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Registros recuperados: 251.877
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13CO2 recovery fraction in expired air of septic patients under mechanical ventilation BJMBR
Martins,M.A.; Coletto,F.A.; Martins-Filho,O.A.; Marchini,J.S.; Basile-Filho,A..
The continuous intravenous administration of isotopic bicarbonate (NaH13CO2) has been used for the determination of the retention of the 13CO2 fraction or the 13CO2 recovered in expired air. This determination is important for the calculation of substrate oxidation. The aim of the present study was to evaluate, in critically ill patients with sepsis under mechanical ventilation, the 13CO2 recovery fraction in expired air after continuous intravenous infusion of NaH13CO2 (3.8 µmol/kg diluted in 0.9% saline in ddH2O). A prospective study was conducted on 10 patients with septic shock between the second and fifth day of sepsis evolution (APACHE II, 25.9 ± 7.4). Initially, baseline CO2 was collected and indirect calorimetry was also performed. A primer of 5 mL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Recovery fraction; 13CO2; Stable isotope; Septic shock; Intensive care setting.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700003
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1,3-Diarilpropanos de Iryanthera paraensis e Iryanthera tricornis Acta Amazonica
Silva,José Jerônimo da; Giesbrecht,Astréa M.; Alvarenga,Marden A. de; Gottlieb,Otto R..
Iryanthera paraensis e I. tricornis (Myristicaceae) contêm na madeira de seus troncos 1-(2', 4' - dihidroxifenil) -3- (4", 5"-metilenodoxi-2" -metoxifenil) -propano (1a 1-(2', 4' dihidroxifenil) -3-(3", 4" -metilenodioxifenil)-propano (1b) e 1-(4' -hidroxi -2'-metoxifenil) -3-(4"-hidroxi-3"-metoxifenil) -propano (1c). Apenas da primeira espécie se isolou adicionalmente 1-(2', 4'-dihidroxi-3' -metilfenil)-3-(4", 5"-metilenodioxi-2"- metoxifenil) -propano (1d) e 1-(2'-hidroxi-4'metoxifenil) -3-(3", 4"-metilenodioxifenil) -propano (1e). Dados espectroscópicos para a substância 1b, são descritos pela primeira vez.
Tipo: Info:eu-repo/semantics/article
Ano: 1984 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671984000200455
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1,4- Addition of diazomethane to a heterodiene: a direct preparation of the oxazolic ring Anais da ABC (AABC)
Emery,Flávio da S.; Silva,Raphael S.F.; Moura,Kelly C.G. de; Pinto,Maria C.F.R.; Amorim,Mauro B.; Malta,Valéria R.S.; Santos,Regina H.A.; Honório,Kátia M.; Silva,Albérico B.F. da; Pinto,Antonio V..
The reaction of naphthoquinone-oximes (3) and (4) with diazomethane yields directly, in one step, the oxazoles (5) and (6), respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quinones; Oximes; Diazomethane; Oxazoles.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100004
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14-3-3 proteins in apoptosis BJMBR
Rosenquist,M..
The once obscure members of the 14-3-3 protein family play significant roles in the determination of cell fate. By inhibiting the pro-apoptotic BAD (Bcl-2-antagonist of cell death) and the transcription factor FKHRL-1, 14-3-3 displays important anti-apoptotic characteristics. To date, five points of interaction of 14-3-3 with the apoptotic machinery have been identified. How these interactions are regulated still remains a mystery.
Tipo: Info:eu-repo/semantics/article Palavras-chave: 14-3-3; Apoptosis; Programmed cell death.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400001
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15 anos de CC&T: um breve retrospecto. Repositório Alice
RODRIGUES, C. M..
Este número especial é comemorativo dos 15 anos dos Cadernos de Ciência & Tecnologia, que coincidem também com os 25 anos da Embrapa. O tema escolhido ? propriedade intelectual na agricultura - não poderia expressar melhor a proposta editorial dessa Revista que prima pelo debate e análise crítica de questões contemporâneas inerentes ao desenvolvimento científico e tecnológico. Esse tema é um campo fértil para discussões que polemizam não apenas questões técnicas mas, principalmente, concepções éticas e filosóficas.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ciencia; Science; Tecnologia; Technology.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/83348
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15 de abril dia nacional da conservação do solo: a agricultura desenvolvida no Brasil é conservacionista ou não? Repositório Alice
DENARDIN, J. E.; KOCHHANN, R. A.; FAGANELLO, A..
