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Sensitive spectrophotometric assay of simvastatin in pharmaceuticals using permanganate
Provedor de dados: 60
Autores: Tharpa,Kalsang; Basavaiah,Kanakapura; Rajedraprasad,Nagaraju; Vinay,Kanakapura Basavaiah; Hiriyanna,Salmara Ganeshbhat.
Two simple, sensitive, selective and inexpensive spectrophotometric methods are described for the determination of simvastatin (SMT) in bulk drug and in tablets using permanganate as the oxidimetric reagent. In method A, SMT is treated with a measured excess of permanganate in acetic acid medium and the unreacted oxidant is measured at 550 nm, whereas in method B the reaction is carried out in alkaline medium and the resulting manganate is measured at 610 nm. In method A, the amount of permanganate reacted corresponds to the SMT content and the absorbance is found to decrease linearly with the concentration; and in method B, the absorbance increases with concentration. The working conditions of assays were optimized, and the methods were validated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simvastatin assay; Spectrophotometry; Permanganate; Pharmaceutical products.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100010

Protective effect of guabiju (Myrcianthes pungens (O. Berg) D. Legrand) and red guava (Psidium cattleyanum Sabine) against cisplatin-induced hypercholesterolemia in rats
Provedor de dados: 60
Autores: Nora,Cleice Dalla; Danelli,Daiane; Souza,Lucéia Fátima; Rios,Alessandro de Oliveira; Jong,Erna Vogt de; Flôres,Simone Hickmann.
The present study was the first to examine the protective effect and antioxidant potential of two fruits native to the south of Brazil, namely guabiju (Myrcianthes pungens (O. Berg) D. Legrand) and red guava (Psidium cattleyanumSabine), with respect to reducing the effects of cisplatin-induced hypercholesterolemia in male Wistarrats. The cisplatin-treated animals showed high glucose, LDL cholesterol, oxidized LDL cholesterol and total cholesterol levels. These native fruits reversed the increase in these parameters. The fat levels in the liver improved with supplementation with the fruits, and there were no differences in the thiobarbituric acid reactive substances (TBARS), creatinine, urea or HDL cholesterol levels, nor in the weight of the organs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guabiju/protective effect; Red guava/protective effect; Myrcianthes pungens (O. Berg) D; Legrand/protective effect; Psidium cattleyanum Sabine/protective effect; Hypercholesterolemia/experimental study; Cisplatin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300483

High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil
Provedor de dados: 60
Autores: Siqueira,Vera Lúcia Dias; Cardoso,Rosilene Fressatti; Pádua,Rubia Andreia Falleiros de; Caleffi-Ferracioli,Katiany Rizzieri; Helbel,Cesar; Santos,Adolfo Carlos Barreto; Aoki,Elisabeth Eyko; Nakamura,Celso Vataru.
In Brazil and other regions of the world, Pseudomonas aeruginosa and Acinetobacter spp. have emerged as important agents of nosocomial infection and are commonly involved in outbreaks. The main objective of the present study was to evaluate the genetic relationship among P. aeruginosa and Acinetobacter spp. isolated from patients in a public university hospital in northwestern Paraná, Brazil, and report their antimicrobial resistance profile. A total of 75 P. aeruginosa and 94 Acinetobacter spp. isolates were phenotypically identified and tested for antibiotic susceptibility using automated methodology. Polymyxin B was tested by disk diffusion for P. aeruginosa. Metallo-β-lactamase (MBL) was detected using a disk approximation test. Genotyping was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa/antimicrobial resistance profile; Pseudomonas aeruginosa/genetic study; Acinetobacter spp./antimicrobial resistance profile; Acinetobacter spp./genetic study; Antimicrobial resistance; Bacterial typing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100006

Kinetics study of hydrochlorothiazide lactose liquid state interaction using conventional isothermal arrhenius method under basic and neutral conditions
Provedor de dados: 60
Autores: Ghaderi,Faranak; Nemati,Mahboob; Siahi-Shadbad,Mohammad Reza; Valizadeh,Hadi; Monajjemzadeh,Farnaz.
ABSTRACT The Maillard reaction of hydrochlorothiazide (HCTZ) and lactose has been previously demonstrated in pharmaceutical formulations. In this study, the activation energy of - hydrohlorothiazide and lactose interaction in the liquid state was ascertained under basic and neutral conditions. Conventional isothermal High Performance Liquid Chromatography (HPLC) technique was employed to ascertain the kinetic parameters using Arrhenius method. Results: The activation energy obtained was 82.43 and 100.28 kJ/mol under basic and neutral conditions, respectively. Consequently, it can be inferred that Maillard reaction is significantly affected by pH, which can be used as a control factor whenever the reaction potentially occurs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrochlorothiazide/lactose/Maillard reaction; Hydrochlorothiazide/lactose/interaction/kinetic parameters; High Performance Liquid Chromatography (HPLC)/kinetic parameters study.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400709

Total flavonoids from Ampelopsis megalophylla suppress proliferation of vascular smooth muscle cells in vivo and in vitro
Provedor de dados: 60
Autores: Qiu,Zhenpeng; Zhou,Junxuan; Hu,Junjie; Wu,Yong; Zheng,Guohua.
ABSTRACT Various benefits of flavonoids for ameliorating cardiovascular diseases have been demonstrated. However, the lowering effects on blood pressure caused by antiproliferative potentials of flavonoids in vascular smooth muscle cells are rare. In this study, the antihypertensive effects of total flavonoids from Ampelopsis megalophylla were investigated. The dynamic pressure values and the rate of media thickness versus lumen diameter were measured by the tail-cuff system and H&amp;E staining in vivo, respectively. The mRNA expressions of ACE, Ang II, eNOS, c-Myc, cyclin D1 and p27Kip1 in thoracic aorta or A7r5 cells were measured by qPCR, respectively. The protein expressions of c-Myc, Cyclin D1, p27Kip1 and β-catenin in tissues or A7r5 cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ampelopsis megalophylla; Flavonoids/effects; Cardiovascular remodeling; Β-Catenin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300619

A conglomeration of preclinical models related to myocardial infarction
Provedor de dados: 60
Autores: Ahsan,Farogh; Mahmood,Tarique; Usmani,Shazia; Bagga,Paramdeep; Shamim,Arshiya; Tiwari,Reshu; Verma,Neeraj; Siddiqui,Mohammed Haris.
Cardiovascular diseases are the main source of death and morbidity in developed and developing nations. Animal models are required to propel our understanding of the pathogenesis, increase our knowledge, disease progress, and mechanism behind cardiovascular disorder, providing new approaches focused to improve the diagnostic and the treatment of these pathological conditions and additionally to test various therapeutic ways to deal with tissue regeneration and re-establish heart working following damage. A perfect model framework ought to be reasonable, effectively controlled, reproducible, and physiologically illustrative of human disease, show cardinal signs and pathology that resembles after the human ailment and ethically stable. The decision of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; Animal model; Isoproterenol; Chemical induced; Ischeamia.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100557

Optimization of a spectrofluorimetric method based on a central composite design for the determination of potassium losartan in pharmaceutical products
Provedor de dados: 60
Autores: Demirkaya-Miloglu,Fatma; Yaman,Mehmet Emrah; Kadioglu,Yucel.
Here, a spectrofluorimetric method for the determination of potassium losartan (PL) in pharmaceutical products is described. The effects of critical parameters, pH, acid molarity, and temperature, on the fluorescence intensity of PL were analyzed, and these parameters were optimized using a central composite design (CCD). The highest fluorescent intensity at excitation (λex) and emission (λem) wavelengths of 248 nm and 410 nm, respectively, was achieved using 0.01 M sulfurous acid (pH 2) at 21.6 °C. Under optimum conditions, the method was linear from 0.025-0.5 µg/mL, with a reasonably high correlation coefficient (0.9993). Furthermore, the method was very sensitive (LOQ, 0.006), accurate (RE, ≤7.06), and precise (%RSD, ≤6.51). After development and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potassium losartan/central composite design; Spectrofluorimetry/quantitative analysis; Pharmaceutical formulations/quantitative analyis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300611

Sequence-based identification of microbial contaminants in non-parenteral products
Provedor de dados: 60
Autores: Senthilraj,Rajapandi; Prasad,Ganduri Sathyanarayana; Janakiraman,Kunchithapatham.
ABSTRACT Phenotypic profiles for microbial identification are unusual for rare, slow-growing and fastidious microorganisms. In the last decade, as a result of the widespread use of PCR and DNA sequencing, 16S rRNA sequencing has played a pivotal role in the accurate identification of microorganisms and the discovery of novel isolates in microbiology laboratories. The 16S rRNA region is universally distributed among microorganisms and is species-specific. Accordingly, the aim of our study was the genotypic identification of microorganisms isolated from non-parenteral pharmaceutical formulations. DNA was separated from five isolates obtained from the formulations. The target regions of the rRNA genes were amplified by PCR and sequenced using suitable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-parenteral products/microbiological contamination; Microbes/identification/non-parenteral products; Phylogenetic analysis/microbial identification; 16S rRNA/use/microbial identification.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200329

Development and evaluation of multiparticulate biphasic system for the treatment of circadian diseases
Provedor de dados: 60
Autores: Martins,Sarah Moherdaui; Müller,Vinicius; Galan,Vanderson; Souza,Fabio Pinheiro de; Andreazza,Itamar Francisco; Rosa,Maurício Ferreira da.
Multiparticulate systems have biopharmaceutical advantages when compared to the monolithic systems, once they allow different patterns of drug release and can be used in different treatments. The aim of the present work was to develop a biphasic controlled release delivery system, using propranolol hydrochloride (PROP) that can be used for the treatment of circadian diseases. This system was obtained by the combination of cellulosic polymers hydroxypropyl methylcellulose (HPMC) and ethylcellulose (EC) in a 2² factorial experimental design, which allowed the optimization of the development stage. The pellets produced and used in biphasic formulations were evaluated for physical and chemical characteristics and presented acceptable results. The immediate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronotherapy; Biphasic Release; Pellets; Cellulosic Polymers; Experimental Design.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400607

In vitro study on the interaction of ketotifen fumarate with anhydrous theophylline
Provedor de dados: 60
Autores: Sayeed,Mohammed Aktar; Habib,Razibul; Rahman,Mominur; Banna,Hasan Al; Rana,Sohel.
The purpose of the present study was to investigate the interaction between ketotifen fumarate and anhydrous theophylline in aqueous media of various pH (1.2 and 6.8). Using Job's continuous-variation analysis and Ardon's spectrophotomeric measurement methods, the values of the stability constants of theophylline with ketotifen were determined at a fixed temperature (37 ºC) at various pH. The stability constants, ranging between 5.66 and 9.92, were derived from Ardon's plot, indicating that comparatively stable complexes had formed as a result of an interaction between the drugs. However, following the interaction of theophylline with ketotifen, stability constants were &lt;1 at gastric pH (1.2) and intestinal pH (6.8). Concurrent administration of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stability constant; Job's method; Ardon's method; Ketotifen; Theophylline.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200004

Polymerase chain reaction and blood culture in blood donors screened by ELISA test for Chagas' disease
Provedor de dados: 60
Autores: Kinoshita-Yanaga,Andréa Tieko; Bértoli,Marta; Martins,Vanessa Aparecida; Mizutani,Angélica Sayuri; Toledo,Max Jean de Ornelas; Araújo,Silvana Marques de; Gomes,Mônica Lúcia.
The objective of this study was to evaluate, through blood culture and PCR, the results of the ELISA for Chagas' disease in the screening of blood donors in the public blood-supply network of the state of Paraná, Brazil, and to map the epidemiological profile of the donors with respect to their risk of infection by Trypanosoma cruzi. The negative and positive results of the ELISA were confirmed by blood culture and PCR for 190/191 individuals (99.5%). For one individual (0.5%), the ELISA was inconclusive, blood culture and IIF were negative, and IHA and PCR positive. Three individuals (1.6%) were positive for T. cruzi on all the tests. Donors were predominantly female, and natives of Paraná, of rural origin, had observed or been informed of the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi/detection; ELISA/use for screening; Blood donors; Blood culture; PCR; Blood bank/screened donors.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100007

Pre-clinical interaction of ayahuasca, a brew used in spiritual movements, with morphine and propofol
Provedor de dados: 60
Autores: Pires,Júlia Movilla; Mendes,Fúlvio Rieli; Pires,Ana Paula Salum; Yonamine,Maurício; Amaral,José Luiz Gomes do; Carlini,Elisaldo Araújo.
ABSTRACT Ayahuasca is a beverage with psychoactive properties used in religious and ceremonial rituals by some religious groups. The main active components of ayahuasca are dimethyltryptamine and the harmala alkaloids with β-carboline structure acting as monoamine oxidase A inhibitors. This combination produces a pronounced activation of serotonergic pathways and presents potential interaction with other psychotropics. The objective of this study was to investigate the possible interactions between ayahuasca and agents employed in general anesthesia. The pharmacological interactions between ayahuasca and morphine or propofol were evaluated in mice using doses of 12, 120 and 1200 mg/kg (0.1 to 10 times the average dose consumed by humans in religious...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ayahuasca; Analgesic effect; Pharmacological interactions; Morphine; Propofol; Β-Carbolines.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100609

Dendrimers as anti-inflammatory agents
Provedor de dados: 60
Autores: Avti,Pramod K.; Kakkar,Ashok.
Dendrimers constitute an intriguing class of macromolecules which find applications in a variety of areas including biology. These hyperbranched macromolecules with tailored backbone and surface groups have been extensively investigated as nanocarriers for gene and drug delivery, by molecular encapsulation or covalent conjugation. Dendrimers have provided an excellent platform to develop multivalent and multifunctional nanoconjugates incorporating a variety of functional groups including drugs which are known to be anti-inflammatory agents. Recently, dendrimers have been shown to possess anti-inflammatory properties themselves. This unexpected and intriguing discovery has provided an additional impetus in designing novel active pharmaceutical agents. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimers; Nanocarriers; Anti-inflammatory agents; Cyclooxygenase (COX-2); Inducible nitric oxide synthase (iNOS); Pro-inflammatory cytokines.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700006

Medication use by adults in greater Manaus: a population-based cross-sectional study, 2015
Provedor de dados: 60
Autores: Lima,Vanessa Gomes; Silva,Marcus Tolentino; Galvão,Taís Freire.
The use of medicines can be an indicator of healthcare access. Our aim was to evaluate the consumption of medicine and associated factors among adults in Manaus Metropolitan Region, located in the north of Brazil. A cross-sectional population-based study was conducted with adults, ≥18 years old, selected by probabilistic sampling. The outcome was the use of medicine in the previous 15 days. Poisson regression with robust variance was used to calculate the prevalence ratio (PR) of medicine consumption, with 95% confidence interval (CI). Use of medicines was reported by 29% (95% CI: 28-31%) of the participants. People with good (PR: 0.82, 95% CI: 0.72-0.94) and fair (PR: 0.77, 95% CI: 0.65-0.90) health status were shown to use less medication than those with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medication use; Cross-Sectional Studies; Adult; Manaus.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100630

Analysis of pharmaceutical assistance in the Taquari Valley, Rio Grande do Sul: profile of service users and access to medication
Provedor de dados: 60
Autores: Kauffmann,Carla; Fernandes,Luciana Carvalho; Deitos,Alícia; Laste,Gabriela; Heberlé,Graziela; Castro,Luís César de; Torres,Iraci Lucena da Silva.
Pharmaceutical assistance concerns actions developed by the pharmacist and other professionals with the purpose of promoting, protecting and restoring health. Medications are an essential tool in this process that focuses on the access to, and the rational use of, drugs. This study is aimed at evaluating the Pharmaceutical Assistance provided by Municipal Health Offices in the Taquari Valley, Rio Grande do Sul. For this purpose, 2,794 users of public pharmacies were interviewed and data was analyzed using descriptive statistics. The users of the public pharmacies were mainly female and individuals with low educational level and income. On average, 2.41 drugs were prescribed per medical prescription and 1.72 of these were procured. Out of the total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical assistance; Unified Health System; Medicines/rational use; Medicines/prescription indicators.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400023

Nesiritide protects endothelial function after balloon-induced trauma in the iliac artery in rabbits
Provedor de dados: 60
Autores: Liu,Shu-Qin; Zhang,Li-Ping; Yan,Huan-Ying; Zhang,Xing-Hua; Wang,Le-Xin.
To assess the effect of nesiritide on the endothelial function of iliac arteries following endothelia trauma. Right iliac artery trauma was created with a balloon catheter. Ten rabbits were treated with a 4-week subcutaneous injection of nesiritide at a fixed daily dose of 0.1mg/kg. Ten rabbits received daily normal saline injection. Plasma endothelin 1 (ET-1), nitric oxide (NO), and Von Willebrand Factor (vWF) were measured before and after the therapies. Tissue proliferating cell nuclear antigen (PCNA) was measured after the treatment. After the treatment, in the therapeutic group, the area under internal elastic membrane and the residual lumen area were higher than in the normal saline group (P &lt;0.05). The plasma levels of ET-1 (91.6±6.8 vs 114.9±6.3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasma endothelin (ET-1); Nitric oxide (NO); Von Willebrand Factor (vWF); Tissue proliferating cell nuclear antigen; Iliac artery.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100568

Ginkgo biloba extract (EGb 761) attenuates oxidative stress induction in erythrocytes of sickle cell disease patients
Provedor de dados: 60
Autores: Furman,Aline Emmer Ferreira; Henneberg,Railson; Hermann,Priscila Bacarin; Leonart,Maria Suely Soares; Nascimento,Aguinaldo José do.
Sickle cell disease promotes hemolytic anemia and occlusion of small blood vessels due to the presence of high concentrations of hemoglobin S, resulting in increased production of reactive oxygen species and decreased antioxidant defense capacity. The aim of this study was to evaluate the protective action of a standardized extract of Ginkgo biloba (EGb 761), selected due to its high content of flavonoids and terpenoids, in erythrocytes of patients with sickle cell anemia (HbSS, SS erythrocytes) subjected to oxidative stress using tert-butylhydroperoxide or 2,2-azobis-(amidinepropane)-dihydrochloride, in vitro. Hemolysis indexes, reduced glutathione, methemoglobin concentrations, lipid peroxidation, and intracellular reactive oxygen species were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginkgo biloba; Oxidative stress; Erythrocytes; Azo compounds; Sickle cell anemia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400009

Evaluation of anti-estrogenic or estrogenic activities of aqueous root extracts of Gunnera manicata L.
Provedor de dados: 60
Autores: Mariotti,Kristiane de Cássia; Schmitt,Gabriela Cristina; Barreto,Fabiano; Fortunato,Rodrigo E.; Singer,Rodrigo B.; Dallegrave,Eliane; Leal,Mirna Bainy; Limberger,Renata Pereira.
Gunnera perpensa (Gunneraceae) is an African plant widely used in traditional medicine. This species is known for its activity involving the female reproductive system, such as inducing or increasing labor, treating female infertility, expelling the placenta and/or preventing post-partum hemorrhage. These properties are probably due to (z)-venusol, a majoritary compound, and its action in conjunction with substances in the whole extract and other natural products. In southern Brazil, a native species Gunnera manicata L. that also belongs to Gunneraceae can be found. In spite of the traditional use of G. perpensa, there is no pharmacological and phytochemical information regarding the South American Gunnera species. Therefore, the aim of this study was to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gunnera manicata L./pharmacognosy; Gunneraceae/pharmacognosy; Medicinal plants; Uterotrophic assay/experimental study.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300018

Protective effects of silymarin on methotrexate-induced damages in rat testes
Provedor de dados: 60
Autores: Yaman,Turan; Uyar,Ahmet; Kaya,Mehmet Salih; Keles,Ömer Faruk; Uslu,Baris Atalay; Yener,Zabit.
Abstract The present study aimed to investigate the protective effects of silymarin (SMN), an antioxidant, on methotrexate (MTX)-induced damage in rat testes. Thirty-two Wistar albino rats were divided into four groups (n = 8): control, MTX (20 mg/kg, i.p. on days 1 and 5), SMN (200 mg/kg, orally), and MTX + SMN (20 mg/kg, i.p. on days 1 and 5 and SMN 200 mg/kg orally) groups. At the end of the 6-week trial period, histopathological, immunohistochemical, biochemical, and spermatological analyses were performed on testes tissues. Histopathologically, MTX-induced damage, including depletion of germ cell and loos of spermatozoa, was significantly improved with SMN treatment. Immunohistochemically, the immunoreactivity of glutathione peroxidase 1 (GPx1) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silymarin/effects; Methotrexate/Testes/induced damages; GPx1; SOD2; Pathology.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100618

Development and validation of an analytical method using High Performance Liquid Chromatography (HPLC) to determine ethyl butylacetylaminopropionate in topical repellent formulations
Provedor de dados: 60
Autores: Pinto,Isadora Cabral; Cerqueira-Coutinho,Cristal; Freitas,Zaida Maria Faria de; Santos,Elisabete Pereira dos; Carmo,Flávia Almada do; Ricci Junior,Eduardo.
ABSTRACT Diseases caused by insects are frequent in poor countries, leading to epidemic scenarios in urban areas; e.g., Dengue, Zika and Chikungunya. For this reason, the development of a safe and efficient topical formulation is essential. Ethyl butylacetylaminopropionate (EB) is a mosquito repellent developed by Merck, which is used in products for adults, children and especially babies, due to its low allergenic potential. The aim of this work was to validate an analytical methodology to quantify EB in a new poloxamer-based formulation by high-performance liquid chromatography (HPLC). The quantification methodology was performed at 40 ºC using a Kromasil reverse-phase column (C18), with the dimensions of 250 x 4.6 mm. The mobile phase was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethyl butylacetylaminopropionate (EB)/quantification; Ethyl butylacetylaminopropionate (EB)/topical formulations; Analytical method; Validation; Repellents; Prophylaxis; High Performance Liquid Chromatography.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200613

Ocotea nutans (Nees) Mez (Lauraceae): chemical composition, antioxidant capacity and biological properties of essential oil
Provedor de dados: 60
Autores: Betim,Fernando Cesar Martins; Oliveira,Camila Freitas de; Souza,Angela Maria de; Szabo,Ellis Marina; Zanin,Sandra Maria Warumby; Miguel,Obdulio Gomes; Miguel,Marilis Dallarmi; Dias,Josiane de Fátima Gaspari.
The present study was undertaken to assess the potential uses of the essential oil obtained from Ocotea nutans (Nees) Mez. The hydrodistilled essential oil from O. nutans leaves was analyzed by gas chromatography-mass spectrometry. Fifty-eight compounds representing 87,29% of the total leaf essential oil components were identified, of which biciclogermacrene(11.41%), germacrene-D (4.89%), bisabolol-11-ol(3.73%) and spathulenol (3.71%) were the major compounds. The essential oil from O. nutans were tested for antibacterial activity using the minimum inhibitory concentracion (MIC) method, Artemia salina method, larvicidal activity in Aedes aegypti, and antioxidant capacity.The antioxidant activity measured by the phosphomolybdenum complex and Prussian blue...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocoteanutans/essential oil/evaluation; Antioxidant capacity; Aedesaegypti; Toxic potential.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100513

Impact Factor: an appropriate criterion for the Qualis journals classification in the Pharmacy area?
Provedor de dados: 60
Autores: Farias,Mareni Rocha; Storb,Bernd Heinrich; Storpirtis,Silvia; Leite,Silvana Nair.
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300101

Chemistry and pharmaceutical sciences always together
Provedor de dados: 60
Autores: Ferreira,Elizabeth Igne.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300001

Prevalence, predictors and outcomes of potential drug-drug interactions in left ventricular failure: considerable factors for quality use of medicines
Provedor de dados: 60
Autores: Haq,Inamul; Ismail,Mohammad; Khan,Fahadullah; Khan,Qasim; Ali,Zahid; Noor,Sidra.
Hospitalized patients with left ventricular failure (LVF) are at high risk for potential drug-drug interactions (pDDIs) and its related adverse effects owing to multiple risk factors such as old age, comorbidities and polypharmacy. This cross-sectional study conducted in two tertiary care hospitals aim to identify frequency, levels and predictors of pDDIs in LVF patients. Data about patients’ demographic, hospital stay, medication therapy, sign/symptoms and laboratory test results were collected for 385 patients with LVF. Micromedex Drug-Reax® was used to screen patients’ medication profiles for pDDIs. Overall prevalence and severity-wise prevalence of pDDIs were identified. Chi-square test was performed for comparative analysis of various variables....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Left ventricular failure; Heart diseases; Potential drug-drug interactions; Drug interactions; Patients safety; Rational drug use.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100589

Development and validation of a rapid high performance liquid chromatography - photodiode array detection method for estimation of a bioactive compound wedelolactone in extracts of Eclipta alba
Provedor de dados: 60
Autores: Kumar,Satyanshu; Dhanani,Tushar.
Following optimization of extraction, separation and analytical conditions, a rapid, sensitive and simple reverse-phase high performance liquid chromatography-photo diode array (HPLC-PDA) method has been developed for the identification and quantification of wedelolactone in different extracts of Eclipta alba. The separation of wedelolactone was achieved on a C18 column using the solvent system consisting of a mixture of methanol: water: acetic acid (95: 5: 0.04) as a mobile phase in isocratic elution mode followed by photo diode array detection at 352 nm. The developed method was validated as per the guidelines of the International Conference on Harmonization (ICH). Calibration curve presented good linear regression (r²&gt;0.998) within the test range and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography/quantitative analysis; High performance liquid chromatography/photo diode array; High performance liquid chromatography/method validation; Eclipta alba/extracts; Eclipta alba/analysis; Wedelolactone/quantification.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100007

Dissolution efficiency and bioequivalence study using urine data from healthy volunteers: a comparison between two tablet formulations of cephalexin
Provedor de dados: 60
Autores: Serra,Cristina Helena dos Reis; Chang,Kyung Hee; Dezani,Thaisa Marinho; Porta,Valentina; Storpirtis,Sílvia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tablets/formulations/bioequivalence study; Cephalexin/dissolution efficiency.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200383

In vitro release of diclofenac diethylamine from gels: evaluation of generic semisolid drug products in Brazil
Provedor de dados: 60
Autores: Goebel,Karin; Sato,Mayumi Eliza Otsuka; Souza,Dayse Fernanda de; Murakami,Fábio Seigi; Andreazza,Itamar Francisco.
In order for the pharmacological action of a topical dermal drug product to occur, the drug must first be released from the vehicle to be available to penetrate the skin layers and reach the site of action. Drug release is mainly dependent on the characteristics of the formulation. Currently, to register a generic or a similar drug product in Brazil performance testing of topical drug products for local action is not required. In this context, this aim of this study was to evaluate the in vitro release of commercial diclofenac diethylamine gel products available on the Brazilian pharmaceutical market, using the vertical diffusion cell method. Factors which may influence the test, such as the type of membrane used, and the effect of the formulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro release test; Diclofenac diethylamine/gel; Synthetic membranes; Generic semisolid drug products.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200003

Safe-by-design development of a topical patch for drug delivery
Provedor de dados: 60
Autores: Vaz,Mafalda. B.; Vitorino,Carla; Sousa,João J. S.
Few topical products have been developed specifically to treat acute and chronic arthritis and inflammation, using non-steroidal anti-inflammatory drugs (NSAIDs). The lack of dosing accuracy commonly found in locally applied semisolid products for cutaneous use is a critical issue that leads to treatment failure. The aim of the present work is to develop a differentiated and innovative topical patch based on a monolithic hydrogel for ibuprofen skin delivery, in order to provide a safer and accurate way of drug administration along with improved treatment compliance. Topical patches based on hydroxypropylmethylcellulose (HPMC) were optimized in composition, in terms of enhancer and adhesive, supported on a systematic assessment of in vitro release and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Topical patches; Ibuprofen; Mechanical properties; Permeation studies; Safe-by-design.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100626

Quercetin: a flavonoid with the potential to treat asthma
Provedor de dados: 60
Autores: Fortunato,Laila Rigolin; Alves,Claudiney de Freitas; Teixeira,Maxelle Martins; Rogerio,Alexandre Paula.
Allergic asthma is a complex inflammatory disorder characterized by airway hyperresponsiveness, eosinophilic inflammation and hypersecretion of mucus. Current therapies include β2-agonists, cysteinyl leukotriene receptor 1 antagonists and corticosteroids. Although these drugs demonstrate beneficial effects, their adverse side effects limit their long-term use. Thus, the development of new compounds with similar therapeutic activities and reduced side effects is both desirable and necessary. Natural compounds are used in some current therapies, as plant-derived metabolites can relieve disease symptoms in the same manner as allopathic medicines. Quercetin is a flavonoid that is naturally found in many fruits and vegetables and has been shown to exert...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quercetin; Flavonoids; Asthma.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400002

Consumption of antihypertensive drugs dispensed under the pharmacy benefit management program
Provedor de dados: 60
Autores: Rocha,Aline Pereira; Santos,Brigitte Rieckmann Martins dos.
Pharmacy benefit management (PBM) programs provide attractive discounts for drug purchase, a relevant measure to address costs, mainly of drugs for the treatment of chronic diseases. This study investigated whether PBM may be used as a tool to provide information about the use of antihypertensive medications when they are purchased. The profile of medicines taken to treat high blood pressure by large IT company employees and their dependents was evaluated from January to December 2009. The mean rate of drug boxes purchased to control hypertension was 9.4 ± 10.0 in 2009. Men purchased more drugs than women. The number of drugs purchased for the treatment of hypertension was lower than expected in all age groups except for individuals aged 54-58 and &gt;59...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Antihypertensive drugs; Drugs/continuous use; Antihypertensive medications/use; Treatment adherence; Drug therapy/adherence to treatment; Medicines/benefit manegement.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400018

Estudos sobre a toxicologia da ε-caprolactama
Provedor de dados: 60
Autores: Bomfim,Marcus Vinicius Justo; Abrantes,Shirley de Mello Pereira; Zamith,Helena Pereira da Silva.
A ε-caprolactama (CAP) é um monômero precursor de polímeros denominados nylon 6. Esses polímeros destinam-se à produção de tapetes, vestuário e materiais plásticos tais como equipamentos, sistemas e componentes automotivos, conectores, além de embalagens plásticas. Resíduos de CAP podem migrar de embalagens plásticas de nylon 6 para os alimentos. Diante disso, foi de interesse realizar uma revisão dos efeitos relativos à exposição à CAP e o seu impacto sobre a saúde humana. Estudos epidemiológicos indicam a possibilidade da CAP causar inflamações oculares e cutâneas, além de irritações no sistema respiratório. Pode ocorrer ainda hipotensão, taquicardia, palpitações, rinorréia, ressecamento nasal, efeitos geniturinários e sobre a reprodução como distúrbios...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprolactama/aspectos toxicológicos; Toxicodinâmica; Mutagenicidade; Carcinogenicidade; Embalagens plásticas/efeito tóxico.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100004

Moisturizing effect of a cosmetic formulation containing pequi oil (Caryocar brasiliense) from the Brazilian cerrado biome
Provedor de dados: 60
Autores: Faria,Wanessa Costa Silva; Damasceno,Gabriel Azevedo de Brito; Ferrari,Márcio.
Caryocar brasiliense, popularly known in Brazil as “pequi”, is a species widely distributed in the Brazilian Cerrado. The seeds are surrounded by a woody endocarp coated with a yellow fleshy mesocarp rich in oil and vitamin A, whose oil has a useful role in the treatment of skin aging and protection of human skin against UV-induced damage and skin hydration. The aim of this study was to evaluate the effect of cosmetic formulations containing pequi oil (Caryocar brasiliense) on skin hydration, after a single application. Hydration effect assessment was performed by applying the formulations under study (Control – no formulation, vehicle, and vehicle + pequi oil) onto forearm skin of 30 human volunteers. Skin capacitance and Transepidermal Water Loss (TEWL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caryocar brasiliense/pharmacognosy; Caryocar brasiliense/cosmetic use; Pequi oil/cosmetic formulations/effect; Skin hydration/use of pequi oil.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100013

Patenting in the cosmetic sector: study of the use of herbal extracts
Provedor de dados: 60
Autores: Magalhães,Wagner Vidal; Baby,André Rolim; Velasco,Maria Valéria Robles; Pereira,Deborah Martin Mendes; Kaneko,Telma Mary.
The aim of this work was to evaluate the innovative performance of herbal extracts applied in cosmetics area, based on information collected from Brazilian, American and European patent banks. Analysis were carried out to evaluate the number of patent deposits from each database, the patent applicants profiles, the companies with most patent applications, and also the main uses of herbal extracts in cosmetics. Based on the results achieved, the number of patents filed at the Brazilian patent bank is much lower than that observed in American and European patent banks. Although the number of patents is limited, the analysis indicated a range of cosmetic applications that acts according to the international market trend, represented by a large number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmetics/patenting; Plant extract/use in cosmetics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400005

Development, stability and in vitro permeation studies of gels containing mometasone furoate for the treatment of dermatitis of the scalp
Provedor de dados: 60
Autores: Salgado,Ana Cristina Gomes Barros; Silva,Alexandra Maria Nunes Nogueira da; Machado,Marta Cristina Jorge Cabral; Duarte,Maria Aida da Silva Costa; Ribeiro,Helena Margarida de Oliveira Marques.
Dermatological inflammatory diseases such as atopic dermatitis, psoriasis and seborrhoeic dermatitis often affect the scalp and the eyebrows. Although there are many dosage forms available, these are particularly critical anatomic regions for application of topical formulations because of the presence of hair. Lotions are therefore the recommended type of drug delivery system for these areas. The presence of hair may limit the application and thus the acceptability of the formulation and its compliance. Because of its low apparent viscosity, lotion application is unpleasant. Gels, given their consistency and adhesiveness, are a suitable alternative to lotions in this situation. The aim of this study was to formulate a stable gel containing mometasone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatitis/treatment; Mometasone furoate; Gel/development; Gels/stability; Skin permeation/in vitro study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100012

Tadalafil-loaded PLGA microspheres for pulmonary administration: preparation and evaluation
Provedor de dados: 60
Autores: Yang,Zhenlei; Wang,Longmei; Tian,Liu; Zhang,Xiuping; Huang,Guihua.
Tadalafil, a long-acting PED-5 inhibitor, is commonly used for the treatment of pulmonary arterial hypertension (PAH). However, its efficacy and clinical application are severely limited by the poor water solubility, low bioavailability and a series adverse effects (e.g. headaches, indigestion). In this study, tadalafil was prepared and loaded into biodegradable PLGA (poly(lactic-co-glycolic acid)) microspheres (TDF-PLGA-MS) via emulsification-solvent evaporation. The resulting microspheres were processed into pulmonary inhalant by freeze drying. The TDF-PLGA-MS was spherical and uniform, with an average particle diameter ~10.29 µm. The encapsulation efficiency and drug loading yield of TDF-PLGA-MS were 81.68% and 8.52%, respectively. The investigation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary arterial hypertension; Tadalafil; PLGA; Microspheres; Pulmonary administration; Micromeritic; In vitro release; Pharmacokinetic; Tissue distribution.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100587

Preparation and in vitro characterization of monoclonal antibody ranibizumab conjugated magnetic nanoparticles for ocular drug delivery
Provedor de dados: 60
Autores: Ayata,Neşe; Sezer,Ali Demir; Bucak,Seyda; Turanlı,Eda Tahir.
Gold coated magnetite nanoparticles were prepared and coated with ranibizumab as an ocular drug delivery system. The surface morphologies of the nanoparticles were determined by Scanning Electron Microscopy (SEM). The size and surface charge were determined by using the dynamic light scattering (DLS) technique. Crystallographic properties of the gold coated Fe3O4 nanoparticles were recorded on X-ray diffractometer (XRD) the XRD pattern of nanoparticlees were shown to have uniqe Fe3O4 and gold peaks. Conjugation of ranibizumab onto nanoparticles was achieved using the physical adsorption method. The amount of ranibizumab on the surface of the nanoparticles was determined by thermogravimetric analysis (TGA). In the in vitro release studies performed using UV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ranibizumab; Monoclonal antibody; Drug delivery; Magnetic nanoparticles; Cell culture.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100587

Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
Provedor de dados: 60
Autores: Almeida,Tamires Cunha; Seibert,Janaina Brandão; Almeida,Sávio Henrique de Souza; Amparo,Tatiane Roquete; Teixeira,Luiz Fernando de Medeiros; Barichello,Jose Mario; Postacchini,Bruna Bueno; Santos,Orlando David Henrique dos; Silva,Glenda Nicioli da.
Antimicrobial and antitumor activities of resveratrol, a compound found mainly in grapes, have already been demonstrated. However, its low bioavailability is a limiting factor for therapeutic application. Polymeric micelles can be an approach to solve this problem since they can encapsulate hydrophobic substances. We developed and characterized micellar formulations containing resveratrol and evaluated their cytotoxic and antimicrobial effects. The formulations were prepared by the cold dispersion method with different concentrations of F127 (5 or 10% w/w) and resveratrol (500 or 5000 µM). The formulations were characterized according to size, polydispersity index, pH, encapsulation rate and in vitro release. Cytotoxic effect was evaluated on a bladder...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial activity; Cytotoxic activity; F127®; Polymeric micelles; Resveratrol.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100567

Reflections on hard and soft technologies applied to health, and their association to the teaching, research, and university outreach activities
Provedor de dados: 60
Autores: Storpirtis,Silvia.
Tipo: Info:eu-repo/semantics/other
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200001

A validated stability indicating HPLC method for the determination of process-related impurities in pantoprazole bulk drug and formulations
Provedor de dados: 60
Autores: Pandey,Saurabh; Pandey,Preeti; Mishra,Durgesh; Singh,Umesh Kumar.
A stability-indicating high-performance liquid chromatographic (HPLC) method was developed with short run time and validated for the assay of process related impurities of pantoprazole in bulk form. Resolution of drug, its potential impurities and degradation products were achieved on a Hypersil ODS column utilizing a gradient with 0.01 M phosphate buffer of pH 7 and acetonitrile as eluent, at the detection wavelength of 290 nm. Flow rate was set at 1 mL min-1. The procedure was found to be specific, linear (r=0.999), recovery (97.9-103%), LOD (0.043-0.047 µgmL-1), LOQ (0.13-0.14 µgmL-1) and robust. Acceptable robustness indicates that the assay method remains unaffected by small but deliberate variations. Pantoprazole was found to degrade in acidic,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography/qualitative analysis; High performance liquid chromatography/method development; Pantoprazole/forced degradation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100019

Eudragit ® FS 30 D polymeric films containing chondroitin sulfate as candidates for use in coating seeking modified delivery of drugs
Provedor de dados: 60
Autores: Reis,Camila Borges dos; Boff,Daniele Schaab; Lima,Isabela Angeli de; Santos,Leandro Freire dos; Calvalcanti,Osvaldo Albuquerque; Bunhak,Élcio José.
ABSTRACT Polymeric films associating different concentrations of Eudragit(r) FS 30 D (EFS) and chondroitin sulfate (CS) were produced by casting for the development of a new target-specific site material. Formed films kept a final polymer mass of 4% (w/v) in the following proportions: EFS 100:00 CS (control), EFS 95:05 CS, EFS 90:10 CS and EFS 80:20 CS. They were analyzed for physical and chemical characteristics using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and Raman spectroscopy. Furthermore, they were characterized by their water vapor permeability and degree of hydration at different conditions simulating the gastrointestinal tract. No chemical interactions were observed between CS and EFS, suggesting only a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymeric films/smelting process; Eudragit(r) FS 30 D/modified delivery; Chondroitin Sulfate; Polymeric coating.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400635

Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity
Provedor de dados: 60
Autores: Arrahman,Arif; Sigalingging,Efendy; Trinovita,Elsa; Saputri,Fadlina Chany; Mun'im,Abdul.
This study investigated the effect of ionic liquid (IL)-microwave assisted extraction (MAE) on γ-oryzanol extraction from rice bran (Oryza sativa L.). The conditions included the concentration of IL, liquid (IL)/solid (sample) ratio, extraction time, and microwave power. The use of 1-butyl-3-methylimidazolium ([Bmim]BF4) as a selected solvent in IL-MAE method was developed for extraction of γ-oryzanol from rice bran. The Box-Behnken design on four factors with response surface methodology (RSM) was used to optimize the experimental conditions. The optimum conditions for the MAE were a [Bmim]BF4 concentration of 0.7 M, liquid/solid ratio of 15 mL/g, extraction time of 10 minutes, and 30% microwave power with a γ-oryzanol value of 0.41 mg/g. The experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: [Bmim]BF4; Ionic liquid; IL-MAE; Γ-oryzanol; Tyrosinase.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100549

A simple method for the quantification of diclofenac potassium in oral suspension by high-performance liquid chromatography with UV-detection
Provedor de dados: 60
Autores: Rubim,Alexandre Machado; Rubenick,Jaqueline Bandeira; Laporta,Luciane Varine; Rolim,Clarice Madalena Bueno.
A rapid, simple and low cost method was developed to determine diclofenac potassium (DP) in oral suspension, using a reverse-phase column (C8, 150 mm x 4.6 mm, 5 µm), mobile phase containing methanol/buffer phosphate (70:30 v/v, pH 2.5), at a flow rate of 1.0 mL/min, isocratic method, and ultraviolet detection at 275 nm. A linear response (r = 1.0000) was observed in the range of 10.0-50.0 µg/mL. Validation parameters such as linearity, specificity, precision, accuracy and robustness were evaluated. The method presented precision (repeatability: relative standard deviation = 1.21% and intermediate precision: between-analyst = 0.85%). The specificity of the assay was evaluated by exposure of diclofenac potassium under conditions of stress such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography; Diclofenac potassium; Oral suspension.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300021

Nephroprotective activity of the enriched polyphenol extract of Euterpe edulis Martius
Provedor de dados: 60
Autores: Cardoso,Priscylla Maria Martins; Mazuco,Renata Alves; Macedo,Lucas Soares Dazzi; Polese,Ariele Abreu Venturini; Barroso,Maria Eduarda de Souza; Baratella,Waléria Gramilich; Andrade,Tadeu Uggere de; Lenz,Dominik; Pereira,Thiago de Melo Costa; Endringer,Denise Coutinho.
Contrast-induced nephropathy (NIC) is directly related to increased morbidity and mortality, and its treatment and prevention might be achieved by the administration of antioxidant products. The juçara palmetto (Euterpe edulis Martius) has fruits rich in phenolic compounds, which are known for their antioxidant activity. This work aimed to evaluate the nephroprotective activity of E. edulis pulp in the NIC animal model. The collected fruits were pulped, their contents of polyphenols and anthocyanins were quantified, and their antioxidant activity were evaluated. The nephroprotective effects were determined based on iodine contrast induction and evaluated by biochemical and histological analyses. The results showed that E. edulis pulp was rich in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nephroprotection; Euterpe edulis Martius; Polyphenols; Anthocyanins; Antioxidant activity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100522

From dendrimers to fractal polymers and beyond
Provedor de dados: 60
Autores: Moorefield,Charles N.; Schultz,Anthony; Newkome,George R.
The advent of dendritic chemistry has facilitated materials research by allowing precise control of functional component placement in macromolecular architecture. The iterative synthetic protocols used for dendrimer construction were developed based on the desire to craft highly branched, high molecular weight, molecules with exact mass and tailored functionality. Arborols, inspired by trees and precursors of the utilitarian macromolecules known as dendrimers today, were the first examples to employ predesigned, 1 → 3 C-branched, building blocks; physical characteristics of the arborols, including their globular shapes, excellent solubilities, and demonstrated aggregation, combined to reveal the inherent supramolecular potential (e.g., the unimolecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arborol; Dendrimer; Supramolecular; Terpyridine; Self-assembly.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700007

Comparison of pharmacopeial statistical methods applied in microbiological assay for antibiotics potency determination using parallel lines and three-dose level
Provedor de dados: 60
Autores: Leonel,Miriam de Fátima Vianna; Soares,Cristina Duarte Vianna; Siqueira,Arminda Lucia.
Pharmaceutical equivalence studies, useful for checking the interchangeability of generic medicines and their respective innovator medicines, have been carried out in Brazil since 1999, as a consequence of the establishment of the generic medicine policy. For medicines containing antibiotics, microbiological assays are often the most appropriate method. However, the statistical methods applied in these assays are not widely known due to the difficult access to official codes and/or little knowledge of the statistical tools of analysis. Thus, the aim of this work was to compare the statistical methods for determining the potency of antibiotics through the cylinder-plate method using parallel lines and a three-dose level model, as described in the Brazilian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics/potency; Parallel lines model; Pharmacopeial statistical methods.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300024

Scientific production in pharmaceutical care: comparison between Brazil, USA and Spain
Provedor de dados: 60
Autores: Andrade,Tadeu Uggere de; Barbosa,Jaqueliny Louback da Cunha; Laignier,Lívia Lopes Moura; Mata,Elizangela Faustino da; Cassaro,Karla Oliveira dos Santos; Lenz,Dominik; Sampaio,Karla Nivea; Boëchat,Giovanna Assis Pereira; Endringer,Denise Coutinho.
The aim of this study was to estimate the evolution of the field of Pharmaceutical Care (PC) by measuring the quality and quantity of the scientific production on the topic of PC in Brazil compared to two pioneering countries in the field, the United States of America (USA) and Spain. The databases Web of Science, Scopus, Medline, Lilacs and SciELO were used as sources for the literature search. Pharmaceutical Care, or the appropriate translations, was used as the search term for the literature search, which was limited to articles published between 1990 and 2009. A score of quality (SQ) was calculated using variables such as impact factor and the frequency of the citations. We included 3265 articles published in 544 journals. We found that there was a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care/scientific production; Brazil/pharmaceutical care; Brazil/scientific production; Spain/pharmaceutical care; Spain/scientific production; United States of America/pharmaceutical care; United States of America/scientific production.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100005

Liquid-liquid extraction combined with high performance liquid chromatography-diode array-ultra-violet for simultaneous determination of antineoplastic drugs in plasma
Provedor de dados: 60
Autores: Sanson,Ananda Lima; Silva,Suéllen Cristina Rennó; Martins,Matheus Coutinho Gonçalves; Giusti-Paiva,Alexandre; Maia,Patrícia Penido; Martins,Isarita.
A liquid-liquid extraction (LLE) combined with high-performance liquid chromatography-diode array detection method for simultaneous analysis of four chemically and structurally different antineoplastic drugs (cyclophosphamide, doxorubicin, 5-fluorouracil and ifosfamide) was developed. The assay was performed by isocratic elution, with a C18 column (5 µm, 250 x 4.6 mm) and mobile phase constituted by water pH 4.0- acetonitrile-methanol (68:19:13, v/v/v), which allowed satisfactory separation of the compounds of interest. LLE, with ethyl acetate, was used for sample clean-up with recoveries ranging from 60 to 98%. The linear ranges were from 0.5 to 100 µg mL-1, for doxorubicin and 1 to 100 µg mL-1, for the other compounds. The relative standard deviations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antineoplastic drugs/simultaneous determination in plasma; Liquid-liquid extraction; High-performance liquid chromatography/diode array detector.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200017

Oleanolic acid from black raisins, Vitis vinifera with antioxidant and antiproliferative potentials on HCT 116 colon cancer cell line
Provedor de dados: 60
Autores: Sasikumar,Kandasamy; Dubey,Vinay; Ghosh,Asit Ranjan.
Vitis vinifera (black raisin) is commonly used in traditional medicine for the treatment of various ailments. In the present study, anti-oxidative and anti-cancer efficacy of oleanolic acid from ethyl acetate fraction of black raisins was evaluated and oleanolic acid was isolated without using of any chromatographic techniques and subjected to spectral assessment using UV-Vis spectrophotometer, 1H NMR, 13C NMR, MS and FT-IR for structural confirmation. Antiproliferative efficacy of oleanolic acid against human colon adenocarcinoma HCT-116 cells was assessed using cell viability assay. The minimum inhibitory concentration (IC50) was determined and found to be 40 µg/mL at 48h incubation. Furthermore, antioxidant property of oleanolic acid was analyzed using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis vinifera; Oleanolic acid; Anti-oxidant; Anti-proliferative.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100502

Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in  Portulaca oleracea L. extract
Provedor de dados: 60
Autores: Xu,Liang; Tao,Xiaojun; Gao,Yucong; Zhang,Wenjie; Meng,Yihan; Li,Cuiyu; Jiang,Mingyue; Xixiang,Ying.
ABSTRACT Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Portulaca oleracea L./hydroxydihydrobovolide; Portulaca oleracea L./hydroxydihydrobovolide/cytotoxicity; Portulaca oleracea L./hydroxydihydrobovolide/pharmacokinetics; Hydroxydihydrobovolide/Ultra-high performance liquid chromatography.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200615

Developed and validated stability indicating HPLC method for the determination of epirubicin in bulk drug, marketed injection and polymeric nanoparticles
Provedor de dados: 60
Autores: Tariq,Mohammad; Thomas,Shindu; Singh,Anu; Talegaonkar,Sushama.
Present work is aimed to develop a simple, sensitive, robust and reliable HPLC method for routine quality control of epirubicin (EPI) in bulk drug, marketed injections and polymeric nanoparticles. Separation was carried out by C18 column. Isocratic elution was carried out using mobile phase A: 0.16% o-phosphoric acid solution, B: acetonitrile and methanol mixture (80:20, v/v) in the ratio of 60:40 (A: B) while the flow rate was maintained at 1mL/min. Analyses were performed at 233.5 nm using PDA detector. Excellent linear relationship was observed between peak-area versus drug concentration in the range of 1.0-100.0 μg/mL (r2, 0.999). Developed method was found to be sensitive (Limits of detection and quantification were found to be ~8 ng/mL and ~25 ng/mL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epirubicin; HPLC; Polymeric nanoparticles; Quality control; Forced degradation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400619

A comparative study of two separate analytical techniques for the simultaneous determination of diclofenac sodium and diacerein from combined dosage form
Provedor de dados: 60
Autores: Mallick,Sanhita Basu; Chattopadhyay,Helen; De,Amit Kumar; Datta,Sriparna.
ABSTRACT Diclofenac sodium (DS) and diacerein (DC) have emerged as a potential combination therapy for the treatment of knee osteoarthritis. Therefore a validated analytical method is essential for the simultaneous estimation of both from combined dosage form. A ratio derivative spectrophotometric and a chromatographic technique have been developed for the simultaneous determination of DS and DC. The quantification was done at 263.00 nm for DC and 304.50 nm for DS in the first method, whereas 257 nm for DC and at 274 nm for DS for LC-DAD analysis in chromatographic method using acetate buffer and methanol as the mobile phase at a flow-rate 0.50 mL/min. Both of these methods are found to be linear in the concentration range under study with r2 value 0.999...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diacerein/comparative study; Diclofenac sodium/comparative study; High Pressure Liquid Chromatography; LC-DAD; Ratio derivative spectroscopy/validation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200601

Dissolution test optimization for meloxicam in the tablet pharmaceutical form
Provedor de dados: 60
Autores: Oliveira,Érika de Fátima Silva; Azevedo,Roberta de Cássia Pimentel; Bonfilio,Rudy; Oliveira,Diego Borges de; Ribeiro,Gislaine Pereira; Araújo,Magali Benjamim de.
Meloxicam is a broadly used drug in the therapeutics for the osteoarthritis and rheumatoid arthritis treatments in adults, and it is available in the Brazilian market, as tablet and capsule pharmaceutical forms. The present work aimed to establish conditions for accomplishment of the dissolution test of 15 mg meloxicam tablets (A and B test products), compared with the reference product, since there is no monograph about dissolution assays for meloxicam in official summaries. To optimize the conditions several parameters were tested and, according to obtained results, the use of pH 7.5 phosphate buffer (900mL, at 37 ± 0.5ºC) as dissolution medium, paddle method (apparatus 2), stirring speed of the dissolution medium at 100 rpm and collect time of 60...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meloxicam/dissolution; Dissolution kinetics; Tablet pharmaceutical forms/dissolution test; Spectrophotometry.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100008

High prevalence rate of extended-spectrum beta-lactamases (ESBL) among Enterobacteriaceae in a small Brazilian public hospital
Provedor de dados: 60
Autores: Lenhard-Vidal,Adriane; Cardoso,Rosilene Fressatti; Pádua,Rubia Andreia Falleiros de; Siqueira,Vera Lúcia Dias.
The production of extended-spectrum beta-lactamases (ESBL) is considered one of the most important resistance mechanisms that impair antimicrobial treatment of infections caused by Enterobacteriaceae. Data on culture and susceptibility tests were collected from the Clinical Analyses and Research Laboratory charts reporting on patients admitted to the University Hospital of Maringá (HUM) from January 2004 to December 2009. The following Enterobacteriaceae were selected: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp. and Proteus mirabilis. All tests were performed according to the recommendations of the Clinical and Laboratory Standards Institute (biochemical identification; susceptibility tests; initial screening and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extended-spectrum β-lactamases; Enterobacteriaceae; Bacterial resistance; Β-lactams.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400006

Prescription of anorectic and benzodiazepine drugs through notification B prescriptions in Natal, Rio Grande do Norte, Brazil
Provedor de dados: 60
Autores: Nappo,Solange Aparecida; Carlini,Elisaldo Araújo; Araújo,Maria Dalva; Moreira,Lúcio Flávio Sousa.
A study was conducted on 22,158 special B prescriptions (notificações B) containing amphetamine-type anorectic drugs or benzodiazepines, obtained from compounding pharmacies or drugstores located in the city of Natal, RN, Brazil. The data obtained were compared with those from other Brazilian cities. Results showed that compounding pharmacies dispensed 85.4% of the prescriptions, indicating that these pharmacies filled out nearly 10 times more of these prescriptions than did the drugstores. The majority (83.5%) of B prescriptions issued for the compounding pharmacies were for women, where the female/male patient ratio ranged from 7.1/1.0 for mazindol to 10.3/1.0 for amfepramone. Similar results were obtained for the benzodiazepines with ratios of 1.9/1.0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anorectic/prescription; Benzodiazepine/prescription; Obesity/treatment; Medicines/rational use; Compounded medicine; Compounding pharmacy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200017

Formulation and characterization of self emulsifing pellets of carvedilol
Provedor de dados: 60
Autores: Bhandari,Vikas; Avachat,Amelia.
The purpose of present study was aimed at developing self emulsifying drug delivery system in liquid and then in pellet form that would result in improved solubility, dissolution and permeability of the poorly water soluble drug carvedilol. Pellets were prepared using extrusion-spheronization technique incorporating liquid SEDDS (carvedilol, capmul MCM EP, cremophore EL, tween 20, propylene glycol), adsorbents ( and crospovidone), microcrystalline cellulose and binder (povidone K-30). Ternary phase diagram was constructed to identify different oil-surfactant-cosurfactant mixtures according to the proportion of each point in it. The optimal CAR-SEDDS pellets showed a quicker redispersion with a droplet size of the reconstituted microemulsion being 160.47...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/self emulsifying/delivery system; Pellets/self emulsifying/preparation; Pellets/self emulsifying/delivery system; Carvedilol/pharmacokinetics.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300663

LC-MS characterization of valsartan degradation products and comparison with LC-PDA
Provedor de dados: 60
Autores: Pires,Sumaia Araújo; Mota,Letícia Martins; Garcia,Jerusa Simone; Amaral,Phellipe Honorio; Meurer,Eduardo César; Eberlin,Marcos Nogueira; Trevisan,Marcello Garcia.
abstract Valsartan was submitted to forced degradation under acid hydrolysis condition as prescribed by the ICH. Degraded sample aliquots were separated via HPLC using a Hypersil ODS (C18) column (250 x 4.6 mm i.d., 5 µm). Either photodiode array (PDA) detection or mass spectrometry (MS) full scan monitoring of HPLC runs were used. HPLC-PDA failed to indicate Valsartan degradation under forced acid degradation, showing an insignificant peak area variation and that Valsartan apparently remained pure. HPLC-MS using electrospray ionization (ESI) and total ionic current (TIC) monitoring did not reveal any peak variation either, but inspection of the ESI mass spectra showed the appearance of m/z 306 and m/z 352 ions for the same retention time as that of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Valsartan; Stress testing; Degradation products; Stability-indicating; Acidic hydrolysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400839

The appropriate use of human albumin in a Brazilian University Hospital: therapeutic indication and dosage regimen
Provedor de dados: 60
Autores: Ishida,Tatiane Sayuri; Sakai,Maria Cristina; Melo,Daniela Oliveira de.
The objective of the study was to evaluate the use of human albumin in a Brazilian university hospital, in compliance with the institutional protocol and other clinical guidelines, taking into account the therapeutic indications and the dosage regimens. Data was obtained from the pharmacy dispensing records of human albumin, the requests for use it and, when available, the patient’s records between January and October 2017. After evaluation the therapeutic indications and the dosage regimens were classified as “appropriate” and “inappropriate”. The analysis of 98 requests showed that, when compared to the institutional protocol, 54 (55.1%) requests had an inappropriate therapeutic indication. However, when a comparison was made between 25 medical records...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albumins/drug utilization review; Health services; University Hospital/Brazil; Drugs/use.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400631

Formulation and evaluation of xanthan gum based aceclofenac tablets for colon targeted drug delivery
Provedor de dados: 60
Autores: Ramasamy,Thiruganesh; Kandhasami,Uma Devi Subbaih; Ruttala,Himabindhu; Shanmugam,Suresh.
The objective of the present study is to develop a colon targeted drug delivery systems for Aceclofenac using xanthan gum as a carrier. In this study, multilayer coated system that is resistant to gastric and small intestinal conditions but can be easily degraded by colonic bacterial enzymes was designed to achieve effective colon delivery of Aceclofenac. The xanthan gum, the drug and the physical mixture were characterized by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). All the formulations were evaluated for hardness, drug content uniformity and other physical properties. Release aspects of Aceclofenac in simulated gastrointestinal fluid and colonic fluid with enzymes were investigated. From these results,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/release colon targeted; Aceclofenac/release colon targeted; Xanthan gum; Colon targeting; Eudragit coating; In vitro dissolution; Targeted delivery.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200011

The production of biopharmaceuticals in Brazil: current issues
Provedor de dados: 60
Autores: Pimenta,Marcela Valente; Monteiro,Gisele.
Biopharmaceuticals are gaining a growing share of the pharmaceuticals market. In recent years, the Brazilian Health Surveillance Agency (ANVISA) has approved the registration of biological drugs with domestic production. Although Brazil is in the early stages of biopharmaceutical production, governmental incentives and the investment in private companies in the technological domain in this country have created expectations of an increase in the capacity of biopharmaceutical production. Private initiatives, once rare, have now started to blossom in this field, such as collagenase from Cristalia and filgrastim from Eurofarm. The expiry of the patents for certain biopharmaceuticals (e.g. infliximab, filgrastim and rituximab) has generated the possibility of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopharmaceutical; Biosimilar; Biological drugs; Brazilian biopharmaceutical market.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100599

Formulation and evaluation of carvedilol microcapsules using Eudragit NE30D and sodium alginate
Provedor de dados: 60
Autores: Bal,Trishna; Sengupta,Shubhranshu; Murthy,Padala Narasimha.
Inclusion complexes of carvedilol(CR) with hydroxyl propyl beta-cyclodextrin (HPBCD) was prepared using co-grinding technique. Then, the inclusion complex was microencapsulated using combinations of Eudragit NE30D (EU) and sodium alginate (SA) utilizing orifice gelation technique. The formulations were analysed by using Scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR), Differential scanning Calorimetry (DSC) and X-ray diffractometer (XRD) and also evaluated for particle size, encapsulation efficiency, production yield, swelling capacity, mucoadhesive properties, zeta potential and drug release. The microcapsules were smooth and showed no visible cracks and extended drug release of 55.2006% up to 12 hours in phosphate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carvedilol/microcapsules; Carvedilol/evaluation; Carvedilol/microcapsules; Carvedilol/formulation; Eudragit NE30D; Orifice Gelation technique.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400029

Antidiabetic activity of aqueous extract of Sigesbeckia orientalis (St. Paul’s Wort) in alloxan-induced diabetes model
Provedor de dados: 60
Autores: Asif,Muhammad; Saleem,Mohammad; Yousaf,Sobia; Saadullah,Malik; Zafar,Memoona; Khan,Rizwan Ullah; Yuchi,Alamgeer.
The current study evaluated antidiabetic and antihyperlipidemic activities of aqueous extract of Sigesbeckia orientalis L. (St. Paul’s Wort) (AESO) in an alloxan-induced diabetic rat model. Following OECD guidelines safe doses of AESO were assessed in rats for the main study. Serum/bood glucose, α-amylase, and lipids levels and histopathological evaluations were conducted to assess antidiabetic and associated antihyperlipidemic efficacies of AESO. AESO was found to be safe up to the dose of 2000 mg/kg. Significant (p &lt; 0.05) reduction in glucose and lipids (total cholesterol, triglycerides, low-density lipoproteins) levels was observed in AESO treatment groups. Serum α-amylase, high-density lipoproteins, and total body weight was increased significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antidiabetic; Sigesbeckia orientalis L; Alloxan; Antihyperlipidemic; HPLC.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100607

Evaluation of Cedrela gum as a binder and bioadhesive component in ibuprofen tablet formulations
Provedor de dados: 60
Autores: Odeniyi,Michael Ayodele; Babalola,Adepeju Oluwadamilare; Ayorinde,John Oluwasogo.
The compressional, mechanical and bioadhesive properties of tablet formulations incorporating a new gum obtained from the incised trunk of the Cedrela odorata tree were evaluated and compared with those containing hydroxypropylmethylcellulose (HPMC). Compressional properties were evaluated using Hausner's ratio, Carr's Index, the angle of repose, and Heckel, Kawakita and Gurnham plots. Ibuprofen tablets were prepared using the wet granulation method. Bioadhesive studies were carried out using the rotating cylinder method in either phosphate buffer pH 6.8 or 0.1 M hydrochloric acid media. The gum is a low viscosity polymer (48 cPs), and Fourier transform infrared spectroscopy revealed the presence of a hydroxyl group. Py and Pk values, which are measures of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cedrela gum/evaluation; Cedrela gum/compression properties; Cedrela gum/bioadhesive component; Tablet formulations; Hydroxypropylmethylcellulose; Ibuprofen tablets/bioadhesive studies; Bioadhesion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100011

Physicochemical and dissolution profile characterization of pellets containing different binders obtained by the extrusion-spheronization process
Provedor de dados: 60
Autores: Issa,Michele Georges; Pessole,Letícia; Takahashi,Andrea Ikeda; Andréo Filho,Newton; Ferraz,Humberto Gomes.
With the purpose of evaluating the behavior of different polymers employed as binders in small-diameter pellets for oral administration, we prepared formulations containing paracetamol and one of the following polymers: PVP, PEG 1500, hydroxypropylmethylcellulose and methylcellulose, and we evaluated their different binding properties. The pellets were obtained by the extrusion/spheronization process and were subsequently subjected to fluid bed drying. In order to assess drug delivery, the United States Pharmacopeia (USP) apparatus 3 (Bio-Dis) was employed, in conjunction with the method described by the same pharmacopeia for the dissolution of paracetamol tablets (apparatus 1). The pellets were also evaluated for granulometry, friability, true density and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Binders/evaluation; Pellets/dissolution profile; Pellets/physicochemical characteristics; Extrusion/spheronization process; Drug delivery/evaluation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300004

Intravenous immunoglobulin therapy among pediatric patients: labeled and off-labeled indications
Provedor de dados: 60
Autores: Toh,Chin Chiang; Islahudin,Farida; Ali,Adli; Shah,Noraida Mohamed.
ABSTRACT This study was designed to evaluate utilization patterns and clinical outcome of intravenous immunoglobulin (IVIG) therapy among pediatric patients in a tertiary hospital. Demographic data, IVIG prescribed, and clinical outcome were retrospectively reviewed from the pharmacy dispensing data and patient medical records between 2007 and 2014. One hundred and fifteen instances of IVIG administration to 108 pediatric patients were recorded. A total of 61 cases (53%) and 54 cases (47%) of the IVIG administered were for labeled and off-labeled indications, respectively. Age, weight, specialty, total IVIG usage, length of hospital stays, and mortality rate were found to be significantly associated with the indication being labeled or off-labeled...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intravenous Immunoglobulin; Off-label indications; Pediatric patients; Malaysia hospital.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100601

A preliminary study of the anti-inflammatory and anti-apoptotic effects of crocin against gastric ischemia-reperfusion injury in rats
Provedor de dados: 60
Autores: Mard,Seyyed Ali; Nikraftar,Zahra; Farbood,Yaghoob; Mansouri,Esrafil.
The aim of the present study was to investigate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats. Thirty-two male rats were randomly divided into sham, I/R, I/R + crocin pretreatment and crocin alone groups. To induce I/R lesions, the celiac artery was clamped for 30 min, and the clamp was then removed to allow reperfusion for 3 h. Crocin-pretreated rats received crocin (15 mg/kg, i.p.) 30 min prior to the induction of I/R injury. Samples of gastric mucosa were collected to quantify the protein expression of caspase-3, an apoptotic factor, and inducible nitric oxide synthase (iNOS), a pro-inflammatory protein, by Western blot. Pretreatment with crocin decreased the total area of gastric lesions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crocin/protective effect; Gastric mucosal/lesions/ ischemia-reperfusion; Gastric lesions/treatment/experimental study; Caspase-3; Inducible nitric oxide synthase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300637

National publications and the BJPS: the future in our hands!
Provedor de dados: 60
Autores: Aranha,Leila R. de Carvalho.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200001

Molecular biology
Provedor de dados: 60
Autores: Hirata,Mario Hiroyuki.
Tipo: Info:eu-repo/semantics/review
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100032

The impacts of Elaeagnus umbellata Thunb. leaf and fruit aqueous extracts on mice hepatic, extrahepatic antioxidant and drug metabolizing enzymes related structures
Provedor de dados: 60
Autores: Ozen,Tevfik; Yildirim,Kemal; Toka,Mehmet.
ABSTRACT In this work, the potential chemopreventive activities of Elaeagnus umbellata fruit aqueous (EUFA) and leaf aqueous (EULA) extracts focusing on the modulatory influence of xenobiotic metabolizing enzymes (XMEs), antioxidant enzymes, glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), lactate dehydrogenase (LDH) activity, lipid peroxidation (LP), sulfhydryl groups were investigated in the hepatic and extrahepatic organs of Swiss albino mice (50 and 100 mg/kg body wt given orally for 14 days) and compared with BHA (0.75 % in diet). The modulatory and chemopreventive properties of two different doses EUFA and EULA were observed for cytochrome P450, cytochrome b5, sulfhydryl groups, NADPH-cytochrome P450 reductase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elaeagnus umbellata Thunb; Chemopreventive properties; Xenobiotic metabolizing enzymes; Antioxidant enzymes; Lipid peroxidation; Glutathione; Lactate dehydrogenase; Glucose-6-phosphate dehydrogenase; 6-Phosphogluconate dehydrogenase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300625

Drug use by elderly inpatients of a philanthropic hospital
Provedor de dados: 60
Autores: Santos,Rômulo Moreira dos; Sette,Ivana Maria Fechine; Belém,Lindomar de Farias.
The high incidence of chronic diseases in the elderly leads to increased intermittent drug therapies. The presence of concomitant diseases and prescriptions made out by various health professionals facilitate the practice of polypharmacy, the emergence of iatrogenic diseases, and therapeutic regimens that are inconvenient for patients. The present study was carried out among elderly patients hospitalized at the Hospital Care Foundation of Paraiba, Campina Grande, with the objectives of studying the consumption of drugs by these patients, noting the possible adverse drug reactions (ADR), drug interactions and the presence of high-risk drugs prescribed to this age group. The study had a descriptive and cross-sectional quantitative design and involved a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly patients/drugs use; Polypharmacy; Drugs/adverse reactions; Drug interactions.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200021

Chemical compositions and antioxidant activity of Heracleum persicum essential oil
Provedor de dados: 60
Autores: Gharachorloo,Maryam; Honarvar,Masoud; Mardani,Shima.
ABSTRACT In this study essential oil of the aerial parts of Heracleum persicum a spice widely used in Iran was isolated by conventional hydrodistillation (HD) and microwave-assisted hydrodistillation (MAHD) techniques. The extraction yield was determined and the chemical compositions of essential oils were identified by the application of gas chromatography/mass spectrometry (GC/MS). The antioxidant activity was determined by two different methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and oven test methods. Although the main compounds of essential oils by the both extraction methods were similar, the essential oil extracted by HD with lower extraction efficiency showed more diverse compounds. The evaluation of antioxidant activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heracleum persicum/essential oil/chemical composition; Heracleum persicum/functional properties; Heracleum persicum/antioxidant activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300623

Development and evaluation of an emulsion containing lycopene for combating acceleration of skin aging
Provedor de dados: 60
Autores: Cefali,Letícia Caramori; Souza-Moreira,Tatiana Maria; Corrêa,Marcos Antônio; Salgado,Hérida Regina Nunes; Isaac,Vera Lucia Borges.
Lycopene, a carotenoid and potent antioxidant is found in large quantities in tomatoes. Lycopene combats diseases, such as cardiovascular disease and different types of cancer, including prostate cancer. However, its topical use in emulsion form for the combat of skin aging is under-explored. The aim of the present study was to develop an emulsion containing lycopene extracted from salad tomatoes and evaluate its cytotoxicity, stability, rheological behavior, antioxidant activity and phytocosmetic permeation. The developed cosmetic comprised an oil phase made up of shea derivatives and was evaluated in terms of its physiochemical stability, spreadability, thermal analysis, rheological behavior, microbiological quality, cytotoxicity, antioxidant activity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carotenoid/antioxidant activity; Lycopene/emulsion form/cytotoxicity; Lycopene/emulsion form/permeation; Lycopene/emulsion form/rheology; Lycopene/emulsion form/thermal analyses; Phytocosmetics; Skin aging/treatment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300579

Myostatin: genetic variants, therapy and gene doping
Provedor de dados: 60
Autores: Yamada,André Katayama; Verlengia,Rozangela; Bueno Junior,Carlos Roberto.
Since its discovery, myostatin (MSTN) has been at the forefront of muscle therapy research because intrinsic mutations or inhibition of this protein, by either pharmacological or genetic means, result in muscle hypertrophy and hyperplasia. In addition to muscle growth, MSTN inhibition potentially disturbs connective tissue, leads to strength modulation, facilitates myoblast transplantation, promotes tissue regeneration, induces adipose tissue thermogenesis and increases muscle oxidative phenotype. It is also known that current advances in gene therapy have an impact on sports because of the illicit use of such methods. However, the adverse effects of these methods, their impact on athletic performance in humans and the means of detecting gene doping are as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myostatin; Myostatin/genetic variants; Myostatin/pharmacological inhibitors; Gene doping; Gene therapy; Physical performance; Skeletal muscle.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300003

Antimicrobial activity assessment of time-dependent release bilayer tablets of amoxicillin trihydrate
Provedor de dados: 60
Autores: Beg,Sarwar; Nayak,Amit Kumar; Kohli,Kanchan; Swain,Suryakanta; Hasnain,MS.
The aim of present study was the assessment of antimicrobial activity of prepared time-dependent release bilayer tablets of amoxicillin trihydrate and in vitro evaluation of drug release by antimicrobial assay using agar plate diffusion method. The bilayer tablets comprised of a delayed and sustained release layer. Direct compression method was used for the preparation of bilayer tablets containing Eudragit-L100 D55 as delayed release polymer, and HPMCK4M and HPMCK15 as sustained release polymers. The prepared bilayer tablets containing amoxicillin trihydrate were evaluated for hardness, thickness, friability, weight variation and drug content. Further, in vitro drug release was assessed by antimicrobial assay using S. aureus and E. coli as test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronotherapeutics; Time-dependent release; In vitro dissolution; Minimum inhibitory concentration; Zone of inhibition.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200010

A fully validated microbiological assay to evaluate the potency of ceftriaxone sodium
Provedor de dados: 60
Autores: Manfio,Maria Luisa; Agarrayua,Danielle Araújo; Machado,Jaison Carlosso; Schmidt,Cleber Alberto.
Ceftriaxone (CFTX) sodium is a third-generation, broad-spectrum cephalosporin that is resistant to beta-lactamases. An alternative bioassay for the assessment of the potency of this drug in pharmaceutical formulations has not been previously reported. Thus, this paper reports the development and full validation of a 3 x 3 agar diffusion bioassay using a cylinder-plate method to quantify CFTX sodium in pharmaceutical samples. The strain Staphylococcus aureus ATCC 6538P was used as the test microorganism, and the results of the proposed bioassay displayed high linearity, precision, accuracy, specificity and robustness. All potency results were statistically analyzed using an analysis of variance (ANOVA) and were found to be linear (r=0.99999) in the range of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ceftriaxone/agar diffusion assay; Ceftriaxone/quality control; Ceftriaxone/microbiological assay; Drugs/quality control.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400015

Erratum: Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity
Provedor de dados: 60
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300901

Intranasal mucoadhesivemicroemulsion for neuroprotective effect of curcuminin mptp induced Parkinson model
Provedor de dados: 60
Autores: Mandal,Snigdha Das; Mandal,Surjyanarayan; Patel,Jayvadan.
ABSTRACT This study was to investigate the neuroprotective effect of curcumin against inflammation-mediated dopaminergic neurodegeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice model of Parkinson's disease (PD). Curcumin loaded sodium hyaluronate based mucoadhesive microemulsion (CMME) was developed by using Box Behnken design of Response surface method (RSM) and was characterized. Male C57BL/6 mice were first treated with four intraperitoneal injections of MPTP (20 mg/kg of body weight) at 2 h intervals followed CMME intranasal administration for 14 days at 2.86 mg of curcumin/kg of body weight per once a day. Optimal CMME containing 3% Capmul MCM as oil phase, 37 % of Accenon CC and Transcutol HP at 2.5:1 ratio and 0.5% sodium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin/neuroprotective effect; Response surface methodology (RSM); Box-Behnken design; Sodium hyaluronate; Neurodegradation; Parkinson's disease/treatment; Male mice C57BL/6.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200621

Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica serotype Typhimurium
Provedor de dados: 60
Autores: Trevisan,Daliah Alves Coelho; Silva,Alex Fiori da; Negri,Melyssa; Abreu Filho,Benício Alves de; Machinski Junior,Miguel; Patussi,Eliana Valéria; Campanerut-Sá,Paula Aline Zanetti; Mikcha,Jane Martha Graton.
ABSTRACT The present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and the time-kill curve and scanning electron microscopy (SEM) were performed to evaluate antibacterial activity. Antibiofilm activity was evaluated by quantifying total biomass using crystal violet assay, and metabolic activity was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The action of carvacrol against preformed biofilm on polypropylene and stainless steel was also evaluated by colony counting and SEM. The MIC and MBC was 312 µg mL-1. Carvacrol at MIC and 2 x MIC eliminated cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carvacrol; Biofilm; Antibacterial activity; Salmonella Typhimurium.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100612

The use of Brazilian vegetable oils in nanoemulsions: an update on preparation and biological applications
Provedor de dados: 60
Autores: Bajerski,Lisiane; Michels,Luana Roberta; Colomé,Letícia Marques; Bender,Eduardo André; Freddo,Rodrigo José; Bruxel,Fernanda; Haas,Sandra Elisa.
ABSTRACT Vegetable oils present important pharmacological properties, which gained ground in the pharmaceutical field. Its encapsulation in nanoemulsions is considered a promising strategy to facilitate the applicability of these natural compounds and to potentiate the actions. These formulations offer several advantages for topical and systemic delivery of cosmetic and pharmaceutical agents including controlled droplet size, protection of the vegetable oil to photo, thermal and volatilization instability and ability to dissolve and stabilize lipophilic drugs. For these reasons, the aim of this review is to report on some characteristics, preparation methods, applications and especially analyze recent research available in the literature concerning the use...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant oils/nanoemulsions/preparation; Plant oils/biological applications.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300347

A review on medicinal plants used for treating ototoxicity and acoustic trauma induced hearing loss
Provedor de dados: 60
Autores: Mahmoudian-sani,Mohammad-Reza; Sheikhshabani,SomayehHashemi; Mirfakhar,Farzaneh Sadat; Asgharzade,Samira.
Hearing loss induced by chemotherapy and acoustic trauma is mainly associated with two factors, free radical formation and apoptosis pathway activation. Despite numerous efforts on reducing the effects of these factors, no definite strategy is still determined to interfere with and control these processes. In recent studies, various protective agents, including antioxidants have been used on animal models, to inhibit the formation of free radicals thus improving hearing loss.In this review article we will discuss the role of traditional herbal medicine in treatment of noise/drug induced hearing loss, focusing on medicinal plants’ active substances,as well as their mechanisms of action in reducing or preventing the formation of free radicals thus increasing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hearing Loss/ prevention &amp;amp; Control Ototoxicity Acoustic Trauma/ physiopathology Herbal Medicine/ instrumentation Oxidative stress/ drug effects Antioxidants/ therapeutic use Hearing Loss Noise-Induced/ therapy Plants Medicinal/ drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100701

Enhancing immune effects of a DNA vaccine against kidney cancer using CD40L as an adjuvant
Provedor de dados: 60
Autores: Jiang,Guohong; Song,Rixin; Ma,Peilong.
The use of specific combinations of antigens and adjuvant represents a promising approach for increasing the immunogenicity of DNA vaccines. In the present study, we evaluated the immunity and antitumor effects of DNA vaccines with G250 as the target antigen in a mouse model of renal cell carcinoma. We constructed two recombinant plasmids, pVAX1-G250 and pVAX1-CD40L. The recombinant plasmids were injected into mice by intramuscular injection and electrical pulse stimulation. ELISA and ELISPOT experiments were performed to evaluate the corresponding humoral and cellular immune responses following immunization. To further investigate the antitumor potential of the DNA vaccines, we established a tumor-bearing mouse model expressing G250 target antigen. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney cancer; G250; CD40L; DNA vaccine.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100555

Evaluation of basil extract ( Ocimum basilicum L.) on oxidative, anti-genotoxic and anti-inflammatory effects in human leukocytes cell cultures exposed to challenging agents
Provedor de dados: 60
Autores: Güez,Camila Martins; Souza,Raul Oliveira de; Fischer,Paula; Leão,Maria Fernanda de Moura; Duarte,Jonathaline Apollo; Boligon,Aline Augusti; Athayde,Margareth Linde; Zuravski,Luísa; Oliveira,Luís Flávio Souza de; Machado,Michel Mansur.
ABSTRACT Ocimum is one of the most important genera of the Lamiaceae family. Several studies about basil and its popular use reveal many characteristics of the herb, including its use as antioxidant, anti-aging, anti-inflammatory, anti-carcinogenic, anti-microbial, and cardiovascular agents, among others. In this paper, we evaluated genotoxic, oxidative, and anti-inflammatory parameters from the extract of Ocimum basilicum in different concentrations, using human leukocytes cultures exposed to challenging agents. Our results confirm that the O. basilicum extract acts as an antioxidant and effectively reverts or subjugates the effects of high oxidizing agents such as hydrogen peroxide. These actions are attributed to its composition, which is rich in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocimum basilicum/evaluation; Ocimum basilicum/genotoxic effects; Ocimum basilicum/anti-inflammatory properties; Medicinal plants/study.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100606

Donepezil: A review of the recent structural modifications and their impact on anti-Alzheimer activity
Provedor de dados: 60
Autores: Mohsin,Noor ul Amin; Ahmad,Matloob.
Alzheimer disease (AD) is characterized by a low level of acetylcholine, beta-amyloid (Aβ) aggregation and oxidative stress. Donepezil is the core medicine used for the treatment of AD. Various structural modifications of donepezil have been carried out. Benzylpiperidine part of donepezil has been replaced with benzylpyridine, pyridyl methylpiperidine, benzylpiperazine, pyrimidyl piperazine. These derived molecules showed promising activities as anti-Alzheimer agents. Replacement of indanone part by other heterocyclic rings such as pyridine resulted in the formation of compounds which exhibited monoamine oxidase (MAO) as well as acetylcholinesterase (AChE) inhibition. Propargylamine containing derivatives displayed AChE as well as MAO inhibition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylcholinesterase (AChE); Butyrylcholinesterase (BuChE); Benzylpiperidine; Indanone; Hybrid compounds.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100702

New perspectives and recommendations for anticoagulant therapy post orthopedic surgery
Provedor de dados: 60
Autores: Kropf,Marcelo; Bergami,Cleidson Alves; Leal,Felipe Dias; Passos,Claudia Oliveira Dias; Gonsalves,Zilda de Santana; Marques,Isabela Laudares; Mota,Isabela Azevedo; Gonçalves,Marcele Lima Monte.
Anticoagulant therapy is essential for the prevention of risks associated with the formation of thrombus in patients after surgery, especially in orthopedics. Recently, new oral anticoagulants were introduced in the therapeutic arsenal. This fact is important, because the current drug of choice in clinical practice is enoxaparin, a low molecular weight heparin. As all injecting drugs, enoxaparin may reduce patients' adherence to treatment by dissatisfaction with and resistance to the administration. This article reviews the available literature on the overall utility of these innovative medicines, approaching the pharmacology, the compared efficacy in relation to current agents, and the potential targets for new agents, as well as points to new trends in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral anticoagulants/use; Oral anticoagulants/patient compliance; Injectable anticoagulants/use; Injectable anticoagulants/patient compliance; Anticoagulant therapy; Enoxaparin/use; Dabigatrana/use; Rivaroxabana/use; HPBM drugs/use.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400002

Ferulic acid and derivatives: molecules with potential application in the pharmaceutical field
Provedor de dados: 60
Autores: Paiva,Lívia Brenelli de; Goldbeck,Rosana; Santos,Wanderley Dantas dos; Squina,Fabio Marcio.
Ferulic acid is a phenolic acid widely distributed in the plant kingdom. It presents a wide range of potential therapeutic effects useful in the treatments of cancer, diabetes, lung and cardiovascular diseases, as well as hepatic, neuro and photoprotective effects and antimicrobial and anti-inflammatory activities. Overall, the pharmaceutical potential of ferulic acid can be attributed to its ability to scavenge free radicals. However, recent studies have revealed that ferulic acid presents pharmacological properties beyond those related to its antioxidant activity, such as the ability to competitively inhibit HMG-CoA reductase and activate glucokinase, contributing to reduce hypercholesterolemia and hyperglycemia, respectively. The present review...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ferulic acid/properties; Ferulic acid/antioxidant activity; Ferulic acid/dietary sources; Ferulic acid/therapeutic effects; Natural products; Pharmacognosy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300002

Oral lactate intensifies insulin toxicity during severe insulin-induced hypoglycemia in mice
Provedor de dados: 60
Autores: Vilela,Vanessa Rodrigues; Antunes,Marina Masetto; Godoi,Vilma Aparecida Ferreira; Travassos,Patricia Batista; Souza,Helenir Medri de; Bazotte,Roberto Barbosa.
ABSTRACT We investigated whether oral lactate could prevent seizures and deaths in mice with severe hypoglycemia induced by a high dose of insulin. For this purpose, mice were fasted for 15 h and then given an intraperitoneal injection of regular insulin (5.0 U/kg or 10.0 U/kg). Immediately after insulin injection, the mice received an oral dose of saline (control), glucose (5.5 mmol/kg), or lactate (18.0 mmol/kg). Glucose and lactate levels were measured in the blood and brain before and after the seizures began. Glucose and lactate delayed (p &lt; 0.05) the onset of seizures associated with severe insulin-induced hypoglycemia. Elevated (p &lt; 0.05) brain levels of lactate were associated with an absence of seizures in mice that received glucose or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactatemia; Oral glucose; Diazepam; Seizure; Anticonvulsant effect.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200602

Residues of Citrus sinensis (L.) Osbeck as agents that cause a change in antioxidant defense in plants
Provedor de dados: 60
Autores: Nunes,Patrícia Mayra Pavan; Silva,Cristiane Bezerra da; Paula,Cristiane da Silva; Smolarek,Fernanda Ferreira; Zeviani,Walmes Marques; Chaves,Suelen Cristina; Lorini,Fernando; Dias,Josiane de Fátima Gaspari; Miguel,Obdulio Gomes; Zanin,Sandra Maria Warumby; Miguel,Marilis Dallarmi.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: &lt; Italic&gt; Citrus sinensis&lt; /italic&gt; (L.) Osbeck/residues/utilization &lt; Italic&gt; Citrus sinensis&lt; /italic&gt; (L.) Osbeck/seeds/allelopathic potential Rutaceae/residues/utilization Fixed oil Fatty acid.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200479

Cholesterol improves the transfection efficiency of polyallylamine as a non-viral gene delivery vector
Provedor de dados: 60
Autores: Oskuee,Reza Kazemi; Ramezanpour,Mahdieh; Gholami,Leila; Malaekeh-Nikouei,Bizhan.
ABSTRACT Cationic polymers such as polyallylamine (PAA) having primary amino groups are poor transfection agents and possess a high cytotoxicity index when used without any chemical modification. In this study, PAA was modified with cholesterol in order to improve transfection efficiency and to reduce cytotoxicity. PAA polymers with molecular weights of 15 and 65 kDa were selected and grafted with cholesterol at percentages of 5, 10, 15, 30, and 50. After purification, the efficacy of the synthetic vectors was evaluated in terms of DNA condensation using the ethidium bromide test, buffering capacity, particle size, zeta potential, transfection efficiency, and cytotoxicity assay in Neuro2A cell lines. According to the ethidium bromide test, these vectors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterol/ efficiency; Gene delivery; Non-viral vectors/evaluation; Polyallylamine Transfection efficiency; Cytotoxicity/study.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300612

D-α-tocopherol reduces renal damage in hypertensive rats
Provedor de dados: 60
Autores: Pletiskaitz,Thaís Maria da Fonseca; Gomes,Guiomar Nascimento.
This study investigated the beneficial effects of D-α-tocopherol supplementation in protecting against the renal morphological and functional changes caused by hypertension. Spontaneously hypertensive (SHR) and normotensive control (WKY) rats received D-α-tocopherol (80 mg/kg by gavage) or vehicle (mineral oil) every other day for 60 days, from the age of 2 months. After this treatment period, all animals were assessed for renal morphological and functional parameters. The glomerular hypertrophy, increased interlobular wall thickness and enlarged renal vascular resistance found in SHR were reduced by D-α-tocopherol treatment. Sodium and volume retention observed in SHR were also decreased by D-α-tocopherol treatment. Moreover, D-α-tocopherol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Renal injury; Glomerular enlargement; Vascular hypertrophy; D-α-tocopherol.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200013

Composition, antioxidant properties, and biological activities of the essential oil extracted from Ocotea diospyrifolia (Meisn.) Mez.
Provedor de dados: 60
Autores: Fabri,Natasha Tiemi; Gatto,Larissa Junqueira; Furusho,Alethéia Santos; Garcia,Meira Janette Ballestros; Marques,Francisco de Assis; Miguel,Marilis Dallarmi; Montrucchio,Deise Prehs; Zanin,Sandra Maria Warumby; Miguel,Obdulio Gomes; Dias,Josiane de Fátima Gaspari.
The article has aimed to characterize the essential oil extracted from Ocotea diospyrifolia (Meisn.) Mez. leaves, in terms of its chemical composition and antioxidant, hemolytic, and phytotoxic potentials, as well as its toxicity against Artemia salina. The major constituents identified by CG-MS were d-elemene, spathulenol, and b-atlantol. When screened for potential biological activities, the essential oil presented low toxicity against Artemia salina, and a capacity of lysing red blood cells. Also, the evaluation of its in vitro antioxidant activity, using the phosphomolybdenum method, showed better results when compared to butylhydroxytoluene (BHT) and rutin. In conclusion, the results obtained showed certain in vitro toxicity, leading to an interesting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocotea diospyrifolia; Lauraceae; Spathulenol; Antioxidant; Toxicity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100593

Differential pulse voltammetric determination of diclofenac in pharmaceutical preparations and human serum
Provedor de dados: 60
Autores: Yilmaz,Bilal; Kaban,Selcuk; Akcay,Bilge Kagan; Ciltas,Ulvihan.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diclofenac/determination; Differential pulse voltammetry/quantitative analysis; Pharmaceutical formulations/analysis; Human serum/analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200285

Development and physical evaluation of Maytenus ilicifolia effervescent granules using factorial design
Provedor de dados: 60
Autores: Cunha-Filho,Marcilio Sérgio Soares da; Gustmann,Pricila Castilho; Garcia,Felipe Sanches; Lima,Eliana Martins; Sá-Barreto,Lívia Cristina Lira de.
The medicinal plant Maytenus ilicifolia is a commonly used phytomedicine for the treatment of gastritis. The high dose required and low density of these extracts make necessary a daily intake of several capsules, hindering adherence to the medication. The purpose of this work was to develop a suitable dosage form for the administration of Maytenus ilicifolia using effervescent granules. A 23 factorial design was used to study the physical characteristics of the granules (particle size distribution, repose angle, Carr index, scanning electron microscopy and disintegration time). Moisture stability was also determined. According to the experimental design, granule size is the most important factor in determining the flow characteristics of effervescent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maytenus ilicifolia/pharmacognosy; Maytenus ilicifolia/Effervescent granules; Effervescent granules/physical characteristics/factorial design; Effervescent granules/water sorption behavior; Plant extracts/administration vehicle.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200243

Comparative analyses of response surface methodology and artificial neural networks on incorporating tetracaine into liposomes
Provedor de dados: 60
Autores: Pereira,Ana Karina Vidal; Barbosa,Raquel de Melo; Fernandes,Marcelo Augusto Costa; Finkler,Leandro; Finkler,Christine Lamenha Luna.
This study evaluated the incorporation of tetracaine into liposomes by RSM (Response Surface Methodology) and ANN (Artificial Neural Networks) based models. RCCD (rotational central composite design) and ANN were performed to optimize the sonication conditions of particles containing 100 % lipid. Laser light scattering was used to perform measure hydrodynamic radius and size distribution of vesicles. The liposomal formulations were analyzed by incorporating the drug into the hydrophilic phase or the lipophilic phase. RCCD and ANN were conducted, having the lipid/cholesterol ratio and concentration of tetracaine as variables investigated and, the encapsulation efficiency and mean diameter of the vesicles as response variables. The optimum sonication...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Local anesthetics; Liposomes; Response Surface Methodology; Artificial Neural Networks.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100517

Quick and simple LC-MS/MS method for the determination of simvastatin in human plasma: application to pharmacokinetics and bioequivalence studies
Provedor de dados: 60
Autores: Silva,Suéllen Cristina Rennó; Rezende,Gustavo Rodrigues de; Boralli,Vanessa Bergamin.
A simple, rapid, and sensitive method based on liquid chromatography-tandem mass spectrometry for the quantitative determination of simvastatin in human plasma was developed and validated. After a simple extraction with methyl tert-butyl ether, the analyte and internal standard (lovastatin) were analyzed using reverse-phase liquid chromatography, on a Kinetex C18column (100 × 4.6 mm, 2.6 μm) using acetonitrile: ammonium acetate (2 mM + 0.025 % formic acid) (70: 30, v/v) as a mobile phase in a run time of 3.5 min. Detection was carried out using electrospray positive ionization mass spectrometry in the multiple-reaction monitoring mode. The method was linear over 0.04-40.0 ng/mL concentration range. The mean extraction recovery of simvastatin was 82% (RSD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simvastatin/determination; Liquid chromatography/quantitative analysis; Tandem mass spectrometry/quantitative analysis; Human plasma/determination of simvastatin; Simvastatin/bioequivalence; Simvastatin/pharmacokinetics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300543

Environmental contamination by fluoroquinolones
Provedor de dados: 60
Autores: Frade,Verônica Maria Fadário; Dias,Meriellen; Teixeira,Antonio Carlos Silva Costa; Palma,Mauri Sergio Alves.
Over the past few decades, a high number of pharmaceuticals have been detected in surface, ground and drinking waters. This contamination comes from domestic sewage, livestock, hospitals and chemical-pharmaceutical industries. Typical examples of these pollutants are the fluoroquinolones - powerful antibiotics used in human and veterinary medicine. The presence of fluoroquinolones in the environment can pose a serious threat to the ecosystem and to human health due to their high consumption globally: in 1998, around 120 tons were produced. Even at low environmental concentrations, antibiotics stimulate bacterial resistance. The consequences of the presence of fluoroquinolones in the environment are not fully understood, but are known to be toxic to plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoroquinolones/waste/environmental impact; Fluoroquinolones/waste/environmental contamination; Environmental contamination; Advanced oxidation processes/wastewater treatment.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100004

Development of a multiparticulate system containing enteric-release mini-tablets of omeprazole
Provedor de dados: 60
Autores: Tondo Filho,Volnei Jose; Andreazza,Itamar Francisco; Sato,Mayumi Elisa Otsuka; Murakami,Fábio Seigi.
The main aim of this study was to develop a multiparticulate system containing mini-tablets of omeprazole formulated with an enteric polymer with pH-dependent solubility. Pre-formulation studies showed good flow and compaction capacity, leading to the production ofhigh quality mini-tablets. The mini-tablets were coated in a fluidized bed with hydroxypropylmethylcellulose /Eudragit(r) L30D55 and packed into hard gelatin capsules. The dissolution profile showed gastro-resistance and zero-order kinetics. The dissolution profile for the formulation containing lactose as the diluent and coated with 12% (tablet weight gain) of polymer was similar to that ofthe reference drug.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiparticulate system/modified release; Enteric release mini-tablets/development; Enteric release mini-tablets/evaluation; Omeprazole/dissolution.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300505

Open access, authors' right, and scientific academy duty: an undelayable commitment!
Provedor de dados: 60
Autores: Aranha,Leila R. de Carvalho.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200001

Medication errors: classification of seriousness, type, and of medications involved in the reports from a university teaching hospital
Provedor de dados: 60
Autores: Dalmolin,Gabriella Rejane dos Santos; Rotta,Eloni Terezinha; Goldim,José Roberto.
Medication errors can be frequent in hospitals; these errors are multidisciplinary and occur at various stages of the drug therapy. The present study evaluated the seriousness, the type and the drugs involved in medication errors reported at the Hospital de Clínicas de Porto Alegre. We analyzed written error reports for 2010-2011. The sample consisted of 165 reports. The errors identified were classified according to seriousness, type and pharmacological class. 114 reports were categorized as actual errors (medication errors) and 51 reports were categorized as potential errors. There were more medication error reports in 2011 compared to 2010, but there was no significant change in the seriousness of the reports. The most common type of error was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medication/errors; Drug therapy; Patients/safety.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400019

Modulation of the oxidative stress in malaria infection by clotrimazole
Provedor de dados: 60
Autores: Rodrigues Henriques,Juan Ricardo; Gamboa de Domínguez,Neira.
Antimycotic clotrimazole (CTZ) has demonstrated remarkable activity against Plasmodium falciparum in vitro and in vivo. Hemoglobin degradation by Plasmodium parasites makes amino acids available for protein synthesis, inducing oxidative stress in infected cells and producing free heme. These events represent biochemical targets for potential antimalarials. In this study, we have tested the ability of CTZ to modify the oxidative status in Plasmodium berghei-infected erythrocytes. After hemolysis, activities of superoxide dismutase (SOD), catalase (CAT), glutathione cycle and NADPH+H+-producing dehydrogenases were investigated using UV-visible spectrophotometry. Thiobarbituric acid reactive substances (TBARS) were evaluated as a marker of lipid damage....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-Hematin; Clotrimazole/modulatory ability; Clotrimazole/oxidative stress; Oxidative stress; Plasmodium berghei; Eritrócitos infectados.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300019

Ability of two new thiazolidinediones to downregulate proinflammatory cytokines in peripheral blood mononuclear cells from children with asthma
Provedor de dados: 60
Autores: Rêgo,Moacyr Jesus Barreto de Melo; Azoubel-Antunes,Adriana; Bezerra,Mariana Brayner-Cavalcanti Freire; Pereira,Michelly Cristiny; Silva,Juliana Cruz da; Lins,Thiago Ubiratan Lins e; Sarinho,Emanuel Sávio Cavalcanti; Amorim,Cézar Augusto da Cruz; Lima,Maria do Carmo Alves de; Galdino-Pitta,Marina Rocha; Pitta,Ivan da Rocha; Pitta,Maira Galdino da Rocha.
Allergic asthma is a chronic, complex inflammatory disease of the airway. Despite extensive studies on the immunomodulation of T helper (Th) cell pathways (i.e., Th1 and Th2) in asthma, little is known about the effects of Th17 pathway modulation, particularly that involving peroxisome proliferator-activated receptors (PPARs). In response, two new thiazolidinedione derivatives-namely, LPSF-GQ-147 and LPSF-CR-35 were synthesized and evaluated for their immunomodulatory effects on Th17-related cytokines, including interferon γ (IFNγ), interleukin IL-6, IL-17, and IL-22 in the peripheral blood mononuclear cells of asthmatic children. Both compounds were nontoxic even at high concentrations (i.e., 100 µM). The LPSF-CR-35 compound significantly reduced the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Th17-related cytokines; Thiazolidinedione derivative.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300602

Adherence to medication before and after the use of a Drug-Dispensing System with usage control
Provedor de dados: 60
Autores: Vieira,Liliana Batista; Ueta,Julieta; Pereira,Leonardo Régis Leira.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medication/treatment adherence; Medicines/safety use; Medicines/dispensing; Drug-Dispensing System and Use Control; Polymedication.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200329

v.48, n.3, 2012
Provedor de dados: 60
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400027

Metabolic dysregulation in early onset psychiatric disorder before and after exposure to antipsychotic drugs
Provedor de dados: 60
Autores: Shah,Rehmat; Subhan,Fazal; Sultan,Syed Muhammad; Haq,Matiul; Ahmad,Hussain; Khan,Qasim Riaz; Ali,Gowhar; Ullah,Sami; Ullah,Ihsan.
Antipsychotic Drugs (APDs) are being widely prescribed to treat various disorders, including schizophrenia and bipolar disorder; however, abnormal glucose metabolism and weight gain have been reported with Atypical Anti-Psychotic drugs (AAPDs) that can lead to insulin-resistance and type 2 diabetes mellitus. The study was designed to assess various biochemical parameters including insulin and blood sugar before and after exposure to APDs in order to exclude the involvement of psychiatric disorders and certain other factors in metabolic dysregulations. Fifty seven APDs-naïve patients with first episode psychosis were divided into six groups who received olanzapine, quetiapine, risperidone, aripiprazole, haloperidol or combination of olanzapine with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olanzapine; Quetiapine; Risperidone; Antipsychotics; Pancreas; Insulin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100535

Variables associated with the post-treatment healing of lesions in patients with American cutaneous leishmaniasis in Paraná State, Brazil
Provedor de dados: 60
Autores: Casavechia,Maria Teresinha Gomes; Silveira,Thaís Gomes Verzignassi; Teodoro,Ueslei; Janeiro,Vanderly; Udo,Margareth; Campana Lonardoni,Maria Valdrinez.
The purpose of this study was to investigate the relationship of several variables to the healing of lesions in patients with American cutaneous leishmaniasis (ACL). The patients with clinical and/or laboratorial diagnoses of the disease were followed up for varying periods after treatment by clinical evaluation and indirect immunofluorescence assay (IFA), from September 2000 to December 2003. The lesions of 85.3% of the 163 patients had healed by their last return for clinical evaluation, and of these, 82.7% had negative IFA results, indicating an association between the healing of lesions and IFA negativity (p=0.000). In patients evaluated up to 120 days after treatment, there was a significant association between negative IFA results and the healing of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis/treatment; Leishmania (Viannia) braziliensis; Cutaneous lesion/healing.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400030

Assessment of pharmacotherapeutic safety of medical prescriptions for elderly residents in a long-term care facility
Provedor de dados: 60
Autores: Varallo,Fabiana Rossi; Ambiel,Ingrid Stephanie Stein; Nanci,Luana Orlandelli; Galduróz,José Carlos Fernandes; Mastroianni,Patricia de Carvalho.
The present study aimed to estimate the prevalence of elderly using potentially inappropriate medications (PIM) and with occurrence of potentially hazardous drug interactions (PHDI); to identify the risk factors for the prescription of PIM and to evaluate the impact of pharmaceutical intervention (PI) for the prescription of safer therapeutic alternatives. Therefore, a cross-sectional study was performed in a long-term care facility in São Paulo State, between December/2010 and January/2011. The medical records of the patients &gt;60 years old who took any drugs were consulted to assess the pharmacotherapeutic safety of the medical prescriptions, in order to identify PIM and PHDI, according to the Beers (2003) and World Health Organization criteria,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/interactions; Elderly people/inappropriated use of medicines; Long-term care facility; Medication/errors; Safety management.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300015

Protective effects of Urtica dioica L. seed extract on liver tissue injury and antioxidant capacity in irradiated rats
Provedor de dados: 60
Autores: Yıldızhan,Kenan; Demirtaş,Ömer Can; Uyar,Ahmet; Huyut,Zübeyir; Çakir,Tahir; Keleş,Ömer Faruk; Yener,Zabit.
Radiotherapy is often used for the treatment of cancer. However, it causes some side effects in patients. This study aimed to determine the hepatoprotective effects of Urtica dioica L. seed-extract (UDSE) in radiation-induced liver injury. Thirty-two male rats were randomly divided into 4 groups (n=8): control(C) group: no action was taken; radiation (R) group: irradiation was administrated at 5Gy single-fraction, radiation with UDSE(R+UDSE) group: irradiation was administrated at 5 Gy single-fraction and animals were fed pellets with 30 mL UDSE/kg; UDSE group: animals were fed pellets with 30 mL UDSE/kg. All of the experiments were performed in all of the groups over 10 days. Malondialdehyde (MDA) and reduced-glutathione (GSH) levels and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiation; Urtica dioica L; Seed extract; Oxidative stress; Antioxidant; Tissue injury.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100536

RP-HPLC method development and validation for estimation of rivaroxaban in pharmaceutical dosage forms
Provedor de dados: 60
Autores: Çelebier,Mustafa; Reçber,Tuba; Koçak,Engin; Altinöz,Sacide.
Rivaroxaban, an anti-clotting medication, acts at a crucial point in the blood-clotting process and stops the formation of blood clots. In this study, RP-HPLC method was developed for the determination of rivaroxaban in tablets (Xarelto® (10 mg)). Phenomenex Luna 5 µm C18 100 Å LC Column (250 x 4.6 mm) was used at 40 ºC. Isocratic elution was performed with ACN:Water (55:45 v/v) mixture. The flow rate was 1.2 mL min-1 and UV detection was at 249 nm. Internal standard (Caffeine) and rivaroxaban were eluted within 2.21 and 3.37 minutes, respectively. The developed method was validated according to the ICH guidelines and found to be linear within the range 0.005 - 40.0 µg mL-1. The method was accurate, precise, robust and rapid. Thus, it was applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPLC; Rivaroxaban; Validation; System suitability; Stability-indicating; Pharmaceutical dosage form.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200018

Biodegradable microparticulate drug delivery system of diltiazem HCl
Provedor de dados: 60
Autores: Gupta,Manish Kumar; Prakash,Deepak; Mishra,Brahmeshwar.
The efficacy of a drug in a specific application requires the maintenance of appropriate drug blood level concentration during a prolonged period of time. Controlled release delivery is available for many routes of administration and offers many advantages (as microparticles and nanoparticles) over immediate release delivery. These advantages include reduced dosing frequency, better therapeutic control, fewer side effects, and, consequently, these dosage forms are well accepted by patients. Advances in polymer material science, particle engineering design, manufacture, and nanotechnology have led the way to the introduction of several marketed controlled release products and several more are in pre-clinical and clinical development. The objective of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diltiazem HCl loaded microparticles/preparation; Diltiazem HCl loaded microparticles/release profile; Diltiazem HCl loaded microparticles/evaluation; Albumin/microparticles; Microparticles batches/diffusion kinetics; Cloridrato de diltiazem/associado a micropartículas; Cloridrato de diltiazem/avaliação do perfil de liberação.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400014

pH-sensitive polyvinylpyrrolidone-acrylic acid hydrogels: Impact of material parameters on swelling and drug release
Provedor de dados: 60
Autores: Sohail,Kashif; Khan,Ikram Ullah; Shahzad,Yasser; Hussain,Talib; Ranjha,Nazar Muhammad.
In this study, we fabricated pH-sensitive polyvinylpyrrolidone/acrylic acid (PVP/AA) hydrogels by a free-radical polymerisation method with variation in the content of monomer, polymer and cross-linking agent. Swelling was performed in USP phosphate buffer solutions of pH 1.2, 5.5, 6.5 and 7.5 with constant ionic strength. Network structure was evaluated by different parameters and FTIR confirmed the formation of cross-linked hydrogels. X-ray crystallography showed molecular dispersion of tramadol HCl. A drug release study was carried out in phosphate buffer solutions of pH 1.2, 5.5 and 7.5 for selected samples. It was observed that swelling and drug release from hydrogels can be modified by changing composition and degree of cross-linking of the hydrogels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/release; Hydrogels/pH sensitive; Polyvinylpyrrolidone-acrilic acid/hidrogels; Tramadol hydrochloride; Methylene bisacrylamide.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100018

HPLC method for the simultaneous determination of four compounds in rat plasma after intravenous administration of Portulaca oleracea L. extract
Provedor de dados: 60
Autores: Cheng,Zhongzhe; Xie,Ming; Zhang,Wenjie; Cheng,Lan; Du,Yang; Wang,Yunjiao; Ying,Xixiang; Kang,Tingguo.
The objective of the present study was to develop a simple and selective HPLC method for the simultaneous determination of hesperidin (HP), caffeic acid (CA), ferulic acid (FA) and p-coumaric acid (p-CA) in rat plasma after intravenous administration of Portulaca oleracea L. extract (POE). With the hyperoside as the internal standard, the sample pretreatment procedure involved simple single-step extraction with methanol of 0.2 mL plasma. The mobile phase consisted of methanol-acetonitrile-tetrahydrofuran-0.5% glacial acetic acid (5:3:18:74, v/v/v/v). The calibration curves were linear over the range of 0.1-25 µg mL-1, 0.1-25 µg mL-1, 0.1-25 µg mL-1and 0.015-3 µg mL-1 for HP, CA, FA and p-CA, respectively. The method developed was suitable for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Portulaca oleracea L./pharmacognosy; High Performance Liquid Chromatography/rat plasma; Vegetal extract/pharmacokinetic study; Medicinal plants.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100018

Formulation and evaluation of topical herbal gel for the treatment of arthritis in animal model
Provedor de dados: 60
Autores: Aiyalu,Rajasekaran; Govindarjan,Arulkumaran; Ramasamy,Arivukkarasu.
ABSTRACT The objective of the study is to formulate and evaluate a topical herbal gel containing Cardiospermum halicacabum and Vitex negundo leaf extracts for their anti-arthritic activity in rats. Twelve herbal gel formulations were prepared using 1.5% of gelling agents carbopol 934 (F1-F6) and carbopol 940 (F6-F12) and they were evaluated for physical appearance, net content, viscosity, extrudability, pH, spreadability, in vitro diffusion profile and primary skin irritation tests. The stability study for the topical herbal gel formulation was done as per ICH guidelines and anti-arthritic activity was evaluated by Freund's Complete Adjuvant (FCA) induced arthritis method. Assessment of body weight, paw volume, hematological and biochemical parameters,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthritis/treatment/herbal gel; Cardiospermum halicacabum/topical herbal gel; Vitex negundo/topical herbal gel; Medicinal plants/arthritis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300493

Optimization of cosmetic formulations development using Box-Behnken design with response surface methodology: physical, sensory and moisturizing properties
Provedor de dados: 60
Autores: Mercurio,Daiane Garcia; Calixto,Lívia Salomão; Campos,Patrícia Maria Berardo Gonçalves Maia.
Considering the importance of an adequate composition of the formulation in the development of stable, safe and effective cosmetic products, experimental design techniques are tools that can optimize the formulation development process. The objective of this study was to develop topical formulations using the Box-Behnken design with response surface methodology and evaluate its physical, sensory and moisturizing properties. The experimental design used in the first step allowed to identify and to quantify the influence of raw materials, as well as the interaction between them. In the second step, the analysis identified the influence of soy lecithin, the phytantriol and capric acid triglyceride and caprylic on the consistency index, stickiness and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emulsions; Formulation; Stability; Skin barrier; Experimental design.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100624

Dissolution test of herbal medicines containing Paullinia cupana: validation of methods for quantification and assessment of dissolution
Provedor de dados: 60
Autores: Sousa,Sandra Alves de; Pascoa,Henrique; Conceição,Edemilson Cardoso da; Alves,Suzana Ferreira; Diniz,Danielle Guimarães Almeida; Paula,José Realino de; Bara,Maria Teresa Freitas.
"Guaraná" (Paullinia cupana) is used as a physical activity enhancer and stimulator due to its methylxanthines and condensed tannins. The aim of this work was to evaluate the dissolution behavior of five herbal medicines in the form of capsules and tablets containing guaraná. Assay and dissolution methods were validated and results obtained allowed simultaneous marker quantification with precision, accuracy, selectivity and robustness. Findings showed that 100% of the herbal medicinal products analyzed did not provide satisfactory results concerning the presence of four markers, 60% had three markers (caffeine, catechin and epicatechin), while 40% had only caffeine at tested dosage forms. In addition, after 30 minutes, only capsule A showed at least 80% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbal medicines/quality control; Herbal medicines/dissolution behavior; Medicinal plants; Guaraná/pharmacognosy; Paullinia cupana/pharmacognosy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200008

Visão retrospectiva em fibras alimentares com ênfase em beta-glucanas no tratamento do diabetes
Provedor de dados: 60
Autores: Mira,Giane Sprada; Graf,Hans; Cândido,Lys Mary Bileski.
Fibra alimentar refere-se aos componentes de plantas ou carboidratos análogos que são resistentes à digestão e absorção no intestino delgado humano. A American Dietetic Association recomenda ingestão de 20-35g de fibras ao dia. Evidências sugerem que uma dieta rica em fibras traz benefícios à manutenção da saúde, redução de risco e tratamento de doenças crônicas como a obesidade, doenças cardiovasculares, diverticulite e diabetes. As fibras são classificadas de acordo com a sua solubilidade em solúveis e insolúveis, com efeitos fisiológicos distintos. As insolúveis são responsáveis pelo aumento do bolo fecal e diminuição do tempo de trânsito intestinal. As solúveis retardam o esvaziamento gástrico e a absorção de glicose diminuindo a glicemia pós-prandial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibras alimentares; Beta-glucanas; Diabetes/tratamento; Glicemia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100003

Nanoemulsions as vehicles for transdermal delivery of glycyrrhizin
Provedor de dados: 60
Autores: Harwansh,Ranjit Kumar; Patra,Kartik Chandra; Pareta,Surendra Kumar; Singh,Jagadish; Rahman,Mohammed Akhlaquer.
The present investigation aims to evaluate an isotropic and thermodynamically stable nanoemulsion formulation for transdermal delivery of glycyrrhizin (GZ), with minimum surfactant and cosurfactant (Smix) concentrations that could improve its solubility, permeation enhancement, and stability. Pseudo-ternary phase diagrams were developed and various nanoemulsion formulations were prepared using soyabean oil as oil, Span 80, Brij 35 as a surfactant and isopropyl alcohol as a cosurfactant. Nanoemulsion formulations that passed the thermodynamic stability tests were characterized for pH, viscosity and droplet size using a transmission electron microscopy. The transdermal ability of glycyrrhizin through human cadaver skin was determined using Franz diffusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoemulsions/use; Nanoemulsions/in vitro skin permeation; Glycyrrhizin/transdermal delivery; Isotropic nanoemulsion formulation/evaluation; Anti-inflammatories/transdermal delivery; Surfactants; Permeation/enhancers; Permeation/in vitro study.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400014

Management of effluents and waste from pharmaceutical industry in Minas Gerais, Brazil
Provedor de dados: 60
Autores: Deschamps,Eleonora; Vasconcelos,Olivia; Lange,Lisete; Donnici,Claudio Luis; Silva,Merces Coelho da; Sales,Juliana Aparecida.
Today the management of solid waste and wastewater is a major concern for humanity. In the last decade, traces of pharmaceuticals have been reported in the water cycle and have raised concerns among regulators, water suppliers and the public regarding the potential risks to human health. This study evaluated solid waste management in the state of Minas Gerais and concluded that the main fate of hazardous waste has been incineration, while the non-hazardous waste has been recycled or sent to landfills. However, complaints to the Environmental Agency - FEAM have indicated that a significant number of companies just send their hazardous wastes to landfills or even to garbage dumps, thus highlighting the urgent need for adequate waste management in Minas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical industry/management of waste; Solid waste/management; Solid waste/pharmaceutical industry; Waste water/management; Waste water/pharmaceutical industry; Brazil/pharmaceutical industry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400017

Design and characterization of nanocarriers loaded with Levofloxacin for enhanced antimicrobial activity; physicochemical properties, in vitro release and oral acute toxicity
Provedor de dados: 60
Autores: Siafaka,Panoraia; Okur,Mehmet Evren; Ayla,Şule; Er,Sevda; Cağlar,Emre Şefik; Okur,Neslihan Üstündağ.
Inorganic and carbon based nanomaterials are widely used against several diseases, such as cancer, autoimmune diseases as well as fungi and bacteria colonization. In this work, Santa Barbara Amorphous mesoporous silica (SBA), Halloysite Nanotubes (HNTs) and Multiwalled Carbon Nanotubes (CNTs) were loaded with fluoroquinolone Levofloxacin (LVF) to be applied as antimicrobial agents. The prepared via adsorption nanocarriers were characterized by Fourier-Transformed Spectroscopy, Scanning Electron Microscopy as well as High Pressure liquid Chromatography. In vitro release studies were carried out using Simulated Body Fluid at 37oC and data analyzed by various kinetic models showing slow dissolution over 12-24 hours. Antimicrobial studies showed improved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbon nanotubes; Santa barbara amorphous; Halloysite nanotubes; Levofloxacin; Toxicity; Antimicrobial.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100514

Simultaneous RP-HPLC determination of sparfloxacin and dexamethasone in pharmaceutical formulations
Provedor de dados: 60
Autores: Razzaq,Syed Naeem; Ashfaq,Muhammad; Mariam,Irfana; Khan,Islam Ullah; Razzaq,Syed Saleem.
The present study describes the development and subsequent validation of simple and accurate stability indicating RP-HPLC method for the determination of sparfloxacin and dexamethasone in pharmaceutical formulations in the presence of their stress-induced degradation products. Both the drugs and their stress-induced degradation products were separated within 10 minutes using C8 column and mixture of methanol and 0.02 M phosphate buffer pH 3.0 (60:40 v/v, respectively) as mobile phase at 270 nm using diode array detector. Regression analysis showed linearity in the range of 15-105 µg/mL for sparfloxacin and 5-35 µg/mL for dexamethasone. All the analytes were adequately resolved with acceptable tailing. Peak purity of the two drugs was also greater than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RP-HPLC; Sparfloxacin; Dexamethasone; Degradation products; Stability studies.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200012

Extended release promethazine HCl using acrylic polymers by freeze-drying and spray-drying techniques: formulation considerations
Provedor de dados: 60
Autores: Tiwari,Ruchi; Srivastava,Birendra; Tiwari,Gaurav; Rai,Awanik.
The present study investigated a novel extended release system of promethazine hydrochloride (PHC) with acrylic polymers Eudragit RL100 and Eudragit S100 in different weight ratios (1:1 and 1: 5), and in combination (0.5+1.5), using freeze-drying and spray-drying techniques. Solid dispersions were characterized by Fourier-transformed infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), Powder X-ray diffractometry (PXRD), Nuclear magnetic resonance (NMR), Scanning electron microscopy (SEM), as well as solubility and in vitro dissolution studies in 0.1 N HCl (pH 1.2), double-distilled water and phosphate buffer (pH 7.4). Adsorption tests from drug solution to solid polymers were also performed. A selected solid dispersion system was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Promethazine hydrochloride/extended release; Drugs/extended release; Eudragit RL100; Eudragit S100; Acrylic polymers; Spray drying/pharmacotechnics; Freeze drying/pharmacotechnics; Extended realease/pharmacotechnics; Pharmaceutical formulations/evaluation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400029

Drug discovery and computational strategies in the multitarget drugs era
Provedor de dados: 60
Autores: Viana,Jéssika de Oliveira; Félix,Mayara Barbalho; Maia,Mayara dos Santos; Serafim,Vanessa de Lima; Scotti,Luciana; Scotti,Marcus Tullius.
The pharmaceutical industry is increasingly joining chemoinformatics in the search for the development of new drugs to be used in the treatment of diseases. These computational studies have the advantage of being less expensive and optimize the study time, and thus the interest in this area is increasing. Among the techniques used is the development of multitarget directed ligands (MTDLs), which has become an ascending technique, mainly due to the improvement in the quality of treatment involving several drugs. Multitarget therapy is more effective than traditional drug therapy that emphasizes maximum selectivity for a single target. In this review a multitarget drug survey was carried out as a promising strategy in several important diseases: neglected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug design; Multitarget compounds; In silico; Diseases.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700409

The effect of physical exercise and caloric restriction on the components of metabolic syndrome
Provedor de dados: 60
Autores: Torres-Leal,Francisco Leonardo; Capitani,Mariana Dutilh de; Tirapegui,Julio.
Recent studies of the effects of physical exercise and caloric restriction have found several benefits on the metabolic and cardiovascular risk factors related to metabolic syndrome (MS). This review examines the current state of knowledge of the effects of physical exercise on the main pathologies associated with MS: obesity, insulin resistance, type 2 diabetes mellitus (DM2), dyslipidemias and hypertension. Although there are only a few randomized and controlled studies that evaluated the prevention and treatment of MS, strong evidence from controlled studies indicates that lifestyle changes that include regular physical exercise and caloric restriction are effective in preventing and treating DM2 in overweight individuals with reduced glucose tolerance....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical activity; Metabolic syndrome; Insulin/resistance; Type 2 diabetes mellitus; Obesity; Nutrition.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300003

Synergistic effect of sertraline and disulfiram against multidrug resistant bacteria as a new alternative to drug repositioning
Provedor de dados: 60
Autores: Serafin,Marissa Bolson; Bottega,Angelita; Foletto,Vitória Segabinazzi; Rosa,Taciéli Fagundes da; Rampelotto,Roberta Filipini; Carvalho,Fernanda Aguirre; Hörner,Rosmari.
The repositioning of approved drugs is atopic of interest for the academy and the pharmaceutical industry. The synergistic combination of these drugs can be successful in the treatment of infections caused by resistant bacteria. This study aimed to assess the in vitro synergistic antibacterial activity of sertraline and disulfiram and their interaction with ciprofloxacin and sulfamethoxazole/trimethoprim. We determined the minimum inhibitory concentration, the minimum bactericidal concentration and the fractional inhibitory concentration index. Eighteen bacterial strains were used, being nine American Type Culture Collection reference strains and nine multidrug resistant clinical isolates. Synergy was detected between sertraline and disulfiram against a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sertraline; Disulfiram.Antimicrobial activity; Drug synergism; Drug repositioning.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100548

Hepatoprotective effect of Alhagi sparsifolia against Alcoholic Liver injury in mice
Provedor de dados: 60
Autores: Maimaitimin,Kuerbanjiang; Jiang,Zhihui; Aierken,Aili; Shayibuzhati,Mikeremu; Zhang,Xiaoying.
Overconsumption of alcohol leads to alcoholic liver disease (ALD). Natural compounds have been investigated previously for their hepatoprotective activities against liver injury. This study investigated the protective effect of Alhagi sparsifolia on ALD. Alcohol was administered to mice for three consecutive days; either alone or in combination with Alhagi sparsifolia extract (150, 300, 600 mg/kg). Serum aspartate aminotransferase and alanine transaminase as biomarkers of liver injury, the content of malonaldehyde, hydrogen peroxide (H2O2) and glutathione which indicated the redox status of liver and the antioxidant enzyme activity of super oxide dismutase were detected, respectively. Moreover, the expression of protein cytochrome P450 2E1 (CYP2E1) the key...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholic liver disease; Alhagi sparsifolia; Hepatoprotective effect; Antioxidantion; CYP2E1.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300625

Synthesis and  in silico study of 2-furyl(4-{4-[(substituted)sulfonyl]benzyl}-1-piperazinyl)methanone derivatives as suitable therapeutic agents
Provedor de dados: 60
Autores: Hussain,Ghulam; Abbasi,Muhammad Athar; Aziz-ur-Rehman,; Siddiqui,Sabahat Zahra; Shah,Syed Adnan Ali; Ashraf,Muhammad; Qurat-ul-Ain,; Ahmad,Irshad; Malik,Rabia; Lodhi,Muhammad Arif; Khan,Farman Ali; Shahid,Muhammad; Fatima,Hina.
Abstract In the study presented here, a new series of 2-furyl(4-{4-[(substituted)sulfonyl]benzyl}-1-piperazinyl)methanone derivatives was targeted. The synthesis was initiated by the treatment of different secondary amines (1a-h) with 4-bromomethylbenzenesulfonyl chloride (2) to obtain various 1-{[4-(bromomethyl)phenyl]sulfonyl}amines (3a-h). 2-Furyl(1-piperazinyl)methanone (2-furoyl-1-piperazine; 4) was then dissolved in acetonitrile, with the addition of K2CO3, and the mixture was refluxed for activation. This activated molecule was further treated with equi-molar amounts of 3a-h to form targeted 2-furyl(4-{4-[(substituted)sulfonyl]benzyl}-1-piperazinyl)methanone derivatives (5a-h) in the same reaction set up. The structure confirmation of all the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piperazine derivatives/antimicrobial activity; Piperazine derivatives/in silico; Piperazine derivatives/Cholinesterase assays; Piperazine derivatives/ hemolytic activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100613

Biological activities of the fermentation extract of the endophytic fungus Alternaria alternata isolated from Coffea arabica L.
Provedor de dados: 60
Autores: Fernandes,Maurette dos Reis Vieira; Silva,Tales Alexandre Costa e; Pfenning,Ludwig Heinrich; Costa-Neto,Cláudio Miguel da; Heinrich,Tassiela Andréa; Alencar,Severino Matias de; Lima,Marisa Aparecida de; Ikegaki,Masaharu.
A total of 22 endophytic fungi isolated from coffee (Coffea arabica L.) were cultivated in vitro and their crude extracts tested. The screening was carried out using the agar diffusion method against Staphylococcus aureus, Escherichia coli and Candida albicans. The most effective isolate was Alternaria alternata, and subsequently, its extract was assayed. The total phenolic content was 3.44 μg GAE/mg of the crude extract. For the antibacterial and antifungal activity assays, minimum inhibitory concentrations (MIC) and minimum bactericidal and fungicidal concentrations (MBC and MFC) were determined. The ranges of MIC values were 50-100 μg/mL for S. aureus and 400-800 μg/mL for E. coli. The extract did not show activity in the tested concentrations for C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic fungi; Bioactive compounds; Alternaria alternata/extract/antioxidant activity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400010

Maprotiline treatment differentially influences cardiac β-adrenoreceptors expression under normal and stress conditions
Provedor de dados: 60
Autores: Spasojevic,Natasa; Jovanovic,Predrag; Dronjak,Sladjana.
Alterations in cardiac function were observed in antidepressants treated patients and published in several clinical reports. These detected changes could be either a consequence of the treatment or of depression itself, which has already been proved to be a risk factor in heart diseases. In order to determine a possible influence of chronic treatment with norepinephrinergic reuptake inhibitor, maprotiline, on the heart, we investigated gene expression of cardiac β-adrenoceptors both in controls and in animals with signs of depression. The rats were divided into two groups, unstressed controls and those exposed to chronic unpredictable mild stress (CUMS). The groups were further divided into two subgroups, one receiving daily intraperitoneal injections of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antidepressants; Depression/treatment; Cardiovascular diseases/associated with the use of antidepressants; SS-adrenoceptors/gene expression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400023

Formulation, optimization, and characterization of snakehead fish (Ophiocephalus Striatus) powder nanoemulgel
Provedor de dados: 60
Autores: Tungadi,Robert; Wicita,Prisca.
The aim of the present study was to characterize and evaluate nanoemulgel of snakehead fish powder (SFP) for the poorly water-soluble drug. SFP was formulated into nanoemulsion utilizing the best comparison of surfactant, co-surfactant, and oil. Diverse nanoemulsion components (oil, surfactant, and co-surfactant) were chosen based on solvency and emulsification capacity. SFP 0.1% loaded nanoemulsion which tested by stress-stability testing which carried out for all formulations and those that passed these tests were characterized for droplet size, polydispersity index (PDI), zeta potential, pH, viscosity, and transmittance. After that, nanoemulsion was added with 1.5%, 2.0%, and 2.5% of HPMC in different concentrations and mixed until nanoemulgel form and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoemulgel; Nanoemulsion; Powder; Particle size analyzer; Snakehead fish.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100503

Analytical lifecycle management oriented development and validation of a new stability-indicating ultra-fast liquid chromatographic method: case study of Glycopyrrolate
Provedor de dados: 60
Autores: Panda,Sagar Suman; Kumar,B. V. V. Ravi; Swain,Suchismita.
A new stability-indicating liquid chromatographic method was developed and validated for the estimation of glycopyrrolate in pharmaceutical formulations. A contemporary approach to analytical life-cycle management was followed to develop a robust and reliable chromatographic method. Scouted method variables such as % methanol, the strength of tetra butyl ammonium hydrogen sulfate and mobile phase flow rate were optimized using the design of experiment approach and their effect on critical quality attributes was studied. The critical quality attributes viz. retention time, theoretical plate count and symmetry factor were highly influenced by the three critical method variables. Optimum chromatography was attained on a C-18 column with a mobile phase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycopyrrolate; Stability-indicating; Liquid chromatography; Life cycle management; Validation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100605

Development and validation of stability indicating liquid chromatographic (RP-HPLC) method for estimation of ubidecarenone in bulk drug and formulations using quality by design (QBD) approach
Provedor de dados: 60
Autores: Muheem,Abdul; Shakeel,Faiyaz; Zafar,Sobiya; Jahangir,Mohammed Asadullah; Warsi,Musarrat Husain; Jain,Gaurav Kumar; Ahmad,Farhan Jalees.
ABSTRACT A novel, accurate, precise and economical stability indicating Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method, was developed and validated for the quantitative determination of ubidecarenone (UDC) in bulk drug, UDC marketed formulation and UDC loaded cubosomes (CBMs) nanocarriers through Response surface methodology (RSM) design with three factors and three levels was performed to optimize the chromatographic variables followed by forced degradation studies of UDC were performed to detect degradation peak. RP-HPLC separation was achieved using mobile phase consisting of Acetonitrile:Tetrahydrofuran:Deionised water in the ratio 55:42:3 and a flow rate of 1.0 mL/min was optimized with a standard retention time (Rt) of 2.15...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High Performance Liquid Chromatography/validation/stability; Response surface methodology (RSM); Ubidecarnenone (UDC)/evaluation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400614

A qualitative study exploring perspectives towards rational use of medicines in Pakistan's Malaria Control Program (MCP)
Provedor de dados: 60
Autores: Malik,Madeeha; Hassali,Mohamed Azmi; Shafie,Asrul Akmal; Hussain,Azhar.
Malaria is one of the most important global public health problems threatening the health of the population owing to prevailing socio-economic conditions and epidemiological reasons in Pakistan. This qualitative study has focused on the perspectives held towards the rational use of medicine intervention among malaria control program officials. Eight semi-structured interviews with all officials working for the malaria control program in Islamabad were conducted. The interviews, which were audio-taped and transcribed verbatim, were evaluated by thematic content analysis and by all authors. All respondents agreed on successful implementation of the malaria control program in Pakistan for controlling malaria by improving diagnostic and treatment facilities...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Malaria Control Program; Drug/rational use; Intervention; Pakistan.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200014

Glycodendrimers: versatile tools for nanotechnology
Provedor de dados: 60
Autores: Roy,René; Shiao,Tze Chieh; Rittenhouse-Olson,Kate.
Combining nanotechnology with glycobiology has triggered an exponential growth of research activities in the design of novel functional bionanomaterials (glyconanotechnology). More specifically, recent synthetic advances towards the tailored and versatile design of glycosylated nanoparticles namely glyconanoparticles, considered as synthetic mimetics of natural glycoconjugates, paved the way toward diverse biomedical applications. The accessibility of a wide variety of these structured nanosystems, in terms of shapes, sizes, and organized around stable nanoparticles have readily contributed to their development and applications in nanomedicine. In this context, glycosylated gold-nanoparticles (GNPs), glycosylated quantum dots (QDs), fullerenes, single-wall...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimer; Glycodendrimer; Vaccine; Bacteria; Nanoparticles; E. coli; Lanthanides.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700008

Development, characterization and antioxidant activity of polysorbate based O/W emulsion containing polyphenols derived from Hippophae rhamnoides and Cassia fistula
Provedor de dados: 60
Autores: Khan,Barkat Ali; Akhtar,Naveed; Khan,Haroon; Braga,Valdir de Andrade.
The objective of this study was to develop a pharmaceutical O/W emulsion containing plant-derived polyphenol extracts and evaluate its stability and antioxidant activity. O/W emulsions were prepared using ionic surfactant polysorbate 80 (Tween 80®). The odorwas adjusted with few drops of blue sea fragrance. DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was used to evaluate the antioxidant activity of the plant extracts alone and emulsions containing these extracts. Physical stability was assessed by submitting the emulsions to storage at 8 ºC, 25 ºC, 40 ºC and 40 ºC + 70% RH (relative humidity) for two months. Various physical characteristics of emulsions monitored, include color, creaming, liquefaction, centrifugation and pH. Brookfield rotational rheometer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hippophae rhamnoids/pharmacognosy; Cassia fistula/pharmacognosy; Emulsion O/W; Emulsion O/topical use; Emulsion O/W; Emulsion O/conductivity; Emulsion O/W; Emulsion O/rheology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400016

Physical and chemical quality, biodiversity, and thermodynamic prediction of adhesion of bacterial isolates from a water purification system: a case study
Provedor de dados: 60
Autores: Alves,Roberta Barbosa Teodoro; Andrade,Nélio José de; Fontes,Edimar Aparecida Filomeno; Bernardes,Patrícia Campos; Carvalho,Antônio Fernandes de.
ABSTRACT The objective of this study was to evaluate the quality of water purification system and identify the bacteria this system, predict bacterial adherence according to the hydrophobicity of these microorganisms and of the polypropylene distribution loop for purified water. The assessment of drinking water that supplies the purification system allowed good-quality physical, chemical, and microbiological specifications. The physicochemical specifications of the distributed purified water were approved, but the heterotrophic bacteria count was higher than allowed (&gt;2 log CFU mL-1).The sanitation of the storage tank with chlorine decreased the number of bacteria adhered to the surface (4.34 cycles log). By sequencing of the 16SrDNA genes, six species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Purified water/quality; Purified water/adhesion; Purified water/bacterial biodiversity; Prediction thermodynamics; Hydrophobicity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200606

Potency of nano-antibacterial formulation from Sargassum binderi against selected human pathogenic bacteria
Provedor de dados: 60
Autores: Moni,Sivakumar Sivagurunathan; Alam,Mohammad Firoz; Safhi,M M.; Jabeen,Aamena; Sanobar,Syeda; Siddiqui,Rahimullah; Moochikkal,Remesh.
Seaweeds constitutes an abundant marine reserve that can be harnessed as source of new pharmaceutical agents. Sargassum binderi Sonder ex J. Agardh is a brown seaweed that is predominantly available from December to March in the Red Sea, Jazan, Kingdom of Saudi Arabia (KSA). In this study, three extracts were isolated using three different techniques, and were subjected to antibacterial assay. The petroleum ether extract of Sargassum binderi was more effective against selected human pathogenic bacteria than the other extracts. Therefore, further studies were focused on developing oleic acid vesicles entrapped with the petroleum ether extract of Sargassum binderi, with the aim of enhancing its penetration property. Oleic acid vesicles were prepared by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red sea; Sargassum species; Fatty acid vesicles; Antibacterial formulations.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400628

Biological properties of water-soluble phosphorhydrazone dendrimers
Provedor de dados: 60
Autores: Caminade,Anne-Marie; Turrin,Cédric-Olivier; Majoral,Jean-Pierre.
Dendrimers are hyperbranched and perfectly defined macromolecules, constituted of branches emanating from a central core in an iterative fashion. Phosphorhydrazone dendrimers constitute a special family of dendrimers, possessing one phosphorus atom at each branching point. The internal structure of these dendrimers is hydrophobic, but hydrophilic terminal groups can induce the solubility of the whole structure in water. Indeed, the properties of these compounds are mainly driven by the type of terminal groups their bear; this is especially true for the biological properties. For instance, positively charged terminal groups are efficient for transfection experiments, as drug carriers, as anti-prion agents, and as inhibitor of the aggregation of Alzheimer's...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimers; Phosphorhydrazone dendrimers; Biological properties; Nanomedicine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700004

Evaluation of the protective effects of tocotrienol-rich fraction from palm oil on the dentate gyrus following chronic restraint stress in rats
Provedor de dados: 60
Autores: Talip,Saiful Bhari; Asari,Mohd Asnizam; Khan,Aaijaz Ahmed; Sirajudeen,Kuttulebbai Nainamohamed Salam.
Exposure to chronic restraint stress has been shown to cause a number of morphological changes in the hippocampal formation of rats. Tocotrienol, an isoform of vitamin E, exhibits numerous health benefits, different from those of tocopherol. Recent studies have demonstrated that tocotrienol prevents stress-induced changes in the gastric mucosa, thus indicating that it may also protect other organs such as the brain from the damaging effects of stress. Therefore, the aim of the present study was to investigate the protective effect of tocotrienol-rich fraction (TRF) extracted from palm oil on the dentate gyrus of rats following exposure to chronic restraint stress. Thirty-six male Sprague Dawley rats were divided into four groups: control, stress,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tocotrienol; Tocotrienol rich fraction; Restraint stress; Dentate gyrus; Hippocampus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200020

In vitro dissolution kinetic for mycophenolic acid derivatives tablets
Provedor de dados: 60
Autores: Costa,André Lima de Oliveira; Enéas,Paula Cristina Rezende; Miranda,Tiago Assis; Mingoti,Sueli Aparecida; Soares,Cristina Duarte Vianna; Pianetti,Gerson Antônio.
Mycophenolate mofetil (MMF) and mycophenolate sodium (MPS) are an ester and a salt of mycophenolic acid. They have different kinetic in vivo characteristics due to differences in molecular structures, physicochemical properties and formulations administered. In this study, dissolution profiles of reference products were tested in different media to evaluate the effect of pH, kinetic dissolution and the best statistical model that can be used to predict the release of both drugs. The drug release was determined by using a validated ultraviolet spectrophotometry method, λ 250 nm. The method showed to be selective, linear, precise and accurate for MMF in 0.1 M HCl and MPS in sodium phosphate buffer pH 6.8. Dissolution kinetics models of zero order, first...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycophenolate sodium; Mycophenolate mofetil; Dissolution profiles; Weibull kinetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200013

BJPS as strategical for Pharmaceutical Sciences
Provedor de dados: 60
Autores: Ferreira,Elizabeth Igne.
Tipo: Info:eu-repo/semantics/article
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100001

In vitro antifungal activity and phytochemical characterization of Eugenia uniflora, Libidibia ferrea and Psidium guajava
Provedor de dados: 60
Autores: Ferreira,Magda Rhayanny Assunção; Santiago,Rosilene Rodrigues; Silva-Rocha,Walicyranison Plínio; Souza,Luanda Barbara Ferreira Canário de; Faria,Maria Graciela Icher; Mello,João Carlos Palazzo de; Langassner,Silvana Maria Zucolotto; Chaves,Guilherme Maranhão; Milan,Eveline Pipolo; Svidzinski,Terezinha Inez Estivalet; Soares,Luiz Alberto Lira.
The aim of this study was evaluate the in vitro antifungal activity of crude extracts from Eugenia uniflora, Libidibia ferrea and Psidium guajava. The extracts were obtained by turbo-extraction using water (AQ) or acetone-water (AC-W) (7:3, v/v) as solvents and lyophilized to obtain the crude extracts (CE). The CE were characterized by UV-Vis, TLC and HPLC. The activity of CEs was investigated against clinical isolates of Candida spp. and the Minimum Inhibitory Concentration (MIC), MIC50 and MIC90 were determinated. The analysis by TLC showed that all CEs presented polyphenols (flavonoids and tannins). The CEs from E. uniflora showed higher amount of polyphenols (30.35 ± 2.15%, AC-W) and the HPLC analysis revealed the tannins in all extracts. The CEs of E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Herbal extracts; Polyphenols; Candida isolates; Oral candidiasis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100608

Exploring the natural chemiome to target interleukin-6 receptor (IL-6R) cytokines: an atomic scale investigation for novel rheumatoid arthritis drug discovery
Provedor de dados: 60
Autores: Chen,Qiujian; Niu,Xiaohui; Li,Nana.
ABSTRACT Natural compounds are a gold mine for treating rheumatoid arthritis (RA). The etiology of this disease is linked to inflammation, where cytokines play an important role. Strategies have been drafted for targeting cytokines as a therapeutic option in patients with RA. Inhibiting cytokines with natural compounds has become a major focus for the development of drugs to treat RA. Here, a structure-based drug design approach was employed to identify novel leads to target the interleukin 6 receptor (IL-6R). A total of 48,531 compounds of natural origin were screened. Two of these compounds were shortlisted for molecular docking simulation and tested for inhibiting gp130 dimerization in human macrophages. The results show that Lead5 (CID5329098)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis/treatment/natural compounds; Cytokines/therapeutic target; Docking; Natural compounds/study.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300627

Influence of radiation on endotoxin test using the PTS TM for 18-FDG radiopharmaceutical
Provedor de dados: 60
Autores: Santos-Oliveira,Ralph.
F-18 FDG (2-[18-F] fluoro-2-deoxy-D-glucose) is the most frequently used radiopharmaceutical for PET and PET CT imaging exams. The FDA recently approved the use of the PTS TM (Portable Test System) as an alternative to the standard test proposed by the United States Pharmacopeia using the LAL (Limulus Amebocyte Lysates), that takes longer to perform (about 1h) than the PTS TM (15 min). Recent studies have demonstrated that radiation could interfere with the PTS TM test. In order to study the effects of radiation on the PTS TM test and/or equipment, 27 batches of F-18 FDG produced in the Nuclear Engineering Institute were analyzed. The results showed that no direct correlation with radiation was found in any of the cases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 18-FDG/utilization; Radiopharmaceuticals; Nuclear medicine; Endotoxin test; Portable Test System/radiation effects.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300019

Antinociceptive activity of Thyme (Thymus vulgaris L.) and interactions with neurotropics and analgesics
Provedor de dados: 60
Autores: Rašković,Aleksandar Lazar; Kvrgić,Maja Petar; Tomas,Ana Drago; Stilinović,Nebojša Petar; Čabarkapa,Velibor Spasoje; Stojšić-Milosavljević,Anastazija Ðurica; Kusturica,Milica Nada Paut; Rakić,Dušica Branko.
The plant world represents an important source of potential therapeutic agents, but concomitant administration of herbal and conventional medications may result in interactions with subsequent beneficial or adverse effects. This study was designed to examine the analgesic effect of thyme tincture and thyme syrup, two commonly used thyme formulations, and their interactions with codeine, paracetamol, pentobarbital and diazepam in mice. The identification and quantification of thymol and carvacrol were carried out by GC/MS and GC/FID. The analgesic activity was studied using a hot plate method. Effects of thyme syrup on diazepam-induced motor coordination impairment in rotarod test and on pentobarbital-induced sleeping time were also determined. Thymol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thymus vulgaris; Hot plate; Analgesic; Interactions; Pentobarbital; Diazepam.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100634

In vitro/in vivo performance of different complexes of itraconazole used in the treatment of vaginal candidiasis
Provedor de dados: 60
Autores: Mirza,Mohammad Aamir; Rahman,Mohammad Akhlaquer; Talegaonkar,Sushama; Iqbal,Zeenat.
A large majority of new chemical entities and many existing drug molecules exhibit poor aqueous solubility, which may limit their potential use in developing drug formulations, with optimum bioavailability. One of the approaches to improve the solubility of a poorly water soluble drug and eventually its bioavailability is complexation with agents like humic acid (HA), fulvic acid (FA), β-cyclodextrin (β-CD), 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and caffeine (Caff). The current work emphasized at employing these agents to prepare different complexes and their in vitro/in vivo assessment. All the complexes evaluated for their complexation efficiency and authenticated by molecular modeling; conformational analysis, differential scanning calorimetry (DSC),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Itraconazole/complexes; Itraconazole/evaluation; Itraconazole/complexes; Itraconazole/in vitro; Itraconazole/in vivo performance; Vaginal candidiasis/treatment; Drugs/solubility; Drugs/formulation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400020

Phage therapy: progress in pharmacokinetics
Provedor de dados: 60
Autores: Qadir,Muhammad Imran; Mobeen,Tahira; Masood,Ardas.
ABSTRACT The concept of phage therapy exists in the history and it has been ignored for a long time, but the consequence of drug resistance in pathogen bacteria has forced the forgotten kingdom of phage therapy to be re-explored. However, for the successful implementation and acceptance of phage therapy worldwide, the number of factors need to be addressed. In pharmacology of phage therapy, pharmacodynamics is a straightforward concept, on the other hand, owing to the unique feature of phages to replicate and their high sensitivity, pharmacokinetics is rather complex. In this review, we have discussed pharmacokinetics and some recent advances in delivery systems as to achieve the therapeutically effective concentrations of phage in their activated form.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phage therapy/pharmacology; Pharmacokinetics; Delivery systems.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100402

Antiinflammatory, antinociceptive and antipyretic effects of hydroethanolic extract from Macrosiphonia velame (A. St.-Hil.) M. Arg. in animal models
Provedor de dados: 60
Autores: Ribeiro,Reginaldo Vicente; Silva,Regilane Matos da; Lima,Joaquim Corsino da Silva; Martins,Domingos Tabajara de Oliveira.
Macrosiphonia velame (Apocynaceae), popularly known as "velame-branco", is mainly used for treating inflammatory conditions. The antiinflammatory, antinociceptive and antipyretic effects of the hydroethanolic extract of the xylopodium from M. velame (HEMv) were evaluated using several animal models. HEMv showed low acute oral toxicity with LD50 of 4.176 ± 218.5 mg/kg in mice. In tests of carrageenan and dextran-induced paw edema and carrageenan-induced pleurisy in rats, and croton oil-induced cutaneous dermatitis in mice, HEMv presented systemic and topical antiinflammatory activities. In experiments of nociception induced by acetic acid, formalin and capsaicin in mice, the HEMv evidenced an antinociceptive effect, being active against both inflammatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrosiphonia velame/antiinflammatory effect; Macrosiphonia velame/antinociceptive effect; Macrosiphonia velame/antipyretic effect; Macrosiphonia velame/pharmacognosy; Macrosiphonia velame/experimentel study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300015

Pharmacogenetic implications in the management of metabolic diseases in Brazilian populations
Provedor de dados: 60
Autores: Hirata,Rosario Dominguez Crespo; Cerda,Alvaro; Genvigir,Fabiana Dalla Vecchia; Hirata,Mario Hiroyuki.
Dyslipidemia, diabetes, obesity and hypertension are common metabolic diseases. In the last decades, unhealthy lifestyle and aging have leads to an increased incidence of these diseases, increasing morbidity and mortality by cardiovascular causes. The treatment of metabolic diseases includes life-style interventions as healthy diet and physical exercise, as well as pharmacological interventions. Several drugs are available for the management of metabolic diseases including among others lipid-lowering antidiabetics and antihypertensive drugs. Variability in response to these drugs is influenced by both genetic and non-genetic factors. Polymorphisms in genes related to drug pharmacokinetics and pharmacodynamics have been shown to influence drug efficacy and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacogenetics; Metabolic diseases; Gene polymorphism; Drug response.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700404

Quality of life of HIV+ patients undergoing pharmacotherapeutic follow-up
Provedor de dados: 60
Autores: Loureiro,Catarine Vitor; Reis,Henry Pablo Lopes Campos e; Magalhães,Karla do Nascimento; Carmo,Cristiane Policarpo; Leite,Francisca Ana Maria; Passos,Ana Cláudia de Brito; Firmino,Paulo Yuri Milen; Kheir,Nadir; Woods,David John; Ponciano,Ângela Maria de Souza; Fonteles,Marta Maria de França.
The influence of pharmacotherapeutic follow-up (PTF) on quality of life was evaluated in 45 HIV+ patients, who were undergoing initial antiretroviral therapy at a specialized care center in northeast Brazil. PTF lasted nine months and quality of life was analyzed at the 1st and 9th meetings using a questionnaire validated for Brazil. The study identified 643 problems related to antiretrovirals and there were 590 pharmaceutical interventions during the PTF. The comparative analysis between the results of the 1st and the 9th meeting was statistically significant for all domains of the questionnaire. For asymptomatic patients, only one domain was statistically significant. For symptomatic patients, six domains were significant. Patients with one year of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacotherapeutic follow-up/influence; Pharmacotherapeutic follow-up/quality of life; HIV patients/quality of life; Pharmaceutical care.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400015

Stability evaluation of tocopheryl acetate and ascorbyl tetraisopalmitate in isolation and incorporated in cosmetic formulations using thermal analysis
Provedor de dados: 60
Autores: Almeida,Mariana Mandelli de; Lima,Cibele Rosana Ribeiro de Castro; Quenca-Guillen,Joyce Santos; Moscardini Filho,Elder; Mercuri,Lucildes Pita; Santoro,Maria Inês Rocha Miritello; Kedor-Hackmann,Erika Rosa Maria.
In view of the increase in the number of cosmetic preparations containing antioxidant vitamins, chiefly, due to their action in preventing the process of skin aging, there is a need to develop pre-formulation studies and to validate analytical methods in order to obtain high quality products. Thus, the objective of this research was to evaluate and compare the thermal behavior of tocopheryl acetate and ascorbyl tetraisopalmitate as raw materials, and incorporated into a base cream. Thermogravimetry (TG / DTG) and differential scanning calorimetry (DSC) were used for this purpose. Both vitamins were found to be stable up to 250ºC. The base cream (placebo) and the sample (base cream containing the vitamins) presented different weight loss. Thermal analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thermal analysis; Cosmetic emulsion/qualitative analysis; Cosmetics/quality control; Tocopheryl acetate/antioxidant action; Ascorbyl tetraisopalmitate/antioxidant action; Vitamins/use in cosmetics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100015

Solubility evaluation of didanosine: a comparison between the equilibrium method and intrinsic dissolution for biopharmaceutics classification purposes
Provedor de dados: 60
Autores: Dezani,André Bersani; Dezani,Thaisa Marinho; Ferreira,Julie Caroline Ferrari; Serra,Cristina Helena dos Reis.
ABSTRACT BCS (Biopharmaceutics Classification System) and BDDCS (Biopharmaceutics Drug Disposition Classification System) were proposed as tools for classifying drugs into four categories. Both systems consider the solubility as an important characteristic for the classification of compounds in drug development and in vivo disposition prediction. Although some results of drug solubility can be found in the literature, the aforementioned characteristic is not entirely clear when considering didanosine (ddI). Based on that, the solubility of ddI was evaluated using equilibrium and intrinsic dissolution methods. For the equilibrium method, excess amount of ddI was added to each media until obtaining a supersaturated solution and the mixture was submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Didanosine/solubility; Biopharmaceutics Classification System (BCS); Biopharmaceutics Drug Disposition Classification System (BDDCS); Intrinsic dissolution; Equilibrium solubility.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200632

Study of acute toxicity and investigation of the presence of β-N-methylamino-L-alanine in the Gunnera manicata L. a species native to Southern Brazil
Provedor de dados: 60
Autores: Mariotti,Kristiane de Cássia; Barreto,Fabiano; Schmitt,Gabriela Cristina; Zancanaro,Ivomar; Dallegrave,Eliane; Singer,Rodrigo Bustos; Leal,Mirna Bainy; Limberger,Renata Pereira.
Gunnera (Gunneraceae) forms a complex association with the cyanobacterium Nostoc puctiforme L. Gunnera-Nostoc symbiosis is the only one reported involving a flowering plant, and results in the formation of the neurotoxic amino acid β-N-methylamino-L-alanine (BMAA). The species Gunnera manicata L., for which phytochemical, pharmacological and toxicological studies are lacking, is found in Southern Brazil. Therefore, acute toxicity and the presence of neurotoxic amino acid were investigated in aqueous extracts of G. manicata. The acute toxicity test was conducted by administering aqueous root extract of G. manicata at a concentration of 2000 mg/kg in a single dose orally to Wistar rats. Lethality was monitored daily for 14 days after treatment. The relative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gunnera manicata L; /toxicity; Β-N-methylamino-L-alanine/identification; Neurotoxins/identification; Acute toxicity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300021

β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil
Provedor de dados: 60
Autores: Leite,Clariana Akemi Kariya; Oizumi,Karina Yoshimi; Caleffi-Ferracioli,Katiany Rizzieri; Scodro,Regiane Bertin de Lima; Pádua,Rubia Andreia Falleiros de; Cardoso,Rosilene Fressatti; Pires,Claudia Terencio Agostinho; Siqueira,Vera Lucia Dias.
ABSTRACT The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Pseudomonas aeruginosa; Acinetobacter spp; Antimicrobial resistance; Beta-lactamases; Bacterial typing; Intensive Care Unit/study/Brazil; Drug-resistant bacteria.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200609

Chemical review and studies related to species from the genus Tynanthus (Bignoniaceae)
Provedor de dados: 60
Autores: Cansian,Fernanda Colombi; Merino,Francis José Zortéa; Dias,Josiane de Fátima Gaspari; Zanin,Sandra Maria Warumby; Miguel,Obdulio Gomes; Miguel,Marilis Dallarmi.
Species from the Bignoniaceae Family, including the genus Tynanthus, are very prevalent in the tropical Americas, with specimens found in a large part of the Brazilian territory. These plants are commonly used in traditional medicine for several purposes, and some studies have described their chemical structure, in addition to other reports related to some species from this genus. This review aimed to gather information from published works concerning species of the genus Tynanthus, as well as to detect flaws in research related to these plants, which may have great biological and pharmaceutical importance. Also, this review points out some common chemical characteristics of these species, providing information that may help new researchers to improve...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tynanthus/pharmacognosy; Tynanthus/phytochemistry; Tynanthus/biological activity; Bignoniaceae/pharmacognosy; Bignoniaceae/phytochemistry; Medicinal plants.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300515

Synthesis of some novel enzyme inhibitors and antibacterial agents derived from 5-(1-(4-tosyl)piperidin-4-yl)-1,3,4-oxadiazol-2-thiol
Provedor de dados: 60
Autores: Sattar,Almas; Aziz-ur-Rehman,; Abbasi,Muhammad Athar; Siddiqui,Sabahat Zahra; Rasool,Shahid; Ahmad,Irshad.
ABSTRACT Keeping in mind the pharmacological importance of the 1,3,4-oxadiazole moiety, a series of new S-substituted derivatives, 5a-h, of 5-(1-(4-tosyl)piperidin-4-yl)-1,3,4-oxadiazol-2-thiol (3) were synthesized. The reaction of p-toluenesulfonyl chloride (a) and ethyl isonipecotate (b) produced ethyl 1-(4-tosyl)piperidin-4-carboxylate (1) which was further transformed into 1-(4-tosyl)piperidin-4-carbohydrazide (2) by hydrazine hydrate in methanol. Compound 2 was refluxed with CS2 in the presence of KOH to synthesize 5-(1-(4-tosyl)piperidin-4-yl)-1,3,4-oxadiazol-2-thiol (3). The desired compounds, 5a-h, were synthesized by stirring 3 with aralkyl halides, 4a-h, in DMF using NaH as an activator. The structures of synthesized compounds were elucidated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 1; 3; 4-Oxadiazole/antibacterial activity. 1; 3; 4-Oxadiazole/enzyme inhibitory activity. Isonipecotate. Sulfonamide.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100009

Comparative study of sustained-release lipid microparticles and solid dispersions containing ibuprofen
Provedor de dados: 60
Autores: Almeida,Hugo; Amaral,Maria Helena; Lobão,Paulo.
Ibuprofen is one of the most important non-steroidal anti-inflammatory drugs used in the treatment of inflammatory diseases. In its pure state, ibuprofen presents poor physical and mechanical characteristics and its use in solid dosage forms needs the addition of excipients that improve these properties. The selection of the best excipients and the most suitable pharmaceutical dosage form to carry ibuprofen is very important for the industrial success of this drug. Given these factors, lipid microparticles and solid dispersions of ibuprofen with cetyl alcohol, stearic acid, and hydrogenated castor oil were prepared. These formulations were intended to improve the physical and mechanical characteristics and to sustain the release of this drug. Physical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ibuprofen/solid dispersions; Ibuprofen/release profile; Ibuprofen/lipid microparticles; Ibuprofen/release profile; Excipients; Cetyl alcohol; Stearic acid; Hydrogenated castor oil.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300020

Enhancing the aqueous solubility and dissolution of olanzapine using freeze-drying
Provedor de dados: 60
Autores: Dixit,Mudit; Kini,Ashwini Gopalkrishna; Kulkarni,Parthasarthi Keshavarao.
The aim of the present study was to develop an olanzapine freeze-dried tablet (FDT). The solubility and dissolution rate of poorly water-soluble olanzapine was improved by preparing a freeze-dried tablet of olanzapine using the freeze-drying technique . The FDT was prepared by dispersing the drug in an aqueous solution of highly water-soluble carrier materials consisting of gelatin, glycine, and sorbitol. The mixture was poured in to the pockets of blister packs and then was subjected to freezing and lyophilisation. The FDT was characterised by DSC, XRD and SEM and was evaluated for saturation solubility and dissolution. The samples were stored in a stability chamber to investigate their physical stability. Results obtained by DSC and X-ray were analysed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freeze-dried tablets/development; Olanzapine/freeze-dried tablets; Olanzapine/solubility; Olanzapine/stability; Olanzapine/dissolution; Freeze-drying.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400011

Polymeric emulgel carrying Cinnamomum tamala extract: promising delivery system for potential topical applications
Provedor de dados: 60
Autores: Arshad,Wajeeha; Khan,Haji Muhammad Shoaib; Akhtar,Naveed; Mohammad,Imran Shair.
Currently, the use of natural compounds obtained from plants tremendously increased due to their promising therapeutic properties. The aim of this study was to formulate a stable emulgel formulation loaded with Cinnamomum tamala (CT) extract. The antioxidant activity of plant extract was determined by DPPH inhibition assay. The extract was successfully loaded into an emulgels using different concentrations of carbopol-940, liquid paraffin, emulsifying agents and preservatives. Preliminary stability study was performed of 17 CT emulgel formulations at accelerated temperature of 50 °C for 2-months. Organoleptic evaluation, centrifugation, globule size, pH, electrical conductivity and rheological studies were performed for a period of 90-days at different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cinnamomum tomala; Antioxidants; Emulgel; Physicak stability; Non-Newtonian; Pseudo-plasticity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100553

Therapeutic itinerary: trajectory for resolution of adverse events of patients using warfarin in Southern Brazil
Provedor de dados: 60
Autores: Colet,Christiane; Amador,Tânia Alves; Heineck,Isabela.
Warfarin is the most used anticoagulant in primary health care. Due to the narrow therapeutic index, its users are more susceptible to adverse events. The objective of this study was to describe the itinerary of the public health sector patients for resolution of adverse events related to warfarin. It is a prospective open cohort, held for a period of 18 months with warfarin users of the Brazilian public health system. Data were collected by monthly interviews and from patient records. Results: Sixty nine patients were interviewed, 64 of them completed monitoring and five died. Bleeding and venous thromboembolism were more frequent in patients starting treatment. It was observed that when adverse events have occurred, in most cases the patient held...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Warfarin/adverse drug; Cohort studies; Health services accessibility; Reaction; Brazil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300626

Development and validation of a dissolution method using HPLC for diclofenac potassium in oral suspension
Provedor de dados: 60
Autores: Rubim,Alexandre Machado; Rubenick,Jaqueline Bandeira; Laporta,Luciane Varini; Rolim,Clarice Madalena Bueno.
The present study describes the development and validation of an in vitro dissolution method for evaluation to release diclofenac potassium in oral suspension. The dissolution test was developed and validated according to international guidelines. Parameters like linearity, specificity, precision and accuracy were evaluated, as well as the influence of rotation speed and surfactant concentration on the medium. After selecting the best conditions, the method was validated using apparatus 2 (paddle), 50-rpm rotation speed, 900 mL of water with 0.3% sodium lauryl sulfate (SLS) as dissolution medium at 37.0 ± 0.5°C. Samples were analyzed using the HPLC-UV (PDA) method. The results obtained were satisfactory for the parameters evaluated. The method developed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dissolution method/development; Dissolution method/validation; Diclofenac potassium/release in oral suspensions; Oral suspension/quality control; High performance liquid chromatography/qualitative analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200423

Effectiveness of home pharmaceutical interventions in metabolic syndrome: a randomized controlled trial
Provedor de dados: 60
Autores: Azevedo,Maria da Glória Batista de; Pedrosa,Roseana Souza; Aoqui,Caroline Mapurunga; Martins,Rand Randall; Nagashima Junior,Toshiyuki.
ABSTRACT Metabolic syndrome (MetS) is a group of different risk factors that raises the chances of develop several health problems such, as cardiovascular disease and diabetes. There are few cases in literature of studies that specifically address the use of pharmaceutical care in MetS. The aim of study was to evaluate the effectiveness of home pharmaceutical interventions in patients with this syndrome. The randomized clinical trial was conducted in subjects with diagnosis of MetS treated in a basic unit of health from Cuité, Paraíba. The patients were randomized in a 1:1 ratio to two groups: intervention (IG) and control (CG). The IG received monthly individual pharmaceutical interventions, while the CG did not. The effectiveness of the intervention...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome/study; Pharmaceutical care/home; Pharmaceutical interventions; Drug related problems.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200608

QuEChERS-HPLC-DAD method for sulphonamides in chicken breast
Provedor de dados: 60
Autores: Machado,Simone Caetani; Landin-Silva,Mariane; Maia,Patrícia Penido; Rath,Susanne; Martins,Isarita.
The development of a QuEChERS-HPLC-DAD method using a Lichrospher 60 RP-Select B column (250 x 4.6 mm x 5 µm) at 40ºC, mobile phase constituted by phosphate buffer:acetonitrile (75:25, v/v) at a initial flow rate of 0.5 mL min-1, increased by 1.2 mL min-1 and at 265 nm is presented for simultaneous determination of sulphadiazine, sulphametoxipiridazine and sulphamethoxazole in chicken breast samples. QuEchERS is inexpensive, fast and easy, and the extraction of the analytes of the matrix was successfully employed. In addition, the method presented linearity, in the range of 25, 50, 100, 150, 175, and 200 µg kg-1, precision, selectivity and sensitivity. The intraday precision (RSD %) for QuEChERS method was between 3.6-10.8 (SDZ), 6.9-14.1 (SPZ) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QuEChERS; Sulphonamides determination; Veterinary drugs residues; Chicken breast; HPLC-DAD.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100017

Microemulsion based on Pterodon emarginatus oil and its anti-inflammatory potential
Provedor de dados: 60
Autores: Pascoa,Henrique; Diniz,Danielle Guimarães Almeida; Florentino,Iziara Ferreira; Costa,Elson Alves; Bara,Maria Teresa Freitas.
This article reports the development of a pharmaceutical product containing vegetable actives from a Brazilian medicinal plant. The possibility of forming a microemulsion using Pterodon emarginatus ("sucupira") oil was evaluated and the anti-inflammatory potential of this microemulsion was also examined. A formulation was developed using P. emarginatus oil, a mixture of ethoxylated Castor Oil (Ultramone(r) R-540/propylene glycol 2:1) (surfactant/cosurfactant) and distilled water at a ratio of 10:15:75, respectively. The microemulsion which was selected was then subjected to the preliminary stability test and analyzed in terms of average diameter of droplets, pH, zeta potential, and polydispersity index, on the 1st, 7th, 15th, and 30th days after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pterodon emarginatus; Pterodon emarginatus; Medicinal plants; Microemulsion; Phytomedicine; Pharmaceutical technology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100117

Quantitation of artemether in pharmaceutical raw material and injections by high performance liquid chromatography
Provedor de dados: 60
Autores: César,Isabela da Costa; Pianetti,Gerson Antônio.
The quantitation of artemether in both pharmaceutical raw material and injections was carried out by high performance liquid chromatography (HPLC) with ultraviolet detection. A Zorbax C18 column (150 x 4.6 mm; 5 μm), at 30 °C, and a mobile phase composed of acetonitrile and water (70:30), at a flow rate of 1ml/min, were used. The detection wavelength was 216 nm and the injection volume was 20 μL. The method proved to be linear (r²=0.9999), precise (RSD &lt; 20% for intra-day and inter-day precision), accurate and selective regarding possible impurities and excipients of the samples. The detection and quantitation limits were 8 μg/mL and 25 μg/mL, respectively. The artemether content obtained in the raw material analysis was 99.26% and in the injections,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemether/quantitation; High performance liquid chromatography/quantitative analysis; Antimalarials.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400018

Validation of an analytical method by high-performance liquid chromatography and microbiological assay, biological safety and in silico toxicity for danofloxacin
Provedor de dados: 60
Autores: Cordeiro,Everson Willian Fialho; Hilgert,Renata Medeiros; Batista,Luiz Alcides das Chagas; Souza,Raul Oliveira; Oliveira,Luís Flávio Souza de; Machado,Michel Mansur; Malesuik,Marcelo Donadel; Paula,Fávero Reisdorfer; Stopiglia,Cheila Denise Ottonelli; Paim,Clésio Soldateli.
Danofloxacin is a veterinary fluoroquinolone used to treat respiratory and gastrointestinal diseases of birds, pigs and cattle. The literature reviewed shows some analytical methods to quantify this fluoroquinolone, but microbiological and biological safety studies are limited. The analytical methods were validated by the Official Codes. The LC-DAD method was developed and validated using an RP-18 column, mobile phase containing a mixture of 0.3% triethylamine (pH 3.0) and acetonitrile (85:15, v/v). The microbiological assay was performed by agar diffusion method (3 x 3) and Staphylococcus epidermidis as a microorganism test. Forced degradation studies were performed in both methods. The minimum inhibitory concentration (MIC) was performed by test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Danofloxacin; Microbiological assay; Biological safety; In silico toxicology; Fluoroquinolones photodegradation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100555

Production of intravenous human dengue immunoglobulin from Brazilian-blood donors
Provedor de dados: 60
Autores: Gouveia,Frederico Leite; Oliveira,Renata Mendes Batista de; Lucena,Antônio Edson de Souza; Cordeiro,Marli Tenório; Leite,Ana Cristina Lima.
Dengue represents an important health problem in Brazil and therefore there is a great need to develop a vaccine or treatment. The neutralization of the dengue virus by a specific antibody can potentially be applied to therapy. The present paper describes, for the first time, the preparation of Immunoglobulin specific for the dengue virus (anti-DENV IgG), collected from screened Brazilian blood-donations. Production was performed using the classic Cohn-Oncley process with minor modifications. The anti-DENV IgG was biochemically and biophysically characterized and fulfilled the requirements defined by the European Pharmacopoeia. The finished product was able to neutralize different virus serotypes (DENV-1, DENV-2, and DENV-3), while a commercial IgG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Viral infections; Dengue/treatment; Dengue/virus; Dengue/neutralization; Immunoglobulins/preparation; Plasma fractionation; Virus neutralization.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400022

