Consulta: subjectFacets:"Immunohistochemistry"
Registros recuperados: 124
Data/hora: 09/06/2024 09:21:08

Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin.
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members...
Tipo: Text
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf

Investigation of Various Events Occurring in the Brain Tissue After Calvarial Defects in Rats
Provedor de dados: 77
Autores: Tas,Mahmut; Gok,Ertugrul; Ekinci,Cenap; Deveci,Engin; Deveci,Senay.
Bone damage and accidents, traumas can alter people's normal life, and damage the soft tissues. In this study, we aimed to investigate in calvarial defects in rats depending on the severity of cerebral contusion injury occurring in the temporal region. The rats were randomly divided into two groups: group 1 (control group), critical size cranial model with no treatment (n= 10); group 2 (14-day synthetic graft group given 7th day DEXA), critical size cranial model treated with Dexamethasone (0.05 mg/kg intramuscular injection) +Synthetic graft (n= 10) One calvarium defect of 7 mm was made in the parietal bone of each animal under general anesthesia. Calvarial defect results in dilatation of blood vessels, hemorrhage and deterioration was observed in glial...
Tipo: Journal article
Palavras-chave: Calvarial defects; Rat; Cerebrum; Immunohistochemistry.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100005

Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice
Provedor de dados: 77
Autores: Rengifo,Aura Caterine; Umbarila,Vanessa Jazmin; Garzón,Mary Janeth; Torres-Fernández,Orlando.
Rabies is a lethal disease caused by a neurotropic virus that produces inconspicuous morphological changes hardly observable with conventional histopathology. The fatal outcome caused by rabies could be attributed to specific biochemical changes that severely impact neuronal function. The neuronal nuclear protein (NeuN) has become a widely used neuronal marker for the research and the histopathological diagnosis of nervous system diseases. To evaluate the distribution of the protein NeuN in the motor cortex of normal and rabies-infected mice adult ICR mice were inoculated with rabies virus either intramuscularly or intracerebrally. Rabies-infected mice were sacrificed at the terminal stage of the disease. Control mice were also euthanized at the same age....
Tipo: Journal article
Palavras-chave: Rabies; NeuN; Cerebral cortex; Mouse; Immunohistochemistry; Neurons.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400031

Expression of Mmp-20 in Dental Germs of Human Fetus
Provedor de dados: 77
Autores: Gutiérrez Cantú,Francisco Javier; Mariel Cárdenas,Jairo; Oliva Rodríguez,Ricardo; Romo Ramírez,Gabriel Fernando; Sánchez Méraz,Wulfrano; Mariel Murga,Humberto; Leal Tobias,Luis Armando.
During experiments in animal studies, it has been observed that enamelysin (MMP-20) is expressed during tooth development in the late secretory stage of amelogenesis but not in the mature enamel.The aim of this research was to determine the location of MMP-20 in human tooth germs in the different structures of the enamel organ.The detection of MMP-20 was performed by immunohistochemistry in 20 specimens obtained from human fetuses. Immunostaining of MMP-20 was observed from the presecretor stadium in stellate reticulum and intermediate stratum and in the basal portion of ameloblasts in the secretory stage in stellate reticulum, stratum intermedium, secretory ameloblasts, odontoblasts and dental papilla. The results of this research show the location of...
Tipo: Journal article
Palavras-chave: Enamelysin; Immunohistochemistry; Ameloblasts; Odontoblasts.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400022

Anti-inflammatory Effect of Silymarin on Ovarian Immunohistochemical Localization of TNF- Associated with Systemic Inflammation in Polycystic Ovarian Syndrome
Provedor de dados: 77
Autores: Kayedpoor,Parvin; Mohamadi,Shima; Karimzadeh-Bardei,Latifeh; Nabiuni,Mohammad.
Tumor necrosis factor alpha (TNF-a) and interleukin (IL)-6, are prominent mediators of inflammation and have been confirmed to be elevated in at least a subgroup of women with polycystic ovary syndrome (PCOS). In this study, the effects of Silymarin (SLM) on the expression TNF-a, IL-6, CRP and symptoms of PCOS were studied. In this research, PCOS was induced by injection of Estradiol Valerate. PCOS rats were divided into control and experimental groups received intraperitoneal injection SLM extract daily. After syndrome induction, ovaries were collected for histological and immunohistochemical evaluations. Serum IL-6 was detected by the ELISA kit. The results indicated the significant reduction in inflammatory markers and significant changes follicular...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; Inflammation; Silybum Marianum extract.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200054

Distribution and Morphology of Ghrelin-Immunopositive Cells in the Thymus of the African Ostrich
Provedor de dados: 77
Autores: Wang,Jia-xiang; Li,Peng; Zhang,Xiao-ting; Ye,Li-xun.
Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, has been detected in the thymus of multiple vertebrates. However, little is known about its distribution in the thymus of the African ostrich. In this study, we evaluated the distribution and morphological characteristics of ghrelin-producing cells in the thymus of the African ostrich. Our results revealed that the thymus consists of a capsule and a parenchyma, which comprises the cortex and medulla. Compared to the cortex, the medulla had a fewer number of thymocytes and a greater number of epithelial cells. Additionally, three thymic corpuscles were identified. Ghrelin-immunopositive (ghrelin-ip) cells were detected both in the cortex and medulla of the African ostrich thymus,...
Tipo: Journal article
Palavras-chave: African ostrich; Ghrelin; Thymus; Immunohistochemistry.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200026

Comparison of Proliferative Activity of Epithelial Cells between the Follicles of Impacted Teeth
Provedor de dados: 77
Autores: Kömerik,Nurgül; Tufan,Alper; Kapucuoglu,Nilgün.
The aim of this comparative observational study was to compare the proliferative activity of dental follicles surrounding impacted maxillary canines and mandibular third molars. Following extraction, forty follicles of the impacted mandibular third molars and 40 follicles of the impacted maxillary canines were removed. Epithelial cell proliferative activity of these samples was assessed using immunohistochemical labeling for Ki-67, minichromosome maintenance 2 (MCM-2) protein and epithelial growth factor receptor (EGFR). Intensity and extent of Ki-67, MCM-2 and EGFR expressions were evaluated by a scoring formula. The lining epithelium of the maxillary canine follicles had mean scores of 4.65±0.27 for Ki-67, 1.25±0.33 for MCM-2 and 7.30±0.23 for EGFR which...
Tipo: Journal article
Palavras-chave: Dental follicle; Ki-67; MCM-2; EGFR; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400028

p53 Immunoexpression in Oral Squamous Cell Carcinomas from Different Anatomical Sites: A Comparative Study
Provedor de dados: 77
Autores: Sá,Cláudia T.; Fonseca,Linaena M. S.; Cardoso,Sérgio V.; Aguiar,Maria Cássia F.; Carmo,Maria Auxiliadora V. do.
p53 alterations have been implicated in oral carcinogenesis process. Squamous cell carcinoma is the most common malignancy of the oral cavity, the tongue being the most frequent affected site. The aim of this study was to compare p53 immunoexpression in 43 samples of oral SCC from 5 different anatomical sites: tongue, floor of the mouth, alveolar ridge/gingiva, retromolar region and other regions of the oral cavity. Thirty-three lesions (76,8%) showed positivity for p53 (mean index of 48,37%). No statistically significant differences were found between p53 expression and the different oral sites analyzed. Similarly, no differences were found when comparing tongue lesions with the remaining sites. These results suggest that it might not exist any difference...
Tipo: Journal article
Palavras-chave: P53 protein; Immunohistochemistry; Oral squamous cell carcinoma (OSCC).
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300018

Histochemical Localization of Carbohydrates in Morphological Stages of Developing Human Minor Salivary Glands: A Comparative Study with Cytoskeletal Markers
Provedor de dados: 77
Autores: Rêgo,Moacyr J. B. M; Cavalacanti,Carmelita L. B; Beltrão,Eduardo I. C; Sobral,Ana Paula V.
Carbohydrates play a critical role in many cellular processes like disease, growth and development. In this work lectins, proteins that recognizes carbohydrate free or conjugated, were used as histochemical probes for carbohydrates localization in developing human minor salivary gland. Immunohistochemistry for traditional cytoskeleton markers (Cks 7, 8, 13, 14, 19, SMA and Vimentin) was performed and then compared whit lectin histochemistry for PNA, WGA, ConA and UEA-I, specifics for D-galactose, N-acetyl-glucosamine, glucose/mannose and L-fucose respectively. For this, specimens were obtained from tongues and lips of 15 human foetuses at 10-28 weeks of gestation. None of immune cytoskeleton markers were identified in the first stage of development...
Tipo: Journal article
Palavras-chave: Lectin histochemistry; Immunohistochemistry; Developing human minor salivary glands.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200051

Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice
Provedor de dados: 77
Autores: Hurtado,Andrea P; Rengifo,Aura Caterine; Torres-Fernández,Orlando.
The microtubule-associated protein MAP-2 is an integral part of the cytoskeleton and plays an important role in neural morphogenesis. This protein is an essential component of the dendritic cytoskeleton, especially in the adult brain, and its expression can be altered under experimental or pathological conditions. The purpose of this study was to evaluate the effect of infection with the rabies virus on MAP-2 immunoreactivity in the cerebral cortex of mice. The mice were inoculated with the rabies virus and the animals were sacrificed when the disease reached its advanced stage, together with uninfected animals of the same age. The brains were extracted after being previously perfusion-fixed with paraformaldehyde; coronal sections were obtained with a...
Tipo: Journal article
Palavras-chave: MAP-2; Cerebral cortex; Pyramidal neurons; Rabies virus; Immunohistochemistry.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200010

Unilateral Renal Haemangiopericytoma: Report of a Rare Tumour
Provedor de dados: 77
Autores: Ribeiro,J. G. A.; Klojda,C. A. B.; Machado,F. A.; Favorito,L. A.; Cavalcante,A. G. L.; Babinski,M. A.
The pericytes are modified smooth muscle cells (SMCs) that surrounding capillaries and to change the diameter of vascular lumen. In this article we report a case of renal haemangiopericytoma. A 78-year-old woman was admitted with a complaint of abdominal pain. Ultrasonography revealed a solid injury in the third portion of the left kidney. Abdominal CT scan revealed a tumoral mass. Due the high index of suspicion of malignant neoplasia the patient was submitted to left radical nefrectomy. The histopathological evaluation confirmed haemangiopericytoma by immunohistochemistry. The patient fared well and was alive 5 years after radical nephrectomy
Tipo: Journal article
Palavras-chave: Kidney; Tumour; Haemangiopericytoma; Pathology; Immunohistochemistry.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300007

Immunohistochemical Analysis of Proliferating Cell Nuclear Antigen (PCNA) in Dental Follicles of Impacted Third Molars
Provedor de dados: 77
Autores: Oliveira,David Moraes de; Silveira,Márcia Maria Ferreira da; Andrade,Emanuel Sávio de Souza; Sobral,Ana Paula Veras; Martins-Filho,Paulo Ricardo Saquete; Santos,Thiago de Santana; Oliveira,Patrícia Leimig Amorim de.
This study investigated the immunodetection of PCNA in epithelial components of dental follicles associated with impacted third molars without radiographical and morphological signs of pathosis. A total of 105 specimens of dental follicles associated with impacted third molars with incomplete rhizogenesis (between Nolla's stage 6 and 9) were surgically removed from 56 patients. Epithelial cell proliferating was determined by using immunohistochemical labeling. Statistical analysis was performed using the Fisher exact test. Of the 105 dental follicles collected, 6 were PCNA-positive ( 6%). The specimens with squamous metaplasia and epithelial hyperplasia had higher rates of positivity for PCNA, as well as those with proliferative remnants of odontogenic...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; Dental Sac; Third Molar.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200037

Expression of Neuronal and Non-Neuronal Markers in the Vomeronasal Organs of One-Humped Camel (Camelus dromedarius) and Egyptian Water Buffalo (Bubalus bubalis)
Provedor de dados: 77
Autores: Emam,Mahmoud Abdelghaffar; El-Shafey,Anwar A; Kassab,Ahmed A.
Fourteen vomeronasal organs (VNOs) of adult males one-humped camels (Camelus dromedarius) and Egyptian water buffaloes (Bubalus bubalis) (n=7/each) were examined immunohistochemically with neuronal markers; synaptophysin (SYP) and glial fibrillary acidic protein (GFAP) to clarify the distribution of the vomeronasal (VN) receptor cells and nerve fibers, in addition to elucidate the existence of non-neuronal elements via S-100 and endothelial nitric oxide synthase (eNOS). In both animals, the VNO was lined medially with VN sensory (olfactory) epithelium and non-sensory (respiratory) epithelium laterally. Immunohistochemically, both animals showed SYP immunolabeling only in the receptor cells of VN sensory epithelium while GFAP labeled the ensheathing cells...
Tipo: Journal article
Palavras-chave: Vomeronasal organ; Camel; Buffalo; Immunohistochemistry.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400006

Elastin Accumulation in Actinic Cheilitis with Different Degrees of Epithelial Dysplasia
Provedor de dados: 77
Autores: Araújo,Caliandra Pinto; Xavier,Flávia Caló de Aquino; Gurgel,Clarissa Araújo Silva; Ramos,Eduardo Antônio Gonçalves; Freitas,Valéria Souza; Schlaepfer-Sales,Caroline Brandi; Ramalho,Luciana Maria Pedreira; dos Santos,Jean Nunes.
The extracellular matrix (ECM) plays an important role in the regulation of biological events such as the development of cell migration, proliferation and differentiation. Chronic sun exposure causes changes present in the ECM of actinic cheilitis (AC), a premalignant lesion of the lower lip which helps to understand the carcinogenesis of the lip. This study aimed to investigate elastin, the main component of solar elastosis alternating current in an attempt to establish the relationship between this protein and ECM in epithelial dysplasia. Paraffin embedded tissue sections of the lesions of 35 cases of AC were analyzed by immunohistochemistry for elastin, and became the association with the degree of epithelial dysplasia and age. Highest scores of elastin...
Tipo: Journal article
Palavras-chave: Matrix extracellular; Elastin; Immunohistochemistry; Actinic cheilitis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200044

Death-Associated Protein Kinase is Underexpressed in High-Grade Oral Squamous Cell Carcinoma
Provedor de dados: 77
Autores: Meneses,Ismário Silva de; Souza,Rafael Reis de; Jeraldo,Verónica de Lourdes Sierpe; Cavalcante,Danielle Rodrigues Ribeiro; Reis,Francisco Prado; Albuquerque Júnior,Ricardo Luiz Cavalcanti de.
An immunohistochemical analysis of 40 cases of oral squamous cell carcinoma was performed to evaluate the relationship between the expression pattern of death-associated protein kinase (DAPk) positive cells with the histological malignancy grading of these lesions. According to our results, eleven cases (27.5%) were high-grade malignancy tumours and 29 (72.5%) were low-grade ones. We found that 92.86% of the low-grade tumours were positive to anti-DAP kinase antibody whereas only 7.14% of the high-grade tumours presented positivity, and this difference was statistically significant (p&lt;0.01). Sixteen (55.2%) of the low-grade carcinomas exhibited moderate immunoreactivity whereas ten cases (34.5%) showed weak staining and three cases (10.3%) were negative...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; DAP kinase; Apoptosis; Squamous cell carcinoma.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200044

