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The application of PCR in the detection of mycotoxigenic fungi in foods BJM
Konietzny,Ursula; Greiner,Ralf.
It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene- as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Fumonisin; Patulin; Polymerase chain reaction; Trichothecene.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400001
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Bacterial phytase: potential application, in vivo function and regulation of its synthesis BJM
Konietzny,Ursula; Greiner,Ralf.
The stepwise release of phosphate from phytate, the major storage form of phosphate in plant seeds and pollen, is initiated by a class of enzymes that have been collectively called phytases. The classification is solely due to the in vitro capability of these enzymes to accept phytate as a substrate. Phytases have been studied intensively in recent years because of the great interest in such enzymes for reducing phytate content in animal feed and food for human consumption. They have a wide distribution in plants, microorganisms, and in some animal tissues. Due to several biological characteristics, such as substrate specificity, resistance to proteolysis and catalytic efficiency, bacterial phytases have considerable potential in commercial applications....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial phytase; Biotechnological application; Dephosphorylation; Occurrence; Phytase formation; Phytate.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100002
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