Independent and synergistic activity of the flavonoids of Gracilaria corticata as promising antidiabetic agents
Provedor de dados: 60
Autores: Sangeetha,Ragupathy.
The therapeutic approaches for Type 2 Diabetes Mellitus rely most on the usage of oral hypoglycaemic drugs. These drugs have adverse side effects and hence alternative medicines are continuously explored. The present study intends to investigate the antidiabetic potential of the flavonoids present in Gracilaria corticata. The flavonoids were isolated (FEGC) and their inhibitory activity on the carbohydrate hydrolysing enzymes such as α-amylase and α-glucosidase was analysed. The flavonoids were found to inhibit α-amylase and α-glucosidase with an IC50 value of 302 µg and 75 µg respectively. The synergistic effect of FEGC and luteolin was also investigated and the results show that both FEGC and luteolin inhibited synergistically at half their IC50 values....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-amylase; Α-glucosidase; Antidiabetic; Gracilaria corticata; Luteolin; Synergistic.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100618

Micro and nanocrystalline cellulose based oral dispersible film; preparation and evaluation of in vitro/in vivo rapid release studies for donepezil
Provedor de dados: 60
Autores: AnjiReddy,Keshireddy; Karpagam,Subramanian.
Oral fast-dispersible film was prepared by utlizing donepezil hydrochloride (drug) and various cellulose derivatives such as hydroxypropyl methyl cellulose (hypermellose) (HPMC), microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC) to treat Alzheimer’s disease. NCC was synthesized by ultra-sonication method using MCC and this was converted to thinfilm formulation (NCC-F) using solvent casting technique. The interaction between the polymer and the drug was investigated by spectral analysis such as UV, FTIR, and 1H- NMR. FTIR confirmed that the compatibility of drug and polymer in ODF formulation. NCC-F has shown an average surface roughness of 77.04 nm from AFM and the average particle size of 300 nm from SEM analysis. Nano sized particle of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral dispersible film; Nanocrystalline cellulose; Ultrasonication; 3D atomic force microscopy; Donepezil; Solvent casting; Alzheimer’s disease.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100586

Determinação quantitativa de taninos em três espécies de Stryphnodendron por cromatografia líquida de alta eficiência
Provedor de dados: 60
Autores: Lopes,Gisely Cristiny; Sanches,Andréia Cristina Conegero; Toledo,Cleyton Eduardo Mendes de; Isler,Ana Cristina; Mello,João Carlos Palazzo de.
Um método de separação e quantificação por cromatografia líquida de alta eficiência (CLAE) em fase reversa foi desenvolvido usando água (0,05% de TFA):acetonitrila (0,05% de TFA) como fase móvel, em sistema gradiente para a análise dos flavan-3-óis presentes em extrato semipurificado das cascas de Stryphnodendron adstringens, Stryphnodendron polyphyllum e Stryphnodendron obovatum. A CLAE foi realizada com a fração acetato de etila (FAE) sendo submetida à extração em fase sólida (cartucho C18-E) com metanol:água (2:8), filtrada por membrana de porosidade 0,5 μm; a pré-coluna e coluna empregadas foram Phenomenex® Gemini C-18 (5 μm), com esta última mantida a 30 ºC, com vazão de 0,8 mL/min e detecção a 210 nm. Utilizaram-se soluções dos padrões de ácido...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stryphnodendron adstringens; Stryphnodendron polyphyllum; Stryphnodendron obovatum; Cromatografia líquida de alta eficiência; Taninos/determinação quantitativa; Análise quantitativa.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100017

The correlation between the phenolic composition and biological activities of two varieties of Brazilian propolis (G6 and G12)
Provedor de dados: 60
Autores: Cabral,Ingridy Simone Ribeiro; Oldoni,Tatiane Luiza Cadorin; Alencar,Severino Matias de; Rosalen,Pedro Luiz; Ikegaki,Masaharu.
Biological assays that have been performed on different types of Brazilian propolis have shown that type 6 propolis (G6) has a strong antimicrobial activity and a low flavonoid content. This study aimed to evaluate the correlation between the phenolic composition and the biological activities displayed by propolis G6 from the state of Bahia and green propolis, also known as type 12 (G12). The values of the flavonoids and the total phenolics in propolis G6 were different than those in propolis G12. Although the G12 variety presented greater antioxidant activity, propolis G6 proved to have greater antimicrobial and cytotoxic activities. The results showed that the phenolic compounds may not be the only compounds responsible for the biological activity. More...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian própolis/evaluation; Flavonoids/determination in propolis; Phenolic compounds/determination in propolis; Propolis/quality control.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300023

Anticonvulsant profile of 2-ethylthio-7-methyl-4-(4-methylphenyl)pyrazolo[1,5-a][1,3,5]triazine
Provedor de dados: 60
Autores: Estrada,Martín Hermógenes; Insuasty,Henry; Cuca,Luis Enrique; Marder,Mariel; Fierro,Angélica; Guerrero,Mario Francisco.
This work evaluates the central nervous effects in ICR strain mice of 2-ethylthio-7-methyl-4-(4-methylphenyl)pyrazolo[1,5-a][1,3,5]triazine (MH4b1), a compound obtained by an efficient one-step reaction of S,S-diethyl 4-methylbenzoylimidodithiocarbonate with 5-amino-3-methyl-1H-pyrazole, in order to assess its neuro-pharmacological profile. The tests applied were: maximal electroshock seizure (MES), pentylenetetrazole (PTZ) seizures, forced swimming, plus maze, marble burying, sleeping time, rota-rod and catalepsy. In addition, MH4b1 binding to the benzodiazepine site of the GABA-A receptor and MH4b1 inhibition of monoamine oxidase (MAO) subtypes A and B were evaluated. MH4b1 showed anticonvulsant effects in a dose dependent manner (30-300 mg/kg, p.o.)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrazolo-triazine/anticonvulsant profile; Anticonvulsants/experimental study; Monoamine oxidase/inhibitors.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100007

Association between kidney function and Framingham risk score in an admixed population of Brazil
Provedor de dados: 60
Autores: Costa,Brunna Soares Rodrigues; Freitas,Erika Paula Silva; Cruz,Marina Sampaio; Duarte,Victor Hugo Rezende; Silva,Ananília Medeiros Gomes da; Santos,Isabelle Cristina Clemente dos; Santos,Jéssica Cavalcante dos; Rezende,Adriana Augusto; Sena-Evangelista,Karine Cavalcanti Maurício; Silbiger,Vivian Nogueira.
ABSTRACT Chronic kidney disease (CKD) increases cardiovascular disease (CVD) risk development. However, the mechanisms of reduced kidney function with CVD risk are unclear. This study aimed to investigate the association between kidney function and Framingham risk score (FRS) in participants with traditional cardiovascular risk factors and normal estimated glomerular filtration rate (eGFR) &gt; 60 mL/min/1.73 m² in an admixed population of Brazil. The participants were divided into three groups according to FRS: low risk group with 0% to &lt;10%, moderate risk group with ≥10% to 20% and high risk group with &gt;20%. The eGFR was calculated using Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI). Data from participants were collected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney disease; Cardiovascular diseases/risk factors; Glomerular filtration rate; Framingham risk score (FRS).
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300626

Toxicological and pharmacological evaluation of Discaria americana Gillies &amp;amp; Hook (Rhamnaceae) in mice
Provedor de dados: 60
Autores: Silva,Eliane Regina da; Diedrich,Denise; Bolzan,Rodrigo Cordeiro; Giacomelli,Sandro Rogério.
Medicinal plants (e.g. Discaria americana) have been used by populations for centuries. However, popular use is not enough to validate these plants as safe and effective medicinal products. The present study sought to evaluate the acute and subacute toxicity as well as the anxiolytic and antinociceptive effects of D. americana root bark and aerial parts extracts in mice. In acute toxicity studies, mice were treated with single intraperitoneal doses of the aforementioned extracts. Subacute toxicity studies were performed by oral administration of the extracts over 14 days. Anxiolytic studies consisted of the elevated plus maze method, and antinociceptive studies were based on the hot plate test. The LD50 value for D. americana aerial parts extract was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Discaria americana/phamacognosy; Medicinal plants; Discaria americana/toxicological analysis; Discaria americana/acute toxicity; Discaria americana/subacute toxicity; Discaria americana/anxiolytic effect; Discaria americana/antinociceptive effect.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200011

Analysis of chlorpheniramine in human urine samples using dispersive liquid-liquid microextraction combined with high-performance liquid chromatography
Provedor de dados: 60
Autores: Maham,Mehdi; Kiarostami,Vahid; Waqif-Husain,Syed; Sharifabadi,Malihe Khoeini.
A simple and environmentally friendly microextraction technique was used for determination of chlorpheniramine (CPM), an antihistamine drug, in human urine samples using dispersive liquid-liquid microextraction (DLLME) followed by high performance liquid chromatography with diode array detection (HPLC-DAD). In this extraction technique, an appropriate mixture of acetonitrile (disperser solvent) and carbon tetrachloride (extraction solvent) was rapidly injected into the urine sample containing the target analyte. Tiny droplets of extractant were formed and dispersed into the sample solution and then sedimented at the bottom of the conical test tube by centrifugation. Under optimal conditions, the calibration curve was linear in the range of 0.055-5.5 µg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorpheniramine/determination in human urine; High-performance liquid chromatography/quantitative analysis; Dispersive liquid-liquid microextraction/quantitative analyisis; Antihistamines; Human urine/analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300551

The challenge of responsible dispensing: formal education versus professional practice
Provedor de dados: 60
Autores: Bezzegh,Nadine Judith; Goldenberg,Paulete.
The aim of the present study was to evaluate the education of Pharmaceutical Technicians for the activity of responsible dispensing. Based on a questionnaire with open and closed questions, the study sought to characterize the students, identify knowledge and attitudes regarding the Rational Use of Medications while addressing the limits and possibilities of professional and ethical dispensing in practice. In addition, a group dynamics session - focus group - was held as a forum for debate on responsible dispensing. The results showed that students tended to be mature, currently employed and were predominately women. Displaying adequate knowledge on Rational Use of Medications and of the corresponding legislation, the students reported difficulties...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical technician/professional formation; Pharmaceutical technician/professional practice; Medicines/responsible dispensing; Medicines/rational use; Professional ethics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100008

E-learning in Pharmacy Education: what do we know about it?
Provedor de dados: 60
Autores: Lorenzoni,Andrigo Antonio; Manzini,Fernanda; Soares,Luciano; Leite,Silvana Nair.
The increase and flexibilization of e-learning in Brazil has generated concern about the low capacity to ensure the quality of institutions and courses. The objective is to identify the literature on experiences of pharmaceutical e-learning. A scope review was carried out, mapping key concepts and evidence. The selected articles, without period restriction, from the Scielo, Pubmed and Scopus databases provided data on the educational level of experience, modality, workload, content delivery method, instruments used, types of topics, evaluation information and purpose of the study. There were 87 articles on preparation for subsequent classes; feedback or continuation of previous classes; to specific training; the comparison between face-to-face and distance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacy education; E-learning; Distance learning.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100576

Determination of the critical hydrophile-lipophile balance of licuri oil from Syagrus coronata: application for topical emulsions and evaluation of its hydrating function
Provedor de dados: 60
Autores: Leal,Leila Bastos; Sousa,Giovana Damasceno; Seixas,Karoline Belém; Souza,Pedro Henrique Nogueira de; Santana,Davi Pereira de.
The aims of this study were to determine the critical hydrophile-lipophile balance (HLB) of licuri oil, and to perform a clinical assay to evaluate its hydrating effects. For the determination of the HLB, serial emulsions were prepared with the oil. Regarding the clinical study, 13 human subjects were recruited to evaluate the hydrating power of the emulsified preparation containing licuri oil, and comparing it with the same preparation containing sweet almond oil (SAO). The critical HLB of licuri oil was represented by the zones within the concentrations of 10% for the oil and 15% for the pair of tensoactive agents, with a value of 11.8. Both preparations showed similar hydrating power. We propose that licuri oil can be considered a new lipophilic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Licuri/fixed oil; Licuri/determination of hydrophile-lipophile balance; Licuri/fixed oil; Licuri/hydration effects; Emulsions/preparation with licuri oil; Skin hydration.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100018

Solid lipid nanoparticles of irbesartan: preparation, characterization, optimization and pharmacokinetic studies
Provedor de dados: 60
Autores: Soma,Deepthi; Attari,Zenab; Reddy,Meka Sreenivasa; Damodaram,Atmakuri; Koteshwara,Kunnatur Balasundara Gupta.
ABSTRACT Irbesartan is an antihypertensive with limited bioavailability and solid lipid nanoparticles (SLN) is one of the approaches to improve bioavailability. Solid lipid nanoparticles were prepared using glyceryl monostearate by solvent emulsification method followed by probe sonication. Irbesartan loaded SLNs were characterized and optimized by parameters like particle size, zeta potential, surface morphology entrapment efficiency and in vitro release. The optimized formulation was then further evaluated for the pharmacokinetic studies in Wistar rats. Irbesartan-loaded SLN of particle size 523.7 nm and 73.8% entrapment efficiency showed good bioavailability in Wistar rats and also showed optimum stability in the studies. The SLN prepared using glyceryl...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irbesartan/pharmacokinetics; Irbesartan/preparation; Irbesartan/bioavailability; Glyceryl monostearate; Solid lipid nanoparticles/characterization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100602

Spectrophotometric and HPLC determination of deflazacort in pharmaceutical dosage forms
Provedor de dados: 60
Autores: Scremin,Amarilis; Piazzon,Monika; Silva,Marcos Antonio Segatto; Kuminek,Gislaine; Correa,Giane Márcia; Paulino,Niraldo; Cardoso,Simone Gonçalves.
Deflazacort (DFZ) is a glucocorticoid used as an anti-inflammatory and immunosuppressant drug. No official methods are available for DFZ determination in pharmaceutical formulations. The objective of this study was to develop, validate and compare spectrophotometric (UV and colorimetric) and high-performance liquid chromatography (HPLC) methods, for the quantitative determination of DFZ in tablets and oral suspension. For the UV method, ethanol was used as the solvent, with detection at 244 nm. The colorimetric method was based on the redox reaction with blue tetrazolium in alkaline medium, with detection at 524 nm. The method by HPLC was carried out using a C18 column, mobile phase consisting of acetonitrile:water (80:20, v/v) with a flow rate of 1.0 mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deflazacort/quantitative determination; High performance liquid chromatography/quantitative analysis; Spectrophotometry/quantitative analysis; Medicines/quantitative analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200015

In vitro evaluation of Mucuna pruriens (L.) DC. antioxidant activity
Provedor de dados: 60
Autores: Longhi,Joy Ganem; Perez,Elisa; Lima,Jair José de; Cândido,Lys Mary Bileski.
Mucuna pruriens (L). Dc is a plant of the Fabaceae family, commonly known as velvet bean, itchy bean, chiporro bean, mucuna, among others. This plant has several medicinal properties, including its potential to treat Parkinson's disease (PD). International studies have shown that this plant surpasses the benefits of the substance levodopa in the treatment of PD. Taking into account that nerve cells are highly sensitive to oxidative substances, this study evaluated the antioxidant activity of mucuna and compared it to that of levodopa. The plant seeds' phenolic concentration was quantified by using the Folin-Denis reagent and the antioxidant activity assays were performed by using three different methods: the reduction of the phosphomolybdenium complex, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucuna pruriens; Antioxidant; Phenolic compounds.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300011

Analgesic and anti-inflammatory activities of several 4-substituted-6-(3'-nitrophenyl)pyridazin-(2H)-3-one derivatives
Provedor de dados: 60
Autores: Singh,Anita; Lakshmayya,; Asif,Mohammad.
Several 6-aryl-4-substituted benzylidene/furfurylidene pyridazin(2H)-3-one derivatives (4a-f) were synthesized and evaluated as analgesic and anti-inflammatory agents in mice and rats, respectively. All compounds were tested by using Eddy's hot plate and the carrageenan-induced hind paw oedema method for the evaluation of analgesic and anti-inflammatory activities, respectively. Results showed that compounds 4f, 4b, 4d, and 4e exhibited higher analgesic and anti-inflammatory activities than other remaining compounds. All title compounds (4a-f) were characterized by IR, NMR and Mass spectroscopy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyridazinone/evaluation; Pyridazinone/experimental study; Analgesics/evaluation; Analgesics/experimental study; Anti-inflammatories/evaluation; Anti-inflammatories/experimental study; Synthetic compounds/evaluation; Synthetic compounds/experimental study..
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400030

Metronidazole thermogel improves retention and decreases permeation through the skin
Provedor de dados: 60
Autores: Melo,Elayne Karine Souto de; Araujo,Thalita Pedon de; Silva,José Wellithom Viturino da; Chagas,Stephanye Carolyne Christino; Bedor,Danilo César Galindo; Santana,Davi Pereira de; Leal,Leila Bastos.
ABSTRACT Metronidazole (MTZ) is widely used as the standard antibiotic for the treatment of rosacea and, more recently, is being used off label in Brazilian hospitals for the treatment of wounds. Following oral administration, minimal amounts of active agent reaches the skin and side effects are strongly induced. Consequently, MTZ is currently being applied topically in order to improve the therapeutic efficacy with reduced side effects, with Rozex(r) (RZ) (an MTZ gelled formulation) being the only marketed product. This study examined whether the use of MTZ 0.75% from thermogel formulations could improve drug retention and reduce dermal exposure compared to that by Rozex(r). Following a 21 h permeation study, the highest total amount of MTZ permeated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metronidazole/evaluation; Metronidazole/drug retention; Poloxamer 407; Rosacea/treatment; Dermatopharmacokinetic.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200626

Validation of a method for simultaneous analysis of cocaine, benzoylecognine and cocaethylene in urine using gas chromatography-mass spectrometry
Provedor de dados: 60
Autores: Menotti,Vinicius Stela; Scanferla,Deborah Thais Palma; Oliveira,Kleber Ota de; Fratucci,Gabriela Felber; Alves,Gessé de Souza; Bando,Erika; Nerilo,Samuel Botião; Oliveira,Magda Lúcia Félix de; Machinski Junior,Miguel; Mossini,Simone Aparecida Galerani.
Abstract The present study describes a method for simultaneous analysis of cocaine, benzoylecgonine and cocaethylene in urine samples. After solid phase extraction, Gas Chromatography-Mass Spectrometry was used for identification and quantification. The calibration curves were linear at 20 - 3000 ng/mL, r2 0.9997 for benzoylecgonine, 15 - 2000 ng/mL, r2 0.9985 and r2 0.9993 for cocaine and cocaethylene, respectively. Accuracy values: cocaine: 93,5 - 102,1%; benzoylecgonine: 97,5 - 104,8%; cocaethylene: 90,6 - 101,5%. Precision values: cocaine: C.V 5,4 - 14,6%; benzoylecgonine: C.V 7,8 - 12,3%; cocaethylene: C.V 5,9 - 12,3%. Detection and quantification limit values: cocaine and cocaethylene:10 ng/mL and 15 ng/mL, respectivalty; benzoylecgonine:15 ng/mL and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Analytical validation; GC-MS; Cocaine; Human urine.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100627

Hydroethanolic  Allium sativum extract accelerates excision wound healing: evidence for roles of mast-cell infiltration and intracytoplasmic carbohydrate ratio
Provedor de dados: 60
Autores: Farahpour,Mohammad Reza; Hesaraki,Saeed; Faraji,Darab; Zeinalpour,Rasoul; Aghaei,Mohammad.
ABSTRACT The present study was designed to evaluate the in vivo effect of Allium sativum (garlic) hydroalcoholic extract on wound healing in rats. For this purpose, 72 mature Wistar rats were divided into four groups (n=18/each) to receive no treatment, placebo, Cicalfate(r), or 2% Allium sativum (AS) extract, administered topically to the wound area, for 21 days. Following the experimental period, tissue samples were dissected out and underwent to histopathological analyses. Fibroblasts, fibrocytes, mast cells, intra-cytoplasmic carbohydrate ratio, neovascularization, collagen deposition, and re-epithelialization were analyzed in all groups. Animals in the treated groups showed significant enhancement in fibroblast, fibrocyte, and mast-cell distribution....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium sativum/effects/rats; Allium sativum/wound healing/study; Allium sativum/mast cells; Allium sativum/angiogenesis; Intra-cytoplasmic carbohydrate.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100605

Pharmacokinetics of diltiazem hydrochloride delay-onset sustained-release pellet capsules in healthy volunteers
Provedor de dados: 60
Autores: Yan,Xi-Qing; Chen,Zhi-Gang; Wang,Rong-Liang; Yang,Jun; Ai,Fang; Pan,Yan-Juan; Qiu,Pei-Yong; Wang,Da-Xin.
The pharmacokinetics (PK) of ordinary tablets and sustained release capsules of diltiazem hydrochloride in human clinical trials had been studied. The PK of diltiazem hydrochloride delay-onset sustained-release pellet capsules, a new dosage form, has not been reported, although it is very important to clinical use. In this paper, we investigated the PK of diltiazem hydrochloride delay-onset sustained-release pellet capsules and the food influence in Chinese healthy volunteers. The PK parameters indicated that the diltiazem hydrochloride delay-onset sustained-release pellet capsules appeared marked characteristics of delayed and controlled release. An opened-label, randomized and parallel clinical trial was conducted in 36 Chinese healthy volunteers with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diltiazem hydrochloride/delay-onset sustained-release pellet capsules; Diltiazem hydrochloride/pharmacokinetics; Food/ingestion; Food/effect in pharmacokinetic profile; High-performance liquid chromatography/quantitative analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100004

Synthesis of leading chalcones with high antiparasitic, against Hymenolepis nana, and antioxidant activities
Provedor de dados: 60
Autores: Díaz-Carrillo,José Tomás; Díaz-Camacho,Sylvia Páz; Delgado-Vargas,Francisco; Rivero,Ignacio Alfredo; López-Angulo,Gabriela; Sarmiento-Sánchez,Juan Ignacio; Montes-Avila,Julio.
The hymenolepiosis by Hymenolepis nana is a major public health problem in developing countries, and the commercial drugs against this parasitosis are not enough effective. The combination of antiparasitic and antioxidant agents has improved the treatment of some parasitoses. Thus, the development of new cestocidal and antioxidant agents to treat the hymenolepiosis cases is important. In the present study, four hydroxy- and four dihydroxy-chalcones were synthesized using the catalyst boron trifluoride diethyl etherate (BF3•OEt2). The antioxidant activity and antiparasitic against H. nana of chalcones were tested, as well as the toxicity by the brine shrimp lethality bioassay and the method of Lorke. The antioxidant activity was measured by three radical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenolepis nana; Chalcone/synthesis/antiparasitic/antioxidant activity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300610

Radiolabeling of cidofovir with technetium-99m and biodistribution studies
Provedor de dados: 60
Autores: Arribada,Raquel Gregorio; Pereira,Nara Caroline; Cardoso,Valbert Nascimento; Cunha Júnior,Armando da Silva; Barros,André Luís Branco de.
Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cidofovir; Radiotracer; Technetium-99m; Biodistribution; Scintigraphic imaging.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100570

Composition of essential oil and allelopathic activity of aromatic water of Aster lanceolatus Willd: (Asteraceae)
Provedor de dados: 60
Autores: Dias,Josiane de Fátima Gaspari; Miguel,Obdúlio Gomes; Miguel,Marilis Dallarmi.
The essential oil obtained from flowers of Aster lanceolatus was submitted the CG-MS and presented as result thirteen substances with largest concentration; among them, the caryophyllene oxide with the larger one. The aromatic water obtained during the extraction process of this essential oil was forwarded to allelopathic test, and demonstrated to be capable to inhibit the germination and growth of Lactuca sativa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oil; Aster lanceolatus; Caryuphyllene oxide; Aromatic water; Allelopathy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300012

Physical methods for topical skin drug delivery: concepts and applications
Provedor de dados: 60
Autores: Petrilli,Raquel; Lopez,Renata Fonseca Vianna.
Topical drug delivery is an interesting approach to treat skin diseases and to avoid pain and low patient compliance in cases where a systemic delivery is required. However, the stratum corneum, which is the outermost skin layer, strongly protects the body from the entrance of substances, especially those hydrophilic. In this context, different physical methods have been studied to overcome the stratum corneum barrier and facilitate penetration of drugs into or through the skin. Among them, iontophoresis, low-frequency ultrasound and microneedles have been widely employed for transdermal drug delivery. More recently, they are also studied to aid in the treatment of dermatological disorders, such as skin tumors and inflammation. Basically, iontophoresis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iontophoresis; Sonophoresis; Microneedles; Skin delivery.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700407

A sensitive bioanalytical method development and validation of cabozantinib in human plasma by LC-ESI-MS/MS
Provedor de dados: 60
Autores: Inturi,Srikanth; Avula,Prameela Rani.
ABSTRACT A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of cabozantinib (CZ) in human plasma using cabozantinib-d4 (CZD4) as an internal standard (IS). Chromatographic separation was performed on Xbridge C18, 50 x 4.6 mm, 5 mm column with an isocratic mobile phase composed of 10mM Ammonium formate and Methanol in the ratio of (20:80 v/v), at a flow-rate of 0.7 mL/min. CZ and CZD4 were detected with proton adducts at m/z 502.2 ® 391.1 and 506.3 ® 391.2 in multiple reaction monitoring (MRM) positive mode respectively. Liquid-Liquid extraction method was used to extract the drug and IS. The method was validated over a linear concentration range of 5.0-5000.0 pg/mL with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cabozantinib/human plasma/validation; Mass spectrometry; Pharmacokinetic/study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200622

Celebrating the 120th anniversary of the School of Pharmaceutical Sciences of the University of São Paulo
Provedor de dados: 60
Autores: Borelli,Primavera.
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700100

Development and validation of a simple spectrophotometric method for the determination of methyldopa in both bulk and marketed dosage formulations
Provedor de dados: 60
Autores: Ribeiro,Paulo Roberto da Silva; Duarte,Reginária Morais.
A simple, precise, sensitive, rapid, specific and economical spectrophotometric method was developed to determine methyldopa (MTD) content in bulk and pharmaceutical dosage formulations. The proposed method was based on the formation of a colored product from the nitrosation reaction of MTD with sodium nitrite in an acid medium. The resultant nitroso derivative species reacts further with sodium hydroxide and is converted it into a more stable compound. This yellow nitrosation product exhibited an absorption maximum at 430 nm. Beer's Law was obeyed in a concentration range of 6.37 to 82.81 μg mL-1 MTD with an excellent coefficient of determination (R2 = 0.9998). No interference was observed from common excipients in formulations. The results showed the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methyldopa/spectrophotometric determination; Pharmaceutical formulations/quality control; Spectrophotometry/qualitative analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300573

Responsible self-medication: review of the process of pharmaceutical attendance
Provedor de dados: 60
Autores: Galato,Dayani; Galafassi,Luciana de Mattos; Alano,Graziela Modolon; Trauthman,Silvana Cristina.
This article presents a review, based on a qualitative study, of pharmaceutical orientation in the management of minor illness. Action research methodology was used by a group of faculty members responsible for the community pharmacy internship and by postgraduates in clinical pharmacy, to carry out the study with the objective to present a standard service for this kind of procedure. The interaction with the individual starts with a welcoming reception, at which point the pharmacist should be receptive and show empathy. Subsequently, data from the history of the patient are collected to obtain relevant information. Based on this information, the pharmacist must develop a line of clinical reasoning and make a decision, taking the context of the patient...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Self-medication; Community pharmacy; Drugs/rational use; Pharmaceutical orientation; Responsible self-medication; Minor illness.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400004

Potential drug delivery system: study of the association of a model nitroimidazole drug with aggregates of amphiphilic polymers on aqueous solution
Provedor de dados: 60
Autores: Salamanca Mejia,Constain Hugo; Urbano,Bruno Felipe; Olea Carrasco,Andres Floreal.
This study evaluated the association of N-hexyl-2-methyl-4-nitroimidazol, a model drug, to aggregates formed by anionic polyelectrolytes on aqueous solution. The alternating copolymers of maleic anhydride and N-vinyl-2-pyrrolidone were synthesized and then modified by reaction of the anhydride groups with aliphatic amines and alcohols of varying length of the alkyl chain. The partition of the model drug between water and the hydrophobic microdomains provided by the copolymers was studied using the pseudo-phase model to determinate the distribution coefficient K S, and the standard free energy of transfer ∆µ°t. The results indicate that all copolymers assessed are potential pharmaceutical reservoirs of the model drug. Nevertheless, the solubility of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitroimidazole; N-vinil-2-pyrrolidone; Copolymers/evaluation; Maleic acid; N-hexil-2-methyl-4-nitroimidazol; Drugs reservatories.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400009

Determination of apramycin in oral soluble powder by a HPLC method using pre-column derivatization with o-phthalaldehyde and UV detection
Provedor de dados: 60
Autores: Antunes,Elisabete de Almeida Barbosa; Lourenço,Felipe Rebello; Pinto,Terezinha de Jesus Andreoli.
A high-performance liquid chromatographic method employing pre-column derivatization with o-phthalaldehyde (OPA) and 2-mercaptoacetic acid was developed for the determination of apramycin, an aminoglycoside antibiotic used in veterinary medicine, in the oral soluble powder form. The chromatographic separation was done by ion-pair HPLC using a C18 reversed-phase column, Synergy Hydro (150 mm x 4.6 mm x 4 µm) and mobile phase composed of 0.005 mol/L sodium octanosulfonate in a mixture of acetonitrile: water: acetic acid (45:55:2) (v/v/v) with a flow rate of 1.0 mL/min; the UV detector was operated at 332 nm. The developed method was validated according to official compendia guidelines, having demonstrated robustness, selectivity and linearity for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apramycin; HPLC; Microbiological Assay; Pre-column Derivatization; OPA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200007

Formulation and evaluation of taste mask pellets of granisetron hydrochloride as oro dispersible tablet
Provedor de dados: 60
Autores: Choudhary,Nilesh; Avari,Jasmine.
Orally disintegrating systems have carved a niche amongst the oral drug delivery systems due to the highest compliance of the patients, especially the geriatrics and pediatrics. In addition, patients suffering from dysphagia, motion sickness, repeated emesis and mental disorders prefer these medications because they cannot swallow large quantity of water. Further, drugs exhibiting satisfactory absorption from the oral mucosa or intended for immediate pharmacological action can be advantageously formulated in these dosage forms. However, the requirements of formulating these dosage forms with mechanical strength sufficient to withstand the rigors of handling and capable of disintegrating within a few seconds on contact with saliva are inextricable. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Granisetron hydrochloride/taste masking; Oro Dispersible Tablet (ODT); Pellets/coating; Oral drugs/delivery system.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300569

Effects of long-term fluoxetine treatment on adrenergic plasticity in rat vas deferens
Provedor de dados: 60
Autores: Souza,Cláudio Fontes; Garcez-do-Carmo,Lúcia; Conceição,Isaltino Marcelo; Freitas,Thalma Ariani; Frussa-Filho,Roberto.
Chronic antidepressant administration increases neurotrophin levels in the central and peripheral nervous system, leading to an increase of neuronal sprouting, reestablishment of neural networks and neurotransmitter levels. Injured peripheral nerves regenerate at very slow rates. However, the recovery of the hypogastric nerve in rodents after injury is significantly improved with neurotrophin administration. Accordingly, our goal was to determine whether treatment with the antidepressant fluoxetine affects catecholamine levels and neuronal function, after surgical denervation of the rat vas deferens. Noradrenaline levels in the denervated vas deferens were higher in fluoxetine-treated animals than in the vehicle-treated group, as measured by high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoxetine/chronic administration/effects; Denervation; Antidepressants/long term use/experimental study; Adrenergic neurotransmission; Noradrenaline/neuronal release.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400757

Development of a generic method for the determination of proton-pump inhibitors by capillary zoneelectrophoresis
Provedor de dados: 60
Autores: Papp,Lajos Attila; Gyéresi,Árpád; Hancu,Gabriel; Mircia,Eleonora; Kelemen,Hajnal.
A generic capillary zone electrophoresis method was developed for the analysis of four proton pump inhibitors: omeprazole, pantoprazole, lansoprazole and rabeprazole. During preliminary analysis screening of phosphate buffers at different pH levels was performed, in order to determine the optimum pH domain suitable for the simultaneous determination of all studied compounds. A face centered central composite design was employed for the optimization of separation conditions. The effect of buffer concentration, pH and applied voltage was studied; resolution between peaks and migration time of the last compound were considered as responses. Other factors as system temperature, injection parameters, capillary length, were held constant during the optimization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capillary electrophoresis; Proton-pump inhibitors; Experimental design.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100534

Influence of high-dose gamma radiation and particle size on antioxidant properties of Maize ( Zea mays L.) flour
Provedor de dados: 60
Autores: Nawaz,Haq; Shad,Muhammad Aslam; Rehman,Tanzila; Ramzan,Ayesha.
ABSTRACT Influence of high-dose gamma radiation and particle size on antioxidant properties of maize (Zea mays L.) flour was studied using response surface methodology. A central composite design based on three levels of each of particle size, in terms of mesh number (40, 60 and 80 meshes), and gamma radiation dose (25, 50 and 75 kGy) was constructed. A statistically significant dose-dependent decrease (p&lt;0.05) in antioxidant properties of gamma irradiated flour was observed. However, an increase in the mesh number (decrease in particle size of flour) resulted in an increase in antioxidant properties. The optimum level of radiation dose to achieve maximum value of responses was found to be 50 kGy for Trolox equivalent total antioxidant activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize flour/antioxidant properties; Maize flour/gamma irradiation/effects; Central composite design; Maize flour/particle size; Response surface methodology; Zea mays L.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400771

Protein PEGylation for the design of biobetters: from reaction to purification processes
Provedor de dados: 60
Autores: Santos,João Henrique Picado Madalena; Torres-Obreque,Karin Mariana; Meneguetti,Giovanna Pastore; Amaro,Beatriz Panichi; Rangel-Yagui,Carlota Oliveira.
The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes of the protein, reduce degradation by proteolytic enzymes, enhance long-term stability and maintain or even improve pharmacokinetic and pharmacodynamics characteristics of the protein drug. Nonetheless, correct information in terms of the PEGylation process from reaction to downstream processing is of paramount importance for the industrial application and processing scale-up. In this review we present and discuss the main steps in protein PEGylation, namely: PEGylation reaction, separation of the products and final characterization of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PEGylation; Biobetters; Biological drugs; Polyethylene glycol; Protein purification; Site-specific PEGylation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700408

Microwave-assisted synthesis and pharmacological screening of some triazolothiadiazole derivatives
Provedor de dados: 60
Autores: Gorgu,Ozge; Yıldırım,Engin; Ozkan,Yesim; Cakır,Bilge; Erol,Kevser; Onkol,Tijen.
In this study, twenty-two new [1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles (5a-n, 6a-h) were synthesized under microwave irradiation (MWI). The chemical structures of the compounds were elucidated by their IR, 1H-NMR, LC-MS, and elemental analysis. The compounds were tested for antinociceptive activity by using the tail clip, tail flick, hot plate, and writhing methods in mice. The varying levels of antinociceptive activity of the compounds were compared with those of aspirin. Among these compounds, compound 5g and 5j were found to be significantly more active than the other compounds and the standard in the tests. Also, inhibitory effects of the test compounds on COX-1 and COX-2 activities were investigated. DuP-697 for COX-2 and SC-560 for COX-1 were used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2(3H)-Benzoxazolone; Triazolothiadiazole; Antinociceptive activity; Microwave-assisted synthesis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100526

Development of a mouse computational model for MCNPx based on Digimouse (r) images and dosimetric assays
Provedor de dados: 60
Autores: Mendes,Bruno Melo; Almeida,Iassudara Garcia de; Trindade,Bruno Machado; Fonseca,Telma Cristina Ferreira; Campos,Tarcísio Passos Ribeiro de.
ABSTRACT The aim of this study was to create and test a new mice 3D-voxel phantom named DM_BRA for mice and human first-estimation radiopharmaceutical dosimetry. Previously, the article reviews the state-of-art in animal model development. Images from Digimouse CT database were used in the segmentation and on the generation of the voxelized phantom. Simulations for validation of the DM_BRA model was performed at 0.015, 0.1, 0.5, 1 and 4 MeV photons with heart-source. Specific Absorbed Fractions (SAF) data were compared with literature data. The organ masses of DM_BRA correlated well with existing models based on the same dataset; however, few small organ masses hold significant variations. The SAF data in most simulated cases were statistically equal to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dosimetry/assay; Mouse phantom/tests; MCNPx Monte Carlo code Computational studies.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100622

Short-term oral administration of risperidone induces pancreatic damage and hyperamylasemia in Sprague-dawley rats
Provedor de dados: 60
Autores: Shah,Rehmat; Subhan,Fazal; Sultan,Syed Muhammad; Ali,Gowhar.
Risperidone is an atypical antipsychotic acting mainly as a dopamine D2 and serotonin 5-HT2 receptors antagonist prescribed in the treatment of schizophrenia and various affective disorders. Risperidone has been reported to be associated with weight gain, panreatitis and type 2 diabetes mellitus. Various mechanisms of risperidone-induced toxicities have been reported but the histology of tissues especially pancreas has never been studied. Therefore, the current study was designed to elucidate the toxic effects of chronic administration of risperidone on pancreas, liver and kidneys. Animals (rats) of either gender were divided into two groups, the risperidone and control groups. Risperidone was administered in a dose of 2.5 mg/kg/d for three weeks. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Risperidone/antipsychotics/oral administration/effects; Histopathology; D2 antagonist; Pancreas.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400629

Topical ophthalmic antimicrobials: unfulfilled demands and possibility of new investments in Brazil and in the United States
Provedor de dados: 60
Autores: Kollross,Bianca; Gelfuso,Guilherme Martins; Cunha-Filho,Marcilio; Gratieri,Tais.
In general, topical ophthalmic drug products, especially those used for treating infections, present low effectiveness because of various reasons, from unfavorable drug physicochemical properties to physiological protective mechanisms of the eye. The fact is such group of products holds room for improvement, which could mean the development of better drugs or dosage forms. To achieve this, the knowledge of market composition is essential. The present work studied and compared the antimicrobial ophthalmic markets of Brazil and of the United States (US). Official databank of Brazilian Health Regulatory Agency and of US Food and Drug Administration were assessed for registered antimicrobial topical ophthalmic drug products. Brazilian market has registered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ophthalmologic drug products; Registration/Brazil; Registration/United States.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100573

L-arginine, a nitric oxide precursor, reduces dapsone-induced methemoglobinemia in rats
Provedor de dados: 60
Autores: Moraes,Natália Valadares de; Bergamaschi,Mateus Machado; Bianchi,Maria de Lourdes Pires; Bragheto,Juliana Bordinassi; Malfará,Wilson Roberto; Queiroz,Regina Helena Costa.
Dapsone use is frequently associated to hematological side effects such as methemoglobinemia and hemolytic anemia, which are related to N-hydroxylation mediated by the P450 enzyme system. The aim of the present study was to evaluate the influence of L-arginine supplementation, a precursor for the synthesis of nitric oxide, as single or multiple dose regimens on dapsone-induced methemoglobinemia. Male Wistar rats were treated with L-arginine at 5, 15, 30, 60 and 180 mg/kg doses (p.o., gavage) in single or multiple dose regimens 2 hours prior to dapsone administration (40 mg/kg, i.p.). The effect of the nitric oxide synthase inhibitor L-NAME was investigated by treatment with multiple doses of 30 mg/kg (p.o., gavage) 2 hours before dapsone administration....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dapsone; Methemoglobinemia; L-arginine; Nitric oxide; L-NAME.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100010

Synthesis and MALDI-ToF characterization of dendronized poly(ethylene glycol)s
Provedor de dados: 60
Autores: Myers,Brittany K.; Lapucha,Joanna E.; Grayson,Scott M.
Well-defined hybrids of linear poly(ethylene glycol)s (PEGs) and dendritic polyesters were prepared via the dendronization of the alcohol end groups of the mono and difunctional linear PEGs. Though useful for rudimentary product characterization, GPC and NMR could not verify the overall structural purity of these linear-dendritic hybrids. On the other hand, the detailed data provided by MALDI-ToF mass spectrometry enabled confirmation of the high structural purity of the dendronized PEGs at each step of the dendronization procedure. The well-defined number of functionalities on these dendronized PEGs, renders them particularly useful for research in the biomedical sphere where functionality and purity are of the utmost importance. The MALDI-ToF mass...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poly(ethylene glycol); Dendrons; Dendrimers; Dendronized polymers.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700005

Protective effects of salidroside on chronic heart failure in rats and the underlying mechanisms
Provedor de dados: 60
Autores: Zhang,Chengxi; Pan,Sinian; Tang,Leile; Ling,Yesheng; Zhou,Xiaojing; Feng,Wei.
The present study aimed to investigate the protective effects of salidroside on chronic heart failure (CHF) in rats and to explore the underlying mechanisms. One hundred SD rats were randomly divided into sham-operated, model, and low-, medium- and high-dose salidroside groups. The CHF model was established in later 4 groups. The later 3 groups were intragastrically administrated with 6, 12 and 24 mg/kg salidroside, respectively, once a day, for continuous 4 weeks. Finally, the serum levels of brain natriuretic peptide (BNP) and interleukin 6 (IL-6), cardiac function indexes, and expression levels of myocardial cysteinyl aspartate-specific proteinase (Caspase)-3, Caspase-9, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salidroside/protective effects; Chronic heart failure.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100517

Evaluation of the impact of orally administered carbohydrates on postprandial blood glucose levels in different pre-clinical models
Provedor de dados: 60
Autores: Marques,Any de Castro Ruiz; Schiavon,Fabiana Percinoto Monteiro; Travassos,Patricia Batista; Eik,Vanessa Fontana; Godoy,Guilherme; Schamber,Christiano Rodrigues; Bazotte,Roberto Barbosa.
ABSTRACT We developed a pre-clinical model in which to evaluate the impact of orally administered carbohydrates on postprandial blood glucose levels. For this purpose, we compared the effects of different carbohydrates with well-established glycemic indexes. We orally administered (gavage) increasing amounts (0.2, 0.4, 0.6, 0.8, and 1.0 g/kg) of sucrose and lactose to rats which had been fasted for 6 h or 15 h, respectively. In part of the experiments we administered frutose (gavagem). Three different models were compared for measuring postprandial blood glucose levels: a) evaluation of interstitial glucose concentrations by using a real time continuous glucose monitoring system; b) evaluation of glucose levels in blood obtained from the rat tail; c)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycemic index/study; Glycemic index/oral administration; Glycemic load; Interstitial glucose; Real time continuous glucose monitoring system; Carbohydrates/posprandial glucose levels/rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400761

Perspectives and challenges in pharmaceutical care
Provedor de dados: 60
Autores: Storpirtis,Sílvia.
Tipo: Info:eu-repo/semantics/article
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400001

Stability study of azithromycin in ophthalmic preparations
Provedor de dados: 60
Autores: Moreno,Andréia de Haro; Silva,Maria Fernanda Conz da; Salgado,Hérida Regina Nunes.
A stability study of azithromycin in ophthalmic preparations was developed by submission to different types of light, temperature and pH, using the biodiffusion assay (cylinder 3 x 3) for the quantifications. Bacillus subtilis, ATCC 9372, was used as test organism. The used concentration range was of 50 to 200 µg/mL. The study demonstrated that the drug suffered degradation when submitted to the ultraviolet light, germicide light, solar luminosity, acid solution, basic solution and hydrogen peroxide solution. The results were analyzed by the analysis of variance (ANOVA).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azithromycin/stability study; Bioassay; Photodegradation; Ophthalmic preparations.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200005

Efficacy of Origanum essential oils for inhibition of potentially pathogenic fungi
Provedor de dados: 60
Autores: Souza,Nadábia Almeida B; Lima,Edeltrudes de Oliveira; Guedes,Diego Nunes; Pereira,Fillipe de Oliveira; Souza,Evandro Leite de; Sousa,Frederico Barbosa de.
This study aimed to assess the efficacy of O. vulgare L. and O. majorana L. essential oil in inhibiting the growth and survival of potentially pathogenic fungal strains and also sought to evaluate the possible mechanisms involved in the establishment of the antifungal property of the tested essential oils through assays of osmotic stability and morphogenesis. Test strains included in this study were Candida albicans ATCC 7645, C. tropicalis LM-14, C. krusei LM-09, Cryptococcus neoformans FGF-5, Aspergillus flavus LM-02, A. fumigatus IPP-21, T. rubrum ATCC 28184, T. mentagrophytes LM-64, Microsporum gypseum ATCC 184, M. canis LM-36 and Cladosporium herbarium ATCC 26362. O. vulgare essential oil presented a MIC value of 80 µL/mL, while for O. majorana this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oil; Pathogen fungi; Origanum sp/antifungal property.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300013

Proposal of managerial standards for new product portfolio management in Brazilian pharmaceutical companies
Provedor de dados: 60
Autores: Moreira,Raquel Assis; Cheng,Lin Chih.
New Product Portfolio Management is aimed at helping decision-makers better select projects for new products based on key criteria for the manufacturer. The Brazilian pharmaceutical industry has been undergoing change due to stricter sanitary requirements following the enactment of the Generic Law in 1999. This paper presents the results of a research study aimed at clarifying the rationale employed by national pharmaceutical companies in selecting and prioritizing their new product development projects. Consequently, proposals for an analytical structure that could help these companies better select their products were produced. The research was carried out using case study methodology in which four different companies were investigated. The results of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Portfolio management; Pharmaceutical products/development; Pharmaceutical industry; Brazil/pharmaceutical industry.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100007

A new methodology for polyvalent intravenous immunoglobulin solution production with a two-stage process of viral inactivation
Provedor de dados: 60
Autores: Lucena,Antônio Edson de Souza; Sampaio,Divaldo de Almeida; Silva,Ednaldo Rosas da; Paiva,Virgínia Florêncio de; Santiago,Ana Cláudia; Leite,Ana Cristina Lima.
Highly purified intravenous immunoglobulin G concentrate (IV IgG) was produced with the use of polyethylene glycol associated to a single-stage precipitation by ethanol, instead of the classic Cohn-Oncley process, which employs cold alcohol as the precipitating agent, in a three-stage process. Precipitation of crude fraction containing more than 95% of immunoglobulin G was performed by liquid chromatography with a cation exchanger, CM-Sepharose, as a stationary phase. During the process, the product was subjected to two-stage viral inactivation. The first stage was performed by the action of sodium caprylate, 30 mM at pH 5.1+/- 0.1, and the second stage was performed by the action of a solvent-detergent mixture. The finished product was formulated at 5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood derivatives; Immunoglobulins; Polyethylene glycol; Ultrafiltration; Ion exchange chromatography.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400020

Mucoadhesive microemulsion of ibuprofen: design and evaluation for brain targeting efficiency through intranasal route
Provedor de dados: 60
Autores: Mandal,Surjyanarayan; Mandal,Snigdha Das; Chuttani,Krishna; Subudhi,Bharat Bhushan.
This study aimed at designing mucoadhesive microemulsion gel to enhance the brain uptake of Ibuprofen through intranasal route. Ibuprofen loaded mucoadhesive microemulsion (MMEI) was developed by incorporating polycarbophil as mucoadhesive polymer into Capmul MCM based optimal microemulsion (MEI) and was subjected to characterization, stability, mucoadhesion and naso-ciliotoxicity study. Brain uptake of ibuprofen via nasal route was studied by performing biodistribution study in Swiss albino rats. MEI was found to be transparent, stable and non ciliotoxic with 66.29 ± 4.15 nm, -20.9 ± 3.98 mV and 98.66 ± 1.01% as average globule size, zeta potential and drug content respectively. Transmission Electron Microscopy (TEM) study revealed the narrow globule size...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ibuprofen/brain-targeting; Ibuprofen/intranasal administration; Ibuprofen/mucoadhesion; Mucoadhesive microemulsion gel; Drugs/design; Transmission electron microscopy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300721

Development and evaluation of a hot-melt coating technique for enteric coating
Provedor de dados: 60
Autores: Patil,Arun Trambak; Khobragade,Deepak Shamrao; Chafle,Sandip Annaji; Ujjainkar,Amol Prasadrao; Umathe,Sudhir Niranjanrao; Lakhotia,Champalal Laxminarayan.
Conventional enteric coating requires the use of organic based polymers which are equally hazardous to the environment and operating personnel. Hot-melt coating avoids the use of solvents and is a safer and time-saving process. The present study was designed to assess the efficacy of hot-melt coating (HMC) as an enteric coating technique. Pellets prepared by extrusion spheronization were selected as the core formulation for a model of the gastric irritant drug diclofenac sodium (DFS) because of their innate advantages over single-unit formulations. Stearic acid (SA) and palmitic acid (PA) were evaluated as enteric hot-melt coating materials. HMC was carried out in a specially modified coating pan by applying SA and PA in molten state onto preheated pellets...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pellets/coating; Pellets/evaluation; Enteric coating/evaluation; Hot-melt coating/evaluation; Disintegration pH; Diclofenac sodium/pellets; Diclofenac sodium/coating; Palmitic acid/pellets; Palmitic acid/coating; Stearic acid/pellets; Stearic acid/coating.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100008

Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
Provedor de dados: 60
Autores: Bastos,Leandro Francisco Silva; Vago,Juliana Priscila; Caux,Thaís Rolla; Costa,Bruna Lopes; Godin,Adriana Martins; Menezes,Raquel Rezende; Pena,Roberta Ribas; Silva-Cunha,Armando; Sousa,Lirlândia Pires; Machado,Renes Resende; Moraes,Márcio Flávio Dutra; Fialho,Sílvia Ligório; Coelho,Márcio Matos.
Neuroimmune interactions underlying the development of pain sensitization in models of neuropathic pain have been widely studied. In this study, we evaluated the development of allodynia and its reduction associated with peripheral antineuroinflammatory effects induced by a dexamethasone-loaded biodegradable implant. Chronic constriction injury (CCI) of the sciatic nerve was performed in Wistar rats. The electronic von Frey test was applied to assess mechanical allodynia. The dexamethasone-loaded implant was placed perineurally at the moment of CCI or 12 days after surgery. Dorsal root ganglia (DRG; L4-L5) were harvested and nuclear extracts were assayed by Western blot for detection of nuclear factor (NF)-κB p65/RelA translocation. Dexamethasone delivered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic Constriction Injury; Dexamethasone/ pharmacology; Delivery device; Neuropathic Pain/ chemically induced; Nuclear Factor kappa B/ drug effects; Neuralgia/ chemically induced; NF-kappa B/ drug effects; Sciatic Nerve/ injuries; Absorbable Implants/ trends.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100591

Stir bar-sorptive extraction, solid phase extraction and liquid-liquid extraction for levetiracetam determination in human plasma: comparing recovery rates
Provedor de dados: 60
Autores: Freitas-Lima,Priscila; Ferreira,Flavia Isaura Santi; Bertucci,Carlo; Alexandre Júnior,Veriano; Dreossi,Sônia Aparecida Carvalho; Pereira,Leonardo Regis Leira; Sakamoto,Américo Ceiki; Queiroz,Regina Helena Costa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Levetiracetam/pharmacokinetic; High performance liquid chromatography; Antiepileptic drugs/therapeutic monitoring; Epilepsy/treatment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200393

Development and validation of a microbiological assay for determination of chlorhexidine digluconate in aqueous solution
Provedor de dados: 60
Autores: Fiorentino,Flávia Angélica Másquio; Corrêa,Marcos Antonio; Salgado,Hérida Regina Nunes.
Chlorhexidine (CHX) is a broad-spectrum antiseptic that is used in many topical pharmaceutical formulations. Because there is no official microbiological assay reported in the literature that is used to quantify CHX, this paper reports the development and validation of a simple, sensitive, accurate and reproducible agar diffusion method for the dosage of chlorhexidine digluconate (CHX-D) in an aqueous solution. The assay is based on the inhibitory effect of CHX-D upon the strain of Staphylococcus aureus ATCC 25923, which is used as the test microorganism. The design 3x3 parallel-line model was used. The results were treated statistically by analysis of variance (ANOVA), and they were excellent in terms of linearity (r = 0.9999), presenting a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agar diffusion; Antiseptic; Chlorhexidine; Microbiological assay; Quality control; Validation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200017

Pediatric medicines and their relationship to dental caries
Provedor de dados: 60
Autores: Menezes,Valdenice Aparecida de; Cavalcanti,Gabriela; Mora,Cristiana; Garcia,Ana Flávia Granville; Leal,Rossana Barbosa.
The aim of this study was to evaluate mothers' knowledge about the cariogenic potential of pediatric medicines. A total of 111 mothers were interviewed using a standardized form containing 15 questions relating to the association of pediatric drug use with dental caries and oral hygiene care. Descriptive and inferential statistics (Chi-square test and Fisher's exact) were used at a significance of 5%. Most of the mothers were aged 40 years or under (77.4%), high school educated (30.6%) and not working (50.5%). The association between medication use and dental caries or defects in teeth structure was mentioned by 35 (43.2%) mothers, 33 of whom (40.7%) cited this was due to the presence of sugar in the formulations. Only 32 mothers (28.8%) performed oral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pediatric medicine/cariogenic potential; Dental caries/prevention.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100018

Liposomes as potential carriers for ketorolac ophthalmic delivery: formulation and stability issues
Provedor de dados: 60
Autores: Mehanna,Mohammed Maher; El-Kader,Nouran Abd; Samaha,Magda Wadih.
ABSTRACT Drug delivery to treat ocular disorders locally is a challenging endeavor. Traditional ocular dosage form - eye drops - exhibits poor availability, consequently inefficient therapeutic response. The objective of the study was to formulate and characterize a ketorolac tromethamine ocular system with a prolonged release pattern based on liposomes as a vesicular carrier and to design once daily liquid preparation realizing the thermal in situ gelation principle. Liposomes were prepared by film hydration method. The influence of cholesterol concentration, pH and volume of hydration medium, and type and concentration of charging imparting agents were studied. Liposomes were characterized via, morphological examination, vesicular size, and encapsulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ketorolac/characterization/formulation; Liposomes/stability study; Ocular delivery; Thermosensitive gel; Prolonged release.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200618

Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS)
Provedor de dados: 60
Autores: Silveira,Rudinei da Silva; Leal,Gabriela Camera; Molin,Thais Ramos Dal; Faccin,Henrique; Gobo,Luciana Assis; Silveira,Géssica Domingos da; Souza,Mariana Tatsch da Silva; Lameira,Osmar Alves; Carvalho,Leandro Machado de; Viana,Carine.
This work describes the chemical characterization of extracts of Jatropha gossypiifolia (from Amazonia region) concerning the presence of phenolic and triterpenic compounds using Ultra High Perfomance Liquid chromatography coupled to Mass Spectrometry in Tandem (UHPLC-MS/MS) methods. The studied compounds belong to the most frequently found classes in medicinal plants (triterpenes, flavonoids, flavones, hydroxycinnamic acids, coumarins, catechins and stilbenes), which have been analyzed as chemical and bioactive markers in hydroethanolic and aqueous extracts. Several polyphenolic compounds identified herein are unprecedented in the scientific literature for this species. The chemical markers identified and quantified in the studied extracts of J....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jatropha gossypiifolia L; UHPLC-MS/MS; Phenolic compounds; Triterpenic compounds.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100539

Potentially inappropriate medication use in institutionalized older adults according to the Beers Criteria
Provedor de dados: 60
Autores: Ganassin,Amanda Rodrigues; Matos,Vanessa Terezinha Gubert de; Toffoli-Kadri,Mônica Cristina.
The need for specific care, coupled with new family arrangements, has contributed to the increasing institutionalization of elderly members. The purpose of this study was to evaluate drug use by institutionalized older adults according to Beers Criteria. This prospective, longitudinal study was conducted in the three non-profit long-stay geriatric care institutions of Campo Grande, in the Central-West region of Brazil. All subjects aged 60 years and above on November 2011 were included and followed until November 2012. Eighteen subjects were excluded and the final sample consisted of 133 individuals aged 60 to 113 years. Overall, 212 medications were used at geriatric care institution A, 532 at B, and 1329 at C. Thirty-four drugs were inappropriately...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/inappropriate use; Medicines/prescription; Polypharmacy; Elderly/inappropriate use of medicines; Beers Criteria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400827

Mushroom tyrosinase inhibitory activity and major fatty acid constituents of Amazonian native flora oils
Provedor de dados: 60
Autores: Teixeira,Raquel da Silva; Rocha,Paula Rafaela; Polonini,Hudson Caetano; Brandão,Marcos Antônio Fernandes; Chaves,Maria das Graças Afonso Miranda; Raposo,Nádia Rezende Barbosa.
In order to treat hyperpigmentation-related problems, there has been a global trend in developing cosmetics claiming to have skin-whitening properties, which act by inhibiting melanin biosynthesis. The objective of this work was to evaluate the in vitro mushroom tyrosinase inhibitory activity of five Amazonian native flora oils, and so to verify the possibility of their incorporation into cosmetic products. In addition, the fatty acid composition of the essential oils was determined by gas chromatography-flame ionisation detection in order to determine the main components of these oils. The tyrosinase inhibitory activity of the tested oils was found to be in the following order: açaí (IA50 = 66.08 µg mL-1) &gt; tucumã &gt; patauá &gt; pracaxi &gt; castanha...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian plants/evaluation; Amazonian plants/dermatological use; Skin lightening agents; Tyrosinase/inhibition; Tyrosinase/in vitro evaluation; Melanin; Hyperpigmentation/treatment.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300006

Bovine serum albumin-based nanoparticles containing the flavonoid rutin produced by nano spray drying
Provedor de dados: 60
Autores: Pedrozo,Regiellen Cristina; Antônio,Emilli; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.
Rutin is a flavonoid compound obtained from different vegetables and fruits; specifically, it is found in the seeds of buckwheat and in fruit peels, particularly citrus. It is also an important constituent of red wine. Rutin exhibits various biological properties including antiviral, vasoprotective, anti-inflammatory, and anticarcinogenic activities. However, its antioxidant activity is the most well studied. Despite the potential for in vitro applications, rutin presents low oral bioavailability that affects its biological activities. Nanoparticles composed of polymers, protein, or lipids are of great importance in the pharmaceutical and nutraceutical areas due to their physicochemical properties, which improve the pharmacokinetics of the drug which is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nano spray drying; Rutin; Nanoparticles; Bovine serum albumin; Antioxidant.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100512

Evaluation of community pharmacists’ involvement in public health activities in Nigeria
Provedor de dados: 60
Autores: Osemene,Kanayo Patrick; Erhun,Wilson Oyekigho.
The aims of the study were to identify the types, determine the extent of community pharmacists’ involvement, and evaluate the factors influencing their participation in public health activities in Nigeria. The study was a cross-sectional survey of randomly selected 130 registered community pharmacies. Pretested questionnaire was the instrument for data collection. Descriptive and inferential statistics were used to analyse data. Response rate was 94.9%. The study identified 31 types of public health activities which community pharmacists participated in. Their extent of involvement was highest in patient counseling (4.93 ± 0.25), personal hygiene (4.90 ± 0.37), maintenance of normal blood pressure (4.88 ± 0.32), and techniques for using vagina pessaries...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community pharmacists; Pharmacists; Counseling; Public Health; Nigeria.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300613

Spectroscopic and thermal characterization of alternative model biomembranes from shed skins of Bothrops jararaca and Spilotis pullatus
Provedor de dados: 60
Autores: Baby,André Rolim; Lacerda,Áurea Cristina Lemos; Sarruf,Fernanda Daud; Pinto,Claudinéia Aparecida Sales de Oliveira; Consiglieri,Vladi Olga; Serra,Cristina Helena dos Reis; Velasco,Maria Valéria Robles; Kawano,Yoshio; Kaneko,Telma Mary.
Recently, there has been an interest in the use of shed snake skin as alternative model biomembrane for human stratum corneum. This research work presented as objective the qualitative characterization of alternative model biomembranes from Bothrops jararaca and Spilotis pullatus by FT-Raman, PAS-FTIR and DSC. The employed biophysical techniques permitted the characterization of the biomembranes from shed snake skin of B. jararaca and S. pullatus by the identification of vibrational frequencies and endothermic transitions that are similar to those of the human stratum corneum.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomembrane/alternative models; Bothrops jararaca/skin; Bothrops jararaca/qualitative characterization; Spilotis pullatus/skin; Spilotis pullatus/qualitative characterization; Human stratum corneum/similars.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300006

Evaluation of polymeric PLGA nanoparticles conjugated to curcumin for use in aPDT
Provedor de dados: 60
Autores: Pietra,Renata Celi Carvalho de Souza; Cruz,Rosana Carvalho; Melo,Carla Nunes; Rodrigues,Lívia Bomfim; Santos,Patrícia Campi; Bretz,Gabriel Pissolati Matos; Soares,Betânia Maria; Sousa,Gerdal Roberto de; Ferreira,Marcus Vinícius Lucas; Cisalpino,Patrícia Silva; Magalhães,Paula Prazeres; Farias,Luiz de Macêdo; Pinotti,Marcos.
ABSTRACT Antimicrobial photodynamic therapy (aPDT) involves the association of a photosensitizing agent with a light source with the goal of causing apoptosis or microbial lysing. The use of compounds with natural active principles is gaining prominence throughout the world. Several studies from groups that are linked to the development of innovations in the pharmaceutical market have used natural dyes, such as curcumin, the efficacy of which has been demonstrated in aPDT trials. Difficulties related to physicochemical stability, solubility and cell penetration are some of the challenges associated with this field. The present work aimed to prepare, investigate the characteristics and improve the photodynamic activity of PLGA-based nanoparticles loaded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin/study; Nanoparticles/evaluation; PLGA; Antimicrobial photodynamic therapy (aPDT).
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200629

In Vitro additive effect on griseofulvin and terbinafine combinations against multidrug-resistant dermatophytes
Provedor de dados: 60
Autores: Lana,Aline Jacobi Dalla; Pippi,Bruna; Carvalho,Anderson Ramos; Moraes,Renata Cougo; Kaiser,Samuel; Ortega,George Gonzalez; Fuentefria,Alexandre Meneghello; Silveira,Gustavo Pozza.
ABSTRACT Griseofulvin (GF) and terbinafine (TF) are commonly used drugs to treat dermatophytosis, a fungal infection of the skin. Today there is an increase in drug resistance to these antifungals which highlight the need for alternative synergistic therapies. Minimum Inhibitory Concentration (MIC) of GF and TF were determined against fungi clinical isolates from local hospitals with values ranging 0.03-2.0 µg mL-1 and 0.24-4.0 µg mL-1, respectively. A checkboard test was used to determine the combination of GF:TF which could induce an additive effect against the fungi isolates Multidrug-resistant isolates showed susceptibility after treatment with 16:2 µg mL-1 GF:TF. An MTT assay further verified that GF and TF combinations have greater additive effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Griseofulvin; Terbinafine; Dermatophyte; Multidrug-resistant fungi; Drug combination.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200623

Anchusa italica extract: phytochemical and neuroprotective evaluation on global cerebral ischemia and reperfusion
Provedor de dados: 60
Autores: Torki,Akram; Khalaji-Pirbalouty,Valiallah; Lorigooini,Zahra; Rafieian-Kopaei,Mahmoud; Sadeghimanesh,Arezo; Rabiei,Zahra.
Abstract Stroke is the third leading cause of mortality and disability in industrial countries. Treatment with herbs with antioxidant properties has been reported to be an alternative to the conventional treatments. This study was conducted to investigate the effect of Anchusa italica extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in the rat. To do so, 50 rats were randomly assigned to five groups; control, sham, ischemia, and 50 or 100 mg/kg of Anchusa italica treated animals. Ischemia was induced by occlusion of carotid artery for 30 minutes. Afterward, behavioral tests and biochemical analyses were conducted. Induction of ischemia/reperfusion caused a decline in learning and passive avoidance memory in rats....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boraginaceae/phytochemistry; Brain ischemia; Spatial memory/drug effects; Malondialdehyde/pharmacology; Neuroprotection/drug effects; Lipidic peroxidation/drug effects; Antioxidant capacity; Passive avoidance memory.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100613

Development and validation of a stability-indicating LC-UV and LC-MS/MS methods for quantitative analysis of anisomycin and identification of degradation products
Provedor de dados: 60
Autores: Tolić,Ljiljana; Grujić,Svetlana; Laušević,Mila.
ABSTRACT Multifunctional drug anisomycin was subjected to forced degradation in accordance with International Conference on Harmonisation (ICH) guidelines for the first time. The drug was exposed to the recommended stress conditions of hydrolysis (acidic, alkaline and neutral), oxidation, thermal stress and photolysis, in order to investigate its stability. Optimized LC-MS/MS method was validated as recommended by ICH Q2(R1) guideline with respect to the specificity, accuracy, precision, limits of detection and quantitation, linearity and robustness. Anisomycin exhibited high instability under alkaline and thermal (neutral hydrolysis) conditions. It showed moderate stability under acidic, neutral, oxidative, thermal (acidic hydrolysis) and photolytic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anisomycin/forced degradation; Stability-indicating; LC-MS/MS/validation; Deacetylanisomycin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200607

Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studies
Provedor de dados: 60
Autores: Cazedey,Edith Cristina Laignier; Salgado,Hérida Regina Nunes.
New, simple and cost effective UV-spectrophotometric method was developed for the estimation of orbifloxacin in pharmaceutical formulation. Orbifloxacin was estimated at 290 nm in 0.5 M hydrochloric acid. Linearity range was found to be 1.0-6.0 μg mL-1. The method was tested and validated for various parameters according to main guidelines. The proposed method was successfully applied for the determination of orbifloxacin in tablets. The results demonstrated that the procedure is accurate, precise and reproducible, while being simple, economical and less time consuming. It can be suitably applied for the estimation of orbifloxacin in routine quality control and dissolution studies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/quality control; Medicines/dissolution; Orbifloxacin/determination; UV spectrophotometry/quantitative analysis/validation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300457

Improvement of fluconazole flowability and its effect on dissolution from tablets and capsules
Provedor de dados: 60
Autores: Consiglieri,Vladi Olga; Mourão,Samanta; Sampaio,Mauricio; Granizo,Patricia; Garcia,Pedro; Martinello,Valeska; Spricigo,Rodrigo; Ferraz,Humberto Gomes.
The aim of this work was to improve fluconazole flowability by wet granulation and to study the effect of granulation on drug dissolution from tablets and capsules. Fluconazole was submitted to a process of wet granulation in a high-speed granulator using Plasdone® K29/32 or K90. Flow properties of granules and dissolution profiles for tablets and capsules produced with them were determined. Fluconazole granules demonstrated better flowability, calculated by angle of repose and compressibility index data, compared with powder. Additionally, it was observed that the granulation process improved the dissolution efficiency (ED) of fluconazole from tablets and capsules, which could also suggest an increase in bioavailability. Higher dissolution efficiencies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluconazole/flowability; Tablets/dissolution; Capsules/dissolution; Plasdone.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100013

Piroxicam voltammetric determination by ultra low cost pencil graphite electrode
Provedor de dados: 60
Autores: Macêdo,Isaac Yves Lopes de; Alecrim,Morgana Fernandes; Oliveira Neto,Jerônimo Raimundo; Torres,Ieda Maria Sapateiro; Thomaz,Douglas Vieira; Gil,Eric de Souza.
Piroxicam (PRX) was determined in pharmaceutical capsules with differential pulse voltammetry (DPV) in a three electrode system consisting of a pencil graphite electrode (PGE) as working electrode, a Pt wire and a reference electrode of Ag/AgCl/KCl 3 M. An irreversible oxidation peak was observed in Epa c.a. 0.6 V, which correlates to the oxidation of PRX. The coefficient of linear correlation obtained was 0.9946, with limit of detection of 2.1 µM and limit of quantification of 4.7 µM. PGE assays showed good analytical performance compared to high performance liquid chromatography and spectrophotometry, showing the potential to be further developed and employed in quick and simple analyses.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electroanalysis; Pencil graphite electrode; Piroxicam; Voltammetry.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100504

Clinical outcomes of medication therapy management services in primary health care
Provedor de dados: 60
Autores: Mendonça,Simone de Araújo Medina; Melo,Angelita Cristine; Pereira,Gabriela Cristina Coelho; Santos,Danielle Maria de Souza Serio dos; Grossi,Elisa Brandão; Sousa,Maria do Carmo Vilas Boas; Oliveira,Djenane Ramalho de; Soares,Adriana Cristina.
ABSTRACT This study evaluates whether the integration of pharmacists into health-care teams through the delivery of pharmaceutical care-based medication therapy management (MTM) services can improve the clinical outcomes of patients with chronic health conditions in the primary health-care setting. A retrospective descriptive study of 92 outpatients assisted by MTM pharmacists in primary health-care units was carried out over 28 months (median follow-up: 05 months). Patients were followed up by MTM pharmacists, with a total of 359 encounters and a ratio of 3.9 encounters per patient. The prevalence of hypertension, diabetes mellitus and dyslipidaemia was 29.5%, 22.0% and 19.4%, respectively. There was a high prevalence of drug-related problems with a ratio...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care/primary care; Medication therapy management; Chronic health/conditions; Pharmacists/professional practice/Brazil.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300365

Ethnopharmacological survey: a selection strategy to identify medicinal plants for a local phytotherapy program
Provedor de dados: 60
Autores: Pereira,Flávia Liparini; Fernandes,José Martins; Leite,João Paulo Viana.
Ethnopharmacological studies are important for documenting and protecting cultural and traditional knowledge associated with the medical use of biodiversity. In this paper, we present a survey on medicinal plants used by locals in a community of Nova Viçosa, Viçosa, MG, Brazil, as a strategy to select medicinal plants for a phytotherapy-based local healthcare program. Eleven knowledgeable local informants were chosen by snowball sampling and interviewed about the use of medicinal plants. Plant samples were collected, herborised and then identified using traditional techniques and specialised literature. We sampled 107 medicinal plant species belonging to 86 genera and 39 families, predominantly Asteraceae with 16 species. Costus spicatus (Jacq.) Sw, M....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethnopharmacology; Medicinal plants; Phytotherapy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200014

Evaluation of adherence to pharmacological treatment
Provedor de dados: 60
Autores: Silva,William Henrique da; Dantas,Débora Santos; Nóbrega,Baldoíno Sonildo da; Queiroz,Maria do Socorro Ramos de; Alves,Harley da Silva.
The adherence to therapy associated with the socio-demographic variables and the habits of patients with hypertension and/or diabetes mellitus were investigated in this study. The registration forms of 105 patients in the Hiperdia program in the municipality of Campina Grande-PB were used as a data collection instrument, applying the Morisky-Green test (MGT) and Batalla test (BT) to assess compliance treatment. For the MGT, there was a prevalence of non-adherent individuals (76.2%) and the type of predominant behavior was unintentional. The internal consistency of the responses obtained through the MGT presented good reliability, according to the value of 0.69 obtained by Cronbach’s alpha. For the BT, there was a predominance of adherence (68.6%) and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Therapeutic adherence; Hypertension; Diabetes mellitus.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100572

Esculetin as bioactive marker: towards a rational scientific approach for the treatment of hyperuricemia using Traditional Chinese Medicine
Provedor de dados: 60
Autores: Zuo,Jieyu; Zhang,Wugang; Jian,Hui; Bou-Chacra,Nádia; Löbenberg,Raimar.
Traditional Chinese Herbal Medicine has been used to prevent and cure disease in China for thousands of years and has gained global interest in recent decades. The Erding formula is a Chinese Pharmacopeia (ChP)-listed herbal preparation used for treating sore throat, carbuncles and boils. Esculetin is a ChP quality control (QC) marker for these indications. A previous study found that a new indication, hyperuricemia, can be added to the Erding formula. Therefore, this study aimed to evaluate whether the traditionally used marker, esculetin, still has bioactivity for hyperuricemia, which is substantially different from the original indications. The study analyzed the quantity of esculetin by high-performance liquid chromatography, assessed the therapeutic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esculetin; Erding granules; Traditional Chinese Herbal Medicine; Bioactive marker.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100518

Pharmacist contributions for basic care from the perspective of professionals of familial health care teams
Provedor de dados: 60
Autores: Loch-Neckel,Gecioni; Crepaldi,Maria Aparecida.
This study aimed to investigate the social representations of professionals included in the team of Family Health Strategy (physicians, nurses and dentists) respecting the action possibilities and contributions of the pharmacist for the basic care, and based on social psychology and, particularly, on the theory of social representations. The epistemological basis of the research is qualitative, and the data were collected by means of individual semi-structured interviews, which were submitted to analysis of categorical thematic content. Apparently, the majority of professionals already inserted in the team know and recognize the importance of professional pharmacists in the basic care, as well as their potential contribution to this topic. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacist/action; Pharmaceutical intervention; Family Health; Pharmaceutical attention.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200011

Late cardiac perconditioning by phenylephrine in an isolated rat heart model is mediated by mitochondrial potassium channels
Provedor de dados: 60
Autores: Nazari,Afshin; Sedighi,Mehrnoosh; Dalvand,Parvin; Azizi,Yaser; Moghimian,Maryam; Boroujeni,Shakiba Nasiri.
The present study was designed to investigate the effect of early and late administration of phenylephrine during ischemia against regional ischemia-reperfusion injuries in an isolated rat heart model. All animals were randomly divided into experimental groups: (I) IR (Ischemic/ reperfusion): the hearts underwent 35 min of regional ischemia followed by 60 min of reperfusion; (II) 5HD-IR-0: the hearts were perfused for 5 min with 5HD (5-hydroxydecanoate, specific mKATP channel blocker, 100 µM) at the onset of regional ischemia; (III) 5HD-IR-20: the hearts were perfused for 5 min with 5HD 20 min after regional ischemia; (IV) PE-IR-10: the hearts were perfused for 5 min with phenylephrine 10 min after regional ischemia; (V) PE-IR-30: the hearts were perfused...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenylephrine; Ischemia; Reperfusion; Preconditioning; Cardioprotection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100561

Grandisin induces apoptosis in leukemic K562 cells
Provedor de dados: 60
Autores: Cortez,Alane Pereira; Menezes,Elizabeth Gomes Paulino; Benfica,Polyana Lopes; Santos,Alexandre Pereira dos; Cleres,Larissa Moreira; Ribeiro,Higor de Oliveira; Lima,Eliana Martins; Kato,Massuo Jorge; Valadares,Marize Campos.
Abstract In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 µM) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both methods, cellular viability was concentration-dependent and the IC50 values were lower than 0.85µM. Analysis of K562 cells after treatment with grandisin showed that the cell cycle was arrested in the G1 phase with a 12.31% increase, while both S and G2 phases decreased. Morphological studies conducted after the exposure of K562 to grandisin revealed changes consistent with the apoptosis process, which was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grandisin/antileukemic activities; Leukemic; K562; Apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100610

Carotene and provitamin A content of vegetables sold in Viçosa, MG, Brazil, during spring and winter
Provedor de dados: 60
Autores: Cardoso,Pollyanna Costa; Della Lucia,Ceres Mattos; Stringheta,Paulo César; Chaves,José Benício Paes; Pinheiro-Sant'Ana,Helena Maria.
This study investigated the α- and β-carotene content and provitamin A value of four leafy vegetables sold at local and street markets in Viçosa, MG, Brazil, in the spring and winter of 2002. Carotenoids were analyzed by high-performance liquid chromatography. α-Carotene was detected in all samples sold during spring, but was only present in a few samples of smooth and curly lettuce and kale in winter. β-Carotene was found in marked quantities in all leafy vegetables analyzed. Duncan's test (α = 5%) showed significantly higher α-carotene content in curly lettuce and vitamin A value in large-leaved watercress in the spring. Mean β-carotene content and vitamin A value were 7544, 8751, 2584, 2792, 8193, and 5338 μg/100 g and 666, 760, 227, 238, 698, and 460...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-Carotene; Β-Carotene; Leafy vegetables/analysis; Provitamin A.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300019

Thermal behavior and decomposition kinetics of rifampicin polymorphs under isothermal and non-isothermal conditions
Provedor de dados: 60
Autores: Alves,Ricardo; Reis,Thaís Vitória da Silva; Silva,Luis Carlos Cides da; Storpírtis,Silvia; Mercuri,Lucildes Pita; Matos,Jivaldo do Rosário.
The thermal behavior of two polymorphic forms of rifampicin was studied by DSC and TG/DTG. The thermoanalytical results clearly showed the differences between the two crystalline forms. Polymorph I was the most thermally stable form, the DSC curve showed no fusion for this species and the thermal decomposition process occurred around 245 ºC. The DSC curve of polymorph II showed two consecutive events, an endothermic event (Tpeak = 193.9 ºC) and one exothermic event (Tpeak = 209.4 ºC), due to a melting process followed by recrystallization, which was attributed to the conversion of form II to form I. Isothermal and non-isothermal thermogravimetric methods were used to determine the kinetic parameters of the thermal decomposition process. For non-isothermal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rifampicin; Tuberculosis; Thermal analysis; Thermal decomposition; Kinetic analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200022

Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes
Provedor de dados: 60
Autores: Pereira,Luciana Lopes Silva; Marcussi,Silvana; Sátiro,Lívia Cabral; Pereira,Chrystian Araujo; Andrade,Larissa Fonseca; Davide,Lisete Chamma; Santos,Custódio Donizete dos.
This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 ºC for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; White bean flour/genotoxic effects; Comet assay/toxicological analysis of food; Food/toxicological analysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100012

Physico-chemical and structural characterization of mucilage isolated from seeds of Diospyros melonoxylon Roxb.
Provedor de dados: 60
Autores: Singh,Sudarshan; Bothara,Sunil B.
Mucilage was isolated from the seeds of Diospyros melonoxylonRoxb., a plant growing naturally in the forests of India. Various physico-chemical methods like particle analysis, scanning electron microscopy, differential scanning calorimetry, differential thermal analysis, thermogravimetry analysis, molecular weight by gel permeation chromatography, rheometry, elemental analysis, x-ray diffraction spectrometry, zeta potential, fourier transform infrared spectroscopy, 1D(1H and 13C) (NMR) have been employed to characterize this gum in the present study. Particle analyses suggest that mucilage had particle size in nanometer. SEM analysis suggested that the mucilage had irregular particle size. The glass transition temperature of the gum observed was 78 °C and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diospyros melonoxylonRoxb./phytochemistry; Diospyros melonoxylonRoxb./physicochemical characterization; Zeta potential; Thermogravimetry analysis; 1D NMR spectroscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400713

Action of mouthwashes on Staphylococcus spp: isolated in the saliva of community and hospitalized individuals
Provedor de dados: 60
Autores: Andrade,Denise de; Souza,Paula Regina de; Beraldo,Carolina Contador; Watanabe,Evandro; Lima,Mery Elen; Haas,Vanderlei José.
The use of mouthwashes in critical patients has been a source of concern for health professionals due to the diverse range of products, causing uncertainty about which is the most indicated. This study aimed to assess the susceptibility of Staphylococcus spp. isolated in the saliva of individuals from the community and patients in Intensive Care Units (ICU) as to antiseptic mouthwashes. The following oral antiseptics were assessed: cetylpyridinium chloride solution, Listerine® and Neen®. Calcium alginate swab was used for saliva collection to isolate Staphylococcus spp. Microbiological processing involved growth, isolation, strain identification and determination of MID (maximum inhibitory dilution). MID was considered the greatest dilution that completely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral health; Staphylococcus spp; Mouthwashes; Oral hygiene.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300021

A suppository-base-matrix tablet for time-dependent colon-specific delivery system
Provedor de dados: 60
Autores: Zou,Meijuan; Wang,Caixia; Zhang,Xuezhu; Xu,Tao; Han,Jiarong; Zhang,Xiao; Cheng,Gang.
Our research has focused on the main design features and release performances of time-dependent colon-specific (TDCS) delivery tablets, which relies on the relative constancy that is observed in the small intestinal transit time of dosage forms. But inflammatory bowel disease（IBD&amp;#65289;can affect the transit time, and usually results in watery stool. Compared to the TDCS and wax-matrix TDCS tablet, a promising time-dependent colon-specific delivery system was investigated. In our study, a suppository-base-matrix coated tablet was evaluated. Water soluble suppository-base helps the expansion of tablet, facilitates uniform film dissolution and achives high osmotic pressure. Combining the expansion of carboxymethyl starch sodium (CMS-Na) and the moisture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammatory bowel disease; Lonicera japonica Thunb/extract; Suppository/colon-specific delivery system; Drugs/colon-specific delivery system; Tablets/colon-specific delivery system.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300535

Adherence and/or discontinuation of imatinib mesylate in patients with chronic myeloid leukemia
Provedor de dados: 60
Autores: Alves,Alexandra Rodrigues; Lima,William Gustavo; Nagai,Michelly Martins; Rodrigues,João Paulo Vilela; Ayres,Lorena Rocha.
ABSTRACT Adherence to imatinib mesylate improves clinical outcomes and promotes a reduction in health expenditure. However, treatment duration and lack of efficacy decrease adherence to pharmacotherapy, resulting in increased mortality associated with Chronic Myeloid Leukemia. This study aimed to evaluate and compare adherence and/or discontinuation of imatinib mesylate in different studies from the literature. An integrative review of original articles published between the years of 2004 and 2014 was performed using the databases PubMed/MEDLINE, Scopus and SciELO. The descriptor "imatinib" was used in two combinations employing the connector AND between terms: "medication adherence'' AND ''imatinib" AND "leukemia'' and ''patient compliance'' AND...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic Myeloid Leukemia/treatment; Imatinib Mesylate/treatment adherence; Medication adherence; Pharmacotheraphy review.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400581

Study of sensory-motor and somatic development of the offspring of rats (Wistar) treated with caffeine
Provedor de dados: 60
Autores: Silva,Elthon Gomes Fernandes da; Alexandre,Alessandro Aires; Nascimento,Gerlane Karla Bezerra Oliveira; Oliveira,Jaciel Benedito de; Neves,Elizabeth da Silveira; Costa Sobrinho,Austregézilo Vieira da; Silveira,Maria de Fátima Galdino da.
The influence of caffeine, administered to rats, on the somatic and sensory-motor development of the offspring was investigated. Female Wistar rats were divided into a control group and a treated group and received drinking water and a 0.1% solution of caffeine orally, respectively. The offspring, also divided into a control group and a treated group, received daily monitoring until the 20th day of life to verify alterations in somatic neural development. The offspring of the treated group had reduced weight on the day of birth and on the 1st, 5th, 15th and 20th days of life; shorter snout-anus length (evaluation done daily); shorter snout-tail length on the day of birth and on the 1st, 5th and 10th days of life, and signs of retardation of somatic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caffeine/effects; Postnatal development; Central nervous system/stimulants.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400019

Advances and challenges in therapeutic monoclonal antibodies drug development
Provedor de dados: 60
Autores: Santos,Mariana Lopes dos; Quintilio,Wagner; Manieri,Tania Maria; Tsuruta,Lilian Rumi; Moro,Ana Maria.
The use of serum containing polyclonal antibodies from animals immunized with toxins marked the beginning of the application of antibody-based therapy in late nineteenth century. Advances in basic research led to the development of the hybridoma technology in 1975. Eleven years later, the first therapeutic monoclonal antibody (mAb) was approved, and since then, driven by technological advances, the development of mAbs has played a prominent role in the pharmaceutical industry. In this review, we present the developments to circumvent problems of safety and efficacy arising from the murine origin of the first mAbs and generate structures more similar to human antibodies. As of October 2017, there are 61 mAbs and 11 Fc-fusion proteins in clinical use. An...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunotherapy; CDR grafting; Phage display; Transgenic mice; Single B cell sorting.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700406

Lupeol and its esters: NMR, powder XRD data and in vitro evaluation of cancer cell growth
Provedor de dados: 60
Autores: Silva,Aline Teixeira Maciel e; Magalhães,Cássia Gonçalves; Duarte,Lucienir Pains; Mussel,Wagner da Nova; Ruiz,Ana Lucia Tasca Gois; Shiozawa,Larissa; Carvalho,João Ernesto de; Trindade,Izabel Cristina; Vieira Filho,Sidney Augusto.
ABSTRACT The triterpene lupeol (1) and some of its esters are secondary metabolites produced by species of Celastraceae family, which have being associated with cytotoxic activity. We report herein the isolation of 1, the semi-synthesis of eight lupeol esters and the evaluation of their in vitro activity against nine strains of cancer cells. The reaction of carboxylic acids with 1 and DIC/DMAP was used to obtain lupeol stearate (2), lupeol palmitate (3) lupeol miristate (4), and the new esters lupeol laurate (5), lupeol caprate (6), lupeol caprilate (7), lupeol caproate (8) and lupeol 3’,4’-dimethoxybenzoate (9), with high yields. Compounds 1-9 were identified using FT-IR, 1H, 13C-NMR, CHN analysis and XRD data and were tested in vitro for proliferation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lupeol/in vitro evaluation; Lupeol ester; K-562 cells; XRD method; Antiproliferative effect.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300621

Pancreatic beta cell protection/regeneration with phytotherapy
Provedor de dados: 60
Autores: Hosseini,Azar; Shafiee-Nick,Reza; Ghorbani,Ahmad.
Although currently available drugs are useful in controlling early onset complications of diabetes, serious late onset complications appear in a large number of patients. Considering the physiopathology of diabetes, preventing beta cell degeneration and stimulating the endogenous regeneration of islets will be essential approaches for the treatment of insulin-dependent diabetes mellitus. The current review focused on phytochemicals, the antidiabetic effect of which has been proved by pancreatic beta cell protection/regeneration. Among the hundreds of plants that have been investigated for diabetes, a small fraction has shown the regenerative property and was described in this paper. Processes of pancreatic beta cell degeneration and regeneration were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus/treatment; Diabetes mellitus/phytotherapy; Phytotherapy/Diabetes mellitus; Islets/endogenous regeneration; Beta cells/protection; Beta cells/regeneration; Pancreas; Medicinal plants/regenerative properties.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100001

Formulation and evaluation of CFC free inhalers for beclomethasone dipropionate
Provedor de dados: 60
Autores: Talasila,Gopala Krishna Murthy; Mukkala,Bala Vishnu Priya; Vattikuri,Satyanarayana.
Beclomethasone dipropionate CFC free inhalation formulations were developed with a view to treat asthma prophylactically. Dry powder inhalers (DPI) for beclomethasone dipropionate were prepared with different grades of lactose monohydrate. The influence of carrier and overages on performance of DPI was studied. Metered dose inhalers (MDI) with HFA based propellants were formulated with various doses, overages and different concentrations of alcohol. Formulated DPI and MDI were evaluated for various official and unofficial quality control tests. The influence of over doses on valve delivery, effect of overages on emitted dose and influence of alcohol on spray pattern from MDI were studied. The better fine particle fraction and emitted dose were obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beclomethasone dipropionate Dry powder inhalers Metered dose inhalers; ; Emmited dose Fine particle fraction; Overages.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200004

Salacia campestris root bark extract: peroxidase inhibition, antioxidant and antiradical profile
Provedor de dados: 60
Autores: Vellosa,José Carlos Rebuglio; Khalil,Najeh Maissar; Gutierres,Vânia Ortega; Santos,Vânia Aparecida de Freitas Formenton Macedo dos; Furlan,Maysa; Brunetti,Iguatemy Lourenço; Oliveira,Olga Maria Mascarenhas de Faria.
Reactive oxygen species (ROS) and free radical species have been implicated in initiating or accompanying many diseases in living organisms; there is thus, a continual need for antioxidants molecules to inactivate ROS/free radicals. Many studies of plants crude extracts have demonstrated free-radical scavenging and antioxidant action. Salacia species have long been used, in several countries, as traditional medicines against certain diseases and for their anti-inflammatory properties. In this study, Salacia campestris Walp (Hippocrateaceae) root bark ethanol extract (ScEtOH) was assessed for its ability to scavenge free radicals and reactive oxygen species; the results were expressed as percentage inhibition of the active species. ScEtOH was efficient...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salacia campestris Walp/antioxidant properties; Hippocrateaceae; Myeloperoxidase; Reactive oxygen species; Antioxidants; Free radicals.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100012

Synthesis and DPPH scavenging assay of reserpine analogues, computational studies and in silico docking studies in AChE and BChE responsible for Alzheimer's disease
Provedor de dados: 60
Autores: Yar,Muhammad; Arshad,Muhammad; Farooq,Ariba; Gilani,Mazhar Amjad; Ayub,Khurshid; Ejaz,Asma; Kumar,Anupriya; Ninomiya,Ichiya.
Alzheimer's disease (AD) is a fast growing neurodegenerative disorder of the central nervous system and anti-oxidants can be used to help suppress the oxidative stress caused by the free radicals that are responsible for AD. A series of selected synthetic indole derivatives were biologically evaluated to identify potent new antioxidants. Most of the evaluated compounds showed significant to modest antioxidant properties (IC50 value 399.07 140.0±50 µM). Density Functional Theory (DFT) studies were carried out on the compounds and their corresponding free radicals. Differences in the energy of the parent compounds and their corresponding free radicals provided a good justification for the trend found in their IC50 values. In silico, docking of compounds into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alzheimer's disease; Antioxidant compounds; Chronic diseases; Density Functional Theory; Molecular docking; Computational studies; DPPH assay; Indole derivatives; Acetylcholinesterase (AChE); Butyrylcholinesterase (BChE).
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100053

Chemical composition and bioactivity of methanolic extract obtained from Lepista sordida
Provedor de dados: 60
Autores: Acharya,Krishnendu; Ghosh,Sandipta; Biswas,Rhituparna.
The present study aimed at the evaluation of chemical composition and bioactive potential of methanolic extract obtained from Lepista sordida in terms of antioxidative and antimicrobial efficacy. The macrofungus is recognized for its high nutritional value and medicinal properties. However, to the best of our knowledge bioactivity of its methanolic extract is yet to be explored. In this investigation, quantitative analysis of mycochemicals revealed the extract contained significant amount of phenolic compounds such as phenols and flavonoids. Ascorbic acid was found in higher amount than β carotene and lycopene which were present in vestigial amounts. A phenolic profile was also determined using high performance liquid chromatography that further confirmed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial; Free radical scavenger; High performance liquid chromatography; Mycotaxon; Mycochemicals.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100600

Cytotoxic and apoptotic potential of Myristica fragrans Houtt. (mace) extract on human oral epidermal carcinoma KB cell lines
Provedor de dados: 60
Autores: Rengasamy,Gayathri; Venkataraman,Anuradha; Veeraraghavan,Vishnu Priya; Jainu,Mallika.
Several studies have revealed that certain naturally occurring medicinal plants inhibit the growth of various cancers. The present study was conducted to evaluate cytotoxicity and apoptotic induction potential of Myristica fragrans Houtt mace extract. The cytotoxic activity of the Myristica fragrans Houtt mace acetone extract was assayed by MTT assay on human oral epidermal carcinoma KB cell lines. KB cells were incubated with different concentration of mace extract ranging from 25 to 125 μg/mL for 24hrs. The apoptotic induction potential was also studied by the analysis of Bcl-2 protein and gene expression in mace extract incubated KB cell lines using western blotting technique and real-time polymerase chain reaction. The mace extract exhibited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myristica fragrans Houtt; (mace)/extract; Apoptosis; Cytotoxicity; KB cell lines; MTT assay; Bcl-2 gene.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300629

Implementation of vancomycin dosing nomogram in an electronic prescribing system: an innovative tool in antibiotic stewardship
Provedor de dados: 60
Autores: Lima,Tácio de Mendonça; Elias,Sabrina Calil; Estrela,Rita de Cássia Elias; Cardoso,Fernando Luiz Lopes.
Vancomycin (VAN) is the gold standard therapy for Methicillin-resistant Staphylococcus aureus(MRSA) infections such as bacteremia and endocarditis. However, VAN suboptimal dosing for serious infections caused by S. aureus isolates that have elevated minimum inhibitory concentration (MIC), could be associated with poor outcome. Better understanding of VAN pharmacokinetics and pharmacodynamics (PK/PD) has led to the creation of new recommendations with optimized dosing regimens for the treatment of MRSA infections. For severe infectious, such as pneumonia and endocarditis, a VAN serum trough concentration of 15-20 mg/L at the steady state should be targeted. The aim of this study was to show how a nomogram with updated VAN dosing was devised and how it was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vancomycin/prescribing; Methicillin/use; Staphylococcus aureus/methicillin-resistant; Electronic prescribing system/application in hospital; Antibiotics/rational use; Antimicrobials/rational use.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300567

Metabolic analysis of the effect of rheum on a taurocholate-induced acute pancreatitis rat model
Provedor de dados: 60
Autores: Chen,Zhonghua; Shen,Li; Yang,Changyong; Jiang,Jinghui; Liu,Xing.
ABSTRACT The effects of rheum on serum parameters in a taurocholate-induced acute pancreatitis (AP) rat model were investigated using pathological and biochemical tests, and a proton nuclear magnetic resonance (1H NMR)-based metabonomic strategy. Healthy rats and rats with AP were either treated with rheum (7.5% at a dose of 1.5 g/kg) or left untreated. Serum samples were collected from the AP and rheum-treated groups at 6, 12, and 24 h after treatment. The effect of rheum on pathological changes in the pancreatic was investigated to validate the AP model. We obtained 1H NMR spectra and analyzed the results using the partial least squares discriminant method. The results of the pathological and metabolic analyses revealed an amelioration of multiple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheum/effects; Acute pancreatitis/analysis; Metabolomics; 1H NMR; Pathological changes; Rats.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200633

Antitumor efficacy of EDTA co-treatment with cisplatin in tumor-bearing mice
Provedor de dados: 60
Autores: El-Naggar,Sabry Ali; El-Said,Karim Samy.
Ethylenediamine tetraacetic acid (EDTA) is used in various medical applications. The aim of this study is to investigate the antitumor efficacy of EDTA alone or with cisplatin (Cis). Fifty male albino mice were used to assess the median lethal dose (LD50) of EDTA via intraperitoneal (i.p) injection. To determine the antitumor activity, fifty female albino mice were divided into five groups as the following; Group 1 (Gp1) was negative control; (Gp2-5) inoculated i.p with 2×106 Ehrlich Ascites Carcinoma (EAC) cells/mouse. After one day, Gp3, Gp4 and Gp5 injected with Cis (2 mg/kg), EDTA (25 mg/kg) and Cis (2 mg/kg)/EDTA (25 mg/kg) for six days, respectively. At day 14, all groups were sacrificed to assess the tumor profile, liver enzymes (alanine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Ethylenediamine tetraacetic acid (EDTA)/Antitumor efficacy; Ehrlich Ascites Carcinoma.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100571

Development of mouthwash with Rosmarinus officinalis extract
Provedor de dados: 60
Autores: Paula,Isabela Moreira Baumgratz de; Moraes,Flávia Costa; Souza,Orlando Vieira de; Yamamoto,Célia Hitomi.
Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quality of the drugs were carried out, quantifying phenols and flavonoids as chemical markers. Mouthwash solutions were formulated containing 0.1, 5 and 10% ethanol extract of R. officinalis; and 0.05, 5 and 10% of the hexane fraction of R. officinalis. In order to evaluate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosmarinus officinalis/pharmacognosy; Rosmarinus officinalis/Anti-microbial activity; Mouthwash; Periodontics/use of mouthwash; Medicinal plants.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400851

Changes in hepatic metabolic enzyme activities and biliary excretion of 4-nitrophenol in streptozotocin induced diabetic rats
Provedor de dados: 60
Autores: Almási,Attila; Pinto,Évora da Ibéria Leite Nogueira; Kovács,Noémi-Piroska; Fischer,Tamás; Markovics,Zoltán; Fischer,Emil; Perjési,Pál.
Abstract Activity of hepatic metabolic enzymes of glucuronidation and sulfation of 4-nitrophenol (PNP) and biliary excretion of its glucuronide (PNP-G) and sulfate (PNP-S) conjugates have been investigated in control and streptozotocin (STZ)-induced diabetic rats. 500 μM PNP solution was luminally perfused in a cannulated jejunal loop for 90 minutes. It was found that biliary excretion of PNP-G was significantly decreased in the diabetic rats. This effect of STZ could be completely reversed by administration of rapid-acting insulin. Activity of hepatic UDP-glucuronyltransferase and β-glucuronidase was also depressed by the STZ pretreatment. Administration of insulin antagonized the inhibitory action of STZ on UDP-glucuronyltransferase, but the reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-Nitrophenol; Hepatic excretion; Glucuronide conjugate; Sulfate conjugate; Experimental diabetes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100615

Pharmaceutical clinical services in basic care in a region of the municipality of São Paulo
Provedor de dados: 60
Autores: Santos,Felipe Tadeu Carvalho; Silva,Dayde Lane Mendonça da; Tavares,Noemia Urruth Leão.
Pharmaceutical care has undergone several transformations in the health context over the years. Thus, the pharmacist has suffered a reconfiguration of his performance, mainly with the incorporation of clinical services and patient approach. The study analyzed the results of the implementation of pharmaceutical clinical services in Primary Health Care, through the use of indicators of supply, demand and productivity, clinical and process quality related to pharmaceutical care. We included all the clinical visits (n=1,833) performed to 1,080 users in 12 Basic Health Unit facilities from May to November 2016, of which 40.8% (n=748) were consultations in the establishments and 50.2% (n=1,085) home visits. Most patients (73.5%) were referred by team and 17.5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care/primary care; Chronic health/conditions; Pharmacists/professional practice/Brazil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300604

Effects of maternal enflurane exposure on NR2B expression in the hippocampus of their offspring
Provedor de dados: 60
Autores: Luo,Fo-Quan; Liu,Jun-Wu; Tang,Shu-Xin; Zhao,Wei-Lu; Hu,Yan; Xu,Lin; Li,Meng-Yuan.
This work aims to study the pathogenesis of learning and memory impairment in offspring rats resulting from maternal enflurane anesthesia by focusing on the expression of the N-methyl-d-aspartic acid receptor subunit 2B (NR2B) in the hippocampus of the offspring. Thirty female Sprague-Dawley rats were randomly divided into three groups: control (C group), 4 h enflurane exposure (E1 group), and 8 h enflurane exposure (E2 group) groups. Eight to ten days after the initiation of pregnancy, rats from the E1 and E2 groups were allowed to inhale 1.7% enflurane in 2 L/min oxygen for 4 h and 8 h, respectively. Rats from the C group were allowed to inhale 2 L/min of oxygen only. The Morris water maze was used to assay the learning and memory function of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enflurane/use/maternal anesthesia; Enflurane/adverse effects/experimental study; Enflurane/effects/pregnancy; N-methyl-D-aspartic acid/receptor/gene expression.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300673

Cardiovascular risk rate in hypertensive patients attended in primary health care units: the influence of pharmaceutical care
Provedor de dados: 60
Autores: Firmino,Paulo Yuri Milen; Vasconcelos,Tainá Osterno; Ferreira,Caroline Carneiro; Moreira,Larissa Mendonça; Romero,Nirla Rodrigues; Dias,Lilian Araújo; Queiroz,Maria Goretti Rodrigues de; Lopes,Marcos Venícios de Oliveira; Fonteles,Marta Maria de França.
Cardiovascular complications are relevant due to their frequency and severity on the hypertension scenario. Studies refer Pharmaceutical Care (PC) as capable of decreasing cardiovascular risk rate (%CVR) on hypertensive patients. This study aimed to investigate, through a randomized clinical assay, the influence of PC service on the %CVR of hypertensive patients assisted in a health primary care unit from Fortaleza-Ceará. Two study groups were formed: i. Intervention Group (IG), which received orientation about taking medicines, actions aiming to prevent/solve medicine interactions and adverse effects and non-pharmacological interventions for 9 months and, ii. Control Group (CG), which received traditional assistance of the unit and was monitored during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care; Cardiovascular risk; Hypertension.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300617

Memory retrieval improvement by Heteropterys aphrodisiaca in aging rats
Provedor de dados: 60
Autores: Galvão,Suzana Maria Pereira; Mendes,Fúlvio Rieli; Oliveira,Maria Gabriela Menezes de; Mattei,Rita; Mello,João Carlos Palazzo de; Roman Júnior,Walter Antonio; Carlini,Elisaldo de Araújo.
Few data exists about the pharmacological properties of Heteropterys aphrodisiaca O. Mach. (Malpighiaceae), which is native to the scrubland regions of Brazil. The present study investigated the effects of oral treatment with H. aphrodisiaca extract (BST0298) on the learning and memory of young (3-6 months) and aged (21-23 months) rats, and compared the in vitro antioxidant activity of three lots collected in different years. An improvement in the number of sessions to learn the task was observed in the left/right discrimination test in aged rats treated for 45 days with 25 mg/kg (7.0 ± 0.5; p=0.005) or 50 mg/kg (7.6 ± 0.6; p=0.012) compared with control old rats (11.0 ± 1.6). On the other hand, pre-treatment did not improve the performance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heteropterys aphrodisiaca O. Mach/pharmacognosy; Heteropterys aphrodisiaca O. Mach/antioxidant activity; Malpighiaceae/pharmacognosy; Antioxidants; Flavonoids; Plant extract.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400020

Evaluation of in vitro and in vivo therapeutic efficacy of Ribes alpestre Decne in Rheumatoid arthritis
Provedor de dados: 60
Autores: Hasan,Alamgeer Umme Habiba.
Rheumatoid arthritis is an autoimmune inflammatory disorder, despite the discovery of numerous drugs there is still need to introduce newer, safer and more effective sources of drugs such as medicinal herbs. Present research work was an attempt to appraise the antiarthritic potential of Ribes alpestre Decne in rheumatoid arthritis. In vitro inhibition of protein (bovine serum albumin and egg albumin) denaturation, Human red blood cell membrane stabilization assays along with formaldehyde induced arthritis in rats were commenced in this study. Findings of present investigation demonstrated significant and dose dependent antiarthritic effect of Ribes alpestre. Aqueous ethanolic extract, butanol and aqueous fraction illustrated 95%, 69.233% and 92.840%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthritis; Ribes alpestre; Protein denaturation; Membrane stabilization; Formaldehyde.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100590

Sleep quality and OPRM1 polymorphisms: a cross-sectional study among opioid-naive individuals
Provedor de dados: 60
Autores: Zahari,Zalina; Ibrahim,Muslih Abdulkarim; Musa,Nurfadhlina; Tan,Soo Choon; Mohamad,Nasir; Ismail,Rusli.
ABSTRACT Opioidergic system involves in regulation of sleep and wakefulness. It is possible, therefore, that genetic polymorphisms in OPRM1 influence sleep quality. This study investigated the association of OPRM1 polymorphisms with subjective sleep quality among opioid-naive individuals. This cross-sectional observational study involved 161 opioid-naive males (mean age = 27.74 years; range: 18−63 years). Subjective sleep quality was assessed with the translated and validated Malay version of the Pittsburgh Sleep Quality Index (PSQI). DNA was extracted from whole blood and subjected to polymerase chain reaction (PCR)-genotyping for two OPRM1 polymorphisms (118A&gt;G and IVS2+691G&gt;C). Subjects with combined 118A and IVS2+691G alleles (AC haplotype) had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep-Wake Transition Disorders; Sleep quality; Sleep/genetic; Polymorphism; Pittsburgh Sleep Quality Index; OPRM1; Opioid receptors /m analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100610

Development and validation of a fluorimetric method to determine curcumin in lipid and polymeric nanocapsule suspensions
Provedor de dados: 60
Autores: Mazzarino,Letícia; Bellettini,Ismael Casagrande; Minatti,Edson; Lemos-Senna,Elenara.
A simple, rapid, and sensitive fluorimetric method was developed and validated to quantify curcumin in lipid and polymeric nanocapsule suspensions, using acetonitrile as a solvent. The excitation and emission wavelengths were set at 397 nm and 508 nm, respectively. The calibration graph was linear from 0.1 to 0.6 µg/mL with a correlation coefficient of 0.9982. The detection and quantitation limits were 0.03 and 0.10 µg/mL, respectively. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of curcumin content in lipid and polymeric nanocapsule suspensions during the early stage of formulation development.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin/determination; Fluorimetric method/quantitative analysis; Polymeric nanocapsules/evaluation; Lipid nanocapsules/evaluation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200008

Application of multivariate techniques in the evaluation of melt granulation products
Provedor de dados: 60
Autores: Ochoa Andrade,Ana Teresa.
In this work, principal component analysis and cluster analysis were applied as helping tools to extract useful information in the development of formulations and manufacturing processes of melt granulates. Two melt granulation processes that differ in the shear stress applied to the solid bed during melting step were designed. These processes employ equipment frequently used in the local pharmaceutical industry. The selected binders include both hydrophilic and hydrophobic excipients, which were used alone or in binary mixtures. Granulates were characterized regarding their physicomechanical properties, including their compaction behavior. The resulting tablets were also evaluated. The selected multivariate statistical methods proved to be useful in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Granulates/evaluation; Tablets/evaluation; Granulates/manufacturing processes; Melt granulates/manufacturing processes; Tablets/production processes; Principal component analysis/application; Cluster analysis/application; Pharmaceutical formulations/development; Melt granulation/application.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400010

Powder properties of binary mixtures of chloroquine phosphate with lactose and dicalcium phosphate
Provedor de dados: 60
Autores: Odeniyi,Michael Ayodele; Onyenaka,Collins Chidi; Itiola,Oludele Adelanwa.
A study was conducted on the packing and cohesive properties of chloroquine phosphate in binary mixtures with lactose and dicalcium phosphate powders. The maximum volume reduction due to packing as expressed by the Kawakita constant, a, and the angle of internal flow, θ, were the assessment parameters. The individual powders were characterized for their particle size and shape using an optical microscope. Binary mixtures of various proportions of chloroquine phosphate with lactose and dicalcium phosphate powders were prepared. The bulk and tapped densities, angles of repose and internal flow, as well as compressibility index of the materials were determined using appropriate parameters. The calculated and determined values of maximum volume reduction for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chloroquine phosphate; Lactose; Dicalcium phosphate; Drugs/delivery systems; Drugs/production; Drugs/packing; Drugs/characterization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300017

Antimicrobial susceptibility profile and research of mec A and erm genes in coagulase-negative staphylococci isolated from platelet concentrates bags
Provedor de dados: 60
Autores: Martini,Rosiéli; Hörner,Rosmari; Graichen,Daniel Ângelo Sganzerla.
Abstract In recent years, several studies have described the clinical impact of bacterial infection associated with transfusion of platelet concentrates (PCs). Among the blood components, PCs are responsible for the highest rates of bacterial contamination as well as septic transfusion reactions. We assessed antimicrobial susceptibility profile, resistance to methicillin (MRCoNS), and resistance to macrolides, lincosamides and streptogramins of group B (MLSB) of 16 coagulase-negative staphylococci (CoNS) isolates from an investigation in 691 PCs bags. We then compared conventional and automated phenotypic methods, disc diffusion test (DD) and VITEK(r) 2, respectively as well as phenotypic and genotypic methods (Polymerase Chain Reaction - PCR). All CoNS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial resistance; Staphylococcus sp; Platelet concentrates; MecA; Erm.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100609

Observation of preparation and administration of drugs by nursing assistants in patients with enteral feeding tube
Provedor de dados: 60
Autores: Heydrich,Joana; Heineck,Isabela; Bueno,Denise.
This study aimed to know the standard of preparation and administration of medicines to patients using probes of enteral nutrition (SNE) hospitalized in a university hospital in Porto Alegre. Were accompanied sixty nursing auxiliaries, in two units of hospital, in their routine work and observed their techniques. The practice was compared with procedures that are part of the rules for administration of medicines to patients on enteral therapy. The auxiliary held a grinding in 90% of cases of solid pharmaceutical preparation. For this procedure they used preferably the mortar and pistil (63.3%). The dilution of the drugs was made mainly with tap water (78.3%). Most professionals (91.1%) prepare and administer together all medications that were prescribed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteral nutrition; Drugs/administration; Nursing/practice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100014

Titrimetric and spectrophotometric assay of diethylcarbamazine citrate in formulations using iodate and iodide mixture as reagents
Provedor de dados: 60
Autores: Swamy,Nagaraju; Prashanth,Kudige Nagaraj; Basavaiah,Kanakapura.
One titrimetric and two spectrophotometric methods are proposed for the determination of diethylcarbamazine citrate (DEC) in bulk drug and in formulations using potassium iodate and potassium iodide as reagent. The methods employ the well-known analytical reaction between iodate and iodide in the presence of acid. In titrimetry (method A), the drug was treated with a measured excess of thiosulfate in the presence of unmeasured excess of iodate-iodide mixture and after a standing time of 10 min, the surplus thiosulfate was determined by back titration with iodine towards starch end point. Titrimetric assay is based on a 1:3 reaction stoichiometry between DEC and iodine and the method is applicable over 2.0-10.0 mg range. The liberated iodine is measured...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diethylcarbamazine citrate/determination; Titrimetry/quantitative analysis; Spectrophotometry/quantitative analysis; Pharmaceutical formulations/analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100043

Effects of depomedroxyprogesterone acetate on the development and maintenance of Candida albicans in the vagina of oophorectomized Wistar rats (Rattus norvegicus)
Provedor de dados: 60
Autores: Storti-Filho,Agenor; Damke,Edilson; Carrara,Márcia Aparecida; Batista,Marcia Regina; Donatti,Lucélia; Boer,Cinthia Gandolfi; Svizdinski,Terezinha Inez Estivalet; Consolaro,Márcia Edilaine Lopes.
The objective of this study was to determine the effect of medroxyprogesterone acetate (DMPA) on the development and maintenance of Candida albicans in the vagina of oophorectomized Wistar rats. The animals were divided into negative control groups (NCG), which received injections of sterile saline; positive control groups (PCG), which were given injections of estradiol valerate; and progesterone groups (PG), which were given injections of Depo-Provera®. After one week of hormonal induction, vaginal infection by C. albicans was induced in all the groups and detected by vaginal yeast culture and Papanicolaou smear. In addition, scanning and transmission electron microscopy images were obtained to confirm the vaginal infection by yeast in PG. A difference in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depomedroxyprogesterone acetate/effects; Depomedroxyprogesterone acetate/experimental study; Vulvovaginal candidiasis; Candida albicans.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100021

Knowledge and conduct of pharmacists for dispensing of drugs in community pharmacies: a cross-sectional study
Provedor de dados: 60
Autores: Reis,Tiago Marques dos; Guidoni,Camilo Molino; Girotto,Edmarlon; Rascado,Ricardo Radigheri; Mastroianni,Patrícia de Carvalho; Cruciol,Joice Mara; Pereira,Leonardo Régis Leira.
The objective of the study was to evaluate the knowledge of pharmacists and check their conduct in relation to dispensing of drugs. This is a cross-sectional study performed in four municipalities from South and Southeast of Brazil, which are reference in health national scenario and concentrate 73.6% of national economic activity. Pharmacists who works in community pharmacies were invited to answer a questionnaire prepared by the authors of this study and validated. The main outcome measured was the pharmaceutical knowledge, rated according to the number of correct answers on that questionnaire prepared by the researchers, and professional conduct in relation to dispensing of drugs. The data collection occurred from September to December 2012 and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacists/professional conduct; Medicines/dispensing Medicines/rational use; Community pharmacy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300733

The curative effects of methylsulfonylmethane against glycerol-induced acute renal failure in rats
Provedor de dados: 60
Autores: laham,Shaza Anwar Al.
Abstract The present study was performed to explore the curative effect of Methylsulfonylmethane (MSM) in an experimental model of myoglobinuric acute renal failure (ARF). In this experimental model, Rats were injected with 50% glycerol (10 mL/kg, im) followed by an hour later and daily in the next six days by MSM (400 mg/kg) or saline. Kidney’s function (urea and creatinine), and reduced glutathione were analyzed. A renal failure produced by glycerol injection, with a significant increase of blood urea and serum creatinine was observed. Rats that received MSM in addition to glycerol had significantly lower blood urea and serum creatinine levels compared to those receiving glycerol alone. However, glutathione has markedly increased after MSM treatment. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylsulfonylmethane (MSM)/curative effects; Acute renal failure; Myoglobinuria; Reactive oxygen species.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100617

Ameliorating the antitumor activity of lenalidomide using PLGA nanoparticles for the treatment of multiple myeloma
Provedor de dados: 60
Autores: Karri,Veera Venkata Satyanarayana Reddy; Dhandapani,Nagasamy Venkatesh; Mannemala,Sai Sandeep; Radhakrishna,Kollipara; Mulukutla,Shashank; Sudunagunta,Dedeepya.
Abstract Lenalidomide (LND) is an anti-cancer drug and an effective derivative of thalidomide used for multiple myeloma therapy. Because of its poor solubility in water, LND is known to cause low oral bioavailability (below 33%), and as a direct consequence of this, the dosing frequency is extended thus increasing risk of toxicity. To improve its bioavailability and sustained release, the present study aims to formulate polymeric nanoparticles (NPs) for LND using [Poly (lactic-co-glycolic acid)] (PLGA) as a polymer. The polymeric NPs were evaluated for particle size, SEM, XRD, drug content, entrapment efficiency (EE), in vitro release studies and in vivo bioavailability studies in rats. The formulated NPs possessed a size of 179±0.9 nm and a zeta potential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiple myeloma/treatment; Lenalidomide/uses; PLGA/sustained release; Polymeric nanoparticles/development; Solubility.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200602

Anti-diabetic effect of betulinic acid on streptozotocin-nicotinamide induced diabetic male mouse model
Provedor de dados: 60
Autores: Birgani,Golshan Arzani; Ahangarpour,Akram; Khorsandi,Layasadat; Moghaddam,Hadi Fathi.
ABSTRACT Diabetes is a metabolic disease caused by abnormal insulin secretion or action. In the present study, the effects of betulinic acid (BA, a triterpene) are evaluated on glucose, α-amylase and plasma insulin levels, insulin resistance and the histopathology of pancreatic islets in streptozotocin-nicotinamide (STZ-NA) diabetic mice. Seventy adult male NMRI mice were randomly divided into seven groups: control, sham, diabetic, diabetic treated with BA (10, 20 and 40 mg/kg) and diabetic treated with metformin (200 mg/kg). Diabetes was induced in mice by intraperitoneal injection of streptozotocin 50 mg/kg after a dose of nicotinamide 120 mg/kg. Two weeks after treatment with BA, blood samples were collected for measuring glucose, α-amylase and insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Betulinic acid; Diabetes; Mouse; Streptozotocin-nicotinamide.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200621

Effect of ethanol Adiantum capillus-veneris extract in experimental models of anxiety and depression
Provedor de dados: 60
Autores: Rabiei,Zahra; Setorki,Mahbubeh.
Depression is one of the most common psychiatric disorders with a prevalence of 15%-25%. Monoamine aminotransferases, in particular, norepinephrine, serotonin, and dopamine, change in the brain of depressed people. Adiantum capillus-veneris is one of the species of the maidenhair fern genus that have traditionally been used to treat cough, cold symptoms, and local hair loss.In this experimental study, white male rats weighing 250-300 g were assigned to 5 groups of 10 each; group 1: Receiving normal saline; groups 2-4: Receiving A.capillus-veneris extract at 50, 100, and 200 mg/kg, respectively; and group 5: Receiving fluoxetine at 10 mg/kg. Chronic unpredictable stress (CUS) was induced by 3-week exposure to chronic stress. The forced swim test and plus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adiantum capillus-veneris /extract/effects; Forced swim test.Anti-anxiety.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100554

Pharmacokinetic study of isoquercitrin in rat plasma after intravenous administration at three different doses
Provedor de dados: 60
Autores: Xue,Hefei; Li,Yuzhong; Zhang,Wenjie; Lu,Dongrui; Chen,Yinghui; Yin,Jingjing; Meng,Yihan; Ying,Xixiang; Kang,Tingguo.
The aim of this study is to develop a simple and specific HPLC method using vitexin as the internal standard to investigate the pharmacokinetics of isoquercitrin (ISOQ) after three different doses administrated intravenously to rats. The pharmacokinetic parameters were calculated by both compartmental and non-compartmental approaches. The results showed that ISOQ fitted a three-compartment open model. The values of AUC increased proportionally within the range of 5-10 mg·kg-1. Moreover, a half-life, b half-life, ªCL, MRT0-t and MRT0→&amp;#8734; of ISOQ in rats showed significant differences between 20 mg·kg-1 and other doses, indicating that ISOQ presented dose-dependent pharmacokinetics in the range of 5-10 mg·kg-1 and non-linear pharmacokinetics at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoquercitrin/pharmacokinetics; Isoquercitrin/experimental study; High Performance Liquid Chromatography; Crataegus pinnatifida Bge; Var major/leaves; Var major/phytochemistry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300005

Preparation and characterization of naftifine-loaded poly(vinyl alcohol)/sodium alginate electrospun nanofibers
Provedor de dados: 60
Autores: Esentürk,İmren; Balkan,Timuçin; Güngör,Sevgi; Saraç,Sezai; Erdal,Meryem Sedef.
In this study, naftifine (a topical antifungal drug) loaded poly(vinyl) alcohol (PVA)/sodium alginate (SA) nanofibrous mats were prepared using the single-needle electrospinning technique. The produced nanofibers were crosslinked with glutaraldehyde (GTA) vapor. The morphology and diameter of the electrospun nanofibers were studied by scanning electron microscopy (SEM). SEM images showed the smoothness of the nanofibers and indicated that the fiber diameter increased with crosslinking and drug loading. Atomic force microscopy (AFM) images confirmed the uniform production of the scaffolds, and elemental mapping via energy dispersive X-ray spectroscopy (EDS) showed the uniform distribution of the drug within the nanofibers. An attenuated total reflectance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naftifine; Nanofibers; Electrospinning; Topical drug delivery.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100606

Synthesis and evaluation of platinum complexes with potential antitumor activity
Provedor de dados: 60
Autores: Freire,Rachel Lima Marcelino; Marques,Maria Betânia de Freitas; Souza-Fagundes,Elaine Maria de; Oliveira,Renata Barbosa de; Alves,Ricardo José.
Abstract A novel series of platinum (II) complexes was synthesized and the complexes were evaluated for their in vitro cytotoxicity against four human cancer cells lines. Five platinum complexes showed activity against at least one tumor cell line. Complexes 3 and 6 were promising, being active, at micromolar concentrations, against all the assayed tumor cell lines. Compound 3 was selected for further studies in mice with Ehrlich solid tumors and it was able to reduce the rate of tumor growth significantly during the first seven days. However, at the end of the experiments, there was no significant difference between the group of animals treated with 3 and the control group. The low solubility of the compound in the assay conditions can explain, at least...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: /Cytotoxic activity; Platinum complexes/ Ehrlich solid tumor activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100612

Lack of effect of cell-wall targeted antibacterials on biofilm formation and antifungal susceptibility of Candidaspecies
Provedor de dados: 60
Autores: Leite,Gisela Myrian de Lima; Baeza,Lílian Cristiane; Ramos,Rosana Teixeira; Yamada,Sérgio Seiji; Magon,Thiago Ferreira dos Santos; Kimura,Elza; Svidzinski,Terezinha Inez Estivalet.
The use of central venous catheters (CVC) and broad-spectrum antibacterials are among the main risk factors for the development of candidemia in patients admitted to intensive care units (ICU). It is known that some antibacterials increase the resistance of these yeasts to azole antifungals. Thus, the aim of this research was to determine whether yeast present in CVC colonizations previously exposed to cell-wall targeted antibacterials benefit from a reduction in susceptibility to fluconazole and voriconazole, facilitating their ability to form biofilms. Candida albicans, C. tropicalis, C. glabrata, C. parapsilosis and C. guilhermondii were seeded into antibacterial (cefepime, meropenem, vancomycin, and piperacillin-tazobactam) gradient plates produced in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central venous cateter/use; Candidemia/control; Biofilm/formation; Antibacterials; Antifungals; Candida/species/control.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300467

In silico pharmacodynamics, toxicity profile and biological activities of the Saharan medicinal plant Limoniastrum feei
Provedor de dados: 60
Autores: Ammar,Ouahab.
ABSTRACT In-silico study was performed to find the pharmacodynamics, toxicity profiles and biological activities of three phytochemicals isolated from Limoniastrum feei (Plumbagenaceae). Online pharmacokinetic tools were used to estimate the potential of Quercetin, kaempferol-3-O-β-D-glucopyranoside (astragalin) and quercitin-7-O-β-D-glucopyranoside as specific drugs. Then the prediction of potential targets of these compounds were investigated using PharmMapper. Auto-Dock 4.0 software was used to investigate the different interactions of these compounds with the targets predicted earlier. The permeability of quercetin was found within the range stated by Lipinski ׳s rule of five. Hematopoietic prostaglandin (PG) D synthase (HPGDS), farnesyl diphosphate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Limoniastrum feei/pharmacokinetics; Limoniastrum feei/biological activity; Quercetin; Astragalin; Quercetin 7; Medicinal plants; Molecular docking.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300606

Aflatoxin M1 in the urine of non-carriers and chronic carriers of hepatitis B virus in Maringa, Brazil
Provedor de dados: 60
Autores: Giolo,Marcel Padovani; Oliveira,Christiane Minervino de; Bertolini,Dennis Armando; Lonardoni,Maria Valdrinez Campana; Gouveia,Matheus Sampaio; Netto,Daisy Pontes; Nixdorf,Suzana Lucy; Machinski Junior,Miguel.
Exposure to aflatoxins (AFs) in the diet may favour the development of hepatocellular carcinoma (HCC) and the acute exacerbation of hepatitis in chronic hepatitis B virus (HBV) carriers. Measurement of biomarkers such as aflatoxin M1 (AFM1), a metabolite of aflatoxin B1 (AFB1), in urine allows for the assessment of populations exposed to aflatoxins. The aim of this study was to investigate the occurrence of aflatoxin M1 in the urine of HBV carrier and non-carrier patients. One group included 43 randomly selected HBV carriers treated at two hospitals in the city of Maringa, Brazil, from March to June 2008. Control group consisted of 29 healthy adult volunteers with anti-HBs positive and HBsAg negative test results. Detection of AFM1 was performed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxins; Biomarkers; Hepatitis B; Hepatocellular carcinoma; Urina/análise toxicológica; Epidemiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300011

Development and characterization of hyaluronic acid-lysine nanoparticles with potential as innovative dermal filling
Provedor de dados: 60
Autores: Carneiro,Jaqueline; Döll-Boscardin,Patrícia Mathias; Fiorin,Bárbara Celânia; Nadal,Jessica Mendes; Farago,Paulo Vitor; Paula,Josiane Padilha de.
ABSTRACT Skin aging causes changes such as wrinkles and flaccidity leading to a large demand for aesthetic procedures, including dermal filling. A key agent in dermal filling is hyaluronic acid (HA), which is a naturally occurring glycosaminoglycan. However, it is a hydrophilic macromolecule that experiences great difficulty in crossing the skin barrier causing most commercial formulations containing it to be injectable, which in turn brings risks since they involve an invasive technique. In that sense, the aim of this study was to develop and characterize nanoparticles obtained from ionic interaction between HA and lysine (Lys) for use as a potential agent of dermal filling for topical application, increasing and improving its applicability and safety. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanotechnology; Skin aging/non-invasive method; Skin aging/treatment/study.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400645

Retrospective evaluation and prediction of clearance and toxicity of high dose methotrexate in childhood acute lymphoblastic leukemia patients
Provedor de dados: 60
Autores: Shan,Yuxia; Gao,Hui; Li,Zhong; Li,Jinghua; Liu,Yang; Li,Lujuan; Zhang,Qi.
To find the predictors of High Dose Methotrexate toxicities in childhood Acute Lymphoblastic Leukemia Patients. This study included 198 Childhood Acute Lymphoblastic Leukemia patients (303 infusions) who were treated with High Dose Methotrexate. Methotrexate levels at different time point were measured by modified enzyme multiplied immunoassay technique assay. The correlation between Methotrexate levels and toxicity was evaluated by Receiver Operating Characteristic curve. When the Methotrexate level at 42 h was lower than 0.76 µmol/L, the sensitivity for predicting thorough clearance at 66 h was 90.78%. When the Methotrexate level at 42 h was higher than1.5 µmol/L, the sensitivity for predicting delayed clearance was 82.17%. When the Methotrexate level at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Childhood acute lymphoblastic leukemia; High dose methotrexate; Toxicity; Leucovorin rescue.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100616

Improvement of physicochemical parameters of acyclovir using cocrystallization approach
Provedor de dados: 60
Autores: Savjani,Jignasa Ketan; Pathak,Chirag.
ABSTRACT Acyclovir is an antiviral drug having potent activity against the virus of herpes family and varicella zoster. Unfortunately, drug suffers very poor oral bioavailability (15-30%). The main objective of present study was to develop acyclovir cocrystals with improved solubility which may result in improvement of bioavailability. Hansen solubility approach was used as a tool to predict the cocrystal formation of a drug with selected coformer. Cocrystals of acyclovir with various coformers were screened in order to enhance their water solubility. Cocrystals of the drug were prepared using various methods like solvent evaporation, wet grinding, and antisolvent addition. Formation of cocrystals by solvent evaporation method was found to be better method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acyclovir/study; Acyclovir/dissolution study; Cocrystal/technique; Hansen solubility parameters; Solvent evaporation method.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400727

Effect of lesimarin against thioacetamide-induced liver cirrhosis in rat
Provedor de dados: 60
Autores: Ra,Sang-Ho; Shin,Ri-Hwa; Ri,Hak-Chol; Ri,Jong-Hui; Ri,Hui-Chol; Ri,Ae-Jong.
Liver cirrhosis is one of chronic liver diseases with high disability and mortality accompanying fibrosis, hepatocyte damage, and liver dysfunction. In this study, the hepatoprotective and the antifibrotic properties of lesimarin(lecithin - silymarin - Artemisia messerschmitiana Besser(AMB) extract complex at 11:3:6 ratio) on rat hepatic fibrosis induced by thioacetamide (TAA) was investigated. Rats were divided into seven groups: control, lesimarin, TAA, TAA+lesimarin, TAA+lecithin, TAA+silymarin, TAA+AMB. Rats were administered with TAA at a dose of 200 mg/kg body weight intraperitoneally twice a week for three months. Lesimarin, lecithin, silymarin and AMB were administered at a dose of 1.0, 1.0, 0.5, 1.0g/kg body weight orally daily for three months,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifibrotic effect; Lesimarin; Thioacetamide; Hepatic fibrosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100598

Association between soluble biomarkers - microbial translocation, inflammation and cardiovascular risk in HIV- infected individuals: a systematic review
Provedor de dados: 60
Autores: Borato,Danielle Cristyane Kalva; Vellosa,José Carlos Rebuglio.
Microbial translocation is associated with the increased risk of cardiovascular disease in HIV-infected individuals. There is scarce information regarding the possible associations between the biomarkers of microbial translocation, inflammation and cardiovascular risk that can be evaluated in clinical laboratories using plasma or serum samples. This systematic review was conducted according to the PRISMA protocol in order to verify the most used soluble biomarkers of microbial translocation, inflammation and cardiovascular risk, as well as possible associations between them, in HIV-infected individuals. A search was performed using the Medline, Scopus and Web of Science databases to identify existing studies regarding the relationship between microbial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial translocation; Inflammation; Cardiovascular risk; HIV; Biomarkers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100614

Byproducts of orange extraction: influence of different treatments in fiber composition and physical and chemical parameters
Provedor de dados: 60
Autores: Andrade,Juliana Maria de Mello; Jong,Erna Vogt de; Henriques,Amélia Teresinha.
In this work we evaluated the variability in fiber content and physical and chemical parameters of byproducts from orange juice extraction. Five different treatments and two drying methods were evaluated. The results indicate that drying by lyophilization was better than that drying in an oven. The pH ranged from approximately 3.47 to 3.96. The variation in moisture values was 9.22% ± 0.02 to 18.48 ± 0.52%. The total dietary fiber content in the resulting flours ranged from 42.44% to 62.74%. The soluble and insoluble dietary fiber contents differed among the samples, ranging from 5.04% to 19.95% for the first fiber type, and 23.96% to 57.70% for the second. In conclusion, three treatments, associated with freeze-drying, showed promising results in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orange/byproducts/evaluation; Orange juice/extraction; Orange/byproducts/fiber content; Fibers; Orange/byproducts/physical-chemical parameters.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300473

Effect of prodigiosin on the alleviation of the intestinal inflammation of weaned rats based on 1H-NMR spectroscopy study and biochemistry indexes
Provedor de dados: 60
Autores: Yang,Peizhou; Wu,Yun; Qian,Jing; Cai,Kezhou; Cao,Lili; Zheng,Zhi; Luo,Shuizhong; Jiang,Shaotong; Zhu,Xingxing.
Weaning results in intestinal dysfunction, mucosal atrophy, transient anorexia, and intestinal barrier defects. In this study, the effect of prodigiosin (PG) on the intestinal inflammation of weaned rats was investigated by using 1H-NMR spectroscopy and biochemistry indexes to regulate the intestinal metabolism. After administration for 14 days, the body mass of the PG group was increased by 1.29- and 1.26-fold compared with those of the control and alcohol groups, respectively, using a dose of 200 µg PG·kg-1 body weight per day. PG increased organic acid content and decreased moisture, pH values, and free ammonia in feces. In addition, PG alleviated the intestinal inflammation of weaned rats. The analysis of 1H-NMR signal peak attribution and the model...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prodigiosin/pharmacokinetics; Proton Magnetic Resonance Spectroscopy/method; Intestinal Absorption/drug effects; Metabolomics/ methods; Rats/weaned; Metabolism/drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100589

A review of nanotechnology with an emphasis on Nanoplex
Provedor de dados: 60
Autores: Kadam,Rupali Nanasaheb; Shendge,Raosaheb Sopanrao; Pande,Vishal Vijay.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanotechnology; Nanoplex/formulation; Nanoplex/characterization; Nanoplex/applications.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200255

Evaluation of triterpenes derivatives in the viability of Leishmania amazonensis and Trichomonas vaginalis
Provedor de dados: 60
Autores: Cargnin,Simone Tasca; Staudt,Andressa Finkler; Menezes,Camila; Azevedo,Ana Paula de; Fialho,Saara Neri; Tasca,Tiana; Teles,Carolina Bioni Garcia; Gnoatto,Simone Baggio.
Trichomonas vaginalis and Leishmania spp. are protozoal species responsible for millions of cases of parasitic diseases worldwide. Considering the potential of natural products and the need for more effective and less toxic alternatives to treat trichomoniasis and leishmaniasis, this study aimed to evaluate the effect of two series of triterpenes derivatives with different modifications at C-3 and C-28 positions of the ursolic acid (UA) and betulinic acid (BA) against trophozoites of Trichomonas vaginalis and promastigotes forms of Leishmania (L.) amazonensis. The compounds modified just at C-3 were the most active. The 3β-acetyl betulinic acid (1b) reduced the trophozoites viability of T. vaginalis at 74%, followed by the 3-oxo ursolic acid and 3-oxo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Betulinic acid; Leishmania amazonensis; Semisynthesis; Trichomonas vaginalis; Ursolic acid.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100596

Enantioselective metabolism of hydroxychloroquine employing rats and mice hepatic microsomes
Provedor de dados: 60
Autores: Cardoso,Carmem Dickow; Bonato,Pierina Sueli.
Hydroxychloroquine (HCQ) is an important chiral drug used, mainly, in the treatment of rheumatoid arthritis, systemic lupus erythematosus and malaria, and whose pharmacokinetic and pharmacodynamic properties look to be stereoselective. Respecting the pharmacokinetic properties, some previous studies indicate that the stereoselectivity could express itself in the processes of metabolism, distribution and excretion and that the stereoselective metabolism looks to be a function of the studied species. So, the in vitro metabolism of HCQ was investigated using hepatic microsomes of rats and mice. The microsomal fraction of livers of Wistar rats and Balb-C mice was separated by ultracentrifugation and 500 μL were incubated for 180 minutes with 10 μL of racemic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxychloroquine; Stereoselective metabolism/in vitro study; High performance liquid chromatography; Capillary electrophoresis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400008

Development and application of a portable instrument for drugs analysis in pharmaceutical preparations
Provedor de dados: 60
Autores: Ribeiro,Paulo Roberto da Silva; Pezza,Helena Redigolo; Pezza,Leonardo; Lima,Liliane Spazzapam; Tubino,Matthieu.
This article describes the application and performance of an inexpensive, simple and portable device for colorimetric quantitative determination of drugs in pharmaceutical preparations. The sensor is a light detector resistor (LDR) incorporated into a black PTFE cell and coupled to a low-cost multimeter (Ohmmeter). Quantitative studies were performed with captopril/p-chloranil/H2O2 and methyldopa/ammonium molybdate systems. Calibration curves were obtained by plotting the electrical resistance of the LDR against the concentration of the colored species in the ranges 1.84 × 10-4 to 1.29 × 10-3mol L-1 and 5.04 × 10-4 to 2.52 × 10-3 mol L-1 for captopril/p-chloranil/H2O2 and methyldopa/ammonium molybdate systems, respectively, exhibiting good coefficients of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optical sensor/application/colorimetric analysis; Instrumentation/drugs dosage; Drugs/quantitative analysis; Drugs/dosage; Pharmaceutical formulations/quality control.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300699

Cost-consequence analysis of Pharmaceutical Care program for systemic arterial hypertension in the public health system in Brazil
Provedor de dados: 60
Autores: Cazarim,Maurílio de Souza; Nunes,Altacílio Aparecido; Pereira,Leonardo Régis Leira.
ABSTRACT In Brazil, 80% of hypertensive patients have no blood pressure controlled, this fact has caused severe financial consequences for the public health system (PHS) and the Pharmaceutical Care (PC) has emerged as an effective alternative. The aim of this study was to analyze the costs and outcomes of systemic arterial hypertension (SAH) for conventional assistance compared to assistance with PC in the PHS. This is a pharmacoeconomic study with cost-consequence analysis nested to clinical trial. Hypertensives patients were followed-up from 2006 to 2012. During 2009 they were assisted by the PC program in Ribeirão Preto-SP, Brazil. Clinical indicators, systolic and diastolic blood pressure (SBP and DBP), triglycerides, total cholesterol (TC) and its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical economics; Pharmaceutical services; Health Expenditures; Hypertension; Pharmaceutical care; Costs and cost analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300620

Quality parameters of some Brazilian panettones
Provedor de dados: 60
Autores: Valcárcel-Yamani,Beatriz; Lannes,Suzana Caetano da Silva.
In panettone, physical and chemical properties can determine the quality attributes in addition to consumer acceptability. The aim of this work was to assess the quality attributes of nine commercial samples of Brazilian panettone. Samples were characterized according to their physicochemical (moisture and color) aspects, quality attributes (specific volume, density and texture) and image analysis. The results of the statistical analysis (ANOVA and Tukey) showed significant differences (p&lt;0.05) among the samples. Differences were observed in the specific volume and density. Samples showed moisture values between 22.83% and 26.86%. The texture analysis showed greater variation in values of crumb firmness (from 2.14 N to 7.55 N). Significant differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panettone/physical and chemical properties; Panettone/qualitative analysis; Panettone/texture; Panettone/color; Panettone/image analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300012

Assessment of fibrosis and vascularization of bone marrow stroma of chronic myeloid Leukemia patients treated with imatinib mesylate and their relationship with the cytogenetic response
Provedor de dados: 60
Autores: Jesus,Caroline Regina de; I-Ching,Lee; Neiva,Teresinha de Jesus Carvalho; Vituri,Cidônia de Lourdes.
Chronic Myeloid Leukemia (CML) is a myeloproliferative disease characterized by the presence of the Philadelphia chromosome (translocation between chromosomes 9 and 22), resulting in the formation of the hybrid BCR-ABL protein. Currently, the treatment of CML patients is performed with imatinib mesylate (IM), which promotes the elimination of leukemic cells by inhibiting the kinase activity of BCR-ABL. This study evaluated the effectiveness of IM by monitoring 22 CML patients in a chronic phase treated at the CEPON/SC with IM for a minimum follow-up period of two years. Cytogenetic Response (CR) and bone marrow biopsies (BMB) were evaluated before and after IM treatment. BMB were evaluated by detection of reticulin degree and vascularization. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic myeloid leukemia/treatment; Imatinib mesylate/effectiveness; Imatinib mesylate/leukemia treatment; Bone marrow/biopsies; Fibrosis; Vascularization.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200012

Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity
Provedor de dados: 60
Autores: Silvestre,Marialice Pinto Coelho; Silva,Mauro Ramalho; Silva,Viviane Dias Medeiros; Souza,Mariana Wanessa Santana de; Lopes Junior,Carlos de Oliveira; Afonso,Wendel de Oliveira.
The aim of this study was to prepare enzymatic hydrolysates from whey protein concentrate with a nutritionally adequate peptide profile and the ability to inhibit angiotensin-converting enzyme (ACE) activity. The effects of the type of enzyme used (pancreatin or papain), the enzyme:substrate ratio (E:S ratio=0.5:100, 1:100, 2:100 and 3:100) and the use of ultrafiltration (UF) were investigated. The fractionation of peptides was performed by size-exclusion-HPLC, and the quantification of the components of the chromatographic fractions was carried out by a rapid Corrected Fraction Area method. The ACE inhibitory activity (ACE-IA) was determined by Reverse Phase-HPLC. All parameters tested affected both the peptide profile and the ACE-IA. The best peptide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whey proteins; Protein hydrolysates; Peptide profile; Inhibitory activity; Angiotensin-converting enzyme.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400019

Categorization of the main descriptors of different ampicillin crystal habits
Provedor de dados: 60
Autores: Flórez-Acosta,Oscar; Tobón-Zapata,Gloria; Valencia-Velasquez,Jaime.
With the purpose of enabling the analysis by digital methods of particles of multisource pharmaceutical raw materials, this study analyzed different crystal habits of ampicillin particles, by grouping the external shapes obtained from 3 different solvents (acetonitrile, ethanol, and methanol), thereby reducing the number of descriptors necessary to adequately represent each shape. For this purpose, a selection of morphological descriptors was used including: circularity, roughness, roundness, compactness, aspect ratio, effective diameter, solidity, convexity, fractal dimension, and 10 Complex Fourier descriptors. These measures cover highly diverse morphological properties and define the crystal habit of a particle. Principal Component Analysis (PCA) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multisource solids; Morphological descriptors; Fourier descriptors; Crystal habits; Multivariate analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400009

Safety assessment of cosmetic products, with emphasis on the ocular area: regulatory aspects and validation processes
Provedor de dados: 60
Autores: Cancian,Maythê de Lima; Leonardi,Mauricio Saadi; Mosca,Mariane Martins; Guerra,Lucas Offenbecker; Hengeltraub,Simone Fanan; Leonardi,Gislaine Ricci.
Before marketing a cosmetic product, a series of biological assays, such as ocular irritation tests, must be conducted in order to prove that the product is safe. However, a few scientific articles mention the discussion and evolution of cosmetic products testing performed in the eyes area. The aim of this study was to review the available literature on the evolution of tests carried out with cosmetics, in the ocular area, as well as to describe the methodologies that have been used and that are currently accepted. In Brazil, tests performed on animals are still allowed. However, the international laws strongly recommend the use of alternative methods for evaluating the risk of cosmetic ingredients and products. Regulatory requirements involving the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eyes/cosmetics use; Cosmetics/safety assessment; Ocular irritation/tests; Ocular area/safety use of cosmetics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400667

Seroprevalence of HBV and HCV markers among young adult males in the Air Force in Florianópolis, South Brazil
Provedor de dados: 60
Autores: Passos,Ana Maria; Strazer Neto,Miguel; Treitinger,Aricio; Spada,Celso.
We investigated the prevalence of hepatitis B virus (HBV) and hepatitis C virus (HCV) serological markers of infection in young adults from the metropolitan region of Florianópolis who were conscripts of the Air Base of Florianópolis in the state of Santa Catarina, Brazil. A population-based cross-sectional seroprevalence study was conducted with 371 young males during a one year period starting in June 2009. Demographic characteristics, socio-economic characteristics and possible risk factors to HBV and HCV were assessed. Blood samples were analyzed for HBsAg, anti-HBc, anti-HCV and anti-HBs through automated microparticle enzymatic immunoassays (Abbott®, AxSYM System, Wiesbaden, Germany). None of the participants showed positivity to HBsAg or anti-HCV....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HBV; HCV; Hepatitis B; Hepatitis C; Seroprevalence.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200009

Clinical efficacy of resveratrol as an adjuvant with meloxican in the treatment of knee osteoarthritis patients: A double-blind, randomised, placebo-controlled trial
Provedor de dados: 60
Autores: Marouf,Bushra Hassan; Hussain,Saad Abdulrahman; Ali,Ziyad Serdar; Ahmmad,Runj Simko.
The present study aimed to evaluate the effect of the adjuvant use of resveratrol with meloxicam on the clinical scores of knee OA patients. This was a double-blind placebo-controlled randomised trial involving 100 patients with knee osteoarthritis performed at the Shar Teaching Hospital, Sulaimani General Hospital and Specialised Rheumatology Center, Sulaimani City from December 2016 to September 2017. The efficacy of the treatment was evaluated by measuring the changes from baseline in the KOOS score, WOMAC index, and VAS-100 score after 90 days of treatment. Resveratrol significantly improves the knee OA pain and associated symptoms compared with placebo, and both clinical scores were found to be eligible for following treatment outcomes. In conclusion,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resveratrol/effects; Meloxicam/effects; Knee OA; KOOS; WOMAC.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400627

Determination of phytoconstituents of n-hexane extract of leaves of Morus nigra and evaluation of their effects on biochemical and histopathological parameters in paracetamol intoxicated mice liver
Provedor de dados: 60
Autores: Mallhi,Tauqeer Hussain; Qadir,Muhammad Imran; Khan,Yusra Habib.
In-vitro investigation of Morus nigra L. (Moraceae) has demonstrated the evidence of several antioxidant compounds. Current study was aimed to determine the phytoconstituents and hepatoprotective potential of n-hexane extract of Morus nigra. Swiss albino mice were divided into five groups: normal control, paracetamol control, silymarin control, 250 mg/kg p.o. extract for 7 days plus paracetamol 3 h later, 500 mg/kg p.o. extract for 7 days plus paracetamol 3 h later. Serum levels of liver enzymes and total bilirubin were assessed and compared between the groups by using one-way ANOVA to confirm hepatoprotective activity. HPLC analysis revealed the presence quercetin, oleanolic acid, luteolin, apigenin, vitamin C and kuwanon C. n-hexane extract of Morus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moraceae; HPLC; Quercetin; Morus nigra/antioxidant; Morus nigra/extract/hepatoprotective.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300631

Impact of a medication therapy management service offered to patients in treatment of breast cancer
Provedor de dados: 60
Autores: Amaral,Pedro Augusto do; Mendonça,Simone de Araújo Medina; Oliveira,Djenane Ramalho de; Peloso,Leonardo José; Pedroso,Reginaldo dos Santos; Ribeiro,Maria Ângela.
ABSTRACT Cancer has high morbidity and mortality rates related to medication use and produce a costly impact in health care. Thus, patients require constant monitoring and proper coordination of care between different professionals. This study aimed to evaluate the impact generated by a Medication Therapy Management service (MTM) offered to patients with breast cancer in use of polypharmacy. Observational, exploratory, descriptive and retrospective study of a MTM service that included 93 patients. Sociodemographic and clinical data related to pharmacotherapy and the processes associated with the systematization of the service were collected and analyzed. Patients were followed-up by the MTM service on average for 18 months (±4.31) and 185 drug-related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medication therapy management services; Outcomes Pharmaceutical care; Oncology; Breast Cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200626

Stability of antioxidant vitamins in commercial vitamin supplements
Provedor de dados: 60
Autores: Abe-Matsumoto,Lucile Tiemi; Sampaio,Geni Rodrigues; Bastos,Deborah Helena Markowicz.
The stability of vitamins A, E, and C was determined in 12 brands of vitamin supplements over a 12-month storage period. The variations in concentrations of these vitamins across three different batches of five brands were measured. Vitamins A and E was determined by HPLC method, and vitamin C was measured by using potentiometric titration. All samples for stability studies were maintained at room temperature and protected from light. Measurements were carried out in the first semester of the expiration date and then every six months up to 12 months of storage. After this period, only one sample showed no significant decrease in vitamin A and E concentrations in relation to the concentrations measured at the beginning of the study. The concentration of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Supplements/stability Vitamins A; E C/concentration.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400623

Development and evaluation of sustained release losartan potassium matrix tablet using kollidon SR as release retardant
Provedor de dados: 60
Autores: Sarwar,Shahid; Hossain,Mohammad Salim.
The present study was undertaken to develop sustained release (SR) matrix tablets of losartan potassium, an angiotensin-II antagonist for the treatment of hypertension. The tablets were prepared by direct compression method, along with Kollidon SR as release retardant polymer. The amount of losartan potassium remains fixed (100 mg) for all the three formulations whereas the amounts of Kollidon SR were 250 mg, 225 mg, and 200 mg for F-1, F-2, and F-3 respectively. The evaluation involves three stages: the micromeritic properties evaluation of granules, physical property studies of tablets, and in-vitro release kinetics studies. The USP apparatus type II was selected to perform the dissolution test, and the dissolution medium was 900 mL phosphate buffer pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Losartan potassium/sustained release; Losartan potassium/development; Losartan potassium/sustained release; Losartan potassium/evaluation; Tablets/direct compression; Kollidon SR./use; Kollidon SR./release retardant.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400005

Hair fiber characteristics and methods to evaluate hair physical and mechanical properties
Provedor de dados: 60
Autores: Velasco,Maria Valéria Robles; Dias,Tania Cristina de Sá; Freitas,Anderson Zanardi de; Júnior,Nilson Dias Vieira; Pinto,Claudinéia Aparecida Sales de Oliveira; Kaneko,Telma Mary; Baby,André Rolim.
The hair thread is a natural fiber formed by keratin, a protein containing high concentration of sulfur coming from the amino acid cystine. The main physical proprieties of the hair depend mostly on its geometry; the physical and mechanical properties of hair involve characteristics to improve: elasticity, smoothness, volume, shine, and softness due to both the significant adherence of the cuticle scales and the movement control (malleability), as well as the easiness of combing, since they reduce the fibers static electricity. The evaluation of these effects on hair may be carried out by several methods, as: optical and electron microscopy, mechanical resistance measuring, shine evaluation and optical coherence tomography (OCT).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hair/properties; Hair/evaluation; Hair fibers/properties; Hair fibers/evaluation; Hair fibers/characteristics; Optical coherence tomography/analytical application; Optical microscopy/analytical application; Electron microscopy/analytical application.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100019

Comparative study on morpho-anatomy of leaf, stem and root of Boerhaavia diffusa L. (Nyctaginaceae) and its adulterant plants
Provedor de dados: 60
Autores: Patil,Vidya Shivram; Rajput,Kishore Shankarsinh; Malpathak,Nutan Padmnabha.
ABSTRACT Punarnava (Boerhaavia diffusa L.- Nyctaginaceae) is a promising drug to rejuvenate new cells in the body. It is well known in Ayurvedic medicine and locally called Tambadivasu. Superficially it is similar to other species of Boerhaavia and species of Trianthema and Sesuvium. Due to the minute morphological differences, the above plants are erroneously used in medicine as Punarnava, and at times on purpose as an adulterant. Therefore, it is necessary to highlight the anatomical features of Punarnava for proper identification of the medicinal plant species for local people and for scientific research. Due to the ambiguity in local names and similar apparent appearance, market samples of Punarnava are often adulterated with various species of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants/comparative study; Medicinal plants/Scanning Electron Microscopy; Ayurvedic medicine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300433

Isolation and preliminary evaluation of Mulva Neglecta mucilage: a novel tablet binder
Provedor de dados: 60
Autores: Rahim,Haroon; Sadiq,Abdul; Khan,Shahzeb; Chishti,Kamran Ahmad; Amin,Fazli; Khan,Mir Azam; Abbas,Sudhair.
ABSTRACT The aim of this study was to evaluate binding potential of Mulva neglecta mucilage (MNM) with subsequent comparison to PVP K30. Eight batches of Diclofenac sodium tablets were prepared by wet granulation technique keeping different concentrations (4, 6, 8 &amp; 10% w/w) of Mulva neglecta mucilage (extracted from leaves of Mulva neglecta) and PVP K30 as standard binder. The granules of formulated batches showed bulk density (g/mL) 0.49 ± 0.00 to 0.57 ± 0.00, tapped density (g/mL) 0.59 ± 0.01 to 0.70 ± 0.01, Carr's index 09.27 ± 0.95 to 19.65 ± 0.59, Hausner's ratio 1.12 ± 0.00 to 1.24 ± 0.01 and angle of repose 30.37 ± 2.90 °C to 36.86 ± 0.94 °C. Tablets were compressed to hardness 7.50 to 7.95 kg/cm2. The tablets showed 0.39 ± 0.02 to 0.39 ± 0.01%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mulva neglecta/mucilage; Mulva neglecta/use as binder; Binders; Diclofenac sodium/tablets/drug release.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100022

Synaptic vesicle cycling is not impaired in a glutamatergic and a cholinergic synapse that exhibit deficits in acidification and filling
Provedor de dados: 60
Autores: Abreu,Bento João; Leite,Luciana Ferreira; Oliveira,Débora Lopes; Amaral,Ernani.
The purpose of the present work was to investigate synaptic vesicle trafficking when vesicles exhibit alterations in filling and acidification in two different synapses: a cholinergic frog neuromuscular junction and a glutamatergic ribbon-type nerve terminal in the retina. These synapses display remarkable structural and functional differences, and the mechanisms regulating synaptic vesicle cycling might also differ between them. The lipophilic styryl dye FM1-43 was used to monitor vesicle trafficking. Both preparations were exposed to pharmacological agents that collapse ΔpH (NH4Cl and methylamine) or the whole ΔµH+ (bafilomycin), a necessary situation to provide the driving force for neurotransmitter accumulation into synaptic vesicles. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synaptic vesicle; Acidification; Lipophilic styryl dye/use; Neuromuscular junction; Bipolar cell; Retina.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100017

Physicochemical characterization and solubility enhancement studies of allopurinol solid dispersions
Provedor de dados: 60
Autores: Changdeo,Jagdale Swati; Vinod,Musale; Shankar,Kuchekar Bhanudas; Rajaram,Chabukswar Anuruddha.
Allopurinol is a commonly used drug in the treatment of chronic gout or hyperuricaemia associated with treatment of diuretic conditions. One of the major problems with the drug is that it is practically insoluble in water, which results in poor bioavailability after oral administration. In the present study, solid dispersions of allopurinol were prepared by solvent evaporation, kneading method, co-precipitation method, co-grinding method and closed melting methods to increase its water solubility. Hydrophilic carriers such as polyvinylpyrrolidone, polyethylene glycol 6000 were used in the ratio of 1:1, 1:2 and 1:4 (drug to carrier ratio). The aqueous solubility of allopurinol was favored by the presence of both polymers. These new formulations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allopurinol; Polyethylene glycol 6000; Solid dispersions; Closed melting method; Dissolution enhancement.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300009

Development of methodologies for dimethylaminoethanol glycolate assay in association with sunscreens in dermocosmetic formulation
Provedor de dados: 60
Autores: Deccache,Daniela Soares; Santos,Elisabete Pereira dos; Cabral,Lúcio Mendes; Rodrigues,Carlos Rangel; Sousa,Valéria Pereira de.
DMAE glycolate (DG) and sunscreens have been used associated in anti-aging dermocosmetic formulations. Despite extensive use of these substances, methods for quantification of DG as raw material and in cosmetic formulations, especially when associated, are not described in the literature. RP-HPLC and non-aqueous titration methods, with determination potentiometric end-point (PT), were developed and validated for rapid assay of DG as raw material and in a topic emulsion in association with sunscreens. Both methods are simple, selective, linear, accurate and precise. The PT method was chosen for stability study of DG in the formulation developed. The proposed formulation presented good stability performance as regards aspect, pH, apparent viscosity, and SPF,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DMAE glycolate; Anti-aging dermocosmetic formulations/quantitative analysis; High Performance Liquid Chromatography-RP/quantitative analysis; Non-aqueous potentiometric titration/quantitative analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400012

Potentially inappropriate medication use among institutionalized elderly individuals in southeastern Brazil
Provedor de dados: 60
Autores: Pinto,Mauro Cunha Xavier; Malaquias,Dalila Pinto; Ferré,Felipe; Pinheiro,Marcos Luciano Pimenta.
In recent decades, the elderly population in Brazil has grown rapidly, as has concern for the health of this population. Institutionalization in nursing homes has appeared as an alternative form of health care for frail elderly that live alone. The present study evaluated the pharmacotherapy and inappropriate drug prescriptions for institutionalized elderly patients living in long-term institutions in southeastern Brazil. This research was conducted at five institutions with a total sample of 151 individuals aged at least 60 years. Databases were used to identify drug interactions, defined daily dose and inappropriate prescriptions. The prevalence of drug intake among the elderly was 95.36%, and there were an average of 3.31 ± 1.80 drug prescriptions per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacoepidemiology; Drug interactions; Beers criteria; Elderly/inappropriate use of medicines; Medicines/inappropriate use.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400010

Chemical composition, phytotoxic potential, biological activities and antioxidant properties of Myrcia hatschbachii D. Legrand essential oil
Provedor de dados: 60
Autores: Gatto,Larissa Junqueira; Fabri,Natasha Tiemi; Souza,Angela Maria de; Fonseca,Natasha Stopinski Triaquim da; Furusho,Alethéia dos Santos; Miguel,Obdulio Gomes; Dias,Josiane de Fatima Gaspari; Zanin,Sandra Maria Warumby; Miguel,Marilis Dallarmi.
Myrcia hatschbachii D. Legrand (Myrtaceae) is a native and endemic species from Brazil. This study investigated the essential oil composition, phytotoxic potential, in vitro toxicity, antioxidant properties and antibacterial activity of species. Chromatographic analysis of essential oil identified trans-calamanene, (E)-caryophyllene and spathulenol as major components. Antioxidant capacity was determined by the DPPH• scavenging method and phosphomolybdenum complex formation assay. Antibacterial activity was evaluated using the Minimal Inhibitory Concentration test, demonstrating that the essential oil was active toward Enterococcus faecalis and Staphylococcus aureus. Phytotoxic activity of essential oil was analyzed by testing interference on germination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oil; Phytotoxic; Antioxidant; Antibacterial; Hemolysis; Artemia salina.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100560

Guideline values and human risk assessment for the presence of anti-inflammatory drugs remaining in drinking water after lab scale treatment
Provedor de dados: 60
Autores: Pais,Mariana Castello Novo; Nascimento,Elizabeth de Souza.
Abstract This study aimed to determine whether the anti-inflammatory drugs that are most commonly consumed in Brazil, including diclofenac, ketoprofen, naproxen, indomethacin, ibuprofen and acetaminophen, are present in drinking water and to derive guideline values to characterize the human risk. These pharmaceuticals were quantified in surface waters by LC-MS/MS with solid phase extraction, both before and after conventional treatment on a laboratory scale, using a jar test assay. The methods used to quantify these drugs showed good results: the chromatographic analysis obtained correlation coefficients between 0.9952 and 0.9991, with limits of quantification of 0.5 ng.mL-1 - 50 ng.mL-1 and precision standard deviations (0.08 - 2.08). Only ketoprofen and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emerging contaminant; LC-MS/MS; Human risk assessment; Pharmaceuticals in drinking water.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100624

Relationship between oxidative stress and diabetic osteopenia in premenopausal rats
Provedor de dados: 60
Autores: Duarte,Valéria Morgiana Gualberto; Rodrigues,Alanna de Sousa; Rezende,Luciana Augusto de; Ramos,Ana Maria de Oliveira; Souza,Rodrigo Medeiros de; Freire Neto,Francisco Paulo; Medeiros,Aldo da Cunha; Brandão Neto,José; Almeida,Maria das Graças; Rezende,Adriana Augusto de.
The relationship between lipid peroxidation, antioxidant defense and diabetic osteopenia remains unclear. This study evaluated the relationship among lipid peroxidation index, antioxidant defense parameters and bone metabolism in a premenopausal diabetic model using measures including thiobarbituric acid-reactive substances concentration (TBARS) and reduced glutathione (GSH) content in brain homogenates, histomorphometric analysis, biomechanical testing and bone mineral density (BMD). Female Wistar rats with regular estrous cycle were divided into two groups: Group 1: control rats (n = 15) and Group 2: diabetic rats (n = 15). Diabetes was induced by alloxan and confirmed by glycemia &amp;gt;250 mg/dL. The lipid peroxidation index, measured by TBARS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Lipid peroxidation; Diabetes mellitus; Diabetic osteopenia/experimental study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300018

Clinical and economic aspects of the use of rituximab in non-Hodgkin's lymphoma
Provedor de dados: 60
Autores: Figueirêdo,Camila Bezerra Melo; Souza,Joelma Rodrigues de; Soares,Daniel Handerson Galindo; Silva,Caio Cesar de Andrade Rodrigues; Lorena,Virginia Maria Barros de.
Non-Hodgkin's lymphoma (NHL) consists of a group of neoplasias involving mainly B cells and represents 90% of all lymphomas. The current available therapy is based on chemotherapy associated with the monoclonal antibody rituximab (Mab Thera(r)), which targets the CD20 protein, present in over 80% of NHL mature B cells. Recent clinical reports show a preference for combining the benefits of immunotherapy and adjuvant chemotherapy, thus generating safe and effective alternative treatments. The current review aimed at evaluating various aspects related to the use of rituximab for NHL, highlighting the possible inhibitory mechanisms of cell proliferation, the achieved clinical results, and the expected clinical and economic outcomes of treatments. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-Hodgkin's lymphoma; Antineoplastic combined chemotherapy/protocols; Adjuvant chemotherapy; Rituximab/application/Non-Hodgkin's lymphoma.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300445

Development of dissolution test method for a telmisartan/amlodipine besylate combination using synchronous derivative spectrofluorimetry
Provedor de dados: 60
Autores: Anumolu,Panikumar Durga; Neeli,Sirisha; Anuganti,Haripriya; Ranganatham,Sathesh Babu Puvvadi; Satya,Subrahmanyam Chavali Venkata.
The dissolution process is considered an important in vitro tool to evaluate product quality and drug release behavior. Single dissolution methods for the analysis of combined dosage forms are preferred to simplify quality control testing. The objective of the present work was to develop and validate a single dissolution test for a telmisartan (TEL) and amlodipine besylate (AML) combined tablet dosage form. The sink conditions, stability and specificity of both drugs in different dissolution media were tested to choose a discriminatory dissolution method, which uses an USP type-II apparatus with a paddle rotating at 75 rpm, with 900 mL of simulated gastric fluid (SGF without enzymes) as the dissolution medium. This dissolution methodology provided good...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Combined dosage forms/quality control; Dissolution test/combined dosage forms; Telmisartan; Amlodipine besylate; Spectrofluorimetry/quantification analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200329

Pullulan as a stabilizer agent of polymeric nanocapsules for drug delivery
Provedor de dados: 60
Autores: Chassot,Janaíne Micheli; Ferreira,Luana Mota; Gomes,Felipe Pereira; Silva,Cristiane de Bona da; Tasso,Leandro; Cruz,Letícia.
ABSTRACT Polymeric stabilizers have received attention in the preparation of nanostructured systems due to their ability to enhance formulation stability. Considering this, the objective of this work was to prepare poly(ε-caprolactone) nanocapsules using the pullulan as a polymeric stabilizer. The nanocapsules were prepared using the interfacial deposition method of preformed polymers and they were characterized in terms of pH, average diameter, polydispersity index, zeta potential, beclomethasone dipropionate content, encapsulation efficiency, photostability and drug release profiles. The formulations showed physicochemical characteristics consistent with nanocarriers for drug delivery such as: average diameter lower than 270 nm, polydispersity indexes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pullulan; Nanocapsules/diffusion technique; Pullulan/polymeric stabilizer; Biocompatible polymers; Beclomethasone dipropionate/nanocapsules.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400735

Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
Provedor de dados: 60
Autores: Razzaq,Syed Naeem; Ashfaq,Muhammad; Khan,Islam Ullah; Mariam,Irfana; Razzaq,Syed Saleem; Mustafa,Ghulam; Zubair,Muhammad.
Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gatifloxacin; Dexamethasone; High performance liquid chromatography; Validation; Plasma; Degradation products.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100607

Stability-indicating RP-HPLC method for determination of beclomethasone dipropionate in nanocapsule suspensions
Provedor de dados: 60
Autores: Chassot,Janaíne Micheli; Ferreira,Luana Mota; Gomes,Felipe Pereira; Cruz,Letícia; Tasso,Leandro.
abstract A simple stability-indicating RP-HPLC/UV method was validated for determination of beclomethasone dipropionate (BD) in nanocapsule suspensions. Chromatographic conditions consisted of a RP C18column (250 mm x 4.60 mm, 5 µm, 110 Å), using methanol and water (85:15 v/v) as mobile phase at 1.0 mL/min with UV detection at 254 nm. The calibration curve was found to be linear in the concentration range of 5.0-25.0 µg/mL with a correlation coefficient &gt; 0.999. Precision was demonstrated by a relative standard deviation lower than 2.0%. Accuracy was assessed by the recovery test of BD from nanocapsules (98.03% to 100.35%). Specificity showed no interference from the components of nanocapsules or from the degradation products derived from acid, basic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography/reverse phase/quantitative analysis; Beclomethasone dipropionate/determination; Nanocapsule/suspensions; Nanoparticles.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400803

Balneotherapy is a potential risk factor for Pseudomonas aeruginosa colonization
Provedor de dados: 60
Autores: Deutsch,Gabriela; Bokehi,Luciana Castilho; Silva,Ana Elisa Gonçalves Pereira Rodrigues; Guimarães Junior,Luiz Macieira; Rodrigues,Rogério; Esper,Luciana Maria Ramires; Gonzalez,Alice Gonçalves Martins; Paula,Geraldo Renato de; Castilho,Selma Rodrigues de; Teixeira,Lenise Arneiro.
ABSTRACT The practice of immersion in burn patient has been abandoned in many parts of the world but in Brazil it is still common. The aim of this study was to ascertain if balneotherapy is a risk factor for Pseudomonas aeruginosa colonization in thermally injured patients. Eighteen patients from a Burn Center were studied for 14 weeks for Pseudomonas aeruginosa. Samples were collected by swabbing the exudate of wounds, before and after giving bath to the patients and from balneotherapy table. Pulsed-field gel electrophoresis was used to determine bacterial genetic relatedness. Thirty-seven P. aeruginosa isolates were detected from 292 swabs collected from patients' burn surface area and from the balneotherapy table. Profile analysis of P. aeruginosa DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa/colonization; Burn patients; Balneotherapy; PFGE.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100014

In vitro anticancer activity of microbial isolates from diverse habitats
Provedor de dados: 60
Autores: Thomas,Angel Treasa; Rao,Josyula Venkata; Subrahmanyam,Volety Mallikarjuna; Chandrashekhar,Hariharapura Raghu; Maliyakkal,Naseer; Kisan,Tukaram Kedar; Joseph,Alex; Udupa,Nayanabhirama.
Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticancer; Cytotoxicity; Flow cytometry; Microbial extracts; MTT assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200009