Intraoral Mucoepidermoid Carcinoma of Salivary Glands: Lack of Association Among Clinicopathological Features and Immunoexpression of c-erbB-2 in 29 Cases
Provedor de dados: 77
Autores: Bernardes,Vanessa Fátima; Ramos-Jorge,Maria Letícia; Vieira Carmo,Maria Auxiliadora; Cardoso,Sérgio Vitorino; Alves Mesquita,Ricardo; Ferreira Aguiar,Maria Cássia.
The association among clinicopathological features and c-erbB-2 oncoprotein expression was evaluated in twenty-nine cases of intra-oral mucoepidermoid carcinoma (MEC). MEC was prevalent in the female gender (79.3%), tumors were more frequent in ages between 21 and 40 years (48.3%), and the palate was the most commonly affected site (72.4%). Microscopically, 27 cases (93.1%) were classified as low grade of malignancy. The c-erbB-2 expression was considered positive in 9 (31%) cases and no significant association (p&gt;0.05) was found among protein expression and gender ñor between patient age and site or histological grade of the lesión. c-erbB-2 expression in MEC may reflect intrisinc biologic properties of salivary gland neoplasms and may be linked to...
Tipo: Journal article
Palavras-chave: C-erbB-2; Her-2/neu; Immunohistochemistry; Mucoepidermoid carcinoma; Salivary gland neoplasms.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400036

Ultrastructural and Immunohistochemical Effects of Aqueous Leave Extract of Xylopia aethiopica on the Stomach in Streptozotocin-Induced Diabetic Rats
Provedor de dados: 77
Autores: Ofusori,D. A; Komolafe,O. A; Adewole,O. S; Arayombo,B. E; Margolis,D; Naicker,T.
Gastrointestinal pathology in diabetic patients has become a source of concern in recent times. The aim of this study was to investigate the ultrastructural and immunohistochemical effects of aqueous leaf extract of Xylopia aethiopica on the stomach in streptozotocin-induced diabetic rats. This study was conducted using thirty adult Wistar rats. The animals were divided into three groups (n= 10). Group A was the control animals (administered with equivalent volume of citrate buffer), group B was diabetic animals induced by a single intraperitoneal injection of streptozotocin dissolved in citrate buffer (65 mg/kg) and group C was diabetic animals treated with 200 mg/kg body weight of aqueous leave extract of X. aethiopica for twenty five days. At the...
Tipo: Journal article
Palavras-chave: Diabetes mellitus; Immunohistochemistry; Ultrastructure; Stomach; Xylopia aethiopic.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100006

Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods
Provedor de dados: 77
Autores: Millas,Ieda; Barros,Mirna Duarte; Curcio,Daniella Franco; Buck,Hudson Sousa; Liquidato,Bianca Maria.
Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to evaluate immunohistochemical staining intensity to detect the presence of ER in the nasal mucosa tissue: one using a digital system and the other through conventional direct microscopy observation. Sixty two stained samples were observed and analyzed under optic microscopy by three specialized professionals, who have graded intensity from: absent, mild, moderate and intense staining. Afterwards, an objective measurement was obtained by a relative optical density...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; Estrogen receptors; Methods; Microscopy.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400007

Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos
Provedor de dados: 77
Autores: Namm,Aimar; Arend,Andres; Aunapuu,Marina.
The role of bone morphogenetic proteins (BMP-s) in the development of the nervous system has been widely studied on avian and rodent embryos. Human embryos have rarely been available for detection of BMP expression. In this study 39 human embryos of Carnegie stages (CS) 10-20 were investigated. The embryos were fixed in paraformaldehyde, embedded in paraffin and sectioned serially in transverse direction. BMP-2 and BMP-4 protein expression in the developing neural tube and the caudal spinal cord was determined by immunohistochemistry. Our data show that BMP-s tend to be more expressed in the neural tube in earlier stages; in particular, BMP-4 staining was found to be higher at CS10 compared to CS20. More detailed analysis was performed on embryos of...
Tipo: Journal article
Palavras-chave: Neural tube; Human embryo; BMP-2; BMP-4; Immunohistochemistry.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200018

Immunohistochemical Study of Aquaporin 1 and 2 in the Reproductive Tract of the Bactrian camel (Camelus bactrianus)
Provedor de dados: 77
Autores: An,Fengli; Wang,Jianlin.
Spermatogenesis is associated with considerable fluid secretion or absorption in the male reproductive tract. Aquaporins (AQPs) are membrane protein channels that allow the rapid movement of water through epithelium. In the present study, immunohistochemistry was utilized to localize the expression of AQP 1, AQP2 in the testis and prostate of adult bactrian camel (Camelus bactrianus). Results show that AQP1 have intense reaction in rete testis, efferent ducts, vessels, seminiferous duct and in the prostate, AQP2 was found minor expression in the rete testis, vessels and prostate, which suggesting that AQP1 may have the main role in the absorption of the large amount of testicular fluid in male camel reproductive tract. Investigations of AQPs biology in...
Tipo: Journal article
Palavras-chave: Aquaporin1; Aquaporin2; Immunohistochemistry; Bactrian Camel.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400007

Inmunohistochemical Evaluation of Estrogen Receptors Alpha and Beta in Normal Inferior Turbinate Mucosa
Provedor de dados: 77
Autores: Millas,Ieda; Liquidato,Bianca Maria; Dolci,José Eduardo Lutaif; Macéa,José Rafael; Fregnani,José Humberto Tavares Guerreiro; Meceles,Lenira Rocha.
It has been postulated that the nasal mucosa, like other human tissues, is affected by a complex interactive network of neuropeptides, cytokines, allergic and inflammatory mediators and hormones such as estrogen, in which associations between symptoms (e.g. nasal stuffiness and coryza) and hormonal variations deriving from pregnancy, use of hormonal contraceptives and menstrual cycle phases are observed. The objective is evaluating the presence of specific estrogen receptors (types alpha and beta) in inferior turbinate mucosa in healthy subjects without nasal symptoms. Samples of nasal inferior turbinate were removed from patients undergoing aesthetic nasal surgery, and analyzed using hematoxylin-eosin staining, followed by immunohistochemical preparations...
Tipo: Journal article
Palavras-chave: Rhinitis; Estrogen receptors; Estrogens; Nasal mucosa; Hormones; Immunohistochemistry.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100020

ENVIRONMENTAL ENRICHMENT DURING EARLY POSTNATAL DEVELOPMENT DECREASES PARVALBUMIN EXPRESSION IN THE RAT SOMATOSENSORY CORTEX
Provedor de dados: 77
Autores: Inzunza,Oscar; Bravo,Hermes; Fernández,Víctor.
In the rat brain, parvalbumin (PV) expression starts on postnatal day 8 and comprises a heterogeneous population of nonpyramidal GABAergic neurons. In the present work, an immunohistochemical study was done on control and experimental rats submitted to enriched environmental conditions between postnatal days 3 to 18 or 3 to 24. Counts of PV+ neurons were made in the dorsomedial and in the ventrolateral regions of the somatosensory cortex. In control animals, PV+ neurons reached a peak on day 24 declining towards day 120. In these rats a peculiar distribution pattern was detected in which immunoreactive neurons are more numerous in the dorsomedial than ventrolateral regions as well in infragranular than supragranular layers and in posterior regions than...
Tipo: Journal article
Palavras-chave: Neuropeptide PV; Cerebral cortex; Polysensorial stimulation; Rodents; Immunohistochemistry.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300001

Localization of Cannabinoid Receptor 1 (CB1) in Submandibular and Sublingual Salivary Glands of Mice throughout Postnatal Development
Provedor de dados: 77
Autores: Hipkaeo,Wiphawi; Watanabe,Masahiko; Kondo,Hisatake.
In view of the fact that human marijuana users often show dry mouth symptom, the present study was attempted to examine the localization of CB1, which was originally identified in brain, in the submandibular and sublingual salivary glands of postnatal developing male mice by immunohistochemistry. In submandibular gland, CB1-immunoreactivity was positive in a majority of acinar cells in forms of granular appearance in their apical cytoplasm, while it was negative in the ducts at newborn stage. The immunoreactivity decreased in the acinar cells at P1W and no immunoreactivity was detected in the acinar cells at P3W and thereafter. The immunoreactivity was positive in ductal cells at P3W and it remained positive thereafter until P8W stage. The immunoreaction...
Tipo: Journal article
Palavras-chave: Cannabinoid receptor 1; Immunohistochemistry; Submandibular gland; Sublingual gland; Postnatal mice.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200044

Immunohistochemical Fiber Type Profile in the Human Vocal Muscle
Provedor de dados: 77
Autores: López,María José; Zárate,Michael; Cantín,Mario; Zavando,Daniela; Suazo Galdames,Iván.
The vocal muscle is a striated muscle with important functions in the emission of laryngeal sound and physiology of the voice. Therefore the knowledge of its constitution is the basis for the prevention and management of voice disorders. We used 10 samples from the middle third of vocal muscles obtained from autopsies of 6 male and 4 female subjects aged between 36 and 71 years. The samples were analyzed with BA-F8 monoclonal antibody to slow type I fibers, and antimyosin HC monoclonal antibody and antimyosin fast clone MY-32 antibody for types IIA, IIB, IIX, and neonatal fibers. We determined the distribution of the muscle fiber types and morphometric characteristics, evaluating the differences by sex and age group. The human vocal muscle presented a...
Tipo: Journal article
Palavras-chave: Voice; Vocal cords; Laryngeal muscles; Myosin Type I; Myosin Type II; Immunohistochemistry.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400014

Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)
Provedor de dados: 77
Autores: Rojas,Mariana; Saint-Pierre,Gustavo; Hartley,Ricardo; Vásquez,Bélgica; Conei,Daniel; del Sol,Mariano.
With the purpose of carrying out a diagnosis of the different pathologies that affect the salmon fry stage (Salmo salar) and analyze the regeneration phases of the organizational centers and subjacent tissue in case of an amputation, we realized a study that allowed identifying the temporary and spatial location of the Sonic Hedgehog (Shh) morphogen in hatched fry stage. Fifteen salmon fry (Salmo salar) were used. They were anesthetized with 5 % benzocaine (BZ-20®, Veterquímica), fixed in 10 % buffered formalin, and embedded in paraffin, Shh polyclonal antibody (Santa Cruz H-160, rabbit) was used diluted at 1/100. They were subsequently rinsed in PBS-1 % Triton and incubated with anti-rabbit conjugated polymer antibody and HRP for 10-15 min. The...
Tipo: Journal article
Palavras-chave: Sonic hedgehog; Salmon fry; Immunohistochemistry; Antibody antiShh.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200054

Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?
Provedor de dados: 77
Autores: Hipkaeo,Wiphawi; Suzuki,Ryoji; Abe,Hiroshi; Chaisiwamongkol,Kowit; Kondo,Hisatake.
After careful observation of immuno-electron microscopic findings of the renal glomerular podocyte slit so far published by various authors, it should be noted that no convincing evidence is present for localization of nephrin-immunoreactivity in the slit itself, but that nephrin-immunoreactivity is dominantly localized along the membranes of podocyte pedicles from their tip over a substantial extension. In addition, the notion that the slit diaphragm is composed of strands is compatible with the widely distributed view that the slit strands represent rod-shaped nephrin molecules. However, 3-D findings that the diaphragm is composed of not only strands but also membranous elements have recently demonstrated separately by embedment-free section electron...
Tipo: Journal article
Palavras-chave: Glomerulus; Slit; Nephrin; Localization; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400016

The Effects of Systemic Use of Nicotine on the Rat Nasal Mucosa: a Histopathologic and Immunohistochemical Study
Provedor de dados: 77
Autores: Yorgancilar,Ediz; Tunik,Selcuk; Deveci,Engin; Gun,Ramazan; Bakir,Salih; Kinis,Vefa; Ayaz,Ercan; Topcu,Ismail.
The objective of this study was to evaluate the histopathologic effects of systemic use of nicotine on the rat nasal mucosa. Twelve adult Sprague-Dawley rats weighing 180-220 g, were used as experimental animals. The rats were divided into Nicotine and control groups. The rats of Nicotine groups (n=6) were administered 2mg/kg Nicotine sulphate for 28 days. The rats of control group (n=6) were only administered 1,5 ml physiologic saline solution subcutaneously for 28 days. All animals were sacrified at the end of the study and nasal tissue samples were removed and prepared for histologic examination. The sections were stained with Hematoxylin and Eosin (H-E) and Periodic acid-Schiff (PAS) and Trichrome-Masson were observed under light microscope. E-cadherin...
Tipo: Journal article
Palavras-chave: Nicotine; Rat; Nasal mucosa; E-cadherin; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300010

Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat
Provedor de dados: 77
Autores: Núñez Chichet,M E; Genovese,P; Bielli,A.
The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO - is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with...
Tipo: Journal article
Palavras-chave: Vomeronasal organ; Chemoreception; Development; Histochemistry; Immunohistochemistry.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300018

Immunohistochemical Analysis of Human Homologue of Drosophila Patched (PTCH) in Dental Follicles of Impacted Third Molars
Provedor de dados: 77
Autores: Oliveira,David Moraes de; Silveira,Márcia Maria Ferreira da; Andrade,Emanuel Sávio de Souza; Sobral,Ana Paula Veras; Martins-Filho,Paulo Ricardo Saquete; Santos,Thiago de Santana; Oliveira,Patrícia Leimig Amorim de; Peixoto,Aline Carvalho; Santos,Jadson Alípio Santana de Souza; Piva,Marta Rabello.
This study investigated the immunodetection of PTCH in epithelial components of dental follicles associated with impacted third molars without radiographic signs of pathosis. One hundred and five specimens of dental follicles associated with impacted third molars with incomplete rhizogenesis (between Nolla's stage 6 and 9) were surgically removed from 56 patients. Epithelial cell proliferation was determined by using immunohistochemical labeling. Statistical analysis was performed using Fisher exact test and a level of significance of 5%. Of the 105 dental follicles collected, 3 were PTCH-positive. The specimens with squamous metaplasia and epithelial hyperplasia had higher rates of positivity for PTCH, as well as those with active remnants of odontogenic...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; Dental follicle; Odontogenic epithelial; PTCH.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100018

Immunohistochemical investigation of neuronal injury in cerebral cortex of cobra-envenomed rats
Provedor de dados: 80
Autores: Rahmy,T.R.; Hassona,I.A.
The immunohistochemical expression of neuron-specific enolase, NSE (a cytoplasmic glycolytic enzyme of the neurons), synaptophysin, SYN (a major membrane glycoprotein of synaptic vesicles), and Bcl-2 (anti-apoptotic protein) were determined in cerebral cortex of rats envenomed with neurotoxic venom from Egyptian cobra. Male rats were intramuscularly (IM) injected with a single injection of either physiological saline solution or ½ LD50 or LD50 of cobra venom and sacrificed 24, 48, or 72 hr after envenoming. Formalin-fixed paraffin sections were immunohistochemically studied by avidin-biotin-peroxidase complex method. Neuron histological structure and isolation of genomic DNA were also detected. The results showed a dose and time-dependent increase in NSE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunohistochemistry; Neuron specific enolase; Synaptophysin; Bcl-2; Cerebral cortex; Cobra snake venom.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100005

