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*100 Semi-supercentenarians and older as a proposed sample set for the Archon Genomics X PRIZE Validation Protocol*
Provedor de dados: 17
Autores: Thomas Perls; Nir Barzilai.
The &amp;#x22;Archon Genomics X PRIZE Validation Protocol&amp;#x22;:http://precedings.nature.com/documents/5731/version/1 has been posted in part to solicit comments from the scientific community. Here, we suggest 100 subjects age &amp;#x3e;=105 years as the source of DNAs for the competition.&amp;#xd;&amp;#xa;
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5756/version/1

12x coverage 454/Sanger Hybrid Assembly of Cucumber (Cucumis sativus L. cv.Borszczagovski) genome - the most efficient way to begin post genomic era
Provedor de dados: 17
Autores: Rafa&amp;#x142; W&amp;#xf3;ycicki; Justyna Witkowicz; Magdalena Pawe&amp;#x142;kowicz; Ewa Siedlecka; Wojciech Gutman; Wojciech Pl&amp;#x105;der; Anna Seroczy&amp;#x144;ska; Mieczys&amp;#x142;aw &amp;#x15a;miech; Katarzyna Niemirowicz-Szczytt; Stanis&amp;#x142;aw Karpi&amp;#x144;ski; Stefan Malepszy; Zbigniew Przybecki.
New sequencing technologies make it possible to achieve genome sequence fast and cheap. Since the assembly step of such next generation reads is still not well standardized it is the most cumbersome part of sequencing projects. &amp;#xd;&amp;#xa;We present here approaches undertaken on the way to the draft assembly the cucumber (Cucumis sativus L. cv. Borszczagowski) genome done with the use of 8x unpaired and 4x paired (3 Kbp) pyrosequenced 454 XLR Titanium reads, together BAC library ends fragments (12,7x physical coverage). Two different assembly approaches, namely Celera and Celera/Arachne were finally used. The Celera result was 15&amp;#x2019;196 contigs of 197 Mbp length and N50 27&amp;#x2019;086 bp and 4&amp;#x2019;157 scaffolds of 224 Mbp length...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5466/version/1

21 Tesla Micro-MRI of Rat Skin
Provedor de dados: 17
Autores: Rakesh Sharma; Suniket  Fulzele; Kiran Shetty; Mandeep Sachdeva; Bruce R. Locke.
*Aim:* A rapid ultra high resolution ex vivo MRI imaging method was evaluated to visualize rat skin structure. The main objectives were:1.qualitative assessment of viable epidermis, hair, oil and fat-rich skin features by multi-contrast approach;2.achievement of spatial resolution up to 15 microns; 3.comparison of skin histology with MRI skin features obtained by 21 Tesla magnetic resonance imaging (MRI) imager. &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;*Introduction:* The ultrawide bore 900 MHz magnet has high resolution NMR magnet has a central field of 21.1 T. The high resolution MRI technique has potential for both quantitative and non-invasive morphological evaluation at micrometer level. The MRI signal was sensitive to water protons and glycolipid...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2639/version/1

21 Tesla MRI Microimaging of Rat Heart by Superparamagnetic Antimyoglobin Bound Nanoparticles
Provedor de dados: 17
Autores: Rakesh Sharma.
Superparamagnetic iron-oxide bound antimyoglobin (SPIOM) characteristics were analyzed at different pH.  After injecting SPIOM in heart, ex vivo magnetic resonance microimaging (MRM) technique was used to visualize microvasculature of rat heart including cardiac arteries, veins, cordate tendons attached with valves.  The measurement accuracy of ventricles, aorta, vasculature and dimensions of cordate tendons were close to coregistered histology measurements.
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2643/version/2

21 Tesla MRI Microimaging of Rat Heart by Superparamagnetic Antimyoglobin Bound Nanoparticles
Provedor de dados: 17
Autores: Rakesh Sharma.
Superparamagnetic iron-oxide bound antimyoglobin (SPIOM) characteristics were analyzed at different pH.  After injecting SPIOM in heart, ex vivo magnetic resonance microimaging (MRM) technique was used to visualize microvasculature of rat heart including cardiac arteries, veins, cordate tendons attached with valves.  The measurement accuracy of ventricles, aorta, vasculature and dimensions of cordate tendons were close to coregistered histology measurements.
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2643/version/1

21 Tesla MRI Microscopy of Mice Kidney, Heart and Skin: Quantitation of MRI Visible Features
Provedor de dados: 17
Autores: S Sharma; Rakesh Sharma.
Excised mice kidney, heart and skin MRI visible features were quantitated using ex vivo 21 Tesla MRI microimager. The fast MRI acquisition protocols of FLASH, gradient echo flow compensated (GEFC) techniques were used to visualize microvasculature of mice kidney, mice heart including cardiac arteries, veins, cordate tendons and mice skin epidermis with hair follicle features.
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3484/version/1

21 Tesla Rat Heart Magnetic Resonance Microimaging by Paramagnetic Anti-Troponin Bound Polyethylene Based Iron-Oxide Nanoparticles and Image Processing
Provedor de dados: 17
Autores: Rakesh Sharma.
21T MR microimaging with the first use of troponin nanoparticles enhances the visualization of cardiac muscle fibers and offers technical advancement for the future. Diffusion weighting offers fiber tracking and functional analysis. Image processing offers heart probabilistic atlas and maps.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;http://www.ismrm.org/10/program/ELECTRONICPOSTERS.htm
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6650/version/1

A Bacterial Enzyme Catalyzing Double Reduction of a &amp;#x3b2;,&amp;#x3b4;-Diketo Ester with Unprecedented Stereoselectivity
Provedor de dados: 17
Autores: Xuri Wu; Nan Liu; Yunmian He; Yijun Chen.
Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/1697/version/1

A Bacterial Enzyme Catalyzing Double Reduction of a &amp;#x3b2;,&amp;#x3b4;-Diketo Ester with Unprecedented Stereoselectivity
Provedor de dados: 17
Autores: Xuri Wu; Nan Liu; Yunmian He; Yijun Chen.
Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2010
URL: http://precedings.nature.com/documents/1697/version/2

A Bibliometric Analysis of Microalgae Research in the World, Europe, and the European Atlantic Area
Provedor de dados: 5
Autores: Rumin, Judith; Nicolau, Elodie; Gonçalves De Oliveira Junior, Raimundo; Fuentes-grünewald, Claudio; Flynn, Kevin J; Picot, Laurent.
A bibliographic database of scientific papers published by authors affiliated worldwide, especially focused in Europe and in the European Atlantic Area, and containing the keywords “microalga(e)” or “phytoplankton” was built. A corpus of 79,020 publications was obtained and analyzed using the Orbit Intellixir software to highlight the evolution of the research domain. Publication rates from 1960 to 2019, organization of the research, collaboration networks between countries and organizations, emerging and fading research concepts, major studied species, and associated concepts, as well as journals publishing microalgae research were considered. As a result, of the 79,020 papers published worldwide, 26,137 included authors from Europe (33% of world...
Tipo: Text
Palavras-chave: Biotechnology; European Atlantic Area; Microalgae; Phytoplankton; Research; Market; Bibliometrics.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00605/71725/70191.pdf

A call for disruptive innovation in science publishing with a new open data-sharing platform for the life sciences
Provedor de dados: 17
Autores: Donald  C. Cooper.
&amp;#x201c;A disruptive innovation is an innovation that helps create a new market and value network, and eventually goes on to disrupt an existing market and value network (over a few years or decades), displacing an earlier technology. The term is used in business and technology literature to describe innovations that improve a product or service in ways that the market does not expect.&amp;#x201d; -Wikipedia&amp;#xd;&amp;#xa;On April 3rd, 2012 Nature Precedings, Nature Publishing Group&amp;#x2019;s experiment in free pre-print publishing was shut down and no longer accepts submissions. According to the Nature Precedings website it was created in 2007 as &amp;#x201c;a place for researchers to share documents, including presentations, posters, white...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7151/version/1

A comparative study on the production of ethanol from lignocellulosic biomass by chemical and biological method
Provedor de dados: 17
Autores: Shaheed SM; Rohit JP; Madhan R; Selvakumar K.
Ethanol derived from non-edible biomass is renewable and a clean source of energy. It is independent of the food industry and it is economically feasible. The first generation biofuel or bioethanol is still not a very convenient source of energy as it prominently depends on the availability of grains. The main objective of this work is to develop an industrious efficient process to produce ethanol from lignocellulosic biomasses like wood and leaf in a lab scale. Two processes were compared. The first process involved an alkaline pre-treatment of the powdered biomass followed by dilute acid hydrolysis. The second process involved an alkaline treatment followed by direct hydrolysis of the biomass by use of a fungal species obtained from rotting wood....
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6579/version/1

A conceptual and practical overview of cDNA microarray technology: implications for basic and clinical sciences
Provedor de dados: 56
Autores: Mello-Coelho,V. de; Hess,K.L.
cDNA microarray is an innovative technology that facilitates the analysis of the expression of thousands of genes simultaneously. The utilization of this methodology, which is rapidly evolving, requires a combination of expertise from the biological, mathematical and statistical sciences. In this review, we attempt to provide an overview of the principles of cDNA microarray technology, the practical concerns of the analytical processing of the data obtained, the correlation of this methodology with other data analysis methods such as immunohistochemistry in tissue microarrays, and the cDNA microarray application in distinct areas of the basic and clinical sciences.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; CDNA microarray; Gene expression; Genomics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000011

A Critical Evaluation of Clinical Trials in Cancer and Pharmacogenomics
Provedor de dados: 17
Autores: I. C. Baianu.
A critical overview of recent clinical trials in cancer is presented focused on signaling pathways blockers or inhibitors with a view to developing successful clinical trials employing personalized cancer therapies. Rational, pharmacogenomic strategies in cancer trials should be adopted that include specific molecular targeting based on adequate data for, and detailed modeling of, cancer cell genomes, modifications of cancer signaling pathways and epigenetic mechanisms. Novel translational oncogenomics research is rapidly expanding through the application of highly sensitive and specific advanced technology, research findings and computational tools and complex models to both pharmaceutical and clinical problems. Multiple sample analyses from several...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7052/version/1

A database of naturally occurring human urinary peptides and proteins for use in clinical applications
Provedor de dados: 17
Autores: Petra Z&amp;#xfc;rbig; Joshua Coon; Hartwig Bauer; Georg Behrens; Mohammed Dakna; Anna Dominiczak; Stephane Decramer; Jochen Ehrich; Danilo Fliser; Moritz Frommberger; Arnold Ganser; Mark Giolami; Igor Golovko; David Good; Wilfried Gwinner; Marion Haubitz; Stefan Herget-Rosenthal; Holger Jahn; George Jerums; Bruce Julian; Markus Kellmann; Volker Kliem; Walter Kolch; Andrzej Krolewski; Mario Luppi; Ziad Massy; Michael Melter; Christian Neus&amp;#xfc;ss; Jan Novak; Karlheinz Peter; Kasper Rossing; Harald Rupprecht; Joost Schanstra; Eric Schiffer; Jens-Uwe Stolzenburg; Lise Tarnow; Dan Theodorescu; Visith Thongboonkerd; Raymond Vanholder; Eva Weissinger; Harald Mischak; Philippe Schmitt-Kopplin.
Owing to its availability, ease of collection and correlation with (patho-) physiology, urine is an attractive source for clinical proteomics. However, the lack of comparable datasets from large cohorts has greatly hindered development in this field. Here we report the establishment of a high resolution proteome database of naturally occurring human urinary peptides and proteins - ranging from 800-17,000 Da - from over 3,600 individual samples using capillary electrophoresis coupled to mass spectrometry, yielding an average of 1,500 peptides per sample. All processed data were deposited in an SQL database, currently containing 5,010 relevant unique urinary peptides that serve as classifiers for diagnosis and monitoring of diseases, including kidney and...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1219/version/1

A Dynamic Decision Model of Technology Adoption under Uncertainty: Case of Herbicide-Resistant Rice
Provedor de dados: 31
Autores: Annou,   Mamane Malam; Wailes,   Eric J.; Thomsen,   Michael R.
Herbicide-resistant (HR) rice technology is a potential tool for control of red rice in commercial rice production.  Using an ex ante mathematical programming framework, this research presents an empirical analysis of HR rice technology adoption under uncertainty.  The analysis accounts for stochastic germination of red rice and sheath blight to model a profit maximization problem of crop rotation among HR rice, regular rice, and soybeans.  The results demonstrate that risk attitudes and technology efficiency determine adoption rates and optimal rotation patterns.
Tipo: Journal Article
Palavras-chave: Biotechnology; Herbicide resistance; Mathematical programming; Profit maximization; Rice; Risk; Rotation; Technology; Adoption; Crop Production/Industries; Production Economics; Research and Development/Tech Change/Emerging Technologies; Q16; Q18; O33; C61.
Ano: 2005
URL: http://purl.umn.edu/43724

A gap analysis of confined field trial application forms for genetically modified crops in East Africa: evaluating the potential for harmonization
Provedor de dados: 31
Autores: Linacre,   Nicholas A.; Cohen,   Joel I.
The regulatory approval of genetically modified crops in the field initially requires small, restricted experimental trials known as confined field trials. These small scale experiments provide researchers with important information on environmental interactions and agronomic performance of the crop in a safe and contained manner. To authorize confined field trials regulatory review is required, with formats for obtaining relevant information differing from country to country. In this paper, a Gap Analysis is used to identify informational gaps and potential for harmonization of confined field trial application processes in three East African countries - Tanzania, Kenya, and Uganda. The basic principle behind gap analysis is a comparison of the status quo...
Tipo: Report
Palavras-chave: Genetically modified crops; Gap analysis; Confined field trials; Biotechnology; Biosafety; Crop Production/Industries.
Ano: 2006
URL: http://purl.umn.edu/55421

A Meta-Analysis of Genetically Modified Food Valuation Studies
Provedor de dados: 31
Autores: Lusk,   Jayson L.; Jamal,   Mustafa; Kurlander,   Lauren; Roucan,   Maud; Taulman,   Lesley.
A plethora of research in recent years has been devoted to estimating consumer demand for genetically modified food, an important piece of information needed to create appropriate public policy. To examine this body of work, a meta-analysis was conducted of 25 studies that, in aggregate, report 57 valuations for GM food. Findings indicate as much as 89% of the variation in existing value estimates for genetically modified food can be explained by an econometric model that controls for (a) the characteristics of the sample of consumers studied, (b) the method for eliciting consumers' valuation, and (c) characteristics of the food being valued. Each of these factors has a statistically significant effect on estimated premiums for non-GM food.  Results of...
Tipo: Journal Article
Palavras-chave: Biotechnology; Consumer acceptance; Genetically modified food; Willingness to pay; Consumer/Household Economics.
Ano: 2005
URL: http://purl.umn.edu/30782

A Micropropagation, Molecular and Physiochemical Analysis of Canola Brassica napus L. From Seeds
Provedor de dados: 17
Autores: Israr  Khan; Muhammad Shuaib; Sikandar Khan; Muhammad Amir Zia; Haji Akbar.
Canola is important for its oil, anticarcinogenic phytochemicals and low saturated fat content and has wide scope for further improvement through genetic transformations. Micropropagation is the practice of rapidly multiplying stock plant material to produce a large number of disease free progeny plants, using modern asceptic plant tissue culture methods and also to conserve germ plasm. A combination of auxin and cytikinin and a shock on growth regulator free medium is used for micropropagation. Molecular and physiochemical analysis done to know variation in seeds protein content and electric conductivity.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4781/version/2

A MODEL OF PESTICIDE RESISTANCE AS A COMMON PROPERTY AND EXHAUSTIBLE RESOURCE
Provedor de dados: 31
Autores: Secchi,   Silvia; Babcock,   Bruce A.
A dynamic farm production model analyzes the interaction between the externalities caused by pest mobility and the development of pesticide resistance, a nonrenewable resource, in the context of agricultural biotechnologies.  The model measures the effect of farmers' myopic behavior and the impact of pest mobility on the path of resistance.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Common property resource; European Corn Borer; Externalities; Pesticide resistance; Intertemporal optimization; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/21664

A new outlook towards kidney injuries
Provedor de dados: 17
Autores: Mahesh Satwekar; Abhijeet Satwekar.
Acute and chronic progression of injury to the kidney leads to the failure of the renal system and has become an increasingly important cause of morbidity and mortality. Present diagnosis detects the condition only after irreversible loss of 70 percent of kidney function. Current research is focused only on the clinical manifestations after the kidney injuries and not towards the exact cause of the condition. Here we propose a new outlook- that there is an involvement of a pathogen in the pathogenesis of kidney injuries. Basis for our proposal is given by the similarity of the pathogenesis events occurring between a classical example of hepatitis and kidney injuries. Furthermore, literature regarding the role of early kidney injury biomarkers in innate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology.
Ano: 2012
URL: http://precedings.nature.com/documents/7021/version/1

A new potential  radiosensitizer: ammonium persulfate modified WCNTs
Provedor de dados: 17
Autores: Jian-She Yang; Bo-Zhang Yu; Wen-Xin Li.
Radiotherapy plays a very important role in cancer treatment. Radiosensitizers have been widely used to enhance the radiosensitivity of cancer cells at given radiations. Here we fabricate multi-walled carbon nanotubes with ammonium persulfate, and get very short samples with 30-50 nanometer length. Cell viability assay show that f-WCNTs induce cell death significantly. We hypothesize that free radicals originated from hydroxyl and carbonyl groups on the surface of f-WCNTs lead cell damage.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2007
URL: http://precedings.nature.com/documents/1421/version/1

A novel class of endogenous shRNAs in human cells
Provedor de dados: 17
Autores: Tongjun Gu; James Q. Yin; Yanwei Xu; ZhengHua Dai; Zhengge Qiu; Shenzhong Feng; Xiang Yi; Ling Jiang; Hongjie Zhang.
Until now it is still not clear how many types and amounts of small RNAs (sRNAs) exist in humans. Here we report the identification of 1258 distinct sRNAs derived from intronic regions of protein-coding genes in human with a new approach. These endogenous short hairpin RNAs (shRNAs) appear to be similar to exogenous shRNAs in structure, have a broad distribution in the stem length, and function as microRNAs (miRNAs), small interfering RNAs (siRNA) and/or piwi-interacting RNAs (piRNAs). Except for a few shRNAs, the majority of shRNAs are not phylogenetically conserved. They are differentially expressed in different cells and at diverse developmental stages. Overall, their expression levels are lower than miRNAs&amp;#x27;, but can be detected by quantitative...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1531/version/1

A novel mathematical tool for generating highly conserved protein domain via different organismal genomic landscapes
Provedor de dados: 17
Autores: Arunava Goswami; Pabitra Pal Choudhury; Rajneesh Singh; Sk. Sarif Hassan.
Darwinian evolution hypothesizes that a short stretch of DNA was first constructed and then it expanded to give rise to a long strand. This long strand then produced a mix of exons, introns and repetitive DNA sequence. The order of production of above three kinds of DNA sequence is unknown. Reshuffling of stretches of DNA like above within organisms has given rise to different chromosomes. Till date it is not known how this process is governed. In this paper we show that starting with a sixteen base-pair human olfactory DNA sequence one can form a highly conserved protein domain. Once this domain is formed repetitive DNA sequences of a particular kind starts generating which signifies that this particular conserved protein domain will be unique in nature....
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4850/version/1

A Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for  Composition Improvements-  (v.4)
Provedor de dados: 17
Autores: Valentin  Prisecaru; I. C. Baianu.
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions.  Conventional &amp;#x201c;wet chemistry&amp;#x201d; analytical methods are time-consuming and costly.  As a result, soybean breeders and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive method for composition analysis for academia and industry.  Recent advancements in instrumentation design, such as the application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly improved overall instrument...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Bioinformatics; Earth &amp; Environment; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6231/version/4

A novel strategy for the targeted analysis of protein and peptide metabolites
Provedor de dados: 17
Autores: Nicholas A. Williamson; Charles Reilly; Chor-Teck Tan; Sri-Harsha Ramarathinam; Alun Jones; Christie L. Hunter; Francis R. Rooney; Anthony W. Purcell.
The detection and quantitation of exogenously administered biological macromolecules (e.g. vaccines, peptide and protein therapeutics) and their metabolites is frequently complicated by the presence of a complex endogenous mixture of closely related compounds. We describe a method that incorporates stable isotope labeling of the compound of interest allowing the selective screening of the intact molecule and all metabolites using a modified precursor ion scan. This method involves monitoring the low molecular weight fragment ions produced during MS/MS that distinguish isotopically labelled material from related endogenous compounds. All isotopically labelled substances can be selected using this scanning technique for further analysis whilst other...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Pharmacology.
Ano: 2009
URL: http://precedings.nature.com/documents/3812/version/1

A Paradigm for Biology&amp;#x27;s Next Revolution
Provedor de dados: 17
Autores: Fredric D. R. Young.
A recent essay on biology&amp;#x27;s next revolution described attributes of a future theory of biology that would replace post hoc modeling with an interplay between quantitative prediction and experimental test, more characteristic of the physical sciences1. The article suggested that flux from the environment whether it be energy, chemicals metabolites, or genes is a defining characteristic of life. The essay also suggests that an interdisciplinary approach to collective phenomena centered on statistical mechanics and dynamical systems theory will be required to make use of this perspective. Over the last 30 years we have developed an approach to drug discovery and translational research that is based on a paradigm for complex systems modeling that fits...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1770/version/1

A potential role for Dkk-1 in the pathogenesis of osteosarcoma predicts novel diagnostic and treatment strategies.
Provedor de dados: 17
Autores: Carl Gregory; Narae Lee; Angela Smolarz; Scott Olson; Odile David; Jacob Reiser; Robert Kutner; Najat Daw; Darwin Prockop; Edwin Horwitz.
Canonical Wnt signaling is an osteo-inductive signal that promotes bone repair through acceleration of osteogenic differentiation by progenitors. Dkk-1 is a secreted inhibitor of canonical Wnt signaling and thus inhibits osteogenesis. To examine a potential osteo-inhibitory role of Dkk-1 in osteosarcoma (OS), we measured serum Dkk-1 in pediatric patients with OS (median age, 13.4 years) and found it to be significantly elevated. We also found that Dkk-1 was maximally expressed by the OS cells at the tumor periphery and _in vitro_ Dkk-1 and RANKL are co-expressed by rapidly proliferating OS cells. Both Dkk-1 and conditioned media from OS cells reduces osteogenesis by human mesenchymal cells and by immuno-depletion of Dkk-1, or by adding a GSK3[beta]...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer.
Ano: 2007
URL: http://precedings.nature.com/documents/130/version/1

A prelude report on molecular docking of HER2 protein towards comprehending anti-cancer properties of saponins from Solanum tuberosum
Provedor de dados: 17
Autores: Puneet  K. Singh; Pratyoosh Shukla.
Saponins are extensively known for many biological activities e.g. antimicrobial, anti-palatability, anti-cancer and hemolytic. As cancer cells have a more cholesterol-like compound in their membrane structure the saponins bind cholesterol due to their natural affinity to bind cancer cell membrane. This prevents them from entering the body through the intestinal tract, where they have the ability to attach themselves to vital organs and grow. This study reports the effective use of lower dose saponins like immunotoxin so that they can inhibit the proliferation of cancerous pancreatic cells. The investigation of pancreatic cancer metabolic pathway it was found that proteins 3H3B produced by genes HER-2 are involved in the enhancement of this type of cancer....
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7147/version/1

A Proposal of Genomic Analytical Workflow in a Bacterial Pathogen Outbreak Investigation
Provedor de dados: 17
Autores: Hoi Shan Kwan; Chun Hang Au; Chi Keung Cheng; Man Kit Cheung; Qianli Huang; Lei Li; Wenyan Nong; Man Chun Wong.
The German Escherichia coli O104:H4 outbreak causing Hemolytic Uremic Syndrome (HUS) started in early May 2011. Conventional typing methods including serotyping, multi-locus sequence typing (MLST) and pulse-field gel electrophoresis (PFGE) seemed to yield only limited information about the causative agent. The advantages of the Genome sequencing approach are apparent compared to conventional methods. Genome sequencing and analyses with an appropriate workflow would be the new paradigm in bacterial outbreak investigation.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology.
Ano: 2011
URL: http://precedings.nature.com/documents/6663/version/1

A quantitative model for efficient construction of lentiviral vectors with a unique clone site
Provedor de dados: 17
Autores: Gang Zhang; Anurag  Tandon.
Lentiviral vectors (LVs) were a powerful tool for transgene expression in vivo and in vitro. However, the construction of LVs is of low efficiency, due to the large sizes and lack of proper clone sites. Therefore, it is critical to develop efficient strategies for cloning LVs. Here, we reported a combinatorial strategy to efficiently construct LVs using EGFP, hPlk2 wild type (WT) and mutant genes as inserts. Firstly, site-directed mutagenesis (SDM) was performed to create BamH I site for the inserts; secondly, pWPI LV was dephosphorylated after BamH I digestion; finally, the amounts and ratios of the insert and vector DNA were optimized to increase monomeric ligation. Our results showed that the total percentage of positive clones was approximately...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6876/version/1

A Reaction Route Approach to Flux Balance Analysis
Provedor de dados: 17
Autores: Ilie  Fishtik.
Background: Flux balance and network-based pathway analyses are theoretical tools aimed to find optimal steady state flux distributions in a metabolic network subject to additional constraints on the rates of the reaction steps. Although these methods are mathematically accurate, there are several physicochemical and computational aspects that are questionable and misleading. In particular, it is well known that the flux balance analysis may result in multiple flux distributions for the same objective function.  &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;Results: The flux balance and network-based pathway analyses are reformulated in terms of reaction routes (RRs), a theoretical framework that has been developed by Horiuti over 50 years ago. Not only does the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/2788/version/1

A Simple, Rapid Method for Extracting Large Plasmid DNA from Bacteria
Provedor de dados: 17
Autores: Spencer D. Heringa; Jonathan D. Monroe; James B. Herrick.
We are studying the lateral transfer of transmissible antibiotic resistance plasmids among stream bacteria impacted by fecal runoff from poultry and cattle. Such plasmids are typically large (ca. 40 &amp;#x2013; 100 kb) and occur in low copy numbers in the cell and have therefore typically been difficult to isolate and therefore to study. Traditional protocols, based upon variations of the standard alkaline-lysis method, are long (ca. 1 1/2 to 2 days) and difficult. Commercial kits designed for the isolation of Baterial Artificial Chromosomes (BACs) can be used and are an improvement; however, these are expensive and still require hours of sustained effort. We have adapted a method published by Rondon et al. (1999), originally designed for the isolation of...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology.
Ano: 2007
URL: http://precedings.nature.com/documents/1249/version/1

A subset of co-expressed genes in Slug-based cancer mesenchymal transition signature remains coexpressed in normal samples in a tissue-specific manner
Provedor de dados: 17
Autores: Weiyi Cheng; Dimitris Anastassiou.
A recently identified gene expression signature of EMT markers containing the transcription factor Slug was found present in samples from many publicly available cancer gene expression datasets of multiple cancer types except leukemia. We also found many of these genes co-expressed in human cancer xenografted cells, but not in mouse stroma cells, suggesting that the signature is largely produced by cancer cells undergoing some type of EMT. Here we report that a partial signature consisting of a subset of the co-expressed genes of the full signature, including at least Slug (SNAI2), collagens COL1A1, COL1A2, COL3A1, COL6A3 and genes DCN and LUM, is also present in leukemia, in which case it is also strongly associated with the chemokine CXCL12 (aka SDF1)....
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6813/version/1

A Systematic Approach to Identifying Protein-Ligand Binding Profiles on a Proteome Scale
Provedor de dados: 17
Autores: Li Xie; Nancy  Buchmeier; Lei Xie; Philip Bourne.
Identification of protein-ligand interaction networks on a proteome scale is crucial to address a wide range of biological problems such as correlating molecular functions to physiological processes and designing safe and efficient therapeutics. We have developed a novel computational strategy to identify ligand binding profiles of proteins across gene families and applied it to predicting protein functions, elucidating molecular mechanisms of drug adverse effects, and repositioning safe pharmaceuticals to treat different diseases.
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2207/version/1

A Targeted Therapeutic Rescues Botulinum Toxin-A Poisoned Neurons
Provedor de dados: 17
Autores: Prabhati Ray; Bal Ram Singh; Radharaman Ray; Peng Zhang; Dan Li; James Keller; Michael Adler.
Botulinum neurotoxin (BoNT), a Category A biothreat agent, is the most potent poison known to mankind. Currently no antidote is available to rescue poisoned synapses. BoNT acts specifically by blocking neurotransmission primarily at peripheral nerve-muscle junctions causing severe flaccid muscle paralysis, which is fatal if proper medical care is not provided. The neurotoxin acts by specifically entering the presynaptic nerve endings where it interferes with the biochemical machinery involved in the process of neurotransmitter release, i.e., neuroexocytosis. Most serotypes of BoNT are known to remain active for weeks to months after entering the nerves, but BoNT/A is the most potent and long lasting in causing muscle paralysis. An effective medical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2149/version/1

A Three dimesional stochastic spatio-temporal model of cell spreading
Provedor de dados: 17
Autores: Yuguang P. Xiong; Padmini Rangamani; Benjamin Dubin-Thaler; Michael  Sheetz; Ravi Iyengar.
Cell motility is important for many physiological processes and the underlying&amp;#xd;&amp;#xa;biochemical reactions motility have been well-characterized. Mathematical models, using&amp;#xd;&amp;#xa;the biochemical reactions and focused on different types of spreading behavior have been&amp;#xd;&amp;#xa;constructed and analyzed. In this study, we build on these previous models to develop a&amp;#xd;&amp;#xa;three-dimensional stochastic model of isotropic spreading of mammalian fibroblasts. The model is composed of three actin remodeling reactions that occur stochastically in space and time and are regulated by membrane resistance forces. Numerical simulations indicate that the model qualitatively captures the experimentally observed isotropic cell...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/62/version/1

A three-dimensional stochastic spatio-temporal model of cell spreading
Provedor de dados: 17
Autores: Yuguang P. Xiong; Padmini Rangamani; Benjamin Dubin-Thaler; Michael  Sheetz; Ravi Iyengar.
Cell motility is important for many physiological processes and the underlying&amp;#xd;&amp;#xa;biochemical reactions motility have been well characterized. Mathematical models, using&amp;#xd;&amp;#xa;the biochemical reactions and focused on different types of spreading behavior have been&amp;#xd;&amp;#xa;constructed and analyzed. In this study, we build on these previous models to develop a&amp;#xd;&amp;#xa;three-dimensional stochastic model of isotropic spreading of mammalian fibroblasts. The model is composed of three actin remodeling reactions that occur stochastically in space and time and are regulated by membrane resistance forces. Numerical simulations indicate that the model qualitatively captures the experimentally observed isotropic cell...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/62/version/2

A unique avenue to control drug resistant pathogens: Experimental evidences
Provedor de dados: 17
Autores: Sribir Sen.
Drug resistant bacterial pathogens are increasing in the environment due to multiple reasons. If any drug resistant pathogen grows in a host, the host will face death treat because there is no way to resist their growth in the host system. Eventuality is death in almost all cases. Reports on the mechanisms of drug resistance in bacteria are many. However, there is no solution for these cases. The present work is providing an important understanding to fight against drug resistant pathogens and that can be used in gastrointestinal diseases.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4934/version/1

A Universal Carrier Test for the Long Tail of Mendelian Disease
Provedor de dados: 17
Autores: Balaji S. Srinivasan; Jason Flannick; A. Scott Patterson; Christopher C. Chang; Tuan Pham; Sharon Young; Amit Kaushal; James J. Lee; Pasquale Patrizio; Eric A. Evans.
*Background:* &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;Mendelian disorders are individually rare but collectively common, forming a &amp;#x22;long tail&amp;#x22; of genetic disease. More than 20 million people worldwide suffer from a disease in this long tail before the age of 25, with minorities and developing countries at highest risk and with the number of carriers far in excess of this figure. Importantly, the Jewish community&amp;#x2019;s campaign for universal Tay-Sachs screening shows that these incurable diseases can nevertheless be prevented if carrier status is known before conception. A single highly-accurate assay for the long tail of Mendelian disease would allow us to scale this successful campaign up to the general population, thereby improving...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4192/version/1

Accommodating the difference in students' prior knowledge of cell growth kinetics
Provedor de dados: 69
Autores: Seters,Janneke van; Ossevoort,Miriam; Goedhart,Martin; Tramper,Johannes.
This paper describes the development and benefits of an adaptive digital module on cell growth to tackle the problem of educating a heterogeneous group of students at the beginning of an undergraduate course on process engineering. Aim of the digital module is to provide students with the minimal level of knowledge on cell growth kinetics they need to comprehend the content knowledge of the subsequent lectures and pass the exam. The module was organised to offer the subject matter in a differentiated manner, so that students could follow different learning paths. Two student groups were investigated, one consisting of students who had received their prior education abroad and one of students that had not. Exam scores, questionnaires, and logged user data...
Tipo: Journal article
Palavras-chave: Adaptive digital learning material; Bioreactor design; Biotechnology; Education; Intelligent tutoring system; Intelligent Web-based educational system.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200001

Acute Toxicity of Perfluorooctane Sulfonic Acid on Early Life Stages of Daphnia Magna, Siriella Armata, Paracentrotus Lividus Sea Urchin, and Turbot (Scophthalmus Maximus L.).
Provedor de dados: 17
Autores: Lazhar Mhadhbi; Sara P&amp;#xe9;rez Fernandez; Diego Real Conde; Moncef Boumaiza; Ricardo Beiras.
Perfluorooctane sulfonate (PFOS) is a persistent organic pollutant whose potential toxicity is causing great concern. In the present study, we used two crustaceans (_Daphnia magna_, _Siriella armata_), echinoid embryos (_Paracentrotus lividus_) and turbot (_Scophthalmus maximus L._) embryos and larvae to investigate the acute toxicity of this compound. The marine species _S. armata_ exhibited greater sensitivity than the freshwater species _D. magna_. In the 48-h acute toxicity test the median lethal concentration was 10.96 mg/L for _S. armata_ and 87.46 mg/L for _D. magna_. In the 96h toxicity test, turbot showed the lowest EC50 (0.155 mgL-1), whilst the EC50 for _S. armata_ was 7.92 mgL-1 which was approximately three times lower than that for sea urchin...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Ecology; Earth &amp; Environment.
Ano: 2010
URL: http://precedings.nature.com/documents/4657/version/1

Adding automated data analysis and biological evaluation to affyanalysisQC
Provedor de dados: 17
Autores: Anwesha Dutta.
Almost every cell in the body contains a full set of chromosomes and identical genes. However, only a fraction of these genes are turned on, at any given time and it is this subset of genes that are expressed, which confers unique properties to each cell type.  Gene expression is the term used to describe the transcription of the information contained within the DNA into messenger RNA (mRNA) molecules that are then translated into proteins which perform most of the critical functions of cells. &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;Gene expression is a highly complex and tightly regulated process that allows a cell to respond dynamically both to environmental stimuli and to its own changing needs. This mechanism acts as both an on/off switch to control which...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5969/version/1

ADOPTION OF BIOENGINEERED CROPS
Provedor de dados: 31
Autores: Fernandez-Cornejo,   Jorge; McBride,   William D.
Use of crop biotechnology products, such as genetically engineered (GE) crops with input traits for pest management, has risen dramatically since commercial approval in the mid-1990s. This report addresses several of the economic dimensions regarding farmer adoption of bioengineered crops, including herbicidetolerant and insect-resistant varieties. In particular, the report examines: (1) the extent of adoption of bioengineered crops, their diffusion path, and expected adoption rates over the next few years; (2) factors affecting the adoption of bioengineered crops; and (3) farm-level impacts of the adoption of bioengineered crops. Data used in the analysis are mostly from USDA surveys.
Tipo: Report
Palavras-chave: Biotechnology; Technology adoption; Genetic engineering; Pest management; Financial effects; Tillage; Herbicide-tolerant crops; Bt crops; Corn; Soybeans; Cotton; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/33957

Adoption of Transgenic Crops by Smallholder Farmers in Entre Rios, Argentina
Provedor de dados: 31
Autores: Paredes,   Cecilia; Martin,   Marshall A.
This is a study of the adoption of transgenic crops by 120 smallholder farmers interviewed in July 2005 in the communities of San Jose de Feliciano and La Paz in the Entre Rios Province of Argentina. Logistic regression results indicate that access to a planter is essential for smallholders to adopt Bt corn, while adopters of Roundup Ready™ soybeans have larger farms, access to credit, availability of all essential machinery, more years of schooling, and are primarily located in the La Paz community.
Tipo: Conference Paper or Presentation
Palavras-chave: Transgenic crops; Technology adoption; Bt corn; Roundup Ready™ soybeans; Biotechnology; Argentina; Smallholders; Farm Management; Research and Development/Tech Change/Emerging Technologies.
Ano: 2007
URL: http://purl.umn.edu/9996

Advancing transcriptome platforms
Provedor de dados: 17
Autores: Shuobo Shi.
During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of the transcriptome at unprecedented scale and speed. Studies using these methods have already altered our view of the extent and complexity of transcript profiling, which has advanced from one-gene-at-a-time to a holistic view of the genome. Here, we outline the major technical advances in transcriptome characterization, including the most popular used hybridization-based platform, the well accepted tag-based sequencing platform, and the recently developed RNA-Seq (RNA sequencing) based platform. Importantly, these next-generation technologies revolutionize assessing the entire transcriptome via the recent RNA-Seq technology.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5894/version/1