The role of selenium in insulin resistance
Provedor de dados: 60
Autores: Fontenelle,Larissa Cristina; Feitosa,Mayara Monte; Morais,Jennifer Beatriz Silva; Severo,Juliana Soares; Freitas,Taynáh Emannuelle Coelho de; Beserra,Jéssica Batista; Henriques,Gilberto Simeone; Marreiro,Dilina do Nascimento.
ABSTRACT In recent years, there has been growing interest in clarifying the pathogenesis of some chronic diseases, such as obesity and type 2 diabetes mellitus. Metabolic alterations in these diseases are characterized by chronic hyperglycemia and insulin resistance. Studies have demonstrated the participation of minerals in the pathogenesis of insulin resistance, more specifically their involvement in the synthesis and regulation of insulin. Selenium is an anti-inflammatory and antioxidant micronutrient that is essential for the activity of selenoproteins. Two selenoproteins (glutathione peroxidase and selenoprotein P) are known to be involved in the insulin signaling pathway. The aim of this review is to provide an update on the role of selenium in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Selenium/insulin resistance; Insulin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100401

Use of Curcuma longa in cosmetics: extraction of curcuminoid pigments, development of formulations, and in vitro skin permeation studies
Provedor de dados: 60
Autores: Gonçalves,Gisele Mara Silva; Silva,Gustavo Henrique da; Barros,Pedro Paulo; Srebernich,Silvana Mariana; Shiraishi,Cecilia Toyoko Cavalcanti; Camargos,Victória Rodrigues de; Lasca,Thais Barbiero.
Curcuma longais a ginger family aromatic herb (Zingiberaceae) whose rhizomes contain curcuminoid pigments, including curcumin, a compound known for its anti-inflammatory effects. The objective of this study was to obtain curcuminoid-rich extracts, develop topical formulations thereof, and assess the stability and skin permeation of these formulations. Curcuma longa extracts were obtained and used to develop formulations. Skin permeation studies were conducted in a modified Franz diffusion cell system, and skin retention of curcuminoid pigments was quantified in pig ear membrane. Prepared urea-containing gel-cream formulations were unstable, whereas all others had satisfactory stability and pseudoplastic rheological behavior. The amount of curcuminoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcuma longa/pharmacognosy; Curcumin/use/topical anti-inflammatory; Cosmetics/use of natural products; Curcuminoid pigments; Skin penetration; Natural products/use in cosmetics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400885

Characterization of mucoadhesive nasal gels containing midazolam hydrochloride prepared from Linum usitatissimum L. mucilage
Provedor de dados: 60
Autores: Basu,Shyamoshree; Bandyopadhyay,Amal Kumar.
Nasal drug delivery systems prepared from natural materials are gaining importance in the field of pharmaceutical technology. Mucilage isolated from Linum usitatissimum L. (LUM) seeds was reported to be an effective natural mucoadhesive agent. The present study deals with a comparison of various characteristics of nasal gels containing midazolam hydrochloride (HCl) prepared from mucoadhesive agent extracted from Linum usitatissimum L. seeds and synthetic polymers like HPMC and Carbopol 934P in terms of texture profile analysis, mucoadhesive strength, and in vivo drug absorption profiles. It was observed that gels formulated with the natural mucilage showed better results than the synthetic gels in all aspects like hardness, adhesiveness, cohesiveness and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nasal drug/delivery systems; Midazolam hydrochloride/bioavailability; Linum usitatissimum L./pharmacognosy; Linum usitatissimum L./mucoadhesive agent; Natural mucilage/nasal gel.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400019

Development of O/W emulsions containing Euterpe oleracea extract and evaluation of photoprotective efficacy
Provedor de dados: 60
Autores: Daher,Cláudia Cecilio; Fontes,Ipojucan Silva; Rodrigues,Rayllan de Oliveira; Damasceno,Gabriel Azevedo de Brito; Soares,Daiane dos Santos; Aragão,Cícero Flávio Soares; Gomes,Ana Paula Barreto; Ferrari,Márcio.
Euterpe oleraceaMart. is a palm tree popularly known as açai, which is primarily found in northern Brazil. The açai's fruits contain anthocyanins, a class of polyphenols to which antioxidant properties have been attributed. The aim of this work was to develop O/W sunscreens emulsions containing açai glycolic extract (AGE) and to evaluate both their physical stability and photoprotective efficacy. Emulsions containing AGE and sunscreens were formulated using different types and concentrations of polymeric surfactant (acrylates/C 10-30 alkyl acrylate crosspolymer and sodium polyacrylate). The influence of two rheology modifiers (polyacrylamide (and) C13-14/isoparaffin (and) Laureth-7 and Carbomer) on the stability was also investigated. Physical stability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: O/W emulsion/development; Euterpe oleracea/pharmacognosy; Euterpe oleracea/glycolic extract/photoprotective efficacy; Sunscreens/development; Sun Protection Factor.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300639

Gastric-resistant isoniazid pellets reduced degradation of rifampicin in acidic medium
Provedor de dados: 60
Autores: Freire,Fátima Duarte; Câmara,Manuela Bernardo; Dantas,Monique Gomes; Aragão,Cícero Flávio Soares; Lima e Moura,Túlio Flávio Accioly de; Rafﬁn,Fernanda Nervo.
Isoniazid and rifampicin are considered the first-line medication for preventing and treating tuberculosis. Rifampicin is degraded in the stomach acidic environment, especially when combined with isoniazid, factor contributing to treatment failure. In this study, gastric-resistant isoniazid pellets were obtained to physical contact of this drug with rifampicin and to bypass the stomach´s acidic environment. The pellets were fabricated using the extrusion-spheronization technique. The coating process was conducted in a fluid spray coater using Acrycoat L 100(r) solution as the coating agent. The pellets obtained were submitted to a dissolution test in HCl 0.1 N and phosphate buffer media. The results indicated that optimum gastric-resistance was only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rifampicin; Gastric-resistant isoniazids/pellets; Pellets/enteric coating; Acrycoat L 100(r)/use/enteric coating.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400749

Development and validation of alternative methods by non-aqueous acid-base titration and derivative ultraviolet spectrophotometry for quantification of sildenafil in raw material and tablets
Provedor de dados: 60
Autores: Silva,Taízia Dutra; Toledo,Cibele Rodrigues; Vianna-Soares,Cristina Duarte.
Sildenafil citrate (SILC) is a potent phosphodiesterase-5 inhibitor used for erectile dysfunction and pulmonary hypertension. This study shows two simple, fast and alternative analytical methods for SILC determination by non-aqueous titration and by derivative ultraviolet spectrophotometry (DUS) in active pharmaceutical ingredient and/or dosage forms. The quantitation method of SILC active pharmaceutical ingredient by non-aqueous acid-base titration was developed using methanol as solvent and 0.1 mol/L of perchloric acid in acetic acid as titrant. The endpoint was potentiometrically detected. The non-aqueous titration method shows satisfactory repeatability and intermediate precision (RSD 0.70-1.09%). The neutralization reaction occurred in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sildenafil citrate; Vasodilator; Derivative ultraviolet spectrophotometry; Non-aqueous acid-base titrimetry; Sildenafil citrate/quality control.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100608

13C NMR spectral data and molecular descriptors to predict the antioxidant activity of flavonoids
Provedor de dados: 60
Autores: Scotti,Luciana; Fernandes,Mariane Balerine; Muramatsu,Eric; Emereciano,Vicente de Paula; Tavares,Josean Fechine; Silva,Marcelo Sobral da; Scotti,Marcus Tullius.
Tissue damage due to oxidative stress is directly linked to development of many, if not all, human morbidity factors and chronic diseases. In this context, the search for dietary natural occurring molecules with antioxidant activity, such as flavonoids, has become essential. In this study, we investigated a set of 41 flavonoids (23 flavones and 18 flavonols) analyzing their structures and biological antioxidant activity. The experimental data were submitted to a QSAR (quantitative structure-activity relationships) study. NMR 13C data were used to perform a Kohonen self-organizing map study, analyzing the weight that each carbon has in the activity. Additionally, we performed MLR (multilinear regression) using GA (genetic algorithms) and molecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavonoids/antioxidant activity; 13C NMR; Kohonen self-organizing map.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200005

Economic model for obtaining cyclodextrins from commercial cgtase
Provedor de dados: 60
Autores: Koga,Luciana Numata; Fenelon,Vanderson Carvalho; Miyoshi,Juliana Harumi; Moriwaki,Cristiane; Wessel,Kamila Byanca Baldin; Mangolim,Camila Sampaio; Matioli,Graciette.
A repetitive batch process was employed followed by membrane ultrafiltration system to produce low-cost cyclodextrins (CDs) using commercial enzymes Toruzyme® cyclomaltodextrin glucanotransferase (CGTase) and its kinetic parameters were determined. The ultrafiltration system enabled the removalof inhibitory products from the reaction medium, allowing the enzyme to be recovered for reuse. A 10 kDa membrane was used to separate the different CDs produced by the CGTase. The substrates evaluated were maltodextrin, corn starch and cassava starch at 5, 10 and 15% (w/V), in the presence and absence of 10% (V/V) ethanol. After reaction for 132 h, 10% (w/V) cassava starch in the presence of ethanol provided the best results with 32.1 mg/mL of β-CD. Maximum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclodextrin; Commercial CGTase; Ultrafiltration; Repetitive batches; Starch.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100641

Dermocosmetic care for rosacea
Provedor de dados: 60
Autores: Gonçalves,Maria Manuel Baía de Melo Magalhães; Pina,Maria Eugénia Soares Rodrigues Tavares de.
ABSTRACT Rosacea is a chronic inflammatory skin disease that primarily affects the central area of the face; it is characterized by erythema, papules, pustules, nodules, and telangiectasia. This condition arises between 30-60 years of age, and it usually occurs in fair-skinned people. Rosacea is characteristic of sensitive skin, as it is a disease marked with punctuated phases of exacerbated signs and symptoms that alternate with periods of remission. Humans have long incorporated cosmetics in their daily habits; given the scientific and technological developments that emerged in cosmeceuticals or dermocosmetics, the current cosmetic options are now used for much more than adornment or cleansing. The purpose of cosmetic care in rosacea is to restore the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosacea/dermocosmetic treatment; Quality of life; Cosmetics; Cosmeceuticals; Skin care; Pharmacist advice.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400401

1,3,4-Thiadiazole and 1,2,4-triazole-3(4 H )-thione bearing salicylate moiety: synthesis and evaluation as anti- Candida albicans
Provedor de dados: 60
Autores: Radwan,Awwad Abdoh; Alanazi,Fares Kaed; Al-Agamy,Mohammed Hamed.
Abstract Dramatically increased occurrence of both superficial and invasive fungal infections has been observed. Candida albicans appear to be the main etiological agent of invasive fungal infections. The anti-C. albicans activity of thiosemicarbazide, 1,3,4-Thiadiazole, and 1,2,4-triazole-3(4H)-thione compounds (compounds 3-23) were investigated. The MIC values of thiadiazole and triazole derivatives 10-23 were in the range of 0.08-0.17 µmol mL-1, while that of fluconazole was 0.052 µmol mL-1. Compound 11 (5-(2-(4-chlorobenzyloxy)phenyl)-N-allyl-1,3,4-thiadiazol-2-amine) and compound 18 (5-(2-(4-chlorobenzyloxy)phenyl)-4-allyl-2H-1,2,4-triazole-3(4H)-thione) were found to be the most active compounds, with MIC values of 0.08 µmol mL-1. The newly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-Candida/docking studies; Thiadiazole; Triazole.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100614

Costus root aqueous extract modulates rat liver toxicity, DNA damage, injury, proliferation alterations induced by plant growth regulator Ethephon
Provedor de dados: 60
Autores: Tousson,Ehab; El-Atrsh,Afaf; Mansour,Merfaat; Assem,Abdallah.
Ethephon (Ethrel®) is an ethylene-based plant growth regulator that used in agriculture and it has direct and indirect effects on human health, direct effect via its inhalation during usage in agriculture and indirect effect through the diet (Fruits and vegetables) that is sprayed with it. The current study aimed to examine the possible modifying effects of costus (Saussurea lappa) root aqueous extract against Ethephon induced liver toxicity, injury, DNA fragmentation and PCNA alterations in male rats. Fifty adult male rats were divided into 5 groups (1st, control; 2nd, Costus; 3rd, Ethephon; 4th, Post treated Ethephon with costus; 5th, self-healing Ethephon). Current results revealed that; a significant increase in aspartate transaminase (AST), alanine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant growth regulator; Ethephon; Costus; Liver; DNA damage; PCNA expression; Rat.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100600

Determination of carbamazepine in pharmaceutical formulations
Provedor de dados: 60
Autores: Solon,Lílian Grace da Silva; Oliveira,Ana Isabel Maia de; Guerra,Gerlane Coelho Bernardo; Soares,Luiz Alberto Lira; Araújo,Aurigena Antunes de.
The aim of this study was to evaluate the quality of five different solid formulations of carbamazepine. The reference formulation was Tegretol® 200.00 mg (Novartis) and the others were: generic formulation of carbamazepine 200.00 mg (National Industry), similar formulation of carbamazepine 200.00 mg (National Industry), and two formulations of carbamazepine 200.00 mg acquired from two different compounding pharmacies. The latter consisted of capsules obtained in Natal, the capital city of the Brazilian State of Rio Grande do Norte. The quality of samples was evaluated through physical and physical-chemical tests, including: weight, diameter, thickness, content, dissolution, disintegration, hardness, friability and moisture. The results of friability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbamazepine; Medicines/quality control; Generic medicines; Similar medicines; Medicines/magistral formulation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300014

Exploratory study on sequestration of some essential metals by indigo carmine food dye
Provedor de dados: 60
Autores: Zanoni,Thalita Boldrin; Cardoso,Arnaldo Alves; Zanoni,Maria Valnice Boldrin; Ferreira,Antonio Aparecido Pupim.
Indigo carmine forms a stable complex with different ions, and the stability constant of the complexes were evaluated as log K equal to 5.75; 5.00; 4.89 and 3.89 for complexes with Cu(II), Ni(II), Co(II) and Zn(II) ions, respectively, in 0.1 mol L-1 carbonate buffer solution at pH 10. The interaction between Cu(II) ions and indigo carmine (IC) in alkaline medium resulted in the formation of the Cu2(IC) complex, measured by the spectrophotometric method, with a stoichiometric ratio between indigo carmine and metal ions of 2:1 (metal-ligand). The reported method has also been successfully tested for determination of copper in pharmaceutical compounds based on copper-gluconate without pre-treatment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metal sequestration; Indigo carmine; Food dye/complexation; Spectrophotometric analysis; Copper/determination.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400014

Efficacy of secnidazole-diminazene aceturate combination therapy in the late treatment of Trypanosoma brucei brucei infection in dogs
Provedor de dados: 60
Autores: Eke,Ifeanyi Gabriel; Ezeh,Ikenna Onyema; Ezeudu,Terry Adaeze; Eze,Ukamaka Uchenna; Anaga,Aruh Ottah; Onyeyili,Patrick Azubuike.
This study evaluated the efficacy of combination therapy of secnidazole-diminazene aceturate (SEC-DA) in late treatment of dogs experimentally infected with relapsing strain of Trypanosoma brucei brucei. Fifteen dogs were randomly assigned to 5 groups (A - E) of 3 per group. Group A (uninfected untreated), B (infected untreated), C (infected and treated with DA (3.5 mg/kg) IM stat), D (infected and treated with secnidazole (SEC) (100 mg/kg) orally for 5 days and DA (3.5 mg/kg) IM stat), E (infected and treated with SEC (200 mg/kg) orally for 5 days and DA (3.5 mg/kg) IM stat). Dogs were infected intraperitoneally with 5 x 105 trypanosomes and treatment started 14 days post-infection. Data on parasitaemia, hematology and rectal temperature were recorded....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Secnidazole; Diminazene aceturate; Combination therapy; Trypanosomosis; Dogs; Relapse.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100521

Drug-related problems observed in a pharmaceutical care service, Belo Horizonte, Brazil
Provedor de dados: 60
Autores: Nascimento,Yone de Almeida; Carvalho,Wânia da Silva; Acurcio,Francisco de Assis.
This present observational, longitudinal, and non-concurrent study was developed with the purposes of evaluate the profile of patients attended by a pharmacotherapeutic follow-up service and describe the Drug-Related Problems (DRPs) found over there; determine the proportion of DRPs between the health problems presented by the studied population, classifying them and identifying the situations related with their appearance. The study was developed at the School Pharmacy of Newton Paiva University Center, Belo Horizonte, MG, during the period from 2001 November up to 2003 November. Ninety seven patients have been evaluated, the majority of female sex (66.0%), with up to 8 years of scholarship (45.4%), mean age of 56.7 ± 13.0 years; mean of 4 ± 2 diagnosed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care; Pharmacotherapeutic follow-up; Drug Related Problem (DRP).
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200018

The in vivo effect of L-arginine on skin elasticity in mice
Provedor de dados: 60
Autores: Souza,Ádria do Prado Barros de; Oliveira,Marina Miranda Rocha de; Andrade,Rafael Rocha de; Amorim,Rivadávio Fernandes Batista de; Bocca,Anamélia Lonrezetti; Borin,Maria de Fátima.
ABSTRACT The human skin aging process is a complex mechanism that can be induced both by intrinsic and extrinsic factors. Observations include a decrease in the biosynthetic and proliferative capacity of cells, increased expression of matrix metalloproteinases, reduction in collagen type I expression, and the progressive disappearance of elastic tissue in the papillary dermis. L-arginine, the substrate of nitric oxide synthesis, is involved in angiogenesis and cell proliferation, as well as an indirect precursor of collagen synthesis via the proline pathway. The aim of this study was to examine the tensile strength, histology, and immunohistochemistry of female and male mice skin receiving different concentrations of topically applied L-arginine, in order...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-arginine; L-arginine/in vivo efficacy; Skin aging; Elastin; Collagen; INOS.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300615

Multidrug-resistant Candida glabrata strains obtained by induction of anidulafungin resistance in planktonic and biofilm cells
Provedor de dados: 60
Autores: Hatwig,Camila; Balbueno,Eduardo A.; Bergamo,Vanessa Z.; Pippi,Bruna; Fuentefria,Alexandre M.; Silveira,Gustavo P.
Candida glabrata has emerged as a common cause of serious life-threatening fungal infections, largely owing to their low susceptibility to azole antifungals. Recent guidance indicates the use of echinocandins as the first-choice drug for the treatment of systemic infections of C. glabrata; however, C. glabrata resistance to echinocandins is reportedly increasing. Herein, we present the induction of anidulafungin resistance in planktonic and sessile cells of C. glabrata and the development of fluconazole cross-resistance. MICs of 21 clinical C. glabrata strains were determined by a broth microdilution method using anidulafungin and fluconazole. Biofilm formation on a tracheal catheter was determined using 1- × 1-cm2 polyvinyl polychloride catheter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida glabrata; Biofilm; Anidulafungin; Cross-resistance; Fluconazole.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100604

Mucoadhesive drug delivery systems
Provedor de dados: 60
Autores: Carvalho,Flávia Chiva; Bruschi,Marcos Luciano; Evangelista,Raul Cesar; Gremião,Maria Palmira Daflon.
Drug actions can be improved by developing new drug delivery systems, such as the mucoadhesive system. These systems remain in close contact with the absorption tissue, the mucous membrane, releasing the drug at the action site leading to a bioavailability increase and both local and systemic effects. Mucoadhesion is currently explained by six theories: electronic, adsorption, wettability, diffusion, fracture and mechanical. Several in vitro and in vivo methodologies are proposed for studying its mechanisms. However, mucoadhesion is not yet well understood. The aim of this study was to review the mechanisms and theories involved in mucoadhesion, as well as to describe the most-used methodologies and polymers in mucoadhesive drug delivery systems.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucoadhesion; Bioadhesion; Mucoadhesive systems; Drugs/delivery.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100002

Cytotoxicity and antiviral activity evaluation of Cymbopogon spp hydroethanolic extracts
Provedor de dados: 60
Autores: Chiamenti,Lisandra; Silva,Francini Pereira da; Schallemberger,Karoline; Demoliner,Meriane; Rigotto,Caroline; Fleck,Juliane Deise.
Cymbopogon citratus and C. nardus are noteworthy among the several existing plant species displaying medicinal properties, due to the potential pharmacological activity of these species, including antiviral, antibacterial, antifungal and anti-trypanosomal activities. The objective of this study was to carry out in vitro toxicity tests of plant extracts from both species and analyze potential antiviral activity against Human mastadenovirus serotype 5 (HAdV-5). Two cell lines (A549 and VERO) were used and mitochondrial and lysosomal viability were determined by the MTT and neutral red assay, respectively, after two exposure times (24 hours and six days). The aim of these assays was to counteract the behavior of the extracts against the different cell lines...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cymbopogon citratus/toxicity; Cymbopogon nardus/toxicity; Cellular viability; Human mastadenovirus; Plants/extracts.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100539

Acetyl hexapeptide-3 in a cosmetic formulation acts on skin mechanical properties - clinical study
Provedor de dados: 60
Autores: Tadini,Kassandra Azevedo; Mercurio,Daiane Garcia; Campos,Patrícia Maria Berardo Gonçalves Maia.
abstract Acetyl hexapeptide-3 has been used in anti-aging topical formulations aimed at improving skin appearance. However, few basic studies address its effects on epidermis and dermis, when vehiculated in topical formulations. Thus, the objective of this study was to determine the clinical efficacy of acetyl hexapeptide-3 using biophysical techniques. For this purpose, formulations with and without acetyl hexapeptide-3 were applied to the ventral forearm and the face area of forty female volunteers. Skin conditions were evaluated after 2 and 4-week long daily applications, by analyzing the stratum corneum water content and the skin mechanical properties, using three instruments, the Corneometer(r) CM 825, CutometerSEM 575 and ReviscometerRV600. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetyl hexapeptide-3/use in cosmetic formulations; Acetyl hexapeptide-3/clinical efficacy; Biophysical techniques; Anti-aging/cosmetics; Clinical efficacy; Cosmetics.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400901

Formulation design and evaluation of Cefuroxime axetil 125 mg immediate release tablets using different concentration of sodium lauryl sulphate as solubility enhancer
Provedor de dados: 60
Autores: Israr,Fozia; Mahmood,Zafar Alam; Hassan,Fouzia; Hasan,Syed Muhammad Farid; Jabeen,Sabahat; Naz,Shazia; Bashir,Lubna.
Cefuroxime axetil immediate release tablets were formulated by direct compression method with different percentages of sodium lauryl sulphate (SLS) such as 0.5, 1.0, 1.5 and also without SLS. Resulting batches of tablets were evaluated by both pharmacopeial and non-pharmacopeial methods to ascertain the physico-mechanical properties. Dissolution test were carried out in different medium like 0.07 M HCl, distilled water, 0.1M HCl of pH 1.2 and phosphate buffers at pH 4.5 and 6.8 to observe the drug release against the respective concentration of SLS used. Later, test formulations were compared by f1(dissimilarity) and f2(similarity) factors using a reference brand of cefuroxime axetil. Significant differences (p&lt;0.05) in dissolution rate were recorded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cefuroxime axetil/immediate release tablets/physico-mechanical properties; Direct compression; Sodium lauryl sulphate.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400943

Solubility and dissolution studies of tibolone polymorphs
Provedor de dados: 60
Autores: Bonfilio,Rudy; Souza,Marília Cristina Oliveira; Leal,Jockastta Silva; Viana,Olímpia Maria Martins Santos; Doriguetto,Antônio Carlos; Araújo,Magali Benjamin de.
ABSTRACT Different solid forms of an active pharmaceutical ingredient can have distinct chemical and physical characteristics. In this work, we studied the solubility and dissolution properties of the described tibolone polymorphic forms (I and II). Both forms were successively recrystallized and characterized by powder X-ray diffraction and attenuated total reflection infrared spectroscopy. Equilibrium solubility and dissolution profiles were performed for both forms. Solubility studies demonstrated that form II is statistically more soluble in water, 0.01 mol L-1 HCl and pH 4.5 acetate buffer. The solubility of forms I and II were explained in terms of crystal packing. Dissolution tests of tablets showed a lower release of polymorphic form II than form I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tibolone/polymorphs; Powder X-ray diffraction/methods; Infrared spectroscopy/methods; Solubility/drug effects; Dissolution/analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400607

Effects of administering testosterone undecanoate in rats subjected to physical exercise: effects on the estrous cycle, motor behavior and morphology of the liver and kidney
Provedor de dados: 60
Autores: Bento-Silva,Moisés Tolentino; Martins,Maria do Carmo de Carvalho e; Torres-Leal,Francisco Leonardo; Barros,Talvany Luiz; Carvalho,Ingrid Lara do Nascimento Ferreira de; Carvalho Filho,Hugo Aparecido; Almeida,Fernanda Regina de Castro.
The aim of the work was evaluate the effects of testosterone undecanoate (TU) treatment combined with moderate physical training on: the estrous cycle, body weight (BW), motor behavior (MB), and the morphohistology of the reproductive system, the liver and kidney in rats. Female Wistar rats (180 g - 250 g) were divided as follows: sedentary + TU (S + TU), trained + TU (T + TU), sedentary + vehicle (S + V), trained + vehicle (T + V). The rats swam 50 min/Day, strapped with a 5% BW load, for 4 weeks. During this training, (BW) was monitored daily as well as the estrous cycle (EC) by vaginal smear. The TU (15 mg/kg s.c) was administered 3 times/week for 4 weeks. At the end of the study, data on MB, BW and morphohistopathological changes in viscera were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Androgenic anabolic steroids/experimental study; Endurance training; Testosterone undecanoate.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100009

Augmented cytotoxic, mutagenic and genotoxic response triggered by carvedilol and celecoxib combinations
Provedor de dados: 60
Autores: Attiq,Ali; Ashraf,Muhammad; Jalil,Juriyati; Javeed,Aqeel; Anjum,Aftab Ahmad; Ullah,Asad; Umair,Muhammad; Ali,Sarwat.
Abstract It is understood that drugs regardless of their order of administration can exhibit drug interactions. Established on the fact that treatment of hypertension may last for decades and prolong usage of multiple drug regimen may induce substantial pathophysiological changes. Hence, This study was designed to evaluate the possible synergistic toxic effects of anti-hypertensive (carvedilol), and anti-inflammatory drug (celecoxib) alone and in combinations. Well-established MTT assay, Single Cell Gel Electrophoresis (SCGE) and Ames assay were employed to evaluate the toxicity at cellular level. Results from MTT assay on Vero cell line revealed that drug combinations have more pronounced anti-proliferative activity with combine IC50 value of 13.7:47.8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carvedilol; Celecoxib; Mutagenicity; Genotoxicity; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100622

Improved dissolution and micromeritic properties of naproxen from spherical agglomerates: preparation, in vitro and in vivo characterization
Provedor de dados: 60
Autores: Saritha,Damineni; Bose,Penjuri Subhash Chandra; Reddy,Poreddy Srikanth; Madhuri,Grandhi; Nagaraju,Ravouru.
Naproxen, an anti-inflammatory drug, exhibits poor aqueous solubility, which limits the pharmacological effects. The present work was carried out to study the effect of agglomeration on micromeritic properties and dissolution. Naproxen agglomerates were prepared by using a three solvents system composed of acetone (good solvent), water (non-solvent) and dichloromethane (bridging liquid). Differential Scanning Calorimetry (DSC) results showed no change in the drug after crystallization process. X-Ray Powder Diffraction (XRPD) studies showed the sharp peaks are present in the diffractograms of spherical agglomerates with minor reduction in height of the peaks. The residual solvents are largely below the tolerated limits in the agglomerates. Scanning...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naproxen/micromeritic properties; Naproxen/spherical agglomerates; Naproxen/dissolution; Residual solvents; Ulcerogenic potential; Bridging liquid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400010

Synthesis, spectral analysis and pharmacological study of N'- substituted-2-(5-((2,4-dimethylphenoxy)methyl)-1,3,4-oxadiazol-2-ylthio)acetohydrazides
Provedor de dados: 60
Autores: Rasool,Shahid; Aziz-ur-Rehman,; Abbasi,Muhammad Athar; Siddiqui,Sabahat Z; Shah,Syed Adnan Ali.
ABSTRACT A series of molecules bearing multiple functional groups were synthesized to study their antibiotic effect against Gram-positive and Gram-negative bacteria and lipoxygenase activity as well. 2,4-Dimethylcarbolic acid (1) was refluxed with ethyl 2-bromoacetate to synthesize ethyl 2-(2,4-dimethylphenoxy)acetate (2). Compound 2 was converted to the corresponding hydrazide 3, again on refluxing with hydrazine. The compound 5-((2,4-dimethylphenoxy)methyl)-1,3,4-oxadiazol-2-thiol (4) was synthesized by the reaction of 3 and CS2 in the presence of KOH. Compound 4 was further converted to the corresponding ester 5 and then 2-(5-((2,4-dimethylphenoxy)methyl)-1,3,4-oxadiazol-2-ylthio)acetohydrazide (6). The final molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetohydrazides/ Synthesis; Acetohydrazides/Antibacterial activity/ Acetohydrazides/Lipoxygenase/inhibition activity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300471

Antineoplastic potential of the aqueous crude extract of Eugenia uniflora L. in human cervical cancer
Provedor de dados: 60
Autores: Núñez,Jisette González; Pinheiro,Jordânia dos Santos; Silveira,Gabriel Fernandes; Beckenkamp,Aline; Buffon,Andréia; Bruno,Alessandra Nejar.
Abstract Considering the high prevalence of human cervical cancer and the adverse effects of the available treatments, it is important to develop studies involving plants. Eugenia uniflora L. is a Brazilian native plant widely used in folk medicine and some biological effects have already been described. In this study, we investigated the biologicals effects of the aqueous crude extract of E. uniflora leaves in relation to the viability of human cervical cancer cells (SiHa), non-tumorigenic cells HaCaT and human lymphocytes. Our results demonstrated that different concentrations of E. uniflora’s extract significantly inhibited the viability of the Siha cell line at 24, 48 and 72 hours of treatment, but did not induce significant changes in the HaCat cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenia uniflora L./effects/antitumoral; Pitangueira; SiHa; HaCat; Plant extract/antitumoral; Cervical cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200618

Safety assessment and behavioral effects of Solanum guaraniticum leaf extract in rats
Provedor de dados: 60
Autores: Bonfanti,Gabriela; Bitencourt,Paula Eliete Rodrigues; Bona,Karine Santos De; Peroza,Luis Ricardo; Cargnelutti,Lariane Oliveira; Borges,Raphaela Maleski; Pigatto,Aline Grohe Schirmer; Fachinetto,Roselei; Boligon,Aline Augusti; Athayde,Margareth Linde; Gonçalves,Thissiane de Lima; Moretto,Maria Beatriz.
ABSTRACT Solanum guaraniticum is a medicinal plant traditionally used to treat gastric and liver diseases. However, there is no documented evidence corroborating its safety. The present study evaluated the potential toxicity of S. guaraniticum leaf extract after acute administration in rats. Single doses of the extract (1.250, 2.500, and 5.000 mg/kg) were administered by gavage, and the rats were then monitored for 48 h and/or 14 days. Mortality, acute signs of toxicity, and general activity in the open field test were assessed as well as hematological and biochemical parameters, enzymatic activity (δ-aminolevulinate dehydratase and acetylcholinesterase), and oxidative stress parameters (lipid peroxidation level, non-protein thiol content, tissue catalase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum guaraniticum/leaf extract/toxicity; Solanum guaraniticum/phytochemistry; Medicinal plants; Solanaceae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100006

Evaluation of pharmaceutical assistance in municipalities in the state of Minas Gerais
Provedor de dados: 60
Autores: Moura,Cristiano Soares de; Perini,Edson.
The study proposal was to evaluate the pharmaceutical assistance in the state of Minas Gerais (MG) utilizing pharmaceutical assistance indicators described in the literature. The evaluated items were: organization, financing, selection, acquisition, programming, distribution, availability and storage of medicines in 18 municipalities of the Regional Management of Health of Belo Horizonte. The results have shown good performance respecting service organization, presence and updating of Municipal Essential Drug List. The main problems were related to stock control and availability of some items. The work has demonstrated the operational viability of indicators, as to evaluate the conditions of pharmaceutical assistance, as to monitor their performance.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical assistance; Health services/evaluation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200013

Drug related problems associated with the psychoactive drugs used on geriatric, hospitalized patients
Provedor de dados: 60
Autores: Irma Leonor,Ortega López; Miguel Ernesto,Verdecia Rosés.
A prospective study was carried out, to identify the drug related problems and to prevent or to solve the negative outcomes associated with medication of the geriatric patients that consumed psychoactive drugs, who were hospitalized. The study was carried out according to the DADER Method of Pharmacotherapy Monitoring, adapted to the Hospital's environment and the theoretical rationale of the Third Consent of Granada was assumed, for the considerations related to the drug related problems and the negative outcomes associated with medication. Thirty five patients were included whose ages ranged between the 60 and 90 years. Thirty six negative associated outcomes were identified, in 34 patients that represented 97.14% incidence. A prevalence of the negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medication/associated negative outcomes; Psychoactive drugs/geriatric use; Geriatric patients/use of psychoactive drugs.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300012

Determination of the worst case for cleaning validation of equipment used in the radiopharmaceutical production of lyophilized reagents for 99mTc labeling
Provedor de dados: 60
Autores: Porto,Luciana Valéria Ferrari Machado; Fukumori,Neuza Taeko Okasaki; Matsuda,Margareth Mie Nakamura.
ABSTRACT Cleaning validation, a requirement of the current Good Manufacturing Practices (cGMP) for Drugs, consists of documented evidence that cleaning procedures are capable of removing residues to predetermined acceptance levels. This report describes a strategy for the selection of the worst case product for the production of lyophilized reagents (LRs) for labeling with 99mTc from the Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/São Paulo). The strategy is based on the calculation of a "worst case index" that incorporates information about drug solubility, cleaning difficulty, and occupancy rate in the production line. It allowed a reduction in the required number of validations considering the possible manufacturing flow of a given product...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals/production; Drugs/manufacturing/cleaning validation; Lyophilized reagents.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100012

Development and optimization of self microemulsifying drug delivery of domperidone
Provedor de dados: 60
Autores: Laddha,Pankaj; Suthar,Vrunda; Butani,Shital.
The present investigation is aimed to develop self-microemulsifying drug delivery system (SMEDDS) to improve the in vitro dissolution of a BCS (Biopharmaceutical Classification System) class II anti emetic agent, domperidone. Solubility study was performed to identify the ingredients showing highest solubility of domperidone. The ternary phase diagrams were plotted for selected components to identify the area of microemulsion existence. D-optimal mixture experimental design was applied to optimize a liquid SMEDDS using formulation variables; the oil phase X1 (Oleic acid), the surfactant X2 (Labrasol) and the co-surfactant X3 (Transcutol HP). The liquid SMEDDS were evaluated for droplet size, emulsification time, % transmittance and drug release. Stability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domperidone/self-microemulsifying delivery; Self-microemulsifying drug delivery system/development; Biopharmaceutical Classification System.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100009

Clinacanthus nutans: Its potential against diabetic vascular diseases
Provedor de dados: 60
Autores: Azemi,Ahmad Khusairi; Mokhtar,Siti Safiah; Rasool,Aida Hanum Ghulam.
Diabetes is an independent risk factor for the development of cardiovascular disease, with approximately 80% of cardiovascular mortality and morbidity linked to vascular complications such as atherosclerosis. It has been estimated that up to one-third of patients with diabetes mellitus use some form of complementary and/or alternative medicine. One plant that has received attention from diabetic patients for its perceived antidiabetic properties is Clinacanthus nutans, a member of the Acanthaceae family that is known as snake grass. Ethnomedical applications of this herb have been identified for the treatment of certain conditions, including fever, diabetes, skin rashes, and insect bites. This review aims to assess the potential of C. nutans to be used in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. nutans; Diabetes; Inflammation; Cardiovascular disease.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100635

Quantification of glucosylceramide in plasma of Gaucher disease patients
Provedor de dados: 60
Autores: Muller,Maria Viviane Gomes; Petry,André; Vianna,Luciene Pinheiro; Breier,Ana Carolina; Michelin-Tirelli,Kristiane; Pires,Ricardo Flores; Trindade,Vera Maria Treis; Coelho,Janice Carneiro.
Gaucher disease is a sphingolipidosis that leads to an accumulation of glucosylceramide. The objective of this study was to develop a methodology, based on the extraction, purification and quantification of glucosylceramide from blood plasma, for use in clinical research laboratories. Comparison of the glucosylceramide content in plasma from Gaucher disease patients, submitted to enzyme replacement therapy or otherwise, against that from normal individuals was also carried out. The glucosylceramide, separated from other glycosphingolipids by high performance thin layer chromatography (HPTLC) was chemically developed (CuSO4 / H3PO4) and the respective band confirmed by immunostaining (human anti-glucosylceramide antibody / peroxidase-conjugated secondary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucosylceramide; Blood plasma analysis; Gaucher disease.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400005

Floating microspheres: a review
Provedor de dados: 60
Autores: Mukund,Jagtap Yogesh; Kantilal,Bhujbal Rohan; Sudhakar,Ranpise Nisharani.
Gastric emptying is a complex process, one that is highly variable and that makes in vivo performance of drug delivery systems uncertain. A controlled drug delivery system with prolonged residence time in the stomach can be of great practical importance for drugs with an absorption window in the upper small intestine. The main limitations are attributed to the inter- and intra-subject variability of gastro-intestinal (GI) transit time and to the non-uniformity of drug absorption throughout the alimentary canal. Floating or hydrodynamically controlled drug delivery systems are useful in such applications. Various gastroretentive dosage forms are available, including tablets, capsules, pills, laminated films, floating microspheres, granules and powders....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Floating microspheres; Emulsion solvent diffusion-evaporation method; Drug delivery system; Release modifiers.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100003

Use of a commercial agarose gel for analysis of urinary glycosaminoglycans in mucopolysaccharidoses
Provedor de dados: 60
Autores: Breier,Ana Carolina; Cé,Jaqueline; Coelho,Janice Carneiro.
ABSTRACT Mucopolysaccharidoses (MPS) are a group of inherited metabolic disorders caused by deficiency of enzymes that degrade glycosaminoglycans (GAGs). Urinary excretion of GAGs is a common feature of MPS, and is considered their major biomarker. We aimed to adapt the GAG electrophoresis method to a commercial agarose gel which would be able to separate urinary GAGs in a simpler way with good sensitivity and reproducibility. Urine samples from patients previously diagnosed with MPS I, IV, and VI were used as electrophoretic standards. Samples from patients on enzyme replacement therapy (ERT) were also assessed. Commercial agarose gel electrophoresis was effective, showing proper definition and separation of GAG bands. Detection sensitivity exceeded 0.1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopolysaccharidoses/tests/treatment; Electrophoresis/monitoring method; Glycosaminoglycans/use.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400693

Effect of wet mass on dextromethorphan hydrobromide matrix pellets
Provedor de dados: 60
Autores: Ibrahim,Mohamed Abbas; Mahrous,Gamal Mohamed.
Dextromethorphan hydrobromide (DM) sustained release matrix pellets containing 10% w/w drug were prepared by an extrusion/spheronization technique. The effect of mixing different concentrations of ethyl cellulose (EC), hydroxypropyl methylcellulpse (HPMC K10), and Carbopol 934 with Avicel PH101 on the rheological properties of pellet wet mass was evaluated using mixer torque rheometry (MTR). The prepared pellets were characterized for size, drug content, and in-vitro DM release rate. The results showed that increasing the concentration of the hydrophobic polymer (EC) with Avicel PH101 decreased wet mass consistency, represented by mass mean line torque. Lower binder ratio was required for optimum wet massing, while mixing with swellable polymers (HPMC and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dextromethorphan hydrobromide; Matrix pellets; Extrusion spheronization; Mix torque rheometer (MTR).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400604

Development and characterization of photoprotective formulations containing keratin particles
Provedor de dados: 60
Autores: Teixeira,Jessica; Freitas,Zaida Maria Farias de; Monteiro,Mariana Sato de Souza Bustamante; Vermelho,Alane Beatriz; Ricci Junior,Eduardo; Santos,Elisabete Pereira dos.
This work deals with development and evaluation of MFQ protective formulation, which contains two organic filters, namely: octyl-p-methoxycinnamate (OMC) and benzophenone-3 (BP-3); a photostabilizing agent called ethylhexylmethoxycrylene (EHMCR) and keratin particles. The MFQ formulation was evaluated in order to measure its pH, spin-spin lattice relaxation time (T2H), occlusivity factor, formulation efficacy, photostability and skin permeation, as well as keratin particle properties. Keratin particle size increased when incorporated to formulation, however, it did not affect pH. The MFQ formulation was found to be photostable and photoprotective, as evidenced by sunlight photostability test, sun protection factor (SPF), UVA/UVB ratio and critical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sunscreen; Keratin; Octylmethoxycinnamate; Benzophenone-3; Ethylhexylmethoxycrylene.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100597

Development of sunscreen products containing passion fruit seed extract
Provedor de dados: 60
Autores: Lourith,Nattaya; Kanlayavattanakul,Mayuree; Chingunpitak,Jiraporn.
Abstract Application of sunscreen is the most established method of protecting skin from premature aging and photoaging. In this study, the passion fruit seed extract, enriched with biologically beneficial phenolics, was formulated into sun-protective makeup product. The UVB protection of concealer mousse was found have twofold higher sun protection factor (SPF) than the liquid foundation (15.48 ± 1.60 and 5.88 ± 0.30, respectively). The SPF of concealer mousse as well as the liquid foundation containing 0.1% and 0.3% of the passion fruit seed extract were 18.75 ± 0.28, 18.99 ± 0.71 and 9.32 ± 0.88, 9.77 ± 1.37, respectively. Therefore, the concealers with a similar sun-protective efficacy (p&gt;0.05) were included for stability test accordingly. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmetics/study; Photoprotection/sun protection; Photoprotection/products development; Passion fruit/applications.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100625

Effect of cellulose acetate phthalate and polyethylene glycol on physical properties and release of theophylline from microcapsules
Provedor de dados: 60
Autores: Hussain,Amjad; Mumtaz,Ahmad Mehmood; Arshad,Muhammad Sohail; Abbas,Nasir; Latif,Abida; Shamim,Rahat; Bukhari,Nadeem Irfan; Hussain,Khalid.
ABSTRACT The present study describes the development of theophylline microcapsules by a non-solvent addition method and the effect of plasticizer addition on microencapsulation. The release was studied in distilled water and the data were analysed by various mathematical models for determining the mechanism of release. Prepared microcapsules were found to be spherical, free flowing and having more than 80% entrapped drug. The polymer - cellulose acetate phthalate and plasticizer - polyethylene glycol was considered to be affecting the properties of microcapsules including drug release (time for 50% drug release, T50). The formulation with the highest proportion of polymer and without plasticizer (F3) showed the slowest release with T50 = 4.3 h, while the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Theophylline/microcapsules; Microencapsulation; Cellulose acetate phthalate; Polyethylene glycol; Microcapsules/mechanism of release; Drugs/release.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100004

Simultaneous determination of ethinyl estradiol and drospirenone in oral contraceptive by high performance liquid chromatography
Provedor de dados: 60
Autores: Silva,Viviane Benevenuti; Galdos,Angel Arturo Gaona; Mothe,Cintia Maria Alves; Pallastrelli,Michele Bacchi; Prado,Maria Segunda Aurora; Singh,Anil Kumar; Kedor-Hackmann,Erika Rosa Maria; Santoro,Maria Inês Rocha Miritello.
A simple, rapid, economical and reliable high performance liquid chromatographic method has been developed and successfully applied in simultaneous determination of ethinyl estradiol and drospirenone in coated tablets. The HPLC method was performed on a LiChroCART® 100RP column (125x4 mm i.d., 5 µm) with acetonitrile:water 50:50 (v/v) as mobile phase, pumped at a flow rate of 1.0 mL.min-1. The fluorescence detection for ethinyl estradiol was made at λex= 280 nm and λem= 310 nm and a UV detection for drospirenone was made at 200 nm. The elution time for ethinyl estradiol and drospirenone were 4.0 and 5.7 min, respectively. The method was validated in accordance to USP 34 guidelines. The proposed HPLC method presented advantages over reported methods and is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coated tablets/quantitative analysis; High performance liquid chromatography/quantitative analysis; Ethinyl estradiol/quantitative determination; Drospirenone/quantitative determination; Fluorescence detection; UV detection.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300013

Evaluation of the Brazilian Cosmetic Legislation and its impact on the industries during the 20th century
Provedor de dados: 60
Autores: Garbossa,Wanessa Almeida Ciancaglio; Campos,Patrícia Maria Berardo Gonçalves Maia.
ABSTRACT Cosmetics have been used since the very first days of human life. This early and continuing consumption has resulted in the growth of the Cosmetic Industry (C.I.). The C.I. must continue to invest in the development of innovative products which are safe for the consumers. On the other hand, the Health Agency should issue laws regulating the development of products. Thus, the aim of this study was to evaluate the Brazilian Cosmetic Legislation before ANVISA and the challenges for the C.I. during the 20th century regarding the development of safe and effective cosmetics, and to show the importance of this legislation in the R&amp;D. A Bibliographic and Documentary Exploratory Research was conducted based on safety and efficacy data contained in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil/Sanitary Agency/cosmetics production; Brazil/cosmetic legislation; Cosmetics/safety production; Cosmetic industry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200319

To be or not to be a carcinogen; delving into the glyphosate classification controversy
Provedor de dados: 60
Autores: Paumgartten,Francisco José Roma.
The International Agency for Research on Cancer (IARC) placed the most widely used herbicide glyphosate (GLY) into the category 2A (probably carcinogenic to humans), a classification questioned by experts from academia and industry. This article critically appraised the epidemiological and experimental data that led the IARC working group (WG) to consider GLY a probable human carcinogen and the ensuing controversy. An association of GLY with non-Hodgkin lymphoma was suggested by some observational studies. A non-causal explanation for this weak association, however, cannot be excluded. Contrary to WG’s view, long-term rodent assays yielded no convincing evidence that GLY is carcinogenic. The mechanistic evidence remains elusive as well. Bacterial reverse...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticides/ toxicity; Glyphosate/ adverse effects; Carcinogens; Cancer risk; Hazard/adverse effects; Genotoxicity/prevention and control.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100510

Synthesis of novel papulacandin D analogs and evaluation of their antifungal potential
Provedor de dados: 60
Autores: Bretas,Ana Carolina Oliveira; Souza,Thiago Belarmino de; Borelli,Beatriz; Johan,Suzana; Alves,Ricardo José.
Systemic fungal infections are a growing problem in contemporary medicine and few drugs are licensed for therapy of invasive fungal infections. Differences between fungi and humans, like the presence of a cell wall in fungal cells, can be explored for designing new drugs. (1,3)-β-D-glucan synthase, an enzyme that catalyzes the synthesis of (1,3)-β-D-glucan, a structural and essential component of the fungal cell wall, is absent in mammals and this makes it an excellent target for the development of new antifungal agents. Papulacandins are a family of natural antifungal agents targeting (1,3)-β-D-glucan synthase. In this study we describe the synthesis and biological evaluation of two new Papulacandin analogs as potential (1,3)-β-D-glucan synthase inhibitors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-(1; 3)-D-glucan synthase Antifungal activity Papulacandin D Molecular simplification.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100509

Contribuição do uso de medicamentos para a admissão hospitalar
Provedor de dados: 60
Autores: Mastroianni,Patrícia de Carvalho; Varallo,Fabiana Rossi; Barg,Marina Souza; Noto,Ana Regina; Galduróz,José Carlos Fernandez.
As Reações Adversas a Medicamentos (RAM) podem ser responsáveis por 2,4% a 11,5% das admissões hospitalares. O estudo objetivou conhecer o perfil demográfico dos pacientes internados por possíveis RAM, identificar os medicamentos e as queixas mais freqüentemente relacionadas e estimar a incidência de admissão hospitalar pelo uso de medicamentos. Durante um mês, os pacientes internados em um hospital geral foram entrevistados quanto ao uso de medicamentos antes da internação e as queixas que o levaram ao hospital; as informações foram analisadas, usando-se a base de dados MICROMEDEX® e outras bases oficiais. Observou-se que as admissões por uso de medicamentos ocorreram predominantemente em idosos [47,5% (66/139)] e mulheres [62% (87/139)]. Os medicamentos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicamentos/uso racional; Medicamentos/reações adversas; Atenção farmacêutica; Acompanhamento farmacoterapêutico.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100020

An alternate technique for isolation of Toxocara canis excretory-secretory antigens
Provedor de dados: 60
Autores: Colli,Cristiane Maria; Elefant,Guita Rubinsky; Paludo,Marcia Liz; Guilherme,Ana Lúcia Falavigna.
The aim of the present study was to test the effectiveness of a sausage-casing membrane for dialysis of Toxocara excretory-secretory antigens (TES). The protein concentrated by the tested membrane was compared with that obtained using a Sigma commercial membrane, as were the protein fractions found by polyacrylamide gel electrophoresis. Standard positive and negative serum samples were evaluated in an ELISA immunoassay, and equivalent data were obtained in all steps, indicating that the sausage-casing membrane is efficient, besides being less expensive to process.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxocara canis/excretory-secretory antigens; Antigens/isolation; Dialysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100015

Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity
Provedor de dados: 60
Autores: Meneguello,Jean Eduardo; Silva,João Vitor de Oliveira; Lima,Letícia Silva; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin de Lima; Caleffi-Ferracioli,Katiany Rizzieri; Cardoso,Rosilene Fressatti.
ABSTRACT Microscopy and bacterial culture are the main tools in the diagnosis of tuberculosis. Since the slow growth of Mycobacterium tuberculosis impairs rapid diagnosis strategies, especially in countries where the latter are the only available resources, the ongoing development of new and inexpensive tools based on mycobacterial metabolism optimizing growth detection with preliminary identification is greatly welcome. When compared to the other species from the M. tuberculosis complex, M. tuberculosis is a strong nitrate reducer. Current assay compares the nitrate reductase activity of M. tuberculosis from pulmonary specimens cultivated in nitrate-supplemented media. Fifty-five sputum samples were decontaminated and inoculated in conventional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mycobacteria/diagnosis/identification; Nitrate reductase.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100602

Molecular biology of human epidermal receptors, signaling pathways and targeted therapy against cancers: new evidences and old challenges
Provedor de dados: 60
Autores: Ferreira,Paulo Michel Pinheiro; Pessoa,Cláudia.
ABSTRACT Human epidermal receptors (HER1/2/3/4) belong to the class of receptor-type tyrosine kinases. After binding a ligand, dimerization, it will ocurr activation of intracellular kinases after two-dimensional and cytoplasmic tail reciprocal transphosphorylation. This transphosphorylation recruits signaling pathways such as Ras/Raf/MEK/Erk1-2, PI3-K/AKT and JAK/STAT, which can affect the cell cycle, cytoskeleton reorganization, apoptosis, metastasis, differentiation, angiogenesis and transcription. HER deregulation is found in epithelial, mesenchymal and nervous neoplasms and is associated with poor prognosis and tumor severity. Since HER are promiscuous proteins when subjected to mutations, resultant modifications confer cellular metabolic superiority...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human epidermal receptors/molecular biology; Tyrosine kinase; Cancers/resistance; Chemotherapy; Therapeutic strategies.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200630

Development of radioimmunoconjugate for diagnosis and management of head-and-neck subclinical cancer and colorectal carcinoma
Provedor de dados: 60
Autores: Benedetto,Raquel; Massicano,Adriana Vidal Fernandes; Silva,Jefferson Jesus; Boas,Cristian Antonio Wieczorek Villas; Mengatti,Jair; Araújo,Elaine Bortoleti de.
ABSTRACT Scientific innovations in diagnostic methods are important drivers of cancer control and prevention. Noninvasive imaging of the epidermal growth factor receptor (EGFR) in head-and-neck squamous, cell carcinoma and colorectal cancer could be valuable to select patients for EGFR-targeted therapy, as well as to monitor the efficacy and occurrence of resistance to immunotherapy. In order to develop the first Brazilian radioimmunoconjugate for diagnosis, Cetuximab has been conjugated to p-SCN-Bn-DTPA chelator and radiolabeled with Indium-111. The conjugation methodology was optimized using different mAb:DTPA molar ratios, time was then reduced for immunoconjugate preparation, besides the protein recovery’ percentage increased after purification (m =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioimmunoconjugate/development; Head and neck cancer/diagnosis; Tumors diagnosis; Cetuximab; SPECT; 111In.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400624

Assessment of anti-diabetic activity of a novel hydrazine-thiazole derivative: in vitro and in vivo method
Provedor de dados: 60
Autores: Resende,Marina Ferrara de; Lino,Cleudiomar Inácio; Souza-Fagundes,Elaine Maria de; Rettore,João Vitor Paes; Oliveira,Renata Barbosa de; Labanca,Renata Adriana.
Diabetes mellitus is a chronic disease resulting in oxidative stress that promotes tissue damage. The appearance of this disease is highly related to lifestyle and food of the population, being of great interest to search for a dietary supplement that can also act by reducing oxidative alterations. Based on the broad range of biological activity of thiazole derivatives, this work aimed to evaluate the in vitro antioxidant activity of a novel hydrazine-thiazole derivative and studies in vivo. In in vivo experiments, the liver extracts of healthy and diabetic Wistar rats were used, with analysis to determine the enzymatic activity of SOD, CAT, GPx, and GR, and determination of lipid peroxidation. Finally, in the blood of these animals, biochemical parameters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Diabetes mellitus; Curcumin; Thiazole derivatives; Dietary supplement.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100509

Prednisone raw material characterization and formulation development
Provedor de dados: 60
Autores: Toehwé,Leonardo Henrique; Prado,Livia Deris; Rocha,Helvécio Vinícius Antunes.
ABSTRACT Solid dosage forms for oral use, particularly tablets, are the most highly used dosage forms in therapy because they are easily administered, have high productivity and relatively low cost and provide a more stable drug to form a semi-solid net. Numerous parameters influence the quality of the final dosage form. In this study, the dissolution profile of 20-mg prednisone tablets bioequivalent to the reference product and three test formulations were evaluated using stability testing. During the study, prednisone tablets and the active pharmaceutical ingredient (API) prednisone from two different manufacturers were characterized with respect to their physical and physicochemical properties. The results showed that the dissolution profiles of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prednisone/tablets/dissolution; Solubility; Formulation; Stability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400604

Biological activity of synthesized 5-{1-[(4-chlorophenyl)sulfonyl]piperidin-4-yl}-2-mercapto-1,3,4-oxadiazole derivatives demonstrated by in silico and BSA binding studies
Provedor de dados: 60
Autores: Iqbal,Javed; Rehman,Aziz-ur-; Abbasi,Muhammad Athar; Siddiqui,Sabahat Zahra; Rasool,Shahid; Ashraf,Muhammad; Iqbal,Ambar; Hamid,Sujhla; Chohan,Tahir Ali; Khalid,Hira; Laulloo,Sabina Jhaumeer; Shah,Syed Adnan Ali.
We synthesized a series of compounds bearing pharmacologically important 1,3,4-oxadiazole and piperidine moieties. Spectral data analysis by 1H-NMR, 13C-NMR, IR and EI-MS was used to elucidate the structures of the synthesized molecules. Docking studies explained the different types of interaction of the compounds with amino acids, while bovine serum albumin (BSA) binding interactions showed their pharmacological effectiveness. Antibacterial screening of these compounds demonstrated moderate to strong activity against Salmonella typhi and Bacillus subtilis but only weak to moderate activity against the other three bacterial strains tested. Seven compounds were the most active members as acetyl cholinesterase inhibitors. All the compounds presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 1; 3; 4-Oxadiazole Acetylcholinesterase (AChE) inhibition Antibacterial activity Piperidine Sulfonamide Urease inhibition.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100596

In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species
Provedor de dados: 60
Autores: Martins,Monica Lacerda Lopes; Pacheco,Henrique Poltronieri; Perini,Iara Giuberti; Lenz,Dominik; Andrade,Tadeu Uggere de; Endringer,Denise Coutinho.
In 1820, French naturalist August Saint Hillaire, during a visit in Espírito Santo (ES), a state in southeastern Brazil, reported a popular use of Cyperaceae species as antidote to snake bites. The plant may even have a hypotensive effect, though it was never properly researched. The in vitro inhibitory of the angiotensin converting enzyme (ACE) activity of eigth ethanolic extracts of Cyperaceae was evaluated by colorimetric assay. Total phenolic and flavonoids were determined using colorimetric assay. The hypotensive effect of the active specie (Rhychonospora exaltata, ERE) and the in vivo ACE assay was measured in vivo using male Wistar Kyoto (ERE, 0.01-100mg/kg), with acetylcholine (ACh) as positive control (5 µg/kg, i.v.). The evaluation of ACE in vivo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyperaceae/species; Cyperaceae/pharmacognosy; Cyperaceae/species; Cyperaceae/in vitro inhibitory activity; Cyperaceae/species; Cyperaceae/in vivo hypotensive effect; Angiotensin converting enzyme/in vitro inhibitory; Flavonoids/determination.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400020

Promotion of cutaneous penetration of nifedipine for nanoemulsion
Provedor de dados: 60
Autores: Arantes,Paula de Oliveira; Santos,Quesia Nery dos; Freitas,Zaida Maria Faria de; Pyrrho,Alexandre dos Santos; Cerqueira-Coutinho,Cristal; Villa,Ana Lucia Vazquez; Santos,Elisabete Pereira dos; Ricci-Júnior,Eduardo.
ABSTRACT This article reports the development and characterization of a nanoemulsion (NE) able to improve the cutaneous penetration of nifedipine. NE with nifedipine was development and characterized, presenting droplet size of 20 nm with low polydispersity index (IP&lt;0.1), spherical shape without aggregation, pH compatible with typical skin levels and stability evaluated by seven months. In the permeation studies, a classical formulation based in an oil/water cream containing nifedipine was used for comparison with NE. Nanoemulsion promoted and improved the retention of nifedipine in the epidermis and dermis in relation to classical formulation. This promoting effect is related to the nanometric size of the droplets of the NE (20 nm), which give him a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Topical nifedipine/cutaneous permeation; Nanoemulsion/characterization; Stability; Cutaneous permeation/study; Healing of wounds.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200628

Prevalence of potential drug-drug interactions in the intensive care unit of a Brazilian teaching hospital
Provedor de dados: 60
Autores: Rodrigues,Aline Teotonio; Stahlschmidt,Rebeca; Granja,Silvia; Pilger,Diogo; Falcão,Antonio Luis Eiras; Mazzola,Priscila Gava.
Abstract Patients in intensive care unit are prescribed large numbers of drugs, highlighting the need to study potential Drug-Drug Interactions in this environment. The aim of this study was to delineate the prevalence and risk of potential drug-drug interactions between medications administered to patients in an ICU. This cross-sectional observational study was conducted during 12 months, in an adult ICU of a teaching hospital. Inclusion criteria were: prescriptions with 2 or more drugs of patients admitted to the ICU for &gt; 24 hours and age of ≥18 years. Potential Drug-Drug Interactions were quantified and classified through MicromedexTM database. The 369 prescriptions included in this study had 205 different drugs, with an average of 13.04 ± 4.26...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potential drug-drug interactions; Intensive care unit; Patient safety; University hospitals.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100624

Wound-healing evaluation of ointment from Stryphnodendron adstringens (barbatimão) in rat skin
Provedor de dados: 60
Autores: Hernandes,Luzmarina; Pereira,Luciene Marques da Silva; Palazzo,Fabiana; Mello,João Carlos Palazzo de.
This study evaluated the cicatrizant effect of an ointment containing 1% of the ethyl-acetate fraction extracted from the stem bark of "barbatimão" (Stryphnodendron adstringens), in wounds made in the skin of rats, after 4, 7 and 10 days of treatment. Control wounds were treated with a base ointment without extract. The proliferation of keratinocytes in the area of reepithelialization was evaluated by counting the number of epithelial cells that were blocked in metaphase by vincristine sulfate. The length of the reepithelialized margin and the contraction of the wound were measured. Topical application of the "barbatimão" ointment stimulated proliferation of the keratinocytes, but had no effect on the length of the epithelium or on the contraction of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stryphnodendron adstringens/pharmacognosy; Stryphnodendron adstringens/cicatrizant effect; "Barbatimão"/pharmacognosy; Wound/healing; Wound/experimental study; Reepithelialization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300005

How much to invest in glycemic control of a patient with diabetes mellitus type 2? A constant dilemma for the Brazilian Public Health System (SUS)
Provedor de dados: 60
Autores: Gonçalves,Ana Carolina de Oliveira; Cazarim,Maurilio de Souza; Sanches,Cristina; Pereira,Leonardo Régis Leira; Baldoni,André de Oliveira.
Diabetes mellitus type 2 (DM2) affects millions of people worldwide and causes several complications for the patient, consuming large sums of financial resources from the health services. This study aims to estimate the financial investment of DM2 treatment for glycemic control of the patient, from the point of view of the municipal Public Health System (SUS). The Delphi technique was used to validate the opinion of a team of judges, specialists in DM2, and health service managers, on the investment necessary for glycemic control of patients with DM2 through the application of questionnaires. In order for the patient to achieve glycated hemoglobin (A1c) &lt; 7%, an investment of US$ 2,419.06 (value/patient/year) is necessary. As the value of A1c increases,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Pharmacoeconomics; Health care cost.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100522

Critical analysis: use of polymerase chain reaction to diagnose leprosy
Provedor de dados: 60
Autores: Maltempe,Flaviane Granero; Baldin,Vanessa Pietrowski; Lopes,Mariana Aparecida; Siqueira,Vera Lúcia Dias; Scodro,Regiane Bertin de Lima; Cardoso,Rosilene Fressatti; Caleffi-Ferracioli,Katiany Rizzieri.
ABSTRACT Leprosy is a neglected tropical disease and an important public health problem, especially in developing countries. It is a chronic infectious disease that is caused by Mycobacterium leprae, which has a predilection for the skin and peripheral nerves. Although it has low sensitivity, slit-skin smear (SSS) remains the conventional auxiliary laboratory technique for the clinical diagnosis of leprosy. Polymerase chain reaction (PCR) is a molecular biology technique that holds promise as a simple and sensitive diagnostic tool. In the present study, the performance of two PCR methods, using different targets, PCR-LP and PCR-P, were compared with SSS with regard to leprosy diagnosis in a reference laboratory. M. leprae DNA was extracted from 106 lymph...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leprosy/clinical diagnosis; Polymerase chain reaction/clinical diagnosis use; Mycobacterium leprae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100018

Level of satisfaction of clients of public pharmacies dispensing high-cost drugs in Espírito Santo, Brazil
Provedor de dados: 60
Autores: Cassaro,Karla Oliveira dos Santos; Heringer,Otávio Arruda; Fronza,Márcio; Lenz,Dominik; Endringer,Denise Coutinho; Andrade,Tadeu Uggere de.
ABSTRACT The aim of this study was to evaluate the level of satisfaction of pharmaceutical services and to determine the socioeconomic profile of public pharmacy clients. This is a cross-sectional observational study using a quantitative approach. The sample was calculated from the monthly number of patients seen in pharmacies and was stratified for each pharmacy. Data were analysed using SPSS 11.5 software and by observing the simple relative frequencies for qualitative variables. For the quantitative variables, the analysis of variance (ANOVA) and post-hoc Fisher tests were performed. Data are expressed as mean ± standard deviation (SD). The results show that, in general, patients at "Farmácias cidadãs" are satisfied with the services. However, when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Public Pharmacy; Unified Health System; Pharmaceutical Services.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100011

Phenobarbital loaded microemulsion: development, kinetic release and quality control
Provedor de dados: 60
Autores: Figueiredo,Kayo Alves; Neves,Jamilly Kelly Oliveira; Silva,José Alexsandro da; Freitas,Rivelilson Mendes de; Carvalho,André Luis Menezes.
ABSTRACT This study aimed to obtain and characterize a microemulsion (ME) containing phenobarbital (PB). The PB was incorporated in the proportion of 5% and 10% in a microemulsion system containing Labrasol(r), ethanol, isopropyl myristate and purified water. The physicochemical characterization was performed and the primary stability of the ME was evaluated. An analytical method was developed using spectrophotometry in UV  = 242 nm. The kinetics of the in vitro release (Franz model) of the ME and the emulsion (EM) containing PB was evaluated. The incorporation of PB into ME at concentrations of 5 and 10% did not change pH and resistance to centrifugation. There was an increase in particle size, a decrease of conductivity and a change in the refractive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microemulsion/physicochemical characterization; Microemulsion/In vitro release; Phenobarbital/microemulsion system/evaluation; Nanotechnology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200251

Pharmaceutical ethnobotany in the Mahabad (West Azerbaijan) biosphere reserve: ethno-pharmaceutical formulations, nutraceutical uses and quantitative aspects
Provedor de dados: 60
Autores: Jafarirad,Saeed; Rasoulpour,Ibrahim.
This study endeavors to overcome the limits of an orally transmitted pharmacopoeia, and tries to utilize the large ethnobotany patrimony of the area to investigate the biological diversity. Thirty-five traditional practitioners from dissimilar ethnic groups including traditional health practitioners (THPs) and indigenous people were interviewed. A total of 35 species of plants, belonging to 20 families were recognized for the treatment of more than 26 types of ailments. Informant consensus factor (FIC) values of this study reflected the high agreement in the use of plants in the treatment of gastro-intestinal complaints, infectious, parasitic diseases and constipation among the informants. Constipation had the highest use-reports and 8 species of plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical Ethnobotany; Ethno-Medicinal Knowledge; Indigenous people; Nutraceutical aspects; Ethnobotany/trends; Biosphere/analysis; Medicine tradicional/utilization; Ethnopharmacology; Plants/drug effects; Phytochemicals/pharmacology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100501

Use of an indicator to evaluate physician adherence to prescription guidelines for the treatment of heart failure
Provedor de dados: 60
Autores: Oliveira,Márcio Galvão; Passos,Luiz Carlos; Santos Júnior,Edval Gomes; Barbosa,Andrea Cristina; Santos,Djanilson Barbosa.
The aim of this study was to use indicators to evaluate physician adherence to prescription guidelines for heart failure treatment in a university hospital. This was a prospective cohort study conducted in a university hospital. The information collected at the time of patient admission, including therapeutic indication, absolute contra indications and intolerance, was utilised for the formulation of a guideline adherence indicator (GAI). This indicator was calculated as follows: (the number of patients who used the medication/the number of eligible patients) x 100. The percentage of eligible patients was calculated using the following formula: (the number of eligible patients/the total number patients) x 100. The GAI was applied to a population of 53...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure/treatment; Health care; Clinical medicine/practice guidelines; Medicines/use; Medicines/prescriptions.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400023

Average bioequivalence of single 500 mg doses of two oral formulations of levofloxacin: a randomized, open-label, two-period crossover study in healthy adult Brazilian volunteers
Provedor de dados: 60
Autores: Kano,Eunice Kazue; Koono,Eunice Emiko Mori; Schramm,Simone Grigoleto; Serra,Cristina Helena dos Reis; Abib Junior,Eduardo; Pereira,Renata; Freitas,Márcia Sayuri Takamatsu; Iecco,Maria Cristina; Porta,Valentina.
Average bioequivalence of two 500 mg levofloxacin formulations available in Brazil, Tavanic(c) (Sanofi-Aventis Farmacêutica Ltda, Brazil, reference product) and Levaquin(c) (Janssen-Cilag Farmacêutica Ltda, Brazil, test product) was evaluated by means of a randomized, open-label, 2-way crossover study performed in 26 healthy Brazilian volunteers under fasting conditions. A single dose of 500 mg levofloxacin tablets was orally administered, and blood samples were collected over a period of 48 hours. Levofloxacin plasmatic concentrations were determined using a validated HPLC method. Pharmacokinetic parameters Cmax, Tmax, Kel, T1/2el, AUC0-t and AUC0-inf were calculated using noncompartmental analysis. Bioequivalence was determined by calculating 90%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Levofloxacin/bioequivalence; Pharmacokinetics; High-performance liquid chromatography/qualitative analysis; Tavanic(c)/bioequivalence; Levaquin(c)/bioequivalence.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100203

Potential of Pandanus odoratissimus as a CNS depressant in Swiss albino mice
Provedor de dados: 60
Autores: Raju,Sama; Subbaiah,Narra Venkata; Reddy,Kandukuri Sreeramulu; Das,Amit; Murugan,Krishnan Bala.
In this study, several neuropharmacological effects of methanolic leaf extract of Pandanus odoratissimus (PO) (family; Pandanaceae) were studied in albino mice using various experimental models. The effect of PO on the CNS was studied by using different neuropharmacological paradigms including spontaneous motor activity, rota-rod performance and potentiation of Pentobarbital sodium sleeping time in albino mice. Preliminary phytochemical evaluation and acute toxicity studies were also carried out where LD50 &gt;2000 mg/kg was considered non-toxic through acute exposure in rats by the oral route. The methanolic leaf extract (50,100 and 200 mg/kg i. p.) produced a reduction in spontaneous motor activity, motor coordination and prolonged Pentobarbital sodium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pandanus odoratissimus/pharmacognosy; CNS-depressant action/experimental study; Spontaneous motor activity; Rota-rod performance; Sodium pentobarbital/potentiation of sleeping time; Pandanus odoratissimus/farmacognosia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300022

Overview of P-glycoprotein inhibitors: a rational outlook
Provedor de dados: 60
Autores: Srivalli,Kale Mohana Raghava; Lakshmi,P. K.
P-glycoprotein (P-gp), a transmembrane permeability glycoprotein, is a member of ATP binding cassette (ABC) super family that functions specifically as a carrier mediated primary active efflux transporter. It is widely distributed throughout the body and has a diverse range of substrates. Several vital therapeutic agents are substrates to P-gp and their bioavailability is lowered or a resistance is induced because of the protein efflux. Hence P-gp inhibitors were explored for overcoming multidrug resistance and poor bioavailability problems of the therapeutic P-gp substrates. The sensitivity of drug moieties to P-gp and vice versa can be established by various experimental models in silico, in vitro and in vivo. Ever since the discovery of P-gp, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-glycoprotein/inhibitors; Multidrug resistence; Cluster of differentiation 243; Sphingolipids; Competitive inhibitors.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300002

Formulation and evaluation of orally disintegrating clopidogrel tablets
Provedor de dados: 60
Autores: Mahrous,Gamal Mohamed; Kassem,Mohamed Gabr; Ibrahim,Mohamed Abbas; Auda,Sayed Hassan.
ABSTRACT Recent advances in drug delivery systems have aimed to achieve better patient compliance. One of these advances is the formulation of orally disintegrating tablets (ODTs) that dissolve instantaneously, releasing drugs within a few seconds without the need of water. The main objective of this paper was to prepare and develop ODTs of clopidogrel. The ODTs were prepared by direct compression. The effect of three superdisintegrants, namely crospovidone, croscarmellose sodium, and sodium starch glycolate, using three different disintegration times on the dissolution rate was investigated. The prepared tablets were evaluated for hardness, friability, disintegration time and in vitro drug release. Furthermore, the interaction of clopidogrel with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clopidogrel/orally disintegrating tablets; Superdisintegrants; Orally disintegrating tablets/formulation; Orally disintegrating tablets/evaluation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200309

Synthetic phosphoethanolamine: the state of the art of scientific production
Provedor de dados: 60
Autores: Anastácio,Lucas de Barros; Delmaschio,Camila Rocha; Oliveira,Danielle Aparecida; Chequer,Farah Maria Drumond.
ABSTRACT Cancer is a multifactorial disease and a serious public health problem. Currently, alternative drug treatments for cancer are actively being sought, which is the case of synthetic phosphoethanolamine (PHOS-S), a compound that could possibly have anticarcinogenic effects. To analyze the available scientific evidence to evaluate the anticarcinogenic effects of in vivo and in vitro PHOS-S. A systematic literature review of scientific articles aimed at evaluating the anticarcinogenic potential of PHOS-S, in vivo and in vitro, using the databases PubMed, ScienceDirect, SciElo, CAPES Portal and LILACS. The selected papers suggest a possible anticarcinogenic effect of PHOS-S by inhibiting tumor growth by inducing apoptosis and cell cycle blockade as well...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synthetic phosphoethanolamine/effects/in vivo/in vitro; Antineoplastic Agent; Cancer.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400402

Development of a new HPLC method for in vitro and in vivo studies of haloperidol in solid lipid nanoparticles
Provedor de dados: 60
Autores: Yasir,Mohd; Sara,Udai Vir Singh; Som,Iti.
ABSTRACT A simple and sensitive HPLC method was developed and validated for the quantification of haloperidol in solid lipid nanoparticles (SLNs). The developed method was used for detection of shelf life of haloperidol in SLNs. Calibration curve of haloperidol was also constructed in rat plasma using loratidine as internal standard. In vivo studies were performed on rats and concentration of haloperidol in brain and blood was measured for the determination of various pharmacokinetic and hence brain targeting parameters. Chromatogram separation was achieved using C18 column as stationary phase. The mobile phase consisted of 100 mM/L potassium dihydrogen phosphate-acetonitrile-TEA (10:90:0.1, v/v/v) and the pH was adjusted with o-phosphoric acid to 3.5....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haloperidol/quantification; Haloperidol/pharmacokinetic; Haloperidol/in vitro study; Haloperidol/in vivo study; High performance liquid chromatography/solid lipid nanoparticles; Plasma; Validation; Brain targeting.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200623

Marsdenia tenacissima extract alters crucial metabolites in cancer, determined by 1 H NMR based metabolomics approach
Provedor de dados: 60
Autores: Roy,Debmalya; Chen,Cheng; Wang,Jun-song; Yuan,Shengtao; Sun,Li.
ABSTRACT Altered metabolites level in the biosystems, is the potential cause of cancer, the primary reason of alteration of metabolism is change in nutrient consumption and waste excretion, as a result genetic mutation leads to cancer initiation and progression. Aberration of specific metabolites such as fumarate, succinate, 2-hydroxyglutarate may alter cell signaling. We collected liver and kidney samples and prepared for 1H NMR analysis, then executed NMR spectroscopy. We used a set of domestic R scripts to perform an unsupervised principal component analysis (PCA) and a supervised orthogonal signal correction partial least-squares discriminant analysis (OSC-PLS-DA). It signifies class discrimination for getting a clear separation, whereas PCA scores...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marsdenia tenacissima extract (MTE); MDA-MB-231; 1H NMR; Metabolomics.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200603

Effect of silicification on the water sorption properties of microcrystalline cellulose II
Provedor de dados: 60
Autores: Camargo,John Rojas; Garcia,Carlos Andrés Ortiz.
The aim of this study was to investigate the water sorption behavior of cellulose II:SiO2 composites and to determine the influence of silicification on this property. These composites were prepared by spray-drying at a cellulose II:SiO2 ratio of 98:2, 95:5, 90:10 and 80:20. The nonlinear models of Guggenheim-Anderson-de Boer (GAB), Generalized D'Arcy and Watt (GDW) and Hailwood &amp;amp; Horrobin (HH), were used for the characterization and analysis of the isotherms. The infrared and powder X-rays characterization showed no signs of chemical modification or change in the polymorphic form of cellulose II by SiO2. The parameters derived from these models indicated that only a 20% level of silicification was able to hinder the water sorption properties of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulose II; Silicon dioxide; Silicification; Water sorption isotherms; Disintegration.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100016

Nitric oxide releasing-dendrimers: an overview
Provedor de dados: 60
Autores: Roveda Júnior,Antonio Carlos; Franco,Douglas Wagner.
Platforms able to storage, release or scavenge NO in a controlled and specific manner is interesting for biological applications. Among the possible matrices for these purposes, dendrimers are excellent candidates for that. These molecules have been used as drug delivery systems and exhibit interesting properties, like the possibility to perform chemical modifications on dendrimers surface, the capacity of storage high concentrations of compounds of interest in the same molecule and the ability to improve the solubility and the biocompatibility of the compounds bonded to it. This review emphasizes the recent progress in the development and in the biological applications of different NO-releasing dendrimers and the nitric oxide release pathways in these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimer; Nitric oxide; Nitrosyl complexes; Diazeniumdiolate; Nitrosothiol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700002

Physical chemistry evaluation of stability, spreadability, in vitro antioxidant, and photo-protective capacities of topical formulations containing Calendula officinalis L. leaf extract
Provedor de dados: 60
Autores: Deuschle,Viviane Cecília Kessler Nunes; Deuschle,Regis Augusto Norbert; Bortoluzzi,Mariana Rocha; Athayde,Margareth Linde.
Calendula is used widely in cosmetic formulations that present phenolic compounds in their chemical constitution. The objective of our research was to develop and evaluate the stability of topical formulations containing 5% hydro-ethanolic extract of calendula leaves, including spreadability, and in vitro photo-protective, and antioxidant capacity. To evaluate the stability, we used organoleptic characteristics, pH, and viscosity parameters. Antioxidant capacity was measured by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, and the photo-protective capacity by SPF spectrophotometric measure. All formulations were stable. The calendula extract formulations in gel and cream showed no significant variations in pH, and the cream formulations presented lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calendula officinalis L./pharmacognosy; Topical formulations/in vitro evaluation; Calendula/topical formulations/in vitro evaluation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100063

Medicinal plants with teratogenic potential: current considerations
Provedor de dados: 60
Autores: Costa,Kassiane Cristine da Silva; Bezerra,Suzana Barbosa; Norte,Clevanice Moreira; Nunes,Luciana Macatrão Nogueira; Olinda,Tiago Moreira de.
The aim of this study was to present the implications of the use of herbs during pregnancy, pointing out those that should be avoided during this condition because of their abortifacient and/or teratogenic potential. We carried out searches in the databases ScienceDirect, Scielo and Google Scholar, adopting as criteria for inclusion: book chapters and/or complete articles (with abstract), available in English, Portuguese or Spanish, published from 1996 to in 2011. After a pre-selection of 83 articles, 49 bibliographies were used in the manufacturing end of the article, where 25 were from the Scielo database, 18 from ScienceDirect and 6 from Google Scholar. From the articles studied, we identified the four most commonly used plants as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants/undue use; Medicinal plants/pregnancy; Medicinal plants/teratogenic activity; Medicinal plants/emmenagogue; Medicinal plants/abortifacient agents; Natural products/adverse effects; Herbal medicines/adverse effects.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300009

Development of fast dispersing tablets of nebivolol: experimental and computational approaches to study formulation characteristics
Provedor de dados: 60
Autores: Jayaramu,Rajamma Abburu; Boregowda,Sateesha Shivally; Varma,Addanki Rahul Deva; Kalegowda,Chandan.
Formulation of FDT (fast dispersing tablets) of nebivolol was optimized and evaluated using simplex lattice design (SLD). The influence of type and concentration of three disintegrants viz.,Ac-Di-Sol, Primojel and Polyplasdone XL on hardness, friability and disintegration time of tablet was studied. Response surface plot and the polynomial equations were used to evaluate influence of polymer on the tablet properties. Results were statistically analyzed using ANOVA, and a p &lt; 0.05 was considered statistically significant. Results reveal that fibrous integrity and optimal degree of substitution in Primojel and Ac-Di-Sol are mainly responsible for the hardness of the tablet. Use of Polyplasdone in higher percentage in tablet formulation may result in high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nebivolol/fast dispersing tablets; Simplex lattice design; Tablets/fast dispersing/swelling capacity; Tablets/fast dispersing/hydration capacity; Tablets/fast dispersing/disintegration time.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400956

Effect of a Pharmaceutical Care Program on quality of life and satisfaction with pharmacy services in patients with type 2 diabetes mellitus
Provedor de dados: 60
Autores: Correr,Cassyano Januário; Pontarolo,Roberto; Souza,Rodrigo Augusto de Paula e; Venson,Rafael; Melchiors,Ana Carolina; Wiens,Astrid.
The aim is to evaluate the humanistic outcomes in type 2 diabetic patients by the adoption of pharmacotherapy follow-up in community pharmacies. Controlled, non-randomized, 12-months trial; n=161 patients distributed into control and intervention groups; 6 community pharmacies involved, all in the Curitiba city region, in the state of Paraná were used. The health-related quality of life (HRQoL) and the satisfaction index were determined using both the DQOL assessment tool, which measures HRQoL, and the satisfaction evaluation tool (QSSF). Interventions on 119 negative therapeutic outcomes were done (2.3/patient [SD=1.6]); the most commonly found problems were related to ineffectiveness of pharmacotherapy (68.1%). The Intervention-Group showed a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus/type 2/pharmaceutical care; Pharmaceutical care; Community pharmacy; Pharmacists/community action.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400027

Influence of papain in biofilm formed by methicillin-resistant Staphylococcus epidermidis and methicillin-resistant Staphylococcus haemolyticus isolates
Provedor de dados: 60
Autores: Oliveira,Hanna Lara da Cruz Dinéas de; Fleming,Maria Emília Castro Kling; Silva,Patrícia Vollu; Paula,Geraldo Renato de; Futuro,Débora Omena; Velarde,Guillermo Coca; Esper,Luciana Maria Ramires; Teixeira,Lenise Arneiro.
Methicillin-resistant Staphylococcus epidermidis (MRSE) and methicillin-resistant Staphylococcus haemolyticus (MRSHa) are important coagulase-negative staphylococci. They are often isolated from bacteremia in humans mainly due to their ability to form biofilm on the surfaces of medical devices. Papain is a complex mixture of proteolytic enzymes and peroxidases extracted from the latex of Carica papaya and it is recognized by accelerating the healing process of wounds. This study aimed to evaluate the ability of the MRSE and MRSHa isolates to produce biofilms. Besides this, the ability of papain to inhibit the formation of biofilms or to disrupt the ones already formed by those bacteria was analyzed. Thirty MRSHa and 30 MRSE were isolated from bacteremia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus epidermidis/methicillin-resistant; Staphylococcus haemolyticus/methicillin-resistant; Biofilm/production; Papain/reducing biofilm.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200261

Aplicação de métodos de biofísica no estudo da eficácia de produtos dermocosméticos
Provedor de dados: 60
Autores: Gonçalves,Gisele Mara Silva; Campos,Patrícia Maria Berardo Gonçalves Maia.
O trabalho aborda a aplicação dos métodos de biofísica no estudo de eficácia de produtos dermocosméticos. A qualidade de um produto cosmético engloba a segurança de seu uso, a estabilidade da formulação, o aspecto, o sensorial e a sua eficácia. As técnicas de biofísica têm sido amplamente empregadas na avaliação do efeito de formulações dermocosméticas, principalmente devido ao fato de possibilitarem a avaliação de produtos em suas reais condições de uso, ou seja, na pele humana. A comprovação dos efeitos de produtos cosméticos e a elucidação de seu mecanismo de ação por meio de protocolos experimentais devidamente elaborados, que utilizem as técnicas de biofísica da pele, dentre outras, tornam-se de fundamental importância e, assim, a serem amplamente...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Produtos dermocosméticos/controle de qualidade; Pele/biofísica; Pele/estudo; Biofísica/técnicas; Biofísica/aplicação em cosmetologia; Cosméticos/eficácia; Dermocosmetic products/quality control; Skin/biophysics; Skin/study; Biophysics/techniques; Biophysics/cosmetologic application; Cosmetics/effectiveness.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100002

In vivo and in vitro genotoxicity studies of aqueous extract of Xanthium spinosum
Provedor de dados: 60
Autores: Güez,Camila Martins; Waczuk,Emily Pansera; Pereira,Karina Braccini; Querol,Marcus Vinícius Morini; Rocha,João Batista Teixeira da; Oliveira,Luís Flávio Souza de.
The use of plants as a source of palliative or cure for pathological conditions is quite common worldwide. Xanthium spinosum (Asteraceae), popularly known in Brazil as 'espinho de carneiro', is an annual weed from South America, which has been used by empiric medicine to treat neoplasias. Owing to the extensive use of the above-mentioned plant and to the lack of reports about the real effects of its infusion, current study evaluated the genotoxic potential of its aqueous extract at concentrations 0.02 g L-1, 0.1 g L-1 and 0.2 g L-1 by fish micronucleus test and by comet human leukocytes assay. The micronucleus test featured at least 50 cells with micronuclei to every 2,000 cells scored, as a mutagenic parameter. The comet assay was used as a parameter for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xanthium spinosum/pharmacognosy; Xanthium spinosum/aqueous extract; Xanthium spinosum/genotoxicity; Fish micronucleus assay; Comet assay.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300013

Reduction of cardiovascular risk in patients with metabolic syndrome in a community health center after a pharmaceutical care program of pharmacotherapy follow-up
Provedor de dados: 60
Autores: Plaster,Camila Pedro; Melo,Danilo Travassos; Boldt,Veraci; Cassaro,Karla Oliveira dos Santos; Lessa,Fernanda Campos Rosetti; Boëchat,Giovanna Assis Pererira; Bissoli,Nazaré Souza; Andrade,Tadeu Uggere de.
The objective of this study was to determine the impact of a pharmaceutical care (PC) program in a sample of public outpatients with metabolic syndrome (MS) who were being treated in Brazil's health system; the patients were randomized into PC or standard care. The pharmacotherapy follow-up (PF) was performed in a total of 120 patients with type 2 diabetes for 6 months. Adherence to treatment (measured with the Morisky test), negative outcomes associated with medication (NOM) and anthropometric and biochemical parameters were measured before and after PF. The Framingham scoring method was used to estimate changes in 10-year coronary heart disease risk scores in all patients. Ninety-six of 120 patients had characteristics of MS and were randomized into two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical service; Metabolic syndrome/cardiovascular risk; Drug therapy follow-up; Medicines/associated negative outcomes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300010

Pharmacobotanic characterization of young stems and stem barks of Rauvolfia sellowii Müll. Arg., Apocynaceae
Provedor de dados: 60
Autores: Baratto,Leopoldo Clemente; Duarte,Márcia do Rocio; Santos,Cid Aimbiré de Moraes.
Rauvolfia sellowii Müll. Arg. (Apocynaceae), a Brazilian native tree rich in indole alkaloids, is known as "pau-pra-tudo" and popularly used as hypocholesterolemic, hypoglycemic and antihypertensive. The aim of this work was to study the anatomy of the young stems and stem barks of this medicinal plant, in order to contribute to the identification of the species as a drug. The plant material was fixed and prepared according to standard microtechniques. The young stems have remaining epidermis, but a suberified peridermis is evident. The phellogen is located in the cortical region, forming suber externally. Underneath the phellogen, lies the phelloderm and collenchymatic region. In the cortex, there are numerous laticifers and some fibers. There is an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rauvolfia sellowii/pharmacognosy; Apocynaceae/pharmacognosy; Medicinal plants; Vegetal anatomy; Internal phloem; Laticiferous ducts; Calcium oxalate crystals.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300020

Systematic review protocol: following PRISMA guide from Cochrane to generate evidence as treatment effect of pharmaceutical care for hypertension in primary care
Provedor de dados: 60
Autores: Cazarim,Maurílio de Souza; Reis,Tiago Marques dos; Cruz-Cazarim,Estael Luzia Coelho da; Pereira,Leonardo Régis Leira.
Pharmaceutical care (PC) is in the implementation process in Brazil and Latin America. Synthesis of evidence has been requested for monitoring and evaluating the process regarding the treatment effect. The objective is to build and disseminate a systematic review protocol to make a standard for updating results from pharmaceutical care for hypertension and for other diseases. This is a protocol for systematic review studies regarding a real example of a protocol reasoned in pharmaceutical care for hypertension in primary care. This protocol was delineated grounded in the Cochrane Handbook. Descriptors and words were defined using MeSH (Medical Subject Headings), DeCS (Descriptors in Health Sciences) and Emtree thesaurus, and the search was performed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical services; Hypertension; Primary health care; Review literature as topic; Evidence-based medicine.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100620

Widespread use of anti-retroviral therapy elevated the prevalence of dyslipidemia in HIV-infected Chinese patients
Provedor de dados: 60
Autores: Ye,He; Hong,Yu Min; Jing,Song Xiao; Hui,Yang; Juan,Zhang Xiao; Wei,Zhao Yan.
With the widespread use of high-efficiency antiretroviral therapy (HAART), the life expectancy of HIV-infected patients has been significantly extended. However, the metabolic complications among HIV-infected patients treated with HAART have become the most common problem in the world. It is very important to explore the incidence of dyslipidaemia and studies on the role of potential risk factors in HIV-infected Chinese patients treated with HAART are sparse. Therefore, we designed current study, to investigate the effects of therapeutic intervention and continuous information support on the lifestyle of HIV/AIDS patients with dyslipidaemia. Three hundred and six HIV/AIDS patients admitted to the AIDS clinic in Beijing from January 2016 to January 2017...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic Complications; Lipid; Dyslipidaemia; HIV; HAART.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100636

Development and validation of an HPLC method for the simultaneous determination of artesunate and mefloquine hydrochloride in fixed-dose combination tablets
Provedor de dados: 60
Autores: Nogueira,Fernando Henrique Andrade; Reis,Naialy Fernandes Araújo; Chellini,Paula Rocha; César,Isabela da Costa; Pianetti,Gerson Antônio.
The present study developed and validated an HPLC method for the simultaneous determination of artesunate (AS) and mefloquine hydrochloride (MQ) in fixed-dose combination tablets, according to ICH guidelines. The chromatographic separation was carried out on an XBridge C18 (250 x 4.6 mm i.d., 5 µm particle size, Waters) analytical column. The mobile phase included a 0.05 M monobasic potassium phosphate buffer (pH adjusted to 3.0 with phosphoric acid) and acetonitrile (50 + 50, v/v). The flow rate was 1.0 mL/min, and the run time was 13 minutes. A dual-wavelength approach was employed: AS detection was performed at 210 nm and MQ was detected at 283 nm, using a diode array detector. Stability of sample solutions was evaluated for 8 hours after preparation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artesunate/determination; Mefloquine hydrochloride/determination; High performance liquid chromatography/qualitative analysis; Fixed-dose combination tablets/qualitative analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400024

Anti-Sporothrix brasiliensis activity of different pyrazinoic acid prodrugs: a repurposing evaluation
Provedor de dados: 60
Autores: Waller,Stefanie Bressan; Nakasu,Ceres; Silva,Anna Luiza; Faria,Renata Osório de; Fernandes,João Paulo dos Santos; Cleff,Marlete Brum.
From drug repurposing studies, this work aimed to evaluate the activity of different pyrazinoic acid (POA) derivatives against Sporothrix brasiliensis. The POA esters were prepared and characterized as previously reported by classical esterification reactions, with good to excellent yields. Sporothrix brasiliensis isolates from cats (n=6) and standard strains of S. brasiliensis and S. schenckii were used to assess the antifungal activity of the POA derivatives through broth microdilution assay (CLSI M38-A2). Among the tested compounds, molecules 3 and 4 showed fungistatic and fungicidal activities against all Sporothrix spp. strains, and the obtained MIC and MFC values ranged from 2.12 to 4.24 mg/mL and from 1.29 to 5.15 mg/mL, respectively. Compound 2 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sporotrichosis; Sporothrix schenckii complex; Itraconazole; Antifungal resistance; Prodrugs; Pyrazinoic acid derivatives.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400630

Protection effect of sanguinarine on whole-body exposure of X radiation in BALB/c mice
Provedor de dados: 60
Autores: Xu,Jia-Ying; Zhao,Lin; Chong,Yu; Jiao,Yang; Qin,Li-Qiang; Fan,Sai-Jun.
To investigate the effects of sanguinarine (SAN) on acute radiation induced injury in mice, 45 mice were randomly divided into control, 10 Gy and SAN+10 Gy groups. Mice in the 10 Gy and SAN+10 Gy groups were exposed to single X-ray radiation with an accumulated dose of 10 Gy. Mice in the SAN+10 Gy group were administered intraperitoneally with 2.5 mg/kg body weight of SAN before radiation. Five days after radiation exposure, 5 mice from each group were sacrificed and samples of the small intestine, lung, spleen and liver were fixed for histopathological examinations. Compared with the 10 Gy group, radiation sickness was obviously delayed or attenuated in the SAN+10 Gy group. Survival analysis showed a significant difference between 2 radiation groups...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sanguinarine/radioprotection; X-ray/radiation/effects; Radiation sickness; Radiation/survival analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100010

Standardization method for measurement of hydroxyurea by Ultra High Efficiency Liquid Chromatography in plasma of patients with sickle cell disease
Provedor de dados: 60
Autores: Elias,Darcielle Bruna Dias; Carvalho,Teresa Maria de Jesus Ponte; Soares,Janete Eliza de Sá; Gonçalves,Romélia Pinheiro.
Sickle cell anemia (SCA) is a recessively inherited disease characterized by chronic hemolytic anemia, chronic inflammation, and acute episodes of hemolysis. Hydroxyurea (HU) is widely used to increase the levels of fetal hemoglobin (HbF). The objective of this study was to standardize and validate a method for the quantification of HU in human plasma by using ultra high performance liquid chromatography (UPLC) in order to determine the plasma HU levels in adult patients with SCA who had been treated with HU. We used an analytical reverse phase column (Nucleosil C18) with a mobile phase consisting of acetonitrile/water (16.7/83.3). The retention times of HU, urea, and methylurea were 6.7, 7.7, and 11.4 min, respectively. All parameters of the validation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell anemia/therapeutic monitoring; Hydroxyurea/quantification in human plasma; Ultra High Efficiency Liquid Chromatography/quantitative analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300621

Synthesis and antibacterial activity of a series novel 5,7-diisoprenyloxyflavone derivatives
Provedor de dados: 60
Autores: Jin,Qing-Hao; Fu,Zhi-Yang; Xia,Ya-Nan; Liu,Bing-Yu; Jiang,Hai-Ying.
In the present study, a series of novel 5,7-diisoprenyloxyflavone derivatives were designed, synthesized, and evaluated for their antibacterial activity. Most of these compounds displayed significant antibacterial effects against Gram-positive bacteria, especially against strains of multidrug-resistant clinical isolates. Compounds 4c, 4g, 4i, 4j, 4k, 4l, 4n, 4q and 4t showed high levels of antimicrobial activity against Staphylococcus aureus RN4220 with minimum inhibitory concentrations of 4.0-20 µM. Compound 4k showed the most potent activity among these compounds against all multidrug-resistant clinical isolates tested. Unfortunately, none of the compounds were active against Gram-negative bacteria at the doses of 24-164 µM.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavonoids; Synthesis; Isoprenyloxyflavones; Antibacterial activity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100541

Hemathology
Provedor de dados: 60
Autores: Borelli,Primavera.
Tipo: Info:eu-repo/semantics/review
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100030

Evaluation of prescription indicators established by the WHO in Getúlio Vargas - RS
Provedor de dados: 60
Autores: Vooss,Andressa Tanise; Diefenthaeler,Helissara Silveira.
To evaluate the conditions of the services offered to the community regarding medications, the World Health Organization (WHO) has developed the Medication Use Indicators, among them Prescription indicators. The objective of this study was to evaluate drug prescriptions in a Basic Health Care Center in Getúlio Vargas - RS, according to the Prescription Indicators recommended by the WHO. The data collection was performed with the use of a form and the prescriptions for June / July and November / December 2008 were evaluated. The average number of drugs prescribed was 2.03, and 72.8% of the drugs were prescribed by generic name, 80.3% were on the list of essential drugs, 21.7% were antibiotics and 2.4% were injectable drugs. The results were in accordance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medications/rational use; Medications/prescription indicators; World Health Organization/medication use indicators.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200020

Evaluation of the use of topic nasal decongestants in university students from health sciences courses
Provedor de dados: 60
Autores: Lenz,Dominik; Cardoso,Kézia Santos; Bitti,Ana Carla Rangel; Andrade,Tadeu Uggere.
Nasal decongestants in Brazil are available without prescription. They are one of the most requested medications in Brazil. The use of nasal decongestants, however, may lead to serious consequences. For this study, university students majoring in the health field were evaluated in terms of their profile regarding the use of nasal decongestants. Additionally, health sciences seniors were evaluated regarding their level of knowledge concerning the correct and proper use of nasal decongestants. Among the 71.8% of respondents who had already used topic nasal decongestants, 64.3% had used this medication for at least 15 days and 64.6% had started using it due to nasal obstruction. The gathered data indicate that the pharmacist was the main health professional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nasal decongestans/use evaluation; Self-prescribed medication; Health education.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400013

Pharmaceutical care to pregnant women carrying human immunodeficiency virus
Provedor de dados: 60
Autores: Romeu,Geysa Aguiar; Paiva,Larissa Varela de; Fé,Mariana Mota Moura.
The purpose of this present study was to know the pharmacoepidemiological profile of pregnant women carrying HIV, the prevention actions against vertical transmission, and evaluate the service respecting the patients' level of satisfaction. It was developed a quali-quantitative longitudinal study, with the sample being composed by pregnant women carrying HIV attended at the Pharmaceutical Care Service of a public hospital of Fortaleza - state of Ceará, during the period between January and September, 2007. The data were collected by means of follow-up cards, patients' medical history and, finally, interviews to verify the users' level of satisfaction related to the service. Twenty seven pregnant and parturient women have participated in the study. The mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacoepidemiology; Pharmaceutical Care; HIV; Pregnant women/pharmacoepidemiological profile.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300026

Stryphnodendron adstringens (Mart.) Coville (Fabaceae) proanthocyanidins quantitation by RP-HPLC
Provedor de dados: 60
Autores: Nascimento,André Márcio do; Guedes,Priscila Tavares; Castilho,Rachel Oliveira; Vianna-Soares,Cristina Duarte.
Stryphnodendron adstringens (Mart.) Coville (barbatimão) is a tree belonging to the Fabaceae family, and it is commonly found in the southeastern Brazilian cerrado. The stem bark of this tree is traditionally used as an anti-inflammatory and antiseptic agent to treat leucorrhea and diarrhea, as well as to promote wound healing (owing to the presence of proanthocyanidins). Proanthocyanidins were obtained from the ethanolic extract of S. adstringens stem bark and assessed by reversed phase-high performance liquid chromatography with an ultraviolet/diode array detector. The identified compounds included gallic acid, catechin, gallocatechin (GC), epigallocatechin, and epigallocatechin gallate (EGCG). The selected markers, GC and EGCG, were simultaneously used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stryphnodendron adstringens/phytochemistry; Fabaceae/phytochemistry; Barbatimão/extrato vegetal; Barbatimão/evaluation; Proanthocyanidins/evaluation; RP-High performance liquid chromatography/análise quantitativa.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300016

Citral effect in male NMRI mice nonalcoholic steatosis model: assessing biochemical and histological parameters and PPARα gene expression
Provedor de dados: 60
Autores: Vaezi,Maryam; Yaghmaei,Parichehreh; Hayati-Roodbari,Nasim; Irani,Shiva; Ebrahim-Habibi,Azadeh.
Citral is a small molecule present in various citrus species, with reported anti-hyperlipidemic and anti-inflammation effects. Here, the effect of intraperitoneal (IP) administration of citral is evaluated in a mouse model of non-alcoholic steatosis. Male NMRI mice were divided into the following groups (n = 12): normal control group (NC) receiving a normal diet; high-fat emulsion group (HF) receiving high fat diet for four weeks; positive control group (C+) receiving HF diet for four weeks and then shifted to normal diet with IP-administered silymarin (80 mg/kg) for four weeks; sham group receiving HF diet for four weeks and then shifted to normal diet for four weeks; and EC1, EC2, and EC3 groups receiving HF diet for four weeks and then shifted to normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citral effects; Lemonal; Liver disease; Silymarin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300620

Preclinical evaluation of nephroprotective potential of a probiotic formulation LOBUN on Cyclosporine-A induced renal dysfunction in Wistar rats
Provedor de dados: 60
Autores: Venkateswarlu,Kambham; Heerasingh,Thakur; Babu,Chilamakuru Naresh; Triveni,Singirisetty; Manasa,Suroju; Babu,Thumati Nagendra Bhaskar.
ABSTRACT The aim of present study was to evaluate the nephroprotective effect of probiotic formulation LOBUN on Cyclosporine A (CsA) induced renal dysfunction in Wistar rats. CsA (20 mg/kg body weight s.c) was administered for 15 days to cause renal dysfunction in Wistar rats. The probiotic formulation LOBUN was administered with the dose of 500 mg/kg body weight (p.o) for twice (TGI) and thrice a day (TGII). The samples were analyzed for the parameters like blood urine nitrogen (BUN), serum creatinine, serum uric acid, total serum protein and urine proteins, urine potassium, urine sodium. The renal functional and histopathological studies revealed that the oral administration of probiotic formulation LOBUN has provided appreciable renoprotection and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclosporine-A; LOBUN/effects; LOBUN/effects/evaluation; Nephroprotective activity; Prebiotics; Probiotics; Chronic Kidney Disease/study.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200605

The effects of different doses of caffeine on performance, rating of perceived exertion and pain perception in teenagers female karate athletes
Provedor de dados: 60
Autores: Arazi,Hamid; Hoseinihaji,Marzieh; Eghbali,Ehsan.
ABSTRACT The present study set to examine the effects of different doses of caffeine on performance, rating of perceived exertion (RPE), and pain perception in female teenager athletes of karate. Ten female karate athletes (16.8±1.23 years; height 1.59±0.28 m; body-mass 57.73±8.33 kg; BMI 22.71±3.05 kg/m2) participated in the study. A double-blind, randomized, and crossover counterbalanced design was used. In three sessions (with an interval of seven days'), ten female karate athletes ingested low dose (2 mg/kg), moderate dose (5 mg/kg) caffeine, and placebo. Sixty minutes after consumption, they performed the tests as below: one repetition maximum and 60% of one repetition maximum in the leg press, explosive power test, and anaerobic RAST test. After the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caffeine/effects; Karate athletes/performance; Caffeine/rating of perceived exertion; Caffeine/performance; Caffeine/pain perception.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400685

Evaluation of antiarthritic activity of ginkgolic acid against Freund’s adjuvant induced arthritic rat model
Provedor de dados: 60
Autores: Zhang,Ziqiang; Wang,Xiaogang; Li,Pengshan; Bai,Minghua; Qi,Wenbing.
This study aimed to analyze the antiarthritic activity of ginkgolic acid against the Complete Freund’s Adjuvant (CFA)-induced arthritis in rats. Arthritis was induced through an intradermal injection of CFA (0.1 mL) at the right hind footpad of adult Wistar Albino rats. Ginkgolic acid was administered orally at doses of 25 mg/kg and 50 mg/kg, respectively, once daily via gavage for 25 days upon inducing arthritis. Indomethacin was administered orally at a dose of 3 mg/kg twice in a week which served as positive control group. The animals were sacrificed and subjected to biochemical and histopathological analysis upon completion of treatment. Ginkgolic acid was able to reverse the arthritic effect (p &lt; 0.01) induced by CFA in a dose dependent manner....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginkgolic acid; Complete Freund’s Adjuvant (CFA); Osteoarthritis; Antioxidant; Anti-inflammatory; Histopathology.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100617

Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract
Provedor de dados: 60
Autores: Mahmood,Tariq; Akhtar,Naveed; Khan,Barkat Ali; Rasul,Akhtar; Khan,Haji M. Shoaib.
Complex multiple emulsions have an excellent ability to fill large volumes of functional cosmetic agents. This study was aimed to encapsulate large volume of green tea in classical multiple emulsion and to compare its stability with a multiple emulsion without green tea extract. Multiple emulsions were developed using Cetyl dimethicone copolyol as lipophilic emulsifier and classic polysorbate-80 as hydrophilic emulsifier. Multiple emulsions were evaluated for various physicochemical aspects like conductivity, pH, microscopic analysis, rheology and these characteristics were followed for a period of 30 days in different storage conditions. In vitro and in vivo skin protection tests were also performed for both kinds of multiple emulsions i.e. with active...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmetics; Green tea; Multiple emulsion; Rheology; Skin; Stability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200016

Drugs obtained by biotechnology processing
Provedor de dados: 60
Autores: Almeida,Hugo; Amaral,Maria Helena; Lobão,Paulo.
In recent years, the number of drugs of biotechnological origin available for many different diseases has increased exponentially, including different types of cancer, diabetes mellitus, infectious diseases (e.g. AIDS Virus / HIV) as well as cardiovascular, neurological, respiratory, and autoimmune diseases, among others. The pharmaceutical industry has used different technologies to obtain new and promising active ingredients, as exemplified by the fermentation technique, recombinant DNA technique and the hybridoma technique. The expiry of the patents of the first drugs of biotechnological origin and the consequent emergence of biosimilar products, have posed various questions to health authorities worldwide regarding the definition, framework, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Recombinant DNA; Hybridoma technique; Bacteria; Antibiotics; Vaccines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200002

Comparison of the antioxidant potential of antiparkinsonian drugs in different in vitro models
Provedor de dados: 60
Autores: Farias,Carine Coneglian de; Bonifácio,Kamila Landucci; Matsumoto,Andressa Keiko; Higachi,Luciana; Casagrande,Rúbia; Moreira,Estefânia Gastaldello; Barbosa,Décio Sabbatini.
Parkinson's disease (PD) is characterized by progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta. Furthermore, oxidative stress plays a role in PD, causing or contributing to the neurodegenerative process. Currently PD has only symptomatic treatment and still nothing can be done to stop the degenerative process of the disease. This study aimed to comparatively evaluate the antioxidant capacity of pramipexole, selegeline and amantadine in different in vitrostudies and to offer possible explanations on the molecular antioxidant mechanisms of these drugs. In vitro, the antioxidant capacity of the drugs was assessed by the ability of antiparkinsonian drugs to decrease or scavenge ROS in the neutrophil respiratory burst,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson´s disease; Oxidative stress; Antiparkinsonian drugs/in vitrostudies; Antioxidants.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400819

Preparation and scale up of extended-release tablets of bromopride
Provedor de dados: 60
Autores: Ferreira,Guilherme Neves; Silva,Marcos Giovani Rodrigues; Fraga,Aline Guerra Manssour; Silva,Luiz Cláudio Rodrigues Pereira da; Lira,Luiz Marcelo; Rodrigues,Carlos Rangel; Castro,Helena Carla; Sousa,Valéria Pereira de; Cabral,Lucio Mendes.
Reproducibility of the tablet manufacturing process and control of its pharmaceutics properties depends on the optimization of formulation aspects and process parameters. Computer simulation such as Design of Experiments (DOE) can be used to scale up the production of this formulation, in particular for obtaining sustained-release tablets. Bromopride formulations are marketed in the form of extended-release pellets, which makes the product more expensive and difficult to manufacture. The aim of this study was to formulate new bromopride sustained release formulations as tablets, and to develop mathematical models to standardize the scale up of this formulation, controlling weight and hardness of the tablets during manufacture according to the USP 34th...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromopride/extended-release tablets/preparation; Design of experiments; Extended-release tablets/scale up obtention; Direct compression; Tablets/kinetics release.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200291

Influence of different formulation variables on the performance of transdermal drug delivery system containing tizanidine hydrochloride: in vitro and ex vivo evaluations
Provedor de dados: 60
Autores: Shabbir,Maryam; Sajid,Ali; Hamid,Irfan; Sharif,Ali; Akhtar,Muhammad Furqan; Raza,Moosa; Ahmed,Shoaib; Peerzada,Sohaib; Amin,Muhammad Umair.
The present study was aimed at preparation of transdermal patches of tizanidine HCl, evaluation of the effect of polymers on in vitro release pattern of the drug, and the effect of permeation enhancers on the penetration of the drug through the rabbit skin. Various proportions of hydrophilic (HPMC) and hydrophobic (Eudragit L-100) polymers were used with PEG 400 as film-forming agent, and Span 20 or DMSO as permeation enhancer. The formulations were assessed for physicochemical characteristics and in vitro drug release studies using USP paddle over disc method in phosphate buffered saline (pH 7.4) at 32.0±1°C. On the basis of in vitro studies and physicochemical evaluations, S03-A and S04-A were selected at Eudragit : HPMC ratios of 8 : 2 and 7 : 3,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ex-vivo permeation; Eudragit L100; Permeation enhances; Transdermal matrix patch; Monolithic system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400601

Methods of acute biological assays in guinea-pigs for the study of toxicity and innocuity of drugs and chemicals
Provedor de dados: 60
Autores: Ko,Gui Mi; Rosenkranz,Adela; Bertoncini,Clélia Rejane Antonio; Jurkiewicz,Neide Hyppolito; Franco,Mirian Ghiraldini; Jurkiewicz,Aron.
In this study, 602 samples were tested by the following assays performed at the animal facilities (Cedeme) of the Federal University of São Paulo (UNIFESP): 385 for dermal irritability, 90 for ocular irritability (discontinued in 1995), 31 for systemic toxicity by injection, 26 for oral acute toxicity, 15 for toxicity by intracutaneous injection, 15 for skin sensitization, 15 for toxicity of serum and vaccines for human use, 14 for toxicity by intramuscular implantation, 7 for pyrogens, 2 for acute dermal toxicity, and 2 for irritation of mucous membrane. The following agents were tested: cosmetics and related substances (42.0%), chemicals used in industry (32.9%), plastics, rubber, and other polymers (15.9%), agrotoxics (4.0%), medicines (2.7%), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute biological assays/methods; Cosmetics/toxicity; Cosmetics/experimental study; Cosmetics/innocuity; Drugs/toxicity; Drugs/experimental study; Drugs/innocuity; Chemical substances/toxicity; Chemical substances/experimental study; Dermal irritability/experimental study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200012

Equilibrium solubility study to determine fexofenadine hydrochloride BCS class and challenges in establishing conditions for dissolution profiles applied to suspension
Provedor de dados: 60
Autores: Rosa,Lorena de Sousa; Marques-Marinho,Flávia Dias; Braga,Silmara Leôncio; Souza,Jacqueline de.
The aim of this work was to perform solubility studies for fexofenadine hydrochloride and establish dissolution conditions for this drug in oral suspension dosage form. The solubility study was executed through the shake-flask method, below 37 ºC±1 ºC, at 100 rpm stirring for 12 h in three buffer solutions: hydrochloric acid pH 2.0, acetate pH 4.5 and phosphate pH 6.8. The dissolution test was developed in vessels containing 900 mL of the same buffer, employing the paddle apparatus in speed of 25 and 50 rpm, below 37 ºC±0.5 ºC. The drug was classified as low solubility according to the Biopharmaceutics Classification System, since the dose/solubility ratio was higher than 250 mL in all media tested (326.55 mL in buffer pH 2.0; 2,456.33 mL in buffer pH 4.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fexofenadine hydrochloride; HPLC method; Biopharmaceutics; Suspension; Dissolution profile.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100585

An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
Provedor de dados: 60
Autores: Silva,Pedro Henrique Reis da; Enéas,Paula Cristina Rezende; Fernandes,Christian; Pianetti,Gerson Antônio.
ABSTRACT Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanol:water (70:30) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2&gt;0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prednisone; Capsules; High performance liquid chromatography/validation; Linearity assessment.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200624

Application of biosynthesized ZnO nanoparticles on an electrochemical H 2 O 2 biosensor
Provedor de dados: 60
Autores: Zheng,Yuhong; Wang,Zhong; Peng,Feng; Fu,Li.
ABSTRACT ZnO nanoparticles (NPs) were synthesized via a green biochemical method using Corymbia citriodora leaf extract as a reducing and stabilizing agent. The biosynthesized ZnO NPs were characterized by SEM and XRD. An electrochemical H2O2 biosensor was fabricated by modification of a glassy carbon electrode using our proposed ZnO NPs. The electrochemical sensor showed excellent detection performance towards trace amounts of H2O2, demonstrating that it could potentially be used in clinical applications.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H2O2/electrochemical biosensor; ZnO/biosynthesis; Corymbia citriodora/stabilizing agent.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400781

Essential oil from Euphorbia esula inhibits proliferation and induces apoptosis in HepG2 cells via mitochondrial dysfunction
Provedor de dados: 60
Autores: Lv,Dan; Pan,Li-hong; Zhang,Ren; Yang,Jie; Chen,Hao; Wen,Yanzhang; Huang,Mi; Ma,Xinhua; Wang,Qiang; Yang,Xinzhou.
Hepatocellular carcinoma is one of the most prevalent malignancies and a leading cause of cancer-related mortality worldwide. However, the therapies to prevent hepatocellular carcinoma are still limited and the emergence of drug resistance leads to the development of new anti-cancer drugs and combinational chemotherapy regimens. Our study was aimed to explore the anticancer effects of the essential oil extract (EEEO) from Euphorbia esula which has been widely used in traditional Chinese folk medicine and possessed potential cytotoxic effects in several human tumor cells. However, the mechanisms of EEEO-induced anti-proliferation and apoptosis have not been completely elucidated. In this study, EEEO was prepared by hydro-distillation and the main chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbia esula; Hepatocellular carcinoma; Apoptosis; Reactive oxygen species; Mitochondrial pathway.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100506

Determination of cefotaxime, cefoperazone, ceftazidime and cefadroxil using surface plasmon resonance band of silver nanoparticles
Provedor de dados: 60
Autores: Salem,Hesham; Samir,Ebtihal.
The aim of this work is to evaluate simple, sensitive, effective and validated procedures for the determination of cefotaxime, cefoperazone, ceftazidime and cefadroxil. In this study, the methods based on the ability of the cited drugs to reduce Ag+ ions to silver nanoparticles (Ag-NPs) in the presence of Polyvinyl Pyrrolidone (PVP) as a stabilizing agent producing very intense surface plasmon resonance peak of Ag-NPs (λmax. = 410-430 nm). The plasmon absorbance of the Ag-NPs allows the quantitative spectrophotometric determination of the cited drugs. The calibration curves are linear with concentration ranges of 0.4-3.2, 1-8, 0.5-4.0 and 1.5-9.0 µg/mL for cefotaxime, cefoperazone, ceftazidime and cefadroxil, respectively. Apparent molar absorptivity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver nanoparticles; Cefotaxime; Cefoperazone; Ceftazidime; Cefadroxil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300617

Design, preparation and in vitro characterizations of fluconazole loaded nanostructured lipid carriers
Provedor de dados: 60
Autores: Fernandes,Amanda Velinna; Pydi,Chinna Raja; Verma,Ruchi; Jose,Jobin; Kumar,Lalit.
Present study was aimed to prepare and characterize fluconazole loaded nanostructured lipid carriers (FLZ-NLCs) for the treatment of fungal infections. Fungal infections are tremendously widespread and are the often faced dermatological condition worldwide. FLZ-NLCs was prepared by ultrasonication emulsion technique using stearic acid (SA) as solid lipid, castor oil as liquid lipid and tween 20 as a surfactant. The mean diameter of optimized FLZ-NLCs were found to be 359.15 ± 9.83 nm. The drug content and entrapment efficiency of NLCs was found to be 102.97 ± 7.45% and 87 ± 0.59%, respectively. In vitro drug release studies of FLZ-NLCs showed 37.34 ± 2.08% drug release over a period of 72 h. The above studies confirmed the prepared FLZ-NLCs may be useful...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluconazole; NLCs; Nanostructured lipid carriers; Fungal infections; Full factorial design; Design of experiment.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100524

Stability and sensory assessment of emulsions containing propolis extract and/or tocopheryl acetate
Provedor de dados: 60
Autores: Gonçalves,Gisele Mara Silva; Srebernich,Silvana Mariana; Souza,Jacqueline Alves de Macedo.
The prevention of skin aging has been one of the main aims of cosmetic products. Propolis and tocopheryl acetate can be promising substances because of their antioxidant properties. In this study, propolis extract was obtained and associated with tocopheryl acetate in a cream formulation, which then underwent stability and sensory assessment. The formulation containing propolis extract and tocopheryl acetate proved to be stable in the preliminary stability study, demonstrating gradual darkening and slight pH decrease when subjected to 60ºC for 28 days, but showing stability on rheological study. In the sensory analysis, the formulation containing these two components was preferred by the product testers over the base cream and creams containing propolis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant/use in cosmetic formulations; Cosmetics/sensory analysis; Propolis/extract; Propolis/use in cosmetics; Tocopheryl acetate/use in cosmetics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300016

Carboplatin: molecular mechanisms of action associated with chemoresistance
Provedor de dados: 60
Autores: Sousa,Graziele Fonseca de; Wlodarczyk,Samarina Rodrigues; Monteiro,Gisele.
Carboplatin is a derivative of cisplatin; it has a similar mechanism of action, but differs in terms of structure and toxicity. It was approved by the FDA in the 1980s and since then it has been widely used in the treatment of several tumor types. This agent is characterized by its ability to generate lesions in DNA through the formation of adducts with platinum, thereby inhibiting replication and transcription and leading to cell death. However, its use can lead to serious inconvenience arising from the development of resistance that some patients acquire during treatment, limiting the scope of its full potential. Currently, the biochemical mechanisms related to resistance are not precisely known. Therefore, knowledge of pathways associated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carboplatin/mechanisms of resistance; Cisplatin/mechanisms of resistance; Drugs/resistance; Molecular targets; Antitumors/platinum-based.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400693

Investigations of Acacia modesta Wall. leaves for in vitro anti-diabetic, proliferative and cytotoxic effects
Provedor de dados: 60
Autores: Saleem,Bushra; Islam,Muhammad; Saeed,Hamid; Imtiaz,Fariha; Asghar,Maryam; Saleem,Zikria; Mehmood,Azra; Naheed,Surriya.
ABSTRACT The leaves of Acacia modesta Wall. have been shown to possess diverse pharmacological properties. Therefore, we aimed at evaluating anti-diabetic, cytotoxic and proliferative effects of extracts of Acacia modesta Wall. leaves. After evaluating the primary and secondary metabolites, anti-diabetic activity of various extracts was assessed by α-amylase inhibition, glucose uptake by yeast cells and non-enzymatic glycosylation of hemoglobin assay. Cytotoxicity and proliferative potential was assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and short term proliferation assays, respectively, using human liver carcinoma cell line, HepG2. Among other extracts, chloroform extract exhibited 34.16% inhibition of α-amylase, 90.65%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acacia modesta Wall; /anti-diabetic effects/cytotoxic/in vitro; MTT; Phytochemical.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200609

Histology
Provedor de dados: 60
Autores: Curi,Rui.
Tipo: Info:eu-repo/semantics/report
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100031

Evaluation of the stability of Polymyxin B in saline and glucose solutions using LC-MS/MS
Provedor de dados: 60
Autores: Castilhos,Juliana Kich; Dillenburg,Thaís Luise; Antunes,Marina Venzon; Scribel,Letícia; Zavascki,Alexandre Prehn; Linden,Rafael; Verza,Simone Gasparin.
Polymyxins are a group of antibacterial substances and have remained the drugs of choice for treatment of resistant Gram-negative bacilli. Polymyxin B is administered by intravenous infusion and requires the reconstitution of lyophilized powder with 0.9% saline or 5% glucose solutions. To date, there is little information about polymyxin stability in different infusions solutions, especially at 40 ºC, a temperature that is recommended to study drug stability as it accelerates degradation reactions. Therefore, in this work an analytical method using LC-MS/MS was developed, validated and applied to determine the stability of polymyxin B diluted in 0.9% saline or 5% glucose solutions at 25 ºC and 40 ºC. The stability of polymyxin B solutions was evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymyxin; Stability; LC-MS/MS; Glucose solutions; Saline solutions.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100537

Efficacy and safety of cumaru syrup as complementary therapy in mild persistent asthma: a double-blind, randomized, placebo-controlled study
Provedor de dados: 60
Autores: Carvalho,Elisete Mendes; Cunha,Gilmara Holanda da; Fechine,Francisco Vagnaldo; Uchôa,Célia Regina Amaral; Moraes Filho,Manoel Odorico de; Bezerra,Fernando Antônio Frota; Moraes,Maria Elisabete Amaral de.
Amburana cearensis is a medicinal plant known as "cumaru". It is used in Northeast Brazil in the treatment of respiratory diseases. This was a randomized, double-blind, placebo-controlled study, with the aim of evaluating the efficacy and safety of cumaru syrup as complementary therapy in mild persistent asthma. The study consisted of 3 phases, pre-treatment, treatment and post-treatment. The primary efficacy outcome was comparison of the changes reported by patients of the cumaru and placebo groups after treatment, using the "Asthma Quality of Life Questionnaire" (AQLQ). The secondary outcome was the effect of cumaru syrup on lung function based on spirometry. The results showed that in the cumaru group, the proportion of patients who had global...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amburana cearensis/phytoterapy; Cumaru syrup/efficacy; Asthma/treatment.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400006

Isolation and characterization of lactic acid bacteria and yeasts from the Brazilian grape sourdough
Provedor de dados: 60
Autores: Aplevicz,Krischina Singer; Mazo,Jaciara Zarpellon; Ilha,Eunice Cassanego; Dinon,Andréia Zilio; Sant´Anna,Ernani Sebastião.
Sourdough is a mixture of flour and water fermented by lactic acid bacteria and yeast, with a large use in bakery products. This study was developed with Brazilian grape (Niagara rosada) sourdough obtained from spontaneous fermentation. The aim of this work was to characterize genotypic and phenotypically lactic acid bacteria and yeasts isolated from sourdough. The phenotypic identification for bacteria and yeasts was performed by using the kit API50CHL and 20CAUX and the genotypic characterization was performed by sequencing method. A total of four isolated strains were analyzed in this study. Two of these strains were phenotypically and genotypic identified as Lactobacillus paracasei and one as Saccharomyces cerevisiae. Another sample phenotypically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spontaneous fermentation; Sourdough; Brazilian grape/sourdough; Niagara rosada/sourdough; Lactic acid bacteria/phenotypic characterization; Lactic acid bacteria/genotypic characterization; Yeast/phenotypic characterization; Yeast/ genotypic characterization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200321

A simple HPLC method for the determination of halcinonide in lipid nanoparticles: development, validation, encapsulation efficiency, and  in vitro drug permeation
Provedor de dados: 60
Autores: Lopes,Clarissa Elize; Langoski,Gisele; Klein,Traudi; Ferrari,Priscileila Colerato; Farago,Paulo Vitor.
ABSTRACT Halcinonide is a high-potency topical glucocorticoid used for skin inflammation treatments that presents toxic systemic effects. A simple and quick analytical method to quantify the amount of halcinonide encapsulated into lipid nanoparticles, such as polymeric lipid-core nanoparticles and solid lipid nanoparticles, was developed and validated regarding the drug's encapsulation efficiency and in vitro permeation. The development and validation of the analytical method were carried out using the high performance liquid chromatography with the UV detection at 239 nm. The validation parameters were specificity, linearity, precision and accuracy, limits of detection and quantitation, and robustness. The method presented an isocratic flow rate of 1.0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Halcinonide/encapsulation efficiency; Halcinonide/quantification; Polymeric lipid-core nanoparticles; Solid lipid nanoparticles; Topical administration; Toxicity; High performance liquid chromatography/method validation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200604

Rational use of medicines: prescribing indicators at different levels of health care
Provedor de dados: 60
Autores: Ferreira,Maria Beatriz Cardoso; Heineck,Isabela; Flores,Liziane Maahs; Camargo,Aline Lins; Dal Pizzol,Tatiane da Silva; Torres,Iraci Lucena da Silva; Koenig,Alvaro; Trevisol,Daisson José; Melo,Adriana Mary Mestriner Felipe de; Cardoso,Teófilo Fernando Mazon; Monreal,Maria Tereza Ferreira Duenhas; Kadri,Mônica Cristina Toffoli.
This multicenter study aimed to investigate prescribing patterns of drugs at different levels of health care delivery in university-affiliated outpatient clinics located in eight cities in the South and Midwest of Brazil. All prescriptions collected were analyzed for various items, including WHO prescribing indicators. A total of 2,411 prescriptions were analyzed, and 469 drugs were identified. The number of drugs prescribed per encounter, the frequency of polypharmacy, and the percentage of encounters with at least one injection or antibiotic prescribed were higher in centers providing primary health care services, compared to those where this type of care is not provided. Most drugs (86.1%) were prescribed by generic name. In centers with primary health...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/rational use; Medicines/prescription; Health Care/quality indicators; Pharmacy/community services.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200015

Radioprotective profile of Urtica dioica L. seed extract on oxidative DNA-damage in liver tissue and whole blood of radiation-administered rats
Provedor de dados: 60
Autores: Çakır,Tahir; Yıldızhan,Kenan; Huyut,Zübeyir; Uyar,Ahmet; Arıhan,Okan.
It was aimed to investigate the radioprotective activity of Urtica dioica L. seed extract (UDSE) in the whole blood and liver of radiation-administered rats, both biochemically and immunohistochemically. 32 rats were divided into 4 groups (n:8). Control group (C): no administration for 10 days. Radiation group (IR): fed pellets for 10 days after exposure to radiation. Radiation + UDSE (IR+UDSE) group: exposed to radiation and fed UDSE for 10 days. UDSE group (UDSE): fed UDSE for 10 days. Radiation (5Gy) was given as a single fraction. 8-hydroxy-2-deoxyguanosine (8-OHdG) and deoxyguanosine (dG) levels were analyzed by biochemical method and glutathione peroxidase 1 (GPx-1) analyses were performed by immunohistochemical method in the liver and blood tissues...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioprotective; DNA damage; Antioxidant; Urtica.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100558

Pilot-project of implantation of pharmaceutical care close to the program of bipolar mood disorder of the Hospital of Clinics of Porto Alegre
Provedor de dados: 60
Autores: Ceresér,Keila Maria Mendes; Mascarenhas,Marcello Ávila; Santin,Aida; Kapczinski,Flávio.
The Bipolar Mood Disorder is characterized by the alternation of depressive crises with episodes of mania or euphoria, having these patients 15 to 35 times more chances of suicide, as compared with people without this disorder. The pharmacotherapy is fundamental for this disease, aiming to decrease the frequency of episodes and disease severity. In these patients, the polypharmacy has recently increased and one of the main difficulties is the adherence to treatment. The objective of this study was to contribute for the improvement of bipolar patients health conditions, developing their respective pharmacotherapeutic follow-up. Twenty eight adult bipolar patients who were participants of a specialized clinic within a tertiary hospital in Porto Alegre have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical Care; Bipolar Mood Disorder; Pharmacotherapy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200009

Programmed delivery of verapamil hydrochloride from tablet in a capsule device
Provedor de dados: 60
Autores: Sah,Mukesh Lal; Juyal,Vijay.
The aim of the present work was to develop a programmed drug delivery system which would be able to release the drug after 6 h of lag time by use of hydrophilic polymers. The capsule body was made impermeable by use of formaldehyde vapor treatment, while the cap was untreated. The capsule was filled with two layered tablets (tablet-in-capsule), followed by a sodium bicarbonate:citric acid mixture (SBCM) and lactose as bulking agent. Sodium alginate, chitosan, HPMC K15 and chitosan:sodium alginate complex (CSAC) were used as the rate modulating layer. Through combined use of HPMC K15 and adjusting the ratio of CSAC, the desired lag time of 6 h was obtained. The effect of the bulking agents on the lag time were also studied and it was found that the lag time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circadian rhythm; Chitosan/sodium alginate complex; HPMC K15; Lactose; Effervescent mixture.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200007

Analysis of spironolactone polymorphs in active pharmaceutical ingredients and their effect on tablet dissolution profiles
Provedor de dados: 60
Autores: Resende,Renata Cunha de; Viana,Olímpia Maria Martins Santos; Freitas,Jennifer Tavares Jacon; Bonfilio,Rudy; Ruela,André Luís Morais; Araújo,Magali Benjamim de.
ABSTRACT Spironolactone (SPR) is a steroidal drug administered as a potassium-sparing diuretic for high blood pressure treatment. The drug shows incomplete gastrointestinal absorption due to its poor aqueous solubility. The physicochemical properties of SPR in crystal forms I and II suggest that differences in their aqueous solubility may lead to a lack of bioequivalence between solid-state formulations. In this study, SPR polymorphs in five batches of active pharmaceutical ingredients (APIs) from three manufacturers were characterized using powder X-ray diffraction, infrared spectroscopy, thermal analysis, and solubility measurements. SPR tablets (50 mg) were manufactured in our laboratory using API in pure form II, and API in form II contaminated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spironolactone/dissolution profile; Spironolactone/pharmaceutical equivalence; Spironolactone/solubility; Spironolactone/polymorphism; Drugs/quality assessment.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400613

Critical evaluation of the off-label indication and of the risks associated to the use of multi-dose vials on the treatment of age-related macular degeneration
Provedor de dados: 60
Autores: Barbosa,Bruna Renata Dutra; Barbosa,Sávio Fujita; Tavares,Guilherme Diniz; Chacra,Nádia Araci Bou; Pinto,Terezinha de Jesus Andreoli.
Age-related macular degeneration (AMD) is an ocular inflammatory diseases treated mainly by means of a bevacizumab (Avastin®) or ranibizumab (Lucentis®) intravitreal injection. Among these drugs, only ranibizumab has a specific therapeutic indication for AMD. Considering that, the off-label use on ophthalmic therapy seems to become a rule when it should be an exception. Furthermore, bevacizumab presentation consists of multi-dose vials although it does not contain preservatives in its formula. The current literature review aimed at assessing the risks for the patient related to the use of off-label indication and multi-dose vials on AMD treatment. Considering this, the proposal related to the Brazilian Public Consultation no.10, dated September 12, 2012,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age-related macular degeneration/treatment; Ranibizumab/intravitreal injection; Bevacizumab/intravitreal injection; Ophthalmic therapy/off-label use; Off-label indication/patients risks; Multi-dose vials/patients risks.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100006

Much to celebrate!
Provedor de dados: 60
Tipo: Info:eu-repo/semantics/other
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100001

Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India
Provedor de dados: 60
Autores: Kumar,Sanjeet; Mahanti,Padma; Singh,Nihar Ranjan; Rath,Sakti Kant; Jena,Padan Kumar; Patra,Jayanta Kumar.
ABSTRACT Dioscorea pentaphylla L., a wild tuber is used both as food and medicines among different ethnic groups of Similipal Biosphere Reserve, India. Tubers are used against skin infections. In order to establish and confirm tribal claims, methanol extract was subjected to fractionation. The active fraction (DP1) was subsequently used for further purification and NMR (Nuclear magnetic resonance) characterization. The phytochemical analysis revealed the presence of saponin groups. The antibacterial activity of DP1 was done against selected bacterial strains (Salmonella typhi, Shigella flexneri, Streptococcus pyogenes, Streptococcus mutans and Vibrio cholerae) using DD (disc diffusion), AWD (agar well diffusion) and broth dilution assay. The activity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioscorea pentaphylla/extract/active fraction; Dioscorea pentaphylla/ antibacterial activity/antioxidant; NMR characterization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400623

Effects of Arctium lappa aqueous extract on lipid profile and hepatic enzyme levels of sucrose-induced metabolic syndrome in female rats
Provedor de dados: 60
Autores: Ahangarpour,Akram; Ali-Akbari,Fatemeh Ramazani; Mohaghegh,Seyyedeh Marjan; Asadinia,Ebtesam.
ABSTRACT Arctium lappa is known to have antioxidant and antidiabetic effects in traditional medicine. Objectives: The aim of this paper was to study the effects of A. lappa root extract (AE) on lipid profile and hepatic enzyme levels in sucrose-induced metabolic syndrome (MS) in female rats. The study used 40 adult female Wistar rats weighing 150 g-250 g randomly divided into five groups: control, metabolic syndrome (MS), metabolic syndrome+AE at 50,100, 200 mg/kg. MS was induced by administering 50% sucrose in drinking water for 6 weeks. AE was intra-peritoneally administered daily at doses of 50,100, and 200 mg/kg for two sequential weeks at the end of the fourth week in metabolic syndrome rats. Twenty-four hours after the last administration of AE,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lappa arctium/effects; Metabolic syndrome; Sucrose/lipid profile/hepatic enzymes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300425

Effect of peracetic acid on biofilms formed by Listeria monocytogenes strains isolated from a Brazilian cheese processing plant
Provedor de dados: 60
Autores: Lee,Sarah Hwa In; Barancelli,Giovana Verginia; Corassin,Carlos Humberto; Rosim,Roice Eliana; Coppa,Carolina Fernanda Sengling Cebin; Oliveira,Carlos Augusto Fernandes de.
ABSTRACT This study aimed to investigate the effect of peracetic acid (PAA, 0.5%) on adherent cells of three strains of Listeria monocytogenes strains belonging to serotypes 4b and 1/2b that had been previously isolated from the environment of a Brazilian cheese plant. The assays were conducted using polystyrene microplates and stainless steel coupons and the adhered cells were treated with PAA for 60, 120 and 180 s. On stainless steel, biofilms were partially inactivated by PAA after 60 s and almost 100% of the cells were damaged within 180 s using epifluorescence microscopy with LIVE/DEAD® staining. On polystyrene microplates, PAA decreased (P&lt;0.05) biofilm biomass produced by the three L. monocytogenes isolates at 60 s, when compared with controls...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peracetic acid (PAA)/ biofilm/effects; L. monocytogenes; Dairy plants/Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300610

Formulation and evaluation of mucoadhesive buccal patch of acyclovir utilizing inclusion phenomenon
Provedor de dados: 60
Autores: Saxena,Ankita; Tewari,Gulab; Saraf,Shubhini Awasthi.
Mucoadhesive buccal patch releasing drug in the oral cavity at a predetermined rate may present distinct advantages over traditional dosage forms, such as tablets, gels and solutions. A buccal patch for systemic administration of acyclovir in the oral cavity was developed using polymers hydroxy propyl methyl cellulose (K4M), hydroxy propyl methyl cellulose (K15M), sodium carboxy methyl cellulose and poly vinyl pyrolidone (K30), plasticizer poly ethylene glycol (400) and a backing membrane of Eudragit (RL100). The films were evaluated in terms of swelling, residence time, mucoadhesion, release, and organoleptic properties. The optimized films showed lower release as compared to controlled drug delivery systems. Hence, an inclusion complex of acyclovir was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buccal patches/drug release; Inclusion complexes/use; Acyclovir; Mucoadhesion.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400026

Active ingredients, mechanisms of action and efficacy tests of antipollution cosmetic and personal care products
Provedor de dados: 60
Autores: Velasco,Maria Valéria Robles; Sauce,Rafael; Oliveira,Camila Areias de; Pinto,Claudinéia A.S. de Oliveira; Martinez,Renata Miliani; Baah,Stephanie; Almeida,Tânia Santos; Rosado,Catarina; Baby,André Rolim.
Urban population around the globe is direct exposed to the pollution caused by several sources (vehicles, industries, smokes etc.) and primary pollutants are divided in particulate matter and toxic gases. Current researches in populous countries indicated that exposure to pollution could affect sebum composition, stratum corneum quality and signs of skin aging. Hair and scalp are also affected by the excessive exposure to pollutants, resulting in a dull, dry and lifeless appearance. Cosmetics have been evolved conceptual and scientifically to achieve substantial effectiveness against pollution damaging on the cutaneous tissue, involving the development of innovative multipurpose active ingredients and efficacy tests, skilled to prove the protection and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Active ingredients; Antipollution; Cosmetics; Efficacy tests.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700402

Reverse phase high-performance liquid chromatography for quantification of hydroxymethylnitrofurazone in polymeric nanoparticles
Provedor de dados: 60
Autores: Monteiro,Lis Marie; Tavares,Guilherme Diniz; Ferreira,Elizabeth Igne; Consiglieri,Vladi Olga; Bou-Chacra,Nadia Araci; Löbenberg,Raimar.
Hydroxymethylnitrofurazone (NFOH) is a new compound with potential leishmanicidal and trypanocidal activity. Despite its effectiveness, the formulators have to overcome its poor aqueous solubility. Recently, polymeric nano-scale drug delivery systems have proposed for the treatment of neglected diseases. As several studies have confirmed the advantages of such formulations, and this approach provides new analytical challenges, including the need to detect trace amounts of the drug. A suitable method was developed and validated for NFOH determination bound to poly (n-butylcyanoacrylate) (PBCA) nanoparticles. The chromatographic separation was achieved using a C18 column maintained at 25 ºC and an isocratic mobile phase consisting of water and acetonitrile:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxymethylnitrofurazone/determinação; Nanoparticle; High-performance liquid chromatography/reverse phase/quantitative analysis; Poly(n-butylcyanoacrylate)/nanoparticles/encapsulation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300561

Dendritic surface functionalization of nanomaterials: controlling properties and functions for biomedical applications
Provedor de dados: 60
Autores: Nazemi,Ali; Gillies,Elizabeth R.
A wide variety of nanomaterials have demonstrated promise in medical applications such as drug delivery and imaging. In these applications, the surface chemistry of the materials is critical as it plays an important role in determining the toxicity and biodistribution behavior of the material. We review here the functionalization of nanomaterials with dendrons as an efficient method to alter the surface chemistry of the materials, introducing new properties and functions. Described here is the functionalization of superparamagnetic iron oxide nanoparticles (SPIO) with dendritic guanidines to enhance their transport into cells for magnetic resonance imaging applications. The introduction of dendrons bearing peripheral hydroxyls, amines, guanidines,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimer; Superparamagnetic Iron Oxide Nanoparticles; Polymersome; Pharmaceutical applications.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700003

Possible pharmacological interactions in hypertensive and/or diabetic elderly in family health units at Blumenau (SC)
Provedor de dados: 60
Autores: Codagnone Neto,Vicente; Garcia,Victor Pundek; Santa Helena,Ernani Tiaraju de.
The aim of this study was to examine the possible adverse drug-drug interactions in the elderly population (&gt;60 years) diagnosed either with diabetes, arterial hypertension or both, at a Family Health Unit (FHU) clinic in Blumenau, state of Santa Catarina, Brazil. For this purpose 318 subjects were interviewed using a pretested form with social and demographic aspects regarding their disease and its complications. All drugs used by this population were grouped, and the drug-drug interactions were detected by using the "Drug Interaction Checker" software, and classified for frequency and severity. The average age of patients was 70.6 years, with a higher number of female patients (216; 67.9%). Most subjects were being treated for both diseases (149;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug-drug interaction; Systemic arterial hypertension/use of medications; Diabetes Mellitus/use of medications; Elderly/use of medications; Family health program.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400022

Evaluating the effects of vanadyl sulfate on biomarkers of oxidative stress and inflammation in renal tissue of rats with diabetes type 2
Provedor de dados: 60
Autores: Fereshteh,Bahmani; Ali-Reza,Afsharipour; Nastaran,Manteghie; Mohsen,Taghizadeh; Mehdi,Mirhashemi Seyyed.
Vanadyl sulfate (VS) is an ingredient in some food supplements and experimental drugs. This study was designed to assay the effects of VS on biomarkers of oxidative stress and inflammation in renal tissue of rats with diabetes type 2. 30 male Wistar rats were divided into three equal groups as follow: non-diabetics, non-treated diabetics and VS-treated diabetics. Diabetes type 2 has been induced through high fat diet and fructose in the animals. Diabetic rats were treated with 25 mg/kgBW of VS in water for 12 weeks. At the end of study, glucose and insulin were measured using commercially available kits in serum and biomarkers of oxidative stress and inflammation in renal homogenates of animals were measured by related methods. Compared to controls,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vanadyl Sulfate; IL-1; IL-10 Oxidative Stress Inflammations.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100576

Assessing the overall medication use by elderly people in a Brazilian hospital using the start/stopp criteria version 2
Provedor de dados: 60
Autores: Pereira,Thayná Ferreira Furtado; Soares,Alessandra de Sá; Trevisol,Daisson José; Schuelter-Trevisol,Fabiana.
To estimate the frequency of the use of medicines listed in the Screening Tool to Alert Doctors to the Right Treatment (START) and Screening Tool of Older Person’s Prescriptions (STOPP) criteria version 2 among the elderly. A cross-sectional study was conducted on elderly who were attended in medical clinic and cardiology sectors in a hospital in southern Brazil attended at a hospital from February through September 2016. A data-collection tool was used to obtain information on variables, such as demographic and clinical data, and medications used before and during the hospitalization period. The adequacy of the medicines taken was examined with regard to omission (START) or inappropriate use (STOPP). This study was approved by the Research Ethics...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug utilization; Potentially inappropriate Medication list; Aged; Polypharmacy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100531

Poly-ε-caprolactone microspheres containing interferon alpha as alternative formulations for the treatment of chronic hepatitis C
Provedor de dados: 60
Autores: Melo,Cristiane da Silva; Pereira,Bruno Gonçalves; Silva-Cunha,Armando; Fialho,Sílvia Ligório.
Interferon-alpha (IFN-alpha) is one of the main drugs used in the treatment of hepatitis C. Use of IFN-alpha has some limitations that result in poor treatment efficacy and low patient compliance. Therefore, the aim of this study was to develop poly-ε-caprolactone (PCL) microspheres containing IFN-alpha as an alternative for the treatment of chronic hepatitis C. Microspheres were prepared using the multiple emulsion followed by solvent evaporation technique. Particle size, surface morphology, drug content and encapsulation efficiency of the microspheres produced were evaluated. The stability of the formulation was assessed after 90 days at -20ºC. An in vitro release study was performed in PBS. In vitro cytotoxicity of the formulation was studied using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon alfa; Hepatitis C/treatment; Poly- ε-caprolactone/microspheres; Poly- ε-caprolactone/development.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100006

Formulation and in vitro evaluation of mucoadhesive controlled release matrix tablets of flurbiprofen using response surface methodology
Provedor de dados: 60
Autores: Khalid,Ikrima; Ahmad,Mahmood; Minhas,Muhammad Usman; Sohail,Muhammad.
The objective of the current study was to formulate mucoadhesive controlled release matrix tablets of flurbiprofen and to optimize its drug release profile and bioadhesion using response surface methodology. Tablets were prepared via a direct compression technique and evaluated for in vitro dissolution parameters and bioadhesive strength. A central composite design for two factors at five levels each was employed for the study. Carbopol 934 and sodium carboxymethylcellulose were taken as independent variables. Fourier transform infrared (FTIR) spectroscopy studies were performed to observe the stability of the drug during direct compression and to check for a drug-polymer interaction. Various kinetic models were applied to evaluate drug release from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flurbifrofen/mucoadhesive controlled release tablets/formulation; Flurbifrofen/mucoadhesive controlled release tablets/in vitroevaluation; Drugs/release profile; Drugs/bioadhesion; Tablets/direct compression.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300493

Vasodilator activity of extracts of field Alpinia purpurata (Vieill) K: Schum and A. zerumbet (Pers.) Burtt et Smith cultured in vitro
Provedor de dados: 60
Autores: Victório,Cristiane Pimentel; Kuster,Ricardo Machado; Moura,Roberto Soares de; Lage,Celso Luiz Salgueiro.
Nowadays, the high blood pressure is one of the main causes of death and cardiovascular diseases. Vasodilator drugs are frequently used to treat arterial hypertension. Experiments were undertaken to determine whether hydroalcoholic extracts obtained from leaves of field-grown Alpinia purpurata and A. zerumbet cultured in vitro under different plant growth regulators induce a vasodilator effect on Wistar rat mesenteric vascular bed pre-contracted with norepinephrine. Plant extracts were able to induce a long-lasting endothelium-dependent vasodilation. Efficiency on activity of A. purpurata reached 87% at concentration of 60 μg. The extract of A. zerumbet maintained in medium containing IAA, induced the relaxation (17.4%) at 90 μg, as compared to the control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpinia purpurata (Vieill) K. Schum./pharmacognosy; A. zerumbet (Pers.) Burtt et Smith./pharmacognosy; Natural vasodilators/experimental studies; Plant extracts/vasodilator action; Plant tissue/culture; Endothelium-dependent; Flavonoids; Medicinal Plant; Plant growth/regulators; Zingiberaceae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300017

2-Phenitidine derivatives as suitable inhibitors of butyrylcholinesterase
Provedor de dados: 60
Autores: Abbasi,Muhammad Athar; Aziz-ur-Rehman,; Qureshi,Muhammad Zahid; Khan,Farhan Mehmood; Khan,Khalid Mohmmed; Ashraf,Muhammad; Afzal,Iftikhar.
This manuscript reports the synthesis of a series of N-substituted derivatives of 2-phenitidine. First, the reaction of 2-phenitidine (1) with benzene sulfonyl chloride (2) yielded N-(2-ethoxyphenyl) benzenesulfonamide (3), which further on treatment with sodium hydride and alkyl halides (4a-g) furnished into new sulfonamides (5a-g). Second, the phenitidine reacted with benzoyl chloride (6) and acetyl chloride (8) to yield the reported N-benzoyl phenitidine (7) and N-acetyl phenitidine (9), respectively. These derivatives were characterized by infrared spectroscopy, ¹H-NMR, and EI-MS, and then screened against acetylcholinesterase, butylcholinesterase, and lipoxygenase enzyme, and were found to be potent inhibitors of butyrylcholinesterase alone.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-Phenitidine/inhibitor activity; Sulfonamides; Acetamide; Benzamide; Butyrylcholinesterase.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100014

Comparative antioxidant and bioavailability studies of Vitamin C in Phyllanthus emblica Linn. and its combinations with Piper nigrum Linn. and Zingiber officinale Roscoe
Provedor de dados: 60
Autores: Somasekhar,Vanita; Ashok,Purnima; Kameswari,Sri Adibatla Renuka; Rajendran,Ramaswamy; Singh,Rajpreet.
ABSTRACT Phyllanthus emblica Linn. (amla) is used in Ayurveda, the ancient Indian system of medicine and its major constituent is vitamin C which has effective free radical scavenging property. The purpose of this study was to evaluate the in vitro antioxidant activity and the bioavailability profile of vitamin C in amla and its combinations with piperine and ginger in comparison to synthetic vitamin C using New Zealand rabbits. In vitro antioxidant activity studies of synthetic vitamin C, amla, amla with piperine and amla with ginger were carried out using different models such as 2,2-Diphenyl-1-picrylhydrazyl, Nitric Oxide, Hydrogen peroxide scavenging methods, Total reductive capability and Oxygen Radical Absorbance Capacity estimation. The study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phyllanthus emblica Linn; Vitamin C/antioxidant activity/in vitro study; Vitamin C/bioavailability; Vitamin C/synthetic/antioxidant activity; Ginger; Piperine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100005

Educational level, socio-economic status and relationship with quality of life in elderly residents of the city of Porto Alegre/RS, Brazil
Provedor de dados: 60
Autores: Colet,Christiane de Fátima; Mayorga,Paulo; Amador,Tânia Alves.
The objective of this study was to verify the index of quality of life of elderly individuals belonging to groups, from different socioeconomic strata in the city of Porto Alegre, Rio Grande do Sul State. The research adopted a transversal model, used to collect data from the SF-36 questionnaire. The sampling is the intentional type and comprised: 61 elderly people in Class A, 80 in Class C, and 84 in Class E. The research was approved by the Committee of Ethics in Research/UFRGS. In relation to the quality of life, Class A presented higher scores than did Classes C and E on the pain, vitality, social aspect and mental health areas. The results also showed a significant difference in quality of life among university educated individuals versus the other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly/quality of life; Elderly/health.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400023

Adverse reactions to docetaxel: an active survey
Provedor de dados: 60
Autores: Pereira,Leandro Cabral; Nogueira,Thaísa Amorim; Barbosa,Leandro Augusto de Oliveira; Calil-Elias,Sabrina; Castilho,Selma Rodrigues de.
The rates of breast cancer mortality remain high in Brazil. Docetaxel is a semi-synthetic taxane used to treat various tumors, particularly tumors of the breast, lung and prostate. In this study ADR that occurred in 45 docetaxel users with breast cancer were surveyed. They were identified by type, causality (Naranjo algorithm and World Health Organization categories) and, if considered probable or defined, rated for severity according to SOBRAFO proposal (2007). A total of 325 ADR were observed: 165 in the first, 137 in the second and 23 in the third cycle. Fifty seven ADR were immediate and the others, late. Fatigue and exhaustion for more than five days, classified as Grade 3 by SOBRAFO (2007), were reported as the primary late RAM. There was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Docetaxel/adverse reactions; Drugs/adverse reactions; Breast Cancer/tretament/reactions.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300551

In vitro evaluation of sustained released matrix tablets containing ibuprofen: a model poorly water-soluble drug
Provedor de dados: 60
Autores: Guerra-Ponce,Wendy Leticia; Gracia-Vásquez,Sandra Leticia; González-Barranco,Patricia; Camacho-Mora,Ivonne Antonieta; Gracia-Vásquez,Yolanda Araceli; Orozco-Beltrán,Elizabeth; Felton,Linda Anne.
ABSTRACT A matrix system was developed that releases ibuprofen (IB) over a 12-hour period and the influence of the polymer type and concentration on the release rate of the drug was evaluated. Tablets containing different concentrations of Carbopol (CP), hydroxypropyl methylcellulose (HPMC), or ethyl cellulose (EC) were prepared using direct compression and the drug content, content uniformity, hardness, friability, dissolution performance, and in vitro release kinetics were examined. Formulated tablets were found to be within acceptable limits for physical and chemical parameters. The release kinetics of the Carbopol(r)971P 8% formulation showed the best linearity (r 2 =0.977) in fitting zero-order kinetics, suggesting the release rate was time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ibuprofen/study; Ibuprofen/sustained release tablets; Ibuprofen/formulation/carbopol; Ibuprofen/formulation/hydroxypropyl methylcellulose; Ibuprofen/formulations/ethyl cellulose; Ibuprofen/formulations/release kinetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400751

Effect of alcoholic beverages on progeny and reproduction of mice
Provedor de dados: 60
Autores: Figueiro,Fernanda Dias; Bispo,Ana Clara Cerato; Guarido,Karla Lorena; Marianno,Priscila; Costa,Gabriel de Araújo; Morimoto,Helena Kaminami; Salles,Maria José Sparça.
ABSTRACT Alcohol is the most commonly consumed substance in the world. The objective of this study was to evaluate the influence of alcoholic beverages on male reproduction and possible alterations in their offspring. The mice were divided into 4 groups: beer, wine, cachaça (a type of sugarcane rum), with ethanol concentrations of 1.9 g/kg, and control group treated with PBS. The treatment period was 35 days. The animals which received cachaça, demonstrated significant weight loss in the testes and epididymis. The alcoholic beverages promoted significant testosterone level and fertilization index diminution, and morphological alterations in the spermatozoa. The beer group presented decreased implantation sites and a high frequency of dominant lethal. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholic beverages/analysis; Wine/adverse effects; Beer/adverse effects; Cachaça/adverse effects; Reproduction/drugs effects.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400612

Topical antiinflammatory activity and chemical composition of the epicuticular wax from the leaves of Eugenia beaurepaireana (Myrtaceae)
Provedor de dados: 60
Autores: Magina,Michele Debiasi Alberton; Pietrovski,Evelise Fernades; Gomig,Franciane; Falkenberg,Daniel de Barcellos; Cabrini,Daniela Almeida; Otuki,Michel Fleith; Pizzollati,Moacir Geraldo; Brighente,Inês Maria Costa.
In order to verify the topical antiinflammatory effect of epicuticular wax from leaves of Eugenia beaurepaireana, it was tested in mice croton oil-induced inflammation. Our findings show that topical application of Eugenia beaurepaireana epicuticular wax was significantly active in inhibiting both oedema (Inhibitory dose 50 % (ID50) = 0.31 (0.26 - 0.39) mg.ear -1, inhibition = 79 ± 6 %) and tissue myeloperoxidase activity (indicative of polymorphonuclear leukocytes influx) (ID50 =0.34 (0.20 - 0.41) mg.ear -1, inhibition = 77 ± 4 %) in mice ear treated with croton oil. Two main compounds were detected on epicuticular wax of E. beaurepaireana. These compounds were identified as α- and β-amyrin by flame ionization detection (GC-FID) and spectroscopic methods...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenia beaurepaireana/pharmacognosy; Α-amyrin; Β-amyrin; Epicuticular wax/antiinflammatory activity; Ear oedema/experimental study.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100021

Reduced bioavailability of cyclosporine A in rats by mung bean seed coat extract
Provedor de dados: 60
Autores: Li,Xiping; Gao,Ping; Zhang,Chengliang; Wu,Tao; Xu,Yanjiao; Liu,Dong.
Mung bean seed coat (MBSC) is a healthcare product in Asian countries. The aim of this study was to investigate the effect of an MBSC ethanol extract on the bioavailability of cyclosporine A (CsA) in rats. Rats were orally dosed with CsA alone or in combination with MBSC ethanol extracts (500 mg/kg, p.o.). The blood levels of CsA were assayed by liquid chromatography with an electrospray ionization source and tandem mass spectrometry (LC-MS/MS). The everted rat intestinal sac technique was used to determine the influence of MBSC on the absorption of CsA. The results reveal that combined CsA intake with MBSC decreased the Cmax, AUC0-t, t1/2z and MRT0-t values of CsA by 24.96%, 47.28%, 34.73% and 23.58%, respectively (P&lt;0.05), and significantly raised the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mung bean/seed coat/health care use; Mung bean/seed coat/ethanolic extract/properties; Cyclosporine A/bioavailability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300591

Influence of monoolein on progesterone transdermal delivery
Provedor de dados: 60
Autores: Quintão,Wanessa de Souza Cardoso; Matos,Breno Noronha; Reis,Thaiene Avila; Barreto,Lívia Cristina de Sá; Gratieri,Taís; Gelfuso,Guilherme Msrtins.
abstract This work aimed to investigate in vitro the influence of monoolein (MO) on progesterone (PG) transdermal delivery and skin retention. Information about the role of MO as an absorption enhancer for lipophilic molecules can help on innovative product development capable of delivering the hormone through the skin in a consistent manner, improving transdermal therapy of hormonal replacement. MO was dispersed in propylene glycol under heat at concentrations of 0% (control), 5% w/w, 10% w/w and 20% w/w. Then, 0.6% of PG (w/w) was added to each formulation. Permeation profile of the hormone was determined in vitro for 48 h using porcine skin in Franz diffusion cells. PG permeation doubled when 5% (w/w) of MO was present in formulation in comparison to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monoolein/influence/transdermal therapy; Progesterone/transdermal delivery; Progesterone/skin retention; Hormonal replacement/transdermal therapy; Skin permeation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400923

Multifunctional photoprotection: corrective makeup for cutaneous dyschromias
Provedor de dados: 60
Autores: Gonçalves,Felipe da Silva; Giovanini,Iris Ramos Torres; Cerqueira,Cristal; Ricci Junior,Eduardo; Freitas,Zaida Maria Faria de; Santos,Elisabete Pereira dos.
The aim of this study was to develop and evaluate a corrective and photoprotective makeup for patients with dyschromias. An emulsion was prepared and pigment mixtures were incorporated in the formulation, producing five shades of corrective makeup: BEIGE (I, II, III), BRONZE and TAN. The sun protection factor (SPF) and UVA/UVB ratio of the corrective makeup were determined using spectrophotometry with a Labsphere® analyser. The spreadability, occlusivity, stability, and photostability of the photoprotective formulations were also evaluated. For all formulations there was no statistical difference among them (p &gt; 0.05) in terms of spreadability, occlusivity and SPF. They were considered to be photostable under solar radiation, with variations in SPF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corrective makeup; Dyschromias; Sunscreens; Emulsions cosmetics.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400613

Inorganic UV filters
Provedor de dados: 60
Autores: Manaia,Eloísa Berbel; Kaminski,Renata Cristina Kiatkoski; Corrêa,Marcos Antonio; Chiavacci,Leila Aparecida.
Nowadays, concern over skin cancer has been growing more and more, especially in tropical countries where the incidence of UVA/B radiation is higher. The correct use of sunscreen is the most efficient way to prevent the development of this disease. The ingredients of sunscreen can be organic and/or inorganic sun filters. Inorganic filters present some advantages over organic filters, such as photostability, non-irritability and broad spectrum protection. Nevertheless, inorganic filters have a whitening effect in sunscreen formulations owing to the high refractive index, decreasing their esthetic appeal. Many techniques have been developed to overcome this problem and among them, the use of nanotechnology stands out. The estimated amount of nanomaterial in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skin cancer; Sunscreen; Inorganic UV filter.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200002

Fluorometric quantification of protoporphyrin IX in biological skin samples from in vitro penetration/permeation studies
Provedor de dados: 60
Autores: Rossetti,Fábia Cristina; Depieri,Lívia Vieira; Tedesco,Antônio Cláudio; Bentley,Maria Vitória Lopes Badra.
A fluorometric analytical method was developed for quantification of protoporphyrin IX (PpIX) in skin samples and receptor phase solution after in vitro cutaneous penetration/permeation studies. Analytical conditions used were: excitation and emission wavelengths: 400 nm and 632 nm; bandwidth: 0.5 nm; excitation and emission slits: 10/10. PpIX was recovered from two different layers of skin, the stratum corneum (SC) and the epidermis plus dermis ([E+D]), by vortex homogenization, probe and bath sonication, using DMSO as an extraction solvent. The detection and quantification limits were 0.002 and 0.005 μg/mL, respectively. The assay was linear from 0.005 - 0.5 μg/mL. The within-day and between-day assay precision and accuracy in DMSO and receptor phase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluorometric quantification; Protoporphyrin IX/determination; Photodynamic therapy; Porcine skin; Cutaneous penetration/in vitro study; Cutaneous permeation/in vitro study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400017

Development of a new analytical method for determination of acetylsalicylic and salicylic acids in tablets by reversed phase liquid chromatography
Provedor de dados: 60
Autores: Aguiar,José Luiz Neves de; Leandro,Katia Christina; Abrantes,Shirley de Mello Pereira; Albert,André Luis Mazzei.
Acetylsalicylic acid (AAS) is a drug utilized as analgesic, anti-inflammatory, and antipyretic medication, available worldwide and commonly used in Brazil. Salicylic acid (AS) is a precursor in AAS synthesis and is also produced during its degradation. The official United States Pharmacopoeia (USP) suggests the determination of these drugs by high performance liquid chromatography (HPLC), with ultraviolet detection, but this method has neither a high sensitivity (S AAS=0.12 mAbs/(μg/mL) and S AS=0.48 mAbs/(μg/mL)) nor resolution (Rs=1.61). The purpose of this study was to develop a new more adequate, accurate method by liquid phase chromatography than the current official methodology, and to use this new method in the determination of the tenors of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography; Acetylsalicylic acid/determination in tablets; Salicylic acid/determination in tablets; Tablets/quantitative analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400016

Obligations, precautions and pending issues in regulatory development for radiopharmaceuticals in Brazil
Provedor de dados: 60
Autores: Gamboa,Maryelle Moreira Lima; Roesch,Heveline Rayane Moura; Lemos,Vanessa Pinheiro Amaral; Rocha,Bruna Oliveira; Santos-Oliveira,Ralph.
Radiopharmaceuticals are compounds that have a radionuclide and may be gamma-radiation emitter (γ) or positrons emitter (β+), linked to a molecule with specific diagnostic and therapeutic purposes. The progress in the use of radiopharmaceuticals has culminated to a sector in common with other types of drugs: regulation and surveillance. From 2006 on, production, marketing and use of these drugs were open to the Brazilian market granting much more freedom due to the Constitutional Amendment 49, resulting from the previous Constitutional Amendment 199/03 which removes the Union monopoly for this kind of manipulation and granted this production to other nuclear medicine. From this date on, the amount of this type of sold product have been greatly increased,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals/regulation; Radiopharmaceuticals/ surveillance; Radiopharmaceuticals/pharmacovigilance; Pharmacovigilance/practice in radiopharmaceuticals drugs; Brazilian legislation/radiopharmaceuticals control.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200285

Developing methods to compare tablet formulations of atorvastatin
Provedor de dados: 60
Autores: Oliveira,Marcelo Antonio de; Lacerda,Caroline Dutra; Bonella,André Fazôlo.
Atorvastatin (ATV) is an antilipemic drug of great interest to the pharmaceutical industry. ATV does not appear in the monographs of Brazilian pharmacopoeia, and analytical methodologies for its determination have been validated. The chromatographic conditions used included: RP-18 column-octadecylsilane (250 x 4.6 mm, 5 mm), detection at 238 nm, mobile phase containing 0.1% phosphoric acid and acetonitrile (35:65% v/v), flow at 1.5 mL min-1, oven temperature at 30ºC, and injection volume of 10 mL. ATV is classified as a class II product, according to the biopharmaceutical classification system. As such, a dissolution test was proposed to evaluate pharmaceutical formulations on the market today, under the following conditions: water as a dissolution medium,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical formulations/evaluation; Atorvastatin; Drugs/dissolution test; Drugs/qualitative analysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400024

Physical characterization of multiparticulate systems
Provedor de dados: 60
Autores: Issa,Michele Georges; Souza,Natalia Vieira de; Duque,Marcelo Dutra; Ferraz,Humberto Gomes.
ABSTRACT The search for new pharmaceutical dosage forms and different drug delivery systems already used in therapeutics is a global trend, serving as an opportunity to expand the portfolio for the pharmaceutical industry. In this context, multiparticulate systems, such as pellets, granules, and minitablets, represent an attractive alternative, given the range of possibilities they provide. Among the methods used in the production of these systems, we highlight the process of extrusion-spheronization for pellet manufacture, wet granulation and hot-melt extrusion for the obtention of granules, and direct compression for minitablets. Although highly versatile, depending on the technology chosen, many processes and formulation variables can influence the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiparticulate systems/physical characterization; Pellets; Granules; Minitablets.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400403

Immediate and long-term effects of polysaccharides-based formulations on human skin
Provedor de dados: 60
Autores: Camargo Junior,Flavio Bueno de; Gaspar,Lorena Rigo; Campos,Patrícia Maria Berardo Gonçalves Maia.
A new trend in cosmetic formulations is the use of biotechnological raw materials as the polysaccharides from Klebsiella pneumoniae, which are supposed to enhance cell renewal, improve skin hydration and micro-relief. Botanical extracts of Myrtus communis leaves contain different sugars, which may provide the same benefits. Thus, the objective of this study was to evaluate through objective and subjective analysis the immediate and long-term effects of cosmetic formulations containing polysaccharides biotechnologically-originated and / or the ones contained in Myrtus communis extracts. Three polysaccharide-based and placebo formulations were applied on the forearm skin of 40 volunteers. Skin hydration, transepidermal water loss (TEWL), viscoelasticity and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polysaccharides/cosmetic use; Klebsiella pneumoniae/cosmetic use; Myrtus communis/cosmetic use; Cosmetic formulations/evaluation; Human skin/hydration; Cosmetology/use of biotechnology; Biotecnological raw materials/use in cosmetology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300022

Polymorphism: an evaluation of the potential risk to the quality of drug products from the Farmácia Popular Rede Própria
Provedor de dados: 60
Autores: Santos,Olímpia Maria Martins; Reis,Maria Esther Dias; Jacon,Jennifer Tavares; Lino,Mônica Esselin de Sousa; Simões,Juliana Savioli; Doriguetto,Antonio Carlos.
Polymorphism in solids is a common phenomenon in drugs, which can lead to compromised quality due to changes in their physicochemical properties, particularly solubility, and, therefore, reduce bioavailability. Herein, a bibliographic survey was performed based on key issues and studies related to polymorphism in active pharmaceutical ingredient (APIs) present in medications from the Farmácia Popular Rede Própria. Polymorphism must be controlled to prevent possible ineffective therapy and/or improper dosage. Few mandatory tests for the identification and control of polymorphism in medications are currently available, which can result in serious public health concerns.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Medicines/quality control; Medicines/solubility; Medicines/bioavailability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100002

