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Triacylglycerols composition of walnut (Juglans regia L.) cultivars: characterization by HPLC/ELSD and chemometrics IPB - Escola Superior Agrária
Amaral, J.S.; Cunha, S.C.; Alves, M.R.; Pereira, J.A.; Seabra, R.M.; Oliveira, M.B.P.P..
A total of 26 walnut ( Juglans regia L.) samples from 9 cultivars (Arco, Franquette, Hartley, Lara, Marbot, Mayette, Mellanaise, Parisienne, and Rego) harvested in the 2001, 2002, and 2003 crop years and grown in two geographical origins (Bragancüa and Coimbra, Portugal) were evaluated with regard to their triacylglycerol composition. The methodology employed was reversed-phase highperformance liquid chromatography coupled to an evaporative light-scattering detector (RP-HPLCELSD) after extraction of the lipidic fraction of the nuts. Nine compounds were separated, identified, and quantified. All samples presented an identical qualitative profile composed by LLnLn, LLLn, LLL, OLLn, OLL, PLL, OOL, and PLO (P ) palmitoyl; O ) oleoyl; L ) linoleoyl; Ln )...
Tipo: Article Palavras-chave: Triacylglycerols; Juglans regia L.; Walnut oil; HPLC-ELSD.
Ano: 2004 URL: http://hdl.handle.net/10198/630
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Triphelio – an international research project for sustainable control of lepidopterous pests in olive groves. IPB - Escola Superior Agrária
Agamy, E.; Bento, Albino; Hafez, B.; Hassan, S.A.; Hegazi, E.; Herz, A.; Jardak, T.; Ksantini, M.; Konstantopoulou, M.; Mazomenos, B.; Nasr, F.; Pereira, J.A.; Torres, L.; Youssef, A..
The olive moth (Prays oleae, Lepidoptera: Plutellidae) is known as one of the key pests in olive cultivation. In particular areas, also the jasmine moth (Pal pita zmionalis, Lepidoptera: Pyralidae) can cause serious damage to both leaves and fiuits of the olive tree. Both pests are mainly controlled by insecticide applications. In November 200 I, an international research project was started to promote biological and biotechnical methods for the control of these pests.
Tipo: ConferenceObject
Ano: 2003 URL: http://hdl.handle.net/10198/5341
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Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phenolic compounds and antioxidant potential. IPB - Escola Superior Agrária
Andrade, P.B.; Sousa, C.; Seabra, R.M.; Pereira, J.A.; Ferreres, F..
Tronchuda cabbage (Brassica oleracca L. var. "oslalo DC) seeds were studied for their phenolic composition and antioxidant capacity. Thirteen compounds were characterized and quantified by reversed-phase HPLC-OAO-MSIMSESI and HPLC-OAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucosc isomers, kacmpferol-3- (sinapoyl)sophofOtrioside-7 -gI ucosidc, sinapoyIchol inc; kaempferol-3, 7-diglucoside-4' -(sinapoyl)glucoside, three disinDpoylgentiobiose isomers, 1,2,2' -trisinapoyIgentiobiose and I ,2-disinapoylglucosc. The aqueous extract of tronchuda cabbage seeds WDS investigated for its capacity to act as a scavenger of DPPH radical and reactive oxygen species (superoxide and hydroxyl radicals Dnd hypochlorous acid), exhibiting antioxidant...
Tipo: ConferenceObject
Ano: 2006 URL: http://hdl.handle.net/10198/5373
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Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phytochemical characterization and antioxidant potential. IPB - Escola Superior Agrária
Ferreres, F.; Sousa, C.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Andrade, P.B..
Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS/MS-ESI and HPLC-DAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucose isomers, kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-40-(sinapoyl)glucoside, three disinapoylgentiobiose isomers, 1,2,20-trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified by HPLC-UV. The aqueous extract of tronchuda cabbage seeds was investigated for its capacity to act...
Tipo: Article Palavras-chave: Tronchuda cabbage seeds; Brassica oleracea L. var. costata DC; Phenolic compounds; Organic acids; Antioxidant capacity.
Ano: 2007 URL: http://hdl.handle.net/10198/752
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Tronchuda cabbage flavonoids uptake by Pieris brassicae IPB - Escola Superior Agrária
Ferreres, F.; Sousa, C.; Valentão, P.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B..
The flavonoid pattern of larvae of cabbage white butterfly (Pieris brassicae L.; Lepidoptera: Pieridae) reared on the leaves of tronchuda cabbage was analysed by HPLC-DAD-MS/MS-ESI. Twenty flavonoids were identified or characterised, namely 16 kaempferol and four quercetin derivatives. Kaempferol 3-O-sophoroside, a minor component of tronchuda cabbage, was found to be the main component in P. brassicae (15.8%). Apart from this, only two other flavonoids present in significant amounts in tronchuda cabbage (kaempferol 3-O-sophoroside-7-O-glucoside and kaempferol 3-O-sophoroside-7-O-sophoroside) were found in the larvae. The larvae have high amounts of quercetin derivatives (18.5%), which were present only in trace amounts in tronchuda cabbage extracts,...