2011
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Conservação do solo.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/903745
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15N uptake fom manure and fertilizer sources by three consecutive crops under controlled conditions Rev. Bras. Ciênc. Solo
Garza,Héctor Mario Quiroga; Delgado,Jorge A.; Wong,José Antonio Cueto; Lindemann,William C..
There are several regions of the world where soil N analysis and/or N budgets are not used to determine how much N to apply, resulting in higher than needed N inputs, especially when manure is used. One such region is the North Central "La Comarca Lagunera", one of the most important dairy production areas of Mexico. We conducted a unique controlled greenhouse study using 15N fertilizer and 15N isotopic-labeled manure that was labeled under local conditions to monitor N cycling and recovery under higher N inputs. The manure-N treatment was applied only once and was incorporated in the soil before planting the first forage crop at an equivalent rate of 30, 60 and 120 Mg ha-1 dry manure. The 15N treatments were equivalent to 120 and 240 kg ha-1 (NH4)2SO4-N...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Denitrification; Nitrogen use efficiency; Annual ryegrass; Salinity; Sudangrass.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500018
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15N-labed glycine synthesis Anais da ABC (AABC)
Tavares,Claudinéia R.O.; Bendassolli,José A.; Coelho,Fernando; Sant'ana Filho,Carlos R.; Prestes,Clelber V..
This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable 15NH3(aq) volumes in the reaction were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stable isotope; Glycine; Amino acid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300005
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15N-labeled glyphosate synthesis and its practical effectiveness Scientia Agricola
Tavares,Claudinéia Raquel de Oliveira; Bendassolli,José Albertino; Ribeiro,Daniela Neves; Rossete,Alexssandra Luiza Rodrigues Molina.
Glyphosate is the herbicide with the greatest participation in the world market, and it has been used intensively in agriculture for over 30 years, especially because of its relatively low cost and high effectiveness on different species. This study describes a synthesis method of glyphosate in the form of the isopropylamine salt enriched in the stable nitrogen isotope (15N). The 15N-herbicide synthesis was performed using a phosphomethylation reaction with dialkyl phosphite and 15N-glycine. The tests were conducted at a microscale and equimolar quantities. A 20% yield was obtained at the established conditions. To test the effectiveness of the synthesized glyphosate, an experiment was performed in a growth chamber with the weed Lolium multiflorum...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Lolium multiflorum; Isopropylamine salt; Stable isotope.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000100014
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15N-labeled nitrogen from green manure and ammonium sulfate utilization by the sugarcane ratoon Scientia Agricola
Ambrosano,Edmilson José; Trivelin,Paulo Cesar Ocheuze; Cantarella,Heitor; Ambrosano,Gláucia Maria Bovi; Schammass,Eliana Aparecida; Muraoka,Takashi; Rossi,Fabrício.
Legumes as green manure are alternative sources of nitrogen (N) for crops and can supplement or even replace mineral nitrogen fertilization due to their potential for biological nitrogen fixation (BNF). The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulfate (AS) was evaluated using the 15N tracer technique. N was added at the rate of 196 and 70 kg ha-1 as 15Nlabeled sunn hemp green manure (SH) and as ammonium sulfate (AS), respectively. Treatments were: (i) Control; (ii) AS15N; (iii) SH15N + AS; (iv) SH15N; and (v) AS15N + SH. Sugarcane was cultivated for five years and was harvested three times. 15N recovery was evaluated in the two first harvests. In the sum of the three harvests,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum spp.; Brazil; Sunn hemp; Isotope technique.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300014
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16s rDNA-metagenomic sequencing of bone lesions caused by femoral head necrosis in broilers. Repositório Alice
ZANELLA, R.; CANTAO, M. E.; IBELLI, A. M. G.; PEIXOTO, J. de O.; PANDOLFI, J. R. C.; COUTINHO, L. L.; RHOADS, D. D.; AL-RUBAYE, A.; WIDEMAN, R. F.; LEDUR, M. C..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Melhoramento genético animal; Galinha; Animal genetics; Chickens.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1014240
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16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting BJM
Navarro-Noya,Yendi; Hernández-Rodríguez,César; Zenteno,Juan C; Buentello-Volante,Beatriz; Cancino-Díaz,Mario E; Jan-Roblero,Janet; Cancino-Díaz,Juan C.
Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophthalmitis; Staphylococcus epidermidis; PCR; DGGE.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033
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16S rRNA gene-based identification of microbiota associated with the parthenogenetic troglobiont sand fly Deanemyia maruaga (Diptera, Psychodidae) from central Amazon, Brazil BJM
Sousa,Katianne Barbosa Alves de; Silva,Túllio Romão Ribeiro da; Alencar,Ronildo Baiatone; Baton,Luke Anthony; Naveca,Felipe Gomes; Shimabukuro,Paloma Helena Fernandes.
Bacteria associated with the parthenogenetic troglobiont sand fly Deanemyia maruaga were characterized by sequencing cloned 16S rDNA PCR products. Eleven novel partial 16S rDNA sequences, with varying degrees of similarity to Actinobacteria, were identified. None of the sequences identified had homology to those known from parthenogenesis-inducing bacteria.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinobacteria; Bat guano; Parthenogensis; Sand flies; Troglobiont.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100047
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16S rRNA region based PCR protocol for identification and subtyping of Parvimonas micra BJM
Ota-Tsuzuki,C.; Brunheira,A.T.P.; Mayer,M.P.A..
The present study established a PCR protocol in order to identify Parvimonas micra and to evaluate the intra-species diversity by PCR-RFLP of 16S rRNA partial sequence. The data indicated that the protocol was able to identify this species which could be clustered in five genotypes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR-RFLP; 16S rRNA; Anaerobic cocci.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400001
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16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains BJM
Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033
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17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae) Neotropical Ichthyology
Vizziano Cantonnet,Denise; Mateo,Magdalena; Alberro,Andrés; Barrios,Florencia; Fostier,Alexis.
The aim was to investigate the major C21 steroids produced by spermiating white croaker Micropogonias furnieri (Sciaenidae) in order to establish the potential mediator of gamete maturation in males of this species. The testes steroid production at the spawning season was identified incubating the 3H-17-hydroxy-4-pregnene-3,20-dione precursor through thin layer chromatography, high pressure liquid chromatography, enzymatic oxydation, acetylation and immunochemistry analyses. 17,20β-Dihydroxy-4-pregnen-3-one (17,20β-P) and 11β,17,21-Trihydroxy-4-pregnene-3,20-dione (cortisol) were the main metabolites produced. Contrary to what we expected, 17,20β,21-Trihydroxy-4-pregnen-3-one was not detected. Circulating levels of 17,20β-P were undetectable in immature...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Male gonad; Maturing steroids; Reproduction; Steroid synthesis; White croaker.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300613
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18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES BJM
Nascimento,Adriana Mendes do; Martinez-Rossi,Nilce M..
Dermatophytes, capable to use keratin of the host for nutrition, belong to one of the major groups of pathogenic fungi. Since dermatophytes are a closely related group they share various common features, and the morphology of isolates of a given species can be atypical, making species identification and differentiation even more difficult. Many methods have been explored in attempts to distinguish dermatophytes, but the combined use of different approaches for the investigation of the intraspecific and interspecific variability of Trichophyton continues to be scarce. Some studies have shown that amplified fragments of the small ribosomal DNA subunit 18S contains variable regions which can be used to discriminate between medically relevant yeast species,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton; 18S-rDNA sequencing; Extracellular enzymes; Dermatophyte.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300004
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19. Congresso Brasileiro de Economia e Sociologia Rural. Infoteca-e
EMBRAPA. Unidade de Execução de Pesquisa de Âmbito Estadual de Dourados..
1981
Tipo: Outras publicações técnicas (INFOTECA-E) Palavras-chave: Economia rural; Sociologia rural; Congresso; Brasil.
Ano: 1981 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/231085
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19 Congresso Brasileiro de Economia e Sociologia Rural. Infoteca-e
EMBRAPA. Unidade de Execução de Pesquisa de Âmbito Estadual de Dourados..
1981
Tipo: Outras publicações técnicas (INFOTECA-E) Palavras-chave: Economia rural; Sociologia rural; Congresso; Cerrado; Brasil; Agricultural economics; Rural sociology; Congresses; Brazil.
Ano: 1981 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/231143
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19 Congresso Brasileiro de Economia e Sociologia Rural. Infoteca-e
EMBRAPA. Unidade de Execução de Pesquisa de Âmbito Estadual de Dourados..
1981
Tipo: Outras publicações técnicas (INFOTECA-E) Palavras-chave: Economia rural; Sociologia rural; Congresso; Brasil.
Ano: 1981 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/231084
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