Consulta: subjectFacets:"Characterization"
Registros recuperados: 101
Data/hora: 07/06/2024 02:37:33

Caracterización de la cadena de suministros de la miel en el estado de Tabasco.
Provedor de dados: 32
Autores: Pascual González, Josué.
El presente trabajo presenta el resultado de  la Caracterización de la Cadena de Suministros de la Miel (CSM) en el Estado de Tabasco y la Evaluación de Sistemas de Trazabilidad para la Miel.  La CSM está integrada por una serie relaciones ascendentes y descendentes entre unidades de producción, para una fácil compresión en este trabajo, a las unidades de producción se les ha denominado actores de CSM. La caracterización  de la CSM en el estado de Tabasco se realizó entre proveedores de materias primas e insumos, apicultores, intermediarios, industrializadores y consumidores; posteriormente se les clasificó de acuerdo al Sistema de Clasificación para América del Norte. Se utilizó el marco metodológico exploratorio, propuesto por Hernández (1998) como...
Palavras-chave: Caracterización; Cadena de suministro de la miel; Actores de cadena de suministro; Characterization; Supply chain honey supply; Producción Agroalimentaria en el Trópico; Maestría.
Ano: 2012
URL: http://hdl.handle.net/10521/1697

Caracterización del sistema de producción de mamey (Pouteria sapota Jacq.) del Municipio de Huamuxtitlán, Guerrero.
Provedor de dados: 32
Autores: Arcos López, Enrique.
El presente estudio se realizó en el municipio de Huamuxtitlán, Guerrero, México. El objetivo del estudio fue analizar y caracterizar los sistemas de producción del cultivo de mamey. Se aplicó un cuestionario a 25 productores de mamey en las comunidades del municipio. Se encontró que los productores de mamey tienen una edad promedio de 53.7 años, su escolaridad promedio es de 5.76 años y tienen una experiencia promedio de 18.76 años. Los principales problemas que presenta el cultivo de mamey son la falta de organización de los productores y la incidencia de la mosca de la fruta. La tecnología utilizada en el cultivo de mamey está  por debajo de la recomendada. El análisis de componentes principales indicó que las principales variables que caracterizan al...
Palavras-chave: Mamey; Sistema de Producción; Caracterización; Mamey sapote; Production system; Characterization; Desarrollo Sostenible de Zonas Indígenas; EDAR; Maestría Tecnológica.
Ano: 2011
URL: http://hdl.handle.net/10521/443

Infestación por garrapatas en el ganado criollo lechero tropical en Veracruz, México
Provedor de dados: 32
Autores: González Cerón, Fernando.
El presente estudio tuvo como objetivo caracterizar la infestación por garrapatas en un hato experimental de ganado Criollo Lechero Tropical (CLT) ubicado en la región central del estado de Veracruz, México; la información generada permitiría iniciar el registro sistemático de la infestación causada por éstos ectoparásitos para considerarla como una posible característica a ser incluida en un programa de mejora genética a mediano plazo. A 28 vaquillas (655±35 días de edad) y 16 toretes (741±89 días de edad) CLT se les retiraron del cuerpo todas las garrapatas saturadas (≥4.5 mm) considerando 18 regiones corporales para identificar la especie a la que pertenecían, ubicar las regiones corporales donde se localizaban, determinar la carga parasitaria y estimar...
Tipo: Tesis
Palavras-chave: Criollo lechero tropical; Garrapata; Caracterización; Maestría; Ganadería; Tropical milking criollo; Tick; Characterization.
Ano: 2007
URL: http://hdl.handle.net/10521/1623

Caracterización fitosanitaria de las plantaciones del programa de incentivos forestales (PINFOR) en los departamentos de alta Verapaz y el Petén, Guatemala
Provedor de dados: 32
Autores: Cordón Cabrera, Pablo Raúl.
Este trabajo presenta la primera exploración de plagas forestales, realizada  durante el período Septiembre 2006 a Marzo 2007  en plantaciones que han sido  beneficiadas por el programa de incentivos forestales PINFOR. Se hicieron  recorridos terrestres a las plantaciones seleccionadas, realizando muestreos con  intervalos de 15 días, en los que se identificaron árboles con síntomas anormales.  y se verificó la presencia de insectos y/o árboles con enfermedades. Los insectos  fueron recolectados en frascos con alcohol al 70% y en el caso de las  enfermedades se confinaron partes vegetales en  bolsas de plástico con  condiciones de humedad. Para la identificación de los organismos se utilizaron  claves  taxonómicas entomológicas y fitopatológicas del...
Tipo: Tesis
Palavras-chave: Plagas y enfermedades forestales; PINFOR; Identificación; Maestría; Entomología y Acarología; Forest pest and disease; Characterization; Identification.
Ano: 2009
URL: http://hdl.handle.net/10521/1339

Caracterización agronómica, bromatológica, isoenzimática y radiosensibilidad de poblaciones de amaranto (Amaranthus spp.) colectadas en las principales área de producción de México.
Provedor de dados: 32
Autores: Subía García, Cristian Roberto.
El amaranto (Amaranthus spp.) es una especie originaria de América con gran valor agronómico, nutricional, de mucho potencial económico y ampliamente dispersada. El presente estudio se realizó con el objetivo de caracterizar poblaciones nativas procedentes de los estados centrales de México y dos especies sudamericanas. La investigación se realizó en cuatro fases: agronómica, bromatológica, genética y se evaluó la tolerancia a la radiación. Se observó amplia variación entre poblaciones y grupos de origen con base en los métodos de análisis empleado y los métodos de agrupamiento separaron las especies de acuerdo a su origen. Las características agronómicas de mayor importancia para diferenciar a las poblaciones fueron: días a la floración y a la madurez,...
Palavras-chave: Amaranthus; Caracterización; Agronómica; Bromatología; Isoenzimas; Radiosenbilidad; Genética; Maestría; Characterization; Agronomical; Bromatological; Isoenzimes; Radiation sensitibility.
Ano: 2012
URL: http://hdl.handle.net/10521/1785

Diversidad y caracterización de poblaciones bacterianas asociadas a esclerocios de Claviceps gigantea Fuentes et al. en maíz (Zea mays L.) con potencial antagonista, en el Estado de México.
Provedor de dados: 32
Autores: Ayala Torres, Ana María.
Un total de 129 aislamientos bacterianos obtenidos de la micósfera de esclerocios de Claviceps gigantea se evaluaron in vitro para determinar su capacidad de inhibir el crecimiento de Fusarium graminearum, F. subglutinans y F. verticillioides patógenos a maíz. Ensayos cualitativos en confrontación dual, resultaron en la selección de 22 aislamientos bacterianos con el mayor potencial antagónico hacia una, dos o las tres especies de Fusarium. Los aislamientos se identificaron molecularmente mediante secuenciación parcial del gen 16s ADNr. Las cepas con mayor grado de antagonismo pertenecen a los géneros Bacillus y Pseudomonas. La caracterización en la producción de metabolitos potencialmente involucrados en la actividad antagónica, mostró que el 100% de las...
Palavras-chave: Bacillus; Pseudomonas; Biocontrol; Metabolitos secundarios; Caracterización; Secondary metabolites; Characterization; Fitopatología; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2299

Caracterización fitosanitaria de las plantaciones del programa de incentivos forestales (PINFOR) en los departamentos de alta verapaz y el petén, Guatemala
Provedor de dados: 32
Autores: Córdova Cabrera, Pablo Raúl.
Este trabajo presenta la primera exploración de plagas forestales, realizada  durante el período Septiembre 2006 a Marzo 2007  en plantaciones que han sido  beneficiadas por el programa de incentivos forestales PINFOR. Se hicieron  recorridos terrestres a las plantaciones seleccionadas, realizando muestreos con  intervalos de 15 días, en los que se identificaron árboles con síntomas anormales.  y se verificó la presencia de insectos y/o árboles con enfermedades. Los insectos  fueron recolectados en frascos con alcohol al 70% y en el caso de las  enfermedades se confinaron partes vegetales en  bolsas de plástico con  condiciones de humedad. Para la identificación de los organismos se utilizaron  claves  taxonómicas entomológicas y fitopatológicas del...
Tipo: Tesis
Palavras-chave: Plagas y enfermedades forestales; PINFOR; Identificación Forest pest and disease; PINFOR; Characterization; Identification.
Ano: 2012
URL: http://hdl.handle.net/10521/1040

Morfología de frutos, perfil de ácidos grasos en semilla y diversidad genética molecular en selecciones de zapote mamey (Pouteria sapota (Jacq.) H. E. Moore &amp; Stearn).
Provedor de dados: 32
Autores: Ibarra Estrada, Martha Elva.
A México se le considera   centro de origen del zapote mamey ya que cuenta con una gran diversidad. Sin embargo, son pocos   los trabajos enfocados en estudiar tal diversidad tanto en la parte morfológica, contenido de ácidos graso o aspectos moleculares. Por lo anterior, el objetivo   del presente trabajo fue: caracterizar morfológica, bioquímica y molecularmente selecciones sobresalientes de zapote mamey. En base a las variables de fruto y el perfil de ácidos grasos en semilla, el material vegetal estudiado mostró amplia variación entre las accesiones. El peso y longitud de fruto, longitud de hilio y de semilla, número de fibras, grosor de epicarpio y ancho de semilla fueron las variables determinantes para la agrupación de las selecciones. El perfil de...
Palavras-chave: Caracterización; Frutos; Variabilidad; RAPD; RAMP; Characterization; Fruits; Lipids; Seeds; Variability; Lípidos; Semillas; Doctorado; Fruticultura.
Ano: 2012
URL: http://hdl.handle.net/10521/740

Caracterización agronómica, nutrimental y molecular de Vicia faba L.
Provedor de dados: 32
Autores: List Montesinos, Hildegard Berenice.
El haba es un cultivo de alto valor nutrimental, es la tercer leguminosa consumida a nivel nacional, después del frijol y la lenteja, cultivándose principalmente en los estados de Puebla, Tlaxcala, Veracruz, Estado de México y Michoacán. Sin embargo, el empleo de variedades criollas con baja producción y susceptibles a enfermedades ha propiciado la disminución de la superficie destinada a su cultivo. El objetivo de la investigación fue la caracterización agronómica, calidad culinaria, nutrimental y molecular de tres colectas  de V. faba L. (tipo minor  y tipo equina) provenientes de localidades con diferente altitud ubicadas en Puebla y Oaxaca, con el fin de identificar líneas sobresalientes para iniciar un programa de mejoramiento genético. En la parte...
Palavras-chave: Vicia faba tipo minor; Equina; Caracterización; Formación de líneas; Vicia faba minor; Characterization; Selection lines; Genética; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2330

Caracterización morfológica de genotipos de pitahaya (Hylocereus spp.).
Provedor de dados: 32
Autores: Martínez Hernández, Gregorio.
Las pitahayas son utilizadas desde tiempos prehispánicos; sin embargo, solo desde hace poco más de dos décadas se han establecido como cultivo, con gran potencial para coadyuvar al desarrollo agrícola y económico de México y otros países. Conocer la diversidad de un recurso vegetal permite elegir estrategias para su mejor aprovechamiento. Por tal motivo, el propósito de este trabajo fue la caracterización morfológica de 21 genotipos de pitahaya (Hylocereus spp.) cultivados en Tepoztlán Morelos, México. La caracterización morfológica se realizó con 28 caracteres cuantitativos y 22 cualitativos de yemas florales, flores, filocladodios y frutos. Con base en análisis de componentes principales los genotipos se diferenciaron principalmente por caracteres de...
Palavras-chave: Hylocereus spp.; Caracterización; Genotipos; Pitahaya; Characterization; Genotypes; Genética; Maestría.
Ano: 2013
URL: http://hdl.handle.net/10521/2131

