Consulta: subjectFacets:"Escherichia coli"
Registros recuperados: 237
Data/hora: 01/06/2024 08:05:37

Atmósferas modificadas en modalidad de microperforado, enterobacterias y residuos de plaguicidas en nopal verdura (Opuntia ficus indica L.).
Provedor de dados: 32
Autores: Meraz Maldonado, Nora.
El nopal verdura es una hortaliza que aporta cantidades importantes de nutrimentos a la dieta en México. Sin embargo, cuando éste es usado como producto mínimamente procesado, la vida de anaquel se limita a los cambios en coloración de la piel y heridas pero, también debe ser considerada, dentro de la calidad, la inocuidad relacionada a la contaminación química y microbiológica. El presente estudio aborda éstos aspectos en seis capítulos que tienen por objetivos: 1) Determinar la predilección del consumidor en la adquisición de nopal verdura por tamaño, color, presentación, acidez y fibra. 2) Evaluar algunos cambios en la calidad por efecto del tamaño en nopal verdura entero desespinado. 3) Determinar el efecto de las atmósferas modificadas en la calidad...
Palavras-chave: Opuntia ficus-indica; Atmósferas modificadas; Salmonella; Escherichia coli; Malatión; Clorpirifos etílico; Entomología y Acarología; Doctorado; Modified atmospheres; Malathion; Chlorpyrifos ethyl.
Ano: 2012
URL: http://hdl.handle.net/10521/1819

Calidad sanitaria en la producción de nopal verdura (Opuntia sp.) en Otumba, México.
Provedor de dados: 32
Autores: Martínez Martínez, Talina Olivia.
La calidad sanitaria en los productos hortofrutícolas debe considerarse una prioridad en la producción de alimentos. Actualmente en México, existe una gran preocupación por las asociaciones de productores y gobierno federal para adoptar sistemas de producción que reduzcan riesgos de contaminación (SRRC). Para la implementación de estos sistemas, es necesario cumplir con una serie de requisitos, entre ellos se encuentra la validación de procesos. Un método inmediato para efectuar este requerimiento, es la evaluación de la calidad sanitaria desde el punto de vista microbiológico y toxicológico, lo que hace necesario determinar un plan de muestreo y las técnicas apropiadas para la detección y cuantificación de patógenos indicadores. Con este propósito, en...
Palavras-chave: Salmonella; Escherichia coli; Pesticides; Phenolic compounds; Survival; Sample size; Entomología y Acarología; Doctorado.
Ano: 2011
URL: http://hdl.handle.net/10521/590

Efficient expression and characterization of a cold-active endo-1, 4-&amp;#946;-glucanase from Citrobacter farmeri by co-expression of Myxococcus xanthus protein S
Provedor de dados: 69
Autores: Bai,Xi; Yuan,Xianjun; Wen,Aiyou; Li,Junfeng; Bai,Yunfeng; Shao,Tao.
Background: Cold-active endo-1, 4-&amp;#946;-glucanase (EglC) can decrease energy costs and prevent product denaturation in biotechnological processes. However, the nature EglC from C. farmeri A1 showed very low activity (800 U/L). In an attempt to increase its expression level, C. farmeri EglC was expressed in Escherichia coli as an N-terminal fusion to protein S (ProS) from Myxococcus xanthus. Results: A novel expression vector, pET(ProS-EglC), was successfully constructed for the expression of C. farmeri EglC in E. coli. SDS-PAGE showed that the recombinant protein (ProS-EglC) was approximately 60 kDa. The activity of ProS-EglC was 12,400 U/L, which was considerably higher than that of the nature EglC (800 U/L). ProS-EglC was active at pH 6.5-pH 8.0,...
Tipo: Journal article
Palavras-chave: Cellulose degradation; Cellulose; Cold-active enzyme; Endoglucanases; Enzymatic properties; Escherichia coli; Expression; Novel expression vector; N-terminal fusion; Protein S-tag; Recombinant protein.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600012

Fermentation optimization of maltose-binding protein fused to neutrophil-activating protein from Escherichia coli TB1
Provedor de dados: 69
Autores: Lu,Jike; Song,Qi; Ji,Zhenyu; Liu,Xin; Wang,Ting; Kang,Qiaozhen.
Background The fermentation conditions of recombinant maltose-binding protein fused to neutrophil-activating protein (rMBP-NAP) of Helicobacter pylori were optimized from Escherichia coli TB1 with varying medium, inoculum age and size, time, inducer, pH and temperature in batch fermentation. Results It was revealed that the optimal conditions for the production of rMBP-NAP in shake flask were as follows: M9 medium (with 3% yeast extract powder added), inoculum age of 19 h, inoculum size of 6%, initial pH of 6.6, temperature of 37°C, and 0.7 mmoL/L IPTG inducted 21 h in a 50 mL/250 mL shake flask. The recombinant protein yield was increased from 59 to 592 mg/L after optimization. Fermentation process conducted in a 10 L fermenter with similar conditions...
Tipo: Journal article
Palavras-chave: Escherichia coli; Fermentation; Optimization; Recombinant protein.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400004

Engineering Escherichia coli for autoinducible production of n-butanol
Provedor de dados: 69
Autores: Wang,Qinglong; ding,Yi; Liu,Li; Shi,Jiping; Sun,Junsong; Xue,Yongchang.
Background Escherichia coli does not produce n-butanol naturally, but can be butanologenic when related enzymes were expressed using inducible elements on plasmids. In this study we attempted to confer E. coli strain capability of automatic excretion of the chemical by employing a native anaerobic promoter. Also, a novel DNA kit was designed for PCR preparation of linear DNA fragments to perform strain modification. The kit is primarily composed of two mother vectors, co-transformation of linear DNAs into E. coli can simultaneously introduce two butanol synthetic operons into the chromosome and create two in-frame gene deletions at targeted native loci. Results E. coli strain Bw2V carries plasmid pCNA-PHC and pENA-TA, both utilizes native anaerobic...
Tipo: Journal article
Palavras-chave: Anaerobic promoter; Escherichia coli; Metabolic engineering; N-Butanol; Recombination.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200013

Expression and purification of soluble single-chain Fv against human fibroblast growth factor receptor 3 fused with Sumo tag in Escherichia coli
Provedor de dados: 69
Autores: Liu,Zixuan; Zhang,Jizhou; Fan,Hongqiong; Yin,Ruofeng; Zheng,Zhong; Xu,Qian; Liu,Qing; He,Haiting; Peng,Xiaofan; Wang,XinXin; Li,Xiaokun; Xiao,Yechen.
Background Overexpression or mutated activation of Fibroblast growth factor receptor 3 (FGFR3) is involved in the pathogenesis of many tumors. More and more studies focus on the potential usage of therapeutic antibodies against FGFR3. Results In this study, a novel single-chain Fv (ScFv) against FGFR3 was prepared and characterized. To achieve the soluble expression, ScFv was fused with Sumo (Small ubiquitin-related modifier) by polymerase chain reaction (PCR), and cloned into pET-20b. The recombinant bacteria were induced by 0.5 mM Isopropyl-ß-d-thiogalactopyranoside (IPTG) for 16 h at 20°C, and the supernatant liquid of Sumo-ScFv was harvested and purified by Ni-NTA chromatography. After being cleaved by the Sumo protease, the recombinant ScFv was...
Tipo: Journal article
Palavras-chave: Escherichia coli; Fibroblast growth factor receptor 3; Single-chain Fv antibody; Soluble expression; Sumo tag.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400008

Design and expression of a retro doublet of cecropin with enhanced activity
Provedor de dados: 69
Autores: Díaz,Mauricio; Arenas,Gloria; Marshall,Sergio H.
Novel doublet molecules of cecropin A from Drosophila melanogaster were designed and constructed combining the regular (CECdir) with the inverted (CECret) coding sequence of the standard CEC A1 gene resulting in the following configurations: CECdir-CECret and CECret-CECdir. These two recombinant molecules were generated using a three-primer driven PCR reaction yielding composite single functional aminoacidic molecules with the coding sequences of CECdir linked in frame with the coding sequence of CECret and vice versa. In order to obtain these constructions, a retropeptide DNA-coding sequence was chemically synthesized to match the expected polarity of the newly generated CECret sequence. Both doublet antimicrobial peptides (drAMPs) were cloned in the T7...
Tipo: Journal article
Palavras-chave: Antimicrobial peptides; Escherichia coli; Expression.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200006

Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution
Provedor de dados: 69
Autores: Alves,Carlucio R; Pimenta,Maria Gardenny R; Vieira,Regine H.S.F; Furtado,Roselayne F; Guedes,Maria Izabel F; Silva,Rui C.B; Assis,Odilio B.G.
The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy and atomic force microscopy techniques. Bactericide activity of the enzyme was evaluated by spectrophotometric analysis. It was verified that the enzymatic film was strongly coupled with the vitreous surface. The topographic analysis demonstrated that the deposited film was uniform and homogeneous. It was observed bactericide activity of film deposited on vitreous surface with 0.3 mm in fresh and saline solutions. This fact was not verified to vitreous fragments...
Tipo: Journal article
Palavras-chave: Escherichia coli; Lysozyme; Self-assembly.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100015

Metabolic engineering: Use of system-level approaches and application to fuel production in Escherichia coli
Provedor de dados: 69
Autores: Gonzalez,Ramon.
Metabolic engineering was formally defined more than two decades ago (Bailey, 1991) and it is now an established discipline. Metabolic engineering is generally defined as the directed improvement of product formation or cellular properties through the modification of specific biochemical reactions or the introduction of new ones with the use of recombinant DNA technology (Bailey, 1991; Stephanopoulos et al. 1998). Therefore, the analysis and engineering/synthesis of metabolic pathways is of central importance to metabolic engineering. The analytical part uses a number of experimental and modeling techniques for the systematic study of cellular responses (in terms of RNA, protein and metabolite levels, metabolic fluxes, etc.) to genetic and environmental...
Tipo: Journal article
Palavras-chave: Biofuels; Escherichia coli; Metabolic engineering; Systems biology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300017

Production of antibody fragment (Fab) throughout Escherichia coli fed-batch fermentation process: Changes in titre, location and form of product
Provedor de dados: 69
Autores: Jalalirad,Reza.
Background: Recombinant proteins, including antibodies and antibody fragments, often contain disulfide bond bridges that are necessary for their folding, stability and function. Production of disulfide-bond-containing proteins in the periplasm of Escherichia coli has been very useful, due to unique characteristics of the periplasm, for obtaining fully active and correctly folded products and for alleviating downstream processing. Results: In this study, fed-batch cultivation of Escherichia coli (E. coli) for production of Fab D1.3, which is an anti-hen egg white lysozyme (HEWL) antibody fragment was carried out at 37ºC, and the bacterial cells were induced by adding 0.1 mM IPTG to the culture medium. Fermentor was sampled over the course of fermentation;...
Tipo: Journal article
Palavras-chave: Antibody fragment; Cell disruption; Escherichia coli; Fed-batch fermentation; Periplasmic expression; Recombinant protein.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300009

Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion
Provedor de dados: 74
Autores: Wang,Zhongshan; Xiang,Quanju; Wang,Guangjun; Wang,Haiyan; Zhang,Yizheng.
The cloning, expression and purification of the glutathione (sulfur) import system ATP-binding protein (gsiA) was carried out. The coding sequence of Escherichia coli gsiA, which encodes the ATP-binding protein of a glutathione importer, was amplified by PCR, and then inserted into a prokaryotic expression vector pWaldo-GFPe harboring green fluorescent protein (GFP) reporter gene. The resulting recombinant plasmid pWaldo-GFP-GsiA was transformed into various E. coli strains, and expression conditions were optimized. The effect of five E. coli expression strains on the production of the recombinant gsiA protein was evaluated. E. coli BL21 (DE3) was found to be the most productive strain for GsiA-GFP fusion-protein expression, most of which was insoluble...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Glutathione transporter; GsiA; Gene expression; Green fluorescent protein.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400019

Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli
Provedor de dados: 74
Autores: Chuan-Xiao,Xie; An,Xu; Li-Jun,Wu; Jian-Min,Yao; Jian-Bo,Yang; Zeng-Liang,Yu.
To identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif r) mutant screening and sequencing of the defined region of the rpoB gene in E. coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or 60Co-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;TA transitions were significantly increased from 32 to 46, AT &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Base substitution; Low-energy nitrogen ion beam implantation; 60Co-gamma rays; Escherichia coli.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200025

Etude sanitaire de la zone 50.04 « Utah Beach – Quinéville » - groupes 2 et 3 (coquillages bivalves fouisseurs et non fouisseurs)
Provedor de dados: 5
Autores: Rocq, Sophie.
Suite à la demande des pêcheurs professionnels de pouvoir exploiter les coques (coquillages bivalves fouisseurs) dans la zone 50.04 « Utah Beach – Quinéville », la Direction Départementale des Territoires et de la Mer a demandé la réalisation d’une étude sanitaire en vue du classement de la zone de production pour ce groupe de coquillages. Une étude de dossier et une inspection du littoral ont été réalisées afin de recenser les principales sources de contamination microbiologique et d’évaluer leurs impacts sur la zone étudiée. Ce travail a suggéré que le risque de contamination microbiologique était dispersé tout au long du littoral étudié. Trois points de prélèvement de coques (répartis dans le secteur où se trouve le gisement) ont été échantillonnés deux...
Tipo: Text
Palavras-chave: Escherichia coli; Étude sanitaire; Manche; Classement sanitaire; Contamination bactériologique des coquillages; Coques; Moules; Fouisseurs; Non fouisseurs; Utah Beach.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00664/77651/79685.pdf

Qualité du Milieu Marin Littoral - Bulletin de la surveillance - Edition 2009 - Départements : Charente- Maritime &amp; Vendée (Sud)
Provedor de dados: 5
Autores: Ifremer.
Le Laboratoire Environnement Ressources des Pertuis Charentais opère, sur le littoral des départements de Charente-Maritime et de Vendée (partie sud), les réseaux de surveillance nationaux1 de l'Ifremer dont une description succincte est présentée ci-dessous. Les résultats figurant dans ce bulletin sont obtenus à partir de données validées extraites de la base Ifremer Quadrige (base des données de la surveillance de l'environnement marin littoral), données recueillies jusqu’en 2008, REMI Réseau de contrôle microbiologique : REPHY Réseau de surveillance du phytoplancton et des phycotoxines, ROCCH Réseau d’observation de la contamination chimique, REMORA Réseau mollusques des ressources aquacoles, REMOULA Réseau régional de suivi de la croissance des moules
Tipo: Text
Palavras-chave: Surveillance pollution; Milieu marin; Réseau observation; Zone littorale; Phytoplancton; Metal lourd; Toxicité; Escherichia coli; Alexandrium; Dinophysis; Charente Maritime; Vendée Sud.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00041/15213/12540.pdf

Validity of Escherichia coli, enterovirus, and F-specific RNA bacteriophages as indicators of viral shellfish contamination
Provedor de dados: 5
Autores: Miossec, Laurence; Le Guyader, Soizick; Pelletier, Dominique; Haugarreau, Larissa; Caprais, Marie-paule; Pommepuy, Monique.
The sanitary classification of harvesting areas for bivalve mollusks in France is based on the level of Escherichia Coli contamination detected in shellfish meat, as defined in EC Directive 91/492 EEC. However, outbreaks of gastroenteritis or hepatitis after consumption of shellfish meeting current bacteriological standards suggest that E. coli is a poor indicator of viral contamination. The purpose of this study was to assess the adequacy of enterovirus and F-specific RNA bacteriophages as new indicators of human enteric viruses. Shellfish were sampled over a 37-mo period to characterize microbial contamination in two coastal areas subjected to different sewage contamination inputs. Contamination by E. coli, F-specific RNA bacteriophages (F+ RNA) and...
Tipo: Text
Palavras-chave: Indicator; Escherichia coli; F specific RNA bacteriophages; Enteric viruses; Viral contamination; Shellfish.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/00000/10006/7794.pdf

Qualité du Milieu Marin Littoral - Bulletin de la surveillance - Edition 2010 - Départements : Charente- Maritime &amp; Vendée (Sud)
Provedor de dados: 5
Autores: Ifremer.
Le Laboratoire Environnement Ressources des Pertuis Charentais opère, sur le littoral des départements de Charente-Maritime et de Vendée (partie sud), les réseaux de surveillance nationaux de l'Ifremer dont une description succincte est présentée ci-dessous, ainsi que des réseaux régionaux. Les résultats figurant dans ce bulletin sont obtenus à partir de données validées extraites de la base Ifremer Quadrige (base des données de la surveillance de l'environnement marin littoral), données recueillies jusqu’en 2009 : REMI Réseau de contrôle microbiologique, REPHY Réseau de surveillance du phytoplancton et des phycotoxines, ROCCH Réseau d’observation de la contamination chimique, REMORA Réseau mollusques des ressources aquacoles, REMOULA Réseau régional de...
Tipo: Text
Palavras-chave: Surveillance pollution; Milieu marin; Réseau observation; Zone littorale; Phytoplancton; Metal lourd; Toxicité; Escherichia coli; Alexandrium; Dinophysis; Charente Maritime; Vendée Sud.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00041/15216/12541.pdf

Etude sanitaire de la zone 14.031 « De l'estuaire de la Dives à Merville-Franceville » - groupe 2 (coquillages fouisseurs)
Provedor de dados: 5
Autores: Rocq, Sophie.
La Direction Départementale des Territoires et de la Mer (DDTM) du Calvados a demandé la réalisation d’une étude sanitaire pour la zone 14.031 « De l'estuaire de la Dives à Merville-Franceville », qui a été classée pour le groupe 2 (coquillages fouisseurs) mais sans étude sanitaire préalable. Une étude de dossier a été réalisée afin de recenser les principales sources de contamination microbiologique et d’évaluer leurs impacts sur la zone étudiée. Trois points de prélèvement de coques ont été échantillonnés deux fois par mois entre février 2019 et janvier 2020, en vue de l’analyse de leur concentration en Escherichia coli (indicateur de contamination fécale). Pour deux de ces points, la ressource aléatoire n’a pas permis d’avoir suffisamment de résultats...
Tipo: Text
Palavras-chave: Escherichia coli; Étude sanitaire; Calvados; Classement sanitaire; Contamination bactériologique des coquillages; Coques; Fouisseurs; Dives; Cabourg; Merville- Franceville-Plage.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00629/74114/73550.pdf

Dénombrement des Escherichia coli dans des sédiments de la baie de l'Aiguillon
Provedor de dados: 5
Autores: Derrien, Annick.
Le rôle des sédiments dans la contamination du milieu marin en baie de l'Aiguillon est une des problématiques du laboratoire. Pendant quinze mois des dénombrements d'Escherichia coli dans des sédiments de la baie ont été effectués. Deux méthodes de numération ont été comparées: la technique NPP en tubes et la technique NPP en microplaque. En période printemps-été, la méthode NPP miniaturisée a conduit à des résultats sensiblement supérieurs à ceux obtenus par la méthode NPP en tubes. Cette différence pourrait s'expliquer par une fluorescence parasite du sédiment pendant cette période (éventuellement par du plancton; hypothèse non confirmée). Les concentrations en E.coli des sédiments sont variables au cours de l'année avec un niveau de contamination faible...
Tipo: Text
Palavras-chave: Sédiments; Escherichia coli; Dénombrement.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00107/21811/19386.pdf

Evaluation de la qualité des zones de production conchylicole. Département de Loire-Atlantique. Edition 2021
Provedor de dados: 5
Autores: Schmitt, Anne; Piquet, Jean-come.
Après un rappel des objectifs, du fonctionnement et la méthode d’interprétation des résultats, ce rapport décrit les résultats obtenus en 2020 pour le réseau de surveillance de la contamination microbiologique REMI et du réseau d’observation de la contamination chimique ROCCH du département de Loire-Atlantique. Il présente l’estimation de la qualité microbiologique et chimique des zones de production de coquillages classées. En 2020, il y a eu 32 alertes déclenchées : 24 de niveau 0, 7 de niveau 1 et 1 de niveau 2. Sur 29 zones classées, deux zones présentes des discordances de classement et quatre zones n’ont pas suffisamment de données pour être classées. Sur les deux zones dont le classement sanitaire ne concorde pas avec l’estimation de la qualité, les...
Tipo: Text
Palavras-chave: Ifremer; REMI; Escherichia coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Estimation de la qualité sanitaire des zones de production; Département de Loire-Atlantique.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00691/80289/83402.pdf

Etude sanitaire microbiologique. Ria de la rivière de Morlaix
Provedor de dados: 5
Autores: Monfort, Patrick; Lebrun, Luc.
La rivière de Morlaix est traditionnellement et exclusivement tournée vers la production d’huîtres creuses depuis des décennies. Dans ce contexte d’activité conchylicole, elle fait l’objet d’un classement sanitaire pour les coquillages du groupe 3 pris par arrêté préfectoral n° 2012 2361-0003 en date du 26/12/2012. Le Comité Régional de la Conchyliculture (CRC) et le Comité Régional des Pêches Maritimes et des Elevages Marins (CRPMEM) ont conjointement sollicité la Direction Départementale des Territoires et de la Mer (DDTM) pour entreprendre une étude sanitaire sur les coquillages du groupe 2 (coques et palourdes). Cette demande s’inscrit dans un double objectif de diversification de la production conchylicole dans un contexte difficile de mortalités...
Tipo: Text
Palavras-chave: Rivière de Morlaix; Contamination fécale; Escherichia coli; Plomb; Cadmium; Mercure; Dioxines; Polychlorobiphényles; Benzo(a)pyrène; Zone conchylicole; Palourde.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00276/38735/37252.pdf

Etude de la biomasse bactérienne dans le bassin de Marennes-Oléron
Provedor de dados: 5
Autores: Heral, Maurice; Prou, Jean.
A seasonal study of total microflore shows that heterotrophic germs are submitted to a distinctive evolution in autumn and winter suspension and degradation of detritic material; in spring an summer bacterial growth due to degradation of phytoplanction. In summer enterocoques give evidence of positive summer pollution. Survival on the mud of Escherichia coli and overall of enterocoques indicates that the substration is particularly suitable to germ proliferation;
Tipo: Text
Palavras-chave: Marennes Oléron; Escherichia coli; Bactériologie; Biomasse.
Ano: 1980
URL: http://archimer.ifremer.fr/doc/1980/acte-3005.pdf

Les études sanitaires et la pêche à pied professionnelle : l'exemple de Bonne Anse en Charente-Maritime
Provedor de dados: 5
Autores: Piquet, Jean-come.
Tout coquillage mis en vente est issu d’une zone de production (gisement naturel ou concession) classée et surveillée au niveau sanitaire. Quatre classements sont définis de A à D par ordre décroissant de salubrité, du classement A (bonne qualité) autorisant la commercialisation directe des coquillages, au classement D (très mauvaise qualité). Pour définir ce classement, une étude sanitaire est préalablement conduite par l’Ifremer sur demande de l’Administration. L’étude sanitaire réalisée à Bonne Anse en Charente-Maritime, a abouti en 2009 à un arrêté préfectoral classant en B le gisement de palourdes. Aujourd’hui, la qualité sanitaire de la zone est surveillée régulièrement par l’Ifremer, et environ 25 pêcheurs à pied professionnels exploitent le gisement.
Tipo: Text
Palavras-chave: Etude sanitaire; Pêche à pied professionnelle; Charente-Maritime; Bonne Anse; Escherichia coli; Cadmium.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00048/15965/13405.pdf

Caractérisation hydrologique et microbiologique du bassin versant de Sainte Anne (Plouzané)
Provedor de dados: 5
Autores: Belloir, Jerome.
The coastline has many activities related to water quality such as bathing activities. Coastal catchments are known to be source of microbial input to the marine environment, their flows and runoff bringing important quantities of sediment, bacteria and other particles. Thus, a network of monitoring and modeling may be necessary to prevent economic losses such as a closure of beaches due to contamination. Hence, within the framework of project GIRAC, a modeling of river and E. coli flow in the Sainte-Anne catchment was developed. To implement the model AVSWAT on the catchment, a data collection (stream gauging, E. coli analyses) and work on GIS was necessary. The aim of this study is the hydrological and microbiological characterization of the catchment....
Tipo: Text
Palavras-chave: Bassin versant; Débits; Escherichia coli; SIG; Sainte-Anne; Catchment; River flow; Escherichia coli; GIS; Sainte-Anne.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00044/15571/12969.pdf

Etude sanitaire microbiologique. Ria de la rivière du Faou
Provedor de dados: 5
Autores: Monfort, Patrick; Lebrun, Luc.
L'activité conchylicole sur la rivière du Faou se résume pour l'heure à l'élevage d'huîtres creuses pour lequel d'estuaire est classé en zone de salubrité B. Suite à la sollicitation du Comité Régional des Pêches Maritimes et des Elevages Marins (CRPMEM) de Bretagne pour y entreprendre une étude sanitaire sur les coquillages du groupe 2 (palourdes), l'administration départementale a donné un avis favorable et mission l'Institut Français de Recherche pour l'Exploitation de la MER (Ifremer) pour mener à bien cette étude. Les résultats obtenus ont mis en évidence une qualité chimique satisfaisante des palourdes et à l'opposé, une qualité bactériologique médiocre, induisant une estimation de la qualité en zone de salubrité C pour ces bivalves du groupe 2. En...
Tipo: Text
Palavras-chave: Rivière du Faou; Contamination fécale; Escherichia coli; Plomb; Cadmium; Mercure; Dioxines; Polychlorobiphényles; Benzo(a)pyrène; Zone conchyli cole; Palourdes.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00249/36053/34603.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par Escherichia coli. Sous-région marine Golfe de Gascogne. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Amouroux, Isabelle.
Tipo: Text
Palavras-chave: Golfe de Gascogne; Coquillages; Escherichia coli; Contamination; Réglementation; Qualité microbiologique; Evolution.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00329/44060/43631.pdf

Evaluation de la qualité des zones de production conchylicole. Département de Loire-Atlantique. Edition 2019
Provedor de dados: 5
Autores: Piquet, Jean-come; Schmitt, Anne; Rocq, Sophie; Cochennec-laureau, Nathalie.
Après un rappel des objectifs, du fonctionnement et de la méthode d’interprétation des résultats, ce rapport décrit les résultats obtenus en 2018 pour le réseau de surveillance de la contamination microbiologique REMI et du réseau d’observation de la contamination chimique ROCCH du département de Loire–Atlantique. Il présente l’estimation de la qualité microbiologique et chimique des zones de production de coquillages classées.
Tipo: Text
Palavras-chave: Ifremer; REMI; Escherichia coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Estimation de la qualité sanitaire des zones de production; Département de Loire-Atlantique.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00495/60625/64117.pdf

Comparaison de methodes directes (microscopiques) et indirectes (par mise en culture) dans l'evaluation d'une pollution bacterienne d'origine fecale. Etude preliminaire
Provedor de dados: 5
Autores: Munro, P; Bianchi, M.
Cells of two serotypes of Escherichia coli (O26B6 and 0111B4) were counted using epifluorescence and immunofluorescence microscopy (nonavalent serum) and plate count on specific medium. Values from indirect counts were 10 fold smaller than those from both microscopic counts. In a sample of sea water from an area receiving a fecal pollution, the highest number of bacteria was obtained by epifluorescence microscopy. In that case, the number of cells counted by immunofluorescence microscopy was 10 fold smaller than the number of bacteria able to grow on the medium. It could be due to: the diversity of the natural community, the possibility to other bacteria than E. coli to grow on the used culture medium, the restrictiveness of the serum for "pathogenic...
Tipo: Text
Palavras-chave: Escherichia coli; Bacteria; Microbiological culture; Pathogenic bacteria; Microbial contamination; Bacterial counters; Fluorescence microscopy; Bacteria collecting devices; Indicator species.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1001.pdf

Bilan des analyses du réseau microbiologique (REMI), en Languedoc-Roussillon, d'avril 1989 à décembre 1991
Provedor de dados: 5
Autores: Comps, Marie-annick; Tournier, Henri; Sospedra, Colette.
Le réseau de surveillance microbiologique d'IFREMER a été mis en place dans toute la France en 1989, dans le but de garantir la santé publique et de suivre la qualité de l'environnement. Dans la région Languedoc-Roussillon sept sites producteurs de coquillages sont surveillés chaque mois ou plus fréquemment si· nécessaire. Dans le présent rapport, les méthodes d'analyses et les critères d'étude des données sont décrits; les résultats acquis d'avril 1989 à décembre 1991 sont présentés pour chaque site en essayant d'expliquer les contaminations observées par les sources de pollution potentielles recensées, la pluviométrie ou tout autre facteur local. Un traitement des données conduit à différentes conclusions : - En mer, où l'élevage des moules sur filière...
Tipo: Text
Palavras-chave: Réseau microbiologique; Languedoc-Roussillon; Escherichia coli; Salmonelles; Surveillance.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00418/52976/53904.pdf

Recherche de l'origine des sources de contamination microbiologique en rivière de Pénerf. Etat des lieux et proposition de suivi complémentaire
Provedor de dados: 5
Autores: Treguier, Cathy; Stanisiere, Jean-yves; Allenou, Jean-pierre; Manach, Soazig.
La qualité microbiologique des zones de production conchylicole de la rivière de Pénerf (Morbihan) se dégrade depuis 2005, avec l'apparition d'alertes régulières, déclenchées dans le cadre du réseau de suivi microbiologique des zones de production de coquillages (REMI) de l'Ifremer. Les sources potentielles de contamination présentes au niveau du bassin versant sont nombreuses avec la présence de 5 stations d'épuration y rejetant leurs effluents, de nombreux secteurs en assainissement individuel, des réseaux d'eaux pluviales potentiellement contaminés, deux entreprises agroalimentaires et 107 exploitations agricoles. En vue de préciser l'origine des contaminations dans ce secteur, les résultats acquis dans le cadre de trois réseaux de suivi (suivi des eaux...
Tipo: Text
Palavras-chave: Contamination fécale; Escherichia coli; Bassin versant; Pénerf; Sources de contamination; Qualité des zones de production conchylicoles.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00141/25176/23281.pdf

