (PGCs) of the zebra fish and examines their morphology during early development (1st day-15th day). PGCs were selectively stained by the alkaline phosphatase histochemical reaction and viewed by light and electron microscopy from the time they are first detectable in the yolk sac endoderm. PGCs occurred in the subendodermal space on the syncytial periblast; differing from the surrounding endodermal cells. Later the PGCs moved to between the blastoderm and yolk sac and transferred to the dorsal mesentery...
Tipo: Journal article
Palavras-chave: Primordial germ cell; Zebra fish; Ultrastructure; Morphology.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400001

In vivo analgesic, antipyretic, and anti-inflammatory potential in Swiss albino mice and in vitro thrombolytic activity of hydroalcoholic extract from Litsea glutinosaleaves
Provedor de dados: 48
Autores: Bhowmick,Rumpa; Sarwa,Md Shahid; Dewan,Syed Masudur Rahman; Das,Abhijit; Das,Binayok; Uddin,Mir Muhammad Nasir; Islam,Md Siddiqul; Islam,Mohammad Safiqul.
BACKGROUND: The study was conducted to evaluate the in vitro thrombolytic activity, and in vivo analgesic, anti-inflammatory and antipyretic potentials of different hydrocarbon soluble extracts of Litsea glutinosaleaves for the first time widely used in the folkloric treatments in Bangladesh. This work aimed to create new insights on the fundamental mechanisms of the plant extracts involved in these activities. RESULTS: In thrombolytic activity assay, a significant clot disruption was observed at dose of 1 mg/mL for each of the extracts (volume 100 &amp;#956;L) when compared to the standard drug streptokinase. The n-hexane, ethyl acetate, chloroform, and crude methanolic extracts showed 32.23&amp;#8201;±&amp;#8201;0.26, 37.67&amp;#8201;±&amp;#8201;1.31,...
Tipo: Journal article
Palavras-chave: Acetic acid induced writhing; Analgesic; Anti-inflammatory; Antipyretic; Litsea glutinosa; Thrombolytic.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100050

EL APORTE DE LUCO A LA NEUROCIENCIA CHILENA
Provedor de dados: 48
Autores: INESTROSA,NIBALDO C.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200003

Hemoglobin affinity for oxygen in three subspecies of toads (Bufo sp.) living at different altitudes
Provedor de dados: 48
Autores: OSTOJIC,HRVOJ; MONGE-C,CARLOS; CIFUENTES,VERONICA.
Blood oxygen affinity and red blood cell properties were measured in three subspecies of genus Bufo: Bufo spinulosus limensis, collected at sea level and at an average day temperature of 20ºC; Bufo spinulosus trifolium, from 3100 m, average day temperature of 15ºC; and Bufo spinulosus flavolineatus, from 4100 m, average day temperature of 10ºC. Electrophoresis of the hemoglobin showed the same component in each of the three subspecies. At 20ºC the blood oxygen affinities (P50) showed small differences between Bufo spinulosus limensis and Bufo spinulosus trifolium, whereas the value for Bufo spinulosus flavolineatus was markedly lower. At 10ºC, the ambient temperature of Bufo spinulosus flavolineatus, the P50 was extremely low compared with the other two...
Tipo: Journal article
Palavras-chave: Adaptations; Altitude; Amphibians; Bufo spinulosus limensis; Bufo spinulosus trifolium; Bufo spinulosus flavolineatus; Hemoglobin affinity; Hematocrits; Oxygen binding; Toads.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100007

The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae)
Provedor de dados: 48
Autores: Moresco,Rafaela M; Maniglia,Thiago C; De Oliveira,Classius; Margarido,Vladimir P.
The greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (Fst) proved to be high among the population of Marmeleiro-PR and the...
Tipo: Journal article
Palavras-chave: ESU; Gene flow; Genetic structure; Molecular marker.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100008

The action potential: From voltage-gated conductances to molecular structures
Provedor de dados: 48
Autores: BEZANILLA,FRANCISCO.
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300005

Curcumin restores mitochondrial functions and decreases lipid peroxidation in liver and kidneys of diabetic db/db mice
Provedor de dados: 48
Autores: Soto-Urquieta,María G; López-Briones,Sergio; Pérez-Vázquez,Victoriano; Saavedra-Molina,Alfredo; González-Hernández,Gloria A; Ramírez-Emiliano,Joel.
BACKGROUND: Nitrosative and oxidative stress play a key role in obesity and diabetes-related mitochondrial dysfunction. The objective was to investigate the effect of curcumin treatment on state 3 and 4 oxygen consumption, nitric oxide (NO) synthesis, ATPase activity and lipid oxidation in mitochondria isolated from liver and kidneys of diabetic db/db mice. RESULTS: Hyperglycaemia increased oxygen consumption and decreased NO synthesis in liver mitochondria isolated from diabetic mice relative to the control mice. In kidney mitochondria, hyperglycaemia increased state 3 oxygen consumption and thiobarbituric acid-reactive substances (TBARS) levels in diabetic mice relative to control mice. Interestingly, treating db/db mice with curcumin improved or...
Tipo: Journal article
Palavras-chave: Diabetes; Mitochondria; Curcumin; ATPase activity; Nitric oxide synthesis; Lipid oxidation.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100068

Sox2 function as a negative regulator to control HAMP expression
Provedor de dados: 48
Autores: Song,Bin; Bian,Qi; Shao,Cheng-Hao; Liu,An-An; Jing,Wei; Liu,Rui; Zhang,Yi-Jie; Zhou,Ying-Qi; Li,Gang; Jin,Gang.
BACKGROUND: Hepcidin, encoding by HAMP gene, is the pivotal regulator of iron metabolism, controlling the systemic absorption and transportation of irons from intracellular stores. Abnormal levels of HAMP expression alter plasma iron parameters and lead to iron metabolism disorders. Therefore,itis animportant goal to understand the mechanisms controlling HAMP gene expression. RESULTS: Overexpression of Sox2 decrease basal expression of HAMP or induced by IL-6 or BMP-2, whereas, knockdown of Sox2 can increase HAMP expression, furthermore, two potential Sox2-binding sites were identified within the human HAMP promoter. Indeed, luciferase experiments demonstrated that deletion of any Sox2-binding site impaired the negative regulation of Sox2 on HAMP promoter...
Tipo: Journal article
Palavras-chave: Sox2; Gene expression; Hepatocyte; HAMP; Iron metabolism.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100023

Risk of Dementia and Alcohol and Wine Consumption: a Review of Recent Results
Provedor de dados: 48
Autores: LETENNEUR,LUC.
The term dementia refers to a clinical syndrome of acquired intellectual disturbances produced by brain dysfunction. Dementia may result from a wide variety of disorders, including degenerative (e.g. Alzheimer's disease, AD), vascular (e.g. multi-infarct dementia), and traumatic (e.g. head injury). Long-term abuse of alcohol is related to the development of the Wernicke-Korsakoff's syndrome or alcohol dementia. However, light to moderate alcohol intake might also reduce the risk of dementia and AD. In Bordeaux (France), a population-based prospective study found that subjects drinking 3 to 4 standard glasses of wine per day (&gt; 250 and up to 500 ml), categorized as moderate drinkers, the crude odds ratio (OR) was 0.18 for incident dementia (p &lt; 0.01)...
Tipo: Journal article
Palavras-chave: Alcohol; Alzheimer's disease; Dementia; Wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200003

Comparison of Phenotypic and Genetic Performance of Local Silkworm Groups and Two Commercial Lines
Provedor de dados: 48
Autores: Mirhosseini,Seyed Ziaeddin; Nematollahian,Shahla; Ghanipoor,Mani; Seidavi,Alireza.
Five Iranian native silkworm groups: Baghdad, Khorasan Orange, Guilan Orange, Khorasan Pink, Khorasan Lemon, and 107 and 110 commercial lines (12 families from each breed) were randomly selected and reared during 2003-2005 (five generations in spring and autumn). In each family, 30 male and 30 female cocoons were individually recorded for weight, shell weight and shell ratio. From among the native groups, the highest average in all three traits belonged to Baghdad and Khorasan Pink, and the lowest to Khorasan Orange and Khorasan Lemon. From among the commercial lines, the highest average in all three traits belonged to 107. In comparing heritabihty for cocoon weight in native groups, the highest heritabihty belonged to Guilan Orange (0.5147) and Khorasan...
Tipo: Journal article
Palavras-chave: Native silkworm; Commercial lines; Bombyx mori L.; Heritabihty; Genetic correlation.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400005

Código de Ética de la Sociedad de Biología de Chile
Provedor de dados: 48
Tipo: Journal article
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300012

Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)
Provedor de dados: 48
Autores: AKINPELU,DAVID A; AIYEGORO,AYOBAMI O; OKOH,ANTHONY I.
We had recently reported antibacterial activity in the crude extract of the stem bark of Afzelia africana (Akinpelu et al., 2008). In this study, we assessed the biocidal and cell membrane disruption potentials of fractions obtained from the crude extract of the plant. The aqueous (AQ) and butanol (BL) fractions exhibited appreciable antibacterial activities against the test bacteria. The minimum inhibitory concentrations of the AQ and BL fractions ranged between 0.313 and 2.5 mg/ml, while their minimum bactericidal concentrations varied between 0.625 and 5.0 mg/ml. Also, the AQ fraction killed about 95.8% of E. coli cells within 105 min at a concentration of 5 mg/ml, while about 99.1% of Bacillus pumilus cells were killed by this fraction at the same...
Tipo: Journal article
Palavras-chave: Afzelia africana; Antibacteria; Phytochemistry; Killing rate; Protein leakage.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300008

Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
Provedor de dados: 48
Autores: Geng,Wenjia; Wei,Ribao; Liu,Shuwen; Tang,Li; Zhu,Hanyu; Chen,Pu; Wu,Jie; Zhang,Xueguang; Zhu,Fei; Yin,Zhong; Chen,Xiangmei.
BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: (1) Sham surgery (Sham); (2) anti-Thy-1 nephritis model (Thy-1); (3) anti-Thy-1 nephritis model + irbesartan-treated (Irb); (4) anti-Thy-1 nephritis model + low-dose of Shenhua Tablet (SHL); (5) anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet (SHM); (6) anti-Thy-1 nephritis model + high-dose of Shenhua Tablet (SHH RESULTS: Thirteen weeks after drug treatment, urinary proteins were quantified and renal pathological...
Tipo: Journal article
Palavras-chave: Shenhua Tablet; Chronic anti-Thy-1 glomerulonephritis; Mesangial cell proliferation; Erk.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100017

CoagVDb: a comprehensive database for coagulation factors and their associated SAPs
Provedor de dados: 48
Autores: Ali,Shabana Kouser; George Priya Doss,C; Thirumal Kumar,D; Zhu,Hailong.
The current state of the art in medical genetics is to identify and classify the functional (deleterious) or non-functional (neutral) single amino acid substitutions (SAPs), also known as non-synonymous SNPs (nsSNPs). The primary goal is to elucidate the mechanisms through which functional SAPs exert their effects, and ultimately interrogating this information for association with complex phenotypes. This work focuses on coagulation factors involved in the coagulation cascade pathway which plays a vital role in the maintenance of homeostasis in the human system. We developed an integrated coagulation variation database, CoagVDb, which makes use of the biological information from various public databases such as NCBI, OMIM, UniProt, PDB and SAPs...
Tipo: Journal article
Palavras-chave: Coagulation cascade pathway; Coagulation factor proteins; Single amino acid substitutions; SIFT; PolyPhen 2; I-Mutant 3; Fathmm; Align GVGD; PhD-SNP; SNPs&amp; GO; SNAP.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100035

Molecular and functional characterization of ferredoxin NADP(H) oxidoreductase from Gracilaria chilensis and its complex with ferredoxin
Provedor de dados: 48
Autores: Vorphal,María Alejandra; Bruna,Carola; Wandersleben,Traudy; Dagnino-Leone,Jorge; Lobos-González,Francisco; Uribe,Elena; Martínez-Oyanedel,José; Bunster,Marta.
Abstract  Backgroud Ferredoxin NADP(H) oxidoreductases (EC 1.18.1.2) (FNR) are flavoenzymes present in photosynthetic organisms; they are relevant for the production of reduced donors to redox reactions, i.e. in photosynthesis, the reduction of NADP+ to NADPH using the electrons provided by Ferredoxin (Fd), a small FeS soluble protein acceptor of electrons from PSI in chloroplasts. In rhodophyta no information about this system has been reported, this work is a contribution to the molecular and functional characterization of FNR from Gracilaria chilensis, also providing a structural analysis of the complex FNR/Fd.  Methods The biochemical and kinetic characterization of FNR was performed from the enzyme purified from phycobilisomes enriched fractions. The...
Tipo: Journal article
Palavras-chave: Ferredoxin NADP+ reductase; Ferredoxin; Sequence; Kinetic parameters; Structural features.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100228

Regulation of capacitative and non-capacitative Ca2+ entry in A7r5 vascular smooth muscle cells
Provedor de dados: 48
Autores: TAYLOR,COLIN W; MONEER,ZAHID.
A capacitative Ca2+ entry (CCE) pathway, activated by depletion of intracellular Ca2+ stores, is thought to mediate much of the Ca2+ entry evoked by receptors that stimulate phospholipase C (PLC). However, in A7r5 vascular smooth muscle cells, vasopressin, which stimulates PLC, empties intracellular Ca2+ stores but simultaneously inhibits their ability to activate CCE. The diacylglycerol produced with the IP3 that empties the stores is metabolized to arachidonic and this leads to activation of nitric oxide (NO) synthase, production of NO and cyclic GMP, and consequent activation of protein kinase G. The latter inhibits CCE. In parallel, NO directly activates a non-capacitative Ca2+ entry (NCCE) pathway, which is entirely responsible for the Ca2+ entry that...
Tipo: Journal article
Palavras-chave: Ca2+ entry; Smooth muscle; Archidonic acid; Nitric oxide; Cyclic GMP.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400020

BRCA1 and BRCA2mutations in breast cancer patients from Venezuela
Provedor de dados: 48
Autores: Lara,Karlena; Consigliere,Nigmet; Pérez,Jorge; Porco,Antonietta.
A sample of 58 familial breast cancer patients from Venezuela were screened for germline mutations in the coding sequences and exon-intron boundaries of BRCA1 (MIM no. 113705) and BRCA2 (MIM no. 600185) genes by using conformation-sensitive gel electrophoresis. Ashkenazi Jewish founder mutations were not found in any of the samples. We identified 6 (10.3%) and 4 (6.9%) patients carrying germline mutations in BRCA1 and BRCA2, respectively. Four pathogenic mutations were found in BRCA1, one is a novel mutation (c.951_952insA), while the other three had been previously reported (c.1129_1135insA, c.4603G&gt;T and IVS20+1G&gt;A). We also found 4 pathogenic mutations in BRCA2, two novel mutations (c.2732_2733insA and c.3870_3873delG) and two that have been...
Tipo: Journal article
Palavras-chave: BRCA genes; Breast cancer; Screening mutations.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200003

Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
Provedor de dados: 48
Autores: Urriola-Muñoz,Paulina; Lizama,Carlos; Lagos-Cabré,Raúl; Reyes,Juan G; Moreno,Ricardo D.
BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. RESULTS: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of...
Tipo: Journal article
Palavras-chave: Testis; Spermatogenesis; Apoptosis; Paracrine; Metalloproteinase.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100031

Soluble carbohydrate content variation in Sanionia uncinata and Polytrichastrum alpinum, two Antarctic mosses with contrasting desiccation capacities
Provedor de dados: 48
Autores: Zúñiga-González,Paz; Zúñiga,Gustavo E; Pizarro,Marisol; Casanova-Katny,Angélica.
BACKGROUND: Cryptogamic vegetation dominates the ice-free areas along the Antarctic Peninsula. The two mosses Sanionia uncinata and Polytrichastrum alpinum inhabit soils with contrasting water availability. Sanionia uncinata grows in soil with continuous water supply, while P. alpinum grows in sandy, non-flooded soils. Desiccation and rehydration experiments were carried out to test for differences in the rate of water loss and uptake, with non-structural carbohydrates analysed to test their role in these processes. RESULTS: Individual plants of S. uncinata lost water 60 % faster than P. alpinum; however, clumps of S. uncinata took longer to dry than those of P. alpinum (11 vs. 5 h, respectively). In contrast, rehydration took less than 10 min for both...
Tipo: Journal article
Palavras-chave: Antarctica; Antarctic vegetation; Bryophytes; Sugars.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100006

Amyloid-ß-peptide reduces copper(II) to copper(I) independent of its aggregation state
Provedor de dados: 48
Autores: OPAZO,CARLOS; RUIZ,FRANCISCA H; INESTROSA,NIBALDO C.
Alzheimer’s disease (AD) is characterized by the deposition of amyloid b-peptide (Aß) and neuronal degeneration in brain regions involved in learning and memory. One of the leading etiologic hypotheses regarding AD is the involvement of free radical-mediated oxidative stress in neuronal degeneration. Recent evidence suggests that metals concentrated in amyloid deposits may contribute to the oxidative insults observed in AD-affected brains. We hypothesized that Aß peptide in the presence of copper enhances its neurotoxicity generating free radicals via copper reduction. In the present study, we have examined the effect of the aggregation state of amyloid-ß-peptide on copper reduction. In independent experiments we measured the copper-reducing ability of...
Tipo: Journal article
Palavras-chave: Aß peptide; Amyloid fibrils; Copper reduction; Oxidative damage; Alzheimer’s Disease.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200012

Selected spices and their combination modulate hypercholesterolemia-induced oxidative stress in experimental rats
Provedor de dados: 48
Autores: Otunola,Gloria A; Oloyede,Oyelola B; Oladiji,Adenike T; Afolayan,Anthony J.
BACKGROUND: Effect of aqueous extracts of Allium sativum (garlic), Zingiber officinale (ginger), Capsicum fructensces (cayenne pepper) and their mixture on oxidative stress in rats fed high Cholesterol/high fat diet was investigated. Rats were randomly distributed into six groups (n&amp;#8201;=&amp;#8201;6) and given different dietary/spice treatments. Group 1 standard rat chow (control), group 2, hypercholesterolemic diet plus water, and groups 3, 4, 5, 6, hypercholesterolemic diet with 0.5 ml 200 mg&amp;#8201;·&amp;#8201;kg-1 aqueous extracts of garlic, ginger, cayenne pepper or their mixture respectively daily for 4 weeks. RESULTS: Pronounced oxidative stress in the hypercholesterolemic rats evidenced by significant (p&amp;#8201;&lt;&amp;#8201;0.05)...
Tipo: Journal article
Palavras-chave: Lipid peroxidation; Oxidative stress; Spices; Hypercholesterolemia; ROS.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100005

The acetyl xylan esterase II gene from Penicillium purpurogenum is differentially expressed in several carbon sources, and tightly regulated by pH
Provedor de dados: 48
Autores: CHÁVEZ,RENATO; SCHACHTER,KAREN; NAVARRO,CLAUDIO; PEIRANO,ALESSANDRA; BULL,PAULINA; EYZAGUIRRE,JAIME.
The expression of the acetyl xylan esterase II (axeII) gene from Penicillium purpurogenum is repressed by glucose and induced by xylan, as well as to a small degree by xylose and xylitol. This gene is expressed at neutral pH, but not under alkaline or acidic conditions, in agreement with previous findings for other xylanolytic genes of this organism. This is the first report showing pH regulation of an axe gene.
Tipo: Journal article
Palavras-chave: Acetyl xylan esterase; Gene expression; Penicillium purpurogenum.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100011

Pesticidal and pest repellency activities of a plant derived triterpenoid 2&amp;#945;,3&amp;#946;,21&amp;#946;,23,28-penta hydroxyl 12-oleanene against Tribolium castaneum
Provedor de dados: 48
Autores: Khan,Alam; Islam,Shariful; Rahman,Moizur; Zaman,Tanjeena; Haque,Ekramul.
BACKGROUND: Tribolium castaneum (Herbst) is a major pest of stored grain-based products, and cause severe damage to cereal grains throughout the world. The present investigation was aimed to determine the pesticidal and pest repellent activities of 2&amp;#945;,3&amp;#946;,21&amp;#946;,23,28-penta hydroxyl 12-oleanene against T. castaneum. The compound 2&amp;#945;,3&amp;#946;,21&amp;#946;,23,28-penta hydroxyl 12-oleanene is a triterpenoid which was isolated from the roots of Laportea crenulata Gaud. Surface film technique was used for pesticidal screening, whereas, pest repellency property of the triterpenoid was determined by filter paper disc method. RESULTS: At 24 hours of exposure duration, significant mortality records (80% and 86%) were observed at...
Tipo: Journal article
Palavras-chave: Laportea crenulata 2&amp;#945; 3&amp;#946; 21&amp;#946; 23 28-penta hydroxyl 12-oleanene Botanical pesticide Botanical pest repellent Tribolium castaneum.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100062

Saline-induced changes of epicuticular waxy layer on the Puccinellia tenuiflora and Oryza sativa leave surfaces
Provedor de dados: 48
Autores: Yang,Chunxue; Ma,Shurong; Lee,Imshik; Kim,Jaineung; Liu,Shenkui.
BACKGROUND: The epicuticular waxy layer of plant leaves enhances the extreme environmental stress tolerance. However, the relationship between waxy layer and saline tolerance was not established well. The epicuticular waxy layer of rice (Oryza sativa L.) was studied under the NaHCO3 stresses. In addition, strong saline tolerance Puccinellia tenuiflora was chosen for comparative studies. RESULTS: Scanning electron microscope (SEM) images showed that there were significant changes in waxy morphologies of the rice epicuticular surfaces, while no remarkable changes in those of P. tenuiflora epicuticular surfaces. The NaHCO3-induced morphological changes of the rice epicuticular surfaces appeared as enlarged silica cells, swollen corns-shapes and leaked salt...
Tipo: Journal article
Palavras-chave: Saline tolerance; Morphology; Wax crystal; Element analysis; Na+ localization.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100033

Expression of resistin, CXCR3, IP-10, CCR5 and MIP-1&amp;#945; in obese patients with different severity of asthma
Provedor de dados: 48
Autores: Rojas-Dotor,Sara; Segura-Méndez,Nora H; Miyagui-Namikawa,Ken; Mondragón-González,Rafael.
Asthma studies suggest that alteration in the inflammation pattern may be associated with the severity of asthma. The aim of this study was to compare in vitro the expression of chemokines, chemokine receptors and cytokine production from CD4+ T human lymphocytes of asthmatic, both obese and non-obese patients with different severity levels of asthma. Lymphocytes were labeled with monoclonal anti-human CXCR3/IP-10, MIP-1a/CCR5 antibodies and were analyzed by flow cytometry. Cell culture supernatants were used to measure production of interleukin IL-6 and resistin by ELISA. CXCR3/IP-10 expression increased in non-obese patients with mild persistent asthma (2.2%, p&lt;0.05), moderate persistent asthma (3%, p&lt;0.003) and severe persistent asthma (4%,...
Tipo: Journal article
Palavras-chave: Asthma classification severity chemokines (MIP-1a; IP-10) CC chemokine receptors (CXCR3; CCR5) interleukin-6 resistin.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100002

XXVI REUNIÓN ANUAL DE LA SOCIEDAD DE BIOQUÍMICA Y BIOLOGÍA MOLECULAR DE CHILE
Provedor de dados: 48
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300015

The Bacteriophage lambdaDNA packaging enzyme: Identification of four structural domains of the gpNu1 subunit using limited proteolysis
Provedor de dados: 48
Autores: ARAYA,PAMELA; ROSEMBLATT,MARIO; VALENZUELA,PABLO; MURIALDO,HELIOS.
Lambda DNA terminase, the enzyme that cleaves virion-length chromosomes from multigenomic concatemers and packages them into the bacteriophage head, is composed of two subunits, gpNu1 and gpA. Direct determination of the structure of gpNu1, the smaller subunit, has not been possible because of its insolubility in aqueous solutions. Therefore, to identify smaller and potentially water-soluble domains of gpNu1, we analyzed the nature of the products obtained by limited digestion of the protein with several proteases. The gpNu1 subunit was obtained from E.coli cells transfected with the plasmid pH6-Nu1 that overproduces the protein. Incubation of gpNu1 solubized in 2.5 M guanidinium chloride with chymotrypsin resulted in the formation of at least eight...
Tipo: Journal article
Palavras-chave: DNA packaging; Terminase; GpNu1 structural domains; Limited proteolysis.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300008

Characterization of Escherichia coli virulence genes, pathotypes and antibiotic resistance properties in diarrheic calves in Iran
Provedor de dados: 48
Autores: Shahrani,Masoud; Dehkordi,Farhad Safarpoor; Momtaz,Hassan.
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Tipo: Journal article
Palavras-chave: Antibiotic resistance profile; Diarrheic calves; Escherichia coli; Iran; Serogroups; Virulence genes.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100028

Protein synthesis in eukaryotes: The growing biological relevance of cap-independent translation initiation
Provedor de dados: 48
Autores: LÓPEZ-LASTRA,MARCELO; RIVAS,ANDREA; BARRÍA,MARÍA INÉS.
Ribosome recruitment to eukaryotic mRNAs is generally thought to occur by a scanning mechanism, whereby the 40S ribosomal subunit binds in the vicinity of the 5'cap structure of the mRNA and scans until an AUG codon is encountered in an appropriate sequence context. Study of the picornaviruses allowed the characterization of an alternative mechanism of translation initiation. Picornaviruses can initiate translation via an internal ribosome entry segment (IRES), an RNA structure that directly recruits the 40S ribosomal subunits in a cap and 5' end independent fashion. Since its discovery, the notion of IRESs has extended to a number of different virus families and cellular RNAs. This review summarizes features of both cap-dependent and IRES-dependent...
Tipo: Journal article
Palavras-chave: Protein synthesis; Translation initiation; Internal ribosome entry segment.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200003

Improving the editorial management: A new editorial panel
Provedor de dados: 48
Tipo: Journal article
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100001

IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
Provedor de dados: 48
Autores: JAIMOVICH,ENRIQUE; CARRASCO,MARIA ANGELICA.
Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including...
Tipo: Journal article
Palavras-chave: Excitation-transcription; Inositol trisphosphate; Mitogen-activated kinases; CREB; Early genes; Dihydropyridine receptors.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200010

CALCIUM RELEASE AND CELLULAR CALCIUM SIGNALLING DOMAINS
Provedor de dados: 48
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100012

Photosynthesis at the far-red region of the spectrum in Acaryochloris marina
Provedor de dados: 48
Autores: Badshah,Syed Lal; Mabkhot,Yahia; Al&amp;#8211;Showiman,Salim S.
Abstract Acaryochloris marina is an oxygenic cyanobacterium that utilizes far&amp;#8211;red light for photosynthesis. It has an expanded genome, which helps in its adaptability to the environment, where it can survive on low energy photons. Its major light absorbing pigment is chlorophyll d and it has &amp;#945;&amp;#8211;carotene as a major carotenoid. Light harvesting antenna includes the external phycobilin binding proteins, which are hexameric rods made of phycocyanin and allophycocyanins, while the small integral membrane bound chlorophyll binding proteins are also present. There is specific chlorophyll a molecule in both the reaction center of Photosystem I (PSI) and PSII, but majority of the reaction center consists of chlorophyll d. The composition...
Tipo: Journal article
Palavras-chave: Oxygenic photosynthesis; Acaryochloris species; Chlorophyll d; Photosystem; Phycobillisomes; P740.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100212

The effect of cigarette smoke on fertilization and pre-implantation development: assessment using animal models, clinical data, and stem cells
Provedor de dados: 48
Autores: Talbot,Prue; Lin,Sabrina.
Numerous studies have repeatedly shown that women who smoke experience problems establishing and maintaining pregnancies, and recent work has further demonstrated that the in utero effects of smoke may not be manifested until months or even years after birth. The purpose of this review is to examine the recent literature dealing with the effects of cigarette smoke on the earliest stages of human prenatal development. Studies in this area have included the use of animal models, patients undergoing in vitro fertilization, and embryonic stem cell models. Events leading to fertilization, such as cumulus expansion, hyperactivation of sperm motility, and oocyte pick-up by the oviduct are all impaired by smoke exposure in animal models. Steps crucial to...
Tipo: Journal article
Palavras-chave: Cigarette smoke; Embryos; Fertilization; IVF; Stem cells; Toxicology.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200011

Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)
Provedor de dados: 48
Autores: CAVIEDES,PABLO; CAVIEDES,RAÚL; RAPOPORT,STANLEY I.
Down syndrome is determined by the presence of an extra copy of autosome 21 and is expressed by multiple abnormalities, with mental retardation being the most striking feature. The condition results in altered electrical membrane properties of fetal dorsal root ganglia (DRG) neurons, as in the trisomy 16 fetal mouse, an animal model of the human condition. Cultured trisomic DRG neurons from human and mouse fetuses present faster rates of depolarization and repolarization in the action potential compared to normal controls and a shorter spike duration. Also, trisomy 16 brain and spinal cord tissue exhibit reduced acetylcholine secretion. Therefore, we decided to study Ca2+ currents in cultured DRG neurons from trisomy 16 and age-matched control mice, using...
Tipo: Journal article
Palavras-chave: Trisomy 16; Dorsal root ganglion; Calcium current; Tissue culture; Patch clamp; Down syndrome.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300009

Understanding copper homeostasis in humans and copper effects on health
Provedor de dados: 48
Autores: ARAYA,MAGDALENA; PIZARRO,FERNANDO; OLIVARES,MANUEL; ARREDONDO,MIGUEL; GONZÁLEZ,MAURICIO; MÉNDEZ,MARCO.
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100020

F(ab')2 antibody fragments against Trypanosoma cruzi calreticulin inhibit its interaction with the first component of human complement
Provedor de dados: 48
Autores: AGUILAR,LORENA; RAMÍREZ,GALIA; VALCK,CAROLINA; MOLINA,MARÍA C; ROJAS,ÁLVARO; SCHWAEBLE,WILHELM; FERREIRA,VIVIANA; FERREIRA,ARTURO.
Trypanosoma cruzi calreticulin (TcCRT), described in our laboratory, retains several important functional features from its vertebrate homologues. We have shown that recombinant TcCRT inhibits the human complement system when it binds to the collagenous portion of C1q. The generation of classical pathway convertases and membrane attack complexes is thus strongly inhibited. In most T. cruzi-infected individuals, TcCRT is immunogenic and mediates the generation of specific antibodies. By reverting the C1q / TcCRT interaction, a parasite immune evasion strategy, these antibodies contribute to the host / parasite equilibrium. In an in vitro correlate of this situation, we show that the C1q / TcCRT interaction is inhibited by F(ab')2 polyclonal anti-TcCRT IgG...
Tipo: Journal article
Palavras-chave: Calreticulin; C1q; F(ab')2 antibody fragments; Trypanosoma cruzi.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200008

Development of a novel method to evaluate sialylation of glycoproteins and analysis of gp96 sialylation in Hela, SW1990 and A549 cell lines
Provedor de dados: 48
Autores: Liang,Yangui; Hua,Qiang; Pan,Pengwei; Yang,Jie; Zhang,Qi.
BACKGROUND: Glycoproteins play a critical role in the cellular activities of eukaryotes. Sialic acid is typically the outermost monosaccharide of glycolipids and glycoproteins, and is necessary for normal development. RESULTS: A strategy based on avidin-biotin affinity was established to enrich sialylated glycoproteins from HeLa cervical carcinoma, SW1990 pancreatic adenocarcinoma, and A549 lung adenocarcinoma cells. Using HPLC-MS/MS, western blot, real-time PCR, and enzyme-linked immunosorbent assay, gp96 was identified in all three cell lines. No significant difference in the protein expression of gp96 was detected at the whole cell level, but the amount of bioti-nylated gp96 in SW1990 cells was 30-40 % lower than that in A549 and HeLa cells, and the...
Tipo: Journal article
Palavras-chave: Click chemistry; Sialic acid; Gp96; Sialyltransferase.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100052

EDITORIAL
Provedor de dados: 48
Autores: MONASTERIO OPAZO,OCTAVIO.
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000400001

Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth
Provedor de dados: 48
Autores: Karimi,Ehsan; Jaafar,Hawa ZE; Ghasemzadeh,Ali; Ebrahimi,Mahdi.
BACKGROUND: The present study was conducted in order to evaluate the fatty acid profile, anti-oxidant and anti-bacterial activities from the microwave aqueous extract of the leaves of three different varieties of Labisia pumila Benth. RESULTS: The chemical analysis of the extract showed that fatty acids (palmitic, palmitoleic, stearic, oleic, linoleic and &amp;#945;-linolenic) acid as the main components in three varieties of L. pumila leaves. Furthermore, the obtained results of the anti-oxidant revealed that L. pumila var. alata contained higher anti-oxidative activities compared to var. pumila and var. lanceolata. However, these values were lower than the tested anti-oxidant standards. On the other hand, the aqueous leaf extracts in all three varieties...
Tipo: Journal article
Palavras-chave: Fatty acid composition; Microwave aqueous extraction; Anti-oxidant activities; Anti-bacterial activities.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100008

Las empresas chilenas, la ciencia y la tecnología
Provedor de dados: 48
Autores: CÁCERES C.,CARLOS F.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100003

IL-17A levels increase in the infarcted region of the left ventricle in a rat model of myocardial infarction
Provedor de dados: 48
Autores: Ávalos,Ana María; Apablaza,Felipe A; Quiroz,Mariana; Toledo,Viviana; Peña,Juan Pedro; Michea,Luis; Irarrazabal,Carlos E; Carrión,Flavio A; Figueroa,Fernando E.
Th17 cells, a recently described subtype of CD4+ effector lymphocytes, have been linked to cell-mediated autoimmune and inflammatory diseases as well as to cardiovascular diseases. However, the participation of IL-17A in myocardial ischemic injury has not been clearly defined. We therefore conducted the present study to evaluate IL-17A and Th17-related cytokine levels in a rat model of myocardial infarction (MI). MI was induced in male Sprague Dawley rats by coronary artery ligation. Controls were sham-operated (Sh) or non-operated (C). Blood and samples from the left ventricle (LV) were collected at weeks 1 and 4 post-MI. At week 1, MI animals exhibited increased IL-6, IL-23 and TGF-&amp;#946; mRNA levels with no apparent change in IL-17 mRNA or protein...
Tipo: Journal article
Palavras-chave: Interleukin-17; Th17; Myocardial infarction; Cardiovascular disease.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200012

Olfactory or auditory stimulation and their hedonic valúes differentially modulate visual working memory
Provedor de dados: 48
Autores: DONOSO,ANA M; FAUNDES,VÍCTOR; FALCON,FELIPE; ESPARZA,PAULINA; MALDONADO,PEDRO E.
Working memory (WM) designates the retention of objects or events in conscious awareness when these are not present in the environment. Many studies have focused on the interference properties of distracter stimuli in working memory, but these studies have mainly examined the influence of the intensity of these stimuli. Little is known about the memory modulation of hedonic content of distracter stimuli as they also may affect WM performance or attentional tasks. In this paper, we have studied the performance of a visual WM task where subjects recollect from five to eight visually presented objects while they are simultaneously exposed to additional - albeit weak- auditory or olfactory distracter stimulus. We found that WM performance decreases as the...
Tipo: Journal article
Palavras-chave: Humans; Odor Hedonic; Visual Memory; Multimodal Memory.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400003

What we see is how we are: New paradigms in visual research
Provedor de dados: 48
Autores: MALDONADO A,PEDRO.
As most sensory modalities, the visual system needs to deal with very fast changes in the environment. Instead of processing all sensory stimuli, the brain is able to construct a perceptual experience by combining selected sensory input with an ongoing internal activity. Thus, the study of visual perception needs to be approached by examining not only the physical properties of stimuli, but also the brain's ongoing dynamical states onto which these perturbations are imposed. At least three different models account for this internal dynamics. One model is based on cardinal cells where the activity of few cells by itself constitutes the neuronal correlate of perception, while a second model is based on a population coding that states that the neuronal...
Tipo: Journal article
Palavras-chave: Perception; Synchrony; Neural coding.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500006

In vivo and in vitro estrogenic and progestagenic actions of Tibolone
Provedor de dados: 48
Autores: SADARANGANI,ANIL; SALGADO,ANA MARÍA; KATO,SUMIE; PINTO,MAURICIO; CARVAJAL,ANDRÉS; MONSO,CAROLINA; OWEN,GARETH I; VIGIL,PILAR.
Estrogen and progestin combination in hormone replacement therapy (HRT) increases the incidence of breast cancer, but decreases the endometrial cancer risk of unopposed estrogen. Therefore, a SERM such as Tibolone, that delivers the beneficial, but not the adverse side effects, of steroid hormones would be clinically advantageous. However, data from the Million Women Study suggests that Tibolone increases the risk of both breast and endometrial cancer. Herein, we assessed the estrogenic and progestagenic actions of Tibolone using transvaginal sonography studies and an in vitro model of breast (ZR-75, MCF7) and endometrial cancer (Ishikawa). The known cancer associated proteins (ER, EGFR, STAT5, tissue factor and Bcl-xL) were selected for study....
Tipo: Journal article
Palavras-chave: Breast/endometrium cancer estrogenic HRT progestagenic &amp;amp; Tibolone.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200014

Hepatoprotective effect of Cinnamon extracts against carbon tetrachloride induced oxidative stress and liver injury in rats
Provedor de dados: 48
Autores: MOSELHY,SAID S; ALI,HUSEIN K. H.
Cinnamon is used to flavor most foods in Arabian countries. The aim of this study was to evaluate the medicinal importance, reflecting an important trend in research. The hepatoprotective activity of aqueous and ethanolic extracts of cinnamon was investigated against carbon tetrachloride (CC1(4)) induced lipid peroxidation and hepatic injury in rats. The elevated serum AST and ALT enzymatic activities induced by CC1(4) were significantly restored to near normal by oral administration of 200 mg/kg of either extracts once daily for 7 days, as compared to untreated rats. There was a significant elevation in the level of liver malondialdhyde (MDA), while the activities of antioxidant enzymes superoxide dismutase and catalase (SOD and CAT) were significantly...
Tipo: Journal article
Palavras-chave: Cinnamon extracts; CCL; Oxidative stress; Rats.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100009

Integrin-&amp;#946;1, not integrin-&amp;#946;5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
Provedor de dados: 48
Autores: Zeng,Qiangcheng; Guo,Yong; Liu,Yongming; Li,Ruixin; Zhang,Xinchang; Liu,Lu; Wang,Yang; Zhang,Xizheng; Zou,Xianqiong.
BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-&amp;#946; (&amp;#946;1, &amp;#946;5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-&amp;#946; in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-&amp;#946;1 siRNA or integrin-&amp;#946;5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µ&amp;#949;) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted...
Tipo: Journal article
Palavras-chave: Tensile strain; Osteoblast; Extracellular matrix; Integrin; SiRNA.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025

Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves
Provedor de dados: 48
Autores: Sebei,Khaled; Sakouhi,Fawzi; Herchi,Wahid; Khouja,Mohamed Larbi; Boukhchina,Sadok.
BACKGROUND: In this paper, we have studied the essential oils chemical composition of the leaves of seven Eucalyptus species developed in Tunisia. Eucalyptus leaves were picked from trees growing in different arboretums in Tunisia. Choucha and Mrifeg arboretums located in Sedjnene, region of Bizerte (Choucha: E. maideni, E. astrengens et E. cinerea; Mrifeg : E. leucoxylon), Korbous arboretums located in the region of Nabeul, North East Tunisia with sub-humid bioclimate, (E. lehmani), Souiniet-Ain Drahem arboretum located in region of Jendouba (E. sideroxylon, E. bicostata). Essential oils were individually tested against a large panel of microorganisms includingStaphylococcus aureus (ATCC 6539), Escherichia coli (ATCC 25922), Enterococcus faecalis...
Tipo: Journal article
Palavras-chave: Eucalyptus; Essential oil; Antibacterial activity; -pinene.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100007

Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
Provedor de dados: 48
Autores: Yan,Yizhong; Li,Jianjun; Zhang,Yiya; Peng,Xiaozhen; Guo,Tianyao; Wang,Jirong; Hu,Weijun; Duan,Zhigui; Wang,Xianchun.
BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block...
Tipo: Journal article
Palavras-chave: Black widow spider (L. tredecimguttatus); Protein composition; Egg; Molecular mechanism; Property analysis; Toxicity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017

Electrophysiological properties of rat nodose ganglion neurons co-transplanted with carotid bodies into the chick chorioallantoic membrane
Provedor de dados: 48
Autores: Eugenín,Jaime; Eyzaguirre,Carlos.
The electrophysiological properties of nodose ganglion neurons were evaluated immediately after removing nodose ganglia from young adult rats and 3 to 10 days after nodose ganglia implantation _either alone or co-implanted with carotid bodies_ onto the chick chorioallantoic membrane. Implanted and co-implanted nodose neurons were less excitable than acutely recorded nodose neurons. Co-implanted neurons also showed reduced amplitudes for both action potentials and spike after-hyperpolarizations relative to those found in acutely recorded nodose ganglion neurons and a smaller time constant (&amp;#964;) than that found in implanted neurons. In addition, no spontaneous activity was recorded from nodose ganglion neurons co-implanted with carotid bodies during...
Tipo: Journal article
Palavras-chave: Arterial chemoreceptors; Synaptogenesis; Transplantation; Trophism.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400003

Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria
Provedor de dados: 48
Autores: POIATTI,VERA A D; DALMAS,FERNANDO R; ASTARITA,LEANDRO V.
The natural resistance of plants to disease is based not only on preformed mechanisms, but also on induced mechanisms. The defense mechanisms present in resistant plants may also be found in susceptible ones. This study attempted to analyze the metabolic alterations in plants of the potato Solanum tuberosum L. cv. Agata that were inoculated with the incompatible plant-pathogenic bacteria X. axonopodis and R. solanacearum, and the compatible bacterium E. carotovora. Levels of total phenolic compounds, including the flavonoid group, and the activities of polyphenol oxidase (PPO) and peroxidase (POX) were evaluated. Bacteria compatibility was evaluated by means of infiltration of tubers. The defense response was evaluated in the leaves of the potato plants....
Tipo: Journal article
Palavras-chave: Flavonoids; Hypersensitive response; Peroxidases; Phenolic compounds.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200009

The Role of Tyrosine 207 in the Reaction Catalyzed by Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase
Provedor de dados: 48
Autores: Andrade,Cherie; Sepulveda,Carolina; Cardemil,Emilio; Jabalquinto,Ana M.
The functional signifcance of tyrosine 207 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase was explored by examining the kinetic properties of the Tyr207Leu mutant. The variant enzyme retained the structural characteristics of the wild-type protein as indicated by circular dichroism, intrinsic fuorescence spectroscopy, and gel-exclusion chromatography. Kinetic analyses of the mutated variant showed a 15-fold increase in Km CO2, a 32fold decrease in Vmax, and a 6-fold decrease in Km for phosphoenolpyruvate. These results suggest that the hydroxyl group of Tyr 207 may polarize CO2 and oxaloacetate, thus facilitating the carboxylation/decarboxylation steps.
Tipo: Journal article
Palavras-chave: Phosphoenolpyruvate carboxykinase; Saccharomyces cerevisiae; CO2 interaction.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200007

Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
Provedor de dados: 48
Autores: LANZA,HENRIQUE; AFONSO-CARDOSO,SANDRA R; SILVA,ADRIANO G; NAPOLITANO,DANIELLE R; ESPÍNDOLA,FOUED S; PENA,JANETHE D.O; SOUZA,MARIA A.
Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine...
Tipo: Journal article
Palavras-chave: Calcium; Leishmania major; Macrophage; Magnesium; Nitric oxide.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004

Effect of carbamazepine and gabapentin on excitability in the trigeminal subnucleus caudalis of neonatal rats using a voltage-sensitive dye imaging technique
Provedor de dados: 48
Autores: Matsumoto,Akiko; Arisaka,Hirofumi; Hosokawa,Yuki; Sakuraba,Shigeki; Sugita,Takeo; Umezawa,Nobuo; Kaku,Yuki; Yoshida,Kazu-ichi; Kuwana,Shun-ichi.
BACKGROUND: The antiepileptic drugs carbamazepine and gabapentin are effective in treating neuropathic pain and trigeminal neuralgia. In the present study, to analyze the effects of carbamazepine and gabapentin on neuronal excitation in the spinal trigeminal subnucleus caudalis (Sp5c) in the medulla oblongata, we recorded temporal changes in nociceptive afferent activity in the Sp5c of trigeminal nerve-attached brainstem slices of neonatal rats using a voltage-sensitive dye imaging technique. RESULTS: Electrical stimulation of the trigeminal nerve rootlet evoked changes in the fluorescence intensity of dye in the Sp5c. The optical signals were composed of two phases, a fast component with a sharp peak followed by a long-lasting component with a period of...
Tipo: Journal article
Palavras-chave: Carbamazepine; Gabapentin; Rat brainstem; Spinal trigeminal nucleus; Voltage-sensitive dye imaging.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100036

Evolution of probability measures by cellular automata on algebraic topological Markov chains
Provedor de dados: 48
Autores: MAASS,ALEJANDRO; MARTÍNEZ,SERVET.
In this paper we review some recent results on the evolution of probability measures under cellular automata acting on a fullshift. In particular we discuss the crucial role of the attractiveness of maximal measures. We enlarge the context of the results of a previous study of topological Markov chains that are Abelian groups; the shift map is an automorphism of this group. This is carried out by studying the dynamics of Markov measures by a particular additive cellular automata. Many of these topics were within the focus of Francisco Varela's mathematical interests.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100010

Lipids, proteins, phenolic composition, antioxidant and antibacterial activities of seeds of peanuts (Arachis hypogaea l) cultivated in Tunisia
Provedor de dados: 48
Autores: Sebei,Khaled; Gnouma,Asma; Herchi,Wahid; Sakouhi,Faouzi; Boukhchina,Sadok.
Fatty acid composition of peanut seed oil in four varieties cultivated in Tunisia showed that linoleic (C18:2), oleic (C18:1) and palmitic (C16) acids account for more than 84% for Chounfakhi and Massriya and for more than 85% of the total fatty acids of Trabilsia and Sinya seed oil respectively. Seed oil contents were significantly different (P &amp;#8804; 0.05) and did not exceed 48%. The study of total phenolics revealed that Chounfakhi contained more total phenolics (2.1 mg GAE/g DW), followed by the Massriya and Sinya cultivars (1.35 mg GAE/g DW for each); Trabilsia presented the lowest total phenolic content with 1 mg GAE/g DW. Considerable antiradical ability was found, especially in the Trabilsia peanut seed cultivar (IC50 = 1550 &amp;#956;g/ml),...
Tipo: Journal article
Palavras-chave: Arachis hypogea; Oil; Sterols; Phenols; Antioxidant activity; Antibacterial activity.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300006

Effect of heparin on in vitro capacitation of boar sperm
Provedor de dados: 48
Autores: DAPINO,DORA G; MARINI,PATRICIA E; CABADA,MARCELO O.
Chlortetracycline (CTC) fluorescent pattern, the ability to undergo acrosome reaction (AR) upon exposure to 10 µM calcium ionophore A23187 and vitality estimation were used to investigate the effect of the sulfated glycosaminoglycan heparin on the in vitro capacitation of porcine spermatozoa. Sperm incubation in capacitating medium (CM) supplemented with 10 mM heparin for up to 120 min, showed an increase in the number of capacitated sperm (B pattern) and acrosome reacted sperm (AR pattern), without affecting their viability. In this condition, spermatozoa were incubated in CM depleted of albumin, calcium, bicarbonate or combinations, in the presence of heparin. In either calcium or bicarbonate-free media, capacitation was only basal and did not show...
Tipo: Journal article
Palavras-chave: Boar sperm; Capacitation; Heparin; Sulfated glycosaminoglycans.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500006

IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein
Provedor de dados: 48
Autores: ORTEGA,XIMENA; VELÁSQUEZ,JUAN CARLOS; PÉREZ,LUZ M.
IP3 increase and de novo synthesis of scoparone are produced in the hypersensitive response (HR) of lemon seedlings against the fungus Alternaria alternata. To elucidate whether a G-protein and/or a protein tyrosine kinase (PTK) are involved in signal transduction leading to the production of such a defensive response, we studied the HR in this plant system after treatment with G-protein activators alone and PTK inhibitors in the presence of fungal conidia. No changes in the level of IP3 were detected in response to the treatment with the G-protein activators cholera toxin or mastoparan, although the HR was observed in response to these compounds as determined by the scoparone synthesis. On the contrary, the PTK inhibitors lavendustin A and 2,5-dihidroxy...
Tipo: Journal article
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100011

SOBRE EL PREMIO NACIONAL DE CIENCIAS NATURALES
Provedor de dados: 48
Autores: Jaksic,Fabian.
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500001

The expression of extracellular fungal cell wall hydrolytic enzymes in different Trichoderma harzianum isolates correlates with their ability to control Pyrenochaeta lycopersici
Provedor de dados: 48
Autores: PÉREZ,LUZ MARÍA; BESOAÍN,XIMENA; REYES,MAURICIO; PARDO,GONZALO; MONTEALEGRE,JAIME.
Four isolates of Trichoderma harzianum (ThN3, Th11, Th12 and Th16) were selected for their ability to control the in vitro development of the tomato root pathogen Pyrenochaeta lycopersici. Analysis of the mechanisms involved in biocontrol showed that the formation of non-volatile metabolites appears to be one of those involved in biocontrol of P. lycopersici by all T. harzianum isolates tested. Nevertheless, the higher secretion of chitinases, both in number of isoenzymes and activity by the Th11 strain, correlated well with its higher ability to control this agent in laboratory and greenhouse experiments as compared to the other T. harzianum isolates tested. The secretion of ß -1,3-endoglucanases and/or proteases appeared to have less significance than...
Tipo: Journal article
Palavras-chave: Biological control; Corky root; Tomato root diseases.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300014

Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
Provedor de dados: 48
Autores: Takeyama,Masayuki; Yoneda,Masahiko; Gosho,Masahiko; Iwaki,Masayoshi; Zako,Masahiro.
BACKGROUND: Previous reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To test this hypothesis, we evaluated the temperature dependence of the expression of vascular endothelial growth factor-A (VEGF-A) and in vitro angiogen-esis in retinal pigment epithelium (RPE). RESULTS: We cultured ARPE-19 cells at 37, 35, 33 and 31°C and measured the expression of VEGF-A, VEGF-A splicing variants, and pigment epithelium-derived factor (PEDF). We performed an in vitro tube formation assay. The dehydrogenase activity was also evaluated at each...
Tipo: Journal article
Palavras-chave: Age-related macular degeneration (AMD); Hypothermia; Pigment epithelium-derived factor (PEDF); Retinal pigment epithelium (RPE); Vascular endothelial growth factor (VEGF); Vitrectomy.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100042

Testing of a new prototype surgical stapler that automates the rollover sleeve technique for venous anastomoses
Provedor de dados: 48
Autores: CARROLL,PATRICIA B; DEFARIA,WERVISTON; GANDIA,CARLOS; BERHO,MARIANA; MISIAKOS,EVANGELOS; TZAKIS,ANDREAS G.
The creation of successful vascular anastomoses is of primary importance in many surgical fields. Numerous attempts to automate this process have been made. These techniques have slowly gained acceptance, but their use is still limited. This report details feasibility testing of a new prototype stapler that automates the rollover sleeve technique for venous vascular anastomoses. Male and female mongrel dogs (n=7) (25-32 kg) were used. A segment of the right (n=5) or left (n=2) iliac vein was harvested for interposition grafts after the contra lateral side was transected. In each dog, two end-to-end venous anastomoses at the interposition grafts were performed. The standard anastomosis employed continuous mattress sutures. The experimental anastomosis was...
Tipo: Journal article
Palavras-chave: Automated stapler prototype testing; End-to-end venous anastomosis; Rollover sleeve technique; Surgical staplers; Vascular anastomoses.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300008

The expression of heterologous MAM-7 in Lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen Vibrio parahaemolyticus in vitro
Provedor de dados: 48
Autores: Beltran,Sebastian; Munoz-Bergmann,Cristian A; Elola-Lopez,Ana; Quintana,Javiera; Segovia,Cristopher; Trombert,Annette N.
BACKGROUND: Vibrio parahaemolyticus (V. parahaemolyticus) is a Gram-negative, halophilic bacterium recognized as one of the most important foodborne pathogen. When ingested, V. parahaemolyticus causes a self-limiting illness (Vibriosis), characterized mainly by watery diarrhoea. Treatment is usually oral rehydration and/or antibiotics in complicated cases. Since 1996, the pathogenic and pandemic V. parahaemolyticus O3:K6 serotype has spread worldwide, increasing the reported number of vibriosis cases. Thus, the design of new strategies for pathogen control and illness prevention is necessary. Lactobacillus sp. grouped Gram positive innocuous bacteria, part of normal intestinal microbiota and usually used as oral vaccines for several diarrheic diseases....
Tipo: Journal article
Palavras-chave: Lactobacillus; V. parahaemolyticus; Adhesion; Recombinants probiotics.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100002

Chronic Chagas disease: PCR-xenodiagnosis without previous microscopic observation is a useful tool to detect viable Trypanosoma cruzi
Provedor de dados: 48
Autores: Saavedra,Miguel; Zulantay,Inés; Apt,Werner; Martínez,Gabriela; Rojas,Antonio; Rodríguez,Jorge.
We evaluate the elimination of the microscopic stage of conventional xenodiagnosis (XD) to optimize the parasitological diagnosis of Trypanosoma cruzi in chronic Chagas disease. To this purpose we applied under informed consent two XD cages to 150 Chilean chronic chagasic patients. The fecal samples (FS) of the triatomines at 30, 60 and 90 days post feeding were divided into two parts: in one a microscopic search for mobile trypomastigote and/or epimastigote forms was performed. In the other part, DNA extraction-purification for PCR directed to the conserved region of kDNA minicircles of trypanosomes (PCR-XD), without previous microscopic observation was done. An XD was considered positive when at least one mobile T. cruzi parasite in any one of three...
Tipo: Journal article
Palavras-chave: Chronic Chagas disease; PCR; Triatoma infestans; Trypanosoma cruzi; Xenodiagnosis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300011