Tipo: Article Palavras-chave: Pieris brassicae L. larvae; Tronchuda cabbage; Brassica oleracea L. var. costata DC; Flavonoids.
Ano: 2007 URL: http://hdl.handle.net/10198/745
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Validation of a method to quantify copper and other metals in olive fruits by ETAAS. Application to the residual metal control after olive tree treatments with different copper formulations IPB - Escola Superior Agrária
Soares, Maria Elisa; Pereira, J.A.; Bastos, Maria de Lourdes.
An electrothermal atomization atomic absorption spectrometry method was validated to quantify residues of copper, aluminum, cadmium, chromium, iron, lead, and nickel in olive fruit. The linearity ranges under the optimized conditions were 0.19-20.0, 1.11-50.0, 0.02-2.0, 0.15-20.0, 0.80- 20.0, 0.35-50.0, and 0.60-50.0 íg/L, respectively. The limits of quantification were, expressed in nanograms per gram of dry weight, 12.6, 74.0, 1.34, 10.0, 53.4, 23.4, and 40.0, respectively. For all of the metals the precision of the instrumental method was <6.3% and that of the analytical method was always <10%, except for aluminum, for which the precision was 12%. The accuracy of the method was evaluated according to the standard additions method, the recoveries...
Tipo: Article Palavras-chave: Method validation; Atomic absorption spectrometry; Metal residues; Olive fruit; Fungicide residues.
Ano: 2006 URL: http://hdl.handle.net/10198/748
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Vitamin E characterization in Castanea sativa Mill. cultivars from three consecutive years: a useful authenticity discrimination factor. IPB - Escola Superior Agrária
Barreira, João C.M.; Casal, S.; Ferreira, Isabel C.F.R.; Pereira, J.A.; Oliveira, M.B.P.P..
Vitamin E profiles of selected (2006-2008) Castanea saliva Miller (chestnut) cultivars (Aveleira, Boaventura, Judia and Longal) belonging to the Protected Designation of Origin "Castanha da Terra Fria" were evaluated. Vitamin E is an important lipophilic antioxidant with essential effccts in living systems against aging, strengthening the immune system and reducing cancer risk, reducing viral load in HIV -infected persons or in the treatment of Parkinson-syndrome. The analyses were conducted in NP-HPLC coupled to a multiwavelenght diode array detector connected with a fluorescence detector.
Tipo: ConferenceObject
Ano: 2010 URL: http://hdl.handle.net/10198/3684
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Vitamin E profile as a reliable authenticity discrimination Factor between chestnut (Castanea sativa Mill.) cultivars IPB - Escola Superior Agrária
Barreira, João C.M.; Alves, Rita C.; Casal, S.; Ferreira, Isabel C.F.R.; Oliveira, M.B.P.P.; Pereira, J.A..
In this study, the profile of tocopherols and tocotrienols in chestnut (Castanea sativa Mill.) kernel oil was evaluated. Four Portuguese chestnut varieties were selected: Aveleira, Boaventura, Judia, and Longal. The vitamin E determination had already been applied to similar matrices, but, to the authors’ knowledge, it is the first time that chestnut kernel oil has been evaluated. The prevalent vitamer was γ-tocopherol, often present in trace amounts in other natural products. Due to the high commercial value of chestnut, a statistical analysis of the obtained results was also conducted to define the tocopherol and tocotrienol profile as a reliable indicator of a specific chestnut variety. To achieve this objective, an analysis of variance was performed to...
Tipo: Article Palavras-chave: Chestnut kernel oil; HPLC; Vitamin E; Discriminant analysis.
Ano: 2009 URL: http://hdl.handle.net/10198/2613
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Volatile constituents throughout Brassica oleracea L. var. acephala germination IPB - Escola Superior Agrária
Fernandes, F.; Pinho, P. Guedes de; Valentão, P.; Pereira, J.A.; Andrade, P.B..
In this work, the volatile composition of kale (Brassica oleracea L. var. acephala) and its variation during germination were monitored during the first 9 days of seedling development by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/ion trap-mass spectrometry (GC/IT-MS). Differences were found among the materials in the distinct analyzed periods. A total of 66 volatile compounds, distributed in several chemical classes, were determined: alcohols, carbonyl compounds (ketones, aldehydes, and esters), norisoprenoids, and terpenes, among others, sulfur compounds being the most abundant group in seeds and sprouts that exhibited allyl isothiocyanate as the major compound. Leaves of fully developed ground plant had the highest...