Résultats de la Surveillance de la Qualité du Milieu Marin Littoral - Départements : Charente- Maritime &amp; Vendée (sud) - Edition 2007
Provedor de dados: 5
Autores: Ifremer.
Le Laboratoire Environnement Ressources des Pertuis Charentais opère, sur le littoral des départements de Charente-Maritime et de Vendée (partie sud), les réseaux de surveillance nationaux de l’Ifremer dont une description succincte est présentée ci-dessous. Les résultats figurant dans ce bulletin sont obtenus à partir de données validées extraites de la base Ifremer Quadrige1 (base des données de la surveillance de l’environnement marin littoral): REMI Réseau de contrôle microbiologique, REPHY Réseau de surveillance du phytoplancton et des phycotoxines, RNO Réseau national d’observation de la qualité du milieu marin, REMORA Réseau mollusques des ressources aquacoles, RAZLEC Réseau de suivi hydrologique sur le Bassin de Marennes-Oléron
Tipo: Text
Palavras-chave: Surveillance pollution; Milieu marin; Réseau observation; Zone littorale; Phytoplancton; Metal lourd; Toxicité; Escherichia coli; Alexandrium; Dinophysis; Charente Maritime; Vendée Sud.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00041/15210/12538.pdf

Investigating an avian source of shellfish faecal contamination in the Thau lagoon (the Mediterranean, France)
Provedor de dados: 5
Autores: Derolez, Valerie; Serais, Ophelie; Caprais, Marie-paule; Le Saux, Jean-claude; Messiaen, Gregory.
Microbes regularly contaminate Thau lagoon shellfish. Between 1997 and 2007, 18% of the analyses carried out in dry weather recorded more than 230 Escherichia coli / 100 g of bivalves (Ifremer French microbial monitoring network), leading the administration to restrict the shellfish harvest area, including suspension of production. This study aims to determine, the contribution of seabirds, which roost at night on the Thau lagoon shellfish growing frames, to shellfish faecal pollution in dry weather.Data were collected in 2008 from both the drainage area (pollution input) and the lagoon (water quality and microbial monitoring, seabird counts) in dry weather. The data showed that, with no watershed input or lagoon resurgence, microbial contaminants were...
Tipo: Text
Palavras-chave: Escherichia coli; Origine aviaire; Coquillage; Contamination fécale; Étang de Thau; Oiseau de mer; Bactériophage; Dépistage de la source microbienne; Temps sec; Escherichia coli; Avian source; Shellfish; Faecal contamination; Thau lagoon; Seabird; Bacteriophage; Microbial source tracking; Dry weather.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00025/13593/10659.pdf

Contrasting effects of historical contingency on phenotypic and genomic trajectories during a two-step evolution experiment with bacteria
Provedor de dados: 5
Autores: Plucain, Jessica; Suau, Antonia; Cruveiller, Stephane; Medigue, Claudine; Schneider, Dominique; Le Gac, Mickael.
Background  The impact of historical contingency, i.e. the past evolutionary history of a population, on further adaptation is mostly unknown at both the phenotypic and genomic levels. We addressed this question using a two-step evolution experiment. First, replicate populations of Escherichia coli were propagated in four different environmental conditions for 1000 generations. Then, all replicate populations were transferred and propagated for further 1000 generations to a single new environment. Results  Using this two-step experimental evolution strategy, we investigated, at both the phenotypic and genomic levels, whether and how adaptation in the initial historical environments impacted evolutionary trajectories in a new environment. We showed that...
Tipo: Text
Palavras-chave: Experimental evolution; Escherichia coli; Adaptation; Historical contingency; Epistasis.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00333/44447/44118.pdf

Recherche de l'origine des sources de contamination microbiologique en rivière de Pénerf. Partie 2, Hiérarchisation des flux et modélisation de la contamination
Provedor de dados: 5
Autores: Treguier, Cathy; Hitier, Benoist.
Dans le cadre de la recherche de l’origine des contaminations en rivière de Penerf, une étude a été menée en partenariat entre l’Ifremer et le SIAGM de juillet 2010 à décembre 2011. Des échantillons d’eau douce aux 8 principaux exutoires, ainsi que des coquillages ont été prélevés à une fréquence bimensuelle afin de dénombrer les indicateurs de contamination fécale Escherichia coli. Le calcul des débits au niveau du bassin versant a permis de reconstituer les flux de bactéries fécales provenant des exutoires étudiés. La contamination des points de prélèvement coquillages situés plus en amont s’avère être supérieure, en moyenne, à la contamination des points REMI situés en aval. Cependant, aucune relation statistique significative n’a pu être mise en...
Tipo: Text
Palavras-chave: Contamination fécale; Escherichia coli; Bassin versant; Pénerf; Flux; Qualité des zones de production conchylicoles; Modélisation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00140/25174/23280.pdf

VIBRance : éValuation des Impacts Bactériologiques dans l'Estuaire de la Rance. Hiérarchisation des flux de contamination microbiologique dans le cadre de l’élaboration du profil de vulnérabilité conchylicole du secteur Rance et baie de Saint-Malo
Provedor de dados: 5
Autores: Cheve, Julien; Le Noc, Sandrine.
Le projet VIBRance (éValuation des Impacts Bactériologiques dans l’estuaire de la Rance) a fait suite à l’observation d’une nette dégradation de la qualité microbiologique des eaux dans l’estuaire de la Rance (Rance maritime &amp; baie de Saint-Malo) au milieu des années 2010, présentant un risque pour la pérennité des activités tributaires : pêche à pied de loisir et professionnelle, conchyliculture, algoculture et à plus long terme la baignade. La situation revêt un caractère multi-sources (nombreux exutoires) et complexe (hydrodynamisme d’un estuaire découpé et contrôlé par un barrage marémoteur). La problématique posée est quelles sont les sources de contamination microbiologique dans l’estuaire de la Rance et leurs impacts associés ? Le projet...
Tipo: Text
Palavras-chave: Impact; Bactériologique; Microbiologique; Estuaire; Rance; Baie de Saint- Malo; Profil; Vulnérabilité; Conchylicole; Baignade; Pêche; Pied; Escherichia coli; Diagnostic; Courantologie; Modélisation; Dispersion source; Contributeur; VIBRance.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00442/55363/56879.pdf

Qualité du Milieu Marin Littoral - Bulletin de la surveillance - Edition 2011 - Départements : Charente- Maritime &amp; Vendée (Sud)
Provedor de dados: 5
Autores: Ifremer.
L’Ifremer met en oeuvre, à l’échelle de l’ensemble du littoral métropolitain, une surveillance de la qualité du milieu marin côtier pour répondre aux objectifs environnementaux de la Directive Cadre sur l’Eau (DCE), aux obligations des conventions régionales marines (OSPAR et Barcelone) et aux objectifs sanitaires réglementaires concernant le suivi de la salubrité des coquillages des zones de pêche et de production conchylicoles. Cette surveillance s’appuie sur plusieurs réseaux de surveillance : le réseau de contrôle microbiologique (REMI), le réseau de surveillance du phytoplancton et des phycotoxines (REPHY), le réseau d’observation de la contamination chimique (ROCCH) et le réseau de surveillance benthique (REBENT). Ces réseaux sont mis en oeuvre par...
Tipo: Text
Palavras-chave: Surveillance pollution; Milieu marin; Réseau observation; Zone littorale; Phytoplancton; Metal lourd; Toxicité; Escherichia coli; Alexandrium; Dinophysis; Charente Maritime; Vendée Sud.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00041/15225/12542.pdf

Etude sanitaire de la zone des Sableaux (85) – groupes 2 et 3
Provedor de dados: 5
Autores: Rocq, Sophie.
L’étude sanitaire de la zone des Sableaux a été menée en réponse à la demande de classement de cette zone par le Comité Régional des Pêches et des Elevages Marins des Pays de la Loire, pour les groupes 2 (coquillages fouisseurs) et 3 (coquillages non fouisseurs). Une étude de dossier a été réalisée afin de recenser les principales sources de contamination microbiologique et d’évaluer leurs impacts sur la zone étudiée. Deux points de prélèvement de coquillages (un pour les palourdes, un pour les moules) ont été échantillonnés deux fois par mois entre février 2019 et janvier 2020, en vue de l’analyse de leur concentration en Escherichia coli (indicateur de contamination fécale). Les résultats de ces analyses permettent d’estimer en B la qualité de chacun des...
Tipo: Text
Palavras-chave: Escherichia coli; Étude sanitaire; Vendée; Classement sanitaire; Contamination bactériologique des coquillages; Moules; Palourdes; Fouisseurs; Non fouisseurs; Noirmoutier; Les Sableaux.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00618/73023/72100.pdf

Adaptative responses of E-coli to marine environmental stresses. a modelling approach based on viability and dormancy concepts
Provedor de dados: 5
Autores: Martin, Y; Troussellier, Marc; Bonnefont, Jl.
In an attempt to synthesize conceptual and experimental information on the behaviour of enteric bacteria in seawater, a mathematical model has been developed. This model is based on changes in intracellular metabolisable components depending an physiological responses of the cell when subjected to nutrient starvation, salinity stress and solar radiation. Following a strategy which takes into account short- and mid-term physiological adaptation and reversal processes, the cells can develop in different states: culturable (B-1), viable and definitively nonculturable (B-2), and reversibly dormant (B-3) cells. Model parameters were deduced either from original microcosm experiments, from literature data or calibration procedures. Validation of the model was...
Tipo: Text
Palavras-chave: Escherichia coli; Eau de mer; Stress; États cellulaires; Modélisation; Escherichia coli; Seawater; Stress; Cellular states; Modelling.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00325/43646/43224.pdf

Norwalk virus-specific binding to oyster digestive tissues
Provedor de dados: 5
Autores: Le Guyader, Soizick; Loisy, Fabienne; Atmar, R; Hutson, A; Estes, M; Ruvoen-clouet, N; Pommepuy, Monique; Le Pendu, J.
The primary pathogens related to shellfishborne gastroenteritis outbreaks are noroviruses. These viruses show persistence in oysters, which suggests an active mechanism of virus concentration. We investigated whether Norwalk virus or viruslike particles bind specifically to oyster tissues after bioaccumulation or addition to tissue sections. Since noroviruses attach to carbohydrates of the histo-blood group family, tests using immunohistochemical analysis were performed to evaluate specific binding of virus or viruslike particles to oyster tissues through these ligands. Viral particles bind specifically to digestive ducts (midgut, main and secondary ducts, and tubules) by carbohydrate structures with a terminal N-acetylgalactosamine residue in an alpha...
Tipo: Text
Palavras-chave: Bioaccumulation; Shellfish; Escherichia coli; Pathogen; Oyster digestive tissue; Noroviruses.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1542.pdf

Etude sanitaire des zones 22.04.11, 22.06.12 et 22.06.13 entre la baie de Paimpol et l'île de Bréhat - groupe III
Provedor de dados: 5
Autores: Cheve, Julien; Le Noc, Sandrine.
Ce rapport présente l’évaluation de la qualité microbiologique et chimique des zones de production conchylicole 22.04.11 « Baie de Paimpol Sud », 22.06.12 « Ilots de Bréhat Sud » et 22.06.13 « Lanmodez, ilots de Bréhat Ouest ». La qualité globale des zones est bonne, présentant entre 96% et 99 % de résultats inférieurs à 230 E.coli / 100g CLI . Mais chacune d’entre elles présente un pic de moyenne qualité microbiologique sur la période de l’étude, qui les déclasse en B. Bien que d’occurences rares, ces contaminations peuvent difficilement être considérées comme exceptionnelles. Les conclusions de l’étude sont :  - Zone 22.04.11 « Baie de Paimpol Sud » : La qualité sanitaire estimée est B, les points de suivi à maintenir sont « Port Lazo » et « Kérarzic...
Tipo: Text
Palavras-chave: Etude sanitaire; Escherichia coli; Contamination bactériologique des coquillages; Contaminants chimiques; Milieu marin; Zones de production; Baie de Paimpol; Ile de Bréhat; Lanmodez; Département 22; Côtes d’Armor; Groupe III; Huîtres.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00586/69794/67689.pdf

Evaluation de la qualité des zones de production conchylicole : Département : Gironde - Edition 2006
Provedor de dados: 5
Autores: Cantin, Christian; Neaud-masson, Nadine; Mayeur, Didier.
After a reminder of the objectives, operation and method of interpreting the results of the REMI microbiological monitoring network, this report establishes a national assessment and describes the annual programme of the Gironde region. It presents all of the results obtained, in particular the assessment of the quality of listed shellfish production areas and the evolution of their quality.
Tipo: Text
Palavras-chave: Escherichia coli; Contamination; Microbiology; Environment quality; Shellfish culture; Département de la Gironde; Classement sanitaire des zones de production; Contamination bactériologique des coquillages; Escherichia coli; REMI.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-2102.pdf

Etude sanitaire du secteur des Gastines - Rance – Groupe II
Provedor de dados: 5
Autores: Cheve, Julien; Le Noc, Sandrine.
Ce rapport présente l’évaluation de la qualité microbiologique et chimique dans le secteur des Gastines en Rance (Bras de Châteauneuf) pour le groupe II (coques et palourdes) en vue de son classement sanitaire. L’étude s’est déroulée entre février 2018 et mars 2019, avec l’obtention de 49 résultats microbiologiques (dénombrement des Escherichia coli dans 100 g de CLI) en deux points : « Les Gastines » (021-P-111) et « Les Guettes » (021-P-112) et la recherche de molécules chimiques (Pb, Hg, Cd, PCB, PCB dl, dioxines, furanes et HAP par kg de chair humide), au point « Ville Ger » (021-P-005). Les données de l’étude, tant sur les coquillages échantillonnés que sur l’analyse des sources de contamination et les simulations hydrodynamiques réalisées, ont montré...
Tipo: Text
Palavras-chave: Etude sanitaire; Escherichia coli; Contamination bactériologique des coquillages; Contaminants chimiques; Milieu marin; Zones de production; Estuaire de la Rance; Gisement des Gastines; Bras de Châteauneuf; Département 35; Ille-et-Vilaine; Groupe II; Palourde; Coque.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00500/61207/64752.pdf

Etude sanitaire pour le classement de la zone n° 29*07.04 – Rivière de Pont l’Abbé (Finistère). Coquillages fouisseurs
Provedor de dados: 5
Autores: Boulben, Sylviane; Raguenes, Pierre.
Lors de la réunion de la Commission de suivi du classement sanitaire des zones de production de coquillages marins vivants du 12 avril 2000, département du Finistère, la zone conchylicole n° 29.07.04, de la rivière de Pont l’Abbé a été classée C pour les bivalves non fouisseurs (groupe 3). Afin de classer cette zone pour les coquillages du groupe 2, le Directeur Départemental des Affaires Maritimes a sollicité Ifremer pour la réalisation d’une étude du niveau de salubrité entre septembre 2000 et octobre 2001. Les résultats bactériologiques indiquent une contamination de niveau D. Les résultats disponibles des paramètres chimiques ont mis en évidence des teneurs qui correspondent à un classement de niveau A.
Tipo: Text
Palavras-chave: Classement de salubrité; Escherichia coli; Coquillages fouisseurs; Rivière de Pont l’Abbé.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00327/43774/43331.pdf

Qualité du Milieu Marin Littoral - Bulletin de la surveillance - Edition 2008 - Départements : Charente- Maritime &amp; Vendée (Sud)
Provedor de dados: 5
Autores: Ifremer.
Le Laboratoire Environnement Ressources des Pertuis Charentais opère, sur le littoral des départements de Charente-Maritime et de Vendée (partie sud), les réseaux de surveillance nationaux de l’Ifremer dont une description succincte est présentée ci-dessous. Les résultats figurant dans ce bulletin sont obtenus à partir de données validées extraites de la base Ifremer Quadrige1 (base des données de la surveillance de l'environnement marin littoral), données recueillies jusqu’en 2007 : REMI Réseau de contrôle microbiologique, REPHY Réseau de surveillance du phytoplancton et des phycotoxines, ROCCH Réseau d’observation de la contamination chimique, REMORA Réseau mollusques des ressources aquacoles, REMOULA Réseau régional de suivi de la croissance des moules,...
Tipo: Text
Palavras-chave: Surveillance pollution; Milieu marin; Réseau observation; Zone littorale; Phytoplancton; Metal lourd; Toxicité; Escherichia coli; Alexandrium; Dinophysis; Charente Maritime; Vendée Sud.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00041/15212/12539.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine. Sous-région marine Méditerranée occidentale. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Amouroux, Isabelle.
Tipo: Text
Palavras-chave: Méditerranée; Coquillages; Escherichia coli; Réglementation; Qualité microbiologique; Evolution.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00331/44243/43812.pdf

The behaviour of F specific bacteriophage in depurating shellfish with reference to their use as pollution indicator organisms
Provedor de dados: 5
Autores: Lees, D; Dore, W.
The behaviour of sex-pilli specific (F+) bacteriophage during the depuration process was investigated using Pacific oysters (Crassostrea gigas) and edible mussels (Mytilus edulis) naturally contaminated with untreated sewage. F+ bacteriophage was eliminated considerably slower than E. coli. This effect was most pronounced in oysters which showed average T90 bacteriophage values of 62.5 hours compared with 11 hours for E. coli. F+ bacteriophage in mussels was largely confined to the digestive tract. The majority of E. coli were similarly located but, in contrast, counts were also distributed throughout the other internal tissues. Investigation of ultra-violet (UV) dosage showed that the difference in depuration rate was unlikely to be due to the higher...
Tipo: Text
Palavras-chave: Escherichia coli; Mytilus edulis; Crassostrea gigas; Bivalvia; Indicator species; Marine molluscs; Bacteriophages; Pollution indicators; Marine pollution; Shellfish; Self purification.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1605.pdf

Etude sanitaire de la zone expérimentale entre Saint-Aubin-sur-Mer et Quiberville (Seine-Maritime) - groupe 3 (coquillages bivalves non fouisseurs)
Provedor de dados: 5
Autores: Rocq, Sophie.
Suite à la demande du Comité Régional de la Conchyliculture Normandie-Mer du Nord,la Direction Départementale des Territoires et de la Mer de Seine-Maritime a demandé la réalisation d’une étude sanitaire en vue du classement de la zone d’estran entre Saint-Aubin-sur-Mer et Quiberville, qui fait actuellement l’objet d’une expérimentation pour le développement de l’activité ostréicole sur ce site. Une étude de dossier et une inspection du littoral ont été réalisées afin de recenser les principales sources de contamination microbiologique et d’évaluer leurs impacts sur la zone étudiée. Ce travail a suggéré que le risque de contamination microbiologique était a priori plus important dans la partie est de la concession expérimentale. Deux points de prélèvement...
Tipo: Text
Palavras-chave: Etude sanitaire; Escherichia coli; Seine-Maritime; Classement sanitaire; Contamination bactériologique des coquillages; Huîtres; Non fouisseurs; Saint-Aubin-sur-Mer; Quiberville.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00656/76778/77936.pdf

Le bassin versant du Bélon : vers une restauration durable de la qualité bactériologique des eaux estuariennes
Provedor de dados: 5
Autores: Monfort, Patrick; Hervio Heath, Dominique; Caprais, Marie-paule; Pommepuy, Monique; Annezo, Jean-pierre; Loaec, Solen; Le Mennec, Cecile; Guillerm, Emmanuelle; Boulben, Sylviane; Bilien, Gwenael; Bonsor, Robert; Porter, Jonathan; Pickup, Roger.
Le projet européen Cycleau, initié à l'échelle locale sur la problèmatique de l'envasement et de l'ensablement de l'estuaire du Bélon, y a également intégré un volet bactériologique en raison des contraintes fortes que faisaient peser les pics conjoncturels de contamination sur la profession conchylicole (fermetures temporaires, déclassement éventuel) mais plus largement sur l'image du territoire (impact touristique) et en définitive sur son développement durable. Pour satisfaire la restauration de la qualité des eaux estuariennes, l'IFREMER a fait appel à un certain nombre d'outils susceptibles de contribuer à la formulation d'un diagnostic pertinent et ainsi de répondre aux préoccupations des acteurs du littoral. Pour ce faire, nous avons créé un réseau...
Tipo: Text
Palavras-chave: Bassin versant; Contamination fécale; Escherichia coli; Flux bactériens; Bactériophages; SIG; Évaluation.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00200/31077/29490.pdf

Etude de zone microbiologique. Ria de la rivière du Faou
Provedor de dados: 5
Autores: Monfort, Patrick; Lebrun, Luc.
L'activité conchylicole sur la rivière du Faou se résume pour l'heure à l'élevage d'huîtres creuses pour lequel l'estuaire est classé en zone de salubrité B. Suite à la sollicitation du Comité Régional des Pêches Maritimes et des Elevages Marins (CRPMEM) de Bretagne pour y entreprendre une étude sanitaire sur les coquillages du groupe 2, l'administration départementale a donné un avis favorable et missionné l'Institut Français de Recherche pour l'Exploitation de la MER (IFREMER) pour mener à bien cette étude. Ce rapport préliminaire ne traite que les données bactériologiques et chimiques des coquillages acquises dans le cadre du suivi sanitaire et ceci dans un souci d'une transmission rapide de l'information aux acteurs concernés. Le second volet relatif...
Tipo: Text
Palavras-chave: Rivière du Faou; Contamination fécale; Escherichia coli; Plomb; Cadmium; Mercure; Dioxines; Polychlorobiphényles; Benzo(a)pyrène; Zone conchylicole; Palourdes.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00195/30650/29121.pdf

Etude sanitaire pour le classement de la zone n° 29*02.04 - Anse de Brouennou (Finistère). Coquillages fouisseurs
Provedor de dados: 5
Autores: Boulben, Sylviane; Rocher, Gregory.
Lors de la réunion de la Commission de suivi du classement sanitaire des zones de production de coquillages marins vivants du 12 avril 2000, département du Finistère, la zone conchylicole n° 29.02.04, Anse de Brouennou, a été classée B pour les bivalves non fouisseurs (groupe 3). Afin de classer cette zone pour les coquillages du groupe 2, le Directeur Départemental des Affaires Maritimes a sollicité Ifremer pour la réalisation d’une étude du niveau de salubrité entre septembre 2000 et octobre 2001. Les résultats bactériologiques indiquent une contamination de niveau B. Les résultats disponibles des paramètres chimiques ont mis en évidence des teneurs qui correspondent à un classement de niveau A.
Tipo: Text
Palavras-chave: Classement de salubrité; Escherichia coli; Coquillages fouisseurs; Anse de Brouennou.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00327/43771/43328.pdf

Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par Escherichia coli. Sous-région marine Manche - Mer du Nord. Evaluation initiale DCSMM.
Provedor de dados: 5
Autores: Amouroux, Isabelle.
Tipo: Text
Palavras-chave: Manche; Mer du Nord; Coquillages; Contamination; Escherichia coli; Réglementation; Qualité microbiologique; Evolution.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00328/43873/43447.pdf

Etude sanitaire de la zone 50.24 « baie du Mont-Saint-Michel » (partie nord) - groupe 2 (coquillages bivalves fouisseurs)
Provedor de dados: 5
Autores: Rocq, Sophie.
Suite à des problèmes de qualité bactériologique et un manque de ressource au point de suivi REMI à Saint-Jean-le-Thomas, la Direction Départementale des Territoires et de la Mer (DDTM) a demandé la réalisation d’une étude sanitaire en vue du découpage de la zone 50.24 « baie du Mont-Saint-Michel » pour le groupe 2. Etant donné que la partie sud de la zone 50.24 est interdite à la pêche professionnelle par arrêté préfectoral du 21/12/2017 et que les gisements de coquillages fouisseurs exploitables par les professionnels n’y sont plus présents à l’heure actuelle, la présente étude se concentre sur la partie nord de la zone 50.24. Une étude de dossier et une inspection du littoral ont été réalisées afin de recenser les principales sources de contamination...
Tipo: Text
Palavras-chave: Etude sanitaire; Escherichia coli; Manche; Classement sanitaire; Contamination bactériologique des coquillages; Coques; Palourdes; Fouisseurs; Mont-Saint-Michel; Champeaux.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00689/80134/83181.pdf

Evaluation de la qualité des zones de production conchylicole. Département de Loire-Atlantique. Edition 2020
Provedor de dados: 5
Autores: Schmitt, Anne; Piquet, Jean-come; Cochennec-laureau, Nathalie.
Après un rappel des objectifs, du fonctionnement et la méthode d’interprétation desrésultats, ce rapport décrit les résultats obtenus en 2019 pour le réseau de surveillance de la contamination microbiologique REMI et du réseau d’observation de la contamination chimique ROCCH du département de Loire-Atlantique. Il présente l’estimation de la qualité microbiologique et chimique des zones de production de coquillages classées. En 2019, il y a eu 29 alertes déclenchées : 26 de niveau 0, 2 de niveau 1 et 1 de niveau 2. Sur 27 zones classées, 4 zones présentes des discordances de classement et une zone n’a pas suffisamment de données pour être classée. Sur les 4 zones dont le classement sanitaire ne concorde pas avec l’estimation de la qualité, 2 sont classées...
Tipo: Text
Palavras-chave: Ifremer; REMI; Escherichia coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Estimation de la qualité sanitaire des zones de production; Département de Loire-Atlantique.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00624/73621/73059.pdf

Etude sanitaire microbiologique. Zone aval de la ria de l'Odet
Provedor de dados: 5
Autores: Monfort, Patrick.
L'activité conchylicole emblématique de l'estuaire de l'Odet était tournée pendant des décennies vers l'ostréiculture et tout particulièrement l'exploitation du gisement d'huîtres plates du Perennou. Les mortalités importantes enregistrées dans les années 70 et 80 liées à la présence des parasites Martelia et Bonamia, associées à la médiocrité de la qualité sanitaire des eaux estuariennes ont participé au déclin de cette activité primaire sur ce territoire. Une volonté locale forte au sein de la Commission Locale de l'Eau (CLE) du Schéma d'Aménagement et de Gestion des Eaux (SAGE) de réhabiliter la qualité bactériologique en priorité puis corrélativement l'activité conchylicole ont contribué au regain d'intérêt de cet estuaire manifesté par les...
Tipo: Text
Palavras-chave: Contamination fécale; Escherichia coli; Plomb; Cadmium; Mercure; Zone conchylicole; Ceratoculture; Odet.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00175/28616/27044.pdf

Etude sanitaire de la partie nord-ouest de la zone 44.09 « Estuaire de la Loire » - groupe 3 (bivalves non fouisseurs)
Provedor de dados: 5
Autores: Rocq, Sophie.
Suite à la pérennisation de l’exploitation d’un gisement d’huîtres creuses classé en zone à exploitation occasionnelle (dite « zone à éclipse »), la Direction Départementale des Territoires et de la Mer (DDTM) de Loire-Atlantique a demandé la réalisation d’une étude sanitaire en vue du classement pérenne de ce gisement se trouvant dans la partie nordouest de la zone 44.09 « Estuaire de la Loire ». Une étude de dossier et une inspection du littoral ont été réalisées afin de recenser les principales sources de contamination microbiologique et d’évaluer leurs impacts sur la zone étudiée. Ce travail préalable a permis de définir l’étendue du gisement côtier dont la qualité microbiologique pouvait être représentée par le point de suivi déjà existant 070-P-081...
Tipo: Text
Palavras-chave: Escherichia coli; Étude sanitaire; Loire-Atlantique; Classement sanitaire; Contamination bactériologique des coquillages; Huîtres; Non fouisseurs; Estuaire de la Loire; Loire.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00635/74738/74798.pdf

Etude sanitaire microbiologique - Site de la Baie de Goulven
Provedor de dados: 5
Autores: Monfort, Patrick; Lebrun, Luc.
Sur le plan conchylicole, la baie de Goulven est exclusivement concernée par les gisements de coques, aucune activité de production de coquillages n’y étant pratiquée. Le Comité Régional des Pêches Maritimes et des Elevages Marins (CRPMEM), après avoir engagé une évaluation du stock de bivalves par un bureau d’études (Allain et Bernard 2015), a sollicité la Direction Départementale des Territoires et de la Mer (DDTM) du Finistère pour entreprendre une étude sanitaire et permettre ainsi le classement sanitaire de cette zone et son accès pour les pêcheurs à pied professionnels. Ayant répondu favorablement à cette demande de la profession, l’administration a missionné le laboratoire Ifremer de Concarneau pour mener à bien cette étude. Les résultats obtenus...
Tipo: Text
Palavras-chave: Baie de Goulven; Contamination fécale; Escherichia coli; Plomb; Cadmium; Mercure; Dioxines; Polychlorobiphényles; Benzo(a)pyrène; Zone conchylicole; Coques.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00369/48023/48109.pdf

