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Fungicide application can improve production of tomato coinfected with Begomovirus and Crinivirus PAB
Guimarães,Leysimar Ribeiro Pitzr; Nozaki,Denise Nakada; Moura,Mônika Fecury; Spadotti,David Marques de Almeida; Mituti,Tatiana; Krause-Sakate,Renate; Pavan,Marcelo Agenor.
Abstract: The objective of this work was to evaluate the effect of fungicide application on the concentration of Tomato severe rugose virus (ToSRV, Begomovirus) in the 'Mariana' hybrid tomato coinfected with ToSRV and Tomato chlorosis virus (ToCV, Crinivirus) and the progression of viral concentration by qPCR, as well as to quantify fruit yield and quality. Experiment I consisted in the application of fungicides after sowing (pretreatment): pyraclostrobin+metiram (P+M) (at 3 g L-1) + boscalid (B) (at 0.3 g L-1), followed by biweekly sprayings with P+M (4 g L-1); in experiment II, there was no application at sowing (control treatment), only 4 g L-1 P+M biweekly. ToSRV and ToCV transmissions were performed using sweetpotato whiteflies (Bemisia tabaci,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum lycopersicum; Boscalid; Metiram; Pyraclostrobin; ToCV; ToSRV..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000600435
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Survey of viruses belonging to different genera and species in noble garlic in Brazil Scientia Agricola
Mituti,Tatiana; Moura,Mônika Fecury; Marubayashi,Julio Massaharu; Oliveira,Milena Leite; Imaizumi,Vitor Massami; Sakate,Renate Krause; Pavan,Marcelo Agenor.
Garlic (Allium sativumL.) is a host to several viruses, most commonly those belonging to theAllexivirus,Carlavirus, orPotyvirusgenera. Nine species distributed among these three genera have been reported in Brazil: two species within carlaviruses, two within potyviruses, and five within allexiviruses. To quantify the prevalence of these viruses, young leaves from 520 plants (plants either symptomatic or asymptomatic) were collected from commercial fields grown in four Brazilian states and analyzed using universal and species-specific primers via the reverse transcription polymerase chain reaction (RT-PCR). Potyvirus presence was positive in 306 samples (81 %), 151 of them (38 %) in mixed infections with other viruses. The most frequent potyviruses...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allexivirus; Allium sativum; Carlavirus; Potyvirus; Occurrence.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278
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Reaction of lettuce genotypes to Lettuce mosaic virus-Most (LMV-Most) and characterization of the translation factor eIF4E PAB
Moura,Mônika Fecury; Silva,Norberto da; Hoffmann,Maria Isabel Motta; Pavan,Marcelo Agenor; Krause-Sakate,Renate.
Abstract: The objective of this work was to evaluate lettuce genotypes for their reaction to Lettuce mosaic virus (LMV; Most-type, isolate AF-199) and variations of the eukaryotic translation initiation factor eIF4E. All inoculated genotypes were susceptible to LMV, which was detected by RT-PCR using specific primer pairs. However, the accessions 169501, 169501C, 172918A, and 162499 showed late development of symptoms that appeared only on the inoculated leaves. Sequencing of the coding region of eIF4E showed that these genotypes have an eIF4E0 (mol 0 ) standard typical for their susceptibility to LMV, indicating that the phenotype found is not correlated to nucleotide variations in this translation factor.
Tipo: Info:eu-repo/semantics/article Palavras-chave: EIF4E; Potyvirus; Resistance.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100125
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Only the B biotype of Bemisia tabaci is present on vegetables in São Paulo State, Brazil Scientia Agricola
Rocha,Kelly Cristina Gonçales; Marubayashi,Julio Massaharu; Navas-Castillo,Jesús; Yuki,Valdir Atsushi; Wilcken,Carlos Frederico; Pavan,Marcelo Agenor; Krause-Sakate,Renate.
Bemisia tabaci (Genn.) is one of the most important pests in cultivated areas of vegetables and ornamental crops around the world. Based on the mitochondrial cytochrome oxidize I (mtCOI) sequence, there is evidence that B. tabaci should be considered a cryptic species complex of 11 groups containing 24 species. Two of the groups, Middle East-Asia Minor 1 and Mediterranean include biotypes B and Q, respectively. In this study we evaluated the mtCOI sequence of B. tabaci populations collected in sites of the state of São Paulo, Brazil. Using PCR-RFLP with Taq I, a typical biotype B profile was obtained for all specimens. Based on the comparison with mtCOI reference sequences we found four haplotypes all belonging to the Middle East-Asia Minor 1. They...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RFLP; Cytochrome oxidase I gene; Sequencing; Whiteflies.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000100018
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