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Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor BJMBR
Xu,H.H.; Liu,S.H.; Guo,Q.F.; Liu,Q.H.; Li,X.Y..
Bone morphogenetic protein 2 (BMP2) and basic fibroblast growth factor (bFGF) have been shown to exhibit a synergistic effect to promote bone repair and healing. In this study, we constructed a novel adenovirus with high coexpression of BMP2 and bFGF and evaluated its effect on osteogenic differentiation of goat bone marrow progenitor cells (BMPCs). Recombinant adenovirus Ad-BMP2-bFGF was constructed by using the T2A sequence. BMPCs were isolated from goats by density gradient centrifugation and adherent cell culture, and were then infected with Ad-BMP2-bFGF or Ad-BMP2. Expression of BMP2 and bFGF was detected by ELISA, and alkaline phosphatase (ALP) activity was detected by an ALP assay kit. In addition, von Kossa staining and immunocytochemical staining...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BMP2; BFGF; Adenovirus; T2A sequence; BMPCs; Osteogenic differentiation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900809
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Evaluation of lettuce chloroplast and soybean cotyledon as platforms for production of functional bone morphogenetic protein 2. Repositório Alice
QUEIROZ, L. N.; MALDANER, F. R.; MENDES, E. A.; SOUSA, A. R.; D?ALLASTTA, R. C.; MENDONÇA, G.; MENDONÇA, D. B. S.; ARAGÃO, F. J. L..
bitstream/item/195705/1/Queiroz2019-Article-EvaluationOfLettuceChloroplast.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biopharming; BMP2; Lettuce chloroplast; Soybean seeds; Transplastomic plant; Transgenic plants.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108126
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