Hábitat y distribución potencial del tecolote moteado mexicano (Strix occidentalis lucida) en Tlachichila, Zacatecas, y áreas adyacentes.
Provedor de dados: 32
Autores: Palma Cancino, Davira Yolanda.
El tecolote moteado mexicano es una especie amenazada, en Estados Unidos y México. En México esta especie ha sido poco estudiada. Para localizar, registrar y caracterizar el hábitat de los sitios de descanso del tecolote moteado mexicano, así como para determinar su distribución potencial en áreas adyacentes, se condujeron muestreos en Tlachichila, Zacatecas. Para ello, se establecieron estaciones de llamado nocturnos en la que se emplearon imitaciones de sus vocalizaciones y búsquedas durante el día. Se muestrearon 7, 639 ha, y se localizaron cinco individuos subadultos en tres sitios de descanso (dos parejas y un individuo solitario). La caracterización del hábitat se realizó en sitios de uso y sitios aleatorios, en parcelas circulares de 20 m de...
Palavras-chave: Strix occidentales lucida; Caracterización; Hábitat potencial; Maxent; Characterization; Potential habitat; Ganadería; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2369

Caracterización de tipos de maíz criollo y sistemas de cultivo : estudio de caso en tres comunidades de la Costa Chica de Guerrero
Provedor de dados: 32
Autores: Hernández Flores, Martín.
La investigación ha privilegiado la identificación y caracterización de un bien histórico de gran importancia patrimonial y valor presente, que se manifiesta como factor fundamental a través de un conjunto de poblaciones de maíces nativos -conocidos localmente como criollos-, al interior de una amplia diversidad y multifuncionalidad de sistemas productivos, en la región de la Costa Chica de Guerrero. La tendencia de la modernización de la agricultura durante los últimos años, demuestra que en la región se ha excluido sistemáticamente el valor presente de los recursos locales, los cuales indudablemente forman parte de las lógicas de funcionamiento de los sistemas de producción campesinos e indígenas. En particular, ha sido y es notorio el desinterés...
Tipo: Tesis
Palavras-chave: Tipos de maíz criollo; Sistemas de cultivo; Caracterización; Maestría; Desarrollo Rural; Types of creole maize; Systems of culture; Characterization.
Ano: 2007
URL: http://hdl.handle.net/10521/1557

Caracterización socioeconómica, morfológica y bioquímica de una muestra etnográfica de maíz (Zea mays L.) raza bolita.
Provedor de dados: 32
Autores: Ramírez Jaspeado, Araceli.
El maíz es el cultivo más importante de México cuya superficie de siembra es de 8 millones de hectáreas con un rendimiento promedio de 3 ton/ha. Su aprovechamiento involucra los granos, tallos, espigas, raíces, olote y brácteas (“hoja para tamal”) utilizados para diversos propósitos en la alimentación humana, animal, medicinal e industrial. Se ha considerado que la principal forma de consumo del maíz es a través de la tortilla, donde, alrededor de 12.3 millones de toneladas de maíz se consumen en esta forma. En este sentido, los maíces mejorados han sido esenciales para dicho propósito, sin embargo, el uso de los maíces nativos o criollos, también ha sido de gran importancia ya que son un reservorio de la variabilidad genética y han sido la base de la...
Palavras-chave: Caracterización; Raza Bolita; Tlayuda; Cadena de valor; Calidad nutricional; Proteínas transgénicas; Conservación; Producción de semillas; Doctorado; Characterization; Bolita race; Value chain; Nutritional quality; Transgenic proteins; Conservation.
Ano: 2012
URL: http://hdl.handle.net/10521/1822

Diversidad de los recursos genéticos mexicanos del hongo funcional ganoderma (Fungi, Ganodermataceae), conocido como reishi en los mercados internacionales, y su relevancia para el desarrollo regional.
Provedor de dados: 32
Autores: Tello Salgado, Isaac.
Actualmente se estudia la capacidad funcional a nivel molecular de los hongos basidiomicetos como modificadores de la respuesta biológica, dada la efectividad de los productos examinados de activar al sistema inmunológico, sin embargo, el origen geográfico de estos recursos, principalmente son asiáticos y europeos. Ganoderma es reconocido por su eficiente actividad en el tratamiento de varias enfermedades, principalmente las anticancerígenas y antitumorales, crece de manera silvestre en el área central de México, una de las más importantes, debido a la convergencia de las regiones Neártica y Neotropical, lo cual da como resultado un amplia diversidad de recursos genéticos. En la presente investigación se aislaron 66 cepas del género Ganoderma producto de...
Palavras-chave: Caracterización; Cultivo; Filogenia; Ganoderma; Recurso Genético; Characterization; Cultivation; Phylogeny; Genetic resource; Doctorado; EDAR; Estrategias para el Desarrollo Agrícola Regional.
Ano: 2010
URL: http://hdl.handle.net/10521/372

Caracterización del sistema de producción de mamey (Pouteria sapota Jacq.) del Municipio de Huamuxtitlán, Guerrero.
Provedor de dados: 32
Autores: Arcos López, Enrique.
El presente estudio se realizó en el municipio de Huamuxtitlán, Guerrero, México. El objetivo del estudio fue analizar y caracterizar los sistemas de producción del cultivo de mamey. Se aplicó un cuestionario a 25 productores de mamey en las comunidades del municipio. Se encontró que los productores de mamey tienen una edad promedio de 53.7 años, su escolaridad promedio es de 5.76 años y tienen una experiencia promedio de 18.76 años. Los principales problemas que presenta el cultivo de mamey son la falta de organización de los productores y la incidencia de la mosca de la fruta. La tecnología utilizada en el cultivo de mamey está  por debajo de la recomendada. El análisis de componentes principales indicó que las principales variables que caracterizan al...
Palavras-chave: Mamey; Sistema de Producción; Caracterización; Mamey sapote; Production system; Characterization; Desarrollo Sostenible de Zonas Indígenas; EDAR; Maestría Tecnológica.
Ano: 2011
URL: http://hdl.handle.net/10521/443

Distinción, homogeneidad y estabilidad (DHE) mediante caracterización morfológica y molecular en variedades de amaranto.
Provedor de dados: 32
Autores: Ramírez, Ma. Elena.
Con el objetivo de valorar procedimientos para la evaluación de distinción, homogeneidad y estabilidad en variedades vegetales con fines de protección a los derechos del obtentor, se utilizaron 10 variedades de amaranto. Para el examen de distinción, se aplicó el sistema de agrupamiento del Servicio Nacional de Inspección y Certificación de Semillas, además del Sistema Interanual de Distinción para seis caracteres cuantitativos, destacando que la calificación que se otorga a estos caracteres, no corresponde con los resultados obtenidos aplicando la diferencia mínima significativa. En cuanto a la homogeneidad, todas las plantas de una variedad resultaron muy parecidas entre sí; por otra parte, la estabilidad se evaluó mediante la homogeneidad de los...
Palavras-chave: Amaranto; Caracterización; Protección de variedades; DHE; Intermicrosatélites; Amaranth; Characterization; Variety protection; DUS; Inter-microsatellite; Doctorado; Producción de Semillas.
Ano: 2011
URL: http://hdl.handle.net/10521/473

Distinción, homogeneidad y estabilidad (DHE) mediante caracterización morfológica y molecular en variedades de amaranto.
Provedor de dados: 32
Autores: Ramírez, Ma. Elena.
Con el objetivo de valorar procedimientos para la evaluación de distinción, homogeneidad y estabilidad en variedades vegetales con fines de protección a los derechos del obtentor, se utilizaron 10 variedades de amaranto. Para el examen de distinción, se aplicó el sistema de agrupamiento del Servicio Nacional de Inspección y Certificación de Semillas, además del Sistema Interanual de Distinción para seis caracteres cuantitativos, destacando que la calificación que se otorga a estos caracteres, no corresponde con los resultados obtenidos aplicando la diferencia mínima significativa. En cuanto a la homogeneidad, todas las plantas de una variedad resultaron muy parecidas entre sí; por otra parte, la estabilidad se evaluó mediante la homogeneidad de los...
Palavras-chave: Amaranto; Caracterización; Protección de variedades; DHE; Intermicrosatélites; Amaranth; Characterization; Variety protection; DUS; Inter-microsatellite; Doctorado; Producción de Semillas.
Ano: 2011
URL: http://hdl.handle.net/10521/473

Variación agromorfológica e identidad taxonómica del romerito (Suaeda spp.).
Provedor de dados: 32
Autores: Noguez Hernández, Roberto.
En la presente investigación, se realizó una caracterización agromorfológica y se identificó taxonómicamente una nueva especie de romerito. Para el cumplimiento de los objetivos, este trabajo se dividió en dos capítulos. En el primero, a partir de caracteres cualitativos y cuantitativos, se caracterizaron y agruparon 70 muestras poblacionales procedentes de plantas cultivadas y silvestres.  Los resultados de la investigación a partir de los análisis de correspondencias y discriminante permitieron agrupar la variación del romerito de acuerdo a la zona de colecta en tres grupos. (Centro, Occidente y Norte). Los caracteres ciclo de vida, número de estambres, tipo de crecimiento en etapa vegetativa, días a floración, fructificación y madurez de semilla fueron...
Palavras-chave: Romerito; Caracterización; Sueada edulis; Suaeda mexicana; Comestible; Characterization; Edible; Producción de Semillas; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2107

Preparation and characterization of &amp;#954;-carrageenase immobilized onto magnetic iron oxide nanoparticles
Provedor de dados: 69
Autores: Xiao,Anfeng; Xu,Caiyun; Lin,Yan; Ni,Hui; Zhu,Yanbing; Cai,Huinong.
Background Carboxyl-functionalized magnetic nanoparticles were synthesized via chemical co-precipitation method and modified with oleic acid which was oxidized by potassium permanganate, and &amp;#954;-carrageenase from Pseudoalteromonas sp. ASY5 was subsequently immobilized onto them. The immobilization conditions were further optimized, and the characterizations of the immobilized &amp;#954;-carrageenase were investigated. Results The &amp;#954;-carrageenase was immobilized onto magnetic iron oxide nanoparticles, and the bonding was verified by Fourier transform infrared spectroscopy. The optimal conditions for &amp;#954;-carrageenase immobilization were 2.5% (w/v) glutaraldehyde, 13.9 U &amp;#954;-carrageenase for 20 mg of magnetic nanoparticles, a 2-h...
Tipo: Journal article
Palavras-chave: Carrageenase; Characterization; Immobilization; Nanoparticles.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100001

Genetic variability for waxy genes in Argentinean bread wheat germplasm
Provedor de dados: 69
Autores: Vanzetti,Leonardo Sebastián; Pflüger,Laura Alicia; Rodríguez-Quijano,Marta; Carrillo,José Maria; Helguera,Marcelo.
Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with the lack of waxy protein(s). In this work, different sets of PCR markers were used to characterize the genetic variability of waxy loci from 103 Argentinean bread wheat cultivars. For the Wx-A1 locus, Wx-A1a and a novel molecular allele designed Wx-A1g were detected. Wx-B1 locus showed three alleles (Wx-B1a, Wx-B1b, Wx-B1e), and Wx-D1 locus showed only the Wx-D1a allele. Novel single-locus allele specific markers for Wx-A1b, Wx-B1b and Wx-D1b null alleles were...
Tipo: Journal article
Palavras-chave: Characterization; Molecular markers; Triticum aestivum L.; Starch; Waxy genes; Wheat.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100004