Etude sanitaire de la rivière Laïta - Zones 2956.08.100 et 56.08.100 - Groupes 2 et 3
Provedor de dados: 5
Autores: Boulben, Sylviane.
Longtemps classée C par l’arrêté préfectoral finistérien pour les coquillages du groupe 3, la zone 2956.08.100 (n° 56.08.100 par arrêté préfectoral du Morbihan) atteint une qualité sanitaire de niveau B et un classement B depuis 26/10/2004 [1]. La présence de coques en quantité substantielle et le souci de diversification de la production ont conduit les professionnels de l’établissement mytilicole Romagné à solliciter la création de concessions pour y élever des coques (Cerastoderma edule), coquillages fouisseurs du groupe 2. Afin de s’assurer de la qualité sanitaire de ces bivalves fouisseurs, une étude sanitaire a été initiée à la demande des représentations professionnelles. Par ailleurs, afin de répondre à l’obligation réglementaire de réaliser ces...
Tipo: Text
Palavras-chave: Etude sanitaire; Escherichia coli; Contamination bactériologique des coquillages; Contaminants chimiques; Milieu marin; Zone de production conchylicole; Finistère; Morbihan; Rivière de La Laïta; Coques; Moules; Huîtres; Gisement; Groupes 2 et 3.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00677/78911/81272.pdf

Etude sanitaire de la zone 3522.01 - Rance Nord - Groupe III
Provedor de dados: 5
Autores: Cheve, Julien; Le Noc, Sandrine.
Ce rapport présente l'évaluation de la qualité microbiologique et chimique dans la partie Nord de la Rance maritime (zone 3522.01) pour le groupe III (coquilles Saint-Jacques) en vue de son classement sanitaire. Spécifiquement conduite pour les coquilles Saint-Jacques (Pecten maximus), c'est la première étude sanitaire réalisée pour ce taxon, s'agissant d'un secteur susceptible de subir des contaminations microbiologiques et en l'absence d'autres suivis REMI disponibles. La répartition des résultats dans les différentes classes de qualité sanitaire permettrait d'estimer en "B" (qualité moyenne) la qualité microbiologique de l'ensemble de la zone 3522.01. Concernant la qualité chimique, les coquiiles Saint-Jacques présentent un dépassement du seuil...
Tipo: Text
Palavras-chave: Etude sanitaire; Escherichia coli; Contamination bactériologique des coquillages; Contaminants chimiques; Milieu marin; Zones de production; Estuaire de la Rance; Ile Chevret; Département 35; Ille-et-Vilaine; Groupe III; Coquille Saint-Jacques.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00502/61333/64909.pdf

Etude sanitaire pour le classement de la zone n° 29*08.04 – Rivière de l’Aven (Finistère). Coquillages fouisseurs
Provedor de dados: 5
Autores: Boulben, Sylviane; Le Gal, Dominique.
vivants du 12 avril 2000, département du Finistère, la zone conchylicole n° 29*08.04, de la rivière de l’Aven a été classée B pour les bivalves non fouisseurs (groupe 3). Afin de classer cette zone pour les coquillages du groupe 2, le Directeur Départemental des Affaires Maritimes a sollicité Ifremer pour la réalisation d’une étude du niveau de salubrité entre septembre 2000 et novembre 2001. Les résultats bactériologiques indiquent une contamination de niveau C. Les résultats disponibles des paramètres chimiques ont mis en évidence des teneurs qui correspondent à un classement de niveau A.
Tipo: Text
Palavras-chave: Classement de salubrité; Escherichia coli; Coquillages fouisseurs; Rivière de l’Aven.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00327/43772/43329.pdf

Etude sanitaire pour le classement de la zone n° 29*04.04 – Rivière de l’Elorn (Finistère). Coquillages fouisseurs
Provedor de dados: 5
Autores: Boulben, Sylviane; Rocher, Gregory.
Lors de la réunion de la Commission de suivi du classement sanitaire des zones de production de coquillages marins vivants du 12 avril 2000, département du Finistère, la zone conchylicole n° 29.04.04, de l’estuaire de l’Elorn a été classée B pour les bivalves non fouisseurs (groupe 3). Afin de classer cette zone pour les coquillages du groupe 2, le Directeur Départemental des Affaires Maritimes a sollicité Ifremer pour la réalisation d’une étude du niveau de salubrité entre septembre 2000 et octobre 2001. Les résultats bactériologiques indiquent une contamination de niveau B. Les résultats disponibles des paramètres chimiques ont mis en évidence des teneurs qui correspondent à un classement de niveau A.
Tipo: Text
Palavras-chave: Classement de salubrité; Escherichia coli; Coquillages fouisseurs; Rivière de l’Elorn.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00327/43773/43330.pdf

Evaluation de la qualité des zones de production conchylicole. Département : Loire-Atlantique. Edition 2018
Provedor de dados: 5
Autores: Schmitt, Anne; Cochennec-laureau, Nathalie; Piquet, Jean-come.
Après un rappel des objectifs, du fonctionnement et de la méthode d’interprétation des résultats, ce rapport décrit les résultats obtenus en 2017 pour le réseau de surveillance de la contamination microbiologique REMI et du réseau d’observation de la contamination chimique ROCCH du département de Loire–Atlantique. Il présente l’estimation de la qualité microbiologique et chimique des zones de production de coquillages classées.
Tipo: Text
Palavras-chave: Ifremer; REMI; Escherichia coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Estimation de la qualité sanitaire des zones de production; Département de Loire-Atlantique.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00440/55198/56809.pdf

Zones conchylicoles de l'Aven, du Bélon et du Merrien : Bilan de 15 ans de surveillance
Provedor de dados: 5
Autores: Monfort, Patrick; Nicol, P.
L’activité conchylicole sur les estuaires du Sud Est Cornouaille en Finistère est un enjeu majeur en raison de son importance socio-économique à l’échelle locale. Cette filière est soumise a de multiples contraintes et notamment au respect de la qualité sanitaire des produits finis,  à l’origine de potentielles Toxi-Infections Alimentaires (TIA) qui peuvent fragiliser sa pérennité. La qualité microbiologique des eaux littorales est donc un point essentiel à maîtriser pour limiter les risques sanitaires liés à la consommation de ces fruits de la mer. Pour ce faire, différents moyens ont été mis en place, qu’il s’agisse de la réglementation des zones conchylicoles à l’échelon national ou de l’élaboration d’actions de restauration de la qualité des eaux à...
Tipo: Text
Palavras-chave: Rivière de l’Aven; Rivière du Bélon; Rivière de Merrien; Contamination fécale; Escherichia coli; Plomb; Cadmium; Mercure; PCB; Dioxines; HAP; Ostréiculture.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00388/49955/50528.pdf

Organisation d'essais interlaboratoires sur coquillages vivants pour E. coli
Provedor de dados: 5
Autores: Garry, Pascal; Dupont, Jacques; Kaelin, Gaelle; Kergaravat, Cedric; Menanteau, Chantal; Vallade, Emilie; Veron, Antoine; Catherine, Martial.
En microbiologie des aliments, le document LAB REF 02 du cofrac demande aux laboratoires de participer au moins deux fois par an aux comparaisons interlaboratoires organisés dans leur domaine analytique afin de démontrer leur compétence et garantir ainsi la qualité de leurs résultats. Par ailleurs, le règlement CE n°882/2004 du 29 avril 2004 relatif aux contrôles officiels précise dans son article 33 que les laboratoires nationaux de référence organisent « des essais comparatifs entre les différents laboratoires nationaux officiels et assurent un suivi adéquat de ces essais ». Dans ce cadre le Laboratoire National de Référence (LNR) microbiologie des coquillages a mis en place des essais d’intercomparaison destinés à l’ensemble des laboratoires réalisant...
Tipo: Text
Palavras-chave: Microbiologie; Analyses microbiologiques; Coquillages; Essais interlaboratoires; Escherichia coli.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00143/25473/23627.pdf

Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi
Provedor de dados: 5
Autores: Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques.
This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively....
Tipo: Text
Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf

Traitement de l''eau par ultraviolets, application a la purification des coquillages
Provedor de dados: 5
Autores: Cailleres, J.
The germicide properties of ultra-violet radiation in a wave length ranging from 100 to 400 mm were already discovered back in the late 19th century. These properties specific to UV-C radiation (200-280 mm) result from its action on the nucleic acids of the micro-organisms. Each micro-organism, depending on its composition, therefore presents a specific sensitivity to this radiation.
Tipo: Text
Palavras-chave: France; Escherichia coli; Crassostrea; Bivalvia; Bacteria; Ultraviolet radiation; Self purification; Shellfish culture.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1629.pdf

Etude sanitaire des zones 50.14 « Gouville- Blainville », 50.14.01 « Gouville » et 50.14.02 « Blainville » - groupes 2 et 3 (coquillages bivalves fouisseurs et non fouisseurs)
Provedor de dados: 5
Autores: Rocq, Sophie.
La Direction Départementale des Territoires et de la Mer (DDTM) de la Manche a demandé la réalisation d’une étude sanitaire pour la zone 50.14 « Gouville-Blainville », qui a été classée pour le groupe 2 (coquillages bivalves fouisseurs) mais sans étude préalable. Cette étude a été élargie au groupe 3 (coquillages bivalves non fouisseurs) pour les zones 50.14.01 « Gouville » et 50.14.02 « Blainville » qui recouvrent le même secteur géographique et qui n’ont jamais fait l’objet d’une étude sanitaire non plus. Une étude de dossier et une inspection du littoral ont été réalisées afin de recenser les principales sources de contamination microbiologique et d’évaluer leurs impacts sur la zone étudiée. Ce travail a suggéré que le risque de contamination...
Tipo: Text
Palavras-chave: Escherichia coli; Étude sanitaire; Manche; Classement sanitaire; Contamination bactériologique des coquillages; Coques; Palourdes; Moules; Huîtres; Fouisseurs; Non fouisseurs; Blainville; Gouville.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00686/79818/82619.pdf

Factors Involved in the Persistence of a Shiga Toxin-Producing Escherichia coil O157:H7 Strain in Bovine Feces and Gastro-Intestinal Content
Provedor de dados: 5
Autores: Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne.
Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in...
Tipo: Text
Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf

Identification of the origin of faecal contamination in estuarine oysters using Bacteroidales and F-specific RNA bacteriophage markers
Provedor de dados: 5
Autores: Mieszkin, Sophie; Caprais, Marie-paule; Le Mennec, Cecile; Le Goff, Manon; Edge, T. A.; Gourmelon, Michele.
Aims: The aim of this study was to identify the origin of faecal pollution impacting the Elorn estuary (Brittany, France) by applying microbial source tracking (MST) markers in both oysters and estuarine waters. Methods and Results: The MST markers used were as follows: (i) human-, ruminant- and pig-associated Bacteroidales markers by real-time PCR and (ii) human genogroup II and animal genogroup I of F-specific RNA bacteriophages (FRNAPH) by culture/genotyping and by direct real-time reverse-transcriptase PCR. The higher occurrence of the human genogroup II of F-specific RNA bacteriophages using a culture/genotyping method, and human-associated Bacteroidales marker by real-time PCR, allowed the identification of human faecal contamination as the...
Tipo: Text
Palavras-chave: Escherichia coli; Estuarine and river waters; F-specific RNA bacteriophages; Host-associated Bacteroidales markers; Microbial source tracking; Shellfish.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00153/26426/25250.pdf

Etude de zone microbiologique. Ria de la rivière de Morlaix
Provedor de dados: 5
Autores: Monfort, Patrick; Lebrun, Luc.
La rivière de Morlaix est traditionnellement et exclusivement tournée vers la production d'huîtres creuses depuis des décennies. Dans ce contexte d'activité conchylicole, elle fait l'objet d'un classement sanitaire pour les coquillages du groupe 3 pris par l'arrêté préfectoral n° 2012 2361-0003 en date du 26/12/2012. Le Comité Régional de la Conchyliculture (CRC) et le Comité Régional des Pêches Maritimes et des Elevages Marins (CRPMEM) ont conjointement sollicité la Direction Départementale des Territoires et de la Mer (DDTM) pour entreprendre une étude sanitaire sur les coquillages du groupe 2 (coques et palourdes). Cette demande s'inscrit dans un double objectif de diversification de la production conchylicole dans un contexte difficile de mortalités...
Tipo: Text
Palavras-chave: Rivière de Morlaix; Contamination fécale; Escherichia coli; Plomb; Cadmium; Mercure; Dioxines; Polychlorobiphényles; Benzo(a)pyrène; Zone conchylicole; Palourdes.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00238/34949/33337.pdf

Inocuidad de carne molida y presencia de cepas Escherichia coli causantes de lesiones de adherencia y esfacelación*
Provedor de dados: 78
Autores: Srednik,M.E.T; Rumi,M.V.; Bentancor,A.
Escherichia coli es un marcador de contaminación fecal en alimentos. Dentro de la especie se encuentran patotipos de riesgo para la salud. Entre ellos, E. coli enteropatógeno y enterohemorrágico tienen la habilidad de causar la lesión de adherencia y esfacelación (A/E) en células intestinales. El estudio de ambos patotipos engloba aquellos más frecuentemente relacionados a diarrea infantil. El objetivo del presente trabajo fue evaluar la presencia de cepas A/E, particularmente las enteropatógenas que serían consideradas inocuas bajo la normativa de control actual. Se analizaron 98 muestras de carne molida compradas en carnicerías de Buenos Aires. La presencia de los patotipos se analizó mediante PCR de los genes (a) eae que codifica la proteína intimina,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AEEC; Escherichia coli; Carne molida; Calidad; Inocuidad.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200005

POPULATION ANALYSIS OF SUSCEPTIBILITY TO PROPOLIS IN REFERENCE STRAINS OF Staphylococcus aureus AND Escherichia coli
Provedor de dados: 79
Autores: FERNANDES Jr.,A.; LOPES,C. A. M.; SFORCIN,J. M.; FUNARI,S.R.C.
Susceptibility to several ethanolic extracts of propolis (EEP) concentrations was tested with the population analysis technique in reference strains of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 35218). The results of these tests showed that all bacteria were killed by EEP concentrations approximately equal or higher (2.0% v/v- S. aureus; 10.0% v/v - E. coli) than the respective minimal inhibitory concentrations (MIC). Regarding the susceptibility to propolis, there was a homogeneity of data with the respective time kill curves showing a clear bactericidal effect during 6 to 9 h of exposition.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Population analysis; Susceptibility; Staphylococcus aureus; Escherichia coli.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200005

Heterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer)
Provedor de dados: 80
Autores: Figueiredo,J. E. F.; Kalapothakis,E.; Gomez,M. V.; Bressan,W.
Insect-pests are global problems that cause severe damage to crop plants, and their control is commonly based on chemical insecticides. However, negative effects of pesticides on the environment and human health emphasize the necessity to develop alternative methods for insect-pest control. In the present study, a gene coding for the insecticidal peptide TX4(6-1) of the Brazilian armed spider (Phoneutria nigriventer) was cloned in fusion with maltose binding protein (MBP) and expressed in Escherichia coli. The affinity purified protein MBP-GlyTX4 was cleaved with the Xa factor and used for a bioassay against Spodoptera frugiperda and rabbit immunization. Five micrograms GlyTX4 protein injected into the hemocoel of larvae and abdominal cavity of adults...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinsecticide; Molecular cloning; Heterologous expression; Escherichia coli; Spodoptera frugiperda.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200006

Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water
Provedor de dados: 80
Autores: Ribeiro,DA; Niemann,FS; Gatti,MSV; Lanna,MCS; Tsuji,T; Yano,T.
Enteric infections caused by the ingestion of contaminated water, especially by Escherichia coli, are important to define the virulence properties of these bacteria. Due to frequent infantile diarrhea in the city of Ouro Preto, Minas Gerais state, Brazil, the phenotypic and genotypic diarrheagenic properties of E. coli isolated from drinking water were studied. The culture supernatants of 39 (40%) among a total of 97 E. coli isolates from drinking water were positive by suckling mouse assay and induced cytotoxic effects on Vero cells. The enterotoxic and cytotoxic activities were present in the fraction with less than 10 kDa and were not lost when heated up to 60°C and 100°C for 30 minutes. PCR assays showed that among these 39 Vero cytotoxigenic E. coli,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Drinking water; Cytotoxin; Enterotoxin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100014

Antimicrobial activity of propolis and essential oils and synergism between these natural products
Provedor de dados: 80
Autores: Probst,IS; Sforcin,JM; VLM,Rall; Fernandes,AAH; Fernandes Júnior,A.
In the present study, Apis mellifera propolis and essential oils (EOs) obtained from aromatic plants were evaluated as alternative antimicrobials. We aimed to establish the antimicrobial activity of ethanolic extracts of propolis (EEP) from Apis mellifera and of EOs from Caryophyllus aromaticus, Zingiber officinale, Cinnamomum zeylanicum and Mentha piperita against 32 Staphylococcus aureus and Escherichia coli strains from human clinical specimens. The antimicrobials were diluted in agar and the minimal inhibitory concentrations (MIC) were found whereas MIC90% values were calculated. Time-kill curve assays using mixtures containing one quarter of MIC90% for EEP with all EOs as well cinnamon EO were performed. The cinnamon EO was found to be the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Aromatic plants; Ethanolic extract of propolis; Staphylococcus aureus; Escherichia coli.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200006

A new sterile technique effective on capturing tramp ants for microbiological investigations
Provedor de dados: 82
Autores: Schuller,Lucia; Matté,Glavur R; Matté,Maria H.
Tramp ants are an outstanding group of organisms that have a definitive and important role in carrying pathogens. The purpose of this study was to develop a microbiological sterile technique of collecting ants from contaminated areas. The traps were composed of Petri dishes containing culture media, and were tested to verify the applicability of the system. A positive correlation between the microorganism growth and the presence of ants inside or around traps was observed. The technique described demonstrated to be useful to collect ants from different environments, helping the surveillance of pathogenic microorganisms that are of public health concern.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Public health; Pathogenic microorganism; Staphylococcus aureus; Klebsiella; Escherichia coli; Enterobacter.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000400023

腹膜炎を伴う巨大な片側性水賢症を発症したホルスタイン種乳牛の1症例
Provedor de dados: 19
Autores: 藤沢, 哲郎; 高橋, 英二; 山村, 拓; 古岡, 秀文; 猪熊, 壽.
後産停滞で治療中の4歳5カ月齢のホルスタイン種乳牛雌で、直腸検査により大型腫瘤を触知した。超音波検査では大型腫瘤の内部に液体および線維素様構造物を確認し、穿刺により細胞と細菌を含む淡黄色混濁液が回収された。病理解剖により腫瘤は、高度に腫大（63×37×30cm、約40kg）した左腎であり、腎盂相当部位の著しい拡張と腎実質の菲薄化がみられたことより水腎症と診断した。左腎周囲は結合組織で覆われ、腹壁および腸管との癒着がみられた。さらに腹腔内には淡黄色の腹水が貯留し、第一胃、腹壁、横隔膜、大網は広範囲に癒着しており、腹膜炎と診断された。病理組織学的検索では、左腎の正常構造の消失と重度の線維化および細菌の集簇がみられた。細菌検査では、左腎臓よりEscherichia coli およびStreptococcus sp.が分離された。水腎症の一次的原因は明らかにできなかったが、何らかの原因で片側性水腎症が形成され、細菌感染が二次的に生じ、さらに拡張した腎臓の一部が破裂して内容物が腹腔に漏れ、腹膜炎が生じたと考えられた。
Palavras-chave: 片側性水腎症; 腹膜炎; Escherichia coli; Streptococcus sp.; ホルスタイン種乳牛.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4393

Pneumonia as the cause of death in gilts experimentally infected with Trypanosoma brucei
Provedor de dados: 19
Autores: Allam, L; Ogwu, D; Agbede, R.I.S; Sackey, A.K.B.
A study was conducted in 24 gilts to determine the effect of Trypanosoma brucei infection on their reproductive efficiency. The infected gilts developed clinical trypanosomosis following a prepatent period of 2-3 days with 1.8 x 10⁶ trypanosomes per gilt. The clinical signs were observed intermittent fever, pale mucus membranes, short moist cough, moist rales, mucopurulent ocular discharges and hyperemia of the skin, reduced feed intake, and loss of body condition, recumbency, uncoordinated movements, posterior paresis and death of gilts. The cause of death in the pigs was pneumonia caused by Escherichia coli. Grossly, the lungs were severely congested and had undergone gray hepatization. Histopathologically, the lungs had thickened and congested alveolar...
Palavras-chave: Trypanosoma brucei; Immunosuppression; Escherichia coli; Pneumonia; Gilts.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3538

Sanitary aspects and technological challenges of whole milk microfiltration at low temperatures
Provedor de dados: 86
Autores: Fagnani,Rafael; Mexia,Marisa Marroni; Puppio,Ana Amélia Nunes; Battaglini,Ana Paula Pavão.
Abstract: The objective of this work was to evaluate the effects of whole milk microfiltration at low temperatures on bacterial counts and on its shelf life. The microfiltration process was evaluated at two temperatures (30 and 50ºC) and compared with the slow pasteurization process. Both slow pasteurization and microfiltration reduced the initial counts of aerobic mesophilic and psychrotrophic bacteria, as well as total coliforms in whole milk. Microfiltration at 50ºC was as effective as the pasteurization process, since it reduced the initial count of aerobic mesophilic bacteria in 4.4 log cycles; increased the product's shelf life, which reached 30 days without exceeding 1,000 CFU mL-1; and eliminated coliform counts at the temperatures of 35 and 45ºC,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Membrane; Microbiological quality; Pasteurization; Shelf life.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000800990

Clonagem e purificação de fragmento da proteína capsidial de Banana streak OL virus
Provedor de dados: 86
Autores: Lombardi,Ricardo; Harakava,Ricardo; Colariccio,Addolorata.
O objetivo deste trabalho foi clonar e induzir a expressão de fragmento da proteína capsidial de Banana streak OL virus (BSOLV-CP) em Escherichia coli, bem como purificar a proteína recombinante obtida. Empregou-se um par de iniciadores específicos para amplificar, em PCR, um fragmento de aproximadamente 390 pb, da região codificadora da porção central da BSOLV-CP. O fragmento obtido foi clonado em vetor pGEM-T Easy, subclonado em vetor pQE-30 e transformado em células de E. coli M15 (pREP4) por choque térmico. A expressão da proteína foi induzida por tiogalactopiranosídeo de isopropila (IPTG), e a proteína recombinante BSOLV-rcCP de 14 kDa foi detectada em Western blot e Dot blot. A expressão da proteína BSOLV-rcCP abre novas possibilidades para a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Badnavirus; Escherichia coli; Musa; Antissoro; Bananeira; Expressão de proteínas recombinantes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000800006

Antimicrobial effect of turmeric (Curcuma longa) on chicken breast meat contamination
Provedor de dados: 89
Autores: Lourenço,TC; Mendonça,EP; Nalevaiko,PC; Melo,RT; Silva,PL; Rossi,DA.
The aim of this study was to evaluate the efficacy of turmeric (Curcuma longa), also known in Brazil as saffron, on the reduction of Staphylococcus aureus and Escherichia coli counts in chicken meat. Forty breast meat samples were divided in two groups (A and B). In group A, 10³-10(4)E. coli (ATCC 25922) cells were inoculated and group B samples were inoculated with 10(4)-10(5)S. aureus (ATCC 9801) cells, after which each group was divided in three samples. The first sample was analyzed immediately after inoculation. The second sample (control group) was stored at 4 ºC for 48 hours and turmeric at 1% (w/w) was added to the third sample, which was homogenized and then stored under the same conditions as the second sample. E. coli and S. aureus were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turmeric; Bacterial growth; Escherichia coli; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000200002

Suppresses of Astragalus Polysaccharide on E. coli-Induced Injured Intestinal Microvascular through TLR4-NF-κB Signal Pathways in Chickens
Provedor de dados: 89
Autores: Su,L; Wang,J; Huang,J; Zhao,Y; Jiang,H; Li,H.
ABSTRACT To investigate the hypothesis that APS can attenuate E. coli-induced microvascular dysfunction in chicken intestine, 60 0-day old male chickens were divided into three groups with 5 replications of 4 chicks. Chicken in the APS group were treated with 15 mg APS daily while the others were given 0 mg APS for 6 days. Then all 7-day old chicken were injected intraperitoneally by E. coli, except for the chicken in the control group. After 4 h, all chicken’s intestinal samples were collected to detect gene expressions involved in inflammatory factors and adhesion molecules. Results showed that APS attenuated the signs of edema and hemorrhage in 7-day old chicken intestinal mucosa which were induced by E. coli injection. Consistently, APS significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astragalus polysaccharide; Escherichia coli; Intercellular adhesion molecule -1; Nuclear factor - κB; Vascular cell adhesion molecule -1.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300302

Patogenicidade In Vivo e In Vitro de Amostras de Escherichia Coli de Origem Aviária
Provedor de dados: 89
Autores: Assis,ACB; Santos,BM.
O objetivo desse trabalho consistiu em determinar a patogenicidade in vivo e in vitro de 25 amostras de Escherichia coli obtidas de aves com Síndrome da Cabeça Inchada (SCI). A patogenicidade in vivo foi determinada pela inoculação em pintos SPF de um dia de idade, utilizando-se quatro vias de inoculação: intracardíaca, saco aéreo, oral e traqueal. As aves foram avaliadas quanto à mortalidade a cada 12 horas, por um período de sete dias. Os resultados desse estudo demonstraram que as 25 amostras de E. coli, isoladas de aves com SCI, causaram mortalidade mais elevada em pintos de um dia de idade quando foram inoculadas pelas vias intracardíaca (73,6%) e saco aéreo (68%). As menores taxas de mortalidade em pintos de um dia de idade foram obtidas pelas vias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Colibacilose; Pintos; Síndrome da cabeça inchada; Verotoxina.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000200007

Uso de antibiótico, de probiótico e de homeopatia em frangos de corte criados em ambiente de conforto, inoculados ou não com Escherichia coli
Provedor de dados: 96
Autores: Boratto,Adriano José; Lopes,Darci Clementino; Oliveira,Rita Flávia Miranda de; Albino,Luiz Fernando Teixeira; Sá,Luciano Moraes; Oliveira,Gisele Andrade de.
Para avaliar o efeito do uso de antibiótico, de probiótico e de homeopatia em frangos de corte, inoculados ou não com Escherichia coli, foram distribuídos 672 frangos machos, Avian Farm, em delineamento inteiramente casualizado em esquema fatorial com oito tratamentos (quatro aditivos x com ou sem inoculação) e seis repetições, tendo 14, 12 e 7 aves por unidade experimental, respectivamente, de acordo com os períodos 1 a 11, 12 a 21 e 22 a 42 dias de idade. As aves foram alojadas em salas climatizadas em ambiente de conforto, de acordo com o manual da linhagem. Os tratamentos foram constituídos de: controle negativo (sem aditivo), antibiótico (virginiamicina + nitrovin), probiótico (meio de cultura de Lactobacilus acidophillus, Enterococcus faecium e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ambiente; Escherichia coli; Frango de corte; Promotor de crescimento.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000600014

Uso de gemas de ovos de aves hiperimunizadas contra Escherichia coli suína no controle da diarréia neonatal de leitões
Provedor de dados: 96
Autores: Ribeiro,Andréa Machado Leal; Rudnik,Liliane; Canal,Cláudio Wageck; Kratz,Lilian Ribeiro; Farias,Carolina.
Foi avaliado o efeito das gemas de ovos de aves hiperimunizadas contra Escherichia coli (E. coli) patogênica para suínos sobre a imunidade passiva de leitões recém-nascidos em uma unidade produtora de leitões. Foram avaliados a densidade ótica do ELISA (DO) para anticorpos contra E. coli, o peso corporal e a ocorrência de diarréia diária em 137 leitões recém-nascidos oriundos de 25 fêmeas primíparas, não vacinadas contra E. coli. As porcas foram consideradas blocos. De cada fêmea, foram separados seis leitões recém-nascidos de ambos os sexos, excluindo-se os mais leves e os mais pesados, divididos em três tratamentos e duas repetições. Os seguintes tratamentos foram fornecidos via oral: T1: 2 mL de PBS (tratamento controle) em duas doses, a primeira ao...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarréia; Escherichia coli; Gema; Imunoglobulina Y; Imunidade passiva; Leitões.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982005000400019

Diferentes vias de administração de probiótico sobre o desempenho, o rendimento de carcaça e a população microbiana do intestino delgado de codornas de corte
Provedor de dados: 96
Autores: Otutumi,Luciana Kazue; Furlan,Antonio Claudio; Martins,Elias Nunes; Nakamura,Celso Vataru; Garcia,Elis Regina de Moraes; Loose,Priscila Ventura.
Objetivou-se avaliar duas vias de administração de probiótico sobre o desempenho, o rendimento de carcaça e a população microbiana do intestino delgado de codornas de corte. Foram utilizadas 1.050 codornas machos e fêmeas de 1 dia de idade distribuídas em delineamento inteiramente casualizado com três tratamentos (controle, probiótico na água bebida e probiótico na ração) e cinco repetições de 70 aves. A cada sete dias, foram avaliados o ganho de peso, o consumo de ração, a conversão alimentar e a viabilidade criatória. Aos 7 e aos 14 dias, 15 aves foram abatidas para determinação da contagem de Lactobacillus sp., enterobactérias e Escherichia coli do intestino delgado e, aos 35 dias, 30 aves foram abatidas para avaliação do rendimento de carcaça e de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conversão alimentar; Escherichia coli; Lactobacilos.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000100021