Development and validation of a stability indicating high performance liquid chromatography method for trimethobenzamide
Provedor de dados: 60
Autores: Swamy,Narayana; Reddy,Venkata Krishna; Thorat,Poonam; Sengupta,Pinaki.
A simple, accurate, precise and robust stability indicating RP-HPLC assay method has been developed for the estimation of trimethobenzamide in stress sample. An isocratic separation of trimethobenzamide was achieved on Kromasil 100 C-18 column (250 X 4.6mm, 5µ) with a flow rate of 1.0 ml/min and by using a photodiode array detector to detect the analyte at 213nm. The optimized mobile phase consisted of methanol: ammonium formate (44:56, v/v). The drug was subjected to different forced degradation conditions according to ICH guidelines including acid, base, neutral hydrolysis, oxidation, photolysis and thermal degradation. Degradation products were found only in basic and oxidative degradation conditions. All the degradation products got eluted in an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trimethobenzamide; Stability indicating assay method; Method development and validation; RP-HPLC.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100582

Agrobacterium rhizogenes-mediated transformation of Hypericum sinaicum L. for the development of hairy roots containing hypericin
Provedor de dados: 60
Autores: Khlifa,Heba Desouky; Klimek-Chodacka,Magdalena; Baranski,Rafal; Combik,Michal; Taha,Hussein Sayed.
Hypericum sinaicum L. is an endangered Egyptian medicinal plant of high importance due to the presence of naphthodianthrones (hypericins), which have photodynamic properties and pharmaceutical potential. We sought to assess H. sinaicum ability to develop hairy roots that could be cultured in contained conditions in vitro and used as a source for hypericin production. We used four A. rhizogenes strains differing in their plasmids and chromosomal backgrounds to inoculate excised H. sinaicum root, stem and leaf explants to induce hairy root development. Additionally, inoculum was applied to shoots held in Rockwool cubes supporting their stand after removal of the root system. All explant types were susceptible to A. rhizogenes although stem explants responded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Medicinal plant; Naphthodianthrones; Plant genetic engineering.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100590

Synergistic effect of ibuprofen with itraconazole and fluconazole against Cryptococcus neoformans
Provedor de dados: 60
Autores: Rocha,Letícia Fernandes da; Pippi,Bruna; Fuentefria,Alexandre Meneghello; Mezzari,Adelina.
The present study investigated the association of the non-steroidal anti-inflammatory drug ibuprofen with itraconazole, fluconazole and amphotericin B against Cryptococcus neoformans isolates. The minimal inhibitory concentration (MIC) was found according to M27-A3 protocol and in vitro interactions were evaluated using checkerboard microdilution method. Synergism was demonstrated between azoles and ibuprofen for most isolates. However, no synergistic effects were seen when amphotericin B was combined with ibuprofen. Therefore, our results suggest that ibuprofen presents clinical potential when combined with azole drugs in the treatment of cryptococcosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcus neoformans; Ibuprofen; Itraconazole; Fluconazole; Synergism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100577

HPLC method development and validation for the estimation of Axitinib in rabbit plasma
Provedor de dados: 60
Autores: Suneetha,Achanta; Donepudi,Sharmila.
ABSTRACT A rapid, sensitive, and accurate high performance liquid chromatography for the determination of Axitinib (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinib is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which works by slowing or stopping the growth of cancer cells. The chromatographic separation was performed on a Waters 2695, Kromosil (150 mm × 4.6 mm, 5 µm) column using a mobile phase containing buffer (pH 4.6) and acetonitrile in the ratio of 65:35 v/v with a flow rate of1 mL/min. The analyte and internal standard were extracted using liquid-liquid extraction with acetonitrile. The elution was detected by photo diode array detector at 320 nm.The total chromatographic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography/method validation; Axitinib/determination; Axitinib/ kidney cancer treatment; Crizotinibe; Rabbit plasma; Tyrosine kinase inhibitor.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300607

The evaluation of coated granules to mask the bitter taste of dihydroartemisinin
Provedor de dados: 60
Autores: Shahzad,Yasser; Shah,Syed Nisar Hussain; Atique,Shabbar; Ansari,Muhammad Tayyab; Bashir,Farooq; Hussain,Talib.
The purpose of this study was to mask the bitter taste imparted by dihydroartemisinin (DHA) by the use of different coating materials. Trial-1 and trial-2 were conducted to prepare the DHA granules. The granules produced from trial-1 were irregular in shape and smaller in size while the trial-2 granules were more regular and larger in size. The granules obtained from both trials were then coated with two different coating methods, namely A and B, depending upon coating material. The trial-2 granules showed better flow properties than trial-1 granules. In vitro dissolution studies in phosphate buffer at pH 6.8 revealed that granules of trial-2B released only 34% ± 3 DHA in two minutes compared with trial-1A (57% ± 2), trial-1B (48% ± 2) and trial-2A (53% ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/coating; Granules/coating; Dihydroartemisinin (DHA)/taste masking; Thermogravimetric analysis/medicines analysis; X-ray diffraction/medicines analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200013

Circulating cell-free DNA as a biomarker in the diagnosis and prognosis of colorectal cancer
Provedor de dados: 60
Autores: Oliveira,Isadora Bernardo David de; Hirata,Rosario Dominguez Crespo.
Abstract Colorectal cancer (CRC) is a disease without evident clinical symptoms in early stages, leading to late diagnosis and disease management. Current diagnostic and prognostic tools require invasive procedures and circulating molecular biomarkers fail to have optimal sensitivity and specificity. Circulating biomarkers with high clinical performance may be valuable for early diagnosis and prognosis of CRC. The purpose of this review was to investigate the application of circulating cell-free DNA (ccfDNA) in CRC diagnosis and prognosis and the analytical methods used in blood samples in articles published between 2005 and 2016. Based on specific inclusion and exclusion criteria, 26 articles were selected. Most studies used ccfDNA quantification as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal Neoplasms/diagnosis; Colorectal cancer; Circulating cell-free DNA; CcfDNA; Prognosis; Diagnosis; Biomarkers; Neoplastic Cells/circulating.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100406

Antinociceptive local effect of the combination of dexketoprofen trometamol and chlorhexidine gluconate in a formalin test: an additive effect
Provedor de dados: 60
Autores: Esparza-Villalpando,Vicente; Chavarria-Bolaños,Daniel; Zapata-Morales,Juan Ramón; Vértiz-Hernández,Antonio; Pozos-Guillén,Amaury.
ABSTRACT A drug delivery system (DDS) with analgesic and antibacterial properties would be desirable for the local control of post-operatory pain and the prevention for surgical site infection (SSI). The objective of the present study was to evaluate the antinociceptive effect of the combination between dexketoprofen trometamol (DXT) and chlorhexidine gluconate (CHX) in the formalin pain model. Different doses of CHX were combined with DXT and were locally administered in rats paw simultaneously with 5% formalin dilution. Flinches were documented and the antinociceptive effect was calculated. The area under the curve of each experimental group were calculated and the % of antinociception were compared. The groups of CHX and DXT showed similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antinociception/effects; Formalin test; Dexketoprofen trometamol; Chlorhexidine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200601

A novel dosage form for buccal administration of bupropion
Provedor de dados: 60
Autores: Almeida,Nilsa Maria Galvão; Lima,Renata; Alves,Thaís Francine Ribeiro; Rebelo,Márcia de Araújo; Severino,Patrícia; Chaud,Marco Vinícius.
Bupropion is an antidepressant used in the treatment of smoking. The purpose of this study was to prepare controlled-release hydrogel films for buccal administration of bupropion and investigate its physicochemical and cytotoxic properties. The films were prepared from ultrapure sodium carboxymethylcellulose, hydroxypropylmethylcellulose K4M, and medium-viscosity chitosan. Evaluation of film physicochemical characteristics was based on scanning electron microscopy, bupropion content, mechanical strength (burst strength, relaxation, resilience, and traction), and cytotoxicity. Bupropion content in bilayer films was 121 mg per 9 cm2. The presence of bupropion modified film mechanical strength, but did not compromise the use of this pharmaceutical form. As...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bupropion/buccal administration; Bilayer films/drugs rellease; Drugs/controlled-release; Tobacco/control.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100091

The effect of Cratylia floribunda lectin on renal hemodynamics and ion transport
Provedor de dados: 60
Autores: Havt,Alexandre; Assreuy,Ana Maria Sampaio; Nascimento,Nilberto Robson Falcão do; Fonteles,Manassés Claudino; Pereira,Lívia de Paula; Monteiro,Sandra Maria Nunes; Barbosa,Paulo Sergio Ferreira; Nascimento,Kyria Santiago do; Cavada,Benildo Sousa; Martins,Alice Maria Costa; Monteiro,Helena Serra Azul.
Lectins have been described as glycoproteins that reversibly and specifically bind to carbohydrates. Legume lectins isolated from the subtribe Diocleinae (Canavalia, Dioclea andCratylia) are structurally homologous with respect to their primary structures. The Diocleinae lectins of Canavalia brasiliensis, Dioclea guianensis andCanavalia ensiformis have been shown to distinctly alter physiological parameters in isolated rat kidneys. Thus, the aim of this study was to investigate the effect of Cratylia floribunda lectin (CFL) on renal hemodynamics and ion transport in rats. In isolated perfused kidneys, CFL (10 mg/mL, n=5) increased RPP, RVR and decreased %TK+, but did not change urinary flow, glomerular filtration rate, sodium or chloride tubular transport....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant lectin; Amiloride; Cratylia floribunda/lectina/renal hemodynamics; Renal hemodynamics; Ion-transport.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300755

Electrochemical biosensors in pharmaceutical analysis
Provedor de dados: 60
Autores: Gil,Eric de Souza; Melo,Giselle Rodrigues de.
Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosensors; Pharmaceutical analysis; Electrochemical sensors; Enzymes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300002

Prescrição e preparo de medicamentos sem formulação adequada para crianças: um estudo de base hospitalar
Provedor de dados: 60
Autores: Costa,Patrícia Quirino da; Lima,Janete Eliza Soares de; Coelho,Helena Lutéscia Luna.
Este trabalho teve como objetivo identificar medicamentos cuja forma ou formulação farmacêutica representa um problema em pediatria (Medicamento Problema - MP), bem como analisar as estratégias empregadas pelos médicos, para sua utilização nas crianças e os riscos envolvidos. Trata-se de um estudo descritivo, que tem como base um inquérito com pediatras de um hospital de referência do SUS em Fortaleza-Ceará, conduzido para identificação dos MPs em julho-agosto de 2004; uma análise das prescrições contendo adaptação de formas sólidas e uma observação direta do preparo dos medicamentos, que foram conduzidas em dezembro de 2004 e janeiro de 2005, respectivamente. Os medicamentos foram agrupados pela classificação ATC e pelo cálculo de frequências das...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prescrição pediátrica; Formulações farmacêuticas/adaptações para uso pediátrico; Medicamentos/uso pediátrico; Farmácia hospitalar.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100007

Biopharmaceutics classification system: importance and inclusion in biowaiver guidance
Provedor de dados: 60
Autores: Arrunátegui,Lorena Barbosa; Silva-Barcellos,Neila Márcia; Bellavinha,Karime Rezende; Ev,Lisiane da Silveira; Souza,Jacqueline de.
Pharmacological therapy is essential in many diseases treatment and it is important that the medicine policy is intended to offering safe and effective treatment with affordable price to the population. One way to achieve this is through biowaiver, defined as the replacement of in vivo bioequivalence studies by in vitro studies. For biowaiver of new immediate release solid oral dosage forms, data such as intestinal permeability and solubility of the drug are required, as well as the product dissolution. The Biopharmaceutics Classification System (BCS) is a scientific scheme that divides drugs according to their solubility and permeability and has been used by various guides as a criterion for biowaiver. This paper evaluates biowaiver application,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/biowaiver; Biopharmaceutics Classification System; Drugs/solubility; Drugs/permeability; Drugs/dissolution; Drugs/legislation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100143

Synthesis, antileishmanial activity and QSAR studies of 2-chloro- N -arylacetamides
Provedor de dados: 60
Autores: Lavorato,Stefânia Neiva; Duarte,Mariana Costa; Andrade,Pedro Henrique Rocha De; Coelho,Eduardo Antonio Ferraz; Alves,Ricardo José.
ABSTRACT We describe herein the synthesis and evaluation of the antileishmanial activity against promastigote forms of Leishmania amazonensis and cytotoxicity to murine macrophages of a series of 2-chloro-N-arylacetamide derivatives. All compounds were active, except one (compound 3). Compound 5 presented the most promising results, showing good antileishmanial activity (CI50=5.39±0.67 µM) and moderate selectivity (SI=6.36), indicating that further development of this class is worthwhile. Preliminary QSAR studies, although not predictive, furnished some insights on the importance of electronic character of aryl substituent to biological activity, as well as an indirect influence of hydrophobicity on activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis/treatment; Chloroacetamides; Leishmania amazonensisChloroacetamides/QSAR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100620

Paclitaxel and spirulina co-loaded polymeric nanoparticles: in-vitro and in-vivo anticancer study
Provedor de dados: 60
Autores: Ravikumar,Yamuna; Catherine,Dorcas Pinky; Nair,Keerthi G. S; Sekar,Akshya; Velmurugan,Ramaiyan.
Paclitaxel spirulina nanoparticles were said to have promising anticancer activity against gastric cancer. Nanoparticles of paclitaxel-spirulina were prepared for treating gastric cancer using precipitation technique. The synergistic anticancer efficiency againstMKN45 cells retains when the paclitaxel and spirulina were encapsulated into nanoparticles. To increase the site specific delivery, intra-tumoral administration was carried in the in vivo evaluation. There was an increase in overall survival in an MKN45-transplanted mice model and notable improvement in anti-tumour efficacy when paclitaxel-spirulina nanoparticles were delivered through intra-tumoral administration. The further investigation of overall anticancer mechanism of these nanoparticles is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paclitaxel; Spirulina; Nanoparticle; In-vitro; In-vivo; Gastric cancer.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100642

Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
Provedor de dados: 60
Autores: Sobreira,Flávia; Hernandes,Leandro Santoro; Vetore-Neto,Alberto; Díaz,Ingrit E. Collantes; Santana,Fernanda Carvalho de; Mancini-Filho,Jorge; Bacchi,Elfriede Marianne.
ABSTRACT Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric ulcer/study/rats; Gastric ulcer/treatment; Kalanchoe pinnata/chromatographic profile; Kalanchoe pinnata/gastroprotective activity; Quercetrin; Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100616

A stability-indicating HPLC-PDA method for the determination of ferulic acid in chitosan-coated poly(lactide-co-glycolide) nanoparticles
Provedor de dados: 60
Autores: Lima,Isabela Angeli de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.
ABSTRACT The development and validation of a simple and efficient method for the quantification of ferulic acid in poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles coated with chitosan (CS) by reverse phase high performance liquid chromatography coupled to photodiode array detection was described. For the chromatographic analysis, a reverse phase C-18 column was used, mobile phase consisting of acetonitrile and 0.5% acetic acid (37:63, v/v), isocratically eluted at a flow rate of 1 mL/min. Drug determination was performed at 320 nm. The method was validated in terms of the selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification. The method was linear in the range of 10 to 100 μg/mL (r=0.999) and presented limit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Ferulic acid/encapsulation efficiency; Ferulic acid/quantification; Stability; High performance liquid chromatography/validation; PLGA-poly(lactide-co-glycolide)/nanoparticles; Chitosan.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200627

Freeze-drying microscopy in mathematical modeling of a biomaterial freeze-drying
Provedor de dados: 60
Autores: Borgognoni,Camila Figueiredo; Bevilacqua,Joyce da Silva; Pitombo,Ronaldo Nogueira de Moraes.
Transplantation brings hope for many patients. A multidisciplinary approach on this field aims at creating biologically functional tissues to be used as implants and prostheses. The freeze-drying process allows the fundamental properties of these materials to be preserved, making future manipulation and storage easier. Optimizing a freeze-drying cycle is of great importance since it aims at reducing process costs while increasing product quality of this time-and-energy-consuming process. Mathematical modeling comes as a tool to help a better understanding of the process variables behavior and consequently it helps optimization studies. Freeze-drying microscopy is a technique usually applied to determine critical temperatures of liquid formulations. It has...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freeze-drying microscopy; Bovine pericardium; Mathematical modeling.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200003

Prodrugs available on the Brazilian pharmaceutical market and their corresponding bioactivation pathways
Provedor de dados: 60
Autores: Parise Filho,Roberto; Polli,Michelle Carneiro; Barberato Filho,Silvio; Garcia,Monique; Ferreira,Elizabeth Igne.
The aim of this paper was to emphasize the importance of prodrug design to therapy, by examining examples available on the Brazilian pharmaceutical market. The principles of prodrug design are briefly discussed herein. Examples of prodrugs from many important therapeutic classes are shown and their advantages relative to the drugs they are derived from are also discussed. Considering the importance of these therapeutic classes, from both therapy and economic standpoints, prodrug design is a very valuable aspect in the research of new drugs and for the pharmaceutical industry as a whole.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prodrug design; Molecular modification; Drug development.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300003

Official monitoring of the Brazilian Norm for Commercialization of Food for Nursling and Children of First Infancy, Rubber Nipples, Pacifiers, and Nursing Bottles - NBCAL
Provedor de dados: 60
Autores: Bartolini,Fabrício Luiz Silva; Amaral,Maria da Penha Henriques do; Vilela,Miriam Aparecida Pinto; Mendonça,Alessandra Ésther de; Vilela,Fernanda Maria Pinto; Amaral,Lílian Henriques do; Gomes,Felipe Villela.
This work aimed to evaluate advertisement of baby food, rubber nipples, pacifiers and nursing bottles through newspapers as well as on TV, radio, and the internet, in order to check whether the 'Brazilian Norm for Commercialization of Food for Nursling and Children of First Infancy, Rubber Nipples, Pacifiers and Nursing Bottles' (Norma Brasileira de Comercialização de Alimentos para Lactentes e Crianças de Primeira Infância, Bicos, Chupetas e Mamadeiras - NBCAL) has been complied. Samples of all the items above were acquired at discount and department stores to check whether labeling was in compliance with the NBCAL. The development of this research as well as the analyses of commercial promotion were carried out in Juiz de Fora - state of Minas Gerais,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Publicity/baby food; Publicity/evaluation; Advertisement/baby food; Advertisement/evaluation; Childish ware/evaluation of labeling.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300013

Virulence potential of filamentous fungi isolated from poultry barns in Cascavel, Paraná, Brazil
Provedor de dados: 60
Autores: Taira,Cleison Ledesma; Mota,Valdeci Aparecido; Svidzinski,Terezinha Inez Estivalet.
Opportunistic fungi are those that normally would not cause diseases in otherwise healthy people, but are able to cause problems under some circumstances, and for this they need to possess a certain virulence potential. The objective of this study was to identify samples of filamentous fungi isolated from poultry barns in Cascavel, Paraná, and also to evaluate their virulence potential by assessing proteinase production, hemolytic activity, urease production, and growth rate at 37 ºC. We have evaluated the following samples: Acremonium hyalinulum (1 sample), Aspergillus sp. (12), Beauveria bassiana (1), Curvularia brachyspora (1), Paecilomyces variotti (1), and Penicillium sp. (2). Out of the 18 samples analyzed, 44.4% showed proteolytic activity using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filamentous fungi/identification; Filamentous fungi/virulence potential; Filamentous fungi/enzymatic activity; Filamentous fungi/hemolytic activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100019

Early weaning impairs body composition in male mice
Provedor de dados: 60
Autores: Borges,Maria Carolina; Rogero,Marcelo Macedo; Pires,Ivanir Santana de Oliveira; Borelli,Primavera; Tirapegui,Julio.
This study aimed to evaluate the effect of early weaning on body composition and on parameters related to nutritional status in mice. The experimental group consisted of male Swiss Webster mice that were weaned early (at postnatal day fourteen) and fed an appropriate diet for growing rodents until postnatal day twenty-one (EW group). The control group consisted of male mice breastfed until postnatal day twenty-one (CON group). All animals were sacrificed on the twenty-first day of life. The EW group showed a decrease in liver and muscle protein content and concentration, brain protein concentration, brain DNA content and concentration, as well as liver and muscle protein/RNA ratio (p&lt;0.05). Concerning body composition, the EW mice showed increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Weaning; Body composition; Breastfeeding; Nutritional status.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400026

Determination of Gemifloxacin in human plasma by high performance liquid chromatography using Ultra Violet detector and its application to a bioequivalence study
Provedor de dados: 60
Autores: Mousavi,Syed Husain Hashemi.
A liquid chromatography method was developed and validated for the determination of gemifloxacin in human plasma using chloramphenicol as internal standard to achieve lower quantification limit. Acetonitrile was used to precipitated and extracted analyte and internal standard from plasma by Protein Precipitation. Analysis was performed isocratically on C18 column using 25% acetonitrile and 75% 0.02 M phosphate buffer as mobile phase. The method was demonstrated to be linear from 0.003 µg/mL to 5 µg/mL with the lower limit of quantitation of 0.003 µg/mL. The method was successfully applied for the bioequivalence study of gemifloxacin after a single oral administration of 320 mg gemifloxacin mesylate tablets to 12 healthy volunteers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gemifloxacin/determination; Bioequivalence; Plasma; HPLC.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400610

Synthesis of new 2-{2,3-dihydro-1,4-benzodioxin-6-yl[(4-methylphenyl) sulfonyl]amino}-N-(un/substituted-phenyl)acetamides as α-glucosidase and acetylcholinesterase inhibitors and their in silico study
Provedor de dados: 60
Autores: Abbasi,Muhammad Athar; Riaz,Sajid; Rehman,Aziz-ur-; Siddiqui,Sabahat Zahra; Shah,Syed Adnan Ali; Ashraf,Muhammad; Lodhi,Muhammad Arif; Khan,Farman Ali.
The aim of the present research work was to investigate the enzyme inhibitory potential of some new sulfonamides having benzodioxane and acetamide moieties. The synthesis was started by the reaction of N-2,3-dihydrobenzo[1,4]-dioxin-6-amine (1) with 4-methylbenzenesulfonyl chloride (2) in the presence of 10% aqueous Na2CO3 to yield N-(2,3-dihydrobenzo[1,4]-dioxin-6-yl)-4-methylbenzenesulfonamide (3), which was then reacted with 2-bromo-N-(un/substituted-phenyl)acetamides (6a-l) in DMF and lithium hydride as a base to afford various 2-{2,3-dihydro-1,4-benzodioxin-6-yl[(4-methylphenyl)sulfonyl]amino}-N-(un/substituted-phenyl)acetamides (7a-l). All the synthesized compounds were characterized by their IR and 1H-NMR spectral data along with CHN analysis data....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benzodioxane; Acetamide; Spectral analysis; A-Glucosidase; Acetylcholinesterase; Molecular docking.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100518

The importance of corrosion resistance test of cannulas in the quality control of hypodermic needles
Provedor de dados: 60
Autores: Feitoza-Silva,Michele; Carvalho,Carolina Mesquita de; Bittencourt,Lívia Bretas; Leandro,Katia Christina; Gemal,André Luis.
According to Anvisa risk rating, hypodermic needles offer medium risk to the user’s health. This study discussed the importance of the corrosion resistance test in tubes of hypodermic needles, in the product quality control. A review of cannulas of hypodermic needles was carried out according to ISO 9626:2003 and 9259:1997 ABNT NBR. For the results evaluation, a scale which classifies the extent of corrosion was adjusted. 174 samples of PNI needles from 17 States and 9 different record holders were analyzed. According to the methodology of ISO 9626:2003, 100% of the samples were considered satisfactory. However, in accordance with the methodology of ISO 9259:1997, 97.1% of the samples were rejected. Irregularities can lead to impairment of product quality,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corrosion; Hypodermic needles; Health surveillance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100565

PLGA-corosolic acid implants for potential application in ocular neovascularization diseases
Provedor de dados: 60
Autores: Toledo,Cibele Rodrigues; Pereira,Vinícius Viana; Andrade,Gracielle Ferreira; Silva-Cunha,Armando.
Angiogenesis is the formation of new blood vessels from preexisting vasculature. Uncontrolled angiogenesis is associated with progression of several ocular pathologies, such as diabetic retinopathy and macular degeneration. Thus, the inhibition of this process consists in an interesting therapeutic target. Corosolic acid (CA) is a natural derivative of ursolic acid, found in many medicinal herbs and exhibits numerous biological properties, including the antiangiogenic activity. The present study reports the production of CA-loaded poly d,l-lactidecoglycolide acid (PLGA) devices by melt technique. HPLC-UV method was developed and validated to evaluate the uniformity and the release profile of the developed systems. The devices were also characterized by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corosolic acid; PLGA; Antiangiogenic activity; Implant.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100569

Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms
Provedor de dados: 60
Autores: Kalyani,Lella; Rao,Chava Venkata Nageswara.
A simple, accurate, isocratic stability indicating RP-HPLC method was developed for the determination of cefepime and amikacin in Pure and its pharmaceutical formulations. The method consists of methanol: acetonitrile:acetate buffer 75:20:05 (v/v) mobile phase at pH 5.1 with C18 column as stationary phase. The flow rate and detection wave length were 1.0 mL/min and 212 nm respectively. The linearity range for the method was found to be 2.5-25 µg/mL for amikacin and 10-100 µg/mL cefepime respectively. The developed method was validated as per ICH guidelines and the results of all the validation parameters were well within their acceptance values. Also the forced degradation studies were conducted with standard drugs. Degradation products formed during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cefepime/method development and validation; Amikacin/method development and validation; RP-HPLC.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300607

In vitro evaluation of cutaneous penetration of acyclovir from semisolid commercial formulations and relation with its effective antiviral concentration
Provedor de dados: 60
Autores: Sponchiado,Rafaela Martins; Cordenonsi,Leticia Malgarim; Wingert,Nathalie Ribeiro; Araujo,Bibiana Verlindo de; Volpato,Nadia Maria.
ABSTRACT The evaluation of drug permeation/penetration of semisolid formulations into animal skin can be useful to supplement the pharmaceutical equivalence. This paper describes the in vitro assessment of acyclovir (ACV) into porcine skin from commercial formulations with etermination of drug concentration in different layers of cutaneous tissue to correlate with effective antiviral concentration in order to improve the equivalence decision. Studies were conducted using Franz cells and porcine skin. Selected pharmaceutical creams containing ACV had identical (reference and generic) and different (similar) excipients. A software program was employed for the simulation of antiviral effectiveness in the skin. Regarding ACV skin penetration, the first batch...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acyclovir/skin penetration/in vitro studies; Acyclovir/semisolid formulations; Acyclovir/pharmaceutical equivalence.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300483

PAK6: a potential anti-cancer target
Provedor de dados: 60
Autores: Gong,Chan-Chan; Li,Tong-Tong; Pei,Dong-Sheng.
p21-activated kinase 6 (PAK6) is a member of the PAK family of serine/threonine kinases that are known effectors of Rho GTPases Cdc42 and Rac. PAKs regulate a large number of complex cellular mechanisms, including cell motility, morphology, and tumor development. PAK6, initially cloned as an interacting partner of the androgen receptor (AR), is associated with an array of cellular processes implicated in tumor progression. However, the full biological implications of PAK6 activity during cancer remain poorly understood. In this review, we assess our current understanding of the physiological roles of classical PAK6 functionality in mammals, in addition to its emerging role in tumorigenesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PAK6; Androgen Receptor (AR); Cancer therapy; MiRNAs.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100700

Celebrating the 70th Anniversary with increased international visibility!
Provedor de dados: 60
Autores: Ferreira,Elizabeth Igne.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100001

N-Myristoyltransferases as antileishmanial targets: a piggyback approach with benzoheterocyclic analogues
Provedor de dados: 60
Autores: Junqueira,Luis Otávio; Costa,Marcela Oliveira Legramanti da; Rando,Daniela Gonçales Galasse.
Leishmaniasis is one of the neglected diseases that remain in need for pharmacological alternatives. In this context, N-Myristoyltransferases (NMT) arise as interesting targets to explore since they are involved in the co/post-translational processing of peptides which are responsible for host cell invasion. Studies that consider these enzymes as targets point out the potential of benzoheterocyclic compounds as inhibitors of Candida albicans’s N-myristoyltransferase. Here we applied a combination of comparative binding site analysis and molecular docking studies based on a Piggyback approach in the search for new Leishmania major NMT ligands. Our results revealed that NMT enzymes from both pathogens present enough structural similarity to allow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N-myristoyltransferase; Leishmaniasis; Benzofuran; Benzothiazole; Molecular docking; Leishmania major.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100562

Influence of binder type and process parameters on the compression properties and microbial survival in diclofenac tablet formulations
Provedor de dados: 60
Autores: Ayorinde,John Oluwasogo; Itiola,Oludele Adelanwa; Odeku,Oluwatoyin Adepeju; Odeniyi,Michael Ayodele.
The influence of binder type and process parameters on the compression properties and microbial survival in diclofenac tablet formulations were studied using a novel gum from Albizia zygia. Tablets were produced from diclofenac formulations containing corn starch, lactose and dicalcium phosphate. Formulations were analyzed using the Heckel and Kawakita plots. Determination of microbial viability in the formulations was done on the compressed tablets of both contaminated and uncontaminated tablets prepared from formulations. Direct compression imparted a higher plasticity on the materials than the wet granulation method. Tablets produced by wet granulation presented with a higher crushing strength than those produced by the direct compression method....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tablets/production; Tablets/microbial analysis; Direct compression/tablets production; Wet granulation/tablets production; Tablets/microbial contamination; Microbial survival/in tablets; Albizia gum/use; Albizia gum/tablets production.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400022

Rapid titrimetric and spectrophotometric determination of ofloxacin in pharmaceuticals using N-bromosuccinimide
Provedor de dados: 60
Autores: Vinay,Kanakapura Basavaiah; Revanasiddappa,Hosakere Doddarevanna; Devi,Okram Zenita; Ramesh,Pavagada Jagannathamurthy; Basavaiah,Kanakapura.
One titrimetric and two spectrophotometric methods have been described for the determination of ofloxacin (OFX) in bulk drug and in tablets, employing N-Bromosuccinimide as an analytical reagent. The proposed methods involve the addition of a known excess of NBS to OFX in acid medium, followed by determination of unreacted NBS. In titrimetry, the unreacted NBS is determined iodometrically, and in spectrophotometry, unreacted NBS is determined by reacting with a fixed amount of either indigo carmine (Method A) or metanil yellow (Method B). In all the methods, the amount of NBS reacted corresponds to the amount of OFX. Titrimetry allows the determination of 1-8 mg of OFX and the calculations are based on a 1:5 (OFX:NBS) reaction stoichiometry. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ofloxacin/determination; Titrimetry/quantitative analysis; Spectrophotometry/quantitative analysis; N-Bromosuccinimide; Tablets/quantitative analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200006

A nanoemulsion-based delivery system for imatinib and in vitro anticancer efficacy
Provedor de dados: 60
Autores: Karimi,Maryam; Karimian,Khashayar; Heli,Hossein.
A self-nanoemulsifying drug delivery system (SNEDDS) composed of ethyl oleate, Tween 80 and polyethylene glycol 600 was prepared as a new route to improve the efficacy of imatinib. The drug-loaded SNEDDS formed nanodroplets of ethyl oleate stabilized by Tween 80 and polyethylene glycol 600 with a diameter of 81.0±9.5 nm. The nanoemulsion-based delivery system was stable for at least two months, with entrapment efficiency and loading capacity of 16.4±0.1 and 48.3±0.2%, respectively. Imatinib-loaded SNEDDS was evaluated for the drug release profiles, and its effectiveness against MCF-7 cell line was investigated. IC50 values for the imatinib-loaded SNEDDS and an imatinib aqueous solution were 3.1 and 6.5 µg mL-1, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glivec; Nanoemulsion; Anticancer drug; Bioavailability; Breast cancer cell.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100640

Functionality of innovative and generic celluloses in metronidazole formulations
Provedor de dados: 60
Autores: Díaz Ramírez,Carmen Cristina; Villafuerte Robles,Leopoldo.
The wide variety of excipients available calls for evaluation of their functionality, in this case of the pharmaceutical performance of microcrystalline celluloses and sodium cross-linked carboxymethylcelluloses from different sources. This evaluation includes parameters such as powder flow, compactibility, ejection pressure and dissolution from fast-release tablets as well as from floating granules and controlled-release tablets. In a previous study, the excipient Carmacel® presented better disintegration properties compared to Croscarmellose®. However, the evaluation showed better compactibility performance for Croscarmellose®. These characteristics were observed using pure excipients. Nevertheless, these advantages have not been confirmed in tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Excipients/functionality; Microcrystalline celluloses; Sodium cross-linked carboxymethylcelluloses; Croscarmellose; Carmacel; Avicel; Alfacel; Tablets/disintegrants; Pharmaceutical dosage/forms.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100006

Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors
Provedor de dados: 60
Autores: Silva,Aline Ramos da; Tomotani,Ester Junko; Vitolo,Michele.
Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sucrose/conversion; Fructose; Gluconic acid; Invertase; Glucose oxidase; Catalase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200022

Inhibition of LDL-oxidation and antioxidant properties related to polyphenol content of hydrophilic fractions from seaweed Halimeda Incrassata (Ellis) Lamouroux
Provedor de dados: 60
Autores: Costa-Mugica,Ariana; Batista- Gonzalez,Ana Elsa; Mondejar,Diadelis; Soto-López,Yosdel; Brito-Navarro,Victor; Vázquez,Ana Maria; Brömme,Dieter; Zaldívar-Muñoz,Claudina; Vidal-Novoa,Alexis; Silva,Ana Mara de Oliveira e; Mancini-Filho,Jorge.
LDL oxidation and oxidative stress are closely related to atherosclerosis. Therefore, natural antioxidants have been studied as promising candidates. In the present study, the LDL oxidation inhibition activity of bioactive compounds from Halimeda incrassata seaweed. associated to antioxidant capacity, was evaluated in vitro. Experimental work was conducted with lyophilized aqueous extract and phenolic-rich fractions of the seaweed and their effect on LDL oxidation was evaluated using heparin-precipitated LDL (hep-LDL) with exposure to Cu2+ ions and AAPH as the free radical generator. H. incrassata had a protective effect for hep-LDL in both systems and the presence of phenolic compounds contributed to the activity where phenolic-rich fractions showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Halimeda incrassata/antioxidant properties; Antioxidants; Polyphenols; Lipoproteins/oxidation inhibition.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100004

Development of enteric coated sustained release minitablets containing mesalamine
Provedor de dados: 60
Autores: Souza,Dayse Fernanda de; Goebel,Karin; Andreazza,Itamar Francisco.
The aim of this study was to develop and evaluate a multiparticulate modified release system, composed of minitablets with a sustained release matrix system coated with a pH-dependent release polymer, using mesalamine as a model drug. Polyox® WSR 1105 was the polymer used in the matrix system and Eudragit® L30D55 was used as a pH-dependent polymer. The minitablets (with 20%, 30% or 40% Polyox® concentration) were prepared by dry granulation, which led to good quality minitablets. The developed minitablets were coated in a fluidized bed at 8% of the coating level. Dissolution studies were performed in media that simulated the gastrointestinal tract (pH 1.4, 6.0 and 7.2) and showed that formulations with higher Polyox® concentrations were capable of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesalamine/modified release; Mesalamine/evaluation; Minitablets coated/modified release; Minitablets coated/evaluation; Polyethylene oxide/drugs coating; Drugs/modified release.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300014

Development of nanotechnology-based drug delivery systems with olive vegetable oil for cutaneous application
Provedor de dados: 60
Autores: Silva,Silas Arandas Monteiro e; Lacerda,Rísia; Bernegossi,Jéssica; Chorilli,Marlus; Leonardi,Gislaine Ricci.
ABSTRACT Liquid-Crystalline Systems represent active compounds delivery systems that may be able to overcome the physical barrier of the skin, especially represented by the stratum corneum. To obtain these systems, aqueous and oily components are used with surfactants. Of the different association structures in such systems, the liquid-crystalline offer numerous advantages to a topical product. This manuscript presents the development of liquid-crystalline systems consisting, in which the oil component is olive oil, its rheological characterizations, and the location of liquid crystals in its phase map. Cytotoxic effects were evaluated using J-774 mouse macrophages as the cellular model. A phase diagram to mix three components with different proportions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liquid-Crystalline Systems/development; Liquid-Crystalline Systems/reology; Drugs/skin delivery; Olive Oil/use/Liquid-Crystalline Systems; Rheology; Cytotoxicity; Bioadhesion.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100023

Simvastatin treatment reduces the cholesterol content of membrane/lipid rafts, implicating the  N -methyl-D-aspartate receptor in anxiety: a literature review
Provedor de dados: 60
Autores: Cruz,Júlia Niehues da; Magro,Débora Delwing Dal; Lima,Daniela Delwing de; Cruz,José Geraldo Pereira da.
ABSTRACT Membrane/lipid rafts (MLRs) are plasmalemmal microdomains that are essential for neuronal signaling and synaptic development/stabilization. Inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme-A reductase (statins) can disable the N-methyl-D-aspartate (NMDA) receptor through disruption of MLRs and, in turn, decrease NMDA-mediated anxiety. This hypothesis will contribute to understanding the critical roles of simvastatin in treating anxiety via the NMDA receptor.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiolytic effects; Membrane/lipid rafts; N-methyl-D-aspartate; Simvastatin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100401

Total polyphenol content and antioxidant activity of commercial Noni (Morinda citrifolia L.) juice and its components
Provedor de dados: 60
Autores: Bramorski,Adriana; Cherem,Adriana da Rosa; Marmentini,Chaiana Paula; Torresani,Joseane; Mezadri,Tatiana; Costa,Andréa de Almeida Silva.
The plant Morinda citrifolia L. (noni) has been the focus of many recent studies due to its potential effects on treatment and prevention of several diseases. However, there are few in vivo and in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine the total polyphenol content (TPC) and antioxidant capacity of a juice commercialized as noni juice, but containing grape, blueberry and noni fruits. Commercial noni juice was compared against its separate constituents of blueberry and grape juice. Folin-Ciocalteu and DPPH• methods were used to determine the concentration of total polyphenol content and antioxidant activity, respectively. Commercial noni juice presented higher values of TPC (91.90 mg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Noni juice/antioxidant activity; Morinda citrifolia L.; Total polyphenols/determination; Antioxidants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400006

Fabrication and in vitro characterization of polymeric nanoparticles for Parkinson's therapy: a novel approach
Provedor de dados: 60
Autores: Gulati,Neha; Nagaich,Upendra; Saraf,Shubhini.
The objective of the research was to formulate and evaluate selegiline hydrochloride loaded chitosan nanoparticles for the Parkinson's therapy in order to improve its therapeutic effect and reducing dosing frequency. Taguchi method of design of experiments (L9 orthogonal array) was used to get optimized formulation. The selegiline hydrochloride loaded chitosan nanoparticles (SHPs) were prepared by ionic gelation of chitosan with tripolyphosphate anions (TPP) and tween 80 as surfactant. The SHPs had a mean size of (303.39 ± 2.01) nm, a zeta potential of +32.50mV, and entrapment efficiency of SHPs was 86.200 ± 1.38%. The in vitro drug release of SHPs was evaluated in phosphate buffer saline (pH 5.5) using goat nasal mucosa and found to be 82.529% ± 1.308 up...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitosan/nanoparticles/evaluation; Chitosan/nanoparticles/formulation; Selegiline hydrochloride/nanoparticles/drug release; Parkinson's therapy; Polymeric nanoparticles/formulation; Polymeric nanoparticles/evaluation; Taguchi Design.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400869

Women cancer prevention and pharmaceutical contribution
Provedor de dados: 60
Autores: Lourenço,Andrezza Viviany.
In Brazil, many cases of breast and cervical cancers are only diagnosed in advanced stages. Information on prevention of cancer in women is increasingly available. However, prevention or early treatment alternatives are often not practiced. This study investigated the issues hindering the practice of prevention against cancer in women. A qualitative method was employed in this exploratory and descriptive study. The sample included thirty-three randomly selected women undergoing treatment. The survey data was collected at the South Parana Institute of Oncology, Ponta Grossa - PR in September 2007 using a semi-structured individual interview after approval by the Research Ethics Committee of the Brazilian College of Systemic Studies - CBES, Curitiba - PR,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care; Cancer/prevention; Breast cancer; Cancer/uterine cervix.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100006

Utilization profile of enoxaparin in hospitalized patients with Chronic Kidney Disease in southern Brazil
Provedor de dados: 60
Autores: Cirne,Carolina Araujo; Machado,Tatiane Araujo Castro.
Chronic Kidney Disease (CKD) patients usually require the use of anticoagulants for treatment or prevention of thromboembolic diseases. One such commonly used anticoagulant is enoxaparin. However, special attention is advised when using this drug for CKD patients because dosage adjustments may be needed. This prospective observational study was conducted in adult patients (&gt; 18 years) with CKD stages 3 to 5 who were followed-up daily via prescriptions, medical records and laboratory tests. Enoxaparin doses, renal function and adverse drug events (ADEs) were thus monitored. Of the 87 ADEs identified in this study, 56 (64.3%) occurred in patients requiring enoxaparin prescription adjustment. Out of the 9 patients who had severe ADEs, 8 (88.9%) needed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low molecular weight heparin; Enoxaparin; Renal disease; Anticoagulation management; Therapeutic drug monitoring; Clinical pharmacists.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100533

Phenolic compounds and antioxidant, antimicrobial and antimycobacterial activities of Serjania erecta Radlk. (Sapindaceae)
Provedor de dados: 60
Autores: Cardoso,Claudia Andréa Lima; Coelho,Roberta Gomes; Honda,Neli Kika; Pott,Arnildo; Pavan,Fernando Rogério; Leite,Clarice Queico Fujimura.
Serjania erecta Radlk.(Sapindaceae) is a medicinal plant traditionally used in Brazil. We assayed the ethanolic extract of leaves and roots against seven microorganisms. The REMA (Resazurin Microtiter Assay) assay was used to measure the biological activity in vitro against Mycobacterium tuberculosis and our results showed moderate activity of the ethanolic extract. On the other hand S. aureus, P. aeruginosa, S. setubal, C. albicans, S. cerevisiae and E. coli revealed that the leaves and roots of S. erecta inhibited the growth of all microorganisms. The ethanolic extracts of leaves and roots showed low values of antioxidant activities. The ethanolic extracts of leaves and roots were analyzed by chromatographic and spectrometric methods. (-)-Epicatechin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serjania erecta Radlk/pharmacognosy; Serjania erecta Radlk/ethanolic extract; Serjania erecta Radlk/antioxidant activity; Serjania erecta Radlk/ethanolic extract; Serjania erecta Radlk/antimicrobial activity; Sapindaceae/pharmacognosy; Cipó-cinco-folhas/pharmacognosy; Flavonoids; Medicinal plants.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400017

Is dialysis water a safe component for hemodialysis treatment in São Paulo State, Brazil?
Provedor de dados: 60
Autores: Hilinski,Ellen Gameiro; Almodovar,Adriana Aparecida Buzzo; Silva,Fernando Pontes de Lima e; Pinto,Terezinha de Jesus Andreoli; Bugno,Adriana.
Failure on the water treatment poses hemodialysis patients at risk of injury and death. Identifying if the patients are exposed to water quality related microbiological risks is an important objective to reduce the mortality for chronic renal patients and is the main issue of this study. We evaluated the microbiological water quality used by 205 dialysis services in São Paulo State, Brazil between 2010 to 2016. The study included heterotrophic bacteria count, total coliforms research, and bacterial endotoxin determination in 1366 dialysis water samples. The number of unsatisfactory clinics for at least one microbiological parameter decreased 16.0% between 2010 to 2015 but increased 57.2% in 2016. In 2010, the most frequent unsatisfactory parameter was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemodialysis water; Endotoxin; Microbiological water quality; Hemodialysis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100542

Development and characterization of pullulan-polymethacrylate free films as potential material for enteric drug release
Provedor de dados: 60
Autores: Lima,Isabela Angeli de; Pomin,Suelen Plaza; Cavalcanti,Osvaldo Albuquerque.
ABSTRACT Free films of pullulan-polymethacrylate associations were produced by casting process to develop a novel target-specific material. For characterization, tests of water vapor permeability, swelling index, infrared absorption spectroscopy, thermogravimetric analysis, scanning electron microscopy and mechanical analysis were performed. The polysaccharide concentration directly influenced vapor permeability and swelling, increasing the values of the latter up to five times when added in a proportion of 20% (per weight). The individual properties of each polymer were maintained, and chemical interactions were not detected. The films were found to be thermally stable and they had unaltered mechanical properties with the addition of the polysaccharide....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Films; Polymeric material; Modified release/enteric release/oral delivery/controlled release; Glucans; Eudragit; Physicochemical characterization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300601

Pharmacobotanical study of Manilkara zapota (L.) P.Royen (Sapotaceae)
Provedor de dados: 60
Autores: Moura,Bruna Izabela de Vasconcelos; Araújo,Bárbara Priscylla Lira de; Sá,Rafaela Damasceno; Randau,Karina Perrelli.
Manilkara zapota (L.) P. Royen, popularly known as sapoti or sapota (sapodilla), is a tree bearing an important fruit, in addition to different parts of the plant being widely used in folk medicine in the management of inflammation, pain, fevers, coughs, diarrhea, dysentery, among other ailments. This study aimed to conduct a pharmacobotany standardization study of M. zapota. Semi-permanent slides, containing transversal sections of stem, petiole, leaf blade and fruit; and paradermic sections of leaf blade were prepared, and analyzed by light microscopy. Histochemical tests were also performed in cross-sections of the leaf blade. Microscopic analysis allowed the identification of important elements in the diagnosis of the species; while the use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Histochemistry; Sapota; Sapoti; Manilkara/drug effects; Sapotaceae/drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100547

Discovery of N-arylsulfonyl-3-acylindole benzoyl hydrazone derivatives as anti-HIV-1 agents
Provedor de dados: 60
Autores: Che,Zhiping; Tian,Yuee; Liu,Shengming; Hu,Mei; Chen,Genqiang.
The discovery and development of novel inhibitors with activity against variants of human immunodeficiency virus type 1 (HIV-1) is pivotal for overcoming treatment failure. As our ongoing work on research of anti-HIV-1 inhibitors, 32 N-arylsulfonyl-3-acylindole benzoyl hydrazone derivatives were prepared by introduction of the hydrazone fragments on the N-arylsulfonyl-3-acylindolyl skeleton and preliminarily screened in vitro as HIV-1 inhibitors for the first time. Among of all the reported analogues, eight compounds exhibited significant anti-HIV-1 activity, especially N-(3-nitro)phenylsulfonyl-3-acetylindole benzoyl hydrazone (18) and N-(3-nitro)phenylsulfonyl-3-acetyl-6-methylindole benzoyl hydrazone (23) displayed the most potent anti-HIV-1 activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benzoyl hydrazone; Human immunodeficiency virus type-1; Inhibitor of virus replication; Anti-HIV-1 agent.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400620

A study on enhanced intestinal permeability of clarithromycin nanoparticles
Provedor de dados: 60
Autores: Zakeri-Milani,Parvin; Islambulchilar,Ziba; Majidpour,Fatemeh; Jannatabadi,Ensieh; Lotfipour,Farzaneh; Valizadeh,Hadi.
The main objective of the present study was to determine the permeability of clarithromycin (CLA)-PLGA nanoparticles using single-pass intestinal perfusion technique in rats. Clarithromycin nanoparticles were prepared by nano-precipitation according to the modified quasi emulsion solvent diffusion technique and evaluated for their physicochemical characteristics. Permeability coefficients (Peff) in anaesthetized rats were determined at 3 different concentrations. Drug solution or suspensions in PBS was perfused through a cannulated jejunal segment and samples were taken from outlet tubing at different time points up to 90 min. Microbiological assay of CLA and phenol red in the samples were analyzed using an agar well diffusion procedure and HPLC method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clarithromycin/nanoparticles/ permeability; Single-Pass Intestinal Perfusion; Intestinal permeability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100012

Laurus nobilis L.: assessment of the cytotoxic and genotoxic potential of aqueous extracts by micronucleus and Allium cepa assays
Provedor de dados: 60
Autores: Silva,Mayara Christine; Matos,Aline Ferreira; Santos,Hataânderson Luíz Cabral dos; Gomes,Jennifer Vieira; Pastura,Danilo Giovanni Narciso; Pereira,Gabriela Lopes; Rocha,Ellen Borges da; Larangeira,Mayara de Jesus Costa; Alves,Raphael Santiago; Bastos,Leonardo de Oliveira; Borba,Helcio Resende; Lima,Viviane Moreira de.
Laurus nobilis L. is a large shrub belonging to the Lauracea family. Its leaves are widely used for food seasoning as well as in folk medicine. Various studies have demonstrated the antiproliferative, antifungal and antibacterial effects of Laurus nobilis, but no studies have investigated the genotoxic effect of the aqueous extract of the plant. The objective of this study was to analyze the genotoxic potential of an aqueous extract of leaves, using the Allium cepa assay and mouse peripheral blood cell micronucleus test. The results showed that the extract did not have any genotoxic activity, but cytotoxic activity was observed in the two experimental models used. The extract had an antiproliferative effect, detected through the reduction of the mitotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laurus nobilis; Allium cepa; Micronucleus; Genotoxicity; Cytotoxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100534

Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms
Provedor de dados: 60
Autores: Azevedo,Pamela Oliveira de Souza de; Molinari,Francesco; Oliveira,Ricardo Pinheiro de Souza.
Abstract Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria, which exhibit a large spectrum of action against spoilage bacteria and foodborne pathogens. Successful application of techniques for quantitative or qualitative bacteriocin determination relies not only on the sensitivity of the test-microorganisms, but also on the agar-medium employed. Cell free supernatants are routinely used to preliminary screen for antimicrobial activity of bacteria by means of the agar well diffusion method, but the supernatant may also include other molecules (such as medium components and/or intracellular compounds) accidentally released during cell free supernatant preparation, which may interfere with the assay....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bacteriocin; Growth inhibition; Solid medium; Agar well diffusion assay.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100301

Carbamide peroxide gel stability under different temperature conditions: is manipulated formulation an option?
Provedor de dados: 60
Autores: Bonesi,Camila de Martini; Ulian,Letícia Storchi; Balem,Paula; Angeli,Valeria Weiss.
Nowadays the use of gel containing carbamide peroxide (CP) prepared in Pharmacy is a normal practice in the population. However, the quality of this product is questionable concerning its stability. The aim of this study is was to synthesize and to analyze this drug alone or associated to Carbopol gel through analytical methodology compatible with the routine of the Pharmacies. The reaction between urea and hydrogen peroxide was carried out at different resting times: 24 hours (CP 24 powder) and 48 hours (CP48 powder) after the mixture. Both products were associated with Carbopol 940® gel 1.5% (G) generating G24 and G48 samples. The stability of powders (CP24 e CP48) and the formulations (G24 and G48) were evaluated as a function of time (15, 40 and 45...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbamide peroxide/quantitative analysis; Carbamide peroxide/stability; Dental bleaching; Carbopol 940® gel 1.5%.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400008

Medication reconciliation as a strategy for preventing medication errors
Provedor de dados: 60
Autores: Spalla,Luana de Rezende; Castilho,Selma Rodrigues de.
ABSTRACT One of the current barriers proposed to avoid possible medication errors, and consequently harm to patients, is the medication reconciliation, a process in which drugs used by patients prior to hospitalization can be compared with those prescribed in the hospital. This study describes the results of a pharmacist based reconciliation conducted during six months in clinical units of a university hospital. Fourteen patients (23.33%) had some kind of problem related to medicine. The majority (80%) of medication errors were due to medication omission. Pharmaceutical interventions acceptance level was 90%. The results suggest that pharmacists based reconciliation can have a relevant role in preventing medication errors and adverse events. Moreover, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medication reconciliation; Medications/errors/ion Medications/adverse events/prevention; Patient safety; Pharmacist/professional performance.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100016

Hepatoprotective effect of Phytosome Curcumin against paracetamol-induced liver toxicity in mice
Provedor de dados: 60
Autores: Tung,Bui Thanh; Hai,Nguyen Thanh; Son,Phan Ke.
Abstract Curcuma longa, which contains curcumin as a major constituent, has been shown many pharmacological effects, but it is limited using in clinical due to low bioavailability. In this study, we developed a phytosome curcumin formulation and evaluated the hepatoprotective effect of phytosome curcumin on paracetamol induced liver damage in mice. Phytosome curcumin (equivalent to curcumin 100 and 200 mg/kg body weight) and curcumin (200 mg/kg body weight) were given by gastrically and toxicity was induced by paracetamol (500 mg/kg) during 7 days. On the final day animals were sacrificed and liver function markers (ALT, AST), hepatic antioxidants (SOD, CAT and GPx) and lipid peroxidation in liver homogenate were estimated. Our data showed that phytosome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin/effects; Curcumin/antioxidant activity; Curcuma longa; Phytosome; Hepatoprotective.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100628

Biological evaluation of 2,3-dioxoindolin-N-phenylacetamide derivatives as potent CDC25B and PTP1B phosphatase inhibitors
Provedor de dados: 60
Autores: Jin,Qing-Hao; Chen,Wen-Bo; Xia,Ya-Nan; Liu,Bing-Yu; Guan,Li-Ping.
A series of 2,3-dioxoindolin-N-phenylacetamide derivatives was evaluated for inhibitory activity against CDC25B and PTP1B enzymes. Most of the derivatives showed inhibitory activity against CDC25B (IC50 = 3.2-23.2 µg/mL) and PTP1B (IC50 = 2.9-21.4 µg/mL). Compound 2h showed the most inhibitory activity in vitro with IC50 values of 3.2 and 2.9 µg/mL against CDC25B and PTP1B, respectively, compared with the reference drugs Na3VO4 (IC50 = 2.7 µg/mL) and oleanolic acid (IC50 = 2.3 µg/mL). The results of selectivity experiments showed that the 2,3-dioxoindolin-N-phenylacetamide derivatives were selective inhibitors against CDC25B and PTP1B. Enzyme kinetic experiments demonstrated that compound 2h was a specific inhibitor with the typical characteristics of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioxoindolin-N-phenylacetamide; CDC25B; PTP1B; Phosphatase inhibitors; Enzyme kinetics.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100500

Freeze-drying of ampicillin solid lipid nanoparticles using mannitol as cryoprotectant
Provedor de dados: 60
Autores: Alihosseini,Faezeh; Ghaffari,Solmaz; Dabirsiaghi,Ali Reza; Haghighat,Setareh.
abstract Solid lipid nanoparticles (SLNs) are interesting colloidal drug-delivery systems, since they have all the advantages of the lipid and polymeric nanoparticles. Freeze-drying is a widely used process for improving the stability of SLNs. Cryoprotectants have been used to decrease SLN aggregations during freeze-drying. In this study Ampicillin was chosen to be loaded in a cholesterol carrier with nano size range. To support the stability of SLNs, freeze-drying was done using mannitol. Particle size, drug release profile and antibacterial effects were studied after freeze-drying in comparison with primary SLNs. Preparations with 5% mannitol showed the least particle size enlargement. The average particle size was 150 and 187 nm before and after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid lipid nanoparticles; Stability; Cryoprotectant; Freeze-drying; Antibacterial efficacy; Particle size.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400797

Determination of the melting temperature, heat of fusion, and purity analysis of different samples of zidovudine (AZT) using DSC
Provedor de dados: 60
Autores: Araújo,Adriano Antunes Souza; Bezerra,Marília dos Santos; Storpirtis,Sílvia; Matos,Jivaldo do Rosário.
The determination of chemical purity, melting range, and variation of enthalpy in the process of characterizing medicines is one of the principal requirements evaluated in quality control of the pharmaceutical industry. In this study, the method of purity determination using DSC was outlined, as well as the application of this technique for the evaluation of commercial samples of zidovudine (AZT) (raw material) supplied by different laboratories. To this end, samples from six different laboratories (A, B, C, D, E, and F) and the standard reference (R) from the United States Pharmacopeia (USP) were analyzed. The DSC curves were obtained in the temperature range of 25 to 200 ºC under the dynamic atmosphere of N2 (50 mL min-1), heating rate of β=2 ºC min-1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zidovudine/purity determination; Differential scanning calorimetry; Thermal analysis; Medicines/quality control; Medicines/qualitative analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100005

Pharmacotherapy review: a proposal to improve medication adherence among hypertensive patients
Provedor de dados: 60
Autores: Rigoni,Cláudia Coelho; Brito,Evelin Soares de; Alano,Graziela Modolon; Galato,Dayani.
Abstract Pharmacotherapy review is a structured assessment of medicines, which aims to obtain a partnership with patients to achieve drug treatment goals and agreement about drug dosage, as well as when and how the drugs should be administered. The objective was to analyze the influence of pharmacotherapy review, by scheduling drug administration to improve medication adherence among antihypertensive patients. This study was an uncontrolled intervention developed in three distinct stages. The first stage included data collection on the profile of patients and their medications, and a preliminary assessment of medication adherence. In the second stage, the review report was delivered to patients. In the third stage, the results of blood pressure and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacotherapy review; Drugs/administration schedule; Hypertension/medication adherence; Medication adherence; Pharmaceutical assistance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400763

BONEFILL ® block as alternative for bone substitute: a toxicological evaluation
Provedor de dados: 60
Autores: Melchior,Karine; Saska,Sybele; Coelho,Fernanda; Scarel-Caminaga,Raquel Mantuaneli; Capote,Ticiana Sidorenko de Oliveira.
ABSTRACT Bone substitutes based on hydroxyapatite (HA) and Bonefill® (BO - inorganic bovine bone) associated with poly(lactic-co-glycolic acid) (PLGA) (HA/PLGA and BO/PLGA) were evaluated concerning cytotoxicity, genotoxicity and mutagenicity as potential candidates for bone repair. The materials were developed and provided by Bionnovation Biomedical Products Ltda. Eluates from these bone substitutes were prepared for toxicity evaluations using eukaryotic cell cultures. HA/PLGA was used as a comparison for Bonefill®. Cell viability was evaluated by XTT assay and surviving fraction was calculated for clonogenic survival. Additionally, tail moment was used to assess genotoxicity (comet assay). The frequencies of binucleated cells with micronucleus (FBMN),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BONEFILL®/toxicity; Biomaterials/evaluation; Bone regeneration; Hydroxyapatite.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200611

The new scenario of drug innovation
Provedor de dados: 60
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300001

Comparative performance of lab tests and blood testing device to monitor glucose, total cholesterol and triacylglycerol in type 2 diabetic patients
Provedor de dados: 60
Autores: Zubioli,Arnaldo; Batista,Marcia Regina; Stefanello,Talitha Fernandes; Carrara,Marcia Aparecida; Bazotte,Roberto Barbosa.
The performance of lab tests (LT) and blood testing devices (BTD) to monitor glycemia vs. glycated hemoglobin A1c (A1c) were compared. In addition, the performance of blood glucose, total cholesterol (TC) and triacylglycerol measured by LT and BDT were compared. All parameters were measured based on the same blood samples from overnight fasted type 2 diabetic patients (T2DP). Linear regression analysis was used for all comparisons. The results showed that A1c correlated better with LT-glucose (r = 0.58) than BTD-glucose (r = 0.42). Moreover, LT vs. BTD showed r values of 0.90, 0.82 and 0.92 for glucose, TC and triacylglycerol, respectively. It was concluded that the performance of LT-glucose was better than BDT-glucose. Moreover, since triacylycerol and TC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laboratory tests; Glycated hemoglobin; Glycemia; Total cholesterol; Triacylglycerol; Type 2 diabetes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300020

Toxic effects of different doses of cyclophosphamide on the reproductive parameters of male mice
Provedor de dados: 60
Autores: Kanno,Tatiane Yumi Nakamura; Sensiate,Lucimara Aparecida; Paula,Natália Aparecida de; Salles,Maria José Sparça.
The cyclophosphamide is used in cancer treatment. The aim of this study was evaluating the effect of different doses of this drug on male mice reproductive parameters. The cyclophosphamide was administered in the doses 100, 150, 200 e 250 mg.kg-1, intraperitoneal route, for six weeks. As a result, it was observed a decrease in body mass and a decrease in testicles and kidney's weight, in all animals treated with cyclophosphamide. Only the groups that received the doses 100, 150 mg.kg-1 of cyclophosphamide were able to fertilize their females. There was higher incidence of post- implantation losses, reabsorptions and decrease in fetal viability in the group that received the dose of 150 mg.kg-1. It was observed a reduction in epididymis and liver's weight...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclophosphamide/effects; Cyclophosphamide/toxicity; Spermatogenesis/experimental study; Chemotherapeutics/adverse effects; Fertility/experimental study.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200017

Simultaneous detection of three antineoplastic drugs on gloves by liquid chromatography with diode array detector
Provedor de dados: 60
Autores: Alcântara,Adélia Maria Pimenta de Pádua; Vitor,Ricardo Vilela; Vieira,Elisabeth Pizzamiglio; Martins,Isarita.
The aim of this study was to develop a method for simultaneous detection of antineoplastic drugs on gloves since, in occupational exposure, the main contamination route is through dermal contact, which may occur via prolonged contact with contaminated surfaces or materials. The assay was performed by liquid chromatography using the following conditions for the detection of 5-fluorouracil (5-FU), methotrexate (MTX) and paclitaxel (TAX): diode array detection and UV quantification at 195 nm for TAX, at 265 nm for 5-FU and at 302 nm for MTX; ODS column (250 x 4 mm, 5 μm) with a similar guard column; mobile phase consisted of water (pH 4)-methanol-acetonitrile (35:15:50, v/v/v) with a flow rate of 1 mL min-1. The method presented a linear range from 0.25 to 20...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antineoplastic drugs/detection; Gloves/toxicologial analysis; High Performance Liquid Chromatography/toxicologial analysis; Occupational exposure.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400015

Preparation of mupirocin-loaded polymeric nanocapsules using essential oil of rosemary
Provedor de dados: 60
Autores: Rubenick,Jaqueline Bandeira; Rubim,Alexandre Machado; Bellé,Francieli; Nogueira-Librelotto,Daniele Rubert; Rolim,Clarice Madalena Bueno.
Abstract The purpose of this study was to prepare and characterize mupirocin-loaded polymeric nanocapsules using two different oils and to develop and validate an analytical method for quantitative determination by high performance liquid chromatography. The mean size of the nanoparticles was 233.05 nm and 275.03 nm for nanocapsules with a rosemary oil like oily core and caprylic/capric triglyceride, respectively, and a good polydispersity index below 0.25 for both formulations. The nanocapsules showed good stability when stored at 40 ºC and room temperature for 30 days. The quantitative method was performed with a mobile phase consisting of ammonium ammonium acetate (0.05 M adjusted to pH 5.0 with acetic acid) and acetonitrile 60:40 (v/v); the flow rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mupirocin/High Performance Liquid Chromatography; Mupirocin/Polymeric nanocapsules; Rosemary oil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100623

Pharmaceutical Sciences is in the agenda!
Provedor de dados: 60
Autores: Ferreira,Elizabeth Igne.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400001

Can mouth washes containing chlorhexidine 0.12% be used as synonym of a water solution of chlorhexidine 0.12%?
Provedor de dados: 60
Autores: Suffredini,Ivana Barbosa; Saraceni,Cintia Helena Coury; Díaz,Ingrit Elida Collantes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouth washes/evaluation Periogard&lt; Sup&gt; ®&lt; /sup&gt; Chlorhexidine Antimicrobials/mouth use/evaluation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200367

Evaluating the discriminatory power of a dissolution assay for rosuvastatin calcium capsules: Solid-state properties and dissolution media
Provedor de dados: 60
Autores: Ângelo,Marilene Lopes; Ruela,André Luís Morais; Ferreira,Anna Clara Marques; Ramos,Marcus Vinicus de Faria; Montanari,Cristina Martiniano; Silva,Lucélia Magalhães da; Araújo,Magali Benjamim de.
We propose to evaluate the dissolution properties of rosuvastatin calcium (ROSC) capsules in different media to characterize the discriminatory power of the assay method. Dissolution assays were performed in media with different pH, and including the surfactant sodium dodecyl sulfate (SDS). Several immediate-release formulations were manufactured using the commercial raw material characterized as amorphous solid. The hydrophobic adjutant magnesium stearate was employed in some formulations due to its negative effect in the wettability and dissolution efficacy of solid dosages. These formulations showed the lower dissolution efficacy values in media without surfactant; however, when SDS was added to the medium, the dissolution efficacy increased, and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dissolution; Crystal polymorphism; Solid dosage form; X-ray powder diffractometry; Thermal analysis; Solubility.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100527

The physical, chemical and functional characterization of starches from Andean tubers: oca (Oxalis tuberosa Molina), olluco (Ullucus tuberosus Caldas) and mashua (Tropaeolum tuberosum Ruiz &amp;amp; Pavón)
Provedor de dados: 60
Autores: Valcárcel-Yamani,Beatriz; Rondán-Sanabria,Gerby Giovanna; Finardi-Filho,Flavio.
The physical, chemical, and functional properties of starches isolated from the Andean tubers oca (Oxalis tuberosa M.), olluco (Ullucus tuberosus C.) and mashua (Tropaeolum tuberosum R. &amp;amp; P.) were studied. The tubers were obtained from a local grocery. The morphology of the starch granules (size and shape) was studied with scanning electron microscopy (SEM), which revealed ellipsoid, oval, conical, pear-shaped and prismatic forms: ellipsoids and oval granules with lengths up to 54.30 µm in oca; with lengths up to 32.09 µm for olluco starch granules; and with predominantly truncated spherical or oval forms and smaller dimensions (up to 16.29 um) for mashua starch granules. Amylose contents were similar among the samples: 27.60% (oca), 26.49%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Andean tubers/physiochemical properties; Andean tubers/functional properties; Tuber starches; Scanning electron microscopy/chemical analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300007

Development and validation of an HPLC-UV method for accelerated stability study and pharmacokinetic analysis of venlafaxine
Provedor de dados: 60
Autores: Sher,Muhammad; Ahmad,Maria; Hassan,Faiza; Naeem-ul-Hassan,Muhammad; Hussain,Muhammad Ajaz.
A reverse phase high performance liquid chromatography method has been developed and validated for accelerated stability study and determination of pharmacokinetic parameters of venlafaxine HCl. The chromatographic separation was carried out using ODS analytical column (250 × 4.6 mm i.d., 5 µm particle size). The mobile phase included acetonitrile, methanol and potassium dihydrogen phosphate buffer (30:30:40; pH 6.1) at a flow rate 1.5 mL min−1. UV-Visible detector was used at wavelength of 227 nm to monitor elutions. Retention time observed was 2.745 min. The method was validated for linearity, accuracy, precision, sensitivity and robustness. Accelerated stability study of venlafaxine HCl capsules was carried out at 40 and 50 ºC under 75% RH level....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venlafaxine; HPLC; Stability; Pharmacokinetics; Chromatography.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100514

Safety assessment of essential medicines for elderly people: a bibliographic survey
Provedor de dados: 60
Autores: Varallo,Fabiana Rossi; Oliveira,Fernanda Mariana de; Mastroianni,Patrícia de Carvalho.
Certain medicines are considered potentially inappropriate (PIM) for elderly people as they increase the risk of adverse drug events (ADE) and because safer alternative therapies are available on the market. In this context, in order to identify the instruments that assess the quality of medical prescriptions for elderly and to determine which drugs are considered PIM, a bibliographic survey was conducted in PUBMED, LILACS and PAHO databases, in February and March/2010. The search strategy included the use of health descriptors and a manual search in the references cited by selected papers. During the period of data collection, 15 instruments were identified. In 2012, with the publication of the update of Beers criteria, this instrument was included in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aged/medicines prescription; Medication/errors; Medicines/inappropriate prescribing; Medicines/adverse effects/control; Medicines/safety use; Medication/risk assessment/control; Pharmaceutical care.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200269

Floating tablets of hydralazine hydrochloride: optimization and evaluation
Provedor de dados: 60
Autores: Vanitha,Kondi; Varma,Mohan; Ramesh,Alluri.
Hydralazine hydrochloride has a half-life of 2 to 4 hours with an oral bioavailability of 26-50%. Since hydralazine has a demethylating effect on various suppressor genes, it can be used in various types of cancer to support chemotherapy. The purpose of this study was to optimize and evaluate floating tablets of hydralazine hydrochloride designed to prolong the gastric residence time and to provide controlled release of the drug for 14 h. The floating tablets of hydralazine hydrochloride were prepared by the wet granulation method. Semi-synthetic polymers of hydroxy propyl methyl cellulose (HPMC K100M) and ethyl cellulose were used as the release retarding agents. A 2² factorial design was applied to systematically optimize the drug release profile. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydralazine/floating tablets; Hydralazine/evaluation; Hydralazine/floating tablets; Hydralazine/optimization; Hydralazine/floating tablets; Hydralazine/controlled release; In vivo X-ray/tablets performance; Cancer/treatment.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400021

Sustainability, natural and organic cosmetics: consumer, products, efficacy, toxicological and regulatory considerations
Provedor de dados: 60
Autores: Fonseca-Santos,Bruno; Corrêa,Marcos Antonio; Chorilli,Marlus.
The interest in sustainable products has increased along the years, since the choice of products, packaging and production processes have a great impact on the environment. These products are classified by regulatory agencies in different categories, aggregating advantages to the product and increasing the demand by consumers. However, there is no harmonization in guidelines of these certifying agencies and each cosmetic industry formulates their product and packaging in a more rational way, which causes less damage to the environment. Many cosmetic products have in their formulation natural products that perform a specific biological function, but these products should be evaluated on efficacy and toxicological aspects. The aim of this article is to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic cosmetics; Natural cosmetics; Natural products; Sustainability; Cosmetics; Cosmetics.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100017

Comparative study of the stability of free and modified papain incorporated in topical formulations
Provedor de dados: 60
Autores: Pinto,Claudinéia Aparecida Sales de Oliveira; Lopes,Patricia Santos; Sarruf,Fernanda Daud; Polakiewicz,Bronislaw; Kaneko,Telma Mary; Baby,Andre Rolim; Velasco,Maria Valéria Robles.
Papain is an enzyme used in topical formulations as a proteolytic debriding agent for the treatment of open, extensive wounds and burnings. It is also employed as an enhancer for cutaneous permeation of active compounds, chemical peeling and as a progressive depilatory agent. The stability of formulations containing enzymes is not easy. In this research, papain was modified with polyethylene glycol in order to increase the stability of the formulations. The comparative Normal Stability Testing of the topical formulations containing unmodified and modified papain showed that the modified variety presented with a differentiated profile under the adopted temperature conditions (5.0 ± 1.0 °C; 22.0 ± 2.0 °C; 40.0 ± 2.0 °C). The most suitable condition for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Papain/use in topical formulations; Papain/normal stability test; Poliethylene glycol/use; Topical formulations/development; Papain/enzimatic activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400012

The plasma Tumor Necrosis Factor-α (TNF-α) does not have any correlation with disease activity in rheumatoid arthritis patients treated with disease modifying anti-rheumatic drugs (DMARDs)
Provedor de dados: 60
Autores: Samimi,Zahra; Kardideh,Bahareh; Chalabi,Maryam; Zafari,Parisa; Taghadosi,Mahdi.
We performed this study to measure the Tumor Necrosis Factor-alpha (TNF-α) plasma level and to survey its correlation with disease activity in the newly diagnosed Rheumatoid Arthritis (RA) patients and those who were under treatment with the combination of Disease-Modifying Anti-Rheumatic Drug (DMARD) plus Prednisolone (PSL).We enrolled 30 newly diagnosed RA patients who received no treatment regarding their disease, 30 patients under treatment with the combination of Methotrexate (MTX) + Hydroxychloroquine (HCQ) + PSL and 30 healthy subjects in this case-control study from September 2017 to December 2017. The level of plasma TNF-α was measured by enzyme-linked immunosorbent assay (ELISA) in each group. For assessment of disease severity, we used Disease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; TNF-α; DAS-28; DMARD.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100601

Stability-indicating liquid chromatographic and UV spectrophotometric methods for the quantification of ciprofibrate in capsules and tablets
Provedor de dados: 60
Autores: Hellwig,Fernanda Macke; Reis,Rafael Henrique Dias; Barbosa,Suzana Del Rosso; Malesuik,Marcelo Donadel.
This study describes the development and evaluation of stability-indicating liquid chromatographic (LC) and UV spectrophotometric methods for the quantification of ciprofibrate (CPF) in tablets and capsules. Isocratic LC separation was achieved on a RP18 column using a mobile phase of o-phosphoric acid (0.1% v/v), adjusted to pH 3.0 with triethylamine (10% v/v) and acetonitrile (35:65 v/v), with a flow rate of 1.0 mL min-1. Detection was achieved with a photodiode array detector at 233 nm. For the spectrophotometric analysis, ethanol and water were used as the solvent and a wavelength of 233 nm was selected for the detection. The methods were validated according to International Conference on Harmonization (ICH) guidelines for validating analytical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciprofibrate/tablets/quality control; Ciprofibrate/capsules/quality control; UV Spectrophotometry/quantitative analysis; Liquid chromatography/stability study; Medicines/quality control.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100221

Evaluation of the effectiveness of an Internet-based continuing education program on pharmacy-based minor ailment management: a randomized controlled clinical trial
Provedor de dados: 60
Autores: Obreli-Neto,Paulo Roque; Guidoni,Camilo Molino; Baldoni,André de Oliveira; Marusic,Srecko; Castedalli,Gilson; Lahoud,Yesmin Marie; Soares,Christian Franco; Pereira,Leonardo Régis Leira.
ABSTRACT The aim of this work was to evaluate the effectiveness of an internet-based continuing education (CE) program on pharmacy-based minor ailment schemes (PMASs). A controlled randomized clinical trial was conducted in community pharmacies in Brazil. Community pharmacists (CPs) were enrolled in two groups: intervention (n = 61) and control (n = 60). CPs who were enrolled to the intervention group participated in an Internet-based CE program. CPs in the control group received no educational intervention. We evaluated participant perception, learning outcomes, and practice performance. Learner satisfaction with the CE program was high for every point evaluated (mean ± standard deviation = 4.2 ± 0.4). Posttest learner outcome scores and practice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Continuing education; Internet-based continuing education; Drugs/nonprescription; Community pharmacy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100003

Diagnosis of the availability and use of drug information sources in drugstores and pharmacies in southern Brazil
Provedor de dados: 60
Autores: Hennigen,Fabiana Wahl; Fischer,Maria Isabel; Camargo,Aline Lins; Heineck,Isabela.
This research has aimed to estimate the use of drug information sources by pharmacists in drugstores and pharmacies in southern Brazil. It consisted of sending a questionnaire through regular mail, contacting the pharmacist via phone and visiting the drugstores. Four hundred and eight (68.6%) of the 595 enrolled establishments answered the questionnaire. The information at pharmacies and drugstores is searched mainly to orient the patient. At drugstores the professionals have an average of 2.3 books, whereas at pharmacies they rely on 6.1. In a pharmacy, the chance to find more than five books is 27 times higher than in a drugstore. The more often available books are pharmaceutical specialties compendiums. There is access to Internet in 87.5% of pharmacies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical assistance; Drugs/information availability; Pharmaceutical practice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200014

Evaluation of the pharmaceutical assistance structure in Maranhão State, Brazil
Provedor de dados: 60
Autores: Maximino,Flávio Donalwan Sá; Brito,Maria Helena Seabra Soares de; Castilho,Selma Rodrigues de; Cordeiro,Benedito Carlos.
Assessment instruments can measure the effectiveness of health policy organizations. This research is a descriptive diagnostic study the Pharmaceutical Assistance structure in Maranhão State, Brazil. The methodology consisted of obtaining secondary data from institutions related to the Pharmaceutical Assistance in the State. Structure indicators were calculated using methodology adapted from the World Health Organization (WHO), and pharmacists perception about the structure of the Pharmaceutical Assistance was analysed through questionnaires. There are 3,003 pharmacists and 3,410 healthcare establishments registered in the Regional Pharmacy Council CRF-MA, particularly in the capital area. Two main problems were identified by pharmacists: the “ineffective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical assistance; National medicament policy; Process assessment; Brazil Health System.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100545

Compliance with treatment: related-issues and insights for pharmacist intervention
Provedor de dados: 60
Autores: Silva,Nilcéia Lopes da; Ribeiro,Eliane; Navarro,João Luis; Zanini,Antonio Carlos.
Low patient compliance with pharmacotherapy remains one of the greatest challenges for success of treatments, especially in chronic diseases, since it can negatively influence treatment effectiveness and patient quality of life, increase health expenses and decrease productivity of the patient. Compliance is an important but complex issue in clinical practice. Its complexity begins with the difficulty in adopting terminology that can express its exact meaning. Moreover, many methods to evaluate compliance have been established but no consensus exists on which method should be considered the gold standard. Additionally, socioeconomic, disease and therapy-related factors, healthcare team and system related-factors and patient-related factors can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacotherapy/compliance with treatment; Medicines/use; Chronic diseases/therapy; Pharmacists/interventions.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100002

Tetramethylpyrazine protects Schwann cells from ischemia-like injury and increases cell survival in cold ischemic rat nerves
Provedor de dados: 60
Autores: Yang,Ming-Ming; Huang,Wei; Jiang,Dian-Ming.
Tetramethylpyrazine (TMP), a major active ingredient of Ligusticum wallichi Franchat extract (a Chinese herb), exhibits neuroprotective properties in ischemia. In this study, we assessed its protective effects on Schwann cells (SCs) by culturing them in the presence of oxygen glucose deprivation (OGD) conditions and measuring cell survival in cold ischemic rat nerves. In the OGD-induced ischemic injury model of SCs, we demonstrated that TMP treatment not only reduced OGD-induced cell viability losses, cell death, and apoptosis of SCs in a dose-dependent manner, and inhibited LDH release, but also suppressed OGD-induced downregulation of Bcl-2 and upregulation of Bax and caspase-3, as well as inhibited the consequent activation of caspase-3. In the cold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetramethylpyrazine; Schwann cell; Oxygen glucose/deprivation; Peripheral nerve; Ischemia/experimental study; University of Wisconsin solution.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100127

Tetracycline: production, waste treatment and environmental impact assessment
Provedor de dados: 60
Autores: Borghi,Alexandre Augusto; Palma,Mauri Sergio Alves.
The frequent occurrence of pharmaceuticals in the aquatic environment requires an assessment of their environmental impact and their negative effects in humans. Among the drugs with high harmful potential to the environment are the antibiotics that reach the environment not only, as may be expected, through the effluents from chemical and pharmaceutical industries, but mainly through the sewage and livestock; because around 25 to 75% of the ingested drugs are excreted in unchanged form after the passage through the Gastro-Intestinal Tract. Tetracycline has high world consumption, representing a human consumption of about 23 kg/day in Brazil in 2007. At the moment, researches are being made to develop new tetracycline that incorporate heavy metals (Hg, Cd,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetracyclines/production; Tetracyclines/waste/tratament; Tetracyclines/waste/environmental impact; Environmental contamination; Domestic sewage/treatment; Industrial wastewater/treatment; Advanced oxidation processes/wastewater treatment.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100003

Antioxidant capacity of extracts and isolated compounds from Stryphnodendron obovatum Benth
Provedor de dados: 60
Autores: Zocoler,Analice Martins Daleffi; Sanches,Andréia Cristina Conegero; Albrecht,Ingrid; Mello,João Carlos Palazzo de.
The extract from stem bark of Stryphnodendron obovatum Benth. was chromatographed on a Sephadex® LH-20 column, and yielded nine compounds: gallic acid (GA), p-hydroxybenzoic acid (PHB), gallocatechin (GC), epigallocatechin (EPG), 4'-O-methylgallocatechin (MGC), epigallocatechin-(4β→8)-epigallocatechin (EPEP), epigallocatechin-(4β→8)-gallocatechin (EPGC), robinetinidol-(4α→8)-gallocatechin (ROGC) and robinetinidol-(4β→8)-epigallocatechin (ROEP). Evaluation of the antioxidant capacity in vitro by the methods of DPPH free radical (IC50; μg/mL) and reduction of the phosphomolybdenum complex (RAC) gave the following results, respectively: crude extract 4.52 and 0.8242; ethyl-acetate fraction 4.04 and 0.9537; aqueous fraction 5.58 and 0.9275. The crude extract...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stryphnodendron obovatum; Antioxidant capacity; Condensed tannins; DPPH free radical; Phosphomolybdenum complex.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300009

Determination of moisture content in lyophilized mannitol through intact glass vials using NIR micro-spectrometers
Provedor de dados: 60
Autores: Muzzio,Cristian Rodrigo; Dini,Nicolás Gabriel; Simionato,Laura Daniela.
Determination of moisture content in lyophilized solids is fundamental to predict quality and stability of freeze-dried products, but conventional methods are time-consuming, invasive and destructive. The aim of this study was to develop and optimize a fast, inexpensive, noninvasive and nondestructive method for determination of moisture content in lyophilized mannitol, based on an NIR micro-spectrometer instead of a conventional NIR spectrometer. Measurements of lyophilized mannitol were performed through the bottom of rotating glass vials by means of a reflectance probe. The root mean standard error of prediction (RMSEP) and the correlation coefficient (R²pred), yielded by the pre-treatments and calibration method proposed, was 0.233% (w/w) and 0.994,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Near-infrared spectroscopy; Lyophilization; Multivariate analysis; Moisture content; Mannitol; Micro-spectrometer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200010

Pharmacokinetics of oleracimine in rats by ultra-high-performance liquid chromatography
Provedor de dados: 60
Autores: Qu,Gonglin; Xu,Liang; Zhang,Wenjie; Ying,Xixiang.
The novel alkaloid, oleracimine, presented remarkable anti-inflammatory bioactivity, and therefore, its pharmacokinetics was investigated in rat plasma after intravenous and oral administration by using a rapid ultra-high-performance liquid chromatography (UHPLC) method with UV detection at 270 nm. The analysis was performed on a shim-pack ODS column (75 mm×2 mm, 1.6 µm particle size, Shimadzu, Japan) column using isocratic elution with a mobile phase consisting of methanol-water (62:38, v/v) within 3 min. The results indicated that oleracimine was rapidly distributed with Tmax for 11.7 min after oral administration, which presented the double-peak phenomenon in the pharmacokinetic profile with a higher oral absolute bioavailability of 55.1% ± 7.83%.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Portulaca oleracea L./Oleracimine; Ultra-High-Performance Liquid Chromatography/Rat plasma; Pharmacokinetics.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400611

Pharmaceutical properties of 'sucupira' (Pterodon spp.)
Provedor de dados: 60
Autores: Hansen,Daiane; Haraguchi,Mitsue; Alonso,Antonio.
The plant of the genus Pterodon (Fabaceae, Leguminosae), commonly known as 'sucupira' or 'faveira', are disseminated throughout the central region of Brazil and has frequently been used in popular medicine for its anti-rheumatic, analgesic, and anti-inflammatory properties. In recent years, interest in these plants has increased considerably. The biological effects of different phytoextracts and pure metabolites have been investigated in several experimental models in vivo and in vitro. The literature describes flavonoids, triterpene and steroids, while one paper presented studies with proteins isolated from the genus. This review provides an overview of phytochemical and pharmacological research in Pterodon, showing the main chemical compounds studied to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sucupira; Pterodon/phytochemistry; Pterodon/pharmacognosy; Diterpenes/biological activity; Isoflavones/biological activity; Medicinal plants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400002

Development and validation of a stability indicating HPLC method to determine diltiazem hydrochloride in tablets and compounded capsules
Provedor de dados: 60
Autores: Souza,Mateus Araújo Castro e; Pereira,Carlos Eduardo de Oliveira; Nogueira,Fernando Henrique Andrade; Pianetti,Gerson Antônio.
ABSTRACT A stability indicating HPLC method to determine diltiazem hydrochloride (DTZ) in tablets and compounded capsules was developed and validated according to Brazilian and the International Conference on Harmonization (ICH) guidelines. The separation was carried out on a Purospher Star® C18 (150 x 4.6 mm i.d., 5 µm particle size, Merck Millipore) analytical column. The mobile phase consisted of a 0.05% (v/v) trifluoroacetic acid aqueous solution and a 0.05% trifluoroacetic acid methanolic solution (44:56, v/v). The flow rate was 1.0 mL.min-1 with a run time of 14 minutes. The detection of DTZ and degradation products (DP) was performed at 240 nm, using a diode array detector. The method proved to be linear, precise, accurate, selective, and robust,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diltiazem hydrochloride/compounded capsules; Diltiazem hydrochloride/tablets; High performance liquid chromatography /method validation; Stability indicating method.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300602

Cross-cultural adaptation of Hypertension Knowledge Test into European Portuguese
Provedor de dados: 60
Autores: Cabral,Ana C.; Moura-Ramos,Mariana; Castel-Branco,Margarida; Caramona,Margarida; Fernandez-Llimos,Fernando; Figueiredo,Isabel V.
ABSTRACT We aim to validate a European-Portuguese version of the Hypertension Knowledge Test (HKT) questionnaire and examine its factorial structure with a confirmatory factor analysis (CFA). A process of translation and back-translation was performed. A cross-sectional study was developed in which all adult patients taking at least one antihypertensive drug were invited to participate. Data on personal and family history were collected, and the HKT, Strelec, and the Batalla questionnaires were administered. We enrolled 304 patients with a mean age of 68.12±10.83 years. The mean score of HKT was 15.33±2.79. CFA indicated that the construct being tested was unidimensional, and Cronbach’s alpha (α=0.65) showed that the instrument had an acceptable internal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Health Knowledge/attitudes/practice; Hypertension/prevention and control; Psychometrics; Surveys and questionnaires/standards; Portugal.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400608

Sucrose hydrolysis by invertase using a membrane reactor: effect of membrane cut-off on enzyme performance
Provedor de dados: 60
Autores: Addezio,Francesco Di; Yoriyaz,Ester Junko; Cantarella,Maria; Vitolo,Michele.
Sucrose hydrolysis by invertase [EC.3.2.1.26] produces inverted sugar syrup, an ingredient mainly used in the food industry. To properly catalyze hydrolysis, the enzyme should be reused after this reaction. It is advisable to maintain constant activity over a considerable period. Thus, sucrose hydrolysis was performed in a membrane bioreactor - a continuously stirred tank reactor coupled with an ultrafiltration membrane (UFM) which provides good diffusion and high activity per unit volume. Molecular weight cut-off for soluble invertase UFMs was up to 100kDa. This study focused on the role of UFM invertase cut-off as it is the main element in the process. We demonstrated that both the cut-off and chemical nature of the UFM affected specific invertase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Invertase; Membrane Reactor; Sucrose/hydrolysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200257

Evaluation of the antinociceptive and anti-inflammatory effects of the acetone extract from Anacardium occidentale L
Provedor de dados: 60
Autores: Vanderlinde,Frederico Argollo; Landim,Higor Frutuoso; Costa,Elson Alves; Galdino,Pablinny Moreira; Maciel,Maria Aparecida Medeiros; Anjos,Gineide Conceição dos; Malvar,David do Carmo; Côrtes,Wellington da Silva; Rocha,Fábio Fagundes da.
The stem bark of Anacardium occidentale L. (Anacardiaceae), commonly called cashew, is used in Brazilian traditional medicine for the treatment of gastric and inflammatory disorders. The present study was carried out to investigate the in vivo anti-inflammatory activities of the acetone extract (AE) of the stem bark of A. occidentale. We evaluated the pharmacological activities of this plant material through the analgesic, antiedematogenic and chemotaxic inhibitory effects produced by the AE. The oral administration (p.o.) of mice with the AE (0.1, 0.3 and 1.0 g/kg) or positive control indomethacin (10 mg/kg) inhibited acetic acid-induced writhing by 18.9, 35.9, 62.9 and 68.9%, respectively (ID50% = 530 mg/kg). The highest dose of the AE was able to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anacardium occidentale L./analgesic activity; Anacardium occidentale L./anti-inflammatory activity; Anacardium occidentale L./antiedematogenic activity; Anacardiaceae; Cashew.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300008

Anti-inflammatory activity and identification of the Verbena litoralis Kunth crude extract constituents
Provedor de dados: 60
Autores: Lima,Rachel de; Brondani,Juliana Calil; Dornelles,Rafaela Castro; Lhamas,Cibele Lima; Faccin,Henrique; Silva,Carine Viana; Dalmora,Sérgio Luiz; Manfron,Melânia Palermo.
Verbena litoralis is a plant popularly known as “gervãozinho-do-campo” in Portuguese. It is traditionally used for stomach, liver and gallbladder problems, and as an anti-inflammatory and anthelmintic. The goal of this study was to determine the chemical composition of the crude extract obtained from the aerial parts of V. litoralis by Ultra High Performance Liquid chromatography coupled with Mass Spectrometry in Tandem (UHPLC-MS/MS); assess the anti-inflammatory activity of ethyl acetate fraction and of crude extract; and verify liver, kidney and pancreas damage. In this study, the chemical composition of the extract was identified via UHPLC/MS/MS, assessing the anti-inflammatory activity of the crude extract and the acetate fraction in an induction model...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Verbena litoralis; UHPLC/MS; Flavonoids; Anti-inflammatory.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100540

Genotoxic and chromatographic analyses of aqueous extracts of Peltodon longipes Kunth ex Benth. (hortelã-do-campo)
Provedor de dados: 60
Autores: Kuhn,Andrielle Wouters; Tedesco,Marília; Boligon,Aline Augusti; Frescura,Viviane Dal-Souto; Athayde,Margareth Linde; Tedesco,Solange Bosio.
Peltodon longipes is used as a stimulant and emmenagogue. The objective of this study was to perform genotoxic and chromatographic analyses of the extracts of two samples of P. longipes, collected from the cities of Santa Maria and Tupanciretã, RS, Brazil. The Allium cepa assay was used to analyze genotoxicity while high-performance liquid chromatography was employed to determine phenolic compounds. The genotoxicity experiment consisted of nine groups each comprising four A. cepa bulbs. Bulb roots were developed in distilled water and then transferred for the treatments, for 24 hours, and the negative control remained in water. The treatments were: aqueous extracts at concentrations of 5 and 15 g L-1 for each sample, plus four groups treated with 1%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P. longipes/antiproliferative effect; P. longipes/genotoxic potential; P. longipes/chromatographic analyses; Allium cepa test/genotoxicity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300533

Preparation, characterization, and antiproliferative activities of biotin-decorated docetaxel-loaded bovine serum albumin nanoparticles
Provedor de dados: 60
Autores: Cheng,Kai; Sun,Shaoping; Gong,Xianfeng.
ABSTRACT The aim of the present study was to characterize biotin-decorated docetaxel-loaded bovine serum albumin nanoparticles (DTX-BIO-BSA-NPs) and evaluate their antiproliferative activity in vitro. The particle size of prepared DTX-BIO-BSA-NPs was found to be always lower than 200 nm, with sizes of 166.9, 160.3, 159.0, 176.1 and 184.8 nm and the zeta potential was -29.51, -28.54, -36.54, -36.08 and -27.56 mV after redissolution with water for 0, 1, 2, 4 and 8 hours, respectively. The polydispersity index (PDI) was stable in the range of 0.170 - 0.178. In the in vitro drug-release study, the DTX-BIO-BSA-NPs targeted a human breast cancer cell line MCF-7 effectively. The x-ray diffraction spectrum and DSC curve of DTX-BIO-BSA-NPs suggested that docetaxel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albumin/nanoparticles/characterization; Docetaxel; Antiproliferative activities; Biotin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200617

Pharmacokinetics and safety of repirinast tablets in healthy Chinese subjects
Provedor de dados: 60
Autores: Lv,Cheng-zhe; Huang,Ming; Zhang,Quan-ying; Zong,Shun-lin; Wang,Meng.
ABSTRACT Repirinast is a new, synthetic, disodium cromoglycate-like antiallergic agent for oral administration in humans. This study evaluated the safety, tolerability and pharmacokinetics of repirinast tablets in healthy Chinese volunteers. This was a phase I, open-label, randomized, single- and multiple-dose study. Subjects were assigned to receive a single dose of repirinast tablet at either 150, 300, or 450 mg, or multiple doses of 150 mg twice daily for 5 days. Plasma samples were analyzed with LC-MS/MS. Pharmacokinetic parameters of active metabolite MY-1250 (deesterified repirinast) were calculated using non-compartmental analysis with WinNonlin software. Statistical analysis was performed using SPSS software. All adverse events (AEs) were mild and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Repirinast/safety/pharmacokinetics; Single dose; Multiple dose; China.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200625

Thermoanalytical studies of carbamazepine: hydration/dehydration, thermal decomposition, and solid phase transitions
Provedor de dados: 60
Autores: Pinto,Mônia Aparecida Lemos; Ambrozini,Beatriz; Ferreira,Ana Paula Garcia; Cavalheiro,Éder Tadeu Gomes.
Carbamazepine (CBZ), a widely used anticonvulsant drug, can crystallize and exhibits four polymorphic forms and one dihydrate. Anhydrous CBZ can spontaneously absorb water and convert to the hydrate form whose different crystallinity leads to lower biological activity. The present study was concerned to the possibility of recovering the hydrated form by heating. The thermal behavior of spontaneously hydrated carbamazepine was investigated by TG/DTG-DTA and DSC in dynamic atmospheres of air and nitrogen, which revealed that the spontaneous hydration of this pharmaceutical resulted in a Form III hydrate with 1.5 water molecules. After dehydration, this anhydrous Form III converted to Form I, which melted and decomposed in a single event, releasing isocyanic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbamazepine/thermal analysis; Polymorphism; Hydrate; Dehydration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400877

Analysis of antimicrobials' consumption profile in a University Hospital of Western Paraná, Brazil
Provedor de dados: 60
Autores: Caldeira,Luciane de Fátima; Burattini,Marcelo Nascimento.
The objective of this study was to analyze the variation in antimicrobials' consumption and the costs related to their use at a University Hospital between 1999 and 2004. The annual consumption of nine antimicrobials, expressed in DDD/100 patients-day, and the direct costs with their acquisition were evaluated. Analysis of variance and regression techniques were used to compare data, considering a significance level of 5%. The most consumed antimicrobials were amikacin and ceftriaxone. In general, antimicrobials consumption, expressed in DDD/100 patients-day, increased from 9.21 in 1999 to 25.08 in 2004 (p&lt;0.0001). When analyzing antimicrobial consumption as related to specific hospital units, the ICU showed the highest consumption followed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/Defined Daily Doses; Medicines/utilization; Antimicrobials/consumption; Pharmacoepidemiology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200015

Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook
Provedor de dados: 60
Autores: Emir,Ahmet; Emir,Ceren; Bozkurt,Buket; Onur,Mustafa Ali; Somer,Nehir Unver; Kaya,Gulen Irem.
ABSTRACT In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaloids; Amaryllidaceae; Plant extracts; High Performance Liquid Chromatography/method validation; High Perfomance Liquid Chromatography/quantitative analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100603

Influence of lipids and proteins amounts and pH values on the inhibitory effects of Origanum vulgare L. essential oil against Escherichia coli and Salmonella Typhimurium
Provedor de dados: 60
Autores: Medeiros,Andrea Sulamita de Jesus; Carvalho,Rhayane Idalyne; Conceição,Maria Lúcia da; Magnani,Marciane; Souza,Evandro Leite de.
Origanum vulgare L. (OVEO) essential oil has been considered a candidate antimicrobial for use in food conservation systems. However, studies on the influence of concomitant variations of different food components or physicochemical parameters on the antibacterial properties of OVEO are scarce. This study assessed the influence of concomitant variations in amounts of proteins - PTN (4.0, 6.0 or 8.0 g/100 mL) and lipids - LIP (3.75, 5.0 or 6.25 g/100 mL) and pH values (5.0, 5.5 or 6.0) in cultivation medium on the inhibitory effects of OVEO against Escherichia coli (EC) and Salmonella Typhimurium (ST). Lowest minimum inhibitory concentration values of OVEO against EC and ST were observed in media with the highest LIP amounts regardless the PTN amount and pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oregano; Antibacterial effects; Influential factors; Foodborne pathogens.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100622

Morpho-anatomical characters of the leaf and stem of Mandevilla coccinea (Hook. et Arn.) Woodson, Apocynaceae
Provedor de dados: 60
Autores: Duarte,Márcia do Rocio; Larrosa,Carina Rau Rivas.
Mandevilla coccinea (Hook. et Arn.) Woodson, Apocynaceae is a herb native to South America employed in folk medicine as an analgesic, anti-inflammatory and to inhibit snake venom effects. This work was carried out to study the morpho-anatomical characters of the leaf and stem in order to advance knowledge on this medicinal plant and on pharmacognostic quality control. The plant material was fixed and prepared according to light and scanning electron microtechniques. Its leaves are simple, alternate and ovate-obovate. The epidermis is uniseriate and coated with a thick and striate cuticle. The stomata are paracytic and occur on both foliar surfaces. The dorsiventral-like mesophyll has a sub-epidermal parenchymatic layer containing phenolic substances and is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mandevilla coccinea (Hook. et Arn.) Woodson/pharmacognosy; Mandevilla coccinea (Hook. et Arn.) Woodson/morpho-anatomical characters; Apocynaceae/pharmacognosy; Medicinal plants/pharmacognostic quality control; Phenolic compounds.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100017

Effects of treatment with sitagliptin on hepatotoxicity induced by acetaminophen in mice
Provedor de dados: 60
Autores: Hewedy,Wafaa Ahmed.
Up to date, the management of hepatotoxicity induced by a suicidal or unintentional overdose of acetaminophen (APAP) remains a therapeutic challenge. The present study aimed to elucidate the potential effect of sitagliptin, a DPP-4 inhibitor, to ameliorate the acute injurious effects of acetaminophen on the liver. APAP toxicity was induced in mice by an intraperitoneal injection of APAP (400 mg/kg). The effect of treatment with sitagliptin, initiated 5 days prior to APAP injection, was evaluated. Serum indices of hepatotoxicity, oxidative stress markers in liver tissues, serum IL-1β, and TNF-α in addition to hepatic- NF-E2-related factor-2 (Nrf2) were determined. Our results showed that APAP induced marked hepatic injury as evidenced by an increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetaminophen; Dipeptidyl peptidase-4 inhibitors; Hepatotoxicity; Nrf2; Sitagliptin.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100610

Chalcones and N-acylhydrazones: direct analogues? Exploratory data analysis applied to potential novel antileishmanial agents
Provedor de dados: 60
Autores: Rando,Daniela Gonçalves; Giarolla,Jeanine; Pasqualoto,Kerly Fernanda Mesquita; Ferreira,Elizabeth Igne.
Leishmaniasis is an important health and social problem for which there is limited effective therapy. Chalcones and N-acylhydrazones have been studied as promising antileishmanial agents in enzymatic inhibition and in vitro assays. Since these chemical classes of compounds also resemble each other structurally, it would be useful to investigate whether they share direct analogy. Exploratory data analysis was applied to a library of chalcones and nitrated N-acylhydrazones assayed against Leishmania donovani to investigate their similarity. Under the conditions applied in the present study, the two classes did not present functional or structural analogy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chalcones; N-acylhydrazones; Antileishmanial agents; Leishmaniasis; Chemometrics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200019

Association of glycemic control with progression of diabetic retinopathy in type 2 diabetes mellitus patients in Malaysia
Provedor de dados: 60
Autores: Huri,Hasniza Zaman; Huey,Chua Chew; Mustafa,Norasyikin; Mohamad,Nor Fadhilah; Kamalden,Tengku Ain.
Abstract The risk of having microvascular complication is high among Type 2 Diabetes Mellitus (T2DM) patients. However, factors associated with the glycemic control and progression of diabetic retinopathy (DR) in T2DM patients is limited. This study aims to determine association between anti-diabetic agents, glycemic control and progression of diabetic retinopathy in a Malaysian population. A retrospective study conducted in a tertiary teaching hospital in Malaysia, from January 2009 until March 2014. This study enrolled 104 patients aged 40-84 years, with a mean age 63.12 ± 9.18 years. patients had non-proliferative diabetic retinopathy (NPDR, 77%) and 35% had proliferative diabetic retinopathy (PDR). Diabetic macula edema (DME) was present in 20% of NPDR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 Diabetes Mellitus; Anti-diabetic agents; Microvascular complication; Diabetic macula edema; Diabetic retinopathy; Malaysia.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200608

The Liquisolid technique: an overview
Provedor de dados: 60
Autores: Burra,Shashidher; Yamsani,Madhusudhanrao; Vobalaboina,Venkateswarlu.
A novel "Powder Solution Technology" involves absorption and adsorption efficiency which makes use of liquid medications, drug suspensions admixed with suitable carriers, coating materials and formulated into free flowing, dry looking, non adherent and compressible powder forms. Based upon a new mathematical model expression, improved flow characteristics and hardness of the formulation has been achieved by changing the proportion of Avicel ® PH 200 and Aerosil ® PH 200 from 50:1 ratio to 5:1 and in which the drug is dispersed in an almost molecularly state. Due to their significantly improved wetting properties a greater drug surface area is exposed to the dissolution media, resulting in an increased dissolution rate and bio availability. By using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liquisolid; Solubility; Bioavailability; Flow properties; Load factor; Powder solution technology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300005

Production and characterization of alginate-starch-chitosan microparticles containing stigmasterol through the external ionic gelation technique
Provedor de dados: 60
Autores: Fujiwara,Gislene Mari; Campos,Ranieri; Costa,Camila Klocker; Dias,Josiane de Fátima Gaspari; Miguel,Obdulio Gomes; Miguel,Marilis Dallarmi; Marques,Francisco de Assis; Zanin,Sandra Maria Warumby.
Stigmasterol - a plant sterol with several pharmacological activities - is susceptible to oxidation when exposed to air, a process enhanced by heat and humidity. In this context, microencapsulation is a way of preventing oxidation, allowing stigmasterol to be incorporated into various pharmaceutical forms while increasing its absorption. Microparticles were obtained using a blend of polymers of sodium alginate, starch and chitosan as the coating material through a one-stage process using the external gelation technique. Resultant microparticles were spherical, averaging 1.4 mm in size. Encapsulation efficiency was 90.42% and method yield 94.87%. The amount of stigmasterol in the oil recovered from microparticles was 9.97 mg/g. This technique proved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stigmasterol/microencapsulation; Microencapsulation; Sodium alginate/drugs coating; Chitosan/drugs coating; Starch/drugs coating; Natural polymers/drugs coating.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300015

Anti-inflammatory, analgesic, and immunostimulatory effects of Luehea divaricata Mart. &amp;amp; Zucc. (Malvaceae) bark
Provedor de dados: 60
Autores: Rosa,Roseane Leandra da; Nardi,Geisson Marcos; Januário,Adriana Graziele de Farias; Boçois,Renata; Bagatini,Katiane Paula; Bonatto,Sandro José Ribeiro; Pinto,Andrea de Oliveira; Ferreira,João Ronaldo Notargiacomo; Mariano,Luisa Nathália Bolda; Niero,Rivaldo; Iagher,Fabíola.
Luehea divaricata (Malvaceae) is a plant widely used for treatment of various inflammatory and infectious conditions; however few reports discuss its biological properties. The aim of this study was to evaluate the anti-inflammatory and analgesic effects as well as the macrophage activity in mice treated with the hydroalcoholic crude extract of L. divaricata(CLD). Thin layer chromatography revealed presence of epicathequin, stigmasterol, lupeol and α,β-amyrin in the extract. To evaluate the anti-inflammatory and analgesic activities, animals were subjected to paw edema induced by carrageenan test, writhing, formalin and capsaicin tests. Immunomodulatory activity was evaluated by adhesion and phagocytic capacity, lysosomal volume, and reactive oxygen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Luehea divaricata Mart. &amp;amp; Zucc./pharmacognosy Luehea divaricata Mart. &amp;amp; Zucc./hydroalcoholic crude extract/properties Anti-inflammatories Analgesics Reactive oxygen species.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300599

Antifungal effect of sesame medicinal herb on Candida Species: original study and mini-review
Provedor de dados: 60
Autores: Lavaee,Fatemeh; Moshaverinia,Maryam; MalekHosseini,Seyed Ali; Jamshidzade,Akram; Zarei,Maryam; Jafarian,Hadis; Haddadi,Pardis; Badiee,Parisa.
The aim of this study was to evaluate the antifungal susceptibility patterns of three antifungals, methanolic extracts and N-hexane oil of sesame seeds on C. albicans and C. glabrata, isolated from oral cavity of liver transplant recipients. The results were compared with other reports to develop a mini review as well. Candida species were isolated from liver transplant recipients. To evaluate the antifungal activity of sesame seed oil and methanolic extract, fluconazole, caspofungin and nystatin, the corresponding minimum inhibitory concentrations were determined by CLSI M27-A3 standard method. Minimum fungicidal concentration was also evaluated. The most prevalent species was C. albicans, followed by C. glabrata. Findings indicated sensitivity to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida species/ antifungal susceptibility; Sesame/study; Liver transplant.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100530

Novel Eudragit® -based polymeric nanoparticles for sustained release of simvastatin
Provedor de dados: 60
Autores: Rodrigues,Deborah Fernandes; Couto,Renê Oliveira do; Sinisterra,Rubén Dario; Jensen,Carlos Eduardo de Matos.
This paper reports on the development of nanoparticles aiming at the in vitro controlled release of simvastatin (SVT). The nanoparticles were prepared by the nanoprecipitation method with polymers Eudragit® FS30D (EDGFS) or Eudragit® NE30D (EDGNE). EDGFS+SVT nanoparticles showed mean size of 148.8 nm and entrapment efficiency of 76.4%, whereas EDGNE+SVT nanoparticles showed mean size of 105.0 nm and entrapment efficiency of 103.2%. Infrared absorption spectra demonstrated that SVT incorporated into the polymer matrix, especially EDGNE. Similarly, the differential scanning calorimeter (DSC) curve presented no endothermic peak of fusion, indicating that the system is amorphous and the drug is not in the crystalline state. The maintenance of SVT in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simvastatin; Nanoparticles; Poly-methylmethacrylate; Dissolution; Drug delivery systems.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100591

Preparation and characterization of liposomes loaded with silver nanoparticles obtained by green synthesis
Provedor de dados: 60
Autores: Espinoza,Joel Toribio; Novak,Robson Schimandeiro; Magalhães,Cássia Gonçalves; Budel,Jane Manfron; Justus,Barbara; Gonçalves,Melissa Marques; Boscardin,Patricia Mathias Döll; Farago,Paulo Vitor; Paula,Josiane de Fátima Padiha De.
The objective of this work was to develop and characterize liposomes loaded with silver nanoparticles (LAgNPs) to show improvement in stability characteristics. AgNPs were prepared by the green synthesis method with Aloe vera gel extract and exposure to sunlight. Liposomes were prepared by the modified reverse phase method. Particle size, polydispersity index, zeta potential, as well as the scanning electron microscopy (SEM) morphological aspects of AgNPs and LAgNPs were evaluated. In addition, was used flame atomic absorption spectroscopy to determine the amount of AgNP that was encapsulated in liposomes. The AgNPs presented as amorphous and polydisperse structures, with a mean diameter of 278.46 nm and zeta potential of -18.3 mV. LAgNPs had a mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver nanoparticles; Aloe vera; Sunlight; Liposomes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100625

Total polyphenols from Syzygium cumini (L.) Skeels fruit extract
Provedor de dados: 60
Autores: Migliato,Ketylin Fernanda; Carvalho,Elisângela Simões de; Sacramento,Luis Vitor Silva do; Mello,João Carlos Palazzo de; Baby,André Rolim; Velasco,Maria Valéria Robles; Salgado,Hérida Regina Nunes.
A precise, accurate and low cost spectrophotometric method was developed and validated for routine determination of total polyphenols, as pyrogallic acid equivalents, from the percolated and lyophilized extract of Syzygium cumini (L.) Skeels fruits. Validation was assessed experimentally and data were rigorously treated by statistical analysis. Analytical parameters were: linearity, interval (range), precision and recovery/accuracy, limit of detection (LOD, μg mL-1) and limit of quantification (LOQ, μg mL-1). The visible spectrophotometric method presented linearity (r² = 0.9979 ± 0.0010) over the concentration range 0.25-7.5 μg mL-1 of standard pyrogallic acid, precision &lt; 2.918171%, recovery/accuracy ranging from 96.228693 to 107.17701%, LOD = 0.21 μg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total polyphenols; Syzygium cumini; Botanical extract; Analytical method validation; Spectrophotometry.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100015

Short-term high-fat diet affects macrophages inflammatory response, early signs of a long-term problem
Provedor de dados: 60
Autores: Santos,Ed Wilson; Oliveira,Dalila Cunha; Hastreiter,Araceli; Silva,Graziela Batista; Beltran,Jackeline Soares de Oliveira; Rogero,Marcelo Macedo; Fock,Ricardo Ambrósio; Borelli,Primavera.
Obesity is a chronic inflammatory disease that affects millions of people worldwide. Most studies observe the effects of a high-fat diet (HFD) in 10-12 weeks. This work investigated the effects induced by a HFD administered for 6 weeks on the nutritional status of mice and some aspects of the inflammatory response in mouse peritoneal macrophages. Male Swiss Webster mice, 2-3 months of age, were fed a control diet or HFD for 6 weeks. After this period, the mice were euthanized, and peritoneal macrophages were collected for immunoassays and assessment of biochemical parameters. A HFD was associated with increased cholesterol, insulin resistance, C-reactive protein (CRP), leptin, and serum resistin levels. Lipopolysaccharide (LPS)- stimulated adipocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-fat diet/study; Mice; Macrophages; Metabolic syndrome; NF-kB.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100548

Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)
Provedor de dados: 60
Autores: Malheiros,Camila Krüger Cardoso; Silva,Joyce Sayonara Barbosa; Hofmann,Tânia Cristiane; Messina,Thiane Martins; Manfredini,Vanusa; Piccoli,Jacqueline da Costa Escobar; Faoro,Débora; Oliveira,Luís Flávio Souza; Machado,Michel Mansur; Farias,Fabiane Moreira.
ABSTRACT The bark tea of Ceiba speciosa, a tropical tree of the Malvaceae family, is used in the Northwestern Region of Rio Grande do Sul state, Brazil, to reduce blood cholesterol levels. However, there are no scientific data on the efficacy and safety of this plant. The aim of the present study was to evaluate the in vitro antioxidant and toxic potential of bark extracts of C. speciosa. We performed a preliminary phytochemical analysis by high-performance liquid chromatography-diode array detection (HPLC-DAD) and evaluated the oxidative damage to proteins and lipids, the radical scavenging effect, and genotoxicity of the lyophilized aqueous extract (LAECs) and the precipitate obtained from the raw ethanol extract (Cs1). The phytochemical proﬁle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ceiba speciose/phytochemistry; Ceiba speciose/antioxidant activity; Phenolic content; Comet assay; DPPH scavenging capacity; Natural medicine; Safety.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200616

Formulation, preparation and in vitro - in vivo evaluation of compression-coated tablets for the colonic-specific release of ketoprofen
Provedor de dados: 60
Autores: Hales,Dana; Dumitrașcu,Dan Lucian; Tomuță,Ioan; Briciu,Corina; Muntean,Dana-Maria; Tefas,Lucia Ruxandra; Iurian,Sonia; Iovanov,Rareș Iuliu; Achim,Marcela; Vlase,Laurian.
ABSTRACT The aim of this study was to formulate and prepare compression-coated tablets for colonic release (CR-tablets), and to evaluate the bioavailability of ketoprofen following the administration of a single dose from mini-tablets with immediate release (IR-tablets) compared to CR-tablets. CR-tablets were prepared based on time-controlled hydroxypropylmethylcellulose K100M inner compression-coating and pH-sensitive Eudragit® L 30D-55 outer film-coating. The clinical bioavailability study consisted of two periods, in which two formulations were administered to 6 volunteers, according to a randomized cross-over design. The apparent cumulative absorption amount of ketoprofen was estimated by plasma profile deconvolution. CR-tablets were able to delay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colonic delivery; Compression-coated tablets; PH-Dependent release; Time-dependent release; In vitro-in vivo comparison.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400609

Comparison of three experimental designs employed in gentamicin microbiological assay through agar diffusion
Provedor de dados: 60
Autores: Lourenço,Felipe Rebello; Pinto,Terezinha de Jesus Andreoli.
Gentamicin is a broad-spectrum antibiotic complex produced by actinomycetes belonging to Micromonospora genus and classified among aminoglycoside antibiotics, used in the treatment of serious infections derived from Gram-negative microorganisms. Alterations of their antimicrobial activity not shown in chemical assays can be evaluated through microbiological assays. The aim of this work was to compare 5 x 1, 2 x 2 and 3 x 1 experimental designs, evaluating validation parameters of specificity, linearity, range, precision, and accuracy for each experimental design in different levels of concentration, presentation, and lots. It consisted of 81 assays (in 3 replicas) of gentamicin microbiological dosage. The concentrations of the solutions used were employed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gentamicin/sulphate; Gentamicin/experimental design; Gentamicin/microbiological dosage; Antibiotics/qualitative analysis; Aminoglycoside antibiotics/antimicrobial activity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300022

70 years of development of Pharmaceutical Sciences: a lot to celebrate!
Provedor de dados: 60
Autores: Aranha,Leila Rangel de Carvalho.
Tipo: Info:eu-repo/semantics/other
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200001

Square-wave voltammetric determination of rutin in pharmaceutical formulations using a carbon composite electrode modified with copper (II) phosphate immobilized in polyester resin
Provedor de dados: 60
Autores: Freitas,Kellen Heloizy Garcia; Fatibello-Filho,Orlando; Mattos,Ivanildo Luiz de.
A carbon composite electrode modified with copper (II) phosphate immobilized in a polyester resin (Cu3(PO4)2-Poly) for the determination of rutin in pharmaceutical samples by square-wave voltammetry is described herein. The modified electrode allows the determination of rutin at a potential (0.20 V vs. Ag/AgCl (3.0 mol L-1 KCl)) lower than that observed at an unmodified electrode. The peak current was found to be linear to the rutin concentration in the range from 9.9 × 10-8 to 2.5 × 10-6 mol L-1, with a detection limit of 1.2×10-8 mol L-1. The response of the electrode was stable, with no variation in baseline levels within several hours of continuous operation. The surface morphology of the modified electrode was characterized by scanning electron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rutin/determination in pharmaceuticals samples; Square-wave voltammetry/determination of rutin; Modified carbon composite electrode/application.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400007

The importance of participating in the Brazilian Association of Pharmaceutical Sciences
Provedor de dados: 60
Autores: Lopes,João Luis Callegari.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100001

Potentially inappropriate medication use in a city of Southeast Brazil
Provedor de dados: 60
Autores: Pinto,Mauro Cunha Xavier; Ferré,Felipe; Pinheiro,Marcos Luciano Pimenta.
Potentially inappropriate medication use by the Diamantina (Minas Gerais State) population was investigated by analyzing medicine consumption, self-medication, polypharmacy and drug interactions of medicines prescribed among those interviewed. Level of knowledge about rational drug use and its relationship to socio-economic variables was also evaluated using a semi-structured questionnaire. This survey was based on stratified sampling of 423 individuals selected randomly. The prevalence of prescription drug consumption was 42.32% (n=179) and cardiovascular drugs were the most prescribed. Drug interactions were found in 45.81% (n=82) of prescriptions and 92.68% (n=76) of these interactions were moderate, with co-administration of cardiovascular drugs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/rational use; Medicines/inappropriate use; Self-medication; Pharmacoepidemiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100009

Application of statistical process control for spotting compliance to good pharmaceutical practice
Provedor de dados: 60
Autores: Eissa,Mostafa Essam; Abid,Ahmedy Mahson.
ABSTRACT For the release of pharmaceutical products into the drug market; most of the pharmaceutical companies depend on acceptance criteria - that are set internally, regulatory and/or pharmacopeially. However, statistical process control monitoring is underestimated in most quality control in cases; although it is important not only for process stability and efficiency assessment but also for compliance with all appropriate pharmaceutical practices such as good manufacturing practice and good laboratory practice, known collectively as GXP. The current work aims to investigate two tablet inspection characteristics monitored during in-process control viz. tablet average weight and hardness. Both properties were assessed during the compression phase of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Statistical process control (SPC); In-process control; GXP; Film coated tablet; Fluoroquinolone; Capability analysis; Pharmaceutical practice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200301

Preparation and characterization of novel, mucoadhesive ofloxacin nanoparticles for ocular drug delivery
Provedor de dados: 60
Autores: Taghe,Shiva; Mirzaeei,Shahla.
The efficacy of conventional ocular formulations is limited by poor corneal retention and permeation, resulting in low ocular bioavailability. Mucoadhesive chitosan (CS)/ tripolyphosphatesodium (TPP) and chitosan (CS)/ tripolyphosphatesodium (TPP)-alginate (ALG) nanoparticles were investigated for the prolonged topical ophthalmic delivery of ofloxacin. A modified ionotropic gelation method was used to produce ofloxacin-loaded nanoreservoir systems. The ofloxacin-loaded CS/TPP and CS/TPP-ALG nanoparticles were characterized for particle size, morphology, zeta potential, encapsulation efficiency, subsequent release and corneal penetration study. The designed nanoparticles have a particle size from 113.8 nm to 509 nm and zeta potential from 16.2 mV to 40.3 mV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocular drug delivery; Nanoparticles; Corneal penetration; Ofloxacin; Chitosan.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100519

Evaluation of antibiotics dispensing profile in Tubarão, Santa Catarina, Brazil
Provedor de dados: 60
Autores: Rauber,Claudia; Feltrin,Micheli Regina; Piovezan,Anna Paula.
Antibiotics are drugs widely used in prophylaxis and treatment of a great number of diseases. However, their use must be carefully controlled as acquisition in pharmacies, often without medical prescription, is elevated. The role of pharmacists in these circumstances is to practice dispensation in a rational manner. Through a structured questionnaire with open and closed questions, this study investigated the profile of antibiotics dispensed in pharmacies of Tubarão, Santa Catarina, Brazil. From the responses, it was observed that 85.0% dispense this class of medicine without medical prescription, mainly for treatment of respiratory (62.8%) and urinary (12.0%) tract disorders. Moreover, pharmacists' guidance was largely focused on posology (66.6%) and drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacy/medicines control; Antibiotics/dispensation profile; Antibiotics/rational use.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400024

Stability-indicating RP-LC method for quantification of fusidic acid in cream
Provedor de dados: 60
Autores: Curbete,Mariane Machado; Salgado,Hérida Regina Nunes.
ABSTRACT Fusidic acid is an antibiotic steroid indicated for the treatment of infections caused by the genus Staphylococcus, including methicillin resistant Staphylococcus aureus strains, and other Gram-positive bacteria. In the present study, a stability-indicating reversed-phase liquid chromatography (RP-LC) method was developed and validated for the determination of fusidic acid in dermatological cream as an alternative to existing methods. Analyses were performed using a C18 column and guard column at room temperature, eluting with an isocratic mobile phase of acetonitrile and water (72:28, v/v), adjusted to pH 3.5 with acetic acid, pumped at a flow rate of 1.0 mL min-1, detection at 210 nm and 20 µL of injection volume. The forced degradation study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusidic acid/stability-indicating/quality control/validation; Chromatography/reversed-phase.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300447

Renal protective effects of vicenin-2 and scolymoside in a mouse model of sepsis
Provedor de dados: 60
Autores: Lee,Bong-Seon; Yang,Sumin; Lee,Changhun; Ku,Sae-Kwang; Bae,Jong-Sup.
This study was initiated to determine whether 2 structurally related flavonoids found in Cyclopia subternata-vicenin-2 (VCN) and scolymoside (SCL)-could modulate renal functional damage in a mouse model of sepsis, and to elucidate the relevant underlying mechanisms. The potential of VCN and SCL treatment to reduce renal damage induced by cecal ligation and puncture (CLP) surgery in mice was measured via assessment of serum creatinine, blood urea nitrogen (BUN), lipid peroxidation, total glutathione, glutathione peroxidase activity, catalase activity, and superoxide dismutase activity. Treatment with either VCN or SCL resulted in elevated plasma levels of BUN and creatinine, and of protein in the urine of mice with CLP-induced renal damage. Moreover, both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vicenin-2; Scolymoside; Sepsis; Antioxidant; Renal injury; Renal toxicity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100578

Inclusion complexes and self-assembled cyclodextrin aggregates for increasing the solubility of benzimidazoles
Provedor de dados: 60
Autores: Rodrigues,Leticia Norma Carpentieri; Tavares,Anna Carollina Moraes; Ferreira,Beatriz Tavares; Reis,Adriana Karla Cardoso Amorim; Katiki,Luciana Morita.
Albendazole and fenbendazole are imidazole derivatives that exhibit broad spectrum activity against parasites, but the low solubility of these drugs considerably reduces their effectiveness. Complexation of albendazole and fenbendazole with cyclodextrins (β-cyclodextrin and hydroxypropyl-β-cyclodextrin) in both water and an aqueous solution of polyvinylpyrrolidone (PVP-k30) was studied to determine if it could increase the solubility and dissolution rate of the drugs. In an aqueous solution, β-cyclodextrin increased the solubility of albendazole from 0.4188 to ~93.47 µg mL-1 (223×), and of fenbendazole from 0.1054 to 45.56 µg mL-1 (432×); hydroxypropyl-β-cyclodextrin, on the other hand, increased solubility to ~443.06 µg mL-1 (1058×) for albendazole and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclodextrins/pharmacokinetics Benzimidazoles/administration &amp;amp; Dosage Inclusion complexes Cyclodextrin aggregates Drug liberation/drug effects Antiparasitic agents/analysis Albendazole/analysis Fenbendazole/analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100551

Development, in vitro biocompatibility, and antitumor efficacy of acetic acid-modified Cordyceps sinensis polysaccharide nanoparticle drug delivery system
Provedor de dados: 60
Autores: Guan,Jiao; Han,Liqin; Shi,Nianqiu; Zhu,Heyun; Wang,Junmin.
Docetaxel-loaded acetic acid conjugated Cordyceps sinensis polysaccharide (DTX-AA-CSP) nanoparticles were prepared through dialysis and their release rates in vitro, particle sizes, zeta potentials, drug loading capacities, and encapsulation efficiencies were characterized for the synthesis of AA-modified CSPs from traditional Chinese medicine Cordyceps sinensis (Berk.) Sacc. Then, the AA-modified CSPs were characterized by 1H-NMR and FT-IR. Furthermore, the biocompatibility of the delivery carrier (AA-CSP nanoparticles) was assessed on human umbilical vein endothelial cells. In vitro antitumor activity studies on DTX-AA-CSP nanoparticles were conducted on the human liver (HepG2) and colon cancer cells (SW480). The DTX-AA-CSP nanoparticles were spherical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Docetaxel; Cordyceps sinensis polysaccharide; Nanoparticles; Antitumor efficacy; Biocompatibility.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100599

Screening of Ketoprofen-Poloxamer and Ketoprofen-Eudragit solid dispersions for improved physicochemical characteristics and dissolution profile
Provedor de dados: 60
Autores: Savardekar,Ravi Yashwant; Sherikar,Amol Shantinath.
The aim of the present study was to enhance the dissolution rate of an NSAID drug Ketoprofen by formulating it into solid dispersions with water soluble carrier Poloxamer 188 and Eudragit S 100. The solid dispersions of Ketoprofen with Poloxamer 188 were prepared at 1:1, 1:1.5 and 1:2 (Ketoprofen: Poloxamer 188) ratio by Solvent evaporation methods. The same concentration ratio was used for the preparation of solid dispersion with Eudragit S 100 by melting/fusion technique. Further, solid dispersions were investigated by solubility, ATR-FTIR, XRD, DSC, surface morphology, in-vitro dissolution and accelerated stability study. Results demonstrated that both Poloxamer 188 and Eudragit S 100 improve solubility of drugs by 8–10 folds. The result of ATR-FTIR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ketoprofen; Solid Dispersion; Poloxamer 188; Eudragit S 100; Accelerated stability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100579

Do Brazilian hospital pharmacists record, document, archive and disseminate their clinical practice?
Provedor de dados: 60
Autores: Néri,Eugenie Desirèe Rabelo; Vasconcelos,Hemerson Bruno da Silva; Romeu,Geysa Aguiar; Fonteles,Marta Maria de França.
An exploratory study was conducted to present the approach of Brazilian hospital pharmacists to registering, documenting, archiving and disseminating clinical practice. The data were collected using an electronic questionnaire (n=348). In fact, 97.41% of pharmacists record their clinical practice, out of which 64.01% (n=217) do in electronic form, mainly in private hospitals (p&lt;0.000), in the central, southern and southeastern regions (p=0.040), and by professionals with 1-5 years of experience (p=0.001). The main software used is non-specific to clinical practice: an electronic spreadsheet (47.93%; n=104) and text editor (13.37%; n=29). The archiving of records is performed by 87.61% (n=297) of professionals, where 80.13% (n=238) do so in physical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacy; Clinical pharmacy services; Pharmacists/study; Brazil.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100550

Assessment of mutagenic and antimutagenic effects of Punica granatum in mice
Provedor de dados: 60
Autores: Valadares,Marize Campos; Pereira,Enir Raquel Tavares; Benfica,Polyana Lopes; Paula,José Realino.
In the present study, the ability of Punica granatum ethanolic leaf extract (PGL) and Punica granatum ethanolic fruit extract (PGF) to induce mutagenicity or to modulate the genotoxic effects induced by the alkylating agent cyclophosphamide (CP) was evaluated. Swiss male mice were treated by gavage for 10 days with PGL or PGF (12.5, 25, 50, and 75 mg/kg/day) prior to exposure to CP (i.p. 200 mg/kg), 24 h after the end of the treatment. Initial observations revealed that normal mice treated with both extracts (12.5, 25, 50, and 75 mg/kg/day) showed a similar micronucleated polychromatic erythrocyte (MNPCE) frequency to that of the control group. Investigation of the protective effect of PGL and PGF based on data analysis revealed that, irrespective of dose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Punica granatum/mutagenic effects; Micronucleic; Genotoxicity; Cyclophosphamide.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100014

Assessment of analytical techniques for characterization of crystalline clopidogrel forms in patent applications
Provedor de dados: 60
Autores: Lira,Luiz Marcelo; Lourenço,Alexandre Lopes; Neves,Guilherme Ferreira; Sousa,Valéria Pereira de; Rodrigues,Carlos Rangel; Cabral,Lúcio Mendes.
The aim of this study was to evaluate two important aspects of patent applications of crystalline forms of drugs: (i) the physicochemical characterization of the crystalline forms; and (ii) the procedure for preparing crystals of the blockbuster drug clopidogrel. To this end, searches were conducted using online patent databases. The results showed that: (i) the majority of patent applications for clopidogrel crystalline forms failed to comply with proposed Brazilian Patent Office guidelines. This was primarily due to insufficient number of analytical techniques evaluating the crystalline phase. In addition, some patent applications lacked assessment of chemical/crystallography purity; (ii) use of more than two analytical techniques is important; and (iii)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clopidogrel/crystalline forms/physicochemical characterization; Clopidogrel/crystalline forms/patents; Polymorphism.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200229

Development and optimization of erythromycin-loaded lipid-based gel by Taguchi design: In vitro characterization and antimicrobial evaluation
Provedor de dados: 60
Autores: Dhillon,Pallavi; Mirza,Mohd. Aamir; Anwer,Md. Khalid; Alshetaili,Abdullah Saud; Alshahrani,Saad Maria; Iqbal,Zeenat.
The foremost aim of the current research was to prolong and sustain the release of erythromycin (ERY) by preparing a solid lipid nanoparticles (SLNs)-based gel formulation for the safe and effective treatment of acne. ERY-loaded SLNs were developed, and various process variables were optimized with respect to particle size, zeta potential, and entrapment efficiency using the Taguchi model. The average particle size, PDI, zeta potential, drug entrapment efficiency, and drug loading of optimized SLN (F4) were found to be 176.2±1.82 nm, 0.275±0.011, -34.0±0.84, 73.56%, and 69.74% respectively. The optimized SLN (F4) was successfully incorporated into the carbopol-based hydrogel. The in vitro release of ERY from the SLN gel and plain gel were compared and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythromycin; Solid lipid nanoparticles; Nanogel; Taguchi model; Characterization; Diffusion disc.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100583

Study of thermal behavior of phytic acid
Provedor de dados: 60
Autores: Daneluti,André Luis Máximo; Matos,Jivaldo do Rosário.
Phytic acid is a natural compound widely used as depigmenting agent in galenic cosmetic emulsions. However, we have observed experimentally that phytic acid, when heated to 150 ºC for around one hour, shows evidence of thermal decomposition. Few studies investigating this substance alone with regard to its stability are available in the literature. This fact prompted the present study to characterize this species and its thermal behavior using thermal analysis (TG/DTG and DSC) and to associate the results of these techniques with those obtained by elemental analysis (EA) and absorption spectroscopy in the infrared region. The TG/DTG and DSC curves allowed evaluation of the thermal behavior of the sample of phytic acid and enabled use of the non-isothermal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thermal analysis; Phytic acid/thermal behavior; Emulsion/stability; IR/dehydration; EA/thermal decomposition.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200009

Synergistic antibacterial effect of statins with the complex {[1-(4-bromophenyl)-3-phenyltriazene N 3 -oxide-κ 2 N 1 ,O 4 ](dimethylbenzylamine-κ 2 C 1 ,N 4 )palladium(II)}
Provedor de dados: 60
Autores: Rampelotto,Roberta Filipini; Lorenzoni,Vinicius Victor; Silva,Danielly da Costa; Moraes,Guilherme A. de; Serafin,Marissa Bolson; Tizotti,Maísa Kräulish; Coelho,Silvana; Zambiazi,Priscilla; Hörner,Manfredo; Hörner,Rosmari.
Abstract The treatment of infections caused by resistant microorganisms represents a big challenge in healthcare due to limited treatment options. For this reason, the discovery of new active substances which are able to perform innovative and selective actions is of great impact nowadays. Statins and triazenes (TZC) have consolidated as a promising class of compounds, characterized by the expressive biological activity, especially antimicrobial activities. The aim of this study was to assess the in vitro synergistic antibacterial effect of the association of statins and a new TZC complex {[1-(4-bromophenyl)-3-phenyltriazene N 3-oxide-κ 2 N 1,O 4](dimethylbenzylamine-κ 2 C 1,N 4)palladium(II)} (Pd(DMBA)LBr) against American Type Culture Collection (ATCC)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statins/antimicrobial activity; Triazenide; Atorvastatin; Simvastatin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200614

Antidiabetic activity evaluation of Onobrychis species on alloxan-induced diabetic mice
Provedor de dados: 60
Autores: Ozbek,Hanefi; Acikara,Ozlem Bahadir; Oz,Burcin Ergene; Ozbilgin,Serkan; Kirmizi,Neriman Ipek; Ozrenk,Bade Cevriye; Tekin,Mehmet; Saltan,Gulcin.
In the current study, four Onobrychis species, O. albiflora Hub.-Mor., O. argyrea Boiss. subsp. argyrea Boiss., O. galegifolia Boiss., and O. tournefortii (Willd.) Desv. were collected from Anatolia to be evaluated for their antidiabetic activities. Methanol water extracts of the aerial parts were used for experiments. An alloxan-induced diabetic mice test model was used. Phytochemical analysis of the tested extracts was investigated using the HPLC method. The highest activity was observed with treatment of O. albiflora aerial part extract. Significant decrements were detected in the blood glucose levels as follows: 180.83±47.48 and 252.83±50.47mg/dL at 100 mg/kg and 200 mg/kg doses of O. albiflora, respectively, when compared to the isotonic saline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Onobrychis; Antidiabetic activity; Alloxan; Rutin; Isoquercetin; Phytochemical analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100504

The effect of single-bulb garlic oil extract toward the hematology and histopathology of the liver and kidney in mice
Provedor de dados: 60
Autores: Lestari,Sri Rahayu; Rifai,Muhaimin.
Single garlic has been used as a traditional medicine for hypercholesterolemia and increasing stamina. The aim of this research was to analyze the effect of single-bulb garlic oil (SGO) extract toward hematology and histopathology liver and kidney in mice. Male mice (strain Balb-C, 12 weeks old, weight of 28±3 g) was treated by SGO for 28 days (once per days) with various doses K- (Normal); K+ (SGO 0.0 mg/kg BW); T1 (SGO 6.25 mg/kg BW); T2 (SGO 12.5 mg/kg BW); T3 (SGO 25.0 mg/kg BW); T4 (SGO 50.0 mg/kg BW). The end of the treated mice was sacrificed. Blood was collected to analyze hematology. Liver and kidney were collected then made microanatomy slide with Hematoxylin-Eosin staining. Parameters of hematology are the levels of hemoglobin, hematocrit,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematology; Histopathology; Single-bulb garlic oil extract.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100560

Enzymes modulation by dried grape pomace from the manufacture of wines and juices
Provedor de dados: 60
Autores: Freire,Juliana Mesquita; Barroso,Amanda Ribeiro; Assis,Amanda Araújo de; Texeira,Bruna Helena; Braga,Jonatas Henrique Guimarães; Oliveira,Daniela Aparecida; Braga,Mariana Aparecida; Marcussi,Silvana.
The processing of grapes for the manufacture of juices and wines, generates large quantities of by-products rich in metabolites with antioxidant, antimicrobial, anti-inflammatory and cicatrizing activities. The high homology between human enzymes and snake venoms makes the latter valuable laboratory tools for the study of pathophysiological processes. Proteases and phospholipases A2 act in processes related to hemostasis and inflammatory response. Thus, in this work, dried pomace obtained from grape (Isabel, Niagara, Bordô, BRS Violeta and Blend cultivars) processing were evaluated on phospholipase, proteolytic, hemolytic and thrombolytic activities induced by snakes venoms and the content of phenolic compounds and minerals was evaluated. The dried pomace...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grape extracts; Industrial wastes; Phenolic compounds; Protease modulators; Phospholipase A2 modulators.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100609

Chemical degradation kinetics of fibrates: bezafibrate, ciprofibrate and fenofibrate
Provedor de dados: 60
Autores: Oliveira,Marcelo Antonio de; Silva,Gerliane Damázio da; Campos,Michele Soares Tacchi.
ABSTRACT Fibrates are drugs used for the treatment of hypertriglyceridemia and for the prevention of atherosclerosis. Three drugs in the fibrate class, ciprofibrate, fenofibrate and bezafibrate, were chosen for this study because their raw materials are readily available and because scientific publications on these compounds is limited. To evaluate their intrinsic stability, the drugs were exposed to a test condition (temperature, oxidation, UV light exposure, hydrolysis at different pH values and metal ions in solution) and then were subjected to analysis by HPLC. The samples were run on a C18 column, with a flow rate of 1.0 mL min-1 in a mobile phase consisting of methanol: 0.01 % phosphoric acid v/v (80:20), with variable detection wavelengths in the UV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertriglyceridemia/fibrates; Fibrates stability/study; Fibrates degradation products/High Perfomance Liquid Chromatography (HPLC); Fibrates/Degradation kinetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300545

Validation of a UV-spectrophotometric analytical method for determination of LPSF/AC04 from inclusion complex and liposomes
Provedor de dados: 60
Autores: Ferraz,Rafaela Siqueira; Mendonça,Elisângela Afonso Moura; Silva,Jéssica Priscila Avelino; Cavalcanti,Isabella Macário Ferro; Lira-Nogueira,Mariane Cajubá Britto; Galdino,Suely Lins; Pitta,Ivan Rocha; Lima,Maria do Carmo Alves; Santos-Magalhães,Nereide Stela.
The aim of this study was to develop and validate a UV spectrophotometric method for determination of LPSF/AC04 from inclusion complex and encapsulated into liposomes. The validation parameters were determined according to the International Conference on Harmonisation (ICH) and National Health Surveillance Agency (ANVISA) guidelines. LPSF/AC04 was determined at 250 nm in methanol by a UV spectrophotometric method, exhibiting linearity in the range from 0.3 to 2 µg.mL−1 (Absorbance=0.18068 x [LPSF/AC04 µg.mL-1] + 0.00348), (r2=0.9995). The limits of detection and quantification were 0.047µg.mL−1 and 0.143µg.mL−1, respectively. The method was accurate, precise, reproducible and robust since all the samples analyzed had coefficient of variation of less than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: UV Spectrophotometry/quantitative analysis/method validation; Acridine derivatives/determination; Inclusion complex; Liposomes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100183

Development and validation of analytical methodology with focus on the qualification of powder mixers
Provedor de dados: 60
Autores: Fiorante,Pedro de Freitas; Martins,Rodrigo Dias; Palma,Mauri Sergio Alves.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Powder mixers/analytical validation; Spectrophotometry; Ferric oxide/quantification; Lactose monohydrate/quantitative analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200273

Choice of sterilizing/disinfecting agent: determination of the Decimal ReductionTime (D-Value)
Provedor de dados: 60
Autores: Mazzola,Priscila Gava; Jozala,Angela Faustino; Novaes,Letícia Célia de Lencastre; Moriel,Patricia; Penna,Thereza Christina Vessoni.
Efforts to diminish the transmission of infections include programs in which disinfectants play a crucial role. Hospital surfaces and medical devices are potential sources of cross contamination, and each instrument, surface or area in a health care unit can be responsible for spread of infection. The decimal reduction time was used to study and compare the behavior of selected strains of microorganisms. The highest D-values for various bacteria were obtained for the following solutions: (i) 0.1% sodium dichloroisocyanurate (pH 7.0) - E. coli and A. calcoaceticus (D = 5.9 min); (ii) sodium hypochlorite (pH 7.0) at 0.025% for B. stearothermophilus (D = 24 min), E. coli and E. cloacae (D = 7.5 min); at 0.05% for B. stearothermophilus (D = 9.4 min) and E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disinfection; Disinfectant agents; Decimal reduction time/determination.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400013

Effect of the addition of inulin on the nutritional, physical and sensory parameters of bread
Provedor de dados: 60
Autores: Brasil,Jacira Antonia; Silveira,Karina Correia da; Salgado,Silvana Magalhães; Livera,Alda Verônica Souza; Faro,Zelyta Pinheiro de; Guerra,Nonete Barbosa.
The present work evaluated the effect of the addition of inulin on sensory, nutritional and physical parameters of white bread. Three formulations containing 0%, 6% and 10% of inulin were produced. Physical analyses of mass, volume, specific volume, density, centesimal composition, glycemic index (GI) and qualitative descriptive sensory analyses, were carried out. The reduction in bread volume seen with 10% inulin was higher than that reported in the literature. Bread weight did not differ statistically. There was an 85% reduction in fat content for bread with 6% inulin and 86% for bread containing 10% inulin. Glycemic index in bread containing 10% inulin was lower than bread with 6% inulin and equal to inulin-free bread. A level of 6% inulin added to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inulin/effect of application; White bread/analysis; Fat/substitutes; Foods/sensory analyse.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100023

In vitro cytotoxic activity of five commercial samples of Tribulus terrestris Linn in Espírito Santo (Brazil)
Provedor de dados: 60
Autores: Oliveira Filho,Claudio Costa; Kampke,Edgar Hell; Vargas,Thais Souza; Salustriano,Nathacha Andrade; Scherer,Rodrigo; Fronza,Marcio; Campagnaro,Bianca Prandi.
ABSTRACT The objective was to investigate the total saponin and protodioscin concentrations and the cytotoxicity in vitro, of five samples of the plant Tribulus terrestris, commercially available in the metropolitan region of Vitória - Espirito Santo, Brazil, and to compare them with the aqueous extract of the plant. The chromatographic profile and quantification of protodioscin in commercial samples and plant extract were evaluated by LC-MS/MS. The percentage of total saponins were determined by the colorimetric method. Extracts and protodioscin cytotoxicity were analyzed by the MTT assay in three cell lineages: fibroblasts (L929), ovarian cancer (Ovcar3) and murine hepatoma (Hepa1c1c7). All extracts displayed high levels of total saponins (207.2 to 780.3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tribulus terrestres/saponin/protodioscin/concentrations; Medicinal plants; Cytotoxicity/in vitro.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400622

Pesticide use and cholinesterase inhibition in small-scale agricultural workers in southern Brazil
Provedor de dados: 60
Autores: Nerilo,Samuel Botião; Martins,Fernanda Andrade; Nerilo,Luciana Botião; Salvadego,Valter Eduardo Cocco; Endo,Renan Yoshio; Rocha,Gustavo Henrique Oliveira; Mossini,Simone Aparecida Galerani; Janeiro,Vanderly; Nishiyama,Paula; Machinski Junior,Miguel.
A controlled cross-sectional study of family growers of fruit and vegetables was conducted between October 2009 and October 2010 to characterize the use of pesticides, establish the socio-demographic profile, and analyze cholinesterase activity in small-scale agricultural workers in Southern Brazil. Data was collected for 173 workers and 179 controls. A structured questionnaire was applied collecting socio-demographic information and determining knowledge and work practices in relation to pesticide use. The benchmarks for total cholinesterase (ChEs) and butyrylcholinesterase (BuChE) were obtained from the average enzymatic activity of the occupationally unexposed group (control). The mean age of the exposed population was 40.0 ± 11.2 years. The symptoms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticides/use; Pesticides/occupational exposure; Cholinesterase/evaluation/exposure to pesticides; Agricultural workers/exposure to pesticides; Environmental health; Brazil/southern/environmental health.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400783

The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum
Provedor de dados: 60
Autores: Taşkın,Turgut; Gezmiş,Tuğba; Çam,Muhammet Emin; Taşkın,Duygu; Çelik,Berna Özbek; Şenkardeş,İsmail; Süzgeç-Selçuk,Sevda.
In Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones are endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo anti-inflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helichrysum plicatum subsp; Plicatum; Anti-inflammatory; Anti-urease; Anticholinesterase; HPLC-DAD-MS.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100554

Antidiabetic and antihyperlipidemic effects of Dillenia indica (L.) leaves extract
Provedor de dados: 60
Autores: Kumar,Sunil; Kumar,Vipin; Prakash,Om.
The present study was carried out to evaluate antidiabetic and antihyperlipidemic effects of Dillenia indica methanolic leaves extracts in streptozotocin induced diabetic Wistar rats by administering graded oral doses (250 and 500 mg/kg body weight) for 21 days. The extract showed significant antidiabetic activity (p&lt;0.001). Furthermore, the decreased body weight of rats was significantly improved after extract treatments. Daily oral treatment with the extract for 21 days to diabetic rats, also resulted in significant reduction in serum cholesterol, triglycerides and serum transaminase levels but HDL-cholesterol level was found to be improved (p&lt;0.001) as compared to the diabetic control group. The extract treatment also showed to enhance serum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dillenia indica/antidiabetic effects; Dillenia indica/experimental study; Dillenia indica/antihyperlipidemic effects; Dillenia indica/experimental study; Antidiabetics/experimental study; Serum transaminase; Serum cholesterol; HDL-cholesterol level.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200018

Synthesis and biological evaluation of 125I-erythropoietin as a potential radiopharmaceutical agent for tumours
Provedor de dados: 60
Autores: Clemente,Gonçalo dos Santos; Duarte,Vera Lúcia Serra.
Erythropoietin (EPO) is a glycoprotein hormone responsible for regulating erythropoiesis. Expression of EPO and EPO receptors (EPOr) has recently been demonstrated in some neoplastic cell lines and tumours, suggesting a potential new target for therapy. In this work, EPO was labeled with iodine-125 using the lactoperoxidase method, known to prevent damage to protein during radioiodination, and labeling conditions were optimized. In vitro stability studies have shown that 125I-EPO is radiochemically stable for 20 days after radiolabeling. In vitro cell binding studies have demonstrated very low binding (&lt;2%) of EPO to normal and neoplastic cell lines tested. As expected, the biodistribution in healthy mice exhibited comparatively high rates of fixation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythropoietin (EPO); Glycoprotein hormone; 125I-erythropoietin/synthesis; 125I-erythropoietin/biological evaluation; Erythropoiesis/regulation; Radiopharmaceuticals; Iodine-125/labelled; EPO expressive tumours.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100010

Effect of probiotics on giardiasis. Where are we?
Provedor de dados: 60
Autores: Ventura,Luciana Laranjo Amorim; Oliveira,Dirce Ribeiro de; Gomes,Maria Aparecida; Torres,Marcia Regina Fantoni.
ABSTRACT Giardiasis, an intestinal infection caused by Giardia lamblia, was recently included in the ‘Neglected Diseases Initiative’ by the World Health Organization. Symptomatic patients can develop mild diarrhea up to a severe malabsorption syndrome, and children may show significantly impaired cognitive and physical development. Currently, nitroimidazoles are the main class of antibiotic used to treat giardiasis. Despite the efficacy of these drugs, adverse effects and reported resistance have increased, encouraging studies to identify and develop therapeutic alternatives. In this context, probiotics may represent an attractive option. Probiotics are defined as live microorganisms that, when administered in suitable amounts, confer a health benefit to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giardia lamblia; Giardiasis; Probiotics/effects/evaluation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200401

Glutamine dipeptide supplementation improves clinical responses in patients with diabetic foot syndrome
Provedor de dados: 60
Autores: Krupek,Tuane; Silva,Maria Angélica Rafaini Covas Pereira da; Sant´Ana,Débora de Mello Gonçalves; Batista,Márcia Regina; Shimauti,Eliana Litsuko Tomimatsu; Sá-Nakanishi,Anacharis Babeto de; Comar,Jurandir Fernando; Schamber,Christiano Rodrigues; Moreschi Junior,Dorival; Bazotte,Roberto Barbosa.
ABSTRACT The effect of glutamine dipeptide (GDP) supplementation in patients with diabetic foot syndrome was evaluated. A total of 22 patients took part in the study. GDP was supplied in 10 g sachets, and was dissolved in water immediately before use, with ingestion once a day, after lunch or after dinner (20 g/day) over a period of 30 days. Quantification of foot insensitive areas, oxidative stress, blood cytokines, and biochemical, hematological and toxicological parameters was performed before and after GDP supplementation. We observed an increase in blood levels of interferon-α (P=0.023), interferon-γ (P=0.038), interleukin-4 (P=0.003), interleukin-6 (P=0.0025), interleukin-7 (P=0.028), interleukin-12 p40 (P=0.017), interleukin-13 (P=0.001), leukocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus/treatment/study; Glutamine/effects; Nutraceuticals.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300567

Formulation, characterization and in vivo evaluation of novel edible dosage form containing nebivolol HCl
Provedor de dados: 60
Autores: Vijayanand,Pujari; Patil,Jagadevappa; Reddy,Manda Venkata.
ABSTRACT The objective of this investigation was to develop a novel oral edible gel dosage form for nebivolol hydrochloride, with suitable rheological characteristics that can provide a means of administering the drug to dysphagic and geriatric patients. Edible gels were prepared using low acetylated gellan gum and sodium citrate in different concentrations. The effect of concentration of the solution on gelation time, viscosity, and drug release was studied. Optimized formulation had "spoon thick" consistency that is considered suitable for dysphagic patients as suggested by National Dysphagia Diet Task Force. The optimized formulation containing gellan gum (0.4 % w/v) and sodium citrate (0.3 % w/v) showed more than 95% drug release in 20 minutes. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edible gel/pharmaceutics; Nebivolol HCl/delivery; Dysphagia/drugs administration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100020

Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice
Provedor de dados: 60
Autores: Mancini,Dalva Assunção Portari; Torres,Rosângela Pavan; Pinto,José Ricardo; Mancini-Filho,Jorge.
This work aimed to evaluate antiviral properties in antioxidants from spices. Phenolic compounds extracted from rosemary (Rosmarinus officinallis, L) by hot water, had their antioxidant activity determined by spectrophotometry using β carotene/linoleic acid system. The rosemary extract was evaluated by antiviral assay of Herpes Virus type-1 (HSV-1) replication in VERO cells, in the presence or absence of the spice. 10,000 TCID50/mL of the HSV-1 was kept for 3 h at 4º C, with 300 ppm of rosemary extract, and 100 ppm of butyl hydroxyl toluene (BHT). Then, these viruses were inoculated in VERO cells incubated at 37º C in CO2-5 %, for seven days. Daily, they were examined and the end point was based on 100% of CPE in virus control (without antioxidants). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural antioxidants; Virus replication; Phenolic compounds; Herpesvirus; Rosemary.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100016

Analysis of the evidence of efficacy and safety of over-the-counter cough medications registered in Brazil
Provedor de dados: 60
Autores: Reis,Adriano Max Moreira; Figueras,Albert.
The objective of this study was to analyze the level of evidence regarding the efficacy, effectiveness and safety of over-the-counter (OTC) cough medications registered in Brazil. The National Health Surveillance Agency database was used to identify the drugs. Clinical trials, systematic reviews, meta-analyses, and studies on safety were searched on the Medline baseline, the Cochrane Library and SIETES (System of Essential Information in Therapeutics and Health; database in Spanish). Most drugs (62.5%) were sold as a fixed-dose combination of two or more drugs. Randomized clinical trials were found for only three drugs: bromhexine, dextromethorphan and guaifenesin. No clinical trials were found for fixed-dose combinations. Systematic reviews on Cochrane...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/utilization studies; Cough; Drugs/nonprescription; Pharmacovigilance; Pharmacoepidemiology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100016

Effect of iguratimod on diclofenac metabolism by CYP2C9 in rats and human recombinant CYP2C9 yeast cells
Provedor de dados: 60
Autores: Usman,Muhammad; Zhen-Han,Zhu; Ze-Na,Chang; Jun-Ping,Han; Wen,Qian; Chang-Qing,Yang; Miyu,Nishikawa; Toshiyuki,Sakaki.
Iguratimod (IGU, also known as T-614), a novel disease modifying antirheumatic drug intended to cure patients with rheumatoid arthritis (RA). The purpose of this study is to evaluate the effect of IGU on the pharmacokinetics of CYP2C9 probe drug diclofenac and its metabolite 4′-hydroxy diclofenac in vivo and in vitro. In in vivo experiments, 24 rats were randomly assigned to three groups consisting of the control group (Normal saline), low dose IGU group (10 mg/kg) and high dose IGU group (30 mg/kg). Blood samples were collected from orbital sinuses vein before 1 hour and serial times of giving diclofenac (15 mg/kg) to all the rats. Plasma concentration of diclofenac and its metabolite 4´-hydroxy diclofenac were assayed by high performance liquid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iguratimod/effects; Diclofenac/effects; 4-hydroxy diclofenac; CYP2C9/pharmacokinetics; Inhibitory.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100523

Development of a dissolution test for lamotrigine in tablet form using an ultraviolet method
Provedor de dados: 60
Autores: Martins,Magda Targa; Paim,Clésio Soldatelli; Steppe,Martin.
A dissolution test for tablets containing 100 mg of lamotrigine was developed and validated. The dissolution test was applied to compare the dissolution profile of Neural® with the reference product Lamictal®. The analysis procedure was carried out using a simple ultraviolet method at 267 nm. After the determination of solubility and sink conditions, the parameters selected were paddles at 50 rpm, 900 mL of 0.01 M hydrochloric acid, and 30 minutes duration (single point). This method was validated for specificity, linearity, accuracy, precision and robustness. Lamotrigine stability was also evaluated in dissolution medium.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lamotrigine; Dissolution test/validation; Medicines/quality control.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200003

Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation
Provedor de dados: 60
Autores: Amparo,Tatiane Roquete; Rodrigues,Ivanildes Vasconcelos; Seibert,Janaína Brandão; Almeida,Tamires Cunha; Cabral,Vivette Appolinário Rodrigues; Vieira,Paula Melo de Abreu; Brandão,Geraldo Célio; Oliveira,Mauro Lúcio Gonçalves de; Silva,Glenda Nicioli da; Santos,Orlando David Henrique dos; Vieira Filho,Sidney Augusto; Teixeira,Luiz Fernando Medeiros; Souza,Gustavo Henrique Bianco de.
Due to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria. By means of diffusion and microdilution assays, the crude extract was active against the nine bacteria tested being the hydromethanolic fraction the most active. During phytochemical procedures, procyanidin (1) and catechin (2) were identified as the main antibacterial constituents of this fraction. In silico results obtained using PASSonline tool indicated 1 and 2 as having good potential to interact with different targets of currently used antibiotics. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protium spruceanum; Burseraceae; Procyanidin; Catechin; Antibacterial activity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100623

Ultraviolet spectrophotometric method for analytical determination of mianserin hydrochloride in coated tablets and comparison with LC
Provedor de dados: 60
Autores: Sfair,Letícia Lenz; Graeff,Jeferson Scarpari; Steppe,Martin; Schapoval,Elfrides Eva Scherman.
abstract Ultraviolet spectrophotometric (UV) and Liquid Chromatographic (LC) methods for the determination of mianserin hydrochloride in pharmaceutical formulation were developed and validated. The various parameters, such as specificity, linearity, precision and accuracy were studied according to International Conference on Harmonization (ICH, 2005). For UV method, mianserin hydrochloride was determinate at 278 nm using HCl 0.1 M as the solvent. The response was linear in the concentration range of 20.0 - 140.0 µg/mL (r = 0.9998). Precision data evaluated by relative standard deviation was lower than 2%. The UV method was simple, rapid and low cost. Chromatographic analyses were performed in an Ace C18 column and the mobile phase was composed of methanol,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultraviolet spectrophotometry/qualitative analysis; Liquid chromatographic/qualitative analysis; Mianserin hydrochloride/determination in tablets; Coated tablets/qualitative analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400833

Ustekinumab treats psoriasis by suppressing RORC and T-box but its suppression of GATA restrains its efficacy
Provedor de dados: 60
Autores: Liu,Lun-Fei; Chen,Ji-Su; Shen,Ji-Yang; Dou,Ting-Ting; Zhou,Jiong; Cai,Sui-Qing; Zheng,Min.
Psoriasis is a T-cell mediated disease that involves IL-23/Th17 and IL-12/Th1 axes. Ustekinumab, a fully human monoclonal antibody targeting the p40 subunit of both IL-12 and IL-23, has proven to be efficient and safe for treating patients with psoriasis. Yet, there have been no reports with human skin/blood samples that would elucidate the molecular mechanisms by which ustekinumab calms psoriasis skin lesions. To investigate the efficacy and molecular pathway (RORC, t-BOX and GATA) of ustekinumab in treating patients with psoriasis skin lesions. A total of 30 patients with psoriasis were randomized into placebo group and treatment group. PASI of each patient was calculated at 0, 12 and 24 weeks post-treatment. The mRNA levels of RORC, t-BOX and GATA in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psoriasis; Ustekinumab/efficacy; Biologic agents.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400615

Tissue injuries of wistar rats treated with hydroalcoholic extract of Sonchus oleraceus L.
Provedor de dados: 60
Autores: Prichoa,Franciele Carla; Roman,Silvane Souza; Manfredini,Vanusa.
The use of plant species is emerging as an important alternative in the treatment of injuries. Therefore, the extract of Sonchus oleraceus 10% was employed in the repair of skin lesions. A total of 36 male Wistar rats were subjected to a punch injury and divided into three groups: a negative control, receiving no treatment, a positive control, treated with Dersani, and the experimental group treated with the extract. The injury was assessed macroscopically and microscopically. Morphometric data was collected at the 3rd, 5th and 7th postoperative day, and the experimental group showed greater changes in shrinkage of the lesion compared to control groups. On the 3rd postoperative day, the injury in the experimental group showed less necrotic tissue, lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sonchus oleraceus/pharmacognosy; Skin lesions/treatment; Skin lesions/experimental study; Wound healing/experimental study; Medicinal plants.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300019

Toxicology and safety of the tincture of Operculina alata in patients with functional constipation
Provedor de dados: 60
Autores: Santos,Luciana Kelly Ximenes dos; Cunha,Gilmara Holanda da; Fechine,Francisco Vagnaldo; Pontes,Andréa Vieira; Oliveira,Jonaina Costa de; Bezerra,Fernando Antonio Frota; Moraes,Manoel Odorico de; Moraes,Maria Elisabete Amaral de.
The tincture of Operculina alata, popularly known as "tincture of jalapa", is used in Northeast Brazil to treat constipation and encephalic vascular accident, but it has not yet been adequately tested for safety and efficacy. The aim of this study was to evaluate the toxicology and safety of the tincture of O. alata in patients with functional constipation. This was a double-blind, randomized, placebo-controlled clinical trial. The study consisted of three phases: pre-treatment, treatment and post-treatment, each phase with duration of seven days. Arterial pressure, heart rate, body weight, adverse events, hematological, metabolic, liver and kidney functions were monitored. Forty patients were randomized to receive tincture of O. alata and 43 patients to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Operculina alata/pharmacognosy; Operculina alata/tincture; Operculina alata/toxicological evaluation; Operculina alata/tincture; Operculina alata/safety; Tincture of Jalapa see Operculina alata/tincture.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300014

Pharmaceutical care as a strategy to improve the safety and effectiveness of patients? pharmacotherapy at a pharmacy school: a practical proposal
Provedor de dados: 60
Autores: Chemello,Clarice; Souza,Fernanda de; Patricio,Eliziane de Souza; Farias,Mareni Rocha.
Several patients experience at least one drug-related problem and Pharmaceutical Care can change this reality. This work describes a model for structuring the pharmaceutical care service at a pharmacy training unit of the Brazilian Public Health System based on pharmacotherapy follow-up program of Parkinson’s disease patients’ results. From the follow-up results (phase 1), a Therapy Management Scheme was designed (phase 2). Of the 57 patients followed-up, 30 presented at least one drug-related problem and 42% were non-adherent to treatment, which supported the need of pharmacotherapy management. The Pharmacotherapy Management Scheme was proposed as a pharmaceutical care service model, which presents 6 steps: first, the pharmacist fills out the dispensing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care; Pharmacotherapy follow-up; Community pharmacy; Patients/safety; Parkinson?s disease/treatment.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100019

Recovery of drug residues in equipment and utensils used by compounding pharmacies after standard cleaning procedure
Provedor de dados: 60
Autores: Prioste,Tassia; Magon,Thiago Ferreira dos Santos; Fagundes,Vinicius; Montanha,Maiara Camotti; Moriwaki,Cristiane; Kimura,Elza.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compounding pharmacy/cleaning procedure/equipments; Pharmacy equipaments/cleaning; Pharmaceutical products/contamination; Captopril; Acetylsalicylic acid; Hydrochlorothiazide.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200317

Presence of benznidazole conjugated metabolites in urine identified by β-glucuronidase treatment
Provedor de dados: 60
Autores: Marson,María Elena; Bournissen,Facundo García; Altcheh,Jaime; Moscatelli,Guillermo; Moroni,Samantha; Mastrantonio,Guido Enrique.
Chagas disease is a serious public health problem in Latin America and, due to migration, in other non-endemic regions. Benznidazole (BNZ) is first choice drug in pediatric therapeutics. However, little is known regarding its metabolism in humans. The aim of the study was to isolate and identify products of human BZN metabolism in urine samples obtained from a pediatric Chagas patient and a healthy adult volunteer both treated with BZN. Urine samples were collected after dose of BNZ. Urine was treated with β-glucuronidase followed by an extraction procedure under two different pH conditions and a HPLC/UV and MS/MS identification of BZN and its metabolites. BZN (m/z 260.09847) was identified in all urine extracts. Peaks from each extracted chromatograms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benznidazole metabolites; Chagas Disease/drug therapy; Glucuronidase/urine; High Performance Liquid Chromatography HPLC/methods; Mass Spectrometry/ methods; Antiparasitic agents/pharmacology.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100520

Simultaneous determination of anti-diabetic drugs
Provedor de dados: 60
Autores: Sher,Nawab; Fatima,Nasreen; Perveen,Shahnaz; Siddiqui,Farhan Ahmed.
A novel reverse phase, isocratic HPLC method is described to separate five anti-diabetic drugs i.e., glimepiride, metformin, sitagliptin, rosiglitazone and pioglitazone. Nucleosil C18 analytical column was used as stationary phase, while mobile phase consisted of acetonitrile:phosphate buffer: methanol (40/40/20, v/v) pH 2.0. Effluent was monitored at a flow rate 1 mL/min and detected at wavelength of 240 nm. This research produced excellent chromatography over a wide concentration range of 25-10000 ng/mL. Sepprated and well resolved quantifiable peaks were obtained and test results were linear in this range. Correlation coefficient of more than 0.9990 was witnessed as well as Low %RSD values i.e., maximum 2.0% documented excellent precision of the method....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metformin/pharmacokinetics High Pressure Liquid Chromatography; Urine/Plasma methods Determination/prevention Diabetes Mellitus/drug therapy Drug Liberation/drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100582

Hydrolyzed collagen interferes with  in vitro photoprotective effectiveness of sunscreens
Provedor de dados: 60
Autores: Peres,Daniela D'Almeida; Hubner,Alexandra; Oliveira,Camila Areias de; Almeida,Tania Santos de; Kaneko,Telma Mary; Consiglieri,Vladi Olga; Pinto,Claudinéia Aparecida Sales de Oliveira; Velasco,Maria Valéria Robles; Baby,André Rolim.
ABSTRACT The chronological skin aging is a progressive and natural process with genetic and physiological changes. However, ultraviolet (UV) radiation may accelerate the oxidative stress, generating carcinogenesis and photoaging. Natural compounds and their applications are considered a trend in the cosmetic market. The protein-based film-forming compounds play an important role, once it collaborates for the better distribution of sunscreens on the skin. Here we investigated the in vitro photoprotective effectiveness of sunscreens containing the hydrolyzed collagen associated with UVA, UVB and/or inorganic filters. Sunscreens were developed with octocrylene (7.5%), butyl methoxydibenzoylmethane (avobenzone) (3.0%) and/or titanium dioxide (5.0%), associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrolyzed collagen/protective effects; Photoprotector/effectiveness; Photoprotector/in vitro study; Sun protection factor; Sunscreen/UV filter; Critical wavelength.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200611

Cyclodextrins and ternary complexes: technology to improve solubility of poorly soluble drugs
Provedor de dados: 60
Autores: Miranda,Janisse Crestani de; Martins,Tércio Elyan Azevedo; Veiga,Francisco; Ferraz,Humberto Gomes.
Cyclodextrins (CDs) are cyclic oligosaccharides composed of D-glucopyranoside units linked by glycosidic bonds. Their main property is the ability to modify the physicochemical and biological characteristics of low-soluble drugs through the formation of drug:CD inclusion complexes. Inclusion complexation requires that host molecules fit completely or partially within the CD cavity. This adjustment is directly related to the physicochemical properties of the guest and host molecules, easy accommodation of guest molecules within the CD cavity, stoichiometry, therapeutic dose, and toxicity. However, dosage forms may achieve a high volume, depending on the amount of CD required. Thus, it is necessary to increase solubilization efficiency in order to use...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclodextrins; Ternary complexes; Drugs/complexation; Water-soluble polymers/use; Drugs/solubility; Inclusion complexe.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400003