Thermostable lipase from a newly isolated Staphylococcus xylosus strain; process optimization and characterization using RSM and ANN
Provedor de dados: 69
Autores: Khoramnia,Anahita; Lai,Oi Ming; Ebrahimpour,Afshin; Tanduba,Carynn Josue; Voon,Tan Siow; Mukhlis,Suriati.
Normal feed forward back-propagation artificial neural network (ANN) and cubic backward elimination response surface methodology (RSM) were used to build a predictive model of the combined effects and optimization of culture parameters for the lipase production of a newly isolated Staphylococcus xylosus. The results demonstrated a high predictive accuracy of artificial neural network compared to response surface methodology. The optimum operating condition obtained from the ANN model was found to be at 30ºC incubation temperature, pH 7.5, 60 hrs incubation period, 1.8% inoculum size and 60 rpm agitation. The lipase production increased 3.5 fold for optimal medium. The produced enzyme was characterized biochemically and this is the first report about a...
Tipo: Journal article
Palavras-chave: Artificial neural network; Characterization; Lipase; Optimization; Response surface methodology; Staphylococcus xylosus.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500015

Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains
Provedor de dados: 69
Autores: Lin,Ling; Kan,Xianzhao; Yan,Hao; Wang,Danni.
The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from...
Tipo: Journal article
Palavras-chave: Bacillus thuringiensis; Cellulase; Cellulose-degrading; Characterization.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002

EVALUATION OF THE Peltophorum vogelianum Benth. WOOD SPECIES FOR STRUCTURAL USE
Provedor de dados: 70
Autores: Christoforo,André L.; Aquino,Vinicius B. de M.; Wolenski,Anderson R. V.; Araujo,Victor A. De; Lahr,Francisco A. R.
ABSTRACT Wood is a material used for various purposes since ancient times and is mainly used in civil and rural construction and industry. Due to the predatory exploitation of known trees, it is necessary to characterize new species for use in urban and rural structures as alternatives of species commonly used. This study aimed to determine the physical and mechanical properties of Guarucaia wood (Peltophorum vogelianum Benth.) based on the Brazilian standard ABNT NBR 7190 (1997) and estimate the strength and stiffness as a function of apparent density using the analysis of variance and also estimate stiffness as a function of its strength. The mechanical properties of P. vogelianum were considered compatible for structural use when compared with others...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peltophorum vogelianum; Characterization; Apparent density; Regression models; Analysis of variance (ANOVA).
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162019000600763

ANALYSIS OF ELASTICITY IN WOODS SUBMITTED TO THE STATIC BENDING TEST USING THE PARTICLE IMAGE VELOCIMETRY (PIV) TECHNIQUE
Provedor de dados: 70
Autores: Pereira,Rodrigo A.; Gomes,Francisco C.; Braga Júnior,Roberto A.; Rivera,Fernando P.
ABSTRACT The most important parameter in terms of material mechanical behavior knowledge is the modulus of elasticity, being traditionally obtained through destructive tests. The objective of this study is the verification of the potential use of the particle image velocimetry technique (PIV) as a tool to obtain the modulus of elasticity in sawed wood samples (Pinus oocarpa and Eucaliptus grandis) and wood panels (Plywood, LVL and OSB). The PIV technique has as characteristics the low cost of equipment, fast results, no need for contact with the object tested, accuracy and possibility of application in the field. The application of the PIV technique occurred during the static bending tests where the deformations were also measured with a dial indicator...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Nondestructive testing; Modulus of elasticity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162018000200159

Genetic characterization of Aberdeen Angus cattle using molecular markers
Provedor de dados: 74
Autores: Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida.
Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005

Characterization of different strains of common carp (Cyprinus carpio L.) (Cyprinidae, Cypriniformes) in Bangladesh using microsatellite DNA markers
Provedor de dados: 74
Autores: Mondol,Rashedul Kabir; Islam,Shahidul; Alam,Samsul.
Characterization of different strains of common carp (Cyprinus carpio L.) using molecular markers is essential for the management of this fish in respect to the evaluation of the potential genetic effects induced by hatchery operations and the genetic improvement of carp varieties. Five microsatellite loci (MFW1, MFW2, MFW11, MFW15 and MFW20) were analyzed for the molecular characterization of four common carp strains, i.e. scaled carp, mirror carp, red carp and koi carp. We observed differences in heterozygosities and the average numbers of alleles but not in polymorphic loci (P95) among the strains. Koi carp displayed the highest level of variability in terms of heterozygosity. The Nm values and the F ST values indicated a low level of gene flow and high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Cyprinus carpio; Microsatellite marker.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400009

Germination and hardseedness of seeds in okra elite lines
Provedor de dados: 75
Autores: Purquerio,Luis Felipe V; Lago,Antonio A do; Passos,Francisco Antonio.
The occurrence of hardseedness and the low percentage of seed germination are major challenges when growing okra. For this reason, five elite lines of okra developed at the Campinas Agronomic Institute (IAC), three with angular pods (lines 8.1, 20.1.2 and 47.1) and two with cylindrical pods (lines 13.1.2 and 20.1.R), were evaluated for seed quality and compared to two commercial seed samples (Horticeres Sementes and Sakata Seed Sudamerica) of cultivar Santa Cruz 47. Seeds were field produced in an experiment set in the Technological Park of the Horticulture Center of IAC, in Campinas, São Paulo State, Brazil, in a randomized block design, with three replications and 20-plant plots. Fruits were harvested 25, 35, 45, 55, 65 and 75 days after anthesis (DAA)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abelmoschus esculentus Moench; Advanced generation lines; Characterization; Seed quality.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362010000200017

Evaluation of production and quality traits in interspecific hybrids of ornamental pepper
Provedor de dados: 75
Autores: Nascimento,Naysa Flávia F do; Rêgo,Elizanilda R do; Nascimento,Mayana F; Bruckner,Cláudio H; Finger,Fernando L; Rêgo,Mailson M do.
ABSTRACT The cultivation of potted peppers as ornamental plants has increased considerably throughout the planet. In Brazil, this crop is more recent and still lacks cultivars. In order to increase the available variability and obtain new cultivars, the interspecific hybridization is very useful in add a desirable attribute that occurs in one species to another species, resulting in a new cultivar of agronomic interest. The objective of this study was to characterize parents and interspecific hybrids based on 27 quantitative traits and evaluate their genetic diversity by multivariate procedures. Parents of seven pepper genotypes were crossed, and, seven hybrids were generated. The experiment was conducted in a completely randomized design. Data were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum spp.; Hybridization; Relative importance; Breeding; Characterization.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000300315

Variation of plant and fruit traits in native Mexican costeño pepper
Provedor de dados: 75
Autores: Santiago-Luna,Eric G; Carrillo-Rodríguez,José C; Chávez-Servia,José L; Perales-Segovia,Catarino; Heredia-Garcia,Elena.
ABSTRACT In a collection of costeño peppers conserved by indigenous producers from the municipalities of Santa Maria Tonameca and Santo Domingo de Morelos, Oaxaca, Mexico, a description and classification of agromorphological variation was undertaken by characterization of 46 populations in a greenhouse distributed under complete randomized block design with three replicates. Days to first flowering, plant and fruit traits and yield per plant were evaluated. Significant differences were detected for all traits except for plant height at 120 days after planting. In a principal component analysis, the variables of green and dry weight of 15 fruits, average fruit weight, number of fruits and yield per plant were the characteristics with major descriptive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum annuum; Phenotypic diversity; Plant genetic resources; Characterization; Multivariate analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000300399

Caracterizacao de varzeas na regiao dos cerrados.
Provedor de dados: 14
Autores: RASSINI, J. B.; REIS, A. E. G. dos; MACEDO, J.; LEITE, J. C.
A ocorrencia de varzeas na regiao dos Cerrados e grande, com cerca de 12 milhoes de hectares em sua maioria ainda nao utilizadas, devido, principalmente, a baixa disponibilidade de informacao a respeito do sistema solo-agua-planta. O trabalho busca fornecer uma caracterizao e a distribuicao desse tipo de ecossistema nas principais bacias hidrograficas dos Cerrados, apresentando resultados referentes a vegetacao e aos tipos de solo predominantes nessas formacoes.
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Flood plains; Plant soil relations; Plant water relations; Characterization; Wetland.; Bacia Hidrográfica; Cerrado; Ecossistema; Relação Solo-Planta; Relação Água-Planta; Várzea.; Ecosystems; Soil.
Ano: 1984
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/547230

Solos do Assentamento Tamarineiro II, Corumbá - MS: caracterização e potencial agrícola.
Provedor de dados: 14
Autores: CARDOSO, E. L.; OLIVEIRA, H. de; PELLEGRIN, L. A.; SPERA, S. T.; SPERA, M. R. N.
Solos do Assentamento Tamarineiro II; Caracterização e limitações agrícolas dos solos; Chernossolos; Vertissolos; Aptidão agrícola das terras; Inserção do agricultor no mercado.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Caracterização; Assentamento Tamarineiro II; Corumbá; Mato Grosso do Sul; Characterization; Agricultural suitaibility.; Aptidão Agrícola; Solo.; Soil.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/810721

Coleção de Microrganismos Patogênicos a Caprinos e Ovinos - CMPCO.
Provedor de dados: 14
Autores: FACCIOLI-MARTINS, P. Y.; SOUZA, V. de; PINHEIRO, R. R.
Resumo: Esta publicação tem por objetivo descrever a história, a composição da Coleção de trabalho de Microrganismos Patogênicos a Caprinos e Ovinos, e de que forma ela tem conservado, caracterizado, documentado e utilizado seus isolados. A coleção foi criada em 2012 e atualmente é composta por 141 cepas bacterianas e seis cepas virais, pertencentes às espécies Corynebacterium pseudotuberculosis, Staphylococcus aureus e ao gênero Lentivirus. Esses isolados são utilizados para produção de antígenos para imunodiagnósticos, em diagnósticos moleculares e na avaliação de resistência a antimicrobianos e ação de anti-sépticos e antimicrobianos. Participa de projetos das coleções institucionais e de gestão da qualidade. [Collection of Goat and Sheep Pathogenic...
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Caracterização; Germplasm bank; Characterization; Diagnosis; Quality; Pathogenic Microorganisms; Caprino; Ovino; Banco de Germoplasma; Conservação; Resistência Genética; Diagnostico; Qualidade; Sheep diseases; Goat diseases; Germplasm conservation; Conservation banks; Genetic resistance; Small ruminants; Brazil.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1123793

Solos do Assentamento 72, Ladário - MS: caracterização e potencial agrícola.
Provedor de dados: 14
Autores: CARDOSO, E. L.; OLIVEIRA, H. de; PELLEGRIN, L. A.; SPERA, S. T.; SPERA, M. R. N.
Solos do Assentamento 72; Caracterização e limitações agrícolas dos solos; Chernossolos; Gleissolos; Plintossolos; Vertissolos; Aptidão agrícola das terras; Inserção do agricultor no mercado.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Caracterização; Assentamento 72; Ladário; Mato Grosso do Sul; Characterization; Agricultural suitaibility.; Aptidão Agrícola; Solo.; Soil.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/810722