Actividad antibacteriana y citotoxicidad in vivo de extractos etanólicos de Bauhinia variegata L. (Fabaceae)
Provedor de dados: 98
Autores: Martínez,Maby M; Ocampo,Diana M; Galvis,Jhon H; Valencia,Andrea.
Introducción: diversas investigaciones han identificado el potencial biológico de diferentes compuestos como flavonoides, taninos, esteroides, naftoquinonas y sesquiterpenoides presentes en el género Bauhinia. Se ha reportado para la especie Bauhinia variegata la presencia de flavonoides con propiedades antidiabéticas y antivirales; sin embargo, el estudio del potencial antibacterial y citotóxico in vivo es incipiente, tanto para compuestos de flavonoides como de alcaloides. Objetivo: determinar la actividad antibacteriana y citotóxica in vivo de los extractos alcaloidales de Bauhinia variegata L. Métodos: se aplicó cromatografía de columna para la extracción y el aislamiento de los alcaloides presentes en las hojas de B. variegata. Extractos y fracciones...
Tipo: Journal article
Palavras-chave: Alcaloides; Artemia salina; Bauhinia; Citotoxicidad; Escherichia coli.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000400002

Presencia de integrones clase I en Escherichia coli aislada de productos cárnicos en plantas Tipo Inspección Federal (TIF) en el Estado de México
Provedor de dados: 108
Autores: Fuentes Arriaga,Raquel; Talavera Rojas,Martín; Vázquez Navarrete,Jesús; Soriano Vargas,Edgardo; Gutiérrez Castillo,Adriana.
Los alimentos cárnicos constituyen uno de los principales vehículos de enfermedades transmitidas por alimentos, como consecuencia de un manejo deficiente durante su procesamiento. El objetivo del presente trabajo fue determinar la frecuencia de algunos factores de resistencia antibiótica de Escherichia coli en plantas Tipo Inspección Federal (TIF) del Estado de México. Para este fin se analizaron muestras de tres plantas TIF en el Estado de México (n = 90), 10 de materia prima (carne de bovino, cerdo y pavo), 10 de producto terminado y 70 de utensilios de trabajo. Se aislaron 18 (20%) cepas de E. coli, 3 de materia prima, 2 de producto terminado y 13 de utensilios de trabajo (P &gt; 0.05). Las E. coli aisladas presentaron una frecuencia alta de resistencia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Integrones; Multirresistencia antibiótica.
Ano: 2013
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922013000100003

Resistência estafilocócia ao nacl em leite desnatado reconstituído adicionado de nisina
Provedor de dados: 195
Autores: Lima, T. S.; Vieira, P.; Cunha, A. L. F. S.; Cabrini, C. C.; Pinto, M.
Na atualidade, no Estado de Minas Gerais, Brasil, os queijos artesanais têm ocupado uma posição de destaque em função da sua importância social e econômica. A cada ano estão ganhando maior notoriedade, entrando em diversas microrregiões produtoras no circuito tradicional, tais como as microrregiões do Campo das Vertentes e a emancipação da Serra do Salitre da microrregião do Cerrado. Há alguns anos, esse queijo só podia ser comercializado dentro do Estado de Minas Gerais, mas atualmente sua presença fora de Minas Gerais é notória. Pelo fato de ser elaborado com leite cru, o queijo apresenta risco para os consumidores por ser potencial carreador de microrganismos patogênicos como o Staphylococcus aureus. A obrigatoriedade da utilização de leite cru implica...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Segurança de alimentos; Coliformes; Escherichia coli; Staphylococcus aureus.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/34986

Celulite aviária como potencial causa de condenação de carcaças em frigorífico com inspeção federal no Rio Grande do Sul
Provedor de dados: 195
Autores: Emanuel Ferreira Damasceno, Tácito; da Silva Guahyba, Adriano; Manoel Lemes de Campos, Rogério.
A celulite aviária é um processo patológico de etiologia multifatorialcaracterizado pela inflamação purulenta aguda e difusa do tecido subcutâneo,dissecando planos teciduais e envolvendo camadas musculares. Ocorre pelacontaminação bacteriana de áreas arranhadas e a inadequação do ambiente. Aumidade excessiva da cama pode causar a evolução de arranhões para celulite.O presente trabalho estudou a incidência de lesões post-mortem característicasde celulite que resultaram em condenação de carcaças de frangos abatidos noperíodo de julho e agosto de 2011 em frigorífico inspecionado pelo Serviçode Inspeção Federal (SIF) no estado do Rio Grande do Sul. A inspeção postmortem efetuada durante o abate nas linhas de inspeção A (exame interno), B (exame de vísceras), C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celulite aviária; Escherichia coli; Condenação.
Ano: 2014
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/23847

Avaliação da pesquisa de escherichia coli nas mãos dos manipuladores de alimentos nas cozinhas da 10ª região milita r e eficiência do alcol a 70% como antisséptico
Provedor de dados: 195
Autores: Campos, Erica Ferreira Maia; Monteiro, Cynthia Levi Baratta; Oliveira, Lorena Mayana Baserra de; Figueiredo, Evania Altina Teixeira de.
O objetivos deste trabalho foi verificar o nível de contaminação por Escherichia coli das mãos dos manipuladores de alimentos das cozinhas das Organizações Militares da 10a Região Militar, sediadas em Fortaleza, além de verificar a eficiência do uso de álcool 70% como antisséptico e avaliar a importância do processo de higienização das mãos. Para tanto, foram selecionados sete manipuladores de alimentos de cada Organização Militar. Realizou-se duas coletas (antes e após a higienização) com swab estéril, padronizando-se a mão direita para amostragem. Após a primeira coleta o manipulador higienizou as mãos com sabão neutro, secou com papel toalha e aplicou álcool a 70% , aguardando a secagem natural das mãos para realização da segunda coleta. Os swabs foram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Álcool 70%; Lavagem das mãos; Antisséptico.
Ano: 2013
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/18419

Avaliação microbiológica da água em assentamentos da reforma agrária no município de Candiota, estado do Rio Grande do Sul, Brasil
Provedor de dados: 195
Autores: Müller, A.; Rockembach, C. S.; Souza, C. N.; Girolometto, G.; Schuch, L. F. D.; Pinto, F. R.
A água pode transmitir diversos contaminantes químicos e/ou biológicos; na área rural, a água de usos múltiplos pode apresentar contaminação devido à captação e ao armazenamento inadequados, bem como à falta de tratamento. O presente trabalho analisou a qualidade microbiológica da água em quatro assentamentos da reforma agrária localizados no município de Candiota, Estado do Rio Grande do Sul. Amostras de água foram coletadas em dez propriedades em quatro assentamentos, das quais 11 amostras eram utilizadas para consumo humano e seis para obtenção do leite. Foram realizadas a determinação do número mais provável de coliformes totais e de Escherichia coli, pelo método do substrato enzimático cromogênico fluorescente, e a contagem de microrganismos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coliformes; Consumo humano; Escherichia coli; Mesófilos; Ordenha.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/34940

Análise microbiológica do queijo minas artesanal do Serro-MG, Brasil, produzido com e sem fermento natural
Provedor de dados: 195
Autores: Cunha, A. L. F. S.; Paiva, V. N.; Cabrini, C. C.; Vieira, P.; Pinto, M. S.
Os queijos Minas artesanais possuem grande importância econômica, social, ambiental e política para o estado de Minas Gerais, sendo responsáveis pelo sustento direto e indireto de mais de 35 mil famílias. Até o presente momento não existem estudos envolvendo a utilização do fermento natural na fabricação de queijos Minas artesanais. Sabe-se apenas que o mesmo é utilizado de forma indiscriminada em algumas regiões produtoras tradicionais onde alguns produtores utilizam poucos mililitros para cada 100 litros de leite enquanto outros utilizam até um litro para os mesmos 100 litros. Além disso, segundo os produtores, as perdas de produção são atribuídas à qualidade desse fermento natural. O presente trabalho avaliou o efeito da adição de fermento natural sobre...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Segurança Alimentar; Coliformes; Escherichia coli; Staphylococcus aureus.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/34810

Análise da qualidade higiênico-sanitária na linha de produção de queijo muçarela produzido em laticínio do Distrito Federal, Brasil
Provedor de dados: 195
Autores: Bueno, L. S.; Ribeiro, J. L.; Poggiani, S. S. C.; Fernandes, M. L.; Souza, S. O.
Os queijos devem ser produzidos com matéria-prima de boa qualidade e submetidos a um eficiente controle em todas as etapas de processamento. A fabricação do queijo envolve vários processos, como pasteurização do leite, coagulação, corte do coágulo, dessoragem, filagem, enformagem, salga, maturação e embalagem. A manipulação do produto, o qual entra em contato com diferentes equipamentos e superfícies, aumenta o risco de contaminação microbiológica. O presente trabalho foi delineado para avaliar a qualidade higiênico-sanitária do processo de fabricação da muçarela produzida em um laticínio da região do Distrito Federal, Brasil, quanto à presença de coliformes totais e termotolerantes. Foram obtidas 15 amostras inerentes às etapas de fabricação: leite cru,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coliformes totais; Coliformes termotolerantes; Contaminação; Escherichia coli.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/35025

Spontaneous kanamycin-resistant Escherichia coli mutant with altered periplasmic oligopeptide permease protein (OppA) and impermeability to aminoglycosides
Provedor de dados: 59
Autores: Rodriguez,Mônica B.; Costa,Sérgio O. P.
A spontaneous kanamycin-resistant Escherichia coli mutant, showing cross resistance to five other aminoglycosides and absence of the OppA protein was isolated. [3H]-dihydrostreptomycin uptake is reduced in this mutant, implying that the oligopeptide transport system is involved in accumulation of aminoglycosides, although apparently not related with aminoglycoside permeability alteration due to bacterial adaptation to osmotic changes.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aminoglycosides-resistance; Escherichia coli; OppA.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200013

ANTAGONISM AGAINST VIBRIO CHOLERAE BY BACTERIAL DIFFUSIBLE COMPOUND IN THE FECAL MICROBIOTA OF RODENTS
Provedor de dados: 59
Autores: Silva,Simone Helena da; Vieira,Enio Cardillo; Nicoli,Jacques Robert.
In an ex vivo agar plate assay, we monitored the appearance of an inhibitory halo against Vibrio cholerae from the feces of Wistar and Fischer rats aged 10 to 42 days. The frequency of Wistar rats showing halo increased from 0% (10 days) to a maximum of 80.0% (29 days) and then decreased to 53.3% (42 days). A similar pattern was obtained with Fischer rats but with a lower intensity (maximum frequency of 50.0% by day 36). In a separate experiment, when Wistar rats were fed a low-protein diet for 7 days, the inhibitory halo decreased drastically. Three apparently different colony morphologies were isolated from the dominant fecal microbiota: a facultative anaerobe (FAN) and two strict anaerobes (SAN). The ex vivo inhibitory test showed a halo around the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vibrio cholerae; Escherichia coli; Streptococcus intermedius; Antagonism; Rodents.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300016

Cepas de Lactobacillus spp. con capacidades probióticas aisladas del tracto intestinal de terneros neonatos
Provedor de dados: 101
Autores: Sánchez,Lilian; Omura,Michele; Lucas,Adams; Pérez,Tania; Llanes,Marisleidys; Ferreira,Celia de Luce.
Se caracterizaron in vitro 17 cepas de Lactobacillus spp. aisladas del tracto intestinal de terneros neonatos, para seleccionar las mejores como candidatos probióticos y realizarles posteriormente los estudios in vivo. A todos los aislados se les realizó la prueba de la catalasa, la tinción de Gram y se estudió sus características culturales para su identificación preliminar. Se evaluó su capacidad probiótica mediante pruebas in vitro como fueron el crecimiento a diferentes temperaturas (30, 37 y 45°C), pH (2,5; 3,4; 5,4; 6,4) y capacidad de acidificación del medio de cultivo. Se preseleccionaron 10 cepas de Lactobacillus spp. que crecieron en todas las condiciones evaluadas. Se destaca el crecimiento de células viables de 6,7-10 log UFC/ml que...
Tipo: Journal article
Palavras-chave: Lactobacillus spp.; Probiótico; Salmonella sp.; Staphylococcus aureus; Escherichia coli; Listeria monocytogenes.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000200004

APLICACIÓN DE TÉCNICAS MOLECULARES EN LA CARACTERIZACIÓN DE AISLADOS DE Escherichia coli PROCEDENTES DE CERDOS CON SÍNDROME DIARREICO EN LA PROVINCIA DE VILLA CLARA
Provedor de dados: 101
Autores: Lazo Pérez,L; Dahbi,Ghizlane; Blanco Álvarez,M; Blanco Álvarez,J.E; Blanco Álvarez,J; LLorens Blanco,F.
Escherichia coli es una importante causa de diarrea en lechones recién nacidos y postdestetados, y ocasiona importantes pérdidas económicas en las explotaciones porcinas en todo el mundo. En el presente trabajo se realizó una caracterización de los aislados de Escherichia coli, a partir de muestras provenientes de cerdos con síndrome diarreico en la provincia de Villa Clara, para lo cual se aplicaron diferentes métodos de diagnóstico como la detección de los genes que codifican factores de virulencia, el serotipado y el estudio de los perfiles de electroforesis en campos pulsantes (PFGE). Los resultados mostraron, que en los procesos diarreicos causados por Escherichia coli, que afectan al cerdo durante la etapa neonatal y postdestete en diferentes granjas...
Tipo: Journal article
Palavras-chave: Diarrea; Escherichia coli; Seropatotipos; Perfiles de electroforesis en campos pulsantes (PFGE).
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000200004

ISOLATION AND IDENTIFICATION OF Ornithobacteriun rhinotracheale FROM LAYING HENS IN FARMS OF LA HABANA PROVINCE
Provedor de dados: 101
Autores: Espinosa,Ivette; Colas,M; Vichi,Joivier; Báez,M; Martínez,Siomara.
Respiratory infection is the most serious disease affecting poultry and causing great economic losses to the poultry industry worldwide. In avian host, several microorganisms of the genus Pasteurella (P. multocida, P. gallinarum and P. anatipestifer), Bordetella (B. avium) and Avibacterium paragallinarum) were involved in respiratory diseases complex. Ornithobacterium rhinotracheale is a recently discovered bacterium, of worldwide distribution in commercial poultry, in which it is associated to respiratory diseases and it is also found in wild birds. Airsacculitis and pneumonia are the most common symptoms of infection with O. rhinotracheale. Isolation and identification of pathogenic bacteria associated to respiratory diseases were carried out from a...
Tipo: Journal article
Palavras-chave: Pasteurella multocida; Escherichia coli; Ornithobacterium rhinotracheale; Layer hens.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2011000100006

Efficacy of electrolyzed-oxidizing water for inactivating Escherichia coli inoculated on holy basil
Provedor de dados: 106
Autores: Jantima Japakaset; Siriporn Stonsaovapak; Pornthip Charoenthamawat; Wanchai Panthavee.
Tipo: PhysicalObject
Palavras-chave: Electrolyzed-oxidizing water; Holy basil; Antimicrobial treatments; Escherichia coli; Food sanitation; น้ำอิเล็กโทรไลซ์; เชื้อจุลินทรีย์; การฆ่าเชื้อ; กะเพรา; เชื้ออีโคไล; ความปลอดภัยของอาหาร.
Ano: 2006
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/2638

Evaluación de la tasa de aclaración de la almeja dulceacuícola Anodontites tortolis en un medio con coliformes Escherichia coli
Provedor de dados: 109
Autores: Mallama Leyton,Juan René; Guzmán Alvis,Angela Inés.
En condiciones de laboratorio se evaluón acute; la eficiencia de la almeja de agua dulce (Anodontites tortolis) para disminuir la carga de coliformes fecales (Escherichia coli) determinada mediante filtración en membrana por 8 horas. El experimento se diseñó con dos tratamientos (densidades de seis (T1) y ocho almejas (T2) más un control) con tres replicaciones. Se encontraron diferencias significativas (P&lt;0.05) en las tasas de aclaración entre tratamientos. Los mayores niveles de remoción de E. coli se presentan en el tratamiento con mayor cantidad de individuos y la tasa de aclaración varió entre 0,47 y 3,80 l/h por individuo. La almeja mostró tasas de aclaración dentro de los rangos registrados en la literatura, lo que hace a este organismo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Almeja; Anodontites tortolis; Biofiltros; Bivalvos; Coliformes; Escherichia coli; Tasa de aclaración.
Ano: 2011
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122011000100007

AVALIAÇÃO DA ATIVIDADE ANTIBACTERIANA E MODULADORA DE AMINOGLICOSÍDEOS DO ÓLEO ESSENCIAL DE Cymbopogon citratus (DC.) Stapf
Provedor de dados: 37
Autores: F. LUCENA,Bruno F; TINTINO,Saulo R; FIGUEREDO,Fernando G; DE M. OLIVEIRA,Cícera Datiane; DOS S. AGUIAR,José J; DO N. CARDOSO,Edmilson; A. DE AQUINO,Pedro E; ANDRADE,Jacqueline C; M. COUTINHO,Henrique D; F. MATIAS,Ednardo F.
Vários trabalhos vêm demonstrando a importância do estudo de produtos naturais como fonte alternativa para novos antimicrobianos ou que venham potencializar os já existentes. Neste contexto este trabalho teve como objetivo investigar a atividade antibacteriana e as possíveis interações entre o óleo essencial de Cymbopogon citratus combinados a aminoglicosídeos frente a linhagens padrões e multirresistentes de S. aureus, E. coli e de P. aeruginosa provenientes de isolados clínicos. Um ensaio de microdiluição foi realizado para verificar a atividade antibacteriana e as possíveis interacções entre o produto natural e os antibióticos, utilizando uma concentração sub-inibitória. Através dos resultados foi constatado a interferência sinérgica dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cymbopogon citratus; Escherichia coli; Óleo essencial; Pseudomonas aeruginosa; Staphylococcus aureus.
Ano: 2015
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100005

Antibacterial activity of Aroeira honeys produced in Minas-Gerais against bacteria of clinical importance
Provedor de dados: 3
Autores: Viana, Fabiana Ribeiro; Carmo, Luiz Simeão do; Bastos, Esther Margarida Alves Ferreira.
The biological activity of honey has been the subject of great scientific investigation. Bee products are widely used in folk medicine to treat human diseases due to their antibacterial and inhibitory potential action on different human pathogens. Ten honey samples produced by Apis mellifera in the northern of Minas Gerais state were tested against pathogenic microorganisms Staphylococcus aureus TSST (clinical isolated) and enterohaemorrhagic Escherichia coli ATCC 43895 for determination of their minimum inhibitory concentrations. The microdilution technique in broth Mueller-Hinton broth (MHB) was used in four concentrations (25, 12.5, 6.25 and 3.125%). There was a reduction of bacterial growth for the two target species at all concentrations tested. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bee products; Antibacterial activity; Staphylococcus aureus; Escherichia coli.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36766

Detección del crecimiento de Escherichia Coli con termografía infrarroja
Provedor de dados: 115
Autores: Hernández-Eugenio,Guadalupe; Echeverría-Landín,Eliubi; Hahn Schlam,Federico.
Resumen: La determinación de contaminación microbiológica debe ser rápida y se debiera medir in-situ. En el presente trabajo se desarrolló una metodología rápida y portátil para la detección de Escherichia coli, basada en el análisis de imágenes térmicas de infrarrojo. Cultivos de E. coli en medio clásico agar LEVINE fueron fotografiados en imágenes térmicas, obtenidas mediante una cámara infrarroja (IR). Dos prototipos fueron acoplados a la cámara IR con la finalidad de mantener una temperatura constante (37 °C) de las muestras. El primero de ellos radiaba calor en la base de las muestras mientras el segundo calentaba la muestra radialmente. La cinética de crecimiento fue seguida con imágenes térmicas, encontrando que el tiempo mínimo de detección de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Cámara infrarroja; Emisividad; Radiómetro.
Ano: 2007
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952007000500503

Prevalence of virulence factors in Escherichia coli strains isolated from the genital tract of healthy cows
Provedor de dados: 45
Autores: Resende,D.; Santo,E.; Macedo,C.; Marin,J.M.
The prevalence of virulence genes expressing fimbriae, production of hemolysin, colicin and aerobactin, was determined in Escherichia coli isolates from healthy cow’s genital tract not showing clinical signs of infection. The presence of fimbriae expression genes (pap, sfa, afa) was assayed using specific primers in a polymerase chain reaction; none were detected in any of the isolates. Yet, a prevalence of 90.4%, 69.8%, and 28.5% of virulence factors for colicin, hemolysin and aerobactin respectively, was detected in the isolates. Analysis of the bacterial pathogenicity of isolates from the bovine genital tract may contribute towards the understanding of E. coli behavior.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cow; Escherichia coli; Genital tract; Virulence factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300003

Caracterização molecular e fenotípica de estirpes de Escherichia coli produtoras de shiga-toxina (STEC) não-O157 de fezes e carcaças bovinas
Provedor de dados: 45
Autores: Carvalho,A.F.; Miyashiro,S.; Nassar,A.F.C.; Noda,A.; Gabriel,D.T.; Baldassi,L.
Foram coletados 100 suabes retais e 100 suabes de carcaças bovinas em matadouros do estado de São Paulo, e um total de 326 estirpes de E. coli foram identificadas, sendo 163 de amostras retais e 163 de amostras de carcaça. Todos os isolados submetidos à PCR para detecção dos genes das toxinas Stx1 e Stx2 foram identificados como não-O157 e fenotipados pelo teste da citotoxicidade em células Vero. Das 26 estirpes que apresentaram apenas o gene stx1, das 56 que apresentaram apenas o gene stx2 e das 30 estirpes que apresentaram ambos os genes, 17 (65,4%), 42 (75%) e 22 (73,3%), respectivamente, foram positivas ao teste de citotoxicidade. Não houve diferença estatística entre os três perfis genéticos e na positividade ao teste de citotoxicidade. Os resultados...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Escherichia coli; STEC; Fezes; Carcaça.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400014

Piometra em uma leoa (Panthera leo): relato de caso
Provedor de dados: 45
Autores: Murer,L.; Ribeiro,M.B.; Machado,L.; Sagave,L.; Kommers,G.D.; Galiza,G.J.N.; Moreira,N.; Lovato,M.
A piometra é uma infecção aguda ou crônica do útero que ocorre frequentemente em cadelas não castradas, podendo também ocorrer em gatas domésticas e selvagens, sendo poucos os estudos relacionados à piometra em grandes felídeos. O objetivo deste relato foi descrever um caso de piometra em uma leoa (Panthera leo) de cativeiro, as lesões de necropsia e histológicas, bem como os resultados da análise microbiológica. Uma leoa com aproximadamente 23 anos, pertencente a um criadouro conservacionista de Santa Maria-RS, foi encontrada morta pela manhã em seu recinto. Após coleta de dados, procedeu-se à necropsia e à coleta de material para análise histopatológica e bacteriológica. A análise microbiológica revelou predomínio das bactérias Streptococcus sp. e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infecção uterina; Grandes felídeos; Streptococcus sp.; Escherichia coli; Septicemia.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300727

Resistência a antimicrobianos de Escherichia coli isolada de dejetos suínos em esterqueiras
Provedor de dados: 45
Autores: Silva,F.F.P.; Santos,M.A.A.; Schmidt,V.
The antimicrobial resistance of 96 Escherichia coli strains isolated from a stabilization pond system on a pig-breeding farm was evaluated. Strains were tested for their resistance against 14 antimicrobial using the agar diffusion method. E. coli strains showed resistance to tetracycline (82.3%), nalidixic acid (64%), ampicilin (41%), sulfamethoxazole/trimethoprin (36%), sulfonamide (34%), cloranphenicol (274%), ciprofloxacin (19%), cefaclor (16%), streptomicyn (7.3%), neomicyn (1%), amoxacilin/ clavulanic acid (1%), and amikacin (1%). No resistance was observed to gentamicin and tobramycin, and 37.5% of E. coli strains were resistant to four or more antimicrobials. The multiresistance pattern was found in strains isolated during all sampled period....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Resistência antimicrobiana; Dejetos suínos; Esterqueira.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300035

Genetic heterogeneity of Escherichia coli strains isolated from raw milk, Minas Frescal cheese, and food handlers
Provedor de dados: 45
Autores: Campos,M.R.H.; André,M.C.D.P.B.; Borges,L.J.; Kipnis,A.; Pimenta,F.C.; Serafini,A.B.
From February 2004 to March 2005, 140 samples of food handlers - hands and nostrils - (92), raw milk (24), and minas frescal cheese (24) were analyzed for the presence of Escherichia coli in a dairy processing plant of Goiás State. Forty-seven E. coli strains were obtained and compared by DNA macrorestriction patterns obtained from pulsed-field gel electrophoresis following XbaI restriction in order to investigate the possible sources of cheese contaminations. Based on PFGE genotyping, one strain isolated from food the hands of a handler and five strains isolated from raw milk were identical or closely related to six strains from cheese suggesting, in these cases, the probable source of E. coli contamination in cheeses. No strain isolated from the nostrils...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Pulsotypes; Minas frescal cheese; Raw milk; Food handlers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000500025

Serogroup identification, phenotypic detection of hemolysis and extended spectrum beta-lactamases of Escherichia coli isolated from psittacine of illegal wildlife trade in Fortaleza, Brazil
Provedor de dados: 45
Autores: Lopes,E.S.; Cardoso,W.M.; Nishi,D.M.; Horn,R.V.; Albuquerque,Á.H.; Lima,S.V.G.; Beleza,A.J.F.; Gaio,F.C.; Carmo,C.C.; Pascoal Filho,M.N.; Teixeira,R.S.C.
ABSTRACT This study aimed to identify serogroups of Escherichia coli important for human health in isolates from psittacine of illegal wildlife trade in Ceará State. In addition, hemolysis and production of Extended Spectrum Beta-Lactamases (ESBL) was assessed in the isolates. A total of 78 E. coli strains isolated from different Psittaciformes species from a wildlife rehabilitation center in Fortaleza, Brazil. The isolates used in this study were previously identified and stored. Serogroup identification was performed using polyvalent sera for EPEC (O55, O111, O119, O114, O125, O86, O126, O127, O128), EIEC (O136, O124) and EHEC (O157). ESBL detection was performed with double disk synergy method. For hemolysis detection, isolates were inoculated in blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Serogroups; ESBL; Hemolysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300823

Multirresistência antimicrobiana em cepas de Escherichia coli isoladas de cadelas com piometra
Provedor de dados: 45
Autores: Lara,V.M.; Donadeli,M.P.; Cruz,F.S.F.; Carregaro,A.B.
The antimicrobial sensibility of Escherichia coli strains isolated from the uterine content of bitches was evaluated. Fifteen E. coli strains were tested in relation to their susceptibility to different antimicrobials. The results demonstrated 100% of resistance to all tested drugs, being a quite conflicting finding compared to other works, which observed variable resistance of those bacteria to different antimicrobials but not the same multi-resistance pattern. The detection of those multi-resistance strains configures a problem, with important implications on the antimicrobial therapy. Therefore, additional investigations for a best characterization and extension of this problem are needed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cadela; Escherichia coli; Piometra; Antibiograma.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400040

Avaliação de ensaios analíticos para detecção de coliformes fecais em queijo Minas
Provedor de dados: 45
Autores: Pereira,M.L.; Gastelois,M.C.A.; Bastos,E.M.A.F.; Caiaffa,W.T.; Faleiro,E.S.C.
Foram submetidas à pesquisa de coliformes fecais, utilizando-se a técnica do número mais provável (NMP/g), 168 amostras de variedades de queijo Minas (20 frescal, 48 canastra e 100 padronizado) coletadas em Belo Horizonte. Para a comparação de diferentes ensaios em temperatura elevada, utilizou-se o caldo EC isoladamente, e caldos EC e triptofano em paralelo. Visando à pesquisa de indol foi realizado ensaio para confirmação de produção de beta-D-glucuronidase e indol em caldo fluorocult lauril sulfato. Os resultados demonstraram não haver diferença estatística significativa entre as três metodologias utilizadas para a pesquisa de coliformes fecais, considerando os índices de aceitação definidos pelos padrões legais de inspeção de queijo Minas. A facilidade...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Queijo Minas; Coliformes fecais; Escherichia coli.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500004

Fatores de virulência em linhagens de Escherichia coli isoladas de mastite bovina
Provedor de dados: 45
Autores: Ribeiro,M.G.; Costa,E.O.; Leite,D.S.; Langoni,H.; Garino Júnior,F.; Victória,C.; Listoni,F.J.P.
Avaliou-se a ocorrência de fatores de virulência e do sorotipo O157:H7 em 120 linhagens de Escherichia coli, isoladas de 80 casos de mastite clínica bovina e 40 de mastite subclínica. Verificou-se alfa-hemolisina em oito (6,7%) linhagens, isoladas de cinco casos de mastite clínica e três de mastite subclínica e em nenhuma das estirpes detectou-se enteroemolisina. A presença de sideróforos foi encontrada em 11 (9,2%) linhagens, sete de mastite clínica e quatro de subclínica. Em duas (1,7%) estirpes isoladas de mastite subclínica, identificou-se enterotoxina STa. Observou-se efeito citopático em células vero compatível com a produção de verotoxina-VT em cinco (4,2%) linhagens, duas de mastite clínica e três subclínicas. Em uma (0,8%) linhagem isolada de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Mastite; Escherichia coli; Virulência; Toxina; Sorotipo O157:H7.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500004

