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Registros recuperados: 7
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The application of PCR in the detection of mycotoxigenic fungi in foods BJM
Konietzny,Ursula; Greiner,Ralf.
It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene- as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Fumonisin; Patulin; Polymerase chain reaction; Trichothecene.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400001
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Bacterial phytase: potential application, in vivo function and regulation of its synthesis BJM
Konietzny,Ursula; Greiner,Ralf.
The stepwise release of phosphate from phytate, the major storage form of phosphate in plant seeds and pollen, is initiated by a class of enzymes that have been collectively called phytases. The classification is solely due to the in vitro capability of these enzymes to accept phytate as a substrate. Phytases have been studied intensively in recent years because of the great interest in such enzymes for reducing phytate content in animal feed and food for human consumption. They have a wide distribution in plants, microorganisms, and in some animal tissues. Due to several biological characteristics, such as substrate specificity, resistance to proteolysis and catalytic efficiency, bacterial phytases have considerable potential in commercial applications....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial phytase; Biotechnological application; Dephosphorylation; Occurrence; Phytase formation; Phytate.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100002
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Evaluation of viability of Aspergillus flavus and aflatoxins degradation in irradiated samples of maize BJM
Aquino,Simone; Ferreira,Fabiane; Ribeiro,Deise Helena Baggio; Corrêa,Benedito; Greiner,Ralf; Villavicencio,Anna Lucia Casañas Haasis.
One of the currently most important fungi in stored grains is Aspergillus flavus, which produce aflatoxins. This fungus can grow on diverse substrates and represents a serious public health and animal nutritional problem. Therefore, the study of techniques that can be applied to the control of aflatoxins is of great importance. The objective of the present study was to determine the effects of gamma radiation on the growth of Aspergillus flavus Link and on degradation of aflatoxin B1 and B2 (AFB1 and AFB2) at a relative humidity of 97 99% and a water activity (Aw) of 0.88-0.94. Samples of corn grains were irradiated using a cobalt 60 source emitting gamma rays at doses of 2, 5 and 10 kGy. Irradiation was found to be effective in reducing the number...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma radiation; Aspergillus flavus; Fluorescent viability test; Water activity; Maize.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400009
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Production of D-myo-inositol(1,2,4,5,6)pentakisphosphate using alginate-entrapped recombinant Pantoea agglomerans glucose-1-phosphatase BABT
Greiner,Ralf; Sajidan,.
The glucose-1-phosphatase encoding gene (agp) of Pantoea agglomerans was sequenced and heterologously expressed in Escherichia coli. The enzyme showed very high homology to periplasmatic glucose-1-phosphatases of other members of the Enterobacteriaceae family. It was isolated from transformed Escherichia coli cells in a single step in high yields (32.3 ± 1.2 mg per litre of culture) by Ni-NT agarose affinity chromatography to >95% purity as calculated from specific activity determinations. The purified glucose-1-phosphatase was entrapped in alginate beads with an entrapment efficiency of >80%. Temperature stability was enhanced as a consequence of entrapment, whereas pH dependence of enzyme activity was not affected. Maximum catalytic activity of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entrapment; Glucose-1-phosphatase; Myo-inositol pentakisphosphate Pantoea agglomerans; Phytase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200002
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Purification and characterisation of an extracellular phytase from Aspergillus niger 11T53A9 BJM
Greiner,Ralf; Silva,Lucineia Gomes da; Couri,Sonia.
An extracellular phytase from Aspergillus niger 11T53A9 was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. Purification was achieved by ammonium sulphate precipitation, ion chromataography and gel filtration. The purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kDa and exhibited maximal phytate-degrading activity at pH 5.0. Optimum temperature for the degradation of phytate was 55°C. The kinetic parameters for the hydrolysis of sodium phytate were determined to be K M = 54 µmol l-1 and k cat = 190 sec-1 at pH 5.0 and 37°C. The purified enzyme was rather specific for phytate dephosphorylation. It was shown that the phytase preferably...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Phytate-degrading enzyme; Phytate; Phytase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400010
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Effect of preparation practices and the cowpea cultivar Vigna unguiculata L.Walp on the quality and content of myo-inositol phosphate in akara (fried bean paste) Ciênc. Tecnol. Aliment.
Rogério,Walison Fabio; Greiner,Ralf; Nunes,Itaciara Larroza; Feitosa,Sabrina; Furtunato,Dalva Maria da Nóbrega; Almeida,Deusdélia Teixeira de.
Akara is one of Brazil's national treasures prepared from cowpea (Vigna unguiculata L.Walp), grated onions and salt and deep-fried in crude palm oil. The results of this study on akara preparation methods showed that, in general, cowpeas were soaked for up 3 hours at room temperature, and the seed coats were then removed. The akara makers preferred the olho de pombo cultivar, because of its cream hue, or the macassar cultivar because it produces a crispier paste. The seeds purchased from street markets had lower ranges of InsP6, InsP5, and InsP4 (1.03-7.62 ∝mol.g- 1; 0.14-1.31 ∝mol.g- 1; and 0.0-0.10 ∝mol.g- 1, respectively) than both the paste and akara (6.72-19.24 ∝mol.g- 1; 1.29-4.57 ∝mol.g- 1; 0.0-0.76 ∝mol.g- 1; 3.31-13.71 ∝mol.g- 1; 0.0-4.48 ∝mol.g-...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Akara; Phytate; Frying; Biovailability; Preparation techiniques.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000200004
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Phytase-Producing Bacteria from Extreme Regions in Indonesia BABT
Sajidan,; Wulandari,Rita; Sari,Evy Novita; Ratriyanto,Adi; Weldekiros,Hailu; Greiner,Ralf.
ABSTRACTIn this study, 154 isolates capable of producing extracellular phytate-degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate-degrading activity were selected for strain identification and characterization of the corresponding phytate-degrading enzyme. Blast analysis of 16S rRNA gene sequences revealed high similarities for all the six isolates to reference sequences belonging to the genusBacillus. Isolates MS5, MC6, D10 and D16 showed 99% sequence identity toB. cereus, while isolate MC8 exhibited 99% sequence identity toB. aryabhatti and D6 99% sequence identity toB. psychrotolerans. The crude extracellular phytase preparations from the isolates showed following optimal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rDNA; Bacillus sp. bacterial phytase phytate phytate-degrading enzyme.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500711
Registros recuperados: 7
Primeira ... 1 ... Última
 

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