TNF-α and IL-6 immunohistochemistry in rat renal tissue experimentaly infected with Leptospira interrogans serovar Canicola
Provedor de dados: 80
Autores: Marinho,M; Monteiro,CMR; Peiró,JR; Machado,GF; Oliveira-Júnior,IS.
Leptospirosis is a public health problem worldwide and its etiology remains unclear. Its pathogenesis involves a complex interaction between host and infecting microorganism. The inflammatory reaction that controls the infection process also underscores many pathophysiological events occurring in leptospirosis. We investigated the presence of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in renal tissues by immunohistochemical and histopathological examination in animals experimentally inoculated with Leptospira serovar Canicola. All the tests were carried out 2, 7, 14, 21 or 28 days after inoculation. Although TNF-α and IL-6 had been detected in tissues throughout the observation period, these cytokines appeared more intensely during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Immunohistochemistry; IL-6; TNF-α; Mice.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300012

Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals
Provedor de dados: 19
Autores: Furuoka, Hidefumi; Yabuzoe, Atushi; Horiuchi, Motohiro; Tagawa, Yuichi; Yokoyama, Takashi; Yamakawa, Yoshio; Shinagawa, Morikazu; Sata, Tetsutaro.
For immunohistochemistry of the prion diseases, several pretreatment methods to enhance the immunoreactivity of human and animal abnormal proteinase-resistant prion protein (PrPSc) on the tissue sections have been employed. The method of 121 degrees C hydrated autoclaving pretreatment or the combination method of 121 degrees C hydrated autoclaving with a certain chemical reagent ( formic acid or proteinase K, etc) are now widely used. We found that an improved hydrated autoclaving method at 135 degrees C, more effectively enhanced PrPSc immunoreactivity for the antibodies recognizing the linear epitope. In addition, this method was more effective for the long-term fixation samples as compared with other previous methods. However, this modified method could...
Palavras-chave: Prion protein; Immunohistochemistry; Antigen retrieval; Autoclaving; Monoclonal antibody; CREUTZFELDT-JAKOB-DISEASE; MONOCLONAL-ANTIBODIES; IMMUNOCYTOCHEMISTRY; IMMUNOREACTIVITY; PROTOCOLS; FIXATION; DEPOSITS; PLAQUES; BRAIN; PRP.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/712

Detection of CD4+ and CD8+ lymphocytes in the intestine of broiler chicks treated with Lactobacillus spp. and challenged with Salmonella enterica serovar Enteritidis
Provedor de dados: 89
Autores: Noujaim,JC; Andreatti Filho,RL; Lima,ET de; Okamoto,AS.
The expression of immune response as a leukocytic infiltrate by CD4+ and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks fed Lactobacillus spp or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis (SE) was studied using immunohistochemistry. Three hundred and twenty day-of-hatch broiler chicks were divided into four groups of 80 birds each and orally received L. reuteri, L. salivarius, L. acidophilus, or CM. Each group was subdivided into four subgroups of 20 birds each, classified as follows: a subgroup did not receive any oral treatment (negative control), subgroup treated with L. spp or CM, subgroup treated with L. spp or CM and challenged with SE, and subgroup only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Immunohistochemistry; Lymphocytes CD4+ and CD8+; Probiotic; Salmonella.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300008

Pathological, immunohistochemical, and molecular findings in commercial laying hens and in backyard chickens naturally infected with the infectious laryngotracheitis virus
Provedor de dados: 89
Autores: Preis,IS; Fiúza,ATL; Silva,CC; Braga,JFV; Couto,RM; Martins,NR da S; Ecco,R.
Seventy-eight chickens from a very high poultry density (approximately eight million) region and twelve backyard chickens from neighboring areas were analyzed by histopathology and additional techniques for the presence of the infectious laryngotracheitis virus. The virus distribution was determined in different tissues using immunohistochemistry (IHC) and polymerase chain reaction (PCR). The disease was histopathologically diagnosed in 41.0% (32/78) of the commercial layers. Lesions were mainly characterized by syncytial cells with eosinophilic intranuclear inclusion body formed from the hyperplastic epithelium of the upper respiratory tract, primary and secondary bronchi, and conjunctiva. IHC showed 70% (21/30) positive signal in the larynx/trachea and,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian infectious laryngotracheitis; Conventional PCR; Histopathology; Immunohistochemistry; Laying hen.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400004

การประเมินการเจริญเติบโตและการพัฒนาของคอร์ปัสลูเทียมแพะ โดยการวิเคราะห์โปรตีน ดีเอ็นเอ และ Proliferating cell nuclear antigen (PCNA)
Provedor de dados: 106
Autores: Jiratti Thammasiri; Chainarong Navanukraw; Srinuan Kananit; Paweena Pongdontri; Theera Rittirod.
The objective of the present study was to evaluate growth and development of corpus luteum (CL) using protein, DNA analysis and PCNA labeling index throughout the estrous cycle in goats. Non pregnant goats (n=20), 12 months of age were assigned for estrus synchronization. Goats CL were obtained from days 3, 8, 13 and 18 of estrous cycle using bilateral ovariectomy and blood samples were taken for plasma progesterone (P4) concentration. The CL was weighed, and samples of each were fixed in Carnoy’s solution. Additional samples of each CL were frozen and stored at -70 °C until analyzed for tissue DNA and protein. Luteal fresh weight and DNA contents increased (P&lt;0.01) from day 3 to day 8, were similar between days 8 and 13, and then decreased (P&lt;0.01)...
Tipo: PhysicalObject
Palavras-chave: Goat; Corpus luteum; Oestrus cycle; DNA analysis; Protein; PCNA labeling index; Progesterone hormone; Protein analysis; DNA analysis; Growth indicator; Immunohistochemistry; แพะ; คอร์ปัสลูเทียม; การเป็นสัด; การวิเคราะห์ปริมาณโปรตีน; การวิเคราะห์ดีเอ็นเอ; ดีเอ็นเอ; โปรตีน; เซลล์จำเพาะ; ฮอร์โมนโปรเจสเทอโรน; ดัชนีชี้วัดการเจริญเติบโต.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5810

Administering ascorbic acid to rats undergoing ageing processes: effects on myosin-V immunoreactive myenteric neurons
Provedor de dados: 42
Autores: Mello,Sônia A.; Marese,Angélica C.M.; Brancalhão,Rose M.C.; Zanoni,Jacqueline N.; Natali,Maria Raquel M.
During the ageing process the enteric nervous system undergoes morphofunctional changes, such as enteric neurodegeneration. Neuronal death can be attributed to increase radicals free, and ascorbic acid (AA), known antioxidant, could minimize damage cause by oxidative stress. The objective of this study is to analyse the behaviour of morphoquantative myenteric neurons in the duodenum of adult Wistar rats with aged 90 (C90), 345 (E345) and 428 (E428) days, as well as animals of the same age who received ascorbic acid supplementation for 120 days (EA345 and EA428). Whole-mount preparations of muscle layer from the duodenum of the animals were immunostained by the method myosin V. 80 microscopic fields were quantified (14.8 mm2/animal) and measured 100...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Antioxidant; Enteric nervous system; Immunohistochemistry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100337

Ontogenic development of kidney, thymus and spleen and phenotypic expression of CD3 and CD4 receptors on the lymphocytes of cobia (Rachycentroncanadum )
Provedor de dados: 42
Autores: KLOSTERHOFF,MARTA C.; PEREIRA JÚNIOR,JOABER; RODRIGUES,RICARDO V.; GUSMÃO,EMELINE P.; SAMPAIO,LUÍS A.; TESSER,MARCELO B.; ROMANO,LUIS A.
ABSTRACT In the present study was evaluated the ontogenic of immunocompetent organs of cobia up to 53 days after hatching (dah) through histology and immunohistochemistry techniques. The kidney was the first lymphohematopoietic organ to appear, at 1 dah, followed by the spleen at 5 dah and the thymus at 7 dah. The first CD3 receptors on the lymphocytes were observed in 27% of the thymic tissue at 7 dah and in 99% at 53 dah. The phenotypic expression of CD3 receptors was registered in 10% of the kidney at 8 dah and in 32% at 53 dah. CD4 receptors were observed in 5% and 63% of the thymic area at 7 and 53 dah, respectively. In the kidney, T4 lymphocytes were first observed at 13 dah in 9% of the organ and in 28% at 53 dah, defining the functional development...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cobia; Immune system; Ontogeny; Immunohistochemistry; Lymphoid organs.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502111

Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis
Provedor de dados: 42
Autores: NUNES-TAVARES,NILSON; CUNHA-E-SILVA,NARCISA LEAL; HASSÓN-VOLOCH,AÍDA.
Acetylcholine is the neurotransmitter responsible for the transmission of impulses from cholinergic neurons to cells of innervated tissues. Its biosynthesis is catalyzed by the enzyme Choline acetyltransferase that is considered to be a phenotypically specific marker for cholinergic system. It is well known that the regulation of Choline acetyltransferase activity under physiological and pathological conditions is important for development and neuronal activities of cholinergic functions. We observed the distribution of Choline acetyltransferase in sections from the normal and denervated main electric organ sections of Electrophorus electricus (L.) by immunofluorescence using a anti-Choline acetyltransferase antibody. The animals were submitted to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Choline acetyltransferase; Electrocyte; Denervation; Immunohistochemistry.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300007

Antifibrotic effects of total or partial application of amniotic membrane in hepatic fibrosis
Provedor de dados: 42
Autores: MAMEDE,KARINA M.; SANT’ANNA,LUCIANA B.
Abstract: Liver fibrosis is the final common pathway of chronic liver diseases, having cirrhosis as a possible progression, which has liver transplantation as the only effective treatment. Human amniotic membrane represents a potential strategy as a therapy for liver fibrosis, due to its anti-inflammatory, anti-fibrotic and immunomodulatory properties. The aim of this study was to evaluate amniotic membrane effects as a treatment for hepatic fibrosis induced in rats by bile duct ligation (BDL), verifying alterations between two different forms of amniotic membrane application, around all the lobes of the liver and around only one lobe of the liver. Two weeks after inducing fibrosis, an amniotic membrane fragment was applied to the surface of the liver,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amniotic membrane; Histopathology; Immunohistochemistry; Liver fibrosis; Myofibroblasts.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500702

Spindle cell sarcoma in Bothrops leucurus
Provedor de dados: 45
Autores: Marcello Jr.,H. B.; Maciel,R.; Ribeiro,C.A.; Caliari,M.V.; Cassali,G.D.
O presente relato descreve os achados anatomopatológicos de uma neoplasia maligna em jararaca (Bothrops leucurus), mantida em cativeiro durante sete anos. O animal apresentava massa nodular subcutânea localizada no lado direito do terço anterior. O exame histológico revelou tratar-se de neoplasia mesenquimal maligna, permitindo o diagnóstico de sarcoma fusocelular.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; Sarcoma; Neoplasm; Immunohistochemistry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300018

A new methodology for the improvement of diagnostic immunohistochemistry in canine veterinary pathology: automated system using human monoclonal and polyclonal antibodies
Provedor de dados: 45
Autores: Cassali,G.D.; Silva,P.; Rêma,A.; Gärtner,F.; Gobbi,H.; Tafuri,W.L.; Schmitt,F.C.
The authors describe their experience with an automated immunohistochemical system applied to canine tissue samples. Twenty human cellular markers specific monoclonal and polyclonal antibodies and two different antigen retrieval methods were used in normal and neoplastic breast tissue, as well as skin samples obtained from female dogs of pure and mixed breeds. The antibodies tested were the most frequently used in human and veterinary medicine studies, employed with diagnostic purposes in breast pathology, as well as in cancer research. Most of them may be used to study other normal and abnormal tissues and included cytokeratins, progesterone receptor, c-erbB2, p53, MIB-1, PCNA, EMA, vimentin, desmin, alpha-actin, S-100, pan-cadherin, and E-cadherin. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Immunohistochemistry; Automated system; Mammary gland.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000300010

DNA flow cytometry of canine mammary tumors: comparative aspects with human breast tumors
Provedor de dados: 45
Autores: Cassali,G.D.; Salvador,A.; Freitas,C.; Dutra,A.P.; Schmitt,F.C.
Flow cytometric analysis of DNA content was performed on 28 samples of canine mammary tumors. Nine of them were benign and 19 were malignant. All benign tumors and 11 malignant tumors (57.9%) were diploid (P&lt;0.05). Form the aneuploid tumors, five (26.3%) were hyperdiploid, one (5.3%) hypodiploid, one (5.3%) near triploid and one (5.3%) multiploid. The analysis of the expression of the markers PR and CD31 revealed a significant difference between diploid and aneuploid tumors (P&lt;0.05). The immunoreactivity of PR was higher in diploid tumors, while the immunoreactivity of CD31 was stronger in aneuploid tumors. No difference between the markers MIB-1, c-erbB2, p53 and Cyclin D1 was observed (P&gt;0.05). Using the flow cytometry analysis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Breast cancer; Neoplasm; DNA flow cytometry; Immunohistochemistry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500011

Electroretinography and immunohistochemistry of retina in rabbits treated with sildenafil citrate
Provedor de dados: 45
Autores: Amaral,A.V.C.; Silva,G.A.; Costa,A.P.A.; Oliveira,K.S.; Jordão,P.; Ribeiro,A.P.; Renzo,R.; Laus,J.L.
Sildenafil citrate is a type-5 phosphodiesterase inhibitor (PDE-5), able to inhibit type-6 phosphodiesterase (PDE-6) as well, providing clinical benefits and paraeffects, some of them potentially related to the retina. The effects of the sildenafil on the retrobulbar and retinal circulation were studied in 27 adult male rabbits of the White New Zealand breed. The electric activity of the retina was evaluated before and at the end of the treatments, and immunohistochemistry studies were conducted. An amplitude increase of the b wave was found in the mixed response of cones and rods after 7 days of treatment with sildenafil citrate. However, in the other evaluations and periods, the values did not differ from the basal ones. Through immunohistochemistry, no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabbit; Sildenafil citrate; Electroretinography; Immunohistochemistry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601589

Comparative study of histopathology and immunohistochemistry of indefinite round cell cutaneous tumors and characterization of canine lymphoma
Provedor de dados: 45
Autores: Machado,L.H.A.; Palumbo,M.I.P.; Zahn,F.S.; Amorim,R.L.; Farias,M.R.; Werner,J.; Torres Neto,R.; Rodrigues,J.C.; Oliveira,F.C.
With the purpose of shedding light on some doubts in veterinary oncology, the present article intends to compare the results of histopathological and immunohistochemical examinations of unspecific round cell neoplasia, to realize immunophenotyping of canine lymphoma cases, to establish the T or B origin of neoplastic cells, and to determine the degree of proliferation and apoptosis of lymphomas by immunohistochemistry. Of 11 animals presenting immunohistochemical diagnosis of lymphoma, five had been diagnosed as Lymphoma by HE staining of histopathological slides and six had been classified as unspecific round cell neoplasia. All cases submitted to immunohistochemical examination were T-cell lymphomas. There was a positive correlation between cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Histopathology; Immunohistochemistry; Round cell neoplasia; Lymphoma.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100032