Quantitative analysis of phenobarbital in biological fluids: Analyte enrichment by an electrically-assisted microextraction technique
Provedor de dados: 60
Autores: Yaripour,Saeid; Ebrahimi,Shahriar; Mohammadi,Ali.
An electrically-assisted microextraction method called electromembrane extraction, followed by a simple high performance liquid chromatography and ultraviolet detection was developed and validated for determining phenobarbital in biological samples. The major parameters influencing the electromembrane extraction procedure including solvent composition, voltage, pH of acceptor and donor solutions, salt effect, and time of extraction were evaluated and optimized. The drug was extracted from the donor aqueous sample solution (pH 9) to the acceptor aqueous solution (pH 13). The donor and acceptor phases were separated by a hollow fiber dipped in 1-octanol as a supported liquid membrane. A voltage of 40 V during 20 minutes was applied as the driving force. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological samples; Electromembrane extraction; HPLC-UV; Microextraction; Phenobarbital.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100543

Impact of a drug dispensing model at a Community Pharmacy in Goiânia, Goiás, Brazil
Provedor de dados: 60
Autores: Ferreira,Tatyana Xavier Almeida Matteucci; Prudente,Luciana Resende; Dewulf,Nathalie de Lourdes Souza; Provin,Mércia Pandolfo; Cardoso,Thaissa Costa; Silveira,Érika Aparecida da; Amaral,Rita Goreti.
Although dispensing of medication has been addressed by theoretical models, studies that confirm the impact of this service are still needed. The objective was to evaluate the impact of a new model of medicine dispensing system on patients’ medication knowledge, adherence to treatment and satisfaction. One hundred and four patients attending the dispensing service of a community pharmacy between 21 January 2013 and 20 April 2013 were included in this intervention study. The impact of the service on patients’ medication knowledge, adherence to treatment and satisfaction was assessed by using validated questionnaires at two time points: at the moment of medication dispensing and 30 days thereafter by telephone contact. Statistical analysis was performed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community Pharmacy Services; Outcome Assessment (Health Care); Pharmacies; Patient Medication Knowledge; Patient Satisfaction; Brazil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400602

Neuroprotective and MAOB inhibitory effects of a series of caffeine-8-thioglycolic acid amides
Provedor de dados: 60
Autores: Kasabova-Angelova,Alexandra; Kondeva-Burdina,Magdalena; Mitkov,Javor; Georgieva,Maya; Tzankova,Virginia; Zlatkov,Alexander.
The effects of new derivatives of caffeine-8-thioglycolic acid (100 µM) on isolated rat brain synaptosomes, human neuroblastoma cell line SH-SY5Y and human recombinant MAOB enzyme (hMAOB) (1 µM) were evaluated. Most of the compounds, administered alone, didn’t show statistically significant neurotoxic effects on SH-SY5Y, when compared to the control (non-treated cells). Of all studied structures JTA-2Ox, JTA-11, JTA-12 and JTA-13 decreased cell viability. In combination with 6-hydroxydopamine (6-OHDA) (100 µM), only JTA-1 and JTA-2 revealed neuroprotective effects, stronger than those of caffeine. All compounds administered alone revealed, neurotoxic effects on synaptosomes, as compared to non-treated synaptosomes. JTA-1, JTA-2 and JTA-3 showed lowest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroprotection; Caffeine; Synaptosomes; SH-SY5Y; HMAOB.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100529

Antioxidant and cytotoxic properties of protein hydrolysates obtained from enzymatic hydrolysis of Klunzinger’s mullet (Liza klunzingeri) muscle
Provedor de dados: 60
Autores: Rabiei,Sana; Rezaei,Masoud; Asgharzade,Samira; Nikoo,Mehdi; Rafieia-kopai,Mahmoud.
Today, consumers are looking for functional foods that promote health and prevent certain diseases in addition to provide nutritional requirements. This study aimed to evaluate the antioxidant and cytotoxic properties of Liza klunzingeri protein hydrolysates. Fish protein hydrolysates (FPHs) were prepared from L. klunzingeri muscle using enzymatic hydrolysis with papain at enzyme/substrate ratios of 1:25 and 1:50 for 45, 90 and 180 min. The antioxidant activities of the FPHs were investigated through five antioxidant assays. The cytotoxic effects on 4T1 carcinoma cell line were also evaluated. The amino acid composition and molecular weight distribution of the hydrolysate with the highest antioxidant activity were determined by HPLC. All six FPHs exhibited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant activity; Cytotoxic effect; Protein hydrolysate; Liza klunzingeri.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100569

Guava: phytochemical composition of a potential source of antioxidants for cosmetic and/or dermatological applications
Provedor de dados: 60
Autores: Chiari-Andréo,Bruna Galdorfini; Trovatti,Eliane; Marto,Joana; Almeida-Cincotto,Maria Gabriela José de; Melero,Ana; Corrêa,Marcos Antonio; Chiavacci,Leila Aparecida; Ribeiro,Helena; Garrigues,Teresa; Isaac,Vera Lucia Borges.
ABSTRACT Guava (Psidium guajava L.) is a native fruit of the American tropics with commercial applications for its taste, flavor and aroma. Numerous pharmacological uses have been described for it, such as the antiseptic effect of its leaves, the use of the fresh fruit and tea from its leaves for the treatment of diarrhea, dysentery, diabetes mellitus, and others. However, considering its rich composition, the guava also is a potential source of antioxidants to be used in the development of new formulations for cosmetic and/or dermatological applications, the main focus of this research. Herein, we describe the study of the phytochemical composition and the antioxidant activity of a guava extract prepared with non-toxic solvents aiming its use at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P. guajava L./phytochemistry; P. guajava L./use in cosmetics; P. guajava L./antioxidant/chemical composition; P. guajava L/chemical stability; High Performance Liquid Chromatography.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200612

Medication dispensing as an opportunity for patient counseling and approach to drug-related problems
Provedor de dados: 60
Autores: Ferreira,Tatyana Xavier Almeida Matteucci; Prudente,Luciana Resende; Dewulf,Nathalie de Lourdes Souza; Provin,Mércia Pandolfo; Mastroianni,Patrícia de Carvalho; Silveira,Erika Aparecida da; Amaral,Rita Goreti.
ABSTRACT The objective was to describe and evaluate a model of drug dispensing developed and implemented in a community pharmacy in Brazil. This was a descriptive, observational, quasi-experimental study performed in the period between 21 January 2013 and 20 April 2013. The model was evaluated and described in terms of three parameters: structure, process and outcome. The description and assessment of each parameter was performed as follows: (I) Structure: profile of patients, pharmacist's professional profile, physical facility, informational material; (II) Process: drug-related problems, pharmaceutical interventions performed, results of pharmaceutical interventions; (III) Outcome: patient knowledge of medications. Dispensing service improved patient...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community Pharmacy; Medicines/dispensing; Patients/directive counseling; Medication/errors; Pharmaceutical Care; Quasi-experimental study.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100017

Mouse cell culture: methods and protocols
Provedor de dados: 60
Autores: Shinohara,Elvira M. Guerra.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400025

Protein synthesis regulation by leucine
Provedor de dados: 60
Autores: Vianna,Daiana; Teodoro,Gabriela Fullin Resende; Torres-Leal,Francisco Leonardo; Tirapegui,Julio.
In vivo and in vitro studies have demonstrated that high protein diets affect both protein synthesis and regulation of several cellular processes. The role of amino acids as substrate for protein synthesis has been established in the literature. However, the mechanism by which these amino acids modulate transcription and regulate the mRNA translation via mTOR-dependent signaling pathway has yet to be fully determined. It has been verified that mTOR is a protein responsible for activating a cascade of biochemical intracellular events which result in the activation of the protein translation process. Of the aminoacids, leucine is the most effective in stimulating protein synthesis and reducing proteolysis. Therefore, it promotes a positive nitrogen balance,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leucine; Protein synthesis; Transcription Factors; MTOR; Translation Protein.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100004

A fully validated microbiological assay for daptomycin injection and comparison to HPLC method
Provedor de dados: 60
Autores: Christ,Ana Paula; Machado,Mariana Souto; Ribas,Karla Giacomelli; Schwarzbold,Alexandre Vargas; Silva,Cristiane de Bona da; Adams,Andréa Inês Horn.
abstract Daptomycin (DPT) was the first lipopeptide antibiotic available for commercialization. It is active against gram-positive bacteria, including resistant strains. This work aimed to develop and validate a turbidimetric microbiologic assay to determine daptomycin in an injectable form. A 3x3 design was employed, at concentrations of 1, 2 and 4.0 µg/mL. The microorganism test used was Staphylococcus aureus ATCC 6538p, and Antibiotic Medium 3 was used as the culture medium. Method validation demonstrated that the bioassay was linear (r=0.9995), precise (RSD=2.58%), accurate (recovery 100.48± 2.11%), and robust. Degradation kinetics was also performed in an alkaline medium, indicating that daptomycin degradation follows first order kinetics under these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daptomycin/determination; Daptomycin injection/quality control; Turbidimetric microbiological assay/quantitative analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400775

Passiflora mucronata leaves extracts obtained from different methodologies: a phytochemical study based on cytotoxic and apoptosis activities of triterpenes and phytosterols constituents
Provedor de dados: 60
Autores: Silva,Isabel Cristina Vieira da; Oliveira,Pollyana Felix de; Barbosa,Gleyce Moreno; Wessjohann,Ludger A.; Cardozo-Filho,Lucio; Holandino,Carla; Muzitano,Michelle Frazão; Leal,Ivana Correa Ramos.
Cancer is one of the most prevalent diseases worldwide and the natural products could be a source of bioactive compounds. Passiflora mucronata (PM) belongs to a very known vegetal genus, although, there are no studies about cytotoxic activity or isolated compounds. Different extracts from PM were obtained by liquid-liquid partition (P), Soxhlet (Sox) and supercritical fluid (SFE1-5) extraction techniques, being compared concerning their yields, chemical profile and cytotoxicity. The Sox extracts showed the highest yields (6.03%: hexane; 2.51%: dichloromethane) followed by SFE (from 4.34 to 1.63%) and partitions (1.06 and 2.26%). The hexane partition (HP) showed the best cytotoxic activity against K562 cell line (IC50 = 18.72 µg.mL-1). From HP, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cytotoxic; Passiflora mucronata; Stigmasterol; Triterpenes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100510

HPLC method development for the simultaneous analysis of amlodipine and valsartan in combined dosage forms and in vitro dissolution studies
Provedor de dados: 60
Autores: Çelebier,Mustafa; Kaynak,Mustafa Sinan; Altınöz,Sacide; Sahin,Selma.
A simple, rapid and reproducible HPLC method was developed for the simultaneous determination of amlodipine and valsartan in their combined dosage forms, and for drug dissolution studies. A C18 column (ODS 2, 10 μm, 200 x 4.6 mm) and a mobile phase of phosphate buffer (pH 3.6 , 0.01 mol L-1):acetonitrile: methanol (46:44:10 v/v/v) mixture were used for separation and quantification. Analyses were run at a flow-rate of 1 mL min-1 and at ambient temperature. The injection volume was 20 μL and the ultraviolet detector was set at 240 nm. Under these conditions, amlodipine and valsartan were eluted at 7.1 min and 3.4 min, respectively. Total run time was shorter than 9 min. The developed method was validated according to the literature and found to be linear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amlodipine besylate; Valsartan; High Performance Liquid Chromatography; Method Development; Validation; Dissolution Study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400018

Nutritional composition of rice bran submitted to different stabilization procedures
Provedor de dados: 60
Autores: Faria,Simone Aparecida dos Santos Conceição; Bassinello,Priscila Zaczuk; Penteado,Marilene de Vuono Camargo.
In order to inactivate enzymatic deterioration, whole rice bran samples were subjected to two stabilization methods. Changes in nutritional value in terms of, concerning chemical composition, minerals and fatty acid content, were evaluated to supplement existing data and promote the utilization of rice bran in the human diet. The following homemade heat treatments were applied: roasting on a conventional stove or heating in a microwave oven. Based on the results, the different heating methods affected sample composition, since the levels of some nutrients of treated samples showed significant changes (p&lt;0.05) compared to corresponding raw samples. The rice bran treated on a conventional stove produced products with lower moisture (5.14±0.10 g/100 g) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rice bran/nutritional composition; Rice bran/nutritional value; Rice bran/heat treatment; Oryza sativa/phytochemistry; Human diet; Fatty acids/in food; Minerals/in food; Enzymatic inactivation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400008

Forced degradation of gliquidone and development of validated stability-indicating HPLC and TLC methods
Provedor de dados: 60
Autores: El-ghobashy,Mohamed Refaat; Yehia,Ali Mohamed; Helmy,Aya Helmy; Youssef,Nadia Fayek.
Forced degradation studies of gliquidone were conducted under different stress conditions. Three degradates were observed upon using HPLC and TLC and elucidated by LC-MS and IR. HPLC method was performed on C18 column using methanol-water (85:15 v/v) pH 3.5 as a mobile phase with isocratic mode at 1 mL.min-1 and detection at 225 nm. HPLC analysis was applied in range of 0.5-20 µg.mL-1 (r =1) with limit of detection (LOD) 0.177 µg.mL-1. TLC method was based on the separation of gliquidone from degradation products on silica gel TLC F254 plates using chloroform-cyclohexane-glacial acetic acid (6:3:1v/v) as a developing system with relative retardation 1.15±0.01. Densitometric measurements were achieved in range of 2 -20 µg /band at 254 nm (r = 0.9999) with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gliquidone/forced degradation/stability-indicating; HPLC/method validation; TLC/method validation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300603

Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer
Provedor de dados: 60
Autores: Bhalekar,Mangesh; Sonawane,Savita; Shimpi,Shamkant.
The aim of this study was to improve the mucoadhesive potential of xyloglucan polymer by the covalent attachment of cysteine as thiol moiety. The parent polymer xyloglucan was chemically modified by introducing sulphydryl bearing compound L-cysteine HCl. Different batches of xyloglucan-cysteine conjugates were prepared at varying reaction pH (2-6) and evaluated for optimum thiol incorporation, disulphide group content, swelling behavior, rheological properties and mucoadhesive properties. The obtained conjugates characterized in vitro by quantification of immobilized thiol groups; showed maximum thiol incorporation on xyloglucan (7.67 ± 0.14 %) at pH 5. The disulphide group content was found maximum (2.83 ± 0.12) at pH 6. The water uptake at end of 4 h was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucoadhesion; Thiomer; Xyloglucan; L-cysteine HCl.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200010

Efficacy, safety and tolerability of using abatacept for the treatment of rheumatoid arthritis
Provedor de dados: 60
Autores: Venson,Rafael; Wiens,Astrid; Correr,Cassyano Januário; Pontarolo,Roberto.
The objective is to provide an update on the clinical efficacy, safety and tolerability of the use of abatacept for treating rheumatoid arthritis. A systematic review (up to June 2011) followed by meta-analyses was performed. Randomized controlled clinical trials comparing abatacept at a dose of 10 mg/kg with a placebo, both with concomitant methotrexate, were used. Only high- or moderate-quality studies were included. The efficacy was evaluated based on changes in the ACR, DAS and HAQ; safety was assessed based on serious adverse events, serious infections, malignancies and deaths; tolerability was evaluated based on the withdrawals due to adverse events, serious adverse events and lack of efficacy. All these parameters were evaluated within one year of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abatacept/efficacy; Rheumatoid arthritis/treatment; Meta-analyses.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400022

Development and in vitro evaluation of sustained release multiparticulate tablet of freely water soluble drug
Provedor de dados: 60
Autores: Pandit,Ashlesha Pravin; Shinde,Rajendra Dattatray.
Blends of aqueous dispersion of a hydrophobic and hydrophilic polymer, namely Surelease®: hydroxypropyl methylcellulose (Surelease®: HPMC E15) were used as coating materials to control the drug release from coated pellets of the highly water soluble drug metoprolol succinate. Varying the polymer blends, ranges of drug release patterns were obtained at pH 6.8. The present study dealt with diffusion of drug through plasticized Surelease®/ hydroxypropyl methylcellulose (HPMC E15) films prepared by coating of drug and polymers onto non-pareil seeds using the solution layering technique. The release of metoprolol succinate from coated pellets was decreased with increased coating load of polymer. The optimized formulation was obtained by 3² full factorial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pellets; Nonpareil seeds; Surelease®; HPMC E15; Coating; Solution layering technique; Cushning agent; Metoprolol succinate.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300009

Effects of Cinnamomum cassia extract on oxidative stress, immunreactivity of iNOS and impaired thoracic aortic reactivity induced by type II diabetes in rats
Provedor de dados: 60
Autores: Uslu,Gözde Atila; Gelen,Volkan; Uslu,Hamit; Özen,Hasan.
Type II diabetes is known to cause neuropathy, nephropathy and retinopathy. However, cardiovascular disorders associated with diabetes have been ignored. In traditional medicine, cinnamon (Cinnamomum cassia) bark has been used for its abilities to relieve fever, inflammation and chronic bronchitis. In the present study, the effect of Cinnamomum cassia extract (CN) on the thoracic aorta in an experimental type II diabetes model was investigated. In rats administered with nicotinamide + streptozotocin, significant endothelial dysfunction and oxidative stress were characterised by increased inducible nitric oxide synthase (iNOS) and decreased insulin/proinsulin levels. This impairment was prevented by administering 1000 mg/kg metformin or 500-1000-1500 mg/kg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type II diabetes; Oxidative stress; INOS; Thoracic aorta reactivity; Cinnamomum cassia/effects.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300628

Evaluation of the bioequivalence of two formulations containing the combination of 400 mg of acetaminophen (paracetamol), 4 mg of phenylephrine and 4 mg of chlorpheniramine in capsules: open-label, three-way crossover study, partially replicated in healthy volunteers of both sexes
Provedor de dados: 60
Autores: Santos,Alessandra Ferreira dos; Santos,Quevellin Alves dos; Correa,Carlos Eduardo Melo; Coelho,Edvaldo Capobiango.
This study was carried out in order to compare the relative bioavailability of two different formulations containing 400 mg of acetaminophen + 4 mg of phenylephrine hydrochloride + 4 mg of chlorpheniramine maleate, Test formulation (Cimegripe®) and Reference formulation (Resfenol®) in 84 healthy volunteers of both sexes under fasting conditions. The study was conducted in a single dose, randomized, open-label, crossover 3-way and partially replicated. The tolerability was evaluated by the monitoring of adverse events and vital signs, results of clinical and laboratory tests. Plasma concentrations were quantified by validated bioanalytical methods using the ultra-performance liquid chromatography coupled to tandem mass spectrometry. The Cmax, Tmax, AUC0-t,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetaminophen; Phenylephrine; Chlorpheniramine; Bioequivalence; Partially replicated crossover; Ultra-high performance liquid chromatography; Mass spectrometry.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100564

Intralesional corticosteroid injection as an effective treatment method for oral lesions: a meta-analysis
Provedor de dados: 60
Autores: Gholizadeh,Narges; Sadrzadeh-Afshar,Maryam-Sadat; Sheykhbahaei,Nafiseh.
Intralesional corticosteroid injection (ICSI) is known as one of the main methods used for treating a wide range of lesions. It also results in a high concentration of drugs at lesion sites, with minimal systemic absorption. Thus, this study aimed to provide a review of the intralesional corticosteroid injection (ICSI) indications in the treatment of oral lesions. To this end; relevant key words were searched in the databases of PubMed, Google Scholar, Scopus, ScienceDirect, and UpToDate in the present study. Accordingly, the results of a total number of 62 case reports or case series articles were used in this study and the positive therapeutic effects of intralesional corticosteroid injection (ICSI) in 23 common oral lesions were reported. The most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intralesional corticosteroid injection; Triamcinolone; Betamethasone; Oral lesions; Oral disorders; Steroid injection.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100701

Development and stability assessment of liquid paediatric formulations containing sildenafil citrate
Provedor de dados: 60
Autores: Roque,Fátima; Rama,Ana Cristina; Sousa,João José; Pina,Maria Eugénia.
The purpose of this study is to develop and improve oral liquids formulations of sildenafil citrate for paediatric use. Four different formulations were developed, which are as follows: two aqueous solutions of sildenafil citrate (2.5 mg/mL), with or without preservatives, and two other solutions of sildenafil in simple syrup (1.25 mg/mL), with or without preservatives. All of the formulations were physically, chemically and microbiologically stable for three months. The results of the stability studies allowed for the optimisation of formulations without preservatives due to their simplicity and their similar stable conditions when compared to the formulations containing antimicrobials. The shelf life of both formulations was three months; however, upon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical stability; Microbiological stability; Sildenafil citrate; Paediatric formulations.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200021

Application of quality by design approach in RP-HPLC method development for simultaneous estimation of saxagliptin and dapagliflozin in tablet dosage form
Provedor de dados: 60
Autores: Gundala,Aruna; Prasad,KVSRG; Koganti,Bharathi.
A simple, sensitive, precise, accurate and robust high performance liquid chromatographic method has been developed for simultaneous estimation of saxagliptin (SAXA) and dapagliflozin (DAPA) in pharmaceutical formulation. Design of experiments (DoE) was applied for multivariate optimization of the experimental conditions of RP-HPLC method. Risk assessment was performed to identify the critical method parameters. Three independent factors; mobile phase composition, flow rate and column temperature were used to design mathematical models. Central composite design (CCD) was used to study the response surface methodology and to study in depth the effects of these independent factors. Desirability function was used to simultaneously optimize the retention time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saxagliptin/pharmacology Dapagliflozin/pharmacology Quality by Design RP-HPLC/methods Optimization/methods Chromatography  High Pressure Liquid/methods Dosage Forms Tablets/administration &amp;amp; Dosage.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100564

Physicochemical characterization and in vitro dissolution behavior of olanzapine-mannitol solid dispersions
Provedor de dados: 60
Autores: Krishnamoorthy,Venkateskumar; Suchandrasen,; Prasad,Verma Priya Ranjan.
The objective of the present work is to study the dissolution behavior of olanzapine from its solid dispersions with mannitol. Solid dispersions were prepared by melt dispersion method and characterized by phase solubility studies, drug content and in vitro dissolution studies. The best releasing dispersions were selected from release data, dissolution parameters and their release profiles. Solid state characterization techniques like Fourier transform infrared (FT-IR) spectroscopy, X-ray diffractometry, differential scanning calorimetry, near-infrared and Raman spectroscopy were used to characterize the drug in selected dispersions. The dispersions were also evaluated by wettability studies and permeation studies. The results of phase solubility studies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olanzapine; Mannitol; Solid dispersions.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200008

Organic cocoa extract -loaded surfactant-based systems intended to skin bioadhesion
Provedor de dados: 60
Autores: Fonseca-Santos,Bruno; Funagoshi,Erica Emi; Oliveira,José Ricardo Soares de; Sacramento,Luis Vitor Silva do; Santos,André Gonzaga dos; Leonardi,Gislaine Ricci; Chorilli,Marlus.
ABSTRACT This study was to develop, characterize, and evaluate the physical-chemical stability, in vitro antioxidant activity and in vitro safety profile of liquid crystalline systems (LCS) and microemulsions (MEs) with and without organic cocoa (OC) extract. LCS stabilized by surfactant polyoxyethylene 20 cetyl ether, containing water and oleic acid were studied. LCS and MEs were characterized using polarized light microscopy, small angle X-ray scattering, rheology and in vitro bioadhesion, and were evaluated for a period of 30 days by visual aspects, centrifuge test, pH value and relative density. PLM and SAXS assays showed the presence of domains of MEs, cubic and hexagonal mesophasephases, varying the proportions of the components of the formulations;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liquid crystals/evaluation/physical-chemical stability; Microemulsions/evaluation; Surfactant-based system; Organic cocoa extract; Skin bioadhesion.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300609

Misoprostol and teratogenesis in neonates
Provedor de dados: 60
Autores: Silva,Mariana Beatriz Scabora da; Ferreira,Aline de Assis; Habr,Soraya Ferreira; Bernardi,Maria Martha.
This paper is focused in some aspects of maternal exposure to misoprostol during perinatal period, and the abortive and teratogenesis effects on the fetus. The causes of malformations were revised, taking into account chemical, physical and environmental factors as well as the interaction between them. There are evidences that the practice of abortion tripled in Southern and Northeastern Brazil in 15 years, and one of the most frequent forms of abortion is through the use of misoprostol. In Brazil, 1991, 288,700 women were hospitalized as a consequence of complications induced by abortion attempt with this medicine. This fact resulted in the ban of misoprostol across our country, by Decree 344/98 determined by the Health Ministry. The use of misoprostol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Perinatal toxicology; Misoprostol; Malformations; Moebius syndrome; Abortion; Cytotec®.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300005

Ethics and development
Provedor de dados: 60
Autores: Aranha,Leila Rangel de Carvalho.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200001

Antibiotic resistance protocols
Provedor de dados: 60
Autores: Mamizuka,Elsa Masae.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400028

A review of analytical methods for the determination of four new phosphodiesterase type 5 inhibitors in biological samples and pharmaceutical preparations
Provedor de dados: 60
Autores: Codevilla,Cristiane Franco; Castilhos,Tamara dos Santos; Bergold,Ana Maria.
The introduction of oral phosphodiesterase type 5 inhibitor therapy in 1998 revolutionized the treatment of erectile dysfunction. Erectile dysfunction is the most common sexual problem in men. It often has a profound effect on intimate relationships and quality of life. The analysis of pharmaceuticals is an important part of the drug development process as well as for routine analysis and quality control of commercial formulations. Whereas the determination of sildenafil citrate, vardenafil and tadalafil are well documented by a variety of methods, there are few publications about the determination of udenafil, lodenafil carbonate, mirodenafil and avanafil. The paper presents a brief review of the action mechanism, adverse effects, pharmacokinetics and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphodiesterase type 5 inhibitors/determination; Phosphodiesterase type 5 inhibitors/pharmaceutical preparations; Phosphodiesterase type 5 inhibitors/determination; Phosphodiesterase type 5 inhibitors/biological samples; Analytical methods; Erectile dysfunction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100002

A quantitative study of the influence of coprocessing of binders on the mechanical properties of paracetamol tablets
Provedor de dados: 60
Autores: Ogunjimi,Abayomi Tolulope; Alebiowu,Gbenga.
A 2³ factorial experimental design has been used to quantitatively study individual and interaction effects of the nature of binder (N), concentration of binder (C) and the applied pressure (P) on two mechanical properties, namely, tensile strength (TS) and brittle fracture index (BFI), of paracetamol tablets. The factorial design was also used to study the quantitative effects of coprocessing of binders on the mechanical properties. The results obtained from this study suggest that the nature (i.e. plastic/elastic) and ratio of binders coprocessed together alter the influence of C and P on TS and BFI.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coprocessing; Neem gum; Microcrystalline cellulose; Binder; Tensile strength; Brittle fracture index; Paracetamol.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200006

Prevalence and costs of hospitalizations for poisoning and accidental intoxication in Brazilian elderly
Provedor de dados: 60
Autores: Abreu,Marcelle Silva de; Ferreira,Silvandro Diego de Albuquerque; Ferreira,Larissa Pelágia de Lima; Toneo Júnior,José Ferreira; Maciel,Wamberto Vieira; Maciel,Shirley Suely Soares Veras.
A cross-sectional study of secondary data/information obtained from the Hospital Information System (HIS) spanning the years 2008 - 2009 was performed. The distribution of the main hospital admissions by gender, age, color/race, region and federal unit of residence, average expenditure and average length of hospital stay, year of hospitalization and mortality rates (MR) were studied. The data collected were tabulated by TabNet and keyed into Microsoft Excel 2007. It was verified that elderly males (54.3%), from 60 to 69 years old (50.6%), nonwhites (36.3%) and residents of Southeast and North regions of the country had the highest rates of hospitalization. Seniors were hospitalized for an average of 4.8 days, and the major causes were exposure to alcohol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hospital Information System; Elderly patients/hospitalization; Medicines/inappropriated use; Hospitalization/rates; Poisoning; Alcohol/use.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400007

Ethanol extract of Cissampelos sympodialis ameliorates lung tissue damage in streptozotocin-induced diabetic rats
Provedor de dados: 60
Autores: Medeiros,Tessio David de; Pereira,Annyelly Tavares; Silva,Flavio Santos da; Bortolin,Raul Hernandes; Taveira,Karinna Veríssimo Meira; Abreu,Bento João da Graça Azevedo; Rezende,Adriana Augusto de; Farias,Naisandra Bezerra da Silva; Barbosa Filho,José Maria; Medeiros,Karina Carla de Paula.
Diabetes Mellitus (DM) is a metabolic syndrome characterized by hyperglycemia. Chronic complications affect a number of organs, including the lungs. Cissampelos sympodialis Eichl (Menispermaceae) is a plant used to treat respiratory diseases. The aim of this study was to evaluate the effect of Cissampelos sympodialis extract (CSE) in lungs of diabetic rats. We used 30 Wistar rats divided into three groups: control group (CG), diabetic group (DG) and diabetic Cissampelos sympodialis treatment group (DTG). Diabetes was induced by streptozotocin (40 mg/kg i.v.). The CSE (400 mg/kg, po) was administered daily, during four weeks, beginning one week after the onset of DM. The treatment with CSE was not able to reduce blood glucose levels after streptozotocin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cissampelos sympodialis; Diabetes; Lung; Pancreatic islets; Lipid profile.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100505

Barriers to the provision of asthma services and perceived practice towards asthma management among urban community pharmacists in Selangor, Malaysia
Provedor de dados: 60
Autores: Akram,Waqas; Ijaz,Naeem; Ahmad,Hassan; Jamshaid,Muhammad; Ismail,Nahlah Elkudssiah.
This study aims to assess the perceived practice and barriers towards the provision of asthma management services among urban community pharmacists in Selangor, Malaysia. The study also highlights both pharmacist and patient-related barriers in asthma counseling. One hundred fifty urban community pharmacists in Selangor, Malaysia, were randomly selected and recruited for the present cross sectional baseline study. Previous studies have explored pharmacists’ perception on their roles in asthma management in different parts of the world. The data was collected through self-explanatory questionnaires (containing 47 items). The extracted data from the completed questionnaires were analyzed descriptively and inferentially using Statistical Package for Social...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Perceived practive; Treatment barriers; Community pharmacists; Disease management.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400614

Contrasting the crospovidones functionality as excipients for direct compression
Provedor de dados: 60
Autores: Ramírez,Daniel García; Robles,Leopoldo Villafuerte.
Specific values of technological properties of excipients allow the establishment of numerical parameters to define and compare their functionality. This study investigates the functionality of Polyplasdones XL and XL10. Parameters studied included tablet disintegration profiles, compactibility profiles and powder flow. The results allowed the establishment of quantitative surrogate functionalities of technological performance, such as absolute number, and as a value relative to the known microcrystalline cellulose type 102. Moreover, the establishment of an explicit functionality to improve the technological performance of two diluents and a model drug was investigated, as was setting up of these functionalities, as quantitative values, to determine the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Excipients/functionality; Polyplasdones XL/functionality; Polyplasdones XL10/functionality; Tablets/compactibility; Tablets/disintegration time; Tablets/powder flow; Amoxicillin; Magnesium stearate; Superdisintegrants.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100155

Molecular docking, synthesis and in vitro antimalarial evaluation of certain novel curcumin analogues
Provedor de dados: 60
Autores: Dohutia,Chandrajit; Chetia,Dipak; Gogoi,Kabita; Bhattacharyya,Dibya Ranjan; Sarma,Kishore.
ABSTRACT The receptor protein PfATP6 has been identified as the common target of artemisinin and curcumin. The work was initiated to assess the antimalarial activity of six curcumin derivatives based on their binding affinities and correlating the in silico docking outcome with in vitro antimalarial screening results. A ligand library of thirty two Knoevenagel condensates of curcumin were designed and docked against PfATP6 protein and six compounds with the best binding scores were synthesized and screened for their antimalarial activity against the sensitive 3D7 strain of Plasmodium falciparum. ADME/Tox, pharmacokinetic and pharmacodynamic profiles of the designed compounds were analyzed and reported. 4-FB was found to have similar binding energy to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin/synthesis; Curcumin/antimalarial activity; Curcumin/Knoevenagel condensates; PfATP6; Malaria; Docking; In vitro; ADME/Tox.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400603

Identifying probiotic characteristics of Lactobacillus crispatus isolated from the vagina
Provedor de dados: 60
Autores: Er,Sevda; Erim,Ümit Can; Koç,Fatma; Kıvanç,Merih.
Lactobacilli prevent overproduction of pathogenic microorganisms and contribute protecting vaginal microbiota. Many probiotic microorganisms are categorized as Lactic Acid Bacteria. In this study, it was aimed identifying probiotic characteristics of Lactobacillus crispatus isolated from the vagina of a healthy woman. For this purpose, lactic acid, hydrogen peroxide and proteolytic activity quantities and auto-aggregation, co-aggregation and hydrophobicity abilities of Lactobacillus crispatus, which has been isolated and identified by 16s rRNA sequence analysis, were determined. Additionally, bile salt and acid resistance, along with antibiotic susceptibility of Lactobacillus crispatus were analyzed by the end of 3 hours. Lactic acid, hydrogen peroxide and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus crispatus/probiotic; Vaginal flora; Lactic acid bacteria.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100525

Regulation of herbal medicines in Brazil: advances and perspectives
Provedor de dados: 60
Autores: Carvalho,Ana Cecília Bezerra; Perfeito,João Paulo Silvério; Costa e Silva,Leandro Viana; Ramalho,Lívia Santos; Marques,Robelma France de Oliveira; Silveira,Dâmaris.
The National Policy of Integrative and Complementary Practices (PNPIC) in the Brazilian Unified Health System (SUS), and The National Policy of Medicinal Plants and Herbal Medicines (PNPMF) were launched in 2006. Based on these, the Brazilian Health Surveillance Agency (ANVISA) re-edited rules related to herbal medicines such as the Guideline to herbal medicine registration (RDC 14/10), the Good Manufacture Practices Guideline (RDC 17/10) and the List of references to assess the safety and efficacy of herbal medicines (IN 05/10). The requisites to prove herbal medicine's safety and efficacy were updated. Therefore, this review aims at presenting and commenting these new rules.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbal medicines/Regulation; Herbal medicines/Quality control; Medicines/Good Manufacture Practices; Pharmaceutical industry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300004

In vivo mucoadhesive strength appraisal of gum Manilkara zapota
Provedor de dados: 60
Autores: Sudarshan,Singh; Sunil B,Bothara.
The mucilage (MMZ) extracted from the seeds of Manilkara zapota(Linn.) P. Royen syn. using maceration techniques was evaluated for mucoadhesive strength by various in vitro and in vivo methods. The result showed that mucoadhesive strength of seeds mucilage have comparable property toward natural and synthetic polymers such as Guar Gum and hydroxyl propyl methyl cellulose (HPMC E5LV) under the experimental conditions used in this study. Briefly, it could be concluded that the seed mucilage of Manilkara zapota can be used as a pharmaceutical excipient in oral mucoadhesive drug delivery systems. Further, it may be appropriate to study the changes in these properties after chemical modifications.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manilkara zapota/use/pharmaceutics; Manilkara zapota/evaluation/mucoadhesive strength; Drugs/mucoadhesives/oral delivery systems; Natural polymers; Pharmaceutical excipients.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300689

Anatomical characters of the medicinal leaf and stem of Gymnanthemum amygdalinum (Delile) Sch.Bip. ex Walp. (Asteraceae)
Provedor de dados: 60
Autores: Duarte,Márcia do Rocio; Silva,Ariane Gonçalves.
Gymnanthemum amygdalinum (Delile) Sch.Bip. ex Walp. (Asteraceae), better known by its former name Vernonia amygdalina Delile, is a small shrub used in folk medicine as an antipyretic, laxative, antimalarial and anthelmintic. Studies have demonstrated that different vegetal extracts possess antioxidant, antimicrobial and antiparasitic activities. Among the bioactive metabolites, there are sesquiterpene lactones, saponins, polyphenols and flavonoids. This study investigated the leaf and stem microscopic characters of G. amygdalinum, aiming to expand the knowledge on this medicinal species and indicate anatomical structures. Plant material was fixed and sectioned by freehand and using a microtome. The sections were either stained or underwent standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gymnanthemum amygdalinum/pharmacognosy; Gymnanthemum amygdalinum/microscopic characterization; Vernonia amygdalina/pharmacognosy; Vernonia amygdalina/microscopic characterization; Asteraceae/pharmacognosy; Medicinal plants.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400011

In vitro cytotoxicity of chemical preservatives on human fibroblast cells
Provedor de dados: 60
Autores: Spindola,Daniel Gonsales; Hinsberger,Andre; Antunes,Valéria Maria de Souza; Michelin,Luis Felipe Gomes; Bincoletto,Claudia; Oliveira,Carlos Rocha.
ABSTRACT Preservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MTT assay and propidium iodide staining (IMU&gt;methylparaben&gt;sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preservatives; Pharmaceutical/chemistry; Fibroblasts/cytotoxicity; Cell death/drug effects; Cosmetics/additives.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100603

Comparative study of analytical methods by direct and first-derivative UV spectrophotometry for evaluation of losartan potassium in capsules
Provedor de dados: 60
Autores: Bonfilio,Rudy; Favoretto,Lívia Botacini; Pereira,Gislaine Ribeiro; Azevedo,Roberta de Cássia Pimentel; Araújo,Magali Benjamim de.
Losartan potassium is an antihypertensive non-peptide agent, which exerts its action by specific blockade of angiotensin II receptors. The aim of the present study was the validation and application of analytical methods for the quality control of losartan potassium 50 mg in pharmaceutical capsules, using direct and first-derivative UV spectrophotometry. Based on losartan potassium spectrophotometric characteristics, a signal at 205 nm of the zero-order spectrum and a signal at 234 nm of the first-derivative spectrum, were found adequate for quantification. The results were used to compare these instrumental techniques. The linearity between the signals and concentrations of losartan potassium in the ranges of 3.0-7.0 mg L-1 and 6.0-14.0 mg L-1 for direct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Losartan potassium; Direct UV spectrophotometry; First-derivative UV spectrophotometry.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100017

Development and validation of highly selective method for the determination of imatinib mesylate and dexketoprofen trometamol combination in three different media
Provedor de dados: 60
Autores: Coban,Ozlem; Degim,Zelihagul.
Imatinib mesylate is a small molecule used in cancer therapy as a thyrosine kinase inhibitor. Dexketoprofen trometamol is a non-steroidal anti-inflammatory drug that has seen use in cancer therapy in combination with an anticancer drug to minimize tumor size and to reduce pain in patients. In the present study, imatinib mesylate and dexketoprofen trometamol were selected as potential model drugs to be used in combination. A new, simple and selective Ultra Performance Liquid Chromatography method was developed and validated to determine the drug substances in distilled water, in a pH 7.4 phosphate buffer and in Dulbecco’s Modified Eagle Medium. The proposed method was developed using a BEH C-18 column with isocratic elution. A mixture of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Imatinib mesylate; Dexketoprofen trometamol; Ultra Performance Liquid Chromatography; Dulbecco’s Modified Eagle Medium.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100575

Characteristics and thermodynamics of the interaction of 6-shogaol with human serum albumin as studied by isothermal titration calorimetry
Provedor de dados: 60
Autores: Feroz,Shevin Rizal; Malek,Sri Nurestri Abdul; Tayyab,Saad.
ABSTRACT The interaction between 6-shogaol, a pharmacologically active ginger constituent, and human serum albumin (HSA), the main in vivo drug transporter, was investigated using isothermal titration calorimetry (ITC). The value of the binding constant, Ka (5.02 ± 1.37 × 104 M−1) obtained for the 6-shogaol-HSA system suggested intermediate affinity. Analysis of the ITC data revealed feasibility of the binding reaction due to favorable enthalpy and entropy changes. The values of the thermodynamic parameters suggested involvement of van der Waals forces, hydrogen bonds and hydrophobic interactions in the 6-shogaol-HSA complex formation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 6-Shogaol; Human serum albumin/Isothermal titration calorimetry; Ligand-protein interaction.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300443

Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol
Provedor de dados: 60
Autores: Jordao Júnior,Alceu Afonso; Domenici,Fernanda Aparecida; Lataro,Renata Cristina; Portari,Guilherme Vannucchi; Vannucchi,Helio.
Changes in the metabolism of methionine can cause hyperhomocysteinemia, inducing a triad of atherosclerosis, hypertension, and increased oxidative stress. The generation of free radicals and oxidative damage to DNA is important in the liver damage caused by ethanol. In this study, the effect of methionine overload associated or otherwise with acute administration of ethanol on homocysteine values, damage to DNA, lipoperoxidation and vitamin E was evaluated. Thirty rats were divided into 3 groups: Group Ethanol 24 hours (EG24), Group Methionine 24 hours (MG24), and Group Methionine and Ethanol 24 hours (MEG24). TBARS, vitamin E, GS and, homocysteine values were determined and the Comet assay was carried out. Increased GSH, vitamin E and homocysteine levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methionine; Homocysteine; Free Radicals; Antioxidants; Ethanol/induced damage.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400014

Development and validation of a microbial counting method for mebendazole oral suspension
Provedor de dados: 60
Autores: Assis,Polyana Araújo de; Andrade,Severino Borba de; Oliveira,Clécia Maria Carvalho de; Araújo,Patrícia Menezes de; Grangeiro Júnior,Severino; Ramos,Selma Verônica Vieira.
Mebendazole is an important medicine used to treat helminth infections. These infections affect more than two billion people worldwide. The LAFEPE® (Recife-PE, Brazil) produces the drug mebendazole oral suspension that contains the preservatives methylparaben and propylparaben in its formulation. Drugs that have antimicrobial properties due to preservatives must undergo neutralization of these compounds to allow microbial count testing according to recommendations by the official compendia. In order to obtain a validated method for microbial counting and to ensure its safety and reliability within the pharmaceutical industry, validation of preservative neutralization and of the method for microbial counting was performed according to the USP 30 and PDA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mebendazole/oral suspension; Microbial count.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300013

Novel ion exchange resin-based combination drug-delivery system for treatment of gastro esophageal reflux diseases
Provedor de dados: 60
Autores: Bhalekar,Mangesh Ramesh; Kadam,Nitin Madhukar; Patil,Nilam Hindurao; Gawale,Nitin Somnath; Madgulkar,Ashwini.
The present study involves preparation and characterization of a combination tablet of ranitidine in immediate release form and domperidone in sustained release form, using ion exchange resins. Ranitidine lowers acid secretion, while domperidone release over a prolonged period improves gastric motility thus justifying this combination in gastro esophageal reflux diseases (GERD) and ensuring patient compliance. Drug loading was carried out by batch method &amp;amp; resinates were characterized using FTIR, XRPD. Resinates were formulated as a combination tablet and evaluated for tablet properties &amp;amp; in vitro drug release. Resinates provided sustained release of domperidone and immediate release of ranitidine. IR and X-ray studies indicate complexation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/delivery system; Ranitidine/tablets; Ranitidine/immediate release; Domperidone/tablets; Domperidone/sustained release; Tablets/delivery system; Ion exchange resins/use; Ion exchange resins/drugs delivery system; Fármacos/sistema de liberação.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200021

Antimicrobial activity and bioautographic study of antistaphylococcal components from Caesalpinia pyramidalis Tull.
Provedor de dados: 60
Autores: Saraiva,Antonio Marcos; Saraiva,Cristiane Lopes; Gonçalves,Admário Marques; Soares,Rogério Ribeiro; Mendes,Fabrício de Oliveira; Cordeiro,Risonildo Pereira; Xavier,Haroudo Satiro; Pisciottano,Maria Nelly Caetano.
The antimicrobial activity of dry methanol and ethyl acetate extracts for the leaves, bark of the stem, peel of the root, flower, fruit and seed of Caesalpinia pyramidalis Tull. (catingueira) was performed against seventeen isolates of Staphylococcus aureus MRSA multiresistant strains, which included two isolates of S. aureus MSSA and two ATCC strains. The antimicrobial activity was tested by the agar diffusion method and the Minimum Inhibitory Concentration (MIC) was determined. The dry methanol extract of the root showed good antimicrobial activity with a MIC of less than 0.5 mg.mL-1. The dry ethyl acetate extracts exhibited lower antimicrobial activity, which might be explained by solubility problems and less diffusion in the agar medium. Results of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caesalpinia pyramidalis/antimicrobial activity; Caesalpinia pyramidalis/bioautographic study; Caesalpinia pyramidalis/antistaphylococcal components; Staphylococcus aureus/multiresistant; Brazilian Epidemic Clone.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100016

Effect of pH on weakly acidic and basic model drugs and determination of their ex vivo transdermal permeation routes
Provedor de dados: 60
Autores: Akula,Pranitha; P.K.,Lakshmi.
ABSTRACT The aim of the present study was to investigate the effect of donor pH on the transdermal permeability of the model drugs across rat skin and also to determine the major route of transport of the drugs. Weakly acidic drugs (partition coefficient) ibuprofen (3.6), aceclofenac (3.9), glipizide (1.9) and weakly basic drugs olanzapine (3.6), telmisartan (6.0), and sildenafil citrate (1.9) were selected for the study. The ex vivo permeation studies of these drugs at different donor pH (pH - 1.2, 4, 5, 6.8, 7.4, and 8) using Franz diffusion cell (area, 7.54 cm2) has shown a pH-dependent permeability. Among these drugs the weakly acidic drugs has shown higher permeation rates compared to the weakly basic drugs. The permeability coefficient and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Donor pH/effects; Weakly acidic drugs; Weakly basic drugs; PH solubility; Distribution coefficient; Ionization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200628

Wound healing activity in rabbits and antimicrobial activity of Hibiscus hirtus ethanolic extract
Provedor de dados: 60
Autores: Ravishankar,Kakaraparthy; Kiranmayi,Garlanka Venkata Naga; Prasad,Yejella Rajendra; Devi,Lankadi.
Generally, Plants have immense potential in the wounds management and treatment.In Chinese herbology, Hibiscus plant is a potent herb and have a good medicinal values but not scientifically approached. The present study aims to investigate the wound healing and antimicrobial activity of ethanolic extract of Hibiscus hirtus Linn.(HH). Wound healing activity was carried out by excision, incision and burn wound models. Antimicrobial activity was determined by cup plate method. Healing rate was assessed from the rate of wound contraction, epithelialization rate, tensile strength, hexosamine and hydroxyproline content. From the obtained results, it was indicated that the wound contraction and increased tensile strength of Hibiscus hirtus extract exhibits potent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hibiscus hirtus/wound healing activity/antimicrobial activity; Medicinal plants.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400606

Development of alternative methods for the determination of raloxifene hydrochloride in tablet dosage form
Provedor de dados: 60
Autores: Salazar,Fernanda Rodrigues; Codevilla,Cristiane Franco; Meneghini,Leonardo; Bergold,Ana Maria.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raloxifene/determination in tablets; High performance liquid chromatography/quantitative analysis; UV spectrophotometric method/quantitative analysis; Micellar capillary electrophoresis/ quantitative analysis; Stability-indicating methods.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200349

Isolation and characterization of homoisoflavonoids from Scilla persica HAUSSKN
Provedor de dados: 60
Autores: Hafez-Ghoran,Salar; Ebrahimi,Pouneh; Mighani,Hossein; Saeidnia,Soudabeh.
abstract Medicinal plants have many traditional claims including the treatment of ailments of infectious origin. In the evaluation of traditional claims, scientific research is extremely important. In this study, five homoisoflavonoids named 3-(4'-hydroxybenzylidene)-5,7-dihydroxy-6-methoxychroman-4-one(Autumnalin),3-(4'-hydroxybenzyl)-5,7-dihydroxy-6-methoxychroman-4-one (3,9-dihydro-autumnalin), 3-(3',4'-dihydroxybenzyl)-5,8-dihydroxy-7-methoxychroman-4-one,3-(3',4'-dihydroxybenzylidene)-5,8-dihydroxy-7-methoxychroman-4-one and 3-(3',4'-dihydroxybenzylidene)-5,7-dihydroxy-6-methoxychroman-4-one, were isolated from the bulbs of the plant Scilla persica HAUSSKN. Their structures were established on the basis of extensive spectroscopic analyses such as NMR,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ScillapersicaHAUSSKN/phytochemistry; Homoisoflavonoids/isolation; Medicinal plants.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400949

Pharmaceutical care in transplant patients in a university hospital: pharmaceutical interventions
Provedor de dados: 60
Autores: Martins,Bruna Cristina Cardoso; Souza,Thalita Rodrigues de; Luna,Ângela Maria Pita Tavares; Fonteles,Marta Maria de França; Firmino,Paulo Yuri Milen; Fernandes,Paula Frassinetti Castelo Branco Camurça; Garcia,José Huygens Parente; Oliveira,Cláudia Maria Costa de; Néri,Eugenie Desirèe Rabelo.
A descriptive and prospective study was conducted on the pharmaceutical care in the post-transplant outpatient clinic of Hospital Universitario Walter Cantidio of Universidade Federal do Ceará (HUWC/UFC), in Fortaleza- Ceará in the period of April to October of 2011. The aim of the present study was to describe the pharmaceutical interventions performed in a Pharmaceutical Care service structured in the liver and kidney transplant outpatient clinic of an academic hospital. The Pharmaceutical interventions (PI) were classified according to Sabater et al.(2005), with significance based on Riba et al.(2000) and the Negative Outcomes associated with Medication (NOM) established at the Third Consensus of Granada. Statistical analyses were performed using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care; Pharmaceutical interventions; Liver transplant/pharmaceutical interventions; Kidney transplant/pharmaceutical interventions; Patient safety; Pharmacotherapeutic treatment/monitoring.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400005

Antiophidian sera sterility control: topics in perspective
Provedor de dados: 60
Autores: Santos,Paula Gomes; Santos,Dilvani Oliveira; Seródio,Lilia Ribeiro; Marinho,Rachel de Souza; Rodrigues,Carlos Rangel; Cabral,Lucio Mendes; Pádula,Marcelo de; Sousa,Valéria Pereira de; Castro,Helena Carla.
The aim of this work is to review the most important topics about the antiophidic sera sterility, including obtaining methods, sterilization procedures and clean room control using Vital Brazil Institute (VBI) as an example. Bibliographical research was performed through Medline, Lilacs, PubMed, ISI and the Fundação Oswaldo Cruz - RJ and VBI Libraries, from 1960 to 2009. The antiophidic sera for human use are immunobiologic products produced in Brazil by three national laboratories, including VBI. Due to the parenteral use, these products should be sterile and pyrogen-free, which demands the microbiological control during the whole fabrication process. The sterility and pyrogen tests are important steps to ensure the quality and safety of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiologic control; Antiophidic sera; Sterility test; Contaminants.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300004

Towards the cosmetic application of Passiflora coccinea (Aubl.): antioxidant activity and photo protective capacity of the methanolic and glycolic leaf extracts
Provedor de dados: 60
Autores: Silva,Gisláine Correa da; Salvador,Marcos José; Bottoli,Carla Beatriz Grespan.
The present study aimed at the evaluation of Passiflora coccinea (Aubl.) antioxidant and photo protective in vitro activities, looking forward to their application as antiaging or sunscreen agents in cosmetic formulations. Methanolic and glycolic leaf extracts were prepared by three methods: ultrasound assisted extraction (UAE, 30 min.), maceration at room temperature (72 h) and maceration at 30 ºC (72 h). The antioxidant activities of the extracts were measured by DPPH and ORAC-FL assays and they were incorporated into a cosmetic emulsion to have their sun protection factor (SPF) measured spectrophotometricaly. The antioxidant activity of the emulsions were measured by DPPH and ORAC as well. C-glycosyl-flavones were identified in the extracts by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora coccinea; Emulsion; Antioxidant; SPF; Isovitexin; HPLC-DAD.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100511

Comparative evaluation of pancreatic histopathology of rats treated with olanzapine, risperidone and streptozocin
Provedor de dados: 60
Autores: Shah,Rehmat; Subhan,Fazal; Sultan,Sayed Mohammad; Ali,Gowhar; Ullah,Ihsan; Ullah,Sami.
Olanzapine and risperidone are widely prescribed atypical antipsychotics used in the treatment of schizophrenia and various other psychiatric disorders. Both of these drugs have been extensively reported to cause Type 2 diabetes mellitus and pancreatitis, however, the mechanism of olanzapine and risperidone-induced toxicity has not been so far unveiled. We, therefore, compared the streptozocin-induced pancreatic damage with that of pancreas isolated from olanzapine and risperidone treated rats. It was noticed that fibrotic growth, necrosis and derangement of the pancreatic islet cells caused by streptozocin were more pronounced than olanzapine and risperidone.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histopathology/pancreas; Antipsychotic drugs/evaluation; Olanzapine; Risperidone.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300622

Gelatin-based microspheres crosslinked with glutaraldehyde and rutin oriented to cosmetics
Provedor de dados: 60
Autores: Graziola,Fabiana; Candido,Thalita Marcílio; Oliveira,Camila Areias de; Peres,Daniela D'Almeida; Issa,Michele Georges; Mota,Joana; Rosado,Catarina; Consiglieri,Vladi Olga; Kaneko,Telma Mary; Velasco,Maria Valéria Robles; Baby,André Rolim.
ABSTRACT Glutaraldehyde (GTA) has been extensively used as a gelatin crosslinking agent, however, new natural ones have been suggested as more biocompatible. Polyphenols are possible candidates and the flavonols, such as rutin (RUT), also exhibit potential synergism with sunscreens and antioxidant agents used in cosmetics. In this work, gelatin microspheres (M0) were obtained and crosslinked with GTA 10 mM (MG) or RUT 10 mM (MR), dissolved in acetone:NaOH 0,01M (70:30 v/v). MG exhibited crosslinking extent of 54.4%. Gelatin, M0, MG and MR did not elicit any signs of skin damage, regarding the formation of erythema, the barrier function disruption and negative interference in the stratum corneum hydration. Oily dispersions containing M0, MG or MR, isolated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protection solar/tests; Gelatin/microspheres/safety testing; Glutaraldehyde/safety testing; Flavonoids/Rutin/use in cosmetics; Natural products/use in cosmetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400603

Alternative methods in toxicity testing: the current approach
Provedor de dados: 60
Autores: Araújo,Gabrielle Luck de; Campos,Maria Augusta Amaral; Valente,Maria Anete Santana; Silva,Sarah Cristina Teixeira; França,Flávia Dayrell; Chaves,Miriam Martins; Tagliati,Carlos Alberto.
Alternative methods are being developed to reduce, refine, and replace (3Rs) animals used in experiments, aimed at protecting animal welfare. The present study reports alternative tests which are based on the principles of the 3Rs and the efforts made to validate these tests. In Europe, several methodologies have already been implemented, such as tests of irritability, cell viability, and phototoxicity as well as in vitro mathematical models together with the use of in silico tools. This is a complex process that spans from development to regulatory approval and subsequent adoption by various official entities. Within this regulatory framework is REACH, the European Community Regulation for chemicals and their safe use. In Brazil, the BraCVAM (Brazilian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxicology/alternative methods; Toxicological analysis/alternative methods; Toxicology/21st; Century; Animals/use in experiments.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100005

Evaluation of the effect of natural peptide 'Urocortin' on corticotrophin releasing factor (CRF) receptor expression in ND7/23 cells
Provedor de dados: 60
Autores: Mohiuddin,Omair Anwar; Biggs,Chris.
CRF receptors are involved in the stress management of the cells and are believed to have a cytoprotective role in the body. CRF receptors have been reported to be potential drug targets for the treatment of neurodegenerative disorders. The cell line used in the study is ND7/23 (mouse neuroblastoma and rat dorsal root ganglion neuron hybridoma). The aim of the study was to confirm the expression of CRF receptors in ND7/23 cells and to determine if urocortin (Ucn) can enhance the expression of CRF receptors. ND7/23 cells were cultured in RPMI 1640 media and cells grown after the second passage were used for the experiments. RNA was extracted from the cells and amplified by RT-PCR to confirm the presence of CRF receptors. The cells were then subjected to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urocortin; ND7/23 cells; Corticotrophin releasing factor; RT-PCR; Neuronal cells.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100233

Acute neuronal injury: the role of excitotoxic programmed cell death mechanisms
Provedor de dados: 60
Autores: Scavone,Cristóforo.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400032

Cross-sectional study of the dispensation of synthetic anorectic drugs in community pharmacies in the city of Cruz Alta - State of Rio Grande do Sul
Provedor de dados: 60
Autores: Navarini,Marcieli Maria; Deuschle,Viviane Cecilia Kessler Nunes; Deuschle,Regis Augusto Norbert.
Obesity is defined as the excess adipose tissue in the body. Drugs responsible for inhibiting the appetite are called anorectics or appetite suppressants. Sibutramine, fenproporex and amfepramone belongs to this class, and are capable of causing physical or psychological dependence. The aim of this study was to evaluate the frequency of prescriptions for appetite suppressants in community pharmacies at Cruz Alta, State of Rio Grande do Sul, Brazil. The sales of fenproporex, amfepramone and sibutramine in the months of September, October and November 2010 and April, May and June 2011 were compared. It was observed that the most commonly dispensed anorectic in the three community pharmacies analyzed was sibutramine. In the months of September, October and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/prescriptions; Drugs/marketing control; Obesity/treatment; Anorectics; Amphetamine.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400737

Treatment interruption of biological drugs and tofacitinib in rheumatoid arthritis: A systematic review of case reports
Provedor de dados: 60
Autores: Wiens,Astrid; Borba,Helena Hiemisch Lobo; Leonart,Letícia Paula; Tonin,Fernanda Stumpf; Steimbach,Laiza Maria; Araújo,Ariane Gonçalves Silva de; Piazza,Thais; Ferreira,Vinicius Lins; Pontarolo,Roberto.
The aim of this study was to evaluate interruption of treatment with biological drugs and tofacitinib due to adverse events in patients with rheumatoid arthritis. A systematic review was performed in the electronic databases MEDLINE, Cochrane, Scopus, CRD, IPA, Lilacs and Scielo. Case reports addressing interruption of treatment due to any adverse event related to abatacept (ABA), adalimumab (ADA), anakinra (ANA), certolizumab pegol (CER), etanercept (ETA), golimumab (GOL), infliximab (IFX), rituximab (RTX), secukinumab (SEC), tocilizumab (TCZ), tofacitinib (TOF) or ustekinumab (UST) in rheumatoid arthritis patients were evaluated. Baseline data, patient profile, previous and current treatments, cause of discontinuation and information on reintroduction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; Biological drugs/treatment interruption; Tofacitinib; Adverse events.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400617

Development of granules from Phyllanthus niruri spray-dried extract
Provedor de dados: 60
Autores: Souza,Tatiane Pereira de; Gómez-Amoza,José Luiz; Pacheco,Ramón Martínez; Petrovick,Pedro Ros.
The aim of this study was to develop granules from Phyllanthus niruri spray-dried extract using dry and wet granulation and to assess techniques to enable the production of granules with improved technological characteristics and yields. Granules were characterized by granulometry, reological parameters, compression and hygroscopic behavior. Independent of the granulation technique, technologically developed granules presented particle diameter, bulk and tapped densities and compressibility indexes suitable for a solid dosage form. The compression behavior showed plastic and fragmentary deformation for granules produced by the dry granulation technique and predominantly plastic deformation for wet granulation. Concerning the humidity sorption, the study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phyllantus nururi/pharmacotechnics; Granules/production; Spray-dried extract; Wet granulation; Dry granulation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400009

Design and preparation of a novel colon-targeted tablet of hydrocortisone
Provedor de dados: 60
Autores: Ren,Yachao; Jiang,Lei; Yang,Shuman; Gao,Sainan; Yu,Hui; Hu,Jie; Hu,Dandan; Mao,Wenbin; Peng,Haisheng; Zhou,Yulong.
ABSTRACT The objective of this research was to design a new colon-targeted drug delivery system based on chitosan. The properties of the films were studied to obtain useful information about the possible applications of composite films. The composite films were used in a bilayer system to investigate their feasibility as coating materials. Tensile strength, swelling degree, solubility, biodegradation degree, Fourier transform infrared spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Scanning electron microscope (SEM) investigations showed that the composite film was formed when chitosan and gelatin were jointly reacted jointly. The results showed that a 6:4 blend ratio was the optimal chitosan/gelatin blend ratio. In vitro drug release results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitosan/gelatin complex film/drugs coating; Chitosan/multilayer coating system/drugs coating; Drugs/delivery system/colon-targeted; Tablets/bilayer-coated.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200239

Applications of USP apparatus 3 in assessing the in vitro release of solid oral dosage forms
Provedor de dados: 60
Autores: Pezzini,Bianca Ramos; Issa,Michele Georges; Duque,Marcelo Dutra; Ferraz,Humberto Gomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid dosage forms/in vitro release; Solid dosage forms/dissolution; Apparatus 3/use/evaluation of solid dosage forms; Reciprocating cylinders.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200265

Stability-indicating HPLC method for determination of amiodarone hydrochloride and its impurities in tablets: a detailed forced degradation study
Provedor de dados: 60
Autores: Coelho,Ana Silva; Ribeiro,Igor Felipe Rodrigues; Lages,Eduardo Burgarelli.
Resumo Amiodarone hydrochloride is one of the most important drugs used to treat arrhythmias. The USP monograph for amiodarone hydrochloride describes an HPLC method for the quantification of seven impurities, however, this method shows problems that result in unresolved peaks. Moreover, there is no monograph for tablets in this compendium. Thus, a stability indicating HPLC method was developed for the determination of amiodarone, its known impurities and degradation products in tablets. A detailed forced degradation study was performed submitting amiodarone API, tablets and placebo to different stress conditions: acid and alkaline hydrolysis, oxidation, metal ions, heat, humidity, and light. Amiodarone hydrochloride API was susceptible to degradation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amiodarone hydrochloride; Impurities; Forced degradation; Stability indicating methods; High-performance liquid chromatography.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100552

Synthesis, characterization and biological activity of a gold(I) triazenide complex against chronic myeloid leukemia cells and biofilm producing microorganisms
Provedor de dados: 60
Autores: Nunes,Melise Silveira; Garzon,Litiérri Razia; Rampelotto,Roberta Filipini; Tizotti,Maísa Kräulich; Martini,Rosiéli; Locatelli,Aline; Barbosa,Marcelo; Hörner,Manfredo; Hörner,Rosmari.
ABSTRACT The enhancement of anti-leukemia therapy and the treatment of infections caused by multidrug-resistant pathogens are major challenges in healthcare. Although a large arsenal of drugs is available, many of these become ineffective, and as a result, the discovery of new active substances occurs. Notably, triazenes (TZCs) have been consolidated as a promising class of compounds, characterized by significant biological activity, especially antiproliferative and antimicrobial properties. The aim of this study is the synthesis and characterization of a new triazenide complex of gold (I), as well as the in vitro assessment of its antiproliferative activity against the K562 cell line (Chronic Myeloid Leukemia), and antibacterial activity against bacterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triazenes/synthesis/characterization/biological activity; TZC/Gold ion complexed; Chronic myeloid leukemia; Antibacterial activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400617

Oxcarbazepine: validation and application of an analytical method
Provedor de dados: 60
Autores: Enéas,Paula Cristina Rezende; Oliveira,Renata Barbosa de; Pianetti,Gerson Antônio.
Oxcarbazepine (OXC) is an important anticonvulsant and mood stabilizing drug. A pharmacopoeial monograph for OXC is not yet available and therefore the development and validation of a new analytical method for quantification of this drug is essential. In the present study, a UV spectrophotometric method for the determination of OXC was developed. The various parameters, such as linearity, precision, accuracy and specificity, were studied according to International Conference on Harmonization Guidelines. Batches of 150 mg OXC capsules were prepared and analyzed using the validated UV method. The formulations were also evaluated for parameters including drug-excipient compatibility, flowability, uniformity of weight, disintegration time, assay, uniformity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxcarbazepine/quantitative analysis; Anticonvulsants/quantitative analysis; UV spectrophotometric/method validation; Capsules/dissolution.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200013

Bioinformatics
Provedor de dados: 60
Autores: Santos,Sílvia Regina Cavani Jorge.
Tipo: Info:eu-repo/semantics/review
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100024

Dentistry
Provedor de dados: 60
Autores: Fock,Ricardo Ambrósio.
Tipo: Info:eu-repo/semantics/review
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100025

Preparation and characterization of non-viral gene delivery systems with pEGFP-C1 Plasmid DNA
Provedor de dados: 60
Autores: Karagöz,Uğur; Kotmakçı,Mustafa; Akbaba,Hasan; Çetintaş,Vildan Bozok; Kantarcı,Gülten.
ABSTRACT In recent years, non-viral delivery systems for plasmid DNA have become particularly important. They can overcome the disadvantages of viral systems such as insertional mutagenesis and unpredicted immunogenicity. Some additional advantages of non-viral gene delivery systems are; good stability, low cost, targetability, delivery of a high amount of genetic materials. The aim of the study was to develop novel non-viral nanosystems suitable for gene delivery. Two formulations were developed for this purpose: water-in-oil microemulsion (ME) and solid lipid nanoparticles (SLN). The microemulsion was composed of Peceol, Tween 80, Plurol oleique, ethanol and water. The SLN was consisting of Precirol, Esterquat-1 (EQ1), Tween 80, Lecithin, ethanol and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microemulsion; Solid lipid nanoparticle; DNA delivery; Transfection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100608

6 weeks consumption of pure fresh coconut milk caused up-regulation of eNOS and CSE protein expression in middle-aged male rats
Provedor de dados: 60
Autores: Jansakul,Chaweewan; Naphatthalung,Jomkarn; Pradab,Sakda; Yorsin,Somruedee; Kanokwiroon,Kanyanatt.
Coconut milk (CCM) has been an important cooking ingredient in the Asia-Pacific region since ancient time. Due to its high content of saturated fatty acids, it has been considered atherogenic. We have tested if chronic consumption of fresh coconut milk by middle-aged male rat affects vascular function, plasma glucose and lipid profiles. Compared to control, CCM caused lower maximal contraction to phenylephrine of thoracic aortic rings and increased relaxation to acetylcholine that was abolished by N G-nitro-L-arginine (L-NA) or disruption of the endothelium. DL-propargylglycine caused slight increase in baseline tension of L-NA treated aortic rings of CCM-treated rats and produced higher contractile response of the aortic rings to low concentrations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coconut milk; NO. H2S; Plasma glucose; Thoracic aorta.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300608

Creatine supplementation: effects on blood creatine kinase activity responses to resistance exercise and creatine kinase activity measurement
Provedor de dados: 60
Autores: Machado,Marco; Pereira,Rafael; Sampaio-Jorge,Felipe; Knifis,Franz; Hackney,Anthony.
The purpose of this study was to determine the effects of creatine supplementation and exercise on the integrity of muscle fiber, as well as the effect of the supplementation on the creatine kinase (CK) assay measurement. Forty-nine sedentary individuals participated in a double-blind study and were divided into two groups: C (n=26) received 4x5-day packages of 0.6 g.kg-1 of body weight contained 50% of creatine + 50% of dextrose, and P (n=23) received packages containing only dextrose. On the first day the groups performed a 1RM test for bench press, seated row, leg extension, leg curl and leg press. On D7 they received the supplements. On the fourteenth day, they performed a training session of five exercises, each in three sets of ten repetitions at 75%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical exercise/effects; Creatine/supplementation/effects; Creatine Kinase/assay; Muscle fibers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400020

Study on influence of polymer and surfactant on in vitro performance of biodegradable aqueous-core nanocapsules of tenofovirdisoproxil fumarate by response surface methodology
Provedor de dados: 60
Autores: Srikar,Grandhi; Rani,Avula Prameela.
The major objective of this study was to investigate the effect of biodegradable polymer type and surfactant concentration on various characteristics viz. particle size, entrapment efficiency and drug release rate constant of aqueous core nanocapsules (ACNs) containing tenofovirdisoproxil fumarate. In this study, the nanocapsules were prepared by modified multiple emulsion technique with biodegradable polymers viz. poly(lactide-co-glycolide) of two different grades (PLGA RG502H and PLGA RG503H) and poly lactic acid (PLA R203H); and the surfactant employed was span 80. The experiments were designed under response surface methodology by employing the Design Expert software. Entrapment efficiency, particle size and drug release rate constant were taken as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aqueous core nanocapsules; Type of polymer; Surfactant concentration; Response surface methodology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100515

Dendrimers: from the art of building precise molecules to the dissemination through the world
Provedor de dados: 60
Autores: Vargas,Jeanine Giarolla.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700001

Ethnopharmacological activity of Hedera nepalensis K. Koch extracts and lupeol against alloxan-induced type I diabetes
Provedor de dados: 60
Autores: Hashmi,Waleed Javed; Ismail,Hammad; Jafri,Laila; Mirza,Bushra.
In this study, we investigated the protective effects of Hedera nepalensis crude extract, its fractions and lupeol in alloxan-induced diabetic rats. Lupeol and n-hexane (HNN) fraction significantly reduced the blood glucose level by increasing insulin level in time dependent manner, and also significantly increased amylase and lipase activity in diabetic rats. Elevated levels of alanine transaminases (ALT), aspartate transaminases (AST), thiobarbituric acid reactive substances (TBARS), nitrite, hydrogen peroxide (H2O2), total bilirubin and total protein in blood serum were efficiently restored to normal levels. Suppressed enzymatic activity of catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and peroxidase (POD) were also restored to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Amylase; Diabetes mellitus; Insulin; Hedera nepalensis; Lupeol.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100597

Pharmacoepidemiological profile and polypharmacy indicators in elderly outpatients
Provedor de dados: 60
Autores: Baldoni,André de Oliveira; Ayres,Lorena Rocha; Martinez,Edson Zangiacomi; Dewulf,Nathalie de Lourdes Sousa; Santos,Vânia dos; Obreli-Neto,Paulo Roque; Pereira,Leonardo Régis Leira.
This cross-sectional study was carried out with 1000 elderly outpatients assisted by a Basic Health District Unit (UBDS) from the Brazilian Public Health System (SUS) in the municipality of Ribeirão Preto. We analyzed the clinical, socioeconomic and pharmacoepidemiological profile of the elderly patients in order to identify factors associated with polypharmacy amongst this population. We used a truncated negative binomial model to examine the association of polypharmacy with the independent variables of the study. The software SAS was used for the statistical analysis and the significance level adopted was 0.05. The most prevalent drugs were those for the cardiovascular system (83.4%). There was a mean use of seven drugs per patient and 47.9% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacoepidemiology; Elderly/use of drugs; Polypharmacy; Drugs/rational use; Public Health System.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300006

Preparation, characterization and evaluation of moisturizing and UV protecting effects of topical solid lipid nanoparticles
Provedor de dados: 60
Autores: Golmohammadzadeh,Shiva; Mokhtari,Mohsen; Jaafari,Mahmoud Reza.
Solid lipid nanoparticles (SLN) were recently proposed as carriers for various pharmaceutical and cosmetic actives. These lipid nanoparticles can act as moisturizers and physical sunscreens on their own. Therefore, the full potential of these carriers has yet to be determined. The present study was aimed to determine and compare moisturizing and UV-protecting effects of different solid lipid nanoparticles (SLN) prepared by different solid lipids including Glyceryl monostearate (GMS), Precirol® (P) and cetyl palmitate (CP) as carrier systems of moisturizers and sunscreens. The influence of the size and matrix crystallinity of the solid lipids on the occlusive factor, skin hydration and UV-protection were evaluated by in vitro and in vivo methods. The SLN...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid lipid nanoparticles/moisturizing effects; Solid lipid nanoparticles/UV protecting.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400012

Biological and physicochemical stability of ceftazidime and aminophylline on glucose parenteral solution
Provedor de dados: 60
Autores: Santos,Carolina Alves dos; Oliveira-Nascimento,Laura; Knirsch,Marcos Camargo; Stephano,Marco Antônio; Pessoa Júnior,Adalberto; Penna,Thereza Christina Vessoni.
Ceftazidime is a broad spectrum antibiotic administered mainly by the parenteral route, and it is especially effective against Pseudomonas aeruginosa. The period of time in which serum levels exceed the Minimum Inhibitory Concentration (MIC) is an important pharmacodynamic parameter for its efficacy. One of the forms to extend this period is to administer the antibiotic by continuous infusion, after prior dilution in a Parenteral Solution (PS). The present work assessed the stability of ceftazidime in 5% glucose PS for 24 hours, combined or not with aminophylline, through High Performance Liquid Chromatography (HPLC). The physicochemical evaluation was accompanied by in vitro antimicrobial activity compared MIC test in the 24-hour period. Escherichia coli...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug/interactions; Injectables; Parenteral solutions; Ceftazidime/stability; Aminophylline/stability; High Performance Liquid Chromatography/quantitative analysis; Minimum inhibitory concentration (MIC).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400013

Simultaneous determination of candesartan and hydrochlorothiazide in human plasma by LC-MS/MS
Provedor de dados: 60
Autores: Bonthu,Mohan Gandhi; Atmakuri,Lakshmana Rao; Jangala,Venkateswara Rao.
Abstract A simple, sensitive, rapid and highly efficient LC-MS/MS method was developed for the determination of Candesartan and Hydrochlorothiazide simultaneously in human plasma. The method employed Zorbax eclipse C18 (150 X 4.6 mm, 5µ) column using acetate buffer: acetonitrile (25:75%, v/v) as the mobile phase. The mobile phase flow rate is 1 mL/min which was delivered into the mass spectrometer electron spray ionization chamber. The Liquid/liquid extraction procedure was used in the method for the extraction of analytes. The chromatograph was attached to a negative ion mode tandem mass spectrometer and the method was validated for all the parameters as per the guidelines of US-FDA. The ions were detected in multiple reaction monitoring mode and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candesartan; Hydrochlorothiazide; LC-MS/MS; Method validation; Human plasma.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100625

Deregulated expression of A1, Bcl-2, Bcl-xL, and Mcl-1 antiapoptotic proteins and Bid, Bad, and Bax proapoptotic genes in polycythemia vera patients
Provedor de dados: 60
Autores: Gasparotto,Elainy Patricia Lino; Tognon,Raquel; Ferreira,Aline Fernanda; Oliveira,Gislane Lelis Vilela; Palma,Patrícia Vianna Bonini; Zanichelli,Maria Aparecida; Souto,Elizabeth Xisto; Velano,Carlos Eduardo Engel; Simões,Belinda Pinto; Carrara,Rita de Cassia Viu; Kashima,Simone; Covas,Dimas Tadeu; Castro,Fabíola Attie de; Souza,Ana Maria de.
Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycythemia vera; Gene mutation; Gene expression; Apoptosis; Bcl-2 family members.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400025

Antibacterial, anti fungal and E. coli DNA cleavage of Euphorbia prostrata and Pelargonium graveolens extract and their combination with novel nanoparticles
Provedor de dados: 60
Autores: Dashamiri,Somayeh; Ghaedi,Mehrorang; Naghiha,Reza; Salehi,Amin; Jannesar,Ramin.
As there are a lot of antibacterial and anti-fungal resistant pathogens, researchers attempt to substitute antimicrobial drugs with various medical plants and novel nanoparticles. The present study was conducted to characterize antimicrobial activities of Euphorbia prostrata and Pelargonium graveolens extract alone and in combination with Mn-Ni@Fe3O4-NPs &amp; Mn: Fe (OH)3-NPs on the DNA cleavage of E. coli and also Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Aspergillus oryzae, and Candida albicans. The effects of antimicrobial activities on above scenarios were evaluated using disc diffusion, MIC, MBC, and E. coli DNA electrophoresis methods. The results showed that the effects of antibacterial assay values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbia prostrata/extract/antimicrobial activities; Pelargonium graveolens/extract/antimicrobial activities E coli; Candida albicans; Nanoparticles.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400625

Preparation and characterisation of Dextran-70 hydrogel for controlled release of praziquantel
Provedor de dados: 60
Autores: Campos,Flávio dos Santos; Cassimiro,Douglas Lopes; Crespi,Marisa Spirandeli; Almeida,Adélia Emília; Gremião,Maria Palmira Daflon.
A hydrogel was developed from 70 kDa dextran (DEX-70) and praziquantel (PZQ) incorporated as a model drug. Biopharmaceutical properties, such as solubility and dissolution rate, were analysed in the design of the hydrogel. Furthermore, the hydrogel was also characterized by IR spectroscopy and DSC. Tests of the swelling rate showed that the hydrogel swelled slowly, albeit faster than the rate for the free polymer. In dissolution tests, the hydrogel released the drug slowly and continuously. This slow release was similar to that observed in the swelling tests and resulted in controlled release of the drug. Thus, this dextran is a suitable polymer for the development of hydrogels as vehicles for the controlled release of drugs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrogel/development; Hydrogel/biopharmaceutical properties; Dextran/hydrogel development; Praziquantel/controlled release; Hydrogel/swelling test.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100009

Coenzyme Q10 and its effects in the treatment of neurodegenerative diseases
Provedor de dados: 60
Autores: Santos,Graciela Cristina dos; Antunes,Lusânia Maria Greggi; Santos,Antonio Cardozo dos; Bianchi,Maria de Lourdes Pires.
According to clinical and pre-clinical studies, oxidative stress and its consequences may be the cause or, at least, a contributing factor, to a large number of neurodegenerative diseases. These diseases include common and debilitating disorders, characterized by progressive and irreversible loss of neurons in specific regions of the brain. The most common neurodegenerative diseases are Parkinson's disease, Huntington's disease, Alzheimer's disease and amyotrophic lateral sclerosis. Coenzyme Q10 (CoQ10) has been extensively studied since its discovery in 1957. It is a component of the electron transportation chain and participates in aerobic cellular respiration, generating energy in the form of adenosine triphosphate (ATP). The property of CoQ10 to act as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coenzyme Q10; Antioxidant; Oxidative stress; Neurodegenerative diseases.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400002

Use of Opuntia ficus-indica (L.) Mill extracts from Brazilian Caatinga as an alternative of natural moisturizer in cosmetic formulations
Provedor de dados: 60
Autores: Damasceno,Gabriel Azevedo de Brito; Silva,Rebeca Manuelle Alexandre da Costa; Fernandes,Júlia Morais; Ostrosky,Elissa Arantes; Langassner,Silvana Maria Zucolotto; Ferrari,Márcio.
ABSTRACT The aim of this work was the obtainment of Opuntia fícus-indica (L.) Mill extract for the development of cosmetic formulations and in vivo evaluation of its moisturizing effects. The formulations were tested for preliminary and accelerated stability. Organoleptic characteristics, pH values and rheological behavior were assessed. The evaluation of moisturizing efficacy of the emulsions formulated with 3.0% of Polyacrylamide (and) C13-14 Isoparaffin (and) Laureth-7 containing 1.0 and 3.0% of O. ficus-indica hydroglycolic extract (EHG001) was performed using the capacitance method (Corneometer(r)) and the transepidermal water loss - TEWL evaluation (Tewameter(r)). The emulsions formulated were stable, exhibiting pseudoplastic and thixotropic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Opuntia fícus indica/emulsion stability; Opuntia fícus indica/cosmetic efficacy; Skin/cosmetics/use/efficacy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300459

The influence of protein malnutrition on the production of GM-CSF and M-CSF by macrophages
Provedor de dados: 60
Autores: Oliveira,Dalila Cunha de; Hastreiter,Araceli Aparecida; Borelli,Primavera; Fock,Ricardo Ambrósio.
ABSTRACT It is well established that protein malnutrition (PM) impairs immune defenses and increases susceptibility to infection. Macrophages are cells that play a central role in innate immunity, constituting one of the first barriers against infections. Macrophages produce several soluble factors, including cytokines and growth factors, important to the immune response. Among those growth factors, granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF). GM-CSF and M-CSF are important to monocyte and macrophage development and stimulation of the immune response process. Knowing the importance of GM-CSF and M-CSF, we sought to investigate the influence of PM on macrophage production of these growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein malnutrition; Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF); Macrophage Colony-Stimulating Factor (M-CSF).
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300375

Simultaneous estimation of rosuvastatin and amlodipine in pharmaceutical formulations using stability indicating HPLC method
Provedor de dados: 60
Autores: Ashfaq,Muhammad; Akhtar,Tazeem; Mustafa,Ghulam; Danish,Muhammad; Razzaq,Syed Naeem; Nazar,Muhammad Faizan.
A viable cost-effective and isocratic approach employing C-18 column (250 mm × 4.6 mm, 5 µm) based HPLC has been utilized to separate and estimate the drugs, rosuvastatin, amlodipine and their stress induced degradation products, simultaneously in pharmaceutical formulations. Focused on ICH guideline parameters, the efficient separation of both drugs and their degradation products was achieved by optimizing a 30:70 (v/v) solvent mixture of acetonitrile and 0.1 M ammonium acetate buffer (pH 5) as mobile phase. The flow rate of the mobile phase was 1.5 mL/min and all the detections were carried out at 240 nm using UV detector. The method was linear in the concentration range of 1-200 µg/mL for rosuvastatin with 0.996 coefficient of determination value. For...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High Performance Liquid Chromatography/quantitative analysis; Rosuvastatin/quantitative determination; Amlodipine/quantitative determination; Pharmaceutical formulations/quantitative analyis; Acetonitrile; UV Detector.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300629

Anti-inflammatory and toxicity studies of atranorin extracted from Cladina kalbii Ahti in rodents
Provedor de dados: 60
Autores: Melo,Marcelia Garcez Dória de; Araújo,Adriano Antunes de Souza; Serafini,Mairim Russo; Carvalho,Larissa Feitosa; Bezerra,Marília Santos; Ramos,Cledison Santos; Bonjardim,Leonardo Rigoldi; Albuquerque-Júnior,Ricardo Luiz Cavalcanti; Lima,Julianeli Tolentino; Siqueira,Rosana Souza; Fortes,Vanessa Silveira; Fonseca,Maria José Vieira; Quintans-Júnior,Lucindo José.
Atranorin (ATR) is the main compound from the lichen Cladina kalbii Ahti, which grows in the arid regions of northeastern Brazil. This study was conducted to evaluate the anti-inflammatory and toxicological properties of ATR. To evaluate anti-inflammatory properties, paw edema was induced by injecting 0.1 mL of carrageenan into the subplantar region of the right hind paw of rats, and leukocyte migration was induced by injection of 500 µL of carrageenan into the peritoneal cavity of mice. In addition, we determined ATR cytotoxicity in L929 cells by MTT assay and acute (5 g/kg-single dose) and subchronic (50 mg/kg-30 days) toxicity tests in Wistar rats. The results showed that ATR (100 mg/kg and 200 mg/kg) exhibited significant anti-inflammatory activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cladina kalbii/pharmacognosy; Atranorin/anti-inflamatory activity; Atranorin/toxicity; Medicinal plants.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400024

Punica granatum L. protects mice against hexavalent chromium-induced genotoxicity
Provedor de dados: 60
Autores: Ávila,Renato Ivan de; Guerra,Meirielle Teixeira; Borges,Kamilla de Almeida Souza; Vieira,Marcelo de Sousa; Oliveira Júnior,Luiz Marcos de; Furtado,Humberto; Mota,Mariana Flavia da; Arruda,Andréa Fernandes; Valadares,Marize Campos.
This study investigated the chemoprotective effects of Punica granatum L. (Punicaceae) fruits alcoholic extract (PGE) on mice exposed to hexavalent chromium [Cr(VI)]. Animals were pretreated with PGE (25, 50 or 75 mg/kg/day) for 10 days and subsequently exposed to a sub-lethal dose of Cr(VI) (30 mg/kg). The frequency of micronucleated polychromatic erythrocytes in the bone marrow was investigated and the Cr(VI) levels were measured in the kidneys, liver and plasm. For the survival analysis, mice were previously treated with PGE for 10 days and exposed to a single lethal dose of Cr(VI) (50 mg/kg). Exposure to a sub-lethal dose of Cr(VI) induced a significant increase in the frequency of micronucleated cells. However, the prophylactic treatment with PGE led...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemoprevention; Hexavalent chromium/induced genotoxicity; Oxidative damage; Pomegranate/chemoprotective effects; Punica granatum/chemoprotective effects; Punica granatum/pharmacognosy; Punicaceae/chemoprotective effects; Punicaceae/pharmacognosy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400008

Interchangeability among therapeutic equivalents of lamotrigine: evaluation of quality of life
Provedor de dados: 60
Autores: Girolineto,Beatriz Maria Pereira; Junior,Veriano Alexandre; Sakamoto,Américo Ceiki; Pereira,Leonardo Régis Leira.
Epilepsy is the most common serious neurological disorder worldwide. Approximately 70% of patients with epilepsy have their seizures controlled by clinical and pharmacological treatment. This research evaluated the possible influence of interchangeability among therapeutic equivalents of LTG on the clinical condition and quality of life of refractory epileptic patients. The study was divided into three periods of 42 days, and an equivalent therapeutic LTG randomly dispensed for each period (two similars - formulations A and B, and the reference product - formulation C). The mean dose of LTG was 5.5 mg/kg/day. The presence of side effects tends to have a greater deleterious effect on quality of life of refractory epileptics compared to variations in number...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy/treatment; Lamotrigine/therapeutic equivalents; Drugs/interchangeability.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100011

Pharmacovigilance of thalidomide in the Brazilian Health System and patient safety
Provedor de dados: 60
Autores: Drummond,Paula Lana de Miranda; Santos,Roberta Márcia Marques dos; Silva,Cristine de Araújo; Pádua,Cristiane Aparecida Menezes de.
In Brazil, thalidomide is manufactured by a public laboratory, distributed by the Unified Health System (SUS), and regulated by the National Health Surveillance Agency (Anvisa). Despite the concerns regarding the adverse effects of thalidomide, few drug utilization studies have been conducted to describe processes and outcomes related to this drug. The aim of this study was to elucidate the issues related to the utilization and control of thalidomide, and patient safety within the scope of SUS. In this cross-sectional study, we evaluated the articulation between an outpatient dermatology service of a referral hospital in infectology, the manufacturer, and Anvisa. Four data sources were used: i) interviews with health professionals; ii) data from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thalidomide; Patient safety; Notification; Pharmacovigilance.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100629

Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning: a study of antimicrobial activity
Provedor de dados: 60
Autores: Segala,Karen; Nista,Silvia Vaz Guerra; Cordi,Lívia; Bizarria,Maria Trindade Marques; Ávila Júnior,José de; Kleinubing,Sirlene Adriana; Cruz,Deborah Cristina; Brocchi,Marcelo; Lona,Liliane Maria Ferrareso; Caballero,Nelson Eduardo Durán; Mei,Lucia Helena Innocentini.
abstract This study examines the antimicrobial activity of silver nanoparticles incorporated into nanostructured membranes made of cellulose acetate (CA) and blends of chitosan/poly-(ethylene oxide, CTS/PEO) and prepared by electrospinning. The formation of chemically synthesized Ag nanoparticles (AgNPs) was monitored by UV-visible spectroscopy (UV-Vis) and characterized by transmission electron microscopy (TEM). The size distribution of the AgNPs was measured by dynamic light scattering (DLS), with an average size of approximately 20 nm. The presence of AgNPs on the surface of electrospun nanofibers was observed by field emission electron microscopy (FEG) and confirmed by TEM. The antimicrobial activity of AgNPs incorporated into nanostructured membranes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrospinning; Silver nanoparticles/antibacterial activity; Cellulose acetate; Chitosan.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400911

Nonsteroidal anti-inflammatory drugs as potential ecto-nucleotide phosphodiesterase inhibitors
Provedor de dados: 60
Autores: Tasneem,Shumaila; Saleem,Muhammad; Saeed,Sheikh Arshad.
Phosphodiesterases (PDE) are group of enzymes which catalyze the hydrolysis of cAMP and cGMP. Since these cyclic phosphate moieties worked as intracellular second messengers in numerous physiological processes, their inhibition can affect normal physiology of living system. NSAIDs are among the frequently prescribed medications, because of their efficacy as analgesic, antipyretic and anti-inflammatory agents. They are known to block cyclooxygenase pathway. In limited data NSAIDs has been shown anti-tumor potential, and phosphodiesterase inhibition has assumed to be one of the mechanism. To date no further evaluation being done. Further, NSAIDs are classified as cyclooxygenase inhibitors and phosphodiesterase inhibition can imprint its side effects. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-steroidal antiinflammatory drugs; Cyclooxygenase; Phosphodiesterase; Inflammation; CAMP.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100531

The medicine selection process in four large university hospitals in Brazil: Does the DTC have a role?
Provedor de dados: 60
Autores: Lima-Dellamora,Elisangela da Costa; Caetano,Rosangela; Osorio-de-Castro,Claudia Garcia Serpa.
Knowledge about evidence-based medicine selection and the role of the Drug and Therapeutics Committee (DTC) is an important topic in the literature but is scarcely discussed in Brazil. Our objective, using a qualitative design, was to analyze the medicine selection process performed in four large university hospitals in the state of Rio de Janeiro. Information was collected from documents, interviews with key informants and direct observations. Two dimensions were analyzed: the structural and organizational aspects of the selection process and the criteria and methods used in medicine selection. The findings showed that the DTC was active in two hospitals. The structure for decision-making was weak. DTC members had little experience in evidence-based...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/selection; Drug and Therapeutic Committees; University hospitals/medicines selection.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100173

Phytochemical constituents and preliminary toxicity evaluation of leaves from Rourea induta Planch. (Connaraceae)
Provedor de dados: 60
Autores: Kalegari,Milena; Miguel,Marilis Dallarmi; Dias,Josiane de Fátima Gaspari; Lordello,Ana Luísa Lacava; Lima,Cristina Peitz de; Miyazaki,Cristina Mayumi Sasaki; Zanin,Sandra Maria Warumby; Verdam,Maria Christina dos Santos; Miguel,Obdulio Gomes.
Most active plants are toxic at high doses and it is therefore important to investigate the preliminary toxicity of plant extracts. The Rourea induta species is a potential drug with no phytochemical or biological studies registered in the literature. Thus, a phytochemical study and a toxicity analysis of the ethanolic extract obtained from the leaves of Rourea induta Planch., Connaraceae, was run. A long chain hydrocarbon, n-tetracosane, and four flavonoids were identified: quercetin, and three glycosylated derivates, quercetin-3-O-α-arabinofuranoside, quercetin-3-O-β-xyloside and quercetin-3-O-β-galactoside. This is the first time these have been isolated in this species. The structures were elucidated by 13C NMR, ¹H NMR, UV and IR spectroscopy. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rourea induta/phytochemistry; Connaraceae/phytochemistry; Flavonoids; N-tetracosane; Plant extracts/toxicity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300023

Relation between CYP2C19 phenotype and genotype in a group of Brazilian volunteers
Provedor de dados: 60
Autores: Linden,Rafael; Ziulkoski,Ana Luiza; Tonello,Paula; Wingert,Maína; Souto,André Arigony.
The CYP2C19 gene presents polymorphism affecting the pharmacokinetics of several drugs of clinical importance. The purpose of this study was to investigate the correlation between CYP2C19 genotype and metabolic phenotype in a group of 38 Brazilian volunteers, evaluating the phenotype prediction capacity of the genotyping procedure. For CYP2C19 phenotyping, omeprazole was used as the probe drug, using the hydroxylation metabolic ratio as the phenotypic indicator. Venous blood samples were drawn before and three hours after an oral administration of 20 mg omeprazole. The plasma concentrations of omeprazole and hydroxy-omeprazole were determined by high performance liquid chromatography. The genotyping assay was carried out using a Real-Time-PCR-based assay,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic phenotyping; Metabolic genotyping; CYP2C19 gene.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300011

Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats
Provedor de dados: 60
Autores: Cao,Bianchuan; Li,Qiuju; Huang,Zhe; Huang,Xiufang; Zhu,Yihong; Xiao,Ke; Huang,Fuli; Zhong,Li.
T lymphocytes, cytokines, and macrophages play important roles in the clearance of Mycobacterium tuberculosis (Mtb) by the immune system. This study aimed to investigate the effects of isoniazid on the functions of both innate and adaptive immune cells. Healthy rats were randomly divided into experimental and control groups. Each group was randomly divided into three subgroups and named according to the duration of drug feeding, 1, 3, and 3 months followed by drug withdrawal for 1 month. The experimental groups were fed with isoniazid (12 mg/mL) and the control groups with normal saline. The percentage of CD4+ and CD8+T lymphocytes, level of interleukin (IL)-12 and interferon (IFN)-γ, and function of macrophages were determined at these three time points....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Immune; Isoniazid; Macrophages; T lymphocytes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100573

A pH-responsive drug delivery matrix from an interpolyelectrolyte complex: preparation and pharmacotechnical properties
Provedor de dados: 60
Autores: Montaña,Jorge Andrés; Perez,León Darío; Baena,Yolima.
ABSTRACT Interpolyelectrolyte complexes, which constitute a type of polymeric material obtained through the self-assembly of oppositely charged polymers, exhibit interesting properties for use in the design of smart matrices for drug delivery. In the present study, a stoichiometric interpolyelectrolyte complex (SIPEC) composed of Eudragit E® and Eudragit® L100 was obtained at pH 6.0 and characterized and evaluated as a hydrophilic matrix for dexibuprofen. The formation of a SIPEC was monitored by ζ-potential measurements and characterized using infrared spectroscopy, thermal analysis, and scanning electron microscopy. The results indicated that a SIPEC obtained under these conditions can be used as a matrix for controlling the release of dexibuprofen and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interpolyelectrolyte complex; Pharmacotechnical properties; Dexibuprofen; Controlled release; Hydrophillic matrix.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200620

Analysis of the presence of FLT3 gene mutation and association with prognostic factors in adult and pediatric acute leukemia patients
Provedor de dados: 60
Autores: Burnatt,Graciele; Licínio,Marley Aparecida; Gaspar,Pâmela Cristina; Ferreira,Arthur Schveitzer; Reis,Manoela Lira; Moraes,Ana Carolina Rabello de; Sincero,Thaís Cristine Marques; Santos-Silva,Maria Cláudia.
ABSTRACT When the FLT3 gene is mutated, it originates a modified receptor with structural changes, which give survival advantage and malignant hematopoietic cell proliferation. Thus, the presence of mutations in this gene is considered an unfavorable prognostic factor. A total of 85 consecutive samples of newly diagnosed untreated patients with AL were included in the study after they provided their informed consent. FLT3 gene mutations were detected by PCR. For the pediatric group, a positive correlation was observed between WBC count and the presence of FLT3-ITD in patients with AML and ALL. Furthermore, children with AML who had the FLT3-ITD mutation showed a tendency to express CD34 in blast cells. In the adult group, the AML patients with FLT3-ITD who...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FLT3-ITD/mutation; Acute leucemia/prognostic factors; Acute leucemia/adults patients; Acute leucemia/pediatric patients; AML; ALL; FLT3-D835.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200617

Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion
Provedor de dados: 60
Autores: Andréo,Marcio Adriano; Rufino,Iasmin Mimoto; Ubaldo,Daniela Cecilia Ortiz de Orue; Herbst,Estela Boaro; Silva,Heron Dominguez Torres da; Oliveira,Pedro Gonçalves de; Ferrari,Marcio; Rosa,Paulo Cesar Pires; Lopes,Patrícia Santos; Leite-Silva,Vânia Rodrigues.
ABSTRACT Aiming to alter and/or improve permeation of active compounds in the skin, many strategies have been developed, including biophysical methods. One of the physical absorption techniques, currently known as Cryo Laser Phoresis (CLP), consists of an apparatus that emits radiation on polar or nonpolar molecules of the active substance, resulting in faster penetration when in comparison to the standard topical application. The goal of this work was to evaluate the efficacy of a method that proposes to increase cutaneous permeation of diclofenac sodium by using CLP technique. The influence on permeation was evaluated ex vivo, using Franz cell and human skin obtained from cosmetic surgery. The results were evaluated using statistical methods and data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryo Laser Phoresis/dematologic use; Cutaneous permeation; Diclofenac sodium/cutaneous permeation; Franz cell.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100008

Bioisosteric modification on melatonin: synthesis of new naphthalene derivatives, in vitro antioxidant activity and cytotoxicity studies
Provedor de dados: 60
Autores: Shirinzadeh,Hanif; Ghalia,Mohammed; Tascioglu,Alev; Adjali,Ferial Intissar; Gunesacar,Gulsen; Gurer-Orhan,Hande; Suzen,Sibel.
Melatonin (MLT) is a strong free radical scavenger that protects the body from the deleterious effects of excess oxidants. Synthesis of MLT analogue compounds with antioxidant potency has recently attracted the interest of researchers. In general, the strategy consists of modifying the groups in the different sites of the indole ring or replacing the indole ring with an analogue. As part of our ongoing research, the antioxidant capacity and cytotoxicity of newly synthesized MLT analogue naphthalene derivatives were evaluated. The radical scavenging activity was tested by a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Most of the synthesized compounds showed significant antioxidant activity in comparison to MLT. The structure-activity relationship was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Cytotoxic; Melatonin; MTT; DPPH.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100550

Determination of nimodipine in plasma by HPLC-MS/MS and pharmacokinetic application
Provedor de dados: 60
Autores: Nascimento,Demétrius Fernandes do; Moraes,Manoel Odorico de; Bezerra,Fernando Antônio Frota; Pontes,Andréa Vieira; Uchoa,Célia Regina Amaral; Moraes,Renata Amaral de; Leite,Ismenia Osório; Santana,Gilmara Silva de Melo; Santana,Ana Paula Macedo; Leite,Ana Lourdes Almeida e Silva; Pedrazzoli Júnior,José; Moraes,Maria Elisabete Amaral de.
To develop and validate a rapid, specific and highly sensitive method to quantify nimodipine in human plasma using dibucaine as the internal standard (IS). The analyte and IS were extracted from plasma samples by liquid-liquid extraction using hexane-ethyl acetate (1:1 v/v). The chromatographic separation was performed on a Varian® Polaris C18 analytical column (3 μm, 50 x 2.0 mm) and pre-column SecurityguardTM C18 (4.0 x 3.0 mm) with a mobile phase of Acetonitrile-Ammonium acetate 0.02 ml/L (80:20v/v). The method had a chromatographic run time of 4.5 min and linear calibration curve over the range of 0.1- 40 ng/mL (r &gt; 0.9938). The limit of quantification was 100 pg/mL. Acceptable precision and accuracy were obtained for concentrations over the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nimodipine/determination in plasma; Nimodipine/pharmacokinetic profile; Nimodipine/quantitative analysis; Pharmacokinetics/method validation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400008

Hypoglycemic activity of Capparis ovata desf. var. palaestina zoh. methanol extract
Provedor de dados: 60
Autores: Okur,Mehmet Evren; Özbek,Hanefi; Polat,Derya Çiçek; Yılmaz,Sezen; Arslan,Rana.
Caper (Capparis ovata Desf. and Capparis spinosa L.) is naturally widespread in Turkey. Traditionally, buds, fruits, seeds and roots of this plant are used as tonic, diuretic, anti-rheumatic, expectorant, antidiabetic, and antifungal. The aim of this study is to evaluate potential hypoglycemic effect of C. ovata var. palaestina extracts in alloxan-induced diabetic mice. For this purpose; diabetic mice were administered with 100, 300, 500 mg/kg (i.p.) doses of methanol extract of bud and fruit. Blood glucose levels were screened 60, 120, 240 and 360 min. after treatment. Furthermore, high resolution mass spectrometry (HRMS) analysis, ABTS and DPPH free radical scavenging activity test, and phenolic and flavonoid compounds analysis of extracts were carried...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. ovata var. palaestina/extract; C. ovata var; Palaestina/hypoglycemic effect/antioxidant effects; Diabetes mellitus; Mice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300630

Retinoic acid liposome-hydrogel: preparation, penetration through mouse skin and induction of F9 mouse teratocarcinoma stem cells differentiation
Provedor de dados: 60
Autores: Xia,Hongmei; Cheng,Yongfeng; Xu,Yinxiang; Cheng,Zhiqing.
Retinoic acid (RA), a metabolite of retinol, is one of the most biologically active forms of retinoid and plays vital roles in embryonic development and in the regulation of cell proliferation and differentiation. Knowing that liposomes simulate cell membranes and that hydrogel is an ideal delivery vehicle for topical medicine, liposome-hydrogel is a novel preparation that has synergistic advantages over each component separately. Our objective was to investigate the characteristics of RA liposome-hydrogel. For quality control of the RA-loaded liposomes, we measured their morphology, particle size, Zeta-potential, and entrapment efficiency. Then we determined the viscosity of RA liposome-hydrogel. Next, the diffusion through mouse skin was explored,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Retinoic acid (RA); RA liposome-hydrogel/characteristics; RA liposome-hydrogel/preparation; RA liposome-hydrogel/diffusion/experimental study; F9 cell/differentiation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300541

Relationship between glycemic control and OPG gene polymorphisms with lower bone mineral density in patients with type 1 Diabetes mellitus
Provedor de dados: 60
Autores: Loureiro,Melina Bezerra; Ururahy,Marcela Abbott Galvão; Souza,Karla Simone Costa de; Oliveira,Yonara Monique da Costa; Silva,Heglayne Pereira Vital da; Bortolin,Raul Hernandes; Bezerra,João Felipe; Hirata,Rosario Dominguez Crespo; Maciel-Neto,José Jorge; Arrais,Ricardo Fernando; Almeida,Maria das Graças; Hirata,Mario Hiroyuki; Rezende,Adriana Augusto de.
ABSTRACT The aim of the present study was to investigate the bone mineral density (BMD) of patients with type 1 Diabetes mellitus (T1DM). We also assessed the association between osteoprotegerin (OPG) genetic polymorphisms and BMD. Genotyping was performed for 1181G&gt;C and 163A&gt;G OPG polymorphisms by allelic discrimination in 119 patients with T1DM and 161 normoglycemic (NG) individuals, aged 6 to 20 years old. Glycemic control, serum parameters of bone metabolism and BMD were evaluated. T1DM patients showed low BMD, poor glycemic control and decreased total calcium values when compared to controls (p &lt; 0.05). For all the polymorphisms studied, the genotype and allele frequencies in patients with T1DM were not significantly different from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 1 Diabetes mellitus/bone biomarkers; Bone mineral density; OPG polymorphism/children and adolescents.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400616

A 2D-QSPR approach to predict blood-brain barrier penetration of drugs acting on the central nervous system
Provedor de dados: 60
Autores: Sá,Matheus Malta de; Pasqualoto,Kerly Fernanda Mesquita; Rangel-Yagui,Carlota de Oliveira.
Drugs acting on the central nervous system (CNS) have to cross the blood-brain barrier (BBB) in order to perform their pharmacological actions. Passive BBB diffusion can be partially expressed by the blood/brain partition coefficient (logBB). As the experimental evaluation of logBB is time and cost consuming, theoretical methods such as quantitative structure-property relationships (QSPR) can be useful to predict logBB values. In this study, a 2D-QSPR approach was applied to a set of 28 drugs acting on the CNS, using the logBB property as biological data. The best QSPR model [n = 21, r = 0.94 (r² = 0.88), s = 0.28, and Q² = 0.82] presented three molecular descriptors: calculated n-octanol/water partition coefficient (ClogP), polar surface area (PSA), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Two-dimensional quantitative structure-property relationships (2D-QSPR); Calculated n-octanol/water partition coefficient (ClogP); Blood-brain barrier; Benzodiazepines.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400016

Beneficial effects of methanolic extract of Indigofera linnaei Ali. on the inflammatory and nociceptive responses in rodent models
Provedor de dados: 60
Autores: Kumar,Raju Senthil; Rajkapoor,Balasubramanian; Perumal,Perumal; Kumar,Sekar Vinoth; Geetha,Arunachalam Suba.
ABSTRACT Indigofera linnaei Ali. (Tamil Name: Cheppu Nerinjil) belongs to the family Fabaceae, used for the treatment of various ailments in the traditional system of medicine. In the present study, the beneficial effects of methanol extract of whole plant of I. linnaei (MEIL) were evaluated on inflammation and nociception responses in rodent models. In vitro nitric oxide (NO), lipoxygenase (LOX) and cyclooxygense (COX) inhibitory activities were also performed to understand the mode of action. MEIL at the dose of 200 &amp; 400 mg/kg, p.o. significantly inhibited carrageenan induced rat paw volume and reduced the weight of granuloma in cotton pellet granuloma model. The results obtained were comparable with the standard drug aceclofenac. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IIndigofera linnaei/pharmacognosy; Indigofera linnaei/anti-inflammatory activity; Indigofera linnaei/analgesic activity; Indigofera linnaei/anti-nociceptive effect; Cyclooxygenase/inhibitors; Lipoxygenase/inhibitors; Medicinal plants.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100013

Antioxidant activity and physical-chemical properties of spray and spouted bed dried extracts of Bauhinia forficata
Provedor de dados: 60
Autores: Souza,Claudia Regina Fernandes; Georgetti,Sandra Regina; Salvador,Marcos José; Fonseca,Maria José Vieira; Oliveira,Wanderley Pereira.
Two distinct drying methods (spouted bed and spray drying) were used for production of dried extracts of Bauhinia forficata Link (Leguminosae, Caesalpinoideae). High-quality powder products in terms of physical and chemical properties were obtained. HPLC fingerprints revealed that the chromatographic profiles of flavonoid compounds present in the dried extract did not change significantly, due to drying. In general, the spouted bed drying caused a degradation of total flavonoids than was lower than that of the spray drying. Antioxidant properties of the dried extract, examined by their radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and inhibition of lipid peroxidation induced by Fe+2 assays (LPO), confirmed their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bauhinia forficata/antioxidant activity; Bauhinia forficata/dried extract; Bauhinia forficata/properties; Flavonoids/qualitative analysis; Spouted bed/utilization; Spray drying/utilization.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200004

Antioxidant and antibacterial activity of extracts, fractions and isolated substances from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don
Provedor de dados: 60
Autores: Andrade,Cláudia Alexandra de; Carvalho,João Luiz de Souza; Cunico,Miriam Machado; Lordello,Ana Luísa Lacava; Higaskino,Carmen Etsuko Kataoka; Almeida,Siumara Costa da Cruz; Dias,Josiane de Fátima Gaspari; Kerber,Vitor Alberto; Miguel,Marilis Dallarmi; Miguel,Obdúlio Gomes.
The extracts and fractions from the flowers of A. podalyriifolia were analyzed previously for antibacterial activity using diffusion in disk, Antioxidant properties were evaluated by determining radical scavenging power (DPPH test) and total phenol content was measured (Folin method). The present study describes the in vitro antibacterial (determining minimum inhibitory concentration) and antioxidant activities (by thiobarbituric acid reactive species - TBARS method) for the ethanol extract, dichloromethane and ethyl acetate fractions and two flavanones (naringenin and 5-β-D-glycosyl-naringenin) isolated from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don. The flavanones naringenin and 5-β-D-glycosyl-naringenin had not previously been obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acacia podalyriifolia/qualitative analysis; Acacia podalyriifolia/phytochemistry; Acacia podalyriifolia/antibacterial activity; Acacia podalyriifolia/antioxidant properties; Flavanones; 5-β-D-glycosyl-naringenin; Naringenin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400013

Tretinoin-based formulations - influence of concentration and vehicles on skin penetration
Provedor de dados: 60
Autores: Bagatin,Edileia; Wagemaker,Tais Aleriana Lucon; Aguiar Júnior,Nelson dos Reis; Gianeti,Mirela Donato; Gonçalves,Erika Maria Berardo; Campos,Patrícia Maria Berardo Gonçalves Maia.
Tretinoin is used in the management of acne and it is part of a gold standard treatment for photoaging. It has also been reported as an agent for superficial chemical peeling in highly concentrated formulations with few considerations about skin penetration. The aim of this study was to evaluate the influence of drug concentration and vehicles currently used on skin penetration of tretinoin. In vitro permeation tests were carried out using Franz diffusion cells fitted with porcine ear skin and 10% aqueous methanol in the receptor compartment. Formulations studied, cream or hydroalcoholic dispersion, containing 0.25%, 1% and 5% of tretinoin were placed in the donor compartment for six hours. Tretinoin concentration in skin layers was measured by high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TretinoinTretinoin/skin penetration/in vitro study; Chemical peeling; Topical formulations/analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100085

Development of amphotericin b Based organogels against mucocutaneous fungal infections
Provedor de dados: 60
Autores: Gopalan,Kavya; Jose,Jobin.
Amphotericin B is a broad spectrum antifungal agent used to treat fungal infections. Organogel is a semisolid preparation in which the apolar phase gets immobilized within spaces of the three-dimensional structure. The current study aimed at the formulation and comparative evaluation of sorbitan monostearate organogels and pluronic lecithin organogels (PLO). Different compositions of span 60 based organogels were prepared by varying the concentrations of the span 60 and PLO gels were prepared by varying the concentration of Pluronic F 127. The developed organogels were subjected to various characteristics such as pH, viscosity, spreadability, extrudability, and drug release studies. The optimized formulations were evaluated against Candida albicans and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphotericin B; Organogel; Span 60; Pluronic F127; Lecithin.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100563

Effects of fat replacement on properties of whole wheat bread
Provedor de dados: 60
Autores: Scheuer,Patrícia Matos; Mattioni,Bruna; Barreto,Pedro Luiz Manique; Montenegro,Flávio Martins; Gomes-Ruffi,Cristiane Rodrigues; Biondi,Sílvia; Kilpp,Mariana; Francisco,Alicia de.
Bread characteristics are dependent upon the chemical composition and fiber content of the wheat flour used. A healthy diet can help prevent chronic diseases, where whole grains and fewer calories from fat are components of a healthy diet. The aims of this study were to determine the centesimal composition of wheat flour samples (whole, refined, and flour blends) along with their farinographic parameters (water absorption, development time and stability) and also to verify the specific volume, crumb texture profile and digital imaging of bread loaves made with 60% whole wheat flour and 3% fat or fat replacer. Increasing the whole wheat flour percentage in the blends also increased levels of protein, ash and insoluble fiber. All the farinographic parameters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat flour/centesimal composition; Whole wheat bread/characteristics; Fat/replacers; Food fibers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400703

Extract of Solidago chilensis Meyen inflorescences: cytotoxicity and inhibitory activity on nitric oxide synthesis in activated macrophage cell line J774A.1
Provedor de dados: 60
Autores: Brito,Thais Morais de; Amendoeira,Fabio Coelho; Oliveira,Temistocles Barroso de; Frutuoso,Valber da Silva; Ferraris,Fausto Klabund; Valverde,Simone Sacramento.
Solidago chilensis Meyen (= Solidago microglossa) popularly known as “Brazilian arnica” is used to treat of inflammatory disorders. S. chilensis is constant in the Therapeutic Memento of the Rio de Janeiro city and belongs to the medicinal species of Brazilian National List of Medicinal Plants of Interest of the Unified National Health System (SUS). There are no studies in the literature showing the direct activity of this plant species on immune system cells. The present study evaluated the chemical composition as well as the cytotoxic and pharmacological activity of the ether-ethanol extract from S. chilensis inflorescences (SCIE) in murine macrophage cell line J774A.1. The results showed that higher concentrations (50 to 200 µg/mL) of SCIE had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-inflammatory activity; Medicinal plant; Asteraceae; Diterpenes; Flavonoids.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100594

The anti-obesity potential of Ilex paraguariensis: results from a meta-analysis
Provedor de dados: 60
Autores: Luís,Ângelo Filipe Santos; Domingues,Fernanda da Conceição; Amaral,Luísa Maria Jota Pereira.
The dried leaves of Ilex paraguariensis, also known as yerba mate or chimarrão, are used to produce infusions consumed by over 1 million people worldwide, being a traditional drink in several South American countries, particularly, Argentina, Brazil, Uruguay, and Paraguay. The positive effects of I. paraguariensis in obesity have been described and observed in some clinical trials both in animals and in humans. The main goal of this work was to perform a systematic review, complying with the PRISMA (Preferred Reported Items for Systematic Reviews and Meta-Analysis) recommendations, followed by a meta-analysis and Trial Sequential Analysis (TSA), to summarize the results of the recent clinical trials, clarifying the anti-obesity potential of I....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis/systematic review; Meta-analysis; Clinical trials; Anti-obesity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100700

Development of self-nanoemulsifying tablet (SNET) for bioavailability enhancement of sertraline
Provedor de dados: 60
Autores: Rahman,Mohammad Akhlaquer; Mujahid,Mohammad.
Abstract The purpose of the study was to combine the advantages of self-nanoemulsifying drug delivery systems and tablets as a conventional dosage form. Self-nanoemulsifying drug delivery system (SNEDDS) was prepared to enhance the solubility and thus oral bioavailability of sertraline. Aqueous titration method was used to prepare the liquid SNEDDS; ternary phase diagrams were constructed and based on smaller droplet size (24.8 nm), minimum viscosity (153.63 cP) and polydispersity index (0.182), higher percentage transmittance (95%) and in vitro drug release (97%), an optimum system was designated. Liquid SNEDDS was transformed into free-flowing powder by solid adsorption technique followed by compression into tablets. In vitro release of sertraline from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SNEDDS; Phase diagrams; Solubility; Thermodynamic stability; Oral delivery; Bioavailability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100620

Determination of in vitro antidiabetic effects of Zingiber officinale Roscoe
Provedor de dados: 60
Autores: Sattar,Naila Abdul; Hussain,Fatma; Iqbal,Tahira; Sheikh,Munir Ahmad.
Aqueous extracts of Zingiber officinale rhizomes were studied to evaluate their antidiabetic effects on protein glycation and on the diffusion of glucose in vitro in the present study. Zingiber officinale rhizome aqueous extract were examined at concentrations of 5, 10, 20 and 40 g/L. The antidiabetic effects were found to be dose-dependent. Antidiabetic potential of Zingiber officinale was mainly through inhibition of the glucose diffusion and to a limited extent by reducing the glycation. However, further studies are needed to determine in vitro effects of therapeutic potential by restraining postprandial glucose absorptions and plasma protein glycations in diabetic subjects.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zingiber officinale/pharmacognosy; Zingiber officinale/antidiabetic effects; Zingiber officinale/in vitro study; Diabetes mellitus; Hyperglycemia; Proteins/glycation; Glucose/diffusion in vitro.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400003

Simple and rapid spectrophotometric assay of albendazole in pharmaceuticals using iodine and picric acid as CT complexing agents
Provedor de dados: 60
Autores: Swamy,Nagaraju; Basavaiah,Kanakapura.
Two simple, rapid and inexpensive spectrophotometric methods are described for the determination of albendazole (ALB) in bulk drug and in tablets. The methods are based on charge-transfer (CT) complexation reaction involving ALB as n-donor and iodine as σ-acceptor (method A) in dichloromethane or picric acid (PA) as π-acceptor (method B) in chloroform. The absorbance of CT complexes was measured at 380 nm for method A, and 415 nm for method B. The optimization of the experimental conditions is described. Under optimum conditions, Beer's law obeyed over the concentration ranges 8.0-240 and 2.4-42 μg mL-1 for method A and method B, respectively. The apparent molar absorptivity of CT complexes at the respective λmax are calculated to be 1.17×103 and 5.22×103...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albendazole/determination; Spectrophotometry/quantitative analysis; Tablets/quantitative analysis; Charge-transfer complexes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400839

Quality indicators of pharmacists' services in community pharmacies in Paraná State, Brazil
Provedor de dados: 60
Autores: Hipólito Júnior,Edson; Halila,Gerusa Clazer; Reis,Walleri Christini Torelli; Guimarães,Matheus Murmel; Guanaes,Laís Danciguer; Pontarolo,Roberto; Correr,Cassyano Januário.
ABSTRACT Pharmacists and their pharmacies have been evolving in their roles as health promoters in Brazil. Some examples are the recent legislation reaffirming the role of Brazilian pharmacies as health institutions, rather than having only a commercial profile, giving greater clarity to pharmacists about their roles as health care providers. This evolution came with the recognition that is already seen in other developed countries, confirming the need for the pharmacist as a health promoter, and not simply a dispenser of drugs in society. This study has obtained the profile and activities of community pharmacists, as well as the quality indicators of private community pharmacies throughout the State of Paraná through the application of an online survey...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care. Pharmacists/professional practice. Community pharmacy/structure indicators/Paraná State; Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100615

Nonadherence to treatment recommendations is a factor contributing to the clinical failure of daptomycin: Cohort study in Brazil
Provedor de dados: 60
Autores: Martins,Tathiana Silva de Souza; Figueras,Albert; Souza,Luana dos Reis de; Santos,Keila Cristina Oliveira dos; Oliveira,Elaine Machado de; Secoli,Silvia Regina.
To evaluate the clinical outcomes of daptomycin therapy and adherence to treatment recommendations, a retrospective cohort study was conducted with patients that received daptomycin during the period of the study. The adherence and nonadherence to clinical guidelines were assessed through organism identification, dose and time of treatment, management of bacteremia, and vancomycin treatment failure. A multiple logistic regression model analyzed the association between independent variables and clinical success (dependent variable), considering 5% of statistical significance. The study presented 52 patients who received daptomycin for the treatment of bacteremia (21.1%) or infections (osteomyelitis [63.5%], synovial fluid [15.4%]). Most patients (86.5%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daptomycin; Post marketing; Gram-positive bacterial infections; Orthopedic.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100583

Pharmacological and toxicological investigations of etodolac loaded gum katira microspheres prepared by W1/O/W2 emulsion solvent evaporation technique in rats
Provedor de dados: 60
Autores: Ruhidas,Biswajit; Ray,Rajat; Naskar,Debjyoti; Chakra,Biplab Kumar; Chatterjee,Tapan Kumar.
ABSTRACT Etodolac is a non-steroidal anti-inflammatory drug (NSAID) and approved by USFDA as a COX2 inhibitor. Although etodolac therapy provides clinical benefits, it is associated with upper gastrointestinal (GI) tract complications also. Etodolac loaded gum Katira microsphere (ELGKM) was prepared by W1/O/W2 emulsion solvent evaporation technique. The gastric irritation properties of orally administered pure etodolac, ELGKM and blank microspheres (without etodolac) were evaluated in experimental rats treated for 6 days. The stomach examination and biochemical investigation of stomach tissue of treated rats indicated that ELGKM formulation remarkably reduced ulcerogenecity as compared to pure etodolac. The anti-inflammatory activities of pure etodolac and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Etodolac/inflammatory conditions; Etodolac/physicochemical characterization; Gum Katira/microspheres; Pain therapy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400619

In vitro skin penetration of clobetasol from lipid nanoparticles: drug extraction and quantitation in different skin layers
Provedor de dados: 60
Autores: Silva,Luís Antônio Dantas; Taveira,Stephânia Fleury; Lima,Eliana Martins; Marreto,Ricardo Neves.
Clobetasol propionate (CP) is a potent topical corticosteroid that causes several cutaneous and systemic side effects. In the present work, CP was encapsulated in nanostructured lipid carriers (NLCs) to increase drug retention in the outer skin layers and improve the safety of topical therapy. NLCs were prepared using a microemulsion technique with a mixture of lecithin, taurodeoxycholate, stearic acid, and oleic acid. In vitro penetration studies were performed in a modified Franz-type diffusion cell, and porcine ears were used as a model of human skin. A simple and sensitive liquid chromatographic method was developed and validated for clobetasol determination in different skin layers. NLCs presented uniform size distribution, high zeta potentialand...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clobetasol propionate/in vitro skin penetration; Nanostructured lipid carriers/in vitro study.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400025

Continuous wavelet transform methods for the simultaneous determinations and dissolution profiles of valsartan and hydrochlorothiazide in tablets
Provedor de dados: 60
Autores: Dinç,Erdal; Üstündağ,Özgür; Tilkan,Günseli Yüksel; Türkmen,Berna; Özdemir,Nurten.
ABSTRACT Continuous wavelet transform (CWT) was proposed for the simultaneous determination and dissolution profiles of valsartan (VAL) and hydrochlorothiazide (HCT) in tablets, without the use of a chemical separation procedure. The CWT approach was applied to the original UV spectra and their ratio spectra in the optimal wavelength ranges. After testing several wavelet families, Mexican hat function-CWT and Daubechies7-CWT (mexh-CWT and db7-CWT, respectively) were found to be suitable for the transformation of the original UV spectra. In the following procedure, mexh-CWT and Coiflets3-CWT (coif3-CWT) were found to be appropriate for the signal analysis of ratio spectra (RS) of VAL/HCT and HCT/VAL. Calibration graphs for VAL and HCT were obtained by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spectral Continuous Wavelets Transform; Valsartan; Hydrochlorothiazide; Binary mixture/tablets.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100618

Toxicological evaluation of Lafoensia pacari A. St.-Hil. (Lythraceae) stem bark extract: Acute and subchronic studies in mice
Provedor de dados: 60
Autores: Ávila,Renato Ivan de; Ferreira,Camila Carvalho; Alvarenga,Cátia Belo Mattos; Vieira,Marcelo de Sousa; Cortez,Alane Pereira; Batista,Aline Carvalho; Costa,Elson Alves; Valadares,Marize Campos.
This study evaluated the safety of Lafoensia pacari A. St.-Hil. (Lythraceae) stem bark hydroalcoholic extract (LPE) through acute and subchronic toxicological assessments in mice. In the acute toxicity evaluation, a single 2000 mg/kg oral dose of LPE was administered to mice and clinical observations were conducted for 14 days. For subchronic toxicity, LPE doses (6.25-1000 mg/kg) were administered orally for 28 days and biochemical, hematological, histopathological analyses and renal and liver expression of Ki-67 were carried out. The acute oral toxicity evaluation of LPE showed no toxicity in mice and it was was classified as category 5 (LD50&gt;2000-5000 mg/kg). In a repeated dose 28-day toxicity study, LPE (100-1000 mg/kg) led to an increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangava-brava; Lafoensia pacari/evaluation; Medicinal plant; Natural product.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100595

Robustness evaluation of the chromatographic method for the quantitation of lumefantrine using Youden's test
Provedor de dados: 60
Autores: César,Isabela da Costa; Pianetti,Gerson Antônio.
Youden's test is a reliable method to evaluate the robustness of analytical methods, by means of an experiment design which involves seven analytical parameters combined in eight tests. In the present study, we assessed the robustness of a chromatographic method to quantify lumefantrine in raw material samples, using Youden's test. Hence, it was possible to determine the effect of each analytical parameter in the final analysis results. Youden's test showed to be a simple and feasible procedure to evaluate the robustness of chromatographic methods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lumefantrine/determination; Youden's test; High Performance Liquid Chromatography/quantitative analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200007

Pharmaceutical services and health promotion: how far have we gone and how are we faring? Scientific output in pharmaceutical studies
Provedor de dados: 60
Autores: Nakamura,Carina Akemi; Soares,Luciano; Farias,Mareni Rocha; Leite,Silvana Nair.
The objective of this study was to investigate the scientific output on health promotion within the pharmaceutical field and its relation with the development of pharmaceutical services within health systems. A comprehensive review of published scientific articles from the Medline and Lilacs databases was carried out. The review comprised articles published until December 2011, and used combinations of the terms 'health promotion' or 'health education' and 'pharmacy', 'pharmacist' or 'pharmaceutical'. The articles were selected according to inclusion and exclusion criteria. A total of 170 full texts and 87 indexed abstracts were analyzed, evidencing that most described actions of health promotion in community pharmacies and other services. Following the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Health promotion; Health education; Pharmaceutical care/scientific production; Pharmacists/professional practice; Pharmacy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400773

Development and validation of a rapid turbidimetric assay to determine the potency of norfloxacin in tablets
Provedor de dados: 60
Autores: Chierentin,Lucas; Salgado,Hérida Regina Nunes.
Norfloxacin is one of the first commercially available (and most widely used) fluoroquinolone antibiotics. This paper reports the development and validation of a simple, sensitive, accurate and reproducible turbidimetric assay method to quantify norfloxacin in tablets formulations in only 4 hours. The bioassay is based on the inhibitory effect of norfloxacin upon the strain ofStaphylococcus epidermidis ATCC 12228 IAL 2150 used as test microorganism. The assay was performed 3x3 parallel lines like, three tubes for each concentration of reference substance and three tubes for each sample concentration. The results were treated statistically by analysis of variance and were found to be linear (r2 = 0.9999) in the selected range of 25-100 μg mL-1; precise...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiological assay; Quality control; Norfloxacin/quantification in tablets; Turbidimetric method/quality control/validation; Antimicrobials/quality control.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300629

Volatile compounds and palynological analysis from pollen pots of stingless bees from the mid-north region of Brazil
Provedor de dados: 60
Autores: Lima Neto,José de Sousa; Lopes,José Arimatéia Dantas; Moita Neto,José Machado; Lima,Sidney Gonçalo de; Luz,Cynthia Fernandes Pinto da; Citó,Antônia Maria das Graças Lopes.
ABSTRACT Samburá is the botanical pollen nectar agglutinated by salivary secretions of bees. Stingless bee pollen samples were collected in three periods of the year in Monsenhor Gil town, PI, Brazil, for extraction of volatile constituents by different techniques, analyzed by gas chromatography-mass spectrometry (GC-MS) and the palynological analysis used to identify the dominant pollen. Among the volatile compounds identified, kaur-16-ene, methyl and ethyl hexadecanoate, methyl linoleate and heneicosane were identified more frequently in the studied parameters: period of sample collection and extraction techniques used. The palynological analysis identified the pollen of Mimosa caesalpiniifolia Benth. as the dominant pollen in all samples studied.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pollen/Scaptotrigona sp; Mimosa caesalpiniifolia Benth; Pollen/volatile constituents; Chromatography-mass spectrometry; Palinology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200619

Enzyme inhibition and antibacterial potential of 4-Hydroxycoumarin derivatives
Provedor de dados: 60
Autores: Saleem,Afia; Bukhari,Syed Majid; Zaidi,Asma; Farooq,Umar; Ali,Majid; Khan,Asma; Khan,Sidra; Shah,Kausar Hussain; Mahmood,Adeem; Khan,Farhan Ahmed.
The 4-Hydroxycoumarin derivatives are known to show a broad spectrum of pharmacological applications. In this paper we are reporting the synthesis of a new series of 4-Hydroxycoumarin derivatives synthesized through Knovenegal condensation; they were characterized by using UV-Vis, FT-IR, NMR spectroscopies. The synthesized compounds were evaluated for antibacterial activity against Staphylococcus aureus and Salmonella typhimurium strains. The compounds (2), (3) and (8) showed favorable antibacterial activity with zone of inhibitions 26.5± 0.84, 26.0 ± 0.56 and 26.0 ± 0.26 against Staphylococcus aureus (Gram-positive) respectively. However, the compounds (5) and (9) were found more active with 19.5 ± 0.59 and 19.5 ± 0.32 zone of inhibitions against...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-Hydroxycoumarin; Anti-urease; Carbonic anhydrase; Aldehydes; Antibacterial.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100580

Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules
Provedor de dados: 60
Autores: Ianiski,Francine Rodrigues; Laporta,Luciane Varini; Rubim,Alexandre Machado; Luchese,Cristiane.
abstract A method to ensure that an analytical method will produce reliable and interpretable information about the sample must first be validated, making sure that the results can be trusted and traced. In this study, we propose to validate an analytical high performance liquid chromatography (HPLC) method for the quantitation of meloxicam loaded PEGylated nanocapsules(M-PEGNC). We performed a validation study, evaluated parameters including specificity, linearity, quantification limit, detection limit, accuracy, precision and robustness. PEGylated nanocapsules were prepared by interfacial deposition of preformed polymer, and the particle size, polydispersity index, zeta potential, pH value and encapsulation efficiency were characterized. The proposed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography/quantitative analysis; Meloxicam/determination; PEGylated nanocapsules/quality control; Nanoparticles; Poly(ethylene glycol).
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400823

A smart approach for delivery of aripiprazole via oro-soft palatal mucosal route for improved therapeutic efficacy
Provedor de dados: 60
Autores: Madhav,Nookala Venkala Satheesh; Ojha,Abhijeet; Jaiswal,Vishakha.
Effective management of schizophrenia, acute mania, mixed episodes associated with bipolar disorders, and depression can be managed with aripiprazole moiety. In the present research work an attempt was made to minimize the dose related side effects thus improving the quality life of the patients. A novel biopolymer was isolated from the fruits of Trachyspermum ammi. Ten optimized nanosized aripiprazole loaded formulations were prepared in 1-5% concentration of biopolymer (FA1-FA5) and sodium CMC (FM1-FM5) by solvent casting technique. The formulated flexy films were evaluated for thickness, folding endurance, weight uniformity, surface pH, mucoadhesivity, In-vitro drug release studies, In-vivo pharmacodynamic study and stability studies. The isolated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aripiprazole/therapeutic efficacy; Nanosized; Mucoadhesive; Schizophrenia; Oro-soft palatal mucosal route.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300611

The period between beta-blocker use and physical activity changes training heart rate behavior
Provedor de dados: 60
Autores: Alves,Naiane Ferraz Bandeira; Porpino,Suênia Karla Pacheco; Silva,Alexandre Sérgio.
The Brazilian Society of Cardiology (SBC) proposes that hypertensive subjects who use beta-blockers and practice physical exercises must have their training heart rate (HR) corrected due to the negative chronotropic effect of this drug. Nevertheless, if the physical activity is performed outside of plasmatic half-life, correction may not be necessary. This study investigated the exercise chronotropic response both inside and outside the beta-blocker plasmatic half-life. Nine subjects in use of atenolol or propranolol, and six controls, carried out three walking sessions in three days according to different schedules: EX2 (two hours after drug administration, at the plasmatic peak); EX11 (eleven hours after drug administration, at the end of plasmatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta-blockers/negative chronotropic effect; Atenolol; Propanolol; Hypertension; Physical exercise; Training heart rate/control.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400017

Study on the development of nanotechnology in advanced countries and in Brazil
Provedor de dados: 60
Autores: Rediguieri,Carolina Fracalossi.
The study shows how nanotechnology evolves in developed countries and Brazil, raising aspects of private and governmental initiatives. The investigation was based in scientific literature, electronic articles and conference reports. Several sources of literature were used, including electronic databases and reference lists. By this study, it was observed that, although nanotechnology is in initial stage of development all over the world, the developed countries have had growing public and private investments in the area each year. In those countries, there is a concern toward both, the formation of specialists in nanotechnology and the transference of technology developed in universities and research institutes to industry. In Brazil, the study showed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanotechnology/development; Nanobiotechnology; Nanopharmaceutical.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200002

Unsymmetrically substituted imidazolium salts: synthesis, characterization and antimicrobial activity
Provedor de dados: 60
Autores: Çoban,Esin Poyrazoğlu; Fırıncı,Rukiye; Biyik,Halil; Günay,Muhammet Emin.
ABSTRACT Unsymmetrically substituted imidazolium salts were synthesized and characterized using 1H-NMR and 13C-NMR. The antimicrobial activities of the salts were evaluated using the agar-well diffusion method against 14 bacteria and five yeasts. The minimal inhibitory concentrations (MIC) against seven bacteria and one yeast were also determined. Among the test compounds applied, 1, 2, 3, 6 and 11 showed activities against Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Bacilllus cereus ATCC 11778, Bacillus subtilis ATCC 6633, Bacillus thuringiensis, Listeria monocytogenes ATCC 19112 and Candida trophicalis. However, compounds 1, 2 and 3 showed the highest antimicrobial activities against Micrococcus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Imidazolium salts/characterization; N-heterocyclic carbenes; Imidazolium salts/antimicrobial activity; Imidazolium salts/Minimum inhibitory concentration.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100604

Test of dissolution and comparison of in vitro dissolution profiles of coated ranitidine tablets marketed in Bahia, Brazil
Provedor de dados: 60
Autores: Santos Júnior,Aníbal de Freitas; Barbosa,Igor Santos; Santos,Venyson Lima dos; Silva,Rangel Leal; Caetite Junior,Edimar.
Ranitidine is an antisecretory drug with H2 antagonist action useful in treating gastric and duodenal disorders. The dissolution test is used to obtain and compare dissolution profiles and establish similarities of pharmaceutical forms. The aim of this study was to compare the dissolution profiles of 150-mg coated ranitidine tablets of a reference drug (product A) and a generic (product B) and a similar (product C) drug marketed in Bahia, Brazil using a simple, fast and inexpensive ultraviolet method. Dissolution was determined using a USP type 2 apparatus at 50 rpm with 900 mL of distilled water at 37.0 ± 0.5 oC for 1h. The dissolution test was performed in compliance with the American Pharmacopoeia (USP-32). Dissolution efficiency and difference (f1) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ranitidine/coated tablets/dissolution test; Coated tablets/dissolution test; Spectrophotometry/drug analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100008

Is the efflux pump inhibitor Verapamil a potential booster for isoniazid against Mycobacterium tuberculosis?
Provedor de dados: 60
Autores: Amaral,Renata Claro Ribeiro do; Caleffi-Ferracioli,Katiany Rizzieri; Demitto,Fernanda de Oliveira; Almeida,Aryadne Larissa de; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin de Lima; Leite,Clarice Queico Fujimura; Pavan,Fernando Rogério; Cardoso,Rosilene Fressatti.
The membrane-based efflux pump systems are recognized to have an important role in pathogenicity and drug resistance in Mycobacterium tuberculosis by the extrusion of toxic substrates and drugs from the inner bacillus. This study aimed to investigate the in vitro interaction of Verapamil (VP), an efflux pump inhibitor, with the classical first-line anti-tuberculosis drug isoniazid (INH) in resistant and susceptible M. tuberculosis clinical isolates. Seven multidrug-resistant (MDR), three INH monoresistant and four susceptible M. tuberculosis clinical isolates were tested for the INH and VP combination by modified Resazurin Microtiter Assay Plate (REMA). Fractional Inhibitory Concentration (FIC) and Modulation Factor (MF) were determined. The INH plus VP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Multidrug-resistance; Efflux pumps; Efflux pumps inhibitors; Isoniazid.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100588

In vitro hypoglycemic effects of unripe and ripe fruits of Musa sapientum
Provedor de dados: 60
Autores: Bhinge,Somnath Devidas; Bhutkar,Mangesh Anil; Randive,Dheeraj Suhas; Wadkar,Ganesh Hindurao; Hasabe,Tejashri Suresh.
ABSTRACT The present study was undertaken to verify the hypoglycemic potential of unripe and ripe fruit extracts of Musa sapientum by using various in-vitro techniques, namely glucose adsorption capacity, glucose diffusion, amylolysis kinetics and glucose transport across the yeast cells. The results revealed that the unripe and ripe fruit extracts of Musa sapientum adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. There were no significant (p≤0.05) differences between their adsorption capacities. In the amylolysis kinetic experimental model the rate of glucose diffusion was found to be increased with time from 30 to 180 min and both extracts exhibited significant inhibitory effects on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Hypoglycemic effect/study; Glucose diffusion; Yeast cells; Musa sapientum/effects.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400605

Extract from leaf of Psidium guajava L depresses the guinea pig atrial contractility by interfering with potassium and calcium channels
Provedor de dados: 60
Autores: Gondim,Antonio Nei Santana; Oliveira,Vanda Rodrigues de; Santos,Sellyanna Domeny dos; Silva,Bagnólia Araújo da; Vasconcelos,Carla Maria Lins de; Conde-Garcia,Eduardo Antonio.
The negative inotropic effect of aqueous fraction (AqF) obtained from the acetic extract of Psidium guajava L leaf was investigated on the guinea pig left atrium. Myocardial force was measured isometrically (27 ± 0.1 ºC, 2 Hz). AqF (100 μg/ml) reduced contractility of about 85 ± 9.4 % (n = 4, p &lt; 0.001, Fcalc = 51.70, F(0.01; 4; 21) = 5.09, EC50 = 14.28 ± 3 μg/mL) in a concentration-dependent fashion. This effect was reduced by 20 mM of tetraethylammonium (TEA), increasing EC50 to 50 ± 7 μg/ml (n = 4, p &lt; 0.001, Fcalc = 282.13; F(0.01; 21; 66) = 2.36). AqF (100 μg/ml) shifted to the right the CaCl2 concentration-effect curve, increasing the EC50 from 2170 ± 112 to 2690 ± 132 μM (n = 3, p &lt; 0.001, Fcalc = 220.80 ; F(0.01; 29; 60) = 2.19). L-NAME...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psidium guajava/acetic extract; Psidium guajava/inotropic effect; Inotropic effect/experimental study; Myocardium/contraction; Myocardium/experimental studies; Myocardial cells; Experimental studies.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300014

Quantification and stress degradation studies of cefepime/tazobactam in dry injection form by an RP-HPLC method
Provedor de dados: 60
Autores: Kommana,Ramakrishna; Kannabattula,Gouthami; Gurrala,Sunitha; Yeradesi,Venkat Raj; Durga,Panikumar Anumolu.
A simple, specific, precise, accurate, linear, rapid, economic and validated stability indicating an RP-HPLC method for the simultaneous quantification of cefepime and tazobactam in a dry injection dosage form has been developed. Separation was performed on a 5 µm ACE C18column with phosphate buffer, pH adjusted to 4.5 with phosphoric acid: methanol (70:30) at a flow rate of 1 mL/min and at a temperature of 25 °C. Regression analysis showed linearity at a detector wavelength of 290 nm in the range of 200-600 μg/mL for cefepime and 25-75 μg/mL for tazobactam. All of the analytes were adequately resolved with acceptable tailing. The percentage content found for cefepime was 99.98% and of tazobactam was 99.49% in the parenteral formulation. The method was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography/quantitative analysis; Cefepime/quantificação; Tazobactam/quantificação; Dry injection formulation/quantitative analysis; Pharmaceutical formulations/degradation studies.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400895

Anti-diabetic effects of Campomanesia xanthocarpa (Berg) leaf decoction
Provedor de dados: 60
Autores: Vinagre,Anapaula Sommer; Rönnau,Ângela Della'Santa Rubio Origuella; Pereira,Sabrina Francisco; Silveira,Lídia Uliano da; Wiilland,Elenir de Fátima; Suyenaga,Edna Sayuri.
The objective of this research was to identify the effects of 3-week treatment of normal and streptozotocin-induced diabetic rats using a leaf decoction of Campomanesia xanthocarpa Berg. (20 g/L) on physiological, biochemical and histological parameters. Streptozotocin (STZ, 70 mg/kg in citrate buffer, pH 4.5) was administered IP to induce experimental diabetes one week prior to the treatment. STZ caused typical diabetic symptoms: polydypsia, polyuria, polyphagia, hyperglycemia, hypertriglyceridemia and histopathological modifications in the pancreas, liver and kidney. The treatment of diabetic rats using the decoction decreased blood glucose levels, inhibited hepatic glycogen loss, and prevented potential histopathological alterations in the pancreas and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campomanesia xanthocarpa/pharmacognosy; Diabetes Mellitus/experimental study; Hypoglycemic plant; Histology; Carbohydrate/metabolism; Lipid/metabolism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200002

Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds
Provedor de dados: 60
Autores: Nawaz,Haq; Shad,Muhammad Aslam; Rehman,Najiha; Andaleeb,Hina; Ullah,Najeeb.
The effect of solvent polarity on extraction yield and antioxidant properties of phytochemical compounds in bean seeds was studied. Seed flour of three varieties of bean was extracted in a series of organic solvents with increasing polarity (n-hexane, petroleum ether, chloroform, ethyl acetate, ethanol, acetone and water). Preliminary screening of phytochemicals showed the presence of tannins, flavonoids, cardiac glycosides, anthocyanins, terpenoids, carotenoids, ascorbic acid and reducing compounds in all extracts. One way analysis of variance (ANOVA) of results showed that extraction yield, phytochemical content and antioxidant properties were significantly influenced (p&lt;0.05) by the polarity of extracting solvents. The regression analysis of data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beans seeds/antioxidant properties; Phaseolus vulgaris/phytochemical composition; Regression analysis; Solvent polarity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100501

Quercetin modulates age-induced changes in the transcript levels of some apoptosis related genes in the skeletal muscles of male rats
Provedor de dados: 60
Autores: Alm-Eldeen,Abeer; Khamis,Abeer; Elfiky,Nabil; Ahmad,Randa.
Adverse changes occur gradually in the skeletal muscles with age via continuous exposure to oxidative stress. Quercetin, a member of the flavonoids family, possesses anti-oxidative and radical-scavenging activities. Therefore, this study investigated the role of quercetin to modulate age-induced changes in the transcript levels of some apoptosis-related genes in rat’s gastrocnemius muscles, up to 15 months-old. Half of the rats at each age (1, 5, 10 and 15 months old) were given a vehicle and the other half was given 200 mg/kg quercetin for 2 weeks, respectively. With the increase of age, vehicle groups showed hyalinization of the muscle fibers and a decrease of the catalase and an increase of the malondialdehyde levels. Down-regulation of Bcl2 gene and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quercetin Age; Bcl2 Bax NF-KB Apoptosis Caspace3 Catalase MDA Histopathology.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100638

Analysis of advertisements of infant food commercialized in the city of Natal, Rio Grande do Norte, Brazil
Provedor de dados: 60
Autores: Pagnoncelli,Maria Giovana Binder; Batista,Almária Mariz; Silva,Michelle Cristine Medeiros Da; Costa,Ana Paula Marques Da; Araújo,Fábio Resende De; Marques,Marcela Pinheiro; Fidalgo,Cláudia M. De Quelhas; Carvalho,Maria Cleide Ribeiro Dantas de.
The advertising about maternal milk substitutes has been pointed as one of the factors responsible for the breastfeeding low rates. In this way, legal support was created to assure that the use of such products would not interfere on the healthy breastfeeding habit of the population. This study, developed between June 2006 and May 2008 in the city of Natal, state of Rio Grande do Norte, analyzed the food advertisements divulged under the validity of Law # 11.265/2006, which controls in Brazil the food commercialization and the publicity addressed to parents or keepers of nursling and children in the first childhood. 220 advertisements, being 141 of technical-scientific nature, and 79 for strictly commercial promotion, were collected and evaluated according...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast-feeding; Food advertising/analysis; Maternal milk/substitutes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200020

Twenty years of LASSBio, the laboratory of evaluation and synthesis of bioactive substances!
Provedor de dados: 60
Autores: Ferreira,Elizabeth Igne.
Tipo: Info:eu-repo/semantics/other
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300005

Drug advertising directed to pharmacists in Brazil: information or sales promotion?
Provedor de dados: 60
Autores: Soares,Jussara Calmon Reis de Souza.
Analyses of drug advertising reveal important social and cultural values and attitudes at a certain point in history. The purpose of this paper was to investigate how pharmaceutical industry communicates with pharmacists in Brazil, using drug promotion as a valuable tool. The point of departure was the analysis of a series of drug advertisements published in three Brazilian technical journals targeted at pharmacists and other health professionals. For the present study, the focus was on the content of the messages directed to pharmacists, in order to critically analyze the role attributed to these professionals as portrayed by the ads, and to discuss it in the context of pharmaceutical care. The collection and analysis of the data followed Anvisa's...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/advertising; Pharmacists; Pharamceutical care.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400004

Toxicity assessment of the alcoholic leaves extract of Reinwardtia indica
Provedor de dados: 60
Autores: Upadhyay,Prabhat; Shukla,Rashmi; Tiwari,Kavindra Nath; Dubey,G P; Mishra,Sunil Kumar.
The present study aimed to evaluate the safety of the alcoholic leaves extract of Reinwardtia indica in Charles foster rats through an acute and sub-acute oral administration.For assessment of acute oral toxicity test, ratswere orally treated with single dose of the alcoholic leaves extract of Reinwardtia indica at the doses of 50, 250, 500, 1000 2000 and 5000 mg/kg. In sub-acute toxicity study, using the OECD guidelines no. 407, the extract was administered at the doses of 50, 250, 500, 1000, 2000 mg/kg/day for 28 consecutive days and at the dose of 2000 mg/kg satellite group also used for 6 weeks.In acute toxicity above mentioned doses neither showed mortality nor exterior signs of toxicity. In sub-acute, study no significant changes found in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reinwardtia indica; Biochemical test; Hematological test; Histopathology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100511

Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil
Provedor de dados: 60
Autores: Oltramari,Karine; Cardoso,Rosilene Fressati; Patussi,Eliana Valéria; Santos,Adolfo Carlos Barreto; Mikcha,Jane Martha Graton.
Food contamination caused by enteric pathogens is a major cause of diarrheal disease worldwide, resulting in high morbidity and mortality and significant economic losses. Bacteria are important agents of foodborne diseases, particularly diarrheagenic Escherichia coli. The present study assessed the genetic diversity and antimicrobial resistance of E. coli isolates from pasteurized milk processed in 21 dairies in northwestern State of Parana, Brazil. The 95 E. coli isolates were subjected to antimicrobial susceptibility testing according to the recommendations of the Clinical and Laboratory Standards Institute and assessed genotypically by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The highest rate of resistance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pasteurized milk/processing; Pasteurized milk/bacterial typing; Pasteurized milk/contamination; Escherichia coli/genetic diversity; Escherichia coli/antimicrobial resistance; Food/microbiological analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200337

Determination of olanzapine by spectrophotometry using permanganate
Provedor de dados: 60
Autores: Rajendraprasad,Nagaraju; Basavaiah,Kanakapura.
Two new spectrophotometric methods using permanganate as the oxidimetric reagent for the determination of olanzapine (OLP) were developed and validated as per the current ICH guidelines. The methods involved the addition of known excess of permanganate to OLP in either acid or alkaline medium followed by the determination of unreacted permanganate at 550 nm (method A) or bluish-green color of manganate at 610 nm (method B). The decrease in absorbance in method A or increase in absorbance in method B as a function of concentration of OLP was measured and related to OLP concentration. Under optimized conditions, Beer's law was obeyed over the ranges 2.0 to 20 and 1.0 to 10 μg mL-1 in method A and method B, respectively. The calculated molar absorptivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olanzapine/determination; Spectrophotometry; Permanganate; Pharmaceuticals.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300020

Exendin-4 effects on islet volume and number in mouse pancreas
Provedor de dados: 60
Autores: Khorsandi,Layasadat; Nejad-Dehbashi,Fereshteh.
The aim of this study was to evaluate Exendin-4 (EX-4) effects on islet volume and number in the mouse pancreas. Thirty-two healthy adult male NMRI mice were randomly divided into control and experimental groups. EX-4 was injected intraperitoneally (i. p.) at doses of 0.25 (E1 group), 0.5 (E2 group), and 1 µg/kg (E3 group), twice a day for 7 consecutive days. One day after the final injection, the mice were sacrificed, and the pancreas from each animal dissected out, weighed, and fixed in 10% formalin for measurement of pancreas and islet volume, and determination of islet number by stereological assessments. There was a significant increase in the weight of pancreases in the E3 group. Islet and pancreas volumes in E1 and E2 groups were unchanged compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exendin-4/effects; Exendin-4/experimental study; Pancreas/effects fo Exendin-4; Islet/hypertrophy; Stereology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400014

Evaluation of medicine package inserts: a study of two cases of Pelargonium sidoides D.C. phytomedicines
Provedor de dados: 60
Autores: Linhares,Tiago Duarte; Oliveira,Danilo Ribeiro de; Silva,Michele Feitoza.
In order for a phytotherapeutic drug be approved and sold in Brazil, it must be registered with the National Sanitary Surveillance Agency (ANVISA), where the quality, safety and efficacy of the product are carefully appraised. In addition, the drug must also meet a number of criteria one of which is the adequacy of the package inserts. Therefore, the aim of this study was to appraise the package inserts of all phytotherapeutic drugs produced using a standardized extract of Pelargonium sidoides, which were registered and available from Brazilian pharmacies. This checking was to ascertain whether these inserts fulfilled the requirements stipulated by RDC 140/03. The information required under RDC 140/03 was appraised through a previously devised standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/formulation of package inserts; Phytotherapeutic drugs; Package inserts/evaluation; Pelargonium sidoides.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100008

Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR
Provedor de dados: 60
Autores: Caleffi-Ferracioli,Katiany Rizzieri; Baldin,Vanessa Pietrowski; Noguti,Érika Noda; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin Lima; Pavan,Fernando Rogério; Hirata,Mario Hiroyuki; Cardoso,Rosilene Fressatti.
Tuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Molecular characterization; ERIC-PCR; Epidemiology.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300616

Solid dispersion-based pellet for colon delivery of tacrolimus through time- and pH-dependent layer coating: preparation, in vitro and in vivo studies
Provedor de dados: 60
Autores: Guo,Jian; Fang,Huihui; Gui,Shuangying; Huang,Yuzhe.
The intent of the present investigation is to develop and evaluate colon-specific coated tacrolimus solid dispersion pellet (SDP) that retards drug release in the stomach and small intestine but progressively releases in the colon. Tacrolimus-SDP was prepared by extrusion-spheronization technology and optimized by the micromeritic properties including flowability, friability, yields and dissolution rate. Subsequently, the pH-dependent layer (Eudragit L30D55) and time-dependent layer (Eudragit NE30D and L30D55) were coated on the SDP to form tacrolimus colon-specific pellets (CSP) using a fluidized bed coater. Under in vitro gradient pH environment, tacrolimus only released from CSP after changing pH to 6.8 and then quickly released in the phosphate buffer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tacrolimus; Solid dispersion; Pellet; Oral colon-specific; Biodistribution.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100557

Development of enteric coated tablets from spray dried extract of feverfew (Tanacetum parthenium L: )
Provedor de dados: 60
Autores: Chaves,Juliana Siqueira; Da Costa,Fernando Batista; Freitas,Luís Alexandre Pedro de.
Tanacetum parthenium (feverfew) is an herb that is commercialized worldwide as a therapeutic treatment for migraine. Its pharmacological effect is mainly due to the presence of the sesquiterpene lactone parthenolide as well as of flavonoids. So far, there are no studies on standardization of pre-formulations or phytomedicines containing this herb. The present study aimed at developing a pre-formulation using a standardized spray-dried extract of feverfew and further designing and standardizing enteric coated tablets. In this work, the spray-dried extract of feverfew was evaluated for its parthenolide, santin and total flavonoid content, parthenolide solubility, particle size, tapped density, hygroscopicity, angle of repose and moisture content. Tablets...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteric coated tablets; Flavonoids; Parthenolide; Phytomedicine; Spray-dried extract; Tanacetum parthenium.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300024

Finasteride promotes worsening of the cardiac deleterious effects of nandrolone decanoate and protects against genotoxic and cytotoxic damage
Provedor de dados: 60
Autores: Mata,Elizângela Faustino Da; Nascimento,Andrews Marques do; Lima,Ewelyne Miranda de; Kalil,Ieda Carneiro; Endringer,Denise Coutinho; Lenz,Dominik; Bissoli,Nazaré Souza; Brasil,Girlandia Alexandre; Andrade,Tadeu Uggere de.
Metabolism of anabolic androgenic steroids is important for its physiological effects. The aim was to investigate the effects of finasteride (a 5α-reductase inhibitor - 5αR) on cardiac and mutagenic effects promoted by ND. Male Wistar rats were separated into three groups: CONT, received the vehicles of ND and finasteride (Peanut oil+Saline); DECA group, received ND (20 mg.kg.week-1, i.m.), and DECAF received ND and finasteride (100 µg.kg-1, i.p.), for four weeks. After, hypertrophy, cytokines and Angiotensin Converting Enzyme (ACE) activity was determined in heart. Bone marrow was used for micronucleus evaluation. Treatment with ND promotes increase in cardiac hypertrophy, ACE activity and disbalance among pro- and anti-inflammatory cytokines, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dehydronandrolone; Finasteride; Mutagenicity; Cardiac Hypertrophy; Anabolic Androgenic Steroids.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100532

The effects of acute administration of the hydroalcoholic extract of rosemary (Rosmarinus officinalis L.) (Lamiaceae) in animal models of memory
Provedor de dados: 60
Autores: Zanella,Camila Angela; Treichel,Helen; Cansian,Rogério Luis; Roman,Silvane Souza.
Rosmarinus officinalis (Rosemary) demonstrates antioxidant, antidepressant, diuretic, antinociceptive and antiulcerogenic activities. The present study was designed to examine the effects of the hydroalcoholic extract of R. officinalis on the memory of male mice. The behavioral tasks employed were social recognition (SR), the Morris water maze (MWM) and an inhibitory avoidance task (IA). The treatment with 150 and 300 mg/kg of R. officinalis improved the acquisition phase of learning of a new social memory in the SR task because a decrease was observed in the duration of social investigation. In the Morris water maze, no significant effect was observed on spatial memory when the groups were compared for the time spent in the correct quadrant. In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosmarinus officinalis/pharmacognosy; Rosmarinus officinalis/hydroalcoholic extract; Rosmarinus officinalis/experimental study; Short-term memory/experimental study; Long-term memory/experimental study; Spatial memory/experimental study.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300005

Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
Provedor de dados: 60
Autores: Utescher,Carla Lilian de Agostini; Buosi,Klariane Laís; Botosso,Viviane Fungaro; Quintilio,Wagner.
ABSTRACT The use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are suitable. Kit transport and storage conditions were also evaluated, proving that an adequate cold chain must be assured to achieve good results. Furthermore, the commercial MAT kit seemed suitable to replace the rabbit pyrogen test (RPT) for pyrogen testing of hyperimmune sera, although further tests are needed to a full validation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocytes Activation Test; Pyrogen; Hyperimmune serum; Pyrodetect kit; Cryoblood.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200605

Leite para adultos: mitos e fatos frente à ciência
Provedor de dados: 60
Autores: Tirapegui,Julio.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400027

Characterization and optimization of oil microcapsules from Attalea phalerata Mart. for the preservation of bioactive compounds
Provedor de dados: 60
Autores: Lima,Fernando Freitas de; Lescano,Caroline Honaiser; Oliveira,Elaine Florinda Rodrigues de; Fakhouri,Farayde Matta; Moraes,Izabel Cristina Freitas; Kassuya,Cândida Aparecida Leite; Sanjinez-Argandoña,Eliana Janet.
This study aimed microencapsulating Attalea phalerata Mart. oil, containing high carotenoid and phenolic compounds content, with Arabic gum and gelatin, using the complex coacervation method. The yield, efficiency, morphology of microcapsules and content of phenolic compounds, carotenoids and antioxidant activity in different processes conditions (concentration of the filling, temperature and agitation speed) were evaluated. The results showed 88% of yield, efficiency up to 70% and a characteristic size of microcapsules. The amount of carotenoids was high in crude oil (394.84 µg of carotenoids/g oil) and the microencapsulation tests showed amounts of 19.19 to 166.40 µg of carotenoids/g oil. The phenolic compounds in the crude oil were 20.73 mg GAE/g sample...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carotenoids; Phenolic compounds; Antioxidant activity; Bioactive compounds; Bioactive maintenance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100580

Alginate-pomegranate peels' polyphenols beads: effects of formulation parameters on loading efficiency
Provedor de dados: 60
Autores: Zam,Wissam; Bashour,Ghada; Abdelwahed,Wassim; Khayata,Warid.
Calcium alginate beads containing pomegranate peels' polyphenol extract were encapsulated by ionic gelation method. The effects of various formulation factors (sodium alginate concentration, calcium chloride concentration, calcium chloride exposure time, gelling bath time maintaining, and extract concentration) on the efficiency of extract loading were investigated. The formulation containing an extract of 1 g pomegranate peels in 100 mL distilled water encapsulated with 3 % of sodium alginate cured in 0.05 M calcium chloride for 20 minutes and kept in a gelling bath for 15 minutes was chosen as the best formula regarding the loading efficiency. These optimized conditions allowed the encapsulation of 43.90% of total extracted polyphenols and 46.34 % of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pomegranate/polyphenols/microencapsulation; Calcium alginate beads/microencapsulation; Microencapsulation; Polyphenols; Natural antioxidants/microencapsulation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400741

Influence of glucose and stirring in the fermentation process in order to produce anti- Candida metabolites produced by  Streptomyces sp.
Provedor de dados: 60
Autores: Escher,Silvia Katrine Silva; Sousa Júnior,José Jeosafá Vieira de; Dias,Adrielle Leal; Amorim,Elba Lúcia Cavalcanti de; Araújo,Janete Magalí de.
ABSTRACT This study evaluated the influence of glucose and stirring in the fermentation process in order to produce anti-Candida metabolites produced by Streptomyces sp. MPO4 isolated from Amazon soil. The anti-Candida metabolites production was registered after 24 h of fermentation in stirred ISP2 medium, having antifungal inhibition halos between 12.3 mm and 25.3 mm, yielding higher production of anti-Candida agents after 96 h. Stirring was a determining factor for the production of anti-Candida secondary metabolites, since the absence of glucose reflected in the late production of the antifungal starting from Streptomyces sp.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptomyces sp./anti-Candida activity; Anti-Candida metabolites/production; Fermentation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200265

Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa
Provedor de dados: 60
Autores: Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Císarová,Miroslava; Javoreková,Soňa.
In the present study we reported the antimicrobial activity of actinomycetes isolated from aridic soil sample collected in Karoo, South Africa. Eighty-six actinomycete strains were isolated and purified, out of them thirty-four morphologically different strains were tested for antimicrobial activity. Among 35 isolates, 10 (28.57%) showed both antibacterial and antifungal activity. The ethyl acetate extract of strain KRG-1 showed the strongest antimicrobial activity and therefore was selected for further investigation. The almost complete nucleotide sequence of the 16S rRNA gene as well as distinctive matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry (MALDI-TOF/MS) profile of whole-cell proteins acquired for strain KRG-1 led to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinomycin/antimicrobial activity; Streptomyces; Soil; 16S rRNA; MALDI-TOF MS.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100594

Antileishmanial in vitro activity of essential oil from Myrciaria plinioides, a native species from Southern Brazil
Provedor de dados: 60
Autores: Kauffmann,Carla; Giacomin,Ana Caroline; Arossi,Kelen; Pacheco,Leandra Andressa; Hoehne,Lucélia; Freitas,Elisete Maria de; Machado,Gérzia Maria de Carvalho; Cavalheiro,Marilene Marcuzzo do Canto; Gnoatto,Simone Cristina Baggio; Ethur,Eduardo Miranda.
In South American folk medicine members of the genus Myrciaria are used for the treatment of malaria, diarrhoea, asthma, inflammation and post-partum uterine cleansing. The aim of this work was to evaluate its antileishmanial properties (in vitro) of essential oil derived from leaves of Myrciaria plinioides D. Legrand, a plant species that is native in South of Brazil. The essential oil was obtained by hydro-distillation using fresh leaves of M. plinioides. The chemical composition of this essential oil (MPEO, M. plinioides essential oil) was determined by gas chromatography coupled to mass spectrometry (GC-MS). MPEO was assayed in vitro for antileishmanial properties against promastigotes of Leishmania amazonensis and Leishmania infantum, and for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antileishmanial activity; Myrciaria plinioides; Myrtaceae; Leishmania amazonensis; Leishmania infantum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100558

Quality of life perception of type 1 diabetic patients treated with insulin analogs and receiving medication review with follow-up in a public health care service from Ponta Grossa-PR, Brazil
Provedor de dados: 60
Autores: Garabeli,Aline Ansbach; Daher,Josélia Borba; Wiens,Astrid; Lenzi,Luana; Pontarolo,Roberto.
ABSTRACT Glycemic control in patients with diabetes mellitus type 1 (DM1) reduces the risk of complications but requires a rigorous health care routine. Thus, diabetes education is central to increasing treatment compliance and self-care practices. This study aimed to evaluate the quality of life (QoL) and glycemic control of DM1 patients being treated with insulin analogs and receiving medication review with follow-up. This was a transversal study that included 110 patients registered at the 3rd Health Regional of Ponta Grossa-PR, aged ≥ 18 years, and receiving pharmaceutical care for at least 1 year. The Diabetes Quality of Life Measure (DQOL)-Brazil was used to evaluate QoL. The data were statistically analyzed using SPSS version 17.0 with 95%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus type 1/treatment; Diabetes mellitus type 1/treatment/quality of life; Pharmaceutical care; Insulin analogs; Pharmacotherapy follow-up.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400669

Stability and in vivo efficiency of natural cosmetic emulsion systems with the addition of vegetable oils
Provedor de dados: 60
Autores: Pavlačková,Jana; Kovacsová,Klára; Radiměřský,Petr; Egner,Pavlína; Sedlaříková,Jana; Mokrejš,Pavel.
The aim of the paper is to test stability and biophysical properties of hydrophilic and lipophilic emulsions with selected vegetable seed oils: Limnanthes alba, Prunus amygdalus dulcis, Cannabis sativa, Rosa rubiginosa and Hellianthus annuus. Biophysical properties of emulsions are investigated in vivo using non-invasive instrumental methods (corneometry, tewametry and pH) in a group of 12 healthy women volunteers. Their stability profiles (colour, phase separation and centrifugation) under various temperatures (9, 25, 37 and 57 °C) and storage time (24 hours, 2, 7, 14, 21 and 28 days) were monitored. The moisturising activities of the emulsions supplemented with various oils were comparable. The lipophilic emulsions showed a better ability to improve the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetable oil/emulsion/stability; Skin/hydration; TEWL.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300624

Antibiotic microbial assay using kinetic-reading microplate system
Provedor de dados: 60
Autores: Lourenço,Felipe Rebello; Pinto,Terezinha de Jesus Andreoli.
The aim of this study was to determine the optimal experimental conditions to develop a methodology for microbiological assay of apramycin employing microplate and kinetic reading mode, and to validate the developed method, through evaluation of parameters of selectivity, linearity, linear range, limits of detection and quantification, accuracy and precision. The turbidimetric assay principle is simple: the test solution is added to a suspension of test microorganism in culture media, the mixture is incubated under appropriate conditions and the microbial growth is measured by photometric reading. Microplate with kinetic reading mode employed in antibiotic assay is of considerable interest since it allows reduction of material and analysis time and enables...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics/microbial assay; Apramycin/microbial assay; Apramycin sulfate; Turbidimetric assay/medicines analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300015

Pharmacists and their new and important conquest!
Provedor de dados: 60
Tipo: Info:eu-repo/semantics/other
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400001

Fulminant hepatic failure caused by herbal supplies
Provedor de dados: 60
Autores: Santos Júnior,Genário Oliveira; Ferreira Filho,Raymundo Paraná; Foulon,Liana Machado de Codes; Noblat,Lúcia de Araújo Costa Beisl.
The consumption of botanicals for therapeutic purposes has increased significantly in recent years. Drug-induced liver disease (DILI) is a frequent cause of acute liver injury, around 50% in the United States, and about 1% is secondary to the use of phytotherapeuticals and herbal supplies. Ruellia bahiensis, a plant species of the Acanthaceae family, is a tropical plant distributed in Northeastern Brazil. In folk medicine in the state of Bahia, the species is known as “mãe-boa” and is commonly used. L.S.S, a 23-year old, female, patient was admitted at University Hospital of Bahia-Brazil with signs and symptoms of acute hepatitis. She had made daily use of an herbal supply popularly known as “mãe-boa” for at least two years prescribed by a physician....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver failure/study; Liver transplantation; Acute; Herbal Medicine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300612

Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells
Provedor de dados: 60
Autores: Wei,Dai; Yun,LI Shi; Dejun,Xiao; Cong,Liu; He,Jin-Hua; Yan,Lin.
To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin/ pharmacology LncRNA-MALAT1 RNA Small Interfering/ drug effects Colon cancer Invasion Migration Colonic Neoplasms/ prevention &amp;amp; Control Colonic Neoplasms/ drug therapy Neoplasm Invasiveness/ prevention &amp;amp; Control Cell Migration Inhibition/ drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100512

Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria)
Provedor de dados: 60
Autores: Nassima,Boumghar; Nassima,Behidj; Riadh,Ksouri.
The interest of this work is the discovery of new antimicrobial agents of plant origin to inhibit the formation of microbial biofilms. The present research was conducted to identify and quantify the phenolic compounds extracted from Populus nigra and Populus alba buds harvested in the area of Tizi-Ouzou (Algeria), and to evaluate their antimicrobial and antibiofilm activity. High performance liquid chromatography (HPLC) was performed to identify the phenolic compounds in the ethyl acetate fraction of P. nigra and the methanolic extracts of P. nigra and P. alba. The antimicrobial activity of the crude extracts and the fractions of these two species was tested against 11 microorganisms, using the disk diffusion method, while the antibiofilm effect of certain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Populus nigra; Populus alba; HPLC; Polyphenols; Antimicrobial activity; Antibiofilm activity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100563