Diagnóstico da piscicultura na Bacia do Alto Taquari - MS.
Provedor de dados: 14
Autores: ROTTA, M. A.
O objetivo deste trabalho é a caracterização da situação da piscicultura na bacia do alto Taquari (BAT) a fim de se propor melhorias no seu sistema, viabilizando-o como meio alternativo de renda para os produtores da região. Este estudo foi realizado no mês de abril de 2003 através de levantamento com a aplicação de um questionário aos piscicultores da BAT. Foram obtidas informações para caracterizar os produtores, a propriedade e a produção, além de outros aspectos relevantes à atividade. Dos 24 produtores localizados na BAT, 12 (50%) se encontram em atividade, 10 estão inativos (42%) e em dois deles (8%) não foi possível obter informações. Na BAT a atividade de piscicultura vem sendo realizada principalmente em grandes propriedades, com área média de...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Caracterização; Bacia do Alto Taquari; Mato Grosso do Sul; Brasil; Diagnostic; Production; Scrutiny; Characterization; Taquari river; Wetland.; Diagnostico; Levantamento; Peixe; Produção.; Piscicultura; Brazil; Pantanal; Fish culture.; Fish.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/786918

Multiplicacao, caracterizacao e conservacao de germoplasma de tomate (Lycopersicon spp) na Embrapa Hortalicas.
Provedor de dados: 14
Autores: PESSOA, H. B. S. V.; CARVALHO, S. I. C. de.
bitstream/item/109207/1/Comunicado-tecnico-6-.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Multiplicacao; Caracterizacao; Brasilia; Distrito Federal; Brasil; Multiplication; Characterization; Conservation.; Cerrado; Conservação; Germoplasma; Lycopersicon Esculentum; Tomate.; Brazil; Germplasm; Tomatoes.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/764700

Solos do Assentamento Paiolzinho, Corumbá-MS: caracterização e potencial agrícola.
Provedor de dados: 14
Autores: CARDOSO, E. L.; OLIVEIRA, H. de; PELLEGRIN, L. A.; SPERA, S. T.; SPERA, M. R. N.
Solos do Assentamento Paiolzinho; Caracterização e limitações agrícolas dos solos; Chernossolos; Vertissolos; Aptidão agrícola das terras; Inserção do agricultor no mercado.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Caracterização; Assentamento Paiolzinho; Corumbá; Mato Grosso do Sul; Characterization; Agricultural suitaibility.; Aptidão Agrícola; Solo.; Soil.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/805595

Sterilization of Exopolysaccharides Produced by Deep-Sea Bacteria: Impact on Their Stability and Degradation
Provedor de dados: 5
Autores: Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire; Weiss, Pierre; Colliec-jouault, Sylvia.
Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared...
Tipo: Text
Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight distribution; Rheology.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf

Marine Exopolysaccharide Complexed with Scandium Aimed as Theranostic Agents
Provedor de dados: 5
Autores: Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Reiller, Pascal E.; Huclier-markai, Sandrine.
Background: Exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium, showed anti-metastatic properties. They may represent a new class of ligands to be combined with theranostic radionuclides, such as 47Sc/44Sc. The goal of this work was to investigate the feasibility of such coupling. (2) Methods: EPSs, as well as heparin used as a drug reference, were characterized in terms of molar mass and dispersity using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering (AF4-MALS). The intrinsic viscosity of EPSs at different ionic strengths were measured in order to establish the conformation. To determine the stability constants of Sc with EPS and heparin, a Free-ion selective radiotracer extraction (FISRE)...
Tipo: Text
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Characterization; Complexation.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00681/79332/81844.pdf

Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi
Provedor de dados: 5
Autores: Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques.
This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively....
Tipo: Text
Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf

Isolation and partial characterization of bacteria (Pseudoalteromonas sp.) with potential antibacterial activity from a marine costal environment from New Caledonia
Provedor de dados: 5
Autores: Dufourcq, R.; Chalkiadakis, Eletherios; Fauchon, M.; Deslandes, Eric; Kerjean, V.; Chanteau, S.; Petit, Elodie; Guezennec, Jean; Dupont-rouzeyrol, M.
Marine bacteria are a rich source of bioactive metabolites. However, the microbial diversity of marine ecosystem still needs to be explored. The aim of this study was to isolate and characterize bacteria with antimicrobial activities from various marine coastal environment of New Caledonia. We obtained 493 marine isolates from various environments and samples of which 63 (12.8%) presented an antibacterial activity against a panel of reference pathogenic strains (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis). Ten out of the most promising strains were cultured, fractionated and screened for antibacterial activity. Four of them (NC282, NC412, NC272 and NC120) showed at least an activity against reference and...
Tipo: Text
Palavras-chave: Antibacterial activity; Characterization; Marine bacteria; New Caledonia; Screening.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00174/28552/26994.pdf

Purification of the exopolysaccharide produced by Alteromonas infernus: identification of endotoxins and effective process to remove them
Provedor de dados: 5
Autores: Grivaud-le Du, Alicia; Zykwinska, Agata; Sinquin, Corinne; Ratiskol, Jacqueline; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome; Delbarre-ladrat, Christine; Colliec-jouault, Sylvia.
Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A. infernus membranes have shown the lack of the typical LPS architecture since 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo), glucosamine (GlcN), and phosphorylated monosaccharides were not present. Unlike for other...
Tipo: Text
Palavras-chave: Marine Alteromonas infernus; Bacterial exopolysaccharide; Endotoxin; Characterization; Purification; Fermentation; Process.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00397/50818/51601.pdf

Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A 2 fromBothrops atrox snake venom
Provedor de dados: 80
Autores: Menaldo,Danilo L.; Jacob-Ferreira,Anna L.; Bernardes,Carolina P.; Cintra,Adélia C. O.; Sampaio,Suely V.
Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA 2 ) from Bothrops atroxvenom, and biochemically characterize these molecules to enable future functional studies.Methods To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; Bothrops atrox; Toxins; Metalloprotease; Phospholipase A2; Isolation; Characterization; Chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100337

General characterization of venom from the Moroccan snakes Macrovipera mauritanica and Cerastes cerastes
Provedor de dados: 80
Autores: Oukkache,N; Lalaoui,M; Ghalim,N.
Ophidian envenomation accidents constitute a serious public health problem in many countries around the globe. Over 5 million such accident cases occur each year causing more than 100,000 deaths. In Africa, more than 20,000 deaths per year are registered while 400,000 envenomation victims retain severe and permanent functional sequelae. In Morocco, snakebites are frequent and of greater severity in children. They occur mostly in rural areas. The incidence of these bites remains poorly understood and vastly underestimated. The epidemiological data are not well known due to the absence of a national registry, whereas a significant proportion of envenomations receive only traditional treatment methods in non-medical intensive care. This prompted us to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerastes cerastes; Macrovipera mauritanica; Snake venom; Characterization; Biological activities; Cross-reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400009

Biometry and genetic diversity of paradise nut genotypes (Lecythidaceae)
Provedor de dados: 86
Autores: Rosa,Thuanny Lins Monteiro; Araujo,Caroline Palacio de; Alexandre,Rodrigo Sobreira; Schmildt,Edilson Romais; Lopes,José Carlos.
Abstract: The objective of this work was to evaluate the biometry of fruit and seed, and the diversity of paradise nut (Lecythis pisonis) genotypes. Fruit of 12 paradise nut genotypes were collected from plants in the northern region of the state of Espírito Santo and in the eastern region of the state of Minas Gerais, Brazil. Data were subjected to the analysis of variance, and means of the different genotypes were compared by Scott-Knott’s mean clustering test. The phenotypic diversity was analyzed using Mahalanobis’ generalized distance, and clustering, by Tocher’s optimization method and the graphical dispersion of canonical variables. The relative contribution of the characters to diversity was assessed by Singh’s method. The paradise nut genotypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lecythis pisonis; Characterization; Divergence; Genotypes; Sapucaia nut.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102502

Meloidogyne Species Associated with Weeds in Rio Grande do Sul
Provedor de dados: 87
Autores: BELLÉ,C.; KASPARY,T.E.; BALARDIN,R.R.; RAMOS,R.F.; ANTONIOLLI,Z.I.
ABSTRACT: The frequency of species of root-knot nematodes (Meloidogyne spp.) was evaluated in weeds collected in different fallow farms in the State of Rio Grande do Sul, Brazil. In the samples where the nematode was found, the species of the root-knot nematode was identified by electrophoresis using the isozyme esterase. They were obtained from weeds belonging to 24 weed species from 13 different botanical families: Amaranthaceae, Asteraceae, Commelinaceae, Convovulaceae, Cyperaceae, Euphorbiaceae, Lamiaceae, Malvaceae, Oxalidaceae, Poaceae, Portulacaceae, Solanaceae, Verbenaceae. Meloidogyne javanica Est J3 (Rm: 1.0, 1.25, 1.40) was the most frequent species and occurred in 53.3% of the samples. M. arenaria with phenotype Est. A2 (Rm: 1.20, 1.30) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Root-knot nematode; Weed plants; Isozyme esterase; Characterization; Hostability.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100292

PLANTAS MEDICINAIS COMO ALTERNATIVA DE NEGÓCIOS: CARACTERIZAÇÃO E IMPORTÂNCIA
Provedor de dados: 31
Autores: Correa,   Cynthia Candida; Alves,   Alexandre Florindo.
O uso dos produtos à base de plantas medicinais surge como uma forte tendência mundial. Esse é considerado um mercado promissor e apresenta números expressivos, tornando-se um ambiente de negócio atrativo. Entretanto, apesar de toda importância social e econômica, o Brasil e o Paraná não tem investido adequadamente na produção, industrialização e distribuição dessas plantas. Nesse contexto, buscou-se, através desse trabalho, caracterizar e analisar a produção de plantas medicinais no estado do Paraná. Para alcance desse objetivo foram utilizados dados secundários, encontrados em livros, artigos científicos, revistas e, principalmente, em sites governamentais. Pode-se concluir, a partir da pesquisa, que existe um aumento da oferta e demanda de plantas...
Tipo: Conference Paper or Presentation
Palavras-chave: Plantas medicinais; Sistema produtivo; Caracterização; Paraná; Medicinal plants; Productive system; Characterization; Paraná state - Brazil; Health Economics and Policy.
Ano: 2008
URL: http://purl.umn.edu/108079

ANÁLISE DA CADEIA PRODUTIVA DA CAPRINO-OVINOCULTURA EM GARANHUNS
Provedor de dados: 31
Autores: Carvalho,   Daniela Moreira de; Souza,   Jalmir Pinheiro De.
A caprino-ovinocultura na região Nordeste tem grande importância tanto econômica quanto social, devido ao fato dos animais terem uma boa adaptação ao clima e a alimentação, muitas vezes escassa, disponível na região. A compreensão das cadeias produtivas locais permite a proposição de melhorias no setor a fim de torná-lo mais eficiente. Na caprino-ovinocultura a eficiência ocorre através da oferta aos consumidores de carnes de melhor qualidade, animais jovens, constância de oferta, segurança alimentar e preços competitivos. O resultado da eficiência do sistema produtivo seria de uma maior rentabilidade, uma inserção em novos mercados, ou seja, numa maior competitividade de todo setor. Nesse sentido objetivou-se realizar uma análise descritiva da cadeia...
Tipo: Conference Paper or Presentation
Palavras-chave: Caprino-ovinocultura; Cadeia produtiva; Coordenação; Caracterização; Garanhuns; Sheep &amp; goat farming; Productive chain; Coordination; Characterization; Garanhuns; Livestock Production/Industries.
Ano: 2008
URL: http://purl.umn.edu/109705