Color vision in the comb frequency domain
Provedor de dados: 48
Autores: BONNARDEL,VALÈRIE; VARELA,FRANCISCO J.
In 1982, Horace Barlow considered the question of human trichromacy in the context of information theory: according to the Sampling Theorem, three types of receptors covering the visible spectrum (400- -700 nm) might be sufficient to reconstruct the color signal. Although Barlow was led to reject the direct application of the Sampling Theorem to explain color dimensionality, the theoretical framework offers a fresh point of view for analyzing the color system in conjunction with the physical characteristics of natural color signals. This review aims to illustrate that if the strict mathematical reconstruction (as implied by the Sampling Theorem) is replaced by a pragmatic approximation of color signals, then trichromacy, with its subsequent opponent-color...
Tipo: Journal article
Palavras-chave: Color vision color dimensionality; Comb-filtered spectra color constancy Fourier analysis.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100011

Nanoinformatics: an emerging area of information technology at the intersection of bioinformatics, computational chemistry and nanobiotechnology
Provedor de dados: 48
Autores: González-Nilo,Fernando; Pérez-Acle,Tomás; Guínez-Molinos,Sergio; Geraldo,Daniela A; Sandoval,Claudia; Yévenes,Alejandro; Santos,Leonardo S; Laurie,V. Felipe; Mendoza,Hegaly; Cachau,Raúl E.
After the progress made during the genomics era, bioinformatics was tasked with supporting the flow of information generated by nanobiotechnology efforts. This challenge requires adapting classical bioinformatic and computational chemistry tools to store, standardize, analyze, and visualize nanobiotechnological information. Thus, old and new bioinformatic and computational chemistry tools have been merged into a new sub-discipline: nanoinformatics. This review takes a second look at the development of this new and exciting area as seen from the perspective of the evolution of nanobiotechnology applied to the life sciences. The knowledge obtained at the nano-scale level implies answers to new questions and the development of new concepts in different...
Tipo: Journal article
Palavras-chave: Bioinformatics; Molecular simulation; Nanobiotechnology; Nanoinformatics; Nanomedicine.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100006

Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
Provedor de dados: 48
Autores: SOUZA-JÚNIOR,S A; BECKER,T C A; CASTRO-PRADO,M A A.
Mutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of asexual recombinants (parameiotic segregants). The mutant uvsH, named B511, showed normal frequency of meiotic recombination in sexual crosses and high frequency of parameiotic segregants in the parasexual crossings with master strains (B511//A757 and B511//A288). Asexual haploid recombinants (parameiotic segregants), diploid and aneuploid segregants were recovered directly from the uvs//uvs+ heterokaryons (B511//A757 and B511// A288). Parameiotic segregants...
Tipo: Journal article
Palavras-chave: Mitotic crossing-over; Parameiosis; Uvs mutation.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100007

Biochemical characterization and inhibitory effects of dinophysistoxin-1, okadaic acid and microcystine l-r on protein phosphatase 2a purified from the mussel Mytilus chilensis.
Provedor de dados: 48
Autores: RIVAS,MARIELLA; GARCÍA,CARLOS; LIBERONA,JOSÉ L; LAGOS,NÉSTOR.
Protein phosphatases are involved in many cellular processes. One of the most abundant and best studied members of this class is protein phosphatase type-2A (PP2A). In this study, PP2A was purified from the mussel Mytilus chilensis. Using both SDS-PAGE and size exclusion gel filtration under denaturant conditions, it was confirmed that the PP2A fraction was essentially pure. The isolated enzyme is a heterodimer and the molecular estimated masses of the subunits are 62 and 28 kDa. The isolated PP2A fraction has a notably high p-NPP phosphatase activity, which is inhibited by NaCl. The hydrolytic p-NPP phosphatase activity is independent of the MgCl2 concentration. The time courses of the inhibition of the PP2A activity of p-NPP hydrolysis by increasing...
Tipo: Journal article
Palavras-chave: Chile; Diarrhetic Shellfish Poisoning (DSP); Mussel; Mytilus chilensis; Protein Phosphatase 2A; Microcystine L-R.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300005

A walk with Francisco Varela from first- to second-generation networks: In search of the structure, dynamics and metadynamics of an organism-centered immune system
Provedor de dados: 48
Autores: COUTINHO,ANTÓNIO.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100004

Antioxidant flavonols from fruits, vegetables and beverages: measurements and bioavailability
Provedor de dados: 48
Autores: CROZIER,ALAN; BURNS,JENNIFER; AZIZ,AZLINA A; STEWART,AMANDA J; RABIASZ,HELENA S; JENKINS,GARETH I; EDWARDS,CHRISTINE A; LEAN,MICHAEL EJ.
Flavonols are polyphenolic secondary plant metabolites that are present in varying levels in commonly consumed fruits, vegetables and beverages. Flavonols have long held an interest for nutritionists, which has increased following a Dutch study in the early 1990’s showing that dietary intake of flavonols was inversely correlated with the incidence of coronary heart disease. The main factors that have hindered workers in the field of flavonol research are (i) the accurate measurement of these compounds in foods and biological samples, and (ii) a dearth of information on their absorption and metabolism. This review aims to highlight the work of the authors in attempting to clarify the situation. The sensitive and selective HPLC procedure to identify and...
Tipo: Journal article
Palavras-chave: Bioavailability; Dietary antioxidants; Flavones; Flavonols; HPLC analysis.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200007

Letters to Editor / Erratum
Provedor de dados: 48
Autores: Loyola,Raúl EB.
Tipo: Journal article
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300014

The structure of selective dinucleotide interactions and periodicities in D melanogaster mtDNA
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
BACKGROUND: We found a strong selective 3-sites periodicity of deviations from randomness of the dinucleotide (DN) distribution, where both bases of DN were separated by 1, 2, K sites in prokaryotes and mtDNA. Three main aspects are studied. I) the specific 3 K-sites periodic structure of the 16 DN. II) to discard the possibility that the periodicity was produced by the highly nonrandom interactive association of contiguous bases, by studying the interaction of non-contiguous bases, the first one chosen each I sites and the second chosen J sites downstream. III) the difference between this selective periodicity of association (distance to randomness) of the four bases with the described fixed periodicities of base sequences. RESULTS: I) The 16 pairs...
Tipo: Journal article
Palavras-chave: Dinucleotides; DNA periodicity; Periodicity structure; Selective interactions; Theories of evolution.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100018

Synthetic Biology: opportunities for Chilean bioindustry and education
Provedor de dados: 48
Autores: Federici,Fernán; Rudge,Timothy J; Pollak,Bernardo; Haseloff,Jim; Gutiérrez,Rodrigo A.
In an age of pressing challenges for sustainable production of energy and food, the new field of Synthetic Biology has emerged as a promising approach to engineer biological systems. Synthetic Biology is formulating the design principles to engineer affordable, scalable, predictable and robust functions in biological systems. In addition to efficient transfer of evolved traits from one organism to another, Synthetic Biology offers a new and radical approach to bottom-up engineering of sensors, actuators, dynamical controllers and the biological chassis they are embedded in. Because it abstracts much of the mechanistic details underlying biological component behavior, Synthetic Biology methods and resources can be readily used by interdisciplinary teams to...
Tipo: Journal article
Palavras-chave: Synthetic biology; Bioengineering; Education; Engineering; Biotechnology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400010

Identification of novel gene and pathway targets for human epilepsy treatment
Provedor de dados: 48
Autores: Jin,Ying; Zhao,Chunzhe; Chen,Lihui; Liu,Xiangyu; Pan,Shuxiao; Ju,Dongsheng; Ma,Jing; Li,Jinying; Wei,Bo.
BACKGROUND: The aim of this study was to explore epilepsy-related mechanism so as to figure out the possible targets for epilepsy treatment. METHODS: The gene expression profile dataset GES32534 was downloaded from Gene Expression Omnibus database. We identified the differentially expressed genes (DEGs) by Affy package. Then the DEGs were used to perform gene ontology (GO) and pathway enrichment analyses. Furthermore, a protein-protein interaction (PPI) network was constructed with the DEGs followed by co-expression modules construction and analysis. RESULTS: Total 420 DEGs were screened out, including 214 up-regulated and 206 down-regulated genes. Functional enrichment analysis revealed that down-regulated genes were mainly involved in the process of...
Tipo: Journal article
Palavras-chave: Epilepsy; Differentially expressed genes; Functional annotation; Protein-protein interaction network; Co-expression module.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100003

Signal transduction and gene expression regulated by calcium release from internal stores in excitable cells
Provedor de dados: 48
Autores: CARRASCO,MARÍA ANGÉLICA; JAIMOVICH,ENRIQUE; KEMMERLING,ULRIKE; HIDALGO,CECILIA.
Calcium regulation of several transcription factors involves different calcium-dependent signaling cascades and engages cytoplasmic as well as nuclear calcium signals. The study of the specific sources of calcium signals involved in regulation of gene expression in skeletal muscle has been addressed only recently. In this tissue, most cytoplasmic and nuclear calcium signals originate from calcium release from internal stores, mediated either by ryanodine receptor (RyR) or IP3 receptor (IP3R) channels. The latter are located both in the sarcoplasmic reticulum (SR) and in the nuclear membrane, and their activation results in long-lasting nuclear calcium increase. The calcium signals mediated by RyR and IP3R are very different in kinetics, amplitude and...
Tipo: Journal article
Palavras-chave: Calcium signals; Intracellular stores; Transcription; Oxidation; Neurons; Skeletal muscle.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400028

Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
Provedor de dados: 48
Autores: JAIMOVICH,ENRIQUE; ESPINOSA,ALEJANDRA.
We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (&lt;1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The...
Tipo: Journal article
Palavras-chave: Skeletal; Transcription factors; Insulin; IGF-I; Testosterone.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018

Preventive effect of Ligularia fischeri on inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages depending on cooking method
Provedor de dados: 48
Autores: Park,Hee-Sook; Choi,Hye-Young; Kim,Gun-Hee.
BACKGROUND: Ligularia fischeri (common name Gomchwi) is known for its pharmaceutical properties and used in the treatment of jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases; however, little is known about its anti-inflammatory effect. In this study the influence of blanching and pan-frying on the anti-inflammatory activity of Ligularia fischeri (LF) was evaluated. RESULTS: Fresh LF and cooked LF showed no significant effect on the viability of macrophages after 24 h incubation. Fresh LF was found to be the most potent inhibitor of nitric oxide (NO) production at 100 &amp;#956;g/ml, while pan-fried LF showed little inhibitory effect on lipoloysaccharide (LPS) stimulated murine machrophage RAW264.7 cells. In contrast with its effect on...
Tipo: Journal article
Palavras-chave: Chlorogenic acid; Inflammation; Ligularia fischeri; Macrophage.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100063

Translational control of ceruloplasmin gene expression: Beyond the IRE
Provedor de dados: 48
Autores: MAZUMDER,BARSANJIT; SAMPATH,PRABHA; FOX,PAUL L.
Translational control is a common regulatory mechanism for the expression of iron-related proteins. For example, three enzymes involved in erythrocyte development are regulated by three different control mechanisms: globin synthesis is modulated by heme-regulated translational inhibitor; erythroid 5-aminolevulinate synthase translation is inhibited by binding of the iron regulatory protein to the iron response element in the 5'-untranslated region (UTR); and 15-lipoxygenase is regulated by specific proteins binding to the 3'-UTR. Ceruloplasmin (Cp) is a multi-functional, copper protein made primarily by the liver and by activated macrophages. Cp has important roles in iron homeostasis and in inflammation. Its role in iron metabolism was originally proposed...
Tipo: Journal article
Palavras-chave: Translational control; Ceruloplasmin; Iron homeostasis; GAIT element; Inflammation.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100007

Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
Provedor de dados: 48
Autores: Yin,Lei-Miao; Wei,Ying; Wang,Wen-Qian; Wang,Yu; Xu,Yu-Dong; Yang,Yong-Qing.
BACKGROUND: BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells. RESULTS: The WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the...
Tipo: Journal article
Palavras-chave: BrdU; WST-1; Cell proliferation; Cell viability; Airway smooth muscle cells.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100069

Histochemical detection of sugar residues in lizard teeth (Liolaemus gravenhorsti): a lectin-binding study
Provedor de dados: 48
Autores: FUENZALIDA,MARCELA; LEMUS,SUSI; ILLANES,JULIO; MONTIEL,ENRIQUE; ACUÑA,OLGA; LEMUS,DAVID.
The structural diversity of the many oligosaccharide chains of surface glycoconjugates renders them likely candidates for modulators of cell-interactions, cellular movements, differentiation, and cellular recognition. A selection of different lectins was used to investigate the appearance of cellular distribution and changes in sugar residues during tooth development in the polyphyodont lizard, Liolaemus gravenhorsti. Lectins from three groups were used: (1) N-acetylgalactosamine specificity: BS-1, PNA, RCA-120; (2) N-acetylglucosamine specificity: ECA; and (3) fucose specificity: UEA 1 and LTA.. Digital images were processed using Scion Image. Grayscale graphics in each image were obtained. The lectins used showed a strong, wide distribution of the...
Tipo: Journal article
Palavras-chave: Sugar residues; Teeth lizard HRP-lectins.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300008

High-level expression of modified gene encoding human adiponectin in transgenic rice
Provedor de dados: 48
Autores: Liu,Feng; Zhao,Yi-Ying; Zheng,Jin-Gui.
Adiponectin is a polypeptide specifically secreted from human adipocytes, and its deficiency is closely linked to increased obesity and type II diabetes. There is an urgent demand for large-scale production of human adiponectin for pharmaceutical applications. Here, we report that we have successfully obtained a high-level of expression of modified genes encoding human adiponectin in transgenic rice. The 735 bp cDNA of the native human sequence was adopted to rice codon usage, fused to the translation initiation sequence in the N terminus and to the KDEL signal sequence in the C terminus. An amplification promoting sequence acting as an enhancer of transcription was also introduced to enhance gene expression. The presence of the transgene and mRNA...
Tipo: Journal article
Palavras-chave: Human adiponectin; Transgenic rice; Modified gene; Anti-diabetic.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400009

Ascorbate protects (+)-catechin from oxidation both in a pure chemical system and human plasma
Provedor de dados: 48
Autores: LOTITO,SILVINA B; FRAGA,CESAR G.
We evaluated the interaction between ascorbic acid (AA) and (+)-catechin (CTCH) in potassium phosphate solution, pH 7.4 (PPS) and in human plasma. In both systems, the oxidation was started by adding 2,2’-azobis-(2-amidinopropane) clorhidrate (AAPH). The concentrations of AA and CTCH were determined by HPLC using electrochemical detection. In PPS, CTCH was oxidized by AAPH (50 muM), in either the absence or presence of different initial concentrations of AA (25-200 muM). In the presence of AA, CTCH depletion was delayed, an effect that was dependent upon the initial concentration of AA. When 100 muM AA was added after the oxidation had begun, CTCH depletion was arrested for 30 min. The kinetics of AA oxidation by AAPH was also characterized in PPS. AA (100...
Tipo: Journal article
Palavras-chave: Catechins; Antioxidants; Free radicals; Procyanidins.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200015

The effect of temperature and irradiance on the growth and carotenogenic capacity of seven strains of Dunaliella salina (Chlorophyta) cultivated under laboratory conditions
Provedor de dados: 48
Autores: GÓMEZ,PATRICIA I; GONZÁLEZ,MARIELA A.
The carotenogenic microalga Dunaliella salina is cultivated as a natural source of ß-carotene. The 9-cis isomer of ß-carotene is found only in natural sources having commercial advantages over the all-trans isomer due to its high liposolubility and antioxidant power. High irradiance appears to stimulate specifically all-trans ß-carotene accumulationin D. salina, whereas low temperature apparently elicits ß-carotene and 9-cis ß-carotene production. We studied the effect of temperature and irradiance on the growth and the carotenogenesis of three Chilean (CONC-001, CONC-006 and CONC-007) and four non-Chilean (from Mexico, China, Australia and Israel) strains of D. salina cultivated under two photon flux densities (40 and 110 &amp;#956;mol photons.m-2.s-1)...
Tipo: Journal article
Palavras-chave: Dunaliella salina strains growth &amp;#ß; -carotene ß-carotene temperature irradiance.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200005

Comparative study of inter-and intrahemispheric cortico-cortical connections in gerbil auditory cortex
Provedor de dados: 48
Autores: THOMAS,HARDY; LÓPEZ,VLADIMIR.
Topographic distributions and laminar pattern of cortico-cortical projections from the primary auditory field (AI), anterior auditory field (AAF), dorsoposterior field (DP), ventroposterior field (VP), dorsal field (D) and ventral field (V) were studied in relation to tonotopic maps in combined anatomical, electrophysiological and 2-deoxyfluoro-D-glucose (2DG) experiments. Distributions of axons were examined by means of retrogradely-transported fluorescent tracer Fast Blue (FB) injected in the primary (AI) and anterior (AAF) auditory field. Injections of fluorescent tracer were placed in electrophysiologically-identified locations of AI and AAF. Neurons in AAF, DP, VP and V project to AI in the ipsilateral hemisphere. This area also receives projections...
Tipo: Journal article
Palavras-chave: Auditory cortex; Cortical connections; Laminar patterns; Fluorescent tracer-retrograde labeling.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200006

Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
Provedor de dados: 48
Autores: DÍAZ-GRIFFERO,FELIPE; ESPINOZA CANCINO,CARMEN; MEDINA ARÉVALO,CONSUELO; ARCE-JOHNSON,PATRICIO.
Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Cg function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection, local necrotic lesions were developed in resistant transgenic plants, and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants, causing the same...
Tipo: Journal article
Palavras-chave: Movement protein; Crucifer-infecting tobamovirus; Viral complementation.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200009

p53-Independent checkpoint controls in a plant cell model
Provedor de dados: 48
Autores: PELAYO,HELVIA R; PINCHEIRA,JUANA; GIMÉNEZ-ABIÁN,JUAN F; CLARKE,DUNCAN J; DE LA TORRE,CONSUELO.
Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous override, cells became accumulated in G2-prophase, giving rise later on to a delayed mitotic wave. Cell growth was maintained during the checkpoint blocks, as the delayed mitoses were larger in size than the control ones. Under continuous hydroxyurea treatment, the delayed mitotic was formed by two subpopulations: normal mitoses corresponding to cells having properly recovered from the checkpoint block, and abnormal ones resulting from checkpoint adaptation. These latter cells displayed broken chromatids as they had...
Tipo: Journal article
Palavras-chave: Checkpoint adaptation; Hydroxyurea; S and G2 checkpoints; P53-independent checkpoints; Allium cepa L. meristems.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300009

Some considerations about the theory of intelligent design
Provedor de dados: 48
Autores: CARREÑO,JUAN E; HANSEN,FERNANDO; IRARRÁZABAL,MATÍAS; PHILIPPI,RODOLFO; CORREA,MATÍAS; BORJA,FRANCISCO; ADRIASOLA,CRISTÓBAL; SILVA,FRANCISCO; SERANI,ALEJANDRO.
The so-called theory of intelligent design (ID) has gained a growing reputation in the Anglo-Saxon culture, becoming a subject of public debate. The approaches that constitute the core of this proposal, however, have been poorly characterized and systematized. The three most significant authors of ID are certainly Michael Behe, William Dembski and Stephen Meyer. Beyond the differences that can be distinguished in the work of each of them, the central fact in their arguments is the complexity of living organisms, which according to these authors, escapes any kind of natural explanation. In effect, according to the authors of ID, the irreducible complexity that can be detected in the natural world would allow to infer design in a scientifically valid way,...
Tipo: Journal article
Palavras-chave: Epistemology; Evolution; Intelligent design; Science.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200011

Association of serum total bilirubin levels with diastolic dysfunction in heart failure with preserved ejection fraction
Provedor de dados: 48
Autores: Zheng,Huan; Li,Ye; Xie,Nanzi.
BACKGROUND: Left ventricular diastolic dysfunction is one of the main characteristics of heart failure patients with a preserved left ventricular ejection fraction. As bilirubin is regarded as an important endogenous antioxidant molecule, serum total bilirubin levels were compared between heart failure patients with a preserved left ventricular ejection fraction and normal controls in this study. We recruited 327 heart failure patients with a preserved left ventricular ejection fraction and 200 healthy controls. Patients were divided into 4 subgroups by their comprehensive echocardiographic manifestations, 1-mild, 2-moderate, 3-severe (reversible restrictive), 4-severe (fixed restrictive). Total bilirubin levels were compared using stepwise multiple...
Tipo: Journal article
Palavras-chave: Bilirubin; Heart failure; Diastolic dysfunction; Echocardiography.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100007

Association of the infusion of Heteropterys aphrodisiaca and endurance training brings spermatogenetic advantages
Provedor de dados: 48
Autores: Gomes,Marcos L M; Monteiro,Juliana C; Freitas,Karine M; Sbervelheri,Mariana M; Dolder,Heidi.
The species Heteropterys aphrodisiaca is commonly used as a stimulant by popular medicine in the Cerrado, a savanna-like biome, Brazil. Recent studies have proved its protective effects on testes of animals submitted to treatment using Cyclosporine A, as well as its stimulus effect in increasing testosterone secretion. Therefore, the present study was designed to analyze whether the association of the plant infusion and endurance exercise could potentiate the stimulating effect. The animals were separated into 4 groups: two control (sedentary and trained) receiving water and two treated (sedentary and trained) receiving the plant infusion daily (104mg/day). The proportion of the seminiferous tubule compartment and interstitium was analyzed. Within the...
Tipo: Journal article
Palavras-chave: Germ cells; Phytotherapy; Seminiferous epithelium; Spermatogenesis; Testosterone.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300004

Chagas disease: Present status of pathogenic mechanisms and chemotherapy
Provedor de dados: 48
Autores: Maya,Juan Diego; Orellana,Myriam; Ferreira,Jorge; Kemmerling,Ulrike; López-Muñoz,Rodrigo; Morello,Antonio.
There are approximately 7.8 million people in Latin America, including Chile, who suffer from Chagas disease and another 28 million who are at risk of contracting it. Chagas is caused by the flagellate protozoan Trypanosoma cruzi. It is a chronic disease, where 20%-30% of infected individuals develop severe cardiopathy, with heart failure and potentially fatal arrhythmias. Currently, Chagas disease treatment is more effective in the acute phase, but does not always produce complete parasite eradication during indeterminate and chronic phases. At present, only nifurtimox or benznidazole have been proven to be superior to new drugs being tested. Therefore, it is necessary to find alternative approaches to treatment of chronic Chagas. The current treatment...
Tipo: Journal article
Palavras-chave: Chagas disease; Chemotherapy; Nifurtimox; Trypanosoma cruzi; Prostaglandins.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300009

Antioxidant Properties and Total Phenolic Content of Eight Salvia Species from Turkey
Provedor de dados: 48
Autores: TOSUN,MURAT; ERCISLI,SEZAI; SENGUL,MEMNUNE; OZER,HAKAN; POLAT,TASKIN; OZTURK,ERDOGAN.
Methanolic extracts of eight Salvia species, namely S. aethiopis, S. candidissima, S. limbata, S. microstegia, S. nemorosa, S. pachystachys, S. verticillata, and S. virgata, sampled from Eastern Anatolia in Turkey, were screened for their possible antioxidant activities by two complementary test systems, namely DPPH free radical scavenging and b-carotene/linoleic acid. Total phenolic content of the extracts of Salvia species were performed Folin-Ciocalteu reagent and gallic acid used as standard. A wide variation has been observed among species in terms of antioxidant activity and total phenolic content. In both DPPH and b-carotene system, the most active plant was Salvia verticillata with a value of IC50=18.3 &amp;#956;g/ml and 75.8%, respectively. This...
Tipo: Journal article
Palavras-chave: Sage; Salvia; Total phenolic content.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200005

Regional density of glial cells in the rat corpus callosum
Provedor de dados: 48
Autores: Reyes-Haro,Daniel; Mora-Loyola,Ernesto; Soria-Ortiz,Berenice; García-Colunga,Jesús.
Axons and glial cells are the main components of white matter. The corpus callosum (CC) is the largest white matter tract in mammals; in rodents, 99% of the cells correspond to glia after postnatal day 5 (P5). The area of the CC varies through life and regional differences related to the number of axons have been previously described. Whether glial cell density varies accordingly is unknown; thus the aim of this study was to estimate glial cell density for the genu, body and splenium -the three main regions of CC-, of P6 and P30 rats. Here we report that the density of CC glial cells reduced by ~10% from P6 to P30. Even so, the density of astrocytes showed a slight increase (+6%), probably due to differentiation of glioblasts. Interestingly, glial cell...
Tipo: Journal article
Palavras-chave: Astrocytes; Corpus callosum; Genu; Glial cells; Splenium.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100004

La Educación en Ciencias Biológicas demanda urgentes cambios
Provedor de dados: 48
Autores: Krauskopf,Manuel.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200001

Human epididymal proteins and sperm function during fertilization: un update
Provedor de dados: 48
Autores: LASSERRE,ANDREA; BARROZO,ROMINA; TEZÓN,JORGE G.; MIRANDA,PATRICIA V.; VAZQUEZ-LEVIN,MÓNICA H.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300004

In Osteoporosis, differentiation of mesenchymal stem cells (MSCs) improves bone marrow adipogenesis
Provedor de dados: 48
Autores: Pino,Ana María; Rosen,Clifford J; Rodríguez,J. Pablo.
The formation, maintenance, and repair of bone tissue involve close interlinks between two stem cell types housed in the bone marrow: the hematologic stem cell originating osteoclasts and mesenchymal stromal cells (MSCs) generating osteoblasts. In this review, we consider malfunctioning of MSCs as essential for osteoporosis. In osteoporosis, increased bone fragility and susceptibility to fractures result from increased osteoclastogenesis and insufficient osteoblastogenesis. MSCs are the common precursors for both osteoblasts and adipocytes, among other cell types. MSCs' commitment towards either the osteoblast or adipocyte lineages depends on suitable regulatory factors activating lineage-specific transcriptional regulators. In osteoporosis, the reciprocal...
Tipo: Journal article
Palavras-chave: MSCs; Osteoporosis; Adipogenesis; Bone marrow microenvironment.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300009

The effects of 4-nonylphenol contamination on livers of Tilapia fish (Oreochromus spilurs) in Jeddah
Provedor de dados: 48
Autores: Abdulla Bin-Dohaish,El-jawaher.
Alkylphenol polyethoxylates is a group of estrogenic compounds. Natural or synthetic types of these compounds react with the endocrine system by binding hormone receptors, resulting in interference with their action, which is why they are called endocrine disrupting chemicals. Among their hydrolytic products are nonylphenols (NP), which are considered pollutants of aquatic environments. The objective of this study was to evaluate the pathological alterations on liver tissue of fish exposed to these compounds for long durations, starting from beginning of life and during the period of sexual maturity. Tilapia fish were obtained from Abhur fish farms, reared in the laboratory in special basins, and divided into two groups. The first maternal group was...
Tipo: Journal article
Palavras-chave: Nonylphenol; Tilapia fish; Liver; Histology.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100002

Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
Provedor de dados: 48
Autores: Mahesh,Ayyavu; Thangadurai,Devarajan; Melchias,Gabriel.
An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
Tipo: Journal article
Palavras-chave: Asteraceae; Launaea sarmentosa; Leaf explant; Organogenesis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200004

20 AÑOS DE FONDECYT
Provedor de dados: 48
Autores: PHILIPPI,BRUNO.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100003

Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
Provedor de dados: 48
Autores: Matei,Clara; Tampa,Mircea; Caruntu,Constantin; Ion,Rodica-Mariana; Georgescu,Simona-Roxana; Dumitrascu,Georgiana Roxana; Constantin,Carolina; Neagu,Monica.
BACKGROUND: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential. RESULTS: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral...
Tipo: Journal article
Palavras-chave: Aluminum di-sulphonated phthalocyanine; DOK cells; Photodynamic therapy; Protein microarray.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100033

PANELES II
Provedor de dados: 48
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000300007

A neutral cue facilitates detection of a visual target by modulating attention
Provedor de dados: 48
Autores: HAMAME,CARLOS M; DELANO,PAÚL H; ROBLES,LUIS.
Twelve rats were trained to perform a two-choice visual detection task in which a right or left light was presented and the animáis were required to press the lever located under the illuminated light for a food reward. In seventy percent of the triáis the target light was preceded by presentation of a neutral cue (a central light). Relevance of the neutral cue for detection of the target was analyzed by comparing behavioral Índices of attention in its presence and absence. Accuracy was significantly higher in presence than in absence of the neutral cue, while mean response latencies were lower in presence than in absence of the neutral cue. These results indicate that the animáis allocated attentional resources on the target detection during a high...
Tipo: Journal article
Palavras-chave: Alerting effect; Attention; Detection task; Expectancy; Neutral cue; Rat.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400012

Fast kinetics of calcium dissociation from calsequestrin
Provedor de dados: 48
Autores: BELTRÁN,MARIANELA; BARRIENTOS,GENARO; HIDALGO,CECILIA.
We measured the kinetics of calcium dissociation from calsequestrin in solution or forming part of isolated junctional sarcoplasmic reticulum membranes by mixing calsequestrin equilibrated with calcium with calcium-free solutions in a stopped-flow system. In parallel, we measured the kinetics of the intrinsic fluorescence changes that take place following calcium dissociation from calsequestrin. We found that at 25ºC calcium dissociation was 10-fold faster for calsequestrin attached to junctional membranes (k = 109 s-1) than in solution. These results imply that calcium dissociation from calsequestrin in vivo is not rate limiting during excitation-contraction coupling. In addition, we found that the intrinsic fluorescence decrease for calsequestrin in...
Tipo: Journal article
Palavras-chave: Calcium-binding proteins; Ryanodine receptors; Sarcoplasmic reticulum; Calcium release kinetics; Excitation-contraction coupling; Skeletal and cardiac muscle.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300011

Effects of neuromuscular blocking agents on central respiratory chemosensitivity in newborn rats
Provedor de dados: 48
Autores: SAKURABA,SHIGEKI; KUWANA,SHUN-ICHI; ERIKSSON,LARS I; OKADA,YASUMASA; OCHIAI,RYOICHI; KASHIWAGI,MASANORI; HATORI,EIKI; LINDAHL,STEN G. E.; TAKEDA,JUNZO.
Neuromuscular blocking agents suppress central respiratory activity through their inhibitory effects on preinspiratory neurons and the synaptic drive from preinspiratory neurons to inspiratory neurons. Central CO2-chemosensitive areas, which partly consist of CO2-excited neurons, in the rostral ventrolateral medulla are thought to provide tonic drive to the central respiratory network and involve cholinergic mechanisms, which led us to hypothesize that neuromuscular blocking agents can inhibit CO2-excited neurons and attenuate respiratory CO2 responsiveness. To test this hypothesis, we used isolated brainstem-spinal cord preparations from newborn rats. The increase of C4 burst frequency induced by a hypercapnic superfusate, i.e. respiratory CO2...
Tipo: Journal article
Palavras-chave: Brainstem; Chemosensitivity; CO2 responsiveness; Muscle relaxant; Nicotinic acetylcholine.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200012

Large conductance Ca2+-activated K+ (BK) channel: Activation by Ca2+ and voltage
Provedor de dados: 48
Autores: LATORRE,RAMÓN; BRAUCHI,SEBASTIAN.
Large conductance Ca2+-activated K+ (BK) channels belong to the S4 superfamily of K+ channels that include voltage-dependent K+ (Kv) channels characterized by having six (S1-S6) transmembrane domains and a positively charged S4 domain. As Kv channels, BK channels contain a S4 domain, but they have an extra (S0) transmembrane domain that leads to an external NH2-terminus. The BK channel is activated by internal Ca2+, and using chimeric channels and mutagenesis, three distinct Ca2+-dependent regulatory mechanisms with different divalent cation selectivity have been identified in its large COOH-terminus. Two of these putative Ca2+-binding domains activate the BK channel when cytoplasmic Ca2+ reaches micromolar concentrations, and a low Ca2+ affinity mechanism...
Tipo: Journal article
Palavras-chave: BK channel; Ca2+-binding sites; Voltage dependence; Allosteric models.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300003

Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae
Provedor de dados: 48
Autores: Vásquez-Soto,Beatriz; Manríquez,Nicolás; Cruz-Amaya,Mirna; Zouhar,Jan; Raikhel,Natasha V; Norambuena,Lorena.
BACKGROUND: A highly regulated trafficking of cargo vesicles in eukaryotes performs protein delivery to a variety of cellular compartments of endomembrane system. The two main routes, the secretory and the endocytic pathways have pivotal functions in uni- and multi-cellular organisms. Protein delivery and targeting includes cargo recognition, vesicle formation and fusion. Developing new tools to modulate protein trafficking allows better understanding the endomembrane system mechanisms and their regulation. The compound Sortin2 has been described as a protein trafficking modulator affecting targeting of the vacuolar protein carboxypeptidase Y (CPY), triggering its secretion in Saccharomyces cerevisiae. RESULTS: A reverse chemical-genetics approach was used...
Tipo: Journal article
Palavras-chave: Endocytosis; Sortin2; Trafficking modulator; Saccharomyces cerevisiae.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100039

Plant genomics: an overview
Provedor de dados: 48
Autores: CAMPOS-DE QUIROZ,HUGO.
Recent technological advancements have substantially expanded our ability to analyze and understand plant genomes and to reduce the gap existing between genotype and phenotype. The fast evolving field of genomics allows scientists to analyze thousand of genes in parallel, to understand the genetic architecture of plant genomes and also to isolate the genes responsible for mutations. Furthermore, whole genomes can now be sequenced. This review addresses these issues and also discusses ways to extract biological meaning from DNA data. Although genomic issuesare addressed from a plant perspective, this review provides insights into the genomic analyses of other organisms
Tipo: Journal article
Palavras-chave: Genomics; Plant breeding; Gene discovery.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300013

The S6 kinase Signaling Pathway In The Control of Development and Growth
Provedor de dados: 48
Autores: THOMAS,GEORGE.
The discussion will focus on the role of the ribosomal protein S6 kinase (S6K) signaling pathway in the regulation of cell growth and proliferation. Although 40S ribosomal protein S6 phosphorylation was first described 25 years ago (&lt;A HREF="#20"&gt;Gressner and Wool, 1974&lt;/A&gt;), it only recently has been implicated in the translational up-regulation of mRNAs coding for the components of protein synthetic apparatus (&lt;A HREF="#15"&gt;Fumagalli and Thomas, 2000&lt;/A&gt;). These mRNAs contain an oligopyrimidine tract at their 5' transcriptional start site, termed a 5'TOP, which has been shown to be essential for their regulation at the translational level (&lt;A HREF="#32"&gt;Meyuhas et al., 1996&lt;/A&gt;). In parallel, a great deal of...
Tipo: Journal article
Palavras-chave: Drosophila; SScells; Diabetes.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200022

Exosomes: mediators of communication in eukaryotes
Provedor de dados: 48
Autores: Lopez-Verrilli,María A; Court,Felipe A.
In addition to the established mechanisms of intercellular signaling, a new way of communication has gained much attention in the last decade: communication mediated by exosomes. Exosomes are nanovesicles (with a diameter of 40-120 nm) secreted into the extracellular space by the multivesicular endosome after its outer membrane fuses with the plasma membrane. Once released, exosomes modulate the response of the recipient cells that recognize them. This indicates that exosomes operate in a specific manner and participate in the regulation of the target cell. Remarkably, exosomes occur from unicellular organisms to mammals, suggesting an evolutionarily conserved mechanism of communication. In this review we describe the cascade of exosome formation,...
Tipo: Journal article
Palavras-chave: Exosome; Nanovesicle; Intercellular communication.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100001

Effect of the zinc chelator N,N,N',N'-tetrakis (2-pyridylmethyl)ethylenediamine (TPEN) on hippocampal mossy fiber calcium signals and on synaptic transmission
Provedor de dados: 48
Autores: MATIAS,CARLOS M; MATOS,NUNO C; ARIF,MONA; DIONISIO,JOSE C; QUINTA-FERREIRA,M EMILIA.
An important pool of chelatable zinc is present in the synaptic vesicles of mossy fiber terminals from hippocampal CA3 area, being zinc released following single or repetitive electrical stimulation. Previous studies have suggested different synaptic roles for released mossy fiber zinc, including the inhibition of presynaptic calcium and of postsynaptic N-methyl-D-aspartate (NMDA) and gamma amino-butiric acid (GABA A) receptors. The effect of endogenously released zinc on mossy fiber long-term potentiation (LTP) induction also is not yet established. We have investigated the effect of the permeant zinc chelator N,N,N',N'-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) on mossy fiber calcium and on synaptic transmission, before and during the application...
Tipo: Journal article
Palavras-chave: Mossy fiber synapses; Long-term potentiation (LTP); Area CA3; Presynaptic calcium; Fura-2; Area CA1.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300013

A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting
Provedor de dados: 48
Autores: Acharya,Arpan; Vaniawala,Salil; Shah,Parth; Parekh,Harsh; Misra,Rabindra Nath; Wani,Minal; Mukhopadhyaya,Pratap N.
BACKGROUND: Human Immunodeficiency Virus Type 1 (HIV-1) viral load testing at regular intervals is an integral component of disease management in Acquired Immunodeficiency Syndrome (AIDS) patients. The need in countries like India is therefore an assay that is not only economical but efficient and highly specific for HIV-1 sub type C virus. This study reports a SYBR Green-based HIV-1 real time PCR assay for viral load testing and is designed for enhanced specificity towards HIV-1 sub type C viruses prevalent in India. RESULTS: Linear regression of the observed and reference concentration of standards used in this study generated a correlation coefficient of 0.998 (p&amp;#8201;&lt;&amp;#8201;0.001). Lower limit of detection of the test protocol was 50...
Tipo: Journal article
Palavras-chave: HIV-1; Gag; SYBR Green; RT PCR.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100022

INCORPORACIONES
Provedor de dados: 48
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000300004

Transcriptome analysis and crop improvement: (A review)
Provedor de dados: 48
Autores: DUNWELL,JIM M; MOYA-LEÓN,MARIA A; HERRERA,RAUL.
The identification and characterization of differential gene expression from tissues subjected to stress has gained much attention in plant research. The recognition of elements involved in the response to a particular stress enhances the possibility of promoting crop improvement through direct genetic modification. However, the performance of some of the `first generation' of transgenic plants with the incorporation of a single gene has not always been as expected. These results have stimulated the development of new transgenic constructions introducing more than one gene and capable of modifying complex pathways. Several techniques are available to conduct the analysis of gene regulation, with such information providing the basis for novel constructs...
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300003

Using genomics to improve fruit quality
Provedor de dados: 48
Autores: Meneses,Claudio; Orellana,Ariel.
New fruit varieties are needed to satisfy consumers, and the industry is facing new challenges in order to respond to these demands. The emergence of genomic tools is releasing information on polymorphisms that can be utilized to expedite breeding processes in species that are difficult to breed, given the long periods of time required to get new varieties. The present review describes the current stages of the ongoing efforts that are being taken to apply these technologies to obtain varieties with improved fruit quality in species of the family Rosaceae.
Tipo: Journal article
Palavras-chave: Polymorphisms; Marker Assited Selection; Genomic Tools.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400006

Antioxidant and anticholinesterase investigations of Rumex hastatus D. Don: potential effectiveness in oxidative stress and neurological disorders
Provedor de dados: 48
Autores: Ahmad,Sajjad; Ullah,Farhat; Ayaz,Muhammad; Sadiq,Abdul; Imran,Muhammad.
BACKGROUND: Rumex species are traditionally used for the treatment of neurological disorders including headache, migraine, depression, paralysis etc. Several species have been scientifically validated for antioxidant and anticholinestrase potentials. This study aims to investigate Rumex hastatus D. Don crude methanolic extract, subsequent fractions, saponins and flavonoids for acetylcholinestrase, butyrylcholinestrase inhibition and diverse antioxidant activities to validate its folkloric uses in neurological disorders. Rumexhastatus crude methanolic extract (Rh. Cr), subsequent fractions; n-hexane (Rh. Hex), chloroform (Rh. Chf), ethyl acetate (Rh. EtAc), aqueous fraction (Rh. Aq), crude saponins (Rh. Sp) and flavonoids (Rh. Fl) were investigated against...
Tipo: Journal article
Palavras-chave: Ethnomedicinal; Acetylcholinesterase; Butyrycholinesterase; DPPH; ABTS.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100020

Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
Provedor de dados: 48
Autores: Ilavenil,Soundharrajan; Kim,Da Hye; Vijayakumar,Mayakrishnan; Srigopalram,Srisesharam; Roh,Sang Gun; Arasu,Mariadhas Valan; Lee,Jong Suk; Choi,Ki Choon.
BACKGROUND: From ancient times, marine algae have emerged as alternative medicine and foods, contains the rich source of natural products like proteins, vitamins, and secondary metabolites, especially Chlorella vulgaris (C. vulgaris) contains numerous anti-inflammatory, antioxidants and wound healing substances. Type 2 diabetes mellitus is closely associated with adipogenesis and their factors. Hence, we aimed to investigate the chemical constituents and adipo-genic modulatory properties of C. vulgaris in 3T3-L1 pre-adipocytes. RESULTS: We analysed chemical constituents in ethanolic extract of C. vulgaris (EECV) by LC-MS. Results revealed that the EECV contains few triterpenoids and saponin compounds. Further, the effect of EECV on lipid accumulation along...
Tipo: Journal article
Palavras-chave: Chlorella vulgaris Chemical constituents Adipocyte differentiation PPAR-&amp;#947; 2 AMPK-a.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100038

Non-homogeneous spatial configuration of vibrissae cortical representation in layer IV of the barrel somatosensory cortex
Provedor de dados: 48
Autores: GUIC,ELIANA; CARRASCO,XIMENA; RODRÍGUEZ,EUGENIO; ROBLES,IGNACIO; MERZENICH,MICHAEL M.
In the present experiments we studied exclusive and overlapping cortical representational áreas of the vibrissae in layer IV cells, across the entire barrel subfield of the rat somatosensory cortex, looking for evidences that would challenge the present assumptions of homogeneity and symmetry among cortical columns in this sensorial system. Our main findings were that in layer IV of the rat barrel cortex: A) Size of vibrissae cortical representational áreas (X=0.4174mm²; SD=0.025) was not homo geneous, vibrissae in dorsal rows (A-B) had significantly smaller áreas than those in ventral rows (D-E), a pattern that repeated itself in ares 1-4. B) This difference arises from vibrissal representational overlap, and not from variations in exclusive zones, which...
Tipo: Journal article
Palavras-chave: Cortical representation; Barrel cortex; Evoked activity; Rat -somatosensory cortex; Spatial configuration.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400011

Study of Cytochrome P450 2E1 and its allele Variants in Liver Injury of Nondiabetic, Nonalcoholic Steatohepatitis Obese Women
Provedor de dados: 48
Autores: VARELA,NELSON M; QUIÑONES,LUIS A; ORELLANA,MYRIAM; PONIACHIK,JAIME; CSENDES,ATTILA; SMOK,GLADYS; RODRIGO,RAMÓN; CÁCERES,DANTE D; VIDELA,LUIS A.
CYP2E1 enzyme is related to nonalcoholic steatohepatitis (NASH) due to its ability for reactive oxygen species production, which can be influenced by polymorphisms in the gene. The aim of this study was to investigate hepatic levels, activity, and polymorphisms of the CYP2E1 gene to correlate it with clinical and histological features in 48 female obese NASH patients. Subjects were divided into three groups: (i) normal; (ii) steatosis; and (iii) steatohepatitis. CYP2E1 protein level was assayed in microsomes from liver biopsies, and in vivo chlorzoxazone hydroxylation was determined by HPLC. Genomic DNA was isolated for genotype analysis through PCR. The results showed that liver CYP2E1 content was significantly higher in the steatohepatitis (45%; p=0.024)...
Tipo: Journal article
Palavras-chave: Steatohepatitis; NASH; CYP2E1; Obesity; Genotype; Chlorzoxazone.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100010

Immunoresponse of Coho salmon immunized with a gene expression library from Piscirickettsia salmonis
Provedor de dados: 48
Autores: MIQUEL,ALVARO; MÜLLER,ILSE; FERRER,PABLO; VALENZUELA,PABLO D. T.; BURZIO,LUIS O.
We have used the expression library immunization technology to study the protection of Coho salmon Oncorhynchus kisutch to the infection with Piscirickettsia salmonis. Purified DNA from this bacterium was sonicated and the fragments were cloned in the expression vector pCMV-Bios. Two libraries were obtained containing 22,000 and 28,000 colonies and corresponding to approximately 8 and 10 times the genome of the pathogen, respectively. On average, the size of the inserts ranged between 300 and 1,000 bp. The plasmid DNA isolated from one of these libraries was purified and 20 µg were injected intramuscularly into 60 fish followed by a second dose of 10 µg applied 40 days later. As control, fish were injected with the same amount of DNA of the vector...
Tipo: Journal article
Palavras-chave: Piscirickettsia salmonis; Genetic vaccine; Expression library immunization.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300004

Dimensional analysis revisited
Provedor de dados: 48
Autores: GÜNTHER,BRUNO; MORGADO,ENRIQUE.
The applicability of dimensional analysis (DA) is discussed in relation to the metabolic scaling laws. The evolution of different theories of biological similarity has shown that the calculated reduced exponents (b) of Huxley's allometric equation are closely correlated with the numerical values obtained from the statistical analysis of empirical data. Body mass and body weight are not equivalent as biological reference systems, since in accordance to Newton's second law, the former has a dimension of a mass, while the latter should be dimensionally considered as a force (W = MLT-2). This distinction affects the coefficients of the mass exponent (a). This difference is of paramount importance in microgravity conditions (spaceflight) and of buoyancy during...
Tipo: Journal article
Palavras-chave: Dimensional analysis; Allometric scaling; Theories of biological similarity; Body mass; Body weight.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300011

Morphometric and phytochemical characterization of chaura fruits (Gaultheria pumila): a native Chilean berry with commercial potential
Provedor de dados: 48
Autores: Villagra,Evelyn; Campos-Hernandez,Carola; Cáceres,Pablo; Cabrera,Gustavo; Bernardo,Yamilé; Arencibia,Ariel; Carrasco,Basilio; Caligari,Peter DS; Pico,José; García-Gonzales,Rolando.
BACKGROUND: For the first time, a morphometric characterization of chaura (Gaultheria pumila) fruits has been conducted between natural populations growing in the Villarrica National Park, Araucania Region, Chile. Chaura is a native Ericaceae from Chile that produces aromatic and tasty fruits which could be of agricultural interest. RESULTS: To influence the decision for a further domestication of G. pumila, both the fruit sizes (indicator of productivity) and the nutritional properties of the fruits have been determined from different subpopulations. Samples were a total of 74 plants and 15 fruits per plant which were randomly harvested following its natural distribution around the Villarrica volcano. Altogether, fresh weight, shape, color, diameter in...
Tipo: Journal article
Palavras-chave: Gaultheria pumila; Fruit morphometry; Fruit diversity; Pectin; Anthocyanins.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100026

Effects of heat stress on some blood parameters and egg production of Shika Brown layer chickens transported by road
Provedor de dados: 48
Autores: Ajakaiye,Joachim J; Ayo,Joseph O; Ojo,Sunday A.
The effects of vitamins C and E on layer chickens transported by road for 6 h during the hot dry season were investigated. Two experimental groups consisting of thirty Shika Brown layers were separately administered vitamins C and E orally just before transportation, while another 30 layers, which were only given sterile water, served as control. Blood samples analyzed before and after transportation in the control layers showed a decrease (p&lt;0.05) in total white blood cell, (p&lt;0.01) lymphocyte and monocyte values, and a signifcant (p&lt;0.05) and (p&lt;0.001) increase in the values of eosinophils and heterophils post-transportation, respectively. In the experimental groups, post-transportation values of total white blood cells, eosinophils and...
Tipo: Journal article
Palavras-chave: Egg production; Heat and transport stress; Layer chickens; Leukocyte profle; Vitamins C and E.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200006

Misclassification probability as obese or lean in hypercaloric and normocaloric diet
Provedor de dados: 48
Autores: NASCIMENTO,ANDRÉ F; SUGIZAKI,MÁRIO M; LEOPOLDO,ANDRÉ S; LIMA-LEOPOLDO,ANA P; NOGUEIRA,CÉLIA R; NOVELLI,ETHEL L. B; PADOVANI,CARLOS R; CICOGNA,ANTONIO C.
The aim of the present study was to determine the classification error probabilities, as lean or obese, in hypercaloric diet-induced obesity, which depends on the variable used to characterize animal obesity. In addition, the misclassification probabilities in animáis submitted to normocaloric diet were also evaluated. Male Wistar rats were randomly distributed into two groups: normal diet (ND; n=31; 3,5 Kcal/g) and hypercaloric diet (HD; n=31; 4,6 Kcal/g). The ND group received commercial Labina rat feed and HD animáis a cycle of five hypercaloric diets for a 14-week period. The variables analysed were body weight, body composition, body weight to length ratio, Lee Índex, body mass Índex and misclassification probability. A 5% significance level was used....
Tipo: Journal article
Palavras-chave: Diet; Diet-induced obesity; Hypercaloric diet; Misclassification; Rats.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300002

Stem cells in embryonic skin development
Provedor de dados: 48
Autores: Forni,Maria F; Trombetta-Lima,Marina; Sogayar,Mari C.
The skin is a complex stratified organ which acts not only as a permeability barrier and defense against external agents, but also has essential thermoregulatory, sensory and metabolic functions. Due to its high versatility and activity, the skin undergoes continuous self-renewal to repair damaged tissue and replace old cells. Consequently, the skin is a reservoir for adult stem cells of different embryonic origins. Skin stem cell populations reside in the adult hair follicle, sebaceous gland, dermis and epidermis. However, the origin of most of the stem cell populations found in the adult epidermis is still unknown. Far more unknown is the embryonic origin of other stem cells that populate the other layers of this tissue. In this review we attempt to...
Tipo: Journal article
Palavras-chave: Skin development; Embryonic skin; Epidermis; Dermis; Sebaceous Gland; Hair follicle.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300003

Melatonin Protects the Heart, Lungs and Kidneys from Oxidative Stress under Intermittent Hypobaric Hypoxia in Rats
Provedor de dados: 48
Autores: Farías,Jorge G; Zepeda,Andrea B; Calaf,Gloria M.
Melatonin (N-acetyl-5-methoxytryptamine) is the main secretory product of the pineal gland in all mammals including humans, but it is also produced in other organs. It has been previously demonstrated to be a powerful organ-protective substance under oxidative stress conditions. The aim of this study was to evaluate the protective effect of melatonin in several organs such as heart, lung, kidney, and of the reproductive system, such as testis and epididymis in animals exposed to intermittent hypobaric hypoxia and therefore exposed to oxidative stress and analyzed by lipid peroxidation. Ten-week-old male Wistar rats were divided into 6 groups for 96 hours during 32 days under: 1) Normobaric conditions, 2) plus physiologic solution, 3) plus melatonin, 4)...
Tipo: Journal article
Palavras-chave: Intermittent hypobaric hypoxia; Lipid peroxidation; Melatonin; Oxidative stress.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100011

Dysmyelination, demyelination and reactive astrogliosis in the optic nerve of the taiep rat
Provedor de dados: 48
Autores: KRSULOVIC,JUAN; COUVE,EDUARDO; RONCAGLIOLO,MANUEL.
Taiep is an autosomal recessive mutant rat that shows a highly hypomyelinated central nervous system (CNS). Oligodendrocytes accumulate microtubules (MTs) in association with endoplasmic reticulum (ER) membranes forming MT-ER complexes. The microtubular defect in oligodendrocytes, the abnormal formation of CNS myelin and the astrocytic reaction were characterized by immunocytochemical and ultrastructural methods during the first year of life. Optic nerves of both control and taiep rats were processed by the immunoperoxidase method using antibodies against tubulin, myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP). Taiep oligodendrocytes are strongly immunoreactive against tubulin, indicative of a significant accumulation of...
Tipo: Journal article
Palavras-chave: Myelin mutant; Taiep rat; Optic nerve; Dysmyelination; Demyelination; Astrogliosis.
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400005

FONDECYT Decreases Research Projects in Biological Sciences
Provedor de dados: 48
Autores: KRAUSKOPF,MANUEL.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100001

Phospholipid synthesis, diacylglycerol compartmentation, and apoptosis
Provedor de dados: 48
Autores: WRIGHT,MARCIA M.; MCMASTER,CHRISTOPHER R.
Apoptosis is a means by which organisms dispose of unwanted cells without inducing an inflammatory response. Alterations in apoptosis is a common process by which cells become cancerous. Paradoxically, many cancer chemotherapeutics preferentially kill cancer cells by inducing apoptosis. Diacylglycerol is a lipid second messenger that regulates cell growth and apoptosis and is produced during signal transduction by hydrolysis of membrane phospholipids. Protein kinase Cs are a family of diacylglycerol responsive enzymes that are recruited to cellular membranes as a consequence of diacylglycerol production where they phosphorylate specific target proteins responsible for regulating cell growth. In this review, we will first summarize our current understanding...
Tipo: Journal article
Palavras-chave: Apoptosis; Diacylglycerol; Farnesol; Phorbol ester; Protein kinase C.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200014

Induction of secondary metabolite production by UV-C radiation in Vitis vinifera L. Öküzgözü callus cultures
Provedor de dados: 48
Autores: Cetin,Emine Sema.
BACKGROUND: The aim of the present work was to examine the role of UV-C irradiation on the production of secondary metabolites (total phenolic, total flavanols, total flavonols, catechin, ferulic acid and trans-resveratrol in phenolic compounds and &amp;#945;-, &amp;#946;-, &amp;#947;- &amp;#948;-tocopherols) in callus cultures. Studies on the effects of UV-C treatment on callus culture are seldom and generally focused on UV-B. However UV-C radiation play an important role in accumule secondary metabolites. RESULTS: In this study, callus cultures from Öküzgözü grape cultivar were initiated from leaf petiole explants. Calli formed after 6 weeks on the medium supplemented with 0.5 mg L-1 benzylaminopurine (BA), 0.5 mg L-1 indole acetic acid (IAA) on B5...
Tipo: Journal article
Palavras-chave: Callus; Secondary metabolite; UV-C treatment.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100037