Affymetrix probes containing runs of contiguous guanines are not gene-specific
Provedor de dados: 17
Autores: Graham J. Upton; William B. Langdon; Andrew P. Harrison.
High Density Oligonucleotide arrays (HDONAs), such as the Affymetrix HG-U133A GeneChip, use sets of probes chosen to match specified genes, with the expectation that if a particular gene is highly expressed then all the probes in the designated probe set will provide a consistent message signifying the gene&amp;#x27;s presence. However, we demonstrate by data mining thousands of CEL files from NCBI&amp;#x27;s GEO database that 4G-probes (defined as probes containing sequences of four or more consecutive guanine (G) bases) do not react in the intended way. Rather, possibly due to the formation of G-quadruplexes, most 4G-probes are correlated, irrespective of the expression of the thousands of genes for which they were separately intended. It follows that...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1825/version/1

Aggregation of Single Nucleotide Polymorphisms in a Human H5N1 Clade 2.2 Hemagglutinin
Provedor de dados: 17
Autores: Henry L. Niman; Magdi D. Saad; Jeffery Tjaden; Kenneth C. Earhart; Marshall R. Monteville; Mona M. Aly; Moustafa M. Mansour; Nasr El-Sayed; Ahmed E. Nayel; Ahmed S. Abdelghani; Hala M. Esmat; Emad M. Labib; Ehab A. Ayoub; Abdelattar Arafa; Gregory A. Raczniak; Mensah Agyen-Frempong; William K. Ampofo; Bruce R. Boynton.
The evolution of H5N1 has attracted significant interest 1-4 due to linkages with avian 5,6 and human infections 7,8.  The basic tenets of influenza genetics 9 attribute genetic drift to replication errors caused by a polymerase complex that lacks a proof reading function.  However, recent analysis 10 of swine influenza genes identifies regions copied with absolute fidelity for more than 25 years.  In addition, polymorphism tracing of clade 2.2 H5N1 single nucleotide polymorphisms identify concurrent acquisition 11 of the same polymorphism onto multiple genetic backgrounds in widely dispersed geographical locations.  Here we show the aggregation of regional clade 2.2 polymorphisms from Germany, Egypt, and sub-Sahara Africa onto a human Nigerian H5N1...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/743/version/1

Agricultural Biotechnology and the FTAA: Issues and Opportunities
Provedor de dados: 31
Autores: Gaisford,   James D.
This article examines trade and intellectual property rights questions associated with agricultural biotechnology in the Western Hemisphere and goes on to chart a potential course through which they could be addressed by an agreement on a Free Trade Area of the Americas. Issues pertaining to import approvals, labelling, exports to sensitive markets, intellectual property piracy and regulatory cooperation are considered.
Tipo: Journal Article
Palavras-chave: Biotechnology; BioSafety Protocol; Free Trade Area of the Americas; FTAA; World Trade Organization; International Relations/Trade; Research and Development/Tech Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/23926

Agricultural Resources and Environmental Indicators, 2006 Edition
Provedor de dados: 31
Autores: Wiebe,   Keith D.; Gollehon,   Noel R.
This is a large file. We have had reports of problems opening the file in Mozilla Firefox. If you have problems, try using Windows Internet Explorer.
Tipo: Report
Palavras-chave: ERS; AREI; Agricultural economics; Natural resources; Land; Land use; Land values; Land ownership; Water use; Irrigation; Water quality; Genetic resources; Biotechnology; Agricultural research; Agricultural productivity; Global resources; Soil conservation; Soil erosion; Pest management; Nutrient management; Animal agriculture; Organic agriculture; Conventional agriculture; Conservation policy; Land retirement; Working lands; Wetlands; Farmland protection; Environmental quality; Farm structure; Farm ownership; Farm management decisions; Farm business; Environmental Economics and Policy; Resource /Energy Economics and Policy.
Ano: 2006
URL: http://purl.umn.edu/7207

Agricultural Technology Choices for Poor Farmers in  Less-Favored Areas of South and East Asia
Provedor de dados: 31
Autores: Pender,   John L.
During the past several decades dramatic improvement has occurred in agricultural productivity and livelihoods in South and East Asia, stimulated by the Green Revolution and supported by several other factors. Nevertheless, hundreds of millions of rural people in less-favored environments of this region still live in poverty and received limited benefit from the Green Revolution. To address these problems, alternative technological approaches to the conventional Green Revolution technologies are being advocated to address the problems of poor farmers in less-favored areas of Asia, including low external input and sustainable agricultural approaches, organic agriculture and biotechnology. This paper reviews the literature on agricultural technology options...
Tipo: Working or Discussion Paper
Palavras-chave: Agricultural technologies; Low-external input and sustainable agriculture; Organic farming; Biotechnology; Less favored areas; Rural poverty; South and East Asia; Research and Development/Tech Change/Emerging Technologies.
Ano: 2007
URL: http://purl.umn.edu/42404

Allele-specific primer based identification of dimeric alpha-amylase inhibitor
Provedor de dados: 17
Autores: Pradeep Sharma; Pooja Sharma; Manoj Saini; S. S. Singh.
Wheat is one of the most important staple food crops cultivated over 200 mha in the range of environment throughout the world. Wheat production must continue to increase by 2% annually, more particularly in developing world including south-east Asia. Besides increasing the inherent productivity of wheat, it is important to minimize the losses caused to production by various abiotic and biotic factors. Alpha&amp;#x2013;amylase inhibitors are attractive candidates for the control of seed weevils as these insects are highly dependent on starch as the energy source. They play an important role in the carbohydrate metabolism of many heterotrophic and autotrophic organisms. In this study, we aimed to make sequence comparison and phylogenetic relationship among...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/5596/version/1

Allelic Frequency of ABO And Rh D Blood Group Among The Banjara Caste Population of Akola District, Maharashtra , India
Provedor de dados: 17
Autores: Aravind B. Chavhan.
The distribution of ABO blood groups and Rh (D) factor has been studied among the Banjara Caste population of Akola District (Maharashtra). The O, A, B, and AB blood group percentage were recorded as 27.64%, 22.91%, 37.45% and 12% respectively. The Rh negative incidence was recorded as 02.55%. The allele frequencies of O, A, B and AB groups in the combined data were found to be 0.5196, 0.2880, and 0.1924 respectively and Rh (D) positive allelic frequency was 0.8405.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5771/version/1

Allergological and toxicological risk assessment of genetically modified (GM) plants - Development of a model system
Provedor de dados: 17
Autores: Alain  Steinmann; Kristin  Entzian; Birgit  Gr&amp;#xfc;mmer; Udo  Meyer.
Technical advances in biotechnology lead to a rising number of genetically modified plants and derived food and feed. Therefore, the international interest in adequate and standardized analytical methods increases to assess health risks in a rapid and low cost manner.&amp;#xd;&amp;#xa;To address this issue a decision support system (DSS), which will be based on baseline data and thresholds of key parameters, is going to be established in the framework of a joint project (BioOK). Determination of the potential allergenicity or toxicity of novel proteins caused by the genetic modification compared to near isogenic variants (NIV) is a key issue in this context. For the development of DSS the model plant &amp;#x201c;potato&amp;#x201d; was used to compare...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4441/version/1

Alternativas para a redução de custos na produção de mudas in vitro.
Provedor de dados: 14
Autores: RIBEIRO, J. M.; PINTO, M. dos S. T.; TEIXEIRA, S. L.
Este trabalho apresenta alternativas que podem contribuir para a redução de custos no processo de produção de mudas in vitro. Substituição de componentes do meio nutritivo, substituição da autoclavagem pela esterilização química do meio nutritivo e utilização de biorreatores de imersão temporária são algumas das alternativas abordadas na publicação. Trata-se de uma boa fonte informação para aqueles que desejam ampliar seus conhecimentos acerca do tema, que deve ser amplamente discutido, pois é aderente a um dos nossos maiores desafios: a produção de alimentos em complexos cenários climáticos
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Produção de mudas.; Biotecnologia; Cultura de Tecido; Micropropagação.; Biotechnology.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/982095

Amino acid features: a missing compartment of prediction of protein function
Provedor de dados: 17
Autores: Esmaeil Ebrahimie; Mansour  Ebrahimi; Mahdi  Ebrahimi.
Enormous computational efforts have been carried out to predict structure and function of protein. However, nearly all of these efforts have been focused on prediction of function based on primary nucleic acid sequence or modeling 3D structure of protein from its nucleic acid sequence. In fact, it seems that amino acid attributes, which is an intermediate phase between DNA/RNA and advanced protein structure, has been missed.&amp;#xd;&amp;#xa;From 2010, we examined the possibility of precise prediction of structural protein function based on amino acid features by improving the following three aspects of amino acid research: (1) Increasing the number of computationally calculated amino acid features, (2) Testing different feature selection (attribute...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6693/version/1

An albumin-derived peptide scaffold capable of binding and catalysis
Provedor de dados: 17
Autores: Immacolata Luisi; Silvia Pavan; Giampaolo Fontanive; Alessandro Tossi; Fabio Benedetti; Adriano Savoini; Elisa Maurizio; Riccardo Sgarra; Daniele Sblattero; Federico Berti.
We have identified a 101-amino-acid polypeptide derived from the sequence surrounding the IIA binding site of human albumin. The polypeptide contains residues that make contact with ligands as warfarin in the parent protein, and eight cysteine residues to form disulfide bridges, which stabilize the polypeptide structure. Seventy-four amino acids are located in six [alpha]-helical regions, with the remaining amino acids forming six connecting coil/loop regions. Codon usage optimization was used to express a GST fusion protein in E. coli in yields as high as 4 mg/l. This fusion protein retains its structural integrity and aldolase activity, the ability to direct the stereochemical outcome of a diketone reduction, and its binding capacity to warfarin and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2012
URL: http://precedings.nature.com/documents/6894/version/1

An Analysis of McLean County, Illinois Farmers' Perceptions of Genetically Modified Crops
Provedor de dados: 31
Autores: Chimmiri,   Nagesh; Tudor,   Kerry W.; Spaulding,   Aslihan D.
McLean County, Illinois farmers were surveyed in order to explore and analyze their perceptions of genetically modified crops and their genetically modified cropping decisions. Questionnaires were mailed to 400 randomly selected farmers, and 156 were returned. The 134 respondents who reported that they planned to plant crops in 2003 were asked to provide information about gender, age, education, and number of tillable acres farmed. Respondents were also asked if they had previously planted genetically modified crops and if they planned to plant either genetically modified corn or genetically modified soybeans in 2003. Finally, respondents were presented with 40 statements that pertained to biotechnology and genetically modified crops, and they were asked...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Genetically modified; Corn; Soybeans; Research and Development/Tech Change/Emerging Technologies; Q16.
Ano: 2005
URL: http://purl.umn.edu/19204

An Analysis of Pricing in the U.S. Cotton Seed Market
Provedor de dados: 31
Autores: Shi,   Guanming; Stiegert,   Kyle W.; Chavas,   Jean-Paul.
The purpose of the research in this paper is to investigate the impact of differentiated vertical strategies by agricultural biotechnology firms in the U.S. cottonseed market. The model advances the measurement of industry concentration to consider substitution/complementarity relationships among differentiated products delivered under different vertical structures. We find evidence of sub-additive pricing in the stacking of bundled biotech traits. Prices paid by farmers for cottonseed sold under vertical integration are found to be higher than under licensing. The model is flexible and allows for evaluation of the effects of changing market structures. The parameters on traditional measures of concentration indicate that higher concentration leads to...
Tipo: Conference Paper or Presentation
Palavras-chave: Modal Vertical Strategy; Imperfect competition; Cotton seed; Biotechnology; Agricultural and Food Policy; Demand and Price Analysis; Industrial Organization; L13; L4; L65.
Ano: 2009
URL: http://purl.umn.edu/51617

An analysis of trade related international regulations of genetically modified food and their effects on developing countries
Provedor de dados: 31
Autores: Gruere,   Guillaume P.
This paper reviews current trade-related regulations of genetically modified (GM) food and discusses their effects on developing countries. There is a large heterogeneity in current import approval and marketing policies of GM food worldwide. At the international level, the harmonization efforts are led by the Codex Alimentarius Commission, the Cartagena Protocol on Biosafety and the World Trade Organization. While internationally harmonized guidelines for safety approval have been finalized, we show that there is no clear consensus on labeling regulations for GM food, and there is an increasing risk of conflicts among international agreements. We analyze the GM food regulations of two large rich importers, Japan and the European Union (EU) and discuss...
Tipo: Report
Palavras-chave: Genetically modified food; Developing countries; Biotechnology; Biosafety; Trade regulation; Labeling; International trade; Tariff; International Relations/Trade.
Ano: 2006
URL: http://purl.umn.edu/55422

An approach able to escape the most common problem during protein Crystallization i.e. formation of salt crystal
Provedor de dados: 17
Autores: K. Awaneesh Singh; Patrick Celie; G. R. K. Rao; M. V. Jagannatham.
Formation and differentiation of salt crystal is a most common as well as challenging problem during protein crystallography. Although there are some methods used for their differentiation i.e. physical manipulation, birefringence, dye absorption and run a gel but all of them are totally depends on own perception and expertise as well as also require a good crystal (final stage crystal). To escape this problem the screening was done with PACT, JCSG and other automated screens as everyone do. But during optimization the additives/salts were ignored at first and optimization screen was made just based on different buffers and precipitant. After selecting suitable buffer, the type and percentage of precipitant was optimized. We got some ugly crystals and with...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4725/version/1

An attempt to understand Barstar, Barnase and Olfactory receptor protein folding problems using mathematical biological approach
Provedor de dados: 17
Autores: Arunava Goswami; Sk Sarif Hassan; Pabitra  Pal Choudhury.
Protein folding problem as attracted structural biologists immensely (1). Till date, correlation between X-ray crystallographic and NMR data are considered to be the best methods for determining structure of intra-cellular proteins. Generating crystals and finding correct experimental conditions for NMR are largly a gamble and resultant data processing highly time consuming. Even if, a large number of laboratories around the world and India make crystals of protein but majority of them fail to generate crystallographic data less than or equal to 10A resolution with currently available instruments. This means that X-ray crystallographic data have been generated from protein crystals with non-uniform lattices. We believe that a large portion of (chain of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4891/version/1

An Empirical Analysis of the Effects of Plant Variety Protection Legislation on Innovation and Transferability
Provedor de dados: 31
Autores: Srinivasan,   Chittur S.; Shankar,   Bhavani; Holloway,   Garth J.
Under the TRIPs Agreement, all member-countries of the World Trade Organization are required to provide an "effective" system of plant variety protection within a specific time frame. In many developing countries this has led to a divisive debate about the fundamental desirability of extending intellectual property rights to agriculture. But empirical studies on the economic impacts of PVP, especially its ability to generate large private sector investments in plant breeding and facilitate the transfer of technology, have been very limited. This paper examines two aspects of the international experience of PVP legislation thus far (i) The relationship between legislation, R&amp;D expenditures and PVP grants, i.e., the innovation effect, and (ii) The role...
Tipo: Conference Paper or Presentation
Palavras-chave: Plant variety protection; Biotechnology; Technology transfer; Crop Production/Industries.
Ano: 2002
URL: http://purl.umn.edu/24788

AN ERA OF CONFUSION: THE LAND GRANT RESEARCH AGENDA AND BIOTECHNOLOGY
Provedor de dados: 31
Autores: Weatherspoon,   Dave D.; Oehmke,   James F.; Raper,   Kellie Curry.
The Land Grant College and University (LGCU) research system is in a state of confusion. Public research privatization, industry concentration, and balancing money and the Land Grant mission are issues LGCUs currently face. The ag-biotechnology revolution complicates these issues. This paper provides four mission related policy recommendations for the LGCU system. Keywords: Biotechnology, Land Grant Mission, Research
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Land Grant Mission; Research; Research and Development/Tech Change/Emerging Technologies.
Ano: 2000
URL: http://purl.umn.edu/11559

AN INITIAL LOOK AT THE TOKYO GRAIN EXCHANGE NON-GMO SOYBEAN CONTRACT
Provedor de dados: 31
Autores: Parcell,   Joseph L.
Genetically modified organisms (GMOs) such as soybeans and corn have garnered considerable consumer attention due to the concern over potential effects from using these commodities as inputs into food production. In the simplest form, segregation of bioengineered and non-bioengineered crops is an identity-preserved system. For these identity-preserved systems to exist, there must be a market discovery mechanism in place whereby supply and demand factors interact to establish a market price. In May 2000, the Tokyo Grain Exchange began a nongenetically modified organism (non-GMO) soybean contract. This article describes information garnered from the first public-offered identity-preserved marketplace.
Tipo: Journal Article
Palavras-chave: Biotechnology; Genetically modified organisms; Identity-preserved markets; Non-GMO; Soybean contract; Tokyo Grain Exchange; Marketing.
Ano: 2001
URL: http://purl.umn.edu/14684

An innovative method for the biological control of Alternanthera philoxeroides
Provedor de dados: 17
Autores: Yusong Cao; Huiming Wang.
Biological invasion has become a serious global environmental problems and been considered as an important component of global change^1-2^. Invasions by alien species can have an impact at several levels of ecological complexity from genes to ecosystems^3-4^. Invasive alien species had seriously threated the biodiversity and natural ecosystems in China^5^, and caused economic losses by more than US$7 billion every year^6^. Alternanthera philoxeroides, commonly known as Alligator weed, is an invasive amphibious plant^7^. The effect of physical methods such as mechanical or artificial salvage, removal of A. philoxeroides were very limited. Moreover, these measure should be carried out very carefully or would easy lead to its rebound^8^. The mainly biological...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5267/version/1

An integrated solution for rapid biosensing combining linker free binding, freeze drying and high sensitivity ellipsometric detection
Provedor de dados: 17
Autores: Yongbai Yin; David R. McKenzie; Keith Fisher; Chuan Guo; Neil Nosworthy; Marcela M. Bilek.
A novel integrated biosensor methodology is proposed and demonstrated. The methodology utilizes a nitrogen-containing plasma polymer to achieve linker-free binding of biorecognition molecules that allows the sensor surface to be freeze dried for long shelf life under ambient conditions. The sensor is configured for single wavelength ellipsometric detection providing a low cost, versatile, and rapid sensing and diagnosis platform suitable for a wide range of applications and end-users. The merits of the methodology were demonstrated using three antigen-antibody pairs.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6764/version/1

An intelligent liposome that may deliver drug molecules in a well controlled fashion
Provedor de dados: 17
Autores: Dumitru Popescu; Alin Gabriel Popescu.
The passage of molecules, especially large ones, through the cellular membrane is a very important problem for some biotechnological applications, such as drug delivery. The appearance of pores in the lipid bilayer following some controlled mechanisms may be an adequate and interesting way. Some pores, named stochastic pores, can appear due to structural and dynamic properties of lipid bilayer, but others may be favored by mechanical tension induced by different ways. Recently, a sequence of 30-40 pores was observed in the same vesicle, a pore at a time, which can appear in vesicles stretched by optical induced mechanical tension. There are two very interesting biotechnological applications that require the increase of membrane permeability: gene therapy...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1220/version/1

An Olfactory Receptor Pseudogene whose Function emerged in Humans
Provedor de dados: 17
Autores: Peter Lai; Gautam Bahl; Maryse Gremigni; Valery  Matarazzo; Olivier Clot-Faybesse; Catherine Ronin; Chiquito J. Crasto.
Human olfactory receptor, hOR17-210, is identified as a pseudogene in the human genome. Experimental data has shown however, that the gene product of cloned hOR17-210 cDNA was able to bind an odorant-binding protein and is narrowly tuned for excitation by cyclic ketones. Supported by experimental results, we used the bioinformatics methods of sequence analysis, computational protein modeling and docking, to show that functionality in this receptor is retained due to sequence-structure features not previously observed in mammalian ORs. This receptor does not possess the first two transmembrane helical domains (of seven typically seen in GPCRs). It however, possesses an additional TM that has not been observed in other human olfactory receptors. By...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1290/version/1

Anais...
Provedor de dados: 14
Autores: SEMANA DA CAPRINOCULTURA E DA OVINOCULTURA TROPICAL BRASILEIRA, 1., 1994, Sobral.
Aspectos economicos da caprino-ovinocultura tropical brasileira; Caprinocultura leiteira no Brasil - estadio da arte e perspectivas; Estadio atual e perspectivas da ovinocultura tropical; Biotecnologia da reproducao caprina; Tecnologia e comercializacao de carne ovina; Industrializacao e comercializacao de pele; A modernidade cientifica e sua interfaces.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Aspecto econômico; Congresso; Brasil; Ceara; Congress.; Caprinocultura; Ovinocultura; Comercialização; Mercado; Biotecnologia; Goats; Sheep; Commercialization; Biotechnology; Small ruminants; Brazil.
Ano: 1994
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/514313

Análise da expressão gênica pela técnica de PCR quantitativa em tempo real: princípios e fundamentos.
Provedor de dados: 14
Autores: MORGANTE, C. V.; BLAWID, R.
Neste trabalho são apresentados os conceitos fundamentos da análise da expressão gênica pela técnica de PCR e reúne informações importantes para aqueles que se dedicam ao estudo da genética vegetal. Sumário: Introdução; Rermos básicos utilizados na qPCR; Sistemas para a detecção da fluorescência; Etapas anteriores à reação de qPCR; Parâmetros para a padronização da qPCR; Métodos de quantificação gênica; Referências; Glossário.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: PCR; Metodologia de análise; Biologia molecular; Genética vegetal; Biotecnologia; Biotechnology.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1066218

Análise da variabilidade genética molecular de uma coleção de trabalho de guandu (Cajanus cajan) como apoio na seleção de acessos para testes agronômicos em sistemas de integração lavoura-pecuária e recuperação de pastagens degradadas.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FERNANDES, F. D.; BARCELLOS, A. O.; ANDRADE, R. P.; BELLON, G.; JUNQUEIRA, K. P.; AMABILE, R. F.; MARTHA JÚNIOR, G. B.; VILELA, L.; RAMOS, A. K. B.; KARIA, C. T.; GODOY, R.
bitstream/CPAC-2009/27696/1/p2006_35.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Feed legumes.; Biotecnologia; Marcador Molecular; Leguminosa Forrageira; Marcador Genético; Variação Genética.; Genetic variation.; Biotechnology; Genetic markers.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570173

Analysis for biotechnology innovations using Strategic Environmental Assessment (SEA)
Provedor de dados: 31
Autores: Lanacre,   Nicholas A.; Gaskell,   Joanne; Rosegrant,   Mark W.; Falck-Zepeda,   Jose Benjamin; Quemada,   Hector; Halsey,   Mark; Birner,   Regina.
Meeting the food needs of the world’s growing population while reducing poverty and protecting the environment is a major global challenge. Genetically modified crops appear to provide a promising option to deal with this challenge. However there is a need to make strategic decisions on how to spend limited agricultural research funds in order to achieve a maximum impact with regard to finding sustainable solutions to end hunger and poverty. In international development institutions, there is growing interest in the potential use of Strategic Environmental Assessment (SEA) as part of a research based Environmental Management System (EMS) to promote mainstreaming of environmental considerations in policy development. SEA was developed as an approach to...
Tipo: Working or Discussion Paper
Palavras-chave: Risk; Strategic Environmental Assessment; Genetically Modified; GMO; LMO; Living Modified Organisms; Biotechnology; Environmental risk; Environmental Economics and Policy; Research and Development/Tech Change/Emerging Technologies.
Ano: 2005
URL: http://purl.umn.edu/58584

Analysis of acceptance of bacillus to various heavy metals and antibiotics
Provedor de dados: 17
Autores: Yamini Singh  Sisodia; Vivek  Bajpai; Deeksha  Gaur; Pankaj Kumar  Jain.
The present study was carried out to systematically examine the pathogenic microorganisms in sewage samples isolated from the Shekhawati region of Rajasthan. In this survey, 26 bacterial strains were isolated and identified using biochemical and molecular methods. Molecular identification of isolates was done by DNA isolation, PCR amplification using 16S rDNA primer and partial sequencing of purified product after sequencing, out of 26, 10 sequences were submitted to NCBI. Out of these 10 isolates Bacillus pumilis, Bacillus subtilis and Bacillus licheniformis were taken for further studies. There is growing awareness of the need for development of new antimicrobial agents for the treatment of human, animal and plant diseases. Keeping this fact in mind, we...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7073/version/1

Analysis of plant metabolites &amp;#x2013; the baseline-threshold principle
Provedor de dados: 17
Autores: Katja  Neubauer; Jenny Bandomir; Anett  Grunenberg; Andreas  M&amp;#xfc;ller; Udo  Kragl; Kerstin  Schmidt.
With the environmental risk assessment (ERA) for genetically modified plants (GMP) the identification of equivalence or differences in the composition of ingredients of GMPs and non-GMPs has to be investigated. The strategy is based upon the idea that traditionally cultivated crops have been used safely for many years, thus every divergence within the compounds of a GMP in contrast to its non-GMP counterpart could refer to a risk and has to be further analysed.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The BioOK company offers the opportunity to analyse very different aspects from the ERA of GMPs in one hand. Beside toxicological analysis and the determination of environmental effects via soil analysis, we can look into several plant metabolites to identify...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4439/version/2

Analysis of plant metabolites &amp;#x2013; the baseline-threshold principle
Provedor de dados: 17
Autores: Katja  Neubauer; Jenny Bandomir; Anett  Grunenberg; Andreas  M&amp;#xfc;ller; Udo  Kragl; Kerstin  Schmidt.
With the environmental risk assessment (ERA) for genetically modified plants (GMP) the identification of equivalence or differences in the composition of ingredients of GMPs and non-GMPs has to be investigated. The strategy is based upon the idea that traditionally cultivated crops have been used safely for many years, thus every divergence within the compounds of a GMP in contrast to its non-GMP counterpart could refer to a risk and has to be further analysed.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The BioOK company offers the opportunity to analyse very different aspects from the ERA of GMPs in one hand. Beside toxicological analysis and the determination of environmental effects via soil analysis, we can look into several plant metabolites to identify...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4439/version/1

Analysis of Scientific Research Driving Microalgae Market Opportunities in Europe
Provedor de dados: 5
Autores: Rumin, Judith; Nicolau, Elodie; Junior, Raimundo Gonçalves De Oliveira; Fuentes-grünewald, Claudio; Picot, Laurent.
A bibliographic database of scientific papers published by authors affiliated to research institutions worldwide, especially focused in Europe and in the European Atlantic Area, and containing the keywords “microalga(e)” or “phytoplankton” was built. A corpus of 79,020 publications was obtained and analyzed using the Orbit Intellixir software to characterize the research trends related to microalgae markets, markets opportunities and technologies that could have important impacts on markets evolution. Six major markets opportunities, the production of biofuels, bioplastics, biofertilizers, nutraceuticals, pharmaceuticals and cosmetics, and two fast-evolving technological domains driving markets evolution, microalgae harvesting and extraction technologies...
Tipo: Text
Palavras-chave: Bibliometric; Microalgae; Biofertilizers; Bioplastics; Biotechnology; Cosmetics; Europe; Food; GMO; Market; Nutraceuticals; Pharmaceuticals; Research.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00630/74185/73788.pdf

Animal Disease and the Industrialization of Agriculture
Provedor de dados: 31
Autores: Hennessy,   David A.; Wang,   Tong.
The industrialization of animal agriculture has fundamentally transformed animal health markets while animal health innovations have promoted this industrialization. The subtlety of these interactions shows how little we know about agricultural industrialization. To illustrate, we consider three stylized features of industrialized animal agriculture. These are the closing off of production activities from external effects, emphasis on control, and use of biosecurity measures. We find that animal disease externalities should lead to higher stocking on any given farm, and also to deficient entry into animal production. Eradicating the disease in a region increases both the stocking rate per farm and the number of farms. We show that antibiotics as a control...
Tipo: Working or Discussion Paper
Palavras-chave: Animal disease; Biosecurity; Biotechnology; Competitiveness; Confined animal agriculture; Economies of scale; Tragedy of the commons; Veterinary inputs.; Agricultural and Food Policy; Livestock Production/Industries; Production Economics; Research and Development/Tech Change/Emerging Technologies.
Ano: 2010
URL: http://purl.umn.edu/93673

Antártica: fuente de recursos biológicos para la biotecnología nacional
Provedor de dados: 20
Autores: Blamey, J.
En diciembre del 2007, Innova-Chile de CORFO aprobó el financiamiento del proyecto “Antártica: Fuente de recursos biológicos para la biotecnología nacional”. Este proyecto tiene como fin crear una plataforma que facilite el acceso a los recursos antárticos de microorganismos y plantas, de una forma que permitirá su valorización para la biotecnología chilena y su desarrollo científico en el área. Por sus únicas condiciones de aislamiento y bajas temperaturas, la Antártica alberga un potencial científico y biotecnológico enorme, pero también muy altas barreras de entrada de diferente índole.
Tipo: Journal Contribution
Palavras-chave: Biotechnology; Microorganisms; Flora; Biotechnology; Microorganisms; Flora; Http://aims.fao.org/aos/agrovoc/c_16165; Http://aims.fao.org/aos/agrovoc/c_4807; Http://aims.fao.org/aos/agrovoc/c_2983.
Ano: 2008
URL: http://hdl.handle.net/1834/3237

Antibody-antigen interactions: What is the required time to equilibrium?
Provedor de dados: 17
Autores: Karl Andersson; Hanna Bj&amp;#xf6;rkelund; Magnus Malmqvist.
The use of antibodies is widespread in many areas including in-vivo and in-vitro diagnostics, quantitative analysis in research laboratories and as therapeutic substances. Since the methods for generation of antibodies has improved and regularly results in high-affinity interactions, the standard assays used for quantification of the interaction properties should be revisited because they do not necessarily produce accurate results. Here we show that in several cases, the affinity determination of strongly binding antibodies will be inherently difficult when using standard procedures, due to impractically long incubation times. Real-time kinetic analysis is often the only realistic alternative for affinity determination.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Microbiology.
Ano: 2010
URL: http://precedings.nature.com/documents/5218/version/1

Antiviral activity of (E)-cinnamaldehyde revisited with nanoscience tools
Provedor de dados: 17
Autores: Arunava Goswami; Ayesha Rahman.
Contradictory results have been reported regarding the anti-viral activity of (E)-cinnamaldehyde, a major constituent (~69%) of cinnamon. Here we show that (E)-cinnamaldehyde alone has very low antiviral property contrary to the belief of commoners. There are early sporadic reports in ancient medicinal practices that fine sand was used for increasing the efficacy of antiviral drugs. Can we increase the efficacy of (E)-cinnamaldehyde marginally by using one of the major constituents of sand like silica? Yes, when nanosilica is used as a carrier during (E)-cinnamaldehyde administration, the antiviral efficacy of the resultant cocktail increases marginally. Therefore, (E)-cinnamaldehyde consumed for centuries in tribal therapy as well as in alternative...
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/5043/version/1

Aplicação de descritores morfoagronômicos utilizados em ensaios de DHE de cultivares de maracujazeiro-azedo (Passiflora edulis Sims): manual prático.
Provedor de dados: 14
Autores: JESUS, O. N. de; OLIVEIRA, E. J. de; SOARES, T. L.; FALEIRO, F. G.
bitstream/item/145206/1/jesus-01.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Maracujá-azedo; Cultivar; Melhoramento genético; Passion fruit.; Biotecnologia; Passiflora Edulis.; Genetic improvement; Biotechnology; Varieties.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1048462

Aplicação de descritores morfoagronômicos utilizados em ensaios de DHE de cultivares de maracujazeiro-doce, ornamental, medicinal, incluindo espécies silvestres e híbridos interespecíficos (Passiflora spp.): manual prático.
Provedor de dados: 14
Autores: JESUS, O. N. de; OLIVEIRA, E. J. de; SOARES, T. L.; FALEIRO, F. G.
bitstream/item/146347/1/jesus-02.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Maracujá-doce; Passiflora spp; Cultivar; Melhoramento genético; Passion fruit.; Biotecnologia.; Genetic improvement; Biotechnology; Varieties.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1048464

Applications Of Microspectroscopy, Hyperspectral Chemical Imaging And Fluorescence Microscopy In Chemistry, Biochemistry, Biotechnology, Molecular And Cell Biology
Provedor de dados: 17
Autores: I. C. Baianu.
Chemical imaging is a technique for the simultaneous measurement of spectra (chemical information) and images or pictures (spatial information)^1,2^. The technique is most often applied to either solid or gel samples, and has applications in chemistry, biology^3-8^, medicine^9,10^, pharmacy^11^ (see also for example: Chemical Imaging Without Dyeing), food science, Food Physical Chemistry, Biotechnology^12,13^, Agriculture and industry. NIR, IR and Raman chemical imaging is also referred to as hyperspectral, spectroscopic, spectral or multi-spectral imaging (also see micro-spectroscopy). However, other ultra-sensitive and selective, chemical imaging techniques are also in use that involve either UV-visible or fluorescence microspectroscopy.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6593/version/1

Apply of Textmining Method to Study the Roles in Improving the Health by Lactoferrin, a Multi-Functional Milk Protein
Provedor de dados: 17
Autores: Kei-ichi K. Shimazaki; Tatsuya T. Kushida; Koji K. Yamauchi; Toshihisa T. Takagi.
Lactoferrin is a metal-binding glycoprotein found in milk, blood and other exocrine secretions.  This is a multi-functional protein that exhibits many activities such as: anti-microbial, anti-viral, immunomodulatory, anti-inflammatory, anti-tumor, anti-metastatic, cell growth-promoting, and anti-oxidant activities, as well as regulation of granulopoiesis and iron absorption, etc.  To date, a number of academic reports concerning the biological activities of lactoferrin have been published and are easily accessible through public databases.  In order to overcome the information overload associated with lactoferrin information, we have applied the text mining method to the accumulated lactoferrin literature.  To this end, we used the information extraction...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/5100/version/1

Applying of novel subtracted method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes
Provedor de dados: 17
Autores: Ewa Siedlecka; Aneta Hromada-Judycka; Magdalena Pawe&amp;#x142;kowicz; Rafa&amp;#x142; W&amp;#xf3;ycicki; Monika Rakoczy-Trojanowska; Zbigniew Przybecki.
We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate tags carrying the polymorphisms. &amp;#xd;&amp;#xa;To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for requested traits.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics &amp; Genomics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5465/version/1

Applying of novel subtraction method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes
Provedor de dados: 17
Autores: Ewa Siedlecka; Aneta Hromada-Judycka; Magdalena Pawe&amp;#x142;kowicz; Rafa&amp;#x142; W&amp;#xf3;ycicki; Monika Rakoczy-Trojanowska; Zbigniew Przybecki.
We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate tags carrying the polymorphisms. &amp;#xd;&amp;#xa;To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for requested traits.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics &amp; Genomics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5465/version/2

Aptamer-based multiplexed proteomic technology for biomarker discovery
Provedor de dados: 17
Autores: Larry Gold; Deborah Ayers; Jennifer Bertino; Christopher Bock; Ashley Bock; Edward N. Brody; Jeff Carter; Virginia Cunningham; Andrew Dalby; Bruce E. Eaton; Tim Fitzwater; Dylan Flather; Ashley Forbes; Trudi Foreman; Cate Fowler; Bharat Gawande; Meredith Goss; Magda Gunn; Shashi Gupta; Dennis Halladay; Jim Heil; Joe Heilig; Brian Hicke; Gregory Husar; Nebojsa Janjic; Thale Jarvis; Susan Jennings; Evaldas Katilius; Tracy R. Keeney; Nancy Kim; Terese Kaske; Tad Koch; Stephan Kraemer; Luke Kroiss; Ngan Le; Daniel Levine; Wes Lindsey; Bridget Lollo; Wes Mayfield; Mike Mehan; Robert Mehler; Michele Nelson; Sally K. Nelson; Dan Nieuwlandt; Malti Nikrad; Urs Ochsner; Rachel M. Ostroff; Matt Otis; Thomas Parker; Steve  Pietrasiewicz; Dan Resnicow; John Rohloff; Glenn Sanders; Sarah Sattin; Dan Schneider; Britta Singer; Martin Stanton; Alana Sterkel; Alex Stewart; Suzanne Stratford; Jonathan D. Vaught; Mike Vrkljan; Jeffrey J. Walker; Mike Watrobka; Sheela Waugh; Allison Weiss; Sheri Wilcox; Alexey Wolfson; Steve Wolk; Chi Zhang; Dom Zichi.
Interrogation of the human proteome in a highly multiplexed and efficient manner remains a coveted and challenging goal in biology.  We present a new aptamer-based proteomic technology for biomarker discovery capable of simultaneously measuring thousands of proteins from small sample volumes (15 [mu]L of serum or plasma).  Our current assay allows us to measure ~800 proteins with very low limits of detection (1 pM average), 7 logs of overall dynamic range, and 5% average coefficient of variation.  This technology is enabled by a new generation of aptamers that contain chemically modified nucleotides, which greatly expand the physicochemical diversity of the large randomized nucleic acid libraries from which the aptamers are selected.  Proteins in complex...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4538/version/1