DESENVOLVIMENTO SUSTENTÁVEL E SOJICULTURA EM RORAIMA: TRAJETÓRIAS ANTAGÔNICAS OU CONCILIÁVEIS?
Provedor de dados: 31
Autores: Gianluppi,   Luciana Dal; Waquil,   Paulo Dabdab.
Quando há o entendimento de que crescimento e desenvolvimento são sinônimos, tende-se a prezar uma dimensão do desenvolvimento em detrimento das outras, o que acaba por prejudicar a população por meio de carências nas condições de vida e destruição do meio ambiente principalmente. O ideal do desenvolvimento sustentável surge na tentativa de harmonizar crescimento econômico, igualdade social e preservação ambiental. Buscando avaliar se existe compatibilidade entre essas três dimensões que o problema dessa pesquisa foi proposto – a expansão da sojicultura em Roraima é sustentável? –, uma vez que, na expansão da produção de soja pelo Brasil, as dimensões econômica, social e ambiental nem sempre caminham juntas. Para alcançar o objetivo de observar a...
Tipo: Conference Paper or Presentation
Palavras-chave: Sojicultura; Desenvolvimento sustentável; Roraima; Caracterização; Índices; Soybean production; Sustainable development; Roraima; Characterization; Indexes; International Development.
Ano: 2008
URL: http://purl.umn.edu/109116

Caracterización de las ferias libres como canal de comercialización en la Región Metropolitana de Chile
Provedor de dados: 31
Autores: Saez,   Luis; Torres,   Victoria; Caceres,   Leonardo.
Published by Asociación de Economistas Agrarios de Chile
Tipo: Journal Article
Palavras-chave: Buying behaviour; Characterization; Fresh vegetable and fruit street fairs market channel.; Agribusiness; Marketing.
Ano: 2010
URL: http://purl.umn.edu/104271

Workshop Report: Advancing the Art of Analyzing Risks and Benefits of Dredged Material Management
Provedor de dados: 31
Autores: Wilson,   James D.
The U.S. Army Corps of Engineers has been delegated responsibility for assuring the navigability of waters designated for that purpose in the United States. Dredging is among the activities required to fulfill this responsibility. In some places, the Corps finds difficulty in carrying out dredging projects because it lacks spaces where the sediments that must be removed ("dredged materials") can be placed or otherwise managed. In these cases, the public has expressed concerns about the impacts of dredged materials on the environment, either underwater or on land ("upland"). Thus, the Corps often finds it necessary to be able to evaluate and describe the costs or risks and the benefits of the possible alternatives that may be available. Scientists and...
Tipo: Working or Discussion Paper
Palavras-chave: Dredging; Risk; Benefit; Cost; Analysis; Characterization; Accidents; Mixtures; Sediments; Non-use benefits; Decision tools; Environmental Economics and Policy.
Ano: 2002
URL: http://purl.umn.edu/10617

CARACTERIZAÇÃO DA CADEIA PRODUTIVA DA CARNE BOVINA NO ESTADO DE SÃO PAULO
Provedor de dados: 31
Autores: Tirado,   Geovana; Costa,   Sergio Jose; Bliska,   Flavia Maria De Mello; Igreja,   Abel Ciro Minniti.
O trabalho de pesquisa analisou a cadeia produtiva da carne bovina (CPCB) no Estado de São Paulo, com objetivo geral de diagnosticar e caracterizar a CPCB no Estado de São Paulo, com a finalidade de detectar os pontos fracos que prejudicam a sua competitividade, e com objetivos específicos de caracterizar a CPBC no Estado de São Paulo, identificar os principais sistemas de produção pecuária predominantes e elaborar um modelo da CPCB para o estado. A coleta dos dados secundários se balizou na sistematização de um conjunto de informações sobre a CPCB no Brasil e no Estado de São Paulo. E a coleta dos dados primários foi feita mediante realização de entrevistas com informantes-chave dos elos dessa cadeia, aplicando questionários semi-estruturados. A CPCB...
Tipo: Conference Paper or Presentation
Palavras-chave: Cadeia produtiva da carne bovina; Caracterização; Estado de São Paulo; Beef cattle production chain; Characterization; State of São Paulo; Livestock Production/Industries.
Ano: 2008
URL: http://purl.umn.edu/110001

Caracterización de los pequeños ganaderos ovinos del secano costero de la Región de O’Higgins para su potencial inserción en la cadena exportadora de carne ovina
Provedor de dados: 31
Autores: Saez,   Luis; Caceres,   Leonardo; Leporati,   Michel.
Published by Asociación de Economistas Agrarios de Chile
Tipo: Journal Article
Palavras-chave: Characterization; Quality; Small-livestock producers; Agribusiness; Livestock Production/Industries.
Ano: 2008
URL: http://purl.umn.edu/97371

Genetic divergence of Anthurium affine germplasm using morphoagronomic and molecular descriptors
Provedor de dados: 124
Autores: Maia,Clara Yohana; Soares,Nazaré Suziane; Castro,Ana Cecília Ribeiro; Queirós,João Ravelly Alves de; Aragão,Fernando Antonio Souza de; Bordallo,Patricia do Nascimento.
ABSTRACT Anthurium affine is an ornamental Brazilian native plant very appreciated due to its long-lasting durability. To explore the genetic diversity available, 21 A. affine half-sibling progeny were evaluated using morphoagronomic and molecular descriptors. The morphoagronomic descriptors used were: plant height, plant diameter, growth habit, leaf number, leaf blade length, leaf blade width, petiole length, petiole diameter, leaf margin undulation, general appearance; soil cover rate and Random Amplified Polymorphic DNA (RAPD) markers the molecular one. All the descriptors within the progeny showed variability and general appearance, leaf margin undulation and soil cover rate are the ones that most contribute to the plants divergence. From 45 RAPD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clustering; Characterization; Genetic variation; Ornamental; RAPD.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400410

Variability in ribosomal DNA genic and spacer regions in Verticillium dahliae isolates from different hosts
Provedor de dados: 102
Autores: Otero,Laura; Ducasse,Daniel; Miller,Robert N. G.
Using PCR-based assays with specific primers for amplification of the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates from olive (Olea europaea) and other host species from Argentina and Brazil was estimated. The derived UPGMA-generated phenograms based upon the restriction fingerprinting data of rDNA IGS products revealed genetic differences, correlating with the host of origin. Isolates infecting olive genetically distinct from those from cocoa (Theobroma cacao) and sunflower (Helianthus annuus). Digestion of mitochondrial DNA SSU rRNA PCR products revealed less variability, distinguishing only one isolate...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Olea europaea; Molecular markers; Characterization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000400015

New human case reports of cutaneous leishmaniasis by Leishmania (Viannia) naiffi in the Amazon region, Brazil
Provedor de dados: 36
Autores: FIGUEIRA,Luanda de Paula; SOARES,Fabiane Veloso; JÚNIOR,Roberto Daibes NAIFF; VINHOTE-SILVA,Alana Cristina; SILVA,Susi Simas da; ESPIR,Thaís Tibery; NAIFF,Maricleide de Farias; GOMES,Luis Henrique Monteiro; MOREIRA,Flávia Regina Almeida Campos Naief; FRANCO,Antonia Maria Ramos.
ABSTRACT Few cases of human cutaneous leishmaniasis (CL) caused by Leishmania naiffi were described in the medical literature. The aim of this study was to report and analyze new cases of L. naiffi in the period between the years 1992 to 2011. The strains were characterized by isoenzyme analysis. All patients assisted had small lesions; ranging from 1.0x1.0 mm and 13.5x11.5 mm. The lesions observed were widely distributed: 55.5% on the lower limb, 5.5% in the abdominal area, 16.6% on upper limb and 22.2% in upper limb and back. Seventy-two percent of patients had ulcerated lesions. Clinical course of the disease varied from 1 to 10 months. According to gender, most infected individuals were men (83.3%). The patients came from Amazonas (10), Pará (01) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: American Tegumentary Leishmaniasis; Leishmania naiffi; Characterization; Experimental stations; Lutzomyia.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000100047

Caracterização morfológica de plantas de rambutan - DOI: 10.4025/actasciagron.v31i4.890
Provedor de dados: 1
Autores: Andrade, Renata Aparecida de; UNESP - Jaboticabal/SP; Lemos, Eliana Gertrudes de Macedo; UNESP - Jaboticabal/SP; Martins, Antonio Baldo Geraldo; UNESP - Jaboticabal/SP; Paula, Rinaldo César de; UNESP - Jaboticabal/SP.
Em certas espécies vegetais, a distinção entre variedades pode ser realizada com base em aspectos morfológicos das plantas, o que permite a identificação mesmo quando não apresentam flores e/ou frutos. Diante disso, realizou-se o presente trabalho cujo objetivo foi a caracterização morfológica e o agrupamento de plantas de rambutan. Foram avaliados: altura da planta (cm), diâmetro e geometria da copa (entre linhas e entre plantas, em cm), diâmetro do tronco (cm) e disposição dos ramos no tronco, tamanho de folhas e folíolos (comprimento e largura, em cm), número de folíolos, comprimento da ráquis (cm), comprimento do pecíolo (cm), área foliar (cm2), vigor das plantas, sensibilidade ao frio, formato dos folíolos, pilosidade, coloração dos folíolos (faces...
Palavras-chave: 5.01.03.04-0    Produção de Mudas Nephelium lappaceum; Morfologia; Caracterização fitotecnia Nephelium lappaceum; Morphology; Characterization.
Ano: 2009
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/890

Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis - doi: 10.4025/actascibiolsci.v32i4.6276
Provedor de dados: 3
Autores: Costa, Geraldo Márcio da; UFLA; Paiva, Luciano Vilela; UFLA; Piccoli, Roberta Hilsdorf; Universidade Federal de Lavras; Figueiredo, Demétrio Junqueira; Universidade Federal de Lavras; Pereira, Ulisses de Pádua; UFLA; Silva, Nivaldo da; EV-UFMG.
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit...
Tipo: Pesquisa laboratorial
Palavras-chave: 5.05.02.03-4 mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests Doenças Infecciosas dos Animais mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276

Influence of environment on the leaf morpho-anatomy and histochemical of the ayahuasca leaf: Populations cultivated in extra-Amazonian regions
Provedor de dados: 3
Autores: Miranda, Ordilena Ferreira de; de Souza, Saulo Eduardo Xavier Franco; Milan, Rodrigo José; Bueno, Aline Borges; Almeida, Marcilio de.
Psychotria viridis Ruiz &amp;amp; Pav. (Rubiaceae) occurs naturally throughout the Amazon and it is traditionally used by indigenous communities, being incorporated into religious use in urban contexts over the last few decades. It is known and cultivated in many regions of South America for possessing valuable bioactive alkaloids. In this paper, we described P. viridis leaf morphology, anatomy and histochemistry from three populations cultivated in the southeastern Brazil, in order to identify possible adaptations to local environment and management. All plants presented terminal stipules and basic morpho-anatomical patterns of leaves, consistent with most species of the genus, as heterogeneous dorsiventral mesophyll, uniseriate epidermis, presents large...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Alkaloid; Trichomes; Glandular duct; Domatia. characterization; Alkaloid; Trichomes; Secretory duct; Domatia.
Ano: 2020
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/50369

Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
Provedor de dados: 3
Autores: Peixoto, Ferdyanne Beatriz Sabóia; Peixoto, Jean Charles da Cunha; Assunção, Enedina Nogueira de; Peixoto, Elise Miranda; Pereira, José Odair; Astolfi-Filho, Spartaco.
Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiologia 2.12.00.00-9 biodegradation; Characterization; DCPIP; Antimicrobial resistance. Microbiologia.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223