Plasticity in primary somatosensory cortex resulting from environmentally enriched stimulation and sensory discrimination training
Provedor de dados: 48
Autores: GUIC,ELIANA; CARRASCO,XIMENA; RODRÍGUEZ,EUGENIO; ROBLES,IGNACIO; MERZENICH,MICHAEL M.
We studied primary-somatosensory cortical plasticity due to selective stimulation of the sensory periphery by two procedures of active exploration in adult rats. Subjects, left with only three adjacent whiskers, were trained in a roughness discrimination task or maintained in a tactile enriched environment. Either training or enrichment produced 3-fold increases in the barrel cortex áreas of behaviorally-engaged whisker representations, in their zones of overlap. While the overall áreas of representation expanded dramatically, the domains of exclusive principal whisker responses were virtually identical in enriched vs normal rats and were significantly smaller than either group in roughness discrimination-trained rats. When animáis were trained or exposed...
Tipo: Journal article
Palavras-chave: Cortical plasticity; Roughness discrimination; Environmental enrichment; Barrel cortex; Active exploration.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400008

Hypercholesterolemia and tissue-specific differential mRNA expression of type-1 5'-iodothyronine deiodinase under different selenium status in rats
Provedor de dados: 48
Autores: DHINGRA,SANJIV; BANSAL,MOHINDER P.
Type-1 5'-iodothyronine deiodinase (5'-DI) is responsible for conversion of T4 to T3. Selenium (Se) is an integral part of this enzyme. Keeping in view the strong association between atherosclerosis and hypothyroidism, the present study examined the behavior of 5'-DI in liver, aorta and thyroid during hypercholesterolemia following different Se status, i.e., Se deficiency (0.02ppm), adequate (0.2ppm) and excess dose (1ppm) in SD male rats. Animals were fed a control or high-cholesterol diet (2%) for 1 and 2 months. 5'-DI activity and mRNA expression was measured by RIA and RT-PCR respectively. In liver and aorta, 5'-DI expression significantly decreased with the Se-deficient and the high-cholesterol diet. The trend was opposite in thyroid, i.e., mRNA...
Tipo: Journal article
Palavras-chave: Selenium; Hypercholesterolemia; 5'-DI; Cholesterol; RT-PCR.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200013

Editorial
Provedor de dados: 48
Autores: Arce,J. Patricio; Santos,Manuel J.
Tipo: Journal article
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400001

Effects of betamethasone, sulindac and quinacrine drugs on the inflammatory neoangiogenesis response induced by polyurethane sponge implanted in mouse
Provedor de dados: 48
Autores: ILLANES,JULIO; DABANCENS,ALFREDO; ACUÑA,OLGA; FUENZALIDA,MARCELA; GUERRERO,ANIBAL; LOPEZ,CLAUDIA; LEMUS,DAVID.
In this study, we showed the effect of the betamethasone, sulindac and quinacrine alone or combined, on the inflammatory angiogenesis promoted by polyurethane sponge on mice. The main finding reported here is that the formation of new blood vessels was strongly inhibited by low concentration of betamethasone, sulindac or quinacrine, whether alone or in combination. It is known that steroidal anti-inflammatory drugs inhibit the enzymes required for the production of prostaglandins through a nuclear glucocorticoid receptor (GR) mediated mechanism. This mechanism may occur in endothelial cells as well. Considering that activity of cyclo-oxigenases 1 and 2 is inhibited by sulindac, and that these enzymes are located in the stromal tissue, we propose that the...
Tipo: Journal article
Palavras-chave: Angiogenesis; Antiangiogenesis; Betamethasone; Sulindac; Quinacrine.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300008

Regulation of transepithelial transport of iron by hepcidin
Provedor de dados: 48
Autores: MENA,NATALIA P; ESPARZA,ANDRÉS L; NÚÑEZ,MARCO T.
Hepcidin (Hepc) is a 25 amino acid cationic peptide with broad antibacterial and antifungal actions. A likely role for Hepc in iron metabolism was suggested by the observation that mice having disruption of the gene encoding the transcription factor USF2 failed to produce Hepc mRNA and developed spontaneous visceral iron overload. Lately, Hepc has been considered the "stores regulator," a putative factor that signals the iron content of the body to intestinal cells. In this work, we characterized the effect of Hepc produced by hepatoma cells on iron absorption by intestinal cells. To that end, human Hepc cDNA was cloned and overexpressed in HepG2 cells and conditioned media from Hepc-overexpressing cells was used to study the effects of Hepc on intestinal...
Tipo: Journal article
Palavras-chave: Hepcidin; Iron absorption; DMT1; Caco-2 cells.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100022

IMMEDIACY INDEX EN LA CATEGORÍA "BIOLOGY": BIOLOGICAL RESEARCH ALCANZA EL QUINTO LUGAR ENTRE SUS CONGÉNERES DE ACUERDO AL JOURNAL OF CITATION REPORTS (2000)
Provedor de dados: 48
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200006

Discrimination of bile acids by the rainbow trout olfactory system: Evidence as potential pheromone
Provedor de dados: 48
Autores: GIAQUINTO,PERCILIA C; HARA,TOSHIAKI J.
Electro-olfactogram recording was used to determine whether the olfactory epithelium of adult rainbow trout is specifically sensitive to bile acids, some of which have been hypothesized to function as pheromones. Of 38 bile acids that had been pre-screened for olfactory activity, 6 were selected. The rainbow trout-specific bile acids, taurocholic acid (TCA), and taurolithocholic acid 3-sulfate (TLS) were the most potent compounds tested. TLS had a distinctive dose-response curve. Cross-adaptation experiments demonstrated that sensitivity to bile acids is attributable to at least 3 independent classes of olfactory receptor sites. Our data suggest that bile acids are discriminated by olfaction in rainbow trout, supporting the possibility that these compounds...
Tipo: Journal article
Palavras-chave: Bile acids; Chemical signals; Electro-olfactogram; Olfaction; Pheromones; Rainbow trout.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100005

LA CIENCIA EN CHILE Y EL PROGRAMA MILENIO
Provedor de dados: 48
Autores: VALDES,GABRIEL.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100002

Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion
Provedor de dados: 48
Autores: Murillo,Andrea; Guerrero,Carlos A; Acosta,Orlando; Cardozo,Carmen A.
Normal bone remodeling is maintained by a balance between osteoclast and osteoblast activity, whereas defects in osteoclast activity affecting such balance result in metabolic bone disease. Macrophage-macrophage fusion leading to multinucleated osteoclasts being formed is still not well understood. Here we present PEG-induced fusion of macrophages from both U937/A and J774 cell lines and the induced differentiation and activation of osteoclast-like cells according to the expression of osteoclast markers such as tartrate resistant acid phosphatase (TRAP) and bone resorptive activity. PEG-induced macrophage fusion, during the non-confuent stage, signifcantly increased the osteoclastogenic activity of macrophages from cell lines compared to that of...
Tipo: Journal article
Palavras-chave: Bone resorption; Macrophage fusion; Osteoclast activity; Osteoclast differentiation; PEG; RANKL.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200009

Ciencia y Política
Provedor de dados: 48
Tipo: Journal article
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100001

Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria
Provedor de dados: 48
Autores: AIYEGORO,OLAYINKA A; AFOLAYAN,ANTHONY J; OKOH,ANTHONY I.
The effect of combinations of the crude methanolic extract of the leaves of Helichrysum pedunculatum and eight first-line antibiotics were investigated by time kill assays against a panel of bacterial strains that have been implicated in wound infections. The plant extract showed appreciable antibacterial activities against the test bacteria with zones of inhibition ranging between 18 and 27 mm, and minimum inhibitory concentrations (MICs) varying between 0.1 and 5.0 mg/ml. The MICs of the test antibiotics range between 0.001 and 0.412 mg/ml, and combination of the plant extract and the antibiotics resulted in reduction of bacterial counts by between 0 and 6.63 Log10 cfu/ml. At V2 MIC, 56.81% synergy; 43.19% indifference and no antagonism were observed,...
Tipo: Journal article
Palavras-chave: Helichrysum pedunculatum; Time kill assay; Synergism.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300007

On the Biol Res issue dedicated to F. Varela
Provedor de dados: 48
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200002

Wine grape pomace flour improves blood pressure, fasting glucose and protein damage in humans: a randomized controlled trial
Provedor de dados: 48
Autores: Urquiaga,Inés; D'Acuna,Sonia; Pérez,Druso; Dicenta,Sara; Echeverria,Guadalupe; Rigotti,Attilio; Leighton,Federico.
BACKGROUND: The Mediterranean diet is a healthy diet with positive scientific evidence of preventing chronic diseases. Bioactive components support the healthy properties of the Mediterranean diet. Antioxidants and fiber, two components of the Mediterranean diet, are key functional nutrients for healthy eating and nutrition. Wine grape pomace is a rich source of these dietary constituents and may be beneficial for human health. Our hypothesis was that the intake of red wine grape pomace flour (WGPF) prepared from red wine grapes (Cabernet Sauvignon variety) reduced the metabolic syndrome in humans. To evaluate the effect of WGPF on components of metabolic syndrome we design a 16-week longitudinal intervention study. Thirty-eight males, 30-65 years of age,...
Tipo: Journal article
Palavras-chave: Antioxidant; Dietary intervention; Fiber; Metabolic syndrome; Oxidative stress; Wine grape pomace.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100049

Anatomy of corpus callosum in prenatally malnourished rats
Provedor de dados: 48
Autores: Olivares,Ricardo; Morgan,Carlos; Pérez,Hernán; Hernández,Alejandro; Aboitiz,Francisco; Soto-Moyano,Rubén; Gil,Julio; Ortiz,Alexis; Flores,Osvaldo; Gimeno,Miguel; Laborda,Jesús.
The effect of prenatal malnutrition on the anatomy of the corpus callosum was assessed in adult rats (45-52 days old). In the prenatally malnourished animals we observed a significant reduction of the corpus callosum total area, partial areas, and perimeter, as compared with normal animals. In addition, the splenium of corpus callosum (posterior fifth) showed a significant decrease of fiber diameters in the myelinated fibers without changing density. There was also a significant decrease in diameter and a significant increase in density of unmyelinated fibers. Measurements of perimeter's fractal dimensions from sagittal sections of the brain and corpus callosum did not show significant differences between malnourished and control animals. These findings...
Tipo: Journal article
Palavras-chave: Corpus callosum; Prenatal malnutrition; Rat; Splenium.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100012

Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage
Provedor de dados: 48
Autores: Amaral,Marta G; Campos,Vinicius F; Seixas,Fabiana K; Cavalcanti,Paulo V; Selau,Lisiane P. R; Deschamps,Joao C; Collares,Tiago.
Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or liposome (Lipofectin) in an attempt to improve TMGT. Males receiving consecutive DNA complex injections were mated to normal females to obtain F0 progeny. In vivo evaluation of EGFP expression, RT-PCR and PCR were used to detect the expression and the presence of exogenous DNA in the progeny. We also evaluated possible testicular damage by histological procedures. PC R and RT-PCR analyses revealed that liposome and DMSO increased the rate of TMGT. Histological...
Tipo: Journal article
Palavras-chave: TMGT DMA (N; N-dimethylacetamide) Liposome mice transgenesis histological damage.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300003

Mitochondrial Ca2+ homeostasis in health and disease
Provedor de dados: 48
Autores: CAMPANELLA,MICHELANGELO; PINTON,PAOLO; RIZZUTO,ROSARIO.
Although it has long been known that mitochondria possess a complex molecular repertoire for accumulating and releasing Ca2+, only in recent years has a large body of data demonstrated that these organelles promptly respond to Ca2+-mediated cell stimulations. In this contribution, we will review the principles of mitochondrial Ca2+ homeostasis and its signaling role in different physiological and pathological conditions.
Tipo: Journal article
Palavras-chave: Mitochondria; Calcium; Apoptosis; Bcl-2; Viral proteins.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400022

Lucilia sericata strain from Colombia: Experimental Colonization, Life Tables and Evaluation of Two Artifcial Diets of the Blowfy Lucilia sericata (Meigen) (Diptera: Calliphoridae), Bogotá, Colombia Strain
Provedor de dados: 48
Autores: Rueda,Luis C; Ortega,Luis G; Segura,Nidya A; Acero,Víctor M; Bello,Felio.
The objective of this work was to establish, under experimental laboratory conditions, a colony of Lucilia sericata, Bogotá-Colombia strain, to build life tables and evaluate two artifcial diets. This blowfy is frequently used in postmortem interval studies and in injury treatment. The parental adult insects collected in Bogotá were maintained in cages at 22°C±1 average temperature, 60%±5 relative humidity and 12 h photoperiodicity. The blowfies were fed on two artifcial diets that were evaluated over seven continuous generations. Reproductive and population parameters were assessed. The life cycle of the species was expressed in the number of days of the different stages: egg = 0.8±0.1, larvae I = 1.1±0.02, larvae II = 1.94±0.16, larvae III = 3.5±0.54,...
Tipo: Journal article
Palavras-chave: Lucilia sericata; Diet; Fecundity; Life cycle; Life table.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200008

Statistical studies on anatomical modifications in the radicle and hypocotyl of cotton induced by NaCl
Provedor de dados: 48
Autores: CASENAVE,ESTER C; DEGANO,CLAUDIA AM; TOSELLI,MARIA E; CATAN,ESTELA A.
Salinity affects extensive arid and semiarid areas all over the world, producing diminished yields of many crops. Even though there is a variety of research and reviews related to physiological and anatomical modifications produced in salinity-tolerant species, today there are different points of view in relation to this topic, and especially due to technical limitations, there are few articles in which anatomical modifications have been quantified. The aim of this research is to quantitatively and statistically evaluate the modifications that are produced in radicles and hypocotyles of cotton seedlings growing in NaCl concentrations that affect the early ontogenetic stages of this crop. Germination of two varieties of cotton at increasing concentrations...
Tipo: Journal article
Palavras-chave: Anatomical changes; Cotton; Germination; Growth; Salinity.
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400008

Antioxidant, antimicrobial, toxicity and analgesic properties of ethanol extract of Solena amplexicaulis root
Provedor de dados: 48
Autores: Kabir,Md Golam; Rahman,Md Monsor; Ahmed,Nazim Uddin; Fakruddin,Md; Islam,Saiful; Mazumdar,Reaz Mohammad.
BACKGROUND: This study was subjected to investigate different pharmacological properties of ethanol extract ofSolena amplexicaulis root. RESULTS: The extract contains flavonoid, alkaloid, saponin and steroid compounds. The extract exhibited excellent antioxidant activity in DPPH radical scavenging activity. The extract also showed potent activity in brine shrimp lethality bioassay. The LC50 value was found to 44.677 µg/ml. The extract showed better anti-bacterial activity against gram-negative bacteria. In antifungal assay, the maximum 79.31% of anti-mycotic activity was observed against Aspergillus ochraceus while minimum 44.2% against Rhizopus oryzae. MIC value ranged between 1500 - 3000 µg/ml. The extract was found moderately toxic with a 24-hr LD50...
Tipo: Journal article
Palavras-chave: Solena amplexicaulis; Antioxidant; Antimicrobial; Acute toxicity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100036

Planes de Desarrollo Gubernamentales en Ciencia y Tecnología:: ¿Promesas o Realidades?
Provedor de dados: 48
Autores: Jerez,Carlos A.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300001

Toll-like Receptors are Key Participants in Innate Immune Responses
Provedor de dados: 48
Autores: ARANCIBIA,SERGIO A; BELTRÁN,CAROLL J; AGUIRRE,ISABEL M; SILVA,PAULINA; PERALTA,ALEXIS L; MALINARICH,FRANO; HERMOSO,MARCELA A.
During an infection, one of the principal challenges for the host is to detect the pathogen and activate a rapid defensive response. The Toll-like family of receptors (TLRs), among other pattern recognition receptors (PRR), performs this detection process in vertebrate and invertebrate organisms. These type I transmembrane receptors identify microbial conserved structures or pathogen-associated molecular patterns (PAMPs). Recognition of microbial components by TLRs initiates signaling transduction pathways that induce gene expression. These gene products regulate innate immune responses and further develop an antigen-specific acquired immunity. TLR signaling pathways are regulated by intracellular adaptor molecules, such as MyD88, TIRAP/Mal, between others...
Tipo: Journal article
Palavras-chave: Toll-like receptors; Innate Immunity; Cytokines; MyD88; NFkB.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200001

The Circadian Timing System: Making Sense of day/night gene expression
Provedor de dados: 48
Autores: RICHTER,HANS G; TORRES-FARFÁN,CLAUDIA; ROJAS-GARCÍA,PEDRO P; CAMPINO,CARMEN; TORREALBA,FERNANDO; SERÓN-FERRÉ,MARÍA.
The circadian time-keeping system ensures predictive adaptation of individuals to the reproducible 24-h day/night alternations of our planet by generating the 24-h (circadian) rhythms found in hormone release and cardiovascular, biophysical and behavioral functions, and others. In mammals, the master clock resides in the suprachiasmatic nucleus (SCN) of the hypothalamus. The molecular events determining the functional oscillation of the SCN neurons with a period of 24-h involve recurrent expression of several clock proteins that interact in complex transcription/translation feedback loops. In mammals, a glutamatergic monosynaptic pathway originating from the retina regulates the clock gene expression pattern in the SCN neurons, synchronizing them to the...
Tipo: Journal article
Palavras-chave: Biological rhythms; Circadian timing system; Clock genes; Melatonin.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100003

Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens
Provedor de dados: 48
Autores: Toledo,Victor; Ramírez,Galia; Valck,Carolina; López,Nandy; Ribeiro,Carolina H; Maldonado,Ismael; Aguilar,Lorena; Lemus,David; Ferreira,Arturo.
Angiogenesis is a complex multi-step process of neovascularization arising from preexisting blood vessels whose generation is regulated by pro- and anti-angiogenic factors. Both Trypanosoma cruzi calreticulin (TcCRT) and its human counterpart (HuCRT) are antiangiogenic. This is the first report where the TcCRT and HuCRT anti-angiogenic properties are compared in vivo. In the chick embryonic chorioallantoid membrane assay (CAM) and at equimolar concentrations, TcCRT displayed significantly higher antiangiogenic activities than its human counterpart. LPS had marginal effects at the concentrations present in the recombinant protein preparations and the TcCRT antiangiogenic effects were largely inhibited by specific polyclonal antibodies, thus, reinforcing the...
Tipo: Journal article
Palavras-chave: Calreticulin; T. cruzi; Anti-angiogenesis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300004

Assessment of allelopathic properties of Aloe ferox Mill. on turnip, beetroot and carrot
Provedor de dados: 48
Autores: Arowosegbe,Sunday; Afolayan,Anthony J.
Turnip (Brassica rapa var. rapa L.), beetroot (Beta vulgaris L.) and carrot (Daucus carota L.) are common vegetables in South Africa. The allelopathic potential of aqueous leaf and root extracts of Aloe ferox Mill.- a highly valued medicinal plant- was evaluated against seed germination and seedling growth of the three vegetables in Petri dish experiments. The extracts were tested at concentrations of 2, 4, 6, 8, and 10 mg/mL. Leaf extract concentrations above 4 mg/mL inhibited the germination of all the crops, while the root extract had no significant effect on germination irrespective of concentration. Interestingly, the lowest concentration of leaf extract stimulated root length elongation of beetroot by 31.71%. Other concentrations significantly...
Tipo: Journal article
Palavras-chave: Allelopathy; Aloe ferox; Aqueous extracts; Intercrops; Vegetable crops.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400006

Professor Jaime Alvarez: don Quixote de la Mancha at the University
Provedor de dados: 48
Autores: ZAPATA,PATRICIO.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200013

Anti-inflammatory and redox-protective activities of citronellal
Provedor de dados: 48
Autores: Melo,Mônica S; Guimarães,Adriana G; Santana,Michele F; Siqueira,Rosana S; De Lima,Amanda Do Carmo B; Dias,Antonio S; Santos,Márcio Roberto V; Onofre,Alexandre S.C.; Quintans,Jullyana S.S.; De Sousa,Damião P; Almeida,Jackson R.G.S.; Estevam,Charles S; Araujo,Brancilene S; Quintans-Júnior,Lucindo J.
The anti-inflammatory and redox protective effects of the citronellal (CT) were evaluated using in vivo and in vitro tests. Intraperitoneal (i.p.) administration of CT (50, 100, and 200 mg/kg) inhibited (p &lt; 0.05) the carrageenan-induced leukocyte migration to the peritoneal cavity. Additionally, the carrageenan- and arachidonic acid-induced rat hind paw edema was significantly inhibited (p &lt; 0.05) by i.p. administration of 100 and 200 mg/kg of the compound. When the redox activity was evaluated, CT (200 mg/kg) significantly reduced hepatic lipoperoxidation (p &lt; 0.001), as well as oxidation of plasmatic (p &lt; 0.05) and hepatic (p &lt; 0.01) proteins. The results of the present study support the hypothesis that CT possesses anti-inflammatory and...
Tipo: Journal article
Palavras-chave: Monoterpene; Citronellal; Anti-inflammatory; Redox.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400008

Novel model of calcium and inositol 1,4,5-trisphosphate regulation of InsP3 receptor channel gating in native endoplasmic reticulum
Provedor de dados: 48
Autores: Foskett,J. Kevin; Mak,D.-O. Daniel.
The InsP3R Ca2+-release channel has biphasic dependence on cytoplasmic free Ca2+ concentration ([Ca2+]i). InsP3 activates gating primarily by reducing high [Ca2+]i inhibition. To determine whether relieving Ca2+ inhibition is sufficient for activation, we examined single-channels in low [Ca2+]i in the absence of InsP3 by patch clamping isolated Xenopus oocyte nuclei. For both endogenous Xenopus type 1 and recombinant rat type 3 InsP3R channels, spontaneous InsP3-independent activities with low open probability Po (~ 0.03) were observed in [Ca2+]i &lt; 5 nM, whereas none were observed in 25 nM Ca2+. These results establish the half-maximal inhibitory [Ca2+]i in the absence of InsP3 and demonstrate that the channel can be active when all of its...
Tipo: Journal article
Palavras-chave: InsP3 receptor; Calcium release channel; Calcium; Ion channel; Model.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400004

Differential roles for pathogenicity islands SPI-13 and SPI-8 in the interaction of Salmonella Enteritidis and Salmonella Typhi with murine and human macrophages
Provedor de dados: 48
Autores: Espinoza,Rodrigo A.; Silva&amp;#8209;Valenzuela,Cecilia A.; Amaya,Fernando A.; Urrutia,Ítalo M.; Contreras,Inés; Santiviago,Carlos A.
Abstract  Background Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interaction with macrophages is a critical step in Salmonella pathogenicity, in this study we investigated the role played by SPI-13 and SPI-8 in the interaction of S. Enteritidis and S. Typhi with cultured murine (RAW264.7) and human (THP-1) macrophages.  Results  Our results showed that SPI-13 was required for internalization of S. Enteritidis in murine but not human macrophages. On the other hand, SPI-8 was not required for the interaction of S. Typhi...
Tipo: Journal article
Palavras-chave: Salmonella; Enteritidis; Typhi; SPI-13; SPI-8; Macrophages; RAW264.7; THP-1.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100402

Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats
Provedor de dados: 48
Autores: Chen,Xiao-Guang; Xu,Cun-Shuan.
BACKGROUND: Liver regeneration (LR) after 2/3 partial hepatectomy (PH) is one of the most studied models of cell, organ, and tissue regeneration. Although the transcriptional profile analysis of regenerating liver has been carried out by many reserachers, the dynamic protein expression profile during LR has been rarely reported up to date. Therefore, this study aims to detect the global proteomic profile of the regenerating rat liver following 2/3 hepatectomy, thereby gaining some insights into hepatic regeneration mechanism. RESULTS: Protein samples extracted from the sham-operated and the regenerating rat livers at 6, 12, 24, 72, 120 and 168 h after PH were separated by IEF/SDS-PAGE and then analyzed by MALDI-TOF/TOF mass spectrometry. Compared to...
Tipo: Journal article
Palavras-chave: Partial hepatectomy; Rat liver regeneration; Proteomic; MALDI TOF/TOF MS.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100053

Plant regeneration of natural tetraploid Trifolium  Hum pratense L
Provedor de dados: 48
Autores: ÇÖLGEÇEN,HATICE; TOKER,M CIHAT.
The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation has been observed on explants initiated from apical meristem placed on PC-L2 medium that includes 2 mg dm-3 BAP and 1 mg dm-3 NAA. 94.4% of the shoots originated from calli were rooted on PC-L2 medium with 1 mg dm-3 NAA. In vitro organogénesis has been accomplished in the natural tetraploid T. pratense regenerated plants successively transferred to the field
Tipo: Journal article
Palavras-chave: Red clover (Trifolium pratense L.); Fabaceae; Organogenesis; Plant regeneration.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100004

Distinctive Effects of Red Wine and Diet on Haemostatic Cardiovascular Risk Factors
Provedor de dados: 48
Autores: MEZZANO,DIEGO.
The aim of this study was to compare the effects of Mediterranean-type diet (MD), high-fat diet (HFD), and red wine supplementation on plasma concentration of emergent haemostatic cardiovascular risk factors (HCVRF) and on variables of primary haemostasis (bleeding time, plasma von Willebrand factor and platelet aggregation/secretion). In a controlled prospective intervention study, two groups (21 healthy males each) received either MD or HFD during 90 days. Between days 30-60, both diets were supplemented with 240 ml/day of red wine. After adjusting by baseline values, MD was associated with: lower plasma fibrinogen (p =0.03), factor VIIc (p=0.034) and factor VIIIc (p=0.0057); higher levels of protein S (p=0.013); longer bleeding time (p=0.017); and...
Tipo: Journal article
Palavras-chave: Bleeding time; Cardiovascular risk factors; Diet; Haemostasis; Platelet function; Wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200007

Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats?
Provedor de dados: 48
Autores: Da,Yun&amp;#8209;Meng; Niu,Kai&amp;#8209;Yu; Liu,Shu&amp;#8209;Ya; Wang,Ke; Wang,Wen&amp;#8209;Juan; Jia,Jing; Qin,Li&amp;#8209;Hua; Bai,Wen&amp;#8209;Pei.
Abstract  Background Cimicifuga racemosa is one of the herbs used for the treatment of climacteric syndrome, and it has been cited as an alternative therapy to estrogen. Apart from hectic fevers, dyspareunia and so on, dry mouth also increase significantly after menopause. It has not yet been reported whether C. racemosa has any impact on the sublingual gland, which may relate to dry mouth. In an attempt to determine this, we have compared the effects of estrogen and C. racemosa on the sublingual gland of ovariectomized rats.  Results  HE staining showed that the acinar cell area had contracted and that the intercellular spaces were broadened in the OVX (ovariectomized rats) group, while treatment with estradiol (E2) and iCR (isopropanolic extract of C....
Tipo: Journal article
Palavras-chave: Estradiol; Cimicifuga racemosa; Sublingual gland; Subcellular structure; Caspase-3.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100209

Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains
Provedor de dados: 48
Autores: Keyhanmanesh,Rana; Boskabady,Mohammad H.
In previous studies, the relaxant effect of Tymus vulgaris has been demonstrated on guinea pig tracheal chains. Therefore, in the present study, the relaxant effects of n-hexane, dichloromethane, methanol and aqueous fractions of Tymus vulgaris on tracheal chains of guinea pigs were examined. The relaxant effects of four cumulative concentrations of each fraction (0.4, 0.8, 1.2 and 1.6 g%) in comparison to saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mM) were examined for their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 ìÌ methacholine (group 2, n = 7 for each group). In group 1, all concentrations of the n-hexane fraction and theophylline and three...
Tipo: Journal article
Palavras-chave: Tymus vulgaris; Fractions; Relaxant effects; Guinea pig; Trachea.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100009

Two alternative models concerning the perialveolar microcirculation in mammalian lungs
Provedor de dados: 48
Autores: GÜNTHER,BRUNO; MORGADO,ENRIQUE; COCIÑA,MANUELA.
Despite the fact that the concept of sheet-flow in the pulmonary microcirculation of mammals was introduced more than three decades ago, the capillary circulatory model still prevails in the physiological literature. Since cardiac output is identical in the systemic and in pulmonary circulations, it is noteworthy that in the former, the resulting arterial pressure is five times higher than that of the latter, which means that the corresponding microcirculations must be radically different. The present study addresses this problem from both morphological and physiological perspectives.
Tipo: Journal article
Palavras-chave: Arterial pressures; Capillaries; Pulmonary microcirculation; Sheet flow; Systemic circulation.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100007

XLIX REUNIÓN ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CHILE
Provedor de dados: 48
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000400002

On-line analysis of biosignals for the automation of total and specific sleep deprivation in the rat
Provedor de dados: 48
Autores: VIVALDI,ENNIO A; BASSI,ALEJANDRO; ESTRADA,JORGE; GARRIDO,IGNACIO; DÍAZ,JAVIER; OCAMPO-GARCÉS,ADRIÁN.
A computer-based system that automates sleep studies, including sleep deprivation paradigms, is described. The system allows for total or REM-specific sleep deprivation and is based on a reliable, fast-responding, on-line state detection algorithm linked to a dependable intervention device. Behavioral state detection is achieved by dimensión reduction of short-term EEG power spectrum. Interventions are made by serial outputs to servomotors that move a cage with different patterns and variable intensity. The system can adapt itself to individual characteristics and to changes in recording conditions. Customized protocols can be designed by defining the states or stages to be deprived, including scheduling temporal patterns. A detailed analysis of the...
Tipo: Journal article
Palavras-chave: Sleep; Sleep deprivation; Biosignal analysis; Digital control; Automation.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400009

Iron at the center of ferritin, metal/oxygen homeostasis and novel dietary strategies
Provedor de dados: 48
Autores: LIU,X; HINTZE,K; LONNERDAL,B; THEIL,EC.
Bioiron _ central to respiration, photosynthesis and DNA synthesis and complicated by radical chemistry with oxygen _ depends on ferritin, the super family of protein nanocages (maxi-ferritins in humans, animals, plants and bacteria, and mini-ferritins, also called DPS proteins, in bacteria) for iron and oxygen control. Regulation of ferritin synthesis, best studied in animals, uses DNA transcription and mRNA translation check points. Ferritin is a member of both the "oxidant stress response" gene family that includes thioredoxin reductase and quinine reductase, and a member of the iron responsive gene family that includes ferroportin and mt-aconitase ferritin DNA regulation responds preferentially to oxidant response inducers and ferritin mRNA to iron...
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100018

MicroRNA-98 inhibits the cell proliferation of human hypertrophic scar fibroblasts via targeting Col1A1
Provedor de dados: 48
Autores: Bi,Sheng; Chai,Linlin; Yuan,Xi; Cao,Chuan; Li,Shirong.
Abstract  Background  Hypertrophic scarring (HS) is a severe disease, and results from unusual wound healing. Col1A1 could promote the hypertrophic scar formation, and the expression of Col1A1 in HS tissue was markedly higher than that in the normal. In present study, we aimed to identify miRNAs as post-transcriptional regulators of Col1A1 in HS.  Methods  MicroRNA-98 was selected as the key miRNA comprised in HS. The mRNA levels of miR-98 in HS tissues and the matched normal skin tissues were determined by qRT-PCR. MTT and flow cytometry were used to determine the influence of miR-98 on cell proliferation and apoptosis of HSFBs, respectively. Col1A1 was found to be the target gene of miR-98 using luciferase reporter assay. Luciferase assay was performed...
Tipo: Journal article
Palavras-chave: MiR-98; Col1A1; Apoptosis; Proliferation; HSFBs.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100215

Proton NMR characterization of intact primary and metastatic melanoma cells in 2D &amp;amp; 3D cultures
Provedor de dados: 48
Autores: Ramachandran,Gokula Krishnan; Yeow,Chen Hua.
Abstract  Objective  To characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures.  Methods  Primary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. These cells were characterized using proton NMR spectrometry, and the qualitative chemical shifts markers were identified and discussed.  Results  In monolayer culture (2D), we observed one qualitative chemical shift marker for primary melanoma cells. In spheroid cultures (3D), we observed nine significant chemical shifts, of which eight markers were specific for primary melanoma spheroids, whereas the other one marker was specific to metastatic melanoma...
Tipo: Journal article
Palavras-chave: Nuclear magnetic resonance; Proton magnetic resonance spectrometry; Melanoma; Cancer.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100208

To be or not to be&amp;#8230; Nicotinic acid adenine dinucleotide phosphate (NAADP): A new intracellular second messenger?
Provedor de dados: 48
Autores: NUNES CHINI,EDUARDO.
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent activator of intracellular Ca2+ release in several vertebrate and invertebrate systems. The role of the NAADP system in physiologicalprocesses is being extensively investigated at the present time. The NAADP receptor and its associated Ca2+ pool have been hypothesized to be important in several physiologicalprocesses including fertilization, T cell activation, and pancreaticsecretion. However, whether NAADP is a new second messenger ora tool for the discovery of a new Ca2+ channel is still an unansweredquestion. Research developed over the last two years has provided some important clues to whether NAADP is or not a physiological cellular messenger. In this short review, I will discuss some...
Tipo: Journal article
Palavras-chave: NAADP; Ca2+; Channels; CADPR; Intracellular; Second messenger.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400009

Phytochemical Profile and Qualification of Biological Activity of an Isolated Fraction of Bellis perennis
Provedor de dados: 48
Autores: Costa Marques,Thiago H; Santos De Melo,Cassio H; Fonseca De Carvalho,Rusbene B; Costa,Luciana M; De Souza,Alexandre A; David,Jorge M; De Lima David,Juceni P; De Freitas,Rivelilson M.
This study describes the isolation and identification of apigenin-7-O-ghicopyranoside, a flavonoid isolated from the flowers of Bellis perennis L., Asteraceae, an species with a broad spectrum of biological activities. The in vitro antioxidant activity and the inhibition of the enzyme acetylcholinesterase were evaluated. The flavonoid showed strong in vitro antioxidant potential, because of the capacity of removal of hydroxyl radicals and nitric oxide, and also prevented the formation of thiobarbituric acid-reactive substances. These parameters were inhibited at the highest concentration of ApG at rates of 77.7%, 72% and 73.4%, respectively, in addition to inhibiting acetylcholinesterase, suggesting potential use in the treatment of neurodegenerative...
Tipo: Journal article
Palavras-chave: Acetylcholinesterase; Antioxidant; Bellis perennis; Flavonoid.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300002

Plant Polyphenol Antioxidants and Oxidative Stress
Provedor de dados: 48
Autores: URQUIAGA,INES; LEIGHTON,FEDERICO.
In recent years there has been a remarkable increment in scientific articles dealing with oxidative stress. Several reasons justify this trend: knowledge about reactive oxygen and nitrogen species metabolism; definition of markers for oxidative damage; evidence linking chronic diseases and oxidative stress; identification of flavonoids and other dietary polyphenol antioxidants present in plant foods as bioactive molecules; and data supporting the idea that health benefits associated with fruits, vegetables and red wine in the diet are probably linked to the polyphenol antioxidants they contain.In this review we examine some of the evidence linking chronic diseases and oxidative stress, the distribution and basic structure of plant polyphenol antioxidants,...
Tipo: Journal article
Palavras-chave: Oxidative stress; Antioxidant; Plant polyphenol; Flavonoid; Chronic diseases; Diet.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200004

Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
Provedor de dados: 48
Autores: ACOSTA-RIVERO,NELSON; POUTOU,JOANNA; ÁLVAREZ-LAJONCHERE,LIZ; GUERRA,IVIS; AGUILERA,YARAIMA; MUSACCHIO,ALEXIS; RODRÍGUEZ,ARMANDO; AGUILAR,JULIO C; FALCON,VIVIANA; ÁLVAREZ-OBREGON,JULIO C; SORIA,YORDANKA; TORRES,DINORAH; LINARES,MARBELIS; PÉREZ,ÁNGEL; MORALES-GRILLO,JUAN; DUEÑAS -CARRERA,SANTIAGO.
In the present work, immunogenicity of recombinant in vitro assembled hepatitis C virus core particles, HCcAg.120-VLPs, either alone or in combination with different adjuvants was evaluated in BALB/c mice. HCcAg.120-VLPs induced high titers of anti-HCcAg.120 antibodies and virus-specific cellular immune responses. Particularly, HCcAg.120-VLPs induced specific delayed type hypersensitivity, and generated a predominant T helper 1 cytokine pro file in immunized mice. In addition, HCcAg.120-VLPs prime splenocytes proliferate in vitro against different HCcAg.120-specific peptides, depending on either the immunization route or the adjuvant used. Remarkably, immunization with HCcAg.120-VLPs/Montanide ISA888 formulation resulted in a significant control of...
Tipo: Journal article
Palavras-chave: Challenge; HCV; Immunity; Adjuvant; Capsid.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100005

Effect of neuroserpin in a neonatal hypoxic-ischemic injury model ex vivo
Provedor de dados: 48
Autores: Ma,Jiao; Yu,Dan; Tong,Yu; Mao,Meng.
Hypoxia-ischemia (HI) occurring in immature brains stimulates the expression of tissue-type plasminogen activator (tPA). Neuroserpin is a selected inhibitor of tPA in the central nerves system. However, the role that neuroserpin plays and the possible mechanisms involved during neonatal HI are poorly defined. In this study, an oxygen-glucose deprivation and reoxygenation (OGD/R) model was generated with cultured rat cortical neurons mimicking neonatal HI injury ex vivo, and an acute neuronal excitatory injury was induced by exposure to a high concentration of N-methyl-D-aspartic acid (NMDA). Cells received either neuroserpin or MK-801, an antagonist of the NMDA receptor, during OGD/R, and were incubated with or without neuroserpin after NMDA exposure. Cell...
Tipo: Journal article
Palavras-chave: Excitotoxicity; Hypoxia-ischemia; Neuroserpin; N-methyl-D-aspartic; Tissue-type plasminogen activator.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400005

Suppression of miR-221 inhibits glioma cells proliferation and invasion via targeting SEMA3B
Provedor de dados: 48
Autores: Cai,Guilan; Qiao,Shanshan; Chen,Kui.
BACKGROUND: Gliomas are the most common primary tumors in the central nervous system. Due to complicated signaling pathways involved in glioma progression, effective targets for treatment and biomarkers for prognosis prediction are still scant. RESULTS: In this study we revealed that a new microRNA (miR), the miR-221, was highly expressed in the glioma cells, and suppression of miR-221 resulted in decreased cellular proliferation, migration, and invasion in glioma cells. Mechanistic experiments validated that miR-221 participates in regulating glioma cells proliferation and invasion via suppression of a direct target gene, the Semaphorin 3B (SEMA3B). The rescue experiment with miR-221 and SEMA3B both knockdown results in significant reversion of miR-221...
Tipo: Journal article
Palavras-chave: MiR-221; Glioma; SEMA3B; Cancer metastasis.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100037

Analysis of chlorophyll fluorescence reveals stage specific patterns of chloroplast-containing cells during Arabidopsis embryogenesis
Provedor de dados: 48
Autores: TEJOS,RICARDO I; MERCADO,ANA V; MEISEL,LEE A.
The basic body plan of a plant is established early in embryogenesis when cells differentiate, giving rise to the apical and basal regions of the embryo. Using chlorophyll fluorescence as a marker for chloroplasts, we have detected specific patterns of chloroplast-containing cells at specific stages of embryogenesis. Non-randomly distributed chloroplast-containing cells are seen as early as the globular stage of embryogenesis in Arabidopsis. In the heart stage of embryogenesis, chloroplast containing cells are detected in epidermal cells as well as a central region of the heart stage embryo, forming a triangular septum of chloroplast-containing cells that divides the embryo into three equal sectors. Torpedo stage embryos have chloroplast-containing...
Tipo: Journal article
Palavras-chave: Chloroplast; Plastid differentiation; Embryogenesis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100012

Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
Provedor de dados: 48
Autores: Votto,Ana P. S; Domingues,Beatriz S; de Souza,Michele M; da Silva Júnior,Flavio M. R; Caldas,Sergiane S; Filgueira,Daza M. V. B; Clementin,Rosilene M; Primel,Ednei G; Vallochi,Adriana L; Furlong,Eliana B; Trindade,Gilma S.
Onion (Allium cepa) is being studied as a potential anticancer agent, but little is known regarding its effect in multidrug resistance (MDR) cells. In this work, the cytotoxicity of crude onion extract (OE) and fractioned extract (aqueous, methanolic and ethyl acetate), as well as some onion compounds (quercetin and propyl disulfide) were evaluated in Lucena MDR human erythroleukemic and its K562 parental cell line. The capacity of OE to induce apoptosis and/or necrosis in these cells, the possible participation of oxidative stress and DNA damage were also assessed. Similar sensitivities were obtained for both tumoral cells, however only OE caused significant effects in the cells. In K562 cells, a significant increase of apoptosis was verified while the...
Tipo: Journal article
Palavras-chave: Antioxidant; Apoptosis and/or necrosis; DNA damage; MDR phenotype; Onion (Allium cepa); Tumoral cell.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400007

DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis
Provedor de dados: 48
Autores: BERNAL,CAROLINA; VARGAS,MACARENA; OSSANDÓN,FRANCISCO; SANTIBÁÑEZ,EUDOCIA; URRUTIA,JULIO; LUENGO,VÍCTOR; ZAVALA,LUIS F; BACKHOUSE,CLAUDIA; PALMA,MARIANA; ARGANDOÑA,JORGE; AGUAYO,FRANCISCO; CORVALÁN,ALEJANDRO.
Diffuse type gastric carcinoma is the most aggressive type of gastric cáncer. This type of tumor is not preceded by precancerous changes and is associated with early-onset and hereditary syndromes. To test the hypothesis that DNA methylation profile would be useful for molecular classification of the diffuse type gastric carcinoma, DNA methylation patterns of the CpG Island of 17 genes were studied in 104 cases and 47 normal adjacent gastric mucosa by Methylation-specific PCR, Immunohistochemistry and Hierarchical clustering analysis. The most frequent methylated genes were FHIT, E-cadherin, BRCA1 and APC (&gt;50%), followed by pl4, pl6, pl5, p73, MGMT and SEMA3B (20-49%). Hierarchical clustering analysis reveáis four groups with different clinical...
Tipo: Journal article
Palavras-chave: Gastric cáncer; Diffuse type; Early-onset; BRCA-1.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300007

Nkx-2.3 gene in mouse epidemial maturation
Provedor de dados: 48
Autores: MA,CHANG M.
In higher vertebrates, from amphibians to humans, epidemial maturation is a conserved developmental process. Using adult epidemial tissue and an established keratinocyte cell line, the mouse Nkx-2.3 homeobox gene was demonstrated, for the first time, to be expressed in mouse epidermal keratinocytes. Under the normal culture condition, the spontaneous aggregation phenomenon, a common initiation step of ES cell differentiation, and the induction of mouse adult K1 keratin, a marker of mature epidermal keratinocytes, were both observed in vitro when the Xenopus Nkx-2.3 gene was stably transfected into a mouse pluripotent P19 EC cell line. The induction of mouse K1 keratin by using its Xenopus orthologous gene in the mouse P19 cell implies that Nkx-2.3 may play...
Tipo: Journal article
Palavras-chave: Nkx-2.3; Epidermis; Homeobox gene; Skin maturation; Differentiation.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300001

Sobre el deber académico
Provedor de dados: 48
Autores: Jaksic,Fabian.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300001

Right but basically wrong: Comments on Canales et al. 'A Critical Assessment of the Consciousness by Synchrony Hypothesis'
Provedor de dados: 48
Autores: RODRÍGUEZ,EUGENIO; CORTÉS,JOSÉ.
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500013

Synaptic configuration of quadrivalents and their association with the XY bivalent in spermatocytes of Robertsonian heterozygotes of Mus domesticus
Provedor de dados: 48
Autores: Berríos,Soledad; Fernández&amp;#8211;Donoso,Raúl; Ayarza,Eliana.
Abstract  Background The nuclear architecture of meiotic prophase spermatocytes is based on higher&amp;#8211;order patterns of spatial associations among chromosomal domains and consequently is prone to modification by chromosomal rearrangements. We have shown that nuclear architecture is modified in spermatocytes of Robertsonian (Rb) homozygotes of Mus domesticus. In this study we analyse the synaptic configuration of the quadrivalents formed in the meiotic pro&amp;#8211; phase of spermatocytes of mice double heterozygotes for the dependent Rb chromosomes: Rbs 11.16 and 16.17.  Results Electron microscope spreads of 60 pachytene spermatocytes from four animals of Mus domesticus 2n = 38 were studied and their respective quadrivalents analysed in detail....
Tipo: Journal article
Palavras-chave: Quadrivalents; Robertsonian chromosomes; Mouse spermatocytes; Nucleoli; Nuclear architecture.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100405

Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla
Provedor de dados: 48
Autores: VINET,RAÚL; CORTÉS,MAGDALENA; LUXORO,MARIO; DELPIANO,MARCO A.
Endothelial cells are directly involved in many functions of the cardiovascular system by regulating blood flow and blood pressure through Ca2+ dependent exocitosis of vasoactive compounds. Using the Ca2+ indicator Fluo-3 and the patch-clamp technique, we show that bovine adrenal medulla capillary endothelial cells (B AMCECs) respond to acetylcholine (ACh) with a cytosolic Ca2+ increase and depolarization of the membrane potential (20.3±0.9 mV; n=23). The increase in cytosolic Ca2+ induced by 10µM ACh was mimicked by the same concentration of nicotine but not by muscarine and was blocked by 100 µM of hexamethonium. On the other hand, the increase in cytosolic Ca2+ could be depressed by nifedipine (0.01 -100 µM) or withdrawal of extracellular Ca2+. Taken...
Tipo: Journal article
Palavras-chave: Capillary; Endothelial cells; Intracellular Ca2+; Nicotinic receptor; Voltage-activated Ca2+ channels.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100012

Omissions in the synthetic theory of evolution
Provedor de dados: 48
Autores: Frías L,Daniel.
The Synthetic Theory of Evolution is the most unifying theory of life science. This theory has dominated scientific thought in explaining the mechanisms involved in speciation. However, there are some omissions that have delayed the understanding of some aspects of the mechanisms of organic evolution, principally: 1) the bridge between somatic and germinal cells, especially in some phylum of invertebrates and vertebrates; 2) horizontal genetic transferences and the importance of viruses in host adaptation and evolution; 3) the role of non-coding DNA and non-transcriptional genes; 4) homeotic evolution and the limitations of gradual evolution; and 5) excessive emphasis on extrinsic barriers to animal speciation. This paper reviews each of these topics in an...
Tipo: Journal article
Palavras-chave: Evolution non-transcriptional genes viruses homeosis; Epigénesis imprinting neo-Lamarckism sympatric speciation.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300006

Antioxidant activity of extract from Polygonum aviculare L.
Provedor de dados: 48
Autores: HSU,CHIN-YUAN.
Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum aviculare L. (Polygonaceae) has antioxidant activity is unknown. In this study, dried Polygonum aviculare L. was extracted by ethanol, and the extract was lyophilized. The antioxidant activities of extract powder were examined by free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays. The results show that the IC50 value of Polygonum aviculare L. extract is 50 µg/ml in free radical scavenging assays, 0.8 µg/ml in superoxide radical scavenging...
Tipo: Journal article
Palavras-chave: Antioxidant activity; Free radical; Phenolics; Lipid peroxidation; DNA damage Polygonum aviculare L.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200010

Volatile Organic Compounds Produced by Human Skin Cells
Provedor de dados: 48
Autores: ACEVEDO,CRISTIAN A; SÁNCHEZ,ELIZABETH Y; REYES,JUAN G; YOUNG,MANUEL E.
Skin produces volatile organic compounds (VOCs) released to the environment with emission patterns characteristic of climatic conditions. It could be thought that these compounds are intermediaries in cell metabolism, since many intermediaries of metabolic pathways have a volatile potential. In this work, using gas chromatography, we answered the question of whether VOC profiles of primary cultures of human dermal fibroblasts were affected by the type of culture conditions. VOCs were determined for different types of culture, finding significant differences between skin cells grown in classical monolayer culture -2D- compared with 3D matrix immobilized cultures. This indicates that VOC profiles could provide information on the physiological state of skin...
Tipo: Journal article
Palavras-chave: GC/MS; Skin cells; SPME; Volatile organic compounds.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400009

Salinity-induced changes in the morphology and major mineral nutrient composition of purslane (Portulaca oleracea L.) accessions
Provedor de dados: 48
Autores: Alam,Md. Amirul; Juraimi,Abdul Shukor; Rafii,M. Y.; Hamid,Azizah Abdul; Aslani,Farzad; Hakim,M. A.
This study was undertaken to determine the effects of varied salinity regimes on the morphological traits (plant height, number of leaves, number of flowers, fresh and dry weight) and major mineral composition of 13 selected purslane accessions. Most of the morphological traits measured were reduced at varied salinity levels (0.0, 8, 16, 24 and 32 dS m-1), but plant height was found to increase in Ac1 at 16 dS m-1 salinity, and Ac13 was the most affected accession. The highest reductions in the number of leaves and number of flowers were recorded in Ac13 at 32 dS m-1 salinity compared to the control. The highest fresh and dry weight reductions were noted in Ac8 and Ac6, respectively, at 32 dS m-1 salinity, whereas the highest increase in both fresh and dry...
Tipo: Journal article
Palavras-chave: Purslane (Portulaca oleracea L.); NaCl; Salinity; Morphology; Mineral compositions.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100024

Functional implications of RyR-DHPR relationships in skeletal and cardiac muscles
Provedor de dados: 48
Autores: FRANZINI-ARMSTRONG,CLARA.
Dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs) interact during EC coupling within calcium release units, CRUs. The location of the two channels and their positioning are related to their role in EC coupling. als DHPR and RyR1 of skeletal muscle form interlocked arrays. Groups of four DHPRs (forming a tetrad) are located on alternate RyR1s. This association provides the structural framework for reciprocal signaling between the two channels. RyR3 are present in some skeletal muscles in association with RyR1 and in ratios up to 1:1. RyR3 neither induce formation of tetrads by DHPRs nor sustain EC coupling. RyR3 are located in a parajunctional position, in proximity of the RyR1-DHPR complexes, and they may be indirectly activated by calcium...
Tipo: Journal article
Palavras-chave: Calcium release units; Dihydropyridine receptors; Ryanodine receptors; Transverse tubules; Sarcoplasmic reticulum.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400003

Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer's and other neurological disorders
Provedor de dados: 48
Autores: Kamal,Zul; Ullah,Farhat; Ayaz,Muhammad; Sadiq,Abdul; Ahmad,Sajjad; Zeb,Anwar; Hussain,Abid; Imran,Muhammad.
BACKGROUND: Atriplex laciniata L. was investigated for phenolic, flavonoid contents, antioxidant, anticholinesterase activities, in an attempt to explore its effectiveness in Alzheimer's and other neurological disorders. Plant crude methanolic extract (Al.MeF), subsequent fractions; n-hexane (Al.HxF), chloroform (Al.CfF), ethyl acetate (Al.EaF), aqueous (Al.WtF), Saponins (Al.SPF) and Flavonoids (Al.FLVF) were investigated for DPPH, ABTS and H2O2 free radical scavenging activities. Further these extracts were subjected to acetylcholinesterase (AChE) &amp;amp; butyrylcholinesterase (BChE) inhibitory activities using Ellman's assay. Phenolic and Flavonoid contents were determined and expressed in mg Gallic acid GAE/g and Rutin RTE/g of samples respectively....
Tipo: Journal article
Palavras-chave: Phenolic &amp;amp; Flavonoid contents DPPH ABTS H2O2 Gallic acid IC50 and anticholinesterase.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100021

Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis
Provedor de dados: 48
Autores: SANT’ANNA,JULIANE R; MIYAMOTO,CLÁUDIA T; ROSADA,LÚCIA J; FRANCO,CLAUDINÉIA C S; KANESHIMA,EDILSON N; CASTRO-PRADO,MARIALBA A A.
The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profile. Based on pairings of nit mutants from the different isolates, five vegetative complementation groups (VCG) were identified, and barriers to the formation of heterokaryons were observed among isolates derived from the same geographic area. No complementation was observed among any of the nit mutants recovered from the isolate A, which was designed heterokaryon-self-incompatible. Based on RAPD analysis, a polymorphism was detected among the wild isolate C and...
Tipo: Journal article
Palavras-chave: Genetic variability; Heterokaryosis; Parasexual recombination; RAPD.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100007

Pesticidal and pest repellency activities of rhizomes of Drynaria quercifolia (J. Smith) against Tribolium castaneum (Herbst)
Provedor de dados: 48
Autores: Khan,Alam; Islam,Md Hedayetul; Islam,Md Ekramul; Al-Bari,Md Abdul Alim; Parvin,Mst Shahnaj; Sayeed,Mohammed Abu; Islam,Md Nurul; Haque,Md Ekramul.
BACKGROUND: Tribolium castaneum (Herbst) is a harmful pest of stored grain and flour-based products in tropical and subtropical region. In the present study, rhizome of Drynaria quercifolia (J. Smith) was evaluated for pesticidal and pest repellency activities against T. castaneum, using surface film method and filter paper disc method, respectively. In addition, activity of the isolated compound 3,4-dihydroxybenzoic acid was evaluated against the pest. RESULTS: Chloroform soluble fraction of ethanol extract of rhizome of D. quercifolia showed significant pesticidal activity at doses 0.88 to 1.77 mg/cm² and significant pest repellency activity at doses 0.94 to 0.23 mg/cm². No pesticidal and pest repellency activity was found for petroleum ether, ethyl...
Tipo: Journal article
Palavras-chave: Drynaria quercifolia Ethanol extract Methanol soluble fraction 3; 4-dihydroxybenzoic acid Tribolium castaneum.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100074

Role of regenerating gene IA expression on local invasion and survival in nasopharyngeal carcinoma
Provedor de dados: 48
Autores: Xing,Haijie; Chen,Xiangdong; Han,Yaofeng.
Abstract  Background Regenerating gene IA (REGIA) plays an important role in tissue regeneration and tumors prognosis of epithelium origin. However, the role of REGIA in nasopharyngeal carcinoma (NPC) is unclear. This study aims to investigate the expression and function of REG1A in NPC.  Results We have found that there was 63 patients with REGIA positive expression of 155 patients in this study (40.65%). The positive expression rate of REGIA was 30.50, 44.44 and 47.83% in stage T2, T3 and T4 patients, respectively. The REGIA expression was significantly difference in T2 and T4 stage tumors or T2 and T3&amp;#8211;T4 stage. The positive expression rate of REGIA was found to be higher in patients with cervical lymph node persistence than those with cervical...
Tipo: Journal article
Palavras-chave: Nasopharyngeal carcinoma; Regenerating gene IA; Invasion; Survival.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100227

Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
Provedor de dados: 48
Autores: AVILA,ANA D; CALDERÓN,CARLOS F; PEREZ,RITA M; PONS,CARMEN; PEREDA,CELIA M; ORTIZ,ANA R.
Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that...
Tipo: Journal article
Palavras-chave: Epidermal growth factor; Epidermal growth factor receptor; Hemolytic toxins; Immunotoxins; Monoclonal antibodies.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200008

Contributions by researchers of "Ciencia de Frontera" of the Chilean Academy of Sciences
Provedor de dados: 48
Autores: GONZALEZ-BILLAUL,CHRISTIAN; INESTROSA,NIBALDO C; SANTOS,MANUEL J.
Tipo: Journal article
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100001

The CK1 gene family: expression patterning in zebrafish development
Provedor de dados: 48
Autores: ALBORNOZ,AMELINA; YÁÑEZ,JOSÉ M; FOERSTER,CLAUDIA; AGUIRRE,CELESTE; PEREIRO,LUISA; BURZIO,VERÓNICA; MORAGA,MAURICIO; REYES,ARIEL E; ANTONELLI,MARCELO.
Protein kinase CK1 is a ser/thr protein kinase family which has been identified in the cytosol cell fraction, associated with membranes as well as in the nucleus. Several isoforms of this gene family have been described in various organisms: CK1á, CK1ß, CK1&amp;#948;, CK1å and CK1&amp;#947;. Over the last decade, several members of this family have been involved in development processes related to wnt and sonic hedgehog signalling pathways. However, there is no detailed temporal information on the CK1 family in embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe for the first time the cloning and detailed expression pattern of five CK1 zebrafish genes. Sequence analysis...
Tipo: Journal article
Palavras-chave: CK1; Casein Kinase 1; Expression pattern; Development; Zebrafish; Otic vesicles; Retina; Lens; Pectoral fin bud; Tegmentum; Myelencephalon; Telencephalon; Diencephalon; Mesencephalon; Rhombomeres.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200015

Secretory Patterns of Leptin and Luteinizing Hormone in Food-Restricted Young Female Sheep
Provedor de dados: 48
Autores: RECABARREN,SERGIO E; LOBOS,ALEJANDRO; TORRES,VERÓNICA; OYARZO,ROBERTO; SIR-PETERMANN,TERESA.
Leptin, the product of the ob gene, has been proposed as a metabolic signal that regulates the secretion of GnRH/LH. This may be critical during prepubertal development to synchronize information about energy stores and the secretion of GnRH/LH. This study aimed to assess the effect of food restriction on the episodic secretion of leptin and LH in young female sheep. Five 20-week-old prepubertal females were fed a low-level diet for 10 weeks to maintain the body weight. Control females of the same age received food ad libitum. Blood samples were collected at 10-min intervals for six hours at 20, 26, and 30 weeks of age, and plasma leptin, LH, insulin and cortisol concentrations were measured. In the control group, no changes were found in pulsatile LH...
Tipo: Journal article
Palavras-chave: Female sheep; Food restriction; Leptin; LH; Puberty.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300003

Long lasting structural changes in primary motor cortex after motor skill learning: a behavioural and stereological study
Provedor de dados: 48
Autores: MORALES,PAOLA.
Many motor skills, once acquired, are stored over a long time period, probably sustained by permanent neuronal changes. Thus, in this paper we have investigated with quantitative stereology the generation and persistence of neuronal density changes in primary motor cortex (MI) following motor skill learning (skilled reaching task). Rats were trained a lateralised reaching task during an "early" (22-31 days oíd) or "late" (362-371 days oíd) postnatal period. The trained and corresponding control rats were sacrificed at day 372, immediately after the behavioural testing. The "early" trained group preserved the learned skilled reaching task when tested at day 372, without requiring any additional training. The "late" trained group showed a similar capacity to...
Tipo: Journal article
Palavras-chave: Motor cortex; Plasticity; Persistence of structural changes; Aging; Motor skill learning (skilled reaching task); Stereology; Rat.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400005

Allometric scaling of biological rhythms in mammals
Provedor de dados: 48
Autores: Günther,Bruno; Morgado,Enrique.
A wide spectrum of cyclic functions in terrestrial mammals of different size, from the 3-gram shrew to the 3-ton elephant, yields an allometric exponent around 0.25, which is correlated _ as a kind of common denominator _ with the specific metabolic rate. Furthermore, the applicability of these empirical findings could be extrapolated to chronological events in the sub-cellular realm. On the other hand, the succession of growth periods (T98%) until sexual maturity is reached also follows the 1/4 power rule. By means of Verhulst's logistic equation, it has been possible to simulate three different biological conditions, which means that by modifying the numerical value of only one parameter, revertible physiological and pathological states can be obtained,...
Tipo: Journal article
Palavras-chave: Growth; Dimensional analysis; Specific metabolic rate; Logistic equation; Homeostasis.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200010

Time in physics and biology
Provedor de dados: 48
Autores: GÜNTHER,BRUNO; MORGADO,ENRIQUE.
In contrast with classical physics, particularly with Sir Isaac Newton, where time is a continuous function, generally valid, eternally and evenly flowing as an absolute time dimension, in the biological sciences, time is in essence of cyclical nature (physiological periodicities), where future passes to past through an infinitely thin boundary, the present. In addition, the duration of the present (DP) leads to the so-called 'granulation of time' in living beings, so that by the fusion of two successive pictures of the world, which are not entirely similar, they attain the perception of 'movement,' both in the real world as well as in the sham-movement in the mass media (TV).
Tipo: Journal article
Palavras-chave: Allometric equations; Biological time; Body mass; Physical time; Theory of biological similitude.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500005

Knockdown of ZFR suppresses cell proliferation and invasion of human pancreatic cancer
Provedor de dados: 48
Autores: Zhao,Xiaolan; Chen,Man; Tan,Jishan.
BACKGROUND: Zinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer. METHODS: Herein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluate ZFR gene expression in human pancreatic tumors. Then short hairpin RNA (shRNA) targeting ZFR was designed and delivered into PANC-1 pancreatic cancer cells to knock down ZFR expression. Cell viability, cell proliferation and cell cycle analysis after ZFR knockdown were determined by MTT, colony forming and FACS, respectively. In addition, cell migration and invasion were assessed using the Transwell system. RESULTS: The expression of ZFR was significantly...
Tipo: Journal article
Palavras-chave: Pancreatic cancer; RNA interference; Targeted therapy; Zinc finger RNA binding protein.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100026

Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer
Provedor de dados: 48
Autores: JIAN,HU; LI,SUN; LU-MING,WANG; SU-JUN,JIANG.
Several studies revealed a similar down-regulation of telomeric repeat binding factor 1 (TRF1) in tumors. We have previously reported the TRFl expression levels were down-regulation in non-small cell lung cancer (NSCLC). The regulation of TRFl localization is proposed to be important for the function and expression. The nuclear localization signal (NLS) and nuclear export signal (NES) are often important clues to localization of protein. The objective of the present study was to investigate the NLS and NES of TRFl in NSCLC patients. Thirty (30) patients with NSCLCs had undergone radical operations in The First Affiliated Hospital, College of Medicine, Zhejiang University. DNA sequences of NLSs and NESs were amplified by PCR. The PCR products were analyzed...
Tipo: Journal article
Palavras-chave: Non-small cell lung cancer; Telomeric repeat binding factor 1; Nuclear of localization signal; Nuclear of export signal; DNA sequencing.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200010

Characterization of mitochondrial iron uptake in HepG2 cells
Provedor de dados: 48
Autores: MAZARIEGOS,DORA INÉS; CABANTCHIK,Z. IOAV; CASSELS,BRUCE K; NÚÑEZ,MARCO TULIO.
There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron...
Tipo: Journal article
Palavras-chave: Mitochondria; Iron homeostasis; RPA; HepG2 cells.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024

Contribution of environmental pollutants to male infertily: A working model of germ cell apoptosis induced by plasticizers
Provedor de dados: 48
Autores: Lagos-Cabré,Raúl; Moreno,Ricardo D.
Bisphenol A [2,2-bis(4-hydroxyphenyl)propane] (BPA), 4-nonylphenol (NP) and di(2-ethylhexyl)phthalate (DEHP), and its metabolite mono-2-ethylhexyl phthalate (MEHP) are chemicals found in plastics, which act as endocrine disruptors (EDs) in animals, including human. EDs act like hormones in the endocrine system, and disrupt the physiologic function of endogenous hormones. Most people are exposed to different endocrine disruptors and concern has been raised about their true effect on reproductive organs. In the testis, they seem to preferentially attack developing testis during puberty rather than adult organs. However, the lack of information about the molecular mechanism, and the apparently controversial effect observed in different models has hampered the...
Tipo: Journal article
Palavras-chave: Testis; Spermatogenesis; Bisphenol A; Nonylphenol; ADAM17.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100001

Antioxidant Mechanisms of Polyphenolic Caffeic Acid Oligomers, Constituents of Salvia officinalis
Provedor de dados: 48
Autores: BORS,WOLF; CHRISTA,MICHEL; STETTMAIER,KURT; YINRONG,LU; L. YEAP,FOO.
Caffeic acid, rosmarinic acid and oligomers of caffeic acid with multiple catechol groups are all constituents of Salvia officinalis. Their antioxidant potential was investigated with regard to their radical scavenging activity and the stability and structure of the intermediate radicals. Pulse-radiolytic studies revealed very high rate constants with hydroxyl radicals. Evidence from kinetic modeling calculations suggested an unusual complex behavior due to the presence of both O4- and O3-semiquinones and formation and decay of a hydroxyl radical adduct at the vinyl side chain. The radical structures observed by EPR spectroscopy after autoxidation in slightly alkaline solutions were only partially identified due to their instability and generally...
Tipo: Journal article
Palavras-chave: Caffeic acid oligomer; Catechol semiquinone; Electron paramagnetic resonance; Hydroxyl radical; Kinetic modeling; Pulse radiolysis; Salvia officinalis.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200017

ABSTRACTS OF THE SPEAKERS' PRESENTATIONS
Provedor de dados: 48
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200019

Compositional studies and Biological activities of Perovskia abrotanoides Kar. oils
Provedor de dados: 48
Autores: Ashraf,Sadaf Naz; Zubair,Muhammad; Rizwan,Komal; Tareen,Rasool Bakhsh; Rasool,Nasir; Zia-Ul-Haq,Muhammad; Ercisli,Sezai.
BACKGROUND: Current study has been designed to evaluate the chemical composition of essential and fixed oils from stem and leaves of Perovskia abrotanoides and antioxidant and antimicrobial activities of these oils. RESULTS: GC-MS analysis of essential oil identified 19 compounds with (E)-9-dodecenal being the major component in stem and hexadecanoic acid in leaves. In contrast, GC-MS analysis of fixed oil showed 40 constituents with &amp;#945;-amyrin the major component in stem and &amp;#945;-copaene in leaves. The antioxidant activity showed the highest value of 76.7% in essential oil from leaves in comparison with fixed oil from stem (45.9%) through inhibition of peroxidation in linoleic acid system. The antimicrobial assay tested on different...
Tipo: Journal article
Palavras-chave: Perovskia abrotanoides; Essential oil; Fixed oil; Antioxidant capacity; Antimicrobial activity.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100012

Malnutrition affects the pressor response to microinjection of L-glutamate into the RVLM of awake rats
Provedor de dados: 48
Autores: Rodrigues,Fabiana Aparecida; Chianca-Jr,Deoclecio Alves; Gonçalves Fernandes,Luciano.
Post-weaning protein malnutrition is often related to the development of cardiovascular and metabolic diseases in humans, as well to changed content of neurotransmitters in the central nervous system under experimental conditions. The rostral ventrolateral medulla (RVLM) is a bulbar region that contains sympathetic premotor neurons; the excitatory amino acid L-glutamate seems to be the main neurotransmitter at this level. The aim of the present study was to evaluate the possible change in the L-glutamate sensitivity of the RVLM neurons of malnourished animals. Male Fischer rats were divided into two groups: control (n = 15) and malnourished (n = 19). Four days before the experiments, guide cannulas were implanted bilaterally in direction of the RVLM for...
Tipo: Journal article
Palavras-chave: Baroreflex; Blood pressure; L-glutamate receptors; Heart rate; Malnutrition; RVLM.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400002

Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
Provedor de dados: 48
Autores: Shen,Cong-Cong; Kang,Yu-Huan; Yu,Lin; Cui,Dan-Dan; He,Yi; Yang,Jin-Liang; Gou,Lan-Tu.
BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; TEX101; Seminoma; Testis; Western blotting.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100075

Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus
Provedor de dados: 48
Autores: Thao,Nguyen Phuong; Luyen,Bui Thi Thuy; Koo,Jung Eun; Kim,Sohyun; Koh,Young Sang; Cuong,Nguyen Xuan; Nam,Nguyen Hoai; Van Kiem,Phan; Kim,Young Ho; Van Minh,Chau.
BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-&amp;#945;) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ±0.01 to 26.19 ±0.64 &amp;#956;g/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus....
Tipo: Journal article
Palavras-chave: Protoreaster nodosus Starfish IL-12 p40 IL-6 TNF-&amp;#945; LPS-stimulated BMDCs Anti-inflammatory.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100012

Ca2+ entry, efflux and release in smooth muscle
Provedor de dados: 48
Autores: MATTHEW,A; SHMYGOL,A; WRAY,SUSAN.
Control of smooth muscle is vital for health. The major route to contraction is a rise in intracellular [Ca2+], determined by the entry and efflux of Ca2+ and release and re-uptake into the sarcoplasmic reticulum (SR). We review these processes in myometrium, to better understand excitation-contraction coupling and develop strategies for preventing problematic labours. The main mechanism of elevating [Ca2+] is voltage-gated L-type channels, due to pacemaker activity, which can be modulated by agonists. The rise of [Ca2+] produces Ca-calmodulin and activates MLCK. This phosphorylates myosin and force results. Without Ca2+ entry uterine contraction fails. The Na/Ca exchanger (NCX) and plasma membrane Ca-ATPase (PMCA) remove Ca2+, with contributions of 30%...
Tipo: Journal article
Palavras-chave: Uterus; SR; Signalling; Ca-ATPase.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400017

Organic acids, sugars, vitamin C content and some pomological characteristics of eleven hawthorn species (Crataegus spp.) from Turkey
Provedor de dados: 48
Autores: Gundogdu,Muttalip; Ozrenk,Koray; Ercisli,Sezai; Kan,Tuncay; Kodad,Ossama; Hegedus,Attila.
BACKGROUND: The Hawthorn (Crateagus sp.) mostly occurs around the temperate region of the world with a high number of species, producing a fruit with numerous beneficial effects for human health. The aim of the study was to determine organic acid and sugar contents in the fruit of a number of hawthorn species grown in Erzincan province of Turkey. RESULTS: Citric acid was the predominant organic acid in all hawthorn species and C. pseudoheterophylla had the highest citric acid content (23.688 g/100 g). There were not statistically significant differences among hawthorn species (except C. atrosanguinea Pojark) in terms of fumaric acid content. C. pontica C.Koch had a higher content of vitamin C (9.418 mg/100 g) compared to other species. Fructose was the...
Tipo: Journal article
Palavras-chave: Crataegus; Diversity; Fruit characteristics; Organic acids.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100021

Compensatory responses induced by oxidative stress in Alzheimer disease
Provedor de dados: 48
Autores: MOREIRA,PAULA I; ZHU,XIONGWEI; LIU,QUAN; HONDA,KAZUHIRO; SIEDLAK,SANDRA L; HARRIS,PEGGY L; SMITH,MARK A.; PERRY,GEORGE.
Oxidative stress occurs early in the progression of Alzheimer disease, significantly before the development of the pathologic hallmarks, neurofibrillary tangles and senile plaques. In the first stage of development of the disease, amyloid-&amp;#946; deposition and hyperphosphorylated tau function as compensatory responses and downstream adaptations to ensure that neuronal cells do not succumb to oxidative damage. These findings suggest that Alzheimer disease is associated with a novel balance in oxidant homeostasis.
Tipo: Journal article
Palavras-chave: Alzheimer disease; Antioxidant therapy; Mitochondria; Oxidative stress; Redox metals.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100002

In Vitro Genotoxic Effects of Four Helichrysum Species in Human Lymphocytes Cultures
Provedor de dados: 48
Autores: Erolu,Erhan H; Hamzaolu,Ergin; Aksoy,Ahmet; Budak,Ümit; Özkul,Yusuf.
Helichrysum sanguineum, Helichrysum pamphylicum, Helichrysum orientale, Helichrysum noeanum (Asteraceae) are medicinal plants. For centuries, they have been used as tea in Turkey because of their medicinal properties. So far no scientifc evidence has been found in a literature survey regarding the genotoxic effects of these plants. This work evaluated the genotoxic effects on human lymphocyte cultures induced by methanol extracts of these plants, assayed in different concentrations (0.01, 0.05, 0.1, 0.5 and 1 mg/mL). According to the results, Helichrysum noeanum, Helichrysum pamphylicum and Helichrysum sanguineum induced the formation of micronuclei and decreased the mitotic and replication indexes. Helichrysum orientale did not affect these parameters,...
Tipo: Journal article
Palavras-chave: Genotoxic; Helichrysum; Micronucleus; Mitotic index; Replication index.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200005

Age Dependence of the Levels of Plasma Norepinephrine, Aldosterone, Renin Activity and Urinary Vanillylmandelic Acid in Normal and Essential Hypertensives
Provedor de dados: 48
Autores: ABD-ALLAH,NADIA M; HASSAN,FAYEZA H; ESMAT,AMR Y; HAMMAD,SOMAYA A.
In the present study the upper reference limits (URLs) for resting plasma norepinephrine, epinephrine, serum aldosterone, plasma renin activity, aldosterone/ renin activity ratio, as well as urinary vanillylmandelic acid in healthy Egyptian normotensive subjects over a range of ages (5-60 yr) were established. There was a significant age effect on plasma norepinephrine, UVMA, serum aldosterone and PRA, whereas a single URL for plasma epinephrine level is satisfactory. In uncomplicated untreated essential hypertensive subjects (5-60 yr), the average prevalence of elevation in the plasma norepinephrine, epinephrine and urinary vanillylmandelic acid above their corresponding URLs was 85.10,62.15 and 83.20 %, respectively. This suggests that elevation in...
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100010

Arabidopsis thaliana: A model host plant to study plant-pathogen interaction using Chilean field isolates of Botrytis cinerea
Provedor de dados: 48
Autores: GONZÁLEZ,JUAN; REYES,FRANCISCA; SALAS,CARLOS; SANTIAG,MARGARITA; CODRIANSKY,YAEL; COLIHEUQUE,NELSON; SILVA,HERMAN.
One of the fungal pathogens that causes more agriculture damage is Botrytis cinerea. Botrytis is a constant threat to crops because the fungus infects a wide range of host species, both native and cultivated. Furthermore, Botrytis persists on plant debris in and on the soil. Some of the most serious diseases caused by Botrytis include gray mold on vegetables and fruits, such as grapes and strawberries. Botrytis also causes secondary soft rot of fruits and vegetables during storage, transit and at the market. In many plant-pathogen interactions, resistance often is associated with the deposition of callose, accumulation of autofluorescent compounds, the synthesis and accumulation of salicylic acid as well as pathogenesis-related proteins. Arabidopsis...
Tipo: Journal article
Palavras-chave: Arabidopsis thaliana; Botrytis cinerea; Plant-pathogen interaction; PR genes.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200004

Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
Provedor de dados: 48
Autores: González,Luis F.; Henríquez-Belmar,Francisca; Delgado-Acevedo,Claudia; Cisternas-Olmedo,Marisol; Arriagada,Gloria; Sotomayor-Zárate,Ramón; Murphy,Dennis L.; Moya,Pablo R.
Abstract  Background  Obsessive&amp;#8211;compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1&amp;#8211;3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus&amp;#8212;brain...
Tipo: Journal article
Palavras-chave: EAAT3 SLC1A1 Neuronal glutamate transporter Obsessive&amp;#8211; Compulsive disorder.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100225

UL146 variability among clinical isolates of Human Cytomegalovirus from Japan
Provedor de dados: 48
Autores: Aguayo,Francisco; Murayama,Tsugiya; Eizuru,Yoshito.
Human Cytomegalovirus (HCMV) is a herpesvirus associated with serious diseases in immunocompromised subjects. The region between ORF UL133 and UL151 from HCMV, named ULb' is frequently deleted in attenuated AD169 and in highly passaged laboratory strains. However, this region is conserved in low-passaged and more virulent HCMV, like the Toledo strain. The UL146 gene, which is located in the ULb' region, encodes a CXC-chemokine analogue. The diversity of UL146 gene was evaluated among fifty-six clinical isolates of HCMV from Japan. Results show that UL146 gene was successfully amplified by the polymerase chain reaction (PCR) in only 17/56 strains (30%), while the success rate for UL145/UL147 gene was 18/56 strains (32%). After DNA sequencing, the 35...
Tipo: Journal article
Palavras-chave: Cytomegalovirus; UL146; Sequence; V.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400013

Echinococcus granulosus protoscolex formation in natural infections
Provedor de dados: 48
Autores: GALINDO,MARIO; GONZALEZ,M JULIETA; GALANTI,NORBEL.
Echinococcus granulosus is a parasitic platyhelminth that is responsible for cystic hydatid disease. From the inner, germinal layer of hydatid cysts protoscoleces are generated, which are are the infective forms to the dog. Systematic studies on the cell biology of E. granulosus protoscolex formation in natural infections are scarce and incomplete. In the present report we describe seven steps in the development of protoscoleces. Cellular buds formed by a clustering of cells emerge from the germinal layer of hydatid cysts. The buds elongate and the cells at their bases seem to diminish in number. Very early on a furrow appears in the elongated buds, delimiting anterior (scolex) and caudal (body) regions. Hooks are the first fully-differentiated structures...
Tipo: Journal article
Palavras-chave: Protoscolex formation; Hydatid cyst.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300011

Modulation of cytosolic calcium signaling by protein kinase A-mediated phosphorylation of inositol 1,4,5-trisphosphate receptors
Provedor de dados: 48
Autores: STRAUB,STEPHEN V; WAGNER II,LARRY E; BRUCE,JASON I.E; YULE,DAVID I.
Calcium release via intracellular Ca2+ release channels is a central event underpinning the generation of numerous, often divergent physiological processes. In electrically non-excitable cells, this Ca2+ release is brought about primarily through activation of inositol 1,4,5-trisphosphate receptors and typically takes the form of calcium oscillations. It is widely believed that information is carried in the temporal and spatial characteristics of these signals. Furthermore, stimulation of individual cells with different agonists can generate Ca2+ oscillations with dramatically different spatial and temporal characteristics. Thus, mechanisms must exist for the acute regulation of Ca2+ release such that agonist-specific Ca2+ signals can be generated. One...
Tipo: Journal article
Palavras-chave: Inositol 1; 4; 5-trisphosphate receptor protein kinase A calcium signaling pancreatic acinar cells parotid acinar cells.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400013

FE DE ERRATA
Provedor de dados: 48
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500011

Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG
Provedor de dados: 48
Autores: SEIXAS,FABIANA K; BORSUK,SIBELE; FAGUNDES,MICHEL Q; HARTWIG,DAIANE D; SILVA,ÉVERTON F. DA; CERQUEIRA,GUSTAVO M; DELLAGOSTIN,ODIR A.
Mycobacterium bovis BCG has been proposed as an effective live vector for multivalent vaccines. The development of mycobacterial genetic systems to express foreign antigens and the adjuvanticity of BCG are the basis for the potential use of this attenuated mycobacterium as a recombinant vaccine vector. Stable plasmid vectors without antibiotic resistance markers are needed for heterologous antigen expression in BCG. Our group recently described the construction of a BCG expression system using auxotrophic complementation as a selectable marker. In this work, LipL32 and LigAni antigens of Leptospira interrogans were cloned and expressed in M. bovis BCG Pasteur and in the auxotrophic M. bovis BCG &amp;#916;leuD strains under the control of the M. leprae...
Tipo: Journal article
Palavras-chave: Recombinant BCG; Auxotrophic complementation; Foreign antigens.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100003

Ca-activated K channels: the ins and outs of calmodulin
Provedor de dados: 48
Autores: ORREGO,FERNANDO.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100006

Assessment of pharmacological activities of two medicinal plant of Bangladesh: Launaea sarmentosa and Aegialitis rotundifolia roxb in the management of pain, pyrexia and inflammation
Provedor de dados: 48
Autores: Raju,Golam Sarwar; Moghal,Md Mizanur Rahman; Hossain,Mohammad Salim; Hassan,Md Mahadi; Billah,Md Mustahsan; Ahamed,Sayed Koushik; Rana,SM Masud.
BACKGROUND: The current study aims at evaluating the analgesic, anti-pyretic and anti-inflammatory properties of methanolic extract of the stem, bark and leaves of Launaea sarmentosa and Aegialitis rotundifolia roxb. RESULTS: The AELS and AEAR extract presented a significant (***p &lt; 0.001) dose dependent increase in reaction time in writhing method and showed inhibition of 63.1% and 57.1% respectively at the doses of 400 mg/kg body weight while standard drug showed (P &lt; 0.001) inhibition of 69.23%. In tail immersion method, AELS and AEAR showed maximum time of tail retention at 30 min in hot water i.e. 6.93 sec and 6.54 sec respectively at highest doses of 400 mg/kg body weight than lower dose while standard pentazocine showed reaction time of 7.62...
Tipo: Journal article
Palavras-chave: Launaea sarmentosa; Aegialitis rotundifolia roxb; Analgesic; Antipyretic; Anti-inflammatory.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100049

Interplay between ER Ca2+ uptake and release fluxes in neurons and its impact on [Ca2+] dynamics
Provedor de dados: 48
Autores: FRIEL,DAVID.
In neurons, depolarizing stimuli open voltage-gated Ca2+ channels, leading to Ca2+ entry and a rise in the cytoplasmic free Ca2+ concentration ([Ca2+]i). While such [Ca2+]i elevations are initiated by Ca2+ entry, they are also influenced by Ca2+ transporting organelles such as mitochondria and the endoplasmic reticulum (ER). This review summarizes contributions from the ER to depolarization-evoked [Ca2+]i responses in sympathetic neurons. As in other neurons, ER Ca2+ uptake depends on SERCAs, while passive Ca2+ release depends on ryanodine receptors (RyRs). RyRs are Ca2+ permeable channels that open in response to increases in [Ca2+]i, thereby permitting [Ca2+]i elevations to trigger Ca2+ release through Ca2+-induced Ca2+ release (CICR). However, whether...
Tipo: Journal article
Palavras-chave: Dynamics; Sympathetic neurons; Endoplasmic reticulum; Ryanodine receptors.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400024

Analysis of rhythmic variance - ANORVA. A new simple method for detecting rhythms in biological time series
Provedor de dados: 48
Autores: Celec,Peter.
Cyclic variations of variables are ubiquitous in biomedical science. A number of methods for detecting rhythms have been developed, but they are often difficult to interpret. A simple procedure for detecting cyclic variations in biological time series and quantification of their probability is presented here. Analysis of rhythmic variance (ANORVA) is based on the premise that the variance in groups of data from rhythmic variables is low when a time distance of one period exists between the data entries. A detailed stepwise calculation is presented including data entry and preparation, variance calculating, and difference testing. An example for the application of the procedure is provided, and a real dataset of the number of papers published per day in...
Tipo: Journal article
Palavras-chave: Chronobiology; Time series; Method; ANORVA; Analysis of rhythmic variance; Circaseptan; Cycle; Rhyth.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500007

The 'whole-animal approach' as a heuristic principle in neuroscience research
Provedor de dados: 48
Autores: SERANI-MERLO,ALEJANDRO; PAZ,RODRIGO; CASTILLO,ANDRÉS.
Neuroscience embraces a heterogeneous group of disciplines. A conceptual framework that allows a better articulation of these different theoretical and experimental perspectives is needed. A `whole-animal approach' is proposed as a theoretical and hermeneutic tool. To illustrate the potential of this point of view, an overview of the research that has been performed in the extinction of fear-conditioned responses from Pavlov to the present is discussed. This is an example of how a whole-animal-based approach may help to organize and integrate basic and clinical neuroscience research. Our proposal is in agreement with recent statements calling for more integrative approaches in biological and neuropsychiatric research
Tipo: Journal article
Palavras-chave: Epistemology; Neurophilosophy; Fear conditioning; Extinction; Reductionism.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400008

Capital Humano en Ciencias
Provedor de dados: 48
Autores: DELGADO A,RICARDO.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100002

Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin
Provedor de dados: 48
Autores: OYARZÚN,MANUEL; DUSSAUBAT,NELSON; GONZÁLEZ,SERGIO.
To study the effect of ozone in a chronically damaged lung, we used a bleomycin (BLM) induced pulmonary fibrosis model. Both endotracheal instillation of BLM and O3 exposure both produce lung inflammation and fibrosis. Oxidative stress would be a common mechanism of damage for both BLM and O3. Our aim was to assess lung injury induced by 5 and 60 days of intermittent exposure to 0.25 ppm O3 in rats with bleomycin-induced pulmonary fibrosis. Thirty-day-old Sprague Dawley rats were endotracheally instilled with BLM (1 U/100 g body weight) and, 30 days later, exposed to 0.25 ppm O3 (0.25 ppm 4 h per day, 5 days a week). Histopatology controls were instilled with saline and breathing room air. Histopathological evaluation of lungs was done 5 and 60 days after...
Tipo: Journal article
Palavras-chave: Bleomycin; Histopathology; Lung damage; Ozone exposure.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400007

Evaluation of seasonal antioxidant activity and total phenolic compounds in stems and leaves of some almond (Prunus amygdalus L.) varieties
Provedor de dados: 48
Autores: Sivaci,Aysel; Duman,Sevcan.
BACKGROUND: This study aimed to determine the seasonal changes of total antioxidant activity and phenolic compounds in samples taken from leaves (April, July, October) and stems (April, July, October, January) of some almond (Prunus amygdalus L.) varieties (Nonpareil, Ferragnes and Texas). RESULTS: It was indicated that antioxidant activity and phenolic compounds in leaves and stems of Nonpareil, Ferragnes and Texas showed seasonal differences. Antioxidant activity IC50 of these varieties reached the highest value in April for leaves whereas in October for stems. The highest level of total phenolic compounds was in January for stems while in October for leaves. CONCLUSIONS: These results showed that total antioxidant activity and phenolics in leaves and...
Tipo: Journal article
Palavras-chave: Almond; Antioxidant activity; Phenolics; Prunus amygdalus; Seasonal changes.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100009

Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress
Provedor de dados: 48
Autores: LEITAO,LOUIS; MAORET,JEAN-JOSÉ; BIOLLEY,JEAN-PHILIPPE.
We quantified the ozone impact on levels of Zea mays L. cv. Chambord mRNAs encoding C4-phosphoenolpyruvate carboxylase (C4-PEPc), ribulose-l,5-bisphosphate carboxylase/oxygenase small and large subunits (Rubisco-SSU and Rubisco-LSU, respectively) and Rubisco activase (RCA) using real-time RT-PCR. Foliar pigment content, PEPc and Rubisco protein amounts were simultaneously determined. Two experiments were performed to study the ozone response of the 5th and the 10th leaf. For each experiment, three ozone concentrations were tested in open-top chambers: non-filtered air (NF, control) and non-filtered air containing 40 (+40) and 80 nL L-1 (+80) ozone. Regarding the 5th leaf, +40 atmosphere induced a loss in pigmentation, PEPc and Rubisco activase mRNAs....
Tipo: Journal article
Palavras-chave: Carboxylase; Maize; MRNA; Ozone; Rubisco activase.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200005

Phosphorylation of Ryanodine Receptors
Provedor de dados: 48
Autores: DANILA,CRISTINA I; HAMILTON,SUSAN L.
Both cardiac and skeletal muscle ryanodine receptors (RyRs) are parts of large complexes that include a number of kinases and phosphatases. These RyRs have several potential phosphorylation sites in their cytoplasmic domains, but the functional consequences of phosphorylation and the identity of the enzymes responsible have been subjects of considerable controversy. Hyperphosphorylation of Ser-2809 in RyR2 (cardiac isoform) and Ser-2843 in RyR1 (skeletal isoform) has been suggested to cause the dissociation of the FK506-binding protein (FKBP) from RyRs, producing "leaky channels," but some laboratories find no relationship between phosphorylation and FKBP binding. Also debated is the identity of the kinases that phosphorylate these serines: cAMP-dependent...
Tipo: Journal article
Palavras-chave: Ryanodine receptor; Phosphorylation site; Protein kinase; Ser-2809/Ser-2843; FK506-binding protein.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400005

Protective effects of lycopene on oxidative stress, proliferation and autophagy in iron supplementation rats
Provedor de dados: 48
Autores: Liu,Chongbin; Wang,Rui; Zhang,Bo; Hu,Chen; Zhang,Hong.
Lycopene is common in diet and known for its antioxidant activities. However, the impact of lycopene on iron metabolism is poorly investigated. In this study, we hypothesize that lycopene can prevent iron-mediated oxidative stress, proliferation and autophagy in liver and use a rat model of nutritional iron supplementation to confirm its intervention in these defence mechanisms. We found that iron supplementation induced cell proliferation predominantly in non parenchymal cells compared with hepatocytes, but not apoptosis. In addition, iron was accumulated within the hepatic lysosomes where it triggered autophagy as evidenced by the formation of autophagic vesicles detected by LC3-B staining. Iron supplementation also induced morphologic alterations of the...
Tipo: Journal article
Palavras-chave: Autophagy; Iron supplementation; Lycopene; Oxidative stress; Proliferation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200011

Premio Fred L. Soper 2001
Provedor de dados: 48
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100004

lnterleukin-8 gene polymorphism and susceptibility to gastric cancer in a brazilian population
Provedor de dados: 48
Autores: Felipe,Aledson V; Silva,Tiago D; Pimenta,Célia A; Kassab,Paulo; Forones,Nora M.
BACKGROUND: Studies have demonstrated that some polymorphisms in different interleukin genes may increase the risk of cancer. The aim of this study was to investigate the association between the IL-8 (rs4073) -251A/T gene polymorphism and the risk of gastric cancer (GC). PATIENTS AND METHODS: A case-control study was conducted on patients with noncardia gastric cancer. DNA was extracted from leukocytes and the IL-8 (rs4073) -251A/T polymorphism was analyzed by PCR-RFLP. Infection with Helicobacter pylori was investigated in the serum by ELISA. RESULTS: The sample consisted of 104 patients with GC and 196 controls. Cigarette smoking (P=0.007) and high fat intake (P=0.01) were more frequent in patients with GC. The proportion of patients infected with H....
Tipo: Journal article
Palavras-chave: IL-8; Polymorphism; Gastric cancer.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400007

Peroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean
Provedor de dados: 48
Autores: HERRIG,VANESSA; FERRARESE,MARIA DE LOURDES L.; SUZUKI,LETÍCIA S.; RODRIGUES,JOÃO DOMINGOS; FERRARESE-FILHO,OSVALDO.
The influence of the allelochemicals ferulic (FA) and vanillic (VA) acids on peroxidase (POD, EC 1.11.1.7) and phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activities and their relationships with phenolic acid (PhAs) contents and root growth of soybean (Glycine max (L.) Merr.) were examined. Three-day-old seedlings were cultivated in nutrient solution containing FA or VA (0.1 to 1 mM) for 48 h. Both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW) and increased PhAs contents. At 0.5 and 1 mM, FA increased soluble POD activity (18% and 47%, respectively) and cell wall (CW)-bound POD activity (61% and 34%), while VA increased soluble POD activity (33% and 17%) but did not affect CW-bound POD activity. At 1 mM, FA...
Tipo: Journal article
Palavras-chave: Roots; Phenolic acids; Phenylalanine ammonia-lyase; Peroxidase; Soybean.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100009

In vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from Spilanthes paniculata leaves
Provedor de dados: 48
Autores: Hossain,Mohammad Mobarak; Ahamed,Sayed Koushik; Dewan,Syed Masudur Rahman; Hassan,Md Mahadi; Istiaq,Arif; Islam,Mohammad Safiqul; Moghal,Md Mizanur Rahman.
BACKGROUND: The study was conducted to evaluate the in vitro antimicrobial activity, cytotoxic, and membrane stabilization activities, and in vivo antiemetic and antipyretic potentials of ethanolic extract, n-hexane and ethyl acetate soluble fractions of Spilanthes paniculata leaves for the first time widely used in the traditional treatments in Bangladesh. RESULTS: In antipyretic activity assay, a significant reduction (P &lt; 0.05) was observed in the temperature in the mice tested. At dose 400 mg/kg-body weight, the n-hexane soluble fraction showed the effect (36.7 ± 0.63°C ) as like as the standard (dose 150 mg/kg-body weight) after 5 h of administration. Extracts showed significant (P &lt; 0.001) potential when tested for the antiemetic activity...
Tipo: Journal article
Palavras-chave: Antiemetic; Anti-inflammatory; Antimicrobial activity; Brine shrimp lethality bioassay; Cytotoxic; Membrane stabilization; Spilanthes paniculata.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100045

Membrane effects of Cocoa Procyanidins in Liposomes and Jurkat T Cells
Provedor de dados: 48
Autores: VERSTRAETEN,SANDRA V.; OTEIZA,PATRICIA I.; FRAGA,CÉSAR G.
We investigated the effects of the interaction between flavanols and related procyanidins (dimer to hexamer) with both cell and synthetic membranes, on bilayer fluidity and susceptibility to oxidation. Cocoa derived dimers (0.05 to 1 µg/ml) protected Jurkat T cells from AMVN-mediated oxidation and increased plasma membrane fluidity. These effects occurred in a concentration- and chain length-dependent manner. In liposomes, procyanidins prevented the Fe2+-induced permeabilization of the membrane. Together, these results support the hypothesis that procyanidins could interact with the polar headgroup of lipids, increasing membrane fluidity and also, preventing the access of molecules that could affect membrane integrity
Tipo: Journal article
Palavras-chave: Epicatechin; Free radicals; Lipid oxidation; Membrane fluidity; Membrane permeability; Procyanidins.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200016

Classification methods for ongoing EEG and MEG signals
Provedor de dados: 48
Autores: BESSERVE,MICHEL; JERBI,KARIM; LAURENT,FRANCOIS; BAILLET,SYLVAIN; MARTINERIE,JACQUES; GARNERO,LINE.
Classification algorithms help predict the qualitative properties of a subject's mental state by extracting useful information from the highly multivariate non-invasive recordings of his brain activity. In particular, applying them to Magneto-encephalography (MEG) and electro-encephalography (EEG) is a challenging and promising task with prominent practical applications to e.g. Brain Computer Interface (BCI). In this paper, we first review the principles of the major classification techniques and discuss their application to MEG and EEG data classification. Next, we investigate the behavior of classification methods using real data recorded during a MEG visuomotor experiment. In particular, we study the influence of the classification algorithm, of the...
Tipo: Journal article
Palavras-chave: Brain computer interface; Electroencephalography; Magnetoencephalography; Visuomotor control; Support Vector Machine.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500005

Association of TNF promoter polymorphisms with type 1 diabetes in the South Croatian population
Provedor de dados: 48
Autores: BORASKA,VESNA; ŠKRABI,VESELIN; IKEŠ ULI,VEDRANA; BEI,KRISTIJAN; KAPITANOVI,SANJA; ZEMUNIK,TATIJANA.
Type 1 diabetes mellitus (TIDM) is an autoimmune disease characterized by the destruction of pancreatic p cells. Tumor necrosis factor (TNF) is a pleotropic cytokine with potent immunomodulatory and inflammatory activity. Association studies of TNF polymorphisms and type 1 diabetes (TIDM) frequently demonstrated TNF involvement with TIDM. Although TNF may play an important role in the pathogenesis of TIDM, the genetic association of TNF región with the disease has not been conclusive because of the strong linkage disequilibrium with HLA genes. In this study, we examined two TNF promoter variants (rs 1800629 at position -308, and rs361525 at position -238) for TIDM association in 233 patients and 144 controls from the population of South Croatia. A higher...
Tipo: Journal article
Palavras-chave: Type 1 diabetes; Tumor necrosis factor gene (TNF); Polymorphism; Genetic epidemiology; Croatia.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200005

Knockdown of interleukin-10 induces the redistribution of sigma1-receptor and increases the glutamate-dependent NADPH-oxidase activity in mouse brain neurons
Provedor de dados: 48
Autores: Koriauli,S.; Natsvlishvili,N.; Barbakadze,T.; Mikeladze,D.
BACKGROUND: In the central nervous system, interleukin-10 (IL-10) provides trophic and survival effects directly on neurons, modulates neurite plasticity, and has a pivotal importance in the neuronal regeneration in neurodegenerative and neuroinflammatory conditions. This cytokine is primarily produced by glial cells and has beneficial effects on the neuronal viability. However, the mechanisms of IL-10-elicited neuroprotection are not clear RESULTS: Membrane preparations, isolated from wild-type (Wt) and IL-10 knockout (KO) mice brain were used in this study. It has been shown that compared to wild-type mice, in IL-10 KO mice brain, the amount of immunoglobulin binding protein (BiP) is greatly increased, whereas the content of sigma receptor-1 (SigR1) is...
Tipo: Journal article
Palavras-chave: Interleukin-10; NMDA-receptor; NR2B subunit; NADPH-oxidase; Rac1; BiP; SigR1; ER stress; Neurodegeneration; Neuroinflammation.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100055

Biological applications of honeys produced by Apis mellifera
Provedor de dados: 48
Autores: Montenegro,Gloria; Mejías,Enrique.
Honey is a natural product with many attributes that are useful for humans. The consumption of honey is increasing because of its beneficial biological properties, including antioxidant and antibacterial activities. Even though honey is produced worldwide, many variants of this product have not been studied and their biological potential for alternative uses has not been evaluated. Because of its varied endemic native flora, Chile produces several types of honey. It is likely that these apian products have important biological properties inherited from specific floral sources, but it is absolutely necessary to conduct further chemical analyses to identify and characterize these biological attributes. Sadly, the potential antimicrobial and antitumor...
Tipo: Journal article
Palavras-chave: Honeys; Antioxidants; Natural Antibiotics; Environmental Bioindicator.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400005

In vitro response of date palm (Phoenix dactylifera L.) to K/Na ratio under saline conditions
Provedor de dados: 48
Autores: Alkhateeb,Suliman A; Alkhateeb,Abdullatif A; Solliman,Mohei EL-Din.
BACKGROUND: Salinity is a serious factor limiting the productivity of agricultural plants. One of the potential problems for plants growing under saline conditions is the inability to up take enough K+. The addition of K+ may considerably improve the salt tolerance of plants grown under salinity. It is assumed that increasing the K+ supply at the root zone can ameliorate the reduction in growth imposed by high salinity. The present study aims to determine whether an increase in the K/Na ratio in the external media would enhance the growth of date palm seedlings under in vitro saline conditions METHODS: Date palm plants were grown at four concentrations of Na + K/Cl (mol/m³) with three different K/Na ratios. The 12 salt treatments were added to modified MS...
Tipo: Journal article
Palavras-chave: Date palm; In vitro; Ion relations; K/Na ratio; Salinity.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100063

The balance between GMD and OFUT1 regulates Notch signaling pathway activity by modulating Notch stability
Provedor de dados: 48
Autores: Glavic,Alvaro; López-Varea,Ana; de Celis,José F.
The Notch signaling pathway plays an important role in development and physiology. In Drosophila, Notch is activated by its Delta or Serrate ligands, depending in part on the sugar modifications present in its extracellular domain. O-fucosyltransferase-1 (OFUT1) performs the first glycosylation step in this process, O-fucosylating various EGF repeats at the Notch extracellular domain. Besides its O-fucosyltransferase activity, OFUT1 also behaves as a chaperone during Notch synthesis and is able to down regulate Notch by enhancing its endocytosis and degradation. We have reevaluated the roles that O-fucosylation and the synthesis of GDP-fucose play in the regulation of Notch protein stability. Using mutants and the UAS/Gal4 system, we modified in developing...
Tipo: Journal article
Palavras-chave: Notch; GMD; O-fucosyltranserase; Drosophila.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100004

The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis
Provedor de dados: 48
Autores: Xu,Yuan-kun; Ke,Yan; Wang,Bin; Lin,Jian-hao.
BACKGROUND: Osteoarthritis (OA) is a common arthritic disease and multifactorial whole-joint disease. Interactions of chemokines and OA is inadequately documented RESULTS: In vivo and in vitro studies were conducted to investigate monocyte chemoattractant protein 1 (MCP-1) and receptor chemokine (C-C motif) receptor 2 (CCR2) in chondrocyte degradation and cartilage degeneration. Chondrocytes from 16 OA patients and 6 normal controls were involved in this study. After stimulation of MCP-1, the expression of MCP-1 and CCR2 increased significantly (P &lt; 0.001) and the expression of MMP-13 also increased (P &lt; 0.05). MCP-1 stimulation also induced (or enhanced) the apoptosis of OA chondrocytes (P &lt; 0.05). Additionally, the degradation of cartilage...
Tipo: Journal article
Palavras-chave: MCP-1; CCR2; Osteoarthritis; Chondrocyte; Rat; Mouse.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100064

Modulation of sarcoplasmic reticulum calcium release by calsequestrin in cardiac myocytes
Provedor de dados: 48
Autores: GYÖRKE,SANDOR; GYÖRKE,INNA; TERENTYEV,DMITRY; VIATCHENKO-KARPINSKI,SERGE; WILLIAMS,SIMON C.
Calsequestrin (CASQ2) is a high capacity Ca-binding protein expressed inside the sarcoplasmic reticulum (SR). Mutations in the cardiac calsequestrin gene (CASQ2) have been linked to arrhythmias and sudden death induced by exercise and emotional stress. We have studied the function of CASQ2 and the consequences of arrhythmogenic CASQ2 mutations on intracellular Ca signalling using a combination of approaches of reverse genetics and cellular physiology in adult cardiac myocytes. We have found that CASQ2 is an essential determinant of the ability of the SR to store and release Ca2+ in cardiac muscle. CASQ2 serves as a reservoir for Ca2+ that is readily accessible for Ca2+-induced Ca2+ release (CICR) and also as an active Ca2+ buffer that modulates the local...
Tipo: Journal article
Palavras-chave: Excitation-contraction coupling; Calcium-induced calcium release; Ryanodine receptor; Calsequestrin; Arrhythmia.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400014

Chlorpheniramine impairs spatial choice learning in telencephalon-ablated fish
Provedor de dados: 48
Autores: ROMAGUERA,FERNANDA; MATTIOLI,ROSANA.
This work investigated the effect of the Hj receptor blockade in the forebrain of ablated Carassius auratus in a simple stimulus-response learning task using a T-maze test with positive reinforcement. The goldfish were submitted to surgery for removal of both telencephalic lobes five days before beginning the experiment. A T-shaped glass aquarium was employed, with two feeders located at the extremities of the long arm. One of the two feeders was blocked. The experimental triáis were performed in nine consecutive days. Each fish was individually placed in the short arm and confined there for thirty seconds, then it was allowed to swim through the aquarium to search for food for ten minutes (máximum period). Time to find food was analysed in seconds....
Tipo: Journal article
Palavras-chave: Histamine; Learning; Teleost fish; Telencephalon.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300010

Altered dopamine levels induced by the parasite Profilicollis antarcticus on its intermediate host, the crab Hemigrapsus crenulatus
Provedor de dados: 48
Autores: ROJAS,JOSÉ MIGUEL; OJEDA,F. PATRICIO.
A serotonergic pathway is apparently involved in parasite-host interactions. Previous studies conducted in our laboratory showed increased rates in oxygen consumption and alterations in body posture in the crab Hemigrapsus crenulatus parasitized by the acanthocephalan, Profilicollis antarcticus. Such changes may be related to the functions described for biogenic amines in crustaceans. During the infective stage the acanthocephalans live freely in the hemocelomic cavity, suggesting that the possible alteration induced by biogenic amines may be related to their neurohormonal function in crustaceans. To test whether the presence of P. antarcticus produced neurohormonal changes in its intermediate host, H. crenulatus, we analyzed serotonin and dopamine levels...
Tipo: Journal article
Palavras-chave: Acanthocephalans; Biogenic amines; HPLC; Parasite-host interaction; Parasite transmission.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200015

mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
Provedor de dados: 48
Autores: Panariti,Alice; Miserocchi,Giuseppe; Rivolta,Ilaria.
This study analyzed the time dependence decay of the mRNA of selected genes important for the hypoxia response. The genes chosen were the two isoforms of hypoxia-inducible factors, the three isoforms of the prolyl hydroxylase domain protein, the vascular endothelial growth factor and endothelial nitric oxide synthase. mRNA and proteins were extracted from lungs obtained from control, hypoxic and 15 minutes normoxic recovered rats and analyzed by Real-time RT-PCR or by the Western Blot technique. Results indicated that in normoxia isoform 2á was the more represented hypoxia-inducible factor mRNA, and among the prolyl hydroxylase domain transcripts, isoform 3 was the least abundant. Moreover, in chronic hypoxia only hypoxia-inducible factor 1&amp;#945; and...
Tipo: Journal article
Palavras-chave: Chronic hypoxia; Hypoxia-inducible Factor; Prolyl Hydroxylase Domain proteins; Lung; RT-PCR.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200008

COMUNICACIONES ORALES
Provedor de dados: 48
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000300005

Alterations in core histone variant ratios during maize root differentiation, callus formation and in response to plant hormone treatment
Provedor de dados: 48
Autores: ALATZAS,ANASTASIOS; FOUNDOULI,ATHINA.
Although several histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study we evaluated the ratio of each histone variant in each of the four core histone classes in the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them, in order to define possible alterations either during plant cell differentiation or dedifferentiation. We also evaluated core histone variant ratios in the developmental zones of roots treated with auxin and gibberellin in order to examine the effects of exogenously applied plant hormones to histone...
Tipo: Journal article
Palavras-chave: Callus culture; Histone variant; Plant cell differentiation; Plant hormone; Zea mays.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400006

Antitumor function and mechanism of phycoerythrin from Porphyra haitanensis
Provedor de dados: 48
Autores: Pan,Qunwen; Chen,Meizhen; Li,Juan; Wu,Yan; Zhen,Chao; Liang,Bin.
The anti-tumor effect of R-Phycoerythrin (R-PE) from Porphyra haitanensis was studied using cell line HeLa as an in vitro model and Sarcoma-180 (S180) tumor-bearing mice as an in vivo model. The results showed that the combination treatment of R-PE and photodynamic therapy PDT) significantly inhibited the growth of HeLa cells up to 81.5%, with a fair dose-effect relationship, but did not inhibit endothelial cells. The annexin v-fitc/PI fluorescence staining experiments demonstrated that at doses between 0~60µg/mL, apoptosis cells and later stage apoptosis cells or necrosis cells increased significantly as the R-PE dosage increased. DNA electrophoresis showed that after R-PE+PDT treatment of HeLa cells for 24 hours, a light "smear" band between 100~400bp...
Tipo: Journal article
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100013

Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors
Provedor de dados: 48
Autores: FARRELL,EMILY F; ANTARAMIAN,ANAID; BENKUSKY,NANCY; ZHU,XINSHENG; RUEDA,ANGÉLICA; GÓMEZ,ANA M; VALDIVIA,HÉCTOR H.
Activation of Ca2+ release channels/ryanodine receptors (RyR) by the inward Ca2+ current (I Ca) gives rise to Ca2+-induced Ca2+ release (CICR), the amplifying Ca2+ signaling mechanism that triggers contraction of the heart. CICR, in theory, is a high-gain, self-regenerating process, but an unidentified mechanism stabilizes it in vivo. Sorcin, a 21.6 kDa Ca2+-binding protein, binds to cardiac RyRs with high affinity and completely inhibits channel activity. Sorcin significantly inhibits both the spontaneous activity of RyRs in quiescent cells (visualized as Ca2+ sparks) and the I Ca-triggered activity of RyRs that gives rise to [Ca2+]i transients. Since sorcin decreases the amplitude of the [Ca2+]i transient without affecting the amplitude of I Ca, the...
Tipo: Journal article
Palavras-chave: Sorcin; Ryanodine receptors; CICR; Dihydropyridine receptor; Sarcoplasmic reticulum.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400015

Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
Provedor de dados: 48
Autores: MISHRA,PANKAJ K; MISHRA,SMITA; BISHT,SHEKHAR C; SELVAKUMAR,G; KUNDU,S; BISHT,J. K; GUPTA,HARI SHANKAR.
A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 &amp;#956;m in size). It grew at temperatures ranging from 4 to 30°C, with a growth optimum at 28°C. It exhibited tolerance to a wide pH range (5-10; optimum 8.0) and salt concentrations up to 6% (wt/vol). Although it was sensitive to Rifampicin (R 20 &amp;#956;g mi-1), Gentamicin (G 3 &amp;#956;g mi-1), and Streptomycin (S 5 &amp;#956;g mi-1), it showed resistance to higher concentrations of Ampicillin (A 500 &amp;#956;g mi-1), Penicillin (P 300 &amp;#956;g mi-1), Polymixin B...
Tipo: Journal article
Palavras-chave: Cold tolerant; Pseudomonas lurida; 16S rRNA gene; Nutrient uptake; Plant growth promotion.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300005

Andrés Weintraub: Premio Nacional de Ciencias Aplicadas 2000
Provedor de dados: 48
Autores: MEYER Z.,LEONARDO.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300002

Disease association and inter-connectivity analysis of human brain specific co-expressed functional modules
Provedor de dados: 48
Autores: Oh,Kimin; Hwang,Taeho; Cha,Kihoon; Yi,Gwan-Su.
BACKGROUND: In the recent studies, it is suggested that the analysis of transcriptomic change of functional modules instead of individual genes would be more effective for system-wide identification of cellular functions. This could also provide a new possibility for the better understanding of difference between human and chimpanzee RESULTS: In this study, we analyzed to find molecular characteristics of human brain functions from the difference of transcriptome between human and chimpanzee's brain using the functional module-centric co-expression analysis. We performed analysis of brain disease association and systems-level connectivity of species-specific co-expressed functional modules CONCLUSIONS: Throughout the analyses, we found human-specific...
Tipo: Journal article
Palavras-chave: Co-expressed functional module; Disease association; Inter-connectivity analysis.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100067