Aqueous synthesis of CdTe quantum dot as biological fluorescent probe for monitoring methyl parathion by fluoro-immunosensor
Provedor de dados: 17
Autores: Raghuraj Chouhan; Aaydha Vinayaka; Munna Thakur.
Bioconjugation of quantum dots (QDs) provide high resolution in biological fluorescent labelling as a result of physical and optical properties of QDs. This intrinsic property of QDs can be made use of for sensitive detection of target analytes including food and environmental monitoring. In this investigation, we report the bioconjugation of thiol stabilized CdTe QD for the sensitive detection of methyl parathion (MP) at picogram level. The specificity in the analysis was attributed by highly specific competitive immunological reactions between free MP and CdTe QD bioconjugated MP (MP-BSA-CdTe) for immobilized anti-MP IgY antibodies in a simple flow injection system. We also report the possible resonance energy transfer phenomenon as a result of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3451/version/1

Arbuscular mycorrhizal fungus spores host bacteria and their biofilm efficient in nutrient biodynamics and soil-borne plant pathogen suppression
Provedor de dados: 17
Autores: Andre Cruz; Takaaki Ishii.
The combination of bacteria and arbuscular mycorrhizal fungi (AMF) would be ideal for nutrient biodvnamics in soils and protection from soil-borne plant pathogens (SBPP). During the process of plant colonization AMF interact with bacteria where spores cytoplasm and hvphae provide specific niches for certain populations of bacteria. The clarification of the relationship AMF-bacteria has great potential for sustainable agriculture. The currently research aimed to isolate and characterize bacteria. Which we have designated most probable endobacteria (MPE) from an AMF spore. Three bacterial strains were taken from about 500 spores by using a 200 &amp;#xb5;m diameter hvpodermic needle. These bacteria were identified by DNA and morphological methods. Antagonism...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2011
URL: http://precedings.nature.com/documents/5544/version/1

Archon Genomics X PRIZE Validation Protocol
Provedor de dados: 17
Autores: Larry Kedes; Granger Sutton; Edison  Liu; Victor Jongeneel.
This document is a collective assembly of techniques designed to test the quality and accuracy of 100 whole human genome sequences resulting from the $10 Million Archon Genomics X PRIZE (AGXP) competition. The purpose of this article is to enlist constructive criticism from the genomic and genetic community on the outlined approaches. The intent for the final version of this Validation Protocol is to become a useful standard by which to gauge the capabilities of whole genome sequencing technologies that emerge even after 2012.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5731/version/1

Artificial Antigen Presenting Cells With Preclustered anti-CD28/-CD3/-LFA-1 Monoclonal Antibodies Are Highly Effective To Induce The Ex-Vivo Expansion Of Functional Human Antitumor T Cells
Provedor de dados: 17
Autores: Roberta Zappasodi; Massimo Di Nicola; Carmelo Carlo-Stella; Roberta Mortarini; Alessandra Molla; Claudia Vegetti; Lorena Passoni; Salvatore Albani; Andrea Anichini; Alessandro M. Gianni.
Effective adoptive T cell therapy requires the _ex vivo_ generation of functional T lymphocytes with a long lifespan _in vivo_. We evaluated _in vitro_ T cell expansion by artificial antigen presenting cells (aAPC) generated with activating (human anti-CD3), co-stimulating (human anti-CD28) and adhesion (human anti-LFA-1) monoclonal antibodies pre-clustered in microdomains (MDs) held by a liposome scaffold. The co-localization of T cell ligands in MDs and the targeting of an adhesion protein, increasing the efficiency of immunological synapse formations, represent the novelties of our system. These aAPCs allowed increased expansion of polyclonal CD4^+^ and CD8^+^ T cells and of tumor antigen-specific CD8^+^ T cells compared to anti-CD28- and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Immunology.
Ano: 2007
URL: http://precedings.nature.com/documents/1250/version/1

Artificial Photosynthesis Would Unify the Electricity-Carbohydrate-Hydrogen Cycle for Sustainability
Provedor de dados: 17
Autores: Yi-Heng Percival Zhang.
Sustainable development requires balanced integration of four basic human needs &amp;#x2013; air (O2/CO2), water, food, and energy.  To solve key challenges,  such as CO2 fixation, electricity storage, food production, transportation fuel production, water conservation or maintaining an ecosystem for space travel,  we wish to suggest the electricity-carbohydrate-hydrogen (ECHo) cycle, where electricity is a universal energy carrier, hydrogen is a clean electricity carrier, and carbohydrate is a high-energy density hydrogen (14.8 H2 mass% or 11-14 MJ electricity output/kg)carrier plus a food and feed source.  Each element of this cycle can be converted to the other reversibly &amp;#x26; efficiently depending on resource availability, needs, and costs.  In...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Ecology; Earth &amp; Environment.
Ano: 2010
URL: http://precedings.nature.com/documents/4167/version/1

Asian soybean rust in Brazil: past, present, and future
Provedor de dados: 86
Autores: Godoy,Cláudia Vieira; Seixas,Claudine Dinali Santos; Soares,Rafael Moreira; Marcelino-Guimarães,Franscismar Correa; Meyer,Maurício Conrado; Costamilan,Leila Maria.
Abstract: Asian soybean rust, caused by the fungus Phakopsora pachyrhizi, is the most severe disease of the crop and can cause yield losses of up to 90%. The disease was first reported in Brazil in 2001. Epidemics of the disease are common in the country, where the fungus can survive year-round. Regulatory measures to reduce the inoculum between seasons and avoid late-season soybean have been adopted to manage the disease. Disease control has relied mainly on chemical control, but a lower sensibility of the fungus to fungicides has been reported in Brazil. Major-resistance genes have been mapped and incorporated into the cultivars. With the reduced efficacy of the fungicides, the adoption of integrated measures to control the disease will be important for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Phakopsora pachyrhizi; Biotechnology; Chemical control; Genetic resistance; Losses; Management.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500407

Assessment of Biotechnology Policies and International Trade in Key Markets for U.S. Agriculture
Provedor de dados: 31
Autores: Marchant,   Mary A.; Song,   Baohui.
The United States leads the world in agricultural biotechnology research, adoption, commercialization, and exports.  Our biotech commodities are highly dependent on international markets.  Thus, any biotech policy changes by key importing countries may affect U.S. agricultural biotech product exports.  This article identifies key markets for U.S. agricultural exports including biotech commodities and discusses current and proposed biotech policies in key markets for U.S. agricultural exports focusing on Canada, Mexico, Japan, the European Union (EU), and China.  Among these markets, labeling of biotech products is voluntary in Canada and Mexico but is mandatory in Japan, the EU, and, most recently, in China.  For the EU, U.S. corn exports were almost...
Tipo: Journal Article
Palavras-chave: Biotechnology; Biotech policy; Corn; Cotton; International trade; Soybeans; U.S. agricultural exports; Q13; Q17; Q16; Q18.
Ano: 2005
URL: http://purl.umn.edu/43513

Automacao e racionalizacao na micropropagacao industrial.
Provedor de dados: 14
Autores: FARI, M.; MELO, N. F. de.
Tendencias da mecanizacao na propagacao in vitro em larga escala, automacao das etapas dos metodos tradicionais de cultura de tecidos, novastecnologias baseadas na utilizacao de sistemas de propagacao em meio liquido, desenvolvimento de sistemas de cultivo in vitro alternativos, sistema CLONER: uma nova iniciativa de racionalizacao da bancada de micropropagacao.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Propagacao in vitro; In vitro propagation; Biotecnologia; Cultura de Tecido; Meristema; Micropropagação; Biotechnology; Micropropagation; Tissue culture.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131977

Avaliacao do fluxo genico entre a cultivar de soja BR-16 transgenica e BR-16 nao-transgenica no ecossistema do Cerrado no Distrito Federal.
Provedor de dados: 14
Autores: ABUD, S.; SOUZA, P. I. M.; MOREIRA, C. T.; VIANNA, G. R.; ANDRADE, S. R. M.; RECH, E. L.; ARAGAO, F. J. L.
bitstream/CPAC-2009/24176/1/p2002_67.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Biotecnologia; Glycine Max; Melhoramento Genético Vegetal; Planta Transgênica; Soja.; Biotechnology; Plant breeding; Transgenic plants.; Soybeans.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567487

Bacillus EFFECT ON THE GERMINATION AND GROWTH OF TOMATO SEEDLINGS (Solanum lycopersicum L)
Provedor de dados: 37
Autores: CABRA CENDALES,Teresa; RODRÍGUEZ GONZÁLEZ,Cristian Alonso; VILLOTA CUÁSQUER,Claudia Patricia; TAPASCO ALZATE,Omar Alberto; HERNÁNDEZ RODRÍGUEZ,Annia.
ABSTRACT The capacity to solubilize phosphate and to produce indole compounds Indole Acetic Acid type, was evaluated in 15 strains isolated from castor bean lignocellulosic residues (Ricinus communis). To determine the solubilizing activity of phosphates a qualitative test by using Pikovskaya culture medium was employed and for the evaluation of the production of indole compounds (IAA) a Salkowsky colorimetric analysis technique was applied. Among the microorganisms tested, the Bacillus pumilus GIBI 206 demonstrated capacity to solubilize phosphates and Bacillus subtilis GIBI 200 showed of capacity to solubilize phosphates and to produce Indoleacetic Acid (IAA). To determine the effect of the Bacillus subtilis strain on germination and growth promotion,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Indole; PGPB; Seedlings; Tomato.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000100004

Bacterias antárticas y agentes antibacterianos
Provedor de dados: 20
Autores: Domínguez, M.
La Antártica se ha constituido en una región de gran interés científico debido a las condiciones extremas que allí se presentan, en especial, de temperatura y radiación ultravioleta. En el campo de la microbiología, las investigaciones se han enfocado, principalmente, en el estudio de la  biodiversidad bacteriana, lo que ha llevado a la descripción de nuevas especies, y también gran interés ha despertado la producción de compuestos con aplicaciones  biotecnológicas. Entre éstos se encuentran los que pudieran presentar actividad antibacteriana, de gran importancia debido a que uno de los mayores problemas de los últimos años es la emergencia de patógenos intrahospitalarios con una amplia resistencia a los agentes antibacterianos disponibles, no existiendo...
Tipo: Journal Contribution
Palavras-chave: Bacteria; Biotechnology; Biotechnology; Bacteria; Http://aims.fao.org/aos/agrovoc/c_16165; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 2008
URL: http://hdl.handle.net/1834/3372

Bark Extract of Bathysa cuspidata in the Treatment of Liver Injury Induced by Carbon Tetrachloride in Rats
Provedor de dados: 52
Autores: Gonçalves,Reggiani Vilela; Matta,Sérgio Luis Pinto da; Novaes,Rômulo Dias; Leite,João Paulo Viana; Peluzio,Maria do Carmo Gouveia; Vilela,Emerson Ferreira.
The aim of this study was to investigate the effect of bark extract Bathysa cuspidata (BCE) in the reconstitution of hepatic parenchyma and stroma after dysfunction induced by the CCl4. Liver lesions were induced by intraperitoneal administration of CCl4 (1 mL/kg) every 48 h for 12 days. The animals were treated with B. cuspidata extract (BCE) administered by gavage for another 12 days. Forty-nine rats were randomized into seven treatment groups with seven animals receiving CCl4, BCE (200, or 400 mg/kg) and the vehicle DMSO alone, or in different combinations. The extract alone showed no evidence of hepatic toxicity. In general, rats acutely exposed to CCl4 without the treatment with BCE presented high ALT and AST serum levels and high tissue content of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant defense; Biotechnology; Hepatotoxicity; Phytotherapy; Pathology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400504

BBSRC Data Sharing Policy
Provedor de dados: 17
Autores: Amanda Collis; David McAllister; Michael Ball.
BBSRC recognizes the importance of contributing to the growing international efforts in data sharing. BBSRC is committed to getting the best value for the funds we invest and believes that making research data more readily available will reinforce open scientific inquiry and stimulate new investigations and analyses. BBSRC supports the view that data sharing should be led by the scientific community and driven by scientific need. It should also be cost effective and the data shared should be of the highest quality. Members of the community are expected and encouraged to practice and promote data sharing, determine standards and best practice, and create a scientific culture in which data sharing is embedded.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics &amp; Genomics; Immunology; Microbiology; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics; Plant Biology; Evolutionary Biology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6015/version/1

Beyond the fuzzy lock-and-key: spontaneous symmetry shifts and glycan/lectin logic gates
Provedor de dados: 17
Autores: Rodrick Wallace.
Changes in the molecular topology of glycan/lectin interaction may explain observed reaction punctuation driven by experimental gradients in reactant concentration. Adoption of a &amp;#x27;biological renormalization&amp;#x27; perspective from statistical physics for the analysis of such phase transitions suggests, in marked contrast to conventional physical systems, a broad spectrum of possible universality class behaviors. This spectrum may, in typical perverse biological manner, be of central scientific interest. Generalization, via formalism abducted from coevolutionary theory, suggests that glycan/lectin molecular switches instantiate logic gates that may be as sophisticated as those characterizing basic neural process, if on a different scale.
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology; Molecular Cell Biology; Pharmacology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6849/version/1

BioBrick Promoter Measurement Kit Instruction Sheets
Provedor de dados: 17
Autores: Jason Kelly.
Instructions for inserting a test promoter into the measurement kit plasmid for characterization as well as instructions for measuring the activity of the test promoter in Standard Promoter Units (SPUs).  These instructions were distributed to the teams participating in the International Genetically Engineered Machines competition (iGEM).
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/2028/version/1

Bioconversion of eugenol into food flavouring agent vanillin
Provedor de dados: 17
Autores: Suaib  Luqman; Sudeep  Tandon; Alok  Somvanshi; Suchita  Srivastava; Mahendra  P. Darokar; Suman  P. S. Khanuja.
Microorganisms have the ability to chemically modify a wide variety of organic compounds by a process referred to as biological or microbial transformation, or in general, bioconversion. The microbial cells and their catalytic machinery (enzymes) accept a wide array of complex molecules as substrates, yielding products with unparallel chiral (enantio-), positional (region-) and chemical (chemo-) selectivity through various biochemical reactions. The present study was formulated on the objective of the conversion of abundantly available phytomolecules eugenol into vanillin, a compound of industrial importance, using microorganisms Aspergillus flavus, Aspergillus niger and Pseudomonas aeruginosa. These microbes were found to be capable of converting eugenol...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6926/version/1

Biodegradable Luminescent Silicon Quantum Dots for Two Photon Imaging Applications
Provedor de dados: 17
Autores: Chen-An  Tien; Folarin Erogbogbo; Ching-Wen  Chang; Priscilla  Adjei-Baffour; Wing-Chueng Law; Mark T. Swihart.
Cadmium- and lead-based quantum dots are normally coated for biological applications, because their degradation may result in the release of toxic heavy metal ions. Here, we synthesize silicon quantum dots that are expected to biodegrade to non-toxic products. A chitosan coating is used to render the silicon quantum dots stable in storage conditions and biodegradable at physiological conditions. The applications of these particles are demonstrated in cellular imaging with single and two-photon excitation. These results open the door for a new generation of silicon quantum dots that may have a wide variety of applications derived from the flexibility of chitosan.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2012
URL: http://precedings.nature.com/documents/7012/version/1

Bioenergetics Potential of RR2 PRO Transgenic Soybean Subjected to Application of Herbicides Isolated and in Combination
Provedor de dados: 52
Autores: Moreno,Giovane; Santos,Viviane Taisa; Albrecht,Leandro Paiola; Cesco,Victor Salomão.
ABSTRACT In Brazil, there are several crops producing oil for biofuel production, such as canola, sunflower, peanut, cotton, castor bean, soybean, among others. There are parameters that indicate the energetic and economic viability for the production of biofuels, and that can be applied, for example, in transgenic soybeans. The present study aimed to evaluate the energetic viability of Intacta RR2 PROTM soybean for biofuel production.Experiments were conducted in the municipalities of Palotina and Marechal Cândido Rondon, both in the State of Paraná. Energy balance calculations were carried out from the production system, estimating energy expenditure, including the agricultural and industrial stages. The inputs used were considered as input of energy,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Bioenergy; Biotechnology; Energy generation; Socioeconomic evaluation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200229

BioenergyProduction From Plant Biomass: Bioethanol From Concept To Reality
Provedor de dados: 17
Autores: Israr  Khan; Muhammad Waheed Akhtar.
Natural energy reserves like petrol and diesel etc are depleting.  As such, it is of paramount importance to find alternative fuels. Solar energy is trapped as biomass in plants. Biomass provide an excellent reserve for trapping solar energy on field. Complex enzymatic machinery comprising of hydrolytic reactions  and consolidated bioprocessing is needed to make viable bioenergy, mostly biofuels like bioethanol for locomotives, industries and general use in the future.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6286/version/1

Biological Limits on Agricultural Intensification: An Example from Resistance Management
Provedor de dados: 31
Autores: Laxminarayan,   Ramanan; Simpson,   R. David.
When the application of pesticides places selective evolutionary pressure on pest populations, it can be useful to plant refuge areas-crop areas intended to encourage the breeding of pests that are susceptible to the pesticide. Renewed interest in refuge areas has arisen with recent advances in biotechnology and genetically modified (GM) crops. In this paper, we use a simple model of the evolution of pest resistance to characterize the socially optimal refuge strategy for managing pest resistance. We demonstrate some interesting analogies with other models of renewable resource management, such as those of fisheries. Among the analogous results are findings that maintaining what we might call "maximal sustainable susceptibility" is typically not...
Tipo: Working or Discussion Paper
Palavras-chave: Pest resistance; Biotechnology; Optimization; Research and Development/Tech Change/Emerging Technologies; Q1; Q2.
Ano: 2000
URL: http://purl.umn.edu/10914

Biological robotics and nanorobotred cells: Characterization and applications
Provedor de dados: 17
Autores: Rakesh Sharma; Soonjo Kwon; Ching J. Chen.
In human body, nanorobot-like red blood cells get exposed to human body fluids and many physico-chemical changes inside while these nanorobots (fullerene structure coated hemoglobin embedded iron inorganic elements) remain safe and perform killer action against bacteria or virus. We propose this killer action as &amp;#x2018;nanorobot driven nanomedical treatment&amp;#x2019; to combat a bacterial or viral infection. The red cells also act as imaging agent due to oxygen binding character. Our experiments were focused on the application of nanorobot (red cells) to make them immune resistant against viruses and making them as useful contrast agents in magnetic resonance imaging use based on their behavior in nanomagnetic fields. It consists of an injection of...
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4262/version/1

Biomimetic assemblage of nucleobase 5-fluorouracil into nano-size three-dimensional particles
Provedor de dados: 17
Autores: Arun Chauhan; Swaleha Zubair; Qamar Zia; Saba Tufail; Asif Sherwani; Mohammad Sajid; Mohammad Owais.
Biomimetic nano-assembly formation offers a convenient and bio friendly approach to fabricate complex structures from simple components with sub-nanometer precision. Recently, extract from cells of microorganism/plant origin have emerged as a simple and viable route for the synthesis of metal and inorganic material nano-particles. In the present study, we have extended biological synthesis of nano-particles to organic molecules, namely the anticancer agent, 5-fluorouracil (5-FU) using Aloe vera leaf extract. The formation of 5-FU nano- particles was assessed by UV, FT-IR and fluorescence spectroscopic techniques. Transmission electron microscopy and atomic force microscopic techniques confirmed nano-size of the synthesized particles. Importantly, the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6182/version/1

Biopharmaceuticals from microorganisms: from production to purification
Provedor de dados: 58
Autores: Jozala,Angela Faustino; Geraldes,Danilo Costa; Tundisi,Louise Lacalendola; Feitosa,Valker de Araújo; Breyer,Carlos Alexandre; Cardoso,Samuel Leite; Mazzola,Priscila Gava; Oliveira-Nascimento,Laura de; Rangel-Yagui,Carlota de Oliveira; Magalhães,Pérola de Oliveira; Oliveira,Marcos Antonio de; Pessoa Jr,Adalberto.
ABSTRACT The use of biopharmaceuticals dates from the 19th century and within 5-10 years, up to 50% of all drugs in development will be biopharmaceuticals. In the 1980s, the biopharmaceutical industry experienced a significant growth in the production and approval of recombinant proteins such as interferons (IFN α, β, and γ) and growth hormones. The production of biopharmaceuticals, known as bioprocess, involves a wide range of techniques. In this review, we discuss the technology involved in the bioprocess and describe the available strategies and main advances in microbial fermentation and purification process to obtain biopharmaceuticals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopharmaceuticals; Fermentation process; Biotechnology; Upstream process; Downstream process.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500051

Bio-resources for sharing: which communities and what specific needs
Provedor de dados: 17
Autores: Andres Metspalu.
Biobanks are an important source to obtain samples and data for research purposes for a wide array of communities. In fact, sharing of bio-resources and data is an integral part of a biobank&amp;#x27;s role. For an effective partnership a list of requirements or needs have to be met, assuring quality of the resources shared as well as quality of the collaboration in general. Tracking and documenting bio-resource sharing and collaborations of a biobank can be seen as one indicator of the &amp;#x22;impact&amp;#x22; a biobank has to the field. As an example, the practice of the Estonian Genome Center of the University of Tartu is presented.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5659/version/1

Biosafety education relevant to genetically engineered crops for academic and non-academic stakeholders in East Africa
Provedor de dados: 69
Autores: Sengooba,Theresa; Grumet,Rebecca; Hancock,James; Zawedde,Barbara; Kitandu,Lazaro; Weebadde,Cholani; Karembu,Margaret; Kenya,Eucharia; Meredia,Karim; Nampala,Paul; Ochanda,James O; Quemada,Hector; Rubindamayugi,Mugassa.
Development and deployment of genetically engineered crops requires effective environmental and food safety assessment capacity. In-country expertise is needed to make locally appropriate decisions. In April 2007, biosafety and biotechnology scientists, regulators, educators, and communicators from Kenya, Tanzania, and Uganda, met to examine the status and needs of biosafety training and educational programs in East Africa. Workshop participants emphasized the importance of developing biosafety capacity within their countries and regionally. Key recommendations included identification of key biosafety curricular components for university students; collaboration among institutions and countries; development of informational materials for non-academic...
Tipo: Journal article
Palavras-chave: Biotechnology; Environmental safety; Kenya; Tanzania; Uganda.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100001

BioSharing: Standards, Policies and Communication in Bioscience
Provedor de dados: 17
Autores: Dawn Field; Susanna-Assunta Sansone; Philippe Rocca-Serra.
BioSharing works at the global level to build stable linkages between journals, funders, implementing data sharing policies, and well-constituted standardization efforts in the biosciences domain, to expedite the communication and the production of an integrated standards-based framework for the capture and sharing of high-throughput genomics and functional genomic bioscience data.
Tipo: Presentation
Palavras-chave: Biotechnology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6436/version/1

Biossegurança ambiental de OGMs.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de.
ABSTRACT: In 2005, there were 90 million hectares cultivated with GMOs by 8.5 million producers in 21 countries. In 2006, we commemorated 10 years of commercial production of GMOs. Currently there are 10 million farmers planting 100 million hectares. However, several points were raised concerning the environmental impact of GMOs: (1) risk of cultivated or wild hybrid become a harmful invasive species, (2) development of resistance by the massive use of technology, (3) possibility of vertical or horizontal gene flow, (4) adverse effect on non-target and beneficial species, (5) impacts on crop production systems. This article discusses the main results on those concerns. The data obtained so far show that the environmental impacts of GMOs ares similar to a...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Transgenics; Transgenic foods.; Biotecnologia; Genética Vegetal; Melhoramento Genético Vegetal; Planta Transgênica; Segurança Alimentar.; Genetics; Food security; Biotechnology; Plant breeding.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570248

Biossegurança de alimentos transgênicos.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de.
ABSTRACT: The use of transgenic plants on the human diet is a matter of great concern for the scientific community and the public in general. However, it is important to consider that before the release for human consumption, transgenic foods are submitted to an extensive series of rigorous tests. These tests includes the characterization of the expressed protein, in vitro digestibility and oral evaluation of acute toxicity in mice, studies of the structural homology of the protein with other known toxic proteins, allergenic potential and nutritional equivalence. Questions about the true risks of transgenic food for human health cannot be answered in general terms, but we can expect a lower potential risk when compared to another type of food that did not...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Transgênico; Transgenics; Transgenic foods.; Biotecnologia; Genética Vegetal; Melhoramento Genético Vegetal; Segurança Alimentar.; Biotechnology; Food security; Genetics; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/559186

Biotechnology and Planted Forests: Assessment of Potential and Possibilities
Provedor de dados: 31
Autores: Sedjo,   Roger A.
This paper addresses the potential impact of the introduction and development of biotechnology on planted forests. It includes a description of some recent innovations in forestry including the use of traditional breeding, and also more recent innovations involving biotechnology, including the development of clonal propagation and the use of modern molecular biology techniques. In addition to describing these innovations, the paper undertakes an assessment of their probable impact on future production of the forest industry, on the global timber supply, and on future markets for timber and wood products. The paper offers a description of recent innovations in tree breeding and biotechnology, including a discussion of innovations in agriculture that have...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Plantation forests; Genetic modification; Genetic research; Economic benefits; Transgenic; GMO; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 1999
URL: http://purl.umn.edu/10862

Biotechnology and the Development of Food Markets: Retrospect and Prospects
Provedor de dados: 31
Autores: Moschini,   GianCarlo.
Biotechnology has had an important impact on the agricultural and food industries over the last twelve years by way of fast and extensive adoption of a few genetically modified (GM) crops. This has produced large efficiency gains, including higher yields and reduced costs of weed and pest control, as well as some environmental benefits. The expected development of crops with additional agronomic traits, and with output traits to improve the nutrition and health attributes of food products, holds the potential for even more pervasive impacts. Full realisation of such promises may require overcoming the constraining effects of restrictive GM product regulations.
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Genetically modified products; Innovation; Regulation; Research and development.
Ano: 2008
URL: http://purl.umn.edu/42811

Biotechnology applied to salmoniculture
Provedor de dados: 96
Autores: Isler,Iván Valdebenito.
This work presents a conceptual analysis of the main biotechnologies used in Chilean salmoniculture, which is based on the production of Atlantic salmon (Salmo salar, 356.407 t),silver salmon or coho (Oncorhynchus kisutch, 116.481 t), (O. tshawytscha, 2.062 t) and rainbow trout (O. mykiss, 189.178 t).These activities are focused on the photoperiod artificial manipulation to obtain out-of-season spawning, in the use of hormonal therapies which allow synchronizing the final oocyte maturation (FOM) and sexual maturity acceleration or the increase in milt volume produced by males. Such actions are carried out using GnRHa in doses close to 10 µg/kg of fish. Once sexual maturity is reached, in vitro manipulation of gamets must often be done due to either the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Induced spawning; Photoperiod; Reproduction; Sex manipulation; Thermoperiod.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300004

BIOTECHNOLOGY IN AGRICULTURE: IMPLICATIONS FOR FARM-LEVEL RISK MANAGEMENT
Provedor de dados: 31
Autores: Makki,   Shiva S.; Somwaru,   Agapi; Harwood,   Joy L.
This study examines the risks associated with adoption of biotech crops and discusses their implications for risk management at the farm level. We develop an analytical risk evaluation matrix framework to illustrate changes in production and marketing risks of biotech and non-biotech crops. Price uncertainty generated by consumer concerns is the major risk facing biotech farmers, while cross-pollination with biotech crops and preservation of non-biotech status are major concerns for non-biotech farmers. Improved market infrastructure to handle biotech products and modification of the current risk management tools to accommodate new risks are essential in reducing the farm-level risks.
Tipo: Journal Article
Palavras-chave: Biotechnology; Genetically modified organisms; Marketing risks; Production risks; Risk evaluation matrix; Risk management; Research and Development/Tech Change/Emerging Technologies; Risk and Uncertainty.
Ano: 2001
URL: http://purl.umn.edu/14683

BIOTECHNOLOGY REGULATIONS AND THE WTO
Provedor de dados: 31
Autores: Sheldon,   Ian M.; Josling,   Timothy E.
This paper examines the regulation of trade in genetically modified organisms (GMOs) by the World Trade Organization (WTO). Despite rapid adoption of GMOs by a few exporters, many importers have developed relatively restrictive procedures for pre-market approval of GMOs, and are introducing mandatory labeling. While exporters have yet to seek a ruling from the WTO on these regulations, a trade dispute over GMOs is likely to occur before too long. Exporting countries will likely argue that importing countries' regulations are too restrictive, given existing scientific knowledge of the safety of current GM crops, and that labeling of GM foods is unnecessary due to the fact that they are typically similar to their conventional counterparts. In response,...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Regulation; Trade; WTO; International Relations/Trade; Research and Development/Tech Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/14594

Biotechnology's Potential Contribution to Global Wood Supply and Forest Conservation
Provedor de dados: 31
Autores: Sedjo,   Roger A.
Over the past 30 years, industrial plantation forests have become a major supplier of industrial wood. There are several reasons for this, including the improved economics of planted forests due to biotechnological innovations, the increases in natural forest wood costs due to increasing inaccessibility, and rising wood costs from natural forests due to new environmental restrictions related to logging. Forestry today is on the threshold of the widespread introduction of biotechnology into its operational practices. In many cases, the biotechnology likely to be introduced is simply an extension of that being utilized in agriculture, such as herbicide-tolerant genes. However, biotechnology in forestry also is developing applications unique to forestry,...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Breeding; Forestry; Tree plantations; Timber; Fiber; Genes; GMOs; Industrial wood; Economics; Benefits; Costs; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 2001
URL: http://purl.umn.edu/10708

Biotech--Who Wins? Economic Benefits and Costs of Biotechnology Innovations in Agriculture
Provedor de dados: 31
Autores: Moschini,   GianCarlo.
The conceptual model necessary for an assessment of biotechnology's economic benefits and costs is outlined, emphasizing the need to account for the proprietary nature of biotechnology innovations. The model is illustrated with an application to Roundup Ready soybeans. The estimated value of this innovation is sizeable, with consumers and innovators claiming the largest shares of net benefits. Also, disparities in intellectual property rights protection across countries affect the distribution of benefits. Consumer resistance toward GMOs and the issue of labelling and market segregation complicate the economic evaluation of biotechnology innovations; a number of related regulation and public policy issues are discussed. Emerging output-trait GMOs are...
Tipo: Journal Article
Palavras-chave: Biotechnology; Genetically modified organisms; Identity preservation; Intellectual property rights; R&amp;D; Transgenic crops; Welfare evaluation; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/23862

Biotecnologia: Perfil dos Grupos de Pesquisa no Brasil
Provedor de dados: 31
Autores: Mendonca,   Marco Aurelio Alves de; Freitas,   Rogerio Edivaldo.
O artigo objetiva mapear o perfil do capital humano envolvido com a biotecnologia no Brasil. Empregou-se análise estatística descritiva. A base de dados foi exclusivamente montada para esta pesquisa a partir de informações do Portal de Inovação, do Ministério da Ciência e Tecnologia. A investigação constatou a existência de importante incremento dos grupos de pesquisa relacionados ao tema, nos últimos anos. Tal fato resulta dos esforços empreendidos por uma série de agentes, sendo o governo federal o principal. Ademais, os dados indicam que os pesquisadores concentram-se especialmente nas regiões Sul e Sudeste, com significativa participação das ciências Agrárias, Biológicas e da Saúde.--------------------The article aims to map the human capital profile...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotecnologia; Capital Humano; Análise Descritiva; Biotechnology; Human Capital; Descriptive Analysis; Labor and Human Capital; Research and Development/Tech Change/Emerging Technologies.
Ano: 2008
URL: http://purl.umn.edu/108141

Biotecnologias aplicadas à reprodução de ovinos e caprinos.
Provedor de dados: 14
Autores: FONSECA, J. F. da; CRUZ, R. do C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G. de; VIANA, J. H. M.
A criação de caprinos e ovinos é uma atividade agropecuária de elevada importância social, econômica e consolidada em determinadas regiões do Brasil, enquanto em outras, mostra-se em fase emergente. Diversos aspectos apontam para o desenvolvimento desta cadeia produtiva no país. Dentre eles, a produção nacional, especialmente de carne e leite, sequer abastece o mercado interno, mesmo considerando que o consumo ainda é reduzido em relação a outros países. Em função da qualidade e diferencial dos produtos de origem caprina e ovina, acredita-se que em pouco tempo a demanda aumentará expressivamente, requerendo sistemas de criação focados na produtividade. Assim, torna-se fundamental desenvolver e adequar tecnologias voltadas ao incremento produtivo. Para...
Tipo: Livro técnico (INFOTECA-E)
Palavras-chave: Transferência nuclear; Cloning.; Biotecnologia; Caprino; Clonagem; Inseminação artificial; Ovino; Reprodução animal; Sêmen.; Goats; Biotechnology; Reproduction; Sheep; Transgenic animals.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1027956

Biotransformation of artemisinin mediated through fungal strains for obtaining derivatives with novel activities
Provedor de dados: 17
Autores: Suchita  Srivastava; Suaib  Luqman; Atiya  Fatima; Mahendra  P. Darokar; Arvind  S. Negi; J K. Kumar; Karuna  Shanker; Chandan  S. Chanotiya; Sudeep  Tandon; Suman  P. S. Khanuja.
Artemisinin, a sesquiterpene lactone, is the active antimalarial constituent of Artemisia annua. Several fungal strains Saccharomyces cerevisiae, Aspergillus flavus, Aspergillus niger and Picchia pastoris were used to biotransform artemisinin. Among these strains, A. flavus was the only microorganism capable of transforming artemisinin to deoxyartemisinin in higher yields than the previous reports. The structure of deoxyartemisinin was elucidated by spectroscopy. Deoxyartemisinin showed antibacterial activity against Staphylococcus aureus, S. epidermidis and S. mutans at a minimum inhibitory concentration (MIC) of 1 mg/mL compared to artemisinin whose MIC was &amp;#x3e;2 mg/mL.
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Microbiology; Molecular Cell Biology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6925/version/1

Blueberry production in Chile: current status and future developments
Provedor de dados: 94
Autores: Retamales,Jorge B.; Palma,María J.; Morales,Yohanna A.; Lobos,Gustavo A.; Moggia,Claudia E.; Mena,Carlos A.
Chile has become a major actor in the blueberry industry as the most important supplier of off-season fresh fruit for the northern hemisphere. Blueberry exports passed from US$ 30 million (around 4,000 tons) in 2000 to US$ 380 million (94,000 tons) in 2011. The characteristics of the major blueberry growing regions (North, Central, South-central and South) are presented in terms of acreage, varieties, management practices, extension of the harvest season, and soil and climatic conditions. Most fruit is from highbush varieties, picked by hand and exported fresh by boat to United States. Largest proportion of fruit is exported from mid December to late January, which coincides with lowest prices. The south-central region (latitudes 34º50' to 38º15' S) was in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Growth regulator and plant science.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000100008

Bogoch Replikins Pandemic Prevention: Increase of Strain-Specific Influenza Genomic Replikin Counts, Having Predicted Outbreaks and their Location Seven Times Consecutively, Up to Two Years in Advance, Provides Time for Prevention of Pandemics
Provedor de dados: 17
Autores: Samuel     Bogoch; Elenore S. Bogoch.
Earlier studies have shown that the increased concentration of a new class of virus genomic peptides, Replikins, precedes and predicts virus outbreaks. We now find that the area in the genome of the highest concentration of Replikins, and the country in which this peak exists in scout viruses, have permitted in the past five years seven consecutive accurate predictions of the geographic localization of coming outbreaks, including those now realized in Mexico for H1N1, and in Cambodia for H5N1. Real-time Replikin analysis of the evolution of the virus genome identified both mutations and structural reorganization of the hemagglutinin and p B1 genes over several years before each outbreak. This information, together with the specific Replikin sequences so...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6952/version/1

Botânica: uma ciência básica ou aplicada?
Provedor de dados: 88
Autores: AZEVEDO,JOÃO LÚCIO.
Exemplos de interações entre microrganismos endofíticos e suas plantas hospedeiras são usados para demonstrar a importância da Botânica no desenvolvimento da biotecnologia e para a preservação da biodiversidade. Como conclusão, uma visão holística da ciência atual torna irrelavante qualquer distinção entre aspectos básicos e aplicados da ciência, principalmente quando se consideram as ciências biológicas, como é o caso da Botânica.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Biodiversity; Plant-microbes interactions; Endophytes.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041999000500002

BRAZIL: THE FUTURE OF MODERN AGRICULTURE?
Provedor de dados: 31
Autores: Matthey,   Holger; Fabiosa,   Jacinto F.; Fuller,   Frank H.
In an attempt to understand better Brazil'’s future role in agricultural markets, the authors of this report traveled to Brazil on a fact-finding mission in September 2003. The goal was to get a first-hand impression of Brazil’'s agricultural sector and especially its future potential. In this report we provide a general description of crop and livestock production, government policies, public and private cooperation, and transportation and biotechnology issues. The most striking observations made during our time in Brazil was the universal sense of optimism expressed by producers, government officials, agronomists, and market analysts about the future growth of Brazilian agricultural production. Does that mean Brazil, as one person suggested, is the...
Tipo: Working or Discussion Paper
Palavras-chave: Agricultural development; Agricultural policy; Biotechnology; Infrastructure; Brazil; Production; Agricultural and Food Policy; International Development; Production Economics; Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/18705