PRODUCTION AND CHARACTERIZATION OF BIOFUEL PRODUCTION FROM EGUSI (CITRULLUS LANATUS) VEGETABLE OIL
Provedor de dados: 111
Autores: I., Manta H.; D., James D.; I., Awu J.
Green house effect and ozone layer depletion due continuous environmental pollution from fossil fuel has speed up the quest for search of alternative energy fuel. In recent year, biofouls has attracted much attention as a reliable substitute to fossil fuel. The objective of this study was to evaluate and characterize biofuel produced from Egusi vegetable oil by applying standard method of transesterification. This study was carried out at Processing and Storage Engineering Departmental (PSED) laboratory of National Centre for Agricultural Mechanization (NCAM), Ilorin Kwara State, Nigeria. The results of the proximate characterization of the produced Egusi vegetable oil shows crude protein, crude fibre, ether extract, moisture content, ash content and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Egusi Vegetable Oil; Characterization; Transesterification; Methyl Esters; Biofuel.
Ano: 2021
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/6205

Characterization of Capsicum germplasm collected in Northwestern Argentina based on morphological and quality traits
Provedor de dados: 114
Autores: Occhiuto,P.N; Peralta,I.E; Asprelli,P.D; Galmarini,C.R.
Paprika production is concentrated in Northwestern Argentina, where around 1500 ha are annually grown. Farmers mainly from Andean valleys, maintain their local landraces or &amp;#8220;criollas&amp;#8221; under different environmental conditions, using their farming traditions and selection criteria. These traditional varieties represent valuable genetic resources for conservation and selection of agronomical and quality traits. Between 2005 and 2007 eight collecting trips were made to Northwestern Argentina in order to recover local germplasm. As a result, five hundred new vegetable seed samples have been incorporated into the Germplasm Bank. Among these accessions 32 belong to Capsicum, the majority for paprika production, that were evaluated in field...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum annuum; C. baccatum; Germplasm; Characterization; Pungency; Pre-breeding.
Ano: 2014
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2014000200002

Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw09
Provedor de dados: 42
Autores: MA,YUPING; SUN,SIWEN; HAO,HUI; XU,CHUNPING.
ABSTRACT A soil isolate, Penicillium janthinellum sw09 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as exo-polygalacturonase (exo-PG). By optimizing growth conditions, P. janthinellum sw09 produced high amount of exo-PG (16.54 units/mL). The crude enzyme was purified by gel filtration chromatography and two exo-PG activity peaks (designated as PGI and PGII) were revealed. On SDS-PAGE analysis, purified PGII using DEAE-Sepharose FF column, was found to be a single band with a molecular mass of 66.2 kDa. The purified PGII exhibited maximal activity at the temperature of 45 oC and pH 5.0. The stability profiles show that PGII is more stable in the pH range of 4.0-8.0 and below 60 oC. The Km and Vmax...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium janthinellum; Polygalacturonase; Purification; Characterization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200479

The effect of precursors salts on surface state of Pd/Al2O3 and Pd/CeO2/Al2O3 catalysts
Provedor de dados: 42
Autores: Guimarães,André L.; Dieguez,Lídia C.; Schmal,Martin.
The influence of the precursors on the promoting effect of ceria on Pd/Al2O3 catalyst, when ceria is coated over alumina was studied. The reaction of propane oxidation proceeded under different feed conditions and the surface active sites were characterized by X-ray photoelectron spectroscopy (XPS) and in situ diffuse reflectance spectroscopy (DRS). XPS and DRS results show that PdO/Pd0 interface are the active sites independent of the precursor, while the catalysts containing CeO2 showed formation of palladium species in the highest oxidation state, probably PdO2 (338 eV) after the oxidation of propane. Besides, the O/Al and O/Ce ratios evidenced the increase of oxygen storage in the presence of CeO2. In addition, the precursor acetylacetonate favors the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Palladium; Cerium oxide; Precursors; Characterization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400014

Characterization of granite and limestone powders for use as fillers in bituminous mastics dosage
Provedor de dados: 42
Autores: BARRA,BRENO; MOMM,LETO; GUERRERO,YADER; BERNUCCI,LIEDI.
This paper discusses the importance of studies on materials known as fillers from different mineral origins, used in asphalt mixes, specifically in the formulation of mastics. The research was carried out on samples of limestone and granite rock filler and asphalt binder (50/70). The samples were evaluated through semiquantitative chemical analyses by X-ray fluorescence, granulometry by low angle laser emission, scanning electron microscopy, softening point tests, penetration tests, and aggregate-asphalt binder and aggregate-mastic adhesion tests. The results highlighted convergent trends, indicating that the active behavior of the fillers in the mastic formulation is not related to the size of the particles, but rather to their form, surface texture,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Fillers; Granite; Limestone; Bituminous mastics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200995

Towards an atomic level understanding of niobia based catalysts and catalysis by combining the science of catalysis with surface science
Provedor de dados: 42
Autores: Schmal,Martin; Freund,Hans-Joachim.
The science of catalysis and surface science have developed, independently, key information for understanding catalytic processes. One might argue: is there anything fundamental to be discovered through the interplay between catalysis and surface science? Real catalysts of monometallic and bimetallic Co/Nb2O5 and Pd-Co/Nb2O5 catalysts showed interesting selectivity results on the Fischer-Tropsch synthesis (Noronha et al. 1996, Rosenir et al. 1993). The presence of a noble metal increased the C+5 selectivity and decreased the methane formation depending of the reduction temperature. Model catalyst of Co-Pd supported on niobia and alumina were prepared and characterized at the atomic level, thus forming the basis for a comparison with "real" support...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalysis; Model catalysts; Cobalt; Palladium; Characterization; Niobia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000200016

A weighted negative binomial Lindley distribution with applications to dispersed data
Provedor de dados: 42
Autores: BAKOUCH,HASSAN S.
Abstract A new discrete distribution is introduced. The distribution involves the negative binomial and size biased negative binomial distributions as sub-models among others and it is a weighted version of the two parameter discrete Lindley distribution. The distribution has various interesting properties, such as bathtub shape hazard function along with increasing/decreasing hazard rate, positive skewness, symmetric behavior, and over- and under-dispersion. Moreover, it is self decomposable and infinitely divisible, which makes the proposed distribution well suited for count data modeling. Other properties are investigated, including probability generating function, ordinary moments, factorial moments, negative moments and characterization. Estimation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Discrete distributions; Estimation; Vuong test statistic; Mixture distributions; Thunderstorms data.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602617

A Novel Porous Diamond - Titanium Biomaterial: Structure, Microstructure, Physico-Mechanical Properties and Biocompatibility
Provedor de dados: 42
Autores: GUIMARÃES,ZULMIRA A.S.; DAMATTA,RENATO A.; GUIMARÃES,RENAN S.; FILGUEIRA,MARCELLO.
ABSTRACT With the aim of introducing permanent prostheses with main properties equivalent to cortical human bone, Ti-diamond composites were processed through powder metallurgy. Grade 1 titanium and mixtures of Ti powder with 2%, 5% and 10 wt% diamond were compacted at 100MPa, and then sintered at 1250°C/2hr/10-6mbar. Sintered samples were studied in the point of view of their microstructures, structures, yield strength and elastic modulus. The results showed that the best addition of diamonds was 2 wt%, which led to a uniform porosity, yield strength of 370MPa and elastic modulus of 13.9 GPa. Samples of Ti and Ti-2% diamond were subjected to in vitro cytotoxicity test, using cultures of VERO cells, and it resulted in a biocompatible and nontoxic composite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomaterials; Titanium; Diamond; Sintering; Characterization; Biocompatibility.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000603111

Detection, purification and characterization of a lectin from freshwater green algae Spirogyra spp.
Provedor de dados: 42
Autores: OLIVEIRA,ANTÔNIA S. DE; LÓSSIO,CLÁUDIA F.; RANGEL,ANNE J.; MARTINS,MARIA G.Q.; NASCIMENTO,FERNANDO E.P. DO; ANDRADE,MARIA L.L. DE; CAVADA,BENILDO S.; LACERDA,SÍRLEIS R.; NASCIMENTO,KYRIA S. DO.
ABSTRACT Freshwater algae are rich sources of structurally biologically active metabolites, such as fatty acids, steroids, carotenoids and polysaccharides. Among these metabolites, lectins stand out. Lectins are proteins or glycoproteins of non-immune origin which bind to carbohydrates or glycoconjugates, without changing ligand structure. Many studies have reported on the use of Spirogyra spp. as effective bioindicators of heavy metals; however, reports on Spirogyra molecular bioprospecting are quite limited. Therefore, this study aimed to detect, isolate, purify and characterize a lectin present in the freshwater green algae Spirogyra. Presence of the lectin protein in the extract was detected by hemagglutination assays. Subsequently, the protein extract...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Algae; Characterization; Lectin; Purification.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502113

Isolation and genetic stability of an infectious bronchitis virus strain (IBV)
Provedor de dados: 45
Autores: Pereira,C.G.; Saraiva,G.L.; Santos,M.R.; Assao,V.S.; Fietto,J.L.R.; Bressan,G.C.; Almeida,M.R.; Moreira,M.A.S.; Silva Júnior,A.
RESUMO O vírus da bronquite infecciosa (IBV) é um importante patógeno respiratório presente na avicultura comercial e tem provocado grandes perdas econômicas em todo o mundo. A vacinação é realizada pela indústria produtora de aves, mas continuam surgindo novos sorotipos e variações antigênicas, dificultando o controle de IBV. Nós realizamos uma caracterização molecular de uma cepa de IBV obtida diretamente de tecidos e comparamos com a mesma cepa que havia sido passada três vezes em ovo embrionado. Nós mostramos uma variação significante na sequência viral depois de ter sido isolada em ovo embrionado.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Infectious bronchitis virus; Isolation; Characterization; Stability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401333

Some Biochemical properties of polyphenoloxidase from spearmint (Mentha arvensis)
Provedor de dados: 52
Autores: Neves,Valdir Augusto; Picchi,Douglas Gatte; Silva,Maraiza Aparecida da.
Polyphenoloxidase (PPO; EC 1.14.18.1) extracted from Mentha arvensis leaves was isolated by (NH4)2SO4 precipitation and extensive dialysis. Its optimum pH and temperature varied with the substrate. The PPO showed activity with various diphenols. Km values were found 0.825, 0.928 and 7.41mM for caffeic acid, 4-methylcatechol and catechol, respectively. On heat-inactivation, half of the activity was lost after 60 and 15 sec at 70 and 75ºC, respectively. Measuring of residual activity showed a stabilizing effect of sucrose at various temperatures with activation energy (Ea) for inactivation increasing with sucrose concentration from 0 to 40% (w/w). Ea values of 78.13; 80.37; 82.79 and 81.00 kJ/Mol were found for 0, 15; 30 and 40% sucrose, respectively. PPO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyphenoloxidase; Mentha arvensis; Characterization; Heat inactivation; Inhibitors.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400025

Characterization of the penicillin G acylase from Bacillus megaterium ATCC 14945
Provedor de dados: 52
Autores: Souza,Vanessa Ribeiro de; Silva,Ana C. G.; Pinotti,Laura Marina; Araújo,Heloísa Sobreiro Selistre; Giordano,Raquel de Lima Camargo.
The purpose of this work was to characterize the enzyme penicillin G acylase (PGA) produced by Bacillus megaterium. Purification of the enzyme by ultra/diafiltration did not allow the detection of the PGA band by SDS-PAGE electrophoresis due to the high content of remaining proteins. However, using the DNA of the microorganism, it was possible to replicate the genes of the two B. megaterium PGA reported in literature, showing that the enzyme consisted of two sub-units, having 245 and 537 amino acids each and an average molecular mass of 26950 and 59070 Da, respectively. The parameters studied were: 1) the influence of temperature in the 25-60(0)C range, 2) pH in the 5-10 range and 3) substrate concentration, this was tested to obtain results on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillin G acylase; Bacillus megaterium; Characterization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400013