Bovine respiratory syncytial virus: immunohistochemichal detection in mouse and bovine tissues using a Mab against human respiratory syncytial virus
Provedor de dados: 45
Autores: Almeida,R.S.; Spilki,F.R.; Roehe,P.M.; Verinaud,L.M.C.; Arns,C.W.
An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine respiratory syncytial virus; Immunohistochemistry; Calf; Mice; Experimental infection; Monoclonal antibody.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600001

Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil
Provedor de dados: 45
Autores: Leal,J.S.; Correa,G.L.F.; Boos,G.S.; Bianchi,M.V.; Boabaid,F.M.; Lopes,R.F.F.; Driemeier,D.
Scrapie is a fatal and progressive transmissible spongiform encephalopathy (TSE) of natural occurrence in sheep and goats. The suspicion of scrapie may be based on clinical signs; however, the detection of pathological features of the prionic protein (PrP) in target tissues is necessary to diagnose the disease. The presence of an abnormal protein form (PrPSc) in lymphoreticular and nervous tissues is an important characteristic in diagnosis. This paper reports a case of scrapie in a flock of 55 Suffolk crossbred sheep, 19 Santa Inês sheep and 21 goats in the Mato Grosso state, midwestern Brazil. The animals were euthanized after the confirmation of a scrapie case with clinical signs in a Suffolk sheep in the same farm. Samples of brainstem at the level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scrapie; Prion; Diseases of small ruminants; Immunohistochemistry; Lymphoid tissues.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601625

Analysis of EGFR and HER-2 expressions in ductal carcinomas in situ in canine mammary glands
Provedor de dados: 45
Autores: Silva,I.L.D.; Dias,A.P.M.; Bertagnolli,A.C.; Cassali,G.D.; Ferreira,E.
Biomolecular evidence has shown that ductal carcinoma in situ(DCIS) may develop into invasive carcinoma of the canine mammary gland, and mutations in proto-oncogenes HER2 and EGFR; two members of the family of epidermal growth factor receptors, may be involved in this process. The purpose of this study was the characterization of the immunohistochemical expression of the EGFR and HER2 proteins in the process of neoplastic transformation, supposedly present in ductal carcinomas in situin canine mammary glands. Fifteen cases of DCIS were evaluated, with a higher expression of HER2 and EGFR being observed in low-grade carcinomas when compared with high-grade neoplasms, and with a high positive statistical correlation in the latter. Results suggest that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Neoplasm; Immunohistochemistry; EGFR; CerbB2.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300763

Insertion of the LINE-1 element in the C-MYC gene and immunoreactivity of C-MYC, p53, p21 and p27 proteins in different morphological patterns of the canine TVT
Provedor de dados: 45
Autores: Lima,C.R.O.; Faleiro,M.B.R.; Rabelo,R.E.; Vulcani,V.A.S.; Rubini,M.R.; Torres,F.A.G.; Moura,V.M.B.D.
ABSTRACT The canine transmissible venereal tumor (TVT) affects the external genitalia of dogs by the natural transplant of viable tumor cells. Thus, this research aimed to diagnose and characterize TVT morphological patterns, identify the insertion of the LINE-1 element in C-MYC gene, by means of the polymerase chain reaction (PCR), and evaluate the immunohistochemical expression of C-MYC, p53, p21 and p27 proteins. The relationship between C-MYC and p53 proteins and their interference on the expression of p21 and p27 were also studied. For that, 20 samples of naturally occurring TVT were used, subjected to cytopathological, histopathological and immunohistochemical analysis, and to molecular diagnosis of neoplasia. The increased tissue expression and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Cell; Cytology; Immunohistochemistry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300658

Reactivity of p53 protein in canine transmissible venereal tumor
Provedor de dados: 45
Autores: Moro,J.V.; Tinucci-Costa,M.; Silveira,A.C.T.; Gerardi,D.G.; Alessi,A.C.
The expression of p53 protein was evaluated in canine transmissible venereal tumor (CTVT), as following: natural occurrence (n=8); resistant to chemotherapy (n=4); and allogeneic transplanted in progression (n=8), stable (n=8), and regression (n=8)stages. The collected specimens were submitted to GM1 immunohistochemical reaction. Results showed a mean percentage of immunomarked cells around 18.6% in CTVT of natural occurrence, 23.8% in CTVT resistant to chemotherapy, 22.9% in allogeneic transplanted CTVT in both progression and stable stages, and 35.8% in transplanted CTVT in regression stage. The results suggest that there is a functional abnormality in p53 gene and its products in the studied tumors; although, it is not possible to correlate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunohistochemistry; P53; Oncology; Transmissible venereal tumor.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200011

Bovine viral diarrhea diagnostic: immunohistochemistry standardization for routine
Provedor de dados: 45
Autores: Andrade,G.I.; Serra,C.V.; Barbosa-Stancioli,E.F.; Serakides,R.; Lobato,Z.I.P.
The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best results were seen using monoclonal antibodies and a commercial kit consisting of labelled streptavidin biotin (LSAB) reagents and the diaminobenzidine (DAB) substrate-chromogen reagent. The immunohistochemistry demonstrated to be an useful method for routine diagnosis for the controll and detection of BVDV infection.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Diarréia bovina a vírus; Imunoistoquímica; Bovine viral diarrhea; Virus; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500018

Intestinal intussusception secondary to enteritis caused by Pythium insidiosum in a bitch: case report
Provedor de dados: 45
Autores: Firmino,M.O.; Frade,M.T.S.; Alves,R.C.; Maia,L.Â.; Olinda,R.G.; Ximenes,R.G.; Souza,A.P.; Dantas,A.F.M.
ABSTRACT We report a case of intestinal intussusception secondary to enteritis caused by Pythium insidiosum infection in a 1-year-old mixed breed bitch that died after a 30-day history of bloody diarrhea, anorexia, weight loss, listlessness, dehydration, and pale mucous membranes. Necropsy revealed two areas of intussusception, one jejunum-jejunal and one ileum-cecum-colic. The first intussusception showed slight congestion of the intestinal loop, which was resolved by manual traction, while the second intussusception exhibited thickening, irregular yellow spots, and transmural congestion. Histologically, the jejunum-jejunal segment had ischemic lesions secondary and ileum-cecum-colic intussusception there was also necrotizing, pyogranulomatous enteritis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pythiosis; Pyogranulomatous enteritis; Immunohistochemistry; Intestinal intussusception; Pythium insidiosum.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300623

Histopathological and immunohistochemical study of the gastrointestinal tract from a dog naturally infected with Leishmania (Leishmania) chagasi: a case report
Provedor de dados: 45
Autores: Silva,F.L.; Tafuri,W.L.; Oliveira,M.R.; Tafuri,Wg. L.
Samples of stomach, duodenum, jejunun, ileum, cecum and colon were collected for Giemsa-smears ("imprints") from one asymptomatic mongrel dog, naturally infected with Leishmania (L) chagasi. Other fragments were obtained and fixed in formalin (10% and buffered) for histopathological and immunohistochemical studies. The immunohistochemistry was carried out by a streptavidin-peroxidase technique and it allowed to detect amastigote forms of Leishmania chagasi in the different paraffin gut sections. The principal lesion observed was a discrete to moderate chronic inflammatory reaction in the mucosa and submucosa in all fragments of the gastrointestinal tract (GIT). A chronic cellular exsudate was observed in all GIT tissues and it was composed by mononuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Visceral leishmaniasis; Histopathology; Immunohistochemistry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000400002

Metastatic well differentiated squamous cell carcinoma in the prepuce of a dog: a report of clinicopathological, immunophenotypic and therapeutic approach
Provedor de dados: 45
Autores: Auler,P.A.; Gamba,C.O.; Horta,R.S.; Lavalle,G.E.; Cassali,G.D.
This report describes a case of a well differentiated squamous cell carcinoma (SCC) of the foreskin of a dog, with metastasis in the regional lymph node. A six-year-old male intact Pit Bull dog presented a preputial ulcerated lesion with an evolution time of one year and enlarged left inguinal lymph node. Surgical resection of the preputial lesion and inguinal lymph nodes was made. The diagnosis of a well differentiated SCC was made following histopathological analysis and immunohistochemistry technique was used to confirm lymph node metastasis and to determine the histological features of the tumor. Here we demonstrated that immunohistochemistry can be an important complementary diagnostic tool to assess the prognostic features of SCC and to determine the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metastasis; Tumor; Skin; Immunohistochemistry; Chemotherapy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501317

Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil
Provedor de dados: 45
Autores: Estima-Silva,P.; Riet-Correa,F.; Coelho,A.C.B.; Echenique,J.V.Z.; Marcolongo-Pereira,C.; Lima,M.; Diel,D.G.; Schild,A.L.
RESUMO Foi realizado um estudo retrospectivo dos casos suspeitos de aborto por herpesvírus equino observados na região sul do Rio Grande do Sul entre 1978 e 2016. Foram revisados os protocolos de necropsia do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas resgatando-se os dados epidemiológicos, lesões macroscópicas e histológicas de cada caso. Foram observados dois surtos da enfermidade com prevalência entre 5,7% e 50% nos diferentes estabelecimentos, e dois casos individuais. Em todos os casos foram enviados fragmentos de órgãos fetais formolizados. Histologicamente, em todos os casos foram observados focos de necrose no fígado, pulmão e baço e presença de corpúsculos de inclusão acidofílico em...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Abortion; Equine herpesvirus-1; Immunohistochemistry; PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401421

Parotid gland adenocarcinoma in a cow: case report
Provedor de dados: 45
Autores: Queiroz,P.J.B.; Queiroz,T.D.; Magalhães,P.L.; Borges,N.C.; Martins,D.B.; Moura,V.M.B.D.; Silva,L.A.F.
ABSTRACT A 9-year-old Girolando dairy cow, weighing 400kg, with a history of increased volume in the right parotid region, which extended to the submandibular region, was assisted. Fine needle aspiration cytology was performed, and the cytological findings were consistent with malignant neoplasm of epithelial origin (carcinoma). Because of the unfavorable prognosis, the animal was euthanized and submitted to an anatomopathological examination. Samples of the increased parotid and affected lymph nodes were collected for histopathological evaluation. The microscopic changes were accentuated features of anaplasia, moderate cell proliferation, atypical mitotic figures, and necrosis. Stroma ranged from delicate to scirrhous, and the tumor boundaries were not...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Cattle; Cytology; Immunohistochemistry; Malignant neoplasms; Salivary glands.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300758

Clinical and pathological aspects of multicentric hemangiosarcoma in a Pinscher dog
Provedor de dados: 45
Autores: Martins,B.D.C.; Torres,B.B.J.; Rodriguez,A.A.M.; Gamba,C.O.; Cassali,G.D.; Lavalle,G.E.; Martins,G.D.C.; Melo,E.G.
A 3-year-old female Pinscher dog was presented with a history of lameness and pain in hind limbs for one week which acutely progressed to non-ambulatory paraparesis. The dog had been apathetic and anorexic during the six days prior to presentation. The neurological findings were compatible with upper motor neuron signs to the left hind limb due to a spinal cord compressive mass on the twelfth thoracic vertebral body. On the other hand, signs of lower motor neurons to the right hind limb were due to a mass with a pathological fracture in the right proximal femur, compressing the sciatic nerve. A histopathological analysis of all organs revealedthe proliferation of endothelial cells showingin filtrative growth and organization into vascular structures with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Neuropathy; Hemangiosarcoma; CD-31; Immunohistochemistry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000200003

Epitheliotropic cutaneous T-cell lymphoma associated with melanoma in a dog: case report
Provedor de dados: 45
Autores: Palumbo,M.I.P.; Rossetto,V.J.V.; Torres Neto,R.; Brandão,C.V.S.; Amorim,R.L.; Fabris,V.E.; Machado,L.H.A.
Several types of tumors affect dogs' skin. Simultaneously occurring neoplasms with different histological patterns might be rarely present in the same animal. This paper describes the occurrence of epitheliotropic cutaneous T-cell lymphoma and melanoma in a dog. The animal had nodular lesions in the abdominal region and serpiginous plaques on the dorsal region of the trunk. Cytology evidenced malignant fusiform cells from the abdominal lesions as well as few round cells from the dorsal. The histopathological examination of the abdominal lesions showed dermis with polygonal to spindle-shaped neoplastic cells. The lesion of the dorsal region evidenced neoplastic round cells with generally distinct cell borders and a moderate amount of eosinophilic cytoplasm....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oncology; Immunohistochemistry; Malignant skin neoplasms.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300664

Tubulopapillary carcinoma of the mammary gland in a maned wolf (Chrysocyon brachyurus): histopathological and immunophenotypical analysis
Provedor de dados: 45
Autores: Gamba,C.O.; Damasceno,K.A.; Ferreira,E.; Malta,M.C.C.; Cassali,G.D.
A maned female wolf (Chrysocyon brachyurus) showed nodules in the inguinal and left abdominal cranial mammary glands. The mammary gland was surgically excised, and microscopic analysis revealed epithelial cell proliferation in a tubular and papillary pattern; delicate fibrovascular stalks presenting numerous layers of moderately pleomorfic epithelial cells were observed. This histologic appearance was compatible with a diagnosis of mammary tubulopapillary carcinoma. The immunohistochemical profile revealed nuclear positivity for estrogen (70%) and progesterone (at least 90% of the neoplastic cells). The myoepithelium-associated with neoplastic cells lacked integrity, as evidenced by failed smooth muscle alpha actin reactivity in microinvasive areas. A low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chrysocyon brachyurus; Female maned wolf; Immunohistochemistry; Mammary gland; Tubulopapillary carcinoma.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600014

Pythiosis with atypical location in the soft palate in a horse in Southern Brazil
Provedor de dados: 45
Autores: Rossato,C.K.; Fiss,L.; Sperotto,V.R.; Cardona,R.O.C.; Silva,R.B.
RESUMO A pitiose é uma enfermidade piogranulomatosa causada pelo oomiceto aquático Pythium insidiosum, que acomete várias espécies domésticas, assim como a humana. O presente relato refere-se à descrição de um caso de pitiose em um equino, fêmea, da raça Crioula, criado na região Sul do Brasil com história clínica de emagrecimento progressivo, disfagia e dificuldade respiratória. Macroscopicamente, o palato mole estava aumentado de tamanho e deslocado caudalmente, resultando em obstrução física da nasofaringe. Ao corte, essa massa era branca-amarelada, fibrosa e com áreas de ulceração. Na análise histopatológica, havia inflamação piogranulomatosa, com focos necróticos associados a imagens negativas tubuliformes de hifas fúngicas. A coloração de Grocott...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Equine; Atypical pythiosis; Pythium insidiosum; Immunohistochemistry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200641