SIMPOSIOS
Provedor de dados: 48
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000300003

Characterization and mapping of an informational suppressor in Aspergillus nidulans
Provedor de dados: 48
Autores: GRACITE DE MELO FRANZONI,MELISSA; ALVES DE CASTRO-PRADO,MARIALBA AVEZUM.
The present work was undertaken to characterize a suppressor gene present in a mutant strain of A. nidulans obtained with NTG (N-Methyl-N'-Nitro-N-Nitrosoguanidine). Analyses of this mutant have shown that this suppressor, designated suO1, induces phenotypic co-reversion of several auxotrophic mutations and makes the strain sensitive to aminoglycoside antibiotics and lower temperatures. suO1 has shown to be on linkage group VIII. The vegetative growth of the mutant strain is very unstable because the suppressor gene induces the production of prototrophic mitotic sectors. The strains bearing the suO1 gene produce cleistothecia containing a reduced number of viable ascospores during the sexual cycle. The segregation of the genetic markers has also been...
Tipo: Journal article
Palavras-chave: Aspergillus nidulans; Barren cleistothecia; Informational suppressor; Paromomycin.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100008

BJ-1108, a 6-Amino-2,4,5-trimethylpyridin-3-ol analogue, regulates differentiation of Th1 and Th17 cells to ameliorate experimental autoimmune encephalomyelitis
Provedor de dados: 48
Autores: Kang,Youra; Timilshina,Maheshwor; Nam,Tae-gyu; Jeong,Byeong-Seon; Chang,Jae-Hoon.
Abstract  Background  CD4+ T cells play an important role in the initiation of an immune response by providing help to other cells. Among the helper T subsets, interferon-&amp;#947; (IFN-&amp;#947;)-secreting T helper 1 (Th1) and IL-17-secreting T helper 17 (Th17) cells are indispensable for clearance of intracellular as well as extracellular pathogens. However, Th1 and Th17 cells are also associated with pathogenesis and contribute to the progression of multiple inflammatory conditions and autoimmune diseases.  Results  In the current study, we found that BJ-1108, a 6-aminopyridin-3-ol analogue, significantly inhibited Th1 and Th17 differentiation in vitro in a concentration-dependent manner, with no effect on proliferation or apoptosis of activated T...
Tipo: Journal article
Palavras-chave: BJ-1108; Th1/Th17 cell; Differentiation; EAE.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100202

LA INVENCION DE LA CELULA
Provedor de dados: 48
Autores: VIAL CORREA,JUAN DE DIOS.
Tipo: Journal article
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400004

Disruption of reproductive development in male rat offspring following gestational and lactational exposure to di-(2-ethylhexyl) phthalate and genistein
Provedor de dados: 48
Autores: Zhang,Lian-Dong; Deng,Qian; Wang,Zi-Ming; Gao,Ming; Wang,Lei; Chong,Tie; Li,He-Cheng.
Studies of developmental effects of mixtures of endocrine disrupters on the male reproductive system are of great concern. In this study, the reproductive effects of the co-administration of di-2-(ethylhexyl) phthalate (DEHP) and genistein (GEN) during pregnancy and lactation were studied in male rat offspring. Pregnant Sprague-Dawley rats were gavaged from gestation day 3 to postnatal day 21 with vehicle control, DEHP 250 mg/kg body weight (bwyday, GEN 50 mg/kg bwday, GEN 400 mg/kg bwday, and two combinations of the two compounds (DEHP 250 mg/kg bwday + GEN 50 mg/kg bwday, DEHP 250 mg/kg bwday + GEN 400 mg/kg bwday). The outcomes studied were general morphometry (weight, AGD), testicular histology, testosterone levels, and expression at the mRNA level of...
Tipo: Journal article
Palavras-chave: Di-2-(ethylhexyl) phthalate; Genistein; Pregnancy; Lactation; Reproductive system.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200004

Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii
Provedor de dados: 48
Autores: Norasfaliza Rahmad,Jameel R Al-Obaidi; Rashid,Noraswati Mohd Nor; Zean,Ng Boon; Yusoff,Mohd Hafis Yuswan Mohd; Shaharuddin,Nur Syahidah; Jamiland,Nor Azreen Mohd; Saleh,Norihan Mohd.
BACKGROUND: Termitomyces heimii is a basidiomycete fungus that has a symbiotic relationship with termites, and it is an edible mushroom with a unique flavour and texture. T. heimii is also one of the most difficult mushrooms to cultivate throughout the world. Little is known about the growth and development of these mushrooms, and the available information is insufficient or poor. The purpose of this study was to provide a base of knowledge regarding the biological processes involved in the development of T. heimii. The proteomic method of 2 dimensional difference gel electrophoresis 2D-DIGE was used to determine and examine the protein profiles of each developmental stage (mycelium, primordium and fruiting body). Total proteins were extracted by...
Tipo: Journal article
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100030

Pablo Valenzuela, Premio Nacional de Ciencias Aplicadas y Tecnológicas 2002: Protagonista y Testigo de una Revolución: La Industria Genética
Provedor de dados: 48
Autores: AYERDI DELMON,CATALINA.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300003

The monthly rhythm of incidence and age at menarche: thirty five years of research: The circa-vacation-study expectancy rhythm of incidence and age at menarche
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
The hypothesis that the vacation-study-expectancy scholar regime produces most of the monthly rhythm of the age at menarche (AaM) was tested. Studies on monthly menarche incidence (MI) refuted climatic factors as a main factor in this rhythm, and indicated that the main factor of this rhythm is the succession of expectancies of study (Stu-months) or vacation (Vac-months) months within a year. Thus the hypothesis of seasonal circa-annual rhythm should be modified to the circa-[vacation (fiesta)]-[study (non-fiesta)]-expectancies rhythm for the MI and age at menarche annual rhythms. In several countries Vac-months had higher MI than Stu-months. The high MI of Vac-months was followed by a large decrease when girls started their studies and a MI increase...
Tipo: Journal article
Palavras-chave: Age at menarche; Annual rhythm; Circa-vacation-study-expectancy rhythm; Fiesta expectancy; Menarche incidence.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100003

In vitro antioxidant capacity and free radical scavenging evaluation of active metabolite constituents of Newbouldia laevis ethanolic leaf extract
Provedor de dados: 48
Autores: Habu,Josiah Bitrus; Ibeh,Bartholomew Okechukwu.
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Tipo: Journal article
Palavras-chave: Newbouldia laevis; Phytochemicals; Ethanolic extraction; Antioxidants; Free radical scavengers; Bioactive constituents.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100016

A snapshot of cancer in Chile: analytical frameworks for developing a cancer policy
Provedor de dados: 48
Autores: la Jara,Jorge Jimenez de; Bastias,Gabriel; Ferreccio,Catterina; Moscoso,Cristian; Sagues,Sofia; Cid,Camilo; Bronstein,Eduardo; Herrera,Cristian; Nervi,Bruno; Corvalan,Alejandro; Velasquez,Ethel V; Gonzalez,Pamela; Castellon,Enrique; Bustamante,Eva; Oñate,Sergio; McNerney,Eileen; Sullivan,Richard; Owen,Gareth I.
INTRODUCTION: The South American country Chile now boasts a life expectancy of over 80 years. As a consequence, Chile now faces the increasing social and economic burden of cancer and must implement political policy to deliver equitable cancer care. Hindering the development of a national cancer policy is the lack of comprehensive analysis of cancer infrastructure and economic impact. OBJECTIVES: Evaluate existing cancer policy, the extent of national investigation and the socio-economic impact of cancer to deliver guidelines for the framing of an equitable national cancer policy. METHODS: Burden, research and care-policy systems were assessed by triangulating objective system metrics -epidemiological, economic, etc. - with political and policy analysis....
Tipo: Journal article
Palavras-chave: Chile; Cancer policy; Investigation; Research and development; Statistics; Gallbladder cancer; Stomach cancer; Developing country.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100010

Molecular interplay between ion channels and the regulation of apoptosis
Provedor de dados: 48
Autores: RAZIK,MONA A; CIDLOWSKI,JOHN A.
Apoptosis is the programmed and deliberate destruction of specific cells. This process occurs during normal development and maintains cellular homeostasis. Disruption or malfunction of apoptosis is implicated in diseases like cancer, AIDS as well as neurodegenerative disorders. The movement of monovalent ions appears to set the stage for the induction of the self-destruction machinery by creating an intracellular environment that favors activation and coordinated execution of the apoptotic program. Understanding the components and steps involved in this intricate process can further our insight to diseases and reveal new approaches for therapeutic treatment
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200011

Effects of abiotic stress on biomass and anthocyanin production in cell cultures of Melastoma malabathricum
Provedor de dados: 48
Autores: CHAN,L. K; KOAY,S. S; BOEY,P. L; BHATT,A.
Plant cell cultures could be used as an important tool for biochemical production, ranging from natural coloring (pigments) to pharmaceutical products. Anthocyanins are becoming a very important alternative to synthetic dyes because of increased public concern over the safety of artificial food coloring agents. Several factors are responsible for the production of anthocynin in cell cultures. In the present study, we investigate the effects of different environmental factors, such as light intensity, irradiance (continuous irradiance or continuous darkness), temperature and medium pH on cell biomass yield and anthocyanin production in cultures of Melastoma malabathricum. Moderate light intensity (301 - 600 lux) induced higher accumulation of anthocyanins...
Tipo: Journal article
Palavras-chave: Irradiance; Medium pH; Photoperiod; Temperature; Melastoma malabathricum.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100014

Gametogenesis and nucleotypic effects in the tetraploid red vizcacha rat, Tympanoctomys barrerae (Rodentia, Octodontidae)
Provedor de dados: 48
Autores: GALLARDO,MILTON H; GARRIDO,ORLANDO; BAHAMONDE,RAÚL; GONZÁLEZ,MARCELO.
Nucleotypic effects link DNA content with nuclear size and cell dimensions of reproductive cells in polyploid organisms. We studied the gametogenesis of the allotetraploid rodent Tympanoctomys barrerae, aiming to determine these effects in reproductive cells. The species' cofamily members, Octodon degus and Spalacopus cyanus were used as control. Spermatogenesis and oogenesis in T. barrerae follows the pattern of differentiation and sequence of events of the control species, but varied nucleotypic effects were observed. Exceedingly large, spatulated spermatozoa with a submedially attached flagellum are characteristic of male T. barrerae. The diameter of the nuclei of primordial and growing follicles as well as those of the Graaff follicles, of the...
Tipo: Journal article
Palavras-chave: Nucleotypic effects; Oogenesis; Spermatogenesis; Octodontidae; Tympanoctomys barrerae; Tetraploidy.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500006

Acclimatization to chronic intermittent hypoxia in mine workers: a challenge to mountain medicine in Chile
Provedor de dados: 48
Autores: Farias,Jorge G; Jimenez,Daniel; Osorio,Jorge; Zepeda,Andrea B; Figueroa,Carolina A; Pulgar,Victor M.
In the past two decades, Chile has developed intense mining activity in the Andes mountain range, whose altitude is over 4,000 meters above sea level. It is estimated that a workforce population of over 55,000 is exposed to high altitude hypobaric hypoxia. The miners work under shift systems which vary from 4 to 20 days at the worksite followed by rest days at sea level, in a cycle repeated for several years. This Chronic Intermittent Hypoxia (CIH) constitutes an unusual condition for workers involving a series of changes at the physiological, cellular and molecular levels attempting to compensate for the decrease in the environmental partial pressure of oxygen (PO2). The mine worker must become acclimatized to CIH, and consequently undergoes an acute...
Tipo: Journal article
Palavras-chave: High Altitude; Mine Workers; Intermittent Hypoxia; Acclimatization; Chilean Model.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100009

Biological Research Factor de Impacto 1,154
Provedor de dados: 48
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300006

Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
Provedor de dados: 48
Autores: ROJAS-DOTOR,SARA; PÉREZ-RAMOS,JULIA; GIMÉNEZ-SCHERER,JUAN ANTONIO; BLANCO-FAVELA,FRANCISCO; RICO-ROSILLO,GUADALUPE.
Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-lß, IFN-&amp;#947;, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1...
Tipo: Journal article
Palavras-chave: Amebic hepatic abscess (AHA) CC chemokine receptors (CCR4; CCR5) monocyte locomotion inhibitory factor (MLIF) Entamoeba histolytica (E. histolytica) 1-phorbol-12 myristate-13 acetate (PMA) interleukin (IL) T helper 1 lymphocyte (Th1) T helper 2 lymphocyte Th2).
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003

Genetic polymorphism at position -308 in the promoter region of the tumor necrosis factor (TNF): Implications of its allelic distribution on susceptibility or resistance to diseases in the Chilean population
Provedor de dados: 48
Autores: CUENCA,JIMENA; PÉREZ,CLAUDIO A.; AGUIRRE,ADAM J.; SCHIATTINO,IRENE; AGUILLÓN,J. CARLOS.
Several single-nucleotide polymorphisms have been identified in the human TNF gene promoter. The polymorphism at position _308 (TNF-308), which involves substituting G for A and designing the TNF2 allele, leads to a higher rate of TNF gene transcription than the wild-type TNF1 allele in in vitro expression studies. It has also been linked to increased susceptibility to a variety of illnesses. Using PCR-RFLP analysis we detected significant differences in the TNF-308 genotypes of Chilean and other populations. We conclude that there is a gradient in the distribution of the TNF2 allele according to ethnicity; we have also hypothesized that populations bearing a higher proportion of the TNF2 allele may have an increased predisposition toward or incidence of...
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300011

Cytotoxic activity of some marine brown algae against cancer cell lines
Provedor de dados: 48
Autores: KHANAVI,MAHNAZ; NABAVI,MARYAM; SADATI,NARGESS; SHAMS ARDEKANI,MOHAMMADREZA; SOHRABIPOUR,JELVE; NABAVI,SEYED MOHAMMAD B; GHAELI,PADIDEH; OSTAD,SEYED NASSER.
The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70%) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf against in different cell lines including HT-29, Caco-2, T47D, MDA-MB468 and NIH 3T3 cell lines by MTT and AnnexinV-PI assay. The hexan fraction of S. swartzii and C. myrica showed selective cytotoxicity against proliferation of Caco-2 cells (IC50&lt;100 &amp;#956;g/ml) T47D cell line (IC50&lt;100 &amp;#956;g/ml), respectively. S. swartzii and C. myrica were also observed for increasing apoptosis in Caco-2 and T47D cells. Total extract and fractions of C....
Tipo: Journal article
Palavras-chave: Colpomenia sinuosa; Cystoseira myrica; Cytotoxic activity; MTT assay; Persian Gulf; Sargassum swartzii.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100005

Anticancer properties and enhancement of therapeutic potential of cisplatin by leaf extract of Zanthoxylum armatum DC
Provedor de dados: 48
Autores: Singh,Thangjam Davis; Meitei,Heikrujam Thoihen; Sharma,Adhikarimayum Lakhikumar; Robinson,Asem; Singh,Lisam Shanjukumar; Singh,Thiyam Ramsing.
BACKGROUND: Clinical use of chemotherapeutic drug, cisplatin is limited by its toxicity and drug resistance. Therefore, efforts continue for the discovery of novel combination therapies with cisplatin, to increase efficacy and reduce its toxicity. Here, we screened 16 medicinal plant extracts from Northeast part of India and found that leaf extract of Zanthoxylum armatum DC. (ZALE) induced cytotoxicity as well as an effect on the increasing of the efficiency of chemotherapeutic drugs (cisplatin, mitomycin C and camptothecin). This work shows detail molecular mechanism of anti-cancer activity of ZALE and its potential for combined treatment regimens to enhance the apoptotic response of chemotherapeutic drugs. RESULTS: ZALE induced cytotoxicity, nuclear...
Tipo: Journal article
Palavras-chave: Zanthoxylum armatum; Apoptosis; ERK activation; Cisplatin sensitization; DNA damage.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100046

Differential expression of upstream stimulatory factor (USF) 2 variants in eutopic endometria from women with endometriosis: estradiol regulation
Provedor de dados: 48
Autores: Castro,Jazmin; Araya,Germán; Inostroza,Pamela; Hidalgo,Paulina; González-Ramos,Reinaldo; Sovino,Hugo; Angélica Boric,M.; Fuentes,Ariel; Cecilia Johnson,M.
BACKGROUND: Endometriosis, pro-inflammatory and invasive benign disease estrogen dependent, abnormally express in endometria the enzyme P450Arom, positively regulated by steroid factor-1 (SF-1). Our objective was to study the nuclear protein contents of upstream stimulating factor 2 (USF2a and USF2b), a positive regulator of SF-1, throughout the menstrual cycle in eutopic endometria from women with and without (control) endometriosis and the involvement of nuclear estrogen receptors (ER) and G-coupled protein estrogen receptor (GPER)-1 RESULTS: Upstream stimulating factor 2 protein contents were higher in mid (USF2b) and late (USF2a and USF2b) secretory phase in eutopic endometria from endometriosis than control (p &lt; 0.05). In isolated control...
Tipo: Journal article
Palavras-chave: USF2; GPER1; Endometriosis; Eutopic endometrium; Estrogen receptor; Estrogen receptor specific agonists; SF-1; P450Arom.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100056

Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells
Provedor de dados: 48
Autores: VELARDE,VICTORIA; DE LA CERDA,PAULA M; DUARTE,CLAUDIA; ARANCIBIA,FRANCISCA; ABBOTT,EDUARDO; GONZÁLEZ,ALEJANDRO; MORENO,FRANCESCA; JAFFA,AYAD A.
In addition to the induction of cell proliferation and migration, bradykinin (BK) can increase c-fos mRNA expression, activate ERK 1/2 and generate reactive oxygen species (ROS) in vascular smooth muscle cells (VSMC). It is not known, however, whether BK can induce cellular proliferation and extracellular matrix production via redox-sensitive signaling pathways. We investigated the role(s) of ROS in proliferation, migration and collagen synthesis induced by BK in VSMC derived from Sprague Dawley rat aorta. BK (10 nM) increased VSMC proliferation by 30 % (n=5); this proliferation was inhibited by the antioxidants N-acetylcysteine (20 mM) and a-lipoic acid (LA, 250 mM). In addition, BK induced an increase in cell migration and in collagen levels that were...
Tipo: Journal article
Palavras-chave: Bradykinin; Antioxidants migration signal transduction reactive oxygen species.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300007

Y Chromosomal STR Polymorphism in Northern Chinese Populations
Provedor de dados: 48
Autores: ZHENG,LIHONG; SUN,HAIMING; WANG,JINGWEI; LI,SHILIN; BAI,JING; JIN,YAN; YU,YANG; CHEN,FENG; JIN,LI; FU,SONGBIN.
Y chromosomal STRs show sufficient variability among individduals in a population and a high degree of geographical differentiation, such that their polymorphic character makes them especially suited for population genetic studies. To investígate the polymorphism of a set of 17 Y-STR loci in northern China, we genotyped the 17 Y chromosomal STR loci in a population sample of 377 unrelated males from eight ethnic populations in northern China. We calculated the haplotype frequencies, Rst value and carried out the analysis of molecular variance (AMOVA). We then drew the multidimensional scaling analysis (MDS) plot and phylogenetic tree based on the Rst value. All populations showed a high level of haplotype diversity, with low inter-population variance as...
Tipo: Journal article
Palavras-chave: China; Population; STR; Y chromosome.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400011

Polyphenols and Red Wine as Antioxidants against Peroxynitrite and other Oxidants
Provedor de dados: 48
Autores: VALDEZ,LAURA B; ÁLVAREZ,SILVIA; ZAOBORNYJ,TAMARA; BOVERIS,ALBERTO.
The antioxidant capacity of polyphenols (+)-catechin, (-)-epicatechin and myricetin, and of different types of red wines (Cabernet Sauvignon, Malbec and blended wine) was evaluated by three assays. (a) NADH oxidation by peroxynitrite (ONOO-): the ONOO- scavenging activity was higher for myricetin (IC50=35 µM) than for (+)-catechin (IC50=275 µM) and (-)-epicatechin (IC50=313 µM). (b) Peroxynitrite initiated chemiluminescence in rat liver homogenate: (-)-epicatechin (IC50=7.0 µM) and (+)-catechin (IC50=13 µM) were more potent than myricetin (IC50=20 µM) in inhibiting the chemiluminescence signal. (c) Lucigenin chemiluminescence in aortic rings: (-)-epicatechin (IC50=15 µM) and (+)-catechin (IC50=18 µM) showed higher antioxidant capacity than myricetin...
Tipo: Journal article
Palavras-chave: Antioxidant capacity; Peroxynitrite; Polyphenols; Red wines; Superoxide anion.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200014

Methodological considerations related to sleep paradigm using event related potentials
Provedor de dados: 48
Autores: IBÁÑEZ,AGUSTÍN M; SAN MARTÍN,RENÉ; HURTADO,ESTEBAN; LÓPEZ,VLADIMIR.
In the last few decades, several works on event related potentials (ERPs hereafter) during sleep have been reported. In spite of numerous studies, clear methodological rules for this kind of study are often missing, making it difficult to valorize the scope of these results. We propose here a description of methodological aspects to be considered when evaluating ERPs during sleep. The use of Rechtschaffen and Kales rules versus automatic methods is assessed, plus the additional use of certain quantitative measures. Additionally, two topics are discussed which must be controlled in ERPs sleep studies: the First Night Effect, and sleep disturbances. Better control of experimental paradigms is relevant for the growth of the neuroscience of sleep.
Tipo: Journal article
Palavras-chave: Sleep; ERP; Rechtschaffen and Kales rules; First Night Effect; Automatic sleep scoring systems.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300004

Properties of voltage-gated Ca2+ currents measured from mouse pancreatic &amp;#946;-cells in situ
Provedor de dados: 48
Autores: MEARS,DAVID; ROJAS,EDUARDO.
We used the single-microelectrode voltage-clamp technique to record ionic currents from pancreatic &amp;#946;-cells within intact mouse islets of Langerhans at 37C, the typical preparation for studies of glucose-induced "bursting" electrical activity. Cells were impaled with intracellular microelectrodes, and voltage pulses were applied in the presence of tetraethylammonium. Under these conditions, a voltage-dependent Ca2+ current (I Cav), containing L-type and non-L-type components, was observed. The current measured in situ was larger than that measured in single cells with whole-cell patch clamping, particularly at membrane potentials corresponding to the action potentials of &amp;#946;-cell electrical activity. The temperature dependence of I Cav was...
Tipo: Journal article
Palavras-chave: Islet of Langerhans; Insulin; Stimulus-secretion coupling; Ion channel; Inactivation kinetics; BAPTA.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300012

Oxidative stress is closely related to clinical severity of pre-eclampsia
Provedor de dados: 48
Autores: CHAMY,VERÓNICA M; LEPE,JAIME; CATALÁN,ÁLVARO; RETAMAL,DAVID; ESCOBAR,JORGE A; MADRID,EVA M.
Pre-eclampsia is a hypertensive disorder of pregnancy in which enzymatic antioxidant defenses fail and tissues are injured. This prospective case-control study evaluated whether pre-eclamptic women and their newborns show higher degrees of oxidative stress than normal pregnancies and sought to determine if this stress is related to clinical severity. Forty-four pre-eclamptic and thirty healthy pregnant women attending two hospitals in Valparaíso, Chile, were studied. The following plasmatic variables of antioxidant capacity were evaluated: glutathione peroxidase activity (GPx), total antioxidant capacity measured by oxidation of ABTS substrate (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), and superoxide dismutase activity (SOD). malondialdehide...
Tipo: Journal article
Palavras-chave: Pre-eclampsia; MDA; SOD; GPx; ABTS; Lipoperoxidation; Antioxidants.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200005

Modulation of spermatozoon acrosome reaction
Provedor de dados: 48
Autores: Vigil,Pilar; Orellana,Renán F; Cortés,Manuel E.
Spermatozoon acrosome reaction is an exocytotic event of the utmost importance for the development of mammalian fertilisation. Current evidence shows that the triggering of the acrosome reaction (AR) could be regulated by the action of diverse compounds, namely, metabolites, neurotransmitters and hormones. The aim of the present review is to describe the modulating effects of several compounds that have been classified as inductors or inhibitors of acrosome reaction. Among AR inductors, it is necessary to mention progesterone, angiotensin II, atrial natriuretic peptide, cathecolamines, insulin, leptin, relaxin and other hormones. Regarding the inhibitors, oestradiol and epidermal growth factor are among the substances that retard AR. It is worth mentioning...
Tipo: Journal article
Palavras-chave: Capacitation; Gamma-aminobutyric acid; Hormone modulation; Steroids; Spermatozoon acrosome reaction.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200007

Effect of exogenous nitric oxide on sperm motility in vitro
Provedor de dados: 48
Autores: Wang,Jiangtao; He,Qingliu; Yan,Xingyu; Cai,Youmei; Chen,Junyi.
BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentration and the extent of exposure to NO. To study the relationship between nitric oxide and sperm capacitationin vitro, and to provide a theoretical basis for the use of NO-related preparations in improving sperm motility for in vitro fertilization, we investigated the effects of NO concentration and time duration at these concentrations on in vitro sperm capacitation in both normal and abnormal sperm groups. We manipulated NO concentrations and the time duration of...
Tipo: Journal article
Palavras-chave: Nitric oxide; Sperm capacitation; Sperm motility; Time; Human.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100044

The complete sequence of the mitochondrial genome of the Chinook salmon, Oncorhynchus tshawytscha
Provedor de dados: 48
Autores: WILHELM,VIVIAN; VILLEGAS,JAIME; MIQUEL,ÁLVARO; ENGEL,ESTEBAN; BERNALES,SEBASTIÁN; VALENZUELA,PABLO D.T.; BURZIO,LUIS O.
The complete sequence of the mitochondrial genome of Chinook salmon, Oncorhynchus tshawytscha, has been determined. The circular genome consisting of 16,644 base pairs encodes thirteen proteins, the 12S and 16S ribosomal RNAs, and 22 transfer RNAs. These genes are ordered in the same way as most other vertebrates. The nucleotide and amino acid sequences of the ribosomal RNAs and the thirteen protein-coding genes were compared with those of other salmonids such as Oncorhynchus mykiss, Salmo salar, Salvelinus fontinalis, Salvelinus alpinus and Coregonus lavaretus. The sequence features of the control region (D-loop), the origin of L-strand replication and a putative peptide codified by the 16S mitochondrial RNA are described and discussed.
Tipo: Journal article
Palavras-chave: Salmonids; Mitochondria; Genome; Peptides; 16S rRNA.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200012

Polifenoles Vegetales, Ciencia y Bienestar
Provedor de dados: 48
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200001

Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant
Provedor de dados: 48
Autores: LEMUS,DAVID; DABANCENS,ALFREDO; ILLANES,JULIO; FUENZALIDA,MARCELA; GUERRERO,ANIBAL; LÓPEZ,CLAUDIA.
Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: ß-MAAC). Methylcellulose discs instilled with 10 µl of ß-MAAC (0.08 µg/ml),10 µl of tumor supernatant(TA3ts),5 µl ß-MAAC + 5 µl TA3ts, and 10 µl of PBS as control were implanted in host chick eggs. On day 12, the grafts were removed, photographed and fixed. Sections were stained in parallel, one and three with hematoxylin-eosin, and section two by the Tunel method. The number of vessels was evaluated in a microscopic field of the CAM (2250 µm2 ). The results show that ß-MAAC produced a significant...
Tipo: Journal article
Palavras-chave: Antiangiogenesis; Betamethasone; CAM; Tumor.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300010

XLVI REUNION ANUAL DE LA SOCIEDAD DE BIOLOGIA DE CHILE XII REUNION ANUAL DE LA SOCIEDAD DE ECOLOGIA DE CHILE
Provedor de dados: 48
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300016

Activation of group II metabotropic glutamate receptors blocks zinc release from hippocampal mossy fibers
Provedor de dados: 48
Autores: Matias,Carlos M; Dionísio,Jose C; Saggau,Peter; Quinta-Ferreira,Maria Emilia.
BACKGROUND: The hippocampal CA3 area contains large amounts of vesicular zinc in the mossy fiber terminals which is released during synaptic activity, depending on presynaptic calcium. Another characteristic of these synapses is the presynaptic localization of high concentrations of group II metabotropic glutamate receptors, specifically activated by DCG-IV. Previous work has shown that DCG-IV affects only mossy fiber-evoked responses but not the signals from associational-commissural afferents, blocking mossy fiber synaptic transmission. Since zinc is released from mossy fibers even for single stimuli and it is generally assumed to be co-released with glutamate, the aim of the work was to investigate the effect of DCG-IV on mossy fiber zinc signals....
Tipo: Journal article
Palavras-chave: Zinc; Neurotransmission; Glutamatergic; Co-release; Metabotropic fluorescence; Inhibition.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100067

The action of ovarian hormones in cardiovascular disease
Provedor de dados: 48
Autores: MEDINA,RODOLFO A; ARANDA,EVELYN; VERDUGO,CESAR; KATO,SUMIE; OWEN,GARETH I.
The incidence of cardiovascular disease (CAD) differs between men and women, in part because of differences in risk factors and hormones. This sexual dimorphism means a lower incidence in atherosclerotic diseases in premenopausal women, which subsequently rises in postmenopausal women to eventually equal that of men. These observations point towards estrogen and progesterone playing a lifetime protective role against CAD in women. As exogenous estrogen and estrogen plus progesterone preparations produce significant reductions in low-density lipoprotein (LDL) cholesterol levels and significant increases in high-density lipoprotein (HDL) cholesterol, this should in theory lower the risk of CAD. However, results from oral contraceptive (OC) use and combined...
Tipo: Journal article
Palavras-chave: Hormone replacement therapy (HRT); Oral contraceptives; Atherosclerosis cardiovascular disease; Estrogen; Progestins; Venous thrombosis; Myocardial infarction.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300005

Human Sociogenetics
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
In three cities of Chile (Santiago, Valparaiso, Valdivia) the A allele and phenotype (ABO blood group) are more frequent in the higher socioeconomic strata (SES) and the O allele and phenotype are in the lower ones. This constitutes a structured sociogenetic cline (SGC). The B allele and phenotypes (B+AB) present a rather erratic or contradictory distribution among SES. This SGC was also found in England. The standard interpretation of the origin and maintenance of this SGC in Chile is founded on socio-ethno-historic-cultural and drift factors followed by socioeconomic assortative mating that has occurred since the origin of Chileans by the admixture of Europeans and Amerindians. This interpretation is insufficient to explain the coincidence of the cline...
Tipo: Journal article
Palavras-chave: ABO; Chile; England; Rh; Socioeconomic strata; Sociogenetics.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400012

Special series on trending themes in Neurobiology in Biological Research
Provedor de dados: 48
Autores: Inestrosa,Nibaldo C.; Santos,Manuel J.
Tipo: Journal article
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100019

VEINTICINCO AÑOS DE LA RED LATINOAMERICANA DE BIOLOGIA (RELAB)
Provedor de dados: 48
Autores: JEREZ,CARLOS.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200008

p53 Codon 72 Polymorphism and Risk of Cervical Cancer
Provedor de dados: 48
Autores: OJEDA,JOSÉ M; AMPUERO,SANDRA; ROJAS,PATRICIO; PRADO,RODRIGO; ALLENDE,JORGE E; BARTON,SARA A; CHAKRABORTY,RANAJIT; ROTHHAMMER,FRANCISCO.
Storey et al. (1998) implicated the proline/argine polymorphism of the codon 72 of the tumor-suppressor gene p53 in the development of cervical cancer (CC) with the observation that the p53 protein is more efficiently inactivated by the E6 oncoprotein of human papillomavirus in p53 arginine as compared with its proline isoform. These authors further noted that in the United Kingdom, individuals homozygous for the arginine allele were several times more susceptible to HPV-associated tumorigenesis that proline/arginine heterozygotes. Subsequent studies in different countries failed to unanimously confirm this association. Motivated by the high incidence of CC in Chile, we undertook a case control study obtaining the following frequencies for genotypes PP, AP...
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200017

S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
Provedor de dados: 48
Autores: Xu,Yu&amp;#8209;Dong; Wang,Yu; Yin,Lei&amp;#8209;Miao; Peng,Ling&amp;#8209;Ling; Park,Gyoung&amp;#8209;Hee; Yang,Yong&amp;#8209;Qing.
Abstract  Background  Airway remodeling is a key feature of asthma, characterized by increased proliferation of airway smooth muscle cells (ASMCs). S100A8 is a calcium&amp;#8209;binding protein with a potential to regulate cell proliferation. Here, the effect of exogenous S100A8 protein on the proliferation of ASMCs induced by platelet&amp;#8209;derived growth factor (PDGF) and the underlying molecular mechanism was investigated.  Methods  Rat ASMCs were cultured with or without a neutralizing antibody to the receptor for advanced glycation end&amp;#8209;products (RAGE), a potential receptor for S100A8 protein. Purified recombinant rat S100A8 protein was then added into the cultured cells, and the proliferation of ASMCs induced by PDGF was detected by...
Tipo: Journal article
Palavras-chave: S100A8 Airway smooth muscle cells Cell proliferation The receptor for advanced glycation end&amp;#8209; Products Platelet&amp;#8209; Derived growth factor.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100216

Homeostasis and Heterostasis: from Invariant to Dimensionless Numbers
Provedor de dados: 48
Autores: GÜNTHER,BRUNO; MORGADO,ENRIQUE; JIMÉNEZ,RAÚL F.
In the present paper we have examined the applicability of dimensionless and invariant numbers (DN &amp;amp; IN) to the analysis of the cardiovascular system of mammals, whose functions were measured at standard metabolic conditions. The calculated IN did not change when we compared these figures with those obtained in dogs while they were submitted to graded exercise on a treadmill. In both instances, rest and exercise, the constancy of the IN prevailed, in accordance with Cannon's principle of "homeostasis" (1929). On the contrary, when dogs were examined during a standardized hypovolemic shock, we observed a breakdown of the IN, and the resulting DN evolved as a reliable index of the condition of "heterostasis" as defined by H. Selye. The robustness of...
Tipo: Journal article
Palavras-chave: Dimensional analysis; Biological similarities; Allometric equations; Rest and exercise; Hemorrhagic shock; Integral feedback.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200011

Early and late molecular and morphologic changes that occur during the in vitro transformation of Trypanosoma cruzi metacyclic trypomastigotes to amastigotes
Provedor de dados: 48
Autores: CONTRERAS,VÍCTOR T; NAVARRO,MARÍA C; DE LIMA,ANA R; DURAN,FRANCY; ARTEAGA,ROSA; FRANCO,YUNAIMY.
The amastigogenesis primary of T. cruzi occurs naturally when metacyclic trypomastigotes transform into amastigotes within the cells of the mammalian host. The in vitro study of the macromolecular changes that occur over several days during the transformation process should provide significant indications of how the parasite adapts to the mammalian host environment. We show here that metacyclic trypomastigotes pre-incubated at 37&amp;deg; C in a protein-rich medium reach a high degree of transformation to amastigotes when re-incubated in the fresh medium. Giemsa-stained smears show that during the pre-incubation phase, the metacyclic trypomastigotes undergo lengthening at the posterior end and a thinning out of the entire body. SDS-PAGE analysis of...
Tipo: Journal article
Palavras-chave: Amastigogenesis; Differentiation; Macromolecular changes; Metacyclic trypomastigotes; Trypanosoma cruzi.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100008

Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates
Provedor de dados: 48
Autores: AKINPELU,DA; ADEGBOYE,MF; ADELOYE,OA; OKOH,AI.
The in vitro antibacterial activity of crude methanolic extract of the seeds of Garcinia kola was investigated. The extracts exhibited antibacterial activities with zones of inhibition ranging from 10 mm to 25 mm. The minimum inhibitory concentration of the diethyl ether fraction was between 0.313 and 5.0 mg/ml, while that of butanol fraction varied from 0.157 to 5.0 mg/ml. The butanol fraction killed about 77% of Bacillus anthracis and 79% of Escherichia coli cells within 120 min at a concentration of 5.0 mg/ml. Protein leakage from the B. anthracis and E. coli cells when exposed to the butanol and diethyl ether fractions was observed. We conclude that Garcinia kola seed extract has a broad spectrum antibacterial activity, with the butanol and diethyl...
Tipo: Journal article
Palavras-chave: Garcinia kola; Antimicrobial activity; MIC; Killing rate; Protein leakage.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300005

Integrated analyses of copy number variations and gene differential expression in lung squamous-cell carcinoma
Provedor de dados: 48
Autores: Yang,Zhao; Zhuan,Bing; Yan,Ying; Jiang,Simin; Wang,Tao.
BACKGROUND: Although numerous efforts have been made, the pathogenesis underlying lung squamous-cell carcinoma (SCC) remains unclear. This study aimed to identify the CNV-driven genes by an integrated analysis of both the gene differential expression and copy number variation (CNV). RESULTS: A higher burden of the CNVs was found in 10-50 kb length. The 16 CNV-driven genes mainly located in chr 1 and chr 3 were enriched in immune response &amp;#91;e.g. complement factor H (CFH) and Fc fragment of IgG, low affinity Ilia, receptor (FCGR3A)&amp;#93;, starch and sucrose metabolism &amp;#91;e.g. amylase alpha 2A (AMY2A)&amp;#93;. Furthermore, 38 TFs were screened for the 9 CNV-driven genes and then the regulatory network was constructed, in which the...
Tipo: Journal article
Palavras-chave: Lung squamous-cell carcinoma; Differentially expressed genes; Copy number variation; Copy number variation-driven genes.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100047

A pilot study on genetic variation in purine-rich elements in the nephrin gene promoter in type 2 diabetic patients
Provedor de dados: 48
Autores: GONZÁLEZ,RODRIGO; TIRADO,ARES; BALANDA,MONSERRAT; ALVO,MIRIAM; BARQUÍN,INÉS; DURRUTY,PILAR; LOBOS,SERGIO; SEELENFREUND,DANIELA.
Diabetic nephropathy (DN) is one of the major complications of type 2 diabetes and is associated with coronary disease. Nephrin, a protein mainly expressed in glomeruli, is decreased in DN and other kidney diseases. Since insulin levels are misregulated in type 2 diabetes, a possible connection between DN and its decreased nephrin expression could be the presence of regulatory elements responsive to insulin in the nephrin gene (NPHS1) promoter region. In this work, using bioinformatic tools, we identified a purine-rich GAGA element in the nephrin gene promoter and conducted a genomic study in search of the presence of polymorphisms in this element and its possible association with DN in type 2 diabetic patients. We amplified and sequenced a 514 bp promoter...
Tipo: Journal article
Palavras-chave: GAGA box; Gene promoter; Nephrin; Polymorphism.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400010

First report of in vitro selection of RNA aptamers targeted to recombinant Loxosceles laeta spider toxins
Provedor de dados: 48
Autores: Sapag,Amalia; Salinas-Luypaert,Catalina; Constenla-Muñoz,Carlos.
BACKGROUND: Loxoscelism is the envenomation caused by the bite of Loxosceles spp. spiders. It entails severe necrotizing skin lesions, sometimes accompanied by systemic reactions and even death. There are no diagnostic means and treatment is mostly palliative. The main toxin, found in several isoforms in the venom, is sphingomyelinase D (SMD), a phospholipase that has been used to generate antibodies intended for medical applications. Nucleic acid aptamers are a promising alternative to antibodies. Aptamers may be isolated from a combinatorial mixture of oligonucleotides by iterative selection of those that bind to the target. In this work, two Loxosceles laeta SMD isoforms, Ll1 and Ll2, were produced in bacteria and used as targets with the aim of...
Tipo: Journal article
Palavras-chave: Antivenom; Loxosceles laeta; RNA aptamers; SELEX; SMD-Ll1; SMD-Ll2; Sphingomyelinase D.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100002

In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
Provedor de dados: 48
Autores: Wu,Shao-Chin; Liu,Min.
Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely affect reproductive functioning such as oocyte maturation and sperm capacitation. Our findings indicated that three smoke components were involved in retardation of oocyte maturation in a dose-dependent manner and the lowest-observed-adverse-effect level (LOAEL) was determined to be 10-10M. However, individual smoke components administrated at the LOAEL did not attenuate oocyte maturation, demonstrating that all three toxicants were equally required for the observed growth impairment. When exposed to all three components at...
Tipo: Journal article
Palavras-chave: Cigarette smoke; Oocyte maturation; Reproductive toxicity; Sperm capacitation.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200001

CDX-2, MUC-2 and B-catenin as intestinal markers in pure mucinous carcinoma of the breast
Provedor de dados: 48
Autores: García-Labastida,Laura; Garza-Guajardo,Raquel; Barboza-Quintana,Oralia; Rodríguez-Sanchez,Irám Pablo; Ancer-Rodríguez,Jesús; Flores-Gutierrez,Juan Pablo; Gómez-Macías,Gabriela Sofía.
BACKGROUND: Pure mucinous adenocarcinoma of the breast is a rare entity characterized by the production of variable amounts of mucin comprising 1% to 6% of breast carcinomas. Some mucinous adenocarcinomas have shown expression of intestinal differentiation markers such as MUC-2. This study examines the expression of intestinal differentiation markers in this type of breast carcinoma. RESULTS: Twenty-two cases of pure mucinous adenocarcinoma of the breast were assessed. Immunochemistry was performed for beta-catenin, CDX-2 and MUC-2. All cases were positive for B-catenin. MUC-2 positivity was observed in all cases; 63. 6% were 3 plus positive. All cases were negative for CDX-2. CONCLUSIONS: These results suggest that mucinous breast carcinomas express some...
Tipo: Journal article
Palavras-chave: Breast mucinous carcinoma; Inmunohistochemical; Intestinal differentiation.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100043

Antioxidant effect of 5-amino salicylic acid on copper-mediated LDL oxidation
Provedor de dados: 48
Autores: LÓPEZ-ALARCÓN,CAMILO; SPEISKY,HERNÁN; LISSI,EDUARDO.
The antioxidant effect of 5-Aminosalicylic acid (5-ASA) on copper-mediated LDL oxidation was followed either by the emitted chemiluminiscence (CL) or by UV-vis spectroscopy. 5-ASA addition extends the lag phase in a concentration-dependent manner without changes in the rate of the process in the autoaccelerated phase. The antioxidant behavior of 5-ASA was very similar to that of Trolox, a very efficient water soluble antioxidant. The copper-binding capacity of 5-ASA was evaluated by UV-visible spectroscopy. The addition of copper to a 5-ASA solution increases the absorbance at 332 nm and generates a new band at 298 nm. These changes in the UV-vis spectra indicate formation of a complex between 5-ASA and copper. However, LDL protection by 5-ASA is unrelated...
Tipo: Journal article
Palavras-chave: 5-Aminosalicylic acid; Antioxidant; LDL oxidation.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200006

BrainTV: a novel approach for online mapping of human brain functions
Provedor de dados: 48
Autores: LACHAUX,JEAN-PHILIPPE; JERBI,KARIM; BERTRAND,OLIVIER; MINOTTI,LORELLA; HOFFMANN,DOMINIQUE; SCHOENDORFF,BENJAMIN; KAHANE,PHILIPPE.
Our understanding of the brain's functional organisation has greatly benefited from occasional exploratory sessions during electrophysiological studies, trying various manipulations of an animal's environment to trigger responses in particular neurons. Famous examples of such exploration have unveiled various unexpected response properties, such as those of mirror neurons. This approach, which relies on the possibility to test online the reactivity of precise neural populations has no equivalent so far in humans. The present study proposes and applies a radically novel framework for mapping human brain functions in ecological situations based on a combination of a) exploratory sessions, using real-time electrophysiology to formulate hypotheses about the...
Tipo: Journal article
Palavras-chave: Speech; Gamma rhythm; Beta rhythm; Brain computer interface; Neurofeedback; Neural synchronization.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500004

Fertility and life expectancy of the predator Supputius cincticeps (Heteroptera: Pentatomidae) exposed to sublethal doses of permethrin
Provedor de dados: 48
Autores: ZANUNCIO,TERESINHA V; ZANUNCIO,JOSÉ C; SERRÃO,JOSÉ E; MEDEIROS,RÔMULO S; PINON,TOBÍAS BM; SEDIYAMA,CAMILLA AZ.
The stinkbug Supputius cincticeps (Stål) (Heteroptera: Pentatomidae) can be found in agricultural and forest ecosystems feeding primarily on larvae of Coleoptera and Lepidoptera, where it can be exposed to insecticide applications. This study therefore aimed to evaluate the reproductive potential of'S. cincticeps after exposition to sublethal doses of permethrin (5.74 x 10-3, 5.74 x 10-2, 5.74 x 10-1, 5.74 and 57.44 ppb) through the use of a fertility life table. The development cycle of this predator was determined in order to calculate its net reproductive rate (R0), the infinitesimal (r m) and finite (&amp;#955;) rates of increase in addition to mean generation time (T). The net reproductive (18.31), infinitesimal (r m) (0.050) and finite (&amp;#955;)...
Tipo: Journal article
Palavras-chave: Asopinae; Biological control; Pyrethroids; Reproduction.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100005

Identification of Adenovirus 7h Heterogeneity in the E3 Region
Provedor de dados: 48
Autores: BRUZZONE,MARISOL; BARRO,MARIO; SPENCER,EUGENIO.
Adenovirus genotype 7h was previously reported to be originated from a recombination event between adenovirus genotypes 7p and 3p. Based on those findings, further characterization of other adenovirus 7h strains become important to determine whether all adenovirus 7h strains arose from a single recombinational event. To explore such a possibility, 160 clinical isolates were studied after developing a PCR assay using a primer set designed to amplify the region corresponding to E3-7,7 Kd of adenovirus ADV 7p and E3-9 Kd of adenovirus 3p. The assay was able to differentiate most of the subgenus B strains from adeno 7h with the genotype 3d. The study of several adenovirus 7h clinical isolates revealed the existence of three variants of adeno7h. One of the...
Tipo: Journal article
Palavras-chave: Adenovirus.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200010

In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica
Provedor de dados: 48
Autores: Moukette,Bruno Moukette; Pieme,Constant Anatole; Njimou,Jacques Romain; Biapa,Cabral Prosper Nya; Marco,Bravi; Ngogang,Jeanne Yonkeu.
BACKGROUND: Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC. RESULTS: This study demonstrated that M. myristica has scavenging properties against DPPH',OH',NO', and ABTS'radicals which vary in a dose...
Tipo: Journal article
Palavras-chave: Antioxidant; Radical scavenging; HPLC; Monodora myristica; Non-timber forest product.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100015

Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers
Provedor de dados: 48
Autores: VERGARA,JULIO L; DIFRANCO,MARINO.
The effects of caffeine on the process of excitation-contraction coupling in amphibian skeletal muscle fibers were investigated using the confocal spot detection technique. This method permits to carefully discriminate between caffeine effects on the primary sources of Ca2+ release at the Z-lines where the triads are located and secondary actions on other potential Ca Release sources. Our results demonstrate that 0.5 mM caffeine potentiates and prolongs localized action-potential evoked Ca2+ transients recorded at the level of the Z-lines, but that 1mM only prolongs them. The effects at both doses are reversible. At the level of the M-line, localized Ca2+ transients displayed more variability in the presence of 1 mM caffeine than in control conditions. At...
Tipo: Journal article
Palavras-chave: Excitation-contraction coupling; Caffeine; Confocal spot detection; Calcium transients; Frog skeletal muscle; Calcium microdomains.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300017

Control of dual isoforms of Ca2+ release channels in muscle
Provedor de dados: 48
Autores: RÍOS,EDUARDO; ZHOU,JINGSONG.
Here we compare excitation-contraction coupling in single muscle cells of frogs and rats. Because amphibians have isoform 3 (or b) of the ryanodine receptor/Ca2+ release channel, in addition to 1 (a), which is also present in the mammal, any extra feature present in the frog may in principle be attributed to isoform 3. Ca2+ release under voltage clamp depolarization has a peak and a steady phase in both taxonomic classes, but the peak is more marked in the frog, where the ratio of amplitudes of the two phases is voltage-dependent. This dependence is a hallmark of CICR. Confocal imaging identified Ca2+ sparks in the frog, but not in the voltage-clamped rat cells. Because Ca2+ sparks involve CICR both observations indicate that the contribution of CICR is...
Tipo: Journal article
Palavras-chave: Excitation-contraction coupling; Sarcoplasmic reticulum; Ca2+ sparks; Ryanodine receptor; Calcium-induced calcium release.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400012

Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
Provedor de dados: 48
Autores: Bae,Seunghee; Lim,Kyung Mi; Cha,Hwa Jun; An,In-Sook; Lee,Jeong Pyo; Lee,Kwang Sik; Lee,Ghang Tai; Lee,Kun Kook; Jung,Ho Jung; Ahn,Kyu Joong; An,Sungkwan.
BACKGROUND: Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is also known alopecia. Arctiin is an active lignin isolated from Arctium lappa and has anti-inflammation, anti-microbial, and anti-carcinogenic effects. In the present study, we found that arctiin exerts anti-oxidative effects on human hair dermal papilla cells (HHDPCs). RESULTS: To better understand the mechanism, we analyzed the level of hydrogen peroxide (H2O2)-induced cytotoxicity, cell death, ROS production and senescence after arctiin pretreatment of HHDPCs. The results showed that arctiin pretreatment significantly inhibited the H2O2-induced reduction in cell...
Tipo: Journal article
Palavras-chave: Dermal papilla cell; Senescence; Cell death; MicroRNA; Arctiin.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100073

Effects of Dangguibuxue Tang, a Chinese herbal medicine, on growth performance and immune responses in broiler chicks
Provedor de dados: 48
Autores: Li,Xu Ting; Wang,Bin; Li,Jin Liang; Yang,Rui; Li,Si Cong; Zhang,Min; Huang,Wei; Cao,Liang.
The effects of Dangguibuxue Tang (DBT) on growth performance and immunity response in immunosuppressed broiler chicks were investigated in this study. 240 one-d-old broiler chicks (DaHeng S01) were randomly divided into 4 groups, 2.0% DBT-treatment (A), 0.5% DBT-treatment (B), cyclophosphamide-control (C), and control group (D). From 4 d to 7 d of age, chicks in group A, B and C were given cyclophosphamide (CY) at a dosage of 100mg/kg body weight (BW) daily by intraperitoneal injection to induce immunosuppression. Chicks in group D were given an equal volume of physiological saline daily by intraperitoneal injection and considered normal chicks. Groups A and B were supplemented with 2.0% or 0.5% of DBT in the drinking water from 8 d to 42 d of age. Groups...
Tipo: Journal article
Palavras-chave: Dangguibuxue Tang; Growth performance; Immune; Chick.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200010

Comments on Professor Serani's article
Provedor de dados: 48
Autores: MATURANA-ROMESIN,HUMBERTO.
I prefer not to comment on Professor Serani's epistemological and methodological objections, as I find they do not apply to my work from the perspective of a reading that recognizes the differences between his metaphysical position and my own. Neither the introduction nor the book itself constitute a philosophical thesis, as they emerge in a metaphysical position that neither seeks nor requires transcendental arguments to justify it. It is either validated or not by the operational coherencies of the observer's life experiences. There is no doubt that I believe that the book does what it sets out to do: it shows the conditions of the constitution of living beings and the conditions of their survival.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300006

U. DE CHILE INVESTIGA CON EE.UU
Provedor de dados: 48
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100007

Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines
Provedor de dados: 48
Autores: Rivas-Aravena,Andrea; Sandino,Ana María; Spencer,Eugenio.
Aquaculture has become an important economic sector worldwide, but is faced with an ongoing threat from infectious diseases. Vaccination plays a critical role in protecting commercially raised fish from bacterial, viral and parasitic diseases. However, the production of effective vaccines is limited by the scarcity of knowledge about the immune system of fish. Improving vaccines implies using antigens, adjuvants and employing methods of administration that are more effective and less harmful to the fish. In this context, in recent year there have studies of methods of encapsulating antigens in matrices of different types to apply in fish vaccines. This work reviews the new methods to improve fish vaccines by encapsulating them in polymers and...
Tipo: Journal article
Palavras-chave: Nanoparticles; Vaccine; Aquaculture.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400012

Role of the phosphatase PP4 in the activation of JNK-1 in prostate carcinoma cell lines PC-3 and LNCaP resulting in increased AP-1 and EGR-1 activity
Provedor de dados: 48
Autores: INOSTROZA,JUAN; SÁENZ,LEONARDO; CALAF,GLORIA; CABELLO,GERTRUDIS; PARRA,EDUARDO.
The specific signaling connections between the mitogen-activated protein kinases (MAPK) such as c-Jun N-terminal kinase (JNK-1) and phosphatases PP4 and M3/6, affecting the family of early nuclear factors, is complex and remains poorly understood. JNK-1 regulates cellular differentiation, apoptosis and stress responsiveness by up-regulating early nuclear factors such as c-Jun, a member of the activating protein (AP-1) family, and the Early Growth Factor (EGR-1). C-Jun, when phosphorylated by c-Jun N-terminal kinase (JNK-1) associates with c-Fos to form the AP-1 transcription factor that activates gene expression. We have investigated the regulation of the JNK-1 kinase by co-transfecting phosphatases PP4 and M3/6 in prostate cancer cell lines PC-3 and...
Tipo: Journal article
Palavras-chave: Prostate cancer; Phosphatase PP4; Kinase JNK-1.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200006

Caveolin-1-mediated post-transcriptional regulation of inducible nitric oxide synthase in human colon carcinoma cells
Provedor de dados: 48
Autores: FELLEY-BOSCO,EMANUELA; BENDER,FLORENT; QUEST,ANDREW F.G.
Reactive oxygen species are now widely recognized as important players contributing both to cell homeostasis and the development of disease. In this respect nitric oxide (NO) is no exception. The discussion here will center on regulation of the inducible form of nitric oxide synthase (iNOS) for two reasons. First, only iNOS produces micromolar NO concentrations, amounts that are high by comparison with the picomolar to nanomolar concentrations resulting from Ca2+-controlled NO production by endothelial eNOS or neuronal nNOS. Second, iNOS is not constitutively expressed in cells and regulation of this isoenzyme, in contrast to endothelial eNOS or neuronal nNOS, is widely considered to occur at the transcriptional level only. In particular, we were...
Tipo: Journal article
Palavras-chave: Inducible nitric oxide synthase; Proteosome_mediated proteolysis; Nitroxysomes; Cytokines; Caveolae; Caveolin-1.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200007