BRIF Workshop Introduction
Provedor de dados: 17
Autores: Anne Cambon-Thomsen.
This presentation aims at introducing the place of BRIF in the landscape of data sharing, the BRIF concept, a presentation of the working group, the ongoing debates and the workshop objectives.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5658/version/1

Building Block and Building Rule: Dual Descriptor Method for Biological Sequence Analysis
Provedor de dados: 17
Autores: Bin-Guang Ma.
The emergence of &amp;#x201c;Systems Biology&amp;#x201d; in recent years highlights the systematic viewpoint of bio-system modeling. Building on such a background, Dual Descriptor Method, a generic methodology for biological sequence analysis is proposed. From a systematic perspective, Dual Descriptor is defined as a two element set of Composition Weight Map and Position Weight Function which aim at reflecting the composition and permutation information of a sequence. An alternate training algorithm is provided to get an optimum description of the building patterns of the sequences. In this paper, dual descriptor method has been applied to the analysis of two typical problems of molecular biology: gene identification and the prediction of protein function....
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2223/version/1

Caminhos da Biotecnologia da Reprodução em Suínos.
Provedor de dados: 14
Autores: SOUZA, A. P. de; SILVA, Z.; SUPERTI, B. F. V.; MARQUES, M. G.
Artigo descreve algumas das biotecnologias que estão aprimoradas e podem abrir novas possibilidades para a reprodução na espécie suína.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Suinocultura; Biotecnologia; Reprodução animal; Animal reproduction; Swine; Biotechnology.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1061837

Can bacteria adapt to starvation-free environment?
Provedor de dados: 17
Autores: Kei Kitahara.
Bacteria will experience starvation-free environment if infinite nutrition is supplied continuously for a long period. In this study, an evolutionary experiment was performed for 118 days where bacteria adapted to starvation-free environment and reduced their doubling time. It is anticipated that this finding will help to select bacterial strains that can grow more rapidly in rich media.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7017/version/1

Can Technology Deliver on the Yield Challenge to 2050?
Provedor de dados: 31
Autores: Fischer,   R.A.; Byerlee,   Derek R.; Edmeades,   Gregory O.
This paper was produced for the FAO Expert Meeting on How to Feed the World in 2050 (Rome, 24-26 June 2009).
Tipo: Conference Paper or Presentation
Palavras-chave: Technology; Productivity; Yield gaps; Investment in R&amp;D; Biotechnology; Input efficiency; Research and Development/Tech Change/Emerging Technologies.
Ano: 2009
URL: http://purl.umn.edu/55481

Cancer Clinical Trials Optimization and Pharmacogenomics
Provedor de dados: 17
Autores: I. C. Baianu.
A critical overview of recent clinical trials in cancer is presented focused on signaling pathways blockers or inhibitors with a view to developing successful clinical trials employing personalized cancer therapies. Rational, pharmacogenomic strategies in cancer trials should be adopted that include specific molecular targeting based on adequate data for, and detailed modeling of, cancer cell genomes, modifications of cancer signaling pathways and epigenetic mechanisms. Novel translational oncogenomics research is rapidly expanding through the application of highly sensitive and specific advanced technology, research findings and computational tools and complex models to both pharmaceutical and clinical problems. Multiple sample analyses from several...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7046/version/1

Cancer invasion associated gene expression signature is present in differentially expressed genes in the reprogramming of fibroblasts into stem cells
Provedor de dados: 17
Autores: Wei-yi Cheng; Hoon Kim; Jessica Kandel; Dimitris Anastassiou.
Tumors become invasive by penetrating adjacent connective tissue, but the underlying biological mechanisms remain obscure. We recently identified a precise gene expression signature of fibroblastic origin associated with cancer invasion, the first step of the metastatic cascade. The signature contains many coordinately overexpressed genes, prominent among which are COL11A1, THBS2 and INHBA. Here we show that there is a striking similarity between the set of expressed genes in this metastasis-associated fibroblastic (MAF) signature and the set of genes that are downregulated when fibroblasts are reprogrammed to induced pluripotent stem cells (iPSCs). Because it is known that fibroblast reprogramming involves a mesenchymal epithelial transition (MET), the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5924/version/1

Caracterizacao molecular e estabelecimento de colecao de trabalho de Stylosanthes macrocephala com base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; BARROS, A. M.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.; GOMES, A. C.; ANDRADE, R. P.
bitstream/CPAC-2009/24949/1/p2004_03.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: RAPD; Feed legumes.; Biologia Molecular; Biotecnologia; Marcador Molecular; Leguminosa Forrageira; Variação Genética.; Genetic markers; Genetic variation; Biotechnology; Molecular biology.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567912

Carbon Orientation in the Diatom Phaeodactylum tricornutum: The Effects of Carbon Limitation and Photon Flux Density
Provedor de dados: 5
Autores: Heydarizadeh, Parisa; Veidi, Brigitte; Huang, Bing; Lukomska, Ewa; Wielgosz-collin, Gaetane; Couzinet-mossion, Aurélie; Bougaran, Gael; Marchand, Justine; Schoefs, Benoit.
Diatoms adapt to changing environmental conditions in very efficient ways. Among the mechanisms that can be activated, the reorientation of carbon metabolism is crucial because it allows the storage of energy into energy-dense molecules, typically lipids. Beside their roles in physiology, lipids are commercially interesting compounds. Therefore studies dealing with this topic are relevant for both basic and applied science. Although the molecular mechanisms involved in the reorientation of carbon metabolism as a response to a deficiency in nutrients such as nitrogen or phosphorus has been partially elucidated, the impacts of carbon availability on the implementation of the reorientation mechanisms remain unclear. Indeed, it has not been determined if the...
Tipo: Text
Palavras-chave: Diatom; Carbon deficiency; Carbon metabolism; Stress; Light intensity; Regulation; Biotechnology; Phosphoenolpyruvate.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00489/60058/63338.pdf

Category of Metabolic-Replication Systems in Biology and Medicine
Provedor de dados: 17
Autores: I. C. Baianu.
Metabolic-repair models, or (M,R)-systems were introduced in Relational Biology by Robert Rosen. Subsequently, Rosen represented such (M,R)-systems (or simply MRs)in terms of categories of sets, deliberately selected without any structure other than the discrete topology of sets. Theoreticians of life&amp;#x27;s origins postulated that Life on Earth has begun with the simplest possible organism, called the primordial. Mathematicians interested in biology attempted to answer this important question of the minimal living organism by defining the functional relations that would have made life possible in such a minimal system- a grandad and grandma of all living organisms on Earth.
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Neuroscience; Bioinformatics; Earth &amp; Environment; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7045/version/1

Cattle farmers: profile and speech content analysis while undergoing training to adopt artificial insemination in Goiás State, Brazil
Provedor de dados: 96
Autores: Gordo,João Maurício Lucas; Silva,Marcelo Corrêa da; Solano,Gianice de Almeida; Lopes,Fernando Brito; Costa,Marcos Fernando Oliveira e; Rocha,Francisco Eduardo de Castro; Fioravanti,Maria Clorinda Soares; Sereno,José Robson Bezerra.
This study was undertaken to verify major issues present in the discourse of cattle farmers when discussing the use of artificial insemination (AI). Seventy-one beef and dairy cattle farmers in the state of Goiás, Brazil, were interviewed using semi-structured questionnaires during AI training courses in 2010 and 2011. Data analyses were done using the classic method of textual speech content analysis, performing the pre-analytical, analytical and inferential stages. The written questions were separated in elementary context units (ECU) and sorted into similar thematic groups. These groups originated categories and subcategories, which were nominated based on an a posteriori criteria. Overall, personal motivation was the main issue characterizing discourse...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal reproduction; Biotechnology; Discourse analysis; Social psychology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000300003

Causal Stability Ranking
Provedor de dados: 17
Autores: Daniel J. Stekhoven; Lars Hennig; Gardar Sveinbj&amp;#xf6;rnsson; Izabel Moraes; Marloes H. Maathuis; Peter B&amp;#xfc;hlmann.
Genotypic causes of a phenotypic trait are typically determined via randomized controlled intervention experiments. Such experiments are often prohibitive with respect to durations and costs. We therefore consider inferring stable rankings of genes, according to their causal effects on a phenotype, from observational data only. Our method allows for efficient design and prioritization of future experiments, and due to its generality it is useable for a broad spectrum of applications.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6716/version/1

Cell Proteomic Footprint
Provedor de dados: 17
Autores: Petr Lokhov; Elena Balashova; Maxim Dashtiev.
The authentication of mammalian cell cultures and their subpopulations are of tremendous demand in biotechnology and cell therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low sample cost, and high sensitivity. We discovered that cell cultures treated with protease at soft, &amp;#x201c;non-killing&amp;#x201d; conditions release fragments of cell surface proteins, which composition is a strong characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass spectrum with the mass spectrum of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2091/version/1

Cell-free Embryonic Stem Cell Extract-mediated Derivation of Multi-potent Stem Cells from NIH3T3 Fibroblasts for Functional and Anatomical Ischemic Tissue Repair
Provedor de dados: 17
Autores: Johnson Rajasingh; Hiromichi Hamada; Evelyn Bord; Tina Thorne; Ilona  Goukassian; Erin  Lambers; Prasanna Krishnamurthy; Gangjian  Qin; Karen Schlauch; Kenneth M. Rosen; Deepali Ahluwalia; Yan  Zhu; Douglas W. Losordo; Raj Kishore.
The oocyte-independent generation of multipotent stem cells is one of the goals in regenerative medicine. We report that upon exposure to mouse ES cell (ESC) extracts, reversibly permeabilized NIH3T3 cells undergo de-differentiation followed by stimulus-induced re-differentiation into multiple lineage cell types. Genome-wide expression profiling revealed significant differences between NIH3T3 and ESC-extract treated NIH3T3 cells including re-activation of ESC specific transcripts. Epigenetically, ESC extracts induced CpG de-methylation of Oct4 promoter, hyper-acetylation of histones 3 and 4 and decreased lysine 9 (K-9) dimethylation of histone 3. In mouse models of surgically-induced hind limb ischemia (HLI) or acute myocardial infarction (AMI)...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1208/version/1

Cellular delivery of antibodies: effective targeted subcellular imaging and new therapeutic tool
Provedor de dados: 17
Autores: Marzia  Massignani; Irene Canton; Nisa Patikarnmonthon; Nicholas J. Warren; Steven P. Armes; Andrew L. Lewis; Giuseppe Battaglia.
It is already more than a century since the pioneering work of the Nobel Laureate Ehrlich gave birth to the side chain theory1, which helped to define antibodies and their ability to target specific biological sites. However, the use of antibodies is still restricted to the extracellular space due to the lack of a suitable delivery vehicle for the efficient transport of antibodies into live cells without inducing toxicity. In this work, we report the efficient encapsulation and delivery of antibodies into live cells with no significant loss of cell viability or any deleterious affect on the cell metabolic activity. This delivery system is based on poly(2-(methacryloyloxy)ethyl phosphorylcholine)-block-(2-(diisopropylamino)ethyl methacrylate), (PMPC-PDPA),...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Immunology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4427/version/1

CENARGEN, función e importancia.
Provedor de dados: 14
Autores: VALOIS, A. C. C.
Tipo: Artigo de periódico
Palavras-chave: CENARGEN; Biotecnologia; Instituição de Pesquisa; Recurso Genético; Biotechnology; Genetic resources; Research institutions.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/171558

CFP and YFP photostabilities are differentially affected by common mounting fluids
Provedor de dados: 17
Autores: Johannes A. Schmid.
The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking and tracing of specific proteins or cells or to determine protein interactions. In the later case, the quantum physical phenomenon of fluorescence resonance energy transfer (FRET) is visualized by specific microscopy techniques. When we applied a commonly used FRET microscopy technique - the increase in CFP-fluorescence after bleaching of YFP, we noticed that it worked well for live cells, but that most of the FRET-signal was lost in fixed cells mounted in commercial microscopy mounting fluids. Subsequently, we could show that CFP bleached much faster...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4517/version/1

Changes in Antioxidant Enzymes Activity and Oxidative Damage in Four Argania spinosa Ecotypes Under Water Stress Conditions
Provedor de dados: 17
Autores: Abdelghani Chakhchar; Abderrahim  Ferradous; Mouna   Lamaoui; Said Wahbi; Cherkaoui  El Modafar.
_Argania spinosa_ L. Skell, a tree endemic of Morocco, grows in semiarid and arid areas. Biochemical response to drought in four ecotypes of the _A. spinosa_ was compared. Choice of ecotypes was based on evident differences in geographical features in south-west Morocco: two coastal ecotypes (Essaouira and Agadir) and two paralittorale ecotypes (Aoulouz and Lakhssas). Lipid peroxidation (malonyldialdehyde (MDA)), content of endogenous hydrogen peroxide (H2O2) and antioxidant enzyme system (Catalase (CAT), Ascorbate peroxidase (APX), Total peroxidase (POD) and Polyphenoloxidase (PPO) were measured in control (100% relative humidity (RH) at field capacity (FC)) and water-stressed plants (50 and 25% RH at FC). For all ecotypes, drought stress significantly...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6189/version/1

Chapter 07: USING CONJOINT ANALYSIS TO ASSESS CONSUMERS' ACCEPTANCE OF PST-SUPPLEMENTED PORK
Provedor de dados: 31
Autores: Halbrendt,   Catherine K.; Pesek,   John D., Jr.; Parsons,   April; Lindner,   Robert K.
This book was originally published by Westview Press, Boulder CO, 1995.
Tipo: Book Chapter
Palavras-chave: Biotechnology; Pork products; Conjoint analysis; Willingness to buy; Food Consumption/Nutrition/Food Safety.
Ano: 1995
URL: http://purl.umn.edu/25973

Characterization of a pig skeletal muscle microarray to study pork quality: the GenmascqChip 15K
Provedor de dados: 17
Autores: Marie Damon; Fr&amp;#xe9;d&amp;#xe9;ric Herault; Annie Vincent; Pascale Le Roy; Pierre Cherel.
Pork quality is highly variable and availability of relevant predictors of fresh meat quality at slaughter is critical to optimize carcass use and improve its valuation. High throughput gene expression studies have been widely used to describe biological mechanisms underlying variation of several traits but data are scarce regarding their use as tools for development of biomarkers. The GENMASCQ (GENomics enabled Marker Assisted Selection and Certification of Quality in Pork products ) research program was set up to select, using high-throughput gene expression screening, markers of the biological variation mostly appropriate to describe, and ultimately forecast, the sensory quality of an important meat product, accounting for nearly 40 % of meat protein...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5730/version/1

Chickpea Genomics Consortium on Drought Tolerance
Provedor de dados: 30
Autores: Varshney, R.K.,( Rajeev K. Varshney ).
Palavras-chave: Genomes; Chickpeas; Biotechnology; Genes; Tolerance; Weather hazards.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3324

CHINA'S FOOD AND AGRICULTURE: ISSUES FOR THE 21ST CENTURY
Provedor de dados: 31
China's impact on world agricultural markets in coming decades will depend on many factors. Growing income and transition to modern urban lifestyles will increase demand for all foods, but demand will shift toward meat and high-value products. WTO accession may increase openness of China's agricultural trade and allow more imports. An understanding of geographic variations of consumption, production, trade, and policy is critical to understanding the vast China market. Development of transportation infrastructure and market channels will make it easier for food products to reach consumers. China's approach to biotechnology and its reform of institutions for allocating land, labor, and water inputs have important implications for agricultural productive...
Tipo: Report
Palavras-chave: China; Food; Agriculture; Production; Consumption; Regions; International trade; Biotechnology; Livestock; Land; Irrigation; Retail; Transportation; Marketing; Rural development; Labor markets; Statistics; Agricultural and Food Policy.
Ano: 2002
URL: http://purl.umn.edu/33723

CHINA'S REGULATION OF BIOTECHNOLOGY - DOES IT CONFORM TO THE WTO?
Provedor de dados: 31
Autores: Loppacher,   Laura J.; Kerr,   William A.
The People's Republic of China has been working for over two decades to develop a domestic biotechnology industry. Even though China is a developing country, it is set to become a significant international player in the field of biotechnology. China's accession to the World Trade Organization in December of 2001 means that China's trade and regulatory regime should now reflect its WTO commitments. This paper outlines the development of the Chinese biotechnology industry and how it may evolve in the future. It assesses China's current approach to trade and regulation of biotechnology in relation to GATT rules on trade in goods and the TRIPS commitments pertaining to the protection of intellectual property. The conclusion is that China's trade and...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; China; Intellectual property; WTO; International Relations/Trade.
Ano: 2004
URL: http://purl.umn.edu/23938

Chloroplast and Cell Imaging at Submicron Resolution by Two-Photon Excitation
Provedor de dados: 17
Autores: I C. Baianu.
Novel, two-photon NIR excitation fluorescence correlation microspectroscopy tests and preliminary results were presented in this article with submicron resolution for concentrated suspensions of functional cells and chloroplast membranes. Related developments of these technique involve applications of Fluorescence Cross-Correlation Spectroscopy (FCCS) detection to monitoring: &amp;#xd;&amp;#xa;DNA- telomerase interactions, DNA hybridization kinetics, ligand-receptor interactions, and HIV-HBV testing. &amp;#xd;&amp;#xa;
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Bioinformatics; Plant Biology; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7116/version/1

Clean Coal and Spirulina maxima (Cyanobacteria) Production through Alkaline-Seawater
Provedor de dados: 17
Autores: Md. Abu Affan; Dae-Won  Lee; Heung-Sik Park; Jae-Hoon Noh; Soo-Jin Heo; Chulhong Oh; Seon-Mi Jeon; Joo-Won Son; Seung-Gyu Son; Hyi-Seung Lee; Hyeon Yong Lee; Do-Hyung Kang.
Carbon capture and storage have involved high cost and an earthquake threat. Coal in NaOH-natural seawater (NSW) can produce clean coal, and organic carbon enriched pretreated sweater (PSWS) can be used to grow Spirulina maxima. However, with freshwater shortage, high cost CO2 feedstock incurs S. maxima biomass production, and using PSWS should be considered as an alternative. NaHCO3 and Na2CO3 were used to sediment Mg2+ and Ca2+ of NSW to resolve turbidity and decreasing phosphate solubility. Here, we show that pre-combustion coal and NaOH concentration calculated by our devised formulae for any salinity could dilute volatile matter 19.8 Kg plus CO2 116.50 to 168.89 Kg Ton^-1^ coal in NaOH-NSW by sedimentation Mg2+ and Ca2+ of 99.91% and 32.52%,...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6930/version/1

Cloning and expression of first gene for biodegrading microcystins by Sphingopyxis sp. USTB-05
Provedor de dados: 17
Autores: Hai Yan; Junfeng Wang; Jian Chen; Wei Wei; Hui Wang.
Harmful cyanobacterial blooms (HCBs) in natural waters are a growing environmental problem worldwide because microcystins (MCs) produced by cyanobacteria are potent hepatotoxins and tumor promoters. MCs are resistant against physical and chemical factors. Thus, biodegradation is the most efficient method for removing MCs, and a number of bacterial strains, especially genus _Sphingomonas_, have been isolated for biodegrading MCs. Although the pathway, enzyme, and gene for biodegrading MCs by _Sphingomonas sp._ have been widely identified recently, no gene concerned with the biodegradation of MCs has been successfully cloned and expressed. In this study, we show that the first and most important gene of mlrA, containing 1,008 bp nucleotides in length, in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Earth &amp; Environment.
Ano: 2010
URL: http://precedings.nature.com/documents/4846/version/1

CloVR-16S: Phylogenetic microbial community composition analysis based on 16S ribosomal RNA amplicon sequencing &amp;#x2013; standard operating procedure, version1.0
Provedor de dados: 17
Autores: James R. White; Cesar Arze; Malcolm Matalka; The CloVR Team; Samuel V. Angiuoli; W. Florian Fricke.
The CloVR-16S pipeline employs several well-known phylogenetic tools and protocols for the analysis of 16S rRNA sequence datasets:&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;A) Mothur [1] &amp;#x2013; a C++ - based software package used for clustering 16SrRNA sequences into operational taxonomic units (OTUs). Mothur creates OTUs using a matrix that describes pairwise distances between representative aligned sequences and subsequently estimates within-sample diversity (alpha diversity);&amp;#xd;&amp;#xa;B)The Ribosomal Database (RDP) naive Bayesian classifier [2] assigns each 16S sequence to a reference taxonomy with associated empirical probabilities based on oligonucleotide frequencies;&amp;#xd;&amp;#xa;C) Qiime [3] &amp;#x2013; a python-based workflow package,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5888/version/1

CloVR-Metagenomics: Functional and taxonomic microbial community characterization from metagenomic whole-genome shotgun (WGS) sequences &amp;#x2013; standard operating procedure, version 1.0
Provedor de dados: 17
Autores: James R. White; Cesar Arze; Malcolm Matalka; The CloVR Team; Samuel V. Angiuoli; W. Florian Fricke.
The CloVR-Metagenomics pipeline employs several well-known tools and protocols for the analysis of metagenomic whole-genome shotgun (WGS) sequence datasets:&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;A) UCLUST [1] &amp;#x2013; a C++-based software package for clustering redundant DNA sequences and removing artificial 454 replicates [1]; &amp;#xd;&amp;#xa;B) BLASTX and BLASTN [2] for functional and taxonomic assignment of sequences, respectively;&amp;#xd;&amp;#xa;C) Metastats [3] and custom R scripts to generate additional statistical and graphical evaluation.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The CloVR-Metagenomics pipeline accepts as input multiple fasta files (1 sample per file) and a corresponding tab-delimited metadata file that specifies features associated...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5886/version/1

CloVR-Microbe: Assembly, gene finding and functional annotation of raw sequence data from single microbial genome projects &amp;#x2013; standard operating procedure, version 1.0
Provedor de dados: 17
Autores: Kevin Galens; James R. White; Cesar Arze; Malcolm Matalka; Michelle Gwinn Giglio; The CloVR Team; Samuel V. Angiuoli; W. Florian  Fricke.
The CloVR-Microbe pipeline performs the basic processing and analysis steps required for standard microbial single-genome sequencing projects: A) Whole-genome shotgun sequence assembly; B) Identification of protein and RNA-coding genes; and C) Functional gene annotation. B) and C) are based on the IGS Annotation Engine (http://ae.igs.umaryland.edu/), which is described elsewhere (K Galens et al. submitted). The assembly component of CloVR-Microbe can be executed independently from the gene identification and annotation components. Alternatively, pre-assembled sequence contigs can be used to perform gene identifications and annotations. The pipeline input may consist of unassembled raw sequence reads from the Sanger, Roche/454 GS FLX or Illumina GAII or...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5887/version/1

Cocaine self-administration in the mouse: A low-cost, chronic catheter preparation
Provedor de dados: 17
Autores: Matthew B. Pomrenze; Michael  V. Baratta; Brian A. Cadle; Donald C. Cooper.
Intravenous drug self-administration is the most valid animal model of human addiction because it allows volitional titration of the drug in the blood based on an individual&amp;#x2019;s motivational state together with the pharmacokinetic properties of the drug. Here we describe a reliable low-cost mouse self-administration catheter assembly and protocol that that can be used to assess a variety of drugs of abuse with a variety of protocols. We describe a method for intravenous catheter fabrication that allows for efficient and long-lasting intravenous drug delivery. The intravenous catheters remained intact and patent for several weeks allowing us to establish stable maintenance of cocaine acquisition. This was followed by a dose response study in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/7040/version/1

Co-detection: Ultra-reliable Nanoparticle-Based Electrical Detection of Biomolecules in the Presence of Large Background Interference
Provedor de dados: 17
Autores: Yang Liu; Evangelyn Alocilja; Shantanu Chakrabartty.
Contrary to popular belief, we report that background interference in conductimetric biochips can be exploited using a novel &amp;#x22;co-detection&amp;#x22; principle to significantly improve the reliability of detecting trace quantities of biomolecules. The technique called &amp;#x22;co-detection&amp;#x22; exploits the non-linear redundancy amongst synthetically patterned biomolecular logic circuits for deciphering the presence or absence of target biomolecules in a sample. In this paper, we demonstrate the &amp;#x22;co-detection&amp;#x22; principle on gold-nanoparticle based conductimetric soft-logic circuits which uses a silver-enhancement technique for signal amplification. Using co-detection, we have been able to measure a 1000 times improvement in...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4389/version/1

Coincidence between transcriptome analyses on different microarray platforms using a parametric framework
Provedor de dados: 17
Autores: Tomokazu Konishi; Fumikazu Konishi; Shigeru Takasaki; Kohei Inoue; Kouji Nakayama; Akihiko Konagaya.
A parametric framework for the analysis of transcriptome data is demonstrated to yield coincident results when applied to data acquired using two different microarray platforms. Discrepancies among transcriptome studies are frequently reported, casting doubt on the reliability of collected data. The inconsistency among observations can be largely attributed to differences among the analytical frameworks employed for data analysis. The existing frameworks normalizes data against a standard determined from the data to be analyzed. In the present study, a parametric framework based on a strict model for normalization is applied to data acquired using an in-house printed chip and GeneChip. The framework is based on a common statistical characteristic of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1746/version/1

Comments and Suggestions for Improvement of the  Archon Genomics X PRIZE Validation Protocol
Provedor de dados: 17
Autores: Alexander Wait Zaranek; Tom Clegg; Ward  Vandewege; Joseph V. Thakuria.
This document is a comment on the X PRIZE validation protocol written by Kedes et al. (2011).  We propose several modifications which we think will improve the fairness and transparency of the contest while keeping the cost of the validation process under control.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5766/version/1

Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea
Provedor de dados: 5
Autores: Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul.
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins...
Tipo: Text
Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf

Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts from Alternanthera maritima (Amaranthaceae)
Provedor de dados: 58
Autores: Salvador,Marcos J.; Pereira,Paulo S.; França,Suzelei C.; Candido,Regina C.; Ito,Izabel Y.; Dias,Diones A.
The aim of this study was to evaluate the antibacterial and antifungal activity of callus culture (two different hormonal combination culture medium) and adult plants (two collect) extracts from Alternanthera maritima (Amaranthaceae) investigating the maintenance of antimicrobial activity in vivo and in vitro. The antibacterial and antifungal activity was determined by the agar-well diffusion method against thirty strains of microorganisms including Gram-positive and Gram-negative bacteria, yeasts and dermatophytes. All the organic crude extracts studied were bioactive. Extracts of aerial parts and roots of adult plants collected during the same period of years of 1995 and 1998 (Restinga de Maricá (RJ), collect 1 and 2) inhibited the growth of several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternanthera maritima; Adult plants; Callus culture; Biotechnology; Antimicrobial activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100022

Comparing Consumer Attitudes towards Genetically Modified Food in Europe
Provedor de dados: 31
Autores: Springer,   A.; Mattas,   Konstadinos; Papastefanou,   G.; Tsioumanis,   Asterios.
As biotechnology evolves new methods of genetic engineering are now being applied to the production and processing of foods. This paper is trying to explore the attitudes of the European consumers towards genetic modification of food. Using survey data of the EU member countries the proposed research paper is planned to have a threefold output: 1) providing a comparative ranking of the EU member countries in relation to the prevalence of rejection of genetically modified food, 2) uncovering intra-european differences in genetic food engineering rejection as being based on socio-demographic and informational resp. knowledge based differentials between EU countries and 3) specifying the importance of socio-economic and informational determinants of a...
Tipo: Conference Paper or Presentation
Palavras-chave: Genetic modification; Attitudes towards genetic modification; Biotechnology; Food; Consumer/Household Economics.
Ano: 2002
URL: http://purl.umn.edu/24858

Comparison of Plasmid yield between L.B. and using Molasses as a medium
Provedor de dados: 17
Autores: Nitin Sharma; Purshottum Kaushik.
During the initial studies I have found that the molasses may be a good alternative to costly L.B media where one can manipulate the C/N ratio as per requirement of cell. Molasses is one of the best alternatives as it is cheap and can be easily manipulated. Escherichia coli is a bacterium that is commonly found in the lower intestine of warm-blooded animals. it is possible to produce vaccine by molasses medium. The paper is opening a new face of study.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3897/version/1

Comparison of two surgical approaches for Laparoscopic Ovum Pick Up in Ewes
Provedor de dados: 45
Autores: Mariano,R.S.G.; Uscategu,R.A.R.; Nociti,R.P.; Barros,F.F.P.C.; Santos,V.J.C.; Coutinho,L.N.; Padilha-Nakaghi,L.C.; Silva,P.D.A.; Teixeira,P.P.M.; Vicente,W.R. R.
ABSTRACT The present study had as an aim to evaluate a right lateral access as an alternative method to laparoscopic ovum pick-up (LOPU) in sheep. Twenty-four Santa Ines ewes were randomly assigned in two groups with twelve animals each: RLD - positioned in right lateral decubitus, with 10º head-down tilt; and DD - positioned in dorsal decubitus with 35º head-down tilt. The following parameters were evaluated every 10 minutes during the procedure: total surgical time (ST), visualized follicles (VF), aspirated follicles (AF), recovered oocytes (RO), mean arterial pressure (MAP), heart rate (HR), respiratory rate (fR) and end tidal CO2 pressure (EtCO2). Pre and postoperative arterial hemogasometry parameters (PaO2, PaCO2, pH, CHCO3 and BE) were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovine; Surgery; Biotechnology; Genitourinary.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300848

COMPETITIVE STRATEGIES OF BIOTECHNOLOGY FIRMS: IMPLICATIONS FOR U.S. AGRICULTURE
Provedor de dados: 31
Autores: Begemann,   Brett D.
The agricultural biotechnology industry has evolved from a focus on outstanding science to a more mature phase where firms focus on near-term products and building businesses.  Understanding complex relationships and distribution channels and a global perspective are crucial to commercialization.  Yet, leading-edge technology and early identification of key traits will be critical to developing superior products that ensure competitiveness in the marketplace.   Monsanto is organizing around a life sciences model where seed, crop chemicals, pharmaceuticals, and food ingredient businesses will exploit mutual synergies driven by basic science and discovery.
Tipo: Journal Article
Palavras-chave: Biotechnology; Monsanto; Strategies; Research and Development/Tech Change/Emerging Technologies.
Ano: 1997
URL: http://purl.umn.edu/15540

Complex Systems Analysis of Arrested Neural Cell Differentiation during Development and Analogous Cell Cycling Models in Carcinogenesis
Provedor de dados: 17
Autores: V. I. Prisecaru; I C. Baianu.
A new approach to the modular, complex systems analysis of nonlinear dynamics of arrested neural cell Differentiation--induced cell proliferation during organismic development and the analogous cell cycling network transformations involved in carcinogenesis is proposed. Neural tissue arrested differentiation that induces cell proliferation during perturbed development and Carcinogenesis are complex processes that involve dynamically inter-connected biomolecules in the intercellular, membrane, cytosolic, nuclear and nucleolar compartments. Such &amp;#x27;dynamically inter-connected&amp;#x27; biomolecules form numerous inter-related pathways referred to as &amp;#x27;molecular networks&amp;#x27;. One such family of signaling pathways contains the cell...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Neuroscience; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7101/version/2

Complex Systems Analysis of Arrested Neural Cell Differentiation during Development and Analogous Cell Cycling Models in Carcinogenesis
Provedor de dados: 17
Autores: I C. Baianu; V. I. Prisecaru.
A new approach to the modular, complex systems analysis of nonlinear dynamics of arrested neural cell Differentiation--induced cell proliferation during organismic development and the analogous cell cycling network transformations involved in carcinogenesis is proposed. Neural tissue arrested differentiation that induces cell proliferation during perturbed development and Carcinogenesis are complex processes that involve dynamically inter-connected biomolecules in the intercellular, membrane, cytosolic, nuclear and nucleolar compartments. Such &amp;#x27;dynamically inter-connected&amp;#x27; biomolecules form numerous inter-related pathways referred to as &amp;#x27;molecular networks&amp;#x27;. One such family of signaling pathways contains the cell...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Neuroscience; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7101/version/1

Complex Systems Analysis of Cell Cycling Models in Carcinogenesis
Provedor de dados: 17
Autores: I. C. Baianu.
A new approach to the modular, complex systems analysis of nonlinear dynamics in cell cycling network transformations involved in carcinogenesis is proposed. Carcinogenesis is a complex process that involves dynamically inter-connected biomolecules in the intercellular, membrane, cytosolic, nuclear and nucleolar compartments that form numerous inter-related pathways referred to as networks.&amp;#xd;&amp;#xa;The variable biotopology of such dynamic networks is highly complex, and has a number of interesting properties that can be formally characterized at one level of organization by mathematical structures called &amp;#x27;biogroupoids&amp;#x27;. &amp;#xd;&amp;#xa;One such family of pathways contains the cell cyclins. Cyclins are proteins that link several...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6205/version/1

Comportamento e descrição da linhagem transgênica BRASD 00-12057 RR no estado de Goiás e no Distrito Federal (folder).
Provedor de dados: 14
Autores: FARIAS NETO, A. L. de; MOREIRA, C. T.; SOUZA, P. I. M. de; ABUD, S.; ALMEIDA, L. A. de; TOLEDO, J. F. F.; NUNES JÚNIOR, J.; MONTEIRO, P. M. F. O.; DI STEFANO, J. G.; ASSUNÇÃO, M. S.; ARANTES, N. E.; YORINORI, J. T.; TEIXEIRA, R. N.; DIAS, W. P.; ALMEIDA, A. M. S.; TOLEDO, R. M. C. P.
bitstream/CPAC-2009/27738/1/p2006_66.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Transgênico; Transgenics.; Biotecnologia; Glycine Max; Soja.; Biotechnology; Soybeans.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570203

Computational binding mechanism of Mycobacterium tuberculosis UDP-NAG enolpyruvyl transferase (MurA) with inhibitors fosfomycin, cyclic disulfide analog RWJ-3981, pyrazolopyrimidine analog RWJ-110192, purine analog  RWJ-140998, 5-sulfonoxy-anthranilic aci
Provedor de dados: 17
Autores: C. M. Anuradha; B. Babajan; M. Chaitanaya; C. Rajasekhar; Suresh Kumar Chitta.
Worldwide, tuberculosis (TB) remains the most frequent and important infectious disease causing morbidity and death. One-third of the world&amp;#x27;s population is infected with Mycobacterium tuberculosis (Mtb), the etiologic agent of TB. In this context, TB is in the top three, with malaria and HIV being the leading causes of death from a single infectious agent, and about two million deaths are attributable to TB annually. The bacterial enzyme MurA catalyzes the transfer of enolpyruvate from phosphoenolpyruvate (PEP) to uridine diphospho-N-acetylglucosamine (UNAG), which is the first committed step of bacterial cell wall biosynthesis.  In this work, 3D structural model of Mtb-MurA enzyme has been developed, for the first time, by homology modeling and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3731/version/1

Computational system biology
Provedor de dados: 17
Autores: Ankit Geete.
Systems biology is a multi-disciplinary &amp;#xfb01;eld that deals with mechanisms involved in complex biological processes by considering them as integrated systems of multiple interacting components. The huge amount of data involved in this study necessitates the use of computational tools.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7104/version/1

CONCENTRATION AND TECHNOLOGY IN AGRICULTURAL INPUT INDUSTRIES
Provedor de dados: 31
Autores: King,   John L.
Consolidation in the agricultural biotechnology industry can both enhance and dampen market competition. This report examines the causes and consequences of industry consolidation and its effect on market efficiency. In some cases, concentration realizes economies of scale, which can improve market efficiency by driving down production costs. The protection of intellectual property rights is integral to the agricultural biotechnology marketplace, stimulating research and development, investment, and the development of substitute markets. However, excessively broad intellectual property rights can hinder the market for innovation. Recent data on mergers, acquisitions, and strategic collaborations in the agricultural biotechnology industry, as well as the...
Tipo: Report
Palavras-chave: Industry concentration; Consolidation; Biotechnology; Market efficiency; Market power; Intellectual property rights; Agricultural input industries; Mergers; Acquisitions; Agribusiness; Industrial Organization.
Ano: 2001
URL: http://purl.umn.edu/33631

Concurrent Acquisition of a Single Nucleotide Polymorphism in Diverse Influenza H5N1 Clade 2.2 Sub-clades
Provedor de dados: 17
Autores: Henry L. Niman; Magdi D. Saad; Mona M. Aly; Jeffery  Tjaden; Kenneth C. Earhart; Marshall R. Monteville; Moustafa M. Mansour; Nasr El-Sayed; Ahmed E. Nayel; Ahmed S. Abdelghani; Hala M. Esmat; Emad M. Labib; Ehab A. Ayoub; Abdelattar  Arafa; Gregory A. Raczniak; Mensah Agyen-Frempong; William K. Ampofo; Bruce R. Boynton.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong Kong in 1997. The number of confirmed human cases now exceeds 300, and the associated Case Fatality Rate exceeds 60%. The genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that lack an editing function, thereby resulting in antigenic drift. We report here the concurrent acquisition of the same polymorphism by multiple, genetically distinct, clade 2.2 sub-clades in Egypt, Russia, and Ghana. These changes are not easily explained by the current...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Ecology; Genetics &amp; Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/459/version/3

