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| Chantret, N.; Salse, J.; Sabot, F.; Rahman, S.; Bellec, A.; Laubin, B.; Dubois, I.; Dossat, C.; Sourdille, P.; Joudrier, P.; Gautier, M.F.; Cattolico, L.; Beckert, M.; Aubourg, S.; Wessenbach, J.; Caboche, M.; Bernard, M.; Leroy, P.; Chalhoub, B.. |
| The Hardness (Ha) locus controls grain hardness in hexaploid wheat (Triticum aestivum) and its relatives (Triticum and Aegilops species) and represents a classical example of a trait whose variation arose from gene loss after polyploidization. In this study, we investigated the molecular basis of the evolutionary events observed at this locus by comparing corresponding sequences of diploid, tertraploid, and hexaploid wheat species (Triticum and Aegilops). Genomic rearrangements, such as transposable element insertions, genomic deletions, duplications, and inversions, were shown to constitute the major differences when the same genomes (i.e., the A, B, or D genomes) were compared between species of different ploidy levels. The comparative analysis allowed... |
| Tipo: Journal Article |
Palavras-chave: GRAIN HARDNESS; GENOME EVOLUTION; DNA RECOMBINATION; WHEAT SPECIES. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20088be18103&uri=/notices/prodinra1/2009/03/ |
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| Alary, R.; Buissonade, C.; Joudrier, P.; Gautier, M.F.. |
| Chestnut and chestnut-derived products are quite expensive and thus a possible target for fraudulent labeling. To obtain a French Label of Origin, chestnut producers need to certify the purity of their products. Chestnut-derived products are consumed by people who may suffer from celiac disease or are allergic to cereals. For these reasons, we developed a qualitative PCR-based method to detect cereal and leguminous species in chestnut flour. The presence of common wheat and barley was determined by amplifying part of the puroindoline-a gene, the presence of rye by amplifying part of the secaloindoline-a gene, the presence of durum wheat, rice, maize and chickpea by amplifying part of lipid transfer protein genes, the presence of oat by amplifying part of a... |
| Tipo: Journal Article |
Palavras-chave: CHESTNUT; CEREALS; PCR; DNA; FOOD LABELING; LEGUMES. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20081cbdceff&uri=/notices/prodinra1/2008/09/ |
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| Evrard, A.; Lagarde, V.; Joudrier, P.; Gautier, M.F.. |
| Puroindolines are two small, basic cysteine-rich proteins isolated from Triticum aestivum seeds and characterized by a tryptophan-rich domain. They form the molecular basis of wheat grain hardness and display antimicrobial activity that may contribute to plant defence. Their antimicrobial activity is presumed to be due to their hydrophobic tryptophan-rich domain. However, little is known about their mode of action and there is no in vivo evidence that the binding of puroindolines to membranes is mediated by their tryptophan-rich domain. In this study, using a yeast complementation assay, we showed that puroindolines interact with the Saccharomyces cerevisiae plasma membrane. By site-directed mutagenesis of their tryptophan-rich domain, we determined that... |
| Tipo: Journal Article |
Palavras-chave: SACCHAROMYCES CEREVISIAE. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20093e8d01c9&uri=/notices/prodinra1/2009/03/ |
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| Chantret, N.; Salse, J.; Sabot, F.; Bellec, A.; Laubin, B.; Dubois, I.; Dossat, C.; Sourdille, P.; Joudrier, P.; Gautier, M.F.; Cattolico, L.; Beckert, M.; Aubourg, S.; Weissenbach, J.; Caboche, M.; Leroy, P.; Bernard, M.; Chalhoub, B.. |
| We study here the evolution of genes located in the same physical locus using the recently sequenced Ha locus in seven wheat genomes in diploid, tetraploid, and hexaploid species and compared them with barley and rice orthologous regions. We investigated both the conservation of microcolinearity and the molecular evolution of genes, including coding and noncoding sequences. Microcolinearity is restricted to two groups of genes (Unknown gene-2, VAMP, BGGP, Gsp-1, and Unknown gene-8 surrounded by several copies of ATPase), almost conserved in rice and barley, but in a different relative position. Highly conserved genes between wheat and rice run along with genes harboring different copy numbers and highly variable sequences between close wheat genomes. The... |
| Tipo: Journal Article |
Palavras-chave: . |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009d515ea76&uri=/notices/prodinra1/2009/03/ |
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| Boutrot, F.; Chantret, N.; Gautier, M.F.. |
| Plant non-specific lipid transfer proteins (nsLTPs) are encoded by multigene families and possess physiological functions that remain unclear. Our objective was to characterize the complete nsLtp gene family in rice and arabidopsis and to perform wheat EST database mining for nsLtp gene discovery.b ResultsIn this study, we carried out a genome-wide analysis of nsLtp gene families in Oryza sativa and Arabidopsis thaliana and identified 52 rice nsLtp genes and 49 arabidopsis nsLtp genes. Here we present a complete overview of the genes and deduced protein features. Tandem duplication repeats, which represent 26 out of the 52 rice nsLtp genes and 18 out of the 49 arabidopsis nsLtp genes identified, support the complexity of the nsLtp gene families in these... |
| Tipo: Journal Article |
Palavras-chave: PROTEINE DE TRANSFERT DES LIPIDES. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200921253611&uri=/notices/prodinra1/2009/03/ |
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