Cell surface and gene expression regulation molecules in dystrophinopathy: mdx vs. Duchenne
Provedor de dados: 48
Autores: FADIC,RICARDO.
Duchenne muscular dystrophy (DMD) is secondary to loss-of-function mutations in the dystrophin gene. The causes underlying the progression of DMD, differential muscle involvement, and the discrepancies in phenotypes among species with the same genetic defect are not understood. The mdx mouse, an animal model with dystrophin mutation, has a milder phenotype. This article reviews the available information on expression of signaling-related molecules in DMD and mdx. Extracellular matrix proteoglycans, growth factors, integrins, caveolin-3, and neuronal nitric oxide synthase expression do not show significant differences. Calcineurin is inconsistently activated in mdx, which is associated with lack of cardiomyopathy, compared to the permanent calcineurin...
Tipo: Journal article
Palavras-chave: Dystrophinopathy; Signaling molecules; Duchenne muscular dystrophy.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400010

Regulation of cell polarity by controlled proteolytic systems
Provedor de dados: 48
Autores: Bórquez,Daniel A; González-Billault,Christian.
Epithelial and neuronal cells are highly asymmetric, with discrete regions responsible for different roles that underlie the generation of specific compartments within cells that are distinct in biochemical composition, structure, and morphology that ultimately lead to distinct functions. Controlled and specific molecular targeting and sorting have been studied to understand the generation of asymmetric domains inside cells. Recently, a new and complementary explanation has emerged to account for the generation of domains that are enriched by a subset of proteins or polarization determinants: local proteolysis. In this review, we discuss the most conspicuous proteolytic systems that may contribute to the generation of cell polarity, namely the...
Tipo: Journal article
Palavras-chave: Calpain; Neuronal polarity; Proteolytic systems; Ubiquitin proteasome.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100005

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective
Provedor de dados: 48
Autores: Carrasco,Basilio; Meisel,Lee; Gebauer,Marlene; Garcia-Gonzales,Rolando; Silva,Herman.
This review is an overview of traditional and modern breeding methodologies being used to develop new Prunus cultivars (stone fruits) with major emphasis on peach, sweet cherry and Japanese plum. To this end, common breeding tools used to produce seedlings, including in vitro culture tools, are discussed. Additionally, the mechanisms of inheritance of many important agronomical traits are described. Recent advances in stone fruit transcriptomics and genomic resources are providing an understanding of the molecular basis of phenotypic variability as well as the identification of allelic variants and molecular markers. These have potential applications for understanding the genetic diversity of the Prunus species, molecular marker-assisted selection and...
Tipo: Journal article
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300001

Microglia ­ astrocyte interaction in Alzheimer's disease: friends or foes for the nervous system?
Provedor de dados: 48
Autores: VON BERNHARDI,ROMMY; RAMIREZ,GIGLIOLA.
Brain glial cells secrete several molecules that can modulate the survival of neurons after various types of damage to the CNS. Activated microglia and astrocytes closely associate to amyloid plaques in Alzheimer Disease (AD). They could have a role in the neurotoxicity observed in AD because of the inflammatory reaction they generate. There is controversy regarding the individual part played by the different glial cells, and the interrelationships between them. Both astrocytes and microglia produce several cytokines involved in the inflammatory reaction. Moreover, the same cytokines may have different effects, depending on their concentration and the type of cells in the vicinity. In turn, the events occurring in response to injury may lead to changes in...
Tipo: Journal article
Palavras-chave: Apoptosis; SS-amyloid; Cell activation; Inflammatory process; Neurotoxicity; Senile plaques.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200017

Ultrastructural changes of erythrocytes in whole blood after exposure to prospective in silico-designed anticancer agents: a qualitative case study
Provedor de dados: 48
Autores: Repsold,Lisa; Mqoco,Thandi; Wolmarans,Elize; Nkandeu,Sandra; Theron,Joji; Piorkowski,Tomek; du Toit,Peet; van Papendorp,Dirk; Joubert,Annie Margaretha.
BACKGROUND: Novel, in silico-designed anticancer compounds were synthesized in our laboratory namely, 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10),15-tetraen-17-ol (ESE-15-ol) and 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10)16-tetraene (ESE-16). These compounds were designed to have improved bioavailability when compared to their source compound, 2-methoxyestradiol. This theoretically would be due to their increased binding affinity to carbonic anhydrase II, present in erythrocytes. Since the novel compounds under investigation are proposed to be transported within erythrocytes bound to carbonic anhydrase II, the morphological effect which they may exert on whole blood and erythrocytes is of great significance. A secondary outcome included revision of previously...
Tipo: Journal article
Palavras-chave: Whole blood; Morphology; ESE-15-ol; ESE-16; Anticancer; Erythrocytes.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100039

Antitumor Activities of Iodoacetate and Dimethylsulphoxide Against Solid Ehrlich Carcinoma Growth in Mice
Provedor de dados: 48
Autores: FAHIM,FAWZIA A  ; ESMAT,AMR Y; MADY,ESSAM A; IBRAHIM,EMAD K.
Treatment of tumor-bearing mice with LD12.5 values of iodoacetate; IAA (1.84 mg/100g b.w.) and/or dimethylsulphoxide; DMSO (350 mg/ 100g b.w.) significantly increased the cumulative mean survival time and percentage of survivors and reduced the mean tumor weight, compared to tumor-bearing controls, however, a more pronounced effect is recorded in the combined treatment. Also, an increase in the life span (ILS%) and tumor growth inhibition ratio (T/C%) are reported and amounted to 145.78 and 43.80%, 195.54 and 61.30% and 220.77 and 78.40% in IAA, DMSO and combined-treated groups, respectively. Results obtained from biochemical studies reveal that a single IAA treatment of tumor-bearing mice significantly increased the levels of plasma lactate dehydrogenase...
Tipo: Journal article
Palavras-chave: Antioxidant enzymes; Carbohydrate metabolism dimethylsulphoxide; Iodoacetate; Solid Ehrlich carcinoma.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200015

Ultrastructural changes of the egg apparatus associated with fertilisation of natural tetraploid Trifolium pratense L. (Fabaceae)
Provedor de dados: 48
Autores: BUYUKKARTAL,H. NURHAN BAKAR.
The aim of this study is to describe the ultrastructural changes of the egg apparatus associated with fertilisation of the natural tetraploid Trifolium pratense. The pollen tube enters one of the synergids through the filliform apparatus from the micropyle. Before the entry of the pollen tube into the embryo sac, one of the synergids begins to degenerate, as indicated by increased electron density and a loss of volume. This cell serves as the site of entry for the pollen tube. Following fertilization, the vacuolar organisation in the zygote changes; in addition to the large micropylar vacuole, there are several small vacuoles of varying size. Ribosomal concentration increases significantly after fertilisation. In T. pratense, ultrastructural changes...
Tipo: Journal article
Palavras-chave: Egg apparatus; Ertilisation; Trifolium pratense L.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100003

Genetic characterization of pomegranate (Punica granatum L.) genotypes by AFLP markers
Provedor de dados: 48
Autores: Ercisli,Sezai; Kafkas,Ebru; Orhan,Emine; Kafkas,Salih; Dogan,Yildiz; Esitken,Ahmet.
The Coruh Valley, located in Northeastern Turkey, is one of the most important centers of diversity in pomegranate in Turkey. In this study, we attempted to characterize 19 promising pomegranate genotypes originating from the Coruh Valley in using fluorescent dye AFLP markers and capillary electrophoresis. Four AFLP primer combinations were used, generating a total of 297 fragments, 213 of which were polymorphic (73.0%). Resolving powers of the AFLP primers ranged from 0.700 to 1.018, with a total of 3.440, while polymorphism information contents ranged from 0.707 to 0.837 with an average of 0.764. UPGMA clustering of the genotypes showed two major groups. Most of the fruit characteristics of the genotypes within the same group were variable. Therefore,...
Tipo: Journal article
Palavras-chave: Genetic diversity; AFLP; Pomegranate.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400005

50 years of amino acid hydrophobicity scales: revisiting the capacity for peptide classification
Provedor de dados: 48
Autores: Simm,Stefan; Einloft,Jens; Mirus,Oliver; Schleiff,Enrico.
BACKGROUND: Physicochemical properties are frequently analyzed to characterize protein-sequences of known and unknown function. Especially the hydrophobicity of amino acids is often used for structural prediction or for the detection of membrane associated or embedded &amp;#946;-sheets and &amp;#945;-helices. For this purpose many scales classifying amino acids according to their physicochemical properties have been defined over the past decades. In parallel, several hydrophobicity parameters have been defined for calculation of peptide properties. We analyzed the performance of separating sequence pools using 98 hydrophobicity scales and five different hydrophobicity parameters, namely the overall hydrophobicity, the hydrophobic moment for detection of...
Tipo: Journal article
Palavras-chave: Hydrophobicity scale; Transmembrane sheets; Transmembrane helix; Beta-sheet; Amino acid pattern; Alternate hydrophobicity.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100031

Patricio Zapata Ormeño, semblanza de un maestro
Provedor de dados: 48
Autores: SERANI-MERLO,ALEJANDRO.
Tipo: Journal article
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400001

Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata
Provedor de dados: 48
Autores: ORTEGA,XIMENA; PÉREZ,LUZ M.
Lemon seedlings inoculated with Alternaria alternata develop a hypersensitive response (HR) that includes the induction of Phenylalanine ammonia-lyase (PAL, E. C. 4.3.1.5) and the synthesis of scoparone. The signal transduction pathway involved in the development of this response is unknown. We used several inhibitors of the Phosphoinositide (PI) animal system to study a possible role of Inositol-1,4,5-triphosphate (IP3 ) in the transduction of the fungal conidia signal in Citrus limon. The HR was only partially inhibited by EGTA, suggesting that not only external but internal calcium as well are necessary for a complete development of the HR. In this plant system, Alternaria alternata induced an early accumulation of the second messenger IP3. When lemon...
Tipo: Journal article
Palavras-chave: Citrus limon; IP3; Phosphoinositides; Rutaceae; Signal transduction.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100014

Analysis of gene expression changes associated with human carcinoma-associated fibroblasts in non-small cell lung carcinoma
Provedor de dados: 48
Autores: Wu,Xiaofen; Ruan,Lei; Yang,Yi; Mei,Qi.
Abstract  Background  This study aimed to investigate the gene expression changes associated with carcinoma-associated fibroblasts (CAFs) involving in non-small cell lung carcinoma (NSCLC).  Methods  We downloaded the GEO series GSE22862, which contained matched gene expression values for 15 CAF and normal fibroblasts samples, and series GSE27289 containing SNP genotyping for four matched NSCLC samples. The differentially expressed genes in CAF samples were identified using the limma package in R. Then we performed gene ontology (GO) and pathway enrichment analysis and protein&amp;#8211;protein interaction (PPI) network construction using the identified DEGs. Moreover, aberrant cell fraction, ploidy, allele-specific copy number, and loss of heterozygosity...
Tipo: Journal article
Palavras-chave: Carcinoma-associated fibroblast; Non-small cell lung carcinoma; Differentially expressed genes; Allele-specific copy number; Functional analysis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100204

Diversity of foliar endophytic fungi from the medicinal plant Sapindus saponaria L . and their localization by scanning electron microscopy
Provedor de dados: 48
Autores: García,Adriana; Rhoden,Sandro A; Rubin Filho,Celso J; Nakamura,Celso V; Pamphile,João A.
Endophytic fungi inhabit vegetable tissues or organs, without causing them any harm. Endophytes can co-evolve with plant hosts and possess species-specific interactions. They can protect the plant from insect attacks and diseases, and are also able to produce substances of biotechnological interest. In folk medicine, the bark, roots and fruits of Sapindus saponaria is used to produce substances with anxiolytic, astringent, diuretic and expectorant properties, as well as tonics, blood depuratives and cough medicine. This study evaluated the diversity of endophytic fungi present in the leaves of S. saponaria L. and observed the colonization of host plants by endophytes, using light and scanning electron microscopy. We verified that these fungi are found in...
Tipo: Journal article
Palavras-chave: Endophyte; Light microscopy; Phylogenetic analysis; SEM.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200006

Insulin signaling proteins in pancreatic islets of insulin-resistant rats induced by glucocorticoid
Provedor de dados: 48
Autores: De Paula,Flávia MM; Boschero,Antonio C; Carneiro,Everardo M; Bosqueiro,José R; Rafacho,Alex.
Chronic administration of glucocorticoids induces insulin resistance that is compensated by an increase in p-cell function and mass. Since insulin signaling is involved in the control of p-cell function and mass, we investigated the content of insulin pathway proteins in pancreatic islets. Rats were made insulin resistant by daily administration of dexamethasone (1mg/kg, b.w., i.p.) for 5 consecutive days (DEX), whilst control rats received saline (CTL). Circulating insulin and insulin released from isolated islets were measured by radioimmunoassay whereas the content of proteins was analyzed by Western blotting. DEX rats were hyperinsulinemic and exhibited augmented insulin secretion in response to glucose (P &lt; 0.01). The IRa-subunit, IRS-1, Shc, AKT,...
Tipo: Journal article
Palavras-chave: Dexamethasone; Glucocorticoid; Insulin resistance; Insulin signaling; Pancreatic islets.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300006

Discurso Chile-CIENCIA 2000
Provedor de dados: 48
Autores: GOLES CHACC,ERIC.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000100002

Ethanol Intake during Lactation Alters Milk Nutrient Composition and Growth and Mineral Status of Rat Pups
Provedor de dados: 48
Autores: AZARA,CÍNTIA R.P; MAIA,INGRID C; RANGEL,CAROLINA N; SILVA-NETO,MÁRIO A.C; SERPA,RENATA F.B; DE JESUS,EDGAR F.O; TAVARES  DO CARMO,MARIA G; FIALHO,ELIANE.
Lactating Wistar rats were fed a liquid diet containing either ethanol [ethanol-fed group (EFG)] or an isocaloric amount of carbohydrate [pair-fed group (PFG)] from day 1 postpartum up to day 14 of lactation, to investigate micro/macronutrient milk composition and the mineral status of pups. EFG presented a reduction of daily milk production and milk composition was significantly higher in protein and lower in carbohydrate, while the lipid content was similar to that of PFG. When compared to PFG, the milk of EFG had a decreased proportion of C22:6 n-3 fatty acid and an increase in medium-chain fatty acids and of several minerals. Pups of EFG showed reduced growth and a lower concentration of Cu and Sr in plasma and lower concentrations of Ca, P and Cl, and...
Tipo: Journal article
Palavras-chave: Breast milk; Ethanol; Rats; Lactation; Minerals status and milk composition.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300008

Divalent cations as modulators of neuronal excitability: Emphasis on copper and zinc
Provedor de dados: 48
Autores: DELGADO,RICARDO; VERGARA,CECILIA; WOLFF,DANIEL.
Based on indirect evidence, a role for synaptically released copper and zinc as modulators of neuronal activity has been proposed. To test this proposal directly, we studied the effect of copper, zinc, and other divalent cations on voltage-dependent currents in dissociated toad olfactory neurons and on their firing rate induced by small depolarizing currents. Divalent cations in the nanomolar range sped up the activation kinetics and increased the amplitude of the inward sodium current. In the micromolar range, they caused a dose dependent inhibition of the inward Na+ and Ca2+ currents (I Na and I Ca) and reduced de amplitude of the Ca2+-dependent K+ outward current (I Ca-K). On the other hand, the firing rate of olfactory neurons increased when exposed to...
Tipo: Journal article
Palavras-chave: Copper; Zinc; Modulators; Channels; Neuronal excitability.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100019

Effect of Psychostimulants on Distinct Attentional Parameters in Attentional Deficit/Hyperactivity Disorder
Provedor de dados: 48
Autores: LÓPEZ,JAVIER; LÓPEZ,VLADIMIR; ROJAS,DANIEL; CARRASCO,XIMENA; ROTHHAMMER,PAULA; GARCÍA,RICARDO; ROTHHAMMER,FRANCISCO; ABOITIZ,FRANCISCO.
Although there is extensive literature about the effects of stimulants on sustained attention tasks in attentional deficit/hyperactivity disorder (ADHD), little is known about the effect of these drugs on other attentional tasks involving different neural systems. In this study we measured the effect of stimulants on ADHD children, both in the electroencephalographic (EEG) activity during sustained attentional tasks and in psychometric performance during selective attentional tasks. These tasks are known to rely on different cortical networks. Our results in children medicated with 10 mg of d-amphetamine administered 60 min before the study indicate (i) a significant increase in amplitude but not latency of the P300 component of the event-related potential...
Tipo: Journal article
Palavras-chave: Attentional deficit; EEG; Event-related potentials; Hyperactivity; Stroop; P300.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300010

Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
Provedor de dados: 48
Autores: CALÁBRIA,LUCIANA KAREN; TEIXEIRA,RENATA ROLAND; COELHO GONÇALVES,SYBELLI MAGDA; PASSOS LIMA,ANDREIA BARCELOS; SANTOS,ANA ALICE DINIZ DOS; MARTINS,ANTÔNIO ROBERTO; ESPINDOLA,FOUED SALMEN.
The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain.
Tipo: Journal article
Palavras-chave: Antigen retrieval; Microwave oven; Steam cooker.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002

Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria
Provedor de dados: 48
Autores: Rabelo-Gonçalves,Elizabeth M A; Nishimura,Nancy F; Murilo R Zeitune,José.
An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10(8) CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC: mice inoculated with brucella broth (control group). Animals were killed at 7, 14, 21, 28, 35 and 60 days pi and fragments of stomach and duodenum were collected, paraffin embedded and stained by hematoxylin-eosin and Giemsa. The results showed that challenged mice exhibited mild duodenitis and gastritis. In group GA, infiltration in the duodenum was lymphoplasmacytic until day 35; in group GB, it was lymphomonocytic for 60 days pi. In the stomach, H. pylori...
Tipo: Journal article
Palavras-chave: BALB/c mouse; Experimental model; Helicobacter pylori.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100012

Bioethics and animal research: A personal perspective and a note on the contribution of Fritz Jahr
Provedor de dados: 48
Autores: LOLAS,FERNANDO.
Reviewing fundamental aspects of bioethics and outlining the work of the Bioethics Program of the Pan American Health Organization, this paper draws attention to the work of a forgotten pioneer- Fritz Jahr- who coined the term bioethics in 1927 and anticipated many of the arguments and discussions now current in biological research involving animals
Tipo: Journal article
Palavras-chave: Bioethics; Biological research; Pan American Health Organization; Fritz Jahr.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100013

Imaging single-channel calcium microdomains by total internal reflection microscopy
Provedor de dados: 48
Autores: DEMURO,ANGELO; PARKER,IAN.
The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes
Tipo: Journal article
Palavras-chave: Single-channel recording; Calcium imaging; TIRFM; N-type Ca2+ channels.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025

SHh activity and localization is regulated by perlecan
Provedor de dados: 48
Autores: Palma,Verónica; Carrasco,Héctor; Reinchisi,Gisela; Olivares,Gonzalo; Faunes,Fernando; Larraín,Juan.
Proliferation and cell fate determination in the developing embryo are extrinsically regulated by multiple interactions among diverse secreted factors, such as Sonic Hedgehog (SHh), which act in a concentration-dependent manner. The fact that SHh is secreted as a lipid-modified protein suggests the existence of a mechanism to regulate its movement across embryonic fields. We have previously shown that heparan sulfate proteoglycans (HSPGs) are required for SHh binding and signalling. However, it was not determined which specific HSPG was responsible for these functions. Here we evaluated the contribution of perlecan on SHh localization and activity. To understand the mechanism of action of perlecan at the cellular level, we studied the role of perlecan-SHh...
Tipo: Journal article
Palavras-chave: SHh; Morphogen gradients; Perlecan.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100008

Evaluation of phenolic profile, antioxidant and anticancer potential of two main representants of Zingiberaceae family against B164A5 murine melanoma cells
Provedor de dados: 48
Autores: Danciu,Corina; Vlaia,Lavinia; Fetea,Florinela; Hancianu,Monica; Coricovac,Dorina E; Ciurlea,Sorina A; M &amp;#350;oica,Codru&amp;#355;a; Marincu,Iosif; Vlaia,Vicentiu; Dehelean,Cristina A; Trandafirescu,Cristina.
BACKGROUND: Curcuma longa Linnaeus and Zingiber officinale Roscoe are two main representatives ofZingiberaceae family studied for a wide range of therapeutic properties, including: antioxidant, anti-inflammatory, anti-angiogenic, antibacterial, analgesic, immunomodulatory, proapoptotic, anti-human immunodeficiency virus properties and anticancer effects. This study was aimed to analyse the ethanolic extracts of Curcuma rhizome (Curcuma longa Linnaeus) and Zingiber rhizome (Zingiber officinale Roscoe) in terms of polyphenols, antioxidant activity and anti-melanoma potential employing the B164A5 murine melanoma cell line. RESULTS: In order to evaluate the total content of polyphenols we used Folin-Ciocâlteu method. The antioxidant activity of the two...
Tipo: Journal article
Palavras-chave: Curcuma longa Linnaeus; Zingiber officinale Roscoe; Polyphenols; Antioxidant; Melanoma.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100001

Effect of alternating the magnetic feld on phosphate metabolism in the nervous system of Helix pomatia
Provedor de dados: 48
Autores: Nikolic,Ljiljana M; Rokic,Milos B; Todorovic,Natasa V; Kartelija,Gordana S; Nedeljkovic,Miodrag S; Zakrzewska,Joanna S.
The effect of extremely low frequency magnetic felds (50 Hz, 0.5 mT) - ELF-MF, on phosphate metabolism has been studied in the isolated ganglions of the garden snail Helix pomatia, after 7 and 16 days of snail exposure to ELF-MF. The infuence of ELF-MF on the level of phosphate compounds and intracellular pH was monitored by 31P NMR spectroscopy. Furthermore, the activity of enzymes involved in phosphate turnover, total ATPases, Na+/K+-ATPase and acid phosphatase has been measured. The exposure of snails to the ELF-MF for the period of 7 days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes. Prolonged exposure to the ELF-MF for the period of 16 days caused a decrease of PCr and ATP levels and...
Tipo: Journal article
Palavras-chave: ELF-MF; Garden snail; 31P NMR spectroscopy; Phosphate turnover enzymes.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200012

The effects of cadmium chloride on secondary metabolite production in Vitis vinifera cv. cell suspension cultures
Provedor de dados: 48
Autores: Cetin,Emine Sema; Babalik,Zehra; Hallac-Turk,Filiz; Gokturk-Baydar,Nilgun.
BACKGROUND: Plant secondary metabolites are possess several biological activities such as anti-mutagenic, anti-carcinogenic, anti-aging, etc. Cell suspension culture is one of the most effective systems to produce secondary metabolites. It is possible to increase the phenolic compounds and tocopherols by using cell suspensions. Studies on tocopherols production by cell suspension cultures are seldom and generally focused on seed oil plants. Although fresh grape, grape seed, pomace and grape seed oil had tocopherols, with our best knowledge, there is no research on tocopherol accumulation in the grape cell suspension cultures. In this study, it was aimed to determine the effects of cadmium chloride treatments on secondary metabolite production in cell...
Tipo: Journal article
Palavras-chave: Cadmium chloride; Cell; Secondary metabolite.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100047

Local regulation of the axonal phenotype, a case of merotrophism
Provedor de dados: 48
Autores: COURT,FELIPE A; ÁLVAREZ,JAIME.
In this essay, we show that several anatomical features of the axon, namely, microtubular content, caliber and extension of sprouts, correlate on a local basis with the particular condition of the glial cell, i.e., the anatomy of axons is dynamic, although it is seen usually in its `normal' state. The occurrence of ribosomes and messenger RNAs in the axon suggests that axoplasmic proteins are most likely synthesized locally, at variance with the accepted notion that they are supplied by the cell body. We propose that the supporting cell (oligodendrocyte or Schwann cell) regulates the axonal phenotype by fine-tuning the ongoing axonal protein synthesis.
Tipo: Journal article
Palavras-chave: Axon; Protein synthesis; Nerve regeneration; Schwann cell; Wld s.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400009

Circadian chronotypes among wild-captured west Andean octodontids
Provedor de dados: 48
Autores: OCAMPO-GARCÉS,ADRIÁN; MENA,WILSON; HERNÁNDEZ,FELIPE; CORTÉS,NELSON; PALACIOS,ADRIÁN G.
Rest activity pattern was studied in wild-captured males of Octodon degus (n=9), Octodon bridgesi (n=3), and Spalacopus cyanus (n=6) (Rodentia: Octodontidae). Ten-minute resolution actograms were constructed from data obtained by an automated acquisition system. After two months of habituation to a stable light-dark schedule, recordings were performed in isolation chambers under a 12: 12 Light Dark schedule. A free-running period (constant darkness) was recorded for O. bridgesi and S. cyanus. O. degus displayed a crepuscular pattern of rest activity rhythm. Entrained O. bridgesi and S. cyanus displayed nocturnal preference, with rest anticipating light phase and without crepuscular activity bouts. Under constant darkness, active phase occurred at...
Tipo: Journal article
Palavras-chave: Circadian rhythms; Chronotype; Octodon; Octodontids; Spalacopus cyanus.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200003

Cyclosporin A-treated Dendritic Cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory T cell proliferation
Provedor de dados: 48
Autores: Pino-Lagos,Karina; Michea,Paula; Sauma,Daniela; Alba,Andrea; Morales,Jorge; Bono,María Rosa; Fierro,Alberto; Rosemblatt,Mario.
One of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (T REG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect T REG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled T REG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and...
Tipo: Journal article
Palavras-chave: CD4+CD25+ regulatory T cell; Cyclosporine A; Dendritic cells; Transplant tolerance.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300010

The normal chain length distribution of the O antigen is required for the interaction of Shigella flexneri 2a with polarized Caco-2 cells
Provedor de dados: 48
Autores: Hoare,Anilei; Bravo,Denisse; Martinic,Mara; Valvano,Miguel A; Contreras,Inés; Álvarez,Sergio A.
Shigella flexneri causes bacillary dysentery in humans. Essential to the establishment of the disease is the invasion of the colonic epithelial cells. Here we investigated the role of the lipopolysaccharide (LPS) O antigen in the ability of S. flexneri to adhere to and invade polarized Caco-2 cells. The S. flexneri 2a O antigen has two preferred chain lengths: a short O antigen (S-OAg) regulated by the WzzB protein and a very long O antigen (VL-OAg) regulated by Wzz pHS2. Mutants with defined deletions of the genes required for O-antigen assembly and polymerization were constructed and assayed for their abilities to adhere to and enter cultured epithelial cells. The results show that both VL- and S-OAg are required for invasion through the basolateral cell...
Tipo: Journal article
Palavras-chave: Shigella; Epithelial cells; O antigen.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100003

OSVALDO ÁLVAREZ RECUERDA A SU PROFESOR
Provedor de dados: 48
Autores: ÁLVAREZ,OSVALDO.
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200002

Scavenger Receptors and Atherosclerosis
Provedor de dados: 48
Autores: RIGOTTI,ATTILIO.
Scavenger receptors were discovered as cell surface proteins capable of binding and internalization of modified lipoproteins. These receptors exhibit a broad ligand binding specificity including potential physiological and pathophysiological ligands other than modified lipoproteins. Different classes of scavenger receptors have been identified, and their relevance in normal and pathological conditions is under investigation. Recent in vitro and in vivo studies strongly support the role of class A and class B scavenger receptors in lipid transport atherogenesis
Tipo: Journal article
Palavras-chave: Atherosclerosis; Cholesterol metabolism; Lipoprotein; Scavenger receptor; Selective cholesterol uptake.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200009

In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane
Provedor de dados: 48
Autores: Manjunathan,Reji; Ragunathan,Malathi.
BACKGROUND: Leptin, the cytokine produced by white adipose tissue is known to regulate food energy homeostasis through its hypothalamic receptor. In vitro studies have demonstrated that leptin plays a major role in angiogenesis through binding to the receptor Ob-R present on ECs by stimulating and initiating new capillary like structures from ECs. Various in vivo studies indicate that leptin has diverse effect on angiogenesis. A few reports have showed that leptin exerts pro angiogenic effects while some suggested that it has antiangiogenic potential. It is theoretically highly important to understand the effect of leptin on angiogenesis to use as a therapeutic molecule in various angiogenesis related pathological conditions. Chicken chorio allantoic...
Tipo: Journal article
Palavras-chave: Angiogenesis; HRL; Human Recombinant Leptin; CAM; Chorioallantoic membrane; ECs; Endothelial cells; VEGF; Vascular endothelial growth factor; MMP; Matrix metalloproteinase; Blood vessels; CD34.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100029

FE DE ERRATAS
Provedor de dados: 48
Tipo: Journal article
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300013

Antioxidant activity of 50% aqueous - ethanol extract from Acantholippia deserticola
Provedor de dados: 48
Autores: MORALES,GLAUCO; PAREDES,ADRIÁN; SIERRA,PATRICIA; LOYOLA,LUIS A.
The antioxidant properties oí Acantholippia deserticola, a herb used in traditional northern Chilean medicine was studied using free radical - generating systems. The 50% aqueous - ethanol extract oí Acantholippia deserticola protected against non-enzymatic lipid peroxidation in microsomal membranes of rat, induced by an Fe++ - ascorbate system and measured spectrophotometrically by the TBARS test, and had strong free radical scavenging capacities on stable ABTS and DPPH radicáis. The results shows that the IC50 valué of the 50% aqueous - ethanolic extract oí A.deserticola is 18 ± 0.5 &amp;#956;g/mL in DPPH radical - scavenging, 15 ± 0.8 &amp;#956;g/mL in lipid peroxidation , Total Antioxidant Activity (TAA) is 0.95 mM of Trolox per mg/mL of extract. The...
Tipo: Journal article
Palavras-chave: Acantholippia deserticola; Northern Chilean medicinal plant; Phenolics; Free radicáis scavenging; Microsomal lipid peroxidation.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200004

Voluntary modulations of attention in a semantic auditory-visual matching Task: an ERP study
Provedor de dados: 48
Autores: ORTEGA,RODRIGO; LÓPEZ,VLADIMIR; ABOITIZ,FRANCISCO.
The present study explores the neural correlates of voluntary modulations of attention in an auditory-visual matching task. Visual stimuli (a female or a male face) were preceded in cióse temporal proximity by auditory stimuli consisting of the Spanish word for "man" and "woman" ("hombre" or "mujer"). In 80% of the triáis the gender of the two stimuli coincided. Participants were asked to mentally count the specific instances in which a female face appeared after hearing the word "man" (10 % of the triáis). Our results show attention-related amplitude modulation of the early visual ERP components NI and anterior P2, but also amplitude modulations of (i) the N270 potential usually associated with conflict detection, (ii) a P300 wave related to infrequency,...
Tipo: Journal article
Palavras-chave: Voluntary attention; Semantic processing; Top down control; ERP components.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400010

Association between polymorphism of the G-protein &amp;#946;3 subunit C825T and essential hypertension: an updated meta-analysis involving 36,802 subjects
Provedor de dados: 48
Autores: Zheng,Huan; Xu,Huifeng; Cui,Bin; Xie,Nanzi; Wang,Zhi; Luo,Ming.
Purpose: The G-protein &amp;#946;3-subunit gene C825T polymorphism (GNB3-C825T) has been reported to be associated with essential hypertension (EH), but results from previous studies are conflicting. The present study aimed at investigating the association between this polymorphism and risk of EH using a meta-analysis on the published studies. Materials and Methods: PubMed, Embase, CBM (China Biological Medicine Database), Wanfang and VIP databases were searched to identify eligible studies published in English and Chinese before March 2013. Data were extracted using standardized methods. The association was assessed by the odds ratio (OR) with 95% confidence intervals (CI). Begg's test was used to measure publication bias. Results: A total of 40...
Tipo: Journal article
Palavras-chave: GTP-Binding Proteins; Polymorphism; Genetic hypertension; Meta-Analysis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300007

Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle
Provedor de dados: 48
Autores: CORONADO,ROBERTO; AHERN,CHRIS A; SHERIDAN,DAVID C; CHENG,WEIJUN; CARBONNEAU,LEAH; BHATTACHARYA,DIPANKAR.
Molecular understanding of the mechanism of excitation-contraction (EC) coupling in skeletal muscle has been made possible by cultured myotube models lacking specific dihydropyridine receptor (DHPR) subunits and ryanodine receptor type 1 (RyR1) isoforms. Transient expression of missing cDNAs in mutant myotubes leads to a rapid recovery, within days, of various Ca2+ current and EC coupling phenotypes. These myotube models have thus permitted structure-function analysis of EC coupling domains present in the DHPR controlling the opening of RyR1. The purpose of this brief review is to highlight advances made by this laboratory towards understanding the contribution of domains present in a1S and b1a subunits of the skeletal DHPR to EC coupling signaling. Our...
Tipo: Journal article
Palavras-chave: L-type Ca2+ channel; Ca2+ transients; Confocal imaging; CDNA expression; B1 KO myotubes; DHPR voltage sensor.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400010

Possible roles of the hereditary hemochromatosis protein, HFE, in regulating cellular iron homeostasis
Provedor de dados: 48
Autores: ENNS,CAROLINE A.
Hereditary hemochromatosis (HH) is the most common inherited disorder in people of Northern European descent. Over 83% of the cases of HH result from a single mutation of a Cys to Tyr in the HH protein, HFE. This mutation causes a recessive disease resulting in an accumulation of iron in selected tissues. Iron overload damages these organs leading to cirrhosis of the liver, diabetes, cardiomyopathy, and arthritis. The mechanism by which HFE influences iron homeostasis in cells and in the body remains elusive. Lack of functional HFE in humans produces the opposite effects in different cell types in the body. In the early stages of the disease, Kupffer cells in the liver and enterocytes in the intestine cells are iron depleted and have low intracellular...
Tipo: Journal article
Palavras-chave: Hereditary hemochromatosis; HFE; Iron overload; Iron homeostasis.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100013

A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans
Provedor de dados: 48
Autores: RIVAS,MARIELLA; SEEGER,MICHAEL; HOLMES,DAVID S; JEDLICKI,EUGENIA.
The genome of the acidophilic, proteobacterium Acidithiobacillus ferrooxidans, contains linked but divergently oriented genes, termed afeI and afeR, whose predicted protein products are significantly similar to the LuxI and LuxR families of proteins. A possible promoter and Lux box are predicted upstream of afeI. A cloned copy of afeI, expressed in E. coli, encodes an enzyme that catalyzes the production of a diffusible compound identified by gas chromatography and mass spectrometry as an unsubstituted N-acyl homoserine lactone (AHL) of chain length C14. This AHL can be detected by a reporter strain of Sinorhizobium meliloti Rm41 suggesting that it is biologically active. The reporter strain also responds to extracts of the supernatant of A. ferrooxidans...
Tipo: Journal article
Palavras-chave: A. ferrooxidans; Quorum-sensing; LuxI and LuxR; Lux box; Homoserine lactone.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200018

Stress tolerant virulent strains of Cronobacter sakazakii from food
Provedor de dados: 48
Autores: Fakruddin,Md; Rahaman,Mizanur; Ahmed,Monzur Morshed; Hoque,Md Mahfuzul.
BACKGROUND: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was to isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh. RESULT: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical characteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp. specific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately high antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates were capable of producing biofilm (strong biofilm producer), extracellular protease and...
Tipo: Journal article
Palavras-chave: Cronobacter; Food; Virulent; Bangladesh.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100057

Identification of functionally important acidic residues in transducin by group-specific labeling
Provedor de dados: 48
Autores: KOSOY,ANA; MÖLLER,CAROLINA; PERDOMO,DEISY.
Transducin (T), a GTP-binding protein involved in phototransduction of rod photoreceptor cells, is a heterotrimer arranged as two units, the a-subunit (Ta) and the bg-complex (Tbg). The role of the carboxyl groups in T was evaluated by labeling with N, N'-dicyclohexylcarbodiimide (DCCD) and 1-ethyl 3-(3-dimethylaminopropyl) carbodiimide (EDC). Only a minor effect on the binding of b, g-imido guanosine 5'-triphosphate (GMPpNp) to T was observed in the presence of the hydrophobic carbodiimide, DCCD. Similarly, the GMPpNp binding activity of the reconstituted holoenzyme was not significantly affected when Ta was combined with DCCD-treated Tbg. However, the binding of guanine nucleotides to the reconstituted T was ~50% inhibited when DCCD-labeled Ta was...
Tipo: Journal article
Palavras-chave: Chemical modification; G-protein-coupled signaling; Group-specific labeling; Transducin; Visual process.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300010

Apoptosis in HEp-2 cells infected with Ureaplasma diversum
Provedor de dados: 48
Autores: Amorim,Aline Teixeira; Marques,Lucas Miranda; Santos,Angelita Maria Oliveira Gusmão; Martins,Hellen Braga; Barbosa,Maysa Santos; Rezende,Izadora Souza; Andrade,Ewerton Ferraz; Campos,Guilherme Barreto; Lobão,Tássia Neves; Cortez,Beatriz Araujo; Monezi,Telma Alvez; Machado-Santelli,Glaucia Maria; Timenetsky,Jorge.
BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and...
Tipo: Journal article
Palavras-chave: Ureaplasma diversum; Invasion; Apoptosis; HEp-2 cells.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100038

PROGRAMA BIBLIOTECA CIENTIFICA ELECTRONICA EN LINEA, SCIELO-CHILE: UNA NUEVA FORMA DE ACCEDER A LA LITERATURA CIENTIFICA NACIONAL
Provedor de dados: 48
Autores: Prat,Anna María.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200003

Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet
Provedor de dados: 48
Autores: Hernández-Salinas,Romina; Decap,Valerie; Leguina,Alberto; Cáceres,Patricio; Perez,Druso; Urquiaga,Ines; Iturriaga,Rodrigo; Velarde,Victoria.
BACKGROUND: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet. METHODS: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index...
Tipo: Journal article
Palavras-chave: Insulin resistance; Oxidative stress; Metabolic syndrome; Wine grape powder.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100053

The proapoptotic activity of C-terminal domain of apoptosis-inducing factor (AIF) is separated from its N-terminal
Provedor de dados: 48
Autores: ZHANG,YONG; HAN,TAO; ZHU,QING; ZHANG,WEI; BAO,WEI; FU,HAI-JING; YANG,JIE; HUANG,XIAO-JUN; WEI,JUN-XIA; MENG,YAN-LING; ZHAO,JING; CAO,YUN-XIN; JIA,LIN-TAO; YANG,AN-GANG.
Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein that mediates both NADH-oxidizing and caspase-independent apoptosis. Further, the proapoptotic activity of AIF is located in the C-terminus of AIF, although the precise minimum sequence responsible for apoptosis induction remains to be investigated. In the present study, we generated two truncated AIFs, AIF&amp;#916;1-480-FLAG, which is a FLAG-tagged C-terminal peptide comprising amino acids from 481 to 613, and AIF360-480 containing amino acids from 360 to 480 of AIF. We used confocal microscopy to demonstrate that both the truncated proteins are expressed and located in the cytoplasm of transfected cells. AIF&amp;#916;1-480 but not AIF360-480 induces apoptosis in transfected cells. We also...
Tipo: Journal article
Palavras-chave: Apoptosis inducing factor (AIF); Apoptosis; Cytochrome c; Mitochondria; HER2.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200014

Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
Provedor de dados: 48
Autores: Pan,Zeya; Pan,Hao; Zhang,Jin; Yang,Yun; Liu,Hui; Yang,Yuan; Huang,Gang; Ni,Junsheng; Huang,Jian; Zhou,Weiping.
BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after...
Tipo: Journal article
Palavras-chave: Ubiquitin Specific Peptidase 39; Lentivirus; Human hepatocellular carcinoma; Cell proliferation; Cell cycle.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100018

TGF-&amp;#946;1 expression is associated with invasion and metastasis of intrahepatic cholangiocarcinoma
Provedor de dados: 48
Autores: Chen,Yijie; Ma,Lin; He,Qian; Zhang,Shaobo; Zhang,Chenghua; Jia,Wei.
BACKGROUND: Transforming growth factor (TGF)-&amp;#946; is involved in many physiologic processes, it often promotes metastasis, and its high expression is correlated with poor prognosis. In the present study, we analyzed the correlation between transforming growth factor beta 1 (TGF-&amp;#946;1) expression and prognosis in intrahepatic cholangiocarcinoma RESULTS: We examined the expression of TGF-&amp;#946; 1 in 78 intrahepatic cholangiocarcinomas by immunohistochemistry and correlated the expression with clinicopathological parameters. TGF-&amp;#946;1 was expressed in 37 of 78 (47.4 %) intrahepatic cholangiocarcinomas. The expression of TGF-&amp;#946; 1 was significantly correlated with lymph node metastasis, distant metastasis, and tumour recurrence....
Tipo: Journal article
Palavras-chave: Intrahepatic cholangiocarcinoma Transforming growth factor beta 1 (TGF&amp;#946; -1) Prognosis.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100026

Toxic cyanobacteria strains isolated from blooms in the Guadiana River (southwestern Spain)
Provedor de dados: 48
Autores: MORENO,ISABEL M; PEREIRA,PAULO; FRANCA,SUSANA; CAMEÁN,ANA.
This paper describes the occurrence of toxic cyanobacteria along the Guadiana River over its course between Mérida and Badajoz (Extremadura, Spain). Water sampling for phytoplankton quantification and toxin analysis was carried out regularly between 1999 and 2001 in six different locations, including two shallow, slow-flowing river sites, two streamed river sites and two drinking water reservoirs. The cyanobacterial community differed significantly between these locations, especially during the summer. The predominant genera were Microcystis, Oscillatoria, Aphanizomenon and Anabaena. Using an ELISA assay the total microcystin contents of natural water samples from the most eutrophic locations ranged from 0.10 _ 21.86 mg mcyst-LR equivalent·L-1 in...
Tipo: Journal article
Palavras-chave: Cyanobacteria; Microcystins; Guadiana; Spain.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300006

I Reunión regional de la red SciELO
Provedor de dados: 48
Autores: CAMARGO,PATRICIA.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300004

The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis
Provedor de dados: 48
Autores: FELMER,RICARDO N; CLARK,JOHN A.
The feasibility of ablating differentiated adipocytes and the mechanism of cell ablation with a suitable prodrug activating system is described. The system is based on the use of E. coli nitroreductase (NTR) enzyme that activates certain nitro compounds, such as the antitumor drug CB1954, into cytotoxic DNA interstrand cross-linking agents. Differentiated preadipocyte cells (3T3L1) transfected with an aP2 driven nitroreductase construct were efficiently killed after incubation with medium containing the prodrug CB1954, while untransfected cells were not affected. It was demonstrated that the mechanism of cell ablation is apoptosis and that the system has a bystander effect mediated by a toxic metabolite of the prodrug. The described system should provide a...
Tipo: Journal article
Palavras-chave: Genetic ablation; NTR/CB1954; Adipocytes; Differentiation.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300009

Antitumor compounds from Streptomyces sp. KML-2, isolated from Khewra salt mines, Pakistan
Provedor de dados: 48
Autores: Aftab,Usman; Zechel,David L.; Sajid,Imran.
BACKGROUND: Actinomycetes are gram positive bacteria with high G + C content in their DNA and are capable of producing variety of secondary metabolites. Many of these metabolites possess different biological activities and have the potential to be developed as therapeutic agents. The aim of the present study was to screen actinomycetes inhabiting halophilic environment such as Khewra salt mines present in Pakistan for cytotoxic and antitumor compounds RESULTS: An actiomycetes strain designated as Streptomyces sp. KML-2 was isolated from a saline soil of Khewra salt mines, Pakistan. The strain Streptomyces sp. KML-2 showed 84 % cytotoxic activity against larvae of Artemiasalina. In the screening phase, the strain exhibited significant antitumor activity...
Tipo: Journal article
Palavras-chave: Streptomyces sp KML-2 Antitumor activity Chromomycin SA 1-(1 H-indol-3-yl)-propane-1; 2; 3-triol.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100058

Non-selective cation channels and oxidative stress- induced cell swelling
Provedor de dados: 48
Autores: SIMON,FELIPE; VARELA,DIEGO; RIVEROS,ANA; EGUIGUREN,ANA LUISA; STUTZIN,ANDRES.
Necrosis is considered as a non-specific form of cell death that induces tissue inflammation and is preceded by cell swelling. This increase in cell volume has been ascribed mainly to defective outward pumping of Na+ caused by metabolic depletion and/or to increased Na+ influx via membrane transporters. A specific mechanism of swelling and necrosis driven by the influx of Na+ through nonselective cation channels has been recently proposed (&lt;A HREF="#2"&gt;Barros et al., 2001a&lt;/A&gt;). We have characterized further the properties of the nonselective cation channel (NSCC) in HTC cells. The NSCC shows a conductance of ~18 pS, is equally permeable to Na+ and K+, impermeant to Ca2+, requires high intracellular Ca2+ as well as low intracellular ATP for...
Tipo: Journal article
Palavras-chave: Cell death; Cell Volume; Nonselective cation channels; Flufenamic acid.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200013

Programmed cell death 5 transgenic mice attenuates adjuvant induced arthritis by 2 modifying the T lymphocytes balance
Provedor de dados: 48
Autores: Yuan,Feng; Wang,Junfeng; Zhang,Keshi; Li,Zhao; Guan,Zhenpeng.
Abstract  Background Programmed cell death 5 (PDCD5) is an apoptosis-related gene cloned from TF-1 cells whose primary biological functions are to promote apoptosis and immune regulation. The effects and mechanisms exerted by key mediators of arthritic inflammation remain unclear in PDCD5 transgenic (PDCD5 tg) mice.  Results In the current study, PDCD5 tg mice inhibited the progression of adjuvant-induced arthritis, specifically decreasing clinical signs and histological damage, compared with arthritis control mice. Additionally, the ratio of CD4+IFN-&amp;#947;+ cells (Th1) and CD4+IL-17A+ cells (Th17), as well as the mRNA expression of the pro-inflammatory mediators IFN-&amp;#947;, IL-6, IL-17A and TNF-&amp;#945;, were decreased in PDCD5 tg mice, while...
Tipo: Journal article
Palavras-chave: Rheumatoid arthritis; Adjuvant-induced arthritis; Programmed cell death 5; T lymphocytes; Inflammatory cytokines.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100229

Stem cells: sources and therapies
Provedor de dados: 48
Autores: Monti,Manuela; Perotti,Cesare; Del Fante,Claudia; Cervio,Marila; Redi,Carlo Alberto.
The historical, lexical and conceptual issues embedded in stem cell biology are reviewed from technical, ethical, philosophical, judicial, clinical, economic and biopolitical perspectives. The mechanisms assigning the simultaneous capacity to self-renew and to differentiate to stem cells (immortal template DNA and asymmetric division) are evaluated in the light of the niche hypothesis for the stemness state. The induction of cell pluripotency and the different stem cells sources are presented (embryonic, adult and cord blood). We highlight the embryonic and adult stem cell properties and possible therapies while we emphasize the particular scientific and social values of cord blood donation to set up cord blood banks. The current scientific and legal...
Tipo: Journal article
Palavras-chave: Embryonic stem cell; Adult stem cell; Pluripotency; Induced pluripotent stem cells; Umbilical cord blood; Spinal cord stroke; Parkinson' disease.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300002

Simultaneous presence of Paralytic and Diarrheic Shellfish Poisoning toxins in Mytilus chilensis samples collected in the Chiloe Island, Austral Chilean Fjords
Provedor de dados: 48
Autores: GARCÍA,CARLOS; MARDONES,PAMELA; SFEIR,ANDREA; LAGOS,NÉSTOR.
The study shown here provides the first indisputable evidence that shellfish can be contaminated with Paralytic Shellfish Poisoning (PSP) and Diarrheic Shellfish Poisoning (DSP) toxins during the summer season in the Southern Chilean fjords. Quantitative analysis of the simultaneous presence of PSP and DSP toxins in Mytilus chilensis samples collected in the Chiloe Island are shown. The High Performance Liquid Chromatography (HPLC) analysis with pre-column derivatization method for DSP toxins and the post-column derivatization methods for PSP toxins, both with fluorescent on-line detections, showed that both type of toxins were concentrated by the filter bivalve Mytilus chilensis in amounts above the international safe limits. The phytoplankton analysis...
Tipo: Journal article
Palavras-chave: DSP toxins; PSP toxins; Dinophysistoxin-1; Patagonia fjords; Chile.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500002

Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein
Provedor de dados: 48
Autores: VÁSQUEZ-DEL CARPIÓ,RODRIGO; MORALES,JAIME L; BARRO,MARIO; RICARDO,ALBA; SPENCER,EUGENIO.
Rotaviruses are the major cause of acute gastroenteritis in infants world-wide. The genome consists of eleven double stranded RNA segments. The major segment encodes the structural protein VP1, the viral RNA-dependent RNA polymerase (RdRp), which is a minor component of the viral inner core. This study is a detailed bioinformatic assessment of the VP1 sequence. Using various methods we have identified canonical motifs within the VP1 sequence which correspond to motifs previously identified within RdRps of other positive strand, double-strand RNA viruses. The study also predicts an overall structural conservation in the middle region that may correspond to the palm subdomain and part of the fingers and thumb subdomains, which comprise the polymerase core of...
Tipo: Journal article
Palavras-chave: Rotavirus; Genome replication; RNA polymerase; Bioinformatic analysis.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500008

Ramón Latorre De La Cruz, Premio Nacional de Ciencias Naturales 2002: "No Se Puede Hacer Ciencia en Solitario"
Provedor de dados: 48
Autores: PÉREZ GAVILÁN,PAMELA.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300002

High frequency of p 16 promoter methylation in non-small cell lung carcinomas from Chile
Provedor de dados: 48
Autores: GUZMAN,LEDA M; KORIYAMA,CHIHAYA; AKIBA,SUMINORI; EIZURU,YOSHITO; CASTILLO,DARWINS; CORVALAN,ALEJANDRO; AGUAYO,FRANCISCO R.
The inactivation of tumour suppressor genes by aberrant methylation of promoter regions has been described as a frequent event in neoplasia development, including lung cancer. The p16 gene is a tumour suppressor gene involved in the regulation of cell cycle progression that has been reported to be inactivated by promoter methylation in lung carcinomas at variable frequencies around the world in a smoking habit dependent manner. The purpose of this study was to investigate the methylation status of the promoter region of the p16 gene in 74 non-small cell lung carcinomas from Chile. The frequency of p16 gene inactivation by promoter methylation was determined as 79.7% (59/74). When we considered histological type, we observed that p16 promoter methylation...
Tipo: Journal article
Palavras-chave: Lung carcinoma; Methylation; Promoter region.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400011

Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
Provedor de dados: 48
Autores: Lins,Marvin Paulo; Vieira,Larissa Fernanda de Araújo; Rosa,Alfredo Aurélio Marinho; Smaniotto,Salete.
BACKGROUND: Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocytes and thymic epithelial cells (TEC) in the presence of laminin. RESULTS: GH increased thymocyte adhesion on BSA-coated and further on laminin-coated surfaces. The number of migrating cells in laminin-coated membrane was higher in GH-treated thymocyte group. In both results, VLA-6 expression on thymocytes was constant. Also, treatment with GH enhanced laminin production by TEC after 24 h in culture. However, VLA-6 integrin expression on TEC remained...
Tipo: Journal article
Palavras-chave: Thymocyte; Growth hormone; Laminin; Thymic epithelial cells.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100037

BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
Provedor de dados: 48
Autores: Wang,Limei; Wu,Xiuyin; Wang,Ruolin; Yang,Chengzhe; Li,Zhi; Wang,Cunwei; Zhang,Fenghe; Yang,Pishan.
Abstract  Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC).  Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 &amp;#956;M. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Cell apoptosis and cell cycle distribution was evaluated by Flow cytometry. Immunofluorescence staining was used to examine the expression of BRD4 in SACC cells. The quantitative real-time polymerase chain reaction (qRT-PCR) assay and western...
Tipo: Journal article
Palavras-chave: Salivary adenoid cystic carcinoma; BRD4 inhibition; Proliferation; Migration; Epithelial-mesenchymal transition.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100213

In vitro fertilization of the rock shrimp, Rhynchocinetes typus (Decapoda, Caridea): a review
Provedor de dados: 48
Autores: Dupré,Enrique M; Barros,Claudio.
This review compiles all the research done on gametes and fertilization in the rock shrimp, R. typus, and describes the sequence of events from the first gamete interaction to zygote formation and the first cleavage of the embryo, with light, fluorescence confocal and electron microscopes. Early studies showed that sperm from the vas deferens have a tack-shape with a "needle-like process" or rigid spike (RS) that extends from a semi-spherical body that contains the arms with chromatin and spines. Upon contact with seawater and by action of Na +, the arms and spines extend, producing an inverted umbrella form of the spermatozoa. The first sperm-oocyte interaction occurs between protein receptors type lectins of the sperm RS and oocyte chorion sperm ligands....
Tipo: Journal article
Palavras-chave: Spermatic ultrastructure; Oocyte; Sperm-egg interaction.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200003

Can widely used cell type markers predict the suitability of immortalized or primary mammary epithelial cell models?
Provedor de dados: 48
Autores: Ontsouka,Edgar Corneille; Bertschi,Janique Sabina; Huang,Xiao; Lüthi,Michael; Müller,Stefan; Albrecht,Christiane.
BACKGROUND: Mammary cell cultures are convenient tools for in vitro studies of mammary gland biology. However, the heterogeneity of mammary cell types, e.g., glandular milk secretory epithelial or myoepithelial cells, often complicates the interpretation of cell-based data. The present study was undertaken to determine the relevance of bovine primary mammary epithelial cells isolated from American Holstein (bMEC US) or Swiss Holstein-Friesian (bMEC CH) cows, and of primary bovine mammary alveolar epithelial cells stably transfected with simian virus-40 (SV-40) large T-antigen (MAC-T) for in vitro analyses. This was evaluated by testing their expression pattern of cytokeratin (CK) 7, 18, 19, vimentin, and &amp;#945;-smooth muscle actin (&amp;#945;-SMA....
Tipo: Journal article
Palavras-chave: Cell characterization; Flow cytometry; Gene expression; Lactation marker; Mammalian; Mammary epithelial cell.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100001