Concurrent Acquisition of a Single Nucleotide Polymorphism in Diverse Influenza H5N1 Clade 2.2 Sub-clades
Provedor de dados: 17
Autores: Henry L. Niman; Bruce R. Boynton.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong Kong in 1997. The number of confirmed human cases now exceeds 300, and the associated Case Fatality Rate exceeds 60%. The genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that lack an editing function, thereby resulting in antigenic drift. We report here the concurrent acquisition of the same polymorphism by multiple, genetically distinct, clade 2.2 sub-clades in Egypt, Russia, and Ghana. These changes are not easily explained by the current...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/459/version/2

Concurrent Acquisition of a Single Nucleotide Polymorphism in Diverse Influenza H5N1 Clade 2.2 Sub-clades
Provedor de dados: 17
Autores: Henry L. Niman; Bruce R. Boynton.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong Kong in 1997 1,2. The number of confirmed human cases now exceeds 300, and the associated Case Fatality Rate exceeds 60% 3. The genetic diversity of the serotype continues to increase. Four distinct clades or sub-clades have been linked to human cases 4-7. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that lack an editing function, thereby resulting in antigenic drift 8. We report here the concurrent acquisition of the same polymorphism by multiple, genetically distinct, clade 2.2 sub-clades in Egypt, Russia, and Ghana. These changes are not easily explained by the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/459/version/1

Concurrent Acquisition of a Single Nucleotide Polymorphism in Diverse Influenza H5N1 Clade 2.2 Sub-clades
Provedor de dados: 17
Autores: Henry Niman; Mona M. Aly; Abdel-Satar  Arafa; Nasr El-Sayed; Ahmed  E. Nayel; Ahmed  S. Abdelghani; Hala  M. Esmat; Gregory  A. Raczniak; Mensah  Agyen-Frempong; William  K. Ampofo; Bruce R. Boynton.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong Kong in 1997 1,2. The number of confirmed human cases now exceeds 300 and the associated Case Fatality Rate exceeds 60% 3. The genetic diversity of the serotype continues to increase.  Four distinct clades or sub-clades have been linked to human cases 4-7. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that lack an editing function, thereby resulting in antigenic drift 8. We report here the concurrent acquisition of the same polymorphism by multiple, genetically distinct, clade 2.2 sub-clades in Egypt, Russia, Kuwait, and Ghana. These changes are not easily...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/459/version/4

Connecting Seed Lists of Mammalian Proteins Using Steiner Trees
Provedor de dados: 17
Autores: Amelia G. White; Avi Ma&amp;#x27;ayan.
Multivariate experiments and genomics studies applied to mammalian cells often produce lists of genes or proteins altered under treatment/disease vs. control/normal conditions. Such lists can be identified in known protein-protein interaction networks to produce subnetworks that &amp;#x201c;connect&amp;#x201d; the genes or proteins from the lists. Such subnetworks are valuable for biologists since they can suggest regulatory mechanisms that are altered under different conditions. Often such subnetworks are overloaded with links and nodes resulting in connectivity diagrams that are illegible due to edge overlap. In this study, we attempt to address this problem by implementing an approximation to the Steiner Tree problem to connect seed lists of mammalian...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1768/version/1

Consensus virtual screening approaches to predict protein ligands
Provedor de dados: 17
Autores: Andreas Kukol.
In order to exploit the advantages of receptor-based virtual screening, namely time/cost saving and specificity, it is important to rely on algorithms that predict a high number of active ligands at the top ranks of a small molecule database. Towards that goal consensus methods combining the results of several docking algorithms were developed and compared against the individual algorithms. Furthermore, a recently proposed rescoring method based on drug efficiency indices was evaluated. Among AutoDock Vina 1.0, AutoDock 4.2 and GemDock, AutoDock Vina was the best performing single method in predicting high affinity ligands from a database of known ligands and decoys. The rescoring of predicted binding energies with the water/butanol partition coeffcient...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5979/version/1

Consequences of Biotechnology Policy for Competitiveness and Trade of Southern U.S. Agriculture
Provedor de dados: 31
Autores: Jolly,   Curtis M.; Jefferson-Moore,   Kenrett Y.; Traxler,   Greg.
The effect of policy decisions on the competitiveness of genetically modified (GM) crops was examined.  The United States has been an early innovator in the development and use of biotechnology crops and has expanded its export market share of the three major GM crops: soybeans, cotton, and corn.  Cotton, soybeans, and corn are all grown in the southern states, but these states have an apparent comparative advantage only in the production of cotton, which may be strengthened with the adoption of genetically modified cotton.  The influence of biotechnology on the competitiveness of soybeans and corn for the southern states through the introduction of genetically modified organisms (GMOs) is not clear but is probably negligible.
Tipo: Journal Article
Palavras-chave: Biotechnology; Competitiveness; Southern agriculture; Q13; Q17; Q16; Q18.
Ano: 2005
URL: http://purl.umn.edu/43514

Conservation Tillage, Pesticide Use, and Biotech Crops in the U.S.A.
Provedor de dados: 31
Autores: Fernandez-Cornejo,   Jorge; Hallahan,   Charles B.; Nehring,   Richard F.; Wechsler,   Seth James; Grube,   Arthur.
This paper presents the first part of an ongoing project whose objective is to present a long term relationship between conservation tillage, adoption of GE crops and pesticide use for major crops in the United States. In addition, the project aims to provide some innovative tests on causality using a panel data set.  This paper presents preliminary results for soybeans.
Tipo: Conference Paper or Presentation
Palavras-chave: Conservation tillage; Biotechnology; Genetically engineered crops; Soybeans; Herbicides; Environmental Economics and Policy; Farm Management; Production Economics.
Ano: 2010
URL: http://purl.umn.edu/60941

Consortium Building For PEM MFC Using Synthetic Media As Substrate
Provedor de dados: 17
Autores: Muralidharan Ayyappan; Dimpal Parida; Arti Yadav; Ramya Mohandass.
Microbial production of electricity is an important form of bioenergy since Microbial Fuel cells (MFC) offer the possibility of extracting electric current from a wide range of organic wastes and renewable biomass. Factors affecting the MFC operational effectiveness are the MFC design and the bacterial metabolism and electron transfer. The purpose of this study is to identify species which are responsible for electricity generation so as to build a suitable consortium and to investigate the relative efficiencies between the microbial consortiums. Enrichment by repeated transfer of a bacterial consortium harvested from the anode compartment of a MFC with synthetic media as a substrate increased the output from an initial level of 34 mA to a maximal level of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6632/version/1

Consumer Acceptance of Genetically Modified Food Products in the Developing World
Provedor de dados: 31
Autores: Curtis,   Kynda R.; Wahl,   Thomas I.; McCluskey,   Jill J.
World-wide consumer response toward food products made from genetically modified ingredients has been largely negative. However, the majority of the previous studies on consumer attitudes towards genetically modified food products were conducted in developed countries in Europe as well as Japan. The small number of studies conducted in developing countries obtained different results from the developed world. This paper considers the motivations for consumer attitudes towards genetically modified foods in developing countries. We conclude that the generally positive perception towards genetically modified foods in developing countries could be the result of a cost-benefit analysis consistent with expected utility theory. Developing countries have more...
Tipo: Conference Paper or Presentation
Palavras-chave: Developing countries; Risk; Biotechnology; Genetically modified foods; Crop Production/Industries; Risk and Uncertainty.
Ano: 2003
URL: http://purl.umn.edu/57858

CONSUMER RISK PERCEPTION PROFILES FOR THE FOOD-RELATED BIOTECHNOLOGY,  RECOMBINANT BOVINE GROWTH HORMONE (rbGH)
Provedor de dados: 31
Autores: Grobe,   Deana; Douthitt,   Robin; Zepeda,   Lydia.
Tipo: Conference Paper or Presentation
Palavras-chave: Consumer risk perception; Biotechnology; Bovine growth hormone; Food Consumption/Nutrition/Food Safety.
Ano: 1997
URL: http://purl.umn.edu/25953

CONSUMER VALUATION OF GENETICALLY MODIFIED FOODS AND THE EFFECT OF INFORMATION BIAS
Provedor de dados: 31
Autores: Vanwechel,   Tamara; Wachenheim,   Cheryl J.; Schuck,   Eric C.; Lambert,   David K.
Bid prices were elicited for standard-label cookies, muffins, and potato chips and those identified as not including genetically modified (GM) ingredients using an experimental auction.  Including a statement that the product did not include GM ingredients increased bids over those offered for standard-label products.  Providing negative-biased information about the impact of GM crops on the environment increased the risk participants associated with GM foods, and positive-biased information decreased perceived risk.  Overall, providing impact information, whether positive- or negative-biased, increased bids for products presumed GM.  The influence of information bias on bids varied among selected participant groups, supporting the presence of uniquely...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Environment; Experimental auction; Genetically modified; Information; Willingness-to-pay; Food Consumption/Nutrition/Food Safety; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/23620

Consumers' Willingness to Pay for Genetically Modified Foods in Kenya
Provedor de dados: 31
Autores: Kimenju,   Simon Chege; De Groote,   Hugo.
A survey of 600 consumers was conducted in Nairobi to determine attitudes and willingness to pay (WTP) for GM maize meal. WTP was estimated using the double-bounded logit model. Overall, 38% are aware of GM crops. Most consumers believe in the technology's positive impacts, but are concerned about environmental and health risks. Majority (68%) would buy GM maize meal at the price of their favourite maize meal brand. The mean WTP for GM maize meal is KShs 58 for a 2kg packet, a premium of 13.7 % over mean average price of favourite brands. WTP is influenced more by subjective than socioeconomic characteristics.
Tipo: Conference Paper or Presentation
Palavras-chave: GMO; Biotechnology; Consumer; Africa; Maize; Consumer/Household Economics; D12; C25; Q16.
Ano: 2005
URL: http://purl.umn.edu/24504

Control in technological systems and physical intelligence: an emerging theory
Provedor de dados: 17
Autores: Bradly Alicea.
The transduction and processing of physical information is becoming important in a range of research fields, from the design of materials and virtual environments to the dynamics of cellular microenvironments. Previous approaches such as morphological computation/soft robotics, neuromechanics, and embodiment have provided valuable insight. This work approaches haptic, proprioception, and physical sensing as all part of the same subject. In this presentation, three design criteria for applying physical intelligence to engineering applications will be presented. These criteria have several properties in common, which inspires two types of end-effector model: stochastic (based on a spring) and deterministic (based on a piezomechanical array). The generalized...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Neuroscience.
Ano: 2011
URL: http://precedings.nature.com/documents/5910/version/1

Controlling sap-sucking insect pests with recombinant endophytes expressing plant lectin
Provedor de dados: 17
Autores: Xiuyun Zhao; Gaofu Qi; Xianfang Zhang; Nan Lan; Xinrong Ma.
We developed a novel pest management strategy, which uses endophytes to express anti-pest plant lectins. Fungal endophyte of Chaetomium globosum YY-11 with anti-fungi activities was isolated from rape seedlings, and bacterial endophytes of SJ-10 (Enterobacter sp.) and WB (Bacillus subtilis) were isolated from rice seedlings. Pinellia ternate agglutinin gene was cloned into SJ-10 and WB for expression by a shuttle vector, and YY-11 was mediated by Agrobacterium tumefaciens. Positive transformants were evaluated using PCR and Western blot assay. Recombinant endophytes colonized most of crops, and resistance of rice seedlings, which were inoculated with the recombinant endophytic bacteria, to white backed planthoppers was dramatically enhanced by decreasing...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4862/version/1

Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
Provedor de dados: 96
Autores: Machado,Gilmara Junqueira; Murgas,Luis David Solis; Palhares,Priscila Cotta; Assis,Isadora de Lima; Castro,Tássia Flávia Dias.
ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; Biotechnology; Cryopreservation; Eggs; Fish; Larvae.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155

Copper induced peroxidase, CCPP form Caribbean copper plant
Provedor de dados: 17
Autores: Reetesh Kumar.
Plant peroxidase exhibit broad substrate specificity and is active in a wide range of pH and temperature, and in the presence of organic compounds as well as other additives. The peroxidase is known to be involved in many physiological and regulatory processes in plants. This ppt presents an introduction on plant peroxidase and their applications. A purification protocol has been developed and the purified protein, named as CCPP is biochemically and physiologically characterized. The physiochemical properties such as molecular weight, isoelectric point have been experimentally determined. The enzymatic efficiency has been evaluated through temperature optimum, pH optimum, Km and other enzymatic properties along with spectroscopic parameters like circular...
Tipo: Presentation
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6410/version/1

Cost effective mass multiplication of Gymnema sylvestre in hydroponic system
Provedor de dados: 17
Autores: Raju Karthic; Sundaram Seshadri.
Present experiment was designed with an objective to develop a cost efficient mass multiplication method for Gymnema sylvestre using hydroponic system. A plastic tub, with polyethylene cover, containing 1/10 strength of MS salts supplemented with Indole butyric acid (IBA) at different concentrations (0.5, 1.0, 2.5 mg/L) was studied. Medium containing 0.5mg/L of IBA produced highest rooting (66%) with 96 % survival. This protocol will serve as an alternative to the existing in vitro and clonal multiplication protocols.
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3770/version/1

COSTS AND RISKS OF TESTING AND SEGREGATING GM WHEAT
Provedor de dados: 31
Autores: Wilson,   William W.; Dahl,   Bruce L.
Development of genetically modified crops is challenging the functions of the grain marketing system with many participants arguing for Identity Preservation systems prior to release of GM varieties.  In this study, a stochastic optimization model was developed to determine optimal testing strategies.  The model chooses the optimal testing strategy that maximizes utility (minimizes disutility) of additional system costs due to testing and rejection and allows estimation of the risk premium required for sellers to undertake a dual marketing system with GM/Non-GM segregations over the current Non-GM system.  Elements of costs (testing costs, rejection costs, and risk premium) were estimated for a base model representing a grain export chain.  The model...
Tipo: Working or Discussion Paper
Palavras-chave: Genetically modified organisms; Biotechnology; Wheat; Risk; Segregation; Identity preservation; Crop Production/Industries.
Ano: 2002
URL: http://purl.umn.edu/23480

COSTS AND RISKS OF TESTING AND SEGREGATING GM WHEAT - SUMMARY
Provedor de dados: 31
Autores: Wilson,   William W.; Dahl,   Bruce L.
Development of genetically modified crops is challenging the functions of the grain marketing system with many participants arguing for Identity Preservation (IP) systems prior to release of GM varieties.  In this study, a stochastic optimization model was developed to determine optimal testing strategies.  The model chooses the optimal testing strategy that maximizes utility (minimizes disutility) of additional system costs due to testing and rejection and allows estimation of the risk premium required for sellers to undertake a dual marketing system with GM/Non-GM segregations over the current Non-GM system.  Elements of costs (testing costs, rejection costs, and risk premium) were estimated for a base model representing a grain export chain.  The model...
Tipo: Working or Discussion Paper
Palavras-chave: Genetically modified organisms; Biotechnology; Wheat; Risk; Segregation; Identity preservation; Crop Production/Industries.
Ano: 2002
URL: http://purl.umn.edu/23502

Co-transformation of Oil Palm Using Agrobacterium-Mediated Transformation
Provedor de dados: 17
Autores: Vu Le.
Agrobacterium-mediated transformation is being used as an alternative method for improving the conventional breeding. In this study, immature embryos (IEs) of oil palm are chosen as explants for transformation. &amp;#x3b2;-glucuronidase (gus) and green fluorescence protein (GFP) were harbored by the two plasmids pCAMBIA 1304 and pCAMBIA 1302 respectively. The results were obtained at least four days after transformation. The IEs placing in the N6O medium for 7 days were then co-cultivated with A.tumefaciens suspension within 30 mins and then incubated in the dark for 1 day. In single transformation, GUS assay revealed that about 64% transient transformation frequency was obtained for IEs two weeks after co-cultivation, GFP activity was recorded as 41.67%...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/5441/version/1

Cottonseed cake: Detoxification process of cottonseed cake.
Provedor de dados: 14
Autores: SIQUEIRA, F. G. de.
Technology for obtaining cellulose nanofibers from agro-industriaL residues or pure cellulose sources by pretreatment and hydrolysis.
Tipo: Folders
Palavras-chave: Cottonseed cake; Detoxification (processing); Biotechnology.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1133078

Counting absolute number of molecules using unique molecular identifiers
Provedor de dados: 17
Autores: Teemu Kivioja; Anna V&amp;#xe4;h&amp;#xe4;rautio; Kasper Karlsson; Martin Bonke; Sten Linnarsson; Jussi Taipale.
Advances in molecular biology have made it easy to identify different DNA or RNA species and to copy them. Identification of nucleic acid species can be accomplished by reading the DNA sequence; currently millions of molecules can be sequenced in a single day using massively parallel sequencing. Efficient copying of DNA-molecules of arbitrary sequence was made possible by molecular cloning, and the polymerase chain reaction. Differences in the relative abundance of a large number of different sequences between two or more samples can in turn be measured using microarray hybridization and/or tag sequencing. However, determining the relative abundance of two different species and/or the absolute number of molecules present in a single sample has proven much...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5903/version/1

CREATING, PROTECTING, AND USING CROP BIOTECHNOLOGIES WORLDWIDE IN AN ERA OF INTELLECTUAL PROPERTY
Provedor de dados: 31
Autores: Pardey,   Philip G.; Koo,   Bonwoo; Nottenburg,   Carol.
Proponents tout the positive incentive-to-innovate effects of intellectual property rights (IPRs), while others maintain that the expanding subject matter and geographical extent of IPRs are stifling crop research, especially research and development (R&amp;D) dealing with developing-country crop concerns.  Much of this debate relies on anecdotes and misleading or incomplete evidence on the extent and nature of the IPRs pertaining to crop technologies, including the jurisdictional extent of the property rights and their practice.  In this paper we review the evidence on the scope of agricultural R&amp;D worldwide, provide new data on the structure of crop-related IPRs, and summarize trends on the uptake of proprietary bioengineered crops.
Tipo: Working or Discussion Paper
Palavras-chave: Plant patents; Utility patents; Plant breeders' rights; Crop varieties; Public and private agricultural R&amp;D; Biotechnology; Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/13600

Crisis as a catalyst: the role of Schumpeterian innovation in the Lithuanian economy
Provedor de dados: 31
Autores: Giedraitis,   Vincentas Rolandas; Rasteniene,   Ausra.
What circumstances allow businesses to flourish in a stagnant world economy? We ask that question in our discussion of the uniquely favorable circumstances of the biotechnology sector in Lithuania. The purpose of this paper is to analyze Lithuania’s ability to expand its economy during a time of crisis, focusing on its unique ability to innovate in such sectors as biotechnology.
Tipo: Journal Article
Palavras-chave: Schumpeter; Innovation; Kondratiev wave; Biotechnology; Business cluster theory; Financial Economics; International Development; Research and Development/Tech Change/Emerging Technologies; O31; O32.
Ano: 2009
URL: http://purl.umn.edu/94527

Critical Concentration Gradient Model of Bacterial Cell Division
Provedor de dados: 17
Autores: Aijaz A. Rashid; Perminder P. Sing.
Critical concentration gradient model is helpful in understanding the mechanism of Z-ring formation. The distribution of positive and negative regulators of Z-ring formation in cell is not random but well programmed. The positive and negative regulators in cell lead to a critical concentration gradient from mid cell to the poles. The critical concentration of FtsZ required for Z-ring assembly at the centre of cell is less than that at the poles. So FtsZ starts polymerizing at cell centre and afterwards by cooperative association of more and more monomers leads to the Z-ring formation. Simultaneously during Z-ring formation the recruitment of other accessory proteins takes place, leading to the formation of a stable divisome complex.
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4659/version/1

Crop Research Incentives in a Privatized Industry: A Stochastic Approach
Provedor de dados: 31
Autores: Malla,   Stavroula; Gray,   Richard S.
We model today's privatized crop research industry as a small number of firms, developing and selling differentiated products to heterogeneous producers. Crop variety research is modeled as a search process, which allows us to differentiate between applied and basic research and recognize research as a stochastic process. We use the framework to develop a number of propositions regarding private research incentives, the spillovers of knowledge, and the impact of public policy. The results suggest an underinvestment in research even when property rights have been established.
Tipo: Conference Paper or Presentation
Palavras-chave: Search process; Stochastic process; Biotechnology; IPRs; Applied R&amp;D; Basic R&amp;D; Imperfect competition; Differentiated products; Heterogeneous producers.; Agribusiness.
Ano: 2002
URL: http://purl.umn.edu/24936

Crosstalk and the spectrum of biological global broadcasts: Toward generalization of the Baars consciousness model across physiological subsystems
Provedor de dados: 17
Autores: Rodrick Wallace.
Once cognitive biological phenomena are recognized as necessarily having &amp;#x27;dual&amp;#x27; information sources, it is easy to show that the information theory chain rule implies isolating coresident information sources from crosstalk requires more metabolic free energy than permitting correlation. This provides conditions for an evolutionary exaptation leading to dynamic global broadcasts of interacting cognitive biological processes analogous to, but slower than, consciousness, itself included within the paradigm. The argument is closely analogous to the well-studied exaptation of noise to trigger stochastic resonance amplification in physiological systems.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Immunology; Molecular Cell Biology; Neuroscience; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6898/version/1

Cryopreservation of a whole liver
Provedor de dados: 17
Autores: Zohar Gavish; Amir Arav; Menahem Ben-Haim.
Preservation of vascularized organs such as the liver is limited to 24hrs. before destructive processes disqualify it for transplantation. This narrow time window prevents surgeons from performing optimal pathogen screening and matching tests which often lead to re-transplantation. Numerous problems are associated with viably freezing and thawing a whole liver: complicated geometry, poor heat transfer, release of latent heat and the difficulty of generating a uniform cooling rate. Our past success led us to apply our novel freezing technique to a larger solid organ, the liver. Whole livers were frozen/thawed using a directional solidification apparatuses; viability was tested by means of integrity and functionality in vitro and in auxiliary liver...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1352/version/1

Cryopreservation of cell/hydrogel constructs based on a new cell-assembling technique
Provedor de dados: 17
Autores: Xiaohong  Wang; Huirong  Xu; Yongnian  Yan; Renji  Zhang.
Organ manufacturing products hold the promise to be used widely in the future for complex organ failings. The cryopreservation of the product is a very important step in the commercialization activities. In this article, a new cell cryopreservation technique, whereby, cryoprotectants were directly incorporated into the cell/hydrogel constructs, prototyped according to the predesigned structure and then subjected to a special freezing/thawing process. The rheological and hydration properties of the cryopreservation systems indicated that the hydratabilities of the gelatin/alginate hydrogels were greatly increased while the eutectic temperatures were greatly decreased by the addition of glycerol. Dextran-40 was found to be effective to improve the cell...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3722/version/1

Cultivares de soja transgênicas da Embrapa indicadas para o sistema de produção da região do cerrado (folder).
Provedor de dados: 14
Autores: SOUZA, P. I. M. de; MOREIRA, C. T.; FARIAS NETO, A. L.; ABUD, S.; NUNES JÚNIOR, J.; MONTEIRO, P. M. F. O.; DIAS, W. P.; ALMEIDA, L. A.; TOLEDO, J. F. F.; ASSUNÇÃO, M. S.; DI STEFANO, J. G.; ARANTES, N. E.; TOLEDO, R. M. C. P.
bitstream/CPAC-2009/27685/1/p2006_24.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Transgênico; Transgenics.; Biotecnologia; Glycine Max; Soja.; Biotechnology; Soybeans.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570161

Current Contribution of Four Biotechnologies to New Zealand's Primary Sector
Provedor de dados: 31
Autores: Kaye-Blake,   William; Saunders,   Caroline M.; Emanuelsson,   Martin.
Over the last several decades, changes to production technologies have significantly increased output from the primary sector. The contribution of improved genetics has been variously estimated, and the impacts of genetic engineering have received much attention. The present research estimated the economic impact of four modern biotechnologies (other than genetic engineering) on the primary sector. Information from interviews with key informants was incorporated into a cost-benefit analysis to estimate these impacts. The net contribution was estimated at $266 million per year, with impacts spread unevenly through the sector and across biotechnologies.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Cost-benefit analysis; Marker assisted selection; New Zealand; Primary production; Research and Development/Tech Change/Emerging Technologies; O30; Q16.
Ano: 2006
URL: http://purl.umn.edu/25411

Date and their processing byproducts as substrates for bioactive compounds production
Provedor de dados: 52
Autores: Tang,Zhen-Xing; Shi,Lu-E; Aleid,Salah Mohammed.
Date is the most popular fruit in middle-east countries. However, the date production process is accompanied by a substantial increase of loss during picking, storage, commercialization and conditioning process. Date and their byproducts have many essential elements for the growth of microorganisms. Thus, they can be converted into value-added compounds through biotechnology. In this paper, date and their processing byproducts used as substrates for producing value-added products such as organic acids, exopolysaccharide, antibiotics, date flavored probiotic fermented dairy, bakery yeast, etc, were reviewed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Date; Byproduct; Biotechnology; Fermentation; Review.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500706

Density alteration in non-physiological cells
Provedor de dados: 17
Autores: Yu Liu; Yuan-Chang Zhu; Bin-Bin Wu; Xiao-Hua Wu; Fei-Fei Lan; Jian-Ge Wei; Ming Wang; Hai-Cheng Li; Lin-Na Li; Ju-Xiang Li; Jia Fei.
In the present study an important phenomenon of cells was discovered: the change of intracellular density in cell&amp;#x27;s response to drug and environmental factors. For convenience, this phenomenon is named as &amp;#x22;density alteration in non-physiological cells&amp;#x22; ( DANCE). DANCE was determined by discontinuous sucrose gradient centrifugation (DSGC), in which cells were separated into several bands. The number and position of the bands in DSGC varied with the change of cell culture conditions, drugs, and physical process, indicating that cell&amp;#x27;s response to these factors was associated with alteration of intracellular density. Our results showed that the bands of cells were molecularly different from each other, such as the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6541/version/1

Description of a method for localizing swarming mosquitoes and other insects in 3D space with visualizations
Provedor de dados: 17
Autores: Nicholas C. Manoukis.
Male mosquitoes swarm to find mates, but the characteristics of these swarms have not yet been quantitatively analyzed in the field. This paper describes a simple method used recently to localize individual Anopheles gambiae in a swarm via stereoscopic image analysis of video footage swarms recorded in Doneguebougou, Mali. The methods described here represent a streamlining of simple triangulation for insect localization in the hope that other researchers will be able to apply the method for studying fundamental questions about flying insects or other taxa.&amp;#xd;&amp;#xa;
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology.
Ano: 2008
URL: http://precedings.nature.com/documents/2599/version/1

Desempenho agronômico de isso-híbridos de milho safrinha em Dourados, MS.
Provedor de dados: 14
Autores: SILVA, J. A. dos S.; MATHEUS VICENTINI CORREIA; SILVA NETO, A. A. da; CECCON, G.
bitstream/item/189954/1/ORAL14.pdf
Tipo: Outras publicações técnicas (INFOTECA-E)
Palavras-chave: Milho; Biotecnologia; Produtividade; Grão; Zea Mays; Biotechnology; Corn.
Ano: 2018
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1103274

Design and construction of a new Drosophila species, D.synthetica, by synthetic regulatory evolution
Provedor de dados: 17
Autores: Eduardo Moreno.
Here, I merge the principles of synthetic biology^1,2^ and regulatory evolution^3-11^ to create a new species^12-15^ with a minimal set of known elements. Using preexisting transgenes and recessive mutations of Drosophila melanogaster, a transgenic population arises with small eyes and a different venation pattern that fulfills the criteria of a new species according to Mayr&amp;#x27;s &amp;#x22;Biological Species Concept&amp;#x22;^7,10^. The genetic circuit entails the loss of a non-essential transcription factor and the introduction of cryptic enhancers. Subsequent activation of those enhancers causes hybrid lethality. The transition from &amp;#x22;transgenic organisms&amp;#x22; towards &amp;#x22;synthetic species&amp;#x22;, such as Drosophila...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6511/version/1

Design of a dynamic model of genes with multiple autonomous regulatory modules by evolution in silico
Provedor de dados: 17
Autores: Alexander V. Spirov; David M. Holloway.
A new approach to design a dynamic model of genes with multiple autonomous regulatory modules by evolution in silico is proposed. The approach is based on Genetic Algorithms, with new crossover operators especially designed for these purposes. The approach exploits the subbasin-portal architecture of the fitness functions suitable for this kind of evolutionary modeling. The effectiveness of the approach is demonstrated on a series of benchmark tests.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics &amp; Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3913/version/1

Design of a dynamic model of genes with multiple autonomous regulatory modules by evolution in silico
Provedor de dados: 17
Autores: Alexander V. Spirov; David M. Holloway.
A new approach to design a dynamic model of genes with multiple autonomous regulatory modules by evolution in silico is proposed. The approach is based on Genetic Algorithms, with new crossover operators especially designed for these purposes. The approach exploits the subbasin-portal architecture of the fitness functions suitable for this kind of evolutionary modeling. The effectiveness of the approach is demonstrated on a series of benchmark tests.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Genetics &amp; Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/3913/version/2

Design of selective peptide antibiotics by using the sequence moment concept
Provedor de dados: 17
Autores: Davor Juretic; Nada Ilic; Damir Vukicevic; Nikolinka Antcheva; Alessandro Tossi.
New antibiotics against multidrug-resistant bacteria are urgently needed, but rapid acquisition of resistance limits their usefulness. Endogenous antimicrobial peptides (AMPs) with moderate selectivity, but multimodal mechanism of action, have remained effective against bacteria for millions of years. Their therapeutic application, however, requires optimizing the balance between antibacterial activity and selectivity, so that rational design methods for increasing selectivity are highly desirable. We have created training (n=36) and testing (n=37) sets from frog-derived AMPs with determined therapeutic index (TI). The &amp;#x27;sequence moments&amp;#x27; concept then enabled us to find a one-parameter linear model resulting in a good correlation between...
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3068/version/1

Design of selective peptide antibiotics by using the sequence moment concept
Provedor de dados: 17
Autores: Davor Juretic; Nada Ilic; Damir Vukicevic; Nikolinka Antcheva; Alessandro Tossi.
Withdrawn&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;Incomplete data
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3068/version/2

Designing L-Systems for making three and six open reading frames from the leading strand of a single DNA molecule
Provedor de dados: 17
Autores: Arunava Goswami; Rajneesh Singh; Pabitra Choudhury; Sk. Sarif Hassan Hassan.
Current molecular biology dogma (except alternative splicing) suggests that leading strand of DNA produces mRNA and usually the leading strand gives one ORF (ORF) among the available six ORFs. We attempted to break this rule where three and six ORFs from the leading strand will be formed by designing L-Systems, originally proposed to study symmetry of biological world. We thought this would revolutionize the world of synthetic biology. While doing so, we found L-System containing A&amp;#x2192;T, T&amp;#x2192;G, C&amp;#x2192;A, and G&amp;#x2192;C, i.e., single nucleotide production rule will stop growing after one nucleotide. L-System containing either (a) A&amp;#x2192;TG, T&amp;#x2192;GC, C&amp;#x2192;GA, G&amp;#x2192;TC or (b) A&amp;#x2192;CTG,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4844/version/1

Detection of genetic diversity among Indian strains of _Xanthomonas campestris_ pv. _mangiferaeindicae_ using PCR-RAPD
Provedor de dados: 17
Autores: Ram Kishun; Vijai Kumar Gupta.
The randomly amplified polymorphic DNA (RAPD) technique was used to investigate the genetic diversity in 6 strains of _Xanthomonas campestris_ pv. _mangiferaeindicae_ (_Xcmi_), the causal pathogen of mango bacterial canker disease (MBCD). The RAPD analysis was also intended to identify molecular markers, specific to the species to develop PCR-based markers for detection of _Xcmi_ in mango field and planting materials. Twenty RAPD primers (CP 1-CP 20) were evaluated to establish molecular characters and genetic variability in the genome of _Xcmi_. Among these, only 4 were found efficient for development of reproducible banding pattern. It has been observed that the largest and smallest amplified RAPD products were of 2.036 and 0.201 kbp. A total of 136...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/2403/version/1

Detection of Metabolites by Proton Ex Vivo NMR, in Vivo MR Spectroscopy Peaks and Tissue Content Analysis: Biochemical-Magnetic Resonance Correlation: Preliminary Results
Provedor de dados: 17
Autores: Rakesh Sharma; Madan Rehani; Arun Agrawala.
*Aim*: Metabolite concentrations by in vivo magnetic resonance spectroscopy and ex vivo NMR spectroscopy were compared with excised normal human tissue relaxation times and tissue homogenate contents.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;*Hypothesis*: Biochemical analysis combined with NMR and MR spectroscopy defines better tissue analysis.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;*Materials and Methods*: Metabolites were measured using peak area, amplitude and molecular weights of metabolites in the reference solutions. In normal brain and heart autopsy, muscle and liver biopsy tissue ex vivo NMR peaks and spin-lattice (T1) and spin-spin (T2) relaxation times, were compared with diseased tissue NMR data in meningioma brain, myocardial infarct heart,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/4121/version/1

Detection of substrate binding motifs for morphine biosynthetic pathway intermediates in novel wound inducible (R,S)-reticuline 7-O-methyltransferase of Papaver somniferum
Provedor de dados: 17
Autores: Sonal Mishra; Abha Meena; Sema Singh; Dharmendra Kumar Yadav; Feroz  Khan; Rakesh K. Shukla.
The benzylisoquinoline alkaloids (BIA) comprise a large and diverse group of nitrogen-containing secondary metabolites with about 2500 compounds identified in plants. BIA biosynthesis begins with the condensation of the tyrosine derived precursors dopamine and p-hydroxyphenylacetaldehyde to (S)-norcoclaurine. Subsequent regiospecific O- and N-methylations and aromatic ring hydroxylation lead to (S)-reticuline, which is the central intermediate for almost all BIAs. For morphinan alkaloid biosynthesis, (S)-reticuline undergoes an inversion of stereochemistry to (R)-reticuline, followed by C-C phenol coupling catalyzed by a unique cytochrome P450-dependent monooxygenase to yield salutaridine. The cDNA sequence of enzymes leading to (S)-reticuline, as well as...
Tipo: Poster
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics; Plant Biology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5790/version/1

Detection strategies for non-authorized GMO products on the European market
Provedor de dados: 17
Autores: Patrick G&amp;#xfc;rtler; Esther  Meissner; Ulrich  Busch.
Import and planting of biotech crops is strictly regulated in Europe. In order to observe these strict regulations, an efficient surveillance of the European market is required. In Europe, data on authorised biotech crops from foreign countries are on hand, however, only scarce information on non-authorised biotech crops is available. Experience of the last years has shown that non-authorized biotech crops (e.g. biotech-papaya, Bt10-maize, LL601-rice and Bt63-rice) were detected on the European market. The Bavarian Health and Food Safety Authority is responsible for the detection of illegal imports and for labelling control of Food and Feed in Bavaria (Germany). The quantitative real-time PCR is the method of choice for the detection of genetic...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4444/version/1

Detection systems cross reacting with peptides - Analysis at the amino acid level
Provedor de dados: 17
Autores: Carsten C. Mahrenholz; Victor  Tapia; Rolf Stigler; Rudolf Volkmer.
There are several methods commonly used to measure protein-protein interactions and binding affinities. Quite contrary to most of these methods, protein- and peptide arrays on cellulose membranes or glass slides are suitable for high-throughput measurement, as they provide a higher density of probes and a multitude of peptide-protein interactions can be measured in parallel [1]. The most important application of the SPOT synthesis technique is to simultaneously detect a high number of peptides that have a strong binding affinity to defined targets. The validity of the results, however, depends on the ability of the detection system to indicate binding events whilst not interfering with the experiment itself through cross reaction. We tested three common...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4448/version/1

Determination of Isoflavone Contents for Selected Soybean Lines and Soy Foods by  Fourier Transform Near Infrared Reflectance Spectroscopy
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Randall L. Nelson.
Soybean isoflavones are of considerable interest in relation to their possible health effects in human diets. Rapid and economical determination of soybean isoflavone contents is essential for breeding and selection of soybean seeds with optimal isoflavone levels for food applications. Fourier Transform Near Infrared Spectroscopy (FT-NIR) calibrations were developed for rapid and reliable analysis of soybean isoflavone content. Our isoflavone calibrations are characterized by low standard errors (&amp;#x3c;0.02%) and high degrees of correlation (&amp;#x3e;99%).
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Immunology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6194/version/1

Determination of Soybean Oil, Protein and Amino Acid Residues in Soybean Seeds by High Resolution Nuclear Magnetic Resonance (NMRS) and Near Infrared (NIRS)
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng  You; Doina Costescu; P R. Lozano; Valentin Prisecaru; Randall   L. Nelson.
A detailed account is presented of our high resolution nuclear magnetic resonance (HR-NMR) and near infrared (NIR) calibration models, methodologies and validation procedures, together with a large number of composition analyses for soybean seeds. NIR calibrations were developed based on both HR-NMR and analytical chemistry reference data for oil and twelve amino acid residues in mature soybeans and soybean embryos. This is our first report of HR-NMR determinations of amino acid profiles of proteins from whole soybean seeds, without protein extraction from the seed.  It was found that the best results for both oil and protein calibrations were obtained with a Partial Least Squares Regression (PLS-1) analysis of our extensive NIR spectral data, acquired...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Ecology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics; Plant Biology; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7053/version/1

