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| Tokano,Dorismey Vieira; Kawaichi,Marisa Emiko; Venâncio,Emerson José; Vidotto,Marilda Carlos. |
| The aim of this work was to isolate, clone and characterize the iron uptake gene iutA from avian pathogenic E. coli (APEC). The iutA gene was isolated from the strain APEC 9, serotype O2:H9, which was cloned in the expression vector pET101/D-TOPO. The gene of 2.2 Kb was sequenced (AY602767, which showed high similarity to the iutA gene from three plasmids, two from APEC, pAPEC-02-ColV (AY545598.4) and pTJ100 (AY553855.1), and one from a human invasive E. coli strain, the pColV K30. The recombinant protein IutA was over expressed in E. coli BL21(DE-3) and was solubilized with urea and purified by Ni-NTA column. This method produced a relatively high yield of r-IutA of approximately 74kDa, which was used to produce the antibody anti-IutA. This anti-IutA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian Escherichia coli; Iron uptake; Aerobactin receptor; Virulence factor; IutA gene. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300006 |
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| Tamekuni,Katia; Vidotto,Marilda Carlos; Felix,Samuel Rodrigues; Igarashi,Michelle; Garcia,João Luis; Coelho,Adriana Letícia; Gasparini,Marcela Ribeiro; Marana,Elizabete Regina Marangoni; Silva,Sérgio Silva da; Vidotto,Odilon. |
| This work aims to evaluate the potential of immunization with E. coli BL21 expressing the recombinant rMSP1a and rMSP1b proteins of Anaplasma marginale. E. coli BL21 was transformed with recombinant plasmids pET102/msp1α and pET101/msp1β, and rMSP1a and rMSP1b were expressed after induction by IPTG. BALB/c mice were vaccinated with formolized BL21/rMSP1a and BL21/rMSP1b, and the production in mice sera of whole IgG was determined by ELISA. The mice immunized with BL21/rMSP1a showed a better humoral response for whole IgG when compared to the mice immunized with BL21/rMSP1b; these mice exhibited a small response after the second vaccination. Sera of mice immunized with BL21/rMSP1a reacted via western blot with BL21 and rMSP1a, with molecular masses varying... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anaplasma marginale; MSP1a; MSp1b; Vaccine; Mice; Cattle. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700016 |
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| Alcocer,Iliana; Oliveira,Kelly Mari P. De; Vidotto,Marilda Carlos; Oliveira,Tereza Cristina R.M. de. |
| Salmonelose é a infecção bacteriana de origem alimentar mais freqüente no Paraná, Brasil, e os surtos estão associados, principalmente, ao consumo de ovos, carne de aves e derivados. Os objetivos deste trabalho foram identificar os sorovares de Salmonella isolados de carcaças de frango e caracterizá-los molecularmente por REP e ERIC-PCR, assim como identificar os fagotipos de Salmonella Enteriditis. Dos 25 isolados de Salmonella spp. analisados, 18 foram identificados como Enteriditis, 4 como Braenderup, 2 como Worthington e 1 como infantis. Dos 18 isolados de Enteriditis, 14 foram PT4, 2 PT4a, 1 PT7 e 1 RDNC, por se tratar de colônia rugosa. REP-PCR forneceu padrão eletroforético distinto de 10 a 13 bandas distribuídas entre 120 e 2072 pb para cada... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteriditis; Fagotipos; Sorovar; REP-PCR; ERIC-PCR. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000200026 |
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| Pereira,Daniele Araujo; Vidotto,Marilda Carlos; Nascimento,Karla Alvarenga; Santos,Anne Caroline Ramos dos; Mechler,Marina Lopes; Oliveira,Luís Guilherme de. |
| ABSTRACT: Enterotoxigenic Escherichia coli (ETEC) is the major cause of diarrhea in newborn and weaned pigs. Bacteria adhesion to the host cell is considered a specific phenomenon among fimbrial and non-fimbrial adhesins with their respective receptors on enterocytes. Enteric disorders are related with the fimbriae F4 (K88), F5 (K99), F6 (987P), F41, and F18. In addition to ETEC, another category of E. coli , porcine pathogenic E. coli (PEPEC),can cause diarrhea in pigs; it produces the porcine attaching and effacing-associated (Paa) adhesin in, which is capable to cause a typical lesion known as an attaching and effacing (A/E) lesion. Immunization of sows with adhesin is important to stimulate the production of antibodies and their subsequent transfer... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colibacillosis; Diarrhea; Vaccines; Swine; Colostrum.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801430 |
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