Extraction of lipase from Burkholderia cepacia by PEG/Phosphate ATPS and its biochemical characterization
Provedor de dados: 52
Autores: Padilha,Giovana da Silva; Santana,José Carlos Curvelo; Alegre,Ranulfo Monte; Tambourgi,Elias Basile.
This work aimed to study the partitioning of a lipase produced by Burkholderia cepacia in PEG/Phosphate aqueous two phase system (ATPS) and its characterization. Lipase was produced by B. cepacia strains in a fermenter. Enzyme partitioning occurred at pH 6.0 and 8.0, using PEG 1500 and 6000 on two tie lines. Metal ions, pH and temperature effects on enzyme activity were evaluated. Five milliliter of 7.5% olive oil emulsion with 2.5% gumarabic in 0.1M sodium phosphate buffer at pH 8.0 and 37ºC were used for the activity determinations. Results showed that crude stratum from B. cepacia was partitioned by PEG1500/phosphate ATPS at pH 6.0 or 8.0 for, which the partitioning coefficients were 108-and 209-folds. Lipase presented optimal activity conditions at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipase; Burkholderia cepacia; Aqueous two phase system; Partitioning; Characterization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100002

The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS
Provedor de dados: 52
Autores: Ferreira,Juliana Ferrari; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromelain; Aqueous two-phase systems; Partitioning; Characterization; Pineapple.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100017

Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae
Provedor de dados: 52
Autores: Mushtaq,Zareena; Irfan,Muhammad; Nadeem,Muhammad; Naz,Mammona; Syed,Quratulain.
In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35ºC for 168 h of fermentation. Alkaline protease was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of alkaline protease was 69 kDa determined by 10% SDS-PAGE. The optimum pH and temperature of alkaline protease was 9.0 and 40ºC, respectively. Metal profile of the enzyme showed that the enzyme was non-metallic in nature. The Km ,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinetics; Purification; Characterization; Protease; Detergent stability; Rhizopus oryzae.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200175

Isolation and Characterization of Midgut Lectin From Aedes aegypti (L.) (Diptera: Culicidae)
Provedor de dados: 52
Autores: Ayaad,Tahany Hassan; Al-Akeel,Rasha Khalifah; Olayan,Ebtisam.
ABSTRACT The present investigation deals with the isolation and characterization of a lectin from Aedes aegypti (Ae aegypti) female mid gut extract that agglutinates various mammalian red blood cells (RBCs) such as human three groups A, B, and O (RH+), mouse, rat, guinea-pig, sheep and goat erythrocytes. The highest activity of both crude and isolated mid gut lectins were detected against sheep RBCs. Using (NH4)2 SO4 fractionation, ion-exchange and mannose-CNBr-Sepharose 6B affinity chromatography techniques, Ae. aegypti midgut lectin (Aelec) was purified to homogeneity.Isoelectric focusing (IEF) and reducing SDS/PAGE revealed that the isolated mid gut lectin had isoelectric point (PI) of 5.90, and subunits approximate molecular weights of 35.50 and 27.35...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lectin; Purification; Characterization; Hemagglutination; Aedes aegypti; Mosquitoes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600905

Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation
Provedor de dados: 52
Autores: Benjamin,Sailas; Pandey,Ashok.
Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40°C and pH 7-8....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200016

Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes
Provedor de dados: 52
Autores: Duarte,Ana Rodrigues; Duarte,Débora Maria Rodrigues; Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Lima-Filho,José Luiz de; Porto,Ana Lúcia Figueiredo.
The partial characterization and purification of milk clotting enzyme obtained from the (root latex) of Jacaratia corumbensis O. kuntze was studied, by fractional precipitation with ammonium sulphate and ion exchange chromatography. The ammonium sulphate precipitate showed five fractions (AS1- 0-20%; AS2 - 20-40%; AS3 - 40-60%; AS4 - 60-80%; AS5 - 80-100%) and among the fractions obtained, the 40-60% fraction (AS3) showed the highest milk clotting activity with a purification factor of 1.2 fold in relation to the crude extract. This fraction when applied on Mono Q column yielded two protein peaks (p1 and p2), but p1 pool showed the best milk-clotting activity. The optimal pH for the crude and partially purified extract was 6.5 and 7.0, respectively. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jacaratia corumbensis O. kuntze; Milk clotting enzyme; Characterization; Purification; Vegetable enzyme.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100001

Production and Optimization of Killer Toxin in Debaryomyces hansenii Strains
Provedor de dados: 52
Autores: Çorbacı,Cengiz; Uçar,Füsun Bahriye.
ABSTRACT Postharvest diseases of fruits and vegetables result in critical losses of production in worldwide. The losses often are caused by fungi and nowadays, most fungal pathogens are controlled by several strategies such as the use of fungicides. However, most of the fungicides are chemical-based compounds and are dangerous to human health and the nature. Therefore, the discovery of healthy and reliable strategies is crucial to control of fungal pathogens. In the paper, it was aimed to evaluate and characterize yeast isolates previously isolated from dairy products for the production of killer toxin. A total of 18 yeasts have been found to produce antagonistic behavior against susceptible fungal species. All of the yeasts expressing killer character...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Killer toxin; Optimization; Postharvest disease; Yeast.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100402

Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation
Provedor de dados: 52
Autores: Benjamin,Sailas; Pandey,Ashok.
Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40ºC and pH 7-8....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500002

Characterization Of A Novel Hydrolytic Enzyme Producing Thermophilic Bacterium Isolated From The Hot Spring Of Azad Kashmir-Pakistan
Provedor de dados: 52
Autores: Zahoor,Sana; Javed,Muhammad Mohsin; Babarl,Masroor Ellahi.
ABSTRACT A thermophilic bacterium (TP-2) was isolated from the Tatta Pani hot spring in Azad Kashmir and was characterized using phenotypic and genotypic characters. The strain developed cream colored, round, smooth, flat and slimy colonies while the cells were Gram positive rods that ranged in size from about 2.1-3.6 μm to 0.2-0.3 μm in width. Sequence analysis of its 16S rRNA gene showed that isolate TP-2 had 89% homology with Geobacillus debilis. It grew within pH range of 5.5 to 8.5 with optimum growth at pH 7.0. The isolate showed optimum growth at 65ºC and gave positive results for gelatin hydrolysis (GEL), ortho nitrophenyl-β-D-galactopyranosidase (ONPG), and nitrate production and produced acid from sucrose, glucose and maltose. It utilized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tatta Pani; Characterization; Enzyme production; Geobacillus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100338

Isolation of alkalophilic CGTase-producing bacteria and characterization of cyclodextrin-glycosyltransferase
Provedor de dados: 52
Autores: Higuti,lma Hiroko; Grande,Simone Wichert; Sacco,Roberta; Nascimento,Aguinaldo José do.
One hundred and twenty five soil samples were collected from the regions of roots of corn, cassava, potato, bean, sugar cane, soya, and pumpkin. From these, 75 strains were isolated that produced a yellowish halo surrounding the colonies, due to a phenolphtalein-cyclodextrin (CD) complex, and these were selected as alkalophilic CGTase-producing bacteria. All the 75 strains were identified as Bacillus firmus by microscopy and biochemical tests. The activity of the CGTase's varied from 2² to 2(10) dilutions,when assayed by CD-trichloroethylene (TCE)-complex precipitation. Strain 31 that produced the enzyme at the higher level was selected, and its enzyme was partially purified by starch adsorption (x 17) in a yield of 51%. Maximum enzyme activity occurred at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Characterization.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200007

Microbial Synthesis and Characterization of Biodegradable Polyester Copolymers from Burkholderia Cepacia and Cupriavidus Necator Strains Using Crude Glycerol as Substrate
Provedor de dados: 52
Autores: Rodrigues,Plínio Ribeiro; Silvério,Tatiane Aparecida Barroso; Druzian,Janice Izabel.
Abstract Polymers are materials of pronounced importance in the modern world, since they are massively present in everyday life, especially in the form of packaging. However, most of these materials are derived from non-renewable sources and their disposal generates large volumes of waste, which is extremely damaging to natural environments. In this context, microbial biopolymers appear as a powerful alternative in the substitution of several applications of synthetic plastics, causing less harm to the environment, as they are biodegradable and produced from renewable sources. In this study, evaluation and characterization of polyhydroxyalkanoates (PHAs) produced by Cupriavidus necator (IPT 026 and IPT 027) and Burkholderia cepacia (IPT 119 and IPT 400),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crude glycerol; Biosynthesis; Polyhydroxyalkanoates; Characterization.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100209

Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus
Provedor de dados: 52
Autores: Martos,María Alicia; Butiuk,Ana Paula; Rojas,Natalia Lorena; Hours,Roque Alberto.
The aim of this work was to study the purification and physicochemical properties of an endo-polygalacturonase (PG) produced by Wickerhamomyces anomalus isolated from the citrus fruit peels. The enzyme was purified to homogeneity from the culture filtrate of W. anomalus grown on the yeast nitrogen base medium with glucose as carbon and energy source and citrus pectin as inductor. After anion-exchange chromatography and gel filtration chromatography, PG activity was eluted as a single peak, yielding 21% of the original activity. After dialysis and cation-exchange chromatography, only one fraction with PG activity was obtained, recovering 56% of initial enzyme activity and 1.3-fold increase in specific activity. The molecular weight of the enzyme was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wickerhamomyces anomalus; Polygalacturonase; Purification; Characterization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400587

Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil
Provedor de dados: 55
Autores: Lancellotti,Marcelo; Pace,Fernanda de; Stehling,Eliana Guedes; Villares,Maria Cecília Barisson; Brocchi,Marcelo; Silveira,Wanderley Dias da.
Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemophilus influenzae; Characterization; Ribotyping; Serotype; Biotype.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500015

Characterization of bovine respiratory syncytial virus isolated in Brazil
Provedor de dados: 56
Autores: Arns,C.W.; Campalans,J.; Costa,S.C.B.; Domingues,H.G.; D'Arce,R.C.F.; Almeida,R.S.; Coswig,L.T.
This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and non-hemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine respiratory syncytial virus; BRSV; Virus isolation; Characterization; Nested RT-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200008

Characterization and comparison of Serratia marcescens isolated from edible cactus and from silkworm for virulence potential and chitosan susceptibility
Provedor de dados: 58
Autores: Li,Bin; Yu,Rongrong; Liu,Baoping; Tang,Qiaomei; Zhang,Guoqing; Wang,Yanli; Xie,Guanlin; Sun,Guochang.
Representative strains of Serratia marcescens from an edible cactus plant and silkworms were characterized and a comparison based on their cellular fatty acid composition, 16S rRNA and groE gene sequence analysis as well as silkworm virulence and chitosan susceptibility was carried out. Results from this study indicate that there are no significant differences between the phenotypic and molecular characterization, virulence and chitosan susceptibility of the S. marcescens strains from the cactus plant and silkworms. Silkworms inoculated with S. marcescens from either plant or silkworm resulted in nearly 100% mortality. Chitosan solution exhibited strong antibacterial activity against S. marcescens. This activity increased with the increase of chitosan...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serratia marcescens; Cactus; Silkworm; Characterization; Virulence; Chitosan susceptibility.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100013

Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
Provedor de dados: 58
Autores: Angelini,Michelle; Stehling,Eliana Guedes; Moretti,Maria Luiza; Silveira,Wanderley Dias da.
Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shigella sonnei; Shigella flexneri; Epidemiology; Characterization; PFGE.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034