Deterministic Separation of Cancer Cells from Blood at 10 mL/min
Provedor de dados: 17
Autores: Kevin Loutherback; Joseph D&amp;#x27;Silva; Liyu Liu; Amy Wu; Robert Austin; James Sturm.
Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate, propagate and characterize viable CTCs and clusters. Here, we present a microfluidic device for mL/min flow rate, continuous-flow capture of viable CTCs from blood using deterministic lateral displacement arrays. We show here that a deterministic bump array can be designed such that it will isolate with efficiency greater than 85% CTCs over a large range in sizes from...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6861/version/1

Development and comparison of ECC-rtPCR with in vivo method to determine the viability and infectivity of Giardial cysts
Provedor de dados: 17
Autores: M. Khalid Ijaz; Absar Alum; Basel  Sbai; Hamas  Asaad; Joseph Rubino.
Globally, Giardia is one of the major cause of diarrheal illnesses and rapid diagnostic methods differentiating infectious cysts are critical for developing intervention strategies through contaminated surfaces, food and water. This is currently hampered by lack of an in vitro model.  We evaluated mRNA expression in trophozoites and their attachment to a CaCo2 (C2bb) cell monolayer and changes in trans-cellular resistance as an indicator of Giardia viability and infectivity.  Heat shock mRNA in Giardia cysts and variant specific protein (VSP) mRNA in trophozoites were quantified by RT-PCR. When compared with neonatal mice infectivity, the attachment of trophozoites to cell monolayers, expression of VSP, and changes in the trans-cellular resistance, the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology; Earth &amp; Environment.
Ano: 2011
URL: http://precedings.nature.com/documents/5501/version/1

Development of a Decision Support System (DSS) for an integrated test system towards the effective and competitive risk assessment on transgenic plants
Provedor de dados: 17
Autores: Christine  H&amp;#xf6;flich; Andreas  M&amp;#xfc;ller; J&amp;#xf6;rg  Schmidtke; Kerstin  Schmidt; Inge  Broer.
Transgenic plants need specific approaches to analyse their potential impact on environment and consumer. The procedures used to date are often too extensive and time-consuming and associated with high costs. The BioOK network was established to develop an effective and competitive risk assessment on transgenic plants based on interdisciplinary research. New and effective methods for the risk assessment were established which focus on transgene-specific effects measured in in vitro systems in the laboratory or greenhouse whenever possible. Nevertheless event-specific field experiments have to be carried out in addition to implement environmental effects e.g. on non-targets or the variability of transgene expression.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4438/version/2

Development of a Decision Support System (DSS) for an integrated test system towards the effective and competitive risk assessment on transgenic plants
Provedor de dados: 17
Autores: Christine  H&amp;#xf6;flich; Andreas  M&amp;#xfc;ller; J&amp;#xf6;rg  Schmidtke; Kerstin  Schmidt; Inge  Broer.
Transgenic plants need specific approaches to analyse their potential impact on environment and consumer. The procedures used to date are often too extensive and time-consuming and associated with high costs. The BioOK network was established to develop an effective and competitive risk assessment on transgenic plants based on interdisciplinary research. New and effective methods for the risk assessment were established which focus on transgene-specific effects measured in in vitro systems in the laboratory or greenhouse whenever possible. Nevertheless event-specific field experiments have to be carried out in addition to implement environmental effects e.g. on non-targets or the variability of transgene expression.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4438/version/1

Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10 degrees Centigrade
Provedor de dados: 17
Autores: Uttam Datta; M Chandrasekar; Manik Lal Hembram; Raju Dasgupta.
Electrolyte free mediums prepared with soybean lecithin-glycerol,Coenzyme Q10&amp;#x2013;glycerol and soybean lecithin-Coenzyme Q10-glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5 degree centigrade, wrapped with aluminium foils along with testis and freezed at -10 degree centigrade.Spermatozoan characters were evaluated  7 and 21 days after thawing at 38.5 degree centigrade in a water bath for 5 min. Spermatozoan characters were diminished gradually and significantly (P&amp;#x3c;0.001, P&amp;#x3c;0.05) between the mediums and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, livability, hypo-osmotic swelling...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3377/version/1

Development of a sampling regime for research with environmental relevance based on variability of transgene expression in plants
Provedor de dados: 17
Autores: Carla  Struzyna-Schulze; Heike  Mikschofsky; Wenke  M&amp;#xf6;nkemeyer; J&amp;#xf6;rg  Schmidtke; Kerstin  Schmidt; Inge  Broer.
Transgenic plants often produce proteins that are unknown in conventional crops. Therefore, it requires a fundamental analysis before approval of a new genetically modified plant is given. Since the EFSA-guidelines for risk assessment on transgenic plants provide only recommendations but no detailed instructions, we wanted to develop a valid test scheme that defines sample size and sampling organ to enable a statically valid and efficient analysis of transgene expression and its variability. The scheme will be based on data obtained for the variability in expression i. of different recombinant proteins in one variety; ii. of different integration sites of one transgene in one variety; iii. of the same recombinant protein between different varieties and...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4443/version/1

Development of globular embryos from the hybridization between 'Pêra Rio' sweet orange and 'Poncã' mandarin
Provedor de dados: 94
Autores: Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene Alves de.
This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo culture; Tissue culture; Biotechnology; Citrus breeding.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031

Development of Java based graphical user interface for Diagnosis of Hepatitis UsingI Mixture of Expert
Provedor de dados: 17
Autores: M Shankaracharya; Suchitra Kumari; Ambarish S. Vidyarthi.
Hepatitis is deadly, and the fifth leading cause of death after heart disease, stroke, chest disease and cancer. Worldwide, 1.5 million deaths per year have been estimated. Detection of hepatitis is a big problem for general practitioners. An expert doctor commonly makes decisions by evaluating the current test results of a patient or by comparing the patient with others with the same condition with reference to the previous decisions. Many machine learning and data mining techniques have been designed for the automatic diagnosis of hepatitis. However, no one tool is available to the general population for the diagnosis of Hepatitis. Hence, a graphical user interface-enabled tool needs to be developed, through which medical practitioners can feed patient...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7093/version/1

Development of new simple sequence repeat markers for pearl millet
Provedor de dados: 30
Autores: Yadav, O.P.
Palavras-chave: Locus; DNA; Genetic structures; PCR; Millets; Polymorphism; Genomes; Biotechnology; Breeds (animals); Melting.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3309

Development of Novel Calibrations for FT-NIR Analysis of Protein, Oil, Carbohydrates and Isoflavones in Foods
Provedor de dados: 17
Autores: I Baianu; Jun Guo.
The development of calibration methodology for novel FT-NIRS analysis of soybean-based foods is presented together with high-precision NIRS spectra and composition measurements in terms of proteins, oil and carbohydrates in soybean-based foods/soy foods.&amp;#xd;&amp;#xa;
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6317/version/1

Diagnostic robustness of DNA microarrays in the classification of acute leukemia
Provedor de dados: 17
Autores: Marie Loh; Adrian Mondry.
Countless statistical methods have been described for the analysis of DNA microarrays, and each yields distinct results. This raises the question whether DNA microarrays are robust diagnostic tools.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;In order to address this issue, we compared five formally similar statistical tests for gene selection on a single data set derived from acute leukemia patients. Inter-test agreement of gene selection, of sample classification and with standard clinical diagnosis was calculated using Cohen&amp;#x27;s &amp;#x3ba;-score.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The inter-test agreement scores were 0.15 &amp;#x3c; &amp;#x3ba; &amp;#x3c; 0.68 for gene selection, and 0.60 &amp;#x3c; &amp;#x3ba; &amp;#x3c; 0.89 for sample classification....
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1056/version/1

Diamond nanowires, a new approach towards next generation electrochemical gene sensor platforms
Provedor de dados: 17
Autores: Nianjun Yang; Hiroshi Uetsuka; Jiuhong Yu; Eiji Osawa; Norio Tokuda; Christoph Nebel.
A novel bio-sensing platform is introduced by combination of a) geometrically controlled DNA bonding using vertically aligned diamond nano-wires and b) the superior electrochemical sensing properties of diamond as transducer material. Ultra-hard vertically aligned diamond nanowires are electrochemically modified to bond phenyl linker-molecules to their tips which provide mesospacing for DNA molecules on the transducer. The nano-wires are generated by reactive ion etching of metallically boron doped atomically smooth single crystalline CVD diamond. Surface properties are characterized by atomic force, scanning electron and scanning tunneling microcopy. Electro- and biochemical sensor properties are investigated using cyclic and differential pulse...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1714/version/1

Different Approaches For Protein Engineering In Industrial Biotechnology
Provedor de dados: 17
Autores: Israr  Khan; Muhammad  Waheed Akhtar.
Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and research. It may apply rational design or non rational design or both. Site directed mutagenesis is a classical approach involving the protein folding principles and as such different techniques involving multidisciplinary research and broad knowledge is required involving biocomputing of complex data obtained from various sequencing projects and prediction of the future protein structure either chemically or genetically modified. Non rational mutagenesis or directed evolution involves random mutations in the gene encoding protein or shuffling the genes encoding different domains producing a random set of numerous large libraries of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/5601/version/1

Differing U.S. and European Perspectives on GMOs: Political, Economic and Cultural Issues
Provedor de dados: 31
Autores: Runge,   C. Ford; Bagnara,   Gian Luca; Jackson,   Lee Ann.
This paper provides an overview of the historical and cultural factors that have contributed to divergent U.S. and European views on GMOs, and to resulting different national regulatory approaches for these products, specifically labelling policy. Within the context of the international trading system, these national policy choices will have impacts that will spill over national borders. Dialogue may be difficult to achieve, given widely divergent views concerning GMOs; however, without dialogue potential global social benefits of policy harmonization will be forfeited.
Tipo: Journal Article
Palavras-chave: Biotechnology; Culture; Harmonization; Labelling; Trade; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/23874

Direct fluorophore conjugation to genomic DNA for microarray-based epigenomic profiling
Provedor de dados: 17
Autores: Vineet Gupta.
A methodology for microarray based epigenomic profiling is presented. The method relies on platinum-based fluorescence labeling reagents for direct (non-enzymatic) labeling of DNA and RNA. This is a work in progress and only preliminary data is presented here.
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/444/version/1

Discovery of transcription factor binding sites through integration of generic motif finders
Provedor de dados: 17
Autores: Edward Wijaya; Siu-Ming Yiu; Thanh Son Ngo; Rajaraman Kanagasabai; Wing-Kin Sung.
Locating transcription factor binding sites is a key step in understanding gene regulation. Due to its importance, many _de novo_ motif-finding methods have been proposed. Individually, these motif finders perform unimpressively overall based on Tompa&amp;#x27;s benchmark datasets. Moreover, these motif finders vary in their definitions of what constitute a motif, and in their methods for finding statistically overrepresented motifs. There is no clear way for biologists to choose the motif finder that is most suitable for their task. The purpose of this work is to describe a method called MotifVoter to identify transcription factor binding sites by integrating the results found by motif finders of different models. Validation of our method on...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1251/version/1

Distancia do fluxo genico ocorrido entre a cultivar de soja BR-16 transgenica e BR-16 nao-transgenica no Cerrado do Distrito Federal.
Provedor de dados: 14
Autores: ABUD, S.; SOUZA, P. I. M.; MOREIRA, C. T.; VIANNA, G. R.; ANDRADE, S. R. M.; RECH, E. L.; ARAGAO, F. J.
bitstream/CPAC-2009/24177/1/p2002_68.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Transgenico; Melhoramento genetico; Transgenics.; Biotecnologia; Genética Vegetal; Glycine Max; Soja.; Biotechnology; Genetics; Plant breeding; Soybeans.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567427

Distribuição da variabilidade genética Stylosanthes macrocephala coletadas na região de cerrado e semi-árido brasileiro.
Provedor de dados: 14
Autores: BARROS, A. M.; FALEIRO, F. G.; KARIA, C. T.; SHIRATSUCHI, L. S.; ANDRADE, R. P.; LOPES, G. K. B.
bitstream/CPAC-2009/26788/1/p2005_25.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: RAPD; Feed legumes.; Biotecnologia; Leguminosa Forrageira; Marcador Genético; Marcador Molecular; Variação Genética.; Genetic variation.; Biotechnology; Genetic markers.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567165

Diversidade genetica de especies silvestres de maracujazeiro com resistencia multipla a doencas com base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; JUNQUEIRA, N. T. V.; BELON, G.; BORGES, T. A.; ANJOS, J. de R. N. dos; PEIXOTO, J. R.; BRAGA, M. F.; SANTOS, D. G.
bitstream/CPAC-2009/24951/1/p2004_05.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: RAPD; Plant diseases.; Biotecnologia; Doença de Planta; Marcador Molecular; Maracujá; Marcador Genético; Passiflora Edulis; Variação Genética; Variedade Resistente.; Biotechnology; Genetic markers; Genetic variation; Disease resistance; Passion fruits.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567929

Diversidade genetica de uma colecao de trabalho de Stylosanthes guianensis com base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; KARIA, C. T.; ANDRADE, R. P.; BARROS, A. M.; SILVA, D. de O. C. e.
bitstream/CPAC-2009/24950/1/p2004_04.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: RAPD; Feed legumes.; Leguminosa Forrageira; Marcador Genético; Marcador Molecular; Melhoramento Genético Vegetal; Variação Genética.; Genetic variation; Genetic markers; Biotechnology; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567917

Diversidade genética de variedades comerciais de maracujazeiro-azedo com base em marcadores moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; JUNQUEIRA, N. T. V.; BRAGA, M. F.; BELLON, G.; PEIXOTO, J. R.
bitstream/CPAC-2009/27708/1/p2006_41.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Biotecnologia; Maracujá; Marcador Molecular; Marcador Genético; Passiflora Edulis; Variação Genética.; Genetic markers; Biotechnology; Genetic variation; Passion fruits.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/544592

DNA expression microarrays may be the wrong tool to identify biological pathways
Provedor de dados: 17
Autores: Adrian Mondry; Marie Loh; Alessandro Giuliani.
DNA microarray expression signatures are expected to provide new insights into patho- physiological pathways. Numerous variant statistical methods have been described for each step of the signal analysis. We employed five similar statistical tests on the same data set at the level of gene selection. Inter-test agreement for the identification of biological pathways in BioCarta, KEGG and Reactome was calculated using Cohen&amp;#x2019;s k- score. The identification of specific biological pathways showed only moderate agreement (0.30 &amp;#x3c; k &amp;#x3c; 0.79) between the analysis methods used.  Pathways identified by microarrays must be treated cautiously as they vary according to the statistical method used.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1036/version/1

DNALinux Virtual Desktop Edition
Provedor de dados: 17
Autores: Sebastian Bassi; Virginia V. C. Gonzalez.
The new version of DNALinux (VDE) is presented. DNALinux VDE is a departure from traditional distributions since it uses a virtual machine to bundle together the operating system and bioinformatics applications. The main advantage of this approach is that a virtualized environment doesn&amp;#x27;t affect a installed system. With a virtual machine a Linux system can be run under a Windows system, provided that the virtual machine player is installed. The included programs are listed and specifications to add more programs are explained. We believe that DNALinux could be used as a standardized virtual machine for learning, using, developing and testing bioinformatics applications.
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/670/version/1

DNA^+Pro^: an Improved Progressive Multiple Sequence Alignment Algorithm for Evolutionary Analysis Using Combined DNA-Protein Sequences
Provedor de dados: 17
Autores: Xiaolong Wang; Shuang-yong  Xu; Deming  Gou.
Alignment of DNA and protein sequences is a basic tool in the study of evolutionary, structural and functional relationship among macromolecules. Present sequence alignment methods are somewhat error-prone, often producing systematic bias. Errors in sequence alignments sometimes lead to subsequent misinterpretation of evolutionary, structural and functional information in genes, proteins and genomes. In traditional sequence alignment algorithms, alignments of DNA and protein sequences are conducted separately. It has been long believed that the phylogenetic signal disappears more rapidly from DNA sequences than from encoded proteins. It is therefore generally preferable to align sequences at the amino acid level. Here we present a new...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4898/version/1

DOA-Maize research and development
Provedor de dados: 106
Autores: Pichet Grudloyma; Somrak Noradechanon; Surapong Prasitwattanaseree; Kalaya papintu; Manoon Pumklom.
9 tables
Palavras-chave: Corn; Maize; Population improvement; Hybrids; Cooperative program; Biotechnology; AMBIONET; Plant breeding; ข้าวโพด; การปรับปรุงประชากร; พันธุ์ลูกผสม; โครงการความร่วมมือ; เทคโนโลยีชีวภาพ; แอมไบโอเนท.
Ano: 2001
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4159

Donor mesenchymal stem cells trigger chronic graft-versus-host disease following minor antigen-mismatched bone marrow transplantation
Provedor de dados: 17
Autores: Yoko Ogawa; Satoru Morikawa; Hideyuki Okano; Yo Mabuchi; Sadafumi Suzuki; Tomonori Yaguchi; Saori Yaguchi; Takaaki Inaba; Shinichiro Okamoto; Yutaka Kawakami; Kazuo Tsubota; Shigeto Shimmura; Yumi Matsuzaki.
Chronic graft-versus-host disease (cGVHD) is a complication after minor antigen mismatched bone marrow transplantation (BMT) characterized by an autoimmune-type reaction in various organs. Aberration in T cell regulation is involved, with donor mesenchymal stem cells (MSCs) playing a possible role in immunomodulation. In a minor-antigen mismatched mouse BMT model, transplantation of mismatched, but not syngeneic MSCs triggered the onset of cGVHD, and was associated with fibrosis, increased IL-6 secretion, decreased Foxp3+ regulatory T cells and increased Th17 in the peripheral blood. Mismatched MSCs alone were sufficient to induce cGVHD, while removal of donor MSCs rescued mice from cGVHD. RAG2 knockout recipient mice did not suffer cGVHD, indicating that...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology.
Ano: 2012
URL: http://precedings.nature.com/documents/6843/version/1

Drugs obtained by biotechnology processing
Provedor de dados: 60
Autores: Almeida,Hugo; Amaral,Maria Helena; Lobão,Paulo.
In recent years, the number of drugs of biotechnological origin available for many different diseases has increased exponentially, including different types of cancer, diabetes mellitus, infectious diseases (e.g. AIDS Virus / HIV) as well as cardiovascular, neurological, respiratory, and autoimmune diseases, among others. The pharmaceutical industry has used different technologies to obtain new and promising active ingredients, as exemplified by the fermentation technique, recombinant DNA technique and the hybridoma technique. The expiry of the patents of the first drugs of biotechnological origin and the consequent emergence of biosimilar products, have posed various questions to health authorities worldwide regarding the definition, framework, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Recombinant DNA; Hybridoma technique; Bacteria; Antibiotics; Vaccines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200002

Dual Diode Array and Fourier Transform Near Infrared Reflectance Spectrometer Calibrations for Composition Analysis of Single Soybean Seeds for Genetic Selection, Cross-Breeding Experiments
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Randall  L. Nelson.
Experimental results of extensive evaluations of Diode-Array and FT-NIRS instruments are presented that aim at developing reliable and robust NIR calibrations for single soybean seed composition analysis on both Dual Diode Array and Fourier Transform NIR reflectance instruments. Single soybean seed, bulk, and powder calibrations were developed on four different NIRS spectrometer models that are commercially available.
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6195/version/1

Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells
Provedor de dados: 17
Autores: Jian-Ping Lu; Laura Beatty; Jehonathan  Pinthus.
Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary for protein-targeted therapy. Evidently high throughput formatting of these applications in living cells would be beneficial, however no adequate system exists. We present a novel platform technology for the high throughput screening and verification of protein interactions in living cells. The platform&amp;#x2019;s series of Dual Expression Recombinase Based (DERB) destiny vectors individually encode two sets of recombinase recognizable sequences for inserting the protein open reading frame (ORF) of interest, two sets of promoters and reporter tags in frame with the ORFs for detecting interactions. Introduction into living...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1550/version/2

Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells
Provedor de dados: 17
Autores: JianPing Lu; Laura Beatty; Jehonathan Pinthus.
Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary for protein-targeted therapy. Evidently high throughput formatting of these applications in living cells would be beneficial, however no adequate system exists. We present a novel platform technology for the high throughput screening and verification of protein interactions in living cells. The platform&amp;#x27;s series of Dual Expression Recombinase Based (DERB) destiny vectors individually encode two sets of recombinase recognizable sequences for inserting the protein open reading frame (ORF) of interest, two sets of promoters and reporter tags in frame with the ORFs for detecting interactions. Introduction into living...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1550/version/1

Early Diagnosis of Alzheimer&amp;#x27;s disease by NIRF Spectroscopy  and Nuclear Medicine
Provedor de dados: 17
Autores: I. C. Baianu.
There is an urgent need for the early detection of diseases such as Alzheimer&amp;#x2019;s (AD) and Cancers in order to enable their successful treatment. Cancer is the second major cause of death after Heart Disease, and AD is the third major cause of death with major, human and financial/economics trillion dollar consequences for the society. Nuclear Medicine is concerned with applications in Medicine of Nuclear Science and Engineering techniques and knowledge. Three major Nuclear Medicine techniques that are established for diagnostic and research purposes are: Positron Emission Tomography (PET) and CAT/CT, Nuclear Magnetic Resonance Imaging (NMRI/MRI). However, these three techniques have also major limitations in terms of either cost or image...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics &amp; Genomics; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6271/version/1

Early Diagnosis of Alzheimer&amp;#x27;s disease by NIRF Spectroscopy  and Nuclear Medicine-v.4.0
Provedor de dados: 17
Autores: I Baianu.
There is an urgent need for the early detection of diseases such as Alzheimer&amp;#x2019;s (AD) and Cancers in order to enable their successful treatment. Cancer is the second major cause of death after Heart Disease, and AD is the third major cause of death with major, human and financial/economics trillion dollar consequences for the society. Nuclear Medicine is concerned with applications in Medicine of Nuclear Science and Engineering techniques and knowledge. Three major Nuclear Medicine techniques that are established for diagnostic and research purposes are: Positron Emission Tomography (PET) and CAT/CT, Nuclear Magnetic Resonance Imaging (NMRI/MRI). However, these three techniques have also major limitations in terms of either cost or image...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6273/version/1

ECoG-based short-range recurrent stimulation techniques to stabilize tissue at risk of progressive damage:  Theory based on clinical observations
Provedor de dados: 17
Autores: Markus A. Dahlem; Felix M. Schneider; Jens P. Dreier; Anthony J. Strong; Eckehard Schoell.
We introduce theoretical concepts based on chaos control to stabilize in acute stroke the tissue at risk of progressive damage by preventing adverse effects of waves of mass neuronal depolarization.  Moreover, we present clinical electrocorticography (ECoG) recordings of relevant signals suggested for the feedback control. The recordings are performed in combination with novel subdural opto-electrode technology for simultaneous laser-Doppler flowmetry in patients with aneurysmal subarachnoid haemorrhage (aSAH). In aSAH patients waves of spreading depolarization (SD) have a high incidence and cause hypoxia in tissue at risk, and, importantly, the haemodynamic response is the inverse of that seen in healthy tissue. In previous clinical studies, clusters of...
Tipo: Poster
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3408/version/1

Ecological, phenological and biochemical significance of bimodal fruiting of Boletus alutaceus morgan
Provedor de dados: 17
Autores: Nandkumar Kamat.
Boletus alutaceus Morgan an ectomycorrhizal species was extensively studied from an ecologically fragmented microhabitt colonized by its&amp;#x27; host plant Ficus benghalensis L. in the Goa University campus, Taleigao, Goa, India. Qualitatively the fruiting cycles were monitored for three years and thereafter detail ecological and morphological investigations were carried out in May and November 2004. A bimodal temporal fruting pattern emerged which may have significant ecological, phenological and biochemical role in EM dynamics.
Tipo: Presentation
Palavras-chave: Biotechnology; Developmental Biology; Ecology; Genetics &amp; Genomics; Microbiology; Plant Biology; Evolutionary Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/2835/version/1

Ecological Risks of Novel Environmental Crop Technologies Using Phytoremediation as an Example
Provedor de dados: 31
Autores: Angle,   J. Scott; Linacre,   Nicholas A.
Phytoremediation is the use of living plants, known as hyperaccumulators which absorb unusually large amounts of metals in comparison to other plants. The use of classical plant breeding and new molecular techniques offers great potential to develop crops with the ability to clean up polluted sites. While these technologies have gained widespread attention, prior to commercial development, there are risks that must be considered – only a few of which have received even modest examination. Therefore, the focus of this working paper is to explore specific risks associated with phytoremediation and suggest ways in which these risks can be managed so that new, novel, and innovative plant technologies may be applied to provide low cost and efficient...
Tipo: Working or Discussion Paper
Palavras-chave: Risk; GMO; Biotechnology; Phytoremediation; Phytoextraction; Phytomining; Environmental risk; Crop Production/Industries; Q16; Q2.
Ano: 2005
URL: http://purl.umn.edu/59225

Economic Benefits and Costs of Biotechnology Innovations in Agriculture
Provedor de dados: 31
Autores: Moschini,   GianCarlo.
The conceptual model necessary for an assessment of biotechnology's economic benefits and costs is outlined, emphasizing the need to account for the proprietary nature of biotechnology innovations. The model is illustrated with an application to Roundup Ready soybeans. The estimated value of this innovation is sizeable, with consumers and innovators claiming the larger share of net benefits. Also, disparities in intellectual property rights protection across countries affect the distribution of benefits. Consumer resistance toward genetically modified organisms (GMOs) and the issues of labeling and market segregation complicate the economic evaluation of biotechnology innovations, and a number of related regulation and public policy issues are discussed....
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Genetically modified organisms; Identity preservation; Intellectual property rights; R&amp;D; Transgenic crops; Welfare evaluation; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/18413

ECONOMIC IMPACTS OF GENETICALLY MODIFIED CROPS IN CHINA
Provedor de dados: 31
Autores: Huang,   Jikun; Hu,   Ruifa; van Meijl,   Hans; van Tongeren,   Frank W.
China has made a major investment in biotechnology research.  Genetically modified (GM) cotton is widely adopted and the list of GM technologies in trials is impressive.  At the same time there is an active debate on when China should commercialize its GM food crops.  The overall goal of this paper is to provide an economy-wide assessment of these issues under various scenarios.  Based on a unique data from empirical micro-level study and field trial in China and a modified GTAP model, our results indicate that the development of biotechnology has an important impact on China's production, trade and welfare.  Welfare gains far outweigh the public biotechnology research expenditures.  Most gains occur inside China.  Policy makers should put less weight on...
Tipo: Conference Paper or Presentation
Palavras-chave: Economic impacts; Biotechnology; Genetically modified crops; China; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies; C68; D58; F13; O33; Q17; Q18.
Ano: 2003
URL: http://purl.umn.edu/25883

ECONOMIC ISSUES IN AGRICULTURAL BIOTECHNOLOGY
Provedor de dados: 31
Autores: Shoemaker,   Robbin A.; Harwood,   Joy L.; Day-Rubenstein,   Kelly A.; Dunahay,   Terry; Heisey,   Paul W.; Hoffman,   Linwood A.; Klotz-Ingram,   Cassandra; Lin,   William W.; Mitchell,   Lorraine; McBride,   William D.; Fernandez-Cornejo,   Jorge.
Agricultural biotechnology has been advancing very rapidly, and while it presents many promises, it also poses as many questions. Many dimensions to agricultural biotechnology need to be considered to adequately inform public policy. Policy is made more difficult by the fact that agricultural biotechnology encompasses many policy issues addressed in very different ways. We have identified several key areas — agricultural research policy, industry structure, production and marketing, consumer issues, and future world food demand — where agricultural biotechnology is dramatically affecting the public policy agenda. This report focuses on the economic aspects of these issues and addresses some current and timely issues as well as longer term issues.
Tipo: Report
Palavras-chave: Biotechnology; Economics; Adoption; Patents; Research policy; Markets; Market segmentation; Identity preservation; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/33735

Effect of Escherichia coli endotoxin on Archachatina marginata haemolymph coagulation system
Provedor de dados: 17
Autores: Musa Salawu; Oyelola Oloyede; Adenike Oladiji; Nasiru Muhammad; Musa Yakubu.
The effect of _E. coli_ endotoxin on the heamolymph coagulation response of _Archachatina marginata_ was studied. Heamocyte Lysate(HL), Haemocyte Lysate Supernatant(HLS) and Haemocyte Lysate Debris (HLD) were exposed to _Escherichia coli_ endotoxin. Controls were prepared with endotoxin-free water(&amp;#x3c;0.025 EU/ml). The differential protein coagulation was estimated in each mixture. Fractions of the haemolymph exposed to endotoxin produced higher protein coagulates than endotoxin-free fractions when incubated at 37 &amp;#xb0;C for 1 h (p&amp;#x3c;0.05). The results showed significantly higher (p&amp;#x3c;0.05) concentrations of protein coagulated when HL/plasma mixture were used than when either fraction was used. At a ratio 1:1 of HL:Plasma, highest...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4552/version/1

Effect of In Ovo Feeding of L-Glutamine to Chick Embryos
Provedor de dados: 89
Autores: Rufino,JPF; Cruz,FGG; Costa,VR; Silva,AF; Melo,LD; Bezerra,NS.
ABSTRACT In ovo feeding (IOF), injecting nutrients into the amnion of the avian embryo may enhance hatchability, gastrointestinal development and serum metabolism changes. This hypothesis was evaluated with 5 IOF solutions containing L-glutamine. Were used 315 fertile Rhode Island Red eggs were used (breeders with 32-weeks). The experimental design was completely randomized with the treatments constituted by two controls and five solutions containing L-glutamine levels with 45 replicates each. Data collected were subjected to polynomial regression at 5% of significance. Differences (p&gt;0.05) were observed in hatchability and intermediary mortality, with a gradual lower of decrease in hatchability from the IOF of L-glutamine. The yolk sac was higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aminoacid; Biotechnology; Embryo mortality; Hatchability; In ovo feeding.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400300

Effect of Stimulating Magenetic Field on Plants
Provedor de dados: 17
Autores: R. V. K.  Charan.
A plant&amp;#x27;s metabolism contains 90-95 percent of diamagnetic water and rest contains several apra, ferro and dia metals and non-metals.The present work is to study stimulating magnetic effect on plants. In result I find abrupt rising of the pulse rate which may enhance the growth of the plant resulting in removing of necessity of using fertilizers and chemicals for plant growth if it is for human consumption. A relation can also be established in between plant pulsation and the applied field.
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3300/version/1

Effectiveness of both synthetic compounds and biological extracts against powdery mildew (Blumeria graminis f. sp. tritici) on winter wheat
Provedor de dados: 115
Autores: Věchet,Lubomír; Šerá,Božena.
The objective of this study was to evaluate the effectiveness of foliar application of various synthetic compounds and biological extracts against powdery mildew on wheat. Seed coats of winter wheat were treated by synthetical compounds (benzothiadiazole, salicylic acid, and glycine betaine) or by the extracts from plants (oak, giant knotweed, curcuma, and ginger). Then the seeds were planted using small-field experiments with powdery mildew infection (repetitions over a two-year period; experimental field in Ruzyne, Prague). Leaf area infection was measured and the Cumulative Proportion of Leaf Area Diseased (CPLAD) was calculated. Data were analysed using ANOVA (Tukey test, p≤0.05). Effectiveness of preparations varied 30 % to 72 % in 2008, and 25 % to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Curcuma; Giant knotweed; Ginger; Infection; Oak.
Ano: 2015
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000100006

Effects of Bt Cotton in India During the First Five Years of Adoption
Provedor de dados: 31
Autores: Sadashivappa,   Prakash; Qaim,   Matin.
While previous research has analyzed the impacts of Bt cotton in India, most available studies are based on one or two years of data only. We analyze the technology’s performance over the first five years of adoption, using panel data with three rounds of observations. On average, Bt adopting farmers realize pesticide reductions of about 40%, and yield advantages of 30-40%. Profit gains are in a magnitude of US $60 per acre. These benefits have been sustainable over time. Farmers’ satisfaction is reflected in a high willingness to pay for Bt seeds. Nonetheless, in 2006 Indian state governments decided to establish price caps at levels much lower than what companies had charged before. This intervention has further increased farmers’ profits, but the impact...
Tipo: Journal Article
Palavras-chave: Biotechnology; Bt cotton; Genetically modified crops; Farm survey; India; Seed markets; Technology adoption; Willingness to pay (WTP); Environmental Economics and Policy; Research and Development/Tech Change/Emerging Technologies; O32; O33; Q16; Q55; Q58.
Ano: 2009
URL: http://purl.umn.edu/49947

Effects of Seed and Farm Characteristics on Cottonseed Choice:  A Choice-Based Conjoint Experiment in the Mississippi Delta
Provedor de dados: 31
Autores: Banerjee,   Swagata (Ban); Hudson,   Darren; Martin,   Steven W.
Producers’ preferences for cottonseed with respect to price, seed type, yield, and fiber quality are examined by a willingness-to-pay approach via mail surveys.  Results indicate a positive willingness to pay (WTP) for technology relative to conventional cottonseed, and WTP increases with the level of technology.  Yield and quality also show a positive WTP.  Larger farms have a higher WTP for technology, and farms with more farm labor have a lower WTP for technology.  These results suggest economies of size in technology adoption (biotechnology is not size-neutral) and that labor and biotechnology are direct substitutes.
Tipo: Journal Article
Palavras-chave: Biotechnology; Conjoint analysis; Conjoint (choice) experiment; Cotton; Farm labor; Farm size; Fiber quality; Willingness to pay (WTP); D24; Q12; Q16.
Ano: 2007
URL: http://purl.umn.edu/37054

Effects of temperature and time on oil extraction from some Nigerian indigenous fresh water microalgae species
Provedor de dados: 111
Autores: Asoiro, Felix Uzochukwu; Okonkwo, Wilfred Ifeanyi; Akubuo, Clement Onyeaghala; Nweze, Nkechinyere O.; Anyanwu, Cosmas Ngozichukwu.
This study determined the effects of temperature and time on oil extraction from indigenous freshwater microalgae– Dictyosphaerium, Chlorella, Desmodesmus and Cosmarium species, cultured in fifteen 2-litre column photobioreactors (PBR) (three per specie).  Growth and specific growth rates for the mixotrophic&gt;autotrophic cultivation of the species. Oil was extracted from the dried microalgae species at temperatures ranging from 40 to 120 °C at 20 oC intervals and times ranging from 30 to 210 minutes at 30 minutes’ intervals, by accelerated solvent extraction method. Extraction temperature, time and type of microalgae species had significant effect (p &lt; 0.05) on oil yield (temperature &gt; time &gt; type of species).  As extraction temperature and time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agricultural engineering; Bioresources engineering; Biotechnology; Energy; Renewable energy; Agriculture. oil yield; Dictyosphaerium spp; Desmodesmus subspicatus; Chlorella lewinii; Cosmarium spp; Growth rate.
Ano: 2019
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5247

Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells.
Provedor de dados: 17
Autores: Nathan P. Gabrielson; Daniel W. Pack.
Most in vivo gene therapies will require cell-specific targeting. Although vector targeting through ligand attachment has met with success in generating gene delivery particles that are capable of specific cellular interactions, little attention has been given to the possible effects of such ligands on subsequent intracellular processing. In this study, we examine the impact of targeting two distinct endocytic routes-the caveolar and clathrin pathways-on polyethylenimine-mediated gene delivery in HeLa cells. Targeting complexes to the caveolar pathway with folic acid and the clathrin pathway with transferrin yields enhanced gene delivery relative to unmodified polyethylenimine. Colocalization studies with caveolin-1 and clathrin heavy chain indicate that...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer.
Ano: 2008
URL: http://precedings.nature.com/documents/2262/version/1

Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant
Provedor de dados: 39
Autores: Magioli,Claudia; Mansur,Elisabeth.
Eggplant is an agronomically important non-tuberous solanaceous crop grown primarily for its large oval fruit. In popular medicine, eggplant is indicated for the treatment of several diseases, including diabetes, arthritis, asthma and bronchitis. Eggplant is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by hybridizing eggplant with wild resistant Solanum species, which present a wide genetic diversity and are source of useful agronomic traits. The application of in vitro methodologies to eggplant has resulted in considerable success. Eggplant tissues present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum melongena L.; Biotechnology; Tissue culture; Genetic transformation; Model plant.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062005000100013

Embryogenic calli induction from nucellar tissue of Citrus cultivars
Provedor de dados: 63
Autores: Mendes-da-Glória,Fernanda Januzzi; Mourão Filho,Francisco de Assis Alves; Demétrio,Clarice Garcia Borges; Mendes,Beatriz Madalena Januzzi.
Nucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1)]; 1/2-EME [half concentration of MT macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA [EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins (Citrus reticulata, Blanco), 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck), 'Serra d'água' and 'Valencia' sweet oranges (Citrus sinensis, L. Osbeck) 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L. Osbeck) produced hard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Tissue culture; Cultivar improvement; Growth regulator; Protoplast.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500012