Protease activity involvement in the passage of mammalian sperm through the zona pellucida
Provedor de dados: 48
Autores: Moreno,Ricardo D; Laserre A,Andrea; Barros,Claudio.
The interaction between acrosome-reacted sperm and zona pellucida proteins is not yet fully understood. Serine protease acrosin and its zymogen proacrosin have been proposed to fulfill this function due to their capacity to bind zona pellucida glycoproteins. However, the molecular mechanism underlying this interaction has been merely speculative. Here we show that fucoidan (a sulfated polysaccharide) and solubilized zona pellucida glycoproteins, but not soybean trypsin inhibitor, are able to detach bound spermatozoa, which suggests that live sperm binds to the zona pellucida in a non-enzymatical way. Interestingly, mild proteolytic digestion with acrosin or trypsin does not modify the structure of the zona pellucida, but rather results in fewer spermatozoa...
Tipo: Journal article
Palavras-chave: Fertilization; Acrosin; Acrosome; Zona pellucida; Sperm.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200006

Methodologies for evaluation of total antioxidant activities in complex mixtures. A critical review
Provedor de dados: 48
Autores: LLESUY,SUSANA; EVELSON,PABLO; CAMPOS,ANA MARIA; LISSI,EDUARDO.
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200009

The role of thyroid hormone calorigenesis in the redox regulation of gene expression
Provedor de dados: 48
Autores: VARELA,PATRICIA; TAPIA,GLADYS; FERNÁNDEZ,VIRGINIA; VIDELA,LUIS A.
Thyroid hormone (TH; 3,3',5-triiodothyronine, T3) is required for the normal function of most tissues, with major effects on 0(2) consumption and metabolic rate. These are due to transcriptional activation of respiratory genes through the interaction of T3-liganded TH receptors with TH response elements or the activation of intermediate factors, with the consequent higher production of reactive 0(2) species (ROS) and antioxidant depletion. T3-induced oxidative stress in the liver triggers the redox upregulation of the expression of cytokines (tumor necrosis factor-á [TNF-á], interleukin-10), enzymes (inducible nitric oxide synthase, manganese superoxide dismutase), and anti-apoptotic proteins (Bcl-2), via a cascade initiated by TNF-á produced by Kupffer...
Tipo: Journal article
Palavras-chave: Thyroid hormone; Calorigenesis; Oxidative stress; Gene expression.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500004

Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption
Provedor de dados: 48
Autores: Mamun,Md. Abdullah Al; Islam,Kamrul; Alam,Md. Jahangir; Khatun,Amina; Masihul Alam,M; Al-Bari,Md. Abdul Alim; Alam,Md. Jahangir.
BACKGROUND: The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The TPF have been reported to increase osteogenic functioning in mesenchymal stem cells. However, their effects on osteoclastogenesis remain unclear. The TPF isolated from T. procumbens and investigated the effects of the TPF inhibit on osteoclast differentiation and bone resorption activities using primary osteoclastic cells. Osteoclast formation was assessed by counting the number of tartrate resistant acid phosphatase (TRAP) positive...
Tipo: Journal article
Palavras-chave: Bone resorption; Osteoclast differentiation; Pit formation; TRAP positive cells.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100051

Galectin-8 promotes migration and proliferation and prevents apoptosis in U87 glioblastoma cells
Provedor de dados: 48
Autores: Metz,Claudia; Döger,Remziye; Riquelme,Elizabeth; Cortés,Priscilla; Holmes,Christopher; Shaughnessy,Ronan; Oyanadel,Claudia; Grabowski,Catalina; González,Alfonso; Soza,Andrea.
BACKGROUND: Glioblastoma is one of the most aggressive cancers of the brain. Malignant traits of glioblastoma cells include elevated migration, proliferation and survival capabilities. Galectins are unconventionally secreted glycan-binding proteins that modulate processes of cell adhesion, migration, proliferation and apoptosis by interacting with beta-galactosides of cell surface glycoproteins and the extracellular matrix. Galectin-8 is one of the galectins highly expressed in glioblastoma cells. It has a unique selectivity for terminally sialylated glycans recently found enhanced in these highly malignant cells. A previous study in glioblastoma cell lines reported that Gal-8 coating a plastic surface stimulates two-dimensional motility. Because in other...
Tipo: Journal article
Palavras-chave: Galectin-8; Glioblastoma; Cancer; Cell cycle; Apoptosis; Proliferation; Migration.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100033

Protective roles of free avian respiratory macrophages in captive birds
Provedor de dados: 48
Autores: Mutua,Mbuvi P.; Muya,Shadrack; Gicheru,Muita M.
In the mammalian lung, respiratory macrophages provide front line defense against invading pathogens and particulate matter. In birds, respiratory macrophages are known as free avian respiratory macrophages (FARM) and a dearth of the cells in the avian lung has been purported to foreordain a weak first line of pulmonary defense, a condition associated with high mortality of domestic birds occasioned by respiratory inflictions. Avian pulmonary mechanisms including a three tiered aerodynamic filtration system, tight epithelial junctions and an efficient mucociliary escalator system have been known to supplement FARM protective roles. Current studies, however, report FARM to exhibit an exceptionally efficient phagocytic capacity and are effective in...
Tipo: Journal article
Palavras-chave: Avian lung FARM Phagocytosis PPAR &amp;#947; Ligands.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100029

Interplay between the morphometry of the lungs and the mode of locomotion in birds and mammals
Provedor de dados: 48
Autores: FIGUEROA,DANIELA; OLIVARES,RICARDO; SALABERRY,MICHEL; SABAT,PABLO; CANALS,MAURICIO.
We studied the lung diffusion parameters of two species of birds and two species of mammals to explore how structural and functional features may be paralleled by differences in life style or phylogenetic origin. We used two fast-flying species (one mammal and one bird), one running mammal and one bird species that flies only occasionally as models. The harmonic mean thickness of the air-blood barrier was very thin in the species we studied. An exception was the Chilean tinamou Notoprocta perdicaria, which only flies occasionally. It showed an air-blood barrier as thick as that of flightless Galliformes. We found that the respiratory surface density was significantly greater in flying species compared to running species. The estimated values for the oxygen...
Tipo: Journal article
Palavras-chave: Diffusing capacity; Lungs; Flight.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200010

Friend erythroleukemia cells induce angiogenesis in chick embryo chorioallantoic membrane and in human umbilical vein endothelial cells
Provedor de dados: 48
Autores: PACINI,STEFANIA; PUNZI,TIZIANA; GULISANO,MASSIMO; RUGGIERO,MARCO.
The effects of Friend erythroleukemia cells on angiogenesis were studied in chick embryo chorioallantoic membrane assay and in human umbilical vein endothelial cells. In chorioallantoic membrane assay, the conditioned medium of Friend cells stimulated in vivo angiogenesis to an extent comparable to that observed with Prostaglandin El, used as positive control. Prostaglandin El added to conditioned medium of Friend cells did not further increase angiogenesis. Conditioned medium of Friend erythroleukemia cells also stimulated proliferation of human umbilical vein endothelial cells to an extent comparable to that observed with fetal bovine serum, used as positive control. Conditioned medium and fetal bovine serum together did not affect human umbilical vein...
Tipo: Journal article
Palavras-chave: Angiogenesis; Chick embryo chorioallantoic membrane assay; Friend erythroleukemia; Human endothelial cells.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100012

Effect of salinity on the quantity and quality of carotenoids accumulated by Dunaliella salina (strain CONC-007) and Dunaliella bardawil (strain ATCC 30861) Chlorophyta
Provedor de dados: 48
Autores: GÓMEZ,PATRICIA I; BARRIGA,ALEJANDRA; CIFUENTES,ANA SILVIA; GONZÁLEZ,MARIELA A.
Dunaliella salina and D. bardawil are well-known microalgae accumulating high levels of b-carotene under growth-limiting conditions. In both taxa, this pigment is primarily composed of the isomers 9-cis and all-trans. The 9-cis b-carotene occurs only in natural sources and is the most attractive from a commercial point of view. The conditions that enhance the preferred accumulation of 9-cis b-carotene in D. salina are controversial and they have not been well established yet. This study examined the effect of salinity on the quantity and quality of total carotenoids and b-carotene isomers accumulated by D. salina (strain CONC-007) and D. bardawil (strain ATCC 30861) grown in two media with different nutritional compositions (PES and ART) and at salt...
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200008

Spatial Memory in Long Evans and Rattus Norvegicus rats
Provedor de dados: 48
Autores: BUSTOS M,GLORIA; BASOALTO B,EVELYN; PINTO-HAMUY,TERESA.
Rodents in search of food use visual environmental signals and complex spatial strategies and do not return to previously-visited locations, known as the win-shift strategy. The solution to the Olton Octagonal Maze (OOM) involves Working Memory (WM). A modified OOM was used that allows for measuring WM and Long Term Memory (LTM). The delayed spatial win-shift task consisted of a Training and Test phase separated by a delay. Prior to the Training phase, four arms were chosen at random and blocked, and food pellets were placed in the food cups of the four remaining open arms. Each rat was allowed to retrieve the pellets from the four open arms and then return to its home cage for the delay period (either 5 or 20 min). In the Test phase all 8 lanes were open,...
Tipo: Journal article
Palavras-chave: Long Term Memory; Working Memory; Olton 4X4; Long Evans Rats; Rattus Norvegicus Rats.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200009

Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis
Provedor de dados: 48
Autores: WILHELM,VIVIAN; MORALES,CARMEN; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D. T.
We have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular weights of 38,830 and 85,140. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from phylogenetically related microorganisms. Partial sequences coding the amino and carboxyl regions of MltB and a sequence of 761 base pairs encoding the amino region of TbpB have been expressed in E. coli. The strong humoral response elicited by these proteins in mouse confirmed the immunogenic properties of the recombinant...
Tipo: Journal article
Palavras-chave: Piscirickettsia salmonis; MltB; TbpB; Recombinant antigens; Recombinant vaccines.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500008

Screening the antifungal activity of essential oils against decay fungi from palmyrah leaf handicrafts
Provedor de dados: 48
Autores: Mahilrajan,Subajini; Nandakumar,Jeyarani; Kailayalingam,Robika; Manoharan,Nilushiny Aloysius; SriVijeindran,SriThayalan.
BACKGROUND: The whitish tender leaves of Palmyrah are used for making handicrafts. The problem with these articles is discolouration with time and become more brittle due to fungal attack. This could be prevented by some protective coating. Instead of expensive and harmful chemicals we decided to test natural plant essential oils to control fungal attack. Palmyrah leaf article decay fungi were isolated from two different sites of Jaffna peninsula. In this investigation Antifungal Activity of different plant essential oils from neem (Azadirachta indica), castor (Ricinus communis), citronella (Cymbopogon sp) and camphor (Cinnamomum camphora) obtained from local market have been evaluated against isolated fungi. For screening of Antifungal activity, tests and...
Tipo: Journal article
Palavras-chave: Essential oil; Growth inhibition; Minimal inhibitory concentration and Palmyrah leaf.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100035

Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride
Provedor de dados: 48
Autores: SOUZA,RAPHAEL S S; ALMEIDA,MARCELA C; MANOEL,CRISTIANO V; SANTOS-FILHO,SEBASTIÃO D; FONSECA,ADENILSON S; FILHO,MARIO BERNARDO.
Stannous chloride (SnC12) is used in nuclear medicine as a reducing agent to obtain technetium-99m-radiopharmaceuticals. It have been reported that natural products might reduce the genotoxic and cytotoxic effects related to SnC12. This work evaluated the biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli (E. coli) AB1157 (wild type) cultures submitted to the action of SnC12. E. coli AB1157 cultures (exponential growth phase) were collected by centrifugation, washed and resuspended in 0.9%NaCl. Samples were incubated in water bath shaker with: (a) SnC12 (25mg/ml), (b)Salix alba extract(11.6mg/ml) and (c)SnC12(25mg/ml) + Salix alba extract (11.6mg/ml). Incubation with 0.9% NaCl was also carried out (control). At 60...
Tipo: Journal article
Palavras-chave: Escherichia coli; Free radicals; Salix alba; Stannous chloride.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200008

Response to the gonadotropin releasing hormone agonist leuprolide in immature female sheep androgenized in utero
Provedor de dados: 48
Autores: RECABARREN,SERGIO E; SIR-PETERMANN,TERESA; LOBOS,ALEJANDRO; CODNER,ETHEL; ROJAS-GARCÍA,PEDRO P; REYES,VÍCTOR.
Similar to women with Polycystic Ovary Syndrome (PCOS), female sheep treated prenatally with testosterone (T-females) are hypergonadotropic, exhibit neuroendocrine defects, multifollicular ovarian morphology, hyperinsulinemia and cycle defects. Hypergonadotropism and multifollicular morphology may in part be due to developmentally regulated increase in pituitary responsiveness to GnRH and may culminate in increased ovarian estradiol production. In this study, we utilized a GnRH agonist, leuprolide, to determine the developmental impact of prenatal testosterone exposure on pituitary-gonadal function and to establish if prenatal exposure produces changes in the reproductive axis similar to those described for women with PCOS. Eight control and eight...
Tipo: Journal article
Palavras-chave: Androgenized females; Fetal programming; PCOS; Leuprolide; Female sheep.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200013

The Gonyautoxin 2/3 epimers reduces anal tone when injected in the anal sphincter of healthy adults
Provedor de dados: 48
Autores: GARRIDO,ROGELIO; LAGOS,NÉSTOR; LATTES,KARINNA; GARCÍA,CARLOS; AZOLAS,RODRIGO; BOCIC,GUNTHER; CUNEO,ALDO; CHIONG,HECTOR; JENSEN,CRISTIAN; HENRÍQUEZ,ANA; FERNÁNDEZ,CRISTIAN.
The primary clinical symptom of Paralytic Shellfish Poisoning is acute paralytic illness produced by paralyzing toxins. Paralytic shellfish poison is formed by a mixture of phycotoxins and their toxicity is due to its reversible binding to a receptor site on the voltage-gated sodium channel on excitable cells, thus blocking neuronal transmission. We studied the effect of the gonyautoxin 2/3 epimers by local infiltration in the anal internal sphincter of healthy voluntary adults in order to reduce anal tone. The toxin was injected after prior clinical evaluation, anoscopy and anorectal manometry. Post injection clinical examination, electromyography and anorectal manometry were performed. Resting and voluntary contraction pressures were measured and the...
Tipo: Journal article
Palavras-chave: Gonyautoxin 2/3; Phycotoxins; PSP toxins; Anal sphincter; Anal fissure.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300005

Biological screening of selected flora of Pakistan
Provedor de dados: 48
Autores: Zia-Ul-Haq,M; Raza Shah,M; Qayum,Mughal; Ercisli,Sezai.
Methanolic extracts of different parts of five medicinal plants, Ferula assafoetidaL. resin, Grewia asiaticaL. leaves, Ipomoea hederaceaJacq. seeds, Lepidium sativumL. seeds and Terminalia chebulaRetz. fruits were tested in vitrofor their cytotoxic, phytotoxic, insecticidal, nematicidal and anthelmintic activities. Ipomoea hederaceashowed very significant phytotoxic and cytotoxic activity, with 100% inhibition of Lemna minorgrowth and 100% death of Artemia salinaat concentrations of 1000 and 100 µg mL-1. Grewia asiaticaexhibited very weak activities while Lepidium sativumand Ferula assafoetidashowed moderate to good potential in all three bioassays. The results suggest screening of Ipomoea hederaceaseeds further for isolation of bioactive compounds that...
Tipo: Journal article
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400008

CALCIUM SIGNALING: A UNIVERSAL MECHANISM OF CELLULAR COMMUNICATION
Provedor de dados: 48
Tipo: Journal article
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400001

Antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of Rumexhastatus D. Don
Provedor de dados: 48
Autores: Ahmad,Sajjad; Ullah,Farhat; Ayaz,Muhammad; Zeb,Anwar; Ullah,Farman; Sadiq,Abdul.
BACKGROUND: Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or minify the excessive angiogenesis and proliferation of cells. Similarly, based the ethnomedicinal uses and literature survey, the current study is designed to explore the anti-tumor and anti-angiogenic potentials of Rumex hastatus. Anti-tumor and anti-angiogenic activities were carried out using potato-disc model and chorioallantoic membrane (CAM) assay respectively. Moreover, R. hastatus was also assessed for antibacterial activity against Agrobacterium...
Tipo: Journal article
Palavras-chave: Rumex hastatus; Agrobacterium tumefaciens; Saponins; Angiogenesis; Tumor.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100018

Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
Provedor de dados: 48
Autores: CUDDON,PAUL; BOOTMAN,MARTIN D; RICHARDS,GILLIAN R; SMITH,ALISON J; SIMPSON,PETER B; RODERICK,H LLEWELYN.
Neurons are a diverse cell type exhibiting hugely different morphologies and neurotransmitter specifications. Their distinctive phenotypes are established during differentiation from pluripotent precursor cells. The signalling pathways that specify the lineage down which neuronal precursor cells differentiate remain to be fully elucidated. Among the many signáis that impinge on the differentiation of neuronal cells, cytosolic calcium (Ca2+) has an important role. However, little is known about the nature of the Ca2+ signáis involved in fate choice in neuronal precursor cells, or their sources. In this study, we show that activation of either muscarinic or platelet-derived growth factor (PDGF) receptors induces a biphasic increase in cytosolic Ca2+ that...
Tipo: Journal article
Palavras-chave: Ca2+; PDGF; Methacholine; TRPC6; SOCE; Human neurosphere-derived cells.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200008

Genomic Approaches in Marine Biodiversity and Aquaculture
Provedor de dados: 48
Autores: Huete-Pérez,Jorge A; Quezada,Fernando.
Recent advances in genomic and post-genomic technologies have now established the new standard in medical and biotechnological research. The introduction of next-generation sequencing, NGS,has resulted in the generation of thousands of genomes from all domains of life, including the genomes of complex uncultured microbial communities revealed through metagenomics. Although the application of genomics to marine biodiversity remains poorly developed overall, some noteworthy progress has been made in recent years. The genomes of various model marine organisms have been published and a few more are underway. In addition, the recent large-scale analysis of marine microbes, along with transcriptomic and proteomic approaches to the study of teleost fishes,...
Tipo: Journal article
Palavras-chave: Aquaculture; Genomics; Marine biodiversity; Next-generation sequencing; Proteomics.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400007

XLV REUNION ANUAL DE LA SOCIEDAD DE BIOLOGIA DE CHILE
Provedor de dados: 48
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300019

A tribute to Professor Claudio Barros (1936-2008)
Provedor de dados: 48
Autores: Moreno,Ricardo; Santos,Manuel J.
Tipo: Journal article
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200001

Hematopoietic potential of mouse placenta with the application of placenta flushing
Provedor de dados: 48
Autores: WEI,BIN; YUAN,SUOMAO; GAO,YINGMAO; BING,LUJUN.
Several major vascular tissues, such as the aorta-gonad-mesonephros región (AGM), yolk sac, and fetal liver have been confirmed to possess hematopoietic function. Recently, the placenta has been demonstrated as another hematopoietic organ. However, it is not conclusive whether the placenta possesses hematopoietic ability. Therefore, we undertook a series of experiments to study the hematopoietic functions of placenta. Fetal blood circulation in the placenta is difficult to be eliminated and its interference in the study of placental hematopoiesis is inevitable. With the application of placental flushing, fetal blood contained in the placenta was eliminated. We then made the further study of placental hematopoiesis after the El2.5 placenta was flushed. Our...
Tipo: Journal article
Palavras-chave: Placenta; Hematopoiesis; HS/PCs; Mouse.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300003

Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
Provedor de dados: 48
Autores: Gao,Zhengquan; Meng,Chunxiao; Gao,Hongzheng; Zhang,Xiaowen; Xu,Dong; Su,Yuanfeng; Wang,Yuanyuan; Zhao,Yuren; Ye,Naihao.
The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (&gt; 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on...
Tipo: Journal article
Palavras-chave: Haematococcus pluvialis astaxanthin 2; 4-Epibrassinolide (EBR) carotenoid genes real-time fluorescence quantitative PCR (qRT-PCR).
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200012

Sporogenesis and development of gametophytes in an endangered plant, Tetracentron sinense Oliv
Provedor de dados: 48
Autores: Gan,Xiaohong; Xie,Dan; Cao,Lingling.
The sporogenesis and development of gametophytes in Tetracentron sinense Oliv. were studied with light microscopy. The anther has four microsporangia; its primary anther wall consists of an epidermis, an endothecium, one or two middle layers and one glandular tapetum. Simultaneous cytokinesis follows meiosis, forming a tetrahedral tetrad. Mature pollen grains are two-celled at the time of anther dehiscence. Its ovule is anatropous, bitegmic and crassinucellate; the development of the female gametophyte is of the monosporic 8-nucleate Polygonum type. Significantly, some striking features were first found in T. sinense: (1) anther dehiscence occurs soon after the endothecium fibrously thickens and the intersporangial septum degenerates; (2) tapetum...
Tipo: Journal article
Palavras-chave: Tetracentron sinense Oliv; Sporogenesis; Development of gametophytes; Natural regeneration.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400011

Mycoplasma hyorhinis and Mycoplasma fermentans induce cell apoptosis and changes in gene expression profiles of 32D cells
Provedor de dados: 48
Autores: Liu,Wenbin; Shou,Chengchao.
Infection of mycoplasmas has been linked to various human diseases including arthritis, pneumonia, infertility and cancer. While Mycoplasma hyorhinis and Mycoplasma fermentans have been detected in gastric adenocarcinomas, the mechanisms underlyine the pathogenesis are unknown. In this study, cell growth kinetics, Hoechst 33258 staining, DNA ladder assays, Western blotting analysis and cDNA microarray assays were performed to investigate the roles of M. hyorhinis and M. fermentans during infection of mammalian cells. Our data demonstrated that these mycoplasmas inhibid the growth of immortalised cell lines (32D and COS-7) ane tumor cell lines (HeLa and AGS). In addition, the infection of the 32D cell line with M. hyorhinis and M. fermentans induced...
Tipo: Journal article
Palavras-chave: Mycoplasma; Apoptosis; Gene expression profiles.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400011

Geobacillus stearothermophilus LV cadA gene mediates resistance to cadmium, lead and zinc in zntA mutants of Salmonella entérica serovar Typhimurium
Provedor de dados: 48
Autores: PÉREZ,JOSÉ M; PRADEÑAS,GONZALO A; NAVARRO,CLAUDIO A; HENRÍQUEZ,DANIEL R; PICHUANTES,SERGIO E; VÁSQUEZ,CLAUDIO C.
Salmonella entérica serovar Typhimurium cells expressing the cadA gene of Geobacillus stearothermophilus LV exhibit a hypersensitive phenotype to cadmium chloride. Deletion of the ORF STM3576 from the Salmonella genome resulted in cadmium, lead and zinc sensitivity, confirming that this ORF is a homologue of the zntA gene. The observed sensitivity was reverted upon expression of the G. stearothermophilus LV cadA gene. These results indicate that the cadA gene product is involved in Cd, Pb and Zn resistance as a classical P-type ATPase and strongly suggest that the observed hypersensitive phenotype to these metals can be related to the function of the host ·zntA gene product
Tipo: Journal article
Palavras-chave: CadA; Cadmium; Lead; P-type ATPase; Zinc; ZntA.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500009

Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia
Provedor de dados: 48
Autores: TIIDUS,PETER M; DELLER,MIRADA; BOMBARDIER,ERIC; GÜL,MUSTAFA; LIU,X. LINDA.
This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 or 72 hours post-ischemia. Control animals were sacrificed without ischemia. Plantaris and red and white gastrocneimus muscles were removed and assayed for myeloperoxidase (MPO), a marker of neutrophil infiltration, and glucose-6-phosphate dehydrogenase (G6PD) and ß-glucuronidase (&lt;img border=0 width=8 height=15 id="_x0000_i1026" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom&gt;GLU), as markers of muscle damage. Significant...
Tipo: Journal article
Palavras-chave: Ischemia; Estrogen; Muscle damage; Skeletal muscle; Neutrophils.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200011

Slow axoplasmic transport under scrutiny
Provedor de dados: 48
Autores: Court,Felipe A; Álvarez,Jaime.
The origin of axoplasmic proteins is central for the biology of axons. For over fifty years axons have been considered unable to synthesize proteins and that cell bodies supply them with proteins by a slow transport mechanism. To allow for prolonged transport times, proteins were assumed to be stable, i.e., not degraded in axons. These are now textbook notions that configure the slow transport model (STM). The aim of this article is to cast doubts on the validity of STM, as a step toward gaining more understanding about the supply of axoplasmic proteins. First, the stability of axonal proteins claimed by STM has been disproved by experimental evidence. Moreover, the evidence for protein synthesis in axons indicates that the repertoire is extensive and the...
Tipo: Journal article
Palavras-chave: Protein synthesis; Radioactive wave; Maintenance of axons; Schwann cell; Glia; Transgenic mouse.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400001

Coenzyme Q10 defects may be associated with a deficiency of Q10-independent mitochondrial respiratory chain complexes
Provedor de dados: 48
Autores: Fragaki,Konstantina; Chaussenot,Annabelle; Benoist,Jean-François; Ait-El-Mkadem,Samira; Bannwarth,Sylvie; Rouzier,Cécile; Cochaud,Charlotte; Paquis-Flucklinger,Véronique.
BACKGROUND: Coenzyme Q10 (CoQ10 or ubiquinone) deficiency can be due either to mutations in genes involved in CoQ10 biosynthesis pathway, or to mutations in genes unrelated to CoQ10 biosynthesis. CoQ10 defect is the only oxidative phosphorylation disorder that can be clinically improved after oral CoQ10 supplementation. Thus, early diagnosis, first evoked by mitochondrial respiratory chain (MRC) spectrophotometric analysis, then confirmed by direct measurement of CoQ10 levels, is of critical importance to prevent irreversible damage in organs such as the kidney and the central nervous system. It is widely reported that CoQ10 deficient patients present decreased quinone-dependent activities (segments I + III or G3P + III and II + III) while MRC activities...
Tipo: Journal article
Palavras-chave: Mitochondrial disease; CoQ10 deficiency; Respiratory chain; Spectrophotometry; LC-MSMS.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100004

Tridax procumbens flavonoids promote osteoblast differentiation and bone formation
Provedor de dados: 48
Autores: Mamun,Md. Abdullah Al; Hosen,Mohammad Jakir; Islam,Kamrul; Khatun,Amina; Masihul Alam,M.; Al-Bari,Md. Abdul Alim.
BACKGROUND: Tridaxprocumbens flavonoids (TPFs) are well known for their medicinal properties among local natives. Besides traditionally used for dropsy, anemia, arthritis, gout, asthma, ulcer, piles, and urinary problems, it is also used in treating gastric problems, body pain, and rheumatic pains of joints. TPFs have been reported to increase osteogenic functioning in mesenchymal stem cells. Our previous study showed that TPFs were significantly suppressed the RANKL-induced differentiation of osteoclasts and bone resorption. However, the effects of TPFs to promote osteoblasts differentiation and bone formation remain unclear. TPFs were isolated from Tridax procumbens and investigated for their effects on osteoblasts differentiation and bone formation by...
Tipo: Journal article
Palavras-chave: Alkaline phosphatase; Bone formation; Osteoblast differentiation; Osteocalcin.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100065

Effects of Steroidal and Non Steroidal Drugs on the Neovascularization Response Induced by Tumoral TA3 Supernatant on CAM from Chick Embryo
Provedor de dados: 48
Autores: ZÚÑIGA,JORGE; FUENZALIDA,MARCELA; GUERRERO,ANÍBAL; ILLANES,JULIO; DABANCENS,ALFREDO; DÍAZ,EUGENIA; LEMUS,DAVID.
Angiogenesis, the development of new blood vessels from the existing vascular network, may result as a consequence of the increase or decrease of proangiogenic or antiangiogenic factors, respectively. The tumor itself could up-regulate the production of angiogenic factors. Recently, we established that the steroidal drug betamethasone in low concentration inhibit the neovascularization promoted by TA3 Ts on CAM of chick embryos. We describe here the effects of the non-steroidal drug ketoprofen, alone or in association with betamethasone, on the angiogenesis promoted by TA3 Ts on CAM. The main finding reported here is that the formation of new blood vessels is strongly inhibited by low concentrations of ketoprofen. The association of both drugs produced a...
Tipo: Journal article
Palavras-chave: Angiogenesis; Antiangiogenesis; Tumor; Betamethasone; Ketoprofen.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200013

Modification of fatty acid composition in broiler chickens fed canola oil
Provedor de dados: 48
Autores: Gallardo,María A; Pérez,Druso D; Leighton,Federico M.
This article describes the possibility of modifying the composition of fat tissue in broiler chickens fed canola oil, which is high in monounsaturated fatty acids. 128 one-day old broiler chickens, randomly assigned into 4 groups of 32 chicks each, received one of four diets containing 15% oil with different percentages of canola oil (diet 1: 0% canola oil, diet 2: 5% canola oil, diet 3: 10% canola oil and diet 4: 15% canola oil), for 31 days. Each group was divided into 4 subgroups of 8 chicks. The birds were sacrificed at day 45 to obtain tissue samples. The fatty acid composition was measured in meat (legs and breasts), fat (abdominal and subcutaneous) and plasma. An increase in oleic acid (p&lt;0.01) was detected, as well as a decrease in linoleic acid...
Tipo: Journal article
Palavras-chave: Oleic acid; Meat; Monounsaturated fatty acids; Omega 9; Polyunsaturated fatty acids.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200007

Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons
Provedor de dados: 48
Autores: Codocedo,Juan Francisco; Inestrosa,Nibaldo C.
BACKGROUND: Wnt-5a is a member of the WNT family of secreted lipoglycoproteins, whose expression increases during development; moreover, Wnt-5a plays a key role in synaptic structure and function in the adult nervous system. However, the mechanism underlying these effects is still elusive. MicroRNAs (miRNAs) are a family of small non-coding RNAs that control the gene expression of their targets through hybridization with complementary sequences in the 3' UTR, thereby inhibiting the translation of the target proteins. Several evidences indicate that the miRNAs are actively involved in the regulation of neuronal function RESULTS: In the present study, we examined whether Wnt-5a modulates the levels of miRNAs in hippocampal neurons. Using PCR arrays, we...
Tipo: Journal article
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100009

Fixations of the HIV-1 env gene refute neutralism: New evidence for pan-selective evolution
Provedor de dados: 48
Autores: Valenzuela,Carlos Y; Flores,Sergio V; Cisternas,Javier.
We examined 103 nucleotide sequences of the HIV-1 env gene, sampled from 35 countries and tested: I) the random (neutral) distribution of the number of nucleotide changes; II) the proportion of bases at molecular equilibrium; III) the neutral expected homogeneity of the distribution of new fxated bases; IV) the hypothesis of the neighbor infuence on the mutation rates in a site. The expected random number of fxations per site was estimated by Bose-Einstein statistics, and the expected frequencies of bases by matrices of mutation-fxation rates. The homogeneity of new fxations was analyzed using &amp;#967;2 and trinomial tests for homogeneity. Fixations of the central base in trinucleotides were used to test the neighbor infuence on base substitutions....
Tipo: Journal article
Palavras-chave: Bose-Einstein distribution; Fxations; HIV-1 env gene; Neutral evolution; Pan-selective evolution.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200002

Genetic polymorphism of GSTM1 in women with breast cancer and interact with reproductive history and several clinical pathologies
Provedor de dados: 48
Autores: LINHARES,JOSÉ JUVENAL; GUERREIRO DA SILVA,ISMAEL DALE COTRIM; NOGUEIRA DE SOUZA,NAIARA C; COELHO NORONHA,EMMANUELLE; FERRARO,ODAIR; DE CARVALHO,CRISTINA VALLETA; CHADA BARACAT,EDMUND; FARAH BARACAT,FAUSTO.
Due to the conflicting results regarding the association between breast cancerand the GSTM1 null mutation, our aim was to research this associationin a Brazilian population and correlations withsmoking, reproductive history and several clinical pathologies. A case-control study was performed on 105 women with breast cancer and 278 controls. Extraction of DNA was accomplished according to the protocol of the GFX® kit and polymorphism analysis by the PCR technique. The control and experimental groups were compared and statistical analysis assessed by X² or Fisher's exact test. The deletion in the GSTM1 gene in the breast cancer group had a prevalence of 32 (30.4%) individuals with the presence of null mutation. In the control group, the null mutation was...
Tipo: Journal article
Palavras-chave: Breast cancer; Genetic polymorphisms; GSTM1.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200017

Divalent cation hinder the solubilization of a tubulin kinase activity from Trypanosoma cruzi epimastigotes
Provedor de dados: 48
Autores: UZCANGA,GRACIELA; GALÁN-CARIDAD,JOSÉ MANUEL; SUAREZ,KAREM NORIS; BUBIS,JOSÉ.
Trypanosoma cruzi epimastigotes were extracted under various conditions in order to examine the role of divalent cations in the solubilization of microtubule proteins. When epimastigotes were homogenized in the presence of 5 mM Mg+2 and 5 mM Ca+2, a protein kinase responsible for phosphorylating tubulin, as well as the tubulin that became phosphorylated, remained tightly associated with the parasite particulate and detergent-resistant fractions. On the contrary, tubulin kinase and its substrate were predominantly released into the parasite cytosolic and detergent-soluble fractions, when epimastigotes were extracted in the presence of 5 mM EDTA and 5 mM EGTA. These evidences demonstrated a divalent cation-dependent solubilization of the enzyme responsible...
Tipo: Journal article
Palavras-chave: Microtubules; Post-translational modification of tubulin; Protein kinase CK2; Protein phosphorylation-dephosphorylation; Signal transduction; Trypanosoma cruzi; Tubulin.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300008

The Relationship between Leaf Rolling and Ascorbate-Glutathione Cycle Enzymes in Apoplastic and Symplastic Areas of Ctenanthe setosa Subjected to Drought Stress
Provedor de dados: 48
Autores: SARUHAN,NESLIHAN; TERZI,RABIYE; SAGLAM,AYKUT; KADIOGLU,ASIM.
The ascorbate-glutathione (ASC-GSH) cycle has an important role in defensive processes against oxidative damage generated by drought stress. In this study, the changes that take place in apoplastic and symplastic ASC-GSH cycle enzymes of the leaf and petiole were investigated under drought stress causing leaf rolling in Ctenanthe setosa (Rose.) Eichler (Marantaceae). Apoplastic and symplastic extractions of leaf and petiole were performed at different visual leaf rolling scores from 1 to 4 (1 is unrolled, 4 is tightly rolled and the others are intermediate forms). Glutathione reductase (GR), a key enzyme in the GSH regeneration cycle, and ascorbate (ASC) were present in apoplastic spaces of the leaf and petiole, whereas dehydroascorbate reductase (DHAR),...
Tipo: Journal article
Palavras-chave: Ctenanthe setosa; Antioxidant enzymes; Antioxidant substances; Drought; Leaf rolling.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300006

Multiple imputation procedures allow the rescue of missing data: An application to determine serum tumor necrosis factor (TNF) concentration values during the treatment of rheumatoid arthritis patients with anti-TNF therapy
Provedor de dados: 48
Autores: SCHIATTINO,IRENE; VILLEGAS,RODRIGO; CRUZAT,ANDREA; CUENCA,JIMENA; SALAZAR,LORENA; ARAVENA,OCTAVIO; PESCE,BÁRBARA; CATALÁN,DIEGO; LLANOS,CAROLINA; CUCHACOVICH,MIGUEL; AGUILLÓN,JUAN C.
Longitudinal studies aimed at evaluating patients clinical response to specific therapeutic treatments are frequently summarized in incomplete datasets due to missing data. Multivariate statistical procedures use only complete cases, deleting any case with missing data. MI and MIANALYZE procedures of the SAS software perform multiple imputations based on the Markov Chain Monte Carlo method to replace each missing value with a plausible value and to evaluate the efficiency of such missing data treatment. The objective of this work was to compare the evaluation of differences in the increase of serum TNF concentrations depending on the ­308 TNF promoter genotype of rheumatoid arthritis (RA) patients receiving anti-TNF therapy with and without multiple...
Tipo: Journal article
Palavras-chave: Multiple imputation; Mixed model; TNF polymorphism.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100002

Homeostatic and toxic mechanisms regulating manganese uptake, retention, and elimination
Provedor de dados: 48
Autores: ROTH,JEROME A.
This review attempts to summarize and clarify our basic knowledge as to the various factors that potentially influence the risks imposed from chronic exposure to high atmospheric levels of manganese (Mn). The studies describe the interrelationship of the different systems in the body that regulate Mn homeostasis by characterizing specific, biological components involved in its systemic and cellular uptake and its elimination from the body. A syndrome known as manganism occurs when individuals are exposed chronically to high levels of Mn, consisting of reduced response speed, intellectual deficits, mood changes, and compulsive behaviors in the initial stages of the disorder to more prominent and irreversible extrapyramidal dysfunction resembling Parkinson's...
Tipo: Journal article
Palavras-chave: Manganese; Iron; Lung; Parkinson's disease; Divalent metal transporter (DMT1); Transferrin; Transferrin receptor.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100006

Effects of simvastatin on bleomycin-induced pulmonary fibrosis in female rats
Provedor de dados: 48
Autores: Tulek,Baykal; Kiyan,Esen; Kiyici,Aysel; Toy,Hatice; Bariskaner,Hulagu; Suerdem,Mecit.
Statins reduce cholesterol levels by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A reductase and have a major place in the treatment of atherosclerotic disease. Recent studies have shown anti-inflammatory properties of statins. The purpose of this study was to evaluate the anti-inflammatory effect of simvastatin on bleomycin (BLM)-induced pulmonary fibrosis in rats. A total of 31 female Sprague-Dawley rats were divided into four groups: (1) intratracheal (IT) phosphate-buffered saline (PBS) + intraperitoneal (IP) PBS (n=7); (2) IT BLM + IP PBS (n=8); (3) IT BLM + low dose (LD) simvastatin (1 mg/kg daily, n=8); (4) IT BLM + high dose (HD) simvastatin (5 mg/kg daily, n=8). Simvastatin was administered IP for 15 days, beginning 1 day prior to IT BLM. The...
Tipo: Journal article
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400003

Letters to Editor / Erratum
Provedor de dados: 48
Autores: Ntuli,Tobias.
Tipo: Journal article
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300013

How the carotid body works: Different strategies and preparations to solve different problems
Provedor de dados: 48
Autores: ZAPATA,PATRICIO; LARRAÍN,CAROLINA.
This is a review of the different experimental approaches developed to solve the problems in our progress towards a comprehensive understanding of how arterial chemoreceptors operate. An analysis is performed of the bases, advantages and limits of the following preparations: studies of ventilatory reflexes originated from carotid bodies (CBs) in the entire animal; recordings of CB chemosensory discharges in situ; CB preparations perfused in situ; CB explants in oculo; CB explants in ovo; CB preparations incubated in vitro; CB preparations superfused in vitro; CB preparations perfused and superfused in vitro; CB tissue slices in vitro; cells acutely dissociated from CBs; CB cells in tissue culture; petrosal ganglia superfused in vitro; petrosal ganglion...
Tipo: Journal article
Palavras-chave: Arterial chemoreceptors; Carotid body; Chemosensory activity; Co-cultures; Cultures; In situ; In vitro; Petrosal ganglia; Superfusion.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400002

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
Provedor de dados: 48
Autores: Mata-Miranda,Monica Maribel; Vazquez-Zapien,Gustavo Jesus; Rojas-Lopez,Marlon; Sanchez-Monroy,Virginia; Perez-Ishiwara,David Guillermo; Delgado-Macuil,Raul Jacobo.
Abstract  Background  Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.  Results  In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed...
Tipo: Journal article
Palavras-chave: Pluripotent stem cells; Differentiated kidney cells; Vibrational spectroscopy; Fourier transform infrared; Principal components analysis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100207

Compositional studies and biological activities of some mash bean (Vigna mungo (L.) Hepper) cultivars commonly consumed in Pakistan
Provedor de dados: 48
Autores: Zia-Ul-Haq,Muhammad; Ahmad,Shakeel; Bukhari,Shazia Anwer; Amarowicz,Ryszard; Ercisli,Sezai; Jaafar,Hawa ZE.
BACKGROUND: In recent years, the desire to adopt a healthy diet has drawn attention to legume seeds and food products derived from them. Mash bean is an important legume crop used in Pakistan however a systematic mapping of the chemical composition of mash bean seeds is lacking. Therefore seeds of four mash bean (Vigna mungo (L.) Hepper, family Leguminoseae) cultivars (NARC-Mash-1, NARC-Mash-2, NARC-Mash-3, NARC-Mash-97) commonly consumed in Pakistan have been analyzed for their chemical composition, antioxidant potential and biological activities like inhibition of formation of advanced glycation end products (AGE) activity and tyrosinase inhibition activity. RESULTS: The investigated cultivars varied in terms of biochemical composition to various...
Tipo: Journal article
Palavras-chave: Nutrients; Antioxidant potential; Mash bean cultivar; Pakistan.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100023

Protective effect of silk lutein on ultraviolet B-irradiated human keratinocytes
Provedor de dados: 48
Autores: Pongcharoen,Sutatip; Warnnissorn,Prateep; Lertkajornsin,Ongart; Limpeanchob,Nanteetip; Sutheerawattananonda,Manote.
Carotenoids are efficient antioxidants that are of great importance for human health. Lutein and zeaxanthin are carotinoids present in high concentrations in the human retina which are involved in the photoprotection of the human eye. Lutein may also protect the skin from ultraviolet (UV)-induced damage. The present study investigated the protective effect of lutein extracted from yellow silk cocoons of Bombyx mori on human keratinocytes against UVB irradiation. A human keratinocyte cell line and primary human keratinocytes were used to investigate the UVB protection effects of silk lutein and plant lutein. Silk lutein showed no cytotoxicity to keratinocytes. Treatment with silk lutein prior to UVB irradiation enhanced cell viability and cell...
Tipo: Journal article
Palavras-chave: Silk lutein; Keratinocytes; UV; Apoptosis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100006

Targeting and fusion proteins during mammalian spermiogenesis
Provedor de dados: 48
Autores: RAMALHO-SANTOS,JOÃO; MORENO,RICARDO D.
Regulated exocytosis is controlled by internal and external signals. The molecular machinery controlling the sorting from the newly synthesized vesicles from the Golgi apparatus to the plasma membrane play a key role in the regulation of both the number and spatial location of the vesicles. In this context the mammalian acrosome is a unique vesicle since it is the only secretory vesicle attached to the nucleus. In this work we have studied the membrane trafficking between the Golgi apparatus and the acrosome during mammalian spermiogenesis. During bovine spermiogenesis, Golgi antigens (mannosidase II) were detected in the acrosome until the late cap-phase spermatids, but are not found in testicular spermatozoa (maturation-phase spermatids). This suggests...
Tipo: Journal article
Palavras-chave: Acrosome; Spermatid; Golgi; Fertilization; Spermiogenesis.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200021

Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii
Provedor de dados: 48
Autores: LIU,BO; LIWANG,QI; RUIYANG,CHEN; SONG,WENQIN.
The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six pairs of large metacentrics and six pairs of medium-sized submetacentrics. The identification of homologous pairs is hampered by their high degree of similarity at the morphological level in each group. As one of the most extensively used methods in molecular cytogenetics producing chromosome landmarks, fluorescence in situ hybridization (FISH) has significantly facilitated karyotype construction, especially in species with morphologically similar chromosomes. This study developed a simple but effective use of combinatorial labeling probes to distinguish chromosomes of Larix principis-rupprechtii by multicolor FISH. Three highly repetitive sequences in Larix were selected:...
Tipo: Journal article
Palavras-chave: Combinatorial labeling probes; Fluorescence in situ hybridization; Karyotype; Larix principis-rupprechtii; LPD repeats; 25S and 5S rDNA.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100003

1a,25(OH)2D3 induces capacitative calcium entry involving a trpc3 protein in skeletal muscle and osteoblastic cells
Provedor de dados: 48
Autores: SANTILLÁN,G; KATZ,S; BUITRAGO,C; BOLAND,R.
This work describes the involvement of TRPC proteins in capacitative calcium entry (CCE) induced by 1a,25-dihydroxy-vitamin-D3 [1a,25(OH)2D3] in chick skeletal muscle and in rat osteoblast-like cells (ROS 17/2.8) and the role of the vitamin D receptor (VDR) in this non-genomic rapid response mediated by the hormone. We propose that an endogenous TRPC3 protein mediates 1a,25(OH)2D3 modulation of CCE in these cells, which seems to implicate VDR-TRPC3 association and the participation of an INAD-like scaffold protein.
Tipo: Journal article
Palavras-chave: CCE TRPC muscle cells osteoblasts 1a; 25(OH)2D3 VDR INAD.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400021

COLABORACION EN INVESTIGACION ENTRE CHILE Y LA UNION EUROPEA
Provedor de dados: 48
Autores: Jerez,Carlos A.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300001

Omega-3 Fatty Acids and Antioxidants in Edible Wild Plants
Provedor de dados: 48
Autores: SIMOPOULOS,ARTEMIS P.
Human beings evolved on a diet that was balanced in the omega-6 and omega-3 polyunsaturated fatty acids (PUFA), and was high in antioxidants. Edible wild plants provide alpha-linolenic acid (ALA) and higher amounts of vitamin E and vitamin C than cultivated plants. In addition to the antioxidant vitamins, edible wild plants are rich in phenols and other compounds that increase their antioxidant capacity. It is therefore important to systematically analyze the total antioxidant capacity of wild plants and promote their commercialization in both developed and developing countries. The diets of Western countries have contained increasingly larger amounts of linoleic acid (LA), which has been promoted for its cholesterol-lowering effect. It is now recognized...
Tipo: Journal article
Palavras-chave: Alpha-linolenic acid; Antioxidants; Chronic diseases; Edible wild plants; Evolutionary aspects of diet; Omega-3 fatty acids.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200013

Calcium signal compartmentalization
Provedor de dados: 48
Autores: PETERSEN,OLE H.
Cytosolic calcium signals are produced by suddenly increasing the concentration of free calcium ions (Ca2+). This can occur by opening channels permeable to Ca2+ either in the surface cell membrane or in the membranes of intracellular organelles containing high Ca2+ concentrations. Ca2+ signals can control several different processes, even in the same cell. In pancreatic acinar cells, for example, Ca2+ signals do not only control the normal secretion of digestive enzymes, but can also activate autodigestion and programmed cell death. Recent technical advances have shown that different patterns of Ca2+ signals can be created, in space and time, which allow specific cellular responses to be elicited. The mechanisms responsible for Ca2+ signal...
Tipo: Journal article
Palavras-chave: Calcium signals Ca2+ release channels Ca2+ pumps endoplasmic reticulum mitochondria pancreatic acinar cell pancreatitis; Secretion apoptosis.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200008

Microdesiccates produced from normal human tears display four distinctive morphological components
Provedor de dados: 48
Autores: López Solís,Remigio; Traipe Castro,Leonidas; Salinas Toro,Daniela; Srur,Miguel; Toledo Araya,Héctor.
Desiccation of human tears on glass surfaces results in fern-like crystalloids. This phenomenon has been associated with tear normality (Tear Ferning Test, TFT) and is used as a diagnostic aid to evaluate patients with Dry-Eye disease. However, TFT is focused on the assessment of only a minor fraction of desiccated tear samples and considers only the relative abundance and density of fern-like crystalloids. The aim of this study was to characterize morphologically entire desiccated micro volumes of tears from healthy donors. Tear samples were collected from 23 healthy young adult volunteers. Tear aliquots (1-3 &amp;#956;L) were allowed to dry on glass surfaces under ambient conditions of temperature (15-25°C) and relative humidity (40-45%). Dry samples...
Tipo: Journal article
Palavras-chave: Tear; Tear ferning test; Morphometry; Dark-field microscopy.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300012

Using mesenchymal stem cells as a therapy for bone regeneration and repairing
Provedor de dados: 48
Autores: Shao,Jin; Zhang,Weiwei; Yang,Tieyi.
Bone is a unique tissue which could regenerate completely after injury rather than heal itself with a scar. Compared with other tissues the difference is that, during bone repairing and regeneration, after the inflammatory phase the mesenchymal stem cells (MSCs) are recruited to the injury site and differentiate into either chondroblasts or osteoblasts precursors, leading to bone repairing and regeneration. Besides these two precursors, the MSCs can also differentiate into adipocyte precursors, skeletal muscle precursors and some other mesodermal cells. With this multiline-age potentiality, the MSCs are probably used to cure bone injury and other woundings in the near future. Here we will introduce the recent developments in understanding the mechanism of...
Tipo: Journal article
Palavras-chave: Mesenchymal stem cells; Bone regeneration; Bone repairing; Tissue engineering.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100062

Angiogenic potential of the cerebrospinal fluid (CSF) of patients with high-grade gliomas measured with the chick embryo chorioallantoic membrane assay (CAM)
Provedor de dados: 48
Autores: Sinning,Mariana; Letelier,René; Rosas,Carlos; Fuenzalida,Marcela; Lemus,David.
High-grade gliomas are highly vascularized tumors. Neo-angiogenesis plays a key role in tumor growth and resistance to therapy. A cerebrospinal fluid (CSF) sample could be a useful way to obtain pro-angiogenic predictive or prognostic markers at different stages of the disease. As a first step we looked for pro-angiogenic activity in the CSF of patients with high-grade gliomas. We performed the chicken embryo chorio-allantoic membrane (CAM) assay to study the angiogenic potential of the cerebrospinal fluid (CSF), obtained either by lumbar puncture (LP) or craniotomy from six patients with high-grade brain tumors (three glioblastoma (WHO grade IV), one anaplastic oligodendroglioma (WHO grade III), two anaplastic ganglioglioma (WHO grade III)), and four...
Tipo: Journal article
Palavras-chave: Angiogenesis; Cerebrospinal fluid; Glioblastoma; High-grade glioma.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200005

Oligodendrocytes damage in Alzheimer's disease: Beta amyloid toxicity and inflammation
Provedor de dados: 48
Autores: ROTH,ALEJANDRO D; RAMÍREZ,GIGLIOLA; ALARCÓN,RODRIGO; VON BERNHARDI,ROMMY.
Research on Alzheimer's disease (AD) focuses mainly on neuronal death and synaptic impairment induced by &amp;#946;-Amyloid peptide (A&amp;#946;), events at least partially mediated by astrocyte and microglia activation. However, substantial white matter damage and its consequences on brain function warrant the study of oligodendrocytes participation in the pathogenesis and progression of AD. Here, we analyze reports on oligodendrocytes' compromise in AD and discuss some experimental data indicative of A&amp;#946; toxicity in culture. We observed that 1 &amp;#956;M of fibrilogenic A&amp;#946; peptide damages oligodendrocytes in vitro; while pro-inflammatory molecules (1 &amp;#956;g/ml LPS + 1 ng/ml IFN&amp;#947;) or the presence of astrocytes reduced the...
Tipo: Journal article
Palavras-chave: Cell death; Glial cells; Pro-inflammatory cytokines.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400011

Loop-mediated isothermal amplification assays for screening of bacterial integrons
Provedor de dados: 48
Autores: Yu,Guangchao; Chen,Lei; Lin,Chii-wann; Li,Bing; Cui,Hemiao; Chen,Siyi; Miao,Jian; Bian,Huawei; Chen,Dingqiang; Deng,Yang.
BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for integrons detection. RESULTS: In this study, three loop-mediated isothermal amplification (LAMP) assays targeting on class 1, 2 and 3 integrons were implemented and evaluated. Optimization of these detection assays were performed, including studing on the reaction temperature, volume, time, sensitivity and specificity (both primers and targets). Application of the established LAMP assays were further verified on a total of 1082...
Tipo: Journal article
Palavras-chave: Loop-mediated isothermal amplification (LAMP); Integron screening; Bacterial integrons; Class 1 integron; Class 2 integron; Class 3 integron.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100076

Chemical and electric transmission in the carotid body chemoreceptor complex
Provedor de dados: 48
Autores: EYZAGUIRRE,CARLOS.
Carotid body chemoreceptors are complex secondary receptors. There are chemical and electric connections between glomus cells (GC/GC) and between glomus cells and carotid nerve endings (GC/NE). Chemical secretion of glomus cells is accompanied by GC/GC uncoupling. Chemical GC/NE transmission is facilitated by concomitant electric coupling. Chronic hypoxia reduces GC/GC coupling but increases G/NE coupling. Therefore, carotid body chemoreceptors use chemical and electric transmission mechanisms to trigger and change the sensory discharge in the carotid nerve.
Tipo: Journal article
Palavras-chave: Carotid body; Chemosensory activity; Glomus cells.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400005

Donepezil reverses buprenorphine-induced central respiratory depression in anesthetized rabbits
Provedor de dados: 48
Autores: SAKURABA,SHIGEKI; TSUJITA,MIKI; ARISAKA,HIROFUMI; TAKEDA,JUNZO; YOSHIDA,KAZUICHI; KUWANA,SHUN-ICHI.
Buprenorphine is a mixed opioid receptor agonist-antagonist used in acute and chronic pain management. Although this agent's analgesic effect increases in a dose-dependent manner, buprenorphine-induced respiratory depression shows a marked ceiling effect at higher doses, which is considered to be an indicator of safety. Nevertheless, cases of overdose mortality or severe respiratory depression associated with buprenorphine use have been reported. Naloxone can reverse buprenorphine-induced respiratory depression, but is slow-acting and unstable, meaning that new drug candidates able to specifically antagonize buprenorphine-induced respiratory depression are needed in order to enable maximal analgesic effect without respiratory depression. Acetylcholine is...
Tipo: Journal article
Palavras-chave: Buprenorphine; Donepezil; Rabbit; Respiratory depression; Cholinesterase inhibitor.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400008

Interaction of Olive Oil Phenol Antioxidant Components with Low-density Lipoprotein
Provedor de dados: 48
Autores: LAMUELA-RAVENTÓS,ROSA M; GIMENO,EVA; FITÓ,MONTSE; CASTELLOTE,ANA-ISABEL; COVAS,MARIBEL; DE LA TORRE-BORONAT,M. CARMEN; LÓPEZ-SABATER,M. CARMEN.
Phenolic compounds have shown to inhibit LDL oxidation in vitro and ex vivo; however, they are hydrosoluble compounds while LDL is a lipoprotein. Analysis of phenolic compounds in LDLs by HPLC is necessary to demonstrate their binding capacity to lipoproteins. We developed and validated a solid phase extraction method (SPE) that allowed us the purification of LDL samples and their analysis by HPLC. This methodology allowed us to demonstrate the in vitro binding capacity of tyrosol, one of the main phenolic compounds in olive oil, to LDL. In the intervention dietary study with volunteers, food rich in phenolic compounds affected LDL composition. Changes in LDL phenolics composition are not observed after the short-term ingestion of food rich in phenolic...
Tipo: Journal article
Palavras-chave: Antioxidant; LDL; Olive oil; Oxidation; Phenol; Tyrosol.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200011