Virulence, resistance, and genetic relatedness of Escherichia coli and Klebsiella sp. isolated from mule foals
Provedor de dados: 45
Autores: Carneiro,V.C.; Lessa,D.A.B.; Guttmann,P.M.; Magalhaes,H.; Aquino,M.H.C.; Cunha,L.E.R.; Arais,L.R.; Cerqueira,A.M.F.
ABSTRACT Respiratory diseases are common in young horses but little is known about such infections in mule foals. This study aimed to characterize Escherichia coli and Klebsiella sp. isolated from tracheal wash (TW) and fecal samples (FS) of mule foals, with or without cytological evidence of respiratory disease. Strains were analyzed against 13 antimicrobials, for presence of Extended spectrum beta-lactamase (ESBL), and virulence genes. Phylogrouping and Randomic (RAPD)-PCR profiles were used to evaluate their genetic relatedness. E. coli strains from TW and FS showed greatest resistance to tetracycline, while Klebsiella strains were mainly resistant to ampicillin; multidrug resistance and ESBL production were also detected. The blaCTX gene prevailed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Klebsiella; Respiratory disease; Virulence; Antimicrobial resistance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501073

Detecção de cepas patogênicas pela PCR multiplex e avaliação da sensibilidade a antimicrobianos de Escherichia coli isoladas de leitões diarréicos
Provedor de dados: 45
Autores: Macêdo,N.R.; Menezes,C.P.L.; Lage,A.P.; Ristow,L.E.; Reis,A.; Guedes,R.M.C.
Avaliou-se a freqüência dos genes de fímbrias (K88, K99, 987P, F18 e F41) e toxinas (LT, Stb, StaP e Stx2e) de cepas de E. coli isoladas de leitões com diarréia usando a técnica de PCR multiplex com primers específicos para esses genes, e estudou-se o padrão de sensibilidade das cepas patogênicas pelo método de difusão em disco ao florfenicol, ceftiofur sódico, colistina, fosfomicina, neomicina, norfloxacina, sulfa + trimetoprim, doxiciclina, tetraciclina e lincomicina. Foram utilizadas 144 amostras de E.coli isoladas de leitões com diarréia, provenientes de granjas localizadas no estado de Minas Gerais. Dessas, 42 (29,2%) foram positivas para pelo menos um dos fatores de virulência testados. Dentre essas 42 amostras, 23 (54,8%) apresentaram genes de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Escherichia coli; Tipificação; PCR multiplex; Sensibilidade antimicrobiana.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500005

Virulence factors of Escherichia coli isolated from diarrheic calves
Provedor de dados: 45
Autores: Rigobelo,E.C.; Gamez,H.J.; Marin,J.M.; Macedo,C.; Ambrosin,J.A.; Ávila,F.A.
One hundred seventy-three Escherichia coli strains isolated from calves from northwestern São Paulo State, having diarrhea were examined for the production of thermolabile (LT) and thermostable (ST) enterotoxins and for the presence of virulence factors associated with bovine colibacillosis. Eighty-five (49.1%) of the E.coli strains produced toxins; 53 isolates were detected as producing STa toxin, and 9 also produced LT toxin. By PCR, 23 isolates were shown to harbor only the LT-II gene. Nine (5.2%) isolates harbored Shiga toxin genes: four carried the stx2 gene, four the stx1 gene and one carried both. Three of the isolates showing stx1 also carried the eae gene. Among the E. coli isolates examined for susceptibility to 10 antimicrobial agents,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calf; Diarrhea; Escherichia coli; Virulence factor; Antimicrobial agent.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300003

Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil
Provedor de dados: 45
Autores: Paneto,B.R.; Schocken-Iturrino,R.P.; Macedo,C.; Santo,E.; Marin,J.M.
The occurrence of toxigenic Escherichia coli in raw milk cheese was surveyed in Middle Western Brazil. Fifty samples of cheese from different supermarkets were analyzed for E.coli. The isolates were serotyped and screened for the presence of verotoxigenic E. coli (VTEC) and enterotoxigenic E. coli (ETEC) by Polymerase Chain Reaction (PCR). The susceptibility to thirteen antimicrobial agents was evaluated by the disk diffusion method. E.coli were recovered from 48 (96.0%) of the samples. The serogroups identified were O125 (6.0%), O111 (4.0%), O55 (2.0%) and O119 (2.0%). Three (6.0%) and 1(2.0%) of the E.coli isolates were VTEC and ETEC, respectively. Most frequent resistance was observed to the following antimicrobials: cephalothin (60.0%), nalidixic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Cheese; VTEC; ETEC; Antimicrobial resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200035

Bactérias zoonóticas isoladas de Passeriformes silvestres recuperados do tráfico de animais no estado do Ceará/Brasil
Provedor de dados: 45
Autores: Gaio,F.C.; Lopes,E.S.; Lima,B.P.; Carmo,C.C.; Marques,A.R.; Oliveira,F.R.; Amaral,M.S.M.G.; Pascoal Filho,N.M.; Carreira,A.S.; Beleza,A.J.F.; Teixeira,R.S.C.; Havt,A.; Maciel,W.C.
RESUMO A ordem dos Passeriformes é uma das mais pressionadas pelas ações antrópicas, especialmente as relativas ao tráfico de animais, que, devido às más condições de manejo e higiênico-sanitárias, favorecem a infecção dos espécimes por patógenos virulentos e zoonóticos, como cepas de Escherichia coli e Salmonella spp., cujo isolamento em suabes cloacais, bem como a análise dos genes de virulência das cepas de E. coli foram objetivos do estudo. Para isso, 120 Passeriformes silvestres nativos, recebidos pelo Cetas/CE, foram avaliados individualmente. As cepas isoladas foram submetidas a teste de disco difusão para determinação da sensibilidade aos antimicrobianos. Em etapa posterior, foi realizada PCR para a detecção de oito genes de virulência dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passeriformes silvestres; Tráfico de animais; Zoonoses; Escherichia coli; Saúde pública.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501488

Colimetria de água marinha em áreas de cultivo e extrativismo de mexilhões no município de Niterói, RJ
Provedor de dados: 45
Autores: Pinheiro Jr.,A.A.; Oliveira,L.A.T.; Franco,R.M.; Carvalho,J.C.A.P.
Analisaram-se amostras de água do mar de cinco pontos da Baía de Guanabara quanto ao número mais provável (NMP) de coliformes totais e de Escherichia coli usando-se o método fluorogênico, e verificaram-se o pH, a salinidade e a temperatura dos locais de colheita. As contagens de E. coli nas amostras das estações Icaraí, Boa Viagem e Santa Cruz foram, em média, acima do previsto na legislação, enquanto que nas estações Piratininga e Rio Branco, 92,9% e 100% das amostras tiveram contagens de E. coli menor que 3/100ml. Observou-se relação positiva entre coliformes totais e E. coli, enquanto que a correlação entre colimetria, pH, salinidade e temperatura da água não foi significativa. Temperatura e pH mostraram pouca variação entre as estações, sendo as médias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coliformes; Escherichia coli; Águas de cultivo; Baía de Guanabara.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000400016

Transference in vitro of the resistance to the antimicrobials between Escherichia coli, Lactobacillus spp. and Salmonella enteritidis isolated from chickens
Provedor de dados: 45
Autores: Okamoto,A.S; Andreatti Filho,R.L; Rocha,T.S; Milbradt,E.L.
The objective of this work was to verify the possibility of transference of resistance to the antimicrobials between bacteria that are in the present normal microbiota of chickens and Salmonella Enteritidis. Samples of Lactobacillus spp. (L. spp.), Salmonella Enteritidis (SE) and Escherichia coli (E. coli) previously isolated from chickens, selected after the test of sensitivity antimicrobial in vitro according the standard method (National Committee for Clinical Laboratory Standards) utilizing those with resistance and sensibility to the antimicrobials inductors, named donor and receptor bacteria, respectively were used. Antimicrobials inductors were utilized to stimulate the transference of resistance to the antimicrobials between the bacteria. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transfer; Resistance to antimicrobials; Escherichia coli; Salmonella Enteritidis; Lactobacillus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500015

Fator necrosante citotóxico em Escherichia coli isolada de mastite clínica bovina
Provedor de dados: 45
Autores: Ribeiro,M.G.; Costa,E.O.; Leite,D.S.; Ferreira,A.J.P.; Silva,A.S.; Della Colleta,H.H.M.
This report describes the production of cytotoxic necrotizing factor (CNF) by an Escherichia coli strain isolated from clinical bovine mastitis with clinical signs of toxemia The animal had hemorrhages and necrosis of the mammary glands, and died within 24 hours after the onset of clinical signs. In addition to CNF identification, alpha-haemolysin and siderophores production were also characterized in this strain. This report reinforce the association of CNF and alpha-haemolysin production in E. coli virulence associated with clinical cases of severe bovine mastitis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastite bovina; Escherichia coli; Fatores de virulência; Fator necrosante citotóxico.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000600015

Avaliação da eficiência de um probiótico no controle de diarréia e no ganho de peso de bezerros
Provedor de dados: 45
Autores: Ávila,F.A.; Paulillo,A.C.; Schocken-Iturrino,R.P.; Lucas,F.A.; Orgaz,A.; Quintana,J.L.
Foram utilizadas 99 vacas prenhes distribuídas em oito grupos que receberam os seguintes tratamentos: grupo I, com 29 vacas não vacinadas e seus bezerros que não receberam probiótico, ficando como controle; grupo II, com 10 vacas vacinadas e seus bezerros que não receberam probiótico; grupos III, IV e V, com 10 animais cada, vacas vacinadas e seus bezerros que receberam probiótico durante 5, 15 e 30 dias, respectivamente; os grupos VI, VII e VIII, com 10 animais cada, vacas não vacinadas e seus bezerros que receberam probiótico durante 5, 15 e 30 dias, respectivamente. Cada animal dos grupos vacinados recebeu duas doses vacinais contendo os pili K99 e A14 de Escherichia coli na dose de 5,0ml por via subcutânea. O probiótico contendo Ruminobacter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Escherichia coli; Probiótico; Diarréia.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000100011

Resistência a antimicrobianos dependente do sistema de efluxo multidrogas em Escherichia coli isoladas de leite mastítico
Provedor de dados: 45
Autores: Moreira,M.A.S.; Ferreira,A.B.; Trindade,T.F.S.L.; Reis,A.L.O.; Moraes,C.A.
Identificaram-se e caracterizaram-se a resistência e a multirresistência aos principais antimicrobianos usados no tratamento de mastite bovina causada por Escherichia coli. A concentração inibitória mínima (MIC) e o sistema de efluxo foram detectados pelas curvas de crescimento, com base na densidade óptica, em diferentes concentrações da droga e na presença e na ausência do desacoplador da força próton-motora (PMF). E. coli 1 foi resistente à neomicina e à gentamicina; E. coli 3 e 4, à tetraciclina e à estreptomicina; e E. coli 2 e 6 à gentamicina. E. coli 5 apresentou modelo de sensibilidade. Observou-se que MICs de todos os antimicrobianos dos multirresistentes (E. coli 1, 3 e 4) diminuíram na presença do desacoplador, o que sugere sistema de efluxo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Sistema de efluxo; Multirresistência; Mastite; Antimicrobiano.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600003

Experimental peritonitis in horses: peritoneal fluid composition
Provedor de dados: 45
Autores: Mendes,L.C.N.; Marques,L.C.; Bechara,G.H.; Peiró,J.R.
Sixteen adult horses were randomly divided into four equal groups of four animals and each group was injected intraperitoneally with one of the following suspension: Group I, 100×10(7) colony-forming units (CFU) of E. coli diluted in 500ml of 0.9% saline; Group II, 100×10(7) CFU of Bacteroides fragilis in 500ml of 0.9% saline; Group III, 100×10(7) CFU of E. coli in combination with 100×10(7) CFU of B. fragilis in 500ml of 0.9% saline; Group IV, 500ml of 0.9% saline. A significant increase in leukocyte number was observed in the peritoneal fluid by four hours after the inoculations in animals of Group I and II, and by eight hours in animals of Group III. The highest cell count observed was 516×10³ leukocytes/mm³. Significant increases in peritoneal fluid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horses; Peritonitis; Escherichia coli; Bacteroides fragilis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000300003

Caracterização genotípica dos isolados de Escherichia coli provenientes de frangos de corte
Provedor de dados: 45
Autores: Silva,I.M.M.; Evêncio-Neto,J.; Silva,R.M.; Lucena-Silva,N.; Magalhães,J.; Baliza,M.
Caracterizaram-se genotipicamente os isolados de Escherichia coli oriundos de fígado de frangos provenientes de dois matadouros avícolas. Foram coletadas 62 amostras de fígados de frangos, sendo 30 macroscopicamente inalterados e 32 com alteração macroscópica e que originaram no descarte da carcaça. Isolaram-se 30 cepas de Escherichia coli pelo método clássico, sendo 21 isoladas de fígados inalterados e nove provenientes de carcaças rejeitadas. Utilizou-se a reação em cadeia de polimerase para verificação de genes de virulência de E. coli, incluindo o gene de resistência sérica (iss) para identificação de E. coli patogênica para aves, o gene para Shiga cytotoxin 1 e 2 (stx) para identificação de E. coli enteroemorrágica, o gene bfpA para identificação de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Frango; Escherichia coli; Caracterização genética.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000200010

Anti-Escherichia coli activity of Brazilian plant extracts: New trends in Veterinary research
Provedor de dados: 45
Autores: Camargo,L.R.P.; Suffredini,I.B.
Escherichia coli é uma bactéria presente no trato intestinal de animais de sangue quente. Entretanto, a perda do equilíbrio em sua relação com o hospedeiro pode causar doenças que levam a perdas econômicas, quando se trata de animais de produção, e perdas incalculáveis, quando se trata de animais silvestres. O presente estudo testou diversos extratos aquosos e orgânicos, obtidos de plantas da Amazônia brasileira, contra Escherichia coli (ATCC 25922). Usando o ensaio de disco difusão em ágar Mueller-Hinton (DDA), foram testados 1.791 extratos quanto à sua atividade antibacteriana. Os extratos que mostraram halo de inibição de crescimento bacteriano foram testados no modelo da microdiluição em caldo (MDBC) contra uma suspensão bacteriana preparada na escala...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Plant extracts; Pathogenicity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200041

Genes de enterotoxinas e perfil antimicrobiano de Escherichia coli isoladas de suínos hígidos no Distrito Federal
Provedor de dados: 45
Autores: Drummond,V.O.; Perecmanis,S.
Um total de 127 cepas de Escherichia coli foi isolado de suínos no Distrito Federal, testado para a presença de genes de enterotoxinas (STa, LT-I, LT-II, Stx1 e Stx2) e para resistência antimicrobiana. Das cepas isoladas, oito (6,3%) possuíam genes para enterotoxinas, sendo quatro (3,2%) positivas somente para LT-I, três (2,4%) somente para STa e uma (0,8%) positiva para STa e LT-I. Nenhuma das cepas isoladas apresentou genes para LT-II, Stx1 ou Stx2. Quanto ao perfil de resistência antimicrobiano, os antibióticos com maiores porcentagens de resistência foram lincomicina (100%), sulfonamidas (74,8%) e tetraciclina (70,1%), enquanto os maiores índices de sensibilidade foram observados na norfloxacina (82,7%), gentamicina (75,6%) e sulfametoxazol +...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Suíno; Enterotoxinas; Resistência antimicrobiana.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400010

Indicadores físico-químicos e atividade antibacteriana de própolis marrom frente à Escherichia coli
Provedor de dados: 45
Autores: Bastos,E.M.A.F; Galbiati,C; Loureiro,E.M; Scoaris,D.O.
The activity of 23 samples of ethanolic brown propolis, from the State of Mato Grosso, was investigated against Escherichia coli ATCC 25922. The values of physical and chemical parameters showed significant variation among samples. The percentage of dry extract ranged from 2.6 to 27.6%. The index of oxidation varied from 3 to 519 seconds. All samples showed the percentage of wax higher than the limit preconized by the legislation, with values varying from 3.4 to 74.6%. The quantification of phenolic and flavonoid compounds, responsible for antimicrobial activity, ranged from 0.1 to 5.0 (w/w) and 0.02 to 0.66 (w/w), respectively, being that the higher the index of phenolic compounds the larger the zones of inhibition. Antibacterial activity was observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abelha; Própolis; Atividade antibacteriana; Indicadores físico-químicos; Escherichia coli.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500032

Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli
Provedor de dados: 47
Autores: Rüttler,M.E.; Yanzón,C.S.; Cuitiño,M.J.; Renna,N.F.; Pizarro,M.A.; Ortiz,A.M.
Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator ( aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 ( astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a multiplex PCR assay which checks aggR and astA genes was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Escherichia coli; Cell culture; PCR (Polymerase Chain Reaction).
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200006

Characterization of Escherichia coli virulence genes, pathotypes and antibiotic resistance properties in diarrheic calves in Iran
Provedor de dados: 48
Autores: Shahrani,Masoud; Dehkordi,Farhad Safarpoor; Momtaz,Hassan.
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Tipo: Journal article
Palavras-chave: Antibiotic resistance profile; Diarrheic calves; Escherichia coli; Iran; Serogroups; Virulence genes.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100028

Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride
Provedor de dados: 48
Autores: SOUZA,RAPHAEL S S; ALMEIDA,MARCELA C; MANOEL,CRISTIANO V; SANTOS-FILHO,SEBASTIÃO D; FONSECA,ADENILSON S; FILHO,MARIO BERNARDO.
Stannous chloride (SnC12) is used in nuclear medicine as a reducing agent to obtain technetium-99m-radiopharmaceuticals. It have been reported that natural products might reduce the genotoxic and cytotoxic effects related to SnC12. This work evaluated the biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli (E. coli) AB1157 (wild type) cultures submitted to the action of SnC12. E. coli AB1157 cultures (exponential growth phase) were collected by centrifugation, washed and resuspended in 0.9%NaCl. Samples were incubated in water bath shaker with: (a) SnC12 (25mg/ml), (b)Salix alba extract(11.6mg/ml) and (c)SnC12(25mg/ml) + Salix alba extract (11.6mg/ml). Incubation with 0.9% NaCl was also carried out (control). At 60...
Tipo: Journal article
Palavras-chave: Escherichia coli; Free radicals; Salix alba; Stannous chloride.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200008

Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli
Provedor de dados: 52
Autores: Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de.
Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020

Evaluation of possible failure of the mononuclear phagocyte system after total splenectomy in rats
Provedor de dados: 52
Autores: Marques,Ruy Garcia; Petroianu,Andy; Oliveira,Márcia Betânia Nunes de; Bernardo-Filho,Mário; Portela,Margareth Crisóstomo.
Young and adult Wistar rats were submitted to total splenectomy and compared to animals not submitted to any surgical manipulation in order to evaluate the phagocytic function of spleen. The animals were infected with Escherichia coli labeled with technetium-99m and killed 20 minutes later. Liver, lung, spleen and a blood clot sample were taken. No significant differences were found in the percentage of bacterial radioactivity uptake in mononuclear phagocyte system (MPS) organs in young and adult splenectomized rats. However, phagocytosis index by macrophages of MPS organs was smaller in splenectomized animals than in control group. Splenectomized rats were associated with a higher blood bacterial radioactivity uptake than animals of the control group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spleen; Splenectomy; Technetium-99m; Escherichia coli; Phagocytosis; Mononuclear phagocyte system.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200006

Escherichia Coli: Characteristics of Carbapenem Resistance and Virulence Factors
Provedor de dados: 52
Autores: Candan,Esra Deniz; Aksöz,Nilüfer.
ABSTRACT In this study, fifty Escherichia coli strains were analyzed by multiplex polymerase chain reaction for the genes expressed carbapenemase and virulence factors in order to determine the presence of carbapenemase and nine virulence factors and investigate the association between these two characteristics. When carbapenemase susceptibility was taken into consideration, OXA-48 type carbapenemase was determined for 22% of the total strains. Also, the frequency of virulence gene regions in E.coli infections and virulence gene profiles of these isolates were examined and the frequency of pap, afa, sfa, fimA, iroN, aer, iutA, hly and cnf-1 genes were 24, 38, 20, 84, 28, 90, 92, 10 and 34% respectively. A significant correlation was found between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbapenemase; Escherichia coli; Multiplex polymerase chain reaction; Virulence factor.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100415

The effect of an extract from Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m and on the survival of Escherichia coli
Provedor de dados: 52
Autores: Agostinho,Raquel Terra; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Missailidis,Sotiris; Bernardo-Filho,Mario.
This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m (99mTc) and on the survival of cultures of Escherichia coli treated with stannous chloride. Blood samples from Wistar rats were treated with reishi extract, radiolabeling procedure was performed, plasma (P), blood cells (BC) and insoluble (IF) and soluble (SF) fractions of P and BC were separated. The radioactivity was counted for the determination of the percentages of radioactivity (%ATI). Cultures of Escherichia coli AB1157 were treated with stannous chloride in the presence and absence of reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that reishi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood constituents; Escherichia coli; Ganoderma lucidum; Stannous chloride; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700025

Escherichia coli in the surface waters and in oysters of two cultivations of Guaratuba Bay - Paraná - Brazil
Provedor de dados: 52
Autores: Forcelini,Helenita Catharina Dalla-Lana; Kolm,Hedda Elisabeth; Absher,Theresinha Monteiro.
The present work aimed to evaluate the contamination of Escherichia coli in the surface waters and oysters from two cultivations of Guaratuba Bay and to analyze the correlation patterns among the concentrations of E. coli in the waters and in the oysters with the local physical-chemical parameters. Samples were collected in the spring of 2007 and summer, autumn and winter of 2008 from two points of the bay (internal point and external point). From each cultivation and sampling period, 18 oysters were collected. The samples of surface water were collected for the measurement of physical-chemical parameters (pH, salinity, temperature, dissolved oxygen, seston, particulate organic matter) and quantification of E. coli. The surface water analyzed in the summer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oysters; Escherichia coli; Guaratuba Bay.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200018

Evaluation of Genetic Analysis of ESCHERICHIA COLI Isolated from Two Different Environmental Sources: Sewage Water Verses Soiled Bedding Materials of Laboratory Rodents
Provedor de dados: 52
Autores: Kumar,Sharad; Kumar,Mahadeo; Raj,Abhay; Prakash,Jyoti.
ABSTRACT The present investigation details an assessment of genetic relationship of E. coli isolates collected from two different environmental sources viz. sewage water and soiled bedding materials of laboratory rodents. Five sewage water samples were collected from the industrial area of Lucknow city and 5 samples of soiled bedding materials of laboratory animals were collected from Animal facility at CSIR-IITR, Lucknow. For this study Random amplified polymorphic DNA markers (RAPD) was chosen as the molecular fingerprinting method. In this study, 10 RAPD primers were used to evaluate the genetic similarity of E. coli. isolates. The RAPD-PCR fingerprints were analyzed and data were scored as 1, 0 matrix. The generated data were fed on Popgene software...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Genetic similarity; RAPD-PCR and Rodents.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100445

Enterococcus spp. Resistant to Multiple Antimicrobial Drugs and Determination of Fecal Contamination Levels in Mangrove Oysters (Crassostrea rhizophorae)
Provedor de dados: 52
Autores: Rubião,Cynthia Annes; Franco,Robson Maia; Mesquita,Eliana de Fátima Marques de; Miguel,Marco Antonio Lemos; Cabral,Claudius Couto; Fonseca,Ana Beatriz Monteiro.
ABSTRACT The aim of this study was to determine and compare the Most Probable Number (MPN) of Total Coliforms (TC), Escherichia coli and Enterococcus spp. and to characterize the antimicrobial resistance profiles of Enterococcus spp. isolated from oysters collected in the Barra de Guaratiba Mangrove, Rio de Janeiro, Brazil. The enumeration of E. coli has been used to indicate fecal contamination and hygienic-sanitary conditions of bivalve molluscs. Enterococci are capable to transfer several antimicrobial resistance genes to pathogenic bacteria, including those from Gram-negative group. The oysters were bought from local fishermen and a total of 123 individuals were analyzed. The TC, E. coli and Enterococcus spp. MPN mean were 26,300/100 g, 3,260/100 g and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water pollution; Escherichia coli; Enterococcus spp.; Antimicrobial resistance; Bivalve molluscs.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100308

In vitro inhibition of adhesion of Escherichia coli strains by Xylitol
Provedor de dados: 52
Autores: Silva,Annelisa Farah da; Suzuki,Érika Yoko; Ferreira,Aline Siqueira; Oliveira,Murilo Gomes; Silva,Sílvio Silvério da; Raposo,Nádia Rezende Barbosa.
The present study aimed to evaluate xylitol's antimicrobial and anti-adherence activities on Escherichia coli (ATCC 8739) and on another clinical strain enteropathogenic E. coli (EPEC). In vitro minimum inhibitory concentration (MIC) test and adhesion assays were performed using 0.5, 2.5 and 5.0% xylitol. It was found that xylitol did not have antimicrobial properties on these strains. The scanning electron microscopy (SEM) demonstrated that the slides treated with xylitol had a significant reduction in the number of bacilli and the inhibition of microbial adhesion was probably the xylitol's mechanism of action. Xylitol could be a possible alternative on the control of E. coli infections.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial adhesion; Escherichia coli; Scanning electron microscopy; Xylitol.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200003

The impact of food manufacturing practices on food borne diseases
Provedor de dados: 52
Autores: Sousa,Cristina Paiva de.
Food-borne illness is a major international problem and an important cause of reduced economic growth. The contamination of the food supply with the pathogens and its persistence, growth, multiplication and/or toxin production has emerged as an important public health concern. Most of these problems could be controlled with the efforts on the part of the food handlers, whether in a processing plant, a restaurant, and others. In contrast with most chemical hazardous compounds, the concentration of food pathogens changes during the processing, storage, and meal preparation, making it difficult to estimate the number of the microorganisms or the concentration of their toxins at the time of ingestion by the consumer. This review shows main microorganisms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food-borne diseases; Staphylococcus spp.; Escherichia coli; Salmonella spp.; Food handlers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400020

Differential expression of Zymomonas mobilis sucrase genes (sacB and sacC) in Escherichia coli and sucrase mutants of Zymomonas mobilis
Provedor de dados: 52
Autores: Gurunathan,Sangiliyandi; Gunasekaran,Paramasamy.
The sacB and sacC genes encoding levansucrase and extracellular sucrase respectively were independently subcloned in pBluescript (high copy number) and in Z. mobilis-E. coli shuttle vector, pZA22 (low copy number). The expression of these genes were compared under identical background of E. coli and Z. mobilis host. The level of sacB gene expression in E. coli was almost ten fold less than the expression of sacC gene, irrespective of the growth medium or the host strain. In Z. mobilis the expression of sacB and sacC genes was shown to be subject to carbon source dependent regulation. The transcript of sacB and sacC was three fold higher in cells grown on sucrose than in cells grown on glucose/fructose. Northern blot analysis revealed that the transcript...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymomonas mobilis; Escherichia coli; Expression; Levansucrase; Extracellular sucrase.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300001

Influence of an extract of Juglans regia on the growth of Escherichia coli, on the electrophoretic profile of plasmid DNA and on the radiolabeling of blood constituents
Provedor de dados: 52
Autores: Santos-Filho,Sebastião David; Diniz,Claudia Leite; Carmo,Fernanda Santos do; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
The aim of this work was to study the influence of a walnut (Juglans regia) extract on the growth of Escherichia coli (E. coli) AB1157, on the plasmid DNA topology and on the labeling of blood constituents with technetium-99m (99mTc). An E. coli AB1157 culture, in stationary phase, was incubated with walnut and the growth of the culture was evaluated by optical density at 600 nm for 7 hours. Plasmid DNA samples were incubated with SnCl2 in presence or absence of walnut for 40 minutes, 0.8% agarose gel electrophoresis was performed, the gel was stained and the plasmid topological forms were visualized. Blood samples from Wistar rats were incubated with walnut extract and an assay of labeling of blood constituents with technetium-99m (99mTc) was performed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; DNA; Electrophoresis; Stannous chloride; Technetium-99m; Juglans regia.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700026

Experimental peritonitis in horses. Hematological and biochemistry aspects
Provedor de dados: 62
Autores: MENDES,Luiz Cláudio Nogueira; MARQUES,Luiz Carlos; SCHOCKEN-ITURRINO,Ruben Pablo; ÁVILA,Fernando Antônio de; MALHEIROS,Euclides Braga.
Sixteen adult horses were randomly divided into 4 equal groups (GI, GII, GIII and GIV) of 4 animals and each group was injected intraperitoneally with one of the following suspension: Group I, 100 x 10(7) colony-forming units (CFU) of E. coli diluted in 500 ml of 0.9% saline; Group II, 100 x 10(7) CFU of Bacteroides fragilis in 500 ml of 0.9% saline; Group III, 100 x 10(7) CFU of E. coli in combination with 100 x 10(7) CFU of B. fragilis in 500 ml of 0.9% saline; Group IV, 500 ml of 0.9% saline. Leukopenia appeared in all animals inoculated with bacteria within the first six hours of the experiment. After this period, leukocytosis was observed in some inoculated horses. Horses inoculated with pure cultures of either E. coli or B. fragilis demonstrated mild...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peritonitis; Horses; Escherichia coli; Bacteroides fragilis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000200011

Quinolone-resistant Escherichia coli
Provedor de dados: 55
Autores: Ito,Carmen Antonia Sanches; Gales,Ana Cristina; Tognim,Maria Cristina B.; Munerato,Patrícia; Dalla Costa,Libera Maria.
Quinolones (nalidixic acid - NAL, norfloxacin - NOR, ciprofloxacin - CIP and gatifloxacin - GAT) were tested against Escherichia coli isolated from urine (385 patient samples) by disk diffusion (DD) and agar dilution (AD) methods. Fifty-three samples (13.8%) were classified as resistant to at least one of the quinolones tested. CIP and NOR susceptibilities were the same (91.4%) and they were similar to GAT (92.7%). Susceptibility to NAL, detected by the disk diffusion method, was used to predict susceptibility to NOR, CIP and GAT by the agar dilution method. The sensitivity and specificity of NAL were 100% and 95%, respectively. Twelve samples were analyzed for mutations in the quinolone resistance-determining region (QRDR) of the gyrA and parC genes....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Urinary infection; Quinolone resistance; Escherichia coli.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100003