Decades of research in drug targeting using gastroretentive drug delivery systems for antihypertensive therapy
Provedor de dados: 60
Autores: Porwal,Amit; Dwivedi,Harinath; Pathak,Kamla.
ABSTRACT The limitations in absorption of drugs with narrow absorption window, or those unstable in the intestinal pH or those exhibiting low solubility at high pH are primary candidates for gastroretentive drug delivery systems (GRDDS). The delivery system has been widely explored for its commercial potential for a wide variety of therapeutic agents. GRDDS offer clinical therapeutics for acute and chronic management. Hypertension is a chronic disease that requires long term treatment and its management by patient compliant dosage forms would be clinically useful. Antihypertensives belonging to different classes have proved good candidates for the formulation of GRDDS. The review aims to discuss various GRDDS researched for antihypertensive drugs to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antihypertensive drugs; Gastroretention delivery systems/absorption; Floating systems; Expanding and swelling systems; Bio/mucoadhesive systems.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300401

Pharmaceutical development of tablets containing a spray-dried optimized extract from Lippia origanoides H. B. K.: influence of excipients and toxicological assessment
Provedor de dados: 60
Autores: Coelho,Angélica Gomes; Lima Neto,José de Sousa; Moura,Arkellau Kenned Silva; Morais,Ilmara Cecilia Pinheiro da Silva; Cunha,Francisco Valmor Macedo; Melo-Neto,Bernardo; Medeiros,Maria das Graças Freire de; Vasconcelos,Eilika Andreia Feitosa; Arcanjo,Daniel Dias Rufino; Nunes,Lívio Cesar Cunha; Citó,Antônia Maria das Graças Lopes.
ABSTRACT Lippia origanoides is a honey shrub which has showed hypotensive potential assessed by in vivo studies. The aim of this work is the development of a pharmaceutical formulation composed by an optimized extract obtained from aerial parts of L. origanoides. The quantification of the naringenin marker in the dry extract and tablets developed was performed, as well as the assessment of the oral acute toxicity in rats. The hydroalcoholic extract of L. origanoides was spray-dried with the addition of colloidal silicon dioxide (Lo-HAE/CSD), and then applied in the preparation of eight different lots of tablets. The influence of the diluent (cellulose or babassu mesocarp), the presence of binder, and the percentage of lubricant, as well as organoleptic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lippia origanoides/pharmaceutical formulation/toxicity/; Babassu mesocarp; Factorial planning; Tablets.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200619

Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles
Provedor de dados: 60
Autores: Mattos,Ana Cristina de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.
The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 µg/mL (r = 0.9997). The precision (intra- and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluorouracil/determination; Nanoparticles; High performance liquid chromatography/quantitative analysis; High performance liquid chromatography/method validation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100013

Bioanalytical method development and validation for determination of metoprolol tartarate and hydrochlorothiazide using HPTLC in human plasma
Provedor de dados: 60
Autores: Rote,Ambadas Ranganath; Sonavane,Poonam Ramdas.
A simple, sensitive, rapid and economic chromatographic method has been developed for determination of metoprolol tartarate and hydrochlorothiazide in human plasma using paracetamol as an internal standard. The analytical technique used for method development was high-performance thin-layer chromatography. HPTLC Camag with precoated silica gel Plate 60F254 (20 cm×10 cm) at 250 µm thicknesses (E. Merck, Darmstadt, Germany) was used as the stationary phase. The mobile phase used consisted of chloroform: methanol: ammonia (9:1:0.5v/v/v). Densitometric analysis was carried out at a wavelength of 239 nm. The rf values for hydrochlorothiazide, paracetamol and metoprolol tartarate were 0.13±0.04, 0.28±0.05, 0.48±0.04, respectively. Plasma samples were extracted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance thin layer chromatography/quantitative analysis; Metoprolol tartarate/determination in plasma; Hydrochlorothiazide/determination in plasma; Human plasma/quantitative analysis; Protein precipitation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400025

Flow-injection spectrophotometric determination of captopril in pharmaceutical formulations using a new solid-phase reactor containing AgSCN immobilized in a polyurethane resin
Provedor de dados: 60
Autores: Vicentini,Fernando Campanhã; Suarez,Willian Toito; Cavalheiro,Éder Tadeu Gomes; Fatibello-Filho,Orlando.
A simple flow-injection analysis procedure was developed for determining captopril in pharmaceutical formulations employing a novel solid-phase reactor containing silver thiocyanate immobilized in a castor oil derivative polyurethane resin. The method was based on silver mercaptide formation between the captopril and Ag(I) in the solid-phase reactor. During such a reaction, the SCN- anion was released and reacted with Fe3+, which generated the FeSCN2+ complex that was continuously monitored at 480 nm. The analytical curve was linear in the captopril concentration range from 3.0 × 10-4 mol L-1 to 1.1 × 10-3 mol L-1 with a detection limit of 8.0 × 10-5 mol L-1. Recoveries between 97.5% and 103% and a relative standard deviation of 2% for a solution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgSCN; Captopril/determination; Flow-injection; Polyurethane resin; Solid-phase reactor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200016

Development and validation of HPLC method for analysis of dexamethasone acetate in microemulsions
Provedor de dados: 60
Autores: Urban,Maria Cristina Cocenza; Mainardes,Rubiana Mara; Gremião,Maria Palmira Daflon.
A simple, rapid, accurate and sensitive method was developed for quantitative analysis of dexamethasone acetate in microemulsions using high performance liquid chromatography (HPLC) with UV detection. The chromatography parameters were stainless steel Lichrospher 100 RP-18 column (250 mm x 4 mm i.d., 5 μm particle size), at 30 ± 2 ºC. The isocratic mobile phase was methanol:water (65:35; v/v) at a flow rate of at 1.0 mL.min-1. The determinations were performed using UV-Vis detector set at 239 nm. Samples were prepared with methanol and the volume injected was 20 μL. The analytical curve was linear (r² 0.9995) over a wide concentration range (2.0-30.0 μg.mL-1). The presence of components of the microemulsion did not interfere in the results of the analysis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dexamethasone acetate/determination; Microemulsions/quantitative analysis; High performance liquid chromatography/quantitative analysis; Analytical method/validation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100010

Comparative in vitro antioxidant potential of different parts of Ipomoea asarifolia, Roemer &amp;amp; Schultes, Guiera senegalensis, J. F. Gmel and Anisopus mannii N. E. Brown
Provedor de dados: 60
Autores: Ene-OjoAtawodi,Sunday; Onaolapo,Gboyega Suleiman.
In this study, the radical scavenging capacity of Guiera senegalensis, Ipomoea asarifolia and Anisopus mannii were compared. The ascorbic acid equivalent of the stem bark (2.1 mM), leaves (2.05 mM) and root barks (1.89 mM) of Guiera senegalesis are much higher than any part of Ipomoea asarifolia or Anisopus manii. The stem bark, leaves and root bark of Guiera Senegalensis had IC50 of 15.4, 20.0 and 17.0 µL respectively, while corresponding parts of Ipomoea asarifolia showed IC50 of 50, 42 and 65 µL, respectively. These results suggest that Guiera senegalensis and Ipomoea asarifolia possess significant antioxidant capacities to warrant further detailed studies on the role of this property in their therapeutic effects.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipomoea asarifolia/antioxidant potential; Guiera senegalensis/antioxidant potential; Anisopus mannii/antioxidant potential.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200011

Use of the Ogawa-Kudoh method to isolate mycobacteria in a tuberculosis reference laboratory in northwestern Paraná, Brazil
Provedor de dados: 60
Autores: Silva,Fernanda Schaefer Borges da; Castilho,Aline Lemes; Maltempe,Flaviane Granero; Pina,Rosangela Zampieri; Takao,Elisa Keiko Hirayama; Siqueira,Vera Lúcia Dias; Cardoso,Rosilene Fressatti; Caleffi-Ferracioli,Katiany Rizzieri.
Culturing is the gold standard method for confirming a diagnosis of tuberculosis (TB). The Brazilian Ministry of Health recently proposed the use of the Ogawa-Kudoh method for sputa cultures to detect Mycobacterium tuberculosis. The aim of the present study was to evaluate 8 years of using the Ogawa-Kudoh method in a TB reference laboratory in northwestern Paraná, Brazil. The present study consisted of a retrospective analysis of sputa cultures records for the detection of mycobacteria using the Ogawa-Kudoh method in the Laboratory of Medical Bacteriology, Laboratory of Teaching and Research in Clinical Analysis (LEPAC), State University of Maringá, from July 2003 to September 2011. The following variables were analyzed: Ziehl Neelsen (Z-N) smears and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis/detection; Ogawa-Kudoh method/evaluation; Tuberculosis/diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300018

Design and optimization of novel in situ gel of mercaptopurine for sustained drug delivery
Provedor de dados: 60
Autores: Nagendra,R.; Pai,Roopa S.; Singh,Gurinder.
Mercaptopurine is a purine antagonist, belonging to the class of antimetabolites. Its oral absorption is erratic and variable throughout GIT, with bioavailability of 5-37% and belongs to Biopharmaceutical Classification System (BCS) class IV. The focus of the present study was to improve solubility of mercaptopurine and to release the drug uniformly throughout the GIT by formulating into a novel in situ gel tablet. By in vitro swelling studies, xanthan gum was selected as the best gelling polymer and the tablets were prepared by direct compression. Sodium chloride was used as a release modifier to improve the release of drug from the tablet. A 32 full factorial design was applied to optimize the percentage of xanthan gum and sodium chloride to get desired...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercaptopurine/solubility; Mercaptopurine/drug delivery; Xanthan gum/use; Tablets/in situ gel.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100011

The importance of B cells in the development of preventive and therapeutical approaches against Dengue, Zika and Chikungunya viral infections
Provedor de dados: 60
Autores: Silveira,Eduardo L. V.
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100001

Dual effect of Algerian propolis on lung cancer: antitumor and chemopreventive effects involving antioxidant activity
Provedor de dados: 60
Autores: Brihoum,Hadjer; Maiza,Mhamed; Sahali,Hafida; Boulmeltout,Malika; Barratt,Gillian; Benguedouar,Lamia; Lahouel,Mesbah.
Abstract The purpose of our study was to divulge the antiproliferative effect of an ethanolic extract of Algerian propolis (EEP) in the human lung adenocarcinoma cell line (A549) and reveal the chemopreventive role against benzo(a)pyrene-induced lung carcinogenesis in albino Wistar rats. Cytotoxicity of EEP was evaluated using the MTT assay and cell adhesion in A549 cells. Moreover, rats were given 25 mg/kg of propolis for 5 days before induction of experimental lung cancer by a single intraperitoneal dose of 200 mg/kg benzo(a)pyrene. Body weight, lung weight, lipid peroxidation, marker enzymes, and enzymatic and non-enzymatic antioxidants were estimated. The EEP demonstrated an inhibitory effect on proliferation of A549 at 24 and 72 hours in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Algerian propolis/effects; Benzo(a)pyrene; Chemoprevention; Lung cancer; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100616

Encapsulation of flaxseed oil extract in alginate-salep system by ionic gelation
Provedor de dados: 60
Autores: Wissam,Zam; Samer,Housheh.
The objective of the current study was to encapsulate flaxseed oil extracted from finely grounded flaxseed powder and determined for its fatty acids content by GC/FID. Ionotropic gelation method was used to form beads containing 40% flaxseed oil with vitamin E being used as an antioxidant agent. The study employed calcium chloride solution as crosslinking agent with a combination of sodium alginate and salep. The effect of polymer concentrations and calcium chloride concentration on the morphology, entrapment efficiency and oil release was studied. The encapsulation efficiency reached 93.46 ± 0.064% using 0.6% of salep and 2% sodium alginate with 0.3M calcium chloride gelling solution. In SGF medium, the matrix released only about 28.56% of the entrapped...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flaxseed oil; Sodium alginate; Salep; Beads.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100578

Association of hypertension and dyslipidaemia with increasing obesity in patients with Type 2 Diabetes Mellitus
Provedor de dados: 60
Autores: Saleem,Zikria; Saeed,Hamid; Khan,Zohaib Abbas; Khan,Muhammad Imran Hassan; Hashmi,Furqan Khurshid; Islam,Muhammad; Bashir,Afzaal; Sadeeqa,Saleha.
The study was performed to estimate the association of hypertension and dyslipidaemia with increasing body weight and obesity in Type II diabetics of Lahore, Pakistan. An observational study was conducted by enrolling 2708 obese diabetics from four diabetes care centres of Lahore, Pakistan. Data was collected for a period of 7 months. Associations were estimated using chi-square, binary and multinomial logistic regression. Data suggested that blood pressure, systolic and diastolic, exhibited continual increase with increasing body weight and obesity class in diabetes patients with 41.8% increase in the prevalence of hypertension in obesity class III subjects (OR; 1.91, p=0.02). Likewise, triglycerides and total cholesterol exhibited continual increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type II Diabetes Mellitus; Dyslipidaemia; Obesity; HbA1c; Lahore/Pakistan.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100503

Screening of mucoadhesive vaginal gel formulations
Provedor de dados: 60
Autores: Andrade,Ana Ochoa; Parente,María Emma; Ares,Gastón.
Rational design of vaginal drug delivery formulations requires special attention to vehicle properties that optimize vaginal coating and retention. The aim of the present work was to perform a screening of mucoadhesive vaginal gels formulated with carbomer or carrageenan in binary combination with a second polymer (carbomer, guar or xanthan gum). The gels were characterised using in vitroadhesion, spreadability and leakage potential studies, as well as rheological measurements (stress and frequency sweep tests) and the effect of dilution with simulated vaginal fluid (SVF) on spreadability. Results were analysed using analysis of variance and multiple factor analysis. The combination of polymers enhanced adhesion of both primary gelling agents, carbomer and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucoadhesive vaginal gels/screening; Xanthan gum/use/vaginal products; Mucoadhesion; Vaginal products/rheology; Vaginal products/spreadability; Natural products/use/vaginal gels.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400931

Preparation and physicochemical characterization of meloxicam orally fast disintegration tablet using its solid dispersion
Provedor de dados: 60
Autores: Shoormeij,Zahra; Taheri,Azade; Homayouni,Alireza.
ABSTRACT Meloxicam (MLX) is a non-steroidal, anti-inflammatory drug that is prescribed in the treatment of rheumatoid arthritis and osteoarthritis. MLX is practically insoluble in water and exhibits a slow onset of action. In this study, MLX solid dispersions (MLX SDs) were prepared to improve the water solubility of this poorly water-soluble drug. Then orally disintegrating tablets (ODT) of MLX were developed using MLX SD to decrease the onset of action of this drug. MLX, poloxamer 188, and crospovidone of different ratios were melted in molten poloxamer 188 as a hydrophilic carrier. The optimum SD with the highest saturation solubility in water (13.09±0.34 microgram/mL) consisting of MLX: poloxamer 188: crospovidone in the ratio of 1:2:0 was used for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meloxicam/physicochemical characterization; Meloxicam/onset of action; Solubility.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400606

Formulation, in vitro drug release and in vivo human X-ray investigation of polysaccharide based drug delivery systems for targeting 5-fluorouracil to the colon
Provedor de dados: 60
Autores: Chickpetty,Sidramappa Mallikarjun; Raga,Baswaraj Veerappa.
The purpose of this research study was to develop 5-fluorouracil compression coated tablets by using biodegradable polysaccharide polymer locust bean gum (LBG) and hydroxyl propyl methyl cellulose (HPMC) as coating materials. The fast disintegrating core tablets containing 50 mg of 5-fluorouracil were compression coated with LBG and HPMC in different ratios (8:1, 7:2 and 6:3) with a coat weight of 300, 400 and 500 mg. In vitro dissolution data indicated that the formulation (CLH63) with a coat weight of 500 mg containing LBG and HPMC in the ratio 6:3 gave the best release profile (0% in first 5 hour and 96.18% in 24 hours). DSC and FTIR results indicated no possibility of interaction between drug and polymers or other excipients. In vivo human X-ray...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compression coated tablet; Locust bean gum; Colon targeting; 5-fluorouracil; Colon cancer and X-ray.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200008

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages
Provedor de dados: 60
Autores: Benetti,Thalyta Marina; Monteiro,Cristina Leise Bastos; Beux,Márcia Regina; Abrahão,Wanda Moscalewski.
The aim of this study was to determine the prevalence of Listeria sp. in refrigerated sausages, and to compare the performance of the selective plating media employed in the ISO 11290-1 method (PALCAM and Oxford agars) with chromogenic agars (Chromogenic Listeria agars CM 1080 (OCLA) and CM 1084). The prevalence of Listeria sp. detected was 52.9%, comprising 13.7% L. monocytogenes strains. The efficacy of the four agars for the isolation of L. monocytogenes proved to be satisfactory. Despite differences in composition of the chromogenic media assessed, these disparities did not affect concordance among results. However, PALCAM agar was shown to suppress other microorganisms more effectively, being more applicable for detecting Listeria strains present in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria sp./detection; Listeria sp./meat products; Meat products/qualitative analysis; Refrigerated sausages/qualitative analysis; PALCAM Agar; Oxford Agar; Chromogenic Agar.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400011

4-Nerolidylcatechol induces autophagy in human glioblastoma cells
Provedor de dados: 60
Autores: Massaro,Renato Ramos; Brohem,Carla Abdo; Almeida,Rebeca Leite de; Rivelli,Diogo Pineda; Miyake,Juliano Andreoli; Colquhoun,Alison; Barros,Silvia Berlanga de Moraes; Maria-Engler,Silvya Stuchi.
ABSTRACT Gliomas account for the majority of primary malignant brain tumors and present invasive behavior into adjacent healthy tissue. While 4-NC had previously shown to induce apoptotic cell death in a melanoma model, for the glioma model described in this paper 4-NC is cytotoxic for the cells with the induction of the autophagic pathway. Trypan blue exclusion assay showed that 4-NC was cytotoxic in a dose-dependent manner for A172 and T98G cell lines. IC10 and IC50 values were at 32 µM and 41 µM for A172 and T98G respectively. Inhibition of cell proliferation was observed by total cell counts and by cell cycle analysis by flow cytometry, with cell cycle arrest of A172 and T98G cell lines respectively in the G1/G0 and S phases of the cell cycle. 4-NC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-Nerolidylcatechol; Glioblastoma; Cytotoxicity; Autophagy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300618

Avaliação da estabilidade e atividade antioxidante de uma emulsão base não-iônica contendo resveratrol
Provedor de dados: 60
Autores: Lange,Marcela Kist; Heberlé,Graziela; Milão,Denise.
Vários são os fatores que podem ocasionar a instabilidade de uma emulsão, destacando-se a oxidação, reação prevenida pelo emprego de antioxidantes. O butil-hidróxi-tolueno (BHT) tem sido um dos antioxidantes sintéticos mais utilizados em formulações cosméticas, porém, a busca da indústria farmacêutica e cosmética pelo emprego de produtos de origem natural tem sido cada vez maior. Visto isso, o objetivo do presente trabalho foi a incorporação do resveratrol, um composto fenólico encontrado principalmente em uvas bem como em vinhos tintos, em uma emulsão base não-iônica para avaliação do perfil de estabilidade e atividade antioxidante em comparação a uma emulsão base não-iônica contendo o BHT. O perfil de estabilidade foi analisado pela observação das...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emulsões/estabilidade; Antioxidantes; Butil-hidróxi-tolueno; Resveratrol.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100018

Effects of polyethylene glycols on intestinal efflux pump expression and activity in Caco-2 cells
Provedor de dados: 60
Autores: Hodaei,Darya; Baradaran,Behzad; Valizadeh,Hadi; Zakeri-Milani,Parvin.
The present study was planned to investigate the influence of polyethylene glycols (PEGs) on the activity and expression of P-glycoprotein (P-gp). Sub-toxic concentrations of PEGs in Caco-2 cells were determined using the MTT test assay. Then the measurement of Rhodamine-123 (Rho-123) uptake, a P-gp fluorescence substrate, in Caco-2 cells confronting PEG 400 (1% and 2% w/v), PEG 4000 (2% and 4% w/v), PEG 6000 (2% and 4% w/v), PEG 10000 (2% and 4% w/v), PEG 15000 (1% and 2% w/v), and PEG 35000 (2% and 4% w/v) overnight was taken to elucidate whether non-toxic concentrations of PEGs are able to impact P-gp activity. Furthermore, western blotting was carried out to investigate P-gp protein expression. The results showed that PEG 400 at concentrations of 1%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-glycoprotein/activity; P-glycoprotein/expression; Caco-2; Rhodamine-123; Excipients; Drugs/bioavailability; Polyethylene glycol/effects.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300745

Characterization of gliclazide-polyethylene glycol solid dispersion and its effect on dissolution
Provedor de dados: 60
Autores: Patil,Moreshwar Pandharinath; Gaikwad,Naresh Janardan.
The present study was initiated with the objective of studying the in vitro dissolution behavior of gliclazide from its solid dispersion with polyethylene glycol 6000. In this work, a solid dispersion of gliclazide with polyethylene glycol was prepared by the fusion method. In vitro dissolution study of gliclazide, its physical mixture and solid dispersion were carried out to demonstrate the effect of PEG 6000. Analytical techniques of FT-IR spectroscopy, differential scanning calorimetry and X-ray diffractometry were used to characterize the drug in the physical mixtures and solid dispersions. The dissolution studies of solid dispersion and physical mixture showed greater improvement compared to that of the pure drug. The mechanisms for increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gliclazide; Polyethylene glycol 6000; Solid dispersion; In vitro dissolution.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100020

Inverted sugar syrup attained from sucrose hydrolysis using a membrane reactor
Provedor de dados: 60
Autores: Tomotani,Ester Junko; Vitolo,Michele.
Invertase, whether adsorbed on styrene-divinylbenzene copolymers or otherwise, was used for continuous sucrose hydrolysis using a cell-type membrane reactor (CTMR), coupled with an ultra (UF-100kDa), or a microfiltration (MF- pore diameter of 5 µm) membrane. In all tests, the pH (5.5), temperature (30 ºC), reaction volume (10 mL) and agitation (100 rpm) were set constant; whereas, variable parameters were: feeding rate (0.4, 0.8 and 1.6 h-1), inlet sucrose concentration (2.5, 6.5, 50 and 100 mM) and enzyme/resin ratio (1.64 mg or 3.28 mg of protein per 25, 50 or 100 mg of resin). The best result (yield of 100%, steady-state duration over 20h and specific reaction rate over 243 x 10-3 mmol/h.mE) was obtained when insoluble invertase (1.64 mg protein/100 mg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sucrose; Invert sugar; Membrane reactor.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300022

Investigation on the in vitro antioxidant capacity of methanol extract, fractions and flavones from Oroxylum indicum Linn bark
Provedor de dados: 60
Autores: Trang,Do Hoang Thu; Son,Hoang Le; Trung,Phung Van.
ABSTRACT Antioxidants from natural sources hold high values regarding their indispensible roles in the development of nutraceuticals, pharmaceuticals and cosmetic products. Oroxylum indicum L. is a common medicinal plant with a wide range of therapeutic properties, including a notable antioxidant potency that was reported, yet has not been subjected to more detailed studies. The present study evaluated the potency of Oroxylum indicum methanol stem bark extract, along with its hexane, ethyl acetate, methanol fractions, three flavones including baicalein, oroxylin A and chrysin using DPPH assay. In terms of IC50 values, the crude extract (65,48 μg/mL) exhibited moderate inhibitory activity which was as half potent as that of its ethyl acetate fraction (32,94...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oroxylum indicum; Flavones; Methanol/extract; DPPH assay; Antioxidants; Inhibitory Concentration 50; Bignoniaceae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100619

Evolution of potential biomarkers of acute muscle injury after physical exercise
Provedor de dados: 60
Autores: Chielle,Eduardo Ottobelle; Granella,Lucilene Wildner; Maziero,Jorlana Stacke; Vidigal,Tiago Mateus Andrade; Mallmann,Bárbara Lidiane Kummer; Karal,Jaison.
Skeletal muscle injury is a frequent event and diagnosis using the classical blood markers sometimes produces unsatisfactory results. Therefore, objective of the study was to detect new biomarkers in plasma, saliva and urine in response to acute muscle damage induced by physical exercise. A cross-sectional study was conducted with 27 American football players. Before the physical exercises (T0), 60 minutes (T1) and 24 hours (T2) after physical exercise, was determined the clinical, biochemical and molecular parameters, including ADA, TBARS, leukocytes, lymphocytes and comet assay. The serum ADA was significantly higher in T1 and T2, in the urine there was a significant increase in T1, in the saliva there was no significant differences. There was an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers/serum; Biomarkers/saliva; Biomarkers/urine; Exercise; Athletes; Athletic injuries; Adenosine deaminase/analysis; DNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100549

Residual solvent determination by head space gas chromatography with flame ionization detector in omeprazole API
Provedor de dados: 60
Autores: Pandey,Saurabh; Pandey,Preeti; Kumar,Raj; Singh,Narendra Pal.
Residual solvents in pharmaceutical samples are monitored using gas chromatography with head space. Based on good manufacturing practices, measuring residual solvents is mandatory for the release testing of all active pharmaceutical ingredients (API). The analysis of residual organic solvents (methanol, acetone, cyclohexane, dichloromethane, toluene) in Omeprazole, an active pharmaceutical ingredient was investigated. Omeprazole is a potent reversible inhibitor of the gastric proton pump H+/K+-ATPase. The Head space gas chromatography (HSGC) method described in this investigation utilized a SPB TM-624, Supelco, 30 m long x 0.25 mm internal diameter, 1.4µm-thick column. Since Omeprazole is a thermally labile compound, the selection of the proper injector...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Headspace-gas chromatography; Omeprazole; Method validation; Residual solvents release testing.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200019

Antinociceptive local activity of 4-allyl-1-hydroxy-2-methoxybenzene (eugenol) by the formalin test: an anti-inflammatory effect
Provedor de dados: 60
Autores: Lugo-Lugo,Daniela Elizabeth; Pozos-Guillén,Amaury de Jesús; Zapata-Morales,Juan Ramón; Rodríguez-Chong,Antonio; Rangel-López,Azahel de Jesús; Saavedra-Leos,María Zenaida; Vértiz-Hernández,Angel Antonio.
Eugenol has been employed for decades as a condiment, an antimycotic, an antibacterial, an antiviral, and an antioxidant, and it is one of the natural analgesics most frequently utilized for pain and inflammation. Our objective was to determine the analgesic/anti-inflammatory effect of eugenol compared with diclofenac, naproxen, and tramadol using the formalin test. The formalin method was used in 6- to 10-week-old Wistar rats (weighing 250 g each) divided into six groups: saline (0.9%); formalin (5%); diclofenac (250 µg/kg); naproxen (400 µg/kg); tramadol (500 µg/kg), and eugenol (1,400 µg/kg), in the intraplantar part of the hind-end trunk of the rats, with n = 5 per group. Eugenol diminished 44.4% of nociceptive behavior in phase 1 and 48% in phase 2 (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenol/analgesic/anti-inflammatory effect; Diclofenac effect; Antinociception; Formalin test; Pain.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100536

Development of para rubber seed oil as the efficient makeup remover
Provedor de dados: 60
Autores: Lourith,Nattaya; Kanlayavattanakul,Mayuree.
The para rubber tree (Hevea basilensis) is an economically important tropical tree species that produces natural rubber, a valuable industrial raw material. Innovative products that are derived from by-products from the rubber production process, such as seeds, are important for the sustainability of the para rubber industry. Therefore we subjectively assessed para rubber seed oil for its makeup-removal efficacy and developed it into a makeup cleansing product with appropriate quality control measures for the cosmetic industry. The makeup-removal efficacy of para rubber seed oil was analyzed using a validated UV-Vis spectroscopy, which indicated that the oil removed 86.21 ± 2.48% of liquid foundation [relative standard deviation (RSD) = 2.88%]. Therefore,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Para rubber; Bio-oil; Color cosmetics; Makeup remover; Cleansing efficacy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100545

Prevalence of methicillin-resistant Staphylococcus aureus in a University Hospital in the South of Brazil
Provedor de dados: 60
Autores: Rodrigues,Mônica de Abreu; Gindri,Lívia; Silva,Aniélen Dutra da; Guex,Camille Gaube; Santos,Silvana Oliveira dos; Hörner,Rosmari.
Methicillin-resistant Staphylococcus aureus (MRSA) stand out as one of the main agents causing nosocomial and community infections. This retrospective study aimed to analyze the MRSA predominance in a university hospital in the south of Brazil and it was carried out for five years (from 2007 to 2011). 616 MRSA (33,3% of the total) were isolated and an important reduction in the MRSA predominance was observed along the study. Although it was registered a reduction in the MRSA predominance, male adult patients (41-70 years old), who were in the Medical Clinic and Adult ICU, had the highest infection rates and concerning MRSA isolates rates, these were higher in blood and tracheal aspirates. In conclusion, studies of this type are becoming relevant to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus/methicillin-resistant; Resistant pathogens; Methicillin; University hospitals/infection.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100035

In vitro characterization and in vivo performance of mefenamic acid-sodium diethyldithiocarbamate based liposomes
Provedor de dados: 60
Autores: Jarrar,Qais Bashir; Hakim,Muhammad Nazrul; Cheema,Manraj Singh; Zakaria,Zainul Amiruddin.
Mefenamic acid (MFA) is a hydrophobic drug with low dissolution rate. This study aimed to develop stable and reproducible aqueous formulations of MFA using liposomes as drug carriers. The drug entrapment, particles size and drug release profiles, and stability and reproducibility of the liposomes were determined. In addition, the maximum tolerated dose (MTD) was determined in rats via the oral and intraperitoneal routes of administration. Also, the anti-inflammatory efficacy of these liposomes was evaluated using carrageenan-induced paw edema model in rats. MFA-DDC based liposomes demonstrated a drug entrapment efficacy of 93.6%, particles size of 170.9 nm, and polydispersity index of 0.24 which were not statistically affected when stored in room and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mefenamic acid; Liposomes-characterization; Sodium diethyldithiocarbamate; Extrapyramidal symptoms; Carrageenan.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100553

Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography
Provedor de dados: 60
Autores: Székely-Szentmiklósi,Blanka; Hancu,Gabriel; Székely-Szentmiklósi,István; Kovács,Béla; Kelemen,Hajnal.
Abstract A rapid and sensitive micellar electrokinetic capillary chromatography method with UV photodiode-array detection was developed for the simultaneous determination of atorvastatin and ezetimibe in fixed dose drug combination. Experimental conditions such as buffer concentration and pH, surfactant concentration, system temperature, applied voltage, injection parameters were optimized in order to improve the efficiency of the separation. The best results were obtained when using fused silica capillary (48 cm length X 50 µm ID) and 25 mM borate buffer electrolyte at pH 9.3 containing 25 mM SDS, + 30 kV applied voltage, 20 ºC system temperature. The separation was achieved in approximately 2 minutes, with a resolution of 7.02, the order of migration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micellar Electrokinetic Capillary Chromatography; Fixed Dose Drug Combination; Atorvastatin; Ezetimibe.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100626

Effect of metformin on semen quality
Provedor de dados: 60
Autores: Banihani,Saleem Ali.
ABSTRACT Various studies have linked metformin, a universally antidiabetic drug, with semen quality; however, such a direct link has not been established. This review systematically addresses and summarizes the effect of metformin on semen quality, particularly sperm function. We searched the MEDLINE electronic database for English articles and abstracts containing the key words 'metformin' and 'sperm', and relevant articles were reviewed. In summary, metformin appears to have improved and provided positive impact on sperm quality. This effect may be due to the ability of metformin to reduce oxidative stress and lipid peroxidation, enhance 5'-AMP activated protein kinase activity, and restore the normal levels of pituitary-gonadal hormones. However,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metformin/effects; Metformin/review; Metformin/semen quality; Sperm/study; Testosterone; Oxidative stress; 5'-AMP activated protein kinase.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400591

Enhancement of solubility and dissolution rate of poorly water soluble raloxifene using microwave induced fusion method
Provedor de dados: 60
Autores: Patil,Payal Hasmukhlal; Belgamwar,Veena Sailendra; Patil,Pratibha Ramratan; Surana,Sanjay Javerilal.
The objective of the present work was to enhance the solubility and dissolution rate of the drug raloxifene HCl (RLX), which is poorly soluble in water. The solubility of RLX was observed to increase with increasing concentration of hydroxypropyl methylcellulose (HPMC E5 LV). The optimized ratio for preparing a solid dispersion (SD) of RLX with HPMC E5 LV using the microwave-induced fusion method was 1:5 w/w. Microwave energy was used to prepare SDs. HPMC E5 LV was used as a hydrophilic carrier to enhance the solubility and dissolution rate of RLX. After microwave treatment, the drug and hydrophilic polymer are fused together, and the drug is converted from the crystalline form into an amorphous form. This was confirmed through scanning electron microscopy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raloxifene HCl; Microwave-induced fusion method; Solid dispersion; Solubility enhancement; HPMC E5 LV.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300019

The effect of dissolution medium, rotation speed and compaction pressure on the intrinsic dissolution rate of amlodipine besylate, using the rotating disk method
Provedor de dados: 60
Autores: Giorgetti,Leandro; Issa,Michele Georges; Ferraz,Humberto Gomes.
The aim of this study was to evaluate the effect of dissolution medium, rotation speed and compaction pressure on the intrinsic dissolution rate (IDR) of the antihypertensive drug amlodipine besylate, using the rotating disk method. Accordingly, a fractional factorial design (33-1) was used, employing dissolution media (water, phosphate buffer pH 6.8 and HCl 0.1 M), rotation speed (50, 75 and 100 rpm), and compaction pressure (1000, 1500 and 2000 psi) as independent variables. The assays were randomized and statistically compared using the Statistica(r) 11 software program. Significance testing (ANOVA) indicated that the dissolution medium had a considerable impact on the IDR of amlodipine besylate. Analysis of the linear and quadratic components of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amlodipine besylate/intrinsic dissolution; Biopharmaceutical classification system; Drugs/experimental design; Antihypertensive drugs/Amlodipine besylate.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300513

Preparation of extemporaneous oral liquid in the hospital pharmacy
Provedor de dados: 60
Autores: Silva,Márcio Robert Mattos da; Dysars,Letícia Pereira; Santos,Elisabete Pereira dos; Ricci Júnior,Eduardo.
At the hospital, the pharmacist is constantly challenged to prepare extemporaneous solutions from tablets, capsules or drug powder for patients unable to swallow, such as pediatric, elderly and patients that use nasoenteric and nasogastric tubes. The preparation of extemporaneous solutions from capsules, tablets and drug powder requires stability studies analysis. This article is a bibliographic review of preparation of extemporaneous oral liquid from solid oral dosage forms used in clinical practice. The selected articles contain all the information regarding manipulation techniques, pharmaceutical excipients, packaging, storage conditions and results of stability studies above 90% performed by HPLC analysis. In addition, a situational analysis of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extemporaneous oral liquid; Stability; Hospital pharmacy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100703

Relationship between composition and organizational levels of nanostructured systems formed by Oleth 10 and PPG-5-Ceteth-20 for potential drug delivery
Provedor de dados: 60
Autores: Rissi,Nathalia Cristina; Guglielmi,Danila Aparecida Scatolin; Corrêa,Marcos Antônio; Chiavacci,Leila Aparecida.
In this paper, nanostructured systems were developed, with the aid of ternary phase diagrams, from two surfactants, of differing degrees of lipophilicity (PPG-5-Ceteth-20 and the Oleth 10) and two oil phases (oleic acid and isopropyl myristate). It was observed that there were differences between the four resulting phase diagrams in the physical properties of the systems they represent. Thus, due to the capacity of Oleth 10 (as surfactant) and oleic acid (as the oil phase) to reduce interfacial tension, large regions of translucent systems were seen on the diagrams produced by them. By polarized light microscopy, it was possible to identify the isotropic and anisotropic properties of these systems, which were confirmed by small-angle X-ray scattering...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ternary phase diagrams; Nanostructured systems; Small-angle X-ray scattering/profiles; Drugs/delivery systems.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300653

Synergistic effect of microRNA and albumin-bound nanoparticles for inhibition of glioblastoma cancer cell proliferation
Provedor de dados: 60
Autores: Shariatnasery,Maria; Irani,Shiva; Soleimani,Masoud; Goodarzi,Navid; Dinarvand,Rassoul.
The functional significance of upregulation miR-34a in combination with albumin-bound paclitaxel nanoparticles in U251 glioblastoma cell line has been evaluated. The MTT assay determined that miR-34a and albumin-bound paclitaxel nanoparticles can reduce cell viability, but the combination of both factors has a stronger effect on cell viability. The application of qRT-PCR has demonstrated that the transduction of miR-34a could lead to exogenous upregulation of miR-34a level and downregulation of SURVIVIN. Moreover, treatment of U251 cells with miR-34a and nanoparticles together considerably inhibit SURVIVIN expression compared to miR-34a and nanoparticles alone. Flow cytometry showed that upon miR-34a overexpression cell cycle arrested in G1 phase, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-34a; Glioblastoma; Albumin; Paclitaxel; Nanoparticles; Combination therapy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100535

Effects of Hypericum perforatum on turning behavior in an animal model of Parkinson's disease
Provedor de dados: 60
Autores: Vecchia,Débora Dalla; Schamne,Marissa Giovanna; Ferro,Marcelo Machado; Santos,Ana Flávia Chaves dos; Latyki,Camila Lupepsa; Lara,Danilo Vieira de; Ben,Juliana; Moreira,Eduardo Luiz; Prediger,Rui Daniel; Miyoshi,Edmar.
Parkinson's disease (PD) is an age-related neurodegenerative disorder characterized by the slow and progressive death of dopaminergic neurons in the (substantia nigra pars compact). Hypericum perforatum (H. perforatum) is a plant widely used as an antidepressant, that also presents antioxidant and anti-inflammatory properties. We evaluated the effects of H. perforatum on the turning behavior of rats submitted to a unilateral administration of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle as an animal model of PD. The animals were treated with H. perforatum (100, 200, or 400 mg/kg, v.o.) for 35 consecutive days (from the 28th day before surgery to the 7th day after). The turning behavior was evaluated at 7, 14 and 21 days after the surgery,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson's disease/treatment/experimental study; H. perforatum/phytotherapy/neuroprotective effect; Turning behavior; 6-OHDA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100111

Evaluation of oxidative stability of mayonnaise containing poly ε- caprolactone nanoparticles loaded with thyme essential oil
Provedor de dados: 60
Autores: Passos,Rhaysa Beatriz dos; Bazzo,Giovana Carolina; Almeida,Aline da Rosa; Noronha,Carolina Montanheiro; Barreto,Pedro Luiz Manique.
Suspensions of poly ε-caprolactone (PCL) nanoparticles loaded with thyme essential oil were prepared as a natural antioxidant in mayonnaise. Mean particle size was 204.9 ± 2.7 and 240.0 ± 5.5 nm respectively for nanoparticles prepared with PCL alone (NP-C) and for those loaded with thyme essential oil (NP-T). The polydispersity index indicated a homogeneous distribution of all particles, with no significant difference between NP-C and NP-T samples. The nanoparticles showed a large negative charge evidenced by zeta potential rates, indicating high physical stability. The use of PCL as a polymer provided high encapsulation efficiency for thyme essential oil (91.15 ± 2.12 %). DPPH (2,2-diphenyl-1-picrylhydrazyl) method determined IC50 rates were 476.4 ± 33.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles Poly ε-caprolactone Thyme Essential Oil Mayonnaise Oxidative stability Antioxidants/adverse effects Thymus Plant Aromatherapy/utilization Butylated Hydroxytoluene/analysis Oxidation/prevention &amp;amp; Control.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100505

Up-regulation of human immune system function by Donkey’s Milk
Provedor de dados: 60
Autores: Taghiloo,Saeid; Allahmoradi,Esmaeil; Sadeghian-Kiadehi,Seyedeh Forough; Omrani-Nava,Versa; Nazar,Eisa; Ebrahimzadeh,Mohammad Ali.
Donkey’s milk represents a good alternative to human milk because of its chemical characteristics similar to those of human’s. In present study, the pro- and anti-inflammatory effects of donkey’s milk were evaluated on peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from 12 young and 12 aged normal subjects. PBMCs were cultured with or without the optimal and non-cytotoxic dose of pasteurized donkey’s milk, and polymyxin B was used to inhibit the possible endotoxin contamination. Following 18 hours incubation, culture supernatants were harvested to measure the secreted Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Interleukin-10 (IL-10) by ELISA. Donkey’s milk significantly increased TNF-α (p= 0.01), IL-8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Donkey’s milk; ELISA; Immune system; Aged subject; Cytokine.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100607

Influence of particle size on appearance and in vitro efficacy of sunscreens
Provedor de dados: 60
Autores: Silva,Débora Granemann e; Sarruf,Fernanda Daud; Oliveira,Larissa Carolina Diniz de; Arêas,Elizabeth Pinheiro Gomes; Kaneko,Telma Mary; Consiglieri,Vladi Olga; Velasco,Maria Valéria Robles; Baby,André Rolim.
Nanotechnology applies to diverse sectors of science. In cosmetic area, investments have strengthened the idea that nanoproducts provide innumerable benefits to consumers. Extreme exposition to solar light can cause undesirable effects, thus, adding UV filters in cosmetic products are often used as prevention. Ethylhexyl methoxycinnamate and benzophenone-3 are UV filters widely used in sunscreen formulations, this UV filters absorb UVB and UVA radiation, respectively. In this study, sunscreen formulations were developed as nano and macroemulsion, but composed by the same raw material. Nanoemulsion was obtained by phase inversion temperature method (PIT). Physical and functional properties were evaluated by visual analysis, particle size distribution and by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoemulsion; PIT method; Benzophenone-3; Ethylhexyl methoxycinnamate.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200007

Vegetable moisturizing raw material from “Caatinga” Brazilian biome: safety and efficacy evaluations of O/W cosmetic emulsions containing Kalanchoe brasiliensis extract
Provedor de dados: 60
Autores: Rodrigues,Rayllan de Oliveira; Damasceno,Gabriel Azevedo de Brito; Barreto,Stella Maria Andrade Gomes; Fernandes,Julia Morais; Telaprolu,Krishna Chaitanya; Rocha-Filho,Pedro Alves da; Soares,Luiz Alberto Lira; Ostrosky,Elissa Arantes; Sales,Valeria Soraya de Farias; Langassner,Silvana Maria Zucolotto; Ferrari,Márcio.
The purpose of this study was to evaluate the safety of Kalanchoe brasiliensis extract, followed by the development of an oil in water emulsion containing the K. brasiliensis leaves extract and evaluating its clinical moisturizing efficacy. The formulations containing sodium acrylates/ Beheneth-25 methacrylate Crosspolymer (and) hydrogenated polydecene (and) lauryl glucoside and 0.5% of extract were prepared. The extract was considered as non-irritating through skin irritant tests. The stability testing was carried out in different conditions for 90 days. The skin hydration was measured by capacitance measurement and transepidermal water loss using biophysical techniques. The results indicate that the formulation containing 0.5% of extract increased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmetics/products; Kalanchoe brasiliensis/extract/safety evaluation; Kalanchoe brasiliensis/moisturizing efficacy; Stability studies; Brazil/plants.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400624

Does Lycium europaeum leaf have antihyperglycemic, antihyperlipidemic and antioxidant effects
Provedor de dados: 60
Autores: Tej,Rabeb; Hamdi,Amel; Serairi Beji,Raja; Aidi Wannes,Wissem; Bettaieb Rebey,Iness; Oueslati,Samia; Ksouri,Riadh; Saidani Tounsi,Moufida; Lachaal,Mokhtar; Karray Bouraoui,Najoua.
The purpose of the present investigation is to assess, for the first time, the antidiabetic, antihyperlipidemic and antioxidant activities of Lycium europaeum extract in alloxan-induced diabetic rats. Diabetes was induced in adult male Wistar rats via a single subcutaneous alloxan injection (120 mg/kg). Lycium europaeum aqueous extract was orally administered at a dose of 20 mg/kg for 28 consecutive days. Serum concentrations of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG) were assayed at the end of the experimental period in all investigated groups. Antioxidant enzymes such as glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) were sought...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycium europaeum/extract/antihyperglycemia; Lycium europaeum/extract/antioxidant activity; Lycium europaeum/extract/antihyperlipidemia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100574

Evaluation of polyelectrolyte and emulsion covalent crosslink of chitosan for producing mesalasine loaded submicron particles
Provedor de dados: 60
Autores: Lacerda,Gabriel José Silveira; Piantino,Beatriz Lemos; Gonzaga,Edeilson Vitor; Naves,Valéria de Moura Leite; Pedreiro,Liliane Neves; Gremião,Maria Palmira Daflon; Pereira,Gislaine Ribeiro; Carvalho,Flávia Chiva.
This study evaluates various techniques for producing mesalamine (5ASA)-loaded particles employing chitosan as a biopolymer: (1) the polyelectrolyte complexation of chitosan with phthalate hypromelose (HP), (2) the chemical crosslinking of chitosan with genipin and (3) the water-in-oil emulsion method associated with chemical crosslinking with genipin. Systems were characterized by dynamic light scattering, zeta potential (ζ), powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR) and a drug release profile. Method (1) was efficiently produced unloaded nanoparticles (491 nm, PdI=0.26 and ζ = 23.2), but the conditions for chitosan and HP cross-linking enhanced the precipitation of 5ASA. Method (2) caused the degradation of the drug....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emulsion method; Polyelectrolyte crosslinking; Chemical cross-linking; Chitosan; Mesalazine.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100602

Standardization of method for determining glycosylated hemoglobin (Hb A1c) by cation exchange high performance liquid chromatography
Provedor de dados: 60
Autores: Antunes,Marina Venzon; Wagner,Sandrine Comparsi; Camargo,Joíza Lins; Linden,Rafael.
Hemoblobin A1c is the most important parameter for the monitoring of metabolic control of patients with diabetes mellitus. The purpose of this study was to adapt the Mono S method to a conventional HPLC system, allowing highly selective HbA1c determination without the acquisition of kits or the use of dedicated systems The results obtained were compared to the Tinaquant® immune turbidimetric method and the Bio-Rad Variant® chromatographic method. The developed method presented intra-study precision (C.V. %) of 1.39-3.65 and inter-study precision (C.V. %) of 2.80-3.02%. The determination coefficients among methods were: HPLC Mono S x Tinaquant®: r²: 0.9856 (n=60) and HPLC Mono S x HPLC Bio-Rad Variant®: r²: 0.9806 (n=16). A conversion equation between HPLC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography; Mono S/method; Hemoglobin/A1c; Hemoglobin/variants.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400007

Assessment of in vitro methodologies to determine topical and transdermal delivery of the flavonoid quercetin
Provedor de dados: 60
Autores: Vicentini,Fabiana Testa Moura de Carvalho; Georgetti,Sandra Regina; Bentley,Maria Vitória Lopes Badra; Fonseca,Maria José Vieira.
To be effective against the oxidative damages induced by UVB irradiation in the skin, the drug needs to release from the formulation in which it was incorporated and reach the skin layers where the ROS are generated. Thus, it is very important the development of a robust and sensitive methodology to extract and quantify in different skin layers the antioxidant agent delivered from topical formulations. Therefore, in the present work suitable methods to extract and quantify quercetin in skin samples and receptor phase after in vitro penetration studies were developed. The results demonstrated that the recovery from two different layers of skin, the SC and [E+D], using two different methods of quantification (DPPH• assay and HPLC, respectively), was 93.8 %...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Topical formulations/analysis; Quercetin/quantification; Quercetin/extraction; Quercetin/liberation; Skin penetration in vitro/study; High performance liquid chromatography; DPPH•.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200022

Hydrocortisone release from tablets based on bioresorbable poly(ether-ester-urethane)s
Provedor de dados: 60
Autores: Orozco-Castellanos,Luis Manuel; Marcos-Fernández,Angel; Alonso-Castro,Angel Josabad; González-García,Gerardo; Báez-García,José Eduardo; Rivera-Leyva,Julio Cesar; Zapata-Morales,Juan Ramón; Ruiz-Padilla,Alan Joel.
Abstract Bioresorbable linear poly(ether-ester-urethane)s with different hydrophilic characteristics were synthesized from triblock copolymers of poly(ε-caprolactone)-poly(ethylene oxide)-poly(ε-caprolactone) (PCL-PEO) as macrodiols, and L-lysine diisocyanate (LDI) or hexamethylenediisocyanate (HDI) were used as the required diisocyanates. Macrodiols were obtained by ring-opening polymerization (ROP) of ε-caprolactone (CL). Polyurethanes were synthesized by the reaction of the triblock copolymers with LDI or HDI in solution using stannous 2-ethylhexanoate as catalyst. Polyurethane tablets were fabricated and investigated as prospective drug delivery systems. The effect of the PEO content on the polymers' performance as drug carriers was evaluated. It was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioresorbable polyurethanes; Drug delivery; Hydrocortisone; Macrodiol.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100629

Evaluation of Prunus domestica gum as a novel tablet binder
Provedor de dados: 60
Autores: Rahim,Haroon; Khan,Mir Azam; Badshah,Amin; Chishti,Kamran Ahmad; Khan,Salimullah; Junaid,Muhammad.
To evaluate binding potential of Prunus domestica gum in tablets formulations. Six tablet batches (F-1B to F-6B) were prepared by wet granulation method, containing Avicel pH 101 as diluent, sodium diclofenac as model drug using 10, 15 and 20 mg of Prunus domestica gum as binder and PVP K30 was used as standard binder. Magnesium stearate was used as lubricant. Flow properties of granules like bulk density, tapped density, Carr index, Hausner’s ratio, angle of repose as well as physical parameters of the compressed tablets including hardness, friability, thickness and disintegration time were determined and found to be satisfactory. The FTIR spectroscopic analysis showed that the formulation containing plant gum is compatible with the drug and other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus domestica/pharmaceutics; Plant gum/binder property; Plant binder; Tablets/formulations; Sodium diclofenac/tablets/analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100020

Development and evaluation of a chronotherapeutic drug delivery system of torsemide
Provedor de dados: 60
Autores: Sunil,Songa Ambedkar; Rao,Nali Sreenivasa; Srikanth,Meka Venkata; Uhumwangho,Michael Uwumagbe; Kumar,Kommana Srinivas Phani; Murthy,Kolaplli Venkata Ramana.
The objective of this study was to prepare and evaluate chronotherapeutic drug delivery systems (ChrDDs) of torsemide. Compression coated tablets (CCT) containing torsemide in the core tablet were prepared by the compression coating technique with different grades of polyethylene oxide (PEO WSR 301 &amp;amp; 1105). The optimized formulations were characterised for tabletting parameters and drug polymer interaction by Fourier-Transform Infrared Spectroscopy (FTIR).The hardness of all the CCT using PEO WSR 301 &amp;amp; PEO WSR 1105 were in the range 6-8 kg/cm² &amp;amp; 5.5 to 7 kg/cm² respectively. Their friability values were &lt;0.3%. All the CCT showed a clear lag time but finalized as per the predetermined lag time. As the amount of PEO was increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Torsemide; Chronotherapeutic drug/delivery systems; Polyethylene Oxides; Compression coated tablets; Predetermined lag time.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300017

XVIII Pharmaceutical Congress of Sao Paulo: Contributions to the improvement of Pharmacy in Brazil
Provedor de dados: 60
Autores: Hirata,Rosario Dominguez Crespo.
Tipo: Info:eu-repo/semantics/other
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300001

Formulation and evaluation of buccoadhesive quetiapine fumarate tablets
Provedor de dados: 60
Autores: Potu,Appa Rao; Pujari,Naresh; Burra,Shashidher; Veerareddy,Prabhakar Reddy.
The aim of present study was to develop and evaluate buccoadhesive Quetiapine Fumarate (QF) tablets, which is extensively metabolised by liver. Buccoadhesive tablets of QF were prepared using HPMC K4M, HPMC K15M and combination of carbopol and HPC as mucoadhesive polymers by direct compression method. Sodium deoxycholate was added to formulation to improve the permeation of drug. The formulations were tested for bioadhesion strength, ex vivo residence time, swelling time and in vitro dissolution studies and ex vivo permeation studies. Optimized formulation (F3) showed 92% in vitro release in 8 h and 67% permeation of drug through porcine buccal mucosa and followed fickian release mechanism with zero order kinetics. FTIR studies of optimized formulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quetiapine Fumarate; Bioadhesion; In vivo mucoadhesive behaviour.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200017

Sustained virological response to treatment of chronic hepatitis C with peginterferon alfa and ribavirin
Provedor de dados: 60
Autores: Rosa,Junior André da; Blatt,Carine Raquel; Peres,Kaite Cristiane; Storb,Bernd Heinrich; Silva,Rochele; Farias,Mareni Rocha.
This study aimed to evaluate the rate of sustained virological response (SVR) and the clinical and treatment characteristics of patients with chronic hepatitis C (CHC). A retrospective uncontrolled cohort study was conducted among patients who received treatment for CHC between 2005 and 2008 attended at the Center for the Application and Monitoring of Injectable Medications, in Florianopolis, SC, Brazil. The inclusion criteria were: patients over 18 years of age, with a confirmed diagnosis of chronic hepatitis C according to Brazilian guidelines, treated with PEG-IFN alfa-2a or 2b associated with RBV. A total of 188 patients were included in the study: 70% men, 59% genotype 1, 27% coinfected with HIV, 31% with cirrhosis. The SVR rate, calculated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Hepatitis C Chronic; Effectiveness; Interferon alfa-2a; Interferon alfa-2b.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200002

Changes in weight and body fat after use of tetracycline and  Lactobacillus gasseri in rats
Provedor de dados: 60
Autores: Marciano,Jorge José; Fiol,Fernando de Sá Del; Ferreira,Alessandra Cristina Marciano Tardelli; Marques,Maria Cláudia; Santana,Luciane Lopes.
ABSTRACT Recent studies have shown a role of intestinal microbiota in obesity. The consumption of antibiotics in the last 70 years has led to changes in intestinal microbiota, which has led to weight gain and body fat accumulation. To evaluate the possibility of weight gain induced by antibiotics and the possible protective effect of probiotics, we divided 45 animals (Rattus norvegicus) into groups and administered the following treatments over two weeks: tetracycline, tetracycline + Lactobacillus gasseri, and NaCl. The animals were weighed over the course of 8 weeks, and at the end of the treatment period, they were measured and subjected to bioelectrical impedance analysis. The results show that the group receiving tetracycline alone had a higher body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobials/evaluation; Obesity/tests; Gut microbiota/tests; Tetracyclines.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100619

Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients
Provedor de dados: 60
Autores: Mark,Raymond; Huri,Hasniza Zaman; Razack,Azad Hassan Abdul.
The incidence of erectile dysfunction (ED) is rising worldwide and its prevalence is one of the main health concerns that affect overall men well-being in Malaysia. The cluster of demographic, clinical and lifestyle factors may have contributed to the severity of ED and changes in biomarkers level; nevertheless these have not been studied extensively. This cross sectional study involved a total of 276 patients with 138 was diagnosed with ED. The demographic, clinical, lifestyle factors and severity of ED were assessed using a set of questionnaire and the International Index of Erectile Function (IIEF-5). Meanwhile, Total Testosterone (TT) and Asymmetric dimethylarginine (ADMA) levels were determined using the enzyme-linked immunosorbant assay (ELISA)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erectile dysfunction/biomarker/clinical; Demography; Lifestyle; Men’s Health; Malaysia.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300615

Safety and efficacy of ketamine xylazine along with atropine anesthesia in BALB/c mice
Provedor de dados: 60
Autores: Jamal,Muhammad Ameen; Ahmed,Arslan Mahmood; Tahir,Muhammad; Ashraf,Muhammad; Sattar,Abdul; Ghafoor,Aamir; Munir,Shahzad; Ahmed,Irfan; Hussain,Mubashir; Riaz,Amjad.
Anesthetics are an indispensable prerequisite for surgical intervention and pharmacological animal studies. The objective of present study was to optimize the dose of ketamine (K) and xylazine (X) along with atropine sulfate (A) in order to achieve surgical tolerance in BALB/c mice. Several doses of ketamine (100, 150, 200 mg/kg) and xylazine (10, 15, 20 mg/kg) were mixed and combination of nine doses (K/X: 100/10, 100/15, 100/20, 150/10, 150/15, 150/20, 200/10,200/15,200/20) were evaluated (n=9 per combination). A constant dose of atropine (0.05 mg/kg) was also used to counter side effect. Time-related parameters were evaluated on the basis of reflexes. KX at dose 200/20 mg/kg produced surgical tolerance in all nine mice with duration 55.00±6.87 minutes....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ketamine hydrochloride; Xylazine hydrochloride; Atropine sulfate; Surgical tolerance; BALB/c mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100556

Development and validation of an HPLC method for stability evaluation of nystatin
Provedor de dados: 60
Autores: Cione,Ana Paola Prata; Liberale,Márcio José; Silva,Paulo Marcos da.
A liquid chromatography method for evaluating the stability of Nystatin (Nys) in an ointment was developed and validated, since the traditional pharmacopeial microbiological methods are unable to indicate stability. The stress experiments showed that Nys was found to significantly degrade in alkaline and acidic conditions and also under oxidative stress. Lower levels of degradation were detected under heat and with the sample exposed to Xenon light. Resolutions higher than 2 for Nys and degradation products (DP) chromatographic peaks were achieved by using an Inerstil ODS-3 column, isocratic elution with methanol:water and UV detection at 305 nm. The system was found to be linear over a range of 102 to 310 IU mL-1 and proved precise, since the RSD(%) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nystatin/stability analysis; Forced degradation/stress; High performance liquid chromatography/qualitative analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200018

Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549)
Provedor de dados: 60
Autores: Robinson,Jayachandran Philip; Suriya,Kumaresan; Subbaiya,Ramasamy; Ponmurugan,Ponnusamy.
ABSTRACT Human have been constantly using plants and plant products to overcome many diseases. The antioxidant property of the plant sources is studied to obtain an efficacious drug against cancer. The objectives of the present study is to evaluate the antioxidant and cytotoxic activity of the Tecoma stans extracts against lung cancer cell line in comparison with vincristine drug. The antioxidant activity was studied using the standard DPPH assay and the cytotoxic activity using MTT assay. DPPH assay results show that methanolic extract of T. stans in higher concentration show better antioxidant potential than the standard L-ascorbic acid. They exhibited strong antioxidant potential at 20 µg/mL concentration. The absorbance at 517 nm showed that in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tecoma stans/extract/cytotoxicity; Tecoma stans/extract/antioxidant activity; Free radical scavenging; Plant extracts; Lung cancer cell line/study/drugs.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300605

Comparison of microbiological assay and HPLC-UV for determination of fluconazole in capsules
Provedor de dados: 60
Autores: Queiroz,Kelly Marques; Silva,Maria-Luiza Martins; Prado,Nathália Duque; Lima,Paulo Marcelo Andrade; Diniz,Rosiane Dias Lopes; César,Isabela Costa; Pianetti,Gerson Antônio; Santos,Daniel Assis.
The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r²=0.9995), precision (R.S.D. = 4.0% for intra-day and 4.5% for inter-day precision) and accuracy (mean recovery = 102.9%). High performance liquid chromatography was chosen as a comparison method for the fluconazole determination. The contents of fluconazole determined by both methods, for four capsule samples, showed a strong correlation, confirmed by Pearson's correlation coefficient value (r = 0.9884). The bioassay is a suitable method for both research and pharmaceutical industry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluconazole/quantitative determination; Microbiological assay/agar diffusion; High performance liquid chromatography.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400012

Antimicrobial susceptibility patterns among bacteria isolated from intensive care units of the largest teaching hospital at the northwest of Iran
Provedor de dados: 60
Autores: Hamishehkar,Hadi; Shadmehr,Pegah; Mahmoodpoor,Ata; Mashayekhi,Simin Ozar; Entezari-Maleki,Taher.
ABSTRACT This study was conducted to determine the antimicrobial susceptibility patterns among common pathogens in the intensive care units (ICUs) of a university hospital in northwestern Iran. A retrospective study was done on laboratory records of patients with nosocomial infection who were admitted to five ICUs of Imam Reza Hospital during a 21-month period from March 2010 to January, 2012. A total number of 556 isolates from 328 patients were evaluated. The most common sites of infections included respiratory (51.7%), urinary (24.8%), and blood (10.4%). The most frequently isolated microorganisms were Enterobacter aerogenes (50.6%) followed by Escherichia coli (16.7%) and Pseudomonas aeruginosa (7.5%). Staphylococcus aureus was the most frequent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intensive care units/anti-infective agents/cross infection; Hospital University/Iran.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300403

Effect of herb-drug interactions of Bacopa monnieri Linn. (Brahmi) formulation on the pharmacokinetics of amitriptyline in rats
Provedor de dados: 60
Autores: Khurshid,Fowad; Govindasamy,Jeyabalan; Khalilullah,Habibullah; Nomani,Mohammed Shivli; Shahid,Mudassar; Ain,Md Ruhal; Alsultan,Mohammad Sultan.
ABSTRACT Interactions between herbs and drugs may increase or decrease the pharmacological or toxicological effects of either component. Experimental data on the pharmacokinetic interactions between herbal products and drugs are limited. This study attempted to investigate the effect of Bacopa monnieri Linn. (Brahmi) formulation on the pharmacokinetics of amitriptyline in rats. In this study, rats were randomly divided into two groups (n = 6 each) which were served as a control (amitriptyline alone) and treatment group (amitriptyline with B. monnieri), respectively. Rats in the treatment group received B. monnieri (31 mg/kg/day) whereas the control group received normal saline by oral gavage for seven days before a single intragastric administration of 25...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbal drug interaction; Bacopa monnieri/effects; Amitriptyline/pharmacokinetics; CYP450; Bioavailability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400610

Effects of granule particle size and lubricant concentration on tablet hardness containing large concentration of polymers
Provedor de dados: 60
Autores: Rajani,Chauhan; Kumar,Dewangan Devendra; Jaya,Dwivedi; Kumar,Jha Arvind.
ABSTRACT The objective of this research work is to demonstrate the impact of granule size and lubricant concentration on the hardness of tablets in formulations containing higher concentration of polymers and to resolve the hardness issue during compression process. The work involves optimization of a milling process for size reduction of granules and blending process to achieve tablets of good hardness on compression. To optimize the granule size, different sized co-mill screens were used. The different concentration of lubricant were studied on different sized granules to check the effect on hardness of tablets and to obtained the desired hardness of tablets. Compression of lubricated blend in various concentration was performed using the gravity feeder...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tablet hardness/effects; Granule size; Lubricant concentration; Polymer.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300617

Study of the variables which influence the impregnation of globules, compressed tablets and tablet triturates used in homeopathy
Provedor de dados: 60
Autores: Souza,Fernanda Santos de; Pirolli,Mayara Marin; Diehl,Eliana Elisabeth; Sonaglio,Diva.
Globules, compressed tablets and tablet triturates are solid dosage forms used in homeopathy. Divergences can be noted between the preparation techniques described in official compendiums as well as those applied in homeopathic pharmacies. The difficulty associated with standardization of the impregnation of these dosage forms occurs due to the lack of detail provided for the techniques in the literature, leaving it up to each pharmacy to decide on the exact method of preparation. The objective was to optimize the impregnation technique, through investigating the variables that influence the impregnation of globules, compressed tablets and tablet triturates, applying the statistical tool of factorial design. The independent variables were the dosage form,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homeopathic pharmacotechniques; Homeopathic vehicles; Homeopathy/impregnation methods; Homeopathy/globules; Homeopathy/tablet triturates; Homeopathy/compressed tablets.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300021

Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves
Provedor de dados: 60
Autores: Silva,Joliane Carvalho; Azevedo,Pedro Simão da Silva; Souza,Fabiana de Moura; Aragão,Kalynca Kayla Viana; Sabino,Carla Kelly Barroso; Carvalho,Gabriela Dantas; Morais,Ilmara Cecília Pinheiro; Mendes,Marcelo Bezerra; Chaves,Mariana Helena; Oliveira,Aldeídia Pereira de.
The study evaluated the vasorelaxant effect induced by the ethanolic extract of the leaves of Zanthoxylum rhoifolium Lam (EEtOH-Zr/leaves). Wistar rats were treated with the leaf extract containing a single dose of 2,000 mg / kg, v.o. After 14 days, the animals were anesthetized for blood collection and subsequent analysis of the biochemical parameters; they were then euthanized (sodium pentobarbital-100 mg/kg, i.p.) for the removal and morphological analysis of the heart, lung, liver and kidney. The vasorelaxation activity the and vascular reactivity of EEtOH-Zr/leaves were evaluated on artery mesenteric rings isolated from rats. The extract showed no signs of toxicity and no significant difference in the values of the biochemical parameters between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenteric arteries/ drug effects; Vasorelaxant; Zanthoxylum rhoifolium/toxicity; Rats Wistar; Blood Vessels/ drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100533

Optimization and validation of an RP-HPLC method for the estimation of 6-mercaptopurine in bulk and pharmaceutical formulations
Provedor de dados: 60
Autores: Somasekhar,Vanita.
A reverse phase HPLC method is described for the determination of 6-mercaptopurine in bulk and tablets. Chromatography was carried on a C18 column using a mixture of acetonitrile and 0.05 mol/L sodium acetate buffer (10:90 v/v) as the mobile phase at a flow rate of 1 mL/min-1 with detection at 324 nm. The retention time of the drug was 3.25 min. The detector response was linear in the concentration of 0.01-5 μg/mL. The limit of detection and limit of quantification were 17 and 52 ng/mL respectively. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of mercaptopurine in bulk and tablets.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 6-Mercaptopurine/determination; High perfomance liquid chromatography/reverse phase/quantitative analysis; Pharmaceutical formulations/quality control.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400793

Evaluation of cytotoxicity and mutagenicity of the benzodiazepine flunitrazepam in vitro and in vivo
Provedor de dados: 60
Autores: Almeida,Igor Vivian de; Domingues,Giovana; Soares,Lilian Capelari; Düsman,Elisângela; Vicentini,Veronica Elisa Pimenta.
Flunitrazepam (FNZ) is a sedative benzodiazepine prescribed for the short-term treatment of insomnia. However, there are concerns regarding possible carcinogenic or genotoxic effects of this medicine. Thus, the aim of this study was to evaluate the cytotoxic, clastogenic and aneugenic effects of FNZ in hepatoma cells from Rattus norvegicus (HTC) in vitro and in bone marrow cells of Wistar rats in vivo. These effects were examined in vitro following treatment with 0.2, 1.0, 5.0 or 10 μg/mL FNZ using a micronucleus test with a cytokinesis block or in vivo using a chromosomal aberration test following treatment with 7, 15 or 30 μg/mL/kg body weight. The results showed that the benzodiazepine concentrations tested were not cytotoxic, aneugenic or clastogenic....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flunitrazepam/cytotoxic effects; Flunitrazepam/clastogenic effects; Flunitrazepam/aneugenic effects; Sedative benzodiazepine/adverse effects/in vitro and in vivo study; Chromosomal abnormalities/medicines effects.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200251

Essential oil of the leaves of Eugenia sulcata preserve myocardial contractility and does not present immunotoxicity
Provedor de dados: 60
Autores: Santos,Karolina Torres; Silva,Fabricio Ocampo da Luz e; Schneider,Leandro Alves; Santos,Carlos Eduardo Rangel dos; Silva,Sue Elle Berro da; Fernandes,Maquelen Blanco; Rocha,Leandro; Oliveira,Luís Flávio Souza de; Machado,Michel Mansur; Moreira,Cleci Menezes.
The essential oil of the leaves of Eugenia sulcata, in the Myrtaceae family, has a demonstrated antihypertensive effect, but its effects on heart muscle and its toxicity have not yet been elucidated. Little chemical or biological data are available for E. sulcata, whether emphasizing the beneficial effects or the pharmacological security of this species. This study aims to evaluate myocardial contractility and to analyze angiotensin converting enzyme (ACE) and myosin ATPase activities associated with use of this essential oil. In addition, we evaluated the immunotoxicity of E. sulcata essential oil. Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) were treated daily for 30 days (10 mg/kg of oil) to evaluate the isometric force of the papillary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrtaceae/adverse effects; Angiotensin-Converting Enzyme Inhibitors/immunology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100532

The main potentialities of resveratrol for drug delivery systems
Provedor de dados: 60
Autores: Ruivo,Joana; Francisco,Cátia; Oliveira,Rita; Figueiras,Ana.
Resveratrol (RSV), a natural polyphenol found in grapes, was found to be effective in the prevention and therapy of several diseases, however, it does have unfavourable physicochemical properties. In this context, an increasing number of studies have aimed at developing novel therapeutic systems for its delivery to overcome these disadvantages. This review focuses on the mechanisms of action and therapeutic applications. Finally, it also describes some tested formulations for RSV administration, controlled release and targeting, developed with the purpose of increasing RSV bioavailability.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutraceuticals; Natural polyphenols; Resveratrol/mechanisms of action; Resveratrol/therapeutic applications; Resveratrol/delivery systems.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300499

Formulation of gastroresistant tablets containing sodium alendronate-loaded blend microparticles
Provedor de dados: 60
Autores: Ferreira,Luana Mota; Velasquez,Aline de Arce; Schaffazick,Scheila Rezende; Cruz,Letícia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microparticulated tablets/release profile; Sodium alendronate/oral administration; Sodium alendronate/controlled release/in vitro study.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200323

Application of capillary electrophoresis to the simultaneous determination and stability study of four extensively used penicillin derivatives
Provedor de dados: 60
Autores: Simon,Brigitta; Hancu,Gabriel; Gyéresi,Árpád.
The applicability of capillary electrophoresis for the analysis of four extensively used penicillin derivatives (benzylpenicillin, ampicillin, amoxicillin, oxacilllin) has been studied. Because of structural similarities, the electrophoretic behavior of these derivatives is very similar; consequently an efficient separation using the conventional capillary zone electrophoresis is hard to be achieved. Their simultaneous separation was solved by using micellar electrokinetic capillary chromatography, the separation being based on the differential partition of the analytes between the micellar and aqueous phase. Using a buffer solution containing 25 mM sodium tetraborate and 100 mM sodium dodecyl sulfate as surfactant, at a pH of 9.3, applying a voltage of +...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillin/derivatives/stability study; Capillary electrophoresis/drugs analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300521

Glycodendritic structures: tools to interact with DC-SIGN
Provedor de dados: 60
Autores: Reina,Jose J.; Rojo,Javier.
The key role of carbohydrates in many biological events has attracted the interest of the scientific community. This fact has demanded the access to new tools necessary to understand this role and the interaction of carbohydrates with their corresponding receptors, lectins. Glycodendrimers and glycodendritic structures in general, have demonstrated to be very efficient and interesting tools to intervene in those processes where carbohydrates participate. In this review, we discuss the different glycodendritic structures that have been used to interfere with DC-SIGN, a very attractive lectin involved in infection processes and in the regulation of the immune response.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DC-SIGN; Ebola; Glycodendrimers; Glycofullerenes; HIV; Mannose.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700009

Acetylcholinesterase inhibition, antioxidant and identification of some chemical constituents of Phyllanthus atropurpureus cultivated in Egypt
Provedor de dados: 60
Autores: Abdallah,Walid Elsayed; Elsayed,Wael Mohamady; Hassan,Rasmia Ali; Nazif,Naglaa Mohammed; Ali,Mohammed Ibrahim; Abdelshafeek,Khaled Abdelhady.
Investigation of the lipid constituents of the aerial parts of Phyllanthus atropurpureus resulted in isolation and identification of the fatty acid mixture which consists of eight acids with linolenic acid as major and the unsaponifiable fraction that contain a series of hydrocabons, sterols, in addition to one triterpene (α-amyrin). The acetone insoluble fraction was found to contain two fatty alcohols and three n-hydrocabons in which the n-eicosane is the most abundant (44.16%). The flavonoidal constituents were isolated from ethyl acetate and butanol fractions which were identified as: luteolin-7-O-glucoside, kaempferol 3-O-(p-coumaroylglucoside), kaempferitrin, luteolin and kaempferol. Evaluation of different extracts as acetylcholinesterase inhibitors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phyllanthus atropurpureus; Euphorbiaceae; Flavonoids; Acetylcholineesterase inhibitors; Antioxidant.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100575

Ortho-nitrobenzyl derivatives as potential anti-schistosomal agents
Provedor de dados: 60
Autores: Lopes,Marcela Silva; Suzuki,Brian Michio; Pereira,Glaécia Aparecida do Nascimento; Probst,Alexandra Christina; Ferreira,Rafaela Salgado; Oliveira,Júlia Teixeira de; Tecchio,Kimberly Brito; Santos,Fabio Vieira dos; Caffrey,Conor Robert; Oliveira,Renata Barbosa de.
ABSTRACT In the search for new anti-schistosomal agents, a series of fifteen ortho-nitrobenzyl derivatives was assayed in vitro against both the schistosomulum (somule) and adult forms of Schistosoma mansoni. Compounds 8 and 12 showed significant activity against somules at low micromolar concentrations, but none was active against adults. The SAR demonstrated that the compounds most active against the parasite were mutagenic to the human cell line RKO-AS45-1 only at concentrations 10- to 40-fold higher than the worm-killing dose. Given their electrophilicity, compounds were also screened as inhibitors of the S. mansoni cysteine protease (cathepsin B1) in vitro. Amides 5 and 15 exhibited a modest inhibition activity with values of 55.7 and 50.6 % at 100...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitro-aromatic; Schistosoma mansoni/ anti-schistosomal activity; Cathepsin B1; Mutagenicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200613

Use of unlicensed and off-label drugs in neonates in a Brazilian university hospital
Provedor de dados: 60
Autores: Gonçalves,Adriana Cristina de Souza; Reis,Adriano Max Moreira; Marçal,Ana Carolina Gusmão; Bouzada,Maria Cândida Ferrarez.
ABSTRACT This study was designed to investigate the use of off-label and unlicensed drugs in a Neonatal Care Unit (NCU) and to compare the frequency of use of off-label drugs according to the drug regulatory agencies in Brazil (Agência Nacional de Vigilância Sanitária-ANVISA) and the United States Food and Drug Administration (FDA). A prospective observational study was carried out in the NCU. Prescriptions were classified as off-label and unlicensed using both ANVISA and FDA criteria. A total of 157 newborns and 1187 prescriptions were analyzed. The most prescribed drug was fentanyl (9.3%), followed by multivitamin (8.4%) and gentamicin (7.9%). According to ANVISA criteria, there were 665 (56.0%) off-label prescriptions and 86 (7.2%) unlicensed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/use/University Hospital/Brazil; Drug/use/off-label/unlicensed; Drug therapy; Neonatology; University Hospital/Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300622

Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis - an in vitro assay
Provedor de dados: 60
Autores: Ebrahimzadeh,Mohammad Ali; Khalili,Masoumeh; Dehpour,Abbas Ali.
ABSTRACT The aim of this study was to evaluate the antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis. The extracts were assayed for total phenol and flavonoid content, DPPH free radical scavenging capacity, nitric oxide activity, iron chelation activity, and reducing power activity. Total phenol and flavonoid content were found to be high in both algae. Ethyl acetate extracts of both algae were found to exhibit significant antioxidant activity. Ethyl acetate extract of N. oculata exhibited a good capacity for iron chelation, nitrate oxide, and scavenging DPPH free radicals (72.95±2.30, 73.73±1.76, and 39.03±0.97% inhibition at 400 µg mL-1 respectively).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine algae/antioxidant activity; Nannochloropsis Oculata/total phenol/total flavonoid/ Gracilaria Gracilis/total phenol/total flavonoid.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100614

Effect on hyperglycemia and pancreas cells of chaya aqueous extracts from two different regions in streptozotocin-induced diabetes rats
Provedor de dados: 60
Autores: Guzmán,Eduardo Lozano; González,José Cruz Calderón; Flores,Maribel Cervantes; Carrillo,Ada Sandoval; Pescador,María Guadalupe Nieto; Cruz,Francisco Javier Moreno.
Cnidoscolus chayamansa is a native plant of the Mayan region, which is also cultivated in other places like northern Mexico, Tunisia and India. Many properties are attributed to Mayan Chaya, such as aid in the control of glycemia in diabetics. Thus this study aimed to evaluate the hypoglycemic effects of chaya aqueous extracts in a model of streptozotisin-induced diabetic Wistar rats. Chaya aqueous extracts were collected from plants cultivated in Quinta Roo (Mayan region) and Durango (northern Mexico), and in this study we compare their effect with metformin (as a control). Additionally, we compared the extracts mass profiles from both regions by high-resolution liquid chromatography coupled to a triple quadrupole tandem mass detector (HPLC-MS/MS QQQ)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Chaya; Langerhans islets; Hypoglycemia; HPLC.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100619

Pharmaceutical sciences scenario in CNPq research fellowship
Provedor de dados: 60
Autores: Guterres,Silvia Stanisçuaski; Lopes,João Luis Callegari; Barreiro,Eliezer J.; Mello,João Carlos Palazzo de.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400001

Caffeine Supplementation and muscle damage in soccer players
Provedor de dados: 60
Autores: Machado,Marco; Breder,Anselmo Carvalho; Ximenes,Marcio Carvalho; Simões,Jarbas Rodrigues; Vigo,José Fábio Florentino.
The aim of this work was to evaluate the effect of caffeine supplementation and intermittent exercise on the muscle injury markers in soccer players. 15 male professional soccer players completed a placebo controlled double blind test protocol. 45 minutes before exercise, participants ingested 5.5 mg.kg-1 body mass of caffeine (EXP, n=8) or placebo (CONT, n=7). The exercise was 12 sets of 10 sprints (20 m each) with 10 sec recovery time between sprints and 2 min between sets. Blood samples were collected before (PRE) and 48h after exercise (POST). Serum activity of CK, LDH, AST, and ALT were quantified. Serum enzyme activity was enhanced by exercise in both groups, without a synergistic effect of caffeine. The findings suggest muscle injury markers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle injury/markers; Transaminases; Physical exercise; Caffeine/supplementation; Soccer; Creatine kinase; Lactate dehydrogenase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200010

Development of agar diffusion method for dosage of gramicidin
Provedor de dados: 60
Autores: Solano,Ana Gabriela Reis; Pereira,Larissa de Melo Campos Sousa; Leonel,Míriam de Fátima Vianna; Nunan,Elzíria de Aguiar.
Gramicidin, an antimicrobial peptide active against Gram positive bacteria, is commonly used in pharmaceutical preparations for topical use. Considering that only the turbidimetric method has been described in the literature, the present study sought to develop and validate an agar diffusion method for the dosage of gramicidin. The method was developed and validated using the Kocuria rhizophila ATCC 9341 as a test microorganism. Two designs were used: a 3x3 parallel-line model, and a 5x1 standard curve. The validation demonstrated that the method follows the linear model (r²= 0.994), presenting a significant regression between the zone diameter of growth inhibition and the logarithm of the concentration within the range of 5 to 25.3 µg/mL. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gramicidin/microbiological assay; Microbiological assay/method validation; Agar diffusion; Cylinder-plate.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300014

Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity
Provedor de dados: 60
Autores: Garcia,Israel José Pereira; Cézar,Jessica Silva; Lemos,Bruno Silva; Silva,Lilian Nayra; Ribeiro,Rosy Iara Maciel de Azambuja; Santana,Camilla Camerino; Grillo,Luciano Aparecido Meireles; Pinto,Flavia Carmo Horta; Buzelle,Samyra Lopes; Cortes,Vanessa Faria; Santos,Hérica de Lima; Santos,Maria Emilia Soares Martins dos; Barbosa,Leandro Augusto.
ABSTRACT It is widely known that high fat diet (HFD) can contribute to the advent of health problems. Recent studies have indicated that obesity imposes a hemodynamic overload to the kidneys. In order to further investigate such injuries, two groups of six Swiss mice each were fed with a controlled AIN93G diet or a high fat (AIN93G modified) diet for eight weeks. Blood samples were collected to determine the hormonal, lipid profile, glucose, urea, and creatinine levels. Histopathological and immunohistochemical analysis were carried out to analysis the kidney damage. Fractions of renal membranes were prepared to assess the Na,K-ATPase activity, lipid peroxidation, total cholesterol, and phospholipid content. The results indicated that the blood lipid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High fat diet/Effects Na; K-ATPase Lipid peroxidation Kidney Cell membrane.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100607

Ion-pairing liquid chromatographic method determination of ketoconazole and stress degradation in pharmaceutical formulations with fluorescence detection
Provedor de dados: 60
Autores: Thongchai,Wisanu.
Ion-pairing liquid chromatographic method was validated for determination of ketoconazole in shampoo and cream samples as per ICH guidelines. The chromatographic conditions were carried out in the isocratic mode using a mixture of methanol and 8 mM sodium dodecyl sulfate (pH 5.5) in a ratio of 45:55 v/v %, as mobile phase. The flow rate was set at 1.0 mL min-1. Chromolith RP-18e (100×4.6 mm) was used as the analytical column with a fluorescence detection at an excitation wavelength of 260 nm and an emission wavelength of 375 nm. The average percentage recovery of shampoo A, shampoo B, shampoo C, cream A and cream B were 99.88, 97.06, 99.58, 96.77 and 97.26, respectively. The limit of detection was 0.12 mg L-1. The drug decomposition under acid degradation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ion-pairing liquid chromatography; Ketoconazole; Shampoo; Cream; Fluorescence detection.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100538

Correlation between rheological properties and capillary fixation for modelling creams
Provedor de dados: 60
Autores: Demoliner,Lara Brogni; Agnol,Lucas Dall; Zanotti,Suzana Bittencourt; Dias,Fernanda Trindade Gonzalez; Angeli,Valeria Weiss; Bianchi,Otavio.
The Brazilian male beauty market occupies the second place in world consumption of cosmetics. Among the numerous products consumed by such audience is the capillary fixation mask, which is mainly composed by fixatives. These additives act on stabilizing the cosmetic emulsion, protecting the hair against moisture and also increasing the intensity of hair fixation. In this work, three formulations for modelling creams were prepared by different concentrations of the fixatives StylezeTM W20 and PVP K90 and their properties characterized by physicochemical, rheological and sensory analysis. The capillary masks produced were stable oil-in-water (O/W) emulsions with uniform droplets of 2.05-2.82 μm sizes and pseudoplastic thixotropic behavior (0.19 &lt; n &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheology; Microscopy; N-vinyl-2-pyrrolidone; Polyquaternium-55; Modelling cream/physicochemical properties; Cosmetics.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300621

Implementation of a penicillin allergy skin test
Provedor de dados: 60
Autores: Nagao-Dias,Aparecida Tiemi; Pereira,Ana Carla; Silva,Michelly Freitas e; Néri,Eugenie Desiree Rabelo; Accioly,José Wilson; Lima,Janete Elisa Soares.
The penicillin allergy skin testing is the only accurate and reliable test for penicillin hypersensitivity mediated by IgE. It is useful for identifying patients with doubtful history of allergy. Positive test for major and minor determinants presents a positive predictive value of 50% and negative predictive value of 99%. In Brazil, the Ministry of Health suggests a protocol for in house made reagents, since they are not commercially available. As the referred protocol does not mention some important details about the test procedures, we propose in the present work to implement them, critically evaluating each step in order to allow the protocol establishment at any health service, with quality and safety.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicilin/allergy; Penicilin/cutaneous test; Skin test/penicilin allergy; Betalactamic/hipersensitivity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300023

In vitro UV absorption properties and radical scavenging capacity of Morella parvifolia (Benth.) Parra-Os. extracts
Provedor de dados: 60
Autores: Puertas-Mejía,Miguel Angel; Gutierrez-Villegas,María Isabel; Mejía-Giraldo,Juan Camilo; Winkler,Robert; Rojano,Benjamín.
The goal of this research was to identify major compounds of the aerial parts of M. parvifolia (Benth.) Parra-Os., that could enhance its possible application as additive in dermocosmetic products, as well as evaluate the antioxidant properties. The extracts agreed with the broad-spectrum UVB/UVA absorption detected and could act as broad-spectrum sunscreens, covering the UVA and UVB range. Methanolic extracts showed an important antiradical capacity (0.46 and 0.47 g/µmol DPPH), TPC (37.58 and 51.41mg GAE/g DS) and TAC (1.12 and 3.31 mg C3GE/g DS) in fruits and leaves, respectively. M. parvifolia could be considered as a prospective source of natural UV-radiation absorbers with antioxidant capacity. Although the results have clearly demonstrated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morella parvifolia; Potential skin barrier; UV absorption capacity; Pharmaceutical additive.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300614

Targeting of temozolomide using magnetic nanobeads: an in vitro study
Provedor de dados: 60
Autores: Gürten,Berna; Yenigül,Elçin; Sezer,Ali Demir; Altan,Cem; Malta,Seyda.
Temozolomide, a chemotherapeutic drug that is often administered for the treatment of brain cancer has severe side effects and a poor aqueous solubility. In order to decrease the detrimental effect of the drug over healthy cells, a novel drug delivery vehicle was developed where the therapeutic drug was encapsulated within the hydrophobic cavities of b-CD modified magnetite nanoparticles, which are embedded in chitosan nanobeads prepared by salt addition. In-vitro studies have shown that the magnetic properties of the novel delivery vehicle are adequate for targeted drug delivery applications under an external magnetic field. Additionally, an increase in the amount of chitosan was shown to exhibit a strong shielding effect over the magnetic properties of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temozolomide; Magnetite; Cyclodextrin; Chitosan; Targeting; Anticancer.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100574

Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant Staphylococcus aureus clinical isolates
Provedor de dados: 60
Autores: Cavalcanti,Isabella Macário Ferro; Menezes,Talita Gomes Calaça; Campos,Luís André de Almeida; Ferraz,Milena Sales; Maciel,Maria Amélia Vieira; Caetano,Maria Nelly Psiotano; Santos-Magalhães,Nereide Stela.
ABSTRACT The treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of VAN with usnic acid and β-lapachone encapsulated into liposomes as a novel therapeutic option for infections caused by MRSA. Liposomes containing β-lap (β-lap-lipo) or usnic acid (UA-lipo) were prepared by the thin lipid film hydration method followed by sonication. Antimicrobial activity against MRSA clinical isolates was investigated by the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The interaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-lapachone; Usnic acid; Liposomes; Methicillin-resistant Staphylococcus aureus (MRSA); Synergism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200627

Anticonvulsant activity of Bacopa monniera in rodents
Provedor de dados: 60
Autores: Kaushik,Darpan; Tripathi,Ashish; Tripathi,Rashmi; Ganachari,Madiwalayya; Khan,Suroor Ahmad.
Bacopa monnieri (L), belonging to the Scrophulariaceae family and commonly known as Brahmi, is well known in India for its CNS activity but its neuropharmacological effect has not yet been explored. In the present study, the antiepileptic effects of the plant were investigated. The ethanolic extract of Bacopa monniera was tested for anticonvulsant activity in albino rats, using different convulsive models. The ethanolic extract of leaves produced significant anticonvulsant activity for all the different models studied. The present study shows a probable mechanism of action similar to that of benzodiazepines (GABA agonist). Thus, these results emphasize the need to diversify by using alternative therapeutic approaches pertaining to herbal medicine, where a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacopa monniera/pharmacognosy; Anticonvulsant; MES test; PTZ model; Hypoxia model; Strychnine model.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400006

A novel effect of geraniin on OPG/RANKL signaling in osteoblasts
Provedor de dados: 60
Autores: Zhang,Xiaochao; Yang,Renhua; Long,Jiang; He,Bo; Zhang,Yue; Fu,Ting; Shen,Zhiqiang; Chen,Peng.
In this study, the effects of geraniin on osteoprotegerin/receptor activator of nuclear factor-κB ligand(OPG/RANKL) in regulating the proliferation of osteoblasts and suppression of osteoclast-like cells (OLC) in OLC-osteoblast co-cultured system in vitro were investigated. Osteoblasts were cultured and identified with alkaline phosphatase (ALP), gomori stain, and mineralized nodule stain. OLCs were isolated from long bones of Sprague-Dawley (SD) rats and identified with tartrate-resistant acid phosphatase(TRAP) stain. Methyl thiazolyl tetrazolium assay was used to examine the proliferation of osteoblasts, and immunocytochemistry and in situ hybridization to analyze the expression OPG/RANKL in osteoblasts co-cultured with osteoclasts under the action of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geraniin/effect; Osteoblasts; Osteoprotegerin; Receptor activator of nuclear factor -κ B ligand.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300618

Pharmacovigilance in oncology: pattern of spontaneous notifications, incidence of adverse drug reactions and under-reporting
Provedor de dados: 60
Autores: Visacri,Marília Berlofa; Souza,Cinthia Madeira de; Pimentel,Rafaela; Barbosa,Cristina Rosa; Sato,Catarina Miyako Shibata; Granja,Silvia; Marialva,Mécia de; Lima,Carmen Silvia Passos; Mazzola,Priscila Gava; Moriel,Patrícia.
The high toxicity and narrow therapeutic window of antineoplastic agents makes pharmacovigilance studies essential in oncology. The objectives of the current study were to analyze the pattern of spontaneous notifications of adverse drug reactions (ADRs) in oncology patients and to analyze the incidence of ADRs reported by outpatients on antineoplastic treatment in a tertiary care teaching hospital. To compose the pattern of ADR, the notification forms of reactions in oncology patients in 2010 were reviewed, and the reactions were classified based on the drug involved, mechanism, causality, and severity. To evaluate the incidence of reactions, a questionnaire at the time of chemotherapy was included, and the severity was classified based on the Common...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacovigilance; Oncovigilance; Drugs/adverse reactions; Adverse drug reactions/under-reporting; Adverse drug reactions/Spontaneous notification; Clinical pharmacy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200411

Equilibrium solubility versus intrinsic dissolution: characterization of lamivudine, stavudine and zidovudine for BCS classification
Provedor de dados: 60
Autores: Dezani,André Bersani; Pereira,Thaisa Marinho; Caffaro,Arthur Massabki; Reis,Juliana Mazza; Serra,Cristina Helena dos Reis.
Solubility and dissolution rate of drugs are of major importance in pre-formulation studies of pharmaceutical dosage forms. The solubility improvement allows the drugs to be potential biowaiver candidates and may be a good way to develop more dose-efficient formulations. Solubility behaviour of lamivudine, stavudine and zidovudine in individual solvents (under pH range of 1.2 to 7.5) was studied by equilibrium solubility and intrinsic dissolution methods. In solubility study by equilibrium method (shake-flask technique), known amounts of drug were added in each media until to reach saturation and the mixture was subjected to agitation of 150 rpm for 72 hours at 37 ºC. In intrinsic dissolution test, known amount of each drug was compressed in the matrix of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/solubility; Drugs/dissolution rate; Intrinsic dissolution/method; Intrinsic dissolution/biopharmaceutical classification; Biopharmaceutical Classification System.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400026

Oxidative stress and histopathologic biomarkers of exposure to bisphenol-A in the freshwater fish, Ctenopharyngodon idella
Provedor de dados: 60
Autores: Faheem,Mehwish; Lone,Khalid Parvez.
ABSTRACT Bisphenol-A (BPA) belongs to the family of endocrine disrupting chemicals (EDCs) and it is used in the production of polycarbonate plastic and epoxy resins. The reproductive toxicity of BPA is well documented but it also exerts its toxic effects through multiple pathways especially by inducing a state of oxidative stress and causing damage to the vital organs. In the present study, histopathologic and oxidative damage caused by BPA in liver and kidneys of fresh water cyprinid, Ctenopharyngodon idella was evaluated. LC50 of BPA for Ctenopharyngodon idella was determined by probit regression analysis. Fish were exposed to a sublethal concentration of BPA i.e. 3.2 ppm (1/2 LC50) for 14 days. Histologic studies revealed that BPA caused degenerative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bisphenol-A/effects; Histopathology; Ctenopharyngodon idella/endocrine disrupting chemicals/Oxidative stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300624

Cytotoxic and genotoxic investigation on barbatimão [Stryphnodendron adstringens (Mart: ) Coville, 1910] extract
Provedor de dados: 60
Autores: Vilar,Juliana Brandstetter; D'Oliveira,Maria Inez Prudente; Santos,Suzana da Costa; Chen,Lee Chen.
Stryphnodendron adstringens (Mart.) Coville, 1910 is a small tree, distributed widely throughout the Cerrado region of Brazil and named "barbatimão" by the Tupi-Guarani tribes, which presents astringent properties. Its ethnopharmacological uses comprise, among others, anti-inflammatory and wound healing action, and it is used in the treatment of diarrhea and gynecological problems. The phytotherapeutic use of 'barbatimão' is largely related to its tannin content, which is abundant in its bark. The main goal of the present study was to evaluate the cytotoxic, mutagenic, and genotoxic potential of the lyophilized solution of the stem bark of S. adstringens, using the Ames test, the SOS-Inductest and the SOS-Chromotest. S. adstringens presented cytotoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stryphnodendron adstringens/cytotoxicity; Stryphnodendron adstringens/genotoxicity; Barbatimão/phytochemical study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400010

Oxybutynin and tolterodine for treatment of neurogenic detrusor overactivity: a pharmacoeconomic evaluation in the Brazilian context
Provedor de dados: 60
Autores: Suguino,Rodrigo Silva; Martins,Gisele; Campos,Brunna Carvalho Viana; Bessa,Raylayne Ferreira; Polli,Démerson André; Funez,Mani Indiana; Volpe,Cris Renata Grou.
Antimuscarinic agents are the first-line choice for the treatment of neurogenic detrusor overactivity (NDO). The currently available antimuscarinic drugs have been widely studied in patients presenting idiopathic detrusor overactivity; however, investigations evaluating the effects of these drugs on NDO are scant, particularly with regard to cost-effectiveness analyses. A pharmacoeconomic evaluation was performed to compare the costs and effectiveness of oxybutynin and tolterodine in two different formulations, extended (ER) and immediate-release (IR), for the treatment of NDO (based on Brazilian maximal consumer price index). A systematic review of literature was conducted in order to obtain significant clinical and urodynamic data (based on expert...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary Bladder Neurogenic; Muscarinic Antagonists; Pharmaceutical economy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200006

Safety and tolerability of a single dose T0001 in Chinese healthy adult volunteers: a first-in-human ascending dose study
Provedor de dados: 60
Autores: Zhang,Tan; Zhang,Minjie; Zu,Li’an; Wang,Qian; Wang,Qi; Wang,Wei; Wang,Yitong; Zang,Yannan; Xie,Zhenwei; Chen,Shi; Wang,Mei; Zheng,Qingshan; Li,Zhanguo; Chen,Guihong; Fang,Yi.
T0001 is the first mutant of etanercept with a higher affinity to tumor necrosis factor α (TNF-α) than etanercept. In order to investigate the safety and tolerability of T0001, a study was carried out in healthy Chinese subjects. A first-in-human, dose escalation study was conducted in healthy Chinese subjects. Fifty-six subjects were divided into six dose cohorts (10 mg, 20 mg, 35 mg, 50 mg, 65 mg and 75 mg) to receive a single subcutaneous injection of T0001. Safety and tolerability assessment were based on the records of vital signs, physical examinations, clinical laboratory tests, 12-lead electrocardiograms and adverse events (AEs). All subjects were in good compliance and none withdraw due to AEs. No serious AEs occurred. A total of twenty-three AEs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T0001; TNF-α; Rheumatoid arthritis; Safety; Tolerability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100598

Gender differences in biochemical markers and oxidative stress of rats after 28 days oral exposure to a mixture used for weight loss containing p-synephrine, ephedrine, salicin, and caffeine
Provedor de dados: 60
Autores: Schmitt,Gabriela Cristina; Arbo,Marcelo Dutra; Lorensi,Andréia Louise; Jacques,Ana Laura Bemvenuti; Nascimento,Sabrina Nunes do; Mariotti,Kristiane de Cássia; Garcia,Solange Cristina; Dallegrave,Eliane; Leal,Mirna Bainy; Limberger,Renata Pereira.
ABSTRACT The association of p-synephrine, ephedrine, salicin, and caffeine in dietary supplements and weight loss products is very common worldwide, even though ephedrine has been prohibited in many countries. The aim of this study was to evaluate a 28-day oral exposure toxicity profile of p-synephrine, ephedrine, salicin, and caffeine mixture (10:4:6:80 w/w respectively) in male and female Wistar rats. Body weight and signs of toxicity, morbidity, and mortality were observed daily. After 28 days, animals were euthanized and blood collected for hematological, biochemical, and oxidative stress evaluation. No clinical signs of toxicity, significant weight loss or deaths occurred, nor were there any significant alterations in hematological parameters....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-Synephrine/toxicity profile; Ephedrine/toxicity profile; Salicin/toxicity profile; Caffeine/toxicity profile; Dietary supplements/evaluation; Weight loss products/evaluation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100007

Evaluation of the proliferative activity of methanol extracts from six medicinal plants in murine spleen cells
Provedor de dados: 60
Autores: Zandonai,Rodrigo Hermes; Coelho,Fabiana; Ferreira,Juliana; Mendes,Ana Karla Bittencourt; Biavatti,Maique Weber; Niero,Rivaldo; Cechinel Filho,Valdir; Bueno,Ednéia Casagranda.
A number of natural compounds have been used as immunomodulatory agents, enabling the function of the immune system to be modified by stimulating or suppressing it. There has been increasing interest in the study of therapeutic action of plant extracts regarding their immunomodulatory activity. The aim of this study was to identify and evaluate the action of extracts of the medicinal plants Calophyllum brasiliense, Ipomoea pes-caprae, Matayba elaeagnoides, Maytenus robusta, Rubus imperialis and Vernonia scorpioides on the development of spleen cells from mice, using the in vitro cellular proliferation assay. The cells, obtained by mechanical rupture of mice spleen (5x10(4) cells/mL), were incubated with methanol extracts (10, 50, 100 and 200 µg/mL) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Natural products/immunomodulatories; Immunomodulation; Calophyllum brasiliense/pharmacognosy; Ipomoea pes-caprae/pharmacognosy; Matayba elaeagnoides/pharmacognosy; Maytenus robusta/pharmacognosy; Rubus imperialis/pharmacognosy; Vernonia scorpioides/pharmacognosy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200020

Urinary excretion of L-carnitine, acetyl-L-carnitine, propionyl-L-carnitine and their antioxidant activities after single dose administration of L-carnitine in healthy subjects
Provedor de dados: 60
Autores: Cao,Yu; Hao,Chuan-ji; Wang,Chen-jing; Li,Peng-li; Wang,Le-xin; Guan,Hua-shi; Li,Huan-ting.
The urine excretion of L-carnitine (LC), acetyl-L-carnitine (ALC) and propionyl-Lcarnitine (PLC) and their relations with the antioxidant activities are presently unknown. Liquid L-carnitine (2.0 g) was administered orally as a single dose in 12 healthy subjects. Urine concentrations of LC, ALC and PLC were detected by HPLC. Superoxide dismutase (SOD), total antioxidative capacity (T-AOC), malondialdehyde (MDA) and nitrogen monoxidum (NO) activities were measured by spectrophotometric methods. The 0~2 h, 2~4 h, 4~8 h, 8~12 h, 12~24 h excretion of LC was 53.13±31.36 µmol, 166.93±76.87 µmol, 219.92±76.30 µmol, 100.48±23.89 µmol, 72.07±25.77 µmol, respectively. The excretion of ALC was 29.70±14.43 µmol, 80.59±32.70 µmol, 109.85±49.21 µmol, 58.65±18.55 µmol,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-carnitine/antioxidant activity; Acetyl-L-carnitine/antioxidant activity; Propionyl-Lcarnitine/antioxidant activity; Antioxidants; Urine excretion/analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100020

Protective effect of mulberry flavonoids on sciatic nerve in alloxan-induced diabetic rats
Provedor de dados: 60
Autores: Song-Tao,Ma; Dong-lian,Liu; Jing-jing,Deng; Yan-juan,Peng.
Mulberry leaves (Morus alba L.) are a traditional Chinese medicine for blood serum glucose reduction. This study evaluated the protective effects of mulberry flavonoids on sciatic nerve in alloxan-induced diabetic rats. In this study, 80 Sprague-Dawley rats were divided into five groups: A (control), B (diabetic treated with saline), C-D (diabetic treated with 0.3, 0.1 g/kg mulberry flavonoids once a day for 8 weeks) and E (diabetic treated with 0.3 mg/kg methycobal). The diabetic condition was induced by intraperitoneal injection of 200 mg/kg alloxan dissolved in saline. At the end of the experimental period, blood, and tissue samples were obtained for biochemical and histopathological investigation. Treatment with 0.3 g/kg mulberry flavonoids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morus albaL./pharmacognosy; Mulberry flavonoids/protective effects/experimental study; Diabetic neuropathy/experimental study; Medicinal plants; Diabetics/treatment/experimental study.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400765

Atorvastatin calcium loaded chitosan nanoparticles: in vitro evaluation and in vivo pharmacokinetic studies in rabbits
Provedor de dados: 60
Autores: Ahmed,Abdul Baquee; Konwar,Ranjit; Sengupta,Rupa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atorvastatin calcium/oral bioavailability/experimental study; Atorvastatin calcium/in vitro release; Atorvastatin calcium/pharmacokinetics; Nanoparticles/drugs bioavailability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200467

Enteric coating of ibuprofen tablets (200 mg) using an aqueous dispersion system
Provedor de dados: 60
Autores: Bushra,Rabia; Shoaib,Muhammad Harris; Aslam,Nousheen; Mehmood,Zafar Alam; Hashmat,Durriya.
Ibuprofen is a propionic acid derivative that belongs to the class NSAIDs. Major adverse reactions associated with Ibuprofen are related to GIT and include peptic and mucosal ulcers, dyspepsia, severe gastric pain and bleeding, that results in excessive treatment failure. The goal of this study was to develop enteric coated ibuprofen tablets in order to avoid gastric mucosal irritation, diffusion of drug across mucosal lining and to let active ingredient be absorbed easily in small intestine. The formulation was developed and manufactured through the direct compression process, the simplest, easiest and most economical method of manufacturing. Enteric coating was done using an Opadry white subcoating and an aqueous coating dispersion of Acryl-Eze. Enteric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ibuprofen/adverse reactions; Tablets/enteric coating/aqueous dispersion system; Acryl-Eze. Opadry White; Superdisintegrant.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100011

Evaluation of recombinant human interferon beta 1b by liquid chromatography methods and bioassay
Provedor de dados: 60
Autores: Silva,Francielle Santos da; Walter,Maurício Elesbão; Xavier,Bruna; Perobelli,Rafaela Ferreira; Calegari,Guilherme Zanini; Cardoso,Douglas Franco; Perlin,Valquiria Guedes; Dalmora,Sérgio Luiz.
Recombinant human interferon beta 1b (rhIFNβ-1b) is clinically used to treat multiple sclerosis. A reversed-phase liquid chromatography (RP-LC) method was carried out on a Jupiter C4 column (250 mm × 4.6 mm i.d.). The mobile phase A consisted of 0.1% trifluoroacetic acid (TFA) in water, and the mobile phase B was acetonitrile with 0.1% TFA run at a flow rate of 1.0 mL/min. A size exclusion liquid chromatography (SE-LC) method was carried out on a BioSep-SEC-S 2000 column (300 mm × 7.8 mm i.d.). The mobile phase consisted of 1 mM monobasic potassium phosphate, 8 mM sodium phosphate dibasic and 200 mM sodium chloride buffer pH 7.4, run isocratically at a flow rate of 0.8 mL/min. Retention times were 31.87 and 17.78 min, and calibration curves were linear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant human Interferon beta 1b; Biotechnology-derived medicine; Bioassay; Reversed-phase liquid chromatography; Size-exclusion liquid chromatography.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100571

Stability evaluation of organic Lip Balm
Provedor de dados: 60
Autores: Fernandes,Alessandra Ribeiro; Dario,Michelli Ferrera; Pinto,Claudinéia Aparecida Sales de Oliveira; Kaneko,Telma Mary; Baby,André Rolim; Velasco,Maria Valéria Robles.
Rising global demand for natural products whose production is harmless to the environment has stimulated the development of natural cosmetics and, within this category, organics (95% organic raw materials). The image of environmentally friendly production is one of the strongest attractions of organic products. Lip balm is a cosmetic product similar to lipstick whose purpose is to prevent lip dryness and protect against adverse environmental factors. The product's characteristics are: resistance to temperature variations, pleasant flavor, innocuousness, smoothness during application, adherence and easy intentional removal. This work involved the development of a lip balm formulated with certified organic raw materials and the execution of stability tests:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lip balm; Stability; Cocoa butter; Cosmetic; Organic.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200011

Anti-inflammatory and healing action of oral gel containing borneol monoterpene in chemotherapy-induced mucositis in rats ( Rattus norvegicus )
Provedor de dados: 60
Autores: Nascimento-Júnior,Braz José do; Brito,Lucas de Souza; Barros,Walquíria Nunes; Gonçalves,Daniela Marques; Matos,Luana de Souza; Nascimento,Cínthia Reyjane Borges; Ribeiro,Luciano Augusto de Araújo; Lima,Ricardo Santana de; Silva-Júnior,René Geraldo Cordeiro; Reis,Sílvio Alan Gonçalves Bomfim; Gonçalves,Talita Mota; Amorim,Elba Lúcia Cavalcanti de.
ABSTRACT The aim of this study was to investigate the effect of gels containing the monoterpene borneol in induced oral mucositis using an animal model. Gels were prepared with borneol at 1.2% and 2.4% (w/w). Oral mucositis was induced by administration of three doses of 5-fluorouracil (30 mg/kg, i.p.) and injury with acetic acid (50%, v/v) soaked in filter paper applied to right cheek mucosa for 60s. Four subgroups comprising 12 animals each were formed. Six animals from each group were sacrificed at days seven and fourteen after oral mucositis induction. Mucous samples were processed and stained with hematoxylin-eosin and Masson’s Trichrome. The semiquantitative evaluation involved observation of inflammatory parameters. ImageJ® software was used in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemotherapy; Oral gel/Borneol monoterpene/effects; Oral gel/Borneol monoterpene/action anti-inflammatory; Oral gel/Borneol monoterpene/healing action; Bornyl alcohol/effects; Palliative care.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300604

Analgesic and antiinflammatory activities of the essential oil from Artemisia sieberi Besser
Provedor de dados: 60
Autores: Pishgahzadeh,Elahe; Shafaroodi,Hamed; Asgarpanah,Jinous.
The analgesic activity of Artemisia sieberi oil was assessed by acetic acid-induced writhing test and Eddy’s hot plate method; while the acute anti-inflammatory effect was investigated by inflammatory paw edema test in rats. The administration rout of the essential oil, standard drugs and the vehicle used in all assays was intraperitoneal injection. The 1 and 2.5 mg/kg doses of the studied oil significantly decreased the number of acetic acid-induced writhes in mice. The dose of 1 mg/kg of the oil also exhibited a central analgesic effect as evidenced by a significant increase in reaction time at several time points after 15 min treatment in the hot plate method. In addition, the 1 mg/kg dose of the oil significantly reduced carrageenan induced paw edema...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemisia sieberi drug effects Essential oil/therapeutic use Volatile oils/therapeutic use Analgesics/administration and dosage Anti-Inflammatory Agents Administration &amp;amp; Dosage.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100579

Development and validation of a HPTLC method for analysis of Sunitinib malate
Provedor de dados: 60
Autores: Hajmalek,Monireh; Goudarzi,Masoumeh; Ghaffari,Solmaz; Attar,Hossein; Mazlaghan,Mehrnoosh Ghanbari.
ABSTRACT A simple high performance thin layer chromatography (HPTLC) has been developed and validated for determination of sunitinib malate and possible impurities. The samples were applied in forms of bands on an aluminum TLC plate pre-coated with silica gel and were separated using dichloromethane: methanol: toluene: ammonia solution as the mobile phase. Sunitinib malate was thoroughly separated from impurities including E-isomer, sunitinib N-oxide and impurity B with a retention factor (RF) of 0.35±0.02. Quantitative analysis of sunitinib was carried out using a mobile phase consisting of dichloromethane:methanol:ammonia solution, RF value was 0.53±0.02 for Z isomer. Detection was performed densitometrically in absorbance mode at 430 nm. This method was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sunitinib malate/quantitative analysis; High Performance Thin Layer Chromatography/method/validation; Sunitinib malate/E-Z isomer.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400595

Use of solid dispersions to increase stability of dithranol in topical formulations
Provedor de dados: 60
Autores: Estanqueiro,Marilene; Conceição,Jaime; Amaral,Maria Helena; Lobo,José Manuel Sousa.
The present study was planned to improve the stability of dithranol using solid dispersions (SD). Two different SD at a 1:9 ratio of dithranol/excipient were prepared: one of them using glyceryl behenate as excipient and the other using a mixture of argan oil with stearic acid (1:8 ratio) as excipient. Pure dithranol and SD of dithranol were incorporated in an oil-in-water cream and in a hydrophobic ointment in a drug/dermatological base ratio of 1:10. The physical and mechanical properties of semisolid formulations incorporating the pure drug and the developed SD were evaluated through rheological and textural analysis. To evaluate the stability, L*a*b* color space parameters of SD and semisolid formulations, and pH of hydrophilic formulations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dithranol/stability; Topical formulations/development; Solid dispersions/preparation; Stearic acid; Glyceryl behenate; Argan oil.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300583

Quality by design (Qbd) approach to develop stability indicating HPLC method for estimation of rutin in chitosan-sodium alginate nanoparticles
Provedor de dados: 60
Autores: Vijayaraj,Surendran; Palei,Narahari N; Archana,Devalapalli; Lathasri,Kuppam; Rajavel,Ponnusamy.
Rutin is a flavonoid glycoside, mainly consists of phenolic compounds, responsible for many biological activities. The objective of the present study was to develop and validate a precise, simple, robust, rapid and reliable reverse phase high -performance liquid chromatography (RP-HPLC) technique by using Qbd approach for evaluating the rutin in nanoparticles. Central composite design (CCD) was employed for optimizing the experimental conditions of RP-HPLC method. Buffer pH, methanol content in the mobile phase composition, flow rate, and wavelength were selected as independent variables whereas retention time, peak area, and asymmetry factor was selected as dependent variables. The retention time, peak area and asymmetric factor of rutin by using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rutin; Chitosan; Sodium aliginate; Nanoparticles; RP-HPLC; Central composite design; QbD.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100632

Beating drug tests and defending positive results: a toxicologist's perspective
Provedor de dados: 60
Autores: Yonamine,Mauricio.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400033

Preparation and characterization of a novel antimicrobial film dressing for wound healing application
Provedor de dados: 60
Autores: Gonçalves,Melissa Marques; Carneiro,Jaqueline; Justus,Barbara; Espinoza,Joel Toribio; Budel,Jane Manfron; Farago,Paulo Vitor; Paula,Josiane Padilha de.
Antibacterial activity and good mechanical properties are some of the characteristics required for an appropriate film dressing. A novel polymer blend was developed for wound healing application. Twenty-four formulations using the polymers chitosan, poly(vinyl alcohol) and/or ɛ-Polylysine and the plasticizer glycerol were designed using factorial design and then the films were prepared by the casting/solvent evaporation method. Seventeen films were obtained among the twenty-four proposed formulations that were characterized by Field Emission Scanning Electron Microscopy (FE-SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Mechanical properties, such as tensile strength (σ), elongation at break (ɛ) and Young’s modulus (Y) as well as antibacterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitosan; Poly(vinyl alcohol); Ɛ-Polylysine; Semi-occlusive dressing.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100631

Libraries and librarians as coadjutants in the editorial process of scientific publications - reaffirming a proposal
Provedor de dados: 60
Autores: Aranha,Leila Rangel de Carvalho.
Tipo: Info:eu-repo/semantics/other
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400001

Extracts from Amazonian plants have inhibitory activity against tyrosinase: an in vitro evaluation
Provedor de dados: 60
Autores: Macrini,Daclé Juliani; Suffredini,Ivana Barbosa; Varella,Antonio Drauzio; Younes,Riad Naim; Ohara,Mitsuko Taba.
Dermatological disorders related to pigmentation result in tenuous hyper or hypopigmentation Cosmetic and pharmaceutical products containing depigmenting substances are used in the treatment of patients who have high pigmentation disorders, such as melasma or chloasma, post-inflammatory hyperpigmentation, senile lentigo and ephelides. Skin lightening agents are not yet totally effective or safe and therefore intensive research for the discovery of new agents is continuous. Enzyme inhibitors involved in melanogenesis, such as tyrosinase, have been discovered in Asian countries, including those isolated from plant extracts. The Brazilian flora has the highest species diversity in the world, and the chemical, pharmacological and cosmetic potential for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tyrosinase; Melanin; Plant extract/in vitro evaluation; Skin lightening agents; Amazonian plants/evaluation/dermatological use.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400015

Emerging intervention of antidepressant with DMARD in non-cancerous nociceptive persistent pain associated depression in FCA induced rheumatoid arthritic rats
Provedor de dados: 60
Autores: Pachiappan,Sudhakar; Murugaiyan,Sudaroli; Chandrasekar,Sabarinath.
Rheumatoid arthritis (RA) is a chronic inflammatory disorder that causes pain, systemic complications and premature mortality. Depression has also been identified as a problem for persons with RA. This association remaining significant even after the degree of disease activity is controlled. In the present study, the efficacy of combination therapeutic effect of antidepressant (amitriptyline) with Disease Modifying Anti rheumatoid drug (leflunomide) was determined in rheumatoid arthritis pain associated depression in Freund’s complete adjuvant (FCA) induced arthritic rats. Drug treatment was started 9 days after induction of FCA induced arthritis in rats. The antiarthritic activity was assessed by measuring paw volume, weight-bearing, hematological,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthritis associated depression; Freund’s complete adjuvant; Amitriptyline; Leflunomide.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100637

Evaluation of foam properties of saponin from Ilex paraguariensis A. St. Hil. (Aquifoliaceae) fruits
Provedor de dados: 60
Autores: Canto,Gizele Scotti do; Treter,Janine; Yang,Samanta; Borré,Gustavo Luís; Peixoto,Maria Paula Garofo; Ortega,George González.
Saponins are natural compounds able to form abundant foam, a desirable quality required in some chemical, foods, cosmetic and pharmaceutical processes. Ilex paraguariensis A. St. Hil. (Aquifoliaceae) known as mate, is a South American widely cultivated specie due to the preparation of a tea-like beverage from its leaves. Moreover, its green fruits are a rich source of non-toxic and very low haemolytic saponins. In this study, mate saponin fraction (MSF) was evaluated as a foam former, focusing on its foamability, foam lifetime, and film drainage in the presence of different electrolytes (ionic strength I = 0.024 M). Sodium dodecyl sulphate (SDS) and polysorbate 80 (Poly-80) were used as reference surfactants. The critical micelle concentration (CMC), the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis; Aquifoliaceae; Mate; Saponins; Surfactants; Foam; Assay.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200010

Appraisal of acute oral toxicity of glucuronoxylan hydrogel from Mimosa pudica seeds
Provedor de dados: 60
Autores: Muhammad,Gulzar; Hussain*,Muhammad Ajaz; Ashraf,Muhammad Umer; Naeem-ul-Hassan,Muhammad; Bukhari,Syed Nasir Abbas.
Glucuronoxylan hydrogel (GXH) isolated from M. pudica seeds was assessed for acute toxicology in albino mice that were alienated into four groups. Three groups, i.e., II, III and IV received GXH at a dose of 1, 2 and 5 g/kg, respectively while group I was retained untreated and provided routine diet. After administering GXH, mice were examined for vomiting, diarrhea, allergy and tremors for 8 h. All animals were carefully observed for food and water consumption at 1, 2, 3, 7 and 14 day after administering GXH. At the end of studies, blood samples were drawn for investigation of hematological and biochemical parameters. All animals were sacrificed, relative body weight of vital organs was calculated and their histopathology was studied. It was concluded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucuronoxylan; Acute toxicity; Histopathology; Toxicology; Mimosa pudica.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300619

Antibacterial evaluation of Styrax pohlii and isolated compounds
Provedor de dados: 60
Autores: Bertanha,Camila Spereta; Utrera,Susane Hellen; Gimenez,Valéria Maria Melleiro; Groppo,Milton; Silva,Márcio Luis Andrade e; Cunha,Wilson Roberto; Martins,Carlos Henrique Gomes; Januário,Ana Helena; Pauletti,Patrícia Mendonça.
The antibacterial activity of the compounds egonol (1) and homoegonol (2), of the crude ethanolic extract of Styrax pohlii (Styracaceae) aerial parts (EE), and of its n-hexane (HF), EtOAc (EF), n-BuOH (BF), and hydromethanolic (HMF) fractions was evaluated against the following microorganisms: Streptococcus pneumoniae (ATCC 6305), S. pyogenes (ATCC 19615), Haemophilus influenzae (ATCC 10211), Pseudomonas aeruginosa (ATCC 27853), and Klebsiella pneumoniae (ATCC 10031). The broth microdilution method was used for determination of the minimum inhibitory concentration (MIC) during preliminary evaluation of antibacterial activity. The EE yielded MIC values of 400 µg/mL for S. pneumoniae and P. aeruginosa and 300 µg/mL for H. influenzae. The HF and EF fractions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Styrax pohlii/pharmacognosy; Styracaceae/pharmacognosy; Styrax pohlii/ethanolic extract; Styrax pohlii/antibacterial activity; Egonol/antibacterial activity; Homoegonol/antibacterial activity; Natural products/evaluation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400004

Formulation development and evaluation of colon targeted delayed release methotrexate pellets for the treatment of colonic carcinoma
Provedor de dados: 60
Autores: Paradkar,Mansi; Amin,Jinal.
Colonic carcinoma is one of the most common internal malignancies and is the second leading cause of deaths in United States. Methotrexate (MTX) is a drug of choice in the treatment of colon cancer. The aim of the present research work was to develop and characterize colon targeted pellets of MTX for treatment of colonic carcinoma. The product and process parameters were optimized by screening methods. Pellets were prepared by extrusion spheronization using microcrystalline cellulose (MCC) as spheronizing aid and ethyl cellulose (EC) as release retardant in different ratio. Based on the physical appearance, sphericity and % in vitro drug release, batch P17 containing EC: MCC (3:7) was optimized for core pellets. The site specificity was obtained by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethyl cellulose; Microcrystalline cellulose; Eudragit S100; Extrusion-spheronization; Modified fluid bed coater; Ex-vivo drug release study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400608

Convergent synthesis of new N -substituted 2-{[5-(1H -indol-3-ylmethyl)-1,3,4-oxadiazol-2-yl]sulfanyl}acetamides as suitable therapeutic agents
Provedor de dados: 60
Autores: Rubab,Kaniz; Abbasi,Muhammad Athar; Aziz-ur-Rehman,; Siddiqui,Sabahat Zahra; Ashraf,Muhammad; Shaukat,Ayesha; Ahmad,Irshad; Lodhi,Muhammad Arif; Khan,Farman Ali; Shahid,Muhammad; Akhtar,Muhammad Nadeem.
abstract A series of N-substituted 2-{[5-(1H-indol-3-ylmethyl)-1,3,4-oxadiazol-2-yl]sulfanyl}acetamides (8a-w) was synthesized in three steps. The first step involved the sequential conversion of 2-(1H-indol-3-yl)acetic acid (1) to ester (2) followed by hydrazide (3) formation and finally cyclization in the presence of CS2 and alcoholic KOH yielded 5-(1H-indole-3-yl-methyl)-1,3,4-oxadiazole-2-thiol (4). In the second step, aryl/aralkyl amines (5a-w) were reacted with 2-bromoacetyl bromide (6) in basic medium to yield 2-bromo-N-substituted acetamides (7a-w). In the third step, these electrophiles (7a-w) were reacted with 4 to afford the target compounds (8a-w). Structural elucidation of all the synthesized derivatives was done by 1H-NMR, IR and EI-MS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 1H-indol-3-acetic acid N-substituted 2-{[5-(1H-indol-3-ylmethyl)-1; 3; 4-oxadiazol-2-yl] sulfanyl}acetamides/antibacterial activity N-substituted 2-{[5-(1H-indol-3-ylmethyl)-1; 3; 4-oxadiazol- 2-yl]sulfanyl} acetmides/hmolytic activity α-Glicosidase Butirylcholinesterase Lipoxygenase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400931

Corrositex®, BCOP and HET-CAM as alternative methods to animal experimentation
Provedor de dados: 60
Autores: Cazedey,Edith Cristina Laignier; Carvalho,Flávia Chiva; Fiorentino,Flávia Angélica Másquio; Gremião,Maria Palmira Daflon; Salgado,Hérida Regina Nunes.
Tests in animals are used as models in toxicological and investigative studies. However, such tests have been considered inhumane because they can cause pain and suffering to experimental animals, while these methods can often be subjective. Protests calling for animal protection have questioned the effectiveness of in vivo tests and suggest the introduction of alternative, in vitro methods. International organizations, such as the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the National Institute of Health (NIH), the Organization for Economic Co-operation and Development (OECD), that regulate and develop new alternative animal models, have indicated the running of preliminary assays and execution of sequential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal experimentation/alternative methods in vitro; Experiments/in vitro methods; Corrositex®/in vitro method/experiments; BCOP/in vitro method/experiments; HET-CAM/in vitro method/experiments.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400021

Green tea glycolic extract-loaded liquid crystal systems: development, characterization and microbiological control
Provedor de dados: 60
Autores: Bernegossi,Jéssica; Barbosa,Raquel Maria Cunha; Rustice,Patrícia Maciel; Chorilli,Marlus.
ABSTRACT Liquid crystal systems (LCSs) have interesting cosmetic applications because of their ability to increase the therapeutic efficiency and solubility of active ingredients. The aim of the present research was to develop green tea glycolic extract-loaded LCSs, to characterize and to perform microbiological control. The ternary phase diagram was constructed using polysorbate 20, silicone glycol copolymer (SGC) - DC 193(r), and distilled water with 1.5% glycolic green tea extract. The systems were characterized by polarized light microscopy. Formulations selected were characterized as transparent viscous systems and transparent liquid system indicated mesophases lamellar structure. The results of the microbiological analysis of mesophilic aerobic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Camellia sinensis/glycolic extract; Liquid crystal systems/cosmetic applications; Liquid crystal systems/characterization; Liquid crystal systems/microbiological control.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300383

Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis
Provedor de dados: 60
Autores: Cârcu-Dobrin,Melania; Sabău,Alexandra Georgiana; Hancu,Gabriel; Árpád,Gyéresi; Rusu,Aura; Kelemen,Hajnal; Papp,Lajos Attila; Cârje,Anca.
The present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 ºC, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amlodipine; Capillary electrophoresis; Chiral separation; Cyclodextrine; Experimental design.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100530

Food
Provedor de dados: 60
Autores: Marques,Úrsula M. Lanfer.
Tipo: Info:eu-repo/semantics/review
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100027

Evaluation of the satisfaction level of patients attended by a pharmaceutical care program in a private communitarian pharmacy in Vitória (ES, Brazil)
Provedor de dados: 60
Autores: Andrade,Tadeu Uggere de; Burini,Daiani Mucelin; Mello,Michelli de Oliveira; Bersácula,Natyara dos Santos; Saliba,Renata Aubin Dias; Bravim,Francielli Teixeira; Bissoli,Nazaré Souza.
The present study was designed to evaluate the satisfaction of users of a Pharmaceutical Care (PC) service in a private communitarian pharmacy in Vitória (ES, Brazil). In this transversal observational study, patient interviews were performed by an experimenter that had no relationship with the establishments evaluated. Data were collected using a structured questionnaire. The questionnaire used a five point Likert scale, in which smaller numbers represented lower levels of satisfaction. For comparison, user satisfaction was also evaluated for two pharmaceutical establishments that do not have standardized PC services. In these cases, age-matched patients were selected randomly. A higher level of satisfaction was reported by users of the PC service, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care/user´s evaluation; Pharmacy services; Communitarian pharmacy/evaluation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200021

Design and optimization of self-microemulsifying drug delivery system (SMEDDS) of felodipine for chronotherapeutic application
Provedor de dados: 60
Autores: Ansari,Khalid Akhter; Pagar,Kunal Pratap; Anwar,Shoeb; Vavia,Pradeep Ratilal.
The objective of this research work was to design, develop and optimize the self micro-emulsifying drug delivery system (SMEDDS) of Felodipine (FL) filled in hard gelatine capsule coated with polymer in order to achieve rapid drug release after a desired time lag in the management of hypertension. Microemulsion is composed of a FL, Lauroglycol FCC, Transcutol P and Cremophor EL. The optimum surfactant to co-surfactant ratio was found to be 2:1. The resultant microemulsions have a particle size in the range of 65-85 nm and zeta potential value of -13.71 mV. FL release was adequately adjusted by using pH independent polymer i.e. ethyl cellulose along with dibutyl phthalate as plasticizer. Influence of formulation variables like viscosity of polymer, type of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Felodipine/self-microemulsifying delivery; Felodipine/chronotherapeutic application; Self-microemulsifying drug delivery system/development; Drugs/controlled release; Ethyl cellulose/drugs coated; Hypertension/treatment; Chronotherapeutics/hypertension treatment.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100021

Effect of stearic acid on the properties of metronidazole/methocel K4M floating matrices
Provedor de dados: 60
Autores: Lara-Hernández,Belem; Hernández-León,Alejandra; Villafuerte-Robles,Leopoldo.
The properties of metronidazole/Methocel K4M sustained release floating tablets have been studied varying the proportion of the lubricant, stearic acid, on formulations with and without sodium bicarbonate. The variables studied include technological properties of the tablets such as tablet hardness and ejection pressure, the drug release profile, the hydration kinetics and the floating behaviour. The presence of stearic acid and sodium bicarbonate improves the floating behaviour for more than 8 hours. The hydration volume, the tablet hardness and the ejection pressure decrease as the stearic acid content increases and the polymer content decreases. Drug dissolution increases with increasing proportions of stearic acid and decreasing proportions of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metronidazole; Methocel K4M; Stearic acid; Matrices hydration; Gastric retention; Sodium bicarbonate; Sustained release; Drugs/release mechanism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300016

Liposomes: methods and protocols
Provedor de dados: 60
Autores: Chacra,Nádia Araci Bou.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400029

Anti- Sporothrix spp. activity of medicinal plants
Provedor de dados: 60
Autores: Waller,Stefanie Bressan; Madrid,Isabel Martins; Faria,Renata Osório de; Cleff,Marlete Brum; Mello,João Roberto Braga de; Meireles,Mário Carlos Araújo.
ABSTRACT Cases of sporotrichosis in humans and animals without satisfactory clinical response have increased, a warning sign of strains resistant to conventional antifungal agents. The urgent search for alternative therapies was an incentive for research on medicinal plants with anti-Sporothrix spp. properties. A bibliographic survey was performed based on scientific papers about in vitro and in vivo antifungal activity of essential oils and extracts of plants in differents solvents against the fungal of the Sporothrix schenckii complex. The study methodology consisted of a literature review in Google Scholar, Science Direct, Pubmed, Bireme and Springer link with papers from 1986 to 2015. We found 141 species of plants that were investigated, of which 100...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sporothrix schenckii complex; Antifungals; Combretaceae/pharmacognosy; Asteraceae/pharmacognosy; Lamiaceae/pharmacognosy; Medicinal plants/antifungal activity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200221

The radiation protection role of heparin-SOD conjugate in irradiated mice
Provedor de dados: 60
Autores: Zhong,Chuanqing; Huang,Xiaojing; Zhang,Shu; Cheng,Yanna; Cao,Guangxiang; Wang,Fengshan.
ABSTRACT Heparin-SOD conjugate (Hep-SOD) was prepared by modifying Cu,Zn-SOD with heparin. An acute radiation-induced mouse injury model was constructed to study the radiation protection effects of Hep-SOD conjugate. Fifty-six mice were randomly divided into seven groups: (I) normal control group; (II) irradiated control group; (III) positive control group (amifostine group, 300 mg/kg); (IV) SOD group (35000 U/kg); (V) high dosage of Hep-SOD group (70000 U/kg); (VI) medium dosage of Hep-SOD group (35000 U/kg); (VII) low dosage of Hep-SOD group (17500 U/kg). Drugs were intraperitoneally injected into each mouse 1 h before radiation except for the normal control group. All the irradiated groups were irradiated with 6 Gy. Organ indices, haematopoietic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparin-superoxide dismutase conjugate; Radiation-induced damage; Oxidative stress injury.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400611

Influence of the clinical profile of patients with refractory epilepsy on lamotrigine plasma concentration
Provedor de dados: 60
Autores: Baldoni,André Oliveira; Freitas-Lima,Priscila; Alexandre,Veriano; Ferreira,Flávia Isaura de Santi; Martinez,Edson Zangiacomi; Queiroz,Regina Helena Costa; Sakamoto,Americo Ceiki; Pereira,Leonardo Regis Leira.
ABSTRACT The purpose of this work was to evaluate the influence of the clinical profile on lamotrigine (LTG) plasma concentrations from patients with refractory epileptic seizures. In this cross-sectional study, therapeutic monitoring of LTG, and questionnaires with 75 patients with refractory epileptic seizures of a Hospital in Ribeirão Preto-SP-Brazil were performed. The multiple linear regression model was used to verify association between the LTG plasma concentrations and the independent variables. Covariance analysis was used to compare the mean LTG plasma concentration among the co-medication groups. The LTG plasma concentration was associated both with the LTG dosage (mg/kg/day) (p=0.0096) and with the use of first generation antiepileptic drugs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lamotrigine; Refractory Epilepsy; Drug interaction; Therapeutic drug monitoring.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200624

Complexation and enhancement of temozolomide solubility with cyclodextrins
Provedor de dados: 60
Autores: Gürten,Berna; Yenigül,Elçin; Sezer,Ali Demir; Malta,Seyda.
ABSTRACT Temozolomide is a poorly soluble anti-cancer drug used in the treatment of some brain cancers. Following literature reports about the enhancement of solubility and stability for these kinds of drugs upon complexation with cyclodextrins, we aimed to form an inclusion complex between temozolomide and the different types of cyclodextrins (CDs) to enhance its solubility. In this study, three different cyclodextrins (β -CD, hydroxyl-β-CD and γ-CD) were used, and changes in solubility was measured by UV-Vis Spectroscopy and HPLC. Morphological changes upon complexation were shown by the Scanning Electron Microscope (SEM), and weight loss profiles with respect to temperatures which were unique to the compounds were shown by Thermogravimetric Analysis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temozolomide/solubility/complexation; Cyclodextrins; Chromatography; Calorimetry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200606

Prevalence and risk of potentially adverse drug interactions in the treatment of acute alcohol poisoning
Provedor de dados: 60
Autores: Andrade,Joyce Dalline Silva; Rocha,Chiara Erminia; Maciel,Maria Amélia Vieira; Santana,Danielle Cristine Almeida Silva de; Santana,Fernando José Malagueño de.
ABSTRACT The aim of this paper is to determine the profile of acute alcohol poisoning and to estimate the risk of potentially adverse drug interactions (ADIs) in patients intoxicated by alcohol when attended in emergency care at hospital. A descriptive serial cross-sectional study was performed with 4,271 individuals intoxicated by alcohol, from January 2009 to July 2011. Possible correlations were measured by Pearson's chi-square test. The data show high consumption in the population, especially in males between 25 and 59 years. The main circumstances for poisoning were alcohol misuse (96.3%). After treatment complete recovery from the signs or symptoms of the poisoning was observed in 96.88% cases; and death in 0.70%. The demonstration of potential risk...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholic intoxication; Emergency Medical Services; Risk Management; Medicines/adverse interactions/risk control; Medicines/alcohol interactions/risk control.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100015

In vitro evaluation of copaiba oil as a kojic acid skin enhancer
Provedor de dados: 60
Autores: Oliveira,Robson Vicente Machado de; Ohara,Mitsuko Taba; Vila,Marta Maria Duarte Carvalho; Gonçalves,Marcos Moisés.
The capacity of copaíba oil to act as a skin penetration enhancer for the depigmenting agent kojic acid was evaluated using an in vitro diffusion system with static flux and shed rattlesnake skin membrane, Crotalus durissus terrificus, in saline solution at 34±2 ºC as the fluid receptor. The quantities of kojic acid liberated into the fluid receptor were determined by spectrophotometry at 268 nm with intervals of one and a half hours. The membranes, pretreated with copaíba oil at 25% and 50% v/v, gave flux values of 8.0 and 12.7 µg/cm²/h, permeability values of 2.0 and 3.3 cm×10-4/h, and promotion factors of 4.1 and 3.7, respectively. These results indicate that copaíba oil, at the two concentrations studied, has the capacity to promote penetration of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copaíba oil; Kojic acid. Skin penetration/in vitro studies; UV Spectrophotometry.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200024

Formulation and in vitro evaluation of taste-masked oro-dispersible dosage form of diltiazem hydrochloride
Provedor de dados: 60
Autores: Jagdale,Swati Changdeo; Gawali,Vaibhav Uttam; Kuchekar,Bhanudas Shankar; Chabukswar,Aniruddha Rajaram.
Diltiazem hydrochloride is a calcium channel blocker generally indicated for the treatment of angina and hypertension, and it is extensively metabolized due to the hepatic metabolism. Formulation of diltiazem hydrochloride into an oro-dispersible dosage form can provide fast relief with higher bioavailability. The bitter taste of the drug should be masked to formulate it in a palatable form. In the present work, an attempt was made to mask the taste by complexation technique, with a formulation into an oro-dispersible dosage form, using superdisintegrants Doshion P544, crospovidone (CP) and sodium starch glycolate (SSG). The complexes of diltiazem hydrochloride with β-CD (1:1 molar ratio) were prepared by kneading, co-evaporation, co-grounding,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diltiazem hydrochloride; Cyclodextrin; Drugs/taste-masking; Freeze-drying.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400028

Validated analytical study of the effect of Lycopene on the pharmacokinetics of Paracetamol and Chlorzoxazone in rats
Provedor de dados: 60
Autores: Youssef,Reem Baha-Aldeen; Fouad,Marwa Ahmed; El-Zaher,Asmaa Ahmed.
Lycopene was reported to influence some cytochrome P450 enzymes activity. The present study investigates the effect of lycopene on the pharmacokinetics of paracetamol and chlorzoxazone. Lycopene (20 mg/kg) was intra-peritoneally administered to two groups of rats for eight consecutive days and two other groups were given vehicle. On the eighth day, chlorzoxazone and paracetamol were separately intravenously administered to a lycopene group and a control group. Blood samples were collected at different time intervals, treated and analyzed using HPLC. The HPLC method used for paracetamol analysis was based on isocratic elution using a mobile phase consisting of water: methanol, (77:23 v/v) at a flow rate 1 mL min−1, Kromasil C18 column, and UV detection at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopene/ chemistry; Paracetamol/pharmacokinetics; Chlorzoxazone/pharmacokinetics; Acetaminophen/pharmacokinetics; AUC; Area under curve; Volume of distribution; Validation studies; Metabolic clearance rate/drug effects; HPLC/methods; Chromatography; High Pressure Liquid/methods; Rats/metabolism.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100540

Central activities of hydroalcoholic extract from Lafoensia pacari A. St.-Hil. stem bark
Provedor de dados: 60
Autores: Galdino,Pablinny Moreira; Nascimento,Marcus Vinícius Mariano; Sousa,Fábio Borges de; Ferreira,Reginaldo Nassar; Paula,José Realino de; Costa,Elson Alves.
Lafoensia pacari A. St.-Hil. can be found from Amapá to Rio Grande do Sul states, and also in Paraguay and Bolivia. It is popularly known as pacari or mangava-brava and is used to promote weight loss, as an anti-thermal or tonic, to treat gastritis, ulcers, scarring, itching, discouragement, and cancer. In the open field tests, the hydroalcoholic extract from L. pacari stem bark (HEP) decreased the number of rearings, number of invaded squares, and increased immobility time compared to control animals. In the pentobarbital-induced sleep time test, HEP decreased latency time to sleep and increased sleeping time. In the rota-rod test, no changes in the studied parameters were observed. In the elevated plus maze, HEP increased the percentage time and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lafoensia pacari A. St.-Hil/pharmacognosy; Hydroalcoholic extract; Central Nervous System/experimental study; Anxiety/experimental study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300008

Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
Provedor de dados: 60
Autores: Erhirhie,Earnest Oghenesuvwe; Ilodigwe,Emmanuel Emeka.
This study evaluated the acute and sub-chronic toxicities of ethanol leaf extract of Dryopteris filix-mas. Acute toxicity and phytochemical tests on ethanol leaf extract were determined. In sub-chronic toxicity test, animals were treated with 62.5, 125, 250 and 500 mg/kg of extract every day for 90 days. Blood samples were collected via retro-orbital puncture for baseline studies and at 31, 61 and 91st days for determination of hematological, kidney and liver function parameters. Liver and kidneys were harvested for histopathology analyses on 91st day. Also, a 28 day recovery study was carried out to determine reversibility in toxicological effects. Phytochemical screening revealed the presence of tannins, phenols, flavonoids, saponins, steroids,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dryopteris filix mas Leaf Extract; Sub-chronic toxicity; Acute toxicity; Nephrotoxicity; Hepatotoxicity; Dryopteris/toxicity; Toxicity tests/utilization; Ethanol/ toxicity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100543

Implants as drug delivery devices for the treatment of eye diseases
Provedor de dados: 60
Autores: Silva,Gisele Rodrigues da; Fialho,Sílvia Ligório; Siqueira,Rubens Camargo; Jorge,Rodrigo; Cunha Júnior,Armando da Silva.
The treatment of diseases affecting the posterior segment of the eye is limited by the difficulty in transporting effective doses of drugs to the vitreous, retina, and choroid. Topically applied drugs are poorly absorbed due to the low permeability of the external ocular tissues and tearing. The blood-retina barrier limits drug diffusion from the systemic blood to the posterior segment, thus high doses of drug are needed to maintain therapeutic levels. In addition, systemic side effects are common. Intraocular injections could be an alternative, but the fast flowing blood supply in this region, associated with rapid clearance rates, causes drug concentration to quickly fall below therapeutic levels. To obtain therapeutic levels over longer time periods,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Implants/biodegradation; Drugs/delivery systems; Drugs/prolonged delivery systems; Pharmaceutical technology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300024

Effects of purine nucleotide administration on purine nucleotide metabolism in brains of heroin-dependent rats
Provedor de dados: 60
Autores: Li,Kun; Zhang,Ping; Chen,Li; Guan,Fengying.
ABSTRACT Heroin is known to enhance catabolism and inhibit anabolism of purine nucleotides, leading to purine nucleotide deficiencies in rat brains. Here, we determined the effect of exogenous purine nucleotide administration on purine nucleotide metabolism in the brains of heroin-dependent rats. Heroin was administrated in increasing doses for 9 consecutive days to induce addiction, and the biochemical changes associated with heroin and purine nucleotide administration were compared among the treated groups. HPLC was performed to detect the absolute concentrations of purine nucleotides in the rat brain cortices. The enzymatic activities of adenosine deaminase (ADA) and xanthine oxidase (XO) in the treated rat cortices were analyzed, and qRT-PCR was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Purine nucleotide/quantitative analysis; Purine nucleotide/effects/brain rats; Heroin analysis; Adenosine deaminase analysis; Xanthine oxidase analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400787

Factors associated to antidepressant prescription for civil servants of Belo Horizonte, MG
Provedor de dados: 60
Autores: Hurtado,Renato Lopes; Magalhães,Sérgia Maria Starling; Ribeiro,Andréia Queiroz; Silveira,Micheline Rosa da.
Selecting the most suitable drug for the treatment of depression has clinical and economic implications, since both safety and cost of antidepressants (AD) vary among therapeutic subgroups. This study aimed to evaluate the prescription of antidepressants for users of the pharmacy for civil servants of PBH (FARMASERV) in 2005. Univariate, bivariate and multivariate analyzes using logistic regression were performed. Of the total prescriptions analyzed (652), the majority were for women (81.7%) and individuals aged between 19 and 59 years (81.7%). The variables independently associated with the selection of AD were gender, age and the specialty of the prescriber. Women and young adults were more likely to be prescribed an SSRI compared to their congeners,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antidepressants/rational use; Depression/treatment; Medicines/rational use.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200016

Characterization and stability studies of emulsion systems containing pumice
Provedor de dados: 60
Autores: Estanqueiro,Marilene; Conceição,Jaime; Amaral,Maria Helena; Santos,Delfim; Silva,João Baptista; Lobo,José Manuel Sousa.
Emulsions are the most common form of skin care products. However, these systems may exhibit some instability. Therefore, when developing emulsions for topical application it is interesting to verify whether they have suitable physical and mechanical characteristics and further assess their stability. The aim of this work was to study the stability of emulsion systems, which varied in the proportion of the emulsifying agent cetearyl alcohol (and) sodium lauryl sulfate (and) sodium cetearyl sulfate (LSX), the nature of the oily phase (decyl oleate, cyclomethicone or dimethicone) and the presence or absence of pumice (5% w/w). While maintaining the samples at room temperature, rheology studies, texture analysis and microscopic observation of formulations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emulsions/topical use/stability study; Emulsions/topical use/characterization; Pumice/presence in emulsions; Emulsions/texture; Emulsions/rheology; Emulsions/spreadability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200361

Tacinga inamoena vegetal drug characterization using phytochemistry, pharmacopoeial methods and thermoanalytical techniques
Provedor de dados: 60
Autores: Silva,Joanda Paolla Raimundo e; Reis,Malu Maria Lucas dos; Santana,Cleildo Pereira; Medeiros,Ana Cláudia Dantas de; Alves,Harley da Silva.
Tacinga inamoena (K. Schum.) N.P. Taylor &amp; Stuppy, also known as quipá, is a native cactus of the Caatinga used in traditional medicine to treat urethral infections and inflammation. This study aimed to determine the physicochemical characteristics of vegetal drug obtained from the roots of T. inamoena. Analytical techniques and phytochemical tests were used, such as thermal analysis, qualitative and semiquantitative determination of secondary metabolites and spectroscopy at the infrared region. The powder of the vegetal drug met the parameters established by the Brazilian Pharmacopoeia, except for compressibility, which was low. On the thermogravimetric curve, three events related to the mass loss were verified, which correlate with the vegetal drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactaceae; Quality control; Herbal medicine; Secondary metabolites; Thermogravimetry.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100602

Twenty-six years of HIV science: an overview of anti-HIV drugs metabolism
Provedor de dados: 60
Autores: Andrade,Carolina Horta; Freitas,Lenis Medeiros de; Oliveira,Valéria de.
From the identification of HIV as the agent causing AIDS, to the development of effective antiretroviral drugs, the scientific achievements in HIV research over the past twenty-six years have been formidable. Currently, there are twenty-five anti-HIV compounds which have been formally approved for clinical use in the treatment of AIDS. These compounds fall into six categories: nucleoside reverse transcriptase inhibitors (NRTIs), nucleotide reverse transcriptase inhibitors (NtRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors (PIs), cell entry inhibitors or fusion inhibitors (FIs), co-receptor inhibitors (CRIs), and integrase inhibitors (INIs). Metabolism by the host organism is one of the most important determinants of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIDS/treatment; Drugs/anti-HIV; Drugs/metabolism; Antiretroviral drugs; Biotransformation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200003

Pituitary Adenylate Cyclase Activating Peptide (1-38) and its analog (Acetyl-[Ala15, Ala20] PACAP 38-polyamide) reverse methacholine airway hyperresponsiveness in rats
Provedor de dados: 60
Autores: Tlili,Mounira; Rouatbi,Sonia; Gandia,Fedoua; Hallegue,Dorsaf; Sriha,Badreddine; Yacoubi,Mohamed Taher; Krichah,Raja; Sakly,Mohsen; Rhouma,Khémais Ben; Vaudry,David; Wurtz,Olivier; Tebourbi,Olfa.
The aim of this study was to investigate both functionally and structurally bronchodilator effects of Pituitary adenylate cyclase activating peptide (PACAP38) and acetyl-[Ala15, Ala20] PACAP38-polyamide, a potent PACAP38 analog, in rats challenged by methacholine (MeCh). Male Wistar rats were divided randomly into five groups. Groups 1 and 2 inhaled respectively aerosols of saline or increasing doses of MeCh (0.5, 1, 2.12, 4.25, 8.5, 17, 34 and 68mg/L). The other groups received terbutaline (Terb) (250 µg/rat) (10-6 M), PACAP38 (50 µg/rat) (0.1 mM) or PACAP38 analog (50 µg/rat) associated to MeCh from the dose of 4.25 mg/L. Total lung resistances (RL) were recorded before and 2 min after MeCh administration by pneumomultitest equipment. MeCh administration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary adenylate cyclase activating peptide/effects/inflammatory lung diseases; Pituitary adenylate cyclase/analogs/effects in inflammatory lung diseases; Methacholine; Bronchodilators/effects; Inflammatory lung diseases/treatment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300681

Antifungal activity of some Sternbergia taxa: effects on germ tube and biofilm formation
Provedor de dados: 60
Autores: Kaskatepe,Banu; Yilmaz,Betul Sever; Acikara,Ozlem Bahadir; Iscan,Gulcin Saltan; Vlainic,Josipa; Kosalec,Ivan.
Natural products are rapidly becoming the primary sources of novel antimicrobial agents, as resistance to existing antimicrobial agents is increasing. Apart from determining the antimicrobial activity of natural products, it is also important to understand their effects on the virulence factors of microorganisms. This study aimed to determine the antimicrobial activity of Sternbergia species prevalent in Turkey and investigate their role in the inhibition of germination tube and biofilm formation, both of which are known to be important virulence factors of Candida albicans. The antimicrobial activities of the plant extracts were evaluated using bore-plate and broth microdilution method. The extracts’ capacity to inhibit the formation of the germ-tube was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antifungal activity; Biofilm inhibition; Candida albicans; Germ tube formation; Virulence factor; Sternbergia spp.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100546

A novel approach in mucoadhesive drug delivery system to improve zidovudine intestinal permeability
Provedor de dados: 60
Autores: Pedreiro,Liliane Neves; Cury,Beatriz Stringhetti Ferreira; Chaud,Marco Vinícius; Gremião,Maria Palmira Daflon.
ABSTRACT Zidovudine (AZT) mucoadhesive solid dispersions (SD) were prepared using a sodium starch glycolate (SSG) and hypromellose phthalate (HPMCP) mixtures as carrier to enhance the intestinal permeability and bioavailability of zidovudine. SDs were prepared using the co-precipitation method followed by solvent evaporation and characterized according to their physicochemical properties such as particle size, crystallinity, thermal behavior, and liquid uptake ability. In vitro drug dissolution, mucoadhesiveness and AZT intestinal permeability were also determined. Thermal behavior and X-ray diffraction patterns demonstrated the amorphous state of AZT in SD systems. The HPMCP polymer restricted the liquid uptake ability in the acid medium; however, this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zidovudine; Solid dispersion; Mucoadhesion; P-glycoprotein; Intestinal permeability.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400715

Potential interactions of central nervous system drugs used in the elderly population
Provedor de dados: 60
Autores: Morrone,Fernanda Bueno; Schroeter,Guilherme; Petitembert,Alessandra P.; Faggiani,Fabiana T.; De Carli,Geraldo Atillio.
OBJECTIVE: To describe the use of CNS drugs and to identify the most frequently observed potential drug interactions in the elderly living in Southern Brazil. METHODS: A population-based, transversal and observational study was carried out during 2006-2007. Four hundred and eighty elderly individuals of both genders were randomly recruited and interviewed. A validated pharmacotherapeutic questionnaire and the Micromedex® Healthcare Series were utilized to analyze potential drug interactions. A severity rating scale employing the categories of "mild", "moderate" and "severe" was used to describe the interactions. RESULTS: A population of elderly living in Southern Brazil was interviewed and 98 reported using CNS drugs, 74.5% female and 25.5% male. Out of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly; Drug-Drug interactions; Central nervous system; Tobacco; Ethanol.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200006

Presence of p-synephrine in teas commercialized in Porto Alegre (RS/Brazil)
Provedor de dados: 60
Autores: Arbo,Marcelo Dutra; Braun,Paulini; Leal,Mirna Bainy; Larentis,Elisa Raup; Aboy,Ana Lucia; Bulcão,Rachel Picada; Garcia,Solange Cristina; Limberger,Renata Pereira.
Citrus aurantium (bitter orange) is characterized by the presence of p-synephrine, an amine structurally and pharmacologically related to ephedrine. Besides the same adverse effects as ephedrine, nowadays it is believed that altered levels of p-synephrine can be associated to the occurrence of migraine and cluster headaches. Leaves and fruits of this species are highly commercialized in form of teas and herbal preparations, but without taking into account the risks associated with its use. This work describes a survey of teas and herbal preparations containing C. aurantium, commercialized in Porto Alegre (RS/Brazil), in order to verify the presence of p-synephrine. Comparing with the mean amount available in the supermarkets, around 20% of the teas and 10%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus aurantium; P-synephrine/adverse effects; Bitter orange; Teas/analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200012

Access and use of medicines by elderly individuals with dementia
Provedor de dados: 60
Autores: Barros,Débora Santos Lula; Silva,Dayde Lane Mendonça da; Leite,Silvana Nair.
The objective is to reveal the difficulties concerning the access and use of medicines by elderly individuals with dementia, reported by their caregivers. This qualitative study applied the participant observation method during pharmaceutical appointments performed in a specialized geriatrics service of the University Hospital of Brasília. Caregivers reported facing difficulties regarding the itinerary for medicines access in public pharmacies, as well as the high cost of these technologies in private establishments. Psychiatric symptoms, cognitive deficits, behavioral changes, apraxia, dysphagia, among other clinical manifestations of dementia syndromes, incapacitates the elderly for self-responsibility concerningthe use of drugs, which accentuates the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly/dementia/care; Caregiver; Drugs/access; Rational use of medicines; Pharmaceutical services.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100528

Application of redox reactions for the determination of valganciclovir hydrochloride in pharmaceuticals
Provedor de dados: 60
Autores: Qarah,Nagib Abdullah Salem; Basavaiah,Kanakapura; Abdulrahman,Sameer Abdulaziz Mohammed.
Two simple, selective and sensitive spectrophotometric methods were developed and validated for the determination of valganciclovir hydrochloride (VLGH) in pure drug and tablets. The first method was based on the reduction of iron(III) to iron(II) by VLGH and subsequent formation of iron(III)-ferricyanide complex (Prussian blue) in acid medium which was measured at 730 nm (method A). In the second method (method B), permanganate was reduced by VLGH to bluish green manganate in alkaline medium and the absorbance was measured at 610 nm. The absorbance measured in each case was related to VLGH concentration. The experimental conditions were carefully studied and optimized. Beer’s law was obeyed over the concentration ranges of 2.5-20.0 and 2.0-40.0 µg mL-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Valganciclovir hydrochloride/determination; Spectrophotometry/method validation; Pharmaceutical formulations.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100516

Formulation and evaluation of bi-layer floating tablets of ziprasidone HCl and trihexyphenidyl HCl
Provedor de dados: 60
Autores: Dinakaran,Sathis Kumar; Kumar,Santhos; Banji,David; Avasarala,Harani; Rao,Venkateshwar.
The purpose of this research study was to establish ziprasidone HCl NR 40 mg and trihexyphenidyl HCl SR 4mg in the form of bi-layer sustained release floating tablets. The tablets were prepared using sodium HPMC K4M / HPMC K15M as bio-adhesive polymers and sodium bicarbonate acting as a floating layer. Tablets were evaluated based on different parameters such as thickness, hardness, friability, weight variation, in vitro dissolution studies, content of active ingredient and IR studies. The physico-chemical properties of the finished product complied with the specifications. In vitro release from the formulation was studied as per the USP XXIII dissolution procedure. The formulations gave a normal release effect followed by sustained release for 12 h which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bi-layer tablet; Ziprasidone HCl; Trihexyphenidyl HCl; Sustained release.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300012

Content design and validation of a Standard Operating Procedure to provide pharmacotherapy follow-up for the elderly in Cuba
Provedor de dados: 60
Autores: Ortega López,Irma Leonor; Dupotey Varela,Niurka María; Reyes Hernández,Ivette; Verdecia Rosés,Miguel Ernesto; Veranes Vera,Yoenia; Sagaró Yi,Natacha de la Concepción; Núñez Pérez,Claudia Lisset; Barroso Barrientos,Arlettys.
ABSTRACT Incorporating methodological tools that allow uniform and standardized development from the clinical pharmacy services to the Pharmaceutical Care practice, is nowadays a necessity. Considering the importance of pharmaceutical care provision to elderly patients, this manuscript introduces the design and content validation of a standard operating procedure to provide pharmacotherapy follow-up to the elderly in nursing homes in Cuba. The procedure was designed based on a deep analysis of documents relative to experiences focused on holistic care to elderly patients, criteria for the identification and assessment of potentially inappropriate prescriptions and the relationship between functional geriatric evaluation and the use of drugs. The content...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Standardized Operating Procedure/validation; Pharmacotherapy Follow-Up/elderly/Cuba; Pharmaceutical Care/Cuba; Comprehensive Health Carefor/elderly; Delphi methodology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200620

Use of hydrophilic and hydrophobic polymers for the development of controlled release tizanidine matrix tablets
Provedor de dados: 60
Autores: Ali,Tariq; Shoaib,Muhammad Harris; Yousuf,Rabia Ismail; Jabeen,Sabahat; Muhammad,Iyad Naeem; Tariq,Asfia.
The aim of the present study was to develop tizanidine controlled release matrix. Formulations were designed using central composite method with the help of design expert version 7.0 software. Avicel pH 101 in the range of 14-50% was used as a filler, while HPMC K4M and K100M in the range of 25-55%, Ethylcellulose 10 ST and 10FP in the range of 15 - 45% and Kollidon SR in the range of 25-60% were used as controlled release agents in designing different formulations. Various physical parameters including powder flow for blends and weight variation, thickness, hardness, friability, disintegration time and in-vitro release were tested for tablets. Assay of tablets were also performed as specified in USP 35 NF 32. Physical parameters of both powder blend and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tizanidine/controlled release; Hydroxypropyl methylcellulose/controlled release agent; Ethylcellulose/controlled release agent; Kollidon SR/controlled release agent; Tablets/controlled release.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400799

Antibacterial activity of propolis-based toothpastes for endodontic treatment
Provedor de dados: 60
Autores: Victorino,Fausto Rodrigo; Bramante,Clovis Monteiro; Watanabe,Evandro; Ito,Izabel Yoko; Franco,Selma Luci; Hidalgo,Mirian Marubayashi.
This study evaluated the antibacterial activity of propolis-based toothpastes used as intracanal medication in endodontic treatment. The propolis-based toothpastes were prepared using an extract established in previous studies (identified as A70D and D70D). Calcium hydroxide paste was used as a control. The bacteria employed were Streptococcus mutans (ATCC 25175), Staphylococcus aureus (ATCC 6538), Staphylococcus aureus (ATCC 25923), Kocuria rhizophila (ATCC 9341), Escherichia coli (ATCC 10538), Pseudomonas aeruginosa (ATCC 27853), Enterococcus hirae (ATCC 10541), Streptococcus mutans (ATCC 25175). Five field strains isolated from saliva were used: Staphylococcus spp. (23.1 - coagulase positive), Staphylococcus spp. (23.5 - coagulase negative),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis/antibacterial activity; Tooth paste/with propolis/antibacterial activity; Endodontics/treatment.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400025

Development and evaluation of ofloxacin orally disintegrating tablets
Provedor de dados: 60
Autores: Mohite,Badrinath Pralhadrao; Chafle,Sandip Annaji; Somasundaram,Jayaprakash; Avari,Jasmine Gev.
Bitter taste of ofloxacin, a broad spectrum bactericidal agent, is masked and orally disintegrating tablets were formulated. The bitter taste is masked by forming complex between drug and weak cation exchange resins, Tulsion 335 and Indion 204. Effect of pH and drug:resin ratio on the drug loading was studied. Maximum drug loading was observed at pH 6. Ratio of 1:2 of drug:resin masked almost complete bitterness of ofloxacin. Formation of complexes was confirmed by IR spectroscopy. Physical characterization of taste masked complexes was carried out. Present work envisages the taste masking of ofloxacin and development of orally disintegrating tablets. The effect of pH and resin quantities on drug loading were studied to find the optimum conditions of drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dispersion time; Indion 204; Ion exchange resin; Ofloxacin; Orally disintegrating tablets; Tableting properties; Tulsion 335; Wetting time.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200005

Theoretically nanoscale study on ionization of muscimol nano drug in aqueous solution
Provedor de dados: 60
Autores: Kiani,Farhoush; Abbaszadeh,Mehran; Pousti,Mohammad; Koohyar,Fardad.
In the present work, acid dissociation constant (pKa) values of muscimol derivatives were calculated using the Density Functional Theory (DFT) method. In this regard, free energy values of neutral, protonated and deprotonated species of muscimol were calculated in water at the B3LYP/6-31G(d) basis sets. The hydrogen bond formation of all species had been analyzed using the Tomasi's method. It was revealed that the theoretically calculated pKa values were in a good agreement with the existing experimental pKa values, which were determined from capillary electrophoresis, potentiometric titration and UV-visible spectrophotometric measurements.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanodrug; Muscimol; Acid/dissociation constants; Computational chemistry; Density Functional Theory.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100213

Simultaneous determination of amoxicillin and clavulanic acid in pharmaceutical preparations by capillary zone electrophoresis
Provedor de dados: 60
Autores: Hancu,Gabriel; Neacşu,Anamaria; Papp,Lajos Attila; Ciurba,Adriana.
ABSTRACT Clavulanic acid enhances the antibacterial spectrum of amoxicillin by rendering most β-lactamase producing isolates susceptible to the drug. A fast, simple and efficient capillary electrophoresis method was developed for the simultaneous determination of amoxicillin and clavulanic acid from complex mixtures. Using a 25 mM sodium tetraborate as background electrolyte at a pH of 9.30, + 25 kV applied voltage, 25 °C system temperature, UV determination at 230 nm; we succeeded in simultaneous separation of amoxicillin and clavulanic acid in approximately 2 minutes. The analytical performance of the method was evaluated in terms of reproducibility, precision, accuracy, and linearity. The optimized analytical method was applied for the determination of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amoxicillin/determination; Clavulanic acid/determination; Capillary electrophoresis/quantitative analysis; Antibacterials/quantitative analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200281

Development and in vitro/in vivo evaluation of thermo-sensitive in situ gelling systems for ocular allergy
Provedor de dados: 60
Autores: Güven,Umay Merve; Berkman,Murat Sami; Şenel,Behiye; Yazan,Yasemin.
Ocular allergy is one of the most common disorders of the eye surface. Following diagnosis this condition is typically treated with preparations containing antihistamines. However, anatomy of the eye and its natural protective mechanisms create challenges for ocular drug delivery. Rapid elimination of antihistamine substances due to short residency times following application can lead to insufficient treatment of ocular allergies. With this in mind, the aim of this study was to prepare a controlled ocular delivery system to extend the retention time of olopatadine hydrochloride (OLO) and in doing so to reduce the need for frequent application. We developed extended-release ocular in situ gelling systems for which in vivo retention times were determined in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olopatadine hydrochloride; Ocular allergy; In situ gelling system.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100526

Toxicological effects of ciprofloxacin and chlorhexidine on growth and chlorophyll a synthesis of freshwater cyanobacteria
Provedor de dados: 60
Autores: Azevedo,Fernanda Cristina Rezende; Vaz,Izabela Cristina Dias; Barbosa,Francisco Antônio Rodrigues; Magalhães,Sérgia Maria Starling.
Cyanobacteria are phytoplanktonic microorganisms that are susceptible to the deleterious effects of pharmaceutical residues in the aquatic environment, which poses a challenge to the environment exposed to diverse pharmaceutical products and their potential effects. The objective of this study was to evaluate the effects of the antibiotic substances ciprofloxacin and chlorhexidine in pharmaceutical preparations on the growth and production of chlorophyll of two cyanobacterial strains, Microcystis aeruginosa and Microcystis panniformis, isolated from a lake in a Brazilian environmental protection area. The EC50 and EC10 of chlorhexidine for M. aeruginosa were 206.4 µg/L and 108.5 µg/L, respectively, and for M. panniformis were 171.4 µg/L and 116.6 µg/L,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecotoxicological test; Antibiotics; Antiseptic; Contaminants of emerging interest; Growth inhibition.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100588

Stability of furosemide and aminophylline in parenteral solutions
Provedor de dados: 60
Autores: Santos,Carolina Alves dos; Mazzola,Priscila Gava; Polacwiecz,Bronislaw; Knirsch,Marcos Camargo; Cholewa,Olívia; Penna,Thereza Christina Vessoni.
Parenteral solutions (PS) are one of the most commonly used drug delivery vehicles. Interactions among the drug, components in the drug's formulation, and the PS can result in the formation of inactive complexes that limit efficacy or increase side effects. The aim of this work was to evaluate possible interactions between the drugs and PS, assess drug stability and to identify degradation products after 20 h at room temperature. Furosemide (FSM) and Aminophylline (APN) were added to PS containing either 20% mannitol or 0.9% NaCl at pH 6.5-7.5 and 10-11. Their behavior was studied individually and as an admixture, after 1 h oxidation with H2O2, using a spectrophotometer and HPLC. Individually, FSM and APN added to 20% mannitol and 0.9% NaCl solutions had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parenteral solutions; Spectrophotometer; High Performance Liquid Chromatography; Furosemide; Aminophylline.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100011

Production of vegetable oil blends and structured lipids and their effect on wound healing
Provedor de dados: 60
Autores: Ract,Juliana Neves Rodrigues; Soares,Fabiana Andreia Schäfer De Martini; Rodrigues,Hosana Gomes; Bortolon,José Ricardo; Murata,Gilson Masahiro; Gonçalves,Maria Inês Almeida; Hatanaka,Elaine; Curi,Rui; Gioielli,Luiz Antonio.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Structured lipid/effect/wound healing; Vegetable oil/effect/wound healing; Healing; Cutaneous wound healing/treatment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200415

Statistical optimization of dithranol-loaded solid lipid nanoparticles using factorial design
Provedor de dados: 60
Autores: Gambhire,Makarand Suresh; Bhalekar,Mangesh Ramesh; Gambhire,Vaishali Makarand.
This study describes a 3² full factorial experimental design to optimize the formulation of dithranol (DTH) loaded solid lipid nanoparticles (SLN) by the pre-emulsion ultrasonication method. The variables drug: lipid ratio and sonication time were studied at three levels and arranged in a 3² factorial design to study the influence on the response variables particle size and % entrapment efficiency (%EE). From the statistical analysis of data polynomial equations were generated. The particle size and %EE for the 9 batches (R1 to R9) showed a wide variation of 219-348 nm and 51.33- 71.80 %, respectively. The physical characteristics of DTH-loaded SLN were evaluated using a particle size analyzer, differential scanning calorimetry and X-ray diffraction. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid lipid nanoparticles; Homogenization; Ultrasonication; 3² factorial design; Dithranol.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300008