Correlative microscopy of Purkinje cells
Provedor de dados: 47
Autores: Castejón,Orlando J.
The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebellar cells; Synaptic contacts; Electron microscopy; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100001

Histomorphological and quantitative immunohistochemical changes in the rat pancreas during aging
Provedor de dados: 47
Autores: Riccillo,F.L.; Bracamonte,M.I.; Cónsole,G.M.; Gómez Dumm,C.L.A.
Although the endocrine pancreas is the purpose of several deep investigations, morphological data referred to the effect of aging on the gland are not homogeneous. The purpose of the current work was to analyze the changes occurring in the pancreas of aged rats, with especial reference to the islet cell populations. Six young (Y), old (O) and senescent (S) male Sprague-Dawley rats were used. The pancreas tails were processed for light microscopy and studied by means of routine stains as well as by immunohistochemical identification of insulin-, glucagon-, somatostatin-, and pancreatic polypeptide- secreting cells (Dako Envision System, DAB as chromogen). A progressive pancreatic histoarchitecture distortion was found among the aged animals. Even when the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Pancreas; Islets; Immunohistochemistry; Morphometry.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200001

Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver
Provedor de dados: 47
Autores: Martín,Carlos A.; Surur,José Miguel; García,Marcela N.; Corrons,Félix; Badrán,Amado F.
Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3 ± 3.4 SE) located at 16:00/54 Time of Day / Hours Post- Hepatectomy (TD/HPH), which were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver regeneration; Sinusoid littoral cell; Bromodeoxyuridine; AgNOR; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100005

Distribution of delta sleep-inducing peptide in the newborn and infant human hypothalamus: an immunohistochemical study
Provedor de dados: 48
Autores: NAJIMI,MOHAMED; BENNIS,MOHAMED; MOYSES,EMMANUEL; CHIGR,FATIHA.
The distribution of delta sleep-inducing peptide immunoreactive cell bodies, fibers, and terminal-like structures was investigated in the normal human hypothalamus during the first postnatal year, using immunohistofluorescence and peroxidase anti-peroxidase techniques. Immunolabeled perikarya were relatively few and were mostly scattered through the anterior (preoptic) and mediobasal regions (infundibular nucleus) of the hypothalamus. DSIP-immunoreactive fibers and terminal-like fibers were observed throughout the entire rostro-caudal extent of the hypothalamus. They exhibit high densities in the preoptic region, the organum vasculosum of lamina terminalis, infundibular nucleus and median eminence. Moderate to low densities of DSIP-immunoreactive fibers...
Tipo: Journal article
Palavras-chave: DSIP; Immunohistochemistry; Development; Post-mortem; Human brain.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100013

Expression of non-neuronal cholinergic system in maxilla of rat in vivo
Provedor de dados: 48
Autores: Guo,Jie; Wang,Lue; Xu,Haihua; Che,Xiaoxia.
BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits &amp;#945;1, &amp;#945;2, &amp;#945;3, &amp;#945;5,...
Tipo: Journal article
Palavras-chave: Non-neuronal cholinergic system; Maxilla; Real-Time PCR; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100066

Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
Provedor de dados: 48
Autores: Shen,Cong-Cong; Kang,Yu-Huan; Yu,Lin; Cui,Dan-Dan; He,Yi; Yang,Jin-Liang; Gou,Lan-Tu.
BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; TEX101; Seminoma; Testis; Western blotting.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100075

Protein Expression and Codon 72 Polymorphism of TP53 Gene in Triple Negative Breast Cancer
Provedor de dados: 52
Autores: Lopes,Leandra Fiori; Guembarovski,Roberta Losi; Guembarovski,Alda Losi; Kishima,Marina Okuyama; Campos,Clodoaldo Zago; Derossi,Daniela Rudgeri; Ariza,Carolina Batista; Ozawa,Patricia Midori Murobushi; Oliveira,Carlos Eduardo Coral de; Banin-Hirata,Bruna Karina; Vitiello,Glauco Akelinghton Freire; Borelli,Sueli Donizete; Watanabe,Maria Angelica Ehara.
A subgroup of tumor that has received attention is triple-negative breast cancer (TNBC), which presents phenotype of negative estrogen receptor, negative progesterone receptor and has no overexpression of HER2. TP53 acts as a tumor suppressor limiting the proliferation of damaged cells. A polymorphic site (rs1042522) of TP53 encodes either an arginine or a proline amino acid, but its biological significance remains unclear. This study aimed to investigate this variant and its expression in search for a possible involvement in TNBC susceptibility and clinical outcome. Genetic polymorphism was evaluated in 50 patients and 115 controls by PCR based methodology and immunohistochemistry was done with monoclonal antibody. Case-control study showed no positive or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; TNBC; TP53; Genetic polymorphism; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600895

Expression of Ghrelin and GHSR-1a in Long Term Diabetic Rat's Kidney
Provedor de dados: 52
Autores: Yildirim,Aysegul Burcin; Karabulut,Derya; Dundar,Munis; Ulusoy,Hasan Basri; Sonmez,Mehmet Fatih.
The aim of this work was to study the relative ghrelin and growth hormone secretagogue receptor (GHS-R)1a gene expression in the kidney of long-term diabetic rats. Forty male Wistar albino rats were divided into four groups: C- control group, DI- one month diabetic rats group, DII- two months diabetic rats group, and DIII- three months diabetic rats group. Diabetes was induced by streptozotocin STZ (40mg/kg i.p). The rats were decapitated under ketamine anesthesia and their kidney tissues were removed. Tissue GHS-R mRNA levels, ghrelin expression, and histopathological damage scores were compared. Dilatation in the distal tubules, epithelial desquamation into the lumen of the tubules and transparent tubules showing glycogen vacuolation were observed in all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Ghrelin; GHSR-1a; Kidney; Immunohistochemistry; RT-PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100300

Toxoplasmosis in emperor tamarin (Saguinus imperator): case report
Provedor de dados: 62
Autores: EPIPHANIO,Sabrina; CATÃO-DIAS,José Luiz; GUIMARÃES,Marcelo Alcindo de Barros Vaz.
A case of toxoplasmosis in an adult male emperor tamarin (Saguinus imperator) is reported. The primate was found dead and no clinical sign was noticed before death. Pathological findings included moderate to severe interstitial pneumonia, multifocal necrotizing hepatitis and multifocal to coalescing necrotizing lymphadenitis. Immunohistochemistry assays (strepto-avidin-biotin-peroxidase) performed on paraffin embedded tissues (lung, lymph nodes, liver, spleen, heart, intestine and adipose tissue) were strongly positive for Toxoplasma gondii.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Primates; Saguinus imperator; Immunohistochemistry.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000200003

Syzygium cumini and the regeneration of insulin positive cells from the pancreatic duct
Provedor de dados: 62
Autores: Schossler,Deila Rosély C.; Mazzanti,Cinthia Melazzo; Luz,Sônia Cristina Almeida da; Filappi,Andreane; Prestes,Danívia; Silveira,Aron Ferreira da; Cecim,Marcelo.
Syzygium cumini is a plant that has been used in popular medicine for the treatment of insulin dependent diabetes mellitus (DMID). This study verified the effect of Syzygium cumini upon the regeneration of insulin producing cells in the pancreatic duct wall. The animals were divided into four groups, control (C), treated control (TC), diabetic control (DC) and treated diabetic (TD). An aqueous extract from Syzygium cumini bark was given by gavage in a daily dose of 1g/kg of body weight. After a thirty day period the animals were euthanized and the pancreas taken to immunohistochemical analysis. In this study, it was observed the positive staining for insulin on cells of the pancreatic duct and connective tissue in the pancreas of TD and TC animals. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Syzygium cumini; Alloxan; Diabetes; Immunohistochemistry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000400003

Granulosa cell tumour with metastasis in a cat
Provedor de dados: 62
Autores: GIACÓIA,Márcia Regina; MAIORKA,Paulo César; OLIVEIRA,Clair Motos; SINHORINI,Idércio Luiz; DAGLI,Maria Lúcia Zaidan.
The clinical, gross and microscopical features of a granulosa cell tumour in the ovary of a 2-year-old cat are described. This is a rare tumour in cats, moreover because of the animal’s age. It was diagnosed clinically by ultrasound examination. The tumour, a cystic mass in the region of the left ovary, metastasized to the epiplon and lungs. Tumour cells expressed vimentin and were arranged in a diffuse sarcomatous pattern, supported by a fine fibrovascular stroma. The presence of hair loss and repeated oestrus as clinical signs are indicative of orthoendocrine paraneoplastic syndrome, due to excessive oestrogen stimulation. This tumour should be considered in the differential diagnosis of behavioural disturbances in female cats.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Granulosa cell tumour; Ovary; Cats; Immunohistochemistry; Paraneoplastic syndromes.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000500005

p53 as a prognostic factor in adrenocortical tumors of adults and children
Provedor de dados: 56
Autores: Sredni,S.T.; Zerbini,M.C.N.; Latorre,M.R.; Alves,V.A.F.
Mutations of the tumor suppressor gene p53 have been considered to be important determinants in several kinds of human cancer. Accumulation of p53 protein has been reported to correlate with more aggressive clinical behavior in some neoplasms. The role of p53 expression in adrenal cortical tumors (ACT) has not been elucidated but some studies have suggested its correlation with malignant behavior. Our objective was to determine if there is a correlation between the expression of immunoreactive p53 and the biological behavior of ACT. Fifty-seven ACT (21 from children and 36 from adults) were evaluated for p53 expression by immunohistochemistry in formalin-fixed paraffin-embedded tissue and analyzed in terms of outcome. The p53 parameter was utilized...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Adrenal cortical tumors; Histology; P53; Immunohistochemistry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100004

Detection of human parvovirus B19 in a patient with hepatitis
Provedor de dados: 56
Autores: Pinho,J.R.R.; Alves,V.A.F.; Vieira,A.F.; Moralez,M.O.S.; Fonseca,L.E.P.; Guz,B.; Wakamatsu,A.; Cançado,E.L.R.; Carrilho,F.J.; da Silva,L.C.; Bernardini,A.P.; Durigon,E.L.
Parvovirus B19 has been associated by some investigators with cases of severe hepatitis. The aim of the present study was to determine the presence of active parvovirus B19 infection among 129 Brazilian patients with non-A-E hepatitis. The patients were assayed for antibodies against parvovirus B19, IgM class, by ELISA. In IgM-positive cases, parvovirus B19 DNA was assayed by PCR in serum and liver tissue and parvovirus VP1 antigen in liver tissue was assayed by immunohistochemistry. Antibodies against parvovirus B19, IgM class, were detected in 3 (2.3%) of 129 patients with non-A-E hepatitis. Previous surgery and blood transfusions were reported by these 3 patients. One patient was a 56-year-old female with severe hepatitis, with antimitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parvovirus B19; Hepatitis non-A-E; Autoimmune hepatitis; Polymerase chain reaction; Immunohistochemistry.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900005

Expression of p53 protein in pituitary adenomas
Provedor de dados: 56
Autores: Oliveira,M.C.; Marroni,C.P.; Pizarro,C.B.; Pereira-Lima,J.F.; Barbosa-Coutinho,L.M.; Ferreira,N.P.
Inactivating mutations of TP53, a tumor suppressor gene, are associated with abnormal cell proliferation. Although p53 expression is common in many human malignancies, p53 protein has seldom been evaluated in pituitary tumors. When detected, the percentage of p53-positive cells is low, and, in general, it is exclusive for invasive lesions. The aim of the present study was to use immunohistochemistry to determine the presence of p53 protein in pituitary adenomas from tumor samples of 163 surgeries performed in 148 patients (40% male, 60% female). In 35% of the cases the adenoma was nonfunctional, while in the others it was associated with PRL, GH and/or ACTH endocrine hypersecretion syndrome. Macroadenomas were observed in 83.2% of the cases with available...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pituitary; Pituitary adenoma; Invasive adenoma; P53; Immunohistochemistry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500008

Monoamines in the pedal plexus of the land snail Megalobulimus oblongus (Gastropoda, Pulmonata)
Provedor de dados: 56
Autores: Faccioni-Heuser,M.C.; Zancan,D.M.; Achaval,M.
In molluscs, the number of peripheral neurons far exceeds those found in the central nervous system. Although previous studies on the morphology of the peripheral nervous system exist, details of its organization remain unknown. Moreover, the foot of the terrestrial species has been studied less than that of the aquatic species. As this knowledge is essential for our experimental model, the pulmonate gastropod Megalobulimus oblongus, the aim of the present study was to investigate monoamines in the pedal plexus of this snail using two procedures: glyoxylic acid histofluorescence to identify monoaminergic structures, and the unlabeled antibody peroxidase anti-peroxidase method using antiserum to detect the serotonergic component of the plexus. Adult land...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pedal plexus; Catecholamines; Serotonin; Glyoxylic acid; Immunohistochemistry; Gastropod.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700014

Distribution of biglycan and decorin in rat dental tissue
Provedor de dados: 56
Autores: Tenório,D.M.H.; Santos,M.F.; Zorn,T.M.T.
Biglycan and decorin are small leucine-rich proteoglycans that play several biological and structural roles in different tissues and organs. Several reports have indicated that biglycan participates in odontoblast and ameloblast differentiation and in the calcification process. In the present study we show that the expression of biglycan changes from within the ameloblasts and odontoblasts to the extracellular space according to the stage of animal development. In predentin and in the pulp space, however, biglycan was continually expressed throughout the period of investigation. In contrast, decorin was absent in odontoblasts and in ameloblasts and was exclusively expressed in predentin throughout the period of observation. In young rats, however, decorin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decorin; Biglycan; Odontoblasts; Predentin; Immunohistochemistry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800012

MMP-1/PAR-1 signal transduction axis and its prognostic impact in esophageal squamous cell carcinoma
Provedor de dados: 56
Autores: Peng,Hong-hua; Zhang,Xi; Cao,Pei-guo.
The matrix metalloprotease-1 (MMP-1)/protease-activated receptor-1 (PAR-1) signal transduction axis plays an important role in tumorigenesis. To explore the expression and prognostic value of MMP-1 and PAR-1 in esophageal squamous cell carcinoma (ESCC), we evaluated the expression of two proteins in resected specimens from 85 patients with ESCC by immunohistochemistry. Sixty-two (72.9%) and 58 (68.2%) tumors were MMP-1- and PAR-1-positive, respectively, while no significant staining was observed in normal esophageal squamous epithelium. MMP-1 and PAR-1 overexpression was significantly associated with tumor node metastasis (TNM) stage and regional lymph node involvement. Patients with MMP-1- and PAR-1-positive tumors, respectively, had poorer disease-free...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix metalloprotease-1; Protease-activated receptor-1; Esophageal squamous cell carcinoma; Prognosis; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100014

Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
Provedor de dados: 56
Autores: Hermel,E.E.S.; Severino,G.S.; Cecconello,A.L.; Pereira,F.M.; Sanvitto,G.L.; Lucion,A.B.
Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotin-peroxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Stress; Neonatal stimulation; Dopamine; Immunohistochemistry; Hypothalamus; Tyrosine hydroxylase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900013