Emergence and Fixing of Antiviral Resistance in Influenza A Via Recombination and Hitch Hiking
Provedor de dados: 17
Autores: Henry L. Niman.
The dramatic rise of oseltamivir resistance in the H1N1 serotype in the 2007/2008 season and the fixing of H274Y in the 2008/2009 season has raised concerns regarding individuals at risk for seasonal influenza, as well as development of similar resistance in the H5N1 serotype.  Previously, oseltamivir resistance produced changes in H1N1 and H3N2 at multiple positions in treated patients.  In contrast, the recently reported resistance involved patients who had not recently taken oseltamivir.  Moreover, the resistance was limited to the H1N1 which had acquired H274Y.  Using phylogenetic analysis I show that the fixing of H274Y was due to hitch hiking on a genetic background that acquired key changes from another circulating sub-clade.  H274Y jumped from...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Immunology.
Ano: 2009
URL: http://precedings.nature.com/documents/2832/version/1

Emerging Trends of Nanotechnology towards Picotechnology: Energy and Biomolecules
Provedor de dados: 17
Autores: Rakesh Sharma; Avdhesh Sharma; Ching J. Chen.
In nature, metal oxide particles display their existence at the level of picomolecules in solution state and bioactive states in the body. We present evidence of picomolar behavior of molecules different than nanomolar behavior of particles. These particles can be encapsulated in polymers and can be functionalized with protein, nucleotides, and drugs to develop as smart intracellular targeting pico-devices. The preparation technique and physiological conditions decide the size and functionality of these pico-carrier devices. Their usable success rate, feasibility and potentials are yet to be proven or we do not know. The major difference between nanodevices and pico-devices is their intermolecular and intramolecular thermodynamics in medium and their...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4525/version/1

Empirical predictions of an intrinsically disordered protein theory approach to glycan/lectin reaction kinetics
Provedor de dados: 17
Autores: Rodrick Wallace.
Newly-developed methods from the theory of intrinsically disordered proteins can be applied to the flexible glycan structures that coat cellular surfaces and provide rich channels for biological information transmission. Extension of a mechanistic &amp;#x27;arm-in-sleeve&amp;#x27; model via a nonrigid molecule symmetry analysis leads to expectation of empirical observation of punctuated &amp;#x27;spectral&amp;#x27; classifications in glycan/lectin interaction, parameterized by an appropriate index of glycan frond length or other index of topological complexity, possibly requiring groupoid classifications analogous to quasicrystals.
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6752/version/1

EMPLOYMENT IN BIOTECHNOLOGY IN GERMANY
Provedor de dados: 31
Autores: Menrad,   Klaus.
Paper prepared for presentation at the 8th ICABR International Conference on Agricultural Biotechnology: International Trade and Domestic Production Ravello (Italy), July 8 to 11th, 2004
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Employment; Germany; Application industries; Simulation; Labor and Human Capital; E24; J21.
Ano: 2004
URL: http://purl.umn.edu/91012

Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10
Provedor de dados: 5
Autores: Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...
Tipo: Text
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf

Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10
Provedor de dados: 5
Autores: Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.
A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...
Tipo: Text
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00372/48356/48545.pdf

Enhancing me - hope or hype?
Provedor de dados: 17
Autores: Pete Moore.
Many people talk about human enhancement as if it is about to happen. Some suggest that it already has. To join in the debate you need to distinguish between social, personal and species enhancement. Social and personal are certianly with us - but talk of species enhancement leans more to hype than hope.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2139/version/1

Enhancing RNAi efficiency by inserting Nuclear factor-&amp;#x3ba;B binding sequence into SiRNA expression cassette
Provedor de dados: 17
Autores: Jianzhong Yi; Chenqian Liu.
RNAi based applications have been widely used to manipulate cellular phenotypes, analyze gene function and as a promising molecular therapy, however, there are rare reports in how to target the SiRNA expression constructs from the cytoplasm to nucleus. In this study, we inserted NF-&amp;#x3ba;B binding sequence into the SiRNA expression cassette to enhance RNAi efficiency, tested this propose by use of tRNAval and human H1 promoters, and eGFP and luciferase as reporter protein. The results showed that inserting NF-&amp;#x3ba;B binding sequence at the 3&amp;#x2019;-terminal of the SiRNA expression construct could enhance RNAi efficiency.
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4283/version/1

Enzyme activity and dynamics in near-anhydrous conditions
Provedor de dados: 17
Autores: Murielle Lopez; Vandana Kurkal-Siebert; Rachel Dunn; Moeava Tehei; John Finney; Jeremy Smith; Roy Daniel.
Water is widely assumed to be essential for life 1, although the exact molecular basis of this requirement is unclear 2-4. Water facilitates protein motions 5-9 and although enzyme activity has been demonstrated at low hydrations in organic solvents 10-13, such non-aqueous solvents may allow the necessary motions for catalysis. To examine enzyme function in the absence of solvation and bypass diffusional constraints we have tested the ability of an esterase to catalyse alcoholysis as an anhydrous powder, using a closed reaction system in which the substrates and products of the enzyme reaction are gaseous 14-15, and where the water content can be well defined 16. At hydrations equivalent to 3 (&amp;#xb1;2) molecules of water per molecule of enzyme,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/3884/version/1

ESG: Extended Similarity Group method for automated protein function prediction
Provedor de dados: 17
Autores: Meghana Chitale; Troy Hawkins; Changsoon Park; Daisuke Kihara.
We present here the Extended Similarity Group (ESG) method, which annotates query sequences with Gene Ontology (GO) terms by assigning probability to each annotation computed based on iterative PSI-BLAST searches. Conventionally sequence homology based function annotation methods, such as BLAST, retrieve function information from top hits with a significant score (E-values). In contrast, the PFP method, which we have presented previously, goes one step ahead in utilizing a PSI-BLAST result by considering very weak hits even an E-value of up to 100 and also by incorporating the functional association between GO terms (FAM matrix) computed using term co-occurrence frequencies in the UniProt database. PFP is very successful which is evidenced by the top rank...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2193/version/1

Establishing a multicellular model by three-dimensional cell-assembly technique for metabolic syndrome
Provedor de dados: 17
Autores: Mingen Xu; Yongnian Yan; Renjie Zhang; Ri Yao; Haixia Liu; Xiaohong Wang.
One of the major obstacles in developing multifunctional drugs for the metabolic syndrome (MS) is lack of in vitro models that capture more complex features of the disease. Here we give the first report that establishes an energy metabolic system model using cell-assembly technique which can assemble cells into designated places to form complex three-dimensional structures. Adipose-derived stromal cells were assembled and induced differentiation into adipocytes and endothelial cells; pancreatic islets were then deposited at designated locations and constituted adipoinsular axis with adipocytes. Analysis of the factors involved in energy metabolism showed our system could capture more physiological and pathophysiological features of the in vivo energy...
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/1496/version/1

Estimate of the Potential Adoption of Genetically Modified Cotton by Farmers in Southern Spain and its Economic Implications
Provedor de dados: 31
Autores: Gomez-Barbero,   Manuel; Rodgriguez-Cerezo,   Emilio.
This paper analyses the potential adoption and economic on-farm impact of Bt cotton in the Andalusia region (southern Spain). Survey data have shown that, on average, 58% of the responding farmers know about genetically modified (GM) cotton. Another outcome of the survey is that 95% of the farmers declaring that they know about GM cotton would be willing to grow Bt cotton. In the main cotton-producing districts, farmers' attitudes are positive, and they would be willing to adopt the technology. In areas where the crop is more important, the farmers who are willing to adopt Bt cotton represent over 75% of the area under cotton. As regards the on-farm economic impact resulting from a potential adoption, the assessment is that savings on the cost of direct...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Cotton; Economic impact; Andalusia; Agricultural and Food Policy; Q16; Q20; Q12; O52.
Ano: 2005
URL: http://purl.umn.edu/24556

Estimating the Adoption of Bt Eggplant in India: Who Benefits from Public-Private Partnership?
Provedor de dados: 31
Autores: Krishna,   Vijesh V.; Qaim,   Matin.
The study analyzes ex-ante the adoption of insect resistant Bt eggplant in India. Farmers' willingness to pay (WTP) is estimated using the contingent valuation method. Given the economic importance of insect pests, the average WTP for proprietary Bt hybrids is more than four times the current price of conventional hybrids. Since the private innovating firm has shared its technology with the public sector, it is likely that public open-pollinated Bt varieties will also be released after a small delay. This will reduce farmers' WTP for Bt hybrids by 35%, thus decreasing the scope for corporate pricing policies. Nonetheless, ample profit potential remains. Analysis of factors influencing farmers' adoption decisions demonstrates that public Bt varieties will...
Tipo: Conference Paper or Presentation
Palavras-chave: Public-private partnership; Biotechnology; Bt eggplant; Adoption; Willingness to pay; India; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2006
URL: http://purl.umn.edu/25311

Estimation of extracellular lipase enzyme produced by thermophilic bacillus sp. isolated from arid and semi-arid region of Rajasthan, India
Provedor de dados: 17
Autores: Deeksha   Gaur; Pankaj Kumar   Jain; Yamini Singh  Sisodia; Vivek  Bajpai.
Thermophilic organisms can be defined as microorganisms which are adapted to live at high temperatures. The enzymes produce by thermophilic bacteria are capable of catalyzing biochemical reactions at high temperatures. Thermophilic bacteria are able to produce thermostable lipase enzymes capable of degradation of lipid at temperatures higher than those of mesophilic bacteria. Therefore, the isolation of thermophilic bacteria from natural sources and their identification are quite useful in terms of discovering thermophilic lipase enzymes. Due to great temperature fluctuation in hot arid and semi-arid regions of Rajasthan, this area could serve as a good source for new thermophilic lipase producing bacteria with novel industrially important properties. The...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology.
Ano: 2012
URL: http://precedings.nature.com/documents/7072/version/1

Estudo bibliométrico e análise de patentes acerca da produção de ácidos carboxílicos e álcoois a partir de açúcares: parte 1: ácidos carboxílicos.
Provedor de dados: 14
Autores: BRAGA, M.; FERREIRA, P. M.; LOPES, C. L.; BELÉM, D. DE L.; ALMEIDA, J. R. M. de.
Esta publicação é o primeiro produto do trabalho do Setor de Prospecção e Avaliação de Tecnologias (SPAT) da Embrapa Agroenergia e trata da plataforma industrial de açúcares C5/C6, mais especificamente sobre as principais classes de produtos derivados dessas matérias-primas: os ácidos carboxílicos e álcoois. O objetivo geral do trabalho é determinar, com o uso de ferramentas bibliométricas, o cenário tecnológico atual da produção de ácidos carboxílicos (Parte 1) e álcoois (Parte 2), a partir da biomassa lignocelulósica, bem como avaliar o cenário mercadológico dos produtos em questão.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Ácidos carboxílicos; Álcoois de cadeia curta; Biomassa lignocelulósica; Bibliometria; Biotecnologia; Biomassa; Mercado; Biotechnology; Biomass; Carboxylic acids; Alcohols; Market analysis.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1131165

Estudo bibliométrico e análise de patentes acerca da produção de ácidos carboxílicos e álcoois a partir de açúcares: parte 2: álcoois.
Provedor de dados: 14
Autores: BRAGA, M.; FERREIRA, P. M.; OLIVEIRA, C. R. de; ALMEIDA, J. R. M. de.
Este trabalho, juntamente com o Documentos 36 (Estudo bibliométrico e análise de patentes acerca da produção de ácidos carboxílicos e álcoois a partir de açúcares - Parte 1: Ácidos carboxílicos) formam o mais completo estudo sobre a utilização da biomassa para a produção de bioquímicos renováveis realizado pela Embrapa Agroenergia. Nesta segunda parte, o tema explorado é a produção de álcoois a partir de fontes renováveis de matéria-prima. Semelhante ao que foi realizado na parte 1, são analisados dados e informações recuperadas em fontes secundárias, especialmente publicações científicas e patentárias, com o objetivo de traçar um panorama da pesquisa científica e aplicada acerca da obtenção de álcoois derivados da biomassa, bem como determinar o cenário...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Biotecnologia; Biomassa; Mercado; Biotechnology; Biomass; Carboxylic acids; Alcohols; Market analysis.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1131537

Eutils-search - manual do usuário.
Provedor de dados: 14
Autores: HIGA, R. H.; YASUDA, R. S.
O software Eutils-search tem por objetivo trazer do banco de dados PubMed informações sobre artigos relacionados a genes de um organismo específico, de acordo com as regras referentes à taxa de acesso impostas pelo site. As informações trazidas são, então, armazenadas localmente em um banco de dados para acesso rápido. Além disso, o software também gera documentos XML correspondentes às informações do organismo requisitado. O eutils-search é uma ferramenta de apoio ao desenvolvimento de aplicações de mineração de textos voltadas para os domínios de biotecnologia e biologia molecular, baseada em informações textuais obtidas do banco de dados PubMed. Este documento apresenta os pré-requisitos e a descrição dos parâmetros necessários para utilização do...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Software eutils-search; Mineração de textos; Software.; Biologia Molecular; Biotecnologia.; Biotechnology; Information retrieval.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/883643

Eutils-search versão 2.0 - manual do usuário.
Provedor de dados: 14
Autores: TANAKA, R. S.; HIGA, R. H.
O software Eutils-search tem por objetivo trazer do banco de dados PubMed informações sobre artigos relacionados a genes de um organismo específico, de acordo com as regras referentes à taxa de acesso impostas pelo site. As informações trazidas são, então, armazenadas localmente em um banco de dados para acesso rápido. Além disso, o software também gera documentos XML correspondentes às informações do organismo requisitado. O eutils-search é uma ferramenta de apoio ao desenvolvimento de aplicações de mineração de textos voltadas para os domínios de biotecnologia e biologia molecular, baseada em informações textuais obtidas do banco de dados PubMed. Este documento apresenta os pré-requisitos e a descrição dos parâmetros necessários para utilização do...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Software eutils-search; Mineração de texto; Text mining; Software.; Biotecnologia.; Biologia Molecular; Biotechnology; Molecular biology.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/920856

Evaluation of effective parameters on fabrication of BSA nanoparticles
Provedor de dados: 17
Autores: Mostafa Rahimnejad; Ghasem Najafpour; Mohsen Jahanshahi.
Coacervation technique was implemented in fabrication of BSA nanoparticle for the purpose of drug delivery system. Nanoparticle was purified with 48,800 g centrifuge, and then followed by dialysis, micro and ultra filtration. The fabricated nanoparticles were subjected to physical, morphological and biochemical characterization. The analyzed samples by SEM and AFM proved the semi-spherically shape of the particles whilst the size distribution was measured by photon correlation spectroscopy. Fourier transforms infra-red spectroscopy (FTIR) spectrum indicated that the fabricated nanoparticles were pure. The impact of various parameters upon the particle size was extensively investigated. The concentration of glutaraldehyde at fixed temperature had absolutely...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Evolutionary Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/2966/version/1

Evaluation of protein surface roughness index using its heat denatured aggregates
Provedor de dados: 17
Autores: Hrishikesh Mishra; Tapobrata Lahiri.
Recent research works on potential of different protein surface describing parameters to predict protein surface properties gained significance for its possible implication in extracting clues on protein&amp;#x27;s functional site. In this direction, Surface Roughness Index, a surface topological parameter, showed its potential to predict SCOP-family of protein. The present work stands on the foundation of these works where a semi-empirical method for evaluation of Surface Roughness Index directly from its heat denatured protein aggregates (HDPA) was designed and demonstrated successfully. The steps followed consist, the extraction of a feature, Intensity Level Multifractal Dimension (ILMFD) from the microscopic images of HDPA, followed by the mapping of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3693/version/1

Evolving standards and infrastructure for systems biology: SBML, SBGN, and BioModels.net
Provedor de dados: 17
Autores: Michael Hucka.
Systems biology has arisen through the convergence of theoretical, computational, and mathematical modeling of systems and the need to understand the wealth of information being rapidly generated in biology.  Systems biology by its nature requires collaborations between scientists with expertise in biology, chemistry, computer sciences, engineering, mathematics, and physics.  Successful integration of these disciplines depends on bringing to bear both social and technological tools: namely, consortia that help forge collaborations and common understanding, software tools that permit analysis of vast and complex data, and agreed-upon standards that enable researchers to communicate and reuse each other&amp;#x27;s results in practical and unambiguous ways....
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2007
URL: http://precedings.nature.com/documents/20/version/1

Exchanging Experimental Kinetic Data via SabioML
Provedor de dados: 17
Autores: Martin Golebiewski.
The simulation of quantitative biochemical models not only requires qualitative information about the stoichiometry of the described networks, but also kinetic data describing their dynamics. Such kinetic data have to be experimentally measured, collected, systematically structured and stored to finally make them accessible. However, the dataflow from the experiment to the model is still a bottleneck, calling for systems that capture the data directly from the instrument, process and normalize it to agreed standards and finally transfer the data to publicly available databases.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;SABIO-RK (http://sabio.h-its.org) is a curated database system which we have developed for bundling data referring to biochemical reactions and...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5030/version/1

Experimental and theoretical study of DNA oxidation
Provedor de dados: 17
Autores: Yuanzhi Song.
The electrochemical oxidation of DNA and bases at a glassy carbon electrode were investigated using cyclic and square-wave voltammetry analysis. The predicted oxidative potentials for DNA and bases using DFT method (B3LYP) are in agreement with the experimental ones.The DNA oxidation ascribes to N-protonated guanine and adenine. The calculated oxidative peaks indicate that the initial potential of DNA oxidation is around 0.432V for N-protonated guanine and 0.368V (vs.hydrogen standard electrode) at pH 7.00, and DNA as well as bases in acidity solution are more stable than those in alkalescent solution.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/1797/version/1

Exploiting Nonlinear Recurrence and Fractal Scaling Properties for Voice Disorder Detection
Provedor de dados: 17
Autores: Max A. Little; Patrick E. McSharry; Stephen A. Roberts; Declan A. E. Costello; Irene  M. Moroz.
Background: Voice disorders affect patients profoundly, and acoustic tools can potentially measure voice function objectively. Disordered sustained vowels exhibit wide-ranging phenomena, from nearly periodic to highly complex, aperiodic vibrations, and increased &amp;#x22;breathiness&amp;#x22;. Modelling and surrogate data studies have shown significant nonlinear and non-Gaussian random properties in these sounds. Nonetheless, existing tools are limited to analysing voices displaying near periodicity, and do not account for this inherent biophysical nonlinearity and non-Gaussian randomness, often using linear signal processing methods insensitive to these properties. They do not directly measure the two main biophysical symptoms of disorder: complex...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/326/version/1

Exploring the biotechnologial applications in the archaeal domain
Provedor de dados: 58
Autores: Alquéres,S.M.C.; Almeida,R.V.; Clementino,M.M.; Vieira,R.P.; Almeida,W.I.; Cardoso,A.M.; Martins,O.B.
Archaea represent a considerable fraction of the prokaryotic world in marine and terrestrial ecosystems, indicating that organisms from this domain might have a large impact on global energy cycles. The extremophilic nature of many archaea has stimulated intense efforts to understand the physiological adaptations for living in extreme environments. Their unusual properties make them a potentially valuable resource in the development of novel biotechnological processes and industrial applications as new pharmaceuticals, cosmetics, nutritional supplements, molecular probes, enzymes, and fine chemicals. In the present mini-review, we show and discuss some exclusive characteristics of Archaea domain and the current knowledge about the biotechnological uses of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Archaea; Biotechnology; Extremozymes; Genomic; Molecular Phylogeny.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300002

Exploring The Hidden Realms Of Global Biodiversity: Interesting Endophytic Yeast Flora From Floral Ovaries
Provedor de dados: 17
Autores: Nandkumar Kamat; Shweta S. Saudagar.
Soil is considered the ultimate reservoir of all the microbiota  and specifically the yeasts. However their association with different plant organs and at different developmental stages is not fully explored. Although about 1000 yeast species have been identified, exploration of various microhabitats such as plant storage organs, flowers, nectar, and tree sap is expected to yield a large number of novel species, including interesting endophytic yeasts(Carlos &amp;#x26; Gabor, 2006). Angiosperms are considered the most diverse group of the plant kingdom comprising of about 250,000 species (Kenrick, 1999). As part of an exhaustive exploration of yeasts associated with angiosperm flowers, specific attention was paid to floral ovaries to detect the presence of...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Ecology; Genetics &amp; Genomics; Microbiology; Plant Biology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6268/version/1

Expression of bacterial genes in transgenic tobacco: methods, applications and future prospects
Provedor de dados: 69
Autores: Jube,Sandro; Borthakur,Dulal.
Tobacco is the most commonly used plant for expression of transgenes from a variety of organisms, because it is easily grown and transformed, it provides abundant amounts of fresh tissue and has a well-established cell culture system. Many bacterial proteins involved in the synthesis of commercial products are currently engineered for production in tobacco. Bacterial enzymes synthesized in tobacco can enhance protection against abiotic stresses and diseases, and provide a system to test applied strategies such as phytoremediation. Examples of bacterial gene expression in tobacco include production of antigen proteins from several human bacterial pathogens as vaccines, bacterial proteins for enhancing resistance against insects, pathogens and herbicides,...
Tipo: Journal article
Palavras-chave: Biotechnology; Environmental stress; Insect/pathogen/herbicide resistance; Phytoremediation; Transgenics; Vaccine production.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300013

Expression of Green Fluorescence Protein (GFP) in Zebrafish Muscle through Injection: A Gene Therapy Model
Provedor de dados: 17
Autores: C. S.  Lin; Jhen Ru  Shih; C. H.  Hsu; Z.H.  Wen; Chiranjib  Chakraborty.
Expression of the target gene is important for gene therapy. Presently, localized transgenesis is used for gene therapy which can be achieved by a target gene expression. Here, we have reported the plasmid mediated gene therapy to zebrafish model. For this purpose, we have chosen green fluorescent protein (GFP) as a target gene because the expression can be detected easily.  GFP was inserted in a plasmid vector, pQE30 to develop the vector pQE30GFP.  The plasmid pQE30GFP was constructed form plasmid, pQE30 and pEGFPC2. pQE30GFP injected directly in one group of fish into the muscle where luciferase expression was noted. In another group, after injection electroporation was performed where we have also noted luciferase expression; but, electroporation cause...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2718/version/1

Expression profile of genes involved in hydrogen sulphide liberation by _Saccharomyces cerevisiae_ grown under different nitrogen concentrations
Provedor de dados: 17
Autores: Ana  Mendes-Ferreira; Catarina Barbosa; Marcel.l&amp;#xed; del Olmo; Arlete Mendes-Faia; Cec&amp;#xed;lia Le&amp;#xe3;o.
The present work aims to elucidate molecular mechanisms underlying hydrogen sulphide production in _S. cerevisiae_ associated to nitrogen deficiency. To assess, at a genome-wide level, how the yeast strain adapted to the progressive nitrogen depletion and to nitrogen re-feeding, gene expression profiles were evaluated during fermentation at different nitrogen concentrations, using the DNA array technology. The results showed that most MET genes displayed higher expression values at the beginning of both control and N-limiting fermentation, just before the time at which the release of sulphide was observed. MET genes were downregulated when yeast stopped growing which could associate MET gene expression levels with cell growth. The over expression of MET...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/2736/version/1

Extração de DNA e obtenção de marcadores moleculares para diferentes espécies de interesse para o cerrado.
Provedor de dados: 14
Autores: BELLON, G.; FALEIRO, F. G.; BARROS, A. M.; KARIA, C. T.; ANDRADE, R. P.; CORDEIRO, M. C. R.; PINTO, A. C.; JUNQUEIRA, N. T. V.; PEREIRA, A. V.; PEREIRA, E. B. C.; FERNANDES, F. D.; FERREIRA, M. E.
bitstream/CPAC-2009/26863/1/p2005_54.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Planta nativa; Indigenous organisms.; Biotecnologia; Biologia Molecular; Cerrado; DNA; Marcador Genético; Marcador Molecular.; Genetic markers; Biotechnology; Molecular biology.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/553057

Extracting Functional Modules from Biological Pathways
Provedor de dados: 17
Autores: Sihai D. Zhao; Yong Li.
It has been proposed that functional modules are the fundamental units of cellular function. Methods to identify these modules have thus far relied on gene expression data or protein-protein interaction (PPI) data, but have a few limitations. We propose a new method, using biological pathway data to identify functional modules, that can potentially overcome these limitations. We also construct a network of these modules using functionally relevant PPI data. This network displays the flow and integration of information between modules and can be used to map cellular function.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1457/version/1

Facile Synthesis and Versatilities of Polyanthraquinoylamine Nanofibril Bundles with Self Stability and High Carbon Yield
Provedor de dados: 17
Autores: Xin-Gui Li; Mei-Rong Huang; Hu Li; Mark G. Moloney.
A facile synthesis for nanosized conducting polymers with inherent self-stability and multi-functionalities is a main challenge. Here we simply synthesize intrinsically self-stabilized nanofibril bundles of poly(1-anthraquinoylamine) (PAQ) by a template-free method. The critical polymerization parameters were studied to significantly optimize the synthesis, size, properties, and functionalities of the resulted fine nanofibrils with a diameter of ca. 30 nm and length of ~6 &amp;#x3bc;m. The PAQ obtained with ammonium persulfate possesses higher polymerization yield, purer composition, higher conductivity, better melting behaviour, higher thermostability, lower burning enthalpy, and slower degradation than that with other oxidants. Furthermore, the polymer...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/2396/version/1

Factors Affecting Biotechnology Innovation in Canada: Analysis of the 2001 Biotechnology Use and Development Survey
Provedor de dados: 31
Autores: van Moorsel,   Daryl; Cranfield,   John A.L.; Sparling,   David.
Advancement in biotechnology requires continued innovative activity by firms.  To grow, biotechnology firms must understand the factors affecting their innovative activity.  Such understanding also informs policy makers, and supports the development of policies promoting one's biotechnology sector.  This study explores factors which determine innovative activity within the Canadian biotechnology industry.  Innovative activity is measured as the natural log of the number of products/processes a firm has at different stages of the innovation spectrum.  A model is developed to regress this measure on several determinants of innovation.  Significant drivers of innovation include: collaborative arrangements, transfer of intellectual property, firm size and age,...
Tipo: Working or Discussion Paper
Palavras-chave: Innovation; Biotechnology; Canada; Agriculture; Food; Human health; Research and Development/Tech Change/Emerging Technologies.
Ano: 2005
URL: http://purl.umn.edu/34121

Failure of Abeta(1-40) amyloid fibrils under tensile loading
Provedor de dados: 17
Autores: Raffaella Paparcone; Markus J. Buehler.
Amyloid fibrils and plaques are detected in the brain tissue of patients affected by Alzheimer&amp;#x2019;s disease, but have also been found as part of normal physiological processes such as bacterial adhesion. Due to their highly organized structures, amyloid proteins have also been used for the development of novel nanomaterials, for a variety of applications including biomaterials for tissue engineering, nanolectronics, or optical devices. Past research on amyloid fibrils resulted in advances in identifying their mechanical properties, revealing a remarkable stiffness. However, the failure mechanism under tensile loading has not been elucidated yet, despite its importance for the understanding of key mechanical properties of amyloid fibrils and plaques...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5174/version/1

Farmer Acceptance of Genetically Modified seeds in Germany: Results of a Cluster Analysis
Provedor de dados: 31
Autores: Gyau,   Amos; Voss,   Julian; Spiller,   Achim; Enneking,   Ulrich.
www.ifama.org
Tipo: Journal Article
Palavras-chave: Biotechnology; Cluster analysis; German farmers; Agricultural and Food Policy; Crop Production/Industries; Farm Management; Food Security and Poverty; Q2.
Ano: 2009
URL: http://purl.umn.edu/92552

Fatty acids composition of some freshwater fish
Provedor de dados: 17
Autores: Chukwuemeka  Ugoala; George Ndukwe; Tokunbo Audu.
The aims of this paper were to investigate the component fatty acids of some freshwater fish species by comparing the levels of essential fatty acids present. The FAME were determined by GC-MS and were identified using retention time locked methods and retention time databases. The fatty acids profiles include minor amounts of odd-number, branched-chain, and even-number fatty acids as well as saturated components, the MUFA and PUFA. The major SFA were C14:0 and C16:0. The C18:1 was the prominent MUFA.  The dominant PUFA are of the omega-6 series and are found chiefly in C18:2 fatty acids. The essential fatty acids compositions showed prominence in C18:3n-3 and C18:2n-6. The branched chain fatty acids identified C15:0, C16:0, C17:0, C18:2 and C20:0. The...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/3239/version/1

FDserver: A web service for protein folding research
Provedor de dados: 17
Autores: Bin-Guang Ma; Ling-Ling Chen; Hong-Yu Zhang.
*Summary:* To facilitate the study of protein folding, we have developed a web service for protein folding rate and folding type prediction as well as for the calculation of a variety of topological parameters of protein structure, which is freely available to the community.&amp;#xd;&amp;#xa;*Availability:* http://sdbi.sdut.edu.cn/FDserver
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2349/version/1

Fingerprinting analysis of mango (Mangifera indica L.) cultivars introduced or developed in Brazil using RAPD markers.
Provedor de dados: 14
Autores: FALEIRO, F. G.; CORDEIRO, M. C. R.; PINTO, A. C. Q.; ROSSETTO, C. J.; BELLON, G.; ANDRSDE, S. R. M.; FRAGA, L. M. S.; SOUZA, T. L. P. O.
bitstream/CPAC-2009/24955/1/p2004_08.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: RAPD; Melhoramento genetico; Brasil; Mango.; Biotecnologia; Marcador Genético; Marcador Molecular.; Manga; Mangifera Indica; Brazil; Biotechnology; Genetic markers; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/566520

First reports of computed tomographic colonography for the screening of colorectal polyps in acromegalic patients
Provedor de dados: 17
Autores: Odery Ramos Jr; C&amp;#xe9;sar Luiz Boguszewski; Sandra Teixeira; Anke Boeving; Ricardo De Bem; Benito Parolim; J&amp;#xfa;lio C&amp;#xe9;sar Pisani; Jo&amp;#xe3;o Carlos Prolla.
*Aim:* To analyze the CTC performance for the screening of colorectal polyps in acromegalic patients.  &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;*Materials and Methods:* A prospective study of 21 acromegalic patients, 12 male and 9 female, average age 49, who underwent CTC and CC. CTC was performed with a GE Helical Multislice Computed Tomography Apparatus. The colonoscopy was performed, in the same day, without previous knowledge of the CTC diagnostics. The study evaluated the capacity of CTC to detect patients with colorectal polyps and identify each colorectal lesion described by CC.   &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;*Results:* In two patients (2/21), CC was incomplete. However, in all patients CTC was complete. In Phase I (&amp;#x201c;Per...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer.
Ano: 2008
URL: http://precedings.nature.com/documents/2323/version/1

Flaw-tolerance in silk fibrils explains strength, extensibility and toughness of spider silk
Provedor de dados: 17
Autores: Melis Arslan; Markus  J. Buehler.
Silk is an ancient but remarkably strong, extensible and tough material made from simple protein building blocks. Earlier work has shown that the particular molecular geometry of silk with a composite of semi-amorphous and nanocrystalline beta-sheet protein domains provides the structural basis for its characteristic softening-stiffening behavior and remarkable strength at the nanoscale. Yet, an open question remains as to how these nanoscale properties are upscaled so effectively to create strong, extensible and tough silk fibers. Here we discover that the geometric confinement of fibrils to &amp;#x2248;50-100 nm width and arranged in bundles to form larger-scale silk fibers, is the key to explaining the upscaling of the mechanical properties of silk from...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics; Earth &amp; Environment.
Ano: 2011
URL: http://precedings.nature.com/documents/5916/version/1

Fluorescence Correlation Spectroscopy as a Quantitative Tool Applied to Drug Delivery Model Systems
Provedor de dados: 17
Autores: Christoph Jung; Sigrun Polier; Markus Schoeffel; M Drechsler; Valerie Jerome; Ruth Freitag.
The delivery of drugs to cells is a very active area of research. Drugs are commonly believed to ameliorate illnesses. The targeted, controlled and/or enhanced uptake of the drug into cells is facilitated by loading the drug into carrier vehicles (nanoparticles, e.g.. micelles).The distribution of a drug over the nanoparticles used to deliver the drug to the targeted cells is of vital importance. It makes a difference, whether all nanoparticles carry the same amount of drug or whether a little amount of the nanoparticles carries a high proportion of the drug material and most nanoparticles carry no material. This depends on the method used to load the drugs into the carriers. Furthermore, the absolute amount of drug present in the nanoparticles and the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4140/version/1

Food Physical Chemistry and Biophysical Chemistry
Provedor de dados: 17
Autores: I.C. Baianu.
Food Physical Chemistry is considered to be a branch of Food Chemistry^1,2^ concerned with the study of both physical and chemical interactions in foods in terms of physical and chemical principles applied to food systems, as well as the applications of physical/chemical techniques and instrumentation for the study of foods^3,4,5,6^.  This field encompasses the &amp;#x22;physiochemical principles of the reactions and conversions that occur during the manufacture, handling, and storage of foods&amp;#x22;^7^. Two rapidly growing, related areas are Food Biotechnology and Food Biophysical Chemistry.  &amp;#xd;&amp;#xa;
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Microbiology; Molecular Cell Biology; Pharmacology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6573/version/1

Food Physical Chemistry and Biophysical Chemistry (v2)
Provedor de dados: 17
Autores: I. C. Baianu.
Food Physical Chemistry is considered to be a branch of Food Chemistry^1,2^ concerned with the study of both physical and chemical interactions in foods in terms of physical and chemical principles applied to food systems, as well as the applications of physical/chemical techniques and instrumentation for the study of foods^3,4,5,6^.  This field encompasses the &amp;#x22;physiochemical principles of the reactions and conversions that occur during the manufacture, handling, and storage of foods&amp;#x22;^7^. Two rapidly growing, related areas are Food Biotechnology and Food Biophysical Chemistry.  &amp;#xd;&amp;#xa;
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Microbiology; Molecular Cell Biology; Pharmacology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6573/version/2

Fractal-like Distributions over the Rational Numbers in High-throughput Biological and Clinical Data
Provedor de dados: 17
Autores: Vladimir Trifonov; Laura Pasqualucci; Riccardo Dalla-Favera; Raul Rabadan.
Recent developments in extracting and processing biological and clinical data are allowing quantitative approaches to studying living systems. High-throughput sequencing, expression profiles, proteomics, and electronic health records are some examples of such technologies. Extracting meaningful information from those technologies requires careful analysis of the large volumes of data they produce. In this note, we present a set of distributions that commonly appear in the analysis of such data. These distributions present some interesting features: they are discontinuous in the rational numbers, but continuous in the irrational numbers, and possess a certain self-similar (fractal-like) structure. The first set of examples which we present here are drawn...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5037/version/1

Fracture Toughness of Human Solid Cancers
Provedor de dados: 17
Autores: Nagesh Ragavendra; William Klug; J Woody  Ju.
Human solid cancers typically are harder and firmer than surrounding normal tissue upon clinical palpation. This characteristic has been linked to the presence of abundant collagen in the tumor stroma, referred to as the desmoplastic reaction. Recent evidence indicates that tumor-associated desmoplastic stroma is critical to cancer&amp;#x2019;s growth and progression. However, how best to measure, interpret, and/or model the mechanical attributes of tumor microenvironment in vivo remain unclear. &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;During routine sonographically-guided fine needle biopsy of solid tumors of the human thyroid gland, it was serendipitously observed by the lead author that the nature and strength of the haptic force feedback cues varied among...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer.
Ano: 2008
URL: http://precedings.nature.com/documents/2461/version/1

From aptamer-based biomarker discovery to  diagnostic and clinical applications: an aptamer-based, streamlined multiplex proteomic assay
Provedor de dados: 17
Autores: Stephan Kraemer; Jonathon  D. Vaught; Christopher Bock; Larry Gold; Evaldas Katilius; Tracy R. Keeney; Nancy Kim; Nicholas A. Saccomano; Sheri K. Wilcox; Dom Zichi; Glenn M. Sanders.
Recently, we reported an aptamer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science applications, we developed a streamlined, plate-based version of the assay. The plate-based version of the assay is robust, sensitive (sub-picomolar), rapid, can be highly multiplexed (upwards of 60 analytes), and fully automated.  We demonstrate that quantification by microarray-based hybridization, Luminex bead-based methods, and qPCR are each compatible with our platform, further expanding the breadth of proteomic applications for a wide user community.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4642/version/1

Future of Agriculture in the Semi-Arid Tropics
Provedor de dados: 30
Autores: M.C.S.
Palavras-chave: Water harvesting; Land resources; Tropical zones; Policies; Products; Biotechnology; Soil degradation; Sustainable agriculture; Electrophoresis; Agricultural trade.
Ano: 2001
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3696

General updates about SBML and SBML Team activities
Provedor de dados: 17
Autores: Michael Hucka.
In this presentation, I summarize very briefly some recent updates to SBML.org, SBML development, and software from the SBML Team.
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6345/version/1

Generalized Theory of Life
Provedor de dados: 17
Autores: Vyacheslav L. Kalmykov.
The paper is mathematically, physically and biologically inspired - it is a deductive attempt of the biologist to find the most appropriate language for modelling of life. Applications of mathematical structures - groupoids, groups, categories, functors and monads, which are the most effective for the description of the complex objects, are discussed. Some new conceptions and interpretations were introduced: unified classification of complex systems; the generalized conception about stationary states of any autonomous agent (&amp;#x22;Statology&amp;#x22;); the total quantitative criterions of evolutionary direction; the generalized group of symmetry. The definitions of information, entropy, organization, behavior, evolution, creation, life, culture,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Bioinformatics; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7108/version/1