Sex, drugs, bugs, and age: rational selection of empirical therapy for outpatient urinary tract infection in an era of extensive antimicrobial resistance
Provedor de dados: 55
Autores: Rocha,Jaime L.; Tuon,Felipe Francisco; Johnson,James R.
BACKGROUND: Optimal empirical therapy of urinary tract infection requires accurate knowledge of local susceptibility patterns, which may vary with organism and patient characteristics. METHODS: Among 9,798 consecutive, non-duplicate, community-source urine isolates from ambulatory patients &gt; 13 years old, from clinical laboratory and an academic medical center in Curitiba, Brazil (May 1st to December 1st, 2009), susceptibility data for ampicillin, nitrofurantoin, trimethoprim-sulfamethoxazole, gentamicin, fluoroquinolones, and ceftriaxone/cefotaxime were compared with organism and patient gender and age. RESULTS: The female-to-male ratio decreased with age, from 28.1 (among 20-29 year-olds) to 3.3 (among &gt; 80 year-olds). Overall, susceptibility...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract infections; Anti-bacterial agents; Drug resistance bacterial; Fluoroquinolones; Escherichia coli.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200002

Evaluation of the Susceptibility profiles, genetic similarity and presence of qnr gene in Escherichia coli resistant to ciprofloxacin isolated in Brazilian hospitals
Provedor de dados: 55
Autores: Pereira,Andrea S.; Andrade,Soraya S.; Monteiro,Jussimara; Sader,Helio S.; Pignatari,Antonio C.C.; Gales,Ana C.
Increasing quinolone resistance has been reported worldwide, mainly among clinical isolates of Escherichia coli. The objectives of this study were to determine the susceptibility profile, the genetic relatedness, and the prevalence of the qnr gene among ciprofloxacin-resistant Escherichia coli isolated from distinct Brazilian hospitals. A total of 144 ciprofloxacin-resistant Escherichia coli were isolated from 17 Brazilian hospitals between January/2002 and June/2003. The antimicrobial susceptibility testing was performed by microdilution according to NCCLS. The presence of the qnr gene was initially screened by colony blotting, and then confirmed by PCR followed by DNA sequencing. Ninety-five urinary ciprofloxacin-resistant Escherichia coli were further...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quinolone resistance; Escherichia coli; PFGE; Qnr.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100011

Multidrug-resistant urinary tract isolates of escherichia coli from Ribeirão Preto, São Paulo, Brazil
Provedor de dados: 55
Autores: Santo,Edilene; Salvador,Miriam Mendonça; Marin,José Moacir.
Multiple resistances to antimicrobial drugs arising in Escherichia coli isolates may complicate therapeutic management of urinary tract infection (UTI) by this organism. In order to assess the multidrug resistance (MDR) among urinary E. coli isolates, we have tested 11 antimicrobial drugs against 67 isolates from outpatients attended in a tertiary-care teaching hospital and of 78 isolates from a municipal health unit, respectively in Ribeirão Preto, State of São Paulo, Brazil. Seventy-six percent and 22% of the isolates from the tertiary-care hospital and the municipal unit, respectively, were resistant to three or more different classes of agents, and were considered to present MDR. Among the isolates from the hospital patients, 73.0%, 65.0%, 58.0%, 58.0%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract infections; Escherichia coli; Antimicrobial susceptibility; Multidrug-resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600010

Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFlP and DNA sequencing methods
Provedor de dados: 55
Autores: Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva.
Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009

Genetic analysis of the first mcr-1 positive Escherichia coli isolate collected from an outpatient in Chile
Provedor de dados: 55
Autores: Gutiérrez,Camila; Zenis,Javier; Legarraga,Paulette; Cabrera-Pardo,Jaime R.; García,Patricia; Bello-Toledo,Helia; Opazo-Capurro,Andrés; González-Rocha,Gerardo.
ABSTRACT Global dissemination of mcr-like genes represents a serious threat to public health since it jeopardizes the effectiveness of colistin, an antibiotic used as a last-resort treatment against highly antibiotic-resistant bacteria. In 2017, a mcr-1-positive isolate of Escherichia coli was found in Chile for the first time. Herein we report the genetic features of this strain (UCO-457) by whole-genome sequencing (WGS) and conjugation experiments. The UCO-457 strain belonged to ST4204 and carried a 285 kb IncI2-type plasmid containing the mcr-1 gene. Moreover, this plasmid was transferred by conjugation to an E. coli J53 strain at high frequency. The isolate harbored the cma, iroN, and iss virulence genes and did carry resistance genes to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Colistin-resistance; Mcr-1; Escherichia coli; Chile.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300203

Extended spectrum β-lactamase producers among nosocomial Enterobacteriaceae in Latin America
Provedor de dados: 55
Autores: Guzmán-Blanco,Manuel; Labarca,Jaime A.; Villegas,Maria Virginia; Gotuzzo,Eduardo.
To review the epidemiology of nosocomial extended spectrum β-lactamase-producing Enterobacteriaceae in Latin America, a systematic search of the biomedical literature (PubMed) was performed for articles published since 2005. Rates of nosocomial infections caused by extended spectrum β-lactamase-producing Enterobacteriaceae in Latin America have increased since 2005. Up to 32% of Escherichia coli and up to 58% of Klebsiella pneumoniae isolates are extended spectrum β-lactamase-positive, rates that are higher than in other world regions. From a region-wide perspective, 11-25% of E. coli isolates and 45-53% of K. pneumoniae isolates were nonsusceptible to third-generation cephalosporins. At the country level, there was a wide range in Enterobacteriaceae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Klebsiella; Latin America; Bacterial resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400421

Is nosocomial Escherichia coli bacteremia a predictive risk factor for mortality?
Provedor de dados: 55
Autores: Tuon,Felipe F.; Rocha,Jaime L.; Guadagnin,Fernanda A.; Alves,Márcia S.
The objective of this study was to determine risk factors associated with mortality in patients with nosocomial Escherichia coli bacteremia from January 2009 to January 2011. In a retrospective study the medical records of 88 patients over 18 years with nosocomial bacteremia caused by E. coli were analyzed. In univariate analysis several risk factors, including chronic renal failure, altered mental status, leukocytosis, and higher Charlson index of comorbidities were associated with mortality. In multivariate analysis only altered mental status remained independently associated with mortality. Mental confusion can be a risk factor for mortality in patients with E. coli bacteremia.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Nosocomial bacteremia; Escherichia coli; Mortality; Antibiotic therapy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100092

Pharmacodynamic profiling of commonly prescribed antimicrobial drugs against Escherichia coli isolates from urinary tract
Provedor de dados: 55
Autores: Cuba,Gabriel Trova; Pignatari,Antonio Carlos Campos; Patekoski,Katya Silva; Luchesi,Lucimila Jorge; Kiffer,Carlos Roberto Veiga.
Since antimicrobial resistance among uropathogens against current first line agents has affected the management of severe urinary tract infection, we determined the likelihood that antibiotic regimens achieve bactericidal pharmacodynamic exposures using Monte Carlo simulation for five antimicrobials (ciprofloxacin, ceftriaxone, piperacillin/tazobactam, ertapenem, and meropenem) commonly prescribed as initial empirical treatment of inpatients with severe community acquired urinary tract infections. Minimum inhibitory concentration determination by Etest was performed for 205 Brazilian community urinary tract infection Escherichia coli strains from 2008 to 2012 and 74 E. coli bloodstream strains recovered from a surveillance study. Pharmacodynamic exposure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pharmacodynamics; Monte Carlo method; Escherichia coli; Urinary tract infection.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500512

Effects of sub-minimum inhibitory concentrations of ciprofloxacin on biofilm formation and virulence factors of Escherichia coli
Provedor de dados: 55
Autores: Dong,Guofeng; Li,Jiahui; Chen,Lijiang; Bi,Wenzi; Zhang,Xiaoxiao; Liu,Haiyang; Zhi,Xiaoyang; Zhou,Tieli; Cao,Jianming.
ABSTRACT  Objective: To evaluate the influence of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on biofilm formation and virulence factors of Escherichia coli clinical isolates.  Methods: Sub-MICs of CIP were determined using growth curve experiments. The biofilm-forming capacity of E. coli clinical isolates and E. coli ATCC 25922 treated or untreated with sub-MICs of CIP was assessed using a crystal violet staining assay. The biofilm structure of E. coli isolate was assessed with scanning electron microscopy (SEM). The expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were measured using quantification RT-PCR (qRT-PCR) in E. coli isolates and E. coli ATCC 25922.  Results:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm formation; Ciprofloxacin; Escherichia coli; Sub-minimum inhibitory concentration; Virulence.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100015

Antibiotic resistance patterns of pediatric community-acquired urinary infections
Provedor de dados: 55
Autores: Guidoni,Eliana Biondi Medeiros; Berezin,Eitan N.; Nigro,Stanley; Santiago,Nataly A; Benini,Vanda; Toporovski,Julio.
Knowledge about antimicrobial resistance patterns of the etiological agents of urinary tract infections (UTIs) is essential for appropriate therapy. Urinary isolates from symptomatic UTI cases attended at Santa Casa University Hospital of São Paulo from August 1986 to December 1989 and August 2004 to December 2005 were identified by conventional methods. Antimicrobial resistance testing was performed by Kirby Bauer's disc diffusion method. Among the 257 children, E. coli was found in 77%. A high prevalence of resistance was observed against ampicillin and TMP/SMX (55% and 51%). The antibiotic resistance rates for E. coli were: nitrofurantoin (6%), nalidixic acid (14%), 1st generation cephalosporin (13%), 3rd generation cephalosporins (5%), aminoglycosides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract infection; Pediatrics urinary tract infection; Bacterial resistance; Escherichia coli.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400013

Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture
Provedor de dados: 56
Autores: Conceição,R.A.; Ludovico,M.S.; Andrade,C.G.T.J.; Yano,T.
The adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%). In addition, bacteria concentrations of approximately 2 x 10(7) CFU/mL were recovered from Vero cells following an invasion assay. Furthermore, PCR analysis of adhesin genes showed that 98.0% of these SEPEC strains tested positive for fimH, 69.4% for flu, 53.1% for csgA, 38.8% for mat, and 32.7% for iha. Analysis of the invasin genes showed that 16.3% of the SEPEC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Invasiveness; Escherichia coli; Sepsis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500007

Prevalence of diarrheogenic Escherichia coli and rotavirus among children from Botucatu, São Paulo State, Brazil
Provedor de dados: 56
Autores: Rodrigues,J.; Acosta,V.C.; Candeias,J.M.G.; Souza,L.O.; Filho,F.J.C.
In a one-year prospective study carried out to define the role of rotavirus and Escherichia coli in local childhood diarrhea, we determined the prevalence of both agents in 54 diarrheic children attending a health center in Botucatu. Diarrheogenic E. coli (DEC) strains were characterized by O:H serotyping, a search for virulence genetic markers, and assays of adherence to HEp-2 cells. Except for enteroaggregative E. coli (EAEC), no other DEC category was detected in the children's stools. Both EAEC and rotavirus were isolated from 22 of the 54 (41.0%) diarrheic children as single agents or in combination with other enteropathogens. However, when considering the presence of a single agent, EAEC was dominant and isolated from 20.4% of the patients, whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Rotavirus; Enteroaggregative E. coli; Escherichia coli; Seasonality.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100008

Random amplification of polymorphic DNA reveals clonal relationships among enteropathogenic Escherichia coli isolated from non-human primates and humans
Provedor de dados: 56
Autores: Carvalho,V.M.; Irino,K.; Onuma,D.; Castro,A.F. Pestana de.
Enteropathogenic Escherichia coli (EPEC) strains are important agents of infantile diarrhea all over the world, gaining even greater importance in developing countries. EPEC have also been isolated from various animal species, but most isolates belong to serotypes that differ from those recovered from humans. However, it has been demonstrated that several isolates from non-human primates belong to the serogroups and/or serotypes related to those implicated in human disease. The objective of this study was to evaluate the genetic differences between thirteen strains isolated from non-human primates and the same number of strains isolated from human infections. Human isolates belonged to the same serogroup/serotype as the monkey strains and the evaluation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Enteropathogenic Escherichia coli; Attaching and effacing Escherichia coli; Attaching and effacing; Monkey; Random amplified polymorphic DNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200010

A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide
Provedor de dados: 56
Autores: Ramos,C.R.R.; Abreu,P.A.E.; Nascimento,A.L.T.O.; Ho,P.L.
We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The resulting pAE plasmid combined the advantages of both vectors: small size (pRSET), expression of a short 6XHis tag at N-terminus (pET3-His) and a high copy number of plasmid (pRSET). The small size of the vector (2.8 kb) and the high copy number/cell (200-250 copies) facilitate the subcloning and sequencing procedures when compared to the pET system (pET3-His, 4.6 kb and 40-50 copies) and also result in high level expression of recombinant proteins (20 mg purified protein/liter of culture). In addition, the vector pAE enables the expression of a fusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Expression vector; Immobilized metal affinity chromatography; Protein purification.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800001

Antagonic effect of the inhibition of inducible nitric oxide on the mortality of mice acutely infected with Escherichia coli and Bacteroides fragilis
Provedor de dados: 56
Autores: Astolfi,R.S.; Khouri,D.G.; Brandizzi,L.I.V.; Ávila-Campos,M.J.; Andrade Jr.,H.F. de.
Sepsis, the leading cause of death in intensive care units, is associated with overproduction of nitric oxide (NO) due to inducible NO synthase (iNOS), responsible for some of the pathologic changes. Aminoguanidine (AG) is a selective iNOS inhibitor with reported inconsistent actions in sepsis. To investigate the influence of iNOS, we studied models of acute bacterial sepsis using acute challenges with aerobic (Escherichia coli) and anaerobic (Bacteroides fragilis) bacteria in the presence of AG. Six-week-old, 23 g, male and female BALB/c and C57Bl/6j mice, in equal proportions, were inoculated (ip) with bacteria in groups of 4 animals for each dose and each experiment in the absence or presence of AG (50 mg/kg, ip, starting 24 h before challenge and daily...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Sepsis; Experimental model; Bacterial infections; Escherichia coli; Bacteroides fragilis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300006

Effect of the Escherichia coli EMO strain on experimental infection by Salmonella enterica serovar Typhimurium in gnotobiotic mice
Provedor de dados: 56
Autores: Lima-Filho,J.V.M.; Vieira,L.Q.; Arantes,R.M.E.; Nicoli,J.R.
An experimental infection with Salmonella enterica subsp. enterica serovar Typhimurium was evaluated in gnotobiotic mice previously exposed to a plasmid-free non-pathogenic Escherichia coli (EMO strain). Mice were exposed to EMO (experimental) or not (control) 10 days before challenge with Salmonella Typhimurium (10² colony forming units (CFU)/mouse). Survival after challenge was higher (P &lt; 0.05) in the experimental group (16%) than in the control animals (0%). Histopathological examination of the colon and ileum mucosa of the experimental group showed less extensive lesions such as edema, cell inflammatory infiltration and hyperemia. The epithelial cells of the mucosal surface and the production of the mucous layer were also better preserved in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotic; Salmonella; Escherichia coli; Gnotobiotic mice.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700009

Serogroups and virulence genotypes of Escherichia coli isolated from patients with sepsis
Provedor de dados: 56
Autores: Ananias,M.; Yano,T.
Sixty strains of Escherichia coli, isolated by hemoculture, from septicemic Brazilian patients were evaluated to determine their serogroup and invasivity to Vero cells. All 60 patients died within 2 days of hospitalization. Furthermore, the molecular study of the following extraintestinal pathogenic E. coli-associated virulence factor (VF) genes was performed by PCR: i) adhesins: type 1 fimbria (fimH), S fimbria (sfaD/E), P fimbria (papC and papG alleles) and afimbrial adhesin (afaB/C); ii) capsule K1/K5 (kpsMTII); iii) siderophores: aerobactin (iucD), yersiniabactin (fyuA) and salmochelin (iroN); iv) toxins hemolysin (hlyA), necrotizing cytotoxic factor type 1 (cnf1) and secreted autotransporter toxin (sat); v) miscellaneous: brain microvascular...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Sepsis; Serogroups; Virulence genotypes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000008

Modeling the pressure inactivation dynamics of Escherichia coli
Provedor de dados: 56
Autores: Yamamoto,K.; Matsubara,M.; Kawasaki,S.; Bari,M.L.; Kawamoto,S.
Escherichia coli, as a model microorganism, was treated in phosphate-buffered saline under high hydrostatic pressure between 100 and 300 MPa, and the inactivation dynamics was investigated from the viewpoint of predictive microbiology. Inactivation data were curve fitted by typical predictive models: logistic, Gompertz and Weibull functions. Weibull function described the inactivation curve the best. Two parameters of Weibull function were calculated for each holding pressure and their dependence on holding pressure was obtained by interpolation. With the interpolated parameters, inactivation curves were simulated and compared with the experimental data sets.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Predictive microbiology; Pressure; Escherichia coli; Inactivation; Model.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800014

Treatment of hemorrhagic shock with hypertonic saline solution modulates the inflammatory response to live bacteria in lungs
Provedor de dados: 56
Autores: Fernandes,C.I.; Llimona,F.; Godoy,L.C.; Negri,E.M.; Pontieri,V.; Moretti,A.I.; Fernandes,T.R.; Soriano,F.G.; Velasco,I.T.; Souza,H.P.
Shock and resuscitation render patients more susceptible to acute lung injury due to an exacerbated immune response to subsequent inflammatory stimuli. To study the role of innate immunity in this situation, we investigated acute lung injury in an experimental model of ischemia-reperfusion (I-R) followed by an early challenge with live bacteria. Conscious rats (N = 8 in each group) were submitted to controlled hemorrhage and resuscitated with isotonic saline (SS, 0.9% NaCl) or hypertonic saline (HS, 7.5% NaCl) solution, followed by intratracheal or intraperitoneal inoculation of Escherichia coli. After infection, toll-like receptor (TLR) 2 and 4 mRNA expression was monitored by RT-PCR in infected tissues. Plasma levels of tumor necrosis factor α and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toll-like receptors; Immune system; Pneumonia; Escherichia coli; Neutrophils.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000004

Short-lasting systemic and regional benefits of early crystalloid infusion after intravenous inoculation of dogs with live Escherichia coli
Provedor de dados: 56
Autores: Garrido,A.G.; Poli de Figueiredo,L.F.; Cruz Jr.,R.J.; Silva,E.; Rocha e Silva,M.
We investigated the systemic and regional hemodynamic effects of early crystalloid infusion in an experimental model of septic shock induced by intravenous inoculation with live Escherichia coli. Anesthetized dogs received an intravenous infusion of 1.2 x 10(10) cfu/kg live E. coli in 30 min. After 30 min of observation, they were randomized to controls (no fluids; N = 7), or fluid resuscitation with lactated Ringer's solution, 16 ml/kg (N = 7) or 32 ml/kg (N = 7) over 30 min and followed for 120 min. Cardiac index, portal blood flow, mean arterial pressure, systemic and regional oxygen-derived variables, blood lactate, and gastric PCO2 were assessed. Rapid and progressive cardiovascular deterioration with reduction in cardiac output, mean arterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac output; Escherichia coli; Gas tonometry; Portal blood flow; Septic shock.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600009

Low-level red laser therapy alters effects of ultraviolet C radiation on Escherichia coli cells
Provedor de dados: 56
Autores: Canuto,K.S.; Sergio,L.P.S.; Guimarães,O.R.; Geller,M.; Paoli,F.; Fonseca,A.S.
Low-level lasers are used at low power densities and doses according to clinical protocols supplied with laser devices or based on professional practice. Although use of these lasers is increasing in many countries, the molecular mechanisms involved in effects of low-level lasers, mainly on DNA, are controversial. In this study, we evaluated the effects of low-level red lasers on survival, filamentation, and morphology of Escherichia colicells that were exposed to ultraviolet C (UVC) radiation. Exponential and stationary wild-type and uvrA-deficient E. coli cells were exposed to a low-level red laser and in sequence to UVC radiation. Bacterial survival was evaluated to determine the laser protection factor (ratio between the number of viable cells after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Escherichia coli; Laser; Ultraviolet radiation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000012

Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers
Provedor de dados: 56
Autores: Fonseca,A.S.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.
Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T4endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Enzyme; Escherichia coli; Filamentation; Laser.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000011

Thiol-independent activity of a cholesterol-binding enterohemolysin produced by enteropathogenic Escherichia coli
Provedor de dados: 56
Autores: Figueirêdo,P.M.S.; Catani,C.F.; Yano,T.
Enterohemolysin produced by Escherichia coli associated with infant diarrhea showed characteristics similar to those of thiol-activated hemolysins produced by Gram-positive bacteria, including inactivation by cholesterol, lytic activity towards eukaryotic cells and thermoinstability. However, enterohemolysin activity was not inactivated by oxidation or by SH group-blocking agents (1 mM HgCl2, 1 mM iodoacetic acid) and the hemolysin (100 µg/ml) was not lethal to mice, in contrast to the lethality of the thiol-activated hemolysin family to animals. Earlier reports showed that intravenous injection of partially purified streptolysin O preparations (0.2 µg) was rapidly lethal to mice. These results suggest that E. coli enterohemolysin is not a thiol-activated...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Enterohemolysin; Thiol-independent activity; Cholesterol binding.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100008

Systemic and regional hemodynamic effects of enalaprilat infusion in experimental normotensive sepsis
Provedor de dados: 56
Autores: Rahal,L.; Garrido,A.G.; Cruz Jr.,R.J.; Rocha e Silva,M.; Poli-de-Figueiredo,L.F.
Angiotensin-converting enzyme inhibitors have been shown to improve splanchnic perfusion in distinct shock states. We hypothesized that enalaprilat potentiates the benefits of early fluid resuscitation in severe experimental sepsis, particularly in the splanchnic region. Anesthetized and mechanically ventilated mongrel dogs received an intravenous infusion of live Escherichia coli over a period of 30 min. Thereafter, two interventions were performed: fluid infusion (normal saline, 32 mL/kg over 30 min) and enalaprilat infusion (0.02 mg kg-1 min-1 for 60 min) in randomized groups. The following groups were studied: controls (fluid infusion, N = 4), E1 (enalaprilat infusion followed by fluid infusion, N = 5) and E2 (fluid infusion followed by enalaprilat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac output; Escherichia coli; Enalaprilat; Portal vein blood flow; Septic shock.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900008

Etiology of diarrheal infections in children of Porto Velho (Rondonia, Western Amazon region, Brazil)
Provedor de dados: 56
Autores: Orlandi,P.P.; Magalhães,G.F.; Matos,N.B.; Silva,T.; Penatti,M.; Nogueira,P.A.; Pereira da Silva,L.H.
In the present study, 470 children less than 72 months of age and presenting acute diarrhea were examined to identify associated enteropathogenic agents. Viruses were the pathogens most frequently found in stools of infants with diarrhea, including 111 cases of rotavirus (23.6% of the total diarrhea cases) and 30 cases of adenovirus (6.3%). The second group was diarrheogenic Escherichia coli (86 cases, 18.2%), followed by Salmonella sp (44 cases, 9.3%) and Shigella sp (24 cases, 5.1%). Using the PCR technique to differentiate the pathogenic categories of E. coli, it was possible to identify 29 cases (6.1%) of enteropathogenic E. coli (EPEC). Of these, 10 (2.1%) were typical EPEC and 19 (4.0%) atypical EPEC. In addition, there were 26 cases (5.5%) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrheal disease; Enteropathogens; Escherichia coli; Epidemiology; Western Amazon.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400011

Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures
Provedor de dados: 56
Autores: Barboza,L.L.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.; Fonseca,A.S.
Semiconductor laser devices are readily available and practical radiation sources providing wavelength tenability and high monochromaticity. Low-intensity red and near-infrared lasers are considered safe for use in clinical applications. However, adverse effects can occur via free radical generation, and the biological effects of these lasers from unusually high fluences or high doses have not yet been evaluated. Here, we evaluated the survival, filamentation induction and morphology of Escherichia coli cells deficient in repair of oxidative DNA lesions when exposed to low-intensity red and infrared lasers at unusually high fluences. Cultures of wild-type (AB1157), endonuclease III-deficient (JW1625-1), and endonuclease IV-deficient (JW2146-1) E. coli, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Escherichia coli; Filamentation; Laser.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000013

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil
Provedor de dados: 58
Autores: Rodolpho,Daniela; Marin,José Moacir.
Shiga toxigenic Escherichia coli (STEC) has been implicated as the cause of several human diseases. Samples (ground beef, grinding-machines and human hands) from 23 butcheries were assayed for E. coli using standard microbiological methods, and 287 isolates were submitted to polymerase chain reaction for the detection of stx 1, stx 2 and eae genes. Four STEC isolates were recovered, two from ground beef and two from grinding-machines; all harbored the stx 2 gene and were negative for the eae gene. All E. coli isolates including the four STEC were screened for antibiotic resistance. High levels of resistance against several antimicrobial agents were detected; those most commonly observed were to tetracycline (76.6%), amoxicillin (64.1%) and cephalothin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; STEC; Stx 2; Antibiotic resistance; Ground beef; Cattle.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400004

Measuring bacterial cells size with AFM
Provedor de dados: 58
Autores: Osiro,Denise; Bernardes Filho,Rubens; Assis,Odilio Benedito Garrido; Jorge,Lúcio André de Castro; Colnago,Luiz Alberto.
Atomic Force Microscopy (AFM) can be used to obtain high-resolution topographical images of bacteria revealing surface details and cell integrity. During scanning however, the interactions between the AFM probe and the membrane results in distortion of the images. Such distortions or artifacts are the result of geometrical effects related to bacterial cell height, specimen curvature and the AFM probe geometry. The most common artifact in imaging is surface broadening, what can lead to errors in bacterial sizing. Several methods of correction have been proposed to compensate for these artifacts and in this study we describe a simple geometric model for the interaction between the tip (a pyramidal shaped AFM probe) and the bacterium (Escherichia coli JM-109...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atomic force microscopy (AFM); Escherichia coli; Cell dimensions; Bacteria visualization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100040

Antimicrobial activity of some of the south-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila
Provedor de dados: 58
Autores: Indu,M.N.; Hatha,A.A.M.; Abirosh,C.; Harsha,U.; Vivekanandan,G.
Antibacterial activity of extracts of Allium sativum (garlic), Myristica fragrans (nutmeg), Zingiber officinale (ginger), Allium cepa (onion) and Piper nigrum (pepper) has been evaluated against 20 different serogroups of Escherichia coli, 8 serotypes of Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Garlic extract showed excellent antibacterial activity against all the test organisms, except L. monocytogenes. Nutmeg showed good anti-listerial activity, although activity against E. coli and Salmonella were serotype dependent. Both garlic and nutmeg extracts were effective against A. hydrophila. Extracts of ginger showed inhibitory activity against two serogroups of E. coli: as O8 (enterotoxigenic E. coli) and O88 only. Extracts of onion and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spices extract; Antibacterial activity; Escherichia coli; Salmonella; Listeria monocytogenes; Aeromonas hydrophila.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200011

Expression of aggregative adherence to hela cells by Escherichia coli strains isolated from sick horses
Provedor de dados: 58
Autores: Liberatore,Ana Maria Alvim; Tomita,Sandra Kimie; Vieira,Mônica Aparecida Midolli; Toti Jr.,Cyro; Heckmaier,João; Gomes,Tânia Aparecida Tardelli.
The virulence attributes of 56 Escherichia coli strains isolated from sick horses (secretions of uterine cervices; gastrointestinal and lung fragments of necropsy; diarrheic feces, and tracheal washings) was examined by determining their adherence pattern to HeLa cells and searching for the presence of virulence genes of the various E. coli pathotypes. Two non-adherent strains presented astA, which encodes the enteroaggregative E. coli heat-stable toxin. Twenty-seven strains (48.2%) adhered to HeLa cells, 21 (77.8%) of which presented the aggregative adherence pattern (AA) that characterize the Enteroaggregative E. coli pathotype (EAEC). Nine of the strains presenting AA were isolated from secretions of uterine cervix, including one carrying virulence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Escherichia coli; Aggregative adherence; Virulence.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100003

Microbiological quality of oysters (Crassostrea gigas) produced and commercialized in the coastal region of Florianópolis - Brazil
Provedor de dados: 58
Autores: Pereira,Murilo Anderson; Nunes,Márcia Menezes; Nuernberg,Leonardo; Schulz,Denys; Batista,Cleide Rosana Vieira.
Oysters are filter feeders able to ingest particles in suspension that may carry pathogenic microorganisms. In this respect, the consumption of raw oysters can cause foodborne diseases in humans. The aim of the present study was to evaluate the microbiological quality of Crassostrea gigas oysters cultivated and commercialized in the coastal region of Florianópolis, SC, Brazil. The study comprised counts of coliforms at 35ºC and at 45ºC, Escherichia coli and coagulase-positive staphylococci, and detection of Salmonella sp, Vibrio cholerae and Vibrio parahaemolyticus. Ninety samples were analyzed, 45 of them collected at seafood commercial establishments and the other 45 were collected in the cultivation area. All analyses were performed according to methods...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coliforms; Escherichia coli; Food; Microbiological quality; Oysters.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200012