Simultaneous determination of clobutinol hydrochloride and doxylamine succinate from syrups by RP HPLC using a new stationary phase containing embedded urea polar groups
Provedor de dados: 60
Autores: Rosa,Paulo Cesar Pires; Jardim,Isabel Cristina Sales Fontes.
A new, simple, fast, reproducible and sensitive reversed phase HPLC method, using a new stationary phase containing embedded urea polar groups, has been developed and validated for the simultaneous determination of clobutinol hydrochloride (CLO) and doxylamine succinate (DOX) in syrups. The determination was carried out on a C8 urea column (125 mm x 3.9 mm i.d., 5 µm particle size) synthetized at the Liquid Chomatography Laboratory (LabCrom) of the Chemistry Institute of Unicamp. The mobile phase consisted of a mixture of acetonitrile:methanol:phosphate buffer (pH 2.5) in the gradient mode. The diode array detector (DAD) was operated at 230 nm for CLO and 262 nm for DOX. The method showed adequate precision, with relative standard deviations (RSD) less...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clobutinol hydrochloride/determination; Doxylamine succinate/determination; High performance liquid chromatogralhy/quantitative analysis; Cloridrato de clobutinol/determinação; Succinato de doxilamina/determinação; Cromatografia líquida de alta eficiência/análise quantitativa.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200015

Pharmacokinetic study with computational tools in the medicinal chemistry course
Provedor de dados: 60
Autores: Brito,Monique Araújo de.
To improve the teaching-learning process in the Medicinal Chemistry course, new strategies have been incorporated into practical classes of this fundamental discipline of the pharmaceutical curriculum. Many changes and improvements have been made in the area of medicinal chemistry so far, and students should be prepared for these new approaches with the use of technological resources in this field. Practical activities using computational techniques have been directed to the evaluation of chemical and physicochemical properties that affect the pharmacokinetics of drugs. Their objectives were to allow students to know these tools, to learn how to access them, to search for the structures of drugs and to analyze results. To the best of our knowledge, this is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal chemistry/teaching-learning process; Pharmacy/education; Cheminformatics; Pharmacokinetics/teaching; Drugs/molecular properties; Drugs/teaching.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400017

Synadenium umbellatum Pax. promotes cell cycle arrest and induces apoptosis in K-562 leukemia cells
Provedor de dados: 60
Autores: Mota,Mariana Flavia da; Benfica,Polyana Lopes; Valadares,Marize Campos.
Chronic myeloid leukemia (CML) is a clonal myeloproliferative disease that shows apoptosis resistance. The introduction of imatinib mesylate has revolutionized the treatment of CML, but imatinib resistance may develop at any time and inevitably leads to disease progression. Synadenium umbellatum Pax. belongs to the Euphorbiaceae family and is popularly used in Brazil for the treatment of cancer. The cytotoxicity of Euphorbiaceae is associated with the ability of these plants and their bioactive compounds to induce apoptotic tumor cell death. Therefore, we aimed to investigate the cytotoxicity and the mechanisms of death induced by S. umbellatum extract in leukemic cells. S. umbellatum cytotoxicity was evaluated by trypan blue exclusion assay and flow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synadenium umbellatum/pharmacognosy; Synadenium umbellatum/cytotoxicity; Synadenium umbellatum/evaluation; K-562 cells/apoptosis; K-562 cells/cycle arrest.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300017

XII National Meeting of Pharmaceutical Chemistry Professors: could we have now a subarea of Pharmaceutical and Medicinal Chemistry in the CNPq Pharmacy Comittee?
Provedor de dados: 60
Autores: Ferreira,Elizabeth Igne; Barreiro,Eliezer L.; Andrade,Carolina Horta.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100001

Star anise extracts modulation of reproductive parameters, fertility potential and DNA fragmentation induced by growth promoter Equigan in rat testes
Provedor de dados: 60
Autores: Elmasry,Thanaa Ahmed; Al-Shaalan,Nora Hamed; Tousson,Ehab; El-Morshedy,Kadreya; Al-Ghadeer,Areej.
ABSTRACT Equigan is an anabolic steroid that has been developed for veterinary use and derived from endogenous sex hormone testosterone that plays a key role in the development of male reproductive tissue as well as in puberty and spermatogenesis. The current study is aimed to investigate the possible prophylactic effect of star anise extracts (SAE) on the toxicity of rat testes, sexual hormones alternations, sperm count, sperm abnormalities and testicular DNA damage by Equigan. Forty adult male rats were equally divided into four groups (1st Control group, 2nd SAE group, 3rd Equigan and 4th Equigan+SAE group). Food and fluid intakes, relative body weight, potassium, chloride, phosphorous, non-progressive and immotile sperms were significantly increased in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anabolic steroid/Equigan Testis/secretion Spermatogenesis/drug effects Sperm Marphometery Anabolic Agents/pharmacology Star anises/drug effects Illicium verum/drug effects Reproductive Control Agents/pharmacology Fertility Agents; Male/analysis DNA Fragmentation/drug effects.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100621

Brazilian Association of Pharmaceutical Sciences
Provedor de dados: 60
Autores: Lopes,João Luis Callegari.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400001

Evaluation of immunomodulatory effects of lomefloxacin in mice
Provedor de dados: 60
Autores: Majeed,Arfa; Javeed,Aqeel; Omer,Muhammad Ovais; Mushtaq,Muhammad Hassan; Sattar,Adeel.
Lomefloxacin is a flouroquinolone antibiotic that is quite efficacious against many gram negative and gram positive pathogens. Lomefloxacin evince antibacterial effects by modifying DNA gyrase in gram negative pathogens and topoisomerase IV in gram positive pathogens. This study is designed to assess the immunomodulatory effects of lomefloxacin in male albino mice. Three doses of lomefloxacin 12.5 mg/kg, 25 mg/kg and 50 mg/kg were used and delayed type hypersensitivity assay, cyclophosphamide induced neutropenic assay, carbon clearance assay, heamagglutination assay and mice lethality test were performed to evaluate the effects of lomefloxacin on immune system of mice. DTH assay has depicted the significant immunosuppressant potential of lomefloxacin at 25...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell-mediated; Humoral; Immunomodulatory; Immune response; Lomefloxacin.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100628

Optimization of self-nanoemulsifying formulations for weakly basic lipophilic drugs: role of acidification and experimental design
Provedor de dados: 60
Autores: Shahba,Ahmad Abdul-Wahhab; Mohsin,Kazi; Alanazi,Fars Kaed; Abdel-Rahman,Sayed Ibrahim.
ABSTRACT Formulators face great challenges in adopting systematic approaches for designing self-nanoemulsifying formulations (SNEFs) for different drug categories. In this study, we aimed to build-up an advanced SNEF development framework for weakly basic lipophilic drugs, such as cinnarizine (CN). First, the influence of formulation acidification on CN solubility was investigated. Second, formulation self-emulsification in media with different pH was assessed. Experimentally designed phase diagrams were also utilized for advanced optimization of CN-SNEF. Finally, the optimized formulation was examined using cross polarizing light microscopy for the presence of liquid crystals. CN solubility was significantly enhanced upon external and internal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Self-nanoemulsifying formulations/solubility; Cinnarizine/lipophilic drugs; Acidification/experimental design; Solubility enhancement.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400653

Seventy years of evolution
Provedor de dados: 60
Autores: Mancini Filho,Jorge.
Tipo: Info:eu-repo/semantics/other
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300001

Development and evaluation of bilayer tablets of combination of antibiotics for the treatment of sexually transmitted disease
Provedor de dados: 60
Autores: Gaikwad,Swati Sanjay; Chafle,Sandip Annaji; Morris,Princy Sunil; Avari,Jasmine Gev.
ABSTRACT The present research work was envisaged to develop bilayer tablets to improve therapeutic efficacy of antibiotic combination for the treatment of sexually transmitted diseases. The combination of two antibiotics i.e. cefixime trihydrate and ofloxacin were used for the preparation of bilayer tablets which act against genito-urinary infections. The formulations comprise of cefixime trihydrate as immediate release layer formulated using different superdisintegrants and ofloxacin as extended release layer containing HPMC K100M. Evaluation of bilayer tablets were performed for the immediate release cefixime layer and sustain release ofloxacin layer with optimization of excipients. The immediate release layer of cefixime showed complete release within...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sexually transmitted diseases/treatment; Bilayer tablets; Cefixime trihydrate; Ofloxacin.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300555

Indexes vs. quality: considerations on how we analyze our publications and Science in Brazil
Provedor de dados: 60
Autores: Aranha,Leila Rangel de Carvalho.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200001

Studies on the formation and forming mechanism of the related substance E in potassium clavulanate production by HPLC-MS/MS
Provedor de dados: 60
Autores: Zhong,Chuanqing; Cao,Guangxiang; Jin,Xiaoqing; Wang,Fengshan.
The objective of this study was to investigate the formation and forming mechanism of the related substance E in potassium clavulanate production. The impurity with retention time of 11.1 min in potassium clavulanate final product was confirmed as the related substance E by high performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS).The related substance E analysis during the production of clavulanic acid showed that this impurity could be formed during both the fermentation and purification processes, especially in the later fermentation stage, filtration concentration and back-extraction procedure. Clavulanic acid was the precursor of the related substance E. Studies on its forming mechanism showed that the related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potassium clavulanate/production; Clavulanic acid/formation of the Related substance E; Related substance E/formation; Related substance E/forming mechanism High performance liquid chromatography/biotechnological process.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200391

Biomarker responses (serum biochemistry) in pregnant female wistar rats and histopathology of their neonates exposed prenatally to pesticides
Provedor de dados: 60
Autores: Upadhyay,Jyoti; Rana,Mahendra; Bisht,Satpal Singh; Rana,Amita; Durgapal,Sumit; Juyal,Vijay.
Experiments were conducted to investigate the effects on health of pregnant female rats exposed to pesticides glyphosate and carbendazim. Glyphosate is used as herbicide and carbendazim as a fungicide; all are commercially available readily for various agricultural and domestic purposes. The hypothesis tested in this investigation is that pesticide exposure during pregnancy causes changes in biomarker responses like serum glucose level, total protein, total cholesterol, triglycerides, SGOT, SGPT, and billirubin level. Significant changes were observed in all above biomarker responses, when compared with the reference. Histopathology of skin and kidney of rat neonates showed marked damage. Degenerative changes and vacuolization with eroded capsule were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glyphosate; Carbendazim Biomarker responses Biomarkers/ analysis Pesticide exposure/histopathology Newborn/ histopathology Herbicides/ toxicity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100507

Risk assessment of the occurrence of aflatoxin and fungi in peanuts and cashew nuts
Provedor de dados: 60
Autores: Kujbida,Paula; Maia,Patrícia Penido; Araújo,Ariadne Naama de; Mendes,Leonardo Daniel; Oliveira,Mariana Lepri de; Silva-Rocha,Walicyranison Plinio; Brito,George Queiroz de; Chaves,Guilherme Maranhão; Martins,Isarita.
In the present study, the occurrence of fungi and aflatoxins (AFs) in peanut and cashew nut samples was investigated. Mycological analysis revealed the presence of fungi in 58.8% of samples, and assessment of AFs by chromatographic methods revealed that 52.9% were contaminated by AFs. AFB1 was the principal component in all AF-contaminated samples, with a mean level of 14.0, and 1.08 µg/kg in peanut and cashew nut, respectively. Eleven samples (32.4%) exceeded the total AF maximum level (4 μg/kg) and 8 samples (23.5%) exceeded the AFB1 (2 μg/kg) established by the European Commission. Our findings suggest that the incidence of AFs emphasizes the need for regular monitoring and a more stringent food safety system to control AFs at the lowest possible levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin; Peanut; Cashew nut; Fungi; Risk assessment.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100502

Phytoconstituents from Sidastrum micranthum (A. St.-Hil.) Fryxell (Malvaceae) and antimicrobial activity of pheophytin a
Provedor de dados: 60
Autores: Gomes,Roosevelt Albuquerque; Teles,Yanna Carolina Ferreira; Pereira,Fillipe de Oliveira; Rodrigues,Luis Alberto de Sousa; Lima,Edeltrudes de Oliveira; Agra,Maria de Fátima; Souza,Maria de Fátima Vanderlei de.
abstract Sidastrum micranthum (A. St.-Hil.) Fryxell, a member of the Malvaceae family, is called malva preta in Brazil. As this species is commonly used to treat bronchitis, cough, and asthma, better knowledge of its chemical compounds is important. The phytochemical study of its hexane extract, using chromatographic techniques, led to isolation of six compounds: the triterpene isoarborinol, a mixture of sitosterol and stigmasterol, sitosterol-3-O-β-D-glucopyranoside, pheophytin a, and 132-hydroxy-(132-S)-pheophytin a. Structural identification of these compounds was carried out using spectroscopic methods such as IR and 1D and 2D NMR (HOMOCOSY, HMQC, HMBC, and NOESY). Compounds isolated from S. micranthum were screened for their in vitro antifungal and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malvaceae/phytochemistry; Sidastrum micranthum/phytochemistry; Sidastrum micranthum/antimicrobial activity/in vitro study; Pheophytins; Medicinal plants.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400861

Macromolecular confinement of therapeutic protein in polymeric particles for controlled release: insulin as a case study
Provedor de dados: 60
Autores: Guerreiro,Luiz Henrique; Silva,Daniel da; Girard-Dias,Wendell; Mascarenhas,Camile Moreira; Miranda,Kildare; Sola-Penna,Mauro; Ricci Júnior,Eduardo; Lima,Luís Mauricio Trambaioli da Rocha e.
ABSTRACT Sustained release systems for therapeutic proteins have been widely studied targeting to improve the action of these drugs. Molecular entrapping of proteins is particularly challenging due to their conformational instability. We have developed a micro-structured poly-epsilon-caprolactone (PCL) particle system loaded with human insulin using a simple double-emulsion w/o/w method followed by solvent evaporation method. This formulation is comprised by spheric-shaped microparticles with average size of 10 micrometers. In vitro release showed a biphasic behavior such as a rapid release with about 50% of drug delivered within 2 hours and a sustained phase for up to 48 h. The subcutaneous administration of microencapsulated insulin showed a biphasic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human insulin/study; Microparticles; Poly-ε-caprolactone; Therapeutic proteins/study/action; Proteins/molecular entrapping.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200622

Nanoemulsion containing caffeine for cellulite treatment: characterization and in vitro evaluation
Provedor de dados: 60
Autores: Freire,Thamires Batello; Dario,Michelli Ferrera; Mendes,Otávio Gil; Oliveira,Andressa Costa de; Vetore Neto,Alberto; Faria,Dalva Lucia Araújo de; Silva,Vânia Rodrigues Leite e; Baby,André Rolim; Velasco,Maria Valéria Robles.
The Ginoide Hydrolipodystrophy (GHLD), commonly known as cellulite, occurs in 80-90% of the female population after the puberty period and comes from a metabolic modification in the cutaneous adipose tissue. Caffeine has been used in topical formulations due to its lipolytic action. We studied a nanoemulsion (F3) containing caffeine with two surfactants (oleth-3 and oleth-20) by emulsification method by phase inversion temperature inversion (PIT). The polydispersion indices (PDI) showed the reduced deviation of 0.1. The mean droplet size was ~ 40 nm. The evaluated constant of Ostwald, in the refrigerator condition was the most favorable during the stability test. In the In Raman spectroscopy assay, the caffeine bands found in F3 were compatible with those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulite; Caffeine; Gynoid hydrolipodystrophy; Nanoemulsion; Permeation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100567

Passion fruit (Passiflora edulis) leaf extract modulates the oxidative metabolism of rat peritoneal neutrophils in a model of inflammation
Provedor de dados: 60
Autores: Araújo,Cleiber Lucan Alves; Salles,Bruno Cesar Correa; Duarte,Stella Maris da Silveira; Rodrigues,Maria Rita; Paula,Fernanda Borges de Araújo.
This study was conducted to evaluate the effect of extracts of Passiflora edulis Sims leaves on the oxidative metabolism of rat peritoneal neutrophils using a model of acute inflammation. The extract was obtained by maceration in 70% ethanol, evaporation under reduced pressure and lyophilisation. Total phenolic content (TP) was determined by the Folin-Ciocalteu assay. The P. edulis extract, in different doses, was administered by gavage 1 h prior to inflammation induction by carrageenan (8 mg/kg, i.p.); five hours later, the neutrophils were obtained by intraperitoneal lavage. The tests performed in neutrophils were cytochrome C and chemiluminescence assay as well as myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione peroxidase (GPx) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis Sims; Inflammation; Neutrophil; Reactive oxygen species (ROS); Antioxidant enzymes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100562

In vitro antimicrobial activity of total extracts of the leaves of Petiveria alliacea L. (Anamu)
Provedor de dados: 60
Autores: Ochoa Pacheco,Ania; Marín Morán,Jorge; González Giro,Zenia; Hidalgo Rodríguez,Adrian; Mujawimana,Rachel Juliet; Tamayo González,Karel; Sariego Frómeta,Sandra.
The antimicrobial activity of 13 total extracts was evaluated, 10 soft extracts (B) and 3 blended extracts (E) prepared from dry and fresh leaves of Petiveria alliacea L. Various solvents were used for their preparation: hydroalcoholic solution at 30%, 80% and isopropyl alcohol. The antimicrobial effect of the extracts was tested by means of the method of Kirby-Bauer, using four bacterial strains from the ATCC collection (Escherichia coli, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa) and a leveduriform fungus (Candida albicans). The following quality control parameters were determined for most active extracts: physical, physical-chemical and chemical parameters. The results were: nine extracts showed antibacterial activity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial activity; Anamu; Soft extracts; Blended extracts; Minimum Inhibitory Concentration.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200006

Food and nutrition
Provedor de dados: 60
Autores: Menezes,Elizabete Wenzel de.
Tipo: Info:eu-repo/semantics/review
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100029

Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum
Provedor de dados: 60
Autores: Silva,Alessandra Tesch da; Lima,Ewelyne Miranda de; Caliman,Isabela Faco; Porto,Leonardo Luiz Souza; Nascimento,Andrews Marques do; Kalil,Iêda Carneiro; Lenz,Dominik; Bissoli,Nazaré Souza; Endringer,Denise Coutinho; Andrade,Tadeu Uggere de.
The aims of this study were to evaluate the chemical profile, vascular reactivity, and acute hypotensive effect (AHE) of the ethanolic extract of leaves of Alpinia purpurata (Vieill) K. Schum (EEAP). Its chemical profile was evaluated using HPLC-UV, ICP-OES, and colorimetric quantification of total flavonoids and polyphenols. The vascular reactivity of the extract was determined using the mesenteric bed isolated from WKY. AHE dose-response curves were obtained for both EEAP and inorganic material isolated from AP (IAP) in WKY and SHR animals. Cytotoxic and mutagenic safety levels were determined by the micronucleus test. Rutin-like flavonoids were quantified in the EEAP (1.8 ± 0.03%), and the total flavonoid and polyphenol ratios were 4.1 ± 1.8% and 5.1 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpinia; Alpinia purpurata/phytochemistry; Alpinia purpurata/ethanolic extract/vascular reactivity; Alpinia purpurata/ethanolic extract/acute hypotensive effect; Flavonoids/quantification; Polyphenols/quantification.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200309

Therapeutic monoclonal antibodies: scFv patents as a marker of a new class of potential biopharmaceuticals
Provedor de dados: 60
Autores: Pucca,Manuela Berto; Bertolini,Thaís Barboza; Barbosa,José Elpidio; Galina,Simone Vasconcelos Ribeiro; Porto,Geciane Silveira.
Monoclonal antibodies represent the fastest growing class of biopharmaceutical products and have a host of applications in medical research, diagnosis, therapy, and basic science. The production of recombinant monoclonal antibodies has revolutionized the generation of immunoglobulins, and their use represents a strategic breakthrough, affecting the global pharmaceutical market for therapeutic proteins. In the present work, a review of scFv, and the number of related patents, has been carried out. The results show that several countries have scFv patents, most notably the United States, China and United Kingdom. The target of these scFv antibodies was also assessed and the results demonstrate that most are directed toward cancer therapy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monoclonal antibodies; Recombinant antibodies; ScFv; Biopharmaceuticals.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100005

Analysis of the pharmaceutical assistance cycle in Romelândia, Santa Catarina, Brazil
Provedor de dados: 60
Autores: Prevedello,Patrícia; Busato,Maria Assunta.
The Pharmaceutical Assistance Cycle (PA) is the basis for healthcare services through which pharmaceutical assistance and expertise can be implemented in a functional and structured way to meet public health demands, adhere to the Brazilian Ministry of Health vision, and strike a balance in the contemporary struggle for health equity. The aim of this research is to analyze the effectiveness of the PA cycle in the city of Romelândia (SC), through a study conducted at a Primary Healthcare Unit in the municipality. For the study, 120 patients (service users) and seven healthcare professionals were interviewed. The majority of patients confirmed that they were given advice and instruction on dosage during the dispensing process, as well as advice on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical Assistance; Medicines/appropriate use; Patients/use of medicines; Medicines/use instructions.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100016

Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature
Provedor de dados: 60
Autores: Jerônimo,Heloísa Maria Ângelo; Queiroga,Rita de Cássia Ramos do Egypto; Costa,Ana Caroliny Vieira da; Barbosa,Isabella de Medeiros; Conceição,Maria Lúcia da; Souza,Evandro Leite de.
This study assessed the effect of different growth media [BHI broth, BHI broth plus glucose (10 g/100 mL) and BHI broth plus NaCl (5 g/100 mL)] and incubation temperatures (28 or 37 ºC) on the adherence, detachment and biofilm formation on polypropylene and stainless steel surfaces (2 x 2 cm coupons) for a prolonged period (24-72 h) by some strains of Staphylococcus aureus (S3, S28 and S54) from food processing plants. The efficacy of the sanitizers sodium hypochlorite (250 mg/mL) and peracetic acid (30 mg/mL) in reducing the number of viable bacterial cells in a preformed biofilm was also evaluated. S. aureus strains adhered in highest numbers in BHI broth, regardless of the type of surface or incubation temperature. Cell detachment from surfaces revealed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus/food processing; Biofilm; Surfaces; Sanitizers/efficacy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400018

Synthesis, quality control and dosimetry of the radiopharmaceutical 18F-sodium fluoride produced at the Center for Development of Nuclear Technology - CDTN
Provedor de dados: 60
Autores: Silveira,Marina Bicalho; Soares,Marcella Araugio; Valente,Eduardo Sarmento; Waquil,Samira Soares; Ferreira,Andréa Vidal; Santos,Raquel Gouvêa dos; Silva,Juliana Batista da.
18F-Sodium fluoride (Na18F) is a radiopharmaceutical used for diagnosis in nuclear medicine by positron emission tomography (PET) imaging. Bone scintigraphy is normally performed using 99mTc-MDP. However, 18F PET scans promise high quality imaging with increased resolution and improved sensitivity and specificity. In order to make available a tool for more specific studies of tumors and non-oncological diseases of bone tissue, the UPPR/CDTN team undertook the production and quality control of Na18F injectable solution with the physical-chemical, microbiological and biological characteristics recommended in the U.S. Pharmacopeia. Na18F radiochemical purity was 96.7 ± 1.3 %, with Rf= 0.026 ± 0.006. The product presented a pH of 5.3 ± 0.6, half life of 109.0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals/biodistribution; Sodium fluoride 18F/production; Sodium fluoride 18F/quality control; Sodium fluoride. 18F/dosimetry; Bone imaging.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300021

Characterization and evaluation of acid-modified starch of Dioscorea oppositifolia (Chinese yam) as a binder in chloroquine phosphate tablets
Provedor de dados: 60
Autores: Okunlola,Adenike; Akingbala,Oluwatomi.
Chinese yam (Dioscorea oppositifolia) starch modified by acid hydrolysis was characterized and compared with native starch as a binder in chloroquine phosphate tablet formulations. The physicochemical and compressional properties (using density measurements and the Heckel and Kawakita equations) of modified Chinese yam starch were determined, and its quantitative effects as a binder on the mechanical and release properties of chloroquine phosphate were analyzed using a 2³ full factorial design. The nature (X1), concentration of starch (X2) and packing fraction (X3) were taken as independent variables and the crushing strength-friability ratio (CSFR), disintegration time (DT) and dissolution time (t80) as dependent variables. Acid-modified Chinese yam...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid hydrolysis; Chinese yam/starch; Chinese yam/binding properties; Factorial design; Drugs/release; Dioscorea oppositifolia/pharmaceutics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400009

Factors associated with adherence to immunomodulator treatment in people with multiple sclerosis
Provedor de dados: 60
Autores: Câmara,Nair Assunta Antônia Corso; Gondim,Ana Paula Soares.
Abstract To determine the association between factors and adherence to immunomodulator treatment in people with multiple sclerosis treated in reference centers. Cross-sectional study conducted with 188 people who used immunomodulators in three reference centers in Ceará from March to July 2012. Adherence was assessed using the Moriskscale and factors were assessed using a questionnaire addressing socioeconomic and personal characteristics, the disease, the use of immunomodulator and educational activities. The determination of the association was expressed in crude and adjusted odds ratio with a 95% confidence interval. Adherence rate was 46% and after the logistic regression model the adherence to immunomodulator treatment was positively associated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiple sclerosis/medication adherence; Multiple sclerosis/treatment; Immunologic factors; Risk factors.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100627

Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents
Provedor de dados: 60
Autores: Mazzola,Priscila Gava; Jozala,Angela Faustino; Novaes,Letícia Célia de Lencastre; Moriel,Patricia; Penna,Thereza Christina Vessoni.
Due to the growing number of outbreaks of infection in hospital and nurseries, it becomes essential to set up a sanitation program that indicates that the appropriate chemical agent was chosen for application in the most effective way. Validating the effectiveness of decontamination and disinfection is an important and often challenging task. In order to study and compare the behavior of selected microorganisms, they were submitted to minimal inhibitory concentration (MIC). The MIC intervals, which reduced bacteria populations over 6 log10, were: 59 to 156 mg/L of quaternary ammonium compounds (QACs); 63 to 10000 mg/L of chlorhexidine; 1375 to 3250 mg/L of glutaraldehyde; 39 to 246 mg/L of formaldehyde; 43750 to 87500 mg/L of ethanol; 1250 to 6250 mg/L of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minimal inhibitory concentration; Disinfectant agents; Sterilizing agents; Sanitation/programs.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200008

5-Aminolevulinic acid photoactivated over planktonic and biofilm forms of Enterococcus faecalis as a pharmacological therapy alternative
Provedor de dados: 60
Autores: Beltran-Leal,Adriana; Muñoz-Ruiz,Abraham; Esparza-Villalpando,Vicente; Castro,Yolanda; Pozos-Guillen,Amaury; Flores,Héctor.
The purpose of the study was to evaluate the antibacterial effect of protoporphyrin IX (PpIX) generated by the exogenous administration of 5-aminolevulinic acid or δ-ALA and activated with an argon laser over a planktonic and biofilm of Enterococcus faecalis (E. faecalis) as a pharmacological therapy alternative. A planktonic strain of E. faecalis was cultured with a solution of ∂-ALA (40 µg/mL)-thioglycolate solution for 13 min, and a biofilm of E. faecalis was cultured in a δ-ALA (80 µg/mL)-thioglycolate solution for 13 min. Then, both were irradiated with an argon laser. Finally, the antibacterial effect was evaluated by counting the CFU in planktonic form, and a LIVE/DEAD viability cell test. The production and accumulation of PpIX from exogenously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Photodynamic therapy; 5-aminolevulinic acid.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100572

Chronic Hepatitis C treatment for genotype 2 or 3 in Brazil: cost effectiveness analysis of peginterferon plus ribavirin as first choice treatment
Provedor de dados: 60
Autores: Blatt,Carine Raquel; Storb,Bernd; Mühlberger,Nikolai; Farias,Mareni Rocha; Siebert,Uwe.
Brazilian Guidelines to HCV treatment (2007) recommended that the first choice treatment for patients with chronic hepatitis C (CHC) and genotype 2 or 3 is interferon alpha (IFN) plus ribavirin (RBV) for 24 weeks. The aim of this study is compare the cost and effectiveness to Hepatitis C treatment in patients with genotype 2 or 3 of peginterferon alpha (PEG) as the first choice of treatment within PEG for those that do not respond to IFN. The target population is CHC patients with genotype 2 or 3 in Brazil. The interventions are: PEG-SEC (first IFN plus RBV for 24 weeks, after, for non-responders and relapsers subsequently PEG plus RBV for 48 weeks); PEG-FIRST24 (PEG+RBV for 24 weeks). The type of the study is cost-effectiveness analysis. The data sources...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C chronic/treatment; Interferon alpha/Hepatitis C treatment; Ribavirin/Hepatitis C treatment; Peginterferon alpha/Hepatitis C treatment; Hepatitis C/ treatment/cost-effectiveness evaluation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200345

The traditional Chinese medicine formulation Ruanjian Sanjie Decoction regulates the tumor matrix and improves the anti-tumor efficacy of TP-PEG-LPs
Provedor de dados: 60
Autores: Cai,Xin-jun; Wang,Zeng; Xu,Ying-ying; Ye,Lihong; Cao,Jia-wei; Ni,Jian-jun; Wu,Ting-ting.
The Ruanjian Sanjie Decoction (RSD) is a traditional Chinese medicine (TCM) formulation consisting of Spica Prunellae, Pseudobulbus Cremastrae Seu Pleiones, Concha Ostreae and Semen Coicis, and widely used as an adjuvant in anti-cancer therapy. The aim of this study was to determine the effects of RSD on the extracellular matrix (ECM) of tumors, and on the efficacy of anti-cancer nano-formulations in a tumor-bearing mouse model. The mice were treated with triptolide encapsulated in PEG-modified liposomes (TP-PEG-LPs), either alone or in combination with RSD. The combination treatment significantly retarded tumor growth relative to the untreated controls, indicating the potent adjuvant effect of RSD in targeted anti-cancer therapy. In addition, RSD also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Traditional Chinese medicine; Ruanjian Sanjie Decoction; Tumor matrix; Anti-tumor efficacy; Nano-formulations.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100605

Factors associated with statin-related adverse muscular events in adult dyslipidemic outpatients
Provedor de dados: 60
Autores: Castro,Paula Ferreira; Ribeiro,Eliane; Dorea,Egidio Lima; Pinto,Gelba Almeida; Hirata,Rosario Dominguez Crespo.
ABSTRACT Statins are the most prescribed lowering-cholesterol drugs. They are well tolerated, however, some patients present muscular adverse symptoms. Clinical and laboratory data from 120 dyslipidemic patients prescribed with statins were obtained from January to December/2013 at a University Hospital in Sao Paulo city, Brazil, to study factors associated with statin-related adverse muscular events (AME). Pharmacotherapy and statin-related AME data (serum CK elevation and any degree of myopathy, myalgia, myositis or rhabdomyolysis) of the dyslipidemic patients were recorded. The study was approved by local Ethics Committees. Simvastatin (70%) and atorvastatin (25%) were the most prescribed statins. AME related to statin treatment were found in 17% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statins/study/effects; Statins/ muscular adverse event; Dyslipidemia; Drug intolerance; Drug interaction/pharmacotherapy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400618

Toxicology in the 21st Century
Provedor de dados: 60
Autores: Barros,Silvia Berlanga de Moraes; Maria-Engler,Silvya Stuchi.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300001

Hair straighteners: an approach based on science and consumer profile
Provedor de dados: 60
Autores: Leite,Marcella Gabarra Almeida; Garbossa,Wanessa Almeida Ciancaglio; Campos,Patricia Maria Berardo Gonçalves Maia.
Technological progress has allowed women to change their natural hair conﬁguration according to their will. This type of treatment is very popular around the world, even involving the use of prohibited chemicals, such as formaldehyde. Studies of hair characterization, straighteners and toxic evaluation are available in the literature, although few studies have evaluated the consumer profile or the current legislation of Brazil and the European Union (EU) and its influence on the consumption of hair straighteners. Previous studies from our research group have shown that hair care is essential for the quality of life and well-being of women. Within this context, the present study aimed to evaluate the profile of Brazilian hair straightener consumers, as well...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hair straighteners; Brazilian legislation; European legislation; Consumer profile; Formaldehyde; Glyoxylic acid.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300400

Association of ecstasy seizure rates with district Human Development Index in the municipality of São Paulo, Brazil, from 2000 to 2007
Provedor de dados: 60
Autores: Lapachinske,Silvio Fernandes; Moreau,Regina Lucia de Moraes.
This study aimed to analyze whether ecstasy consumption is associated with the socioeconomic status in the Municipality of São Paulo, Brazil, from 2000 to 2007. We used an official, reliable and unbiased source supplied by the Department of Narcotics of the State of São Paulo (Denarc) database and the Human Development Index of the districts (HDId) where the seizures occurred. A Spearman correlation test between the average number of ecstasy seizures per million of inhabitants with the HDId was used. There were 190 seizures (totaling 47,934 tablets) spread out in 53 of the 96 districts and 51.6% were concentrated in only 8 districts. The higher rates of ecstasy seizures were directly associated with districts with high HDId that confirmed the association...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecstasy/consumption; Illegal drugs/consumption; Illegal drugs/traffic; Illegal drugs/users; São Paulo/city/use of illegal drugs; Socioeconomic status/use of illegal drugs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300529

Synthesis of some novel pyrimidine, thiophene, coumarin, pyridine and pyrrole derivatives and their biological evaluation as analgesic, antipyretic and anti-inflammatory agents
Provedor de dados: 60
Autores: El-Sharkawy,Karam Ahmed; AlBratty,Mohammed Mofreh; Alhazmi,Hassan Ahmad.
Pyrimidine derivative 3 was afforded through the reaction of compound (1) with 5-ureidohydantion (2). Product 3 underwent a cyclization to produce fused pyrimidine derivative 7, although the latter product 7 was synthesized through one step via the reaction of compound (1) with 5-ureidohydantion (2) using another catalyst. Compound 3 was oriented to react with cyclic ketones 8a,b in the presence of elemental sulfur, salicylaldehyde (10), aryldiazonium chlorides 12a,b and ω-bromo-4-methoxy- acetophenone (14), which afforded, fused thiophene derivatives 9a,b, coumarin derivative 11, arylhdrazono derivatives 13a,b and 4-methoxyphenyl butenyl derivative 15, respectively. The latter product 15 was reacted with either potassium cyanide (16a) or potassium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrimidine derivative; Thiophene; Coumarin; Pyridine; Pyrrole; Analgesic; Antipyretic and anti-inflammatory agents.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400603

Hepatitis C in prisoners and non-prisoners in Colatina, Espírito Santo, Brazil
Provedor de dados: 60
Autores: Falquetto,Tânia Cristina; Endringer,Denise Coutinho; Andrade,Tadeu Uggere de; Lenz,Dominik.
The aim of the present work was to compare hepatitis C prevalence, genotypes, and risk factors between prisoners and non-prisoners in the city of Colatina, Espírito Santo, Brazil. This cross-sectional study involved approximately 1,600 residents and 730 prisoners, all of whom were living in Colatina. The percentage of individuals who tested positive for anti-HCV was 0.1% (2/1,600) in the non-prisoner group and 1.0% (7/730) in the prisoner group, confirming a higher risk of hepatitis C in the latter group. The percentage of subjects who progressed to HCV-RNA negative was 11.1% (1/9), confirming the high probability of evolution to chronicity. Genotype 1 was the most predominant genotype found. Factors associated with increased risk of hepatitis C were being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C/prevalence; Hepatitis C/genotypes; Hepatitis C/risk factors; Hepatitis C/symptoms; Anti-HCV; Prison population/risks evaluation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400013

Bioavailability of two oral formulas of secnidazole in healthy volunteers
Provedor de dados: 60
Autores: Montovani,Patrícia Andréa Bertuol; Pinto,Ana Maria Pugens; Santos,Mauricio Bedim dos; Vieira,Daiane Loss; Prado,Anelise Weich do; Manfio,Josélia Larger.
Secnidazole is an antimicrobial agent used primarily in the treatment of amoebiasis. For this bioequivalence study of secnidazole, twenty-eight healthy female volunteers were enrolled in a randomized crossover study. Each volunteer was given a single oral dose of secnidazole test preparation and then the reference preparation, or vice versa, with a wash out interval of two weeks. The plasma concentrations of secnidazole were determined by HPLC, and the samples were extracted with tert-butyl-methyl-ether: dicloromethane (60:40, v/v). Secnidazole and its parent compound metronidazole were separated on a C18 column with water:acetonitrile (85:15, v/v) as the mobile phase, and monitored at 310 nm. The ratio of mean Cmax, AUC0-t and AUC0-∞ values for the test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral formulas/bioequivalence study; Secnidazole/bioavailability; Antimicrobials/bioavailability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400011

Internationalizing Brazilian Pharmaceutical Sciences
Provedor de dados: 60
Autores: Ferreira,Elizabeth Igne.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300001

In vitro evaluation of transdermal nicotine delivery systems commercially available in Brazil
Provedor de dados: 60
Autores: Ruela,André Luís Morais; Figueiredo,Eduardo Costa; Perissinato,Aline Gravinez; Lima,Ana Carolina Zogbi; Araújo,Magali Benjamim; Pereira,Gislaine Ribeiro.
The aim of this study was to develop and validate a method for evaluating the release and skin permeation from transdermal nicotine patches using the vertical diffusion cell (VDC). The VDC is an experimental apparatus employed in research, development, and the pharmaceutical field because it can simulate conditions closest to those established in clinical trials. Two transdermal nicotine delivery systems marketed in Brazil to release 14 mg over 24 hours were evaluated. Release studies were carried out using a regenerated cellulose dialysis membrane and permeation studies were carried out using excised porcine ear skin. The results indicated that nicotine release from both evaluated patches follows Higuchi's release kinetics, while skin permeation studies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nicotine/release and skin permeation; Transdermal delivery patches/evaluation; Vertical diffusion cell; Skin permeation; Transdermal formulations/nicotine delivery systems.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300020

Cost-effectiveness of insulin analogs from the perspective of the Brazilian public health system
Provedor de dados: 60
Autores: Cazarim,Maurílio de Souza; Rodrigues,João Paulo Vilela; Cruz-Cazarim,Estael Luzia Coelho da; Ayres,Lorena Rocha; Pereira,Leonardo Régis Leira.
ABSTRACT Human insulin is provided by the Brazilian Public Health System (BPHS) for the treatment of diabetes, however, legal proceedings to acquire insulin analogs have burdened the BPHS health system. The aim of this study was to perform a cost-effectiveness analysis to compare insulin analogs and human insulins. This is a pharmacoeconomic study of cost-effectiveness. The direct medical cost related to insulin extracted from the Ministry of Health drug price list was considered. The clinical results, i.e. reduction in glycated hemoglobin (HbA1c), were extracted by meta-analysis. Different scenarios were structured to measure the uncertainties regarding the costs and reduction in HbA1c. Decision tree was developed for sensitivity of Incremental Cost...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes Mellitus/treatment/cost-effectiveness/evaluation; Prolonged Action Insulin; Short Action Insulin; Brazilian Public Health System.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300616

Quantification of chlorogenic acid, rosmarinic acid, and caffeic acid contents in selected Thai medicinal plants using RP-HPLC-DAD
Provedor de dados: 60
Autores: Chaowuttikul,Chayanon; Palanuvej,Chanida; Ruangrungsi,Nijsiri.
The chlorogenic acid, rosmarinic acid, and caffeic acid contents in 100 selected plants were determined using reversed phase high performance liquid chromatography equipped with diode array detector. The optimum condition was 0.2% phosphoric acid in water (solvent A) and methanol (solvent B) as the mobile phase, which was set at 45% B for 20 minutes at a flow rate of 1.2 mL/min. The column temperature was maintained at 30 ºC and the detection wavelength was 325 nm. Among 100 selected plants, 39.64% contained all 3 compounds, 40.54% contained 2 compounds, 14.41% contained only 1 compound, and 5.41% could not detect any of the 3 compounds. The highest contents of chlorogenic acid, rosmarinic acid, and caffeic acid were found in Lonicera japonica flowering...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorogenic acid; Rosmarinic acid; Caffeic acid; RP-HPLC-DAD; Medicinal plant.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100507

A colleague, a friend, a fellow in the journeys: in honor of Professor Durval Mazzei Nogueira
Provedor de dados: 60
Autores: Barbério,José Carlos.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100001

Pharmaceutic guidance to hypertensive patients at USP University Hospital: effect on adherence to treatment
Provedor de dados: 60
Autores: Mori,Ana Luiza Pereira Moreira; Heimann,Joel Claudio; Dórea,Egídio Lima; Bernik,Márcia Martins Silveira; Storpirtis,Sílvia.
This study was carried out in the outpatient unit of the Teaching Hospital of the University of São Paulo (USP), and studied the impact of an educational program aimed at improving hypertensive patients' compliance to treatment. Seventy five (75) hypertensive patients of both sexes took part in the study which had no age or race discrimination. Participants presented no other concomitant pathology, except obesity, diabetes and dyslipidemia. Forty one patients were allocated to an experimental group (EG). Experimental patients attended lectures on the use of medication and artery hypertension (AH) and received personal pharmaceutical guidance for nine months. The control group (CG) comprised 34 patients who did not attend lectures or receive pharmaceutical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arterial hypertension/adhesion to treatment; Health Education; Pharmaceutical Care.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200023

Investigation of the parameters affecting the release of flurbiprofen from chitosan microspheres
Provedor de dados: 60
Autores: Tilkan,Müşerref Günseli Yüksel; Özdemir,Nurten.
ABSTRACT Flurbiprofen (FLB), a NSAID, widely used for preventing pain generally for arthritis or dental problems. In this study, FLB loaded chitosan microspheres were prepared by ionotropic gelation method. In this method, microspheres were formed by dropping chitosan solutions containing FLB into sodium alginate solutions including sodium tripolyphosphate (TPP). A variety of formulation parameters like drug:polymer ratio, drug concentration, polymer’s molecular weight, polymer concentration, pH and the concentration of TPP solutions, drying method and stirring time were analyzed. The dissolution studies were performed in a shaking water bath in pH 7.4 phosphate buffer saline (PBS) at 37 °C. Laser diffractometer was used for particle size analysis, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flurbiprofen; Chitosan; Tripolyphosphate; Ionotropic gelation; Microsphere.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400621

Strategic situational planning and management of pharmaceutical services and supply: The experience of a municipality in the State of São Paulo with regard to optimizing spending on medicinal lawsuits
Provedor de dados: 60
Autores: Portella,Amanda Patrícia Favaron; Mendes,Samara Jamile; Araújo,Raquel Queiroz de; Gastaldo,Brunno Carvalho; Leite,Silvana Nair; Storpirtis,Silvia.
One of the actions taken was to offer training on pharmaceuticals via the distance education model (EAD) with the course titled Pharmaceutical Services and Supply Management Course (PSSMC). PSSMC, which was based on the idea of expanded management, is guided by Strategic Situational Planning (PES) concepts, and structured around an Operational Plan (OP) that serves as a practical didactic tool. The aim of this study is to report and analyze a case study based on the implementation of the Operational Plan in a municipality in the State of São Paulo, while focusing on the judicialization of access to medicines and health products. Since judicialization is an important topic, the problem chosen for resolution with the PES was attendance at the issuance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strategic Planning; Judicialization; Pharmaceutical services.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100585

Glycemic effects of simvastatin: Where do we stand?
Provedor de dados: 60
Autores: Razali,Nor Razida; Huri,Hasniza Zaman; Ibrahim,Luqman; Vethakkan,Shireene Ratna; Abdullah,Bashar Mudhaffar.
ABSTRACT In clinical practice, simvastatin is usually used in the treatment of dyslipidemia patients and those at risk of or with established cardiovascular disease. However, previous studies have shown that simvastatin has the potential to affect glycemic parameters as it reportedly reduced insulin secretion and sensitivity. The exact mechanism by which simvastatin affects glycemia is still unknown, but previous studies have postulated the involvement of the glucose-insulin secretion mechanism. This review focuses on the effects of simvastatin, either alone or in combination with other lipid lowering agents, antidiabetics and antihypertensives, on glucose homeostasis. Some studies have reported that simvastatin might impair the levels of glucose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simvastatin/effects; Glucose; Insulin secretion; Insulin sensitivity; Diabetes; Concurrent medications.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100403

Development and evaluation of calcium alginate beads prepared by sequential and simultaneous methods
Provedor de dados: 60
Autores: Mandal,Sanchita; Kumar,S. Senthil; Krishnamoorthy,Balakrishnam; Basu,Sanat Kumar.
The objective of this study was to develop a sustained release dosage form of Trimetazidine dihydrochloride (TMZ) using a natural polymeric carrier prepared in a completely aqueous environment. TMZ was entrapped in calcium alginate beads prepared with sodium alginate by the ionotropic gelation method using calcium chloride as a crosslinking agent. The drug was incorporated either into preformed calcium alginate gel beads (sequential method) or incorporated simultaneously during the gelation stage (simultaneous method). The beads were evaluated for particle size and surface morphology using optical microscopy and SEM, respectively. Beads produced by the sequential method had higher drug entrapment. Drug entrapment in the sequential method was higher with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sodium alginate; Calcium alginate beads; Trimetazidine dihydrochloride; Ionotropic gelation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400021

Liquid crystalline systems containing Vitamin E TPGS for the controlled transdermal nicotine delivery
Provedor de dados: 60
Autores: Borgheti-Cardoso,Lívia Neves; Vicentini,Fabiana Testa Moura de Carvalho; Gratieri,Tais; Bentley,Maria Vitória Lopes Badra.
ABSTRACT Transdermal nicotine patches have been used in smoking cessation therapy, suggested for the treatment of skin disorders with eosinophilic infiltration and have been found to improve attention performance in patients with Alzheimer's disease and age-associated memory impairment. However, skin irritation with extended patch use is still a problem. The aim of this work was to develop a simple to prepare liquid crystalline system containing vitamin E TPGS that would be able to control nicotine delivery and reduce irritation and sensitization problems. The liquid crystalline phases were macroscopically characterized by visual analysis and examined microscopically under a polarized light microscope. Topical and transdermal delivery of nicotine were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nicotine/transdermal patches; Nicotine/controlled transdermal delivery; Liquid crystalline systems/development; Vitamin E TPGS; Skin irritation/control/in vitro study; Skin irritation/use of transdermal patches.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100021

Acute hypotensive and diuretic activities of Berberis vulgaris root bark aqueous extract in normal rats
Provedor de dados: 60
Autores: Ahmed,Muhammad; Azmat,Aisha; Khan,Salahuddin.
ABSTRACT The aim of this study is to investigate the effectiveness of intravenous administration of Berberis vulgaris root bark aqueous extract (BRBD) on the cardiovascular and renal functions of healthy normotensive rats. The different doses of BRBD 1, 10 and 20 mg/kg were administered intravenously (i.v) in normal rats. Blood pressure, diuretic activity and serum renal profile were analyzed. Intravenous injection of BRBD at the different doses of 1, 10 and 20 mg/kg showed a dose-dependent reduction in mean arterial blood pressure (P&lt;0.001). At different doses of 1, 10 and 20 mg/kg, the hypotensive effect remained for more than one hour. Single dose administration of BRBD at doses of 10 and 20 mg/kg caused a significant increase in urine output...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Berberis vulgaris root bark/ aqueous extract (BRBD); Mean arterial blood pressure (MABP); Diuresis; Serum renal profile test.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400301

Quantification of LSD in illicit samples by high performance liquid chromatography
Provedor de dados: 60
Autores: Marinho,Pablo Alves; Leite,Edna Maria Alvarez.
In the present study, a method using high performance liquid chromatography to quantify LSD, in blotter papers seized in Minas Gerais, was optimized and validated. Linearity, precision, recovery, limits of detection and quantification, and selectivity were the parameters used to evaluate performance. The samples were extracted with methanol:water (1: 1) in an ultra-sound bath. The linearity between 0.05 and 20.00 μg/mL (0.5 and 200.0μg of LSD/blotter) was observed with satisfactory mean intra and inter assay precision (RSDr = 4.4% and RSD R = 6.4%, respectively) and with mean recoveries of 83.4% and 84.9% to the levels of 1.00 and 20.00 μg/mL (10 and 200μg LSD/blotter). The limits of detection and quantification were 0.01 and 0.05 μg/mL, respectively (0.1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LSD/detection; Forensic chemistry; Analytical toxicology; In-house validation; High Performance Liquid Chromatography/quantitative analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400011

Evaluation of the influence of fluoroquinolone chemical structure on stability: forced degradation and in silico studies
Provedor de dados: 60
Autores: Bairros,André Valle de; Pereira,Danillo Baptista; Cordeiro,Everson Willian Fialho; Paim,Clésio Soldateli; Silva,Fabiana Ernestina Barcellos da; Malesuik,Marcelo Donadel; Paula,Fávero Reisdorfer.
ABSTRACT Fluoroquinolones are a known antibacterial class commonly used around the world. These compounds present relative stability and they may show some adverse effects according their distinct chemical structures. The chemical hydrolysis of five fluoroquinolones was studied using alkaline and photolytic degradation aiming to observe the differences in molecular reactivity. DFT/B3LYP-6.31G* was used to assist with understanding the chemical structure degradation. Gemifloxacin underwent degradation in alkaline medium. Gemifloxacin and danofloxacin showed more degradation perceptual indices in comparison with ciprofloxacin, enrofloxacin and norfloxacin in photolytic conditions. Some structural features were observed which may influence degradation, such...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoroquinolones/evaluation; Forced degradation studies; Molecular modeling.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100604

Effect of drying methods on the powder and compaction properties of microcrystalline cellulose derived from Gossypium herbaceum
Provedor de dados: 60
Autores: Nwachukwu,Nkemakolam; Ofoefule,Sabinus Ifeanyi.
The effect of drying method, a process variable, on the powder and compaction properties of microcrystalline cellulose (MCC) obtained from the partial acid hydrolysis of bleached alpha (α) cellulose content of matured linters of Gossypium herbaceum (GH) was investigated. A portion of the wet MCC obtained was fluid bed dried at 60 ± 1 ºC, inlet air of 30 m3 min-1 for 3 h (coded MCC-GossF). The second portion was lyophilized at - 45 ± 2 ºC for 6 h (coded MCC-GossL). The physicochemical, scanning electron micrographs, X ray diffraction patterns and micromeritic properties of the derived MCCs were determined using standard methods. The cohesiveness and compactibility of the powders were investigated using Kawakita model while the deformation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gossypium herbaceum; Powder; Compaction; Drying methods; Microcrystalline cellulose.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100581

Inclusion complex of amiodarone hydrochloride with cyclodextrins: preparation, characterization and dissolution rate evaluation
Provedor de dados: 60
Autores: Rubim,Alexandre Machado; Rubenick,Jaqueline Bandeira; Maurer,Marcela; Laporta,Luciane Varini; Rolim,Clarice Madalena Bueno.
ABSTRACT This study aimed to improve the water solubility of amiodarone hydrochloride (AMH) via inclusion complexes with β-cyclodextrin, methyl-β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin. Inclusion complexes were developed by physical mixture, coevaporation, spray-drying and freeze-drying. Solid state analysis was performed using X-ray powder diffraction, differential scanning calorimetry and scanning electronic microscopy. Thermodynamic studies demonstrate that the inclusion complexes of drug into different cyclodextrins were an exothermic process that occurred spontaneously. Water solubility and drug dissolution rates were significantly increased after the formation of inclusion complexes with the cyclodextrins evaluated in relation to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amiodarone HCl/evaluation; Amiodarone HCl/solubility; Cyclodextrins/inclusion complexes; Cyclodextrins /thermodynamic studies; Dissolution rate.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200607

Pullulan: an advantageous natural polysaccharide excipient to formulate tablets of alendronate-loaded microparticles
Provedor de dados: 60
Autores: Ferreira,Luana Mota; Velasquez,Aline de Arce; Schaffazick,Scheila Rezende; Cruz,Letícia.
This work reports the preparation of tablets by direct compression of sodium alendronate-loaded microparticles, using pullulan as filler. The tableting properties of pullulan were compared with those of microcrystalline cellulose and lactose. Pullulan tablets showed low variations in average weight, thickness and drug content. Moreover, these tablets exhibited a higher hardness compared to the other excipients. In vitro release studies showed that only pullulan was capable to maintain gastroresistance and release properties of microparticles, due to its ability to protect particles against damage caused by compression force. Thus, pullulan was considered an advantageous excipient to prepare tableted microparticles.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pullulan; Excipients; Microparticulate tablets; Sodium alendronate.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100027

Statistical evaluation of tablet coating processes: influence of pan design and solvent type
Provedor de dados: 60
Autores: Melo Junior,Valdomero Pereira de; Löbenberg,Raimar; Bou-Chacra,Nádia Araci.
Partially and fully perforated pan coaters are among the most relevant types of equipment currently used in the process of coating tablets. The goal of this study was to assess the performance differences among these types of equipment employing a factorial design. This statistical approach allowed the simultaneous study of the process variables and verification of interactions among them. The study included partially-perforated and fully-perforated pan coaters, aqueous and organic solvents, as well as hypromellose-based immediate-release coating. The dependent variables were process time, energy consumption, mean weight of tablets and process yield. For the tests, placebo tablets with a mean weight of 250 mg were produced, divided into eight lots of two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tablets/coating process; Fully perforated pan coater; Partially perforated pan coater.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400007

Determination of metals in medicinal plants highly consumed in Brazil
Provedor de dados: 60
Autores: Leal,Alexandre Soares; Prado,Guilherme; Gomes,Tatiana Cristina Bomfim; Sepe,Fernanda Peixoto; Dalmázio,Ilza.
In this work, samples of the medicinal plants: Boldo (Peumus boldus), Castanha da Índia (Aesculus hippocastanum), Chá Verde (Camelia sinensis), Erva Cidreira (Melissa officinalis), Espinheira Santa (Maytenus ilicifolia), Guaraná (Paullinia cupana), Maracujá (Passiflora sp.), Mulungu (Erythrina velutina), Sene (Cassia angustifolia) and Valeriana (Valeriana officinalis) were evaluated BY using the Neutron Activation Analysis technique (NAA- k0) in order to determine the levels of metals and other chemical contaminants. The results showed the presence of non essential elements to the human body. The diversity of chemical impurities found even at low concentration levels, considering the potential for chronic toxicity of these elements, reinforces the need to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutron activation analysis; Medicinal plants; Metals/determination in medicinal plants; Chemical contaminants; Plant products/quality control.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300022

Effects of Cymbopogon winterianus Jowitt ex Bor essential oil on the growth and morphogenesis of Trichophyton mentagrophytes
Provedor de dados: 60
Autores: Pereira,Fillipe de Oliveira; Wanderley,Paulo Alves; Viana,Fernando Antônio Cavalcanti; Lima,Rita Baltazar de; Sousa,Frederico Barbosa de; Santos,Sócrates Golzio dos; Lima,Edeltrudes de Oliveira.
Trichophyton mentagrophytes is a fungus causative agent of dermatophytosis, affecting humans worldwide. This has driven the search for products for the treatment of these infections. Accordingly, the aim of this study was to investigate the antifungal activity of the Cymbopogon winterianus essential oil against T. mentagrophytes. The antifungal tests consisted of antifungal screening, determination of MIC and MFC, analysis of the essential oil's effects on mycelial growth, germination of fungal spores, fungal viability, morphogenesis, cell wall (test with sorbitol) and cell membrane (cell leakage test) of T. mentagrophytes. Upon screening, the oil inhibited all strains, with zones of growth inhibition of 24-28 mm in diameter. The MIC was 312 μg/mL and CFM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichophyton mentagrophytes/mycology; Dermatophytosis Cymbopogon winterianus/essential oil; Dermatophytosis Cymbopogon winterianus/antifungal activity; Dermatophytes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100018

Identification of inappropriate prescribing in a Brazilian nursing home using STOPP/START screening tools and the Beers' Criteria
Provedor de dados: 60
Autores: Nascimento,Mariana Martins Gonzaga; Ribeiro,Andréia Queiroz; Pereira,Mariana Linhares; Soares,Adriana Cristina; Loyola Filho,Antônio Ignácio de; Dias-Junior,Carlos Alan Candido.
The objective of this study was to determine the prevalence of Potentially Inappropriate Medication (PIM) use and associated factors, as well as the prevalence of Prescribing Omissions (PO). A cross-sectional study was conducted in a philanthropic Brazilian nursing home involving 46 individuals aged 60 years or older. The following information was collected from medical records and drug prescriptions: gender, age, health conditions and drugs used in the past thirty days. PIM and PO were identified according to the Beers' Criteria and the STOPP/START screening tools. Over one third (37%) of the population used at least one PIM according to the Beers' Criteria (n=17) and 60.9% according to the STOPP tool. A significant association was found between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacoepidemiology; Pharmaceutical care; Elderly/inappropriate use of medicines; Medicines/inappropriate prescribing; Medicines/inappropriate use; Beers' Criteria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400911

Spectrophotometric determination of rosuvastatin in pharmaceutical formulations using quinalizarin
Provedor de dados: 60
Autores: Lima,Monica Força; Cassella,Ricardo Jorgensen; Pacheco,Wagner Felippe.
ABSTRACT This work presents the development of a methodology based on the formation of a charge transfer complex between quinalizarin and rosuvastatin, allowing for the spectrophotometric determination of rosuvastatin at 579 nm. The factors involved in the sensitivity of the technique were studied (nature and proportion of the solvent, reaction time, pH of aqueous phase and quinalizarin concentration). The proposed spectrophotometric procedures were validated with respect to linearity, ranges, precision, accuracy, detection and quantification limits. Calibration curves of the formed color products showed good linear relationships over the concentration range of 6-15 mg L-1. The proposed method has been successfully applied, which can be confirmed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosuvastatin/spectrophotometric determination; Rosuvastatin/charge transfer complex; Quinalizarin/charge transfer complex; Pharmaceutical formulations.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300603

Compression and evaluation of extended release matrix pellets prepared by the extrusion/spheronization process into disintegrating tablets
Provedor de dados: 60
Autores: Pai,Raveendra; Kohli,Kanchan; Shrivastava,Birendra.
In this study, a novel approach for compression of matrix pellets into disintegrating tablets has been studied in an attempt to overcome the issues pertaining to rupture of polymer coat during compression of reservoir-type pellets. Extended release matrix pellets were prepared by the extrusion/spheronization technique using commercially available aqueous dispersions of ethyl cellulose, acrylic polymers and sodium alginate at 10%, 20% and 30%w/w levels. Sertraline hydrochloride was used as the model drug and an in vitro release profile of 12 h was targeted. Tablets containing matrix pellets were prepared by the direct compression process. Acceptance Value, a pharmacopeial test, was applied to study the uniformity of drug distribution. Effect of compression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pellets/extended release; Pellets/evaluation; Pellets/obtention; Pellets/compression; Extrusion/spheronization technique.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100014

Development and in vitro evaluation of Letrozole loaded biodegradable nanoparticles for breast cancer therapy
Provedor de dados: 60
Autores: Dey,Sanjoy Kumar; Mandal,Bivash; Bhowmik,Manas; Ghosh,Lakshmi Kanta.
The objectives of our study were to prepare and evaluate a biodegradable nanoparticulate system of Letrozole (LTZ) intended for breast cancer therapy. LTZ loaded poly(lactide-co-glycolide) nanoparticles (LTZ-PLGA-NPs) were prepared by emulsion-solvent evaporation method using methylene chloride and polyvinyl alcohol. Percentage of drug (with respect to polymer) was selected as formulation variable. LTZ-PLGA-NPs were characterized by particle size, zeta potential, infrared spectra, drug entrapment efficiency and in vitro release. Sonication was done with an ultrasound pulse sonicator at 70 W, 30 kHz for 90 sec to produce stable NPs of mean size range from 64 nm to 255 nm with high entrapment efficiency (68% to 82%). Percentage of drug significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer/therapy; Letrozole; Biodegradable nanoparticles.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300025

Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry
Provedor de dados: 60
Autores: Ribeiro,Marcos Vinícius de Moura; Melo,Ingrid da Silva; Lopes,Francisco das Chagas da Costa; Moita,Graziella Ciaramella.
ABSTRACT Folic acid is a B complex water-soluble vitamin that is essential to humans, and its deficiency can cause problems including congenital malformations in the fetus as well as heart disease. Most countries affected by diseases associated with a lack of folic acid now supplement foods with the vitamin. There is therefore a need for the development of new analytical procedures able to determine folic acid present in different matrices. This work describes the development of zero order and first order derivative spectrophotometric methods for the determination of folic acid in different pharmaceutical formulations, using 0.1 mol L-1 NaOH as solvent. The methods are shown to be simple, selective, and robust. Good linearity was achieved, with correction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Folic acid/pharmaceutical formulations; Folic acid/pharmaceutical formulations/validation method; Folic acid/formulations/derivative spectrophotometry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400741

Implication of anthropometric profile and alimentary consumption on risk for diseases among school children in the 1st to 4th grades
Provedor de dados: 60
Autores: Monteiro,Vanessa; Matioli,Graciette.
This study aimed to determine the anthropometric profile and diet of 652 school children from the 1st to 4th grades of elementary education in six private schools in the city of Maringá, Paraná, Brazil. Children's weight and height were classified according to the Body Mass Index proposed by Must et al. (1991). Food consumption was evaluated by means of a questionnaire about daily food frequency. Statistical analysis was carried out using linear and logistic regression tests, with a significance level of 5%. High rates of overweight and obesity, 21.9% and 13.3% respectively, were observed, which did not show a significant relationship with total food consumption. The data obtained were: high consumption of foods rich in energy, such as sugar- and fat-rich...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alimentary habits; Obesity; Body mass index; Infant nutrition.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300007

The mechanism of drug interactions of a selected antiarrhythmic drug with metformin, in different animal models
Provedor de dados: 60
Autores: Khan,Kayamkani Abedulla; Satyanarayana,Sreemantula; Kumar,Kilari Eswar.
ABSTRACT This study was carried out to understand the influence of a selected antiarrhythmic drug on the pharmacodynamics and pharmacokinetics of an antidiabetic drug in animal models. Pharmacodynamic and pharmacokinetic responses were determined by measurements of blood glucose and serum insulin and serum metformin to drug interactions between disopyramide and metformin. Single dose and multi dose studies showed that the maximum blood glucose reductions in normal and diabetic rats were at the 6th hour, and in rabbits at the 3rd hour. Glucose-insulin homeostasis was evaluated to assess the safety and effectiveness of the combination. There was a marginal increase in the pharmacokinetic parameters of metformin with multiple dose treatments of disopyramide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disopyramide/pharmacokinetic interactions/pharmacodynamic; Metformin/ pharmacokinetic interactions/pharmacodynamic; Drug Interactions; Hypoglycaemia; Diabetes mellitus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400601

Formulation design and characterization of a non-ionic surfactant based vesicular system for the sustained delivery of a new chondroprotective agent
Provedor de dados: 60
Autores: Khan,Muhammad Imran; Madni,Asadullah; Ahmad,Saeed; Mahmood,Muhammad Ahmad; Rehman,Mubashar; Ashfaq,Muhammad.
Diacerein is used for symptomatic relief and cartilage regeneration in osteoarthritis. Due to gastrointestinal side effects, poor aqueous solubility and low bioavailability, its clinical usage has been restricted. The objective of the present study was to enhance its dissolution profile and to attain sustained release by designing a novel delivery system based on niosomes. Five niosomal formulations (F1-F5) with non-ionic surfactant (sorbitan monostearate) and cholesterol in varying ratios of 5:5, 6:4, 7:3, 8:2 and 9:1 were developed by the reverse-phase evaporation technique. The size and polydispersivity index (PDI) were found in the range of 0.608 µm to 1.010 µm and 0.409 to 0.781, respectively. Scanning electron microscopy (SEM) of the selected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondroprotective; Diacerein/dissolution profile; Diacerein/sustained release; Niosomes; Sorbitan monostearate; Reverse-phase evaporation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300607

Mechanical properties and total hydroxycinnamic derivative release of starch/glycerol/Melissa officinalis extract films
Provedor de dados: 60
Autores: Rechia,Letícia Mello; Morona,Juliana Bergman de Jesus; Zepon,Karine Modolon; Soldi,Valdir; Kanis,Luiz Alberto.
The aim of this study was to investigate the mechanical properties of starch/glycerol/Melissa officinalis, a topical drug delivery system for labial herpes treatment. Four films were prepared with different concentrations of starch, glycerol, and Melissa officinalis extract. The results revealed that increasing the glycerol concentration in the film reduced elasticity modulus and tensile strength, exhibiting a plasticizing effect. The increase in free volume resulted in increased release of hydroxycinnamic derivatives expressed as rosmarinic acid.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/delivery system; Glycerol; Melissa officinalis; Herpes/treatment; Polymeric film; Starch.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300012

Niosomes and liposomes as promising carriers for dermal delivery of Annona squamosa extract
Provedor de dados: 60
Autores: Mohamad,Ebtesam Abdelghany; Fahmy,Heba Mohamed.
The leaf extract of Annona squamosa L. has antibacterial, antidiabetic, antioxidant, and anticancerous activities. The present work aims to compare between liposomes and niosomes as carriers for A. squamosa extract to improve its transdermal bioavailability. Physical characterization for niosomes and liposomes was performed using: transmission electron microscope (TEM), scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). In addition, the encapsulation efficiency for A. squamosa in both carriers was evaluated and in-vitro drug release experiments were performed. The results proved the potential of both carriers to penetrate the outer layer of the skin (stratum corneum) which is considered as a strong barrier against the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annona squamosa; Dermal delivery; Liposomes; Niosomes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100525

Anti-inflammatory and analgesic activities of red seaweed Dichotomaria obtusata
Provedor de dados: 60
Autores: Vázquez,Ana Iris Frías; Sánchez,Carlos Manuel Dutok; Delgado,Neivys García; Alfonso,Ana María Suarez; Ortega,Yúlica Santos; Sánchez,Hiran Cabrera.
The aim of the present work was to investigate the anti-inflammatory and analgesic effects of red seaweed Dichotomaria obtusata, using classic tests in mice (ear edema induced by TPA and writhing induced by acetic acid). The qualitative chemical composition of the aqueous extract (lactones, phenols, triterpenes, steroids and reduced carbohydrates) obtained from this alga was also determined. The results showed that Dichotomaria obtusata (12.5, 25 and 50 mg/kg, ip) inhibited mouse ear edema in a dose-dependent manner. In the writhing test, aqueous extract (12.5, 25, 50 and 100 mg/kg, ip and 100, 200, 400, 800 mg/kg, po) significantly reduced abdominal writhes. In conclusion, this study demonstrated the anti-inflammatory and antinociceptive activities of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dichotomaria obtusata/anti-inflammatory activity; Dichotomaria obtusata/analgesic activity; Mouse ear oedema test/Writhing test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100014

Studies on new substituted pyridazinones: synthesis and biological evaluation
Provedor de dados: 60
Autores: Asif,Mohammad; Singh,Anita; Khan,Shah Alam; Husain,Asif.
We report herein the synthesis and pharmacological evaluation of a new series of 6-aryl-2-(imidazol-1-yl/1,2,4-triazol-1-yl)-2-methyl-4,5-dihydro-(2H)-pyridazin-3-one (3a-j) as potential anticonvulsant and antitubercular agents. The title compounds were prepared by reacting 6-aryl-4,5-dihydro-(2H)-pyridazin-3-one (2a-e) with formaldehyde and secondary cyclic amine imidazole or 1,2,4-triazole as per Mannich reaction. Anticonvulsant activity of pyridazinone derivatives was tested at 50 mg.kg-1 dose level against maximal electroshock (MES), isoniazid (INH, 250 mg.kg-1) and pentylenetetrazole (PTZ at 80 mg.kg-1) induced seizure methods. Phenytoin sodium (25 mg.kg-1) and sodium valproate (100 mg.kg-1) were used as reference drugs for comparison purpose....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Evaluation; Anticonvulsant; Antitubercular; MIC.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300601

Solid dispersions with hydrogenated castor oil increase solubility, dissolution rate and intestinal absorption of praziquantel
Provedor de dados: 60
Autores: Chaud,Marco Vinicius; Tamascia,Pollyanna; Lima,Andréa Cristina de; Paganelli,Maria Ondina; Gremião,Maria Palmira Daflon; Freitas,Osvaldo de.
The solubility behavior of drugs remains one of the most challenging aspects in formulation development. Solid Dispersion (SD) has tremendous potential for improving drug solubility. Although praziquantel (PZQ) is the first drug of choice in the treatment of schistosomiasis, its poor solubility has restricted its delivery oral route. In spite of its poor solubility, PZQ is well absorbed in the gastrointestinal tract, but large doses are required to achieve adequate concentration at the target sites. The aim of this study was to improve the solubility and dissolution rate of PZQ and to evaluate its intestinal absorption. SDs were formulated with PEG-60 castor oil hydrogenated (CR-60) using a fusion and evaporation method. Pure PZQ and physical mixtures (PM)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solid dispersion; Praziquantel/dissolution rates; Praziquantel/solubility; Praziquantel/intestinal absorption.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300010

Formulation of gastroretentive floating drug delivery system using hydrophilic polymers and its in vitro characterization
Provedor de dados: 60
Autores: Meka,Venkata Srikanth; Dharmanlingam,Senthil Rajan; Kolapalli,Venkata Ramana Murthy.
The aim of the present research is to formulate and evaluate the gastroretentive floating drug delivery system of antihypertensive drug, propranolol HCl. Gastroretentive floating tablets (GRFT) were prepared by using a synthetic hydrophilic polymer polyethylene oxide of different grades such as PEO WSR N-12 K and PEO 18 NF as release retarding polymers and calcium carbonate as gas generating agent. The GRFT were compressed by direct compression strategy and the tablets were evaluated for physico-chemical properties, in vitro buoyancy, swelling studies, in vitro dissolution studies and release mechanism studies. From the dissolution and buoyancy studies, F 9 was selected as an optimized formulation. The optimized formulation followed zero order rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroretentive floating/ drug delivery system; Gastroretentive floating tablets/in vitro dissolution; Hydrophilic polymers/use in drug delivery system; Polyethylene oxide; Propranolol HCl/delivery system.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200431

Studies on the formation and synthetic mechanism of related substance G in potassium clavulanate production
Provedor de dados: 60
Autores: Jin,Xiaoqing; Cao,Guangxiang; Zhang,Xiaoxiao; Chen,Yuguo; Wang,Liang; Zhong,Chuanqing.
The objective of this study was to investigate the formation and synthetic mechanism of related substance G in potassium clavulanate production. The impurity in the potassium clavulanate final product, with a retention time of 13.5 min, was confirmed as related substance G by high performance liquid chromatography-mass spectrometry/mass spectrometry. Related substance G analysis during the production of clavulanic acid showed that this impurity could be synthesized during fermentation, and the amount increased with the fermentation time. Studies on its synthetic mechanism showed that L-tyrosine and succinic acid were the precursors for biosynthesis of related substance G in vivo. The reaction was deduced to be catalyzed by an enzyme. The enzyme was a type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potassium clavulanate; Clavulanic acid; Related substance G; Related substance G; High Performance Liquid Chromatography-Mass Spectrometry-Mass Spectrometry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100077

Evaluation of the profile of drug therapy administered through enteral feeding tube in a general hospital in Rio de Janeiro
Provedor de dados: 60
Autores: Silva,Mario Jorge Sobreira da; Cava,Carlos Eduardo Meireles; Pedroso,Patrícia Kaiser; Futuro,Débora Omena.
Enteral nutrition (EN) is the method of choice for patients that cannot adequately receive oral feeding despite good gastrointestinal tract condition. Enteral diets may be administered through tube or ostomy placed in the stomach, duodenum or jejunum. The administration of drugs via enteral feeding tube (EFT) is a common practice in hospitals due to patient clinical status, and requires special attention from professionals involved in this process. This study entailed an analysis of the profile of drug therapy through EFT based on evaluation of medical prescriptions of the Medical Clinic of the Hospital dos Servidores do Estado (HSE) of Rio de Janeiro sent to the Pharmacy Service between January and June 2007, according to standard protocols in place....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteral nutrition; Drugs/prescriptions; Hospital pharmacy; Pharmaceutical care.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200014

Design of antiseptic formulations containing extract of Plinia cauliflora
Provedor de dados: 60
Autores: Oliveira,Lara Alexandre de; Souza-Moreira,Tatiana Maria de; Cefali,Letícia Caramori; Chiari,Bruna Galdorfini; Corrêa,Marcos Antonio; Isaac,Vera Lucia Borges; Salgado,Hérida Regina Nunes; Pietro,Rosemeire Cristina Linhari Rodrigues.
The leaves of the Brazilian species Plinia cauliflora were used to obtain active hydroalcoholic extract and fractions enabling the development of efficient antiseptic pharmaceutical formulations. A chemical composition of 70% ethanol extract, aqueous and ethyl acetate fractions was analyzed by thin-layer chromatography and for phenol content. Antimicrobial activity was tested against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus acidophilus and Candida albicans by the agar diffusion method and the minimum inhibitory concentration was assayed by broth microdilution. Extract microbiological quality was tested to avoid contamination in the formulations. A mouthwash and a topical cream containing the extract were developed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiseptic formulations/design; Plinia cauliflora/antimicrobial activity; Plinia cauliflora/antiseptic activity; Mouthwash; Plant extract; Topical cream.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300010

Aggravation of cyclophosphamide-induced reproductive toxicity in mice by aqueous extract of Aegle marmelos (L.)
Provedor de dados: 60
Autores: Singh,Sangita; Mishra,Awadhesh Kumar; Lata,Swarn; Tiwari,Kavindra Nath.
ABSTRACT Aegle marmelos (L.) (Rutaceae) commonly known as bael is an important medicinal fruit tree. The present study focused on the effects of aqueous extract of Aegle marmelos (AEAM) on the testis and sperm characteristics induced by cyclophosphamide (CPA) in mice. Thirty six adult Parke’s strain mice were divided into six groups: group I given only distilled water (control); group II administered with AEAM alone once in a week for five weeks; group III administered with CPA (200 mg/kg b.w., intraperitoneally) once in a week for five weeks and group IV-VI CPA along with AEAM (400, 500 and 600 mg/kg b.w., orally). CPA was found to reduce gonadosomatic index (GSI), sperm counts, motility, viability, antioxidant activities and induced histopathological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aegle marmelos (L.)/extract/effects/sperm; Cyclophosphamide; Gonadosomatic index (GSI); Reproductive toxicity; Spermatozoa.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300614

Diacerein, its beneficial impact on chondrocytes and notable new clinical applications
Provedor de dados: 60
Autores: Kaur,Daljit; Kaur,Jaspreet; Kamal,Sukhmeet Singh.
Diacerein (DCN) was obtained by diacetylation of an anthraquinone derivative rhein and was approved by FDA in 2008, in the treatment of osteoarthritis due to its inhibitory effect on proinflammatory cytokines, including IL-6 and IL-1β. It was synthesized in 1980s and marketed as a tablet in some European Union and Asian countries from 1994. Along with its great potential in the treatment of osteoarthritis, its other applications are also being explored day by day, such as in the treatment of psoriasis, epidermolysis bullosa, breast cancer, type 2 diabetes and periodontitis. The main aim of this review is to explore mechanism of action, various applications and side effects associated with DCN. This has been reviewed that apart from the risk of diarrhea on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diacerein/osteoarthritis; Drug/chondroprotective; Diacerein/mechanism of action; Drug/adverse effects; Diacerein/contraindications.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400201

Antidiabetic, antihyperlipidemic and antioxidant influences of the spice cinnamon ( Cinnamomum zeylanicumon ) in experimental rats
Provedor de dados: 60
Autores: Beji,Raja Serairi; Khemir,Sameh; Wannes,Wissem Aidi; Ayari,Khaoula; Ksouri,Riadh.
ABSTRACT The present study investigates the effect of cinnamon (Cinnamomum zeylanicumon) powder supplementation on glucose levels, lipid profiles, and oxidative stress parameters in alloxan-induced diabetic rats. Diabetes was induced in adult male Wistar rats via a single subcutaneous alloxan injection (15 mg/kg). Cinnamon powder was mixed with the standard feed of the rats in an amount of 5% for 28 consecutive days. Serum concentrations of total cholesterol (TC) and triglycerides (TG) were assayed at the end of the experimental period in all investigated groups. Anti-oxidative enzymes such as glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) were sought in the serum and pancreas. Alloxan caused the fasting blood sugar level to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cinnamon/extract/antidiabetic activity; Cinnamon/extract/antihyperlipidemic activity; Cinnamon/extract/antioxidant activity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200604

Guidance for nuclear medicine staff on radiopharmaceuticals drug interaction
Provedor de dados: 60
Autores: Santos-Oliveira,Ralph.
Numerous drug interactions related to radiopharmaceuticals take place every day in hospitals many of which are not reported or detected. Information concerning this kind of reaction is not abundant, and nuclear medicine staff are usually overwhelmed by this information. To better understand this type of reaction, and to help nuclear medicine staff deal with it, a review of the literature was conducted. The results show that almost all of radiopharmaceuticals marketed around the world present drug interactions with a large variety of compounds. This suggests that a logical framework to make decisions based on reviews incorporating adverse reactions must be created. The review also showed that researchers undertaking a review of literature, or even a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear medicine; Radiology; Radiopharmaceuticals/drug interaction; Molecular imaging.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400003

Development and validation of dissolution method for carvedilol compression-coated tablets
Provedor de dados: 60
Autores: Shah,Ritesh; Patel,Sachin; Patel,Hetal; Pandey,Sonia; Shah,Shailesh; Shah,Dinesh.
The present study describes the development and validation of a dissolution method for carvedilol compression-coated tablets. Dissolution test was performed using a TDT-06T dissolution apparatus. Based on the physiological conditions of the body, 0.1N hydrochloric acid was used as dissolution medium and release was monitored for 2 hours to verify the immediate release pattern of the drug in acidic pH, followed by pH 6.8 in citric-phosphate buffer for 22 hours, to simulate a sustained release pattern in the intestine. Influences of rotation speed and surfactant concentration in medium were evaluated. Samples were analysed by validated UV visible spectrophotometric method at 286 nm. 1% sodium lauryl sulphate (SLS) was found to be optimum for improving...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carvedilol/coated tablets; Compression coated tablets/dissolution; PH 6.8 citric-phosphate buffer; Hydrochloric acid; Sodium lauryl sulphate.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400027

Obesity, inflammation, and insulin resistance
Provedor de dados: 60
Autores: Martins,Luana Mota; Oliveira,Ana Raquel Soares; Cruz,Kyria Jayanne Clímaco; Torres-Leal,Francisco Leonardo; Marreiro,Dilina do Nascimento.
White adipose tissue (WAT) is considered an endocrine organ. When present in excess, WAT can influence metabolism via biologically active molecules. Following unregulated production of such molecules, adipose tissue dysfunction results, contributing to complications associated with obesity. Previous studies have implicated pro- and anti-inflammatory substances in the regulation of inflammatory response and in the development of insulin resistance. In obese individuals, pro-inflammatory molecules produced by adipose tissue contribute to the development of insulin resistance and increased risk of cardiovascular disease. On the other hand, the molecules with anti-inflammatory action, that have been associated with the improvement of insulin sensitivity, have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Inflammation; Adipose tissue; Adipokines; Insulin resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400677

WBC count and functional changes induced by co-administration of clofazimine and clarithromycin, in single and multiple doses, in Wistar rats
Provedor de dados: 60
Autores: Paina,Flávia Aparecida; Miranda,Julise Cunha; Tavares,Cristiane Fernandes de Freitas; Queiroz,Regina Helena da Costa; Souza,Ana Maria de.
Clofazimine and clarithromycin are used to treat leprosy and infections caused by Mycobacterium avium complex. Little data on the toxicity of co-administration of these two drugs are available. Here we evaluated the potential adverse effects of polytherapy with these two drugs in male Wistar rats by determining WBCs counts and other blood cell counts, neutrophilic phagocytosis, and burst oxidative, by flow cytometry. We observed an increase in WBCs, in multiple-dose regimens, and in polymorphonuclear cells, in both single- clarithromycin only and multiple dose regimens. We also observed a reduction in mononuclear cell counts in single and multiple doses. The drugs seem to reverse the mononuclear and polymorphonuclear cell ratio. An increase in oxidative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clofazimine/adverse effects; Clofazimine/experimental study; Clarithromycin/adverse effects; Clarithromycin/experimental study; Leprosy/treatment; Mycobacterium avium/infections; Mycobacterium avium/treatment; Leukocytes; Phagocytosis; Oxidative burst.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300008

Amiodarone hydrochloride: enhancement of solubility and dissolution rate by solid dispersion technique
Provedor de dados: 60
Autores: Rubim,Alexandre Machado; Rubenick,Jaqueline Bandeira; Gregolin,Eduarda; Laporta,Luciane Varini; Leitenberg,Rosimar; Rolim,Clarice Madalena Bueno.
abstract Amiodarone HCl is an antiarrhythmic agent, which has low aqueous solubility and presents absorption problems. This study aimed to develop inclusion complexes containing hydrophilic carriers PEG 1500, 4000 and 6000 by fusion and kneading methods in order to evaluate the increase in solubility and dissolution rate of amiodarone HCl. The solid dispersion and physical mixtures were characterized by X-ray diffraction, FT-IR spectra, water solubility and dissolution profiles. Both methods and carriers increased the solubility of drug, however PEG 6000 enhanced the drug solubility in solid dispersion better than other carriers. Different media were evaluated for the solubility study, including distilled water, acid buffer pH 1.2, acetate buffer pH 4.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amiodarone hydrochloride/dissolution profile; Amiodarone hydrochloride/solid dispersion; Hydrophilic polymer.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400957

Solubility enhancement, physicochemical characterization and formulation of fast-dissolving tablet of nifedipine-betacyclodextrin complexes
Provedor de dados: 60
Autores: Jagdale,Swati Changdeo; Jadhav,Vinayak Narhari; Chabukswar,Aniruddha Rajaram; Kuchekar,Bhanudas Shankar.
The main objective of the study was to enhance the dissolution of nifedipine, a poorly water soluble drug by betacyclodextrin complexation and to study the effect of the preparation method on the in vitro dissolution profile. The stoichiometric ratio determined by phase solubility analysis for inclusion complexation of nifedipine with β-cyclodextrin was 1:1. Binary complex was prepared by different methods and was further characterized using XRD, DSC and FT-IR. A saturation solubility study was carried out to evaluate the increase in solubility of nifedipine. The optimized complex was formulated into fast-dissolving tablets by using the superdisintegrants Doshion P544, pregelatinized starch, crospovidone, sodium starch glycolate and croscarmellose sodium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fast-dissolving tablets; Nifedipine/dissolution; Β-ciclodextrin/complexation; Superdisintegrants.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100015

Insulin-loaded polymeric mucoadhesive nanoparticles: development, characterization and cytotoxicity evaluation
Provedor de dados: 60
Autores: Gatti,Tiago Henrique Honorato; Eloy,Josimar Oliveira; Ferreira,Leonardo Miziara Barboza; Silva,Isabel Cristine da; Pavan,Fernando Rogério; Gremião,Maria Palmira Daflon; Chorilli,Marlus.
Abstract Mucoadhesive nanoparticles are particularly interesting for delivery through nasal or pulmonary routes, as an approach to overcome the mucociliary clearance. Moreover, these nanoparticles are attractive for peptide and protein delivery, particularly for insulin to treat diabetes, as an alternative to conventional parenteral administration. Thus, chitosan, a cationic mucoadhesive polysaccharide found in shells of crustaceans, and the negatively-charged dextran sulfate are able to form nanoparticles through ionic condensation, representing a potential insulin carrier. Herein, chitosan/dextran sulfate nanoparticles at various ratios were prepared for insulin loading. Formulations were characterized for particle size, zeta potential, encapsulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Chitosan; Dextran-Sulfate; Nanoparticles; Mucoadhesion.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100623

Strategies towards expansion of chemical space of natural product-based compounds to enable drug discovery
Provedor de dados: 60
Autores: Silva,Daniel Gedder; Emery,Flavio da Silva.
Natural products (NPs) are an excellent source of biologically active molecules that provide many biologically biased features that enable innovative designing of synthetic compounds. NPs are characterized by high content of sp3-hybridized carbon atoms; oxygen; spiro, bridged, and linked systems; and stereogenic centers, with high structural diversity. To date, several approaches have been implemented for mapping and navigating into the chemical space of NPs to explore the different aspects of chemical space. The approaches providing novel opportunities to synthesize NP-inspired compound libraries involve NP-based fragments and ring distortion strategies. These methodologies allow access to areas of chemical space that are less explored, and consequently...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical space; Natural products; Ring distortion; Fragment-based drug discovery; Diversity-oriented synthesis; Chemical degradation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700403

Exposure to potentially inappropriate medications in Brazilian elderly outpatients with metabolic diseases
Provedor de dados: 60
Autores: Martins,Vanessa dos Santos; Mori,Ana Luiza Pereira Moreira; Dorea,Egidio Lima; Pinto,Gelba Almeida; Hirata,Mario Hiroyuki; Hirata,Felipe Dominguez Crespo; Hirata,Rosario Dominguez Crespo.
ABSTRACT Management of pharmacotherapy in elderly with metabolic diseases is challenging and potentially inappropriate medications (PIMs) are risk factors for drug interactions and adverse events. The exposure to PIMs in elderly outpatients with metabolic diseases and its relationship with polypharmacy and other variables was investigated. PIMs prescribed to 207 elderly patients (aged 60 to 96 years) with metabolic diseases who attended a University Hospital of Sao Paulo city, Brazil, from April/2010 to January/2011, were evaluated. PIMs were detected using both 2003 Beers and 2008 STOPP criteria. The association between PIMs and age, gender and polypharmacy was also examined. 2008 STOPP criteria detected more PIMs (44.4 %) than 2003 Beers criteria (16.0%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potentially inappropriate medications/elderly/effects; Elderly/metabolic diseases/study; Pharmacotherapy/elderly; Beers criteria; STOPP criteria; Polypharmacy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400699

Synthesis and in vitro anti-HIV-1 evaluation of some N-arylsulfonyl-3-formylindoles
Provedor de dados: 60
Autores: Che,Zhiping; Tian,Yuee; Liu,Shengming; Hu,Mei; Chen,Genqiang.
As our ongoing work on research of anti-HIV-1 inhibitors, fifteen N-arylsulfonyl-3-formylindoles (3a-o) were designed and prepared through two step synthetic route. Firstly, 3-formylindoles (2a-c) were synthesized via the Vilsmeier-Haack reaction. Subsequently, treatment of 2a-c with the appropriate arylsulfonyl chlorides led to the corresponding target compounds in excellent yields. All analogues were also preliminary evaluated in vitro for their inhibitory activity against HIV-1 replication. Among of all the reported analogues, three compounds 3c, 3g and 3i displayed significant anti-HIV-1 activity, with EC50 values of 9.57, 11.04 and 5.02 μM, and TI values of 31.89, 13.79 and 81.69, respectively. N-m-nitrophenylsulfonyl-3-formylindole (3c) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N-Arylsulfonyl-3-formylindole/synthesis; Human immunodeficiency virus type-1/inhibitor.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300605

Osteomyelitis: an overview of antimicrobial therapy
Provedor de dados: 60
Autores: Gomes,Diana; Pereira,Margarida; Bettencourt,Ana Francisca.
Osteomyelitis is an inflammatory bone disorder caused by infection, leading to necrosis and destruction of bone. It can affect all ages, involve any bone, become a chronic disease and cause persistent morbidity. Treatment of osteomyelitis is challenging particularly when complex multiresistant bacterial biofilm has already been established. Bacteria in biofilm persist in a low metabolic phase, causing persistent infection due to increased resistance to antibiotics. Staphylococcus aureus and Staphylococcus epidermidis are the most common causative organism responsible for more than 50% of osteomyelitis cases. Osteomyelitis treatment implies the administration of high doses of antibiotics (AB) by means of endovenous and oral routes and should take a period...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteomyelitis/pharmacological treatment; Osteomyelitis/antimicrobial therapy; Staphylococcus aureus/presence; Staphylococcus aureus/Osteomyelitis; Antibiotics/use; Antibiotics/osteomyelitis treatment.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100003

Effects of nanosuspension and inclusion complex techniques on the in vitro protease inhibitory activity of naproxen
Provedor de dados: 60
Autores: Dharmalingam,Senthil Rajan; Chidambaram,Kumarappan; Ramamurthy,Srinivasan; Nadaraju,Shamala.
This study investigated the effects of nanosuspension and inclusion complex techniques on in vitro trypsin inhibitory activity of naproxen—a member of the propionic acid derivatives, which are a group of antipyretic, analgesic, and non-steroidal anti-inflammatory drugs. Nanosuspension and inclusion complex techniques were used to increase the solubility and anti-inflammatory efficacy of naproxen. The evaporative precipitation into aqueous solution (EPAS) technique and the kneading methods were used to prepare the nanosuspension and inclusion complex of naproxen, respectively. We also used an in vitro protease inhibitory assay to investigate the anti-inflammatory effect of modified naproxen formulations. Physiochemical properties of modified naproxen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naproxen/nanosuspension/anti-inflammatory activity; Naproxen/inclusion complex/anti-inflammatory activity; Naproxen/solubility; Protease inhibition.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100017

Single gas chromatography method with nitrogen phosphorus detector for urinary cotinine determination in passive and active smokers
Provedor de dados: 60
Autores: Malafatti,Lusiane; Maia,Patrícia Penido; Martins,Matheus Coutinho Gonçalves; Siqueira,Maria Elisa Pereira Bastos de; Martins,Isarita.
Nicotine is a major addictive compound in cigarettes and is rapidly and extensively metabolized to several metabolites in humans, including urinary cotinine, considered a biomarker due to its high concentration compared to other metabolites. The aim of this study was to develop a single method for determination of urinary cotinine, in active and passive smokers, by gas chromatography with a nitrogen phosphorus detector (GC-NPD). Urine (5.0 mL) was extracted with 1.0 mL of sodium hydroxide 5 mol L-1, 5.0 mL of chloroform, and lidocaine used as the internal standard. Injection volume was 1 μL in GC-NPD. Limit of quantification was 10 ng mL-1. Linearity was evaluated in the ranges 10-1000 ng mL-1 and 500-6000 ng mL-1, with determination coefficients of 0.9986...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gas chromatography/with nitrogen phosphorus detector; Urinary cotinine/determination; Urine/toxicological analysis; Smokers/Toxicology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400019

Leaf and stem microscopic identification of Tithonia diversifolia (Hemsl.) A. Gray (Asteraceae)
Provedor de dados: 60
Autores: Duarte,Márcia do Rocio; Empinotti,Cláudia Bonissoni.
Tithonia diversifolia (Hemsl.) A. Gray is an Asteraceae shrub, popularly known as Mexican sunflower and cultivated for ornamental and therapeutic uses in different countries. In folk medicine, it is of value for treating diabetes, malaria and infectious diseases. These indications have been corroborated by various pharmacological assays. Given the lack of data on anatomical aspects of T. diversifolia, this work aimed to investigate the leaf and stem microscopic characters of this medicinal plant and potential vegetal drug. Samples of mature leaves and young stems were sectioned and stained. Histochemical tests and scanning electron microscopy were also performed. The leaf has anomocytic stomata on both sides, dorsiventral mesophyll and several collateral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tithonia diversifolia/pharmacognosy; Tithonia diversifolia/anatomical characters; Medicinal plants; Mexican sunflower.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100013

Characterization of bacterial endophytes from Myanmar medicinal plants for antimicrobial activity against human and plant pathogens
Provedor de dados: 60
Autores: Myo,Ei Mon; Maung,Chaw Ei Htwe; Mya,Khin Mar; Khai,Aye Aye.
This research aimed to investigate the antagonistic activity of the bacterial endophytes from Myanmar medicinal plants. Thirty-one bacterial isolates were isolated from Myanmar medicinal plants: Tinospora cordifolia (Wild.) Miers., Catharanthus roseus G. Don., Tectona hamiltoniana Wall. and Boscia variabilis Collett &amp; Hemsl. (Capparaceae). Dual culture and agar well diffusion methods were used for antimicrobial assay. One endophyte from Catharanthus roseus and two bacterial isolates from Boscia variabilis Collett &amp; Hemsl. (Capparaceae) had not only the antibacterial activity towards the human pathogenic bacteria but also the antifungal activity against the plant pathogenic fungi. From 16S rRNA sequencing, one strain from Catharanthus roseus G. Don....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial; Antifungal; Culture conditions; Endophytes; Medicinal plants.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100513

Development, characterization and evaluation of the dissolution profile of sulfasalazine suspensions
Provedor de dados: 60
Autores: Costa,Mayre Aparecida Borges da; Villa,Ana Lucia Vazquez; Barros,Rita de Cássia da Silva Ascenção; Ricci-Júnior,Eduardo; Santos,Elisabete Pereira dos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sulfasalazine/suspensions/dissolution; Chronic intestinal inflammatory diseases/treatment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200449

Preparation and evaluation of azithromycin binary solid dispersions using various polyethylene glycols for the improvement of the drug solubility and dissolution rate
Provedor de dados: 60
Autores: Adeli,Ehsan.
ABSTRACT Azithromycin is a water-insoluble drug, with a very low bioavailability. In order to increase the solubility and dissolution rate, and consequently increase the bioavailability of poorly-soluble drugs (such as azithromycin), various techniques can be applied. One of such techniques is "solid dispersion". This technique is frequently used to improve the dissolution rate of poorly water-soluble compounds. Owing to its low solubility and dissolution rate, azithromycin does not have a suitable bioavailability. Therefore, the main purpose of this investigation was to increase the solubility and dissolution rate of azithromycin by preparing its solid dispersion, using different Polyethylene glycols (PEG). Preparations of solid dispersions and physical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azithromycin/solubility; Azithromycin/dissolution; Solid dispersion; Polyethylene glycols.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100002

A model for drug dispensing service based on the care process in the Brazilian health system
Provedor de dados: 60
Autores: Soares,Luciano; Diehl,Eliana Elisabeth; Leite,Silvana Nair; Farias,Mareni Rocha.
Access to medication emphasizes the availability of the product at the expense of providing a service. The goal of this paper is to propose a theoretical model for a drug dispensing service, beginning with a reflection on the current realities of the Unified Health System and drug dispensation in Brazil. A conceptual analytical research made by a methodological course called disciplined imagination was mainly the approach applied to develop the model. The drug dispensing service is part of the care process, which considers access as an attribute; reception, connection and accountability, management, and clinical pharmaceutical aspects as components; and the rational use of drugs as the purpose. The proposed model addresses access to the dispensing service...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/dispensing practice; Pharmaceutical service; Unified health system; Health services/access.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100012

Comparison of dissolution profile of extended-release oral dosage forms - two one-sided equivalence test
Provedor de dados: 60
Autores: Lourenço,Felipe Rebello; Ghisleni,Daniela Dal Molim; Yamamoto,Rosa Noriko; Pinto,Terezinha de Jesus Andreoli.
The aim of this work is to present the two one-sided test (TOST) as an alternative approach to compare dissolution profiles of extended-release dosage forms. The dissolution profiles of oxycodone extended-release tablets containing 10 mg, 20 mg and 40 mg (reference and generic) were evaluated according to the requirements described in United States Pharmacopeia. These dissolution profiles were compared using the conventional similarity factor (f2) and the proposed TOST as an equivalence test. TOST is a simple and alternative approach to compare dissolution profiles of extended-release dosage forms. It allows us to identify the time-point (or time-points) that did not show similarity. We concluded that the two one-sided test performed at a significance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dissolution profile; Extended-release tablets; Similarity factor; Two one-sided test; Equivalence test.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200019

Ethanolic extract of Passiflora edulis Sims leaves inhibits protein glycation and restores the oxidative burst in diabetic rat macrophages after Candida albicans exposure
Provedor de dados: 60
Autores: Martins,Carolina Fernandes Ribas; Salles,Bruno César Corrêa; Brigagão,Maisa Ribeiro Pereira Lima; Rodrigues,Maria Rita; Ferreira,Eric Batista; Duarte,Stella Maris da Silveira; Paula,Fernanda Borges de Araujo.
abstract This study was conducted to evaluate the effects of the ethanolic extract of Passiflora edulis leaves on blood glucose, protein glycation, NADPH oxidase activity and macrophage phagocytic capacity after Candida albicans exposure in diabetic rats. The Passiflora edulis Sims leaves were dried to 40°C, powdered, extracted by maceration in 70% ethanol, evaporated under reduced pressure and lyophilised. The biochemical tests performed were total phenolic content (TP) as determined by the Folin-Ciocalteu assay, trapping potential DPPH assay and total iron-reducing potential. Diabetes was induced by alloxan injection. Protein glycation was determined by AGE and fructosamine serum concentrations. Extract-treated diabetic animals demonstrated lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis Sims/pharmacognosy; Passiflora edulis Sims/ethanolic extract/effects; Blood glucose; Protein glycation; Reactive oxygen species; Diabetes/treatment/experimental study; Medicinal plants.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400869

Marine functional food
Provedor de dados: 60
Autores: Castro,Inar Alves de.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400026

Development and validation of an electroanalytical methodology for determination of isoniazid and rifampicin content in pharmaceutical formulations
Provedor de dados: 60
Autores: Leandro,Katia Christina; Carvalho,Juliana Machado de; Giovanelli,Luiz Fernando; Moreira,Josino Costa.
Tuberculosis remains a major public health problem, especially in developing countries. Brazil presents the largest number of cases in Latin America and is among the 22 countries considered priorities by the World Health Organization (WHO). The Rio de Janeiro state has the largest number of cases registered in the country. The treatment of patients, commonly, makes use of the drugs isoniazid and rifampicin for six months. This study aimed to develop and validate an electroanalytical methodology, using the technique of differential pulse voltammetry for the determination of these drugs in the associated form, in order to evaluate the quality of medicines distributed in the state of Rio de Janeiro. The potential reduction for the isoniazid and rifampicin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoniazid; Rifampicin; Tuberculosis/treatment; Tuberculostatics/quantitative analysis; Differential pulse voltammetry.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200019

Chemical waste risk reduction and environmental impact generated by laboratory activities in research and teaching institutions
Provedor de dados: 60
Autores: Nascimento,Elizabeth de Souza; Tenuta Filho,Alfredo.
The environmental impact caused by teaching and research with regard to chemical waste is of increasing concern, and attempts to solve the issue are being made. Education and research-related institutions, in most laboratory and non-laboratory activities, contribute to the generation of small quantities of waste, many of them highly toxic. Of this waste, some is listed by government agencies who are concerned about environmental pollution: disposal of acids, metals, solvents, chemicals and toxicity of selected products of synthesis, whose toxicity is often unknown. This article presents an assessment of the problem and identifies possible solutions, indicating pertinent laws, directives and guidelines; examples of institutions that have implemented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laboratory chemical waste/management in universities; Environmental safety; Environmental education.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200004

Pharmacokinetic-pharmacodynamic correlation of imipenem in pediatric burn patients using a bioanalytical liquid chromatographic method
Provedor de dados: 60
Autores: Santos,Silvia Regina Cavani Jorge; Sanches-Giraud,Cristina; Silva Júnior,Carlindo Vieira; Gomez,David Souza.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Imipenem/quantification; Antibiotics/dosage; Pediatric patients/burn/antibiotics use; High Performance Liquid Chromatography/quantitative analysis; Drug plasma monitoring; PK/PD.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200305

Validation of analytical methodology for quantification of cefazolin sodium pharmaceutical dosage form by high performance liquid chromatography to be applied for quality control in pharmaceutical industry
Provedor de dados: 60
Autores: Pedroso,Tahisa Marcela; Salgado,Hérida Regina Nunes.
A reversed-phase high performance liquid chromatography method was validated for the determination of cefazolin sodium in lyophilized powder for solution for injection to be applied for quality control in pharmaceutical industry. The liquid chromatography method was conducted on a Zorbax Eclipse Plus C18 column (250 x 4.6 mm, 5 μm), maintained at room temperature. The mobile phase consisted of purified water: acetonitrile (60: 40 v/v), adjusted to pH 8 with triethylamine. The flow rate was of 0.5 mL min-1 and effluents were monitored at 270 nm. The retention time for cefazolin sodium was 3.6 min. The method proved to be linear (r2=0.9999) over the concentration range of 30-80 µg mL-1. The selectivity of the method was proven through degradation studies....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cefazolin sodium/determination; High performance liquid chromatography/reverse-phase/quantitative analysis; Pharmaceutical industry/quality control; Cefalosporine/quality control; Medicines/quality control.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100022

Effects of gallic acid and physical exercise on passive avoidance memory in male rat
Provedor de dados: 60
Autores: Salehi,Azadeh; Rabiei,Zahra; Setorki,Mahbubeh.
Learning and memory play main roles in daily life of human, and memory represents the basis of all trainings and learning. The aim of the current study is to investigate the effects of gallic acid and physical exercise on the levels of passive avoidance memory in rat. In this experimental study, 46 rats weighing 200-300 g were randomLy divided to six groups of eight each: including control group, groups treated with 10 and 20 mg/kg gallic acid, group undergoing physical exercise alone, and groups both undergoing physical exercise and treated with 10 and 20 mg/kg gallic acid. The interventions continued for 10 days. After the intervention, passive avoidance memory was measured by shuttle box, blood samples were taken, and serum and brain antioxidant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallic Acid/adverse effects; Learning/drug effects; Memory/drug effects; Physical Exercise/physiology; MDA; Avoidance Learning/drug effects; Exercise/physiology; Rats/physiology; Malondialdehyde/analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100568

Method development and validation for naratriptan determination in human plasma by HPLC with tandem mass spectrometry detection, and its application to bioequivalence study
Provedor de dados: 60
Autores: Challa,Balasekhara Reddy; Awen,Bahlul Zayed Shtaiwy; Chandu,Babu Rao; Shaik,Rihana Parveen.
The authors developed a simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of naratriptan (NP) in human plasma using naratriptan-d3 (NPD3) as an internal standard (IS). Chromatographic separation was performed on a Zorbax SB-C18, 75 x 4.6 mm, 3.5 µm column with an isocratic mobile phase composed of 0.1% formic acid : acetonitrile (50:50 v/v), at a flow-rate of 0.6 mL/min. NP and NPD3 were detected with proton adducts at m/z 336.5→98.0 and 339.4→101.0 in selected reaction monitoring (SRM) positive mode, respectively. The liquid-liquid extraction method was used to extract the NP and NPD3. This method was validated over a linear concentration range of 0.1-25.0 ng/mL with a correlation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liquid chromatography/quantitative analysis; Mass spectrometry/quantitative analysis; Naratriptan/determination; Naratriptan/human plasma.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100003

A novel 96-microwell-based high-throughput spectrophotometric assay for pharmaceutical quality control of crizotinib, a novel potent drug for the treatment of non-small cell lung cancer
Provedor de dados: 60
Autores: Wani,Tanveer Ahmed; Darwish,Ibrahim Ali.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crizotinib/capsules/quality control 1; 2-Naphthoquinone-4-sulphonate Spectrophotometry/quality control of medicines.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200439

Pharmaceutical assistance in local public health services in Santa Catarina (Brazil): characteristics of its organization
Provedor de dados: 60
Autores: Veber,Ana Paula; Diehl,Eliana; Leite,Silvana Nair; Prospero,Elisete Navas Sanches.
This study outlines the diagnosis stage of 201 Municipal Pharmaceutical Assistance Plans sent to the Directorate of Pharmaceutical Assistance of the State Secretariat for Health of Santa Catarina (DIAF-SES/SC) between November 12, 2005 and July 6, 2006. Multiple Correspondence Analysis was used in conjunction with SPAD 3.5 software, followed by hypothesis testing. The variables "pharmacist presence" and "population" were those which most contributed to the formation of the three clusters of municipalities observed. The number of drugs contained in the Municipal Lists of Essential Drugs (REMUMEs) ranged from 15 to 413. A total of 67 towns had between 101 and 200 drugs- a range considered ideal in this study according to the 2006 National List of Essential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical assistance/services; Health management; Primary health care.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100009

Validation of a liquid chromatographic method for determination of related substances in a candidate certified reference material of captopril
Provedor de dados: 60
Autores: Nogueira,Raquel; Wollinger,Wagner; Silva,Thaís Elias da; Oliveira,Leonardo Mesquita de; Rego,Eliane Cristina Pires do; Moreira,Gabriela Fernandes; Barin,Juliano Smanioto; Laporta,Luciane Varini; Mesko,Márcia Foster; Bittencourt,Celso Figueiredo; Rodrigues,Janaína Marques; Cunha,Valnei Smarçaro da.
This paper describes the validation of a reversed-phase high performance liquid chromatography method (RP-HPLC) with diode array detection (DAD) for determination of related substances (impurities from organic synthesis and degradation products) of captopril according to the Brazilian Pharmacopeia IV. The aim of this study was to guarantee the method accuracy for quantification of related substances, an essential requisite to determine, using the mass balance approach, the captopril content in the first Brazilian certified reference material (CRM) of an active pharmaceutical ingredient (API), developed by Inmetro. The captopril instability in solution is discussed and the captopril content determined by mass balance is compared to the results from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Captopril/qualitative analysis; Medicines/analysis; Medicines/method validation; Medicines/analytical quality control; High performance liquid chromatography/qualitative analysis; Medicines/contamination; Captopril/certified reference standards.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200016

Validation of spectrophotometric method to detect and quantify nitrite in ham pate
Provedor de dados: 60
Autores: Reis,Patrícia Barros; Ramos,Rodrigo Mauro; Souza,Leonardo Francisco de; Cançado,Silvana de Vasconcelos.
The objective of this work was to validate the spectrophotometric method to detect and quantify nitrite using ham pate as a source. The validated analytical conditions were 540 nm wavelength and the samples reading between 40 to 70 minutes after addition of coloring agents. The tested performance criteria were: linearity, matrix effect, selectivity, detection and quantification limit, accuracy, precision and robustness. As the results have obtained linearity in the studied zone (0.125 to 3 g/mL) and matrix effect was not observed, there was not any interference from ascorbic acid, but interference occurred from sodium eritorbate, in concentrations above 2.5 mg/100g. The detection limit was defined as 12.5 mg of nitrite/kg for pate and the quantification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Validation; Nitrite; Spectrophotometric method; Ham pate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100006

Passiflora incarnata treatment during gestation and lactation: toxicological and antioxidant evaluation in wistar dams
Provedor de dados: 60
Autores: Boll,Karine Maria; Bortolasci,Chiara Cristina; Zaminelli,Tiago; Veríssimo,Luiz Fernando; Bacchi,André Demambre; Higachi,Luciana; Barbosa,Décio Sabbatini; Moreira,Estefânia Gastaldello.
Passiflora incarnata is marketed in many countries as a phytomedicine. Even though the directions of most marketed phytomedicines recommend them to be used under medical supervision, reproductive and developmental studies are sparse and not mandatory for regulatory purposes. In this study, a reproductive toxicity evaluation of P. incarnata was conducted in Wistar rats gavaged (30 or 300 mg/kg) during pregnancy and lactation. Moreover, considering that antioxidant properties have been attributed to flavonoids present in the genus Passiflora, it was also evaluated the antioxidant/pro-oxidant balance in the plasma of these dams and the antioxidant potential in an in vitro test. P. incarnata treatment did not influence dams´ body weight as well as reproductive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytomedicines/evaluation; Passiflora incarnata/use in pregnancy; Passiflora incarnata/use in lactation; Passiflora incarnata/reproductive toxicity/experimental study; Pregnancy/use of phytomedicines; Lactation/use of phytomedicines; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200353

Development and validation of a HPLC analytical assay method for efavirenz tablets: a medicine for HIV infections
Provedor de dados: 60
Autores: Viana,Osnir de Sá; Medeiros,Flávia Patrícia Morais; Grangeiro-Júnior,Severino; Albuquerque,Miracy Muniz; Soares,Mônica Felts La Roca; Soares-Sobrinho,José Lamartine; Alves,Lariza Darlene Santos; Rolim,Larissa Araújo; Silva,Keyla Emanuelle Ramos da; Rolim-Neto,Pedro José.
Efavirenz is a reverse transcriptase non analog nucleoside inhibitor used to treat HIV infections. A simple assay method by high performance liquid chromatography was developed and validated for efavirenz tablets. The physical chemical characteristics of efavirenz were investigated to developing the method. The method was validated observing the parameters described in USP 29. Analyses were performed by an ultraviolet detector at a 252 nm wavelength, on a reverse-phase column (C18, 250 mm x 3.9 mm, 10 μm), using an isocratic mobile phase containing acetonitrile/water/orthophosphoric acid (70:30:0.1). The validation parameters used were: selectivity, linearity, precision, accuracy, robustness, detection and quantification limits, and all resulting data were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Efavirenz/tablet assay; High performance liquid chromatography/quantitative analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100012

Gd-GLU toward NMR imaging: synthesis, characterization and breast cell uptake assay
Provedor de dados: 60
Autores: Campos,Tarcisio Passos Ribeiro de; Dalmazio,Ilza; Augusti,Rodiney; Almeida,Iassudara Garcia.
Breast cancer cell uptake of Gd-metal is investigated based on the formation of coordinate compounds of gadolinium and glucose (Glu) molecules in solution. The hypothesis is that glucose helps Gd-internalization by complex formations constituted of Gd3+ coordinate to m-glucose molecules, whose valence was complemented by Cl- anions. Such a proposal is an insight toward a metabolic-dependent contrast-agent for cancer and inflammation in magnetic resonance image. A solution was prepared based on anhydrous d-glucose and gadolinium chloride (Gd-Glu). Uptake assays for MDA-MB-231(c231) cells were elaborated collecting incubated c231-cells with Gd-Glu and measuring metal-uptake and their concentrations by Nuclear Activation Analysis (NAA). The ionic solution was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose; Gadolinium; Breast cancer; In vitro uptake; MDA-MB-231.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100527

Validation of impregnation process for homeopathic globules by spectrophotometric-UV method
Provedor de dados: 60
Autores: Pinheiro,Mariana Santos; Aguiar,Paula Fernandes de; Quaresma,Carla Holandino; Garcia,Sheila.
We compared the impregnation techniques for globules according to the Manual of Technical Norms for Homeopathic Pharmacies (MNTFH) of the Association of Homeopathic Pharmacists (ABFH), Brazilian Homeopathic Pharmacopoeia (FHB) and variations of these techniques. The variables were evaluated in this procedure, three different sizes of globules (N o . 3, 5 and 7), the hydroalcoholic solution of 70% (v/v) Minoxidil 2% (w/v) was used to impregnate the globules in concentrations of 2, 3, 4, 5% (v/w) and the impregnation at 10 %(v/w) was used hydroalcoholic solutions at 70, 80 and 90% (v/v), and four impregnation techniques various ( A -glass, B -paper, C -cup and D -FHB). As the results of content uniformity did not demonstrate a normal distribution, the one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homeopathic globules/impregnation process; Homeopathic compounding/evaluation; Homeopathic dose/uniformity of content.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100014

Enteric coated HPMC capsules plugged with 5-FU loaded microsponges: a potential approach for treatment of colon cancer
Provedor de dados: 60
Autores: Gupta,Ankita; Tiwari,Gaurav; Tiwari,Ruchi; Srivastava,Rishabh; Rai,A. K.
The work was aimed at developing novel enteric coated HPMC capsules (ECHC) plugged with 5 Florouracil (5-FU) loaded Microsponges in combination with calcium pectinate beads. Modified quasi-emulsion solvent diffusion method was used to formulate microsponges based on 32 factorial design and the effects of independent variables (volume of organic solvent and Eudragit RS100 content) on the dependent variables (Particle size, %EE &amp; % CDR) were determined. The optimized microsponges (F4) were characterized by SEM, PXRD, TGA and were plugged along with calcium pectinate beads in HPMC capsules and the HPMC capsules were further coated with enteric polymer Eudragit L 100 (Ed-L100) and/ or Eudrgit S 100 (Ed-S 100) in different proportions. In vitro release...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5-Fluorouracil; HPMC capsules/enteric coating; Microsponges/formulation; Drugs/delivery/colon targeting; Colorectal cancer/treatment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300591

Gas Chromatography-Mass Spectrometry (GC-MS) and evaluation of antioxidant and antimicrobial activities of essential oil of Campomanesia adamantium (Cambess.) O. Berg (Guavira)
Provedor de dados: 60
Autores: Coutinho,Isabel Duarte; Cardoso,Claúdia Andréa Lima; Ré-Poppi,Nilva; Melo,Adriana Mestriner; Vieira,Maria do Carmo; Honda,Neli Kika; Coelho,Roberta Gomes.
The essential oils from Campomanesia adamantium (Cambess.) O. Berg leaves, collected in the reproductive (flowering and fruit-bearing) and vegetative stages, were characterized by GC-MS (Gas Chromatography-Mass Spectrometry). A total of 95 compounds of the essential oils were identified. In the reproductive stage (flowering) the major constituents were monoterpenes (limonene, α-pinene and β-pinene) while during the vegetative stage the major constituents were the sesquiterpenes (bicyclogermacrene and globulol). The essential oil of the reproductive stage shows high antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, and all show moderate activity against Escherichia coli. The essential oils were also evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campomanesia adamantium/antioxidant activity; Campomanesia adamantium/antimicrobial activity; Guavira; Essential oil/caracterization.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400022

Preparation and characterization of microcapsules of Pterodon pubescens Benth. by using natural polymers
Provedor de dados: 60
Autores: Reinas,Alexandre Espada; Hoscheid,Jaqueline; Outuki,Priscila Miyuki; Cardoso,Mara Lane Carvalho.
An oleaginous fraction obtained from an alcohol extract of the fruit of Pterodon pubescensBenth. (FHPp) was microencapsulated in polymeric systems. These systems were developed using a complex coacervation method and consisted of alginate/medium-molecular-weight chitosan (F1-MC), alginate/chitosan with greater than 75% deacetylation (F2-MC), and alginate/low-molecular-weight chitosan (F3-MC). These developed systems have the potential to both mask the taste of the extract, and to protect its constituents against possible chemical degradation. The influence of the formulation parameters and process were determined by chemical profiling and measurement of the microencapsulation efficiency of the oleaginous fraction, and by assessment of microcapsule...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pterodon pubescensBenth./pharmacognosy; Sucupira; Microcapsules/preparation; Vouacapans; Alginate; Chitosan; Coacervation method; Natural polymers; Natural products.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400919

Formulation and evaluation of transdermal drug-delivery system of isosorbide dinitrate
Provedor de dados: 60
Autores: Zhan,Xiaoping; Mao,Zhenmin; Chen,Sijing; Chen,Shaoxiong; Wang,Liqun.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isosorbide dinitrate/transdermal drug-delivery system; Transdermal patches/in vitro release; Transdermal patches/sustained release; Controlling membrane/drugs release rate; Skin penetration/drugs release.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200373

Amphotericin-B and vancomycin-loaded chitosan nanofiber for antifungal and antibacterial application
Provedor de dados: 60
Autores: Karimi,Soroush; Moradipour,Pouran; Azandaryani,Abbas Hemati; Arkan,Elham.
In the present study, a mucoadhesive non-woven fiber mat (d= 116 nm) was fabricated by the electrospinning method using chitosan (80% Wt), polyethylene oxide (10% Wt), cysteine (4% Wt) and drugs (6% Wt), respectively. In addition, a comparative study was conducted to define effect of drugs and mucoadhesive agent on the nanofiber formation. FTIR, SEM, DSC and DMA were used to investigate the chemical and physical properties of the nanofibers. In vitro release of the drugs was assessed over a 48-hour period by the total immersion method. Release data were ﬁtted to kinetic models, including the zero-order, ﬁrst-order, Higuchi matrix, and Hixson-Crowell. Zone inhibition investigations were used to describe the inhibition content of vancomycin and amphotericin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucoadhesive; Vancomycin; Amphotericin-B; Electrospinning; Aphthous; Chitosan.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100521

Preparation and physicochemical characterization of prazosin conjugated PLGA nanoparticles for drug delivery of flutamide
Provedor de dados: 60
Autores: Fattahi,Ali; Ghiasi,Mansoureh; Mohammadi,Pardis; Hosseinzadeh,Leila; Adibkia,Khosro; Mohammadi,Ghobad.
In the current work, a sustained drug delivery system of flutamide (FLT) was developed using Poly(D,L-lactide-co-glycolide) (PLGA) decorated bypoly(ethylene glycol) (PEG) grafted prazosin (PLGA-PEG-Praz) as a targeting moiety. In a multi-step reaction, PLGA was linked to PEG and prazosin. The structure of the synthesized polymers was confirmed by FTIR and 1H-NMR. Flutamide-loaded nanoparticles were prepared by quasi-emulsion solvent diffusion technique. The nanoparticles were evaluated for size, zeta potential, polydispersity index, drug crystallinity, loading efficiency, and release properties. Also, the physicochemical properties of the nanoparticles were analyzed using Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry, and Powder...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticle; PLGA; Flutamide; Prazosin; Prostate cancer; Drug delivery.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400609

Consumption of medicines in high-risk pregnancy: evaluation of determinants related to the use of prescription drugs and self-medication
Provedor de dados: 60
Autores: Araújo,Danielle Dayse; Leal,Marineide Marinho; Santos,Eliane Jucielly Vasconcelos; Leal,Leila Bastos.
The use of drugs during pregnancy still represents a challenge for medicine, since the majority of drugs cross the placental barrier with a potential to cause several congenital problems to the fetus, and most of them have not been clinically tested in pregnant patients. At the same time, the medicalization phenomenon, self-medication, and lack of patient information about the misuse of medicines are additional problems. Thus, the aim of this study was to evaluate the pattern of medicine consumption in high-risk pregnancies and the determinants related to this consumption pattern. In order to do so, a cross-sectional descriptive study was performed with puerperal women who had a history of high-risk pregnancy. Statistically significant associations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/adverse effects; Pregnancy/medicines use; Self-medication; Health education.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300010

Stability indicating method development and validation for simultaneous estimation of atorvastatin calcium and celecoxib in bulk and niosomal formulation by RP-HPLC
Provedor de dados: 60
Autores: Jadhav,Priyanka S.; Jamkar,Priti M.; Avachat,Amelia M.
The present work describes development and validation of a specific, sensitive, precise and stability-indicating high-performance liquid chromatographic method of analysis of atorvastatin calcium and celecoxib, both as a bulk drug and in niosomal formulation. The analysis has been performed by using Cosmosil-C18 column (4.6 mm´250 mm, 5 m) at 25 °C using acetonitrile: ammonium acetate buffer pH 5.0: methanol (50:25:25 v/v/v) as mobile phase. The detection was carried out at 277nm with a flow rate of 1.0mL/min. The retention times of Atorvastatin calcium and Celecoxib were 6.195 and 3.989min, respectively. The method was validated according to ICH guidelines, for specificity, precision, linearity, accuracy and robustness. Atorvastatin calcium and Celecoxib...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atorvastatin calcium/determination; Celecoxib/determination; High performance liquid chromatography/quality control; Niosomal formulation/quality control; Pharmaceutical formulations/quality control.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300653

Preparation and evaluation of 2-methoxyestradiol-loaded pH-sensitive liposomes
Provedor de dados: 60
Autores: He,Suna; Wang,Bowen; Zhang,Runfang; Zhou,Huanhuan; Yang,Qian.
The development and clinical application of 2-methoxyestradiol (2-ME) as a new type of antitumor drug are limited due to its poor solubility, rapid metabolism in vivo, and large oral dosage. 2-ME-loaded pH-sensitive liposomes (2-ME-PSLs) was prepared containing the lipids, Lipoid E-80 (E-80), cholesteryl hemisuccinate (CHEMS), and cholesterol (CHOL) via thin-film ultrasonic dispersion. First, preparation conditions of 2-ME-PSLs were optimized by orthogonal test. Then 2-ME-PSL was characterized, and the release behavior and stability of 2-ME-PSL in vitro were evaluated. The optimal preparation conditions for 2-ME-PSLs were as follows: 2-ME : E-80+CHEMS 1:15; CHOL : E-80+CHEMS 1:5; ultrasonication time 20 minutes. The mean particle size, PDI, zeta potential,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-Methoxyestradiol; PH sensitive liposomes; In vitro release; Stability.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100544

Level of patient information on antibiotic use
Provedor de dados: 60
Autores: Fiol,Fernando de Sá Del; Barberato-Filho,Silvio; Lopes,Luciane Cruz; Toledo,Maria Inês de.
Establishing an effective antimicrobial therapeutic requires choosing the appropriate antibiotic and ensuring compliance with times and doses prescribed. Problems concerning early abandonment and unfinished prescriptions due to lack of information, as well as more resistant microorganisms, have been identified as the major factors responsible for therapeutic failure in bacterial infections. This study aimed to determine the level of information among antibiotic users undergoing treatment with these drugs, and habits related to other therapies. A survey was applied to 403 users and identified great difficulties in understanding prescriptions, especially among users with no formal schooling who understood only 21 to 28% of the prescribed instructions. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial therapeutic; Antimicrobial resistance; Antibiotics/rational use.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300006

Retrospective cost-utility and budget impact assessments of Hypericum perforatum in contrast with Fluoxetine treatment for depression in Karachi, Pakistan
Provedor de dados: 60
Autores: Ahmad,Syed Muzzammil; Masroor,Darakhshan; Azhar,Iqbal; Ahmed,Nadia.
In this study we have compared two different types of therapies i.e. herbal and allopathic system of therapies for Depression and studied them from the social perspectives. The Hypericum perforatum is compared with Fluoxetine [HCL] in terms of cost-utility and financial savings thereby evaluating its influence on annual expenditure of depressive patients that were randomly selected from 178 union councils of the city of Karachi, Pakistan. For both system of therapies a total of 356 patients were selected by stratified random sampling. Taking frequency of depression as ‘1’ annually with discount rate at 3% for calculating the burden-of-illness in terms of disability-adjusted-life-years. The cost-utility and the budget-impact assessments were carried out to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypericum perforatum; Fluoxetine; Burden of illness; Disability-Adjusted-Life-Years; Incremental-Cost-Effectiveness-Ratio.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100581

Pharmacist professionals in the prevention of drug abuse: updating roles, and opportunities
Provedor de dados: 60
Autores: Wagner,Gabriela Arantes; Andrade,Arthur Guerra de.
The objective of this paper was to prepare and provide resources to pharmacists and other healthcare professionals, enabling them to carry out a critical analysis on drug abuse, acquiring knowledge in several areas that effectively contribute to their personal development in this professional field. Professionals play a crucial role in the reduction and prevention of substances abuse, since they are able to advise patient about illicit drugs, psychotropic medicines and alcohol abuse. There is an urgent need to specialize pharmacists to act in the national public health service and contribute to actions aimed at the surrounding community.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical professional; Drugs/abuse; Drugs/dependence; Public health.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100003

Carbon nanotubes as a novel drug delivery system for anticancer therapy: a review
Provedor de dados: 60
Autores: Kushwaha,Swatantra Kumar Singh; Ghoshal,Saurav; Rai,Awani Kumar; Singh,Satyawan.
Carbon nanotubes (CNTs) were discovered in 1991 and shown to have certain unique physicochemical properties, attracting considerable interest in their application in various fields including drug delivery. The unique properties of CNTs such as ease of cellular uptake, high drug loading, thermal ablation, among others, render them useful for cancer therapy. Cancer is one of the most challenging diseases of modern times because its therapy involves distinguishing normal healthy cells from affected cells. Here, CNTs play a major role because phenomena such as EPR, allow CNTs to distinguish normal cells from affected ones, the Holy Grail in cancer therapy. Considerable work has been done on CNTs as drug delivery systems over the last two decades. However,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbon nanotubes/properties; Carbon nanotubes/use; Carbon nanotubes/drugs delivery; Single-Walled Carbon Nanotube; Multiwalled Carbon Nanotube; Anticancer drugs/delivery; Cancer/therapy; Drugs/delivery.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400002

Physical profile of counterfeit tablets Viagra® and Cialis®
Provedor de dados: 60
Autores: Ortiz,Rafael Scorsatto; Mariotti,Kristiane de Cássia; Limberger,Renata Pereira; Mayorga,Paulo.
The profile of tablets containing an active pharmacological ingredient can be obtained using different sets of properties, including physical and chemical aspects. The first measurements carried out on tablets are the physical characteristics also called post-tabletting batch (post-TB) characteristics. These data may be valuable to assist in the detection of pharmaceutical product forgery and may also be used in a forensic intelligence perspective when inserted into databases. This work is focused on the physical characteristics of Cialis® and Viagra® tablets seized by the Brazilian Federal Police in the Rio Grande do Sul state. Using the F-test (ANOVA), all samples of counterfeit Viagra® (n = 28) and Cialis® (n = 40) were well distinguished from authentic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Counterfeit drugs; Tablets/physical characteristics; Viagra®; Cialis®.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300016

Influence of sonication and in vitro evaluation of nifedipine self-nanoemulsifying drug delivery system
Provedor de dados: 60
Autores: Eid,Ahmad M; Elmarzugi,Nagib A; Jaradat,Nidal A.
In order to develop a self-nanoemulsifying system, three components, olive oil, Tween 80, and Capmul, were used to construct a ternary phase diagram that helped to find the optimum formulation, which was loaded with nifedipine. The effect of sonication on drug loading was also evaluated. After that, measurement of the droplet size, size distribution, zeta potential, and scanning electron microscopy were conducted for evaluation and characterisation of the formulations. The phase diagram of four formulations showed nanosizes below 200 nm; however, only one was selected to be loaded with nifedipine. The selected formulation had the lowest droplet size of 98 nm and size distribution 0.192, and was composed of 48% Tween 80, 32% Capmul, and 20% olive oil. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nifedipine; Self-nanoemulsifying drug delivery system; Sonication; SEM.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100586

Review of the efficacy and safety of over-the-counter medicine
Provedor de dados: 60
Autores: Halila,Gerusa Clazer; Czepula,Alexandra Ingrid dos Santos; Otuki,Michel Fleith; Correr,Cassyano Januário.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nonprescription medicines; Medicines/safety use; Self-medication; Evidence-based health.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200403

An in silico investigation of phytochemicals as potential inhibitors against non-structural protein 1 from dengue virus 4
Provedor de dados: 60
Autores: Qaddir,Iqra; Majeed,Arshia; Hussain,Waqar; Mahmood,Sajid; Rasool,Nouman.
Dengue fever has emerged as a big threat to human health since the last decade owing to high morbidity with considerable mortalities. The proposed study aims at the in silico investigation of the inhibitory action against DENV4-NS1 of phytochemicals from two local medicinal plants of Pakistan. Non-Structural Protein 1 of Dengue Virus 4 (DENV4-NS1) is known to be involved in the replication and maturation of viron in the host cells. A total of 129 phytochemicals (50 from Tanacetum parthenium and 79 from Silybum marianum) were selected for this study. The tertiary structure of DENV4-NS1 was predicted based on homology modelling using Modeller 9.18 and the structural stability was evaluated using molecular dynamics simulations. Absorption, distribution,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DENV4-NS1; Phytochemicals; Molecular Docking; Molecular Dynamics Simulations; Druglikeness.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100593

Rational use of antioxidants in solid oral pharmaceutical preparations
Provedor de dados: 60
Autores: Celestino,Maísa Teodoro; Magalhães,Uiaran de Oliveira; Fraga,Aline Guerra Manssour; Carmo,Flávia Almada do; Lione,Viviane; Castro,Helena Carla; Sousa,Valeria Pereira de; Rodrigues,Carlos Rangel; Cabral,Lucio Mendes.
Antioxidants are currently used as efficient excipients that delay or inhibit the oxidation process of molecules. Excipients are often associated with adverse reactions. Stability studies can guide the search for solutions that minimize or delay the processes of degradation. The ability to predict oxidation reactions in different drugs is important. Methods: This study was conducted to assess the rational use of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), sodium metabisulfite (SMB), propyl gallate (PG) and cysteine (CYS) in tablet formulations of simvastatin and ketoconazole. These antioxidants were evaluated according to stability parameters and the relationship between efficiency of the antioxidant and chemical structure of the drugs....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants/rational use; Excipients/evaluation; Butylated hydroxyanisole/rational use; Butylated hydroxytoluene/rational use; Sodium metabisulfite/rational use; Propyl gallate/rational use; Cysteine/rational use; Simvastatin/tablets; Simvastatin/evaluation; Ketoconazole/tablets; Ketoconazole/evaluation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300007

Noncompliance with drug therapy of glaucoma: a review about intervening factors
Provedor de dados: 60
Autores: Castro,Aldemar Nemésio Brandão Vilela de; Mesquita,Wander Araújo.
Glaucoma is defined as an optic neuropathy, characterized for loss of visual field and injury of the optic nerve, being considered as the second cause of blindness in the world, which could be prevented by the use of antiglaucoma eyedrops. The lack of adhesion of the patient to the drug treatment can culminate with loss of the vision. The objective was to revise possible literature data regarding intervening factors for noncompliance and explain estimated rates of noncompliance. A systematic review about the subject was carried out in the period of January to June of 2006. Articles had been searched in two data bases, in the National Library of Medicine (PUBMED) and in the Literature Latin American and Caribbean Health Sciences (LILACS) using the following...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glaucoma/drug treatment; Drug treatment/adhesion; Eyedrops/use; Eyedrops/evaluation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300010

Therapeutic adherence in outpatients with heart failure registered with a community pharmacy
Provedor de dados: 60
Autores: Despaigne,Rosario Megret; Sánchez,Carlos Manuel Dutok; Sarfraz,Alam.
A transverse descriptive study was carried out, according to the classification of therapeutic compliance, to evaluate adherence in 250 patients with a diagnosis of Heart Failure, registered with the health department of the municipality of Santiago de Cuba in 2009. The sample characterization was studied, with an assessment of adherence level and possible associated factors for sex, age and toxic habits. As an instrument for the work, data extraction was scheduled and the interview was carried out at patients' homes; the results were expressed in percentage and level of influence for associated factors. This was determined using the chi-square test. In the investigated population, adherence was greater for females, for age group 67-82 years, and toxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Treatment adherence; Heart failure/pharmacological treatment; Community pharmacy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100007

Protective effect of citrus lemon on inflammation and adipokine levels in acrylamide-induced oxidative stress in rats
Provedor de dados: 60
Autores: Haidari,Fatemeh; Mohammadshahi,Majid; Zarei,Mehdi; Fathi,Mojdeh.
The high exposure to acrylamide (AA) due to smoking and increased consumption of processed and fast foods in recent years, has become one of the health threatening problems. This study examined the effect of lemon juice on inflammation and adipokines in acrylamide-induced oxidative stress in rats. Forty animals were divided into five groups. Toxicity was induced by AA (35mg/kg) for two weeks in all groups except normal control group. After that, lemon juice in three doses was administrated to treatment groups for 4 weeks. Serum levels of adipokines and inflammatory parameters and both serum and liver levels of oxidative stress parameters were measured. The results showed groups were received AA had significant higher levels of malondialdehyde, tumor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus lemon; Acrylamide; Inflammation; Leptin; Adiponectin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100606

Determination of compatibility and stability of haloperidol and morphine mixtures used in palliative care
Provedor de dados: 60
Autores: María,Espinosa Bosch; Fuensanta,Sánchez Rojas; Catalina,Bosch Ojeda.
Abstract With the aim of controlling various symptoms, possible to use mixtures of different drugs within infusion devices. This should take into account the compatibility of the mixture. Factors influence the compatibility and stability of the mixtures are: drug type, concentration, solvent, temperature and light. When evaluating the compatibility of the mixtures for infusion for subcutaneous via is important to consider infusion devices used and the conditions of light and temperature should simulate as far as possible the conditions in practice assistance. There are diverse studies that analyze the compatibility of drug mixtures, but there are still many possible combinations of drugs for which evidence is not available. The objective of this work is to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haloperidol/stability/compatibility; Morphine/stability/compatibility; Mixtures; Palliative care.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200616

Evaluation of demands of inclusion, exclusion and alteration of technologies in the Brazilian Health System submitted to the National Committee on Technology Incorporation
Provedor de dados: 60
Autores: Nunes,Luciana Macatrão Nogueira; Fonteles,Marta Maria de França; Passos,Ana Cláudia Brito; Arrais,Paulo Sérgio Dourado.
ABSTRACT The steady increase in the number of health technologies and advances in associated research generate management challenges in the choice of technologies to be made available to the public. From 2011, the process of inclusion, exclusion and alteration of technologies to the Brazilian Unified Health System (SUS) has undergone major transformations, such as the creation of the National Committee on Technology Incorporation (CONITEC). This study analyzed the decisions of CONITEC since its inception until July 2015. The analyzed variables were: type of technology, reason for application, name of the technology, indication, claimant and CONITEC decision status. We found that the Government was the main claimant, accounting for 58.1% of the 420 cases....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Unified Health System/evaluation; Brazilian Unified Health System/technology; Health Technology Assessment; Health Technology; National Committee on Technology Incorporation/Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200625

Antihypoxic activities of Crataegus pentaegyn and Crataegus microphylla fruits-an in vivo assay
Provedor de dados: 60
Autores: Ebrahimzadeh,Mohammad Ali; Khalili,Masoumeh; Jafari,Negar; Zareh,Giti; Farzin,Davood; Amin,Gholamreza.
ABSTRACT The aim of this study was to evaluate anti-hypoxia activity of polyphenolic extracts of Crataegus microphylla and Crataegus pentaegyn on mice. Three experimental models of hypoxia were considered, including asphyctic hypoxia, haemic hypoxia, and circulatory hypoxia. Polyphenolic extract of both plants exhibited significant anti-hypoxic activity and prolonged animal survival time. Anti-hypoia activity of C. pentaegyn was found to be superior to that of C. microphylla in circulatory and asphyctic hypoxia. Antihypoxic activity of these extracts may be attributed to their phenolic compounds.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crataegus pentaegyna; Crataegus microphylla; Antihypoxia/evaluation; Circulatory hypoxia.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200615

Self-emulsifying therapeutic system: a potential approach for delivery of lipophilic drugs
Provedor de dados: 60
Autores: Wadhwa,Jyoti; Nair,Anroop; Kumria,Rachna.
Self-emulsifying therapeutic system (SETs) provide an effective and intelligent solution to the various issues related to the formulation of hydrophobic drugs with limited solubility in gastrointestinal fluid. Although the potential utility of SETs is well known, only in recent years has a mechanistic understanding of the impact of these systems on drug disposition emerged. These in situ emulsion-forming systems have a high stability when incorporated in various dosage forms. SETs are being looked upon as systems which can overcome the problems associated with delivery of poorly water soluble drugs. An in-depth knowledge about lipids and surfactants that can contribute to these systems, criterion for their selection and the proportion in which they can be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid based formulations; Self-emulsifying therapeutic system; Self-emulsification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300003

Unsatisfactory glycemic control in type 2 Diabetes mellitus patients: predictive factors and negative clinical outcomes with the use of antidiabetic drugs
Provedor de dados: 60
Autores: Marczynski,Maria Aparecida; Cortellazzi,Karine Laura; Barberato-Filho,Sílvio; Motta,Rogério Heládio Lopes; Vieira,Alexandre Eduardo Franzin; Quilici,Maria Teresa Verrone; Bergamaschi,Cristiane de Cássia.
ABSTRACT Many factors can interfere with glycemic control in patients with type 2 diabetes mellitus and this patient group has an increased risk of experiencing drug-related negative outcomes (DNO). The aim of this study was to identify the predictive factors of unsatisfactory glycemic control and DNO in this group of patients. A cross-sectional study was conducted collecting data from prescriptions and interviews with 100 patients of the Endocrinology Clinic of the Hospital Complex of Sorocaba, São Paulo, Brazil. Variables associated with unsatisfactory glycemic control were determined and the DNO associated with antidiabetic drugs identified. Age (&lt;65 years) (OR=4.09), family history of diabetes (OR=3.24), use of combined therapy to treat diabetes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 Diabetes mellitus; Type 2 Diabetes mellitus/study/drugs use; Pharmaceutical care; Drug-related negative outcomes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400801

A novel validated UPLC method for quantitation of lopinavir and ritonavir in bulk drug and pharmaceutical formulation with its impurities
Provedor de dados: 60
Autores: Killi,Gnana Deepika; Maddinapudi,Rama Krishna; Dinakaran,Sathis Kumar; Harani,Avasarala.
A simple gradient Ultra Performance liquid chromatographic method (UPLC) was developed for determination of lopinavir and ritonavir from its related impurities and assay for the ﬁrst time. This method involves the use of a C18 (Acquity UPLC BEH C18, 50 × 2.1 mm, 1.7 µm) column thermostated at 30 oC using triethylamine (pH 2.2): 0.1% H3PO4 in acetonitrile and methanol (85:15) as mobile phase in gradient elution mode. A Photo Diode Array (PDA) detector set at 215 nm was used for detection with flow rate 0.4 mL/min. This method was validated over the range of limit of quantitation (LOQ) to 50 to 150% of impurity speciﬁcation limit and of working concentration for assay. The developed method was validated for linearity, range, precision, accuracy and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lopinavir/determination; Ritonavir/determination; Ultra Performance liquid chromatography/qualitative analysis; Ultra Performance liquid chromatography/quantitative analysis; Bulk drugs/qualitative analysis; Pharmaceutical formulations/qualitative analysis; Pharmaceutical formulations/impurity profile; Bulk drugs/impurity profile.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200301

Medication-related inpatient falls: a critical review
Provedor de dados: 60
Autores: Ribeiro,Tatiane Bomfim; Melo,Daniela Oliveira de; Maia,Flávia de Oliveira Motta; Ribeiro,Eliane.
Abstract Falls are the second leading cause of accidental and unintentional injury deaths worldwide. Inpatient falls in hospital settings are likely to prolong the length of stay of patients in nearly 6.3 days, leading to increased hospitalization costs. The causes of fall incidents in healthcare facilities are multifactorial in nature and certain medications use could be associated with these incidents. This review seeks to critically evaluate the available literature regarding the relationship between inpatient falls and medication use. A comprehensive search was performed on MEDLINE, EMBASE and Lilacs with no time restriction. The search was filtered using English, Spanish or Portuguese languages. Our study evaluated medication use and inpatients falls...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Accidental Fall/hospitals; Therapeutic Uses; Inpatients; Central Nervous System Agents.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100404

Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest)
Provedor de dados: 60
Autores: Vilar,Juliana Brandstetter; Andrade,Laryssa Silva de; Leite,Kaio Ramos; Ferreira,Heleno Dias; Chen,Lee Chen.
Curatella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS inductest). To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the extract, Escherichia coli WP2s(λ) cultures were diluted in M9 buffer, inoculated into LB plates, and incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ) was treated with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain (RJF013), LB(1/2)(malt/amp), seeded into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curatella americana; Lixeira; Cytotoxicity; Genotoxicity; Lysogenic induction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300015

Beta-glucan attenuates cerebral ischemia/reperfusion-induced neuronal injury in a C57BL/J6 mouse model
Provedor de dados: 60
Autores: Kaya,Kürşat; Çiftçi,Osman; Öztanır,Mustafa Namık; Taşlıdere,Elif; Türkmen,Neşe Başak.
Beta-glucans (βg), that have many useful effects on human health, are natural polysaccharides. Our aim in this study was to determine useful effect of βg against oxidative and neuronal damage caused by global cerebral ischemia/reperfusion (IR) in stroke imitated mice via surgical operation. A total of 40 mice divided into four equal groups randomly. The group 1 (sham operated) was kept as control. Bilateral carotid arteries of subjects in group 2 (I/R) and group 4 (I/ R + βg) were clipped for 15 min, and the mice in group 4 (I/R + βg) were treated with βg (50 mg/kg/day), while the mice in group 2 (I/R) were treated with only vehicle for 10 days. The mice of group 3 (βg) were treated with βg for 10 days without carotid occlusion. Global cerebral I/R...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Global cerebral I/R; Oxidative stress; Neuronal damage; Beta-glucan; C57BL/J6 mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100570

Beta-glucan prevents toxic effects of 2,3,7,8-TCDD in terms of oxidative and histopathological damage in heart tissue of rats
Provedor de dados: 60
Autores: Ciftci,Osman; Duman,Ahmet Sefa; Turkmen,Neşe Basak; Taslıdere,Aslı.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a widespread environmental contaminant which causes severe toxic effects. Despite there is some suggestion concerning with TCDD induced cardiotoxicity such as formation of free radicals, the main mechanism has not been entirely explained. Beta-glucan is known as strong antioxidant matter and can scavenge free radicals. Therefore this study aimed to investigate the protective effects of beta-glucan against TCDD induced cardiotoxicity in rats. In this study, 2-3 months of age and 190-250 g in weight 32 rats were randomly divided into four equal groups (n=8 for each group). Group 1 was control; Group 2 was TCDD group (2 µg/kg/week); group 3 was the beta-glucan group(50 mg/kg/day), and group 4 was TCDD and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta-glucan/protective effects 2; 3; 7; 8-Tetrachlorodibenzo-p-dioxin (TCDD) Cardiotoxicity Rats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300623

Prevalence of hemoglobinopathies in school children: the importance of using confirmatory methods
Provedor de dados: 60
Autores: Tavares,Cristiane Fernandes de Freitas; Guimarães,Jacqueline da Silva; Souza,Ana Maria de.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemoglobinopathy; Hemoglobin electrophoresis; High performance liquid chromatography/quantitative analysis; Polymerase chain reaction.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200361

Chemical constituents and pharmacological profile of Gunnera manicata L. extracts
Provedor de dados: 60
Autores: Mariotti,Kristiane de Cássia; Schuh,Roselena Silvestri; Nunes,Jéssica de Matos; Salamoni,Sabrina Pinto; Meirelles,Gabriela; Barreto,Fabiano; Poser,Gilsane Lino Von; Singer,Rodrigo Bustos; Dallegrave,Eliane; Sand,Sueli Teresinha Van Der; Limberger,Renata Pereira.
Gunnera perpensa L. (Gunneraceae) is a native South African plant widely used in traditional medicine as an antibacterial and antifungal. In southern Brazil there is the native species called Gunnera manicata L. that also belongs to the Gunneraceae. Nevertheless, there is no information about chemical and pharmacological properties of South American Gunnera species. Therefore this study aimed at assessing the phytochemical and pharmacological profiles of aqueous and methanol Brazilian G. manicata extracts. The results showed that antimicrobial activity in an agar diffusion assay was effective against Staphylococcus aureus and Candida albicans . Phenolic compounds were investigated by liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gunnera manicata L./phytochemistry; Gunnera manicata L./pharmacognosy; Gunneraceae /pharmacognosy; Gunnera manicata L./extracts/antimicrobial effect; Gunnera manicata L./extracts/antioxidant activity; Gunnera manicata L./extracts/antimicrobial assay.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100015

Analgesia: Methods and protocols
Provedor de dados: 60
Autores: Fortes,Zuleica Bruno.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400031

Analysis of extemporaneous oral liquid from commercially available drugs in hospital
Provedor de dados: 60
Autores: Benzi,Jhohann Richard de Lima; Mastroianni,Patrícia De Carvalho.
ABSTRACT The objective of this study was to identify drugs that received dose adjustments (DA) and pharmaceutical alternatives (PA) that avoid DA, and calculate the economic percentage of this replacement. A descriptive, observational and cross-sectional study was performed in a second level hospital. The pharmacy and nursing services was accompanied to identify the drugs that received DA and the compounding techniques. After identifying all the drugs that received DA, was identified in the Brazilian market the corresponding pharmaceutical alternative, with the Drugs Price List of Brazilian Health Regulatory Agency. For those drugs that was not available any PA, was performed a research of studies that describe compounding techniques in international...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical preparations/hospitals; Patient safety; Pharmacoeconomics; Drugs/descritive study/ Off-Label Use.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300517

Aluminium and calcium lake pigments of Lac natural dye
Provedor de dados: 60
Autores: Jimtaisong,Ampa.
This work presents the physicochemical properties of the prepared lac lakes. The study revealed the stability, cytotoxicity and use of lac lake pigment as a cosmetic colorant. SEM, XRD and DSC were used to study the characteristics of the lac lakes.. The color stability of lac dye and its lakes was investigated at ambient temperature and at 45 °C, in sunlight,fluorescent light and darkness, for 3 months. Cytotoxicity on human dermal skin fibroblast cell lines was tested using MTT assay and the use of the lakes as a colorant in lip color product was studied. Different metal salts resulted in lac lakes of different shades and appearance. SEM, XRD and DSC results indicated that the morphology and structure of lac dye were changed after reacting with metal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laccaic acids; Natural lac dye; Lake pigment; Cosmetics.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100551

Synergy: an old wisdom, a new paradigm for pharmacotherapy
Provedor de dados: 60
Autores: Biavatti,Maique Weber.
Synergy is one of the governing principles of the natural world, and is one of the reasons for the increasing complexity of the evolutionary process. Broadly speaking, it relates to the cooperative effects produced by the interaction between various forces, elements, parts or individuals in a given context. Since the last century, some clinical studies have described the increased efficiency of a combination of drugs. To study the effects of the drug interactions, the most commonly cited model is that of Loewe, and the classical graphical representation is the iso-effect curve (isobologram). Plants contain an enormous diversity of specialized micromolecules, therefore there is a high likelihood of interactions between them. Through recent synergy studies,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural extracts; Synergy; Phytotherapeutics.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300002

Hepatic protective effect of grape seed proanthocyanidin extract against Gleevec-induced apoptosis, liver Injury and Ki67 alterations in rats
Provedor de dados: 60
Autores: Al-Rasheed,Nawal Mohammed; El-Masry,Thanaa Ahmed; Tousson,Ehab; Hassan,Hanan Mohammad; Al-Ghadeer,Areej.
ABSTRACT Gleevec (imatinib) is an antineoplastic chemotherapeutic agent used in the treatment of many types of cancer. The current study was conducted to examine the possible modifying effects of grape seeds proanthocyandins extract (GSPE) against apoptosis, liver injury and Ki67 alterations induced by Gleevec in male rats. 40 male albino rats were equally divided into four groups (First and second groups were control and GSPE groups; third group was Gleevec group and fourth group was treated with Gleevec and GSPE). Gleevec induced elevations in P53 and depletion of Bcl2 levels in liver tissues were compared with the control group. Liver sections in rats treated with Gleevec exhibited marked cellular infiltrations, vacuolar degeneration hepatocytes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemotherapy; Gleevec/effects; Gleevec/Imatinib; Proanthocyandins; Liver; Apoptosis; Proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200612

The influence of pH, polyethylene glycol and polyacrylic acid on the stability of stem bromelain
Provedor de dados: 60
Autores: Novaes,Letícia Celia de Lencastre; Jozala,Angela Faustino; Mazzola,Priscila Gava; Júnior,Adalberto Pessoa.
Enzyme stability is critical in biotechnology, pharmaceutical and cosmetic industries. Investigations on this subject have drawn attention because of its practical application. Bromelain is a thiol-endopeptidase, obtained from pineapple (Ananas comosus), known for its clinical and therapeutic applications, particularly to selective burn debridement and improvement of antibiotic action and anti-inflammatory activities. To date, the use of bromelain in pharmacological or industrial applications is limited, due to commercial availability, costs, and sensitivity to pH and temperature. Therefore, a better understanding of enzyme stability would be of great interest. The aim of this study was to evaluate bromelain activity and stability in several pH (2.0 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromelain/pH stability; Polyethylene glycol/enzyme stability; Polyacrylic acid/enzyme stability; Enzyme/stability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200371

Pharmaceutical assistance within the SUS: the experience of students in Rural Internship from a Pharmacy Course
Provedor de dados: 60
Autores: Saturnino,Luciana Tarbes Mattana; Luz,Zélia Profeta; Perini,Edson; Modena,Celina Maria.
The 2002 Brazilian Curricular Lines established a new curriculum for Pharmacy Programs, including amplified information about the Unified Health System (SUS). Following this, some Colleges have implemented a Rural Internship (RI) discipline, as a way to promote: a) adequate information on the SUS, and b) students' interaction with pharmaceutical assistance. In this study we analyzed the perceptions of students enrolled in the Rural Internship program of the undergraduate Pharmacy Program at the Federal University of Minas Gerais. Eight students participated in this study and their perceptions and ideas were obtained by focus groups, both before and after the RI. This information was analyzed by content analysis. The students had a fragmented, distorted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical assistance; Pharmacy/education; Unified Health System; Rural Internship.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400028

Validation of high performance liquid chromatographic and spectrophotometric methods for the determination of the antiparkinson agent pramipexole dihydrochloride monohydrate in pharmaceutical products
Provedor de dados: 60
Autores: Sevim,Serpil; Erk,Nevin.
abstract The antiparkinson agent pramipexole dihydrochloride monohydrate was quantified in pharmaceutical products by high performance liquid chromatography (HPLC) and derivative spectrophotometry. The first method was based on HPLC using tamsulosin HCl as an internal standard. In this method, chromatographic separation was achieved using a LiChrospher 60 RP column at 25°C, with a flow rate of 1.0 mL/min at 263 nm. The eluent comprised 0.01 mol/L ammonium acetate (pH 4.4) and acetonitrile (35:65 by volume). The linearity range was found to be 10.0-30.0 µg/mL with a mean recovery of 100.5 ± 1.10. The limit of detection (8 ng/mL) and limit of quantification (50 ng/mL) were calculated. In the second method, the first derivative spectrophotometric technique...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiparkinson agent/determinação; Pramipexole dihydrochloride monohydrate; Pharmaceutical product/quality control; High performance liquid chromatography/quantitative analysis; Derivative spectrophotometry/quantitative analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400879

Vitamin K: content in food consumed in São Paulo, Brazil
Provedor de dados: 60
Autores: Faria,Simone Aparecida dos Santos Conceição; Arruda,Vanilda Aparecida Soares de; Araújo,Elias da Silva; Penteado,Marilene De Vuono Camargo.
ABSTRACT Recent research on Vitamin K has shown its importance in maintaining vascular and bone health. Brazilian food composition tables do not show phylloquinone content in national foods. These data are needed to obtain more reliable results in nutritional status assessment studies of individuals in relation to this vitamin as studies have shown a geographical influence in food phylloquinone content. This study aims to determine phylloquinone (Vitamin K1) levels in its most important source: dark green leaved vegetables. Several varieties of vegetables were purchased directly from CEAGESP (General Warehouse Company of São Paulo) at different times. Phylloquinone was extracted using organic solvents and quantified by High Performance Liquid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylloquinone/determination/leaf vegetables; Food composition; High Performance Liquid Chromatography; Vitamin K/study/Brazil; Cooking/Loss.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200603

Preparation and characterization of cefuroxime axetil solid dispersions using poloxamer 188
Provedor de dados: 60
Autores: Sankari,Thaeer; Al-Hariri,Sahar.
The main objective of the present work was to enhance the solubility and dissolution rate of poorly water-soluble drug cefuroxime axetil (CA) by formulating it into solid dispersions (SDs) with water soluble carrier poloxamer 188. Different methods were employed to prepare the dispersion, such as: Solvent method (SM), Kneading method (KM), Melt evaporation method (MEM) and Physical mixture (PM) in different drug: carrier ratios 1:1, 1:2 and 1:3 (cefuroxime axetil: poloxamer 188). The physical mixture(s) and solid dispersion(s) were characterized for drug carrier interaction, drug content, solubility, dissolution rate, differential scanning calorimetry (DSC) and FT-IR study. The dissolution rate of the prepared solid dispersion systems was determined in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cefuroxime axetil/dissolution; Poloxamer 188; Solid dispersion.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400622

Internal consistency and interrater reliability of the Brazilian version of Martín-Bayarre-Grau (MBG) adherence scale
Provedor de dados: 60
Autores: Matta,Samara Ramalho; Azeredo,Thiago Botelho; Luiza,Vera Lucia.
ABSTRACT This paper aims to analyze the measurement equivalence aspects (internal consistency and interrater reliability) of a Brazilian version of Martín-Bayarre-Grau (MBG) adherence questionnaire as part of its cross-cultural adaptation. Item-total correlation and Cronbach's alpha coefficients were used as internal consistency estimates. Stability was evaluated through test and retest comparison and expressed through intraclass correlation coefficient (ICC) and kappa with quadratic weighting. ICC for the overall scale was 0.81, indicating an "almost perfect" agreement. However, some cases of "poor" and "slight" agreements were found while analyzing individual items. The translated version of the MBG questionnaire showed good homogeneity (alpha 0.78),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adherence to medication; Reproducibility of results; Questionnaires/study; Martín-Bayarre-Grau/study/aspects.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400795

Preparation, characterization and evaluation of the in vivo trypanocidal activity of ursolic acid-loaded solid dispersion with poloxamer 407 and sodium caprate
Provedor de dados: 60
Autores: Eloy,Josimar Oliveira; Saraiva,Juliana; Albuquerque,Sérgio de; Marchetti,Juliana Maldonado.
Ursolic acid is a promising candidate for treatment of Chagas disease; however it has low aqueous solubility and intestinal absorption, which are both limiting factors for bioavailability. Among the strategies to enhance the solubility and dissolution of lipophilic drugs, solid dispersions are growing in popularity. In this study, we employed a mixture of the surfactants poloxamer 407 with sodium caprate to produce a solid dispersion containing ursolic acid aimed at enhancing both drug dissolution and in vivo trypanocidal activity. Compared to the physical mixture, the solid dispersion presented higher bulk density and smaller particle size. Fourier Transform Infrared Spectroscopy results showed hydrogen bonding intermolecular interactions between drug and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ursolic acid; Chaga's Disease; Solid dispersions; Poloxamer 407; Sodium caprate.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100101

In vivo analgesic and anti-inflammatory effects of the essential oil from Tanacetum balsamita L.
Provedor de dados: 60
Autores: Sharif,Maryam; Najafizadeh,Parvaneh; Asgarpanah,Jinous; Mousavi,Zahra.
Tanacetum balsamita locally called Shahesparam is an aromatic plant that grows widely in Azerbaijan Province, Iran. Due to the widespread use of T. balsamita as a pain killer and relief of inflammatory based disorders in Iranian folk medicine and considering the high content of essential oil in T. balsamita aerial parts, we were prompted to investigate the anti-nociceptive and anti-inflammatory properties of T. balsamita essential oil (TBEO) for the first time. The carrageenan-induced Paw Edema was used for inflammation evaluation in rat, and hot-plate method was used for pain assessment in mice. Different doses of TBEO were administered. Morphine and Mefenamic acid were used as the standard drugs in anti-nociceptive and anti-inflammatory evaluation tests,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tanacetum balsamita; Essential oil; Analgesic; Antiinflammatory.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100556

Swertiamarin attenuates carbon tetrachloride (CCl4)-induced liver injury and inﬂammation in rats by regulating the TLR4 signaling pathway
Provedor de dados: 60
Autores: Wu,Tao; Zhang,Qianrui; Song,Hongping.
The aim of the present study is to illustrate the effects of swertiamarin (STM), a natural iridoid from herbal medicines, on hepatic inﬂammation induced by carbon tetrachloride (CCl4) in rats. Male Sprague Dawley rats were exposed to CCl4 with or without STM co-administration for 8 weeks. Our results revealed that STM administration (100 and 200 mg/kg b.w.) signiﬁcantly attenuated inflammation in livers of CCl4-treated rats. STM remarkably reduced the production of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), macrophage inflammatory protein-1a (MIP-1α), and monocyte chemotactic protein-1 (MCP-1) in liver tissue of CCl4-treated rats. In addition, STM treatment downregulated connective tissue growth factor (CTGF) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swertiamarin/effects; Carbon tetrachloride; Inﬂammation; TLR4; NF-κB.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400618

A study of the phytotoxic effects of the aerial parts of Senecio westermanii Dusén (Asteraceae) on Lactuca sativa L. and Allium cepa L. seeds
Provedor de dados: 60
Autores: Merino,Francis José Zortéa; Ribas,Daniele Felipe; Silva,Cristiane Bezerra da; Duarte,Ana Flávia Schvabe; Paula,Cristiane da Silva; Oliveira,Maislian de; Dias,Josiane de Fátima Gaspari; Miguel,Marilis Dallarmi; Miguel,Obdulio Gomes.
The objective of this study was to evaluate the effects of the ethanolic crude extracts and fractions of the species Senecio westermanii Dusén on Lactuca sativa L. (lettuce) and Allium cepa L. (onion) seeds. We assessed the germination, growth, root respiration and photosynthesis of the target species in Petri dishes (9.0 cm diameter) containing filter paper n°6. The study was conducted using 50 seeds per plate and held in 4 replicates per concentration of each sample. In the germination there was an inhibitory effect of fractions hexane (FH) and chloroform (FCl) at concentrations of 500 and 1000 µg/mL. There was a reduction in the radicle growth of lettuce by 14 to 24% and a reduction of hypocotilum by 14 to 28%. As for the radicle of the onion was up 74%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Senecio westermanii/extract/effects; Lactuca sativa; Allium cepa; Allelochemicals; Allelopathy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300606

Critical analysis of the regulations regarding the disposal of medication waste
Provedor de dados: 60
Autores: Bellan,Natalia; Pinto,Terezinha de Jesus Andreoli; Kaneko,Telma Mary; Moretto,Lauro Domingos; Santos Junior,Nelson dos.
Expired or unused medication at people's homes is normally disposed of in normal garbage, sewage system or, in certain cases, returned to the public health system. There is still no specific legislation regarding this leftover medication to regulate and orient the handling and correct disposal of medication waste. However, there is defined regulation regarding health services' solid waste. This article has the objective of discussing management models for the disposal of medication waste and the recommendations made by pertinent national and international legislation. By means of literature reviews, the management structure for medication waste of international legislation and the regulations regarding the environment, as well as the national legislation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medications/disposal; Medications/solid waste; Medications/disposal; Sanitary risk; Environmental contamination; National Solid Waste Policy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300018

Activity of antiretroviral drugs in human infections by opportunistic agents
Provedor de dados: 60
Autores: Demarchi,Izabel Galhardo; Cardozo,Daniela Maira; Aristides,Sandra Mara Alessi; Moliterno,Ricardo Alberto; Silveira,Thaís Gomes Verzignassi; Cardoso,Rosilene Fressatti; Bertolini,Dennis Armando; Svidzinski,Terezinha Inez Estivalet; Teixeira,Jorge Juarez Vieira; Lonardoni,Maria Valdrinez Campana.
Highly active antiretroviral therapy (HAART) is used in patients infected with HIV. This treatment has been shown to significantly decrease opportunist infections such as those caused by viruses, fungi and particularly, protozoa. The use of HAART in HIV-positive persons is associated with immune reconstitution as well as decreased prevalence of oral candidiasis and candidal carriage. Antiretroviral therapy benefits patients who are co-infected by the human immunodeficiency virus (HIV), human herpes virus 8 (HHV-8), Epstein-Barr virus, hepatitis B virus (HBV), parvovirus B19 and cytomegalovirus (CMV). HAART has also led to a significant reduction in the incidence, and the modification of characteristics, of bacteremia by etiological agents such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Highly active antiretroviral therapy; Antiretroviral drugs/activity; Opportunist infections/treatment.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100019

Physical and physicochemical stability evaluation of cosmetic formulations containing soybean extract fermented by Bifidobacterium animalis
Provedor de dados: 60
Autores: Vieira,Rafael Pinto; Fernandes,Alessandra Ribeiro; Kaneko,Telma Mary; Consiglieri,Vladi Olga; Pinto,Claudinéia Aparecida Sales de Oliveira; Pereira,Claudia Silva Cortez; Baby,André Rolim; Velasco,Maria Valéria Robles.
Peel off facial masks, based on polyvinyl alcohol (PVA), are formulations that, after application and drying, form an occlusive film over the face. After removing, they provide cleanness, tensor and moisturizing effects, removing dead cells, residues and other materials deposited on the stratum corneous. The soybean extract fermented by Bifidobacterium animalis has sugars, amino acids, peptides, proteins and free isoflavonoids in high concentrations, when compared to the unfermented extract, providing benefits to the cosmetic formulations like anti-aging effect, moisture, tensor action and emollience. The cosmetic bases of peel off facial masks, added with 5.0% w/w of fermented soybean extract, were submitted to Preliminary and Accelerated Stability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmetic formulations/physical and physicochemical stability; Facial mask peel off; Polyvinyl alcohol; Soybean extract; Bifidobacterium animalis/fermentation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300018

Influence of fermentation time on characteristics of sourdough bread
Provedor de dados: 60
Autores: Aplevicz,Krischina Singer; Ogliari,Paulo José; Sant'Anna,Ernani Sebastião.
Sourdough is used in the manufacture of numerous baked products. The microorganisms used in this preparation of sourdoughs included two strains from the Lactobacillus paracasei (1 and 2) and two strains from the Saccharomyces cerevisiae group (1 and 2). Samples of raw dough were analyzed for pH, titratable acidity and plate counts and samples of resulting bread were analyzed for pH, titratable acidity and specific volume. The samples were analyzed every 2 h, between 4 and 10 h of fermentation. After 10 hours of fermentation, the lowest values of pH were for dough with LC2 and bread with SC1. Titratable acidity values increased over time, with the highest levels of acidity were found in the dough and bread with yeasts. Lactic acid bacteria showed the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sourdough; Bread; Lactic acid bacteria; Yeast; Linear regression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200005

Caffeine increases Nr1i3 expression and potentiates the effects of its ligand, TCPOBOP, in mice liver
Provedor de dados: 60
Autores: Fukumasu,Heidge; Rochetti,Arina Lázaro; Latorre,Andreia Oliveira; Pires,Pedro Ratto Lisboa; Silva,Tereza Cristina; Dagli,Maria Lucia Zaidan.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caffeine/effects/experimental study Androstane constitutive receptor/caffeine effects 1 4-&lt; Italic&gt; Bis&lt; /italic&gt; -[2-(3 5-dichloropyridiloxy)]benzeno 3 3′ 5 5-tetracloro-1 4-&lt; Italic&gt; Bis&lt; /italic&gt; (pyridiloxy)benzene Cytochrome p450.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200295

Antihyperglycemic and neuroprotective effects of Wattakaka volubilis (L.f.) Stapf root against streptozotocin induced diabetes
Provedor de dados: 60
Autores: Haroon,Hajira Banu; Murali,Anita.
ABSTRACT Murva is an important drug in Ayurveda. Wattakaka volubilis is used as one of the botanical sources of Murva. The aim of this study is to evaluate the effect of the alcohol extract of W. volubilis root in streptoztocin (STZ) induced diabetes and diabetic neuropathy. Diabetes mellitus (DM) was induced by the administration of STZ (45 mg/kg, i.p). DM was induced within 72 h. Diabetic animals were treated with glimepiride (0.5 mg/kg) and ethyl alcohol extract 100 and 200 mg/kg for 21 d. After determining the changes in fasting serum glucose and lipid profile, animals were further treated for a period of 15 d to determine the protective effect of extract against diabetic neuropathy. All the alcohol extract treated animals, showed a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus/study; Diabetic neuropathies; Wattakaka volubilis (L.f.) Stapf/ root/induced diabetes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300413