Generation of &amp;#x22;backbone&amp;#x22; free, low transgene copy plants by launching T-DNA from the _Agrobacterium_ chromosome
Provedor de dados: 17
Autores: Heiko Oltmanns; Bronwyn Frame; Lan-Ying Lee; Susan Johnson; Bo Li; Kan Wang; Stanton Gelvin.
In both applied and basic research, _Agrobacterium_-mediated transformation is commonly used to introduce genes into plants. We investigated the effect of three _Agrobacterium tumefaciens_ strains and five T-DNA origins of replication on transformation frequency, transgene copy number, and the frequency of integration of non-T-DNA portions of the T-DNA-containing vector (&amp;#x22;backbone&amp;#x22;) into the genome of _Arabidopsis thaliana_ and _Zea mays_. Launching T-DNA from the _picA_ locus of the _Agrobacterium_ chromosome increases the frequency of single transgene integration events and almost eliminates the presence of vector backbone sequences in transgenic plants. Along with novel _Agrobacterium_ strains we have developed, our findings are useful...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3497/version/1

Generation of Human Epidermis-Derived Mesenchymal Stem Cell-like Pluripotent Cells and their reprogramming in mouse chimeras
Provedor de dados: 17
Autores: Bing Huang; KaiJing Li; Jie Yu; Min Zhang; YongPing Li; XiaoPing Xu; Ting Luo; JianFa Huang; LiPing Guan; WeiHua Li; WenXin Zhang; ShaoChun Lin; XinTao Huang; LiPing Lin; YongLiang Lin; YiChi Zhang; WenCong Wang; ZhiChong Wang; QianYing Gao; XiGu Chen; XinMing Song; Jian Ge.
Stem cells can be derived from the embryo (embryonic stem cells, ESCs), from adult tissues (adult stem cells, ASCs), and by induction of fibroblasts (induced pluripotent stem cells, iPSs). Ethical problems, immunological rejection, and difficulties in obtaining human tissues limit the use of ESCs in clinical medicine. Induced pluripotent stem cells are difficult to maintain in vitro and carry a greater risk of tumor formation. Furthermore, the complexity of maintenance and propagation is especially difficult in the clinic. Adult stem cells can be isolated from several adult tissues and present the possibility of self-transplantation for the clinical treatment of a variety of human diseases. Recently, several ASCs have been successfully isolated and...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6016/version/1

Genes are gems:reporting agri-biotechnology
Provedor de dados: 30
Autores: R.L.
Palavras-chave: Biotechnology; Transgenics; Genes; DNA; Mycotoxins; Chickpeas; Transgenic plants; Genetic markers; Genetic engineering; Genomes.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4146

Genes2Networks: Connecting Lists of Proteins by Using Background Literature-based Mammalian Networks
Provedor de dados: 17
Autores: Avi Ma&amp;#x27;ayan.
In recent years, in-silico literature-based mammalian protein-protein interaction network datasets have been developed. These datasets contain binary interactions extracted manually from legacy experimental biomedical research literature. Placing lists of genes or proteins identified as significantly changing in multivariate experiments, in the context of background knowledge about binary interactions, can be used to place these genes or proteins in the context of pathways and protein complexes.&amp;#xd;&amp;#xa;Genes2Networks is a software system that integrates the content of ten mammalian literature-based interaction network datasets. Filtering to prune low-confidence interactions was implemented. Genes2Networks is delivered as a web-based service using...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/35/version/1

Genes2Networks: Connecting Lists of Proteins by Using Background Literature-based Mammalian Networks
Provedor de dados: 17
Autores: Jeremy I. Posner; Seth Berger; Avi M. Ma&amp;#x27;ayan.
In recent years, in-silico literature-based mammalian protein-protein interaction network datasets have been developed. These datasets contain binary interactions extracted manually from legacy experimental biomedical research literature. Placing lists of genes or proteins identified as significantly changing in multivariate experiments, in the context of background knowledge about binary interactions, can be used to place these genes or proteins in the context of pathways and protein complexes.&amp;#xd;&amp;#xa;Genes2Networks is a software system that integrates the content of ten mammalian literature-based interaction network datasets. Filtering to prune low-confidence interactions was implemented. Genes2Networks is delivered as a web-based service using...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/35/version/2

Genetic Analysis of ABO and Rh Blood Groups in Brahmin Population of Uttar Pradesh, India&amp;#x201d;
Provedor de dados: 17
Autores: Vandana V. Rai.
A series of glycoprotein and glycolipids on the red blood cell surface constitute blood group antigens. These are A, B, AB and O in ABO blood group system and Rh in rhesus blood group system. These antigens are genetically controlled. Certain diseases have been shown to be associated with certain blood groups. In the present study an attempt is made to study the distribution of ABO and Rh. (D) blood group systems among the Brahmin caste population of Uttar Pradesh State. A total of 200 unrelated Brahmin individuals from Uttar Pradesh were studied for the phenotype and allele frequency distribution of ABO and Rh (D) blood groups. The order of occurrence of ABO phenotypes is B&amp;#x3e;O&amp;#x3e;A&amp;#x3e;AB. The corresponding allele frequencies of O, A,...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/5931/version/1

Genetic and Immune Predictors for Hypersensitivity Syndrome to Antiepileptic Drugs
Provedor de dados: 17
Autores: Manuela G. Neuman; Radu M. Nanau; Lawrence Cohen; Paul A. Hwang.
Hypersensitivity syndrome reactions (HSR) to antiepileptic drugs (AED) are associated with severe clinical cutaneous adverse reactions (SCAR).Our aims are: to assess HSRs to AEDs using the in vitro lymphocyte toxicity assay (LTA) in patients who manifested HSRs clinically, to correlate LTA results with the clinical syndrome, to correlate LTA results with the human leukocyte antigen (HLA) allele B*1502 (HLA-B*1502) positivity in a Han Chinese-Canadian population, and to determine the cytokine network in this population. HSR patients developed fever and cutaneous eruptions in the presence or absence of organ involvement within 8 weeks of exposure to carbamazepine (CBZ), phenytoin (PHY) or lamotrigine (LTG). Control patients received AEDs without presenting...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Immunology; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/6477/version/1

Genetic engineering applications in animal breeding
Provedor de dados: 69
Autores: Montaldo,Hugo H.
This paper discusses the use of genetic engineering applications in animal breeding, including a description of the methods, their potential and current uses and ethical issues. Genetic engineering is the name of a group of techniques used to identify, replicate, modify and transfer the genetic material of cells, tissues or complete organisms. Important applications of genetic engineering in animal breeding are: 1) Marker-assisted selection (MAS). The objective of this technology is to increase disease resistance, productivity and product quality in economically important animals by adding information of DNA markers to phenotypes and genealogies for selection decisions. 2) Transgenesis, the direct transfer of specific genes/alleles between individuals,...
Tipo: Journal article
Palavras-chave: Adult mammalian cloning; Biotechnology; Gene mapping; GMOS; MAS; QTL; Transgenics.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200010

Genetic improvement of horticultural crops mediated by CRISPR/Cas: a new horizon of possibilities
Provedor de dados: 75
Autores: Koltun,Alessandra; Erpen-Dalla Corte,Lígia; Mertz-Henning,Liliane Marcia; Gonçalves,Leandro SA.
ABSTRACT The burden of the current global challenge involving food security lies in the need to improve crop production. In this regard, biotechnology stands out as an essential tool to generate plants able to cope with pests, diseases, and harsh climatic conditions, and more efficient in the use of natural resources. An advanced approach to create genetic variability in a precise and targeted way, the genome-editing technique CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins), has drawn the attention of breeders. The genome editing CRISPR/Cas system relies on a guiding RNA that directs a nuclease to generate a double-strand break (DSB) at a target DNA, activating the cell repair systems and eventually leading...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genome editing; Plant breeding; Biotechnology; Induced mutations; Non-transgenic.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000300290

Genetic incorporation of D-Lysine into diketoreductase in Escherichia coli cells
Provedor de dados: 17
Autores: Zhi Zhi Liu; Xin Yang; Denghuan Yi; Shuzhen Wang; Yijun Chen.
D-Lysine has been genetically introduced into diketoreductase in E. coli cells by utilization of an orthogonal Ph tRNA /Lysyl-tRNA synthetase pair. This is the first report on the genetic incoporation of D-amino acids into proteins, which may be generally applicable to a wide variety of applications.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Microbiology; Molecular Cell Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6777/version/1

Genetic Normalization of Differentiating Aneuploid Human Embryos
Provedor de dados: 17
Autores: Paul Brezina; Andrew Barker; Andrew Benner; Ric Ross; Khanh-Ha Nguyen; Raymond Anchan; Kevin Richter; Garry Cutting; William Kearns.
Early embryogenesis involves a series of dynamic processes, many of which are currently not well described or understood. Aneuploidy and aneuploid mosaicism, a mixture of aneuploid and euploid cells within one embryo, in early embryonic development are principal causes of developmental failure.^1,2^ Here we show that human embryos demonstrate a significant rate of genetic correction of aneuploidy, or &amp;#x22;genetic normalization&amp;#x22; when cultured from the cleavage stage on day 3 (Cleavage) to the blastocyst stage on day 5 (Blastocyst) using routine in vitro fertilization (IVF) laboratory conditions. One hundred and twenty-six human Cleavage stage embryos were evaluated for clinically indicated preimplantation genetic screening (PGS). Sixty-four of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6045/version/1

Genetic variability among _Coleus sp_ studied by RAPD banding pattern analysis
Provedor de dados: 17
Autores: Muthusamy Govarthanan; Arunapriya S; Guruchandar A; Selvankumar T; Gnanasekaran N; Manoharan Koildhasan.
Genetic improvement of the medicinal plants depends upon the existence, nature and extent of the genetic variability available for manipulation. Genetic analysis with RAPD markers has been extensively used to determine genetic diversity among _Coleus sp_ and to identify the best quality for human consumption for its medicinal purpose. The objectives of the present study were to assess molecular variation among _C.amboinicus_, _C.aromaticus_ and _C.forskohlii_. and to determine the level of genetic similarity among them. We performed random amplification of polymorphic DNA (RAPD) analysis on three strains of _Coleus sp_. Random primers were used for the PCR. Electrophoresis on denaturing acrylamide gels improved RAPD reproducibility and increased the band...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/5631/version/1

Genetic variability of mango (Mangifera indica L.) cultivars used in the Embrapa Cerrados breeding program using RAPD markers.
Provedor de dados: 14
Autores: FALEIRO, F. G.; PINTO, A. C. Q.; CORDEIRO, M. C. R.; RAMOS, V. H. V.; BELLON, G.; ANDRADE, S. R. M.; PINTO, J. F. N.
bitstream/CPAC-2009/24954/1/p2004_07.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Melhoramento genetico; RAPD; Mango.; Biotecnologia; Cerrado; Marcador Molecular; Manga; Mangifera Indica; Marcador Genético; Método de Melhoramento; Variação Genética.; Genetic variation; Breeding methods; Genetic markers; Biotechnology; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/566512

Genética e biotecnologia aplicada ao melhoramento genético vegetal: relatório de pós-doutorado.
Provedor de dados: 14
Autores: FALEIRO, F. G.
bitstream/item/84605/1/doc-309.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Biotecnologia; Genética; Melhoramento Vegetal.; Biotechnology; Genetics; Plant breeding.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/958925

GENETICALLY ENGINEERED CROPS FOR PEST MANAGEMENT IN U.S. AGRICULTURE
Provedor de dados: 31
Autores: Fernandez-Cornejo,   Jorge; McBride,   William D.
Adoption of genetically engineered crops with traits for pest management has risen dramatically since their commercial introduction in the mid-1990's.  The farm-level impacts of such crops on pesticide use, yields, and net returns vary with the crop and technology examined.  Adoption of herbicide-tolerant cotton led to significant increase in yields and net returns, but was not associated with significant changes in herbicide use.  On the other hand, increase in adoption of herbicide-tolerant soybeans led to small but significant increases in yields, no changes in net returns, and significant decreases in herbicide use.  Adoption of Bt cotton in the Southeast significantly increased yields and net returns and significantly reduced insecticide use.
Tipo: Report
Palavras-chave: Biotechnology; Genetic engineering; Pest management; Field crops; Input traits; Crop Production/Industries.
Ano: 2000
URL: http://purl.umn.edu/33931

Genetically modified Cotton species detection by LISS-III satellite data
Provedor de dados: 17
Autores: Saumitra Mukherjee; Satyanarayan  Shashtri; Chander  Kumar Singh; Bindu  Kumari; Reena  Kumari; Ram  Avatar; Anita  Mukherjee; Bhoop  Singh.
It is possible to infer the genetically modified species by using remotely sensed data. Using ERDAS software the algorithm of BT (Bacillus thuringiensis) Cotton in Punjab, India was developed successfully. GPS enabled space technology has the potential to identify the exact location of Bt Cotton by generating Normalized Difference Vegetation Index (NDVI) for the calculation of total area covered by this species. It was possible to develop a correlation in between genetically modified Cotton crop and NDVI value. In parts of Bhatinda district of Punjab the yield of Bt Cotton and NDVI showing R2 value of more than 4.5 in regression analysis. A correlation matrix was also generated which shows that NDVI values of BT cotton has reasonably acceptable correlation...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Earth &amp; Environment.
Ano: 2008
URL: http://precedings.nature.com/documents/2659/version/1

GENETICALLY MODIFIED CROP INNOVATIONS AND PRODUCT DIFFERENTIATION: TRADE AND WELFARE EFFECTS IN THE SOYBEAN COMPLEX
Provedor de dados: 31
Autores: Sobolevsky,   Andrei; Moschini,   GianCarlo; Lapan,   Harvey E.
We develop a new partial equilibrium, four-region world trade model for the soybean complex comprising soybeans, soybean oil, and soybean meal. In the model, some consumers view genetically modified Roundup Ready (RR) soybeans and products as weakly inferior to conventional ones; the RR seed is patented and sold worldwide by a U.S. firm; and producers employ a costly segregation technology to separate conventional and biotech products in the supply chain. The calibrated model is solved for equilibrium prices, quantities, production patterns, trade flows, and welfare changes under different assumptions regarding regional government's production and trade policies, differentiated consumer tastes, and several other demand and supply parameters. Incomplete...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Differentiated demand; Food labeling; Genetically modified products; Identity preservation; Innovations; Intellectual property rights; International trade; Loan deficiency payments; Market failure; Monopoly; Roundup Ready soybeans; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2002
URL: http://purl.umn.edu/18348

Genetically Modified Crops and the Agroecosystem: Comments on "Genetically Modified Crops: Risks and Promise" by Gordon Conway
Provedor de dados: 7
Autores: Pimentel, David; Cornell University; dp18@cornell.edu.
Tipo: Peer-Reviewed Reports
Palavras-chave: Bacillus thuringiensis (B.t.); GM crops; Agriculture; Biotechnology; Economics; Ecosystems; Genetic engineering; Herbicides.
Ano: 2000

Genome Replikin Count Predicts Increased Infectivity/Lethality of Viruses
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
The genomes of all groups of viruses whose sequences are listed on Pubmed, specimens since 1918, analyzed by a software from Bioradar UK Ltd., contain Replikins which range in concentration from a Replikin Count (number of Replikins per 100 amino acids) of less than 1 to 30 (see accompanying communications for higher Counts in tuberculosis, malaria, and cancer, associated with higher lethality). Counts of less than 4.0 were found in &amp;#x2018;resting&amp;#x2019; virus states; Counts greater than 4.0, found to be associated with rapid replication, were found invariably to accompany or to predict virus outbreaks, by as much as two years, in viral hosts examined from salmon, to birds, to livestock, to humans. X-ray diffraction showed Replikins to be on the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7144/version/1

Genome Replikin Count Predicts Increased Lethality of Cancer
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Those cancers, like thyroid and pancreatic cancer, with the lowest 5-year mortality rates, have Replikin Counts of about 20; lung and brain cancers, with the highest 5-year mortality rates, have Replikin Counts of 275 and 325 respectively; the others fall in between with approximate linearity. This is the first quantitative relationship of a genomic structure, Replikins, to 5-year mortality rates.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics &amp; Genomics; Immunology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7143/version/1

Genome Replikin Count Predicts Increased Lethality of Resistant Tuberculosis
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
The genomic Replikin Count of all the sequences  on Pubmed of different strains of tuberculosis were analyzed. The lowest Counts occurred with species within the lowest drug resistance, the highest Counts with sequences of the highest drug resistance and lethality.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7141/version/1

Genomic Replikin Count Predicts Increased Lethality of Malaria
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Genomic Replikin Counts predict both the increase and the decrease of lethality of malaria
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Immunology; Microbiology; Molecular Cell Biology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7142/version/1

Genomic Replikin Counts of Infectious Salmon Anemia Virus (ISAV) in Canada Exceed the Counts in Lethal Outbreaks in Norway, Chile, and Scotland. Real-Time Tracking of the Evolution of the ISAV Genome and the Resultant Replikins Solid Phase ISAV Vaccine Make ISAV Pandemic Prevention Possible.
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Genomic Replikin CountsTM  of Infectious Salmon Anemia Virus (ISAV) in Canada Exceed the Counts in Lethal Outbreaks in Norway, Chile, and Scotland. Real-Time Tracking of the Evolution of the ISAV Genome and the Resultant Replikins Solid Phase ISAV Vaccine Make ISAV Pandemic Prevention Possible.&amp;#xd;&amp;#xa;
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6991/version/1

Genomics and synthetic biology as a viable option to intensify sustainable use of biodiversity
Provedor de dados: 17
Autores: Elibio L. Rech.
The Amazon basin is an area of mega-biodiversity. Different models have been proposed^1-8^ for the establishment of an effective conservation policy, increasing sustainability and adding value for biodiversity. Currently, a broad spectrum of technologies from genomics to synthetic biology is available, and these permit the collection, manipulation and effective evaluation of countless organisms, metabolic pathways and molecules that exist as potential products of a large, biodiverse ecosystem. The use of Genomics and synthetic biology may constitute an important tool and be a viable option for the prospection, evaluation and manipulation of biodiversity as advocated as well as be useful for developing methods for sustainable use and the production of novel...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Earth &amp; Environment.
Ano: 2011
URL: http://precedings.nature.com/documents/5759/version/1

Geometric bionics: Lotus effect helps polystyrene nanotube films get good blood compatibility
Provedor de dados: 17
Autores: Chun Mao; Wen-Bo Zhao; Wen-Ping Luo; Cun-Xia Liang; Xiao-Mei Hou; Xiao-Hua Huang; Hong-Ke Liu; Ying-Hong Xiao; Jian-Chun Bao; Jian Shen.
Various biomaterials have been widely used for manufacturing biomedical applications including artificial organs, medical devices and disposable clinical apparatus, such as vascular prostheses, blood pumps, artificial kidney, artificial hearts, dialyzers and plasma separators, which could be used in contact with blood^1^. However, the research tasks of improving hemocompatibility of biomaterials have been carrying out with the development of biomedical requirements^2^. Since the interactions that lead to surface-induced thrombosis occurring at the blood-biomaterial interface become a reason of familiar current complications with grafts therapy, improvement of the blood compatibility of artificial polymer surfaces is, therefore a major issue in biomaterials...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/3002/version/1

Germinating the seeds of success in the semi-arid tropics
Provedor de dados: 30
Autores: William D.
Palavras-chave: Pigeonpeas; Sorghum; Millets; Chickpeas; Transgenics; Biotechnology; Electrophoresis; Groundnuts; Agroecosystems; Genetic transformation.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4145

Getting The Most Out of Your Research
Provedor de dados: 17
Autores: Folarin A. Erogbogbo.
Research is the cornerstone of invention, facilitator of innovation, and mother of discovery. Even though many students are competent in advancing through the class room and getting good grades, excellent research requires a different set of attitudinal and academic skills. This workshop, &amp;#x22;Get the most out of your research&amp;#x22; is an outstanding program developed from extensive time spent with brilliant scientists and a wide variety of mentored students.  Familiarize yourself with the fine points of this workshop and arm yourself for a successful research career.
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2011
URL: http://precedings.nature.com/documents/5702/version/1

Glutathione (GSH) conjugates with dopamine (DA)-derived quinones to form reactive or non-reactive GSH-conjugates
Provedor de dados: 17
Autores: Zhidong Zhou; Tit Meng Lim.
In this study we demonstrate for the first time that GSH could rapidly conjugate with dopamine (DA)-derived DA-o-quinones without enzymatic catalysis to form short-lived intermediate GSH-conjugates (2-S-GSH-DA-o-quinone and 5-S-GSH-DA-o-quinone). These intermediate GSH-conjugates are unstable and would finally form reactive or non-reactive GSH-conjugates dependent on ambient reductive forces. Under insufficient reductive forces, the intermediate GSH-conjugates could cyclize spontaneously to form reactive 7-S-GSH-aminochrome (7-S-GSH-AM). The 7-S-GSH-AM is so reactive that it could further react with another GSH to form 4,7-bi-GSH-5,6-dihydroindole. Its reactivity could also abrogate tyrosinase activity in solutions. In addition, the 7-S-GSH-AM could...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Pharmacology.
Ano: 2009
URL: http://precedings.nature.com/documents/2963/version/1

GM crop technology and trade restraints: economic implications for Australia and New Zealand
Provedor de dados: 31
Autores: Anderson,   Kym; Jackson,   Lee Ann.
How much might the potential economic benefit from enhanced farm productivity associated with crop biotechnology adoption by Australia and New Zealand (ANZ) be offset by a loss of market access abroad for crops that may contain genetically modified (GM) organisms? This paper uses the Global Trade Analysis Project (GTAP) model to estimate effects of other countries’ GM policies without and with ANZ farmers adopting GM varieties of various grains and oilseeds. The gross economic benefits to ANZ from adopting GM crops under a variety of scenarios could be positive even if the strict controls on imports from GM-adopting countries by the European Union are maintained, but not if North-East Asia also applied such trade restaints. From those gross economic...
Tipo: Article
Palavras-chave: Biotechnology; Computable general equilibrium; Genetically modified organisms; Regulation; Trade policy; Crop Production/Industries; International Relations/Trade.
Ano: 2005
URL: http://purl.umn.edu/118502

GM food technology abroad and its implications for Australia and New Zealand
Provedor de dados: 31
Autores: Anderson,   Kym; Jackson,   Lee Ann.
The potential economic benefits from agricultural biotechnology adoption by ANZ need to be weighed against any likely loss of market access abroad for crops that may contain genetically modified (GM) organisms. This paper uses the global GTAP model to estimate effects of other countries' GM policies without and with ANZ farmers adopting GM varieties of various grains and oilseeds. The benefits to ANZ from adopting GM crops under a variety of scenarios are positive even in the presence of the ban on imports from GM-adopting countries by the EU (but not if East Asia also applied such a ban).
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; GMOs; Regulation; Trade policy; Computable general equilibrium; Crop Production/Industries; Food Consumption/Nutrition/Food Safety; C68; D58; F13; O3; Q17; Q18.
Ano: 2004
URL: http://purl.umn.edu/58365

GO faster ChEBI with Reasonable Biochemistry
Provedor de dados: 17
Autores: Duncan Hull.
Chemical Entities of Biological Interest (ChEBI) is a database and ontology that represents biochemical knowledge about small molecules. Recent changes to the ontology have created new opportunities for automated reasoning with description logic, that have not previously been fully exploited in Chemistry. These changes open up the possibility of building an improved chemical semantic web, by making more use of necessary and sufficient conditions, allowing reasoning about chemical structure, highlighting ambiguous inconsistencies and improving alignment with the Gene Ontology (GO). This paper briefly discusses some of the problems with reasoning over the current version of ChEBI, to tackle these issues, and their potential solutions.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2329/version/1

Green Fluorescent Protein (GFP) in Vector Systems Played Sense Role of Epigenetic in Plants
Provedor de dados: 17
Autores: Hany A. El-Shemy; Mutasim M. Khalafalla; Masao Ishimoto.
The green fluorescent protein (GFP) of jellyfish (_Aequorea victoria_) has significant advantages over other reporter genes, because expression can be detected in living cells without any substrates. Recently, epigenetic phenomena are important to consider in plant biotechnology experiments for elucidate unknown mechanism. Therefore, soybean immature cotyledons were generated embryogenesis cells and engineered with two different gene constructs (pHV and pHVS) using gene gun method. Both constructs contain a gene conferring resistance to hygromycin (_hpt_) as a selective marker and a modified glycinin (11S globulin) gene (_V3-1_) as a target. However, sGFP(_S65T_) as a reporter gene was used only in pHVS as a reporter gene for study the relation between...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Plant Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1845/version/1

Guided Tissue Remineralization of Resin-Bonded Acid-Etched Dentin
Provedor de dados: 17
Autores: Franklin R. Tay; David H. Pashley.
Contemporary biomineralization strategies incorporate non-classical crystallization pathways of fluidic amorphous nanoprecursors and mesoscopic transformation. Using two functional biomimetic molecules, we previously regenerated mineralized dentin from acid-etched dentin using the Guided Tissue Remineralization (GTR) approach, with definitive intrafibrillar remineralization of type-I collagen. Degradation of denuded collagen within dentin adhesive resin-infiltrated dentin is a pertinent problem in dentin bonding. Here, we show that GTR provides a means of salvaging these degrading bonds by remineralizing resin-dentin interfaces. The GTR medium consists of a Portland cement/simulated body fluid that includes polyacrylic acid and polyvinylphosphonic acid...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2022/version/1

H5N1 Clade 2.2 Polymorphism Tracing Identifies Influenza Recombination and Potential Vaccine Targets
Provedor de dados: 17
Autores: Henry L. Niman; Magdi D. Saad; Elassai M. Labib; E Ayoub; Mona M. Aly; A-SA Arafa; Marshall R. Monteville; Bruce R. Boynton; Jeffery Tjaden; Kenneth C. Earhart; Moustafa M. Mansour; Nasr M. ElSayed; A I. Nayei; A A. Abdelghani; Hala M. Essmat.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong Kong in 1997 1. The number of confirmed human cases now exceeds 300 and the associated Case Fatality Rate exceeds 60% 2. The genetic diversity of the serotype continues to increase.  Four distinct clades or sub-clades have been linked to human cases 3.4. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that lack an editing function, thereby resulting in antigenic drift 5. We traced polymorphism acquisition in Clade 2.2 sequences. We report here the concurrent acquisition of the same polymorphism by multiple, genetically distinct, Clade 2.2 sub-clades in Egypt, Russia...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/553/version/2

H5N1 Clade 2.2 Polymorphism Tracing Identifies Influenza Recombination and Potential Vaccine Targets
Provedor de dados: 17
Autores: Henry L. Niman; Magdi D. Saad; Bruce R. Boynton; Jeffery Tjaden; Kenneth C. Earhart; Moustafa M. Mansour; Nasr M. ElSayed; A I. Nayei; A A. Abdelghani; Hala M. Essmat; Elassai M. Labib; E Ayoub; Mona M. Aly; A-SA Arafa; Marshall R. Monteville.
Highly pathogenic Influenza A H5N1 was first identified in Guangdong Province in 1996, followed by human cases in Hong Kong in 1997 1. The number of confirmed human cases now exceeds 300 and the associated Case Fatality Rate exceeds 60% 2. The genetic diversity of the serotype continues to increase.  Four distinct clades or sub-clades have been linked to human cases 3.4. The gradual genetic changes identified in the sub-clades have been attributed to copy errors by viral encoded polymerases that lack an editing function, thereby resulting in antigenic drift 5. We traced polymorphism acquisition in Clade 2.2 sequences. We report here the concurrent acquisition of the same polymorphism by multiple, genetically distinct, Clade 2.2 sub-clades in Egypt, Russia...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/553/version/1

Haplodiploid androgenetic breeding in oat: genotypic variation in anther size and microspore development stage
Provedor de dados: 63
Autores: Cesaro,Taniela De; Baggio,Maria Irene; Zanetti,Silvia Andréia; Suzin,Marilei; Augustin,Lizete; Brammer,Sandra Patussi; Iorczeski,Edson Jair; Milach,Sandra Cristina Kothe.
Oat (Avena spp.) is poorly responsive to the haplodiploidization process, which leads to the production of homozygous lines in one step, increasing breeding efficiency. Androgenetic haploids in small grain cereal crops are obtained from microspores cultured at the mononucleate stage, which can be identified by the size of anthers. In order to identify the appropriate anther size for in vitro culture, microspore cytological analyses were made in Avena sativa cultivars UPF 7, UPF 18, UFRGS 14, Stout and Avena sterilis CAV 3361, cultivated in growth chamber under controlled light and temperature conditions. Variation was observed within and among genotypes for anther size at each microspore developmental stage and according to the position of spikelets in the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Androgenesis; Anther culture; Biotechnology; Haploid breeding; Microspores.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000100016

Harnessing Aquatic Yeasts For Developing A New Generation of Sunscreens
Provedor de dados: 17
Autores: Hycienth Braganca; Nandkumar Kamat.
Around 100 yeast genera and 800 species have been isolated and characterized. Nagahama (2006) has reviewed yeast biodiversity in freshwater, marine and deep-Sea Environments. Our work aims at systematic biodiversity assessment of freshwater aquatic yeasts from Goa with major focus on carotenogenic basidiomycetes yeasts which on exposure to potentially damaging levels of solar ultraviolet radiation (UVR) accumulate photoprotective compounds (PPC) such as mycosporine-glutaminol-glucoside (myc-glu-glu, peak absorption at 309-310nm) that server as sunscreens and /or antioxidant. These yeasts are a potential source of a particularly diverse family of such substances, collectively referred to as mycosporine- like amino-acids (MAAs). This paper presents the...
Tipo: Poster
Palavras-chave: Biotechnology; Ecology; Microbiology; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4249/version/1

Have Biotech Seeds Increased Maize Yields?
Provedor de dados: 31
Autores: Xu,   Zheng; Hennessy,   David A.; Moschini,   GianCarlo.
Corn yield is determined by soils, weather, seed used and other technology choices. Global population and per capita income growth trends as well as demand from the energy sector have placed great stress on cropland use. Global cropland acres and/or yield per acre will need to increase. Whether new seed technologies have enhanced corn yield is a controversial issue. We study U.S. county corn yields 1964-2008, controlling for location effects, fertilization technologies and weather. We find evidence that trend yield growth has been fastest in the Central Corn Belt, genetic modification technologies have increased trend yield, and this increase has been largest in the Central Corn Belt.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Corn Yield; Trend; Regional Effects; Weather; Fertilization.; Crop Production/Industries; Industrial Organization; Research and Development/Tech Change/Emerging Technologies; L65; Q16.
Ano: 2010
URL: http://purl.umn.edu/61303

HBsAg-vectored DNA vaccines elicit concomitant protective responses to multiple CTL epitopes relevant in human disease.
Provedor de dados: 17
Autores: Dekun Chen; Allan Gould; Michael Mather; Oscar Haigh; Melanie Barnes; Jacqueline Kattenbelt; Scott Thomson; Robert R. Tindle.
Vaccines capable of controlling neoplastic and infectious diseases which depend on the cellular immune response for their resolution, have proven difficult to develop. We, and others, have previously demonstrated that the potent immunogenicity of hepatitis B surface antigen (HBsAg), the already- licensed human vaccine for hepatitis B infection, may be exploited to deliver foreign antigens for cytotoxic T-lymphocyte (CTL) induction. In this study we demonstrate that recombinant (r) HBsAg DNA delivering a CTL polyepitope appended at the C&amp;#x27; terminus elicits concomitant responses to multiple epitopes restricted through a diversity of MHC class I haplotypes, which are relevant in a number of human diseases. We show that the rHBsAg DNA vaccine elicits...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Immunology.
Ano: 2008
URL: http://precedings.nature.com/documents/1910/version/1

Herbal Drugs in Mirror of Alzheimer&amp;#x2019;s Disease
Provedor de dados: 17
Autores: Bernd Kastenholz; Kerstin Nagel; David E. Garfin; J&amp;#xfc;rgen Horst.
Commercially available Ginkgo biloba leaf extracts may lack specificity as to the binding of copper and zinc ions in human cells. This might be the reason why Ginkgo extracts have no predictable and clinically significant benefit for people with dementia or cognitive impairment. A novel generation of herbal drugs is proposed as antioxidant and metal chelator in the treatment of Alzheimer&amp;#x27;s disease.
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience; Pharmacology; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3334/version/1

Heterogeneity in Ty1-copia group of retroelements in chickpea (Cicer arietinum) genome
Provedor de dados: 17
Autores: Manoj K. Rajput; Kailash C. Upadhyaya.
Retrotransposons constitute a major fraction of plant genomes and these elements may have played a significant role in evolution and sequence organization of genomes. In order to access the diversity of Ty1-copia group of retroelements, reverse transcriptase (RT) sequences were amplified from chickpea genome, using the primers derived from two conserved domains of RT region. Thirty-six RT regions from independent amplicons were cloned and sequenced. On the basis of homology of deduced amino acids, the RT sequences could be grouped into three major families. The intra-family divergence at amino acid level ranges from 2 to 19%. Though intra-family RT sequences were conserved but no two sequences were identical. The results indicate a high degree of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Plant Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/697/version/1

Hierarchical coexistence of universality and diversity controls robustness and multi-functionality in intermediate filament protein networks
Provedor de dados: 17
Autores: Theodor Ackbarow; Markus  J. Buehler.
Proteins constitute the elementary building blocks of a vast variety of biological materials such as cellular protein networks, spider silk or bone, where they create extremely robust, multi-functional materials by self-organization of structures over many length- and time scales, from nano to macro.  Some of the structural features are commonly found in a many different tissues, that is, they are highly conserved.  Examples of such universal building blocks include alpha-helices, beta-sheets or tropocollagen molecules.  In contrast, other features are highly specific to tissue types, such as particular filament assemblies, beta-sheet nanocrystals in spider silk or tendon fascicles. These examples illustrate that the coexistence of universality and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Ecology; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/826/version/1

Hierarchical nanomechanics of collagen microfibrils
Provedor de dados: 17
Autores: Alfonso Gautieri; Simone  Vesentini; Alberto Redaelli; Markus J. Buehler.
Collagen constitutes one third of the human proteome, providing mechanical stability, elasticity and strength to connective tissues. Collagen is also the dominating material in the extracellular matrix (ECM) and is thus crucial for cell differentiation, growth and pathology. However, fundamental questions remain with respect to the origin of the unique mechanical properties of collagenous tissues, and in particular its stiffness, extensibility and nonlinear mechanical response. By using x-ray diffraction data of a collagen fibril reported by Orgel et al. (Proceedings of the National Academy of Sciences USA, 2006) in combination with protein structure identification methods, here we present an experimentally validated model of the nanomechanics of a...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4995/version/1

Hierarchical nanomechanics of collagen microfibrils
Provedor de dados: 17
Autores: Alfonso Gautieri; Simone  Vesentini; Alberto Redaelli; Markus J. Buehler.
Collagen constitutes one third of the human proteome, providing mechanical stability, elasticity and strength to organisms and is thus the prime construction materials in biology. Collagen is also the dominating material in the extracellular matrix and its stiffness controls cell differentiation, growth and pathology. However, the origin of the unique mechanical properties of collagenous tissues, and in particular its stiffness, extensibility and nonlinear mechanical response remains unknown. By using x-ray diffraction data of a collagen fibril (Orgel et al., PNAS, 2006) here we present an experimentally validated model of the nanomechanics of a collagen microfibril that incorporates the full biochemical details of the amino acid sequence of constituting...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4995/version/2

High efficiency transfection of thymic epithelial cell lines and primary thymic epithelial cells by Nucleofection
Provedor de dados: 17
Autores: Richard T. O&amp;#x27;Neil; Qiaozhi Wei; Brian G. Condie.
Thymic epithelial cells (TECs) are required for the development and differentiation of T cells and are sufficient for the positive and negative selection of developing T cells. Although TECs play a critical role in T cell biology, simple, efficient and readily scalable methods for the transfection of TEC lines and primary TECs have not been described. We tested the efficiency of Nucleofection for the transfection of 4 different mouse thymic epithelial cell lines that had been derived from cortical or medullary epithelium. We also tested primary mouse thymic epithelial cells isolated from fetal and postnatal stages. We found that Nucleofection was highly efficient for the transfection of thymic epithelial cells, with transfection efficiencies of 30-70% for...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6283/version/1

High energy-charged cell factory for heterologous protein synthesis
Provedor de dados: 17
Autores: Pil Kim; Hye Jung Kim; Yeong Deok Kwon; Sang Yup Lee.
Overexpression of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) under glycolytic conditions enables Escherichia coli to maintain a greater intracellular ATP concentration and, consequently, to up-regulate genes for amino acid and nucleotide biosynthesis. To investigate the effect of a high intracellular ATP concentration on heterologous protein synthesis, we studied the expression of a foreign gene product, enhanced green fluorescence protein (eGFP), under control of the T7 promoter in E. coli BL21(DE3) strain overexpressing PCK. This strain was able to maintain twice as much intracellular ATP and to express two times more foreign protein than the control strain. These results indicate that a high energy-charged cell can be beneficial as a...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3953/version/1

Higher accuracy protein Multiple Sequence Alignment by Stochastic Algorithm
Provedor de dados: 17
Autores: M. S. Jeevitesh; Narayan Behera; Justin Jose; Krishna