Presence of extraintestinal pathogenic Escherichia coli in butcheries in Taquaritinga, SP, Brazil
Provedor de dados: 58
Autores: Santo,Edilene; Rodolpho,Daniela; Marin,José Moacir.
The study was conducted in twenty-three butcheries in the city of Taquaritinga, State of São Paulo, Brazil, surveyed during a 10 months period. Among two hundred and eighty-seven Escherichia coli strains isolated from samples of ground beef, meat-grinding-machines and the hands of manipulators, five were recognized as extraintestinal pathogenic E. coli (ExPEC), showing virulence factors (P and S fimbriae, hemolysin and aerobactin) and presenting multidrug resistance. Retail-sold food may constitute an important vehicle for the dissemination of ExPEC in communities, giving rise to reasons for concern.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; ExPEC; Ground beef; Butchery.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400002

Microalgae population dynamics in photobioreactors with secondary sewage effluent as culture medium
Provedor de dados: 58
Autores: Marchello,Adriano E.; Lombardi,Ana T.; Dellamano-Oliveira,Maria José; Souza,Clovis W.O. de.
Nitrogen and phosphorus present in sewage can be used for microalgae growth, possibiliting cost reduction in the production of microalgae at the same time that it decreases the eutrophication potential of the effluent. This research aimed at monitoring the native community of microalgae and coliform bacteria in a secondary effluent from anaerobic municipal sewage treatment. Two treatments (aerated and non-aerated) were performed to grow microalgae under semi-controlled conditions in semi-closed photobioreactors in a greenhouse. The results showed no significant pH and coliforms (total and Escherichia coli) variation between treatments. Nutrient concentrations were reduced supporting microalgae growth up to 107 cells.mL−1independent of aeration. Exponential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microalgae; Effluent treatment; Coliforms; Escherichia coli; Chlorella vulgaris.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100075

Depuration Times of Donax trunculus and Tapes decussatus
Provedor de dados: 58
Autores: Çolakolu,Fatma Arik; Çardak,Mine; Çolakoglu,Serhat; Künili,Ibrahim Ender.
The present study was performed to determine the depuration time and ability of Donax trunculus (Wedge Clam) and Tapes decussatus (Carpet Shell) contaminated with Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium and Vibrio parahaemolyticus. Clams were contaminated with each bacterium at the level of 7.0 - 8.0 Log10 cfu/g. After contamination, clams were analyzed every 3 h in the first 24 h time period and every 6 h until the 72nd hour. During the depuration process of both clams, the level of bacteria decreased quickly to 40% of initial load in the first 12 h. The results of this study indicate that the depuration time of carpet shells for all bacteria is 66 h. The depuration process of the wedge clam was different from the carpet...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clam; Purification; Vibrio parahaemolyticus; Escherichia coli; Salmonella typhimurium.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300034

Evaluation of Petrifilm™ EC and HS for total coliforms and Escherichia coli enumeration in water
Provedor de dados: 58
Autores: Beloti,Vanerli; Souza,Juliana Aparecida de; Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de.
Petrifilm™ EC and HS were compared to the MPN method to determine their efficacy to enumerate total coliforms and E. coli in 145 samples of water (76 water in natura, 69 drinking water). For water in natura, Petrifilm™ HS and EC showed good correlation with MPN method. In chlorinated water (&lt; 20 col/100 mL and negatives) the agreement was low. For E.coli enumeration, Petrifilm™ EC showed a good correlation with MPN method. The results indicated that Petrifilm™ EC and HS are accurate to enumerate coliforms and E. coli in water when the expected counts are higher than 20/100 mL.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Water; Coliforms; Escherichia coli; Petrifilm™.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400002

Prevalence of antimicrobial resistance and integrons in Escherichia Coli from Punjab, Pakistan
Provedor de dados: 58
Autores: Muhammad,Idrees; Uzma,Mussarat; Yasmin,Badshah; Mehmood,Qadir; Habib,Bokhari.
Antimicrobial resistance was studied in Escherichia coli strains isolated from urine samples of 457 patients suffering from urinary tract infection. High prevalence of class 1 integrons (43.56%), sulfamethoxazole resistance genes sul1 (45.54%) and sul2 (51.48%) along with occurrence of quinolone resistance genes was detected in multi drug resistance isolates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Integrons; Antimicrobial resistance; Urinary tract infection.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200008

Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile
Provedor de dados: 58
Autores: Paula,Cleber Jacob Silva de; Marin,José Moacir.
From January to December 2006, 92 Escherichia coli isolates from 25 diarrheic dogs were analyzed by screening for the presence of adhesin-encoding genes (pap, sfa, afa), hemolysin and aerobactin genes. Virulence gene frequencies detected in those isolates were: 12% pap, 1% sfa, 10% hemolysin and 6.5% aerobactin. Ten isolates were characterized as extraintestinal pathogenic E. coli (ExPEC) strains; all showed a multidrug resistance phenotype that may represent a reason for concern due the risk of dissemination of antimicrobial resistant genes to the microbiota of human beings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; ExPEC; Dog; Antimicrobial resistance; Multidrug resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300016

Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms
Provedor de dados: 58
Autores: Vicente,Hinig Isa Godoy; Amaral,Luiz Augusto do; Cerqueira,Aloysio de Mello Figueiredo.
The objective of this study was to determine the prevalence of Shigatoxigenic Escherichia coli (STEC) and STEC serogroups O157, O111 and O113 in feces, water and milk sampled in dairy farms in Jaboticabal, SP, Brazil. Feces (n=454), water (n=54) and milk samples (n=30) were collected from 10 herds and assessed for the presence of the virulence genes stx1, stx2 and eae by polymerase chain reaction (PCR). All stx and eae positive samples were submitted to a second PCR reaction targeting the sequences rfb O157, rfb O111 and rfb O113. High prevalence of stx was detected (59.9%) in fecal samples, whereas the prevalence of sequences rfb O157, rfb O111 and rfb O113 was 18.9%, 3.3% and 30.4%, respectively. All sequences were detected more frequently in calves and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shigatoxigenic; Escherichia coli; Milk; Water; Bovine feces.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300003

Antimicrobial resistance and genetic diversity of Escherichia coli isolated from humans and foods
Provedor de dados: 58
Autores: Melo,Daniela Benevides; Menezes,Ana Paula de Oliveira; Reis,Joice Neves; Guimarães,Alaíse Gil.
Antibiotic resistance has increased in recent years, raising the concern of public health authorities. We conducted a study of Escherichia coli isolates obtained from human and food samples to assess the prevalence of antimicrobial resistance and to determine the genotype and clonal relationship of 84 E. coli isolates (48 from humans and 36 from foods). An antimicrobial susceptibility test was performed using the disk diffusion method. Virulence factors were evaluated by multiplex PCR, and the clonal relationship among the resistant isolates was studied by Pulsed Field Gel Electrophoresis (PFGE). All isolates were susceptible to ceftriaxone. Overall, 26%, 20.2%, 15.4% and 6% of the isolates were resistant to tetracycline, ampicillin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Antimicrobial resistance; Food contamination; Genetic diversity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401165

Microbiological quality of organic vegetables produced in soil treated with different types of manure and mineral fertilizer
Provedor de dados: 58
Autores: Machado,Débora Cabral; Maia,Carla Marques; Carvalho,Isabel Dias; Silva,Natan Fontoura da; André,Maria Cláudia Dantas Porfírio Borges; Serafini,Álvaro Bisol.
An attempt was made to evaluate microbiological quality of horticultural crops grown organically. Three species of vegetables were used, lettuce (Lactuva sativa), radish (Raphanus sativus) and spinach (Tetragonia expansa), grown organically, in fertile soil. Six different treatments were applied: mineral fertilizer, chicken, cow, and pig manure, chicken litter and cow manure, in association with a liquid foliar biofertilizer. These crops were handled according to correct agronomic practices for growing crops organically for commercial purposes. Samples were examined for the Most Probable Number (MPN/g/mL) of total and fecal coliforms and to detect the presence of Escherichia coli and Salmonella spp. All analyzed samples were considered acceptable for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic vegetables; Animal manure; Coliforms; Escherichia coli; Salmonella.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400025

pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics
Provedor de dados: 58
Autores: Hernandez-Flores,Jose Luis; Pérez,Juan Caballero; Gutiérrez,Carlos Saldaña; Hernández,Andrés Cruz; Alonso,Gerardo Soto; Hernández,Sergio Pacheco; Gómez,Sergio Romero; Fernández,Francisco; Loske,Achim M.; Guillén,Juan Campos.
Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Plasmid; Antibiotic resistance; BlaCTX-M-14 gene.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300569

Occurrence of adhesin-encoding operons in Escherichia coli isolated from breeders with salpingitis and chicks with omphalitis
Provedor de dados: 58
Autores: Knöbl,Terezinha; Gomes,Tânia A. Tardelli; Vieira,Mônica A. Midolli; Bottino,José A.; Ferreira,Antonio J. Piantino.
The occurrence of fim, pap and sfa operons in Escherichia coli isolated from breeders with salpingitis and chicks with omphalitis was evaluated. Analysis of 100 E. coli isolates, by colony hybridization tests, showed that 78 (78%) were fim+, one (1%) was sfa+, seven (7%) were fim+ associated with pap+, eigth (8%) were fim+ and sfa+, one (1%) was fim+pap+sfa+ and five (5%) isolates did not hybridize with any probe. These results suggest that fim adhesion-encoding operon plays an important role in pathogenesis of E. coli infection in chickens with salpingitis and omphalitis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Adhesion-encoding operon; Salpingitis; Omphalitis; Poultry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200008

Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
Provedor de dados: 58
Autores: Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Nunes,Maurício Pinto; Santana,Elsa Helena Walter de; Nero,Luís Augusto; Souza,Juliana Aparecida de.
Readycult™ - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult™ - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult™ - LMX. The Readycult™ - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Readycult™ - LMX; X-GAL; MUG; Milk; Escherichia coli; Total coliforms.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010

Action of peracetic acid on Escherichia coli and Staphylococcus aureus in suspension or settled on stainless steel surfaces
Provedor de dados: 58
Autores: Kunigk,Leo; Almeida,Maria C.B.
The efficiency of a commercial peracetic acid sanitizer on destruction of Staphylococcus aureus and Escherichia coli was evaluated using two distinct methods. The first method is the AOAC suspension test and the second is a method proposed by one of the authors in which the microbial cells are settled on a stainless steel surface and then treated with the sanitizer. The results showed that when in suspension S. aureus was more resistant to the sanitizer than E. coli. When S. aureus was settled on the stainless steel surface, the contact time between the sanitizer and the microorganisms to attain a 6.5 log reduction in the number of viable cells was three times greater than when the cells were in suspension.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peracetic acid; Staphylococcus aureus; Escherichia coli; Stainless steel surface; Sanitizers.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100009

A DNA pooling based system to detect Escherichia coli virulence factors in fecal and wastewater samples
Provedor de dados: 58
Autores: Luz María Chacón,J; Lizeth Taylor,C; Carmen Valiente,A; Irene Alvarado,P; Ximena Cortés,B.
The availability of a useful tool for simple and timely detection of the most important virulent varieties of Escherichia coli is indispensable. To this end, bacterial DNA pools which had previously been categorized were obtained from isolated colonies as well as selected in terms of utilized phenotype; the pools were assessed by two PCR Multiplex for the detection of virulent E. coli eaeA, bfpA, stx1, stx2, ipaH, ST, LT, and aatA genes, with the 16S gene used as DNA control. The system was validated with 66 fecal samples and 44 wastewater samples. At least one positive isolate was detected by a virulent gene among the 20 that were screened. The analysis of fecal samples from children younger than 6 years of age detected frequencies of 25% LT positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virulence genes; Wastewater; Fecal; Screening system; Escherichia coli.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400012

Extended-spectrum beta-lactamase production among ampicillin-resistant Escherichia coli strains from chicken in Enugu State, Nigeria
Provedor de dados: 58
Autores: Chah,K.F.; Oboegbulem,S.I.
One hundred and seventy-two ampicillin-resistant E. coli strains isolated from commercial chickens in Enugu State, Nigeria, were screened for beta-lactamase production using the broth method with nitrocefin® as the chromogenic cephalosporin to detect enzyme production. Beta-lactamase producing strains were further examined for extended-spectrum beta-lactamase (ESBL) production using the Oxoid combination discs method. One hundred and seventy (98.8%) of the 172 ampicillin-resistant E. coli strains produced beta-lactamase enzyme. Sixteen (9.4%) beta-lactamase producers were phenotypically confirmed to produce ESBLs. Six of the ESBL producing strains were only detected with ceftazidime versus ceftazidime/clavulanate combination while ten of the ESBL producers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ampicillin-resistant; Escherichia coli; Beta-lactamase; Extended-spectrum.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400018

The relation between Escherichia coli surface functional groups' composition and their physicochemical properties
Provedor de dados: 58
Autores: Hamadi,Fatima; Latrache,Hassan; Zahir,Hafida; Elghmari,Abderrahmene; Timinouni,Mohamed; Ellouali,Mostapha.
Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups' composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups' composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell surface by the (O=C) functional groups. The cell surface's hydrophilicity was associated with high levels of (C-(O.N)) and (OH-(C-O-C)) functional groups, whereas the cell surface's hydrophobicity was associated with (C-(C,H)) functional groups.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Surface functional groups; Hydrophobicity; Electrophoretic mobility.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100003

Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli
Provedor de dados: 58
Autores: Vaz,Michelle Rossana Ferreira; França,Ricardo Luiz Soares de; Andrade,Sirtys Santos Lessa de; Sousa Junior,Francisco Canindé de; Santos,Everaldo Silvino dos; Martins,Daniella Regina Arantes; Macedo,Gorete Ribeiro de.
With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cultivation; Recombinant protein; Visceral leishmaniasis; Escherichia coli.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400021

Antibacterial activity of α-terpineol may induce morphostructural alterations in Escherichia coli
Provedor de dados: 58
Autores: Li,Li; Shi,Chaofeng; Yin,Zhongqiong; Jia,Renyong; Peng,Lianci; Kang,Shuai; Li,Zhengwen.
The antibacterial effect of α-terpineol from Cinnamomum longepaniculatum (Gamble) N. Chao leaf essential oils were studied with special reference to the mechanism of inhibiting the standard strain of Escherichia coli (CMCC (B) 44102) growth at ultrastructural level. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) and time-kill curves of α-terpineol were determined; Escherichia coli was treated with α-terpineol and observed under a transmission electron microscope. The MIC and MBC values of α-terpineol were all 0.78 µL/mL, and time-kill curves showed the concentration-dependent. Under the transmission electron microscopy (TEM), Escherichia coli exposed to MIC levels of α-terpineol exhibited decreased cell size and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-terpineol; Escherichia coli; Antibacterial activity; TEM.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400035

CTX-M extended-spectrum β-lactamase-producing Klebsiella spp, Salmonella spp, Shigella spp and Escherichia coli isolates in Iranian hospitals
Provedor de dados: 58
Autores: Bialvaei,Abed Zahedi; Kafil,Hossein Samadi; Asgharzadeh,Mohammad; Aghazadeh,Mohammad; Yousefi,Mehdi.
Abstract This study was conducted in Iran in order to assess the distribution of CTX-M type ESBLs producing Enterobacteriaceae. From January 2012 to December 2013, totally 198 E. coli, 139 Klebsiella spp, 54 Salmonella spp and 52 Shigella spp from seven hospitals of six provinces in Iran were screened for resistance to extended-spectrum cephalosporins. After identification and susceptibility testing, isolates presenting multiple-drug resistance (MDR) were evaluated for ESBL production by the disk combination method and by Etest using (cefotaxime and cefotaxime plus clavulanic acid). All isolates were also screened for bla CTX-M using conventional PCR. A total of 42.92%, 33.81%, 14.81% and 7.69% of the E. coli, Klebsiella spp, Salmonella spp and Shigella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella spp; Salmonella spp; Shigella spp; Escherichia coli; Multi-drug resistant; ESBL; CTX-M.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300706

Fermentation of sweet whey by recombinant Escherichia coli K011
Provedor de dados: 58
Autores: Leite,Amarildo Ricardo; Guimarães,Walter Vieira; Araújo,Elza Fernandes de; Silva,Daison Olzany.
The production of ethanol from sweet whey using the recombinant Escherichia coli KO11, in batch fermentation, was tested. The maximum ethanol yield was reached after 96h, representing only 38% of the theoretical yield. The supplementation of whey with components of LB broth increased the maximum yield to 96% in 72h. The addition of 0.5% yeast extract to whey resulted in maximum yield of 74% at 36h and it increased to over 100% when yeast extract and trace metals solution (Fe++, Mn++ and Zn++) were added.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Sweet whey; Ethanol.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300011

Phylogenetic grouping and pathotypic comparison of urine and fecal Escherichia coli isolates from children with urinary tract infection
Provedor de dados: 58
Autores: Navidinia,Masoumeh; Peerayeh,Shahin Najar; Fallah,Fatemeh; Bakhshi,Bita; Sajadinia,Raheleh Sadat.
The aim of this study was to investigate the phylogenetic background and to assess hlyD (involved in the secretion of haemolysin A) and intll (encoding a class 1 integrase) in Escherichia coli isolates derived from urinary and fecal specimens. A total of 200 E. coli isolates was collected from patients presenting with urinary tract infection (UTI) during September 2009 to September 2010 and screened for hlyD and intll genes by polymerase chain reaction (PCR). Phylogenetic analysis showed that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D) and that uropathogenic E. coli (UPEC) isolates mainly belong to groups B2 (54%) and D (34%) whereas group A (44%) and D (26%) are predominant among commensal E. coli isolates. In this study, hlyD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Urinary tract infection (UTI); Phylogenetic typing groups; HlyD; Intll.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200019

Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
Provedor de dados: 58
Autores: Scaletsky,Isabel Cristina Affonso; Fabbricotti,Sandra Hilde; Carvalho,Rozane de Lima Bigelli; Nunes,Claudia Regina; Maranhão,Helcio de Sousa; Morais,Mauro Batista de; Riley,Lee; Fagundes-Neto,Ulysses.
Childhood diarrheal diseases remain highly endemic in northeastern Brazil. The attributable fraction of all diarrheal diseases among children less than 2 years of age due to Escherichia coli was examined in a 2-year prospective study in two large urban centers of Brazil. Between May 1997 and June 1999, fecal E. coli isolates from 237 children with diarrhea (217 acute and 20 persistent cases) and 231 children without diarrhea (controls) attending two hospitals in Northeast Brazil were tested for their pattern of adherence to HEp-2 cells and for colony hybridization with DNA probes specific for the six pathotypes of diarrheagenic E. coli. Enteroinvasive E. coli, enterotoxigenic E. coli and enterohemorrhagic E. coli were not isolated from any children....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Diffusely adherent E. coli; Enteroaggregative E. coli; Adherence patterns; Childhood diarrhea.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400012

Characterization of the variable region in the class 1 integron of antimicrobial-resistant Escherichia coli isolated from surface water
Provedor de dados: 58
Autores: Canal,Natália; Meneghetti,Karine Lena; Almeida,Clara Ponzi de; Bastos,Marina da Rosa; Otton,Letícia Muner; Corção,Gertrudes.
Abstract Fecal bacteria are considered to be a potential reservoir of antimicrobial resistance genes in the aquatic environment and could horizontally transfer these genes to autochthonous bacteria when carried on transferable and/or mobile genetic elements. Such circulation of resistance genes constitutes a latent public health hazard. The aim of this study was to characterize the variable region of the class 1 integron and relate its genetic content to resistance patterns observed in antimicrobial-resistant Escherichia coli isolated from the surface waters of Patos Lagoon, Southern Brazil. Genetic diversity of the isolates and presence of the qacEΔ1 gene, which confers resistance to quaternary ammonium compounds, were also investigated. A total of 27...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Class 1 integron; Superficial water; Escherichia coli.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200337

Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
Provedor de dados: 58
Autores: Knöbl,Terezinha; Gomes,Tânia Aparecida Tardelli; Vieira,Mônica Aparecida Midolli; Ferreira,Fernando; Bottino,José Américo; Ferreira,Antônio José Piantino.
Three hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbriae occur with similar frequency in APEC isolated from all Brazilians states, while significant differences among pap and sfa genes distributions were observed. The results showed that 0.85% and 0.28% of APEC were positive for genes that encoded enteroaggregative adhesins and EPEC adherence factor, respectively. None of APEC was positive for DA, afa, Bfp and Eae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: APEC; Escherichia coli; Adhesins; Colibacillosis; Poultry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300033

Effect of ultrasound on survival and growth of Escherichia coli in cactus pear juice during storage
Provedor de dados: 58
Autores: Cruz-Cansino,Nelly del Socorro; Reyes-Hernández,Isidro; Delgado-Olivares,Luis; Jaramillo-Bustos,Diana Pamela; Ariza-Ortega,José Alberto; Ramírez-Moreno,Esther.
Abstract The aim of this study was to investigate the effectiveness of ultrasound as a conservation method for the inactivation of Escherichia coli inoculated into cactus pear juices (green and purple). Total soluble solids, pH, titratable acidity, and the kinetics of E. coli in cactus pear juices treated by ultrasound (60%, 70%, 80% and 90% amplitude levels for 1, 3 and 5 min) were evaluated over 5 days. Total inactivation was observed in both fruit juices after 5 min of ultrasound treatment at most amplitude levels (with the exception of 60% and 80%). After one and two days of storage, the recovery of bacteria counts was observed in all cactus pear juices. Ultrasound treatment at 90% amplitude for 5 min resulted in non-detectable levels of E. coli in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrasound; Growth; Escherichia coli; Cactus pear juice; Storage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200431

Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) strains in ground beef in São Paulo, Brazil
Provedor de dados: 58
Autores: Bergamini,Alzira Maria Morato; Simões,Marise; Irino,Kinue; Gomes,Tânia Aparecida Tardelli; Guth,Beatriz Ernestina Cabilio.
This study aims to assess the prevalence of Shiga toxin-producing Escherichia coli (STEC) in ground beef collected in two cities located in the State of São Paulo, Brazil. A total of 250 samples of raw ground beef were collected in local grocery stores during the period of March to December 2002 in the cities of Ribeirão Preto (114 samples) and Campinas (136 samples), São Paulo State, Brazil. The samples were processed according to standard methods. The resulting 591 E.coli colonies were screened for STEC by hybridization assays using the specific DNA probes, stx1,stx2 and eae. Further characterization of STEC isolates included the search for the ehxA sequence, detection of enterohemolysin and expression of Shiga toxin using the Vero cell assay. STEC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Shiga toxin; STEC; Serotypes; Ground beef.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300032

Molecular genetic differentiation of avian Escherichia coli by RAPD-PCR
Provedor de dados: 58
Autores: Salehi,Taghi Zahraei; Madani,Seyed Ahmad; Karimi,Vahid; Khazaeli,Fatemeh Arab.
Escherichia coli is one of the most important bacterial avian pathogens and a common inhabitant of the gastrointestinal tract of animals. Most pathogenic E. coli can not be differentiated biochemically or by classic microbiologic methods. Molecular typing methods, particularly PCR, facilitated epidemiological and ecological studies of bacteria. Here we describe the application of a random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR) for molecular genetic differentiation of E. coli isolates in Iran. In this study 58 E. coli isolates including 4 standard strains, 3 food originated isolates, 33 avian isolates, 8 isolates form diarrheic calves and 10 isolates from unweaned diarrheic lambs were analyzed by RAPD-PCR using primer 1247(5/-AAG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; RAPD-PCR; Molecular differentiation; Iran.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300015

Quantification of biofilm production on polystyrene by Listeria, Escherichia coli and Staphylococcus aureus isolated from a poultry slaughterhouse
Provedor de dados: 58
Autores: Rodrigues,Laura Beatriz; Santos,Luciana Ruschel dos; Tagliari,Vinícius Zancanaro; Rizzo,Natalie Nadin; Trenhago,Graciela; Oliveira,Amauri Picollo de; Goetz,Franciane; Nascimento,Vladimir Pinheiro do.
This study assessed biofilm formation on polystyrene by Staphylococcus aureus, Listeria monocytogenes, L. welshimeri and Escherichia coli, isolated from a slaughtering plant, grown on tryptic soy broth (TSB) using different glucose concentrations. The tested bacteria produced biofilm in at least one of the concentrations used, and some of them were strong biofilm producers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria; Escherichia coli; Staphylococcus aureus; Biofilm; Polystyrene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400029

Improvement of penicillin G acylase expression in Escherichia coli through UV induced mutations
Provedor de dados: 58
Autores: Arshad,Rubina; Farooq,Shafqat; Ali,Syed Shahid.
We used ultraviolet (UV) radiation to induce mutation in three locally isolated strains of Escherichia coli. Different dilutions of bacterial cultures were exposed to UV lamp of 254 nm wavelength for different time intervals at varied distances ranging from 5 to 210 sec and 5 to 100 cm. Viable colonies were screened for mutants with an increased production of penicillin G acylase (PGA) and a reduced production of β-lactamase, which are the desired properties of PGA producing industrial strains. A survival curve was made to get optimum exposure time and distance. The survival percentage for each exposure period was calculated and 1-5% survival was found useful for obtaining mutants with desired change. Screening for PGA and β-lactamase constitutive and/or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Mutation; Penicillin G acylase expression; UV irradiation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400035

Glucuronidase activity of Escherichia coli isolated from chicken carcasses
Provedor de dados: 58
Autores: Perin,Luana Martins; Yamazi,Anderson Keizo; Moraes,Paula Mendonça; Cossi,Marcus Vinícius Coutinho; Pinto,Paulo Sérgio de Arruda; Nero,Luís Augusto.
To identify Escherichia coli through the production of β-D-glucuronidase (GUD), 622 suspect cultures were isolated from chicken carcasses and plated in PetrifilmTM EC. Of these cultures, only 44 (7.1%) failed to produce GUD. This result indicates the usefulness of GUD production for estimating E. coli populations in chicken.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Glucuronidase activity; Chicken.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300036

Resistance patterns, ESBL genes, and genetic relatedness of Escherichia coli from dogs and owners
Provedor de dados: 58
Autores: Carvalho,A.C.; Barbosa,A.V.; Arais,L.R.; Ribeiro,P.F.; Carneiro,V.C.; Cerqueira,A.M.F.
Abstract Antimicrobial resistance in Escherichia coli isolated from pet dogs can be considered a potential threat of infection for the human population. Our objective was to characterize the resistance pattern, extended spectrum beta-lactamase production and genetic relatedness of multiresistant E. coli strains isolated from dogs (n = 134), their owners (n = 134), and humans who claim to have no contact with dogs (n = 44, control), searching for sharing of strains. The strains were assessed for their genetic relatedness by phylogenetic grouping and pulsed-field gel electrophoresis. Multiresistant E. coli strains were isolated from 42 (31.3%) fecal samples from pairs of dogs and owners, totaling 84 isolates, and from 19 (43.1%) control group subjects. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Antimicrobial resistance; Dogs; Genetic relatedness.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100150

Diarrheagenic Escherichia coli
Provedor de dados: 58
Autores: Gomes,Tânia A.T.; Elias,Waldir P.; Scaletsky,Isabel C.A.; Guth,Beatriz E.C.; Rodrigues,Juliana F.; Piazza,Roxane M.F.; Ferreira,Luís C.S.; Martinez,Marina B.
ABSTRACT Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Diarrhea; Pathogenic mechanisms; Virulence factor; Epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500003

Microbiological and histopathological aspects of canine pyometra
Provedor de dados: 58
Autores: Coggan,Jennifer Anne; Melville,Priscilla Anne; Oliveira,Clair Motos de; Faustino,Marcelo; Moreno,Andréa Micke; Benites,Nilson Roberti.
As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bitches; Escherichia coli; Histopathological; Microbiological; Pyometra.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300012

Survey on pathogenic Escherichia coli and Salmonella spp. in captive cockatiels (Nymphicus hollandicus)
Provedor de dados: 58
Autores: Pontes,Patricia Silveira de; Coutinho,Selene Dall’ Acqua; Iovine,Renata de Oliveira; Cunha,Marcos Paulo Vieira; Knöbl,Terezinha; Carvalho,Vania Maria de.
Abstract We surveyed healthy captive cockatiels (Nymphicus hollandicus) for Escherichia coli and Salmonella spp. Cloacal swabs were collected from 94 cockatiels kept in commercial breeders, private residencies and pet shops in the cities of São Paulo/SP and Niterói/RJ (Brazil). Three strains of E. coli from each individual were tested for the presence of ExPEC-, APEC- and DEC-related genes. We evaluated the blaTEM, blaSHV, blaOXA, blaCMY, blaCTX-M, tetA, tetB, aadA, aphA, strAB, sul1, sul2, sul3, qnrA, qnrD, qnrB, qnrS, oqxAB, aac (6)′-Ib-cr, qepA resistance genes and markers for plasmid incompatibility groups. Salmonella spp. was not detected. E. coli was isolated in 10% of the animals (9/94). Four APEC genes (ironN, ompT, iss and hlyF) were detected in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Salmonella sp; Cockatiel; Nymphicus hollandicus; Antimicrobial resistance.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500076

New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
Provedor de dados: 58
Autores: Xavier,Mauro Aparecido Souza; Kipnis,André; Torres,Fernando Araripe Gonçalves; Astofi-Filho,Spartaco.
We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterologous expression; Escherichia coli; Molecular cloning; Induced expression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400007

Curli fimbria: an Escherichia coli adhesin associated with human cystitis
Provedor de dados: 58
Autores: Cordeiro,Melina Aparecida; Werle,Catierine Hirsch; Milanez,Guilherme Paier; Yano,Tomomasa.
Abstract Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curli fimbria; Adhesion; Escherichia coli; UPEC.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200414