Characterization of recombinant MSP5 Anaplasma marginale Havana isolate
Provedor de dados: 58
Autores: Corona,B.; Machado,H.; Rodríguez,M.; Martínez,S.
Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic characterization of A. marginale strains and phylogenetic studies. The major surface protein 5 (MSP5) is highly conserved in the genus Anaplasma and in all isolates of A. marginale. The aim of the present work was to carry out the cloning, sequencing and characterization of the recombinant MSP5 Anaplasma marginale Havana isolate. The sequence of the msp5 gene of Anaplasma marginale Havana isolate with a size of 633 pb was determined (Acc. No. AY527217). This gene was cloned into pRSETB vector and expressed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaplasma marginale; Major surface proteins; Characterization; Havana isolate; HMSP5.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400032

Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
Provedor de dados: 58
Autores: Li,Jiang; Hu,Qiushi; Li,Yuquan; Xu,Yuan.
An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychrophilic bacteria; Cold-adapted agarase; Purification; Characterization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300683

Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1-7 isolated from Bohai Bay in China for detergent formulation
Provedor de dados: 58
Autores: Wang,Haikuan; Zhong,Shaojiong; Ma,Huijing; Zhang,Jie; Qi,Wei.
A novel alkaline lipase-producing strain 1-7 identified as Acinetobacter calcoaceticus was isolated from soil samples collected from Bohai Bay, China, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. The lipase from strain 1-7 showed the maximum activity at pH 9.0 under 40ºC. One interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. It is also stable at a broad range of pHs from 4.0 to 10.0 for 24 h. Its catalytic activity was highly enhanced in the presence of Ca2+, Mg2+ and K+, but partially inhibited by Cu2+, Al3+, Fe3+ , Ba2+and Zn2+. The fact that it displays marked stability and activity in the presence of TritonX-100, Tween-20, Tween-80,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaline lipase; Acinetobacter calcoaceticus; Characterization; Detergent.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100016

Biosynthesis, purification and characterization of endoglucanase from a xylanase producing strain Aspergillus niger B03
Provedor de dados: 58
Autores: Dobrev,Georgi Todorov; Zhekova,Boriana Yordanova.
An extracellular endoglucanase was isolated from the culture liquid of xylanase producing strain Aspergillus niger B03. The enzyme was purified to a homogenous form, using consecutive ultrafiltration, anion exchange chromatography, and gel filtration. Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 28,800 Da determined by gel filtration. The optimal pH and temperature values for the enzyme action were 3.5 and 65ºC respectively. Endoglucanase was stable at 40ºC, pH 3.0 for 210 min. The substrate specificity of the enzyme was determined with carboxymethyl cellulose, filter paper, and different glycosides. Endoglucanase displayed maximum activity in the case...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endoglucanase; Purification; Characterization; Aspergillus niger; Endoxylanase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100008

Biodiversity and phylogenetic analysis of culturable bacteria indigenous to Khewra salt mine of Pakistan and their industrial importance
Provedor de dados: 58
Autores: Akhtar,Nasrin; Ghauri,Muhammad A.; Iqbal,Aamira; Anwar,Munir A.; Akhtar,Kalsoom.
Culturable bacterial biodiversity and industrial importance of the isolates indigenous to Khewra salt mine, Pakistan was assessed. PCR Amplification of 16S rDNA of isolates was carried out by using universal primers FD1 and rP1and products were sequenced commercially. These gene sequences were compared with other gene sequences in the GenBank databases to find the closely related sequences. The alignment of these sequences with sequences available from GenBank database was carried out to construct a phylogenetic tree for these bacteria. These genes were deposited to GenBank and accession numbers were obtained. Most of the isolates belonged to different species of genus Bacillus, sharing 92-99% 16S rDNA identity with the respective type strain. Other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; Characterization; Highly saline environments; PCR amplification of 16S rDNA; Industrially important enzymes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100029

Chitinase Production by Streptomyces sp. ANU 6277
Provedor de dados: 58
Autores: Narayana,Kolla J.P.; Vijayalakshmi,Muvva.
Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35ºC. Culture medium amended with 1% chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield.Chitinase was purified from crude enzyme extract by single step gel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitinase; Streptomyces sp. ANU 6277; Optimization; Characterization.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400002

Optimization and characterization of alkaline protease and carboxymethyl-cellulase produced by Bacillus pumillus grown on Ficus nitida wastes
Provedor de dados: 58
Autores: Gomaa,Eman Zakaria.
The potentiality of 23 bacterial isolates to produce alkaline protease and carboxymethyl-cellulase (CMCase) on Ficus nitida wastes was investigated. Bacillus pumillus ATCC7061 was selected as the most potent bacterial strain for the production of both enzymes. It was found that the optimum production of protease and CMCase were recorded at 30 °C, 5% Ficus nitida leaves and incubation period of 72 h. The best nitrogen sources for protease and CMCase production were yeast extract and casein, respectively. Also maximum protease and CMCase production were reported at pH 9 and pH 10, respectively. The enzymes possessed a good stability over a pH range of 8-10, expressed their maximum activities at pH10 and temperature range of 30-50 °C, expressed their maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaliphillic; Protease; Carboxymethylcellulase; Production; Characterization.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200033

Purification and Partial characterization of manganese peroxidase from Bacillus pumilus AND Paenibacillus sp.
Provedor de dados: 58
Autores: Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina.
The production of manganese peroxidase (MnP) from Bacillus pumilus and Paenibacillus sp. was studied under absence and presence of the inducers indulin AT, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. Indulin AT increased the activity of B. pumilus MnP up to 31.66 U/L after 8 h, but no improve was observed for Paenibacillus sp., which reached maximum activity (12.22 U/L) after 20 h. Both MnPs produced by these microorganisms were purified in phenyl sepharose resin and the proteins from crude extracts were eluted in two fractions. However, only the first fraction of each extract exhibited MnP activities. Tests in different pH and temperature values, from pH 5.0 to pH 10.0 and 30 ºC to 60 ºC, respectively, were carried...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Manganese peroxidase; Purification; Characterization.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400012

Collagenolytic enzymes produced by fungi: a systematic review
Provedor de dados: 58
Autores: Wanderley,Maria Carolina de Albuquerque; Duarte Neto,José Manoel Wanderley; Lima Filho,José Luiz de; Lima,Carolina de Albuquerque; Teixeira,José António Couto; Porto,Ana Lúcia Figueiredo.
Abstract Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenase) AND (fungi OR fungus OR fungal) AND (production OR synthesis OR synthesize) AND (characterization). Scientific criteria were adopted in this review to classify found articles by score (from 0 to 10). After exclusion criteria, 21 articles were selected. None obtained the maximum of 10 points defined by the methodology, which indicates a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagenase; Fungus; Characterization; Purification; Production.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100013

Characterization of a chitinase with antifungal activity from a native Serratia marcescens B4A
Provedor de dados: 58
Autores: Zarei,Mandana; Aminzadeh,Saeed; Zolgharnein,Hossein; Safahieh,Alireza; Daliri,Morteza; Noghabi,Kambiz Akbari; Ghoroghi,Ahmad; Motallebi,Abbasali.
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45ºC. Enzyme was stable in 55ºC for 20 min and at a pH range of 3-9 for 90 min at 25ºC. When the temperature was raised to 60ºC, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal; Characterization; Chitinase; Serratia marcescens B4A.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300022

Development and optimization of erythromycin-loaded lipid-based gel by Taguchi design: In vitro characterization and antimicrobial evaluation
Provedor de dados: 60
Autores: Dhillon,Pallavi; Mirza,Mohd. Aamir; Anwer,Md. Khalid; Alshetaili,Abdullah Saud; Alshahrani,Saad Maria; Iqbal,Zeenat.
The foremost aim of the current research was to prolong and sustain the release of erythromycin (ERY) by preparing a solid lipid nanoparticles (SLNs)-based gel formulation for the safe and effective treatment of acne. ERY-loaded SLNs were developed, and various process variables were optimized with respect to particle size, zeta potential, and entrapment efficiency using the Taguchi model. The average particle size, PDI, zeta potential, drug entrapment efficiency, and drug loading of optimized SLN (F4) were found to be 176.2±1.82 nm, 0.275±0.011, -34.0±0.84, 73.56%, and 69.74% respectively. The optimized SLN (F4) was successfully incorporated into the carbopol-based hydrogel. The in vitro release of ERY from the SLN gel and plain gel were compared and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythromycin; Solid lipid nanoparticles; Nanogel; Taguchi model; Characterization; Diffusion disc.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100583

Isolation and characterization of novel lectins from Canavalia ensiformis DC and Dioclea grandiflora Mart. ex Benth. seeds
Provedor de dados: 61
Autores: Melgarejo,Luz Marina; Vega,Nohora; Pérez,Gerardo.
Two lectins were isolated from Canavalia ensiformis and Dioclea grandiflora seeds. Gel filtration produced a fraction corresponding to Con A or D. grandiflora lectin while erythroagglutination assays revealed a distinct fraction presenting a lectin that agglutinates human red blood cells (RBCs) but not rabbit RBCs. Hydrophobic interaction chromatography showed that the latter fraction yielded a protein that readily agglutinates human erythrocytes; the lectin was also purified by affinity chromatography on Lac-Sepharose showing similar properties to that of the Phenyl-Sepharose-purified lectin. Despite minor differences (carbohydrate content or A1%1cm), the two lectins showed similar molecular properties in that they consisted of two non-covalently linked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diocleinae; Leguminoseae; Characterization; Lectins.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000300006

Analysis of Agromorphological Descriptors to Differentiate between Duke Cherry (Prunus x gondouinii (Poit. &amp;amp; Turpin)Rehd.) and Its Progenitors: Sweet Cherry (Prunus avium L.) and Sour Cherry (Prunus cerasus L.)
Provedor de dados: 117
Autores: Pérez,Rodrigo; Navarro,Florentino; Sánchez,María Ángeles; Ortíz,Jesús María; Morales,Remedios.
The rapid identification of the hybrids between sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.) is not easy. In order to resolve this problem, 18 Spanish sweet, sour and duke cherry cultivars were surveyed and characterized using 43 agromorphological descriptors evaluated in flowers, leaves, dormant 1-yr-old shoots, fruits, and trees during 2005 and 2006. Based on quantitative parameters, ANOVA and stepwise discriminant analysis (SDA) were carried out. For qualitative descriptors, statistical comparisons were done by means of the chi-square (&amp;#967;2) test. As result of the study, two quantitative (titratable acidity and number of lenticels) and six qualitative descriptors (shape of the central and lateral lobes in the internal bracts...
Tipo: Journal article
Palavras-chave: Rosaceae; Characterization; Quantitative parameters; Qualitative parameters; SDA analysis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000100004

Ecogeography of Lippia rotundifolia Cham. in Minas Gerais, Brazil
Provedor de dados: 65
Autores: Meira,Messulan Rodrigues; Martins,Ernane Ronie; Resende,Luciane Vilela.
ABSTRACT:  Lippia rotundifolia is a specie native to the Brazilian Cerrado, endemic to the Cadeia do Espinhaço mountain range. Due to the limited information about the species, the present study aimed to characterize the ecogeography, climate conditions and physical and chemical characteristics of the soil of Lippia rotundifolia in the state of Minas Gerais, Brazil. Thirteen sites were ecogeographically characterized: Parque Estadual Veredas do Peruaçu; Gigante; Rio do Peixe; AEP of Olhos D’água; Joaquim Felício; Parque Estadual do Rio Preto; São Gonçalo do Rio das Pedras; Rio Tigre; RPPN Brumas do Espinhaço; Lapinha; Poço Bonito; Abóboras; and Parque Estadual de Serra Nova. Environments belonged to Cerrado and Caatinga biomes, specifically in rocky and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic resources; Characterization; Ecogeography; Lippia rotundifolia.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800403