Prognostic significance of snail expression in hilar cholangiocarcinoma
Provedor de dados: 56
Autores: Kong,Dalu; Liang,Jun; Li,Rong; Liu,Shihai; Wang,Jigang; Zhang,Kejun; Chen,Dong.
Many patients with hilar cholangiocarcinoma (HC) have a poor prognosis. Snail, a transcription factor and E-cadherin repressor, is a novel prognostic factor in many cancers. The aim of this study was to evaluate the relationship between snail and E-cadherin protein expression and the prognostic significance of snail expression in HC. We examined the protein expression of snail and E-cadherin in HC tissues from 47 patients (22 males and 25 females, mean age 61.2 years) using immunohistochemistry and RT-PCR. Proliferation rate was also evaluated in the same cases by the MIB1 index. High, low and negative snail protein expression was recorded in 18 (38%), 17 (36%), and 12 (26%) cases, respectively, and 40.4% (19/47) cases showed reduced E-cadherin protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hilar cholangiocarcinoma; Prognostic marker; Snail; E-cadherin; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700008

Tissue hyaluronan expression, as reflected in the sputum of lung cancer patients, is an indicator of malignancy
Provedor de dados: 56
Autores: Rangel,M.P.; de Sá,V.K.; Martins,V.; Martins,J.R.M.; Parra,E.R.; Mendes,A.; Andrade,P.C.; Reis,R.M.; Longatto-Filho,A.; Oliveira,C.Z.; Takagaki,T.; Carraro,D.M.; Nader,H.B.; Capelozzi,V.L.
Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronic acid; Sputum; Lung cancer; Immunohistochemistry; ELISA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600557

Lymphatic fluctuation in the parenchymal remodeling stage of acute interstitial pneumonia, organizing pneumonia, nonspecific interstitial pneumonia and idiopathic pulmonary fibrosis
Provedor de dados: 56
Autores: Parra,E.R.; Araujo,C.A.L.; Lombardi,J.G.; Ab’Saber,A.M.; Carvalho,C.R.R.; Kairalla,R.A.; Capelozzi,V.L.
Because the superficial lymphatics in the lungs are distributed in the subpleural, interlobular and peribroncovascular interstitium, lymphatic impairment may occur in the lungs of patients with idiopathic interstitial pneumonias (IIPs) and increase their severity. We investigated the distribution of lymphatics in different remodeling stages of IIPs by immunohistochemistry using the D2-40 antibody. Pulmonary tissue was obtained from 69 patients with acute interstitial pneumonia/diffuse alveolar damage (AIP/DAD, N = 24), cryptogenic organizing pneumonia/organizing pneumonia (COP/OP, N = 6), nonspecific interstitial pneumonia (NSIP/NSIP, N = 20), and idiopathic pulmonary fibrosis/usual interstitial pneumonia (IPF/UIP, N = 19). D2-40+ lymphatic in the lesions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphangiogenesis; Lymphatic vessel; Idiopathic interstitial pneumonias; Lung remodeling; Immunohistochemistry; Morphometry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500013

Cyclin D1 expression in prostate carcinoma
Provedor de dados: 56
Autores: Pereira,R.A.; Ravinal,R.C.; Costa,R.S.; Lima,M.S.; Tucci,S.; Muglia,V.F.; Dos Reis,R.B.; Silva,G.E.B.
The purpose of this study was to investigate the relationship between cyclin D1 expression and clinicopathological parameters in patients with prostate carcinoma. We assessed cyclin D1 expression by conventional immunohistochemistry in 85 patients who underwent radical prostatectomy for prostate carcinoma and 10 normal prostate tissue samples retrieved from autopsies. We measured nuclear immunostaining in the entire tumor area and based the results on the percentage of positive tumor cells. The preoperative prostate-specific antigen (PSA) level was 8.68±5.16 ng/mL (mean±SD). Cyclin D1 staining was positive (cyclin D1 expression in &gt;5% of tumor cells) in 64 cases (75.4%) and negative (cyclin D1 expression in ≤5% of tumor cells) in 21 cases (including 15...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Cyclin D1; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600515

p16INK4 expression in precursor lesions of squamous cell cervical cancer related to the presence of HPV-DNA
Provedor de dados: 56
Autores: Godoy,A.E.G.; Mandelli,J.; Oliveira,F.H.; Calegari,S.; Moura,L.B.; Serafini,E.P.
The purpose of the present study was to identify the expression of p16INK4 in cervical cancer precursor lesions by immunohistochemistry and to correlate it with lesion grade and presence of human papillomavirus (HPV) infection. Cervical specimens from 144 women seen consecutively at the gynecology outpatient clinic of our institution from December 2003 to May 2005 were analyzed by cytopathology, histopathology, polymerase chain reaction for HPV-DNA, and p16INK4 immunostaining. Histologically normal biopsies, HPV-DNA negative by polymerase chain reaction, were used as control. HPV-DNA prevalence, including the control group, was 68.1% and the prevalence of p16INK4 expression was 55.0%. The percentage of cells stained by p16INK4 ranged from 10 to 100%, both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P16INK4; Cervical cancer; Human papillomavirus; Immunohistochemistry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700006

Immunoexpression of cyclooxygenase-1 and -2 in ulcerative colitis
Provedor de dados: 56
Autores: Paiotti,A.P.R.; Artigiani Neto,R.; Forones,N.M.; Oshima,C.T.F.; Miszputen,S.J.; Franco,M.
Ulcerative colitis (UC) is a disease of the colon and rectum characterized by a nonspecific chronic inflammation mediated by the concerted response of cellular and humoral events. Prostaglandins are synthesized by cyclooxygenase (COX)-1 and -2 and exhibit both pro- and anti-inflammatory activity. To evaluate COX-1 and COX-2 immunoexpression in 42 cases of UC and to correlate it with clinicopathological parameters, COX-1 and COX-2 expression was investigated by the immunohistochemistry method. Only patients with all pertinent clinical and evolutive data as well as with adequate biopsy material were included in the study. Fifteen samples of colorectal adenocarcinoma and 14 of large bowel with no histological changes were used for positive and negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ulcerative colitis; Cyclooxygenases; Prostaglandins; Immunohistochemistry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700004

Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
Provedor de dados: 56
Autores: Sá,V.K. de; Rocha,T.P.; Moreira,AL.; Soares,F.A.; Takagaki,T.; Carvalho,L.; Nicholson,A.G.; Capelozzi,V.L.
We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, adenomatous hyperplasia, severe dysplasia, squamous cell carcinoma and adenocarcinoma. Matched lung parenchyma tissue samples included 25 squamous cell carcinomas and 31 adenocarcinomas. Immunohistochemistry was performed to analyze for the distribution of hyaluronidase (Hyal)-1 and −3, and hyaluronan synthases (HAS)-1, −2, and −3. Hyal-1 showed significantly higher expression in basal cell hyperplasia than in moderate dysplasia (P=0.01), atypical adenomatous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronidases and hyaluronan synthases; Lung cancer; Pre-neoplastic lung/bronchial lesions; Immunohistochemistry; Morphometry; Prognosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101039

Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats
Provedor de dados: 56
Autores: Aguiar,C.E.; Cadore,L.P.; Padoin,M.J.; Barbosa-Coutinho,L.M.; Lucion,A.B.
Immunohistochemistry was used to evaluate the effects of neonatal handling and aversive stimulation during the first 10 days of life on the number of corticotrophs in the anterior lobe of the pituitary of 11-day-old male Wistar rats. Since adult rats handled during infancy respond with reduced corticosterone secretion in response to stressors and with less behavior inhibition in novel environments, we assumed that neonatal stimulation could affect pituitary morphology during this critical period of cell differentiation. Three groups of animals were studied: intact (no manipulation, N = 5), handled (N = 5) and stimulated (submitted to 3 different aversive stimuli, N = 5). The percentage of ACTH-immunoreactive cells in the anterior lobe of the pituitary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACTH; Immunohistochemistry; Pituitary; Neonatal handling; Stress.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200013

Expression of neuronal nitric oxide synthase in the hippocampal formation in affective disorders
Provedor de dados: 56
Autores: Oliveira,R.M.W.; Guimarães,F.S.; Deakin,J.F.W.
Hippocampal output is increased in affective disorders and is mediated by increased glutamatergic input via N-methyl-D-aspartate (NMDA) receptor and moderated by antidepressant treatment. Activation of NMDA receptors by glutamate evokes the release of nitric oxide (NO) by the activation of neuronal nitric oxide synthase (nNOS). The human hippocampus contains a high density of NMDA receptors and nNOS-expressing neurons suggesting the existence of an NMDA-NO transduction pathway which can be involved in the pathogenesis of affective disorders. We tested the hypothesis that nNOS expression is increased in the human hippocampus from affectively ill patients. Immunocytochemistry was used to demonstrate nNOS-expressing neurons in sections obtained from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Affective disorders; Major depression; Neuronal nitric oxide synthase; Immunohistochemistry; Hippocampus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400012

Comparison of blood neoangiogenesis and lymphatic vascularization in colorectal adenomas from patients with and without concomitant colorectal cancer
Provedor de dados: 56
Autores: Moreira,L.R.; Schenka,A.A.; Latuf Filho,P.; Lima,C.S.P.; Trevisan,M.A.S.; Vassallo,J.
Blood and lymphatic vessel proliferation is essential for tumor growth and progression. Most colorectal carcinomas develop from adenomas (adenoma-carcinoma sequence) in a process due to accumulation of molecular genetic alterations. About 5% of adenomatous polyps are expected to become malignant, but data on the differential angiogenic patterns of these lesions in patients with and without concomitant cancer are missing. The aim of the present study is to compare the angiogenic and lymphatic patterns of adenomatous polyps from patients with and without sporadic cancer. Thirty adenomatous polyps (15 from patients with another principal malignant lesion, and 15 from patients without cancer) were submitted to immunohistochemical staining for CD105 (marker for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiogenesis; Colorectal adenoma; Colorectal cancer; Immunohistochemistry; CD105; D2-40.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700002

Changes in expression of BDNF and its receptors TrkB and p75NTR in the hippocampus of a dog model of chronic alcoholism and abstinence
Provedor de dados: 56
Autores: Xu,R.; Duan,S.R.; Zhao,J.W.; Wang,C.Y.
Chronic ethanol consumption can produce learning and memory deficits. Brain-derived neurotrophic factor (BDNF) and its receptors affect the pathogenesis of alcoholism. In this study, we examined the expression of BDNF, tropomyosin receptor kinase B (TrkB) and p75 neurotrophin receptor (p75NTR) in the hippocampus of a dog model of chronic alcoholism and abstinence. Twenty domestic dogs (9-10 months old, 15-20 kg; 10 males and 10 females) were obtained from Harbin Medical University. A stable alcoholism model was established through ad libitum feeding, and anti-alcohol drug treatment (Zhong Yao Jie Jiu Ling, the main ingredient was the stems of watermelon; developed in our laboratory), at low- and high-doses, was carried out. The Zhong Yao Jie Jiu Ling was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic alcoholism; Brain-derived neurotrophic factor; Tropomyosin receptor kinase B; P75 Neurotrophin receptor; Immunohistochemistry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800703

Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis
Provedor de dados: 56
Autores: Mendonça,D.M.F.; Chimelli,L.; Martinez,A.M.B.
Amyotrophic lateral sclerosis (ALS), a neurodegenerative disease of unknown etiology, affects motor neurons leading to atrophy of skeletal muscles, paralysis and death. There is evidence for the accumulation of neurofilaments (NF) in motor neurons of the spinal cord in ALS cases. NF are major structural elements of the neuronal cytoskeleton. They play an important role in cell architecture and differentiation and in the determination and maintenance of fiber caliber. They are composed of three different polypeptides: light (NF-L), medium (NF-M) and heavy (NF-H) subunits. In the present study, we performed a morphological and quantitative immunohistochemical analysis to evaluate the accumulation of NF and the presence of each subunit in control and ALS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyotrophic lateral sclerosis; Spheroids; Neurofilament proteins; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600015

Increased immunohistochemical expression of YKL-40 in the spleen of patients with portal hypertension
Provedor de dados: 56
Autores: Wang,Dong; Lu,Jian-Guo; Wang,Qing; Du,Xi-Lin; Dong,Rui; Wang,Peng; Zhao,Lei; Jiang,Xue; Yuan,Li-Juan.
YKL-40 has been identified as a growth factor in connective tissue cells and also a migration factor in vascular smooth muscle cells. To a large extent, the increase of serum YKL-40 is attributed to liver fibrosis and asthma. However, the relationship of the expression and clinical/prognostic significance of YKL-40 to the splenomegaly of patients with portal hypertension is unclear. In the present study, the expression of YKL-40 was studied by immunohistochemistry in 48 splenomegaly tissue samples from patients with portal hypertension and in 14 normal spleen specimens. All specimens were quickly stored at -80°C after resection. Primary antibodies YKL-40 (1:150 dilution, rabbit polyclonal IgG) and MMP-9 (1:200 dilution, rabbit monoclonal IgG) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YKL-40; MMP-9; Splenomegaly; Fibrosis; Portal hypertension; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300013

Differential patterns of myosin Va expression during the ontogenesis of the rat hippocampus
Provedor de dados: 56
Autores: Brinn,L.S.; Pereira Leite,J.; Larson,R.E.; Martins,A.R.
Myosin Va is an actin-based, processive molecular motor protein highly enriched in the nervous tissue of vertebrates. It has been associated with processes of cellular motility, which include organelle transport and neurite outgrowth. The in vivo expression of myosin Va protein in the developing nervous system of mammals has not yet been reported. We describe here the immunolocalization of myosin Va in the developing rat hippocampus. Coronal sections of the embryonic and postnatal rat hippocampus were probed with an affinity-purified, polyclonal anti-myosin Va antibody. Myosin Va was localized in the cytoplasm of granule cells in the dentate gyrus and of pyramidal cells in Ammon's horn formation. Myosin Va expression changed during development, being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myosin Va; Growth and development; Central nervous system; Hippocampus; Rat; Immunohistochemistry.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900012

Vertical growth phase and positive sentinel node in thin melanoma
Provedor de dados: 56
Autores: Oliveira Filho,R.S.; Ferreira,L.M.; Biasi,L.J.; Enokihara,M.M.S.S.; Paiva,G.R.; Wagner,J.
Sentinel node (SN) status is the most important prognostic factor for localized melanoma. Usually, patients with Breslow thickness of less than 1.0 mm are not included in SN protocols. However, the literature presents a rate ranging from 3 to 7% of nodal recurrence in thin melanoma. Ulceration, regression and high mitotic rate have been considered to be indications for an SN biopsy. The metastatic potential of the vertical growth phase is uncertain. To correlate pathological features in thin melanoma with SN metastasis, we reviewed 358 patients submitted to SN biopsy. Seventy-seven patients with lesions of 1 mm or smaller were included in the study group. Histological evaluation of the primary tumor included thickness, Clark level, mitotic rate,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thin melanoma; Sentinel node; Lymphoscintigraphy; Immunohistochemistry; Micrometastasis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300009