Quality of water sources used as drinking water in a Brazilian peri-urban area
Provedor de dados: 58
Autores: Razzolini,Maria Tereza Pepe; Günther,Wanda Maria Risso; Peternella,Francisca Alzira dos Santos; Martone-Rocha,Solange; Bastos,Veridiana Karmann; Santos,Thaís Filomena da Silva; Cardoso,Maria Regina Alves.
The objective of this paper was to assess bacteriological quality of drinking water in a peri-urban area located in the Metropolitan Region of São Paulo, Brazil. A total of 89 water samples were collected from community plastic tanks and 177 water samples from wells were collected bimonthly, from September 2007 to November 2008, for evaluating bacteriological parameters including: Escherichia coli, Enterococcus and heterotrophic plate count (HPC). Clostridium perfringens was investigated in a subsample (40 samples from community plastic tank and 40 from wells). E. coli was present in 5 (5.6%) samples from community plastic tanks (2.0 - 5.1x10(4) MPN/100mL) and in 70 (39.5%) well samples (2.0 - 8.6x10(4) MPN/100mL). Thus, these samples were not in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Escherichia coli; Enterococcus; Peri-urban area; Drinking water.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200021

Detection of integrons among multi-drug resistant (MDR) Escherichia coli strains isolated from clinical specimens in northern west of Iran
Provedor de dados: 58
Autores: Rezaee,Mohammad Ahangarzadeh; Sheikhalizadeh,Vajihe; Hasani,Alka.
Transference of resistance determinants by integrons is one of the important factors that can contribute to the increase in multi-resistant bacteria. We determined the prevalence and class of integrons among multi-drug resistant (MDR) Escherichia coli strains isolated from clinical specimens in Tabriz teaching hospitals. Firstly, susceptibility of 140 isolates to 13 antibiotics was determined using the disc diffusion method. Then, prevalence and class of integrons was detected in MDR strains by PCR-RFLP. One hundred five (75%) of total 140 isolates were uropathogenic Escherichia coli (UPEC). Other pathotypes included were: diarrheagenic Escherichia coli (13; 9.3%), sepsis-associated E. coli (5; 3.6%) and newborn meningitis-associated E. coli (2; 1.4%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Integron; Escherichia coli; Multi-drug resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400010

Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex
Provedor de dados: 58
Autores: Costa,Clarissa Frota Macatrão; Monteiro Neto,Valério; Santos,Bruno Rafael de Carvalho; Costa,Bruno Rafael Rabelo; Azevedo,Alexandre; Serra,Josilene Lima; Mendes,Hermínio Benítez Rabello; Nascimento,Adenilde Ribeiro; Mendes,Mariana Bonfim Pinto; Kuppinger,Oliver.
The Port Complex of Maranhão (PCM) is the second largest port complex in Brazil, receiving ships with large volumes of ballast water. To evaluate the microbiological quality of its waters, physicochemical parameters (pH and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic Escherichia coli strains were analyzed. In order to identify the presence of E. coli virulence genes target regions of the stx, elt, est, aggR, CVD432, ipaH and eae nucleotide sequences were studied. The presence of totals and thermotolerants coliforms were positive. Analyzing the salinity parameter, a significant increase in total coliforms was observed during the rainy season. We identified the species Escherichia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiological analysis; Coliforms; Enterobacterias; Ballast water; Escherichia coli.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300026

Prevalence of the paa gene (porcine attaching and effacing associated) in porcine enteropathogenic Escherichia coli (PEPEC) associated with postweaning diarrhea in south Brazil
Provedor de dados: 58
Autores: Vidotto,Marilda C.; Florian,Elaine C.T.; Ono,Mario A.
Paa (porcine attaching and effacing associated) may be an important virulence factor E. coli of piglets with diarrhea. This study showed for the first time in Brazil the prevalence of the paa gene (22%) in E. coli strains isolated from piglets and these isolates also harboured genes for other adhesins and toxins LT II, STa and STb.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Pig; PEPEC; Paa gene; Postweaning diarrhea.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200030

Recent updated aspects of colicins of Enterobacteriaceae
Provedor de dados: 58
Autores: Cursino,Luciana; Šmarda,Jan; Chartone-Souza,Edmar; Nascimento,Andréa M.A.
The colicins are protein compounds produced by, and active against, Escherichia coli and others members of Enterobacteriaceae family. At least 34 different colicins have been described and found to share an interesting number of features. In the present review we focus on the major characteristics of colicins of gram-negative bacteria and explore their production and practical applications.The colicins are protein compounds produced by, and active against, Escherichia coli and others members of Enterobacteriaceae family. At least 34 different colicins have been described and found to share an interesting number of features. In the present review we focus on the major characteristics of colicins of gram-negative bacteria and explore their production and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Colicins; Escherichia coli; Genetics; Evolution; Ecology.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300001

Escherichia coli verotoxigênica: isolamento e prevalência em 60 propriedades de bovinos de leite da região de Pelotas, RS, Brasil
Provedor de dados: 65
Autores: Sandrini,Cecília Nunes Moreira; Pereira,Murilo Anderson; Brod,Claudiomar Soares; Carvalhal,José Beiro; Aleixo,José Antonio Guimarães.
A produção de verotoxinas foi investigada em 1.127 isolamentos de Escherichia coli feitos a partir de 243 bovinos de leite, de água de consumo humano e animal e de amostras de leite de 60 propriedades da bacia leiteira de Pelotas, no período de dezembro de 1999 a dezembro de 2000, com o objetivo de determinar a prevalência de E. coli verotoxigênicas (VTEC) nas propriedades e no rebanho, de detectar a presença de sorotipos ligados a infecções humanas e de identificar, nas propriedades e na região de Pelotas, potenciais fatores de risco de infecção para os animais. A detecção das toxinas em sobrenadante de culturas de E. coli isoladas foi realizada através do ensaio de citotoxicidade em células Vero. VTEC foi isolada em 95% (57/60) das propriedades...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Verotoxinas; VTEC; Bovinos.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000100028

Atividade antibacteriana de desinfetantes para uso na produção orgânica de aves
Provedor de dados: 65
Autores: Jaenisch,Fátima Regina Ferreira; Kuchiishi,Suzana Satomi; Coldebella,Arlei.
A determinação de produtos eficazes para a desinfecção e que não causem danos ao meio ambiente é um grande desafio para a avicultura orgânica. Neste trabalho foram avaliadas as atividades antibacterianas de quatro desinfetantes: ácido peracético, amônia quaternária, hipoclorito de sódio a 1% e a 0,1% de cloro ativo e do composto de ácidos orgânicos (cítrico, lático e ascórbico), frente às amostras padrão de Escherichia coli, Salmonella enteritidis e Staphylococcus aureus, na presença e ausência de matéria orgânica, sob duas diferentes temperaturas e tempo de contato de 20 minutos. Os ácidos orgânicos mostraram-se menos efetivos na presença de matéria orgânica. No entanto, o ácido peracético, na ausência desta, foi o mais eficaz frente à S. Enteritidis e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradável; Avicultura orgânica; Escherichia coli; Salmonella Enteritidis; Staphylococcus aureus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200020

Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
Provedor de dados: 65
Autores: Okura,Mõnica Hitomi; Marin,José Moacir.
The soft cheese Minas frescal is one of the most popular cheese in Brazil, which is typically manufactured in small dairy farms under unsatisfactory hygiene conditions. To assess the risk involved in consumption of this cheese, virulence markers were investigated in 330 Escherichia coli strains isolated from 30 Minas frescal cheeses inspected by official government agency (SIF - serviço de inspeção federal), from 50 cheeses not inspected by SIF and 31 cheeses not inspected by SIF with spice added, all of them collected in the southwest of Minas Gerais State. The E. coli isolates were screened for the presence of Shiga toxin-encoding (stx 1 and stx 2), intimin (eae) genes and for the presence of (pap, sfa, afa) genes related to adhesion in epithelial cells....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Cheese; STEC; ExPEC; Antimicrobial resistance; Minas frescal.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801506

Prevalence of enteropathogenic and enterotoxigenic Escherichia Coli in foods of animal origin in southern Brazil
Provedor de dados: 65
Autores: Aleixo,José Antonio; Aver,Gladis Paim.
Prevalence of Escherichia coli in foods of animal origin from the city of Pelotas, Brazil, was determined. The ocurrence of enterotoxigenic (ETEC) and classic enteropathogenic (EPEC) serogroups among E. coli isolates was determined. E. coli was isolated from 68% of the food samples surveyed. Of 36 food samples tested, 11(30%) and 24(66%) were positive for EPEC and ETEC strains respectively. However, of 187 E. coli isolates tested, 30(16%) were EPEC compared to 75(40%) which were ETEC. The antibiotic resistance pattern revealed that the isolates were highly sensitive to all antibiotics tested.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; EPEC; ETEC; Foods; Brazil.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000200013

Microbiologia de queijo tipo Minas Frescal produzido artesanalmente
Provedor de dados: 65
Autores: Loguercio,Andrea Pinto; Aleixo,José Antônio Guimarães.
Com o objetivo de avaliar as condições higiênico-sanitárias do queijo Minas Frescal produzido artesanalmente em Cuiabá - MT, foi realizada análise microbiológica de trinta amostras obtidas em dois pontos de comercialização. A análise microbiológica consistiu da contagem em placas de Staphylococcus aureus e da contagem estimativa de coliformes totais, fecais e Escherichia coli pela técnica do NMP-3 tubos. Na determinação de coliformes fecais, 28 amostras (93,33%) apresentaram número mais provável (NMP) &gt; 10² NMP/g e somente duas amostras (6,67%) estavam dentro dos padrões legais exigidos. Na contagem de S. aureus, em 29 amostras (96,67%) obteve-se valores superiores a 10³ufc/g, estando apenas 1 amostra (3,33%) em conformidade com o padrão legal....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Queijo Minas Frescal; Coliformes; Staphylococcus aureus; Escherichia coli.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000600024

Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
Provedor de dados: 65
Autores: Paula,Cleber Jacob Silva de; Marin,José Moacir.
Shiga toxigenic Escherichia coli (STEC) and Attaching and effacing E. coli (AEEC) have been associated with diarrhea illness in dogs. From January to December 2006, 92 E. coli isolates from 25 diarrheic dogs were analyzed, by screening for the presence of Shiga toxin-producing (stx 1 and stx 2) and intimin (eae) genes. Twelve isolates were detected by PCR to harbor the Shiga toxin genes (7 the stx 1 (7.6%); 5 the stx 2 (5.4%); and none both of them). Nine (9.8%) of the E. coli isolates studied were eae positive non Shiga toxin-producing. Thirteen (62.0%) isolates, carrying stx or eae gene, also showed a hemolysin production. The strains with virulence genes were also examined for resistance to 12 antimicrobial agents. Resistances to cephalothin (85.7%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; STEC; Eae gene; Antimicrobial resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000600029

Motilidade espermática e integridade acrossomal em doses de sêmen suíno refrigeradas e inoculadas com Esecherichia coli e Staphylococcus aureus
Provedor de dados: 65
Autores: Bennemann,Paulo Eduardo; Bortolozzo,Fernando Pandolfo; Wentz,Ivo; Cardoso,Marisa Ribeiro de Itapema.
Foram utilizados 12 ejaculados, coletados de maneira asséptica, distribuídos em sete tratamentos, sendo um grupo controle e os demais inoculados com três diferentes concentrações de S. aureus ou E. coli (5 x 10(5), 5 x 10(6) e 5 x 10(7) UFC/ml). Durante 96 horas, foram avaliados a motilidade espermática, o percentual de acrossomas intactos (NAR), o pH e o número de unidades formadoras de colônia (UFC/ml) das bactérias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com exceção do tratamento com a inoculação de 5 x 10(7) UFC de E. coli/ml, não foi observado efeito significativo das bactérias sobre a motilidade espermática (p&gt;0,05). Da mesma forma, não houve efeito significativo (p&gt;0,05) do S. aureus ou...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sêmen; Acrossoma; Escherichia coli; Staphylococcus aureus.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200019

Single- and Multispecies Biofilms by Escherichia coli, Staphylococcus aureus, and Salmonella spp. Isolated from Raw Fish and a Fish Processing Unit
Provedor de dados: 65
Autores: Frozi,Jesieli Braz; Esper,Luciana Maria Ramires; Franco,Robson Maia.
ABSTRACT:  The consumption of fish by the Brazilian population is increasing. However, fish and seafood are highly perishable and can be contaminated with several microorganisms. In addition, the possibility of biofilm formation is a greater cause for concern. In this study, biofilm formation was evaluated in single- and multispecies cultures at 25°C for incubation periods of 0, 4, 8, 24, and 48h in stainless steel coupons (size, 1.0×1.0cm) immersed in tryptic soy broth. The characteristics of the formed biofilms after sanitizing by immersing the coupons in 200ppm sodium hypochlorite solution for 10min were also evaluated under the same experimental conditions but with some modifications. Biofilm structure was evaluated using scanning electron microscopy....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella spp.; Escherichia coli; Staphylococcus aureus; Biofilm; Stainless steel; Fish industry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000451

Ocorrência de Escherichia coli em meias carcaças de bovinos abatidos em estabelecimento habilitado para exportação
Provedor de dados: 65
Autores: Casagrande,Leandro; Detanico,Camila Menegon Teixeira; Franco,Robson Maia.
A análise de Escherichia coli, considerada bactéria indicadora de contaminação fecal, é utilizada na verificação do controle de processos de abate. Porém, poucos trabalhos foram publicados acerca da ocorrência desse micro-organismo nas indústrias de abate brasileiras. Assim, o presente estudo determinou a ocorrência de E. coli genérica em carcaças bovinas em um estabelecimento sob inspeção federal, habilitado à exportação, identificando as possíveis variações no ano de 2010. Foram coletadas 1111 amostras de suabe de superfície de meias carcaças bovinas e analisadas pela metodologia Petrifilm™. A ocorrência encontrada foi de 4,4% (IC95%=3,3%; 5,7%), com uma média de contagem de 4,08UFC cm-2, não sendo significativamente afetada pelas estações de seca e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abate; Análise bacteriológica; Bovino; Contaminação fecal; Escherichia coli.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000600013

Atividade antimicrobiana de óleos essenciais de condimentos frente a amostras de Escherichia coli isoladas de aves e bovinos
Provedor de dados: 65
Autores: Santurio,Deise Flores; Costa,Mateus Matiuzzi da; Maboni,Grazieli; Cavalheiro,Carlos Pasqualin; Sá,Mariangela Facco de; Dal Pozzo,Marcelo; Alves,Sydney Hartz; Fries,Leadir Lucy Martins.
A discutida questão da substituição do uso de antibacterianos em rações (promotores de crescimento) requer urgentes alternativas. Face às necessidades de inibidores microbianos nesses alimentos, os óleos essenciais (OES) se constituem em alternativa, sob avaliação. Neste estudo, avaliou-se a atividade antimicrobiana dos OES de Origanum vulgare (orégano), Thymus vulgaris (tomilho), Cinnamomum zeylanicum (canela), Lippia graveolens (orégano mexicano), Zingiber officinale (gengibre), Salvia officinalis (sálvia), Rosmarinus officinalis (alecrim) e Ocimum basilicum (manjericão) frente a amostras de Escherichia coli isoladas de fezes de aves (n=43) e de bovinos (n=36). A concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM) foram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Óleos essenciais; Atividade antimicrobiana.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000600021

DIARRÉIA NEONATAL DE ORIGEM BACTERIANA EM BOVINOS
Provedor de dados: 65
Autores: Pianta,Celso.
Diarréia neonatal em bovinos é um quadro complexo, normalmente responsável por altas taxas de morbidade e mortalidade. Os prejuízos são observados não somente nos gastos com a medicação, mas também na perda do animal em caso de morte e na redução do desenvolvimento e do desempenho do futuro animal adulto, comprometendo os índices de ganho de peso ou produção leiteira. A etiologia múltipla da enfermidade envolve a interação de diversas variáveis dos agentes, hospedeiro e meio ambiente. Nesta revisão são abordados os aspectos clínicos da colibacilose sanitário, a prevenção e o tratamento são também abordados.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarréia neonatal; Escherichia coli; Bezerros.
Ano: 1993
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000100021

Antimicrobial susceptibility and pathogenicity of Escherichia coli strains of environmental origin
Provedor de dados: 65
Autores: Carvalho,Daiane; Finkler,Fabrine; Grassotti,Tiela Trapp; Kunert Filho,Hiran Castagnino; Lima,Francisco Esmaile de Sales; Soares,Beatriz Dugaich; Rossato,Juliana Marzari; Cunha,Augusto César da; Brito,Kelly Cristina Tagliari de; Brito,Benito Guimarães de.
The study aimed to evaluate the antimicrobial susceptibility of 109 samples of Escherichia coli (E. coli) of environmental origin and to characterize these isolates according to the degree of pathogenicity in vivo, verifying a possible relationship between this variable and susceptibility to the active principles tested. The isolates were subjected to disc diffusion test to 14 antibiotics. From 16.5% to 90% of the samples were sensitive; 1 - 28.5% showed intermediate degree of susceptibility and between 9 to 78% of E. coli analyzed were resistant. The highest resistance percentages were seen in the class of quinolones and tetracyclines (&amp;gt;75%), and for sensitivity in the class of amphenicols (68.8%). By inoculating 1- day - old chicks, the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Resistance; Antimicrobials; Pathogenicity; Poultry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701249

Feeding different levels of vitamin E and selenium has no effect on serum immunoglobulin Y (IgY) production by layers vaccinated against Escherichia coli and avian encephalomyelitis virus
Provedor de dados: 65
Autores: Kindlein,Giselle; Ribeiro,Andréa Machado Leal; Canal,Cláudio Wageck; Vieira,Maitê de Moraes.
The effects of vitamin E and selenium (Se) supplementation on the immunity of hens vaccinated against a mixture of six swine-pathogenic Escherichia coli (EC) and avian encephalomyelitis virus (AEV) were studied. Antibody production (AbP) was evaluated in ninety 49 to 57-week-old H&amp;N Nick Chick hens fed diets containing 14IU Vitamin E kg-1 (basal diet), 27, 59, 111, or 111IU vitamin E kg-1 + 0.56ppm Se supplementation. At 51 wks of age, half of the hens were vaccinated against EC, and all birds were vaccinated against AEV. At 53-weeks of age, the birds received a second dose of EC vaccine. Blood samples were collected weekly and serum was analyzed by ELISA for anti-EC IgY and was expressed as optical density (OD). Vaccinated hens had higher serum OD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian encephalomyelitis; Escherichia coli; Immunoglobulin; Selenium; Vitamin E.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000500024

Virulence factors and phylotyping of Escherichia coli isolated from non-diarrheic and diarrheic water buffalo calves
Provedor de dados: 65
Autores: Coura,Fernanda Morcatti; Diniz,Soraia de Araújo; Silva,Marcos Xavier; Oliveira,Cairo Henrique Sousa de; Mussi,Jamili Maria Suhet; Oliveira,Camila Stefanie Fonseca de; Lage,Andrey Pereira; Heinemann,Marcos Bryan.
ABSTRACT:  This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One hundred and thirty-four isolates were positive for one or more virulence factors: eighty-four from diarrheic animals, and fifty from non-diarrheic calves. The pathovars of E. coli identified in diarrheic feces were ETEC (F5+) (2/84), NTEC (16/84), STEC (20/84), EPEC (3/84), EHEC (3/84), and EAEC (EAST-1+) (33/84). Pathovars identified in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo calves; Diarrhea; Escherichia coli; Pathovars; Phylogenetic group.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500451

Increase in biofilm formation by Escherichia coli under conditions that mimic the mastitic mammary gland
Provedor de dados: 65
Autores: Costa,João Carlos Miguel; Espeschit,Isis de Freitas; Pieri,Fábio Alessandro; Benjamin,Laércio Anjos; Moreira,Maria Aparecida Scatamburlo.
Bacterial biofilms are involved in the aggravation and recurrence of clinical mastitis in dairy herds. Several factors such as pH, temperature, concentration of O2 and glucose can affect their induction and growth rates. In this study, biofilm production was demonstrated by 27 Escherichia coli strains isolated from bovine mastitis at different pH values depending on the availability of glucose, mimicking conditions found in mammary glands affected by the disease. Biofilm formation was analyzed by spectrophotometric analysis in microtiter plate with 16 different culture media and by scanning electron microscopy. Biofilm formation was greater in isolates cultured under conditions associated with low glucose availability (0.5% or 1.5%) and with either an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mammary gland; Escherichia coli; Milk; Acidity; Sugar.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400015

Clinical aspects of experimental peritonitis in horses
Provedor de dados: 65
Autores: Mendes,Luiz Cláudio Nogueira; Marques,Luiz Carlos; Iturrino,Ruben Pablo Schocken; Ávila,Fernando Antônio de.
Sixteen adult horses were randomly divided into 4 equal groups (GI, GII, GIII and GIV) of 4 animals and each group was injected intraperitoneally with one of the following suspension: GI (100 x 10(7) colony-forming units (CFU) of Escherichia coli diluted in 500ml of 0.9% saline); GII (100 x 10(7) CFU of Bacteroides fragilis in 500ml of 0.9% saline); GIII (100 x 10(7) CFU of E. coli in combination with 100 x 10(7) CFU of B. fragilis in 500ml of 0.9% saline); GIV (500ml of 0.9% saline). Abdominal wall sensitivity to external pressure and tension, diarrhea, decreased intestinal sounds and increased of heart rate were the clinical signs more frequently observed in inoculated horses. Horses inoculated with pure cultures of either E. coli or B. fragilis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peritonitis; Horses; Escherichia coli; Bacteroides fragilis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000300019

Colibacillosis in lambs is associated to type I heat-stable enterotoxin in a farm in São Paulo State, Brazil
Provedor de dados: 65
Autores: Aragão,Annelize Zambon Barbosa; Teocchi,Marcelo Ananias; Fregolente,Maria Clara Duarte; Gatti,Maria Silvia Viccari; Pires,Alexandre Vaz; Yano,Tomomasa.
Twenty seven (48.2%) culture supernatants of 56 Escherichia coli isolated from diarrheic lamb feces (7 to 10 days old) in São Paulo State, Brazil, presented positive results to suckling mice assay (fluid accumulation) but none caused cytopathic effects on Vero and CHO cells, indicating that these strains did not produced LT or VT toxins. PCR assays showed that these 27 E. coli strains harbored estA, that codifies for STa, but not for stx1, stx2 or cnf genes. The positive STa strains were checked for genes that codify for F41, F17 and K99 fimbriae, wich are considered colonization factors in ETEC. Only F17 was detect in two samples (7.4%). Twelve of 27 STa positive carried hlyA gene and presented hemolytic activity in blood Agar. Presence of rotavirus was...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Lambs; STa; Colibacillosis; Escherichia coli.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000500015

Hygienic sanitary conditions of vacuum packed beef produced by slaughterhouses qualified for export in the Mato Grosso state, Brazil
Provedor de dados: 65
Autores: Machado,Maxsueli Aparecida Moura; Müller,Barbara; Carvalho,Ricardo César Tavares; Figueiredo,Eduardo Eustáquio de Souza.
ABSTRACT: Brazil is the largest exporter of beef of the world and Mato Grosso State is the highest beef producer in this country. To maintain product competitiveness and market expansion, sanitary hygienic control of the entire process is indispensable to ensure the attainment of harmless beef and quality. The objective of this study was to evaluate the hygienic sanitary conditions of vacuum-packed beef produced by establishments qualified for export in the state of Mato Grosso, Brazil. A total of 60 samples were submitted to coliforms counts at 35°C and at 45°C and E. coli. The mean contamination by at 35°C and coliforms at 45°C were 3,1 x 102MPN/g and 7.7MPN/g respectively. The presence of E. coli was verified in five samples, representing an occurrence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coliforms; Escherichia coli; Salmonella spp.; Meat.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400451

Inspected and non-inspected chilled chicken carcasses commercialized in Viçosa, MG, Brazil: microbiological parameters and Salmonella spp. occurrence
Provedor de dados: 65
Autores: Cossi,Marcus Vinícius Coutinho; Almeida,Michelle Vieira de; Dias,Mariane Rezende; Pinto,Paulo Sérgio de Arruda; Nero,Luís Augusto.
Sixty samples of chilled chicken carcasses submitted (30) and not submitted (30) to Brazilian inspection services were analyzed to investigate if inspected and non-inspected chilled carcasses represented different food safety risks in the region of Viçosa, MG, Brazil. The mean counts of indicator microorganisms (mesophilic aerobes, Enterobacteriaceae, total coliforms and Escherichia coli) of samples belonging to the inspected and non-inspected lots did not present significant differences (P&amp;gt;0.05). Also, no significant differences (P&amp;gt;0.05) were observed for the numbers of Salmonella spp. and E. coli (higher than 2log cfu g-1) between samples submitted or not to inspection. Statistical differences were observed between the two sample classes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inspection; Salmonella; Escherichia coli; Enterobacteriaceae; Hygiene indicators.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900025

Qualidade higiênica de leite caprino por contagem de coliformes e estafilococos
Provedor de dados: 65
Autores: Gottardi,Carina Philomena Tebisch; Muricy,Renata Fernandes; Cardoso,Marisa; Schmidt,Verônica.
O objetivo deste estudo foi investigar os padrões higiênicos do leite de mistura caprino produzido por oito propriedades associadas a uma cooperativa na região do Vale do Taquari - RS. Foram realizadas duas visitas às propriedades durante o período de maior produção leiteira. As propriedades foram avaliadas quanto às instalações, ao manejo de ordenha e ao armazenamento do leite.Foram coletadas amostras de leite e água em cada visita. As amostras de água apresentaram contagem de coliformes dentro dos limites propostos pela legislação vigente, já as de leite apresentaram contagens de coliformes totais que variaram de zero a 1,4 x 10(6)UFC mL-1. Em apenas duas propriedades foram encontrados coliformes fecais. Não foram identificados estafilococos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leite de rebanho; Leite de cabra; Escherichia coli; Estafilococos coagulase-negativa.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300023

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil
Provedor de dados: 65
Autores: Coura,Fernanda Morcatti; Diniz,Amanda Nadia; Oliveira Junior,Carlos Augusto; Lage,Andrey Pereira; Lobato,Francisco Carlos Faria; Heinemann,Marcos Bryan; Silva,Rodrigo Otávio Silveira.
ABSTRACT: This study identified the virulence genes, pathovars, and phylogenetic groups of Escherichia coli strains obtained from the feces of dogs with and without diarrhea. Virulence genes and phylogenetic group identification were studied using polymerase chain reaction. Thirty-seven E. coli isolates were positive for at least one virulence factor gene. Twenty-one (57.8%) of the positive isolates were isolated from diarrheal feces and sixteen (43.2%) were from the feces of non-diarrheic dogs. Enteropathogenic E. coli (EPEC) were the most frequently (62.2%) detected pathovar in dog feces and were mainly from phylogroup B1 and E. Necrotoxigenic E. coli were detected in 16.2% of the virulence-positive isolates and these contained the cytotoxic necrotizing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Escherichia coli; Pathovars; Phylogroups.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200452

In  vitro efficacy of several disinfectants against  Salmonella enterica serovar Enteritidis and  Escherichia coli strains from poultry
Provedor de dados: 65
Autores: Martínez-Martínez,Sonia; Yubero-Delgado,Sheila; Rodríguez-Ferri,Elías-Fernando; Frandoloso,Rafael; Álvarez-Estrada,Álvaro; Gutiérrez-Martín,César-Bernardo.
ABSTRACT The efficacy of 28 individual or blended disinfectants against avian Salmonella enterica serovar Enteritidis and  Escherichia coli strains was determined. An in vitro test in the presence and absence of serum as source of organic material was conducted. Povidone-iodine (releasing 1% available iodine), 1% potassium permanganate, 70% ethanol, 2% chlorhexidine digluconate and three commercial formulations based on quaternary ammonium compounds + formaldehyde or cresol derivates were the most effective against all strains tested and reduced bacterial counts by more than 106 times (6-log10) regardless of the presence of organic matter. These commercial compounds as well as ethanol and chlorhexidine among the individual substances tested might be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella enterica serovar Enteritidis; Escherichia coli; Disinfection; Suspension test; Poultry industry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801438

Uso do açafrão (Curcuma longa L.) na redução da Escherichia coli (ATCC 25922) e Enterobacter aerogenes (ATCC 13048) em ricota
Provedor de dados: 64
Autores: Maia,Sandra Ribeiro; Ferreira,Ana Cristina; Abreu,Luiz Ronaldo de.
Considerando o envolvimento de queijos como veículo de microrganismos patogênicos, foi avaliada a eficiência do extrato alcoólico de cúrcuma adicionado à ricota, na redução de Escherichia coli e Enterobacter aerogenes. Foram fabricados três lotes de ricota cremosa e inoculados com 104 UFC/mL de Escherichia coli (ATCC 25922) e 105 UFC/mL de Enterobacter aerogenes (ATCC 13048). Às ricotas, foram adicionados 0,4% de NaCl e extrato alcoólico de Curcuma longa L., em concentrações que variaram de 0,0% a 2,0%. As ricotas foram avaliadas físico-química e microbiologicamente em 0, 1, 7, 14 e 21 dias de armazenamento refrigerado. O percentual de umidade das ricotas foi, em média, de 73%. O pH médio observado foi de 5,4 e o percentual de gordura de 3%. Pelos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcuma longa; Escherichia coli; Enterobacter aerogenes; Ricota.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000200016