Tipo: Article Palavras-chave: Brassica oleracea L. var. acephala; Kale; Leaves; Sprouts; Seeds; Volatile compounds.
Ano: 2009 URL: http://hdl.handle.net/10198/3118
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Volatile constituents throughout Brassica oleracea L. var. acephala germination. IPB - Escola Superior Agrária
Fernandes, F.; Gomes, D.; Pinho, P. Guedes de; Valentão, P.; Pereira, J.A.; Andrade, P.B..
Brassicaceae family are commonly grown and consumed worldwide. Belonging to this group, kale (Brassica oleraceIJe var. acephala) is important in traditional farming systems in the Iberian Peninsula. While its seeds have been used for human consumption as oil, or mixed with some food products, sprouts, the germination form, are favoured for their nutritional value [1].
Tipo: ConferenceObject
Ano: 2009 URL: http://hdl.handle.net/10198/5139
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Volatile profiling of Ficus carica varieties by HS-SPME and GC-IT-MS. IPB - Escola Superior Agrária
Oliveira, A.P.; Silva, L.R.; Pinho, P. Guedes de; Gil Izquierdo, A.; Valentão, P.; Silva, B.M.; Pereira, J.A.; Andrade, P.B..
Aroma is one of the essential parameters for the evaluation of fruit quality, with volatile components being determinant for this characteristic. During this work, the volatile profile of fresh fruits (pulp and peel) and leaves of Portuguese Ficus carica L. white (‘‘Pingo de Mel” and ‘‘Branca Tradicional”) and dark (‘‘Borrasota Tradicional”, ‘‘Verbera Preta” and ‘‘Preta Tradicional”) varieties were characterised by HSSPME/ GC–IT-MS. Fifty-nine compounds were identified and distributed by distinct chemical classes (aldehydes, alcohols, ketones, esters, monoterpenes, sesquiterpenes, norisoprenoids), with 39 being reported for the first time in this species. Principal component analysis of semi-quantitative data showed that pulps and peels are distinguished...
Tipo: Article Palavras-chave: Ficus carica; Volatile compounds; HS-SPME; GC–IT-MS; PCA.
Ano: 2010 URL: http://hdl.handle.net/10198/3120
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Voracity of coccinellid species on different phenological stages of the olive pest Saissetia oleae (Homoptera, Coccidae) IPB - Escola Superior Agrária
Santos, Sónia A.P.; Pereira, J.A.; Torres, L.; Nogueira, A.J.A..
Coccinellidae are well known predators in agroecosystems. In olive groves they may exert control against scales, such as the black scale, Saissetia oleae (Olivier, 1791). Laboratory studies on the consumption of three phenological stages (eggs, first and second instar nymphs) of S. oleae by four coccinellid species (Chilocorus bipustulatus, Scymnus (Pullus) subvillosus, Scymnus (Mimopullus) mediterraneus and Scymnus (Scymnus) interruptus) were carried out. C. bipustulatus presented a significantly high consumption of eggs, first and second instar nymphs compared with the other species. All coccinellids consumed eggs and first instar nymphs; however the second instar nymphs were only consumed by S. interruptus and C. bipustulatus. In a second experiment,...
Tipo: Article Palavras-chave: Predation; Chilocorus bipustulatus; Pullus (Mimopullus) mediterraneus; Scymnus (Pullus) subvillosus; Scymnus (Scymnus) interruptus; Black scale.
Ano: 2009 URL: http://hdl.handle.net/10198/2596
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Walnut (Juglans regia L.) leaves: phenolic compounds, antimicrobial activity ans antioxidant potential of different cultivars. IPB - Escola Superior Agrária
Pereira, J.A.; Oliveira, I.; Sousa, A.; Valentão, P.; Andrade, P.B.; Ferreira, Isabel C.F.R.; Ferreres, F.; Bento, Albino; Seabra, R.M.; Estevinho, Leticia M..
Different cultivars of walnut (Juglans regia L.) leaves (Cv. Lara, Franquette, Mayette, Marbot, Mellanaise and Parisienne) grown in Portugal, were investigated in what concerns phenolic compounds and antimicrobial and antioxidant properties. Phenolics analysis was performed by reversed-phase HPLC/DAD and 10 compounds were identified and quantified: 3- and 5-caffeoylquinic acids, 3- and 4-pcoumaroylquinic acids, p-coumaric acid, quercetin 3-galactoside, quercetin 3-pentoside derivative, quercetin 3-arabinoside, quercetin 3-xyloside and quercetin 3-rhamnoside. The antimicrobial capacity was screened against Gram positive (Bacillus cereus, B. subtilis, Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella...
Tipo: Article Palavras-chave: Walnut leaves; Phenolics; Antimicrobial activity; Antioxidant properties.
Ano: 2007 URL: http://hdl.handle.net/10198/719
Registros recuperados: 313
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