Immunohistochemical detection of virus through its nuclear cytopathic effect in idiopathic interstitial pneumonia other than acute exacerbation
Provedor de dados: 56
Autores: Santos,G.C. dos; Parra,E.R.; Stegun,F.W.; Cirqueira,C.S.; Capelozzi,V.L.
Idiopathic interstitial pneumonias include complex diseases that have a strong interaction between genetic makeup and environmental factors. However, in many cases, no infectious agent can be demonstrated, and these clinical diseases rapidly progress to death. Theoretically, idiopathic interstitial pneumonias could be caused by the Epstein-Barr virus, cytomegalovirus, adenovirus, hepatitis C virus, respiratory syncytial virus, and herpesvirus, which may be present in such small amounts or such configuration that routine histopathological analysis or viral culture techniques cannot detect them. To test the hypothesis that immunohistochemistry provides more accurate results than the mere histological demonstration of viral inclusions, this method was applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrosing interstitial pneumonia; Viral infections; Immunohistochemistry; Tissue microarray.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100985

Effect of melatonin and time of administration on irradiation-induced damage to rat testes
Provedor de dados: 56
Autores: Take,G.; Erdogan,D.; Helvacioglu,F.; Göktas,G.; Ozbey,G.; Uluoglu,C.; Yücel,B.; Guney,Y.; Hicsonmez,A.; Ozkan,S.
The effect of ionizing irradiation on testes and the protective effects of melatonin were investigated by immunohistochemical and electron microscopic methods. Eighty-two adult male Wistar rats were divided into 10 groups. The rats in the irradiated groups were exposed to a sublethal irradiation dose of 8 Gy, either to the total body or abdominopelvic region using a 60Co source at a focus of 80 cm away from the skin in the morning or evening together with vehicle (20% ethanol) or melatonin administered 24 h before (10 mg/kg), immediately before (20 mg/kg) and 24 h after irradiation (10 mg/kg), all ip. Caspace-3 immunoreactivity was increased in the irradiated group compared to control (P &lt; 0.05). Melatonin-treated groups showed less apoptosis as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Testes; Gamma-irradiation; Melatonin; Immunohistochemistry; Electron microscopy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700006

Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody
Provedor de dados: 56
Autores: Pizarro,C.B.; Oliveira,M.C.; Coutinho,L.B.; Ferreira,N.P.
Pituitary adenomas sometimes show rapid growth and recurrence, and about one third invade the structures surrounding the sella turcica. In an attempt to determine aggressive behavior at an early stage, we used the MIB-1 antibody to identify the Ki-67 antigen. The present study was designed to evaluate pituitary adenomatous tissue in terms of secretion and proliferation and to correlate the Ki-67 index with hormone phenotype and invasive behavior. Material from 159 patients submitted to one or more resections of pituitary adenomas was evaluated. Forty-two non-secretory adenomas and 43 adenomas immunoreactive for growth hormone, 19 for prolactin, 18 for growth hormone and prolactin, 16 for adrenocorticotropic hormone (ACTH), and 21 cases of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary adenoma; Invasive adenoma; Ki-67 antigen; MIB-1 antibody; Cell proliferation; Immunohistochemistry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200011

Distribution of calcium-binding proteins in the chick visual system
Provedor de dados: 56
Autores: Pfeiffer,C.P.; Britto,L.R.G.
The calcium-binding proteins calbindin (CB), calretinin (CR), and parvalbumin (PV) have been extensively studied over the last decade since they appear to be important as buffers of intracellular calcium. In the present study we investigated the distribution of these proteins in the chick visual system by means of conventional immunocytochemistry. The results indicated that CB, CR, and PV are widely distributed in retinorecipient areas of the chick brain. In some regions, all three calcium-binding proteins were present at different intensities and often in different neurons such as in the dorsolateral thalamic complex. In other areas, such as the nucleus geniculatus lateralis ventralis, only CB and CR were detected, whereas PV was absent. These results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calbindin; Calretinin; Chick; Immunohistochemistry; Parvalbumin; Visual system.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100009

Abundant immunohistochemical expression of dopamine D2 receptor and p53 protein in meningiomas: follow-up, relation to gender, age, tumor grade, and recurrence
Provedor de dados: 56
Autores: Trott,G.; Pereira-Lima,J.F.S.; Leães,C.G.S.; Ferreira,N.P.; Barbosa-Coutinho,L.M.; Oliveira,M.C.
Meningiomas are common, usually benign tumors, with a high postoperative recurrence rate. However, the genesis and development of these tumors remain controversial. We aimed to investigate the presence and implications of a mutated p53 protein and dopamine D2 receptor in a representative series of meningiomas and to correlate these findings with age, gender, tumor grade, and recurrence. Tumor tissue samples of 157 patients diagnosed with meningioma (37 males and 120 females, mean age 53.6±14.3 years) who underwent surgical resection between 2003 and 2012 at our institution were immunohistochemically evaluated for the presence of p53 protein and dopamine D2 receptor and were followed-up to analyze tumor recurrence or regrowth. Tumors were classified as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Receptors; Dopamine D2; Immunohistochemistry; Meningioma; Tumor suppressor protein p53; Recurrence.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500415

Expression of merlin, NDRG2, ERBB2, and c-MYC in meningiomas: relationship with tumor grade and recurrence
Provedor de dados: 56
Autores: Ongaratti,B.R.; Silva,C.B.O.; Trott,G.; Haag,T.; Leães,C.G.S.; Ferreira,N.P.; Oliveira,M.C.; Pereira-Lima,J.F.S.
Meningiomas are common, usually benign tumors of the central nervous system that have a high rate of post-surgical recurrence or regrowth. We determined expression of the proteins merlin, NDRG2, ERBB2, and c-MYC in meningiomas using immunohistochemistry and assessed relationships between protein expression and gender, age, tumor grade, and recurrence or regrowth. The study sample comprised 60 patients, (44 women and 16 men) with a mean age of 53.2±12.7 years. Tumors were classified as grade I (n=48) or grades II and III (n=12). Expression of merlin, NDRG2, ERBB2, and c-MYC was not significantly different statistically with relation to gender, age, or meningioma recurrence or regrowth. Merlin was expressed in 100% of the cases. No statistically significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meningioma; Merlin; NDRG2; ERBB2; C-MYC; Immunohistochemistry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400706

Vascular dysfunction by myofibroblast activation in patients with idiopathic pulmonary fibrosis and prognostic significance
Provedor de dados: 56
Autores: Parra,E.R.; Falzoni,R.; Capelozzi,V.L.
In this study, we demonstrated the importance of telomerase protein expression and determined the relationships among telomerase, endothelin-1 (ET-1) and myofibroblasts during early and late remodeling of parenchymal and vascular areas in usual interstitial pneumonia (UIP) using 27 surgical lung biopsies from patients with idiopathic pulmonary fibrosis (IPF). Telomerase+, myofibroblasts α-SMA+, smooth muscle cells caldesmon+, endothelium ET-1+ cellularity, and fibrosis severity were evaluated in 30 fields covering normal lung parenchyma, minimal fibrosis (fibroblastic foci), severe (mural) fibrosis, and vascular areas of UIP by the point-counting technique and a semiquantitative score. The impact of these markers was determined in pulmonary functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Idiopathic pulmonary fibrosis; Vascular activity; Immunohistochemistry; Survival.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700015

A poor prognosis in human hepatocellular carcinoma is associated with low expression of DPP4
Provedor de dados: 56
Autores: Yu,Hao; Mei,Xiao-Ping; Su,Peng-Fei; Jin,Guang-Zhi; Zhou,Hong-Kun.
This study aimed to explore the prognostic role of dipeptidyl peptidase 4 (DPP4) expression in hepatocellular carcinoma (HCC). DPP4 expression was measured in formalin-fixed paraffin-embedded specimens that were gathered from 327 HCC patients. Immunohistochemistry analyses were utilized to examine DPP4 expression characteristics and prognostic values (overall survival (OS) and time to recurrence) of DDP4 in HCC tissues. In addition, a patient-derived xenograft (PDX) model was used to assess the correlation between DPP4 expression and tumor growth in vivo. DPP4 was expressed in low levels in HCC tissues in contrast to paired peritumoral tissues (38 cases were down-regulated in a total of 59 cases, 64.4%. P=0.0202). DPP4 expression was significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dipeptidyl peptidase 4; Hepatocellular carcinoma; Immunohistochemistry; X-tile; Overall survival; Patient-derived xenograft.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400609

Chromogenic in situ hybridization compared with other approaches to evaluate HER2/neu status in breast carcinomas
Provedor de dados: 56
Autores: Rosa,F.E.; Santos,R.M.; Rogatto,S.R.; Domingues,M.A.C.
Human epidermal growth factor receptor 2 (HER2) has been evaluated in breast cancer patients to identify those most likely to benefit from herceptin-targeted therapy. HER2 amplification, detected in 20-30% of invasive breast tumors, is associated with reduced survival and metastasis. The most frequently used technique for evaluating HER2 protein status as a routine procedure is immunohistochemistry (IHC). HER2 copy number alterations have also been evaluated by fluorescence in situ hybridization (FISH) in moderate immunoexpression (IHC 2+) cases. An alternative procedure to evaluate gene amplification is chromogenic in situhybridization (CISH), which has some advantages over FISH, including the correlation between HER2 status and morphological features....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CISH; Breast cancer; HER2; Immunohistochemistry; FISH.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300207

Leptospires detection in kidney, liver and uterus of cows slaughtered in Paraná State, Brazil
Provedor de dados: 58
Autores: Silva,Francielle Gibson da; Freitas,Julio Cesar de; Anzai,Eleine Kuroki; Hashimoto,Vanessa Yumi; Giraldi,Nilson; Delbem,Ádina Cléia B.; Bracarense,Ana Paula F.R.L.; Reis,Antônio Carlos F. dos; Vasconcellos,Silvio Arruda.
The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14), from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct examination detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospire; Indirect immunoperoxidase; Immunohistochemistry; Cows; Culture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100008

Cholangiocarcinoma in an American Rhea (Rhea Americana araneipes)
Provedor de dados: 65
Autores: Rocha,Paulo Ricardo Dell'Armelina; Lopes,Letícya Lerner; Arruda,Laura Peixoto de; Pescador,Caroline Argenta; Cruz,Raquel Aparecida Sales da; Colodel,Edson Moleta.
Cholangiocarcinomas are neoplasms that originate from the bile duct epithelium. The present case described a cholangiocarcinoma in an adult female American Rhea (Rhea Americana araneipes) by means of gross, histopathology and immunohistochemistry. Irregular, firm, multifocal, yellow-white masses, measuring from 0.4 to 6cm in diameter were observed in both liver lobes. At the cut surface, multiple firm nodules filled with connective tissue were present. Microscopically, the neoplasia was composed of small, irregular, gland-like structures of neoplastic cells surrounded by connective tissue. The cells resembled epithelial cells of the hepatic biliary ducts. Neoplastic cells were positive for cytokeratin and negative for vimentin. This is the first report of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholangiocarcinoma; Rhea; Immunohistochemistry; Wildlife.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000200284

Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment
Provedor de dados: 65
Autores: Laskoski,Luciane Maria; Valadão,Carlos Augusto Araújo; Vasconcelos,Rosemeri de Oliveira; Ferrucci,Danilo; Silva,Juliete Aparecida Francisco; Machado,Dagmar Ruth Stach; Doria,Renata Gebara Sampaio; Mendonça,Fabio de Sousa.
Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laminitis; Metalloprotease; Horse; Zymography; Immunohistochemistry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100012

Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
Provedor de dados: 65
Autores: Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
ABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine visceral leishmaniasis; Structural changes; Genital pathology; Immunohistochemistry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641

Ancillary techniques on the evaluation of canine cutaneous mast cell tumors from Brazil
Provedor de dados: 65
Autores: Flores,Mariana Martins; Mazaro,Renata Dalcol; Langohr,Ingeborg Maria; Roy,Alma; Strother,Keith; Fighera,Rafael Almeida.
ABSTRACT: The use of histologic classification by a 2-tier grading system only, immunohistochemistry (IHC) for KIT and Ki-67 and polymerase chain reaction (PCR) for internal tandem duplications (ITD) on exon 11 has improved the prognostication of canine cutaneous mast cell tumors (CCMTs) particularly in the United States. However, these techniques are not commonly used in most Brazilian laboratories. Likewise, no studies, to date, have investigated the occurrence of ITD in CCMTs from the country. Thus, this study tested the 2-tier grading system, the immunohistochemistry for KIT and Ki-67 and the PCR for exon 11 in a group of Brazilian CCMTs with the goal of investigating the applicability of these tests in a Brazilian laboratory. Of the 39 CCMTs, 69.2%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunohistochemistry; Ki-67; Mutations; PCR; Mast cell tumor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001804

Myelopathy associated with cervical epidural spinal metastasis of follicular-compact thyroid carcinoma in two dogs
Provedor de dados: 65
Autores: Tochetto,Camila; Rosa,Fábio Brum; Santos,Rosmarini Passos dos; Mazzanti,Alexandre; Fighera,Rafael Almeida; Kommers,Glaucia Denise.
This case report describes the clinical, histopathological and immunohistochemical findings in two dogs with myelopathy associated with metastasis of follicular-compact thyroid carcinoma. Microscopically, both primary neoplasms were characterized by polygonal cells arranged in follicles (occasionally filled with eosinophilic colloid-like material) or in sheets. The neoplastic cells had a moderately eosinophilic cytoplasm and there was moderate anisokaryosis and anisocytosis. Additionally, the neoplastic cells were positive for thyroglobulin on immunohistochemistry, confirming the follicular origin of both tumors. This is a very uncommon presentation of this pathological condition.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunohistochemistry; Oncology; Thyroid tumor; Neurooncology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801487

Correlation between chronic inflammation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum
Provedor de dados: 65
Autores: Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
ABSTRACT: The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with  Leishmania infantum . Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine visceral leishmaniasis; Genital pathology; Immunohistochemistry; QPCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001102029

Spinal cord anaplastic meningioma with extra-neural metastasis in a cat
Provedor de dados: 65
Autores: Prates,Klaus Scherer; Bianchi,Matheus Viezzer; Mello,Lauren Santos de; Matesco,Viviana Cauduro; Lorenzo,Cíntia de; Driemeier,David; Pavarini,Saulo Petinatti.
ABSTRACT:  Meningiomas are the most frequent neoplasms involving the brain in dogs and cats, and are occasionally observed in the spinal cord. They cause compression of the central nervous system; however, do not infiltrate the neuropile and rarely metastasize to other organs. The present study describes a case of anaplastic spinal meningioma with extra neural metastasis in a 20 years-old cat. Clinically, the feline presented a clinical history of 120 days with paresis of the hind limbs and loss of the tail’s movements, with subsequent death. At necropsy, there was an irregular and soft whitish mass involving the meninges from the lumbar intumescence to the sacral region of the spinal cord. Similar white nodular masses were observed in the lungs, liver and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ataxia; Immunohistochemistry; Spinal cord; Meningioma; Metastasis; Central nervous system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700550