Controlled release alginate-chitosan microspheres of tolmetin sodium prepared by internal gelation technique and characterized by response surface modeling
Provedor de dados: 60
Autores: Elsayed,Mahmoud M. A.
Tolmetin sodium (TS) is a powerful non-steroidal mitigating drug for the treatment of rheumatoid joint inflammation, osteoarthritis, and adolescent rheumatoid joint pain. In addition to its gastrointestinal (GIT) problems, TS has a short biological half-life (1 hr). In a trial to overcome these side effects and control the rate of (TS) release, chitosan coated alginate microspheres are recommended. A Box-Behnken experimental design was employed to produce controlled release microspheres of TS in the sodium alginate and chitosan copolymers (Alg-Ch) by emulsification internal gelation methodology. The effect of critical formulation variables namely, drug to polymer ratio (D:P ratio), speed of rotation and span 80% on drug encapsulation efficiency (% EE),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tolmetin Sodium; Controlled Release; Alginate; Chitosan; Response Surface.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100604

Evaluation of skin absorption of drugs from topical and transdermal formulations
Provedor de dados: 60
Autores: Ruela,André Luís Morais; Perissinato,Aline Gravinez; Lino,Mônica Esselin de Sousa; Mudrik,Paula Silva; Pereira,Gislaine Ribeiro.
ABSTRACT The skin barrier function has been attributed to the stratum corneum and represents a major challenge in clinical practice pertaining to cutaneous administration of drugs. Despite this, a large number of bioactive compounds have been successfully administered via cutaneous administration because of advances in the design of topical and transdermal formulations. In vitro and in vivo evaluations of these novel drug delivery systems are necessary to characterize their quality and efficacy. This review covers the most well-known methods for assessing the cutaneous absorption of drugs as an auxiliary tool for pharmaceutical formulation scientists in the design of drug delivery systems. In vitro methods as skin permeation assays using Franz-type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skin absorption/effects/study; Skin absorption/topical formulations; Skin absorption/transdermal formulations; Skin absorptions/enhancers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300527

Use of medicinal herbs by patients with severe asthma managed at a Referral Center
Provedor de dados: 60
Autores: Mega,Tacila Pires; Santos,Pablo de Moura; Souza-Machado,Adelmir; Noblat,Lúcia de Araújo Beisl Costa; Cruz,Álvaro Augusto.
Asthma is a chronic inflammatory disease of the airways that may lead to limitations in regular activities, to hospitalizations and a decrease in quality of life. Adherence to drug treatment is crucial for control of the disease. The use of medicinal herbs can reduce adherence to prescriptions, as the medication may be replaced by infusions or herbal products. The objective of this study was to evaluate the frequency of use of traditional herbal medicine among severe asthmatics in Salvador. Information on use of homemade remedies was obtained through application of a questionnaire during patient visits to a referral center. We also collected data on economic and social aspects as well as disease control. One hundred and forty-four (91,1%) out of one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma/adherence to drug treatment; Medicinal herbs/use.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300024

Particle size and morphological characterization of cosmetic emulsified systems by Optical Coherence Tomography (OCT)
Provedor de dados: 60
Autores: Prestes,Paula Souza; Peres,Daniela D'Almeida; Freitas,Anderson Zanardi de; Consiglieri,Vladi Olga; Kaneko,Telma Mary; Velasco,Maria Valéria Robles; Baby,André Rolim.
ABSTRACT The physicochemical attributes of emulsified systems are influenced by the characteristics of their internal phase droplets (concentration, size and morphology), which can be modified not only by the formulation components, but also by the analytical methodology employed. Thus, the aim of this work involved the physicochemical characterization of cosmetic emulsions obtained from different surfactants, as well as the introduction of the optical coherence tomography (OCT) as the analytical technique employed for the morphological characterization and particle size determination of the formulations. Three emulsions were prepared, differing at the type and concentration of the surfactant used, and their droplet sizes were evaluated through optical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmetic emulsions/physicochemical characterization; Surfactants; Optical coherence tomography/morphological characterization; Optical microscopy/morphological characterization; Laser diffraction/morphological characterization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200273

Erratum: v.45, n.4, 2009
Provedor de dados: 60
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100019

Elderly and drugs: risks and necessity of rational use
Provedor de dados: 60
Autores: Baldoni,André de Oliveira; Chequer,Farah Maria Drumond; Ferraz,Elisa Raquel Anastácio; Oliveira,Danielle Palma de; Pereira,Leonardo Régis Leira; Dorta,Daniel Junqueira.
In recent decades, the world has undergone a demographic transformation with a rapid growth of the elderly population, resulting in an increased demand for funds to maintain their health and drug consumption. Pharmacokinetic and pharmacodynamic changes occurring in the elderly can interfere directly in the adverse effects of drugs and increase the risk of intoxication. In addition, there are external factors interfering with the pharmacotherapy of the elderly, such as inappropriate use and the lack of access to information. Many therapeutic classes of drugs should be used with caution or avoided in the elderly population, such as anti-inflammatory and some anti-hypertensive drugs, diuretics and digitalis. If not managed carefully, these medicines can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly/inappropriate medicines; Medicines/consumption; Medicines/adverse reaction; Medicines/intoxication; Medicine/rational use; Medicine/inappropriate prescribing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400003

Influence of preparation procedures on the phenolic content, antioxidant and antidiabetic activities of green and black teas
Provedor de dados: 60
Autores: Almeida,Tânia Santos de; Araújo,Maria Eduarda Machado; Rodríguez,Lucía González; Júlio,Ana; Mendes,Beatriz Garcia; Santos,Rui Miguel Borges dos; Simões,José Artur Martinho.
The influence of common tea preparation procedures (temperature, infusion time, consumption time interval and tea bag/loose-leaf) and the type of water used, on the total phenolic content (TPC), the radical scavenging activity and the α-glucosidase inhibitory activity were assessed. Higher TPC and antioxidant activity were obtained when using lower mineralized waters. Tea bags also evidenced higher antioxidant activity than loose-leaf samples. Under the same conditions (90 ºC and five minutes of infusion time) green tea contains almost twice the quantity of polyphenols and the free radical scavenging ability of black tea. In the α-glucosidase assay all infusions were active (97-100 %). Furthermore, HPLC allowed to identify some of the polyphenols present...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Black tea; Green tea; Antioxidant activity; Α-Glucosidase activity; Water mineralization.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100529

Acute toxicity study of a polysaccharide based hydrogel from linseed for potential use in drug delivery system
Provedor de dados: 60
Autores: Haseeb,Muhammad Tahir; Bashir,Sajid; Hussain,Muhammad Ajaz; Ashraf,Muhammad Umer; Erum,Alia; Naeem-ul-Hassan,Muhammad.
ABSTRACT Linseed hydrogel (LSH) was evaluated by acute toxicity for its potential application in oral drug delivery design. White albino mice and rabbits were divided in four groups (I-IV) and different doses of LSH (1, 2 and 5 g/kg body weight) were given except to the control group (I) that was left untreated. Rabbits were monitored for eye irritation, acute dermal toxicity and primary dermal irritation, whereas, body weight, food and water consumption, hematology and clinical biochemistry, gross necropsy and histopathology of vital organs were scrutinized in mice. LSH was considered safe after eye irritation test as no adverse signs or symptoms were seen in the eye. In dermal toxicity and irritation study, skin of treated rabbits was found normal in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linseed hydrogel; Acute toxicity; Hematology; Clinical biochemistry; Polysaccharide.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200610

Pharmaceutical consultation as a tool to improve health outcomes for patients with type 2 diabetes
Provedor de dados: 60
Autores: Zubioli,Arnaldo; Silva,Maria Angélica Rafaini Covas Pereira da; Tasca,Raquel Soares; Curi,Rui; Bazotte,Roberto Barbosa.
This study develops and evaluates a pharmaceutical consultation program (PCP) to improve treatment for Type 2 diabetes patients (T2DP) and reduce risk factors for diabetic complications with possible application in other chronic diseases. We recruited T2DP receiving conventional medical treatment but with fasting glycemia &amp;gt;140mg/dl and/or glycated hemoglobin &amp;gt;7%. The PCP includes strategies obtained from Dader's method, the PWDT (Pharmacist's Workup of Drug Therapy method) model of pharmaceutical care, the SOAP (Subjective data, Objective data, Assessment, and Plan of care) method, and concepts based on a nursing care model. The PCP evaluated lifestyle, pharmacotherapy and monitoring it using laboratory tests, vital signs, and anthropometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care; Type 2 diabetes/treatment; Type 2 diabetes/pharmaceutical care; Pharmaceutical consultation; Glycated hemoglobin; Diabetes education; Pharmacy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100010

Formulation of a modified release metformin. HCl matrix tablet: influence of some hydrophilic polymers on release rate and in-vitro evaluation
Provedor de dados: 60
Autores: Rojas,John; González,Cesar; Rico,Carolina; Saez,Oswaldo.
Metformin hydrochloride is an antidiabetic agent which improves glucose tolerance in patients with type 2 diabetes and reduces basal plasma levels of glucose. In this study, a simplex centroid experimental design with 69 runs was used to select the best combination of some hydrophilic polymers that rendered a 24 h in-vitro release profile of metformin.HCl. The Korsmeyer-Peppas model was used to model the dissolution profiles since it presented the best fit to the experimental data. Further, a cubic model predicted the best formulation of metformin.HCl containing polyvinyl pyrrolidone, ethyl cellulose, hydroxypropyl methyl cellulose, carrageenan, sodium alginate, and gum arabic at 6.26, 68.7, 6.26, 6.26, 6.26 and 6.26 % levels, respectively. The validation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metformin/release profile; Metformin.HCl/modified release; Metformin.HCl/in-vitro evaluation; Hydrophilic polymers/influence; Hydrophilic polymers/drugs release; Korsmeyer-Peppas model/dissolution profiles.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300006

Does hospital admission provide an opportunity for improving pharmacotherapy among elderly inpatients?
Provedor de dados: 60
Autores: Melo,Daniela Oliveira de; Storpirtis,Sílvia; Ribeiro,Eliane.
ABSTRACT The purpose of the work was to assess the incidence of potential drug interactions (pDDI), major pDDI, and the use of potentially inappropriate medication (PIM) at hospital admission, during hospitalization, and at discharge to evaluate whether hospital admission provides an opportunity for improving pharmacotherapy in elderly patients at a University hospital that has a clinical pharmacist. A prospective cohort study was carried out using data from the medical records of patients admitted to an internal medicine ward. All admissions and prescriptions were monitored between March and August 2006. Micromedex(r) DrugReax(r) and Beers Criteria 2015 were used to identify pDDI, major pDDI, and PIMs, respectively. A comparison of admission and discharge...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Health Services/elderly patients; Internal Medicine/hospitals; Clinical Pharmacy; Drug interactions; Drug prescriptions.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300391

Copaiba oleoresin: evaluation of the presence of polycyclic aromatic hydrocarbons (PAHs)
Provedor de dados: 60
Autores: Silva,Wilson Gomes da; Cortesi,Nicoletta; Fusari,Paola.
The copaiba oleoresin extracted by perforating the tree-trunk found in the Amazonian forest has been used by the native indigenous communities to treat several diseases and also as fuel for lighting and for the motorboats plying the region's rivers. Currently, the oleoresin is mostly employed as a traditional remedy, mainly for the treatment of tonsillitis and as an anti-inflammatory and healing agent in oil and capsule forms, and is also used in several industry sectors. Due to its use in oral form, especially as a traditional remedy, an analysis of the presence of polycyclic aromatic hydrocarbons (PAHs) as contaminants in the oleoresin was performed. PAHs are substances formed by two or more benzoic rings and found in the atmosphere as a residue from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copaiba/oil; Copaiba/qualitative analysis; Polycyclic aromatic hydrocarbons; High performance liquid chromatography/qualitative analysis; Amazônia/traditional remedy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300025

Pharmacokinetics, safety and tolerability of L-3-n-butylphthalide tablet after single and multiple oral administrations in healthy Chinese volunteers
Provedor de dados: 60
Autores: Wang,Meng; Zhang,Quan-ying; Hua,Wen-yan; Huang,Ming; Zhou,Wen-jia; Lou,Kun; Peng,Yue-ying.
L-3-n-butylphthalide (L-NBP) is a naturally occurring antioxidant, which can be used for the treatment of acute ischemic stroke and vascular dementia. This study evaluated the safety, tolerability and pharmacokinetics of L-NBP tablets in healthy Chinese volunteers. This was a single-center, randomized, double-blind, placebo-controlled, single- and multiple-dose study. Subjects were assigned to receive a single dose of L-NBP tablet at either 80, 160, 320, or 480 mg (n=40), or multiple doses of 160 mg twice daily for 7 days (n=12). Plasma samples were analyzed with LC-MS/MS. Pharmacokinetic parameters of L-NBP were calculated using non-compartmental analysis with WinNonlin software. Statistical analysis was performed using SPSS software. All adverse events...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-3-n-butylphthalide/tablets/safety; L-3-n-butylphthalide/tablets/tolerability; L-3-n-butylphthalide/tablets/pharmacokinetics; Natural antioxidant.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300525

Evaluation of physicochemical properties and in-vitro release profile of glipizide-matrix patch
Provedor de dados: 60
Autores: Ghosal,Kajal; Rajabalaya,Rajan; Maiti,Anindya Kishore; Chowdhury,Bikramaditya; Nanda,Arunabha.
OBJECTIVES: The aim of the present investigation was to form matrix patches with ethyl cellulose (EC) as the base polymer, polyvinyl pyrrolidone (PVP) as the copolymer, plasticizer with dibutyl phthalate (DBP) or acetyl tributyl citrate (ATBC) and the drug glipizide (gz) by the solvent casting method. Physicochemical properties of the patches and in vitro drug release were determined in a modified Keshary-chien diffusion cell to optimize the patch formulations with the help of experimental data and figures for further studies. TECHNIQUES: EC and PVP of different proportions with different weight percentages of either DBP or ATBC and a fixed amount of glipizide were taken for matrix patch formations. The dried patches were used for measuring their drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glipizide/physicochemical properties; Glipizide/in-vitro release; Ethyl cellulose; Polyvinyl pyrrolidone; Plasticizer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200007

Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test
Provedor de dados: 60
Autores: Kuhn,Andrielle Wouters; Tedesco,Marília; Boligon,Aline Augusti; Athayde,Margareth Linde; Laughinghouse IV,Haywood Dail; Tedesco,Solange Bosio.
Punica granatum L., locally known as romanzeira, is native to Asia but found throughout Brazil. P. granatum is used for treating inflammatory, infectious and respiratory diseases. The aim of this study was to evaluate the chromatography and genotoxicity of an aqueous extract of P. granatum (pomegranate) fruit peel using the Allium cepa L. test. The experiment set-up entailed 7 treatments: T1-distilled water, T2-tea 5 g.L-1, T3-tea 10 g.L-1, T4-glyphosate at 9.6%, T5-glyphosate with subsequent recovery in distilled water, T6-glyphosate with subsequent recovery in tea 5 g.L-1 and T7-glyphosate with subsequent recovery in tea 10 g.L-1. The rootlets were collected and fixed in ethanol:acetic acid (3:1) for 24 hours, then stored in 70% ethanol under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Punica granatum L./phytochemistry; Punica granatum L./genotoxicity Punica granatum L./chromatography analysis; Medicinal plants; Pomegranate.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100241

Salivary calcium and phosphate stability in different time and temperature storage
Provedor de dados: 60
Autores: Daniel,Filipe Ivan; Lima,Luiza; Santos,Claudia Regina dos.
ABSTRACT The non-invasive collection and inexpensive nature of saliva has made it an attractive sample for use for diagnosis and research on several diseases. Storage circumstances may affect salivary component concentrations. The objective was to analyze calcium and phosphate stability in saliva samples stored at different conditions. Saliva of healthy people was stored and analyzed by spectrophotometry under different time and temperature conditions in order to evaluate calcium and phosphate stability. Calcium concentration was measured by Arsenazo III reaction at 600nm and phosphate by an acid-molybdate method at 650nm. Using Lin's concordance correlation coefficient (k), we observed very good agreement (k&gt;0.8) for all samples frozen at -20 oC up to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saliva/study/analysis; Saliva/storage/analysis; Saliva/calcium/stability/spectrophotometry; Saliva/phosphorus/stability/spectrophotometry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400679

Proteinase activity in latex of three plants of the family Euphorbiaceae
Provedor de dados: 60
Autores: Sobottka,Andréa Michel; Tonial,Fabiana; Sytwala,Sonja; Melzig,Matthias.
In the family of Euphorbiaceae,the genera Euphorbia and Sapium are known to contain essentially latex-bearing species. In the present study, the latex of Euphorbia selloi(Klotzsch &amp; Garcke) Boiss., Euphorbia papillosa A.St.-Hil., and Sapium glandulosum (L.) Morong, plants native from Brazil, were examined concerning proteolytic activity. All studied species have proteins with significant proteolytic activity and E. papillosa has the greatest specific activity. Aiming to verify the type of protease present, an assay with different inhibitors was performed. In the three tested plants, the proteolytic activity was significantly inhibited by a serine protease inhibitor 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride (AEBSF). Using techniques of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbiaceae/species/phytochemistry; Euphorbia papillosa/phytochemistry; Euphorbia selloi/phytochemistry; Sapium glandulosum/phytochemistry; Euphorbia papillosa/proteinase activity; Euphorbia selloi/proteinase activity; Sapium glandulosum/proteinase activity; Endopeptidase; Gel electrophoresis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300559

Transdermal iontophoretic delivery of timolol maleate
Provedor de dados: 60
Autores: Patni,Mayur; Puranik,Prashant; Sonawane,Aravind; Panzade,Prabhakar.
Transdermal iontophoresis would be a promising method for the systemic delivery of water soluble and ionic drugs of relatively high molecular size, including peptides. The objective of the present study was to investigate the effect of biological variable such as guinea pig and human cadaver skin and other variables like drug concentration, current density on the transdermal iontophoretic transport of timolol maleate. The permeation profile of drug using solution and gel formulation was studied and compared. For better bioavailability, better patient compliance, and enhanced delivery, an iontophoretic drug delivery system of a timolol maleate matrix gel was formulated using Carbopol 974P. The study was conducted using silver-silver chloride electrodes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Timolol maleate/transdermal iontophoretic transport; Transdermal iontophoresis/drug release; Drugs/release; Timolol maleate/skin permeation; Carbopol gel/transdermal iontophoretic delivery.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400026

Análise morfoanatômica comparativa de duas espécies de carqueja: Baccharis microcephala DC. e B. trimera (Less.) DC., Asteraceae
Provedor de dados: 60
Autores: Budel,Jane Manfron; Duarte,Márcia do Rocio.
A identificação de espécies de Baccharis que são constituídas de cladódios, ou seja, caules alados, apresenta dificuldades até mesmo para especialistas em taxonomia vegetal. Baccharis microcephala e B. trimera pertencem ao grupo Trimera e são conhecidas vulgarmente como carquejas. Na medicina popular, é comum o uso de espécies aladas para a aplicação terapêutica como diurético, hepatoprotetor e digestivo. Baccharis trimera consta na F. Bras. IV e possui atividades antiinflamatória, analgésica, bacteriostática, bactericida e antidiabética. As espécies B. trimera e B. microcephala são freqüentemente confundidas e utilizadas pela população para as mesmas finalidades terapêuticas. Objetivou-se estudar a morfologia externa e a anatomia dos cladódios dessas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asteraceae; Baccharis microcephala; Baccharis trimera; Controle de qualidade; Morfoanatomia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100009

Alkaloid-rich fraction of Himatanthus lancifolius contains anti-tumor agents against leukemic cells
Provedor de dados: 60
Autores: Lima,Melissa Pires de; Hilst,Luciana Farhat; Mattana,Fernanda Vanessa Rechinbach; Santos,Cid Aimbiré de Moraes; Weffort-Santos,Almeriane Maria.
The effects of the alkaloid-rich fraction of Himatanthus lancifolius (Müll. Arg) Woodson on normal marrow cells and leukemic cell lines were investigated. After 48 h exposure, the proliferation assay showed significant cell growth inhibition for Daudi (0.1-10 µg/mL), K-562 (1-10 µg/mL), and REH cells (10-100 µg/mL), yet was inert for normal marrow cells. A similar inhibition profile was observed in clonogenic assays. This alkaloid-rich fraction, in which uleine is the main compound, showed no signs of toxicity to any cells up to 10 µg/mL. Cell feature analyses after induction of differentiation showed maintenance of the initial phenotype. Flow cytometric expression of Annexin-V and 7-AAD in K-562 and Daudi cells has indicated that the cells were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apocynaceae; Cell proliferation; Himatanthus lancifolius; Indole alkaloids; Leukemia; Plant extract.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200014

Preformulation of a liquid dosage formulation of captopril for pediatric use: drug-excipient compatibility and stability studies
Provedor de dados: 60
Autores: Goes,Janaina da Silva; Freire,Fátima Duarte; Moura,Túlio Flávio Accioly de Lima e; Aragão,Cícero Flávio Soares; Raffin,Fernanda Nervo.
Currently, medications used in children are typically modified from pharmaceutical dosage forms designed for adults. Captopril is widely adapted to liquid formulations for use in hospitals. Its stability in the aqueous medium is reduced since it undergoes oxidation producing captopril disulfide (its main metabolite). The aim of this formulation study was to suggest favorable conditions for the development of a stable captopril formulation. The compatibility between the drug and excipients was evaluated by differential scanning calorimetry analysis (DSC). For studies in solution, different formulations were prepared according to a factorial design varying EDTA concentration, water purity and pH. The resultant formulations were stored at 60°C and analyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pediatric Formulation; Captopril/ pharmacokinetics; Thermal Analysis; Degradation Kinetics; Drug Stability; Drug Compounding/ utilization; Drug Utilization; Excipients/ pharmacokinetics; Dosage Forms; Drug Dosage Calculations; Kinetics; Enzyme Stability/drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100603

New flavonoid with antidiabetic and antioxidant potential from Tetrastigma angustifolia (Roxb.) Deb leaves
Provedor de dados: 60
Autores: Junejo,Julfikar Ali; Zaman,Kamaruz; Ali,Mohammed; Rudrapal,Mithun.
Ethnomedicinal survey documents the traditional practices of Tetrastigma angustifolia leaves in the management of diabetes in the North-eastern region of India. The present study was aimed at isolation of possible antidiabetic principle(s) from T. angustifolia leaves and evaluation of antidiabetic efficacy of isolated compound(s) in experimental animal model. The methanolic extract of T. angustifolia leaves was obtained by Soxhlet extraction method and subjected to silica gel column chromatography (100-200 mesh). Fraction 18-176 chloroform:methanol (70:30) yielded a pale yellow colored compound. The structure of pure compound was elucidated with the help of UV, IR, NMR and Mass spectrometric/techniques. The antioxidant activity of the isolated compound was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T. angustifolia; Leaves; Flavonoid; Antidiabetic; Antioxidant; Lead molecule.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100633

Measurement uncertainty of dissolution test of acetaminophen immediate release tablets using Monte Carlo simulations
Provedor de dados: 60
Autores: Romero,Daniel Cancelli; Lourenço,Felipe Rebello.
ABSTRACT Analytical results are widely used to assess batch-by-batch conformity, pharmaceutical equivalence, as well as in the development of drug products. Despite this, few papers describing the measurement uncertainty estimation associated with these results were found in the literature. Here, we described a simple procedure used for estimating measurement uncertainty associated with the dissolution test of acetaminophen tablets. A fractionate factorial design was used to define a mathematical model that explains the amount of acetaminophen dissolved (%) as a function of time of dissolution (from 20 to 40 minutes), volume of dissolution media (from 800 to 1000 mL), pH of dissolution media (from 2.0 to 6.8), and rotation speed (from 40 to 60 rpm). Using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetaminophen tablets/dissolution test/measurement uncertainty; Monte Carlo simulation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300613

Assistência ao diabetes no Sistema Único de Saúde: análise do modelo atual
Provedor de dados: 60
Autores: Guidoni,Camilo Molino; Olivera,Carolina Maria Xaubet; Freitas,Osvaldo de; Pereira,Leonardo Regis Leira.
O sistema de saúde nacional, impulsionado por contextos econômicos restritivos, passou por uma reestruturação organizacional na década de 1990, voltada à atenção primária. Apesar das reformas, a incidência de diabetes mellitus ainda atinge elevadas proporções. Devido à alta morbi-mortalidade, a prevenção dessa morbidade tem-se tornado foco atual, fazendo-se necessário o desenvolvimento de programas eficazes e viáveis ao Sistema Único de Saúde, tais como ênfase na prevenção primária, controle da incidência e complicações da doença. Para que isso ocorra, o modelo da atenção atual prestada ao paciente deve ser modificado, priorizando a atenção primária, com a substituição do modelo médico-hegemônico por uma equipe interdisciplinar, onde o farmacêutico, como...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sistema Único de Saúde; Farmacoterapia; Diabetes mellitus; Doenças crônicas; Atenção Primária à Saúde; Medicamentos/política.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100005

Stability of Tilo® tablets formulation obtained from dry extract of Justice pectoralis Jacq.
Provedor de dados: 60
Autores: Rodríguez-Chanfrau,Jorge E.; Núñez-Figueredo,Yanier; Fuste-Moreno,Viviana.
Justicia pectoralis Jacq., Acanthaceae, is a herb known popularly in Cuba as Tilo and used traditionally as sedative. The development in a solid pharmaceutical (Tablets 100 mg) using dry extract of Justicia pectolaris aqueous extract is of interest for the development of phytomedicines, which uses this active raw material. The aim of the present study was to carry out chemical and biological stability studies to the formulation. A method of coumarin determination by High Performance Liquid Chromatography (HPLC) was used and validated. The stability studies during different periods of time (24 months) showed a stability of the product stored at 32 ± 2 °C, and protected of the light.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Justicia pectoralis/pharmacognosy; Medicinal plants; Phytomedicines; Coumarins/determination; High Performance Liquid Chromatography/phytomedicines analysis; Phytomedicines/stability studies; Tilo®/tablets/stability studies.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100193

Pharmaceutical Care for hypertensive patients provided within the Family Health Strategy in Goiânia, Goiás, Brazil
Provedor de dados: 60
Autores: Martins,Bárbara Posse Reis; Aquino,Aline Teixeira de; Provin,Mércia Pandolfo; Lima,Dione Marçal; Dewulf,Nathalie de Lourdes Souza; Amaral,Rita Goreti.
The aim of Pharmaceutical Care programs is to improve patients' quality of life, and such programs are particularly effective in the case of chronic diseases such as hypertension. The objective of this longitudinal study was to analyze a Pharmaceutical Care model for hypertensive patients receiving care within the Family Health Strategy (FHS). All patients were being seen by an FHS team affiliated to a primary healthcare unit in Goiânia, Goiás, Brazil. Fourteen patients participated in the study, with each patient receiving six home visits during the Pharmaceutical Care. Overall, 142 drug-related problems were reported, the most common concerning the ineffectiveness of treatment (33.8%). A total of 135 pharmaceutical interventions were performed, 92.6% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care; Hypertension; Primary health care.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300023

Best herbs for managing diabetes: a review of clinical studies
Provedor de dados: 60
Autores: Ghorbani,Ahmad.
Diabetes mellitus is a public health problem which leads to serious complications over time. Experimentally, many herbs have been recommended for treating diabetes. In most cases, however, the recommendations are based on animal studies and limited pieces of evidence exist about their clinical usefulness. This review focused on the herbs, the hypoglycemic actions of which have been supported by three or more clinical studies. The search was done in Google Scholar, Medline and Science Direct databases using the key terms diabetes, plants, herbs, glucose and patients. According to the clinical studies, Aegle marmelos, Allium cepa, Gymnema sylvestre, Momordica charantia, Ocimum sanctum, Nigella sativa, Ocimum sanctum, Panax quinquefolius, Salacia reticulate,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants/diabetes treatment; Diabetes/treatment; Diabetes/clinical trials.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300003

Effects of caffeine, tea polyphenol and daidzein on the pharmacokinetics of lansoprazole and its metabolites in rats
Provedor de dados: 60
Autores: Wu,Tao; Wu,Ji; Gao,Ping; Xiang,Daochun; Liu,Dong; Song,Hongping.
abstract The aim of this study was to evaluate the effects of caffeine, tea polyphenol and daidzein on the pharmacokinetics of lansoprazole and its metabolites. Rats were intragastrically administered caffeine (30 mg·kg-1, once per day), tea polyphenol (400 mg·kg-1, once per day) or daidzein (13.5 mg·kg-1, once per day) for 14 days, followed by an intragastric administration of lansoprazole (8 mg·kg-1) on the 15th day. The plasma concentrations of lansoprazole and its two primary metabolites, 5-hydroxylansoprazole and lansoprazole sulfone, were determined by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Tea polyphenol significantly elevated the Area Under the Curve (AUC) of lansoprazole from 680.29 ± 285.99 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lansoprazole/pharmacokinetics; Caffeine/effects/lansoprazole pharmacokinetics; Tea polyphenol/effects/lansoprazole pharmacokinetics; Daidzein/effects/lansoprazole pharmacokinetics; 5-hydroxylansoprazole; Lansoprazole sulfone; Hepatic microsomal enzyme/inhibition.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400893

Influence of ceramide 2 on in vitro skin permeation and retention of 5-ALA and its ester derivatives, for Photodynamic Therapy
Provedor de dados: 60
Autores: Pierre,Maria Bernadete Riemma; Lopez,Renata Fonseca Vianna; Bentley,Maria Vitória Lopes Badra.
Photodynamic therapy (PDT) based on topical 5-aminolevulinic acid (5-ALA), an endogenous precursor of protoporphyrin, is an interesting approach for the treatment of skin cancer. However, 5-ALA is a hydrophilic molecule and such a characteristic limits its appropriate cutaneous penetration and retention. In this way, more lipophilic molecules, such as esterified 5-ALA derivatives, have been under investigation in order to improve the skin penetration of this molecule. Drug formulation can also alter 5-ALA skin penetration. Therefore, the aim of this work was to study the influence of ceramide 2 - the main lipid of the SC- on the cutaneous delivery of 5-ALA and its ester derivatives in vitro, using Franz diffusion cell. The skin permeation of all studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5-aminolevulinic acid; Ceramide 2; In vitro/Skin permeation; In vitro/Skin retention; Photodynamic therapy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100013

Purchase of prescription and OTC medicines in Slovakia: factors influencing patients' expectations and satisfaction
Provedor de dados: 60
Autores: Haramiova,Zuzana; Kobliskova,Zuzana; Soltysova,Jurina.
ABSTRACT Slovakia is a country where the purchase of OTC (over the counter) medicines outside the pharmacy is not allowed by the government. This study aimed at evaluating patients' satisfaction and acceptance of community pharmacists. Customer's behaviour and expectations influencing the purchase of prescription and OTC medicines were analyzed. A structured questionnaire having 15 multiple-choice questions was used to analyze the descriptive parameters. Data collection lasted from January to February 2014. The sample size consisted of 357 high-school educated individuals under 40 years of age. The survey showed that the prescription and OTC medicines were bought equally. The participants reported a 96.0%, 96.3% and 90.2% satisfaction rate with willingness...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical Care/study/Slovakia; Community Pharmacy/Slovakia; Community Pharmacists/study/Slovakia; Patients/use of medicines; Patient satisfaction; Patient expectation; Medicines/prescription medicines.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100617

Microbial biotransformation of beclomethasone dipropionate by Aspergillus niger
Provedor de dados: 60
Autores: Ahmad,Saeed; Khaliq,Farhan Hameed; Madni,Asadullah; Shahid,Muhammad Nabeel; Pervaiz,Irfan.
In the present research, the steroidal anti-asthmatic drug beclomethasone dipropionate was subjected to microbial biotransformation by Aspergillus niger. Beclomethasone dipropionate was transformed into various metabolites first time from microbial transformation. New drug metabolites produced can act as new potential drug molecules and can replace the old drugs in terms of safety, efficacy, and least resistance. They were purified by preparative thin layer chromatography technique, and their structures were elucidated using modern spectroscopic techniques, such as 13C NMR, 1H NMR, HMQC, HMQC, COSY, and NOESY, and mass spectrometry, such as EI-MS. Four metabolites were purified: (i) beclomethasone 17-monopropionate, (ii) beclomethasone 21-monopropionate,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-asthmatics; Aspergillus niger/use in biotransformation; Beclomethasone dipropionate/microbial biotransformation; Steroids/microbial transformation; Biotransformation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400903

Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease
Provedor de dados: 60
Autores: Siddique,Yasir Hasan; Jyoti,Smita; Naz,Falaq.
In the present study we have studied the effect of 25, 50, 75 and 100 µM of luteolin on the transgenic Drosophila expressing human alpha synuclein. The doses of luteolin were established in diet and the PD flies were allowed to feed on it for 24 days. After 24 days of exposure the flies were assayed for climbing assay, oxidative stress markers, caspase-3 &amp; 9 activity and dopamine content. The immunohistochemistry was also performed on the brain sections for the activity of tyrosine hydroxylase. The exposure of luteolin showed a dose dependent delay in the loss of climbing ability and activity, reduction in oxidative stress markers, caspase-3&amp;9 activities and results in an increase in the dopamine content. The results obtained for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Luteolin; Parkinson’s disease; Drosophila; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300627

Direct effect of  p,p'- DDT on mice liver
Provedor de dados: 60
Autores: Arroyo-Salgado,Bárbara; Olivero-Verbel,Jesús; Guerrero-Castilla,Angélica.
ABSTRACT Contact with the pesticide dichlorodiphenyltrichloroethane (p,p′-DDT) can be the cause of various harmful effects in humans, wildlife, and the environment. This pesticide is known to be persistent, lipophilic, resistant to degradation, and bioaccumulive in the environment and to be slowly released into bloodstream. Growing evidence shows that exposure to DDT is linked to type 2 diabetes mellitus. Individuals exposed to elevated levels of DDT and its metabolite have an increased prevalence of diabetes and insulin resistance. To evaluate these possible relationships, experiments were performed on eight-week-old female mice, divided into three groups (n = 10 per group): Group 1 received a vehicle-control intraperitoneal (i.p.) injection of sesame...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticides; Persistent organic pollutant; Dichlorodiphenyltrichloroethane/exposure; Type 2 diabetes mellitus/exposure to dichlorodiphenyltrichloroethane/experimental study; Insulin/resistance/exposure to dichlorodiphenyltrichloroethane; Gene expression.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200287

Stability indicating RP-LC-PDA method for the quantitative analysis of saxagliptin in pharmaceutical dosage form
Provedor de dados: 60
Autores: Scheeren,Laís Engroff; Marcolino,Ana Isa Pedroso; Adams,Andréa Inês Horn; Rolim,Clarice Madalena Bueno.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saxagliptin/tablets/quality control; High Performance Liquid Chromatography/quantitative analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200461

Isolation of flavonoids from Anemopaegma arvense (Vell) Stellf. ex de Souza and their antifungal activity against Trichophyton rubrum
Provedor de dados: 60
Autores: Costanzo,Camila Di Giovane; Fernandes,Vanessa Colnaghi; Zingaretti,Sônia; Beleboni,Rene Oliveira; Pereira,Ana Maria Soares; Marins,Mozart; Taleb-Contini,Sílvia Helena; Pereira,Paulo Sérgio; Fachin,Ana Lúcia.
Anemopaegma arvense (Vell) Stellf. ex de Souza belongs to the family Bignoniaceae, and is popularly known as catuaba. To evaluate the cytotoxic and antimicrobial activity of A. arvense, fraction F3 and flavonoids 1 (quercetin 3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside) (rutin) and flavonoid 2 (quercetin 3-O-α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside) were isolated from the leaves of this plant. Fraction F3 and flavonoids 1 and 2 exhibited no antibacterial activity. Furthermore, no cytotoxic activity of fraction 3 or flavonoids 1 and 2 was observed against the tumor cells tested. However, analysis of the antifungal activity of flavonoids 1 and 2 revealed minimum inhibitory concentrations of 0.5 and 0.25 mg/mL, respectively, against the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bignoniaceae; Anemopaegma arvense/phytochemistry; Catuaba/phytochemistry; Flavonoids/antifungal activity; Plant extract/evaluation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300017

Experimental design approach for development of novel microemulsion system and immediate release self microemulsifying tablet of nebivolol HCl
Provedor de dados: 60
Autores: Trivedi,Hemangi Ramesh; Siriah,Tanvi Mukund; Puranik,Prashant Keshao.
The objective of this study was to determine specific combination of pharmaceutical excipients that lead to formulation of efficient nebivolol hydrochloride SMEDDS and its subsequent formulation into IR-SET (Immediate release- Self emulsifying tablet) which will enhance its solubility and dissolution. Solubility and Pseudo-ternary phase studies were carried out to identify the excipients showing highest solubility and to identify the zone of microemulsion with selected ingredients. Liquid-SMEDDS (L-SMEDDS) were optimized for Concentration of oil(X1) and Smix(X2) and formulated using a combination of Kollisolv GTA as oil, Tween 80 as surfactant and propylene glycol as co-surfactant which gave smaller droplet size(Y1) 55.98nm , Emulsification time (Y2)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nebivolol hydrochloride Liquid &amp;amp; Solid SMEDDS Full factorial design Neusilin US2 IR-SET.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100547

Design of Experiments (DoE) applied to Pharmaceutical and Analytical Quality by Design (QbD)
Provedor de dados: 60
Autores: Fukuda,Isa Martins; Pinto,Camila Francini Fidelis; Moreira­,Camila dos Santos; Saviano,Alessandro Morais; Lourenço,Felipe Rebello.
According to Quality by Design (QbD) concept, quality should be built into product/method during pharmaceutical/analytical development. Usually, there are many input factors that may affect quality of product and methods. Recently, Design of Experiments (DoE) have been widely used to understand the effects of multidimensional and interactions of input factors on the output responses of pharmaceutical products and analytical methods. This paper provides theoretical and practical considerations for implementation of Design of Experiments (DoE) in pharmaceutical and/or analytical Quality by Design (QbD). This review illustrates the principles and applications of the most common screening designs, such as two-level full factorial, fractionate factorial, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Design of Experiments (DoE); Quality by Design (QbD); Factorial Designs; Central Composite Designs (CCD); Box-Behnken Designs; Response Surface Methodology (RSM).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700405

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry
Provedor de dados: 60
Autores: Tótoli,Eliane Gandolpho; Salgado,Hérida Regina Nunes.
abstract Daptomycin is the first approved drug from a new class of antimicrobials, the cyclic lipopeptides, and is a very important antimicrobial agent in current clinical practice. Currently, there are no "green" analytical methods described in the literature to analyze the typical pharmaceutical dosage form of daptomycin. Thus, the aim of this work was to validate an environment-friendly spectrophotometric method in the UV region, for the analysis of daptomycin as a lyophilized powder. Water was used as diluent and the analyses were carried out on a spectrophotometer at 221 nm. The method met all validation requirements of the ICH guidelines, over a concentration range of 6-21 µg mL-1. A Student's t-test demonstrated that the proposed method was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobials/quality control; Daptomycin/determination; Spectrophotometry/UV region/quantitative analysis; Spectrophotometry/green analytical method.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400811

Spectrofluorimetric determination of amlodipine in human plasma without derivatization
Provedor de dados: 60
Autores: Kadioglu,Yucel; Ozturk,Murat.
A rapid and sensitive spectrofluorimetric method was developed for the determination of amlodipine (AD), a calcium channel blocker, in the plasma. The type of solvent, the wavelength range, and the range of AD concentration were selected to optimize the experimental conditions. The calibration curves were linear (r² &gt;0.997) in the concentration range of 0.1-12.5 ppm of AD. The limit of quantitation and limit of detection values for the method for plasma samples were 0.1 ppm and 0.07 ppm, respectively. The precision calculated as the relative standard deviation was less than 3.5%, and the accuracy (relative error) was better than 5.5% (n=6). The method developed in this study can be directly and easily applied for the determination of AD in the plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amlodipine/determination; Spectrofluorimetric method/validation; Human plasma.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400016

Validation of high-performance liquid chromatographic method for analysis of fluconazole in microemulsions and liquid crystals
Provedor de dados: 60
Autores: Silva,Hilris Rocha e; Santos,Fernanda Kolenyak dos; Luz,Gabriela Marielli da; Chorilli,Marlus; Gremião,Maria Palmira Daflon.
In recent decades, there has been a significant increase in the incidence of fungal diseases. Certain fungal diseases cause cutaneous lesions and in the usual treatment, generally administred orally, the drug reaches the site of action with difficulty and its concentration is too low. An approach much explored in recent years is the development of nanotechnology-based drug delivery systems, and microemulsions (ME) and liquid crystals (LC) are promising. ME and LC were developed with oleic acid or copaiba oil as the oil phase, propoxyl (5OP) ethoxyl (20 OE) cetyl alcohol as surfactant and water. An analytical method to assess the incorporation of fluconazole (FLU) in the systems under study was validated according to guidelines of the International...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanotehcnology; Microemulsions; Liquid crystals; High-performance liquid chromatography/qualitative analysis; Fluconazole.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200381

Goitrogenic/antithyroidal potential of moringa leaves (Moringa oleifera) and spinach (Spinacia oleracea) of Indian origin on thyroid status in male albino rats
Provedor de dados: 60
Autores: Mondal,Chiranjit; Chandra,Amar Kumar.
Moringa leaves and spinach used as vegetables containing cyanogens and polyphenols having suspected antithyroidal activity; however, detailed studies in this aspect found unavailable. Goitrogenic/antithyroid potentiality of those plant foods of Indian origin was evaluated. To explore the goitrogenic/antithyroid effect of those plant foods cyanogenic glucosides, glucosinolates, thiocyanate and polyphenol content were measured. These plant foods were fed with diet regularly to adult male albino rats for 30 and 60 days respectively, followed by evaluation of morphological and functional status of thyroid,as thyroid gland weight, the activity status of thyroid hormone synthesizing enzymes viz. TPO, Na+-K+-ATPase, deiodinase I, thyroid hormone profiles, thyroid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goitrogen; Hypothyroidism; Iodine; Moringa; Spinach.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100559

Identification of neprilysin as a potential target of arteannuin using computational drug repositioning
Provedor de dados: 60
Autores: Ye,Xuan-Yi; Ling,Qing-Zhi; Chen,Shao-Jun.
ABSTRACT The discovery of arteannuin (qinghaosu) in the 20th Century was a major advance for medicine. Besides functioning as a malaria therapy, arteannuin is a pharmacological agent in a range of other diseases, but its mechanism of action remains obscure. In this study, the reverse docking server PharmMapper was used to identify potential targets of arteannuin. The results were checked using the chemical-protein interactome servers DRAR-CPI and DDI-CPI, and verified by AutoDock Vina. The results showed that neprilysin (also known as CD10), a common acute lymphoblastic leukaemia antigen, was the top disease-related target of arteannuin. The chemical-protein interactome and docking results agreed with those of PharmMapper, further implicating neprilysin as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arteannuin/chemical-protein interactome; Computational drug repositioning; Neprilysin/identification; Reverse docking.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200614

Antioxidant, analgesic and anti-inflammatory activities of Leucas cephalotes (Roxb.ex Roth) Spreng
Provedor de dados: 60
Autores: Baburao,Bhukya; Reddy,Anreddy Rama Narsimha; Kiran,Gangarapu; Reddy,Yellu Narsimha; Mohan,Gottumukkala Krishna.
The whole plant of the methanolic extract from Leucas cephalotes was screened for invitro antioxidant (using the DPPH method), invivo analgesic (using hot plate test in mice) and anti-inflammatory (using rat paw edema test) activities. The methanolic extract of Leucas cephalotes (MELC) scavenged the DPPH radicals in a dose-dependent manner. The IC50 value to scavenge DPPH radicals was found to be 421.3µg/ml. A significant (p&lt;0.0005) analgesic activity was observed at 60 min with 200 mg/kg, and 400 mg/kg exhibited maximum activity. The maximum anti-inflammatory response was produced at 3 hr and 2 hr with doses of 200 and 400 mg/kg, respectively. These results suggest that the methanolic extract from Leucas cephalotes exerts significant analgesic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leucas cephalotes/analgesic activity; Leucas cephalotes/antioxidant activity; Leucas cephalotes/anti-inflammatory activity; Diphenyl Picryl Hydrazyl/assay.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300016

NLRP3 inflammasome in metabolic syndrome
Provedor de dados: 60
Autores: Wang,Fang; Mo,Zhaohui.
Metabolic syndrome (MS) is a serious health problem worldwide; it is characterized by a group of metabolic disorders, including central obesity, insulin resistance/type 2 diabetes, hyperlipidemia with accelerated atherosclerosis, hypertension, non-alcoholic fatty liver disease, and elevated uric acid with increased risk of gout. The incidence of MS has increased considerably in recent decades and has attracted considerable attention. A number of clinical and translational laboratory studies have implicated the activation of nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome in the development of MS, therefore establishing a strong link between chronic inflammation and metabolic diseases. This paper aims to review new...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; NLRP3 inflammasome; Chronic inflammation; IL-1β.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100639

Social inequalities in lawsuits for drugs
Provedor de dados: 60
Autores: Provin,Mércia Pandolfo; Leite,Silvana Nair; Amaral,Rita Goreti.
The aim of this study was to characterize the lawsuits requesting drugs considering the economic profile of their petitioners. All lawsuits (1378) accepted against Goiânia, GO from 2003 to 2007 were analyzed. Petitioners' demographic characteristics, reported diseases, requested drugs, origin of healthcare service, and lawsuit agent were described. Complainants' addresses were georeferenced and distributed into 4 regional groups classified in accordance with the population's average income. Dwellers of wealthier regions filed court actions requesting drugs more frequently, with an average rate of 1.7 lawsuits/1000 inhabitants versus 0.55/1000 in the poorer region. Lawsuit costs were 4-fold higher in wealthier regions compared with the poorest region....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Health rights; Drugs/access; Health inequalities; Health care.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300008

Handbook of herbs and spices
Provedor de dados: 60
Autores: Fischer,Dominique Corinne Hermine.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400030

Production, quality control, biodistribution assessment and preliminary dose evaluation of [177Lu]-tetra phenyl porphyrin complex as a possible therapeutic agent
Provedor de dados: 60
Autores: Zolghadri,Samaneh; Yousefnia,Hassan; Jalilian,Amir Reza; Fazaeli,Yousef.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals/internal dosimetry Lutetium-177 &lt; Sup&gt; 177&lt; /sup&gt; Lu-tetraphenyl porphyrin/biodistribution Porphyrins/biodistribution Medical internal radiation dose MIRD.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200339

Pharmacological activity of the hydroalcoholic extract from Hovenia dulcis thunberg fruit and the flavonoid dihydromyricetin during hypercholesterolemia induced in rats
Provedor de dados: 60
Autores: Pinto,Juliana Tensol; Oliveira,Tânia Toledo de; Alvarenga,Luana Farah; Barbosa,Adriano Simões; Pizziolo,Virgínia Ramos; Costa,Marcelo Rocha da.
Cerebrovascular accidents and coronary artery disease are the leading causes of cardiovascular mortalities in Brazil and high levels of LDL cholesterol are one of the main risk factors. In this context, several plant extracts and natural substances have shown promise as cholesterol-lowering. The objective of this study was to evaluate the potential of the hydroalcoholic extract of the fruit of H. dulcisand of dihydromyricetin in cholesterol reduction in hypercholesterolemic rats. Forty-two Wistar male rats were distributed into seven groups of six animals that received diets supplemented with 1% cholesterol and 0.3% cholic acid, with the exception of the control group, which received conventional diets. Animals were treated with oral suspensions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hovenia dulcis/pharmacognosy; Hovenia dulcis/hydroalcoholic extract/ cholesterol reduction; Hovenia dulcis/flavonoid dihydromyricetin/cholesterol reduction; Dihydromyricetin/cholesterol reduction; Hypercholesterolemia/experimental study; Medicinal plants.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400727

One year of BJPS!
Provedor de dados: 60
Autores: Ferreira,Elizabeth Igne.
Tipo: Info:eu-repo/semantics/other
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400001

Antitumor activity of leaves of Himatanthus drasticus (Mart.) Plumel-Apocynaceae (janaguba) in the treatment of Sarcoma 180 tumor
Provedor de dados: 60
Autores: Sousa,Eliane Leite de; Grangeiro,Ana Ruth Sampaio; Bastos,Isla Vanessa Gomes Alves; Rodrigues,Guilherme Carvalho Ribeiro; Silva,Maria Joselice e; Anjos,Falba Bernadete Ramos dos; Souza,Ivone Antonia de; Sousa,Cicero Ernandes Leite de.
Himatanthus drasticus, also known as janaguba, is used popularly in Brazil's Northeastern region in the treatment of cancer. However, no scientific reports are available. The present study is the first investigation on the antitumor activity of crude methanolic extract from Himatanthus drasticus leaves against Sarcoma 180 tumor and on its side effects including acute oral toxicity. The OECD 423 methodology was used to study acute oral toxicity, and the STOCK methodology to assess antitumor activity. The crude extract showed low toxicity at the tested doses (50, 300 and 2000 mg/kg) administered orally. The histopathological analyses demonstrated alterations in liver lung, spleen and kidney. It also showed activity against Sarcoma 180 tumor in male Swiss...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Himatanthus drasticus/antitumor activity; Himatanthus drasticus/acute oral toxicity; Janaguba/antitumor activity; Sarcoma 180/experimental study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200005

Preparation and in vitro &amp;amp; in vivo evaluation of cephalexin matrix tablets
Provedor de dados: 60
Autores: Reddy,Boi Basanta Kumar; Nagoji,Kambhayathughar Eswara Venkata; Sahoo,Satyanarayan.
The purpose of the study is to develop cephalexin controlled-release matrix tablets by using lower proportions of release retardant polymer and to establish their in vitro &amp; in vivo correlation. Tablets were compressed by incorporating polymers in a matrix form along with drug which prolong the drug release. Twelve formulations were prepared by mixing ethyl cellulose (EC) and hydroxypropyl methylcellulose (HPMC) (three different viscosity grades) in various proportions. F-1 to F-4 formulations were prepared by incorporating drug, HPMC K4M and ethyl cellulose in 100 : 5 : 5, 100 : 10 : 5, 100 : 15 : 5 and 100 : 20 : 5; similarly, F-5 to F-8 were prepared with HPMC K15M; and F-9 to F-12 were prepared with HPMC K100M using a wet granulation process...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix tablets Polymer mixture Release kinetics Pharmacokinetics parameters In vitro &amp;amp; In vivo correlation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300609

Solubility and dissolution enhancement of flurbiprofen by solid dispersion using hydrophilic carriers
Provedor de dados: 60
Autores: Daravath,Bhaskar; Naveen,Chella; Vemula,Sateesh Kumar; Tadikonda,Rama Rao.
ABSTRACT The intent of the current work is to study the effect of polyethylene glycol 8000 and polyethylene glycol 10000 as hydrophilic carriers on dissolution behaviour of flurbiprofen. In the present study, solvent evaporation method was used to prepare flurbiprofen solid dispersions and evaluated for physico-chemical properties, drug-carrier compatibility studies and dissolution behaviour of drug. Solubility studies showed more solubility in higher pH values and formulations SD4 and SD8 were selected to prepare the fast dissolving tablets. FTIR and DSC study showed no interaction and drug was dispersed molecularly in hydrophilic carrier. XRD studies revealed that there was change in the crystallinity of the drug. The results of In vitro studies showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyethylene glycol/effects; Flurbiprofen/solubility; Flurbiprofen/dissolution rate; Drug-carrier compatibility/study; Dissolution efficiency.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400625

Adherence to drug treatment in hypertensive patients on the Family Health Program
Provedor de dados: 60
Autores: Ungari,Andrea Queiróz; Fabbro,Amaury Lelis Dal.
The aim of the present study was to analyze adherence to drug treatment in hypertensive patients enrolled on the Family Health Program in Ribeirão Preto, São Paulo State. This transversal study was conducted between August and December 2006, in which 109 patients were interviewed using the Morisky and Green Test to measure adherence level to pharmacotherapy. Variables that may be related to adherence level were also investigated, such as demographic characteristics, health care team and health service related factors, and pharmacotherapy related factors. The test scores were analyzed by two criteria: patients were categorized as "more adherent" if they had a score from 3 to 4 (criterion 1) or a score of 4 (criterion 2); patients with other scores were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Treatment adherence; Hypertension/drug treatment; Family Health Program.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400024

Brazilian scientific production on pharmaceutical care from 1990 to 2009
Provedor de dados: 60
Autores: Funchal-Witzel,Maria Denise Ricetto; Castro,Lia Lusitana Cardozo de; Romano-Lieber,Nicolina Silvana; Narvai,Paulo Capel.
Brazilian scientific production on pharmaceutical care was identified based on articles indexed on the Medline, Embase, Lilacs, Web of Science and International Pharmaceutical Abstracts databases. Sixty-three articles published in both national and international journals were retrieved. With regard to authors, 72.3% were from the Southeast and South Regions, and 60.8% were affiliated to public universities. In relation to the type of studies, 85.7% were descriptive, and the most frequently researched fields were community pharmacies, hospitals and primary health care units. Articles were original in 65.1% of cases, updates in 20.6%, and reviews in 7.9%. An increase in publications commenced in 2006. In 31.7% of cases, authors had adopted a bibliographical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical care; Pharmaceutical assistance; Public health; Pharmaceutical services.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200023

Relationship between oxidative stress and inflammation in peripheral and cerebral system of oxonate-induced hyperuricemic rats
Provedor de dados: 60
Autores: Jing,Wu; Zhong,Jia; Ping,Liang Jian; Yan,Liu Hong.
ABSTRACT To study what kind of role uric acid play on the relationship between oxidative Stress and inflammation in peripheral and cerebral system of oxonate-induced hyperuricemic rats. Twenty-six eight male Wistar rats were divided into two groups randomly. Potassium oxonate was used to establish hyperuricemic model for four weeks. In 2nd and 4th week, uric acid (UA) level, total superoxide dismutase (T-SOD), Gu,Zn-SOD activity and interleukin-1beta (IL-1β) concentration in serum were determined respectively. In 4th week, one hour after last PO treatment, five rats of every group were given Evans Blue to test blood-brain barrier (BBB) permeability. Other brains were obtained to analysis T-SOD, Gu,Zn-SOD activity and IL-1β concentration in cerebral system....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperuricemic Blood brain barrier Potassium oxonate Oxidative stress Inflammatory Total-SOD Gu; Zn-SOD IL-1β Pathological injury.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400620

Membrane adsorber for endotoxin removal
Provedor de dados: 60
Autores: Almeida,Karina Moita de; Almeida,Maiara Maciel; Fingola,Fernando Faria; Ferraz,Helen Conceição.
ABSTRACT The surface of flat-sheet nylon membranes was modified using bisoxirane as the spacer and polyvinyl alcohol as the coating polymer. The amino acid histidine was explored as a ligand for endotoxins, aiming at its application for endotoxin removal from aqueous solutions. Characterization of the membrane adsorber, analysis of the depyrogenation procedures and the evaluation of endotoxin removal efficiency in static mode are discussed. Ligand density of the membranes was around 7 mg/g dry membrane, allowing removal of up to 65% of the endotoxins. The performance of the membrane adsorber prepared using nylon coated with polyvinyl alcohol and containing histidine as the ligand proved superior to other membrane adsorbers reported in the literature. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endotoxins/removal; Membrane adsorber/performance; Histidine; Poly(vinyl alcohol).
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100019

Animal experimentation and scientific knowledge: a thought style?
Provedor de dados: 60
Autores: Tréz,Thales de Astrogildo e.
Animal experimentation, besides a research method extensively applied in the production of scientific knowledge, is also considered essential to science and with undeniable historical relevance in advances in human health. In this survey, a questionnaire was applied to a group of researchers involved with research based on non-animal models (n =18), and to another group involved with research based on animal models (n =18). The data analysis was grounded in Ludwik Fleck (1896 -1961) epistemological assumptions. The results suggested that there are at least two thought styles operating in consonance on the same research problem (advances in human health conditions) with significantly different conceptions not only concerning the research practices involved,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal experimentation; Ludwik Fleck; Science/public understanding; Epistemology; Research methodology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400004

Insulin analogues versus human insulin in type 1 diabetes: direct and indirect meta-analyses of efficacy and safety
Provedor de dados: 60
Autores: Sanches,Andréia Cristina Conegero; Correr,Cassyano Januário; Venson,Rafael; Gonçalves,Patrícia Rodrigues; Garcia,Mariana Martins; Piantavini,Mário Sérgio; Pontarolo,Roberto.
All patients with Diabetes Mellitus (DM) receive insulin therapy. In this study, we evaluated the efficacy, safety and tolerability of human insulin and insulin analogues. We performed a systematic review of the literature and a meta-analysis according to the Cochrane Collaboration methodology. In the absence of clinical studies comparing insulins, we performed a mixed treatment comparison to establish the differences between the active treatments. We included studies published from 1995 to 2010. HbA1c results, episodes of hypoglycemia and nocturnal hypoglycemia data were extracted and analyzed. Thirty-five randomized clinical trials were selected after examining the abstract and a full text review. These studies included 4,206 patients who received...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulins/meta-analysis; Diabetes mellitus/type 1; Insulin/treatment efficacy; Insulin/safety use.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300011

Tissue distribution and excretion of the five components of Portulaca oleracea L. extract in rat assessed by UHPLC
Provedor de dados: 60
Autores: Zhang,Wenjie; Li,Di; Leng,Aijing; Ai,Junjun; Du,Yang; Meng,Yihan; Gao,Yucong; Ying,Xixiang.
The aim of the present study was to investigate the tissue distribution and excretion of five components of Portulaca oleracea L. extract (POE) in rat following oral administration. A rapid, sensitive and specific ultra-high performance liquid chromatography (UHPLC) method with puerarin as the internal standard was used for the quantitative analysis of five components of POE, including caffeic acid (CA), p-coumaric acid (p-CA), ferulic acid (FA), quercitrin (QUER) and hesperidin (HP) in rat tissues including the liver, intestine, stomach, muscle, heart, lung, brain, kidney and spleen, urine and feces. The results show that onlyp-CA and FA were found in nearly all tissues with low cumulative ratios, and CA was higher in the intestine and stomach with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Portulaca oleracea L./pharmacokinetics; Portulaca oleracea L./extract/experimental study; Portulaca oleracea L./extract/ tissue distribution; Portulaca oleracea L./extrato/excretion; Compostos fenólicos; Ultra performance liquid chromatography/quantitative analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300643

Negative effects of bisphenol A on testicular functions in albino rats and their abolitions with Tribulus terristeris L.
Provedor de dados: 60
Autores: Munir,Bushra; Qadir,Abdul; Tahir,Mohammad.
ABSTRACT This study was conducted to find out the ameliorative properties of Tribulus terristeris L (TT) on BPA induced spermatotoxicity in male albino rats. Mature male albino rats were divided into five groups, Group A was taken as control for comparison group, whereas the other four groups namely B(vehicle control), C (toxic), D (preventive control) and Group E (amelioration group) received distilled water, olive oil, BPA, TT, and (TT + BPA) respectively. Macroscopic results revealed decreased body weight of rats, weight of testes, and the relative tissue weight index (RTWI) in BPA induced group. Hormonal (testosterone) assay results revealed the decreased values of BPA treated group. Microscopic examination of testis of BPA treated rats showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bisphenol A/negative effects; Tribulus terristeris L./properties; Spermatotoxicity; Testosterone.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300611

Phytochemical and morpho-anatomical study of the vegetative organs of Psychotria fractistipula L.B.Sm., R.M. Klein &amp;amp; Delprete (Rubiaceae)
Provedor de dados: 60
Autores: Oliveira,Camila Freitas de; Oliveira,Vinicius Bednarczuk; Bobek,Vanessa Barbosa; Rech,Katlin Suellen; Betim,Fernando Cesar Martins; Dias,Josiane de Fatima Gaspari; Zanin,Sandra Maria Warumby; Miguel,Obdulio Gomes; Miguel,Marilis Dallarmi.
Psychotria fractistipula L.B.Sm, R.M. Klein &amp; Delprete (Rubiaceae) is found in the existing Atlantic Forest in the South of Brazil, in the Paraná and Santa Catarina States. The members of Rubiaceae are characterized chemically by the presence of alkaloids, some pharmacological properties of which include antifungal, antibacterial, analgesic, antioxidant, antimutagenic, and antiviral activities. In this study, we report the results of anatomical, morphological, and histochemical analyses of the leaves and stems of the Rubiaceae family member, P. fractistipula. The anatomical analysis involved of light microscopy and scanning electron microscopy. Morpho-anatomical and histochemical characterization were made using standard methodology. The isolated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychotria; Morpho-anatomical; Phytochemistry.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100565

Development studies of captopril certified reference material
Provedor de dados: 60
Autores: Nogueira,Raquel; Rego,Eliane Cristina Pires do; Sousa,Marcus Vinicius Barreto; Wollinger,Wagner; Silva,Thaís Elias da; Moreira,Gabriela Fernandes; Barin,Juliano Smanioto; Laporta,Luciane Varini; Mesko,Márcia Foster; Bittencourt,Celso Figueiredo; Rodrigues,Janaína Marques; Cunha,Valnei Smarçaro da.
This paper describes the studies performed with the candidate Certified Reference Material (CRM) of captopril, the first CRM of an active pharmaceutical ingredient (API) in Brazil, including determination of impurities (organic, inorganic and volatiles), homogeneity testing, short- and long-term stability studies, calculation of captopril content using the mass balance approach, and estimation of the associated measurement uncertainty.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Captopril/qualitative analysis; Medicines/Certified Reference Standards; Medicines/analytical quality control; High Pressure Liquid Chromatography (HPLC)/qualitative analysis; Medicines/contamination.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200015

Ação de fatores enzimáticos na obtenção de hidrolisados protéicos de farinha de trigo com baixo teor de fenilalanina
Provedor de dados: 60
Autores: Carreira,Raquel Linhares; Silva,Viviane Dias Medeiros; Januário,José Nélio; Aguiar,Marcos José Burle de; Starling,Ana Lúcia Pimenta; Silvestre,Marialice Pinto Coelho.
Visando ao preparo de farinha de trigo com baixo teor de fenilalanina (Phe), extraiu-se, enzimaticamente as proteínas, empregando-se uma protease alcalina de Bacillus licheniformis. Em seguida, os extratos protéicos foram hidrolisados pela ação de enzimas comerciais (pancreatina e bromelina) e de extratos enzimáticos obtidos da casca de abacaxi (bruto e purificado), avaliando-se alguns parâmetros enzimáticos, tais como tipo de enzima, tipo de ação enzimática, tipo de associação enzimática e ordem de ação enzimática. O carvão ativado (CA) foi empregado como meio adsorvente e a eficiência da remoção de Phe foi avaliada por espectrofotometria derivada segunda, determinando-se o teor de Phe na farinha de trigo e em seus hidrolisados, após tratamento com CA. O...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Farinha de trigo/preparo; Hidrolisados protéicos; Enzimas; Hidrólise enzimática; Protease alcalina; Fenilalanina/remoção; Carvão ativado/meio adsorvente.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100011

Microspheres prepared with biodegradable PHBV and PLA polymers as prolonged-release system for ibuprofen: in vitro drug release and in vivo evaluation
Provedor de dados: 60
Autores: Bazzo,Giovana Carolina; Macedo,Aline Teixeira de; Crenca,Janine Paula; Silva,Virgínia Emiliana; Pereira,Eduardo Manoel; Zétola,Melissa; Pezzini,Bianca Ramos.
In this study, poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(l-lactide) (PLA) microspheres containing ibuprofen were prepared with the aim of prolonging the drug release. The oil-in-water (O/W) emulsion solvent evaporation technique was used, varying the polymer ratio. All formulations provided spherical particles with drug crystals on the surface and a porous and rough polymeric matrix when PHBV was used and smooth external surface when prepared with PLA. The in vitro dissolution profiles show that the formulation containing PHBV/PLA at the proportion of 30/70 presented the best results in terms of prolonging the ibuprofen release. The analysis of the concentration of ibuprofen in the blood of rats showed that maximum levels were achieved at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microspheres/preparation; Poly(hydroxybutyrate-co-hydroxyvalerate); Poly(l-lactide); Ibuprofen/release; Drugs/controlled release; Drugs/in vitro study; Drugs/controlled release; Drugs/in vivo evaluation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400021

Estimating the turnover number in enzyme kinetic reactions using transient and stationary state data
Provedor de dados: 60
Autores: Uludag-Demirer,Sibel; Duran,Jorge; Tanner,Robert D.
Substrate and product concentration data obtained by simulating enzyme-substrate reaction rate equations were used to test two proposed kinetic rate constant estimation techniques in this study. In the first technique, the turnover number, k3, was calculated using early transient time domain data, which are difficult to obtain experimentally. The technique used an iterative approach to calculate k3 with a pair of data and the value of k3 could be retrieved with 35% error. The second technique calculated k3 using stationary domain data and the value of k3 could be retrieved with less than 5% error. This second technique also offered internal consistency in the calculation of k3 by calculating k3 both from the intercept and the slope of the linear plot...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Kinetics/rate constant; Enzyme reactions.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400005

Arginine enzymatic deprivation and diet restriction for cancer treatment
Provedor de dados: 60
Autores: Zam,Wissam.
ABSTRACT Recent findings in amino acid metabolism and the differences between normal, healthy cells and neoplastic cells have revealed that targeting single amino acid metabolic enzymes in cancer therapy is a promising strategy for the development of novel therapeutic agents. Arginine is derived from dietary protein intake, body protein breakdown, or endogenous de novo arginine production and several studies have revealed disturbances in its synthesis and metabolism which could enhance or inhibit tumor cell growth. Consequently, there has been an increased interest in the arginine-depleting enzymes and dietary deprivation of arginine and its precursors as a potential antineoplastic therapy. This review outlines the most recent advances in targeting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arginine-depleting enzyme/antineoplastic therapy; Dietary deprivation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300402

Assessment of quality of life in type II diabetic patients using the modified diabetes quality of life (MDQoL)-17 questionnaire
Provedor de dados: 60
Autores: Prajapati,Vivek Bhanubhai; Blake,Raushan; Acharya,Leelavathi Dinesh; Seshadri,Shubha.
ABSTRACT Diabetic complications, comorbidities, and cost of treatment affect the quality of life (QoL) of an individual. The QoL assessment is considered an important measure of outcome in chronic disease management. The objective of our study was to assess the quality of life in Type II diabetes mellitus patients with and without complications using the modified diabetes quality of life (MDQoL)-17. A prospective descriptive study was conducted over 6 months, after taking ethical committee approval. As per the inclusion criteria from medicine wards of tertiary care hospital, 250 patients were selected. Demographic characteristics were documented in the data collection form and the patients were administered with the MDQoL questionnaire in different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality of Life; Diabetes complications/prevention and control; Diabetes Mellitus; Surveys and Questionnaires/utilization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400613

Basic considerations in the dermatokinetics of topical formulations
Provedor de dados: 60
Autores: Nair,Anroop; Jacob,Shery; Al-Dhubiab,Bandar; Attimarad,Mahesh; Harsha,Sree.
Assessing the bioavailability of drug molecules at the site of action provides better insight into the efficiency of a dosage form. However, determining drug concentration in the skin layers following topical application of dermatological formulations is a great challenge. The protocols followed in oral formulations could not be applied for topical dosage forms. The regulatory agencies are considering several possible approaches such as tape stripping, microdialysis etc. On the other hand, the skin bioavailability assessment of xenobiotics is equally important for topical formulations in order to evaluate the toxicity. It is always possible that drug molecules applied on the skin surface may transport thorough the skin and reaches systemic circulation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatological formulations/pharmacokinetics; Dermatological formulations/toxicity; Dermatokinetics; Skin/metabolism; Microdialysis; Tape stripping.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300004

Astrocytic expression of GFAP and serum levels of IL-1β and TNF-α in rats treated with different pain relievers
Provedor de dados: 60
Autores: Amaral,Gisele Ferreira; Dossa,Pietro Domingues; Viebig,Lígia Bocamino; Konno,Fabiana Toshie Camargo; Consoli,Amanda; Martins,Maria de Fátima Monteiro; Viani,Flávio Cesar; Bondan,Eduardo Fernandes.
ABSTRACT Pro-inflammatory cytokines and glial cells, especially microglial cells, have been implicated in persistent pain sensitization. Less is known about the role of astrocytes in pain regulation. This study aimed to observe the expression of the astrocytic biomarker glial fibrillary acidic protein (GFAP) and the serum levels of interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) after short-term administration of central pain relievers in rats not submitted to noxious stimuli. Male Wistar rats were divided into five groups, receiving for nine days- (1) amitriptyline (Amt-10 mg/kg/day, by gavage); (2) gabapentin (Gb-60 mg/kg/day, by gavage; (3) methadone (Me-4.5 mg/kg/day, intraperitoneal route [IP]); (4) morphine (Mo-10 mg/kg/day, IP);...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuropathic pain/treatment; Astrocytes/pain regulation; Analgesics/tests/treatment; Cytokines; Glial Fibrillary Acidic Protein/study.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400623

Association between diet and polymorphisms in individuals with statin-controlled dyslipidaemia grouped according to oxidative stress biomarkers
Provedor de dados: 60
Autores: Botelho,Patrícia Borges; Fioratti,Cyntia Okoshi; Rogero,Marcelo Macedo; Barroso,Lucia Pereira; Bertolami,Marcelo Chiara; Castro,Inar Alves.
The objective of this study was to investigate whether differences in diet and in single-nucleotide polymorphisms (SNPs) found in paraoxonase-1 (PON-1), 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), cholesterol ester transfer protein (CETP) and apolipoprotein E (APOE) genes, are associated with oxidative stress biomarkers and consequently with susceptibility of low-density cholesterol (LDL) to oxidation. A multivariate approach was applied to a group of 55 patients according to three biomarkers: plasma antioxidant activity, malondialdehyde and oxidized LDL (oxLDL) concentrations. Individuals classified in Cluster III showed the worst prognoses in terms of antioxidant activity and oxidative status. Individuals classified in Cluster I presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diet; Oxidative stress/biomarkers; Single-nucleotide polymorphism (SNPs); Lipoproteins/oxidation; Statins; Apolipoprotein E.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100005

Stability-indicating HPLC-DAD method for the simultaneous determination of fluoroquinolone in combination with a non-steroidal anti-inflammatory drug in pharmaceutical formulation
Provedor de dados: 60
Autores: Araujo,Elaine de Oliveira; Rosa,Aline Marques; Amaral,Marcos Serrou do; Sversut,Rúbia Adrieli; Baroni,Adriano César de Morais; Oliveira,Lincoln Carlos Silva de; Kassab,Nájla Mohamad.
We developed and validated a stability-indicating assay method for the simultaneous determination of enrofloxacin and piroxicam in combination and in the presence of degradation products. Reverse-phase high-performance liquid chromatography analyses were carried out on a Vertisep C18 column and acetonitrile-water (48:52 v/v, pH 3.0) mobile phase with a 1.00 mL min−1 flow rate. The efficient chromatographic separation of these drugs and their forced degradation products was achieved in less than 5min with a peak purity match factor higher than 950. The method used showed linearity in the concentration ranges of 0.25 to 16.0 µg mL−1 for enrofloxacin (r = 0.9997) and 0.125 to 8.0 µg mL−1 for piroxicam (r = 0.9999) as well as precision (relative standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enrofloxacin; HPLC; Piroxicam; Simultaneous determination; Stability-indicating method.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100515

Midazolam and haloperidol for palliative sedation: physicochemical stability and compatibility of parenteral admixtures
Provedor de dados: 60
Autores: Estan-Cerezo,Gabriel; Rodríguez-Lucena,Francisco José; Chirivella,Carmen Matoses; Jiménez-Pulido,Inmaculada; García-Monsalve,Ana; Navarro-Ruiz,Andrés.
This study was done to determine the time while the binary admixtures with midazolam and haloperidol drugs are administered by perfusion to the patients in the clinical routine. Samples with different concentrations of both drugs were prepared following the usual clinical practice. Solvents used were 0.9 % sodium chloride solution and 5% dextrose, and viaflo plastic bags were used as the containers of the admixtures. Samples were not protected from light and were stored at 20 ºC or at 4 ºC. Compatibility and physicochemical stability were studied by visual inspection, turbidity measurement, pH determination and ultraviolet detection high performance liquid chromatography (UV-HPLC) was used to determine midazolam and haloperidol concentrations. The assay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Midazolam/pharmacology; Haloperidol/pharmacology; HPLC/methods; Additives in Sanitizing Products; Chemical stability; Physical stability; Physicochemical properties.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100524

Profile of patients with rheumatic diseases undergoing treatment with anti-TNF agents in the Brazilian Public Health System (SUS), Belo Horizonte - MG
Provedor de dados: 60
Autores: Oliveira Junior,Haliton Alves de; Almeida,Alessandra Maciel; Acurcio,Francisco Assis; Santos,Jéssica Barreto dos; Kakehasi,Adriana Maria; Alvares,Juliana; Dabés,Clarice Gomes e Souza; Cherchiglia,Mariangela Leal.
The aim of this study was to describe the baseline demographic and clinical characteristics as well as the functional status of a prospective cohort of patients with rheumatic diseases assisted by the Brazilian Public Health System (SUS). Data for 302 patients receiving tumor necrosis factor α inhibitors (anti-TNF agents) was collected through a standard form. Among patients, 229 (75.8%) were female and 155 (51.3%) were Caucasian; the mean age was 50.3 ± 12.8 years, and the mean disease duration was 9.9 ± 8.7 years. Among them 214 patients (70.9%) received adalimumab, 72 (23.8%) etanercept, and 16 (5.3%) infliximab. Mean Health Assessment Questionnaire-Disability Index (HAQ-DI) was 1.37 ± 0.67 for all participants. Poor functional response was associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatic diseases/treatment/patients profile; Rheumatoid arthritis; Ankylosing spondylitis; Psoriatic arthritis; Brazilian public health system; Anti-TNF agents/treatment of rheumatic diseases.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300709

Assessment of an anti-ageing structured cosmetic formulation containing goji berry
Provedor de dados: 60
Autores: Leite,Fernanda de Godoy; Oshiro Júnior,João Augusto; Chiavacci,Leila Aparecida; Chiari-Andréo,Bruna Galdorfini.
Based on previous studies, it has been found that goji berry (GB), popularly known as a ‘miracle fruit’, has excellent antioxidant potential and can be used in the treatment of skin disorders associated with ageing. This study aimed to incorporate GB into a structured cosmetic in order to optimise its penetration. Stability studies of the formulation, determination of the antioxidant activity of the extract and of the formulation, rheological measurements, SAXS, polarised light microscopy and bioadhesion analyses were performed. The results indicated the antioxidant activity of the extract, which can be incorporated into an emulsified cosmetic formulation. The emulsified formulation containing the extract remained stable, even after being submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goji Berry; Skin; Formulation; Lycium/drug effects; Skin Aging/immunology; Cosmetics/pharmacokinetics; Antioxidants/pharmacokinetics; Cosmetic Technology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100584

Development of mesalazine pellets coated with methacrylic-derived polymer
Provedor de dados: 60
Autores: Déo,Simone Cristina; Andreazza,Itamar Francisco; Possamai,João Carlos.
Mesalazine (5-ASA) is the standard drug for the treatment of inflammatory bowel disease (IBD) due to its local effect on intestinal and colonic mucosa. The effective and safe treatment of this disease requires more efficient delivery of the active substance to its site of action. The focus of this study was the use of multiparticulate systems, a modified release form in which the drug is divided into several functional subunits of release in the form of granules or pellets. When these forms are administered, they are rapidly disintegrated, distributing their content throughout the gastrointestinal tract. The aim of this study was to develop and evaluate a multiparticulate system consisting of pellets coated with polymer for pH-dependent release, derived...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesalazine; Coated pellets/evaluation; Coated pellets/dissolution profile; Extrusion- Spheronisation/pellets obtention; Drugs/modified release.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100013

Evaluation of (anti)genotoxic activities of Phyllanthus niruri L. in rat bone marrow using the micronucleus test
Provedor de dados: 60
Autores: Queiroz,Fernando Márlisson de; Matias,Kayo Wanderson de Oliveira; Cunha,Mylena Mylana Freire da; Schwarz,Aline.
Phyllanthus niruri L. (Euphorbiaceae), known as "quebra-pedra" (Portuguese for "stonebreaker"), is an herb used for kidney disorders. In light of its frequent use by the population, the present study aimed to investigate the genotoxic, antigenotoxic and cytotoxic activities of a standardized P. niruri extract in bone marrow rats. Three groups of 12 animals were treated daily by gavage over a period of 30 days, with 50, 150 or 250 mg/kg of P. niruri extract aqueous solution. The control group (n = 12) received tap water. At the end of treatment (day 31), groups were divided into two minor subgroups (n=6/group) and received cyclophosphamide (50 mg/kg, i.p.) or saline 0.9% (i.p.). After 24 hours, we evaluated the frequency of micronucleated polychromatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phyllanthus niruri L./genotoxicity; Phyllanthus niruri L./toxicological analysis; Phyllanthus niruri L./experimental study; Medicinal plantas/evaluation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100015

Effect of daidzein on Parkinson disease induced by reserpine in rats
Provedor de dados: 60
Autores: Goel,Radha; Chaudhary,Rabia.
Parkinson disease is a neurodegenerative disorder characterised by the cardinal symptoms of stiffness, resting tremor, slowness (bradykinesia) and reduction of movement (hypokinesia). Involvement of oxidative damage has been reported in the pathophysiology of Parkinson disease and its related complications. The purpose of this study was to examine the effect of daidzein to quench the free radicals produced as a result of the increased oxidative stress in Parkinson disease.Parkinson disease is induced by administration of reserpine (5 mg/kg/day, i.p) for 5 consecutive days. The symptoms of PD such as tremors, akinesia and rigidity were evaluated. The effect was evaluated by assessing various behavioral parameters (grip strength and locomotor activity),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daidzein; Reserpine; Carbidopa and Levodopa combination; Parkinson.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100559

Thermodynamic study of the solubility of ibuprofen in acetone and dichloromethane
Provedor de dados: 60
Autores: Aragón,Diana Marcela; Rosas,Jaiver Eduardo; Martínez,Fleming.
Thermodynamic functions, Gibbs energy, enthalpy and entropy for the solution processes of ibuprofen (IBP) in acetone and dichloromethane (DCM) were calculated from solubility values obtained at temperatures ranging from 293.15 K to 313.15 K. The respective thermodynamic functions for mixing and solvation processes as well as the activity coefficients for the solute were calculated. IBP solubility was high and proved similar in both solvents but was greater in DCM than acetone. In addition, the thermodynamic quantities for the transfer process of this drug from cyclohexane to the organic solvents were also calculated in order to estimate the contributions of hydrogen-bonds or of other dipolar interactions. The results were discussed in terms of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ibuprofen; Solubility; Transfer; Solution thermodynamics; Organic solvents.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200009

Alginate/hydrophobic HPMC (60M) particulate systems: new matrix for site-specific and controlled drug delivery
Provedor de dados: 60
Autores: Ghosal,Kajal; Ray,Sarbani Dey.
This study aimed to obtain site-specific and controlled drug release particulate systems. Some particulates were prepared using different concentrations of sodium alginate (Na-Alg) alone and others were formulated using different proportions of Na-Alg with hydroxypropyl methylcellulose (HPMC) stearoxy ether (60M viscosity grade), a hydrophobic form of conventional HPMC, using diclofenac potassium (DP) by ion-exchange methods. Beads were characterized by encapsulation efficiency, release profile, swelling, and erosion rate. The suitability of common empirical (zero-order, first-order and Higuchi) and semi-empirical (Ritger-Peppas and Peppas-Sahlin) models was studied to describe the drug release profile. The Weibull model was also studied. Models were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drugs/release particulate systems; Drugs/release profile; Hydroxypropyl methylcellulose stearoxy ether; Sodium alginate; Direct fitting; Case II transport; Beads/characterization; Beads/release kinetics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400021

Schizophrenia: effects of aripiprazole in metabolic syndrome
Provedor de dados: 60
Autores: Silva,Deborah Galvão Coelho da; Kanazawa,Luiz Kae Sales; Vecchia,Débora Dalla.
Schizophrenia, in general, is characterized by severe and disabling mental alterations, characterized by the impairment of one’s mental, behavioral and social activities, developing certain clinical symptoms, relevant to the diagnosis. The drugs used for the reversion of the symptoms cause several adverse effects that affect the patient’s health and well-being, such as motor, endocrine and cardiovascular damages. For a long time, little was known about the origin and the treatment of schizophrenia, which has become a curiosity for science, originating countless researches and theories that are background for several treatments. It is known that alterations in dopaminergic pathways are related to the development of the symptoms of the disease, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schizophrenia; Pharmacotherapy; Antipsychotics; Aripiprazole; Metabolic Syndrome.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100552

Polymeric micelles and molecular modeling applied to the development of radiopharmaceuticals
Provedor de dados: 60
Autores: Grallert,Sibila Roberta Marques; Rangel-Yagui,Carlota de Oliveira; Pasqualoto,Kerly Fernanda Mesquita; Tavares,Leoberto Costa.
Micelles composed of amphiphilic copolymers linked to a radioactive element are used in nuclear medicine predominantly as a diagnostic application. A relevant advantage of polymeric micelles in aqueous solution is their resulting particle size, which can vary from 10 to 100 nm in diameter. In this review, polymeric micelles labeled with radioisotopes including technetium (99mTc) and indium (111In), and their clinical applications for several diagnostic techniques, such as single photon emission computed tomography (SPECT), gamma-scintigraphy, and nuclear magnetic resonance (NMR), were discussed. Also, micelle use primarily for the diagnosis of lymphatic ducts and sentinel lymph nodes received special attention. Notably, the employment of these diagnostic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals/development; Polymeric micelles/diagnostic application; Radioisotopes; Imaging diagnostic agents; Diagnostic techniques.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100002

Modulatory effect of baicalein on gene expression and activity of antioxidant enzymes in streptozotocin-nicotinamide induced diabetic rats
Provedor de dados: 60
Autores: Sarkar,Purabi; Nath,Kaberi; Banu,Sofia.
Oxidative stress plays the central role in the pathogenesis and progression of diabetic complications. The present study aims to investigate the beneficial effect of oral administration of flavone baicalein in streptozotocin-nicotinamide (STZ-NA) induced diabetic rats by measuring oxidative stress markers, antioxidant enzyme activities and expression analysis of antioxidant genes. Experimental diabetes was induced by a single intraperitoneal (i.p.) injection of STZ (55 mg /kg b.wt), 15 min after the i.p. administration of NA. At the end of the experimental period, thiobarbituric acid reactive substances (TBARS), activities of antioxidant enzymes and expression levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Streptozotocin/pharmacology; Baicalein/pharmacology; Nicotinamide/pharmacology; Glibenclamide; Diabetes Mellitus/ Experimental/prevention and control; Gene Expression/drug effects; Antioxidants/pharmacology; Glyburide/pharmacology; Rats.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100508

Development of fast dissolving tablets of flurbiprofen by sublimation method and its in vitro evaluation
Provedor de dados: 60
Autores: Shaheen,Nabeela; Zaman,Shahiq uz.
Flurbiprofen belongs to Biopharmaceutical Classification System (BCS) class II drugs which are poorly soluble in water. The objective of present research work was to prepare fast dissolving tablets of Flurbiprofen using varying concentrations of three different sublimating agents to improve the dissolution rate. Seven formulations were prepared containing different concentrations of camphor, ammonium bicarbonate and thymol as sublimating agent along with primogel as a superdisintegrant. Tablets were manufactured by direct compression method. The prepared tablets were evaluated for pre-compression and post-compression parameters result, For all formulations result was within official limits. FTIR studies revealed that there were no interactions between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flurbiprofen/dissolution; Fast dissolving tablets; Drug release; Sublimating agent; Superdisintegrant.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400605

Assessment of pharmaceutical services in HIV/AIDS health units in the city of Niterói, Brazil
Provedor de dados: 60
Autores: Piccoli,Nilo Jorge; Brito,Monique Araújo de; Castilho,Selma Rodrigues de.
ABSTRACT This study aimed to conduct an assessment of pharmaceutical services in HIV/AIDS in the city of Niteroi, Brazil, with emphasis on management. It was done a descriptive cross-sectional study, and the approach used was a normative assessment focused on quality. The indicators used were analyzed individually or grouped according to the components of pharmaceutical services. The assessment identified some good points, especially regarding the good availability of ARVs, and good guide patients in the use of these drugs, and also some problems such as low levels of compliance in relation to good dispensing practices criteria and storage and a high time for the distribution of medicines. The result was a degree of 50.3% compliance with the quality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmaceutical assistance/evaluation; Pharmaceutical assistance/Brazil; Brazilian public health system/assistance/HIV/AIDS.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200610

Chemical composition and in vitro antibacterial activity of essential oils from Murraya paniculata (L.) Jack (Rutaceae) ripe and unripe fruits against bacterial genera Mycobacterium and Streptococcus
Provedor de dados: 60
Autores: Silva,Flávia Fernanda Alves da; Fernandes,Cassia Cristina; Santiago,Mariana Brentini; Martins,Carlos Henrique Gomes; Vieira,Tatiana Manzini; Crotti,Antônio Eduardo Miller; Miranda,Mayker Lazaro Dantas.
This study aims to investigate chemical composition of essential oils from Murraya paniculata (L.) Jack (Rutaceae) ripe and unripe fruits and determine their in vitro antibacterial activity. Essential oils were extracted by hydrodistillation from Murraya paniculata (L.) Jack ripe and unripe fruits collected in the Cerrado, in Rio Verde, southwestern Goiás, Brazil. They were analyzed by gas chromatography with flame ionization detector (GC-FID) and by gas chromatography-mass spectrometry (GC-MS). Sesquiterpenes, which represent the most abundant class of compounds in oils, predominated in both ripe and unripe fruits. Major constituents of essential oils extracted from ripe fruits (RF-EO) were (-caryophyllene (21.3%), (-ylangene (13.3%), germacrene-D (10.9%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Murraya paniculata (L.) Jack; Rutaceae; Anti-streptococcal activity; Mycobacterium kansasii; Antibacterial agent; Antimycobacterial activity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100592

Cholesterol oxides inhibit cholesterol esterification by lecithin: cholesterol acyl transferase
Provedor de dados: 60
Autores: Pincinato,Eder de Carvalho; Moriel,Patricia; Abdalla,Dulcinéia Saes Parra.
Cholesterol oxides are atherogenic and can affect the activity of diverse important enzymes for the lipidic metabolism. The effect of 7β-hydroxycholesterol, 7-ketocholesterol, 25-hydroxycholesterol, cholestan-3β,5α,6β-triol,5,6β-epoxycholesterol, 5,6α-epoxycholesterol and 7α-hydroxycholesterol on esterification of cholesterol by lecithin:cholesterol acyl transferase (LCAT, EC 2.3.1.43) and the transfer of esters of cholesterol oxides from high density lipoprotein (HDL) to low density lipoproteins (LDL) and very low density lipoproteins (VLDL) by cholesteryl ester transfer protein (CETP) was investigated. HDL enriched with increasing concentrations of cholesterol oxides was incubated with fresh plasma as source of LCAT. Cholesterol and cholesterol oxides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterol/oxides; Cholesterol/acyl transferase; Lecithin; Protein/cholesteryl ester transfer; Lipoprotein/high density; Cholesterol/reverse transport.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300007

Antiulcer, wound healing and hepatoprotective activities of the seaweeds Gracilaria crassa, Turbinaria ornata and Laurencia papillosa from the southeast coast of India
Provedor de dados: 60
Autores: Senthil,Kulandhaisamy Arul; Murugan,Annappan.
Seaweeds have bioactive compounds of interest in the pharmaceutical industry. In India, seaweeds are used exclusively for phycocolloids production and have not yet received consideration as a dietary supplement. So, it has become imperative to explore the biomedical potential of seaweeds and promote their utilization as a functional food. The seaweeds Turbinaria ornata, Gracillaria crassa and Laurencia papillosa, collected from the Tuticorin coast of the Southeast coast of India and selected based on preliminary screening, were extracted with acetone and evaluated for antiulcer, wound healing and hepatoprotective activities. L. papillosa showed the highest level of gastric protection activity (81%) at 200 mg/kg, comparable to the standard drug ranitidine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seaweed/biomedical potential; Seaweed/antiulcer activity; Seaweed/wound-healing; Seaweed/hepatoprotective activity; Gracilaria crassa/biomedical potential; Turbinaria ornata/biomedical potential; Laurencia papillosa/biomedical potential.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400006

Anti-inflammatory and antinociceptive activities of methanolic extract from red seaweed Dichotomaria obtusata
Provedor de dados: 60
Autores: García Delgado,Neivys; Frías Vázquez,Ana Iris; Cabrera Sánchez,Hiran; Soto del Valle,Roberto Menéndez; Sierra Gómez,Yusvel; Suárez Alfonso,Ana María.
The aim of the present work was to investigate the anti-inflammatory and antinociceptive effects of methanolic extract from D. obtusata using classic models in mice (croton oil-induced ear edema and acetic acid-induced writhing) and a phospholipase A2 activity test. Qualitative analysis of the chemical composition of seaweed was also determined by extraction with solvents of increasing polarity and precipitation and color tests. Results of qualitative chemical study showed the presence of lactonic and phenolic compounds, reduced carbohydrates, other sugars, flavonoids, fatty compounds, triterpenes and steroids. The extract inhibited mouse ear edema in a dose-dependent manner with an efficacy higher than 90% and a mean effective dose of 4.87µg/ear, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dichotomaria obtusata/pharmacognosy; Dichotomaria obtusata/anti-inflammatory activity; Dichotomaria obtusata/antinociceptive activity; Mouse ear edema test; Writhing test.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100008

Design and evaluation of rhubarb total free anthraquinones oral colon-specific drug delivery granules to improve the purgative effect
Provedor de dados: 60
Autores: Wei,Hongwei; Chang,Jinhua; Liu,Pei; Li,Zhongsi; Miao,Guangxin; Liu,Xigang; Liu,Cuizhe; Zhang,Xiangrong.
Rhubarb is commonly used as a cathartic in Asian countries. However, researchers have devotedextensive concerns to the quality control and safety of rhubarb and traditional Chinese preparations composed of rhubarb due to the instable purgative effect and potential nephrotoxicity of anthraquinones. In this study, we aimed to prepare rhubarb total free anthraquinones (RTFA) oral colon-specific drug delivery granules (RTFA-OCDD-GN) to delivery anthraquinones to colon to produce purgative effect. RTFA-OCDD-GN were prepared using chitosan and Eudragit S100 through a double-layer coating process and the formulation was optimized. Continuous release studies were performed in a simulated gastric fluid (pH 1.2), followed by a small-intestinal fluid (pH 6.8) and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhubarb; Anthraquinones; Oral colon-specific drug delivery granules; Dissolution; Purgative activity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100500

Hyaluronic acid-coated nanoemulsions loaded with a hydrophobic ion pair of all-trans retinoic acid for improving the anticancer activity
Provedor de dados: 60
Autores: Tinoco,Letícia Márcia da Silva; Silva,Flávia Lidiane Oliveira da; Ferreira,Lucas Antônio Miranda; Leite,Elaine Amaral; Carneiro,Guilherme.
All-trans retinoic acid (ATRA) has been studied for the treatment of cancer, including leukemia and breast cancer. This work aims to develop nanoemulsions (NE) loaded with a hydrophobic ion pair (HIP) of all-trans retinoic acid (ATRA) and a lipophilic amine, stearylamine (SA), and coated with hyaluronic acid (HA) to enhance anticancer activity and reducing toxicity. Blank NE was prepared by spontaneous emulsification and optimized prior to HIP incorporation. NE-ATRA was electrostatically coated with different concentrations of HA. Incorporation of ATRA-SA led to monodisperse NE with small size (129 ± 2 nm; IP 0.18 ± 0.005) and positive zeta potential (35.7 ± 1.0 mV). After coating with 0.5 mg/mL HA solution, the mean diameter slightly increased to 158 ± 5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: All-trans-retinoic acid; Breast neoplasms.Drug delivery; Hyaluronic acid; Nanoemulsion.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400616

In vivo inhibitory effect of suberoylanilide hydroxamic acid combined with sorafenib on human hepatocellular carcinoma cells
Provedor de dados: 60
Autores: Hao,Dalin; Deng,Fang; Shi,Hong; Wang,Hongsheng; Xiao,Fubin; Sun,Chengxue; Xu,Yansong; Li,Peng.
The present study aimed to investigate the in vivo inhibitory effect of histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) combined with sorafenib on human hepatocellular carcinoma HCCLM3 cells. The nude mice transplanted with HCCLM3 cells were randomly divided into control, SAHA, sorafenib and SAHA+sorafenib groups. The nude mice in the later 3 groups were intragastrically administrated with SAHA (10 mg·kg-1·day-1), sorafenib (10 mg·kg-1·day-1) and SAHA (10 mg·kg-1·day-1) combined with sorafenib (10 mg·kg-1·day-1), respectively, for successive 20 days. Finally, the inhibition rate of tumor was measured. The expressions of MEK1/2, p-ERK1/2, Cyclin D1, Bcl-2, Bax, p53, MMP-2, MMP-9 and uPA in tumor tissues were determined. Results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suberoylanilide hydroxamic acid; HCCLM3; Inhibitory; In vivo.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100528

Effect of Ficus hispida L. on normal and dexamethasone suppressed wound healing
Provedor de dados: 60
Autores: Murti,Krishna; Lambole,Vijay; Panchal,Mayank.
Ethanolic extract of roots of Ficus hispida was investigated in normal and dexamethasone depressed healing conditions, using incision, excision and dead space wound models in albino rats. The root extract of Ficus hispida has shown the maximum breaking strength compared to control group. The rate of epithelialization and wound contraction in excision model was better as compared to control groups. There was significant increase in granulation tissue weight and hydroxyproline content in dead space model compared to control group. The antihealing effect of dexamethasone was also reverted by the administration of ethanolic extract of Ficus hispida in all the wound models .The results indicated that the root extract of Ficus hispida has a significant wound...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ficus hispida/pharmacognosy; Ficus hispida/ethanolic extract; Ficus hispida/wound healing activity; Wound healing/experimental study.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400023

Effect of cross-linked biodegradable polymers on sustained release of sodium diclofenac-loaded microspheres
Provedor de dados: 60
Autores: Saha,Avik Kumar; Ray,Sarbani Dey.
The objective of this study was to formulate an oral sustained release delivery system of sodium diclofenac(DS) based on sodium alginate (SA) as a hydrophilic carrier in combination with chitosan (CH) and sodium carboxymethyl cellulose (SCMC) as drug release modifiers to overcome the drug-related adverse effects and to improve bioavailability. Microspheres of DS were prepared using an easy method of ionotropic gelation. The prepared beads were evaluated for mean particle size, entrapment efficiency, swelling capacity, erosion and in-vitro drug release. They were also subjected to various studies such as Fourier Transform Infra-Red Spectroscopy (FTIR) for drug polymer compatibility, Scanning Electron Microscopy for surface morphology, X-ray Powder...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alginate beads/preparation; Alginate beads/evaluation; Drugs/sustained release; Interpenetrating network; Sodium diclofenac/microsphere; Sodium diclofenac/sustained release; Ionotropic gelation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400028

In vitro antimicrobial efficacy of a fixed-dose combination of RHZE against M. tuberculosis
Provedor de dados: 60
Autores: Agertt,Vanessa Albertina; Bonez,Pauline Cordenonsi; Mizdal,Caren Rigon; Rossi,Grazielle Guidolin; Flores,Vanessa da Costa; Campos,Marli Matiko Anraku de.
ABSTRACT The use of drugs in fixed-dose combination (FDC) is now recommended by the World Health Organization (WHO) due to the emergence of multidrug-resistant strains of Mycobacterium tuberculosis. FDC uses different drugs against tuberculosis (TB) in a single tablet for phase-intensive therapeutic intervention. This therapy aims to optimize treatment, to prevent inappropriate use of drugs, and to prevent the emergence of new resistant strains. This study aims to evaluate the susceptibility of clinical isolates of M. tuberculosis against rifampicin, isoniazid, ethambutol, and pyrazinamide. The antimicrobials were tested separately and in associations according to FDC. This was used for broth microdilution method, which was compared to the proportions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis/treatment/drug combination; Mycobacterium tuberculosis/drug resistance; Mycobacterium tuberculosis/susceptibility/fixed dose combination.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300575

Floating ability and drug release evaluation of gastroretentive microparticles system containing metronidazole obtained by spray drying
Provedor de dados: 60
Autores: Nohemann,Laís; Almeida,Marina Penteado de; Ferrari,Priscileila Colerato.
Abstract Gastroretentive floating microparticles were developed and evaluated for the controlled metronidazole delivery for treatment of gastric disease. Floating microparticles, varying in proportions of chitosan and hydroxypropyl methylcellulose or ethylcellulose, were obtained by spray drying. Floating microparticles were characterized by physicochemical and in vitro studies, according to their floating ability and drug delivery. Microparticles presented mean diameter from 1.05 to 2.20 µm. The infrared spectroscopy confirmed the drug encapsulation and showed no chemical linkage between microparticles components. X-ray diffraction showed changes in the drug`s solid state, from crystalline to amorphous, indicating partial drug encapsulation, due to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Floating microparticle; Chitosan; Ethylcellulose; Hydroxypropyl methylcellulose; Controlled drug delivery.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100611

What is the role of the pharmacist?: physicians' and nurses' perspectives in community and hospital settings of Santiago de Cuba
Provedor de dados: 60
Autores: Dupotey Varela,Niurka María; Oliveira,Djenane Ramalho de; Sedeño Argilagos,Caridad; Oliveros Castro,Kisvel; Mosqueda Pérez,Elisveidis; Hidalgo Clavel,Yelina; Sánchez Bisset,Nelly.
This study was carried out to understand the perceptions and expectations of the other health care professionals about pharmacists' role in primary health care centers and hospitals in Santiago de Cuba (Cuba). A cross-sectional descriptive study was conducted, applying a self-administered questionnaire to health care professionals. The sample included 763 professionals (40.9% physicians and 59.1 % nurses) from hospitals and primary health care clinics, chosen by random stratified sampling,. The most common activity performed by pharmacists was the distribution of medications or the drug supply management (51.5%) and adverse drug reactions reporting (29.4%), activities performed daily by pharmacists. Pharmaceutical care practice was considered as poor by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacists/professional functions; Pharmaceutical care; Interprofessional communication.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400007

Antidepressant and skeletal muscle relaxant effects of the aqueous extract of the Prosopis cineraria
Provedor de dados: 60
Autores: George,Mathew; Joseph,Lincy; Sharma,Abishika.
The aqueous leaves extract of Prosopis cineraria (AEPC) is used traditionally for the treatment of various CNS disorder. The purpose of this study was to evaluate the extract for antidepressant and skeletal muscle relaxant activity. The antidepressant effect of the extract was evaluated using Forced swim test (FST). The immobility periods of control and treated mice were recorded. The antidepressant-like effect of tested compound was compared to that of imipramine (15 mg/kg. p.o). Muscle relaxant property was studied using rotarod apparatus and total fall off time for standard and control group was recorded. Phytochemical screening revealed the presence of saponins, flavonoids, alkaloids, glycosides, tannins and phenolic compounds. The leaf extract at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proposis cineraria/pharmacognosy; Proposis cineraria/extract; Proposis cineraria/antidepressant effect; Proposis cineraria/extract; Proposis cineraria/relaxant property; Skeletal muscle; Imipramine/comparative study; Diazepam/comparative study.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300025

The effect of natural and organophilic palygorskite on skin wound healing in rats
Provedor de dados: 60
Autores: Silva,Mirna Luciano de Gois da; Fortes,Amanda Campos; Tomé,Adriana da Rocha; Silva Filho,Edson Cavalcanti da; Freitas,Rivelilson Mendes de; Soares-Sobrinho,José Lamartine; Leite,Cleide Maria da Silva; Soares,Mônica Felts de La Roca.
In view of growing interest in natural treatments, clays would appear to be a good alternative for speeding up the healing process during the treatment of wounds. Of the various clays, palygorskite, a clay from the Brazilian State of Piauí, composed of silicon and aluminum, has shown itself to be pharmaceutically useful as a healing agent. The aim of this article is to evaluate the effect on the healing of wounds of Piauí palygorskite, both in its natural state and when organophilic, by way of comparative analysis of macroscopic and histological tests on skin wounds in adult male and female two-month-old Wistar rats. To this end, a circular trichotomy of the dorsal cornus of the rats was carried out to confirm the effects of treatments involving 0.9%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attapulgite/healing agent; Palygorskite/healing agent; Healing/experimental study; Skin wounds/healing; Skin wounds/experimental study.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400012

Preliminary study to characterize differences in potential immunomodulatory effects of cyclosporine A using BALB/c and ICR mouse splenocytes
Provedor de dados: 60
Autores: Mariño,Angélica; Plaza,David; Delgado,Gabriela.
Cyclosporine A (CsA) is widely used as an immunosuppressant for the treatment of autoimmune diseases and immune regulation in transplant patients. Due to its wide applicability, studies of unwanted side effects of CsA are imperative. It has been found that not all patients treated with CsA display the same types/patterns of adverse effects. To ascertain the bases for these differential responses, potential differing effects of CsA on B-lymphocytes were analyzed. This entailed an assessment of changes in CsA viability and mitotic activity within splenocyte populations from BALB/c and ICR mice. These particular strains were examined because: (1) in each of them, previously have been shown that differed in the respond to biological response modifiers, such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunotoxicity; Cyclosporine A/immunomodulatory effects; Cyclosporine A/adverse effects; B-lymphocytes; BALB/c ICR; Blastogenesis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100022

In vitro biological activities of silver nanoparticles synthesized from Scedosporium sp. isolated from soil
Provedor de dados: 60
Autores: Panchangam,Rajeeva Lochana; Upputuri,Ravi Theaj Prakash.
One of the important fields in nanotechnology is the development of an environment friendly method for the synthesis of nanoparticles. Many approaches show that microorganisms are the most reliable tools for biosynthesis of nanoparticles compared to physical and chemical methods. In our study, fungi have been exploited for extracellular production of metal nanoparticles. It was observed that in Scedosporium, silver ions are reduced to silver nanoparticles, which was confirmed by UV-visible spectrophotometry and AFM. Optimization studies showed that as the concentration of AgNO3 used for synthesis increased, particles’ size also increased. Size of the particles at different concentrations of AgNO3 was observed to be 79-107 nm with particles being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atomic force microscopy; In vitro cytotoxicity; Scedosporium sp; Silver nanoparticles; UV-visible spectroscopy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100577

Evaluation of Lead and Copper content in hair of workers from oil product distribution companies in Iraq
Provedor de dados: 60
Autores: Jaccob,Ausama Ayob.
Serious health consequences can occur not only from acute exposure to environmental toxicants, but also from chronic exposures to minute quantities. Petroleum substances are widely distributed and used in huge amount, mainly as fuels. A total number of 45 males aged between 20 and 45 years participated in this study. The participants were divided in to two groups: The first group of 25 subjects worked at least 8hours/day in different oil product distribution companies in both Basrah and Thiqar Governorates-Iraq. The second group (control group) of 20 healthy individuals with age and weight matched with the test group and lived far away from hazardous exposure. Heavy metals levels in hair were measured by Atomic Absorption Spectrophotometer. Statistical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fuel; Heavy metals/effects; Hair; Pollutions.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100523

Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets
Provedor de dados: 60
Autores: Oliveira,Cristiani Lopes Capistrano Gonçalves de; Salgado,Hérida Regina Nunes; Moraes,Maria de Lourdes Leite.
A simple and fast alternative methodology using capillary zone electrophoresis (CZE) to analyze linezolid and its cationic photodegradation products in tablets has been developed. The separation was carried out on fused silica capillary and conducted using 100 mM formic acid (pH 3.0) and by applying 30 KV voltage. Detection was performed at UV 254 nm. The optimized method was validated in terms of linearity, limits of detection and quantification, precision (repeatability), stability studies (selectivity) and accuracy. Good linearity (8-20 mg L-1) was obtained and the limit of detection was 0.95 mg L-1. The greatest advantages of the CZE method were the rapid set-up of instrumentation and capillary equilibration, short analysis time (12 min), low running...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capillary-electrophoresis/assay; Linezolid/tablets/stability; Pharmaceutical-drugs.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400621

LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study
Provedor de dados: 60
Autores: Fontana,Márcia Camponogara; Laureano,João Víctor; Forgearini,Betielli; Chaves,Paula dos Santos; Araujo,Bibiana Verlindo de; Beck,Ruy Carlos Ruver.
A specific, precise, and accurate LC-UV method was developed and validated to assay raloxifene hydrochloride in rat plasma. Raloxifene was analyzed after liquid-liquid extraction and quantified by reversed phase liquid chromatography (C18 column) using acetonitrile and ammonium acetate buffer 0.05 M (pH 4.0) as mobile phase at a flow rate of 1 mL.min-1 and UV detection at 287 nm. Retention times of raloxifene and internal standard (dexamethasone) were approximately 11 min and 14 min, respectively. Linearity was checked for a concentration range between 25 ng.mL-1 and 1000 ng.mL-1. Intra- and inter-day precision had relative standard deviation lower than 10% and 15%, respectively. Recovery from plasma was higher than 90%. Accuracy values were 98.21%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raloxifene/pharmacokinetic; Liquid chromatography.; Plasma.; Bioavailability.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100538

Spray dried aqueous extract of Orthosiphon aristatus Blume (Java tea)
Provedor de dados: 60
Autores: Robaina-Mesa,Malvis; López-Hernández,Oreste Dario; Rodríguez-Chanfrau,Jorge Enrique; Nogueira-Mendoza,Antonio.
ABSTRACT Orthosiphon aristatus (Lamiaceae) is an herb medicinal found mainly in China, Indian and South East Asia. The purpose of this work was to develop a technological process for obtaining dry extract of Orthosiphon aristatus by spray dry. A process for the obtaining of dry extract from aqueous extract of Orthosiphon aristatus was studied. Response Surface Methodology experimental design was applied to evaluate the effects of inlet and outlet air temperature on drying yield (%). Mixture experimental design was applied to evaluate the drying adjuvant - total solids relation. Maltodextrin was evaluated as drying adjuvant. The best results were obtained when applying an inlet temperature of 120 °C and outlet temperature of 80 °C and a drying adjuvant -...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orthosiphon aristatus/extract; Spray dry/process; Drying adjuvant; Reflux method; Experimental design.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300608

Assay and physicochemical characterization of the antiparasitic albendazole
Provedor de dados: 60
Autores: Cavalcanti,Noely Camila Tavares; Sousa,Giovana Damasceno; Tabosa,Maria Alice Maciel; Soares Sobrinho,José Lamartine; Leal,Leila Bastos; Santana,Davi Pereira de.
The aim of this study was to characterize three batches of albendazole by pharmacopeial and complementary analytical techniques in order to establish more detailed specifications for the development of pharmaceutical forms. The ABZ01, ABZ02, and ABZ03 batches had melting points of 208 ºC, 208 ºC, and 209 ºC, respectively. X-ray diffraction revealed that all three batches showed crystalline behavior and the absence of polymorphism. Scanning electron microscopy showed that all the samples were crystals of different sizes with a strong tendency to aggregate. The samples were insoluble in water (5.07, 4.27, and 4.52 mg mL-1, respectively) and very slightly soluble in 0.1 M HCl (55.10, 56.90, and 61.70 mg mL-1, respectively) and additionally showed purities...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albendazole/characterization; Antiparasitics/quality control.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200012

Antioxidant, antimicrobial and phytotoxic activities of Rhaponticum acaule DC. essential oil
Provedor de dados: 60
Autores: Mosbah,Habib; Sassi,Ahlem Ben; Chahdoura,Hassiba; Snoussi,Mejdi; Flamini,Guido; Achour,Lotfi; Selmi,Boulbaba.
The in-vitro antioxidant activity of Rhaponticum acaule essential oil (RaEO) was evaluated using β-carotene/linoleic acid bleaching, chelating activity, and lipid peroxidation inhibition (TBARS) assays. The antimicrobial activity of RaEO was assessed by disc diffusion and microdilution methods against 8 bacteria and 4 yeast. Finally, the allelopathic activity of RaEO on the seed germination and the shoot and root elongation of lettuce (Lactuca sativa L.) seedlings were investigated. According to our results, the RaEO exhibited significant antioxidant activity, similar to those of standards (BHT and ascorbic acid) with IC50 values of 0.042 and 0.045 mg/mL obtained by β-carotene bleaching and TBARS assays, respectively. On the other hand, despite its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhaponticum acaule essential oil; Antioxidant activity; Antimicrobial activity; Allelopathic activity.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100611

Desenvolvimento e validação de método analítico em CLAE-UV para a quantificação de ácido retinóico em microcápsulas de alginato e quitosana
Provedor de dados: 60
Autores: Velloso,Fabiana Toledo; Ferraz,Rafaela Siqueira; Lira,Ana Amélia Moreira; Santana,Davi Pereira de; Santos-Magalhães,Nereide Stela.
O ácido retinóico (AR) tem sido utilizado para o tratamento de acne severa, rugas, estrias e celulite, no entanto, provoca irritação na pele e sofre rápida degradação quando exposto à luz e ao calor. Métodos analíticos rápidos para quantificação do AR são, portanto, necessários para ensaios de cinética de liberação in vitro. Nesse contexto, o objetivo deste trabalho foi desenvolver e validar um método rápido e sensível para o doseamento do AR em microcápsulas de alginato/quitosana contendo óleo de babaçu dispersas em gel natrosol® por cromatografia líquida de alta eficiência associada à espectroscopia UV e aplicá-lo na avaliação do perfil de liberação in vitro dessas formulações. As análises foram realizadas em modo isocrático utilizando coluna C18 de fase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cromatografia líquida de alta eficiência; Espectroscopia/ultra violeta; Ácido retinóico/quantificação; Microcápsulas/análise quantitativa; Formulações farmacêuticas/liberação in vitro.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100022

Anticonvulsant evaluation of 2-pyrazolines carrying naphthyl moiety: An insight into synthesis and molecular docking study
Provedor de dados: 60
Autores: Nusrat,Birjees; Siddiqui,Nadeem; Sahu,Meeta; Naim,Mohd. Javed; Shahar Yar,Mohammad; Ali,Ruhi; Ozair,Alam.
A series of N-substituted-3-(napthalen-2-yl)-5-substituted phenyl-4,5-dihydropyrazole-1-carbothioamide derivatives (4a-n) were synthesized with the view of structural requirements of pharmacophore for potential anticonvulsant agents. The synthesized compounds were assayed intraperitoneally (i.p.) and subcutaneously (s.c.) in mice against seizures induced by MES and scPTZ methods, respectively.Neurologic deficit was evaluated by rotarod method. Among the tested compounds, 4g, 4i, 4j and 4n emerged as the most active molecule in the MES model at a dose of 30 mg/kg at 0.5h comparable to standardscarbamazepine and phenytoin. In the scPTZ test,4e and 4l were found to be most active compounds at the lowest dose of 30 mg/kg at 0.5h, in the management of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrazolines; Anticonvulsants; Molecular docking; Neurotoxicity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100516

Computational drug discovery for the Zika virus
Provedor de dados: 60
Autores: Mottin,Melina; Borba,Joyce Villa Verde Bastos; Melo-Filho,Cleber Camilo; Neves,Bruno Junior; Muratov,Eugene; Torres,Pedro Henrique Monteiro; Braga,Rodolpho Campos; Perryman,Alexander; Ekins,Sean; Andrade,Carolina Horta.
Few Zika virus (ZIKV) outbreaks had been reported since its first detection in 1947, until the recent epidemics occurred in South America (2014/2015) and expeditiously became a global public health emergency. This arbovirus reached 0.5-1.3 million cases of ZIKV infection in Brazil in 2015 and rapidly spread in new geographic areas such as the Americas. Despite the mild symptoms of the Zika fever, the major concern is related to the related severe neurological disorders, especially microcephaly in newborns. Advances in ZIKV drug discovery have been made recently and constitute promising approaches to ZIKV treatment. In this review, we summarize current computational drug discovery efforts and their applicability to discovery of anti-ZIKV drugs. Lastly, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika; Flavivirus Antiviral; Computer-assisted drug design; Virtual screening; Drug discovery.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700401

Antioxidant and immunomodulatory activities of Oviductus ranae in mice
Provedor de dados: 60
Autores: Zhang,Yang; Liu,Yang; Yang,Xiudong; Cui,Hao; Xu,Xiaoxiao; Mao,Liping; Zhou,Hongli.
Oviductus ranae (OR) is a traditional Chinese medicine, which was first recorded in the Compendium of Materia Medica in the Ming Dynasty. OR contains high amounts of proteins and elicits therapeutic effects on neurasthenia, insomnia, and respiratory symptoms, which are related to oxidative stress and immunodeficiency. This study aimed to obtain the potential of OR for the development of functional food possessing antioxidant and immune-enhancement functions in the same dose. In antioxidant evaluation, OR can significantly decrease malondialdehyde and protein carbonyls (P &lt; 0.05, P &lt; 0.01) and significantly increase total superoxide dismutase and glutathione in a dose-dependent manner (P&lt; 0.05, P &lt; 0.01) against ethanol-induced oxidative stress...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oviductus ranae/evaluation/antioxidant activity; Immunomodulatory activity; Functional food.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400626

Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
Provedor de dados: 60
Autores: Ren,Zhouxin; Shen,Junling; Mei,Xiaofeng; Dong,Haoran; Li,Jiansheng; Yu,Haibin.
Hesperidin, a natural compound, suppresses the epithelial-to-mesenchymal transition through the TGF-β1/Smad signaling pathway. However, studies on the detailed effects and mechanisms of hesperidin are rare. The present study showed that, for A549 alveolar epithelial cells, the anti-proliferative effects of hesperidin occurred in a dose-dependent manner, with an IC50= 216.8 µM at 48 h. TGF-β1 was used to activate the Smad signaling pathway and induce the epithelial to mesenchymal transition in cells. Treatment with hesperidin or SB431542 was used for antagonism of Smad pathway activation. Hesperidin inhibited the increase in ɑ-SMA and Col1ɑ-1 and the decrease in E-cadherin in a dose-dependent manner from concentration of 20 µM to 60 µM, as assessed by both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hesperidin; Epithelial-to-mesenchymal transition (EMT); Smad signaling pathway; Inhibition.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100566

Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA
Provedor de dados: 60
Autores: Rodrigues,Caroline Danziato; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.
In this work, we developed and validated an effective reversed-phase HPLC method with photodiode array (PDA) detection for the quantitative analysis of amphotericin B (AmB) in poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles. Chromatographic runs were performed on a reverse phase C18 column using a mobile phase comprising a 9% acetic acid and acetonitrile mixture (40:60, v/v) under isocratic elution with a flow rate of 1 mL/min. AmB was detected at a wavelength of 408 nm. The validation process was performed considering the selectivity, linearity, precision, accuracy, robustness, limit of detection (LOD) and limit of quantitation (LOQ) of the method. A concentration range of 1-20 µg/mL was used to construct a linear calibration curve. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphotericin B/determination; Amphotericin B/encapsulation; High performance liquid chromatography/reverse phase/quantitative analysis; Nanoparticles.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400859

Synthesis and cytotoxicity evaluation of thiosemicarbazones and their thiazole derivatives
Provedor de dados: 60
Autores: Braga,Saulo Feheiberg Pinto; Fonseca,Nayara Cristina; Ramos,Jonas Pereira; Souza-Fagundes,Elaine Maria de; Oliveira,Renata Barbosa de.
ABSTRACT The aims of this study were to synthesize a series of thiosemicarbazones and their thiazole derivatives, to investigate their cytotoxic activity against three human cancers and normal (Vero cells) cell lines, and to evaluate the pro-apoptotic potential of the most active compounds. Materials and Methods: The thiosemicarbazones were obtained by reacting an aromatic aldehyde with thiosemicarbazide (yield 71-96%), which were subjected to a cyclization with α-bromoacetophenone to yield the required thiazole heterocycles (yield 63-100%). All the synthesized compounds were screened at 50 µM concentration against three cell lines representing HL60 (promyelocytic leukemia), Jurkat (acute lymphoblastic leukemia), and MCF-7 (breast cancer). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thiosemicarbazones/synthesis; Thiosemicarbazones/cytotoxic activity; Thiazoles/derivatives/synthesis; Thiazoles/derivatives/cytotoxic activity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200299

Formulation and in vitro evaluation of fast dissolving tablets of metoprolol tartrate
Provedor de dados: 60
Autores: Satpute,Mangesh Machhindranath; Tour,Nagesh Shivaji.
The demand for fast dissolving tablets has been growing during the last decade, especially for elderly and children who have swallowing difficulties. In the present work, fast dissolving tablets of metoprolol tartrate, were prepared using sodium starch glycolate, sodium croscarmellose and crospovidone as superdisintegrants, by the direct compression method. The tablets prepared were evaluated for various parameters including weight variation, hardness, friability, in vitro dispersion time, drug-polymer interaction, drug content water absorption ratio, wetting time, in vitro drug release, FTIR and DSC studies. The tablets prepared by the direct compression method had a weight variation in the range of 145 mg to 152 mg, which is below ± 7.5%, a hardness of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fast dissolving tablets/in vitro evaluation; Fast dissolving tablets/formulation; Metoprolol tartrate/fast dissolving tablets; Metoprolol tartrate/formulation; Metoprolol tartrate/in vitro evaluation; Sodium croscarmellose; Sodium starch glycolate; Crospovidone; In vitro dispersion time/tablets evaluation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400018

Simultaneous determination of abamectin homologs H 2 B 1a and H 2 B 1b in gel formulation by high performance liquid chromatography
Provedor de dados: 60
Autores: Alexandre,Grazielle Prado; Aurora-Prado,María Segunda; Español Mariño,Laura Victoria; Singh,Anil Kumar; Leite,Helen Dutra; Kedor-Hackmann,Erika Rosa Maria; Santoro,Maria Inês Rocha Miritello.
ABSTRACT Abamectin is a drug with antiparasitic properties used in several pharmaceutical formulations. The objective of this research was to develop and validate a high performance liquid chromatographic (HPLC) method for quantification of the two abamectin homologs (H2B1a and H2B1b) in gel formulation. This HPLC method was validated using a LichroCart(r) 100 RP-18 (125 x 4 mm, 5 µm) column. The mobile phase contained of acetonitrile and water (95:5 v/v) with 1% acetic acid. The flow rate was 1.0 mL min-1 and UV detection was performed at 245 nm. Mobile phase solutions were prepared containing a nominal concentration 185.2 µg mL-1 H2B1a and 9.6 µg mL-1 H2B1b. The method displayed good linearity in the concentration range of 148.1 - 222.3 µg mL-1 and 7.7 -...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abamectin homologs/validation method/gel formulation; Abamectin homologs; High Perfomance Liquid Chromatography (HPLC).
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300509

Development and validation of a new and economical stability indicating RP-HPLC method for cefixime trihydrate
Provedor de dados: 60
Autores: Mathew,Ceema; Swathi,Bellamkonda; Ajitha,Makula; Babu,Puvvadi Sathesh.
ABSTRACT The present work describes the development of a new high performance liquid chromatographic (HPLC) method for the determination of Cefixime trihydrate under different stress conditons as specified by ICH. For the analysis, a Phenomenex (250 x 4.6 mm, 5 µm particle size) ODS column and a SPD 20 A UV detector at 289 nm was used. The selected mobile phase was 10 mM disodium hydrogen phosphate (with 0.5% TEA, pH adjusted to 6.3 with OPA) and methanol in the ratio of 75:25 (v/v) in isocratic mode at a flow rate of 1 mL.min-1.The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9997 in the concentration range of 5-100 μg.mL-1. The stress degradation was performed using acid, alkali, water, hydrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High Performance Liquid Chromatography/quantitative analysis; Degradation; Stability-indicating; Cefixime trihydrate/determination.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100010

Influence of different cosmetic vehicles in mechanical and physical properties of hair treated with oxidative hair dyes
Provedor de dados: 60
Autores: Serrão,Elaine Cabral; Gama,Robson Miranda da; Dario,Michelli Ferrera; França-Stefoni,Simone Aparecida da; Bedin,Valcinir; Baby,André Rolim; Velasco,Maria Valéria Robles.
ABSTRACT Hair care products play a significant role in the cosmetic market and aim at improving hair brightness, breakage resistance, and color change. In this study, we analyzed the possibility of the formulation of oxidative dyes in different vehicles impacting the hair’s both mechanical and physical properties. Light brown and light blond dyes were prepared using eight different vehicles. Among these, four vehicles were emulsifying agents and four gelling agents. Each formulation was applied to two types of virgin Caucasian hair (light blond and dark brown). Physical, chemical, and organoleptic properties of each formulation were assessed, as well as changes in hair parameters after oxidative dyeing, such as staining intensity, brightness, and breaking...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hair dyes/analyse; Cosmetic technique/utilization; Hair/chemistry; Hair color; Hair/strength; Hair/bright.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100611

Icariin stimulates differentiation of bone marrow-derived mesenchymal stem cells (BM-MSCs) through activation of cAMP/PKA/CREB
Provedor de dados: 60
Autores: Lou,Dan; Ye,Jifeng; Yang,Lianhua; Wu,Zheng; Zheng,Wei; Zhang,Hui.
Icariin, a prenylated flavonol glycoside isolated from Epimedium, has been considered as a potential alternative therapy for osteoporosis. The present study aimed to clarify the detailed molecular mechanisms of action of icariin on osteoblast function, using bone marrow-derived mesenchymal stem cells (BM-MSCs). BM-MSCs were first stimulated by icariin. Then, gene and protein expression of cAMP/PKA/CREB signaling molecules were analyzed by RT-PCR and western blotting (WB), and alkaline phosphatase (ALP) was analyzed in cell lysates by ELISA. MTT assays indicated that icariin did not have significant effects on cell viability up to 1 µM. Icariin showed a dose-dependent effect on the alkaline phosphatase activity of BM-MSCs. WB analysis showed that icariin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Icariin/molecular mechanisms; BM-MSCs; CAMP; PKA; CREB.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100592

Pharmacoeconomy: an indispensable tool for the rationalization of health costs
Provedor de dados: 60
Autores: Areda,Camila Alves; Bonizio,Roni Cléber; Freitas,Osvaldo de.
Due to the considerable increase in public expenditure with health issues, mainly regarding drugs, several countries, including Australia and England, have already implemented, or are in the process of discussing the adoption of measures to ensure the quality of health care provided to the population. One of the less harmful strategies, rarely used in Brazil, is the adoption of economic techniques applied to health, more specifically, pharmacoeconomic analysis. This paper aims to contribute to the dissemination of concepts and techniques of economic analysis with a view to incorporate these into policy decisions of expenditure rationalization and the search for clinical efficiency. It includes a literature review covering the types of costs and benefits in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacoeconomy; Pharmacoeconomics analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200004

Evaluation of the functionality of bread loaves prepared with quinoa flakes through biological tests
Provedor de dados: 60
Autores: Gewehr,Márcia Flach; Pagno,Carlos Henrique; Danelli,Daiane; Melo,Lívia Marchi de; Flôres,Simone Hickmann; Jong,Erna Vogt de.
ABSTRACT The aim of this study was to evaluate quinoa flakes and bread prepared with the pseudocereal with regard to nutritional aspects by determining chemical composition, content of tocopherols and fatty acid composition as well as to possible beneficial effects in Wistar rats given commercial feed supplemented or not with quinoa flakes and cholesterol. The following parameters were determined: weight gain, food intake, liver weight, fat in liver and lipid profile in blood and liver. The results showed that processing affected the nutritional characteristics of bread because there was a significant increase in dietary fiber content but reduction in soluble fiber. Increased fiber did not influence food intake or weight gain. Regarding fat in liver of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quinoa flakes/nutritional evaluation; Quinoa flakes/beneficial effects; Composition; Bread/processing/effects; Bread/ nutritional evaluation; Tocopherols.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200337

Food analysis
Provedor de dados: 60
Autores: Colli,Célia; Lobo,Alexandre Rodrigues.
Tipo: Info:eu-repo/semantics/review
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100028

Design, synthesis, biological evaluation, and nitric-oxide release studies of a novel series of celecoxib prodrugs possessing a nitric-oxide donor moiety
Provedor de dados: 60
Autores: Soliman,Wael Mohamed; Abdellatif,Khaled Rashad Ahmed; Knaus,Edward Elmer.
A new group of hybrid nitric oxide-releasing anti-inflammatory drugs (NONO-coxibs), in which an O 2-acetoxymethyl-1-(N-ethyl-N-methylamino)diazen-1-ium-1,2-diolate NO-donor moiety is attached directly to the carboxylic acid group of 1-(4-aminosulfonylphenyl)-5-aryl-1H-pyrazol-3-carboxylic acids (6a-c), were synthesized. A low amount of NO was released from the diazen-1-ium-1,2-diolate compounds 6a-c upon incubation with phosphate buffer saline (PBS) at pH 7.4 (range: pH 7.97-8.51), whereas, the percentage of NO released was significantly higher (84.5%-85.05% of the theoretical maximal release of two molecules of NO/molecule of the parent hybrid ester prodrug) when the diazen-1-ium-1,2-diolate ester prodrugs were incubated in the presence of rat serum....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-inflammatory/development/drugs NONO-coxib esters 1; 5-dihydropyrazoles.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400612

Cytotoxicity and DNA damage in the neutrophils of patients with sickle cell anaemia treated with hydroxyurea
Provedor de dados: 60
Autores: Pedrosa,Alano Martins; Barbosa,Maritza Cavalcante; Santos,Thayna Nogueira dos; Leal,Luzia Kalyne Almeida Moreira; Lopes,Amanda de Araújo; Elias,Darcielle Bruna Dias; Sasahara,Greyce Luri; Cavalcanti,Bruno Coêlho; Gonçalves,Romélia Pinheiro.
Hydroxyurea (HU) is the most important advance in the treatment of sickle cell anaemia (SCA) for preventing complications and improving quality of life for patients. However, some aspects of treatment with HU remain unclear, including their effect on and potential toxicity to other blood cells such as neutrophils. This study used the measurement of Lactate Dehydrogenase (LDH) and Methyl ThiazolTetrazolium (MTT) and the comet assay to investigate the cytotoxicity and damage index (DI) of the DNA in the neutrophils of patients with SCA using HU.In the LDH and MTT assays, a cytoprotective effect was observed in the group of patients treated, as well as an absence of toxicity. When compared to patients without the treatment, the SS group (n=20, 13 women and 07...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxyurea/cytotoxicity; Sickle cell anaemia/treatment; Neutrophils/DNA damage; Deoxyribonucleic acid/damage.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200401

Stability of hydroalcoholic extracts of two species of guaco; Mikania glomerata SPRENG. and Mikania laevigata SCHULTZ. (Asteraceae), by UHPLC-MS
Provedor de dados: 60
Autores: Melo,Lucilia Vilela de; Sawaya,Alexandra Christine Helena Frankland.
It is important to study the stability of plant extracts used as active ingredients in phytotherapic medicine, as degradation of the active principles directly affects the efficacy and safety of these products. Therefore, a stability study of the hydroalcoholic extract of the species: Mikania glomerata and Mikania laevigata was conducted in order to determine the speed of degradation and shelf life of these extracts, which are incorporated in cough syrup in Brazil. Leaves of both species were dried in an oven or by lyophilization (freeze-dried). Hydroalcoholic extracts underwent both accelerated stability study of six months and long-term stability study for 12 months. Samples were stored at different temperatures and every three months were analysed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mikania glomerata Spreng.; Mikania laevigata Sch; Bip ex Baker; Guaco; Ultra-high performance liquid chromatography-mass spectrometry; Stability test; Coumarin; Chlorogenic acid.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100584

Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis
Provedor de dados: 60
Autores: Chelladurai,Gladis Raja Malar; Chinnachamy,Chellaram.
ABSTRACT Our present investigation deals with the phytochemical screening, estimation of total flavonoids, terpenoids and tannin contents to evaluate the anti-diabetic activities of Salacia oblonga stem followed by GC-MS analysis. It explores the natural compounds and the potential α-amylase and α-glucosidase inhibitory actions of stem extracts. The aqueous stem extract was selected from other extracts (ethanol, acetone, petroleum ether and chloroform) for the in vitro study of anti-diabetic activity by alpha amylase and alpha glucosidase inhibitory assays. The stem extract was also analyzed by gas chromatography mass spectrometry to identify the natural chemical components. Phytochemical analysis of aqueous stem extract showed major classes of secondary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salacia oblonga; Antidiabetic; Α-Amylase; Α-Glucosidase; Terpenoids.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100606

The effect of food intake on the pk of rhein released from diacerein
Provedor de dados: 60
Autores: Huang,Ming; Zong,Shun-lin; Zhang,Quan-ying.
Diacerein is a symptomatic slow-acting drug used for treating osteoarthritis. This drug is completely metabolized into the active metabolite rhein before reaching the systemic circulation. This study evaluated the effects of food on the pharmacokinetics of rhein released from diacerein in healthy Chinese subjects. This was a single-center, randomized, single-dose, open-label, two-period, cross-over study. Twenty-four healthy subjects were randomly selected to receive a single oral dose of 50 mg diacerein capsule in either fasted or fed state on two separate visits. Plasma samples were analyzed with LC-MS/MS. Pharmacokinetic parameters were calculated using WinNonlin software. In the fasted and fed states, the main pharmacokinetic parameters of diacerein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diacerein capsule/pharmacokinetics; HPLC-MS/MS; High fat and calories diet.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100544

Determination of parabens in sweeteners by capillary electrochromatography
Provedor de dados: 60
Autores: Bottoli,Carla Beatriz Grespan; Gutiérrez-Ponce,María de Jesús Santa; Aguiar,Valeska Soares; Aquino,Walkyria Moraes de.
Parabens, common food preservatives, were analysed by capillary electrochromatography, using a commercial C18 silica (3 µm, 40 cm × 100 µm i. d.) capillary column as separation phase. In order to optimise the separation of these preservatives, the effects of mobile phase composition on the separation were evaluated, as well as the applied voltage and injection conditions. The retention behavior of these analytes was strongly influenced by the level of acetonitrile in the mobile phase. An optimal separation of the parabens was obtained within 18.5 minutes with a pH 8.0 mobile phase composed of 50:50 v/v tris(hydroxymethyl)aminomethane buffer and acetonitrile. The method was successfully applied to the quantitative analysis of paraben preservatives in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capillary eletrochromatography/quantitative analysis; Parabenos/determination; Sweeteners/quantitative analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400015

Effect of MDR C3435T polymorphism on Varenicline treatment in quit smoking
Provedor de dados: 60
Autores: Bozkurt,Nurgul; Altıntas,Fatih; Bozkurt,Ali Ihsan; Turgut,Gunfer; Turgut,Sebahat.
Despite so many global efforts, smoking still remains to be one of the most common addictions worldwide. Even though most smokers wish to quit smoking, many of them fail. In this respect, genetic variants are thought to be remarkable factors in nicotine dependence and in treatment of smoking cessation. This is a paper investigating a single variant p-glycoprotein (P-gp) polymorphisms and its effect on Varenicline efficacy in the smoking cessation. 158 smokers and 52 non-smoker healthy volunteers were included. We determined the P-gp C3435T gene polymorphisms in all subjects. Face to face interviews with smokers were performed for smoking cessation and Varenicline was given for smoking cessation. Cessation success was evaluated in the 6th month and success...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Smoking Cessation/methods; Varenicline/analysis; Varenicline/adverse effects; Smoking/genetics; Genetic/drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100506

Drug analysis
Provedor de dados: 60
Autores: Singh,Anil Kumar.
Tipo: Info:eu-repo/semantics/review
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100026

Compatibility and stability of valsartan in a solid pharmaceutical formulation
Provedor de dados: 60
Autores: Júlio,Tamíris Amanda; Zâmara,Igor Fernando; Garcia,Jerusa Simone; Trevisan,Marcello Garcia.
Valsartan (VAL) is a highly selective blocker of the angiotensin II receptor that has been widely used in the treatment of hypertension. Active pharmaceutical ingredient compatibility with excipients (crospovidone, hypromellose, magnesium stearate, microcrystalline cellulose and titanium dioxide) is usually evaluated in solid pharmaceutical development. Compatibility and stability can be evaluated by liquid chromatography. Studies were performed using binary mixtures of 1:1 (w/w) VAL/excipient; samples were stored under accelerated stability test conditions (40 ºC at 75% relative humidity). The results indicate that VAL is incompatible with crospovidone and hypromellose, which reduced the VAL content and gave rise to new peaks in the chromatogram due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Valsartan/compatibility; Valsartan/stability; Hypertension/treatment; Differential Scanning Calorimetry/drug analysis; High performance liquid chromatography/quantitative analysis; Pharmaceutical formulations/quantitative analysis..
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400003

A critical analysis of the hepatotoxicity cases described in the literature related to Herbalife (r) products
Provedor de dados: 60
Autores: Zambrone,Flávio Ailton Duque; Corrêa,Cristiana Leslie; Amaral,Ligia Mesquita Sampaio do.
Abstract The aim of this study was to assess the hepatotoxicity cases described in the literature, attributed to the consumption of Herbalife(r) products, and to determine whether a causal relationship exists between the reported cases of liver injury and the use of these products. A literature search was performed on the PubMed, LILACS and PAHO databases. Seven publications reporting a total of 53 cases of hepatotoxicity linked to the use of Herbalife(r) products were retrieved. All of the studies lacked sufficient information to some degree, whether related to patients' history, concomitant use of medication and/or other compounds (including alcohol), observations on interrupted use (dechallenge), results found with markers, viral serology and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatotoxicity; Herbalife(r); Herbalife products(r)/consumption/hepatotoxicity; Dietary supplements; Causality assessment.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400785

Design and preparation of a novel colon-targeted tablet of hydrocortisone
Provedor de dados: 60
Autores: Ren,Yachao; Jiang,Lei; Yang,Shuman; Gao,Sainan; Yu,Hui; Hu,Jie; Hu,Dandan; Mao,Wenbin; Peng,Haisheng; Zhou,Yulong.
ABSTRACT The objective of this research was to design a new colon-targeted drug delivery system based on chitosan. The properties of the films were studied to obtain useful information about the possible applications of composite films. The composite films were used in a bilayer system to investigate their feasibility as coating materials. Tensile strength, swelling degree, solubility, biodegradation degree, Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Scanning Electron Microscope (SEM) investigations showed that the composite film was formed when chitosan and gelatin were reacted jointly. The results showed that a 6:4 blend ratio was the optimal chitosan/gelatin blend ratio. In vitro drug release results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitosan/evaluation; Chistosan/evaluation/gelatin complex film; Drugs/administration; Drugs/delivery system/Colon-targeting; Tablets/bilayer-coated.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100601

Pharmacists in dispensing drugs (PharmDisp): protocol for a clinical trial to test the effectiveness of distance education in training pharmacists for dispensing drugs
Provedor de dados: 60
Autores: Reis,Tiago Marques dos; Guidoni,Camilo Molino; Baldoni,André Oliveira; Giroto,Edmarlon; Obreli Neto,Paulo Roque; Ayres,Lorena Rocha; Pereira,Leonardo Régis Leira.
ABSTRACT Dispensing drug is a moment in which the pharmacist is able to analyze pharmacotherapy and contribute to its rational use. However, research has shown that some pharmacists lack adequate knowledge to perform this service. This study aims to describe a research protocol for a clinical trial to test the effectiveness of a distance learning program to train pharmacists in dispensing drugs. This is a protocol for an open diagnostic, non-randomized, single group clinical trial. A 12-week duration distance learning course was structured on the Moodle platform for training community pharmacists who are registered in the Regional Board of Pharmacy and work as employees or owners in Brazilian community pharmacies. The course curricula involves concepts and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacists/distant education; Pharmacists/dispensing of drugs; Pharmacists/continuing-education.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100605

Therapeutic approaches for tumor necrosis factor inhibition
Provedor de dados: 60
Autores: Barbosa,Maria Letícia de Castro; Fumian,Milla Machado; Miranda,Ana Luísa Palhares de; Barreiro,Eliezer J.; Lima,Lídia Moreira.
Tumor necrosis factor (TNF) consists of an inflammatory cytokine essential for homeostasis and organism defense. Despite its physiological relevance, both increased biosynthesis and release of TNF lead to the exacerbation of inflammatory and oxidative responses, which are related to the pathogenesis of a host of diseases of an inflammatory, autoimmune and/or infectious nature. In this context, effective therapeutic approaches for the modulation of TNF have been the focus of research efforts. Approximately one million individuals worldwide have been treated with biotechnological inhibitors of this cytokine, the so-called anti-TNF biopharmaceuticals. However, given the high risk of infection and the limitations related to cost and administration routes, new...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tumor necrosis factor; TACE; P38 MAPK; NF-κB; CAMP; Phosphodiesterase; Adenosine receptor.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300002

Validation of a spectrophotometric method to determine ciprofibrate content in tablets
Provedor de dados: 60
Autores: Nascimento,Guilherme Nobre Lima do; Rosa,Daniel Lerner da; Nishijo,Hisao; Aversi-Ferreira,Tales Alexandre.
Ciprofibrate is a drug indicated in cases of hypertriglyceridemia and mixed hyperlipidemia, but no monographs are available in official compendia for the analysis of this substance in tablets. The objective of this work was to develop and validate a spectrophotometric method for routine analysis of ciprofibrate in tablets. In this study, commercial and standard ciprofibrate were used, as well as placebo in absolute ethanol, analyzed by UV spectrophotometer. All tests followed the rules of Resolution RE-899, 2003. The results showed that the developed and validated method offers low cost, easy implementation, precision and accuracy, and may be included in the routine of quality control laboratories.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciprofibrate/analysis; Spectrophotometric method/medicines analysis; Medicines/quality control.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100004

Study of the correlation between blood cholinesterases activity, urinary dialkyl phosphates, and the frequency of micronucleated polychromatic erythrocytes in rats exposed to disulfoton
Provedor de dados: 60
Autores: Santos,Mariane Gonçalves; Vitor,Ricardo Vilela; Nakamura,Maurício Gustavo; Morelini,Luana de Souza; Ferreira,Rafaela Scalco; Paiva,Alexandre Giusti; Azevedo,Luciana; Marques,Vanessa Bergamin Boralli; Martins,Isarita; Figueiredo,Eduardo Costa.
Organophosphates (OPs) are widely used as pesticides, and its urinary metabolites as well as the blood cholinesterases (ChEs) activity have been reported as possible biomarkers for the assessment of this pesticide exposure. Moreover, the OPs can induce mutagenesis, and the bone marrow micronucleus test is an efficient way to assess this chromosomal damage. This paper reports a study carried out to verify the correlation among the disulfoton exposure, blood ChEs activity, urinary diethyl thiophosphate (DETP), and diethyl dithiophosphate (DEDTP), as well as micronucleated polychromatic erythrocytes (MNPCEs) frequency. Four groups of rats (n=12) were exposed to disulfoton at 0, 2.8, 4.7, and 6.6 mg kg-1 body weight. The blood ChEs activity, urinary DETP and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticides/exposure study; Organophosphate/exposure study; Disulfoton/exposure; Dialkyl phosphate/urinary excretion; Blood cholinesterase; Micronucleated polychromatic erythrocytes/frequency.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100016

Croton schiedeanus Schltd prevents experimental hypertension in rats induced by nitric oxide deficit
Provedor de dados: 60
Autores: Páez,María Teresa; Rodríguez,Diana Catalina; López,Daniel Fernando; Castañeda,Jorge Arturo; Buitrago,Diana Marcela; Cuca,Luis Enrique; Guerrero,Mario Francisco.
Croton schiedeanus Schltd (N.V.: "almizclillo") is a plant used in traditional medicine as an antihypertensive in Colombia. It contains flavonoid, diterpenoid and fenilbutanoid metabolites that have vasodilatation effects linked to the NO/cGMP pathway. This work aimed to assess the capacity of a 96% EtOH extract to prevent the hypertension induced by nitric oxide (NO) deficiency in rats. The NO synthase inhibitor L-NAME (10 mg/kg/d, i.p) was administered during five weeks to three groups of rats (6-7 animals): C. Schiedeanus (200 mg/kg/d, p.o), enalapril (reference, 10 mg/kg/d, p.o) and vehicle (control: olive oil 1 ml/kg/d, p.o). In addition, the blank group received only vehicle. The arterial blood pressure (BP) and heart rate (HR) were measured daily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Croton schiedeanus/pharmacognosy; Almizelillo/use; Almizelillo/hypertension prevention; Nitric oxide/deficit; Hypertension/prevention; Plant extracts/vasodilatation effects; Medicinal plants.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400027

Evaluation of cytochrome P-450 concentration in Saccharomyces cerevisiae strains
Provedor de dados: 60
Autores: Matuo,Míriam Cristina Sakuragui; Kikuchi,Irene Satiko; Pinto,Terezinha de Jesus Andreoli.
Saccharomyces cerevisiae has been widely used in mutagenicity tests due to the presence of a cytochrome P-450 system, capable of metabolizing promutagens to active mutagens. There are a large number of S. cerevisiae strains with varying abilities to produce cytochrome P-450. However, strain selection and ideal cultivation conditions are not well defined. We compared cytochrome P-450 levels in four different S. cerevisiae strains and evaluated the cultivation conditions necessary to obtain the highest levels. The amount of cytochrome P-450 produced by each strain varied, as did the incubation time needed to reach the maximum level. The highest cytochrome P-450 concentrations were found in media containing fermentable sugars. The NCYC 240 strain produced the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Cytochrome P-450/obtention; Cytochrome P-450/cultivation conditions; Ethanol; Carbon sources.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300011

Evaluation of medicines advertising in medical journals
Provedor de dados: 60
Autores: Souza,Daliana Maria Berenice de Oliveira; Lima,Suellen Cristiane Medeiros de; Batista,Almária Mariz; Carvalho,Maria Cleide Ribeiro Dantas de.
This work intended to analyze the advertising of medicines requiring medical prescription, divulged into three journals of the neurology and cardiology areas addressed to healthcare professionals. The analysis was based on current legislation, among other criteria, as well as specific literature. The presence of the following items was investigated: registration number, drug name, specific indications, contraindications; cautions and warnings; adverse reactions; possible side effects; posology; legibility of technical-scientific information and bibliographic references, phrases and/or expressions about the medication benefits, as compared to other drugs; safety warnings, healing promises and pictures of people smiling, and the quotations confirmation based...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicines/advertising; Medicines/prescription; Medicines/legislation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200016

Blood pressure levels and adherence to treatment of hypertensive patients, users of a school pharmacy
Provedor de dados: 60
Autores: Santos,Brigitte Rieckmann Martins dos; Teixeira,Juliana; Gonçalves,Débora; Gatti,Reynaldo Mascagni; Yavo,Boni; Aragão,Cicera Cristina Vidal.
Adherence to pharmacological treatment for hypertension is considered a key factor in guaranteeing successful therapy outcomes. Knowledge of the disease, its complications, as well as the need for changes in lifestyle, call for patient motivation and continuous interactive education. The evidence regarding the beneficial effects of changes in life style by hypertensive individuals in reducing the complications of the disease, as well as in its prevention are indisputable. However, the challenges posed by patient adherence to treatment prescribed by doctors remain. The aim of this study was to assess blood pressure levels together with degree of adherence to pharmacological treatment with Enalapril Maleate by means of the Morisky-Green Test, in hypertensive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adherence; Hypertension; Health Promotion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300004

Neutralizing potential of Rauvolfia serpentina root extract against Naja naja venom
Provedor de dados: 60
Autores: Sivaraman,Thulasi; Sreedevi,Nadooparambil Sasi; Meenachisundharam,Subramani; Vadivelan,Ramachandran.
Snake bites are a serious health hazard occurs throughout the world especially in tropical countries like India. Anti-Snake Venom Serum is the only remedy available to treat snake bite victims successfully till date. Infusion of ASV may lead to adverse reactions ranging from severe itching of the skin, hives to potentially serious allergic reactions. Considering all above difficulties research workers all over the world is constantly in search of a cheap and readily available easy formulate remedy for treating snake bite victims. In present study aqueous extract of Rauvolfia serpentina root was checked for the antidote properties against Naja naja venom by in vitro and in vivo methods. Various in vitro neutralization tests like Acetyl cholinesterase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake bite; Naja naja; Rauvolfia serpentina; Antivenom compounds.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100546

Antiplatelet activity of white and pink Nelumbo nucifera Gaertn flowers
Provedor de dados: 60
Autores: Durairaj,Brindha; Dorai,Arthi.
Nelumbo nucifera Gaertn (Nelumbonaceae) a plant used in Ayurvedic medicine (common name: lotus), is a perennial, large and rhizomatous aquatic herb most prevalent in South India. Preliminary phytochemical screening of both white and pink Nelumbo nucifera flowers revealed the presence of phytochemical constituents (flavonoids, alkaloids, phenols etc,). Hence, an attempt has been made to screen the effect of Nelumbo nucifera flowers (both types) on platelet aggregation. The antiplatelet activity of hydroethanolic extract of both types of flowers was studied using platelet-rich plasma in different concentrations (100-500µg/ml). Both white and pink Nelumbo nucifera flower extracts showed dose-dependent effective antiplatelet activity with maximum activity at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nelumbo nucifera Gaertn/phytochemical constituents; Nelumbo nucifera Gaertn/pharmacognosy; Nelumbo nucifera Gaertn/Antiplatelet activity; Ayurvedic medicine; Natural products; Aspirin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300023

Pharmaceutical films made from the waste material from the preparation of propolis extracts: development and characterization
Provedor de dados: 60
Autores: Toledo,Lucas de Alcântara Sica de; Bavato,Maíra Isabeli; Rosseto,Hélen Cássia; Cortesi,Rita; Bruschi,Marcos Luciano.
abstract This study investigated the development and characterized the physicochemical properties of films obtained from by-products (BP) from the preparation of propolis extracts. Films were produced in the presence and absence of a polymeric adjuvant (gelatin or ethylcellulose) and propylene glycol by a solvent casting method. Density, surface topography by scanning electron microscopy, mechanical properties (folding endurance, tensile strength and percentage elongation), water vapour permeability (WVP), moisture uptake capacity, thermogravimetry, differential scanning calorimetry and Fourier transform infrared spectroscopy (FTIR) were determined. The films were a transparent, light greenish-yellow colour, with a uniform surface, and were flexible and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis/byproducts; Propolis/use of waste material/films development; Films/propolis byproducts/physicochemical properties; Polymers; Ethylcellulose; Gelatin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400847

Evaluation of adherence to treatment by patients seen in a psychosocial care center in northeastern Brazil
Provedor de dados: 60
Autores: Souza,Thaís Teles; Silva,Wellington Barros; Onofre,Alexandre Sherlley Casimiro; Quintans,Jullyana de Souza Siqueira; Onofre,Fabiana Botelho de Miranda; Quintans-Júnior,Lucindo José.
In the treatment of mental disorders, nonadherence to medication, the main cause of psychiatric morbidity, is observed in about 50% of the cases and is responsible for numerous losses. This study evaluated adherence to drug treatment by patients seen in a Psychosocial Care Center (CAPS) in northeastern Brazil. Adherence to treatment was evaluated using the Haynes-Sackett and Morisky-Green-Levine tests. All patients registered in the CAPS were included in the study (n= 101). Only 11.88% of the patients adhered to drug treatment. The main reasons not to use medication were: oblivion (68.83%), feeling unwell after taking the medication (54.22%), not having money to buy the medication (43.83%), not finding the medication in the public health service (39.94%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Treatment adherence; Drug therapy/adherence to treatment; Mental health; Psychosocial Care Center.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400016

Potential nutritional and antioxidant activity of various solvent extracts from leaves and stem bark of Anisophyllea laurina R. Br ex Sabine used in folk medicine
Provedor de dados: 60
Autores: Onivogui,Gbago; Zhang,Xiaodan; Diaby,Mohamed; Maomy,Clotaire Gna; Song,Yuanda.
ABSTRACT Anisophyllea laurina is a plant that has been used in folk medicine to treat malaria, dysentery, diabetes, toothache and various skin diseases. Leaves extract had protein content of 9.68% and a high calcium content of 25084.317 mg/100 g while stem bark extract was found to contain greater amounts of calcium (8560.96 mg/100 g), potassium (7649.47 mg/100 g), magnesium (1462.49 mg/100 g) and iron (973.33 mg/100 g). Palmitic acid, linolenic acid, linoleic acid and oleic acid were the most abundant fatty acids in leaves and stem bark extracts. Furthermore, total phenolic (2382.39 mg GAE /100 g) and total flavonoid (385.79 mg QE/100 g) contents were abundant in stem bark while leaves extract was rich in total tannin content (3466.63 mg CE/100 g)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anisophyllea laurina/extract/ antioxidant activity; Anisophyllea laurina/extract/ total phenolic; Proximate composition; Flavonoid; Tannin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200631

The improvement of anti-HER2 scFv soluble expression in Escherichia coli
Provedor de dados: 60
Autores: Farshdari,Farzaneh; Ahmadzadeh,Maryam; Nematollahi,Leila; Mohit,Elham.
The relationship between the expression of HER2 and malignity of breast tumors has led to the generation of antibodies targeting HER2+ tumors. In addition, the expression of scFvs, as the smallest antigen-binding region of antibody containing two disulfide bonds in Escherichia coli often results in accumulating non-functional protein in the cytoplasm. A redox-modified strain of E. coli such as Origami (DE3) may facilitate the formation of proper disulfide bond in cytoplasm. The present study aimed to optimize the expression of anti-HER2 scFv in Origami and evaluate the influence of induction temperature, and host strain on the solubility of the protein. To this aim, chemicallysynthesized anti-HER2 scFv of Trastuzumab was cloned in pET-22b (+). The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; HER2; ScFv; Expression; Solubility.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100519

Chemical composition, antioxidant and biological activity of Ocotea bicolor Vattimo-Gil (LAURACEAE) essential oil
Provedor de dados: 60
Autores: Damasceno,Carolina Sette Barbosa; Oliveira,Letícia Freire de; Szabo,Ellis Marina; Souza,Ângela Maria; Dias,Josiane Fatima Gaspari; Miguel,Marilis Dalarmi; Miguel,Obdúlio Gomes.
ABSTRACT The essential oil composition of the Ocotea bicolor, native plant of Brazil, was studied for the first time. The essential oil of the leaf was obtained by hydrodistillation and analyzed by GC/MS. The analytical procedure revealed a predominance of sesquiterpenes, δ-cadinene (7.39%), β-sesquiphellandrene (6.67%), β-elemene (5.41%) and α-cadinol (5,23%). The essential oil was submitted to brine shrimp toxicity evaluation, antioxidant and antibacterial tests. The antioxidant activity by the formation of phosphomolybdenum complex method presented positive results. The minimum inhibitory concentration (MIC) values were higher than 1000 μg/mL for the microorganisms tested. Toxicity activity revealed LC50 results of 40.10 (μg/mL), being toxic to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocotea bicolor/Oil essential/chemical composition; Lauraceae; Antioxidant activity; Biological activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400615

Study of erythrocytes as a novel drug carrier for the delivery of artemether
Provedor de dados: 60
Autores: Riaz,Muhammad Ijaz; Sarwar,Hafiz Shoaib; Rehman,Mubashir; Gohar,Umar Farooq; Raza,Syed Atif; Siddique,Muhammad Irfan; Shahnaz,Gul; Sohail,Muhammad Farhan.
Resealed erythrocytes have been explored in various dimensions of drug delivery, owing to their high biocompatibility and inability to initiate immune response. The present research was designed to evaluate the drug delivery potential of erythrocytes by loading a hydrophobic anti-malarial drug, Artemether. Three different loading techniques were applied to achieve maximum optimized drug loading. A HPLC method was validated for drug quantification in erythrocytes. The relatively high loading was achieved using hypotonic treatment was 31.39% as compared to other two methods. These, drug loaded erythrocytes were characterized for membrane integrity via ESR showing higher ESR values for drug loaded cells as compared to normal cells. Moreover, microscopic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resealed Erythrocytes; Novel Drug Delivery System; Hydophobic drug/artemether.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100537

8-Propyl-6H-[1,3]dioxolo[4,5-g]chromen-6-one: A new coumarin with monoamine oxidase B inhibitory activity and possible anti-parkinsonian effects
Provedor de dados: 60
Autores: Olaya,María del Pilar; Vergel,Nadezdha Esperanza; López,Jose Luis; Viña,Dolores; Guerrero,Mario Francisco.
Parkinson’s disease is a common neurodegenerative disorder. In this study, the monoamine oxidase inhibitory activity and potential anti-parkinsonian effects of 8-propyl-6H-[1,3]dioxolo[4,5-g]chromen-6-one (FCS303), a new synthetic coumarin, were evaluated. To do this, we used the reserpine model of Parkinson’s disease, an assay of levodopa/carbidopa potentiation, the catalepsy model of haloperidol, and an in vitro assay against monoamine oxidase (MAO) activity. Additionally, lipid peroxidation and protein carbonyl group quantification was performed in mice brain homogenates previously treated with haloperidol. FCS303 inhibited monoamine oxidase B (MAO-B) with an IC50 of 5.46 ± 0.36 µM; however, there was no effect on monoamine oxidase A (MAO-A). The oral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson’s disease; Monoamine oxidase B; Coumarin; Mice; Reserpine; Levodopa; Carbidopa.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100508

Evaluation of antimicrobial, cytotoxic and chemopreventive activities of carvone and its derivatives
Provedor de dados: 60
Autores: Moro,Isabela Jacob; Gondo,Gabrielle Demmany Gualberto Alexandre; Pierri,Elaíse Gonçalves; Pietro,Rosemeire Cristina Linharis Rodrigues; Soares,Christiane Pienna; Sousa,Damião Pergentino de; Santos,André Gonzaga dos.
ABSTRACT Considering the reported activity of carvone in the literature, this study aimed to evaluate the antimicrobial, cytotoxic and chemopreventive activities of (+)- and (-)-carvone, (+)- and (-)- hydroxydihydrocarvone and α,β-epoxycarvone. (+)-Hydroxydihydrocarvone (HC+), (-)-hydroxydihydrocarvone (HC-) and α,β-epoxycarvone (EP) were obtained by synthesis using (+)-carvone (C+) or (-)-carvone (C-) as precursors. The antifungal activity (MIC and MFC) were evaluated against Candida parapsilosis, C. tropicalis, C. krusei and C. albicans and the antibacterial activity (MIC and MBC) against Escherichia coli and Staphylococcus aureus. The cytotoxicity assays were performed with human cancer cell lines HepG-2 and SiHa and the normal strain MRC-5 through...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carvone/antimicrobial activity; Carvone/antifungal activity; Carvone /cytotoxicity; Carvone/chemoprevention.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400602

In vitro investigations of Cynara scolymus L. extract on cell physiology of HepG2 liver cells
Provedor de dados: 60
Autores: Löhr,Gesine; Deters,Alexandra; Hensel,Andreas.
The objective of this study was the investigation of a potential influence of artichoke leaf extract (ALE) on the cell physiology and gene expression of phase I/II enzymes of human liver cells HepG2 and investigation on potential cell protective effects against ethanol-induced cell toxicity against HepG2 cells. Cell biological assays under in vitro conditions using HepG2 liver cells and investigation of mitochondrial activity (MTT test), proliferation assay (BrdU incorporation ELISA), LDH as toxicity marker, gene expression analysis by RT-PCR and enzyme activity of glutationtransferase. Artichocke extract, containing 27% caffeoylquinic acids and 7% flavonoids induced mitochondrial activity, proliferation and total protein content under in vitro conditions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cynara scolymus L. Asteraceae; Artichoke; Liver cells; Cell proliferation; Gene expression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200003

Lupine as a potential agent against diet-induced obesity through adenosine monophosphate-activated protein kinase pathway: crude oil versus nanoemulsion formulations
Provedor de dados: 60
Autores: Mohafez,Omar Mahmoud; Alhaider,Ibrahim Abdelrahman; Shehata,Tamer Mohamed; Mohamed,Maged Elsayed.
Obesity represents a major challenge to the pharmaceutical community due to the minimal availability of anti-obesity drugs and the drawbacks of current weight-loss agents. The study described herein presents lupine oil, in two pharmaceutical formulations, as a potential anti-obesity agent via its effect on different physiological, biochemical, and hormonal parameters. Rats were divided into two groups; one group was continued on a standard commercial rodent diet and served as the non-obese control. The other group was fed a high-fat diet for 7 weeks to prepare an obese rat model. Then, the obese rats were divided into groups to receive 100 mg/kg of the crude lupine oil or nanoemulsion for 10 or 20 days. Lupine oil showed a potent body weight-reducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adiponectin; Cholesterol; HDL; Leptin; Lupinus albus.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100561

Quality evaluation of simvastatin compounded capsules
Provedor de dados: 60
Autores: Marques-Marinho,Flávia Dias; Zanon,José Carlos da Costa; Sakurai,Emilia; Reis,Ilka Afonso; Lima,Angélica Alves; Vianna-Soares,Cristina Duarte.
Simvastatin is commercially available as tablets and compounded capsules in Brazil. Very few reports regarding these capsules' quality, and consequently their efficacy, are available. The pharmaceutical quality of 30 batches of 20 mg simvastatin capsules from the market was evaluated by weight determination, content uniformity, disintegration (Brazilian Pharmacopeia), assay and dissolution test (USP32 tablet monograph). A HPLC method was developed for assay, content uniformity and dissolution test, and specifications were also established. Out of the 30 batches evaluated, 29 showed capsule disintegration within 45 min and individual weight variation was within ±10% or ±7.5% relative to average weight, for &lt; or &amp;gt; 300 mg, respectively. Only 27...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compounded simvastatin capsules; Quality control; RP-HPLC.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300007

A simple and sensitive method for determination of Norfloxacin in pharmaceutical preparations
Provedor de dados: 60
Autores: Ye,Zhuo; Wang,Le; Wen,Jianguo.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Norfloxacin/determination; Graphene oxide; Nafion; Voltammetry/differential pulse; Pharmaceutical formulations/quantitative analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000200429

Design and evaluation of lornoxicam bilayered tablets for biphasic release
Provedor de dados: 60
Autores: Sunil,Songa Ambedkar; Srikanth,Meka Venkata; Rao,Nali Sreenivasa; Balaji,Sakamuri; Murthy,Kolapalli Venkata Ramana.
The objective of the present investigation was to develop bilayered tablets of lornoxicam to achieve biphasic release pattern. A bilayered tablet, consisting of an immediate and controlled release layer, was prepared by direct compression technique. The controlled release effect was achieved by using various hydrophilic natural, semi synthetic and synthetic controlled release polymers such as xanthan gum, hydroxypropyl methylcellulose (HPMC) and polyethylene oxide (PEO) to modulate the release of the drug. The in vitro drug release profiles showed the biphasic release behavior in which the immediate release (IR) layer containing the lornoxicam was released within 15 minutes, whereas the controlled release (CR) layer controlled the drug release for up to 24...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lornoxicam/bilayered tablet; Lornoxicam/development; Bilayered tablet/biphasic release; Hydrophilic polymers/use; Hydrophilic polymers/drug release; Drugs/controlled release.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400004

Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form
Provedor de dados: 60
Autores: Panda,Sagar Suman; Kumar,Bera Venkata Varaha Ravi; Mohanta,Ganeswar.
A novel, simple, accurate and precise RP-HPLC method for simultaneous determination of levosalbutamol sulfate and theophylline has been developed and validated. Separation was achieved on a Phenomenex; C18 column (250 mm × 4.6 mm i.d., 5 µm) using methanol: 10 mM TBAHS(tetrabutyl ammonium hydrogen sulfate) (50:50, v/v) as mobile phase at flow rate of 1.0 mL.min-1. The UV detection wavelength was 274 nm. The linearity is obeyed over a concentration range of 0.5-150 µg.mL-1 with correlation coefficient of 0.999 for both the drugs. The proposed method was validated by determining accuracy, precision, stability and system suitability parameters. The method was found to be robust. Specificity of the method was determined by subjecting the drugs to various...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Levosalbutamol/determination; Theophylline/determination; Stability-indicating; RP-High performance liquid chromatography/qualitative analysis Syrup; RP-High performance liquid chromatography/qualitative analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300009

Estimated glomerular filtration rate and drug dose adjustment in patients in an intensive care unit in Brazil
Provedor de dados: 60
Autores: Camargo,Marianne Silveira; Oliveira,Márcio Galvão; Mistro,Sostenes; Rocha,Izabel Cristina Pereira; Santos,Camila Neto; Castro,Caroline Tianeze de; Passos,Luiz Carlos Santana.
Objectives. This study sought to compare the estimated glomerular filtration rate and the indication of dose adjustment of antimicrobials when using Cockcroft-Gault or Modification of Diet in Renal Disease. Methods. A cross-sectional study was performed with patients admitted to the intensive care unit of a Brazilian general hospital. The glomerular filtration rate was calculated for patients on all days using the Cockcroft-Gault and Modification of Diet in Renal Disease equations. The difference in estimated glomerular filtration and the dose adjustment indication of antimicrobials were assessed. Results. A total of 631 patients were included in this study. The median estimated glomerular filtration was significantly higher when estimated using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intensive Care Units; Glomerular Filtration Rate; Anti-infective Agents; Renal Insufficiency.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100595

Exploring the shared genes of hypertension, diabetes and hyperlipidemia based on microarray
Provedor de dados: 60
Autores: Dong,Wenzhu; Chen,Hangping; Wang,Lu; Cao,Xiaoqian; Bu,Xiawei; Peng,Yan; Dong,Aiqing; Ying,Mengjiang; Chen,Xu; Zhang,Xin; Yao,Li.
Given their relationship with metabolic syndrome and systematic inflammatory diseases, the pathogenesis of hypertension, hyperglycemia, and hyperlipidemia is closely related. To explore the common genes among these three conditions, spontaneous hypertensive rats (SHR), spontaneous diabetic Goto-Kakizaki rats (GK) and hyperlipidemia rats (HMR) were reared for experiments. Gene array was used to identify the genes of SHR, GK and HMR compared with normal Wistar rats using TBtools software. First, real-time PCR was applied to verify these genes, and Cytoscape software was used to construct networks based on the National Center for Biotechnology Information (NCBI) database. Second, Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis was performed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Diabetes; Hyperlipidemia; Genes; Microarray; Neoplasm.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100566

In vitro intestinal permeability studies, pharmacokinetics and tissue distribution of 6-methylcoumarin after oral and intraperitoneal administration in Wistar rats
Provedor de dados: 60
Autores: Cárdenas,Paola Andrea; Kratz,Jadel Müller; Hernández,Aura; Costa,Geison Modesti; Ospina,Luis Fernando; Baena,Yolima; Simões,Cláudia Maria Oliveira; Jimenez-Kairuz,Álvaro; Aragon,Marcela.
ABSTRACT 6-Methylcoumarin (6MC) is a semisynthetic coumarin with important in vitro and in vivo anti-inflammatory activity. In order to continue the pre-clinical characterization of this molecule, in vitro intestinal permeability, plasma profile and tissue distribution after oral administration in rats were studied. The permeability of 6MC was evaluated by the Caco-2 cellular model in both the apical-basal (A-B) and basal-apical (B-A) directions. The pharmacokinetics and biodistribution were evaluated in rats after oral and intraperitoneal administration at doses of 200 mg/kg. Transport experiments with Caco-2 cells showed that 6MC presented high permeability at all concentrations evaluated. This finding suggested that 6MC could be transported across the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 6-Methylcoumarin/Pharmacokinetics/rats; 6-Methylcoumarin/distribution; Intestinal permeability/study; Intestinal permeability/study/In vitro.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100621