G2 repair and chromosomal damage in lymphocytes from workers occupationally exposed to low-level ionizing radiation
Provedor de dados: 48
Autores: PINCHEIRA,J; LÓPEZ,I; SANHUEZA,S; FERRUZ,P; NAVRRETE,MH; SANTOS,MJ; LÓPEZ-SÁEZ,JF.
The effect of the G2 repair of chromosomal damage in lymphocytes from workers exposed to low levels of X- or g-rays was evaluated. Samples of peripheral blood were collected from 15 radiation workers, 20 subjects working in radiodiagnostics, and 30 healthy control donors.  Chromosomal aberrations (CA) were evaluated by scoring the presence of chromatid and isochromatid breaks, dicentric and ring chromosomes in lymphocytes with/without 5mM caffeine plus 3mM-aminobenzamide (3-AB) treatment during G2.  Our results showed that the mean value of basal aberrations in lymphocytes from exposed workers was higher than in control cells (p&amp;lt; 0.001). The chromosomal damage in G2, detected with caffeine plus 3-AB treatment was higher than the basal damage...
Tipo: Journal article
Palavras-chave: Chromosome aberrations ionizing radiation; G2 repair; Human lymphocytes; Occupational exposure.
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400009

The physiological role of the unfolded protein response in plants
Provedor de dados: 48
Autores: Moreno,Adrian A; Orellana,Ariel.
Unfolded protein response (UPR) is a signaling mechanism activated by misfolded protein accumulation in the endoplasmic reticulum. It is a widespread process that has been described in organisms ranging from yeasts to mammals. In recent years, our understanding of UPR signaling pathway in plants has advanced. Two transcription factors from Arabidopsis thaliana have been reported to function as the sensor/ transducer of this response (AtbZIP60 and AtbZIP28). They seem to be involved in both heat and biotic stress. Furthermore, overexpression of one of them (AtbZIP60) produces plants with a higher tolerance for salt stress, suggesting that this transcription factor may play a role in abiotic stress. Furthermore, some data suggest that crosstalk between genes...
Tipo: Journal article
Palavras-chave: Endoplasmic reticulum; Plant; Signaling; Unfolded protein response.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100010

Alcohol and Mortality from All Causes
Provedor de dados: 48
Autores: RENAUD,SERGE; LANZMANN-PETITHORY,DOMINIQUE; GUEGUEN,RENÉ; CONARD,PASCALE.
A large number of prospective studies have observed an inverse relationship between a moderate intake of alcohol and coronary heart disease morbidity and mortality. Concerning death from all-causes, results are not unanimous. Alcohol intake was associated with a protection of all-cause mortality in England and USA physicians and the large study of the American Cancer Society. None of these studies separated the effects of different alcoholic beverages. In our prospective studies in France on 35 000 middle-aged men, we observed that only wine at moderate intake, was associated with a protective effect on all-cause mortality. The reason was that in addition to the known effect on cardiovascular diseases, a very moderate intake of wine, protected also from...
Tipo: Journal article
Palavras-chave: Alcohol; Mortality; Wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200002

Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
Provedor de dados: 48
Autores: Ellahueñe,Manuel F; Pérez-Alzola,L Patricia; Olmedo,M Isabel.
Although alcohol is known to be a carcinogen for humans, ethanol-genotoxicity studies are incomplete. Ethanol seems not to be a bacterial mutagen, but the results are conflicting in rodent assays. We investigate the genotoxicity in the bone marrow micronucleus (MN) test and in the dominant lethal mutation (DLM) assay using two long-term ethanol exposure protocols. In the MN test, mice consumed three doses (5, 10 and 15% v/v) for 32 weeks. MN induction was compared to two control groups of 5- and 38-week-old mice (the ages of the treated mice when the treatment was initiated and when they were killed, respectively). For the three groups treated with ethanol there was no significant increase in MN induction as compared to the first control group, but...
Tipo: Journal article
Palavras-chave: Dominant lethal mutation; Ethanol; Genotoxicity; Micronucleus test.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100004

L REUNIÓN ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CHILE: CONFERENCIAS
Provedor de dados: 48
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000300002

Posttranslational protein S-palmitoylation and the compartmentalization of signaling molecules in neurons
Provedor de dados: 48
Autores: PATTERSON,SEAN I.
Protein domains play a fundamental role in the spatial and temporal organization of intracellular signaling systems. While protein phosphorylation has long been known to modify the interactions that underlie this organization, the dynamic cycling of lipids should now be included amongst the posttranslational processes determining specificity in signal transduction. The characteristics of this process are reminiscent of the properties of protein and lipid phosphorylation in determining compartmentalization through SH2 or PH domains. Recent studies have confirmed the functional importance of protein S-palmitoylation in the compartmentalization of signaling molecules that support normal physiological function in cell division and apoptosis, and synaptic...
Tipo: Journal article
Palavras-chave: S-palmitoylation; Synapse; Growth cone; Regeneration; Phosphorylation.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200005

A cell junction pathology of neural stem cells leads to abnormal neurogenesis and hydrocephalus
Provedor de dados: 48
Autores: Rodríguez,Esteban M; Guerra,María M; Vío,Karin; González,César; Ortloff,Alexander; Bátiz,Luis F; Rodríguez,Sara; Jara,María C; Muñoz,Rosa I; Ortega,Eduardo; Jaque,Jaime; Guerra,Francisco; Sival,Deborah A; Dunnen,Wilfred F. A. den; Jiménez,Antonio J; Domínguez-Pinos,María D; Pérez-Fígares,José M; McAllister,James P; Johanson,Conrad.
Most cells of the developing mammalian brain derive from the ventricular (VZ) and the subventricular (SVZ) zones. The VZ is formed by the multipotent radial glia/neural stem cells (NSCs) while the SVZ harbors the rapidly proliferative neural precursor cells (NPCs). Evidence from human and animal models indicates that the common history of hydrocephalus and brain maldevelopment starts early in embryonic life with disruption of the VZ and SVZ. We propose that a "cell junction pathology" involving adherent and gap junctions is a final common outcome of a wide range of gene mutations resulting in proteins abnormally expressed by the VZ cells undergoing disruption. Disruption of the VZ during fetal development implies the loss of NSCs whereas VZ disruption...
Tipo: Journal article
Palavras-chave: Hydrocephalus; Abnormal neurogenesis; Neural stem cells; Stem cell transplantation.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300005

Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae
Provedor de dados: 48
Autores: REYES,PAZ A; VARGAS,MACARENA F; GARCÍA,KATHERINE P; RUBILAR,PAULINA S; NAVARRETE,PATRICIA A; FUENTES,PAMELA M; RÍOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H; RUBIO,VÍCTOR H; HECKELS,JOHN; CHRISTODOULIDES,MYRON; CÁRDENAS,HUGO; VELASQUEZ,LUIS A.
Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-alpha dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of...
Tipo: Journal article
Palavras-chave: Apoptosis; Fallopian tube; Neisseria gonorrhoeae; Salpingitis; TNF-alpha.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400006

Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-&amp;#945; and IL-1&amp;#946; production in THP-1 cells
Provedor de dados: 48
Autores: Shu,Jun; He,Xiaojuan; Zhang,Lan; Li,Hong; Wang,Ping; Huang,Xiaojie.
BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-&amp;#945; and IL-1&amp;#946; secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the...
Tipo: Journal article
Palavras-chave: Human amnion mesenchymal cells; THP-1 cells; TNF-ZZZZZZZ; IL-1XXXXXXXX; Immunosuppression.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069

Cardiovascular and ventilatory acclimatization induced by chronic intermittent hypoxia: A role for the carotid body in the pathophysiology of sleep apnea
Provedor de dados: 48
Autores: ITURRIAGA,RODRIGO; REY,SERGIO; DEL RÍO,RODRIGO.
Patients with obstructive sleep apnea (OSA) show augmented ventilatory, sympathetic and cardiovascular responses to hypoxia. The facilitatory effect of chronic intermittent hypoxia (CIH) on the hypoxic ventilatory response has been attributed to a potentiation of the carotid body (CB) chemosensory response to hypoxia. However, it is a matter of debate whether the effects induced by CIH on ventilatory responses to hypoxia are due to an enhanced CB activity. Recently, we studied the effects of short cyclic hypoxic episodes on cat cardiorespiratory reflexes, heart rate variability, and CB chemosensory activity. Cats were exposed to cyclic hypoxic episodes repeated during 8 hours for 4 days. Our results showed that CIH selectively enhanced ventilatory and...
Tipo: Journal article
Palavras-chave: Ventilatory acclimatization; Carotid body; Chemosensory activity; Intermittent hypoxia; Obstructive sleep apnea.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400004

Biomarkers for screening of lung cancer and pre-neoplastic lesions in a high risk Chilean population
Provedor de dados: 48
Autores: Adonis,Marta I; Díaz,Jose; Miranda,Veronica R; Chahuan,Marco; Zambrano,Alcides; Benitez,Hugo C; Campos,Monica; Avaria,Pablo; Urzúa,Ulises; Marín,Pedro; Gohurdett,Mariela; Cisterna,Yasna; Gil,Lionel.
BACKGROUND: The mortality of lung cancer (LC), increases each year in the world, in spite of any advances, in development of new drugs to advance stages of LC. The high incidence of LC has been associated with smoking habit, genetic diversity and environmental pollution. Antofagasta region has been reported to have the highest LC mortality rate in Chile and its inhabitants were exposed to arsenic in their drinking water in concentrations as high as 870 &amp;#956;g/L. Non-invasive techniques such as biomarkers (Automatic Quantitative Cytometry: AQC and DR70) and Auto Fluorescence Bronchoscopy (AFB) might be potentially useful as a supplementary diagnostic approach and early detection. Early detection is one of the most important factors to intervene and...
Tipo: Journal article
Palavras-chave: Early detection; Biomarkers; Lung cancer.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100056

Protective effect of Hypericum calabricum Sprengel on oxidative damage and its inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages
Provedor de dados: 48
Autores: Statti,Giancarlo A; Conforti,Filomena; Menichini,Federica; Marrelli,Mariangela; Gangale,Carmen; Tundis,Rosa; Loizzo,Monica R; Bonesi,Marco; Menichini,Francesco.
The present study shows for the first time the phenolic composition and the in vitro properties (antioxidant and inhibition of nitric oxide production) of Hypericum calabricum Sprengel collected in Italy. The content of hypericins (hypericin and pseudohypericin), hyperforin, flavonoids (rutin, hyperoside, isoquercetrin, quercitrin, quercetin and biapigenin) and chlorogenic acid of H. calabricum, have been determined. The ethyl acetate fraction from the aerial parts of H. calabricum exhibited activity against the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 value of 1.6 jig/ml. The test for inhibition of nitric oxide (NO) production was performed using the murine monocytic macrophage cell line RAW 264.7. The ethyl acetate fraction had significant...
Tipo: Journal article
Palavras-chave: Antiradical activity; Hypericaceae; Hypericum calabricum Sprengel; Phenolic compounds.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300001

Histocompatibility and Immunogenetics in Cord Blood Transplantation
Provedor de dados: 48
Autores: Rubinstein,Pablo; Hillyer,Christopher.
This review of the immunogenetics of cord blood transplantation attempts to highlight the connections between classical studies and conclusions of the tissue transplantation field as a scholarly endeavor, exemplified by the work of Professor Hoecker, with the motivations and some recent and key results of clinical cord blood transplantation. The authors review the evolution of understanding of transplantation biology and find that the results of the application of cord blood stem cells to Transplantation Medicine are consistent with the careful experiments of the pioneers in the field, from the results of tumor and normal tissue transplants, histocompatibility immunogenetics, to cell and molecular biology. Recent results of the National Cord Blood Program...
Tipo: Journal article
Palavras-chave: Umbilical Cord Blood; Hematopoietic Transplantation; Histocompatibility Genes; HLA matching.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300011

De novo transcriptome assembly, functional annotation and differential gene expression analysis of juvenile and adult E. fetida, a model oligochaete used in ecotoxicological studies
Provedor de dados: 48
Autores: Thunders,Michelle; Cavanagh,Jo; Li,Yinsheng.
Abstract  Background  Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression.  Results  This paper reports on the de novo transcriptome assembly of E. fetida using Trinity, a freely available software tool. Trinotate was used to carry out functional annotation of the Trinity generated transcriptome file and the transdecoder generated peptide sequence file along with BLASTX, BLASTP and HMMER searches and were loaded into a Sqlite3 database. To identify differentially expressed...
Tipo: Journal article
Palavras-chave: Earthworm; Trinity; Transcriptome; E. fetida; Ecotoxicology; RNA; Sequence; Gene expression.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100401

Sarcophaga (Liosarcophaga) dux (Diptera: Sarcophagidae): A flesh fly species of medical importance
Provedor de dados: 48
Autores: Sukontason,Kabkaew L; Sanit,Sangob; Klong-klaew,Tunwadee; Tomberlin,Jeffery K; Sukontason,Kom.
BACKGROUND: Although tropical climate of Thailand is suitably endowed with biodiversity of insects, flies of medical importance is not well investigated. Using information from literature search, fly survey approach and specialist's experience, we review database of Sarcophaga (Liosarcophaga) dux Thomson (Diptera: Sarcophagidae), one of the priorities flesh fly species of medical importance in Thailand. RESULTS: This review deals with morphology, bionomics and medical involvement. Important morphological characteristics of egg, larva, puparia and adult were highlighted with illustration and/or micrographs. Search pertaining to molecular analysis used for fly identification and developmental rate of larvae were included. Medical involvement of larvae was...
Tipo: Journal article
Palavras-chave: Sarcophaga dux; Review literature; Thailand; Forensic entomology; Myiasis; Morphology; Adult; Immature stages.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100014

Erratum
Provedor de dados: 48
Tipo: Journal article
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200014

Correlations of recognition memory performance with expression and methylation of brain-derived neurotrophic factor in rats
Provedor de dados: 48
Autores: Muñoz,Pablo C; Aspé,Mauricio A; Contreras,Luis S; Palacios,Adrián G.
Object recognition memory allows discrimination between novel and familiar objects. This kind of memory consists of two components: recollection, which depends on the hippocampus, and familiarity, which depends on the perirhinal cortex (Pcx). The importance of brain-derived neurotrophic factor (BDNF) for recognition memory has already been recognized. Recent evidence suggests that DNA methylation regulates the expression of BDNF and memory. Behavioral and molecular approaches were used to understand the potential contribution of DNA methylation to recognition memory. To that end, rats were tested for their ability to distinguish novel from familiar objects by using a spontaneous object recognition task. Furthermore, the level of DNA methylation was...
Tipo: Journal article
Palavras-chave: DNA methylation; Recognition memory; Synaptic plasticity; Hippocampal; Perirhinal cortex; BDNF.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200013

miR-22 suppresses tumorigenesis and improves radiosensitivity of breast cancer cells by targeting Sirt1
Provedor de dados: 48
Autores: Zhang,Xia; Li,Yuehua; Wang,Dan; Wei,Xiaoer.
Abstract  Background  miR-22 has been shown to be frequently downregulated and act as a tumor suppressor in multiple cancers including breast cancers. However, the role of miR-22 in regulating the radioresistance of breast cancer cells, as well as its underlying mechanism is still not well understood.  Methods  The expressions of miR-22 and sirt1 at mRNA and protein levels were examined by qRT-PCR and Western Blot. The effects of miR-22 overexpression and sirt1 knockdown on cell viability, apoptosis, radiosensitivity, &amp;#947;-H2AX foci formation were evaluated by CCK-8 assay, flow cytometry, colony formation assay, and &amp;#947;-H2AX foci formation assay, respectively. Luciferase reporter assay and qRT-PCR analysis were performed to confirm the...
Tipo: Journal article
Palavras-chave: MiR-22; Tumorigenesis; Radiosensitivity; Breast cancer; Sirt1.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100220

Candidate genes and pathogenesis investigation for sepsis-related acute respiratory distress syndrome based on gene expression profile
Provedor de dados: 48
Autores: Wang,Min; Yan,Jingjun; He,Xingxing; Zhong,Qiang; Zhan,Chengye; Li,Shusheng.
BACKGROUND: Acute respiratory distress syndrome (ARDS) is a potentially devastating form of acute inflammatory lung injury as well as a major cause of acute respiratory failure. Although researchers have made significant progresses in elucidating the pathophysiology of this complex syndrome over the years, the absence of a universal detail disease mechanism up until now has led to a series of practical problems for a definitive treatment. This study aimed to predict some genes or pathways associated with sepsis-related ARDS based on a public microarray dataset and to further explore the molecular mechanism of ARDS RESULTS: A total of 122 up-regulated DEGs and 91 down-regulated differentially expressed genes (DEGs) were obtained. The up- and down-regulated...
Tipo: Journal article
Palavras-chave: Acute respiratory distress syndrome; Sepsis; Differentially expressed mRNAs; Functional enrichment analysis; Pathway analysis; Transcription factors.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100025

In vitro assessment of a computer-designed potential anticancer agent in cervical cancer cells
Provedor de dados: 48
Autores: Visagie,Michelle Helen; Jaiswal,Seema Rummurat; Joubert,Anna Margaretha.
BACKGROUND: Computer-based technology is becoming increasingly essential in biological research where drug discovery programs start with the identification of suitable drug targets. 2-Methoxyestradiol (2ME2) is a 17ß-estradiol metabolite that induces apoptosis in various cancer cell lines including cervical cancer, breast cancer and multiple myeloma. Owing to 2ME2's poor in vivo bioavailability, our laboratory in silico-designed and subsequently synthesized a novel 2ME2 analogue, 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10),15-tetraen-17-ol (ESE-15-ol), using receptor- and ligand molecular modeling. In this study, the biological effects of ESE-15-ol (180 nM) and its parent molecule, 2ME2 (1 &amp;#956;M), were assessed on morphology and apoptosis induction in...
Tipo: Journal article
Palavras-chave: Morphology; Apoptosis; Cervical cancer; ESE-15-ol.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100043

Relevance of a neutral cue in a two-choice detection task in the rat
Provedor de dados: 48
Autores: HAMAME,CARLOS M; DELANO,PAUL H; ROBLES,LUIS.
Relevance of a neutral cue for performance in a two-choice visuospatial detection task was examined. Nine rats were trained, 5 with short intertrial interval (ITI) and 4 with long ITI, to detect a target (lateral lights) presented after a neutral-cue (central light). The removal of the neutral-cue decreased accuracy and increased response latencies and omissions. These results demonstrate that a neutral-cue, preceding the target, is relevant for the performance, suggesting that rats are highly expectant during the neutral-cue and reallocate attentional resources during ITI. Furthermore, latencies were higher, omissions were lower and the fall of accuracy was greater for rats with long than with short ITI, which could indicate that the neutral-cue was more...
Tipo: Journal article
Palavras-chave: Attention; Expectancy; Neutral cue; Attentional task; Rat.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200008

Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
Provedor de dados: 48
Autores: PETACCI,FERNANDO; FREITAS,SILVIA S; BRUNETTI,IGUATEMY L; KHALIL,NAJEH M.
Astilbin (5,7,3’,4’-tetrahydroxy-2,3-dihydroflavonol-3-ß-o-rhamnoside), a flavonoid with a large range of biological activities, was isolated from Dimorphandra mollis, a shrub common to the Brazilian Cerrado. The purpose of this study is to verify the effects of astilbin on myeloperoxidase (MPO) and horseradish peroxidase (HRP), and its antioxidant activity against hypochlorous acid (HOCl) and total antioxidant activity (TAC) by the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS•+). Astilbin inhibited MPO and HRP activities in a concentration-dependent relationship and effectively scavenged HOCl. The TAC by ABTS•+ of astilbin (IC50 ~ 20 mM) was higher than that of uric acid, which was used as a positive control. These data...
Tipo: Journal article
Palavras-chave: Astilbin; Myeloperoxidase; Horseradish peroxidase; Reactive Oxygen Species; Hypochlorous acid.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100008

Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells
Provedor de dados: 48
Autores: Xu,Huiyun; Zhang,Jian; Wu,Jiawei; Guan,Ying; Weng,Yuanyuan; Shang,Peng.
Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is little information about the response of integrin-associated molecules using both osteoblasts and osteocytes. In this study, we investigated the changes in response to 2 hours of oscillatory fluid flow stress in the MLO-Y4 osteocyte-like cell line and the MC3T3-E1 osteoblast-like cell line. MLO-Y4 cells exhibited a significant increase in the expression of integrin-associated molecules, including OPN, CD44, vinculin and integrin avp3. However,...
Tipo: Journal article
Palavras-chave: Cytoskeleton; Fluid flow stress; Integrin-associated molecules; MC3T3-E1 osteoblast-like cell line; MLO-Y4 osteocyte-like cell line.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200008

Biophilosophical and epistemological problems in the study of living beings: Reflections on the views of Humberto Maturana
Provedor de dados: 48
Autores: SERANI-MERLO,ALEJANDRO.
Theories on the nature of living beings have been present in our culture since the beginnings of science and philosophy in ancient Greece. The two major theoretical approaches to living beings, philosophical mechanism and Aristotelian realism, appear today with renewed force in almost every confrontation concerning the theoretical considerations of life. In recent times a strong and prolific school of thought has risen, headed by the Chilean neurobiologist Humberto Maturana. This author and his school have developed a complex and articulated theoretical system beginning with a theory of living beings and a `biology of cognition,' and extending to ethical, political, and even metaphysical considerations. This work is one of the first efforts to perform a...
Tipo: Journal article
Palavras-chave: Biophilosophy; Determinism; Philosophical mechanism; Finality.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300005

Antioxidant activity of extract from Polygonum cuspidatum
Provedor de dados: 48
Autores: HSU,CHIN-YUAN; CHAN,YU-PEI; CHANG,JELI.
Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum cuspidatum Sieb. et Zuce has antioxidant activity is unknown. In this study, dried roots of Polygonum cuspidatum were extracted by ethanol and the extract was lyophilized. Free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays were employed to study antioxidant activities. The results indicate that the IC50 value oí Polygonum cuspidatum extract is 110 µg/ml in free radical scavenging assays, 3.2 µg/ml in superoxide radical scavenging...
Tipo: Journal article
Palavras-chave: Antioxidant activity; Free radicals; Phenolics; Lipid peroxidation; DNA damage Polygonum cuspidatum.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100002

In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
Provedor de dados: 48
Autores: RIOS,MARIANA; VENEGAS,ALEJANDRO; CROXATTO,HORACIO B.
Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (&lt;A HREF="#rios97"&gt;Ríos et al., 1997&lt;/A&gt;). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal...
Tipo: Journal article
Palavras-chave: SS-galactosidase; Gene transfer; Oviduct.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007

Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
Provedor de dados: 48
Autores: Traipe-Salas,Felipe; Traipe-Castro,Leonidas; Salinas-Toro,Daniela; López,Daniela; Valenzuela,Felipe; Cartes,Christian; Toledo-Araya,Héctor; Pérez,Claudio; Solís,Remigio López.
BACKGROUND: Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamper comprehensive microscopic examination of tear microdesiccates. Optimal observation conditions of entire tear microdesiccates are now investigated. One-&amp;#956;! aliquots of tear collected from individual healthy eyes were dried at ambient conditions on microscope slides. Tear microdesiccates were examined by combining low-magnification objective lenses with transmitted-light microscopy (brightfield, phase contrasts Ph1,2,3 and...
Tipo: Journal article
Palavras-chave: Tear; Tear ferning test; Transmitted-light microscopy; Tear microdesiccate; Dry eye.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100028

In vitro approach to the chemical drive of breathing
Provedor de dados: 48
Autores: EUGENIN,JAIME; LLONA,ISABEL; INFANTE,CLAUDIA D; AMPUERO,ESTIBALIZ.
Since its introduction two decades ago, the isolated brain stem-spinal cord preparation of neonatal rodents has been the preferred method used to reveal the mystery underlying the genesis of the respiratory rhythm. Little research using this in vitro approach has focused on the study of the central respiratory chemosensitivity. Some unexpected findings obtained with the brain stem-spinal cord preparation have added new questions that challenge our previous theoretic framework. Some of these findings are addressed here.
Tipo: Journal article
Palavras-chave: Central chemoreceptors; Control of breathing; Ventral medulla; Brain stem.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200016

Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary
Provedor de dados: 48
Autores: Li,Pengfei; Yu,Xuejing; Xie,Jianshan; Yao,Xiaolei; Liu,Wenzhong; Yao,Jianbo; Zhu,Zhiwei; Lyu,Lihua.
Abstract  Background: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role...
Tipo: Journal article
Palavras-chave: Hen; Follicles; CART; Theca cell; Granulosa cell.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100211

XLIII REUNION ANUAL DE LA SOCIEDAD DE BIOLOGIA DE CHILE
Provedor de dados: 48
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300009

Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
Provedor de dados: 48
Autores: Almeida,Fabiana Greyce Oliveira; Aquino,Priscila Ferreira de; Souza,Afonso Duarte Leäo de; Souza,Antonia Queiroz Lima de; Vinhote,Sonia do Carmo; Mac-Cormick,Thais Messias; Silva,Marcelo Soares da Mota; Chalub,Sidney Raimundo Silva; Fischer,Juliana de Saldanha da Gama; Carvalho,Paulo Costa; Carvalho,Maria da Gloria da Costa.
BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2...
Tipo: Journal article
Palavras-chave: Methylation; Colorectal cancer; Amazon; Epigenetic.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050

Editorial
Provedor de dados: 48
Autores: Inestrosa C.,Nibaldo.
Tipo: Journal article
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300001

Los ex -Presidentes de la Sociedad de Biología se reunieron en Pucón
Provedor de dados: 48
Tipo: Journal article
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400003

Essential oils from two Eucalyptus from Tunisia and their insecticidal action on Orgyia trigotephras (Lepidotera, Lymantriidae)
Provedor de dados: 48
Autores: Slimane,Badreddine Ben; Ezzine,Olfa; Dhahri,Samir; Jamaa,Mohamed Lahbib Ben.
BACKGROUND: Essential oils extracted from aromatic and medicinal plants have many biological properties and are therefore an alternative to the use of synthetic products. The chemical composition of essential oils from two medicinal plants (Eucalyptus globulus and E. lehmannii) was determined and, their insecticidal effects on the third and fourth larval stages of Orgyia trigotephras were assessed. RESULTS: Larvae were collected from Jebel Abderrahmane (North-East of Tunisia), conserved in groups of 50/box (21 × 10 × 10 cm) at a temperature of 25°C. Larvae were tested for larvicidal activities of essential oils. Each oil was diluted in ethanol (96%) to prepare 3 test solutions (S1 = 0.05%, S2 = 0.10% and S3 = 0.50%). Essential oils were used for contact,...
Tipo: Journal article
Palavras-chave: Eucalyptus; Orgyia trigotephras; Essential oils; Insecticides; Insect control.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100029

Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)
Provedor de dados: 48
Autores: LODATO,PATRICIA; ALCAÍNO,JENNIFER; BARAHONA,SALVADOR; RETAMALES,PATRICIO; JIMÉNEZ,ANTONIO; CIFUENTES,VÍCTOR.
The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type strain (UCD 67-385) and two overproducer deregulated strains (atxS1 and atxS2). At 3 day cultures, the wild-type strain contained higher transcript levels from the crtE and crtYB genes on minimal medium than on rich medium. Similarly, carotenoid production was higher on minimal medium than on rich medium. However, carotenoid production in the atxS1 and atxS2 strains was...
Tipo: Journal article
Palavras-chave: Carotenoid biosynthesis; Overproducer strains; Xanthophyllomyces dendrorhous; Phaffia rhodozyma.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009

New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse
Provedor de dados: 48
Autores: FAROUDI,MUSTAPHA; ZARU,ROSSANA; FAVIER,BENOIT; VALITUTTI,SALVATORE.
Three innovative and complementary morphological approaches were employed to study the T cell/antigen presenting cell (APC) interaction: (i) high resolution three-dimensional confocal microscopy of the T cell-APC contact site; (ii) time lapse video recording in living T cells of [Ca2+]I and changes in distribution of various GFP fusion proteins with TCR/CD3-zetacomplex associated- and other signaling components; (iii) measurement of lateral TCR mobility and that of recruited signaling components using techniques based on fluorescence recovery after photo-bleaching. These approaches were combined with biochemical and functional experiments to investigate two principal issues: (A) Recruitment and the three-dimensional arrangement of receptors and signaling...
Tipo: Journal article
Palavras-chave: T cell antigen receptor; Immunological synapse; T cell antigen receptor; Signal transduction.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200004

EDITORIAL
Provedor de dados: 48
Autores: SANTOS,MANUEL J.
Tipo: Journal article
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000300001

Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous
Provedor de dados: 48
Autores: LODATO,P; ALCAÍNO,J; BARAHONA,S; NIKLITSCHEK,M; CARMONA,M; WOZNIAK,A; BAEZA,M; JIMÉNEZ,A; CIFUENTES,V.
In the yeast Xanthophyllomyces dendrorhous the genes idi, crtE, crtYB, crtl and ast are involved in the biosynthesis of astaxanthin from isopentenyl pyrophosphate. The carotenoid production and the kinetics of mRNA expression of structural genes controlling the carotenogenesis in a wild-type ATCC 24230 and in carotenoid overproducer deregulated atxS2 strains were studied. The biosynthesis of carotenoid was induced at the late exponential growth phase in both strains. However, the cellular carotenoid concentration was four times higher in atxS2 than in the wild-type strain in the exponential growth phase, suggesting that carotenogenesis was deregulated in atxS2 at the beginning of growth. In addition, the maximum expression of the carotenogenesis genes at...
Tipo: Journal article
Palavras-chave: Xanthophyllomyces dendrorhous; Carotenogenesis genes; MRNA expression.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100008

Cholesterol oxidation: Health hazard and the role of antioxidants in prevention
Provedor de dados: 48
Autores: VALENZUELA,ALFONSO; SANHUEZA,JULIO; NIETO,SUSANA.
Cholesterol is a molecule with a double bond in its structure and is therefore susceptible to oxidation leading to the formation of oxysterols. These oxidation products are found in many commonly-consumed foods and are formed during their manufacture and/or processing. Concern about oxysterols consumption arises from the potential cytotoxic, mutagenic, atherogenic, and possibly carcinogenic effects of some oxysterols. Eggs and egg-derived products are the main dietary sources of oxysterols. Thermally-processed milk and milk-derived products are another source of oxysterols in our diet. Foods fried in vegetable/animal oil, such as meats and French-fried potatoes, are major sources of oxysterols in the Western diet. Efforts to prevent or to reduce...
Tipo: Journal article
Palavras-chave: Cholesterol; Cholesterol oxidation; Oxysterols; Synthetic antioxidants; Natural antioxidants.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300002

Letters to Editor / Erratum
Provedor de dados: 48
Autores: Valenzuela,Carlos Y.
Tipo: Journal article
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300015

Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment
Provedor de dados: 48
Autores: ALATZAS,ANASTASIOS; SREBREVA,LJUBA; FOUNDOULI,ATHINA.
Although several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study, we identified linker histone variants by using specific anti-histone Hl antibodies. Each variant's ratio to total Hl in the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them was estimated in order to define possible alterations either during plant cell differentiation or during their dedifferentiation. We also evaluated linker histone variants' ratios in the developmental zones of maize roots treated with auxin in order to examine the...
Tipo: Journal article
Palavras-chave: Callus culture; Linker histone; Plant cell differentiation; Plant hormone; Zea mays.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200010

Biological Research marks its 50th year by joining BioMed Central
Provedor de dados: 48
Autores: Santos,Manuel J.
Tipo: Journal article
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100001

Rapid culture-based detection of living mycobacteria using microchannel electrical impedance spectroscopy (m-EIS)
Provedor de dados: 48
Autores: Kargupta,Roli; Puttaswamy,Sachidevi; Lee,Aiden J.; Butler,Timothy E.; Li,Zhongyu; Chakraborty,Sounak; Sengupta,Shramik.
Abstract  Background: Multiple techniques exist for detecting Mycobacteria, each having its own advantages and drawbacks. Among them, automated culture-based systems like the BACTEC-MGIT&amp;#8482; are popular because they are inexpensive, reliable and highly accurate. However, they have a relatively long "time-to-detection&amp;#8221; (TTD). Hence, a method that retains the reliability and low-cost of the MGIT system, while reducing TTD would be highly desirable.  Methods: Living bacterial cells possess a membrane potential, on account of which they store charge when subjected to an AC-field. This charge storage (bulk capacitance) can be estimated using impedance measurements at multiple frequencies. An increase in the number of living cells during culture...
Tipo: Journal article
Palavras-chave: Mycobacteria; Tuberculosis; Electrical impedance spectroscopy; BD Bactec MGIT 960; M. bovis BCG; M. smegmatis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100214

Neutral space analysis for a Boolean network model of the fission yeast cell cycle network
Provedor de dados: 48
Autores: Ruz,Gonzalo A; Timmermann,Tania; Barrera,Javiera; Goles,Eric.
BACKGROUND: Interactions between genes and their products give rise to complex circuits known as gene regulatory networks (GRN) that enable cells to process information and respond to external stimuli. Several important processes for life, depend of an accurate and context-specific regulation of gene expression, such as the cell cycle, which can be analyzed through its GRN, where deregulation can lead to cancer in animals or a directed regulation could be applied for biotechnological processes using yeast. An approach to study the robustness of GRN is through the neutral space. In this paper, we explore the neutral space of a Schizosaccharomyces pombe (fission yeast) cell cycle network through an evolution strategy to generate a neutral graph, composed of...
Tipo: Journal article
Palavras-chave: Neutral graph; Boolean networks; Evolution strategy; Fission yeast cell cycle; Attractors.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100058

Olfactomedin-like 2 A and &amp;#914; (OLFML2A and OLFML2B) expression profile in primates (human and baboon)
Provedor de dados: 48
Autores: Pérez-Ibave,Diana Cristina; González-Alvarez,Rafael; Martinez-Fierro,Margarita de La Luz; Ruiz-Ayma,Gabriel; Luna-Munoz,Maricela; Martínez-De-Villarreal,Laura Elia; Garza-Rodríguez,María De Lourdes; Reséndez-Pérez,Diana; Mohamed-Noriega,Jibran; Garza-Guajardo,Raquel; Bautista-De-Lucío,Victor Manuel; Mohamed-Noriega,Karim; Barboza-Quintana,Oralia; Arámburo-De-La-Hoz,Carlos; Barrera-Saldana,Hugo Alberto; Rodríguez-Sánchez,Irám Pablo.
Tipo: Journal article
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100044

Propylthiouracil-induced hypothyroidism delays apoptosis during the first wave of spermatogenesis
Provedor de dados: 48
Autores: Silva,Doris; Lizama,Carlos; Tapia,Verónica; Moreno,Ricardo D.
Mammalian germ cell apoptosis plays a key role in controlling the correct number of germ cells supported by Sertoli cells during the first wave of spermatogenesis in mammalian puberty. However, little is known about hormonal factors that could influence the rate of germ cell apoptosis during puberty or adulthood. In this work we evaluate germ cell apoptosis under hypothyroidism induced by goitrogen propylthiouracil (PTU) during the first wave of spermatogenesis. Neonatally administered PTU promoted a delay in the differentiation of Sertoli cells as evaluated by the expression of clusterin using immunohistochemistry and RT-PCR. Clusterin had different expression levels in control and PTU-treated animals, but under both conditions the highest levels were...
Tipo: Journal article
Palavras-chave: Testis; Spermatocyte; Sertoli cell; Caspase; Apoptosis; Thyroid.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200010

Quantitative analysis of nucleolar chromatin distribution in the complex convoluted nucleoli of Didinium nasutum (Ciliophora)
Provedor de dados: 48
Autores: Leonova,Olga G; Karajan,Bella P; Ivlev,Yuri F; Ivanova,Julia L; Skarlato,Sergei O; Popenko,Vladimir I.
We have earlier shown that the typical Didinium nasutum nucleolus is a complex convoluted branched domain, comprising a dense fibrillar component located at the periphery of the nucleolus and a granular component located in the central part. Here our main interest was to study quantitatively the spatial distribution of nucleolar chromatin structures in these convoluted nucleoli. There are no "classical" fibrillar centers in D.nasutum nucleoli. The spatial distribution of nucleolar chromatin bodies, which play the role of nucleolar organizers in the macronucleus of D.nasutum, was studied using 3D reconstructions based on serial ultrathin sections. The relative number of nucleolar chromatin bodies was determined in macronuclei of recently fed, starved...
Tipo: Journal article
Palavras-chave: 3D reconstruction; Ciliates; Electron microscopy; Macronucleus; Nucleoli.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100010

LUCO
Provedor de dados: 48
Autores: VERA,CRISTIAN.
Tipo: Journal article
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200004

Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
Provedor de dados: 48
Autores: Quiroz,Karla A.; Berrios,Miguel; Carrasco,Basilio; Retamales,Jorge B.; Caligari,Peter D. S.; García-Gonzáles,Rolando.
Abstract  Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis.  Results: In this context, we studied the cultivation of meristems and establishment of a...
Tipo: Journal article
Palavras-chave: Fragaria chiloensis; Meristem culture; Plant growth regulators; Virus elimination; Plant morphogenesis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100404

Anti-inflammatory effects of Salvia plebeia R. Br extract in vitro and in ovalbumin-induced mouse model
Provedor de dados: 48
Autores: Hwan-Hee,Jang; Su-Yeon,Cho; Mi-Ju,Kim; Jung-Bong,Kim; Sung-Hyen,Lee; Mee-Young,Lee; Young-Min,Lee.
BACKGROUND: Asthma is an increasing global health problem, and novel strategies to prevent or ameliorate the condition are needed. Here, the effects of 80 % ethanol extracts of Salvia plebeia R. Br. (SE) on an induced inflammatory response were investigated RESULTS: Salvia plebeia R. Br. inhibited production of pro-inflammatory cytokines, such as TNF-&amp;#945; and IL-6, as well as nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. NO and pro-inflammatory cytokine production was suppressed more effectively by SE of the aerial parts (SE-A) than of the roots (SE-R) of S. plebeia. In BEAS-2B cells, both SE-A and SE-R inhibited the increase in production of the inflammatory cytokines IL-6 and IL-8. We also investigated the antiasthmatic effects of SE in an...
Tipo: Journal article
Palavras-chave: Anti-inflammatory; Asthma; Salvia plebeia.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100041

Response of the G2-prophase checkpoint to genotoxic drugs in lymphocytes from healthy individuals
Provedor de dados: 48
Autores: Pincheira,Juana; de la Torre,Consuelo; Rodríguez,Natalie; Valenzuela,Carlos Y.
We analyzed the in vitro effects of the anti-tumoral drugs doxorubicin, cytosine arabinoside and hydroxyurea on the G2-prophase checkpoint in lymphocytes from healthy individuals. At biologically equivalent concentrations, the induced DNA damage activated the corresponding checkpoint. Thus: i) there was a concentration-dependent delay of G2 time and an increase of both the total DNA lesions produced and repaired before metaphase and; ii) G2-checkpoint adaptation took place as chromosome aberrations (CAs) started to appear in the metaphase, indicating the presence of unrepaired double-strand breaks (DSBs) in the previous G2. The checkpoint ATM/ATR kinases are involved in DSB repair, since the recorded frequency of CAs increased when both kinases were...
Tipo: Journal article
Palavras-chave: DNA damage and repair; G2-prophase checkpoint adaptation; Premitotic DSBs and chromosomal aberrations; Anti-tumoral genotoxic drugs.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200010

Methanol extract from Vietnamese Caesalpinia sappan induces apoptosis in HeLa cells
Provedor de dados: 48
Autores: Hung,Tran Manh; Dang,Nguyen Hai; Dat,Nguyen Tien.
BACKGROUND: This study evaluated the cytotoxic activity of extracts from Caesalpinia sappan heartwood against multiple cancer cell lines using an MTT cell viability assay. The cell death though induction of apoptosis was as indicated by DNA fragmentation and caspase-3 enzyme activation. RESULTS: A methanol extract from C. sappan (MECS) showed cytotoxic activity against several of the cancer cell lines. The most potent activity exhibited by the MECS was against HeLa cells with an IC50value of 26.5&amp;#8201;±&amp;#8201;3.2 µg/mL. Treatment of HeLa cells with various MECS concentrations resulted in growth inhibition and induction of apoptosis, as indicated by DNA fragmentation and caspase-3 enzyme activation. CONCLUSION: This study is the first report of the...
Tipo: Journal article
Palavras-chave: Caesalpinia sappan; Leguminosae; MTT assay; DNA fragmentation; Caspase-3.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100020

Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings
Provedor de dados: 48
Autores: Águila,Luis; Ruedlinger,Jenny; Mansilla,Karina; Ordenes,José; Salvatici,Raúl; Campos,Rui Ribeiro de; Romero,Fernando.
BACKGROUND: Ruta graveolens L. (R. graveolens) is a medicinal plant employed in non-traditional medicines that has various therapeutic properties, including anthelmintic, and vasodilatory actions, among others. We evaluated the trachea-relaxant effects of hydroalcoholic extract of R. graveolens against potassium chloride (KCl)- and carbachol-induced contraction of rat tracheal rings in an isolated organ bath. RESULTS: The results showed that the airway smooth muscle contraction induced by the depolarizing agent (KCl) and cholinergic agonist (carbachol) was markedly reduced by R. graveolens in a concentration-dependent manner, with maximum values of 109 ± 7.9 % and 118 ± 2.6 %, respectively (changes in tension expressed as positive percentages of change in...
Tipo: Journal article
Palavras-chave: Ruta graveolens; Trachea-relaxant; Plant extract.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100028

Reinstatement of short-latency responses after asymptotic Pavlovian conditioning training by the presentation of an extraneous stimulus
Provedor de dados: 48
Autores: Vogel,Edgar H.
The purpose of this study was to examine whether the progressive disappearance of short-latency conditioned responses, or inhibition of delay, observed in Pavlovian conditioning with long inter-stimulus intervals, could be reverted by the presentation of a novel stimulus. In one experiment, two groups of rabbits received extensive training with a short (250 ms) or a long (1500 ms) tone that overlapped and terminated with a periorbital shock unconditioned stimulus. After training, the presentation of an extraneous stimulus prior to tone onset produced a reinstatement of short latency CRs in the group trained with the long CS, but did not affect CR latency in the group trained with the short CS. This finding is consistent with Pavlov's (1927) view that...
Tipo: Journal article
Palavras-chave: Inhibition of delay; Conditioning; Temporal discrimination; Eyeblink conditioning; Timing.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100008

The Gating of Polycystin Signaling Complex
Provedor de dados: 48
Autores: DELMAS,PATRICK.
Mutations in either polycystin-2 (PC2) or polycystin-1 (PC1) proteins cause severe, potentially lethal, kidney disorders (autosomal dominant polycystic kidney disease, ADPKD) and multiple extrarenal disease phenotypes. PC2, a member of the transient receptor potential channel superfamily and PC1, an orphan membrane receptor of largely unknown function, are thought to be part of a common signalling pathway. Here, I show that co-assembly of full-length PC1 with PC2 forms an ion channel signalling complex in which PC1 regulates PC2 channel gating through a structural rearrangement of the polycystin complex (Delmas et al., 2004a). These polycystin complexes function either as a receptor-cation channel or as a G-protein-coupled receptor. Thus, PC1 acts as a...
Tipo: Journal article
Palavras-chave: Polycystin; TRP channel; Sensory transduction; Calcium signaling; Polycystic Kidney Disease.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400026

Positive effects of Green Tea on hepatic dysfunction, lipid peroxidation and antioxidant defence depletion induced by cadmium
Provedor de dados: 48
Autores: HAMDEN,KHALED; CARREA U,SERGE; AYADI MARKI,FATMA; MASMOUDI,HATEM; EL FEKI,ABDELFATTAH.
Cadmium (Cd) is a highly toxic environmental and industrial cumulative pollutant that affeets many organs, especially the liver. The present study was designed to evalúate the antioxidant effect of green tea on cadmium-induced hepatic dysfunction and oxidative stress in rats. Adult male Wistar rats were administered cadmium by injection of 20 emoles /kg bw/ every 3 days for six months. This study revealed significant (p &lt; 0.05) liver dysfunction, lipid peroxidation and a decline in antioxidant enzyme activities in the liver of cadmium-treated rats compared to control animáis. Compared to control rats, the activities of lactate dehydrogenase (LDH), gammaglutamyl transferase (GGT), acid phosphatase (PAC), phosphatase alkaline (PAL), as well as bilirubin...
Tipo: Journal article
Palavras-chave: Cadmium; Rat liver dysfunction; Green tea; Antioxidant enzymes; Lipid peroxidation.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300009

Upregulation of liver inducible nitric oxide synthase following thyroid hormone preconditioning: suppression by N-acetylcysteine
Provedor de dados: 48
Autores: FERNÁNDEZ,VIRGINIA; TAPIA,GLADYS; VARELA,PATRICIA; CORNEJO,PAMELA; VIDELA,LUIS A.
3,3-5-L-Triiodothyronine (T3) exerts significant protection against ischemia-reperfusion (IR) liver injury in rats. Considering that the underlying mechanisms are unknown, the aim of this study was to assess the involvement of inducible nitric oxide synthase (iNOS) expression and oxidative stress in T3 preconditioning (PC). Male Sprague-Dawley rats given a single dose of 0.1 mg of T3/kg were subjected to 1-hour ischemia followed by 20 hours reperfusion, in groups of animals pretreated with 0.5 g of N-acetylcysteine (NAC)/kg 0.5-hour prior to T3 or with the respective control vehicles. At the end of the reperfusion period, liver samples were taken for analysis of iNOS mRNA levels (RT-PCR), liver NOS activity, and hepatic histology. T3 protected against...
Tipo: Journal article
Palavras-chave: Thyroid hormone; Oxidative stress; Inducible nitric oxide synthase; Liver preconditioning; Ischemia-reperfusion injury; N-acetylcysteine.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400010

The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
Provedor de dados: 48
Autores: Liu,Yu; Wang,Lu; Liu,Heng; Zhao,Rongrong; Liu,Bin; Fu,Quanjuan; Zhang,Yuanhu.
BACKGROUND: &amp;#945;-Farnesene is a volatile sesquiterpene synthesized by the plant mevalonate (MVA) pathway through the action of &amp;#945;-farnesene synthase. The &amp;#945;-farnesene synthase 1 (MdAFS1) gene was isolated from apple peel (var. white winterpearmain), and transformed into tobacco (Nicotiana tabacum NC89). The transgenic plants had faster stem elongation during vegetative growth and earlier flowering than wild type (WT). Our studies focused on the transgenic tobacco phenotype. RESULTS: The levels of chlorophyll and soluble protein decreased and a lower seed biomass and reduced net photosynthetic rate (Pn) in transgenic plants. Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide radicals (O2._) had higher levels...
Tipo: Journal article
Palavras-chave: Tobacco; Senescence; Transcriptome; Reactive oxygen species (ROS).
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100030

Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk
Provedor de dados: 48
Autores: SOUZA VIANA,ELISETH; MARTINO CAMPOS,MARIA EMILENE; REIS PONCE,ADRIANA; CUQUETTO MANTOVANI,HILÁRIO; DANTAS VANETTI,MARIA CRISTINA.
The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well diffusion techniques, thin layer chromatography (TLC) and detection directly in biofilm with biomonitors. The extracts obtained from planktonic and sessile cell of H. alvei induced at least two of three monitor strains evaluated. The presence of AHLs with up to six carbon atoms was confirmed by TLC. Biofilm formation by H. alvei was inhibited by furanone, as demonstrated by 96-well assay of crystal violet in microtitre plates and by scanning electron microscopy....
Tipo: Journal article
Palavras-chave: Acyl homoserines lactones; Biofilm; Furanones; Quorum sensing.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400004

Immune enhancement activities of silk lutein extract from Bombyx mori cocoons
Provedor de dados: 48
Autores: Promphet,Porkaew; Bunarsa,Sirirat; Sutheerawattananonda,Manote; Kunthalert,Duangkamol.
BACKGROUND: Declining immune function poses an important clinical challenge worldwide and supplementation with natural products that possessing immune enhancing properties is a promising approach for preventing or delaying immune function decline. Cocoons from yellow silkworms are a significant source of lutein, and this unexplored silk extract could be a viable alternative source for dietary lutein. This study assessed immunomodulatory activities of the silk lutein extract. Female BALB/c mice orally received lutein, either as silk or marigold extracts (10 or 20 mg/kg daily), or vehicle only (1% tween 80 in PBS pH 7.4) for 4 weeks. Natural killer (NK) cell activity, specific antibody production, lymphocyte subpopulations, mitogen-induced lymphocyte...
Tipo: Journal article
Palavras-chave: Antibody production; BALB/c mice; Immune function; Interleukin-2; Natural killer cells; Silk lutein extract.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100015

Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
Provedor de dados: 48
Autores: BOVERIS,ALEJANDRO D; GALATRO,ANDREA; PUNTARULO,SUSANA.
The antioxidant ability of nitric oxide (NO) generated by a chemical donor and of commercially available antioxidant preparations was assayed. SNAP (S-Nitroso-N-acetylpenicilamine) was used as the NO donor, and Ginkgo biloba, wheat and alfalfa preparations were tested. Lipid peroxidation was assayed by EPR employing a reaction system consisting of rat liver microsomes, ADP, FeCl3, NADPH and POBN in phosphate buffer, pH=7.4. In vitro NO exposure decreased microsomal lipid peroxidation in a dose-dependent manner. The dose responsible for inhibiting the microsomal content of lipid radical adducts by 50% (LD50) for SNAP was 550 mu M (NO generation rate 0.1 mu M/min). The addition of 50 mM hemoglobin to the incubation media prevented NO effect on lipid...
Tipo: Journal article
Palavras-chave: Antioxidants; Nitric oxide; Plants extracts; Lipid radicals.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200016

Sugars, organic acids, and phenolic compounds of ancient grape cultivars (Vitis vinifera L.) from Igdir province of Eastern Turkey
Provedor de dados: 48
Autores: Eyduran,Sadiye Peral; Akin,Meleksen; Ercisli,Sezai; Eyduran,Ecevit; Maghradze,David.
BACKGROUND: The Eurasian grapevine (Vitis vinifera L.) is the most widely cultivated and economically important horticultural crop in the world. As a one of the origin area, Anatolia played an important role in the diversification and spread of the cultivated form V. vinifera ssp. vinifera cultivars and also the wild form V. vinifera ssp. sylvestris ecotypes. Although several biodiversity studies have been conducted with local cultivars in different regions of Anatolia, no information has been reported so far on the biochemical (organic acids, sugars, phenolic acids, vitamin C) and antioxidant diversity of local historical table V. vinifera cultivars grown in Igdir province. In this work, we studied these traits in nine local table grape cultivars viz....
Tipo: Journal article
Palavras-chave: Table grape; Biochemical composition; HPLC; Spectrophotometer; Germplasm characterization.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100002

Sleep in brain development
Provedor de dados: 48
Autores: PEIRANO,PATRICIO D; ALGARÍN,CECILIA R.
With the discovery of rapid eye movement (REM) sleep, sleep was no longer considered a homogeneous state of passive rest for the brain. On the contrary, sleep, and especially REM sleep, appeared as an active condition of intense cerebral activity. The fact that we get large amounts of sleep in early life suggested that sleep may play a role in brain maturation. This idea has been investigated for many years through a large number of animal and human studies, but evidence remains fragmented. The hypothesis proposed was that REM sleep would provide an endogenous source of activation, possibly critical for structural maturation of the central nervous system. This proposal led to a series of experiments looking at the role of REM sleep in brain development. In...
Tipo: Journal article
Palavras-chave: Sleep development; Brain development; Brain plasticity; NREM sleep; REM sleep; Introducing sleep.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000500008

Cytotoxicity, nitric oxide and acetylcholinesterase inhibitory activity of three limonoids isolated from Trichilia welwitschii (Meliaceae)
Provedor de dados: 48
Autores: Dzoyem,Jean P.; Tsamo,Armelle T.; Melong,Raduis; Mkounga,Pierre; Nkengfack,Augustin E.; McGaw,Lyndy J.; Eloff,Jacobus N.
BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 (&amp;#956;g/L. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin...
Tipo: Journal article
Palavras-chave: Trichilia welwitschii; Cytotoxicity; Acetylcholinesterase; Nitric oxide; Limonoids.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100057

Proteomic Investigation of Changes in Rat Skeletal Muscle after Exercise-Induced Fatigue
Provedor de dados: 48
Autores: Zhao,Liping; Yan,Wenhui; Xiang,Heng; Wang,Xiaoyang; Qiao,Haixuan.
The mechanisms of exercise-induced fatigue have not been investigated using proteomic techniques, an approach that could improve our understanding and generate novel information regarding the effects of exercise. In this study, the proteom alterations of rat skeletal muscle were investigated during exercise-induced fatigue. The proteins were extracted from the skeletal muscle of SD rat thigh, and then analyzed by two-dimensional electrophoresis and PDQuest software. Compared to control samples, 10 significantly altered proteins were found in exercise samples, two of them were upregulated and eight of them were downregulated. These proteins were identified by MALDI TOF-MS. The two upregulated proteins were identified as MLC1 and myosin L2 (DTNB) regulatory...
Tipo: Journal article
Palavras-chave: Proteomics; Exercise-induced fatigue; Skeletal muscle; Rat.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100010

MI HOMENAJE GUAYO ROJAS, COMPAÑERO DE RUTA
Provedor de dados: 48
Autores: VERDUGO,PEDRO.
Tipo: Journal article
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300002

Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
Provedor de dados: 48
Autores: Cohen,Débora J; Maldera,Julieta A; Weigel Muñoz,Mariana; Ernesto,Juan I; Vasen,Gustavo; Cuasnicu,Patricia S.
Epididymal protein CRISPI is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Results from our laboratory have shown that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, our results revealed that CRISP1 could also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mouse line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice...
Tipo: Journal article
Palavras-chave: Sperm; Gamete fusion; CRISP; Egg; Fertilization.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200004