Cardiac rhabdomyoma in a slaughtered pig
Provedor de dados: 65
Autores: Pereira,Paula Reis; Cruz,Raquel Aparecida Sales da; Jühlich,Luciane Maria; Sonne,Luciana; Barcellos,David Emílio Santos Neves de; Driemeier,David.
ABSTRACT:  Rhabdomyoma is a rare neoplasm of striated muscle that occurs predominantly in the myocardium. In animals, cardiac rhabdomyoma are observed as incidental lesions in slaughter pigs and have been rarely described in other species, such as cattle, dogs, and deer. This report describes a case of cardiac rhabdomyoma in a male pig at slaughter age that died suddenly in the pre-slaughter period. At necropsy, multiple nodules were observed in the right and left ventricular walls and the interventricular septum. Histopathological examination showed neoplastic proliferation composed of polyhedral cells, with vacuolated cytoplasm that sometimes presented a “spider cell” appearance. Histochemical staining with Schiff’s periodic acid revealed glycogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; Cardiac tumor; Glycogen; Spider cell; Immunohistochemistry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000553

Facial nerve ganglioneuroblastoma in a feline leukemia virus-positive cat
Provedor de dados: 65
Autores: Pereira,Paula Reis; Tagliari,Nelson Junior; Leite-Filho,Ronaldo Viana; Schaefer,Gabriela da Cruz; Costa,Fernanda Vieira Amorim da; Pavarini,Saulo Petinatti.
ABSTRACT: Neuroblastic tumors can originate from the central neuraxis, olfactory epithelium, adrenal medullary region or autonomous system. Ganglioneuroblastoma are a type of neuroblastic tumor, with very few case descriptions in animals. Diagnosis of facial nerve ganglioneuroblastoma was made in a feline leukemia virus-positive 11-month-old cat. The cat had hyporexia, left head tilt, depressed mental state, horizontal nystagmus, inability to retract the pinched left lip, anisocoria, ptosis, and absence of the menace reflex. Gross necropsy showed a mass at the left facial nerve root region. Histological examination of this mass showed neoplastic proliferation of neuroblasts arranged in a cohesive pattern and mature ganglion cells. Ganglion cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuronal neoplasm; Cranial nerve VII; Immunohistochemistry; Feline leukemia virus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500551

A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil
Provedor de dados: 65
Autores: Pires,Prhiscylla Sadanã; Ecco,Roselene; Silva,Rodrigo Otávio Silveira; Araújo,Marina Rios de; Salvarani,Felipe Masiero; Heneine,Luíz Guilherme Dias; Oliveira Júnior,Carlos Augusto de; Lobato,Francisco Carlos Faria.
ABSTRACT: A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) samples tested. Clostridium chauvoei and Clostridium perfringens type A were the most common agents found alone, being detected in ten (26.3%) and six (15.8%) samples, respectively. The other cases showed an association of two or three clostridia, with C. perfringens type A detected in 11 (29%) cases. Based on the findings of the present study, polyvalent vaccines against clostridial infections of animals incorporating C. perfringens would be more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gas gangrene; Blackleg; Malignant edema; Cattle; Sheep; Immunohistochemistry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100452

Immunohistochemical expression of B and T-lymphocytes and TGF-β in experimentally transplanted canine venereal tumor
Provedor de dados: 65
Autores: Silveira,Ana Carolina Trompieri; Gerardi,Daniel; Mouro,Juliana Vitti; Costa,Mirela Tinucci; Alessi,Antonio Carlos.
The tumor/host relationship may have a determining role in the progression or remission of a tumor. Greater infiltration of leukocytes into tumors has been associated with a better prognosis, although controversy regarding whether these cells have a central role in antitumor immunity still exists. Canine transmissible venereal tumor (TVT) is an experimentally transplantable type of tumor that has been used as an experimental model for the tumor/host relationship. The aim of this study was to evaluate the infiltration of T-lymphocytes (CD3, CD4, CD8) and B lymphocytes (CD79-α) and the expression of the cytokine TGF-β in TVT, by means of immunohistochemistry (ABC method). The experimental tumors were composed of puppies that developed TVT after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocytes; TVT; Immunohistochemistry; Dog.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000400029

Vaginal leiomyosarcoma in a cow from Uberlândia, Minas Gerais, Brazil
Provedor de dados: 65
Autores: Saut,João Paulo Elsen; Oliveira,Patrícia Magalhães de; Nasciutti,Nayara Resende; Medeiros,Alessandra Aparecida; Magalhães,Geórgia Modé; Tsuruta,Suzana Akemi; Hanna,Paul; Headley,Selwyn Arlington.
The clinical, pathological, and immunohistochemical findings associated with a vaginal leiomyosarcoma in a 12-year-old, Senepol breed of cow from Uberlândia, Minas Gerais are described. The animal had a large ulcerative neoplastic growth that originated between the base and the left-lateral vaginal wall. Histopathology revealed a tumor formed by muscle-like cells that demonstrated cellular pleomorphism, anisokaryosis, prominent and multiple nucleoli, with rare tumor giant cells. The neoplastic growth invaded adjacent adipose tissue, and contained areas of hemorrhage with discrete accumulations of inflammatory cells. By immunohistochemistry, most neoplastic cells expressed actin, while immunoreactivity to desmin was weakly expressed. These findings support...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine diseases; Immunohistochemistry; Reproductive pathology; Smooth muscle tumor.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000500024

Myolipoma associated with hyperadrenocorticism and diabetes mellitus in a female poodle
Provedor de dados: 65
Autores: Pöppl,Alan Gomes; Beck,Carlos Afonso de Castro; Fonini,Alessandra Van Der Laan; Cruz,Leonardo Montagna; Pavarini,Saulo Petinati.
ABSTRACT:  This report provides an unpublished account of an intra-abdominal myolipoma in a 11-year-old female Poodle with hyperadrenocorticism and diabetes mellitus. The patient was well controlled for both endocrine diseases; however, was suffering for severe abdominal discomfort. Ultrasound scan revealed a huge abdominal mass. At necropsy a pinkish mass (35cm x 28cm x 12cm) of elastic consistency attached to the broad ligament of the left uterine horn was reported. Histologically, this mass was constituted of a mixed population of well-differentiated adipocytes and of spindle-shaped mesenchymal cells. Both tumor cells were positive for vimentin at immunohistochemistry, while the spindle-shaped cells were positive for desmin and actin, compatible with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Abdominal tumors; Cushing’s syndrome; Immunohistochemistry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000550

Anti-Sarcocystis neurona immunostaining associated with equine protozoal myeloencephalitis in Brazil
Provedor de dados: 65
Autores: Paixão,Tatiane Alves da; Rêgo,Isabela Oliveira de Paula; Santos,Renato de Lima.
A retrospective study (1942 to 2005) of histopathological lesions included samples of central nervous system (SNC) from 203 animals in the Equidae family. A total of 42.4% of these samples had significant pathological changes, which were classified as inflammatory (62.8%), degenerative (25.6%), circulatory (10.5%), and neoplasic (1.1%) lesions. Immunohistochemistry anti-Sarcocystis neurona antigens was performed in all the cases with inflammatory changes (54), of which one of the case of encephalitis resulted positive to immunostaining. Although evidence of EPM (Equine Protozoal Myeloencephalitis) has been previously reported in Brazil, to the best of our knowledge, this is the first report in which characteristic EPM lesion was associated with anti-S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Sarcocystis neurona; Myeloencephalitis; Immunohistochemistry; Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600052

Pythium insidiosum colitis in a dog: treatment and clinical outcome
Provedor de dados: 65
Autores: Fujimori,Mahyumi; Lopes,Erika Rondon; Lima,Samara Rosolem; Paula,Daphine Ariadne Jesus de; Almeida,Arleana do Bom Parto Ferreira de; Colodel,Edson Moleta; Pescador,Caroline Argenta; Néspoli,Pedro Eduardo Brandini; Nakazato,Luciano; Dutra,Valéria; Souza,Roberto Lopes de; Sousa,Valéria Régia Franco.
ABSTRACT: The aim of this report is to describe the clinical, pathological and imaging findings and treatment of colitis caused by Pythium insidiosum in a canine presenting haematochezia and progressive weight loss. Through imaging, a thickening of the transverse and descending colon was observed. Histopathological analysis of the large intestine fragment revealed the presence of hyphae, confirmed by immunohistochemistry and PCR as P. insidiosum. Antifungal treatment with itraconazole implemented after partial surgical resection, resulted in control of the disease.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal pythiosis; PCR; Immunohistochemistry; Canine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300526

Cholesteatoma and hydrocephalus associated to a third ventricle meningioma in a cat
Provedor de dados: 65
Autores: Wentz,Maria Fernanda; Vargas,Thainã Piccolo; Bianchi,Matheus Viezzer; Fett,Rochana Rodrigues; Lorenzo,Cíntia De; Pavarini,Saulo Petinatti.
ABSTRACT:  Meningiomas are neoplasms that commonly involve the central nervous system of cats, while cholesteatomas are nodular granulomatous chronic lesions within the choroid plexus that are rarely reported in cats. This study described a case of cholesteatoma and non-communicating hydrocephalus associated to a third ventricle meningioma in a cat. Clinically, the cat had a 2-year history of behavioral changes, photophobia and motor incoordination. At the necropsy, a tan-brown mass totally occluded the third ventricle, causing a severe dilation of the lateral ventricles (non-communicating hydrocephalus). Microscopically, the mass was composed by a neoplastic proliferation of spindle cells arranged in bundles, containing in the center psammomatous bodies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-communicating hydrocephalus; Immunohistochemistry; Intraventricular tumors; Domestic cats.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001000551

Rabies outbreak in buffaloes in Rio Grande do Sul, Brazil
Provedor de dados: 65
Autores: Bianchi,Ronaldo Michel; Panziera,Welden; Galiza,Glauco José Nogueira de; Kommers,Glaucia Denise; Fighera,Rafael Almeida.
ABSTRACT: This report aimed to describe the clinical, epidemiological and pathological aspects of an outbreak of rabies in buffaloes in Rio Grande do Sul (RS), Brazil. Seven out of 41 buffaloes from the same farm became fatally ill; they showed the clinical signs of ataxia, ascending paresis, and paralysis of the limbs. During necropsy, marked hyperemia of the leptomeningeal vessels, and severe distention of the urinary bladder were noted. Histologically, there were indications of nonsuppurative meningoencephalitis and ganglioneuritis with intracytoplasmic and eosinophilic inclusion bodies in the neurons. The fluorescent antibody test was positive for rabies. Viral antigens were also detected by immunohistochemistry (IHC) in sections of the cerebellum....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffaloes; Rabies virus; Brazil; Immunohistochemistry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000400551

Aortic body tumors in dogs from Northern Paraná, Brazil
Provedor de dados: 65
Autores: Headley,Selwyn Arlington; Bracarense,Ana Paula; Nakagawa,Tizianne; Moreno,Kleber; Pereira,Patrícia Mendes; Reis,Antônio Carlos Faria dos.
This article describes the occurrence of aortic body tumors in four dogs from Southern Brazil. All dogs were males, more than 9 year of age; most of these were from the Boxer breed of dogs. The animals had large neoplastic growths at the base of the heart, which demonstrated histologic characteristics consistent with that of aortic body tumor. By immunohistochemistry, tissue sections from all dogs reacted positively to neuron specific enolase. These cases might represent the first description of this tumor in dogs from Brazil, as confirmed by immunohistochemistry.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Heart; Aortic body tumor; Immunohistochemistry; Neuron specific enolase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600045

Cyclooxygenase-2 expression in epithelial neoplasms and its relevance as a targeted therapy in dogs
Provedor de dados: 65
Autores: Cunha,Rúbia Monteiro de Castro; Horta,Rodrigo dos Santos; Lavalle,Gleidice Eunice; Araújo,Roberto Baracat.
ABSTRACT: Targeted therapy of neoplasms is an emergent approach in human and veterinary medicine. Cyclooxygenase (COX) is a class of catalytic enzymes related to the formation of inflammatory mediators. COX-2 is expressed constitutively in a few body tissues, but it may be induced in specific pathophysiologic conditions, such as cancer. COX-2 expression in neoplams may be considered a potential predictive factor, due to the possible association of selective COX-2 inhibitors in adjuvant treatments. This scientific communication has the objective to report COX-2 expression in seven neoplasms of dogs and the usage of adjuvant treatment with COX-2 selective inhibitors as an effective and feasible option in cancer treatment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Carcinoma; Immunohistochemistry; Antineoplastic drugs.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000601050

Carcinoid of the gallbladder in two dogs
Provedor de dados: 65
Autores: Argenta,Fernando Froner; Pereira,Paula Reis; Bertolini,Marianna; Fratini,Letícia Mendes; Saccaro,Renata de Oliveira; Sonne,Luciana; Driemeier,David; Pavarini,Saulo Petinatti.
ABSTRACT: A carcinoid is a rare neoplasm derived from neuroendocrine cells that may arise in the hepatobiliary system. This report described two cases of carcinoid of the gallbladder in dogs, with emphasis on the clinical, pathological and immunohistochemical aspects. Case 1 was a 10-year-old female Shih Tzu, with a history of prostration and occasional episodes of diarrhea and vomiting. Case 2 was a 10-year-old male Cocker Spaniel, with a history of diarrhea and abdominal pain. Abdominal ultrasonography suggested a neoplasm of the gallbladder in both cases. The dogs underwent cholecystectomy, and 10% formalin-fixed samples were sent for pathological examination. Histologically, a neoplastic proliferation of neuroendocrine cells was observed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine; Neoplasm; Neuroendocrine carcinoma; Immunohistochemistry.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200551

Histopathological findings and detection of parasites in the eyes of dogs infected naturally with Leishmania chagasi
Provedor de dados: 65
Autores: Brito,Fábio Luiz da Cunha; Laus,José Luiz; Tafuri,Wagner Luiz; Figueiredo,Maria Marta; Silva Júnior,Valdemiro Amaro; Maia,Frederico Celso Lyra; Alves,Leucio Câmara.
The objective of the present study was to investigate the histopathological alterations and detection of parasites that occur in the eyes of dogs naturally infected with Leishmania chagasi. The eyes of 25 dogs with a positive parasitological and serological exam for L. chagasi were submitted to routine ophthalmic examination. Fragments were obtained from the eyeball and were analyzed by immunohistochemistry and histopathology. Among the structures studied, the conjunctiva of the third eyelid was the tissue most frequently stained for L. chagasi. Histopathological analysis revealed a predominantly mononuclear inflammatory infiltrate whose intensity ranged from discrete to intense in the various ocular structures. Vascular congestion and perivasculitis were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine; Leishmaniasis; Ocular abnormalities; Histopathology; Immunohistochemistry.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000500022

Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis
Provedor de dados: 64
Autores: Venâncio,Vinícius Paula; Ferreira,Eric Batista; Brigagão,Maísa Ribeiro Pereira Lima; Paula,Fernanda Borges de Araújo; Barbisan,Luis Fernando; Azevedo,Luciana.
A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberrant crypt foci; Brazilian savannah; Dimethylhydrazine; Immunohistochemistry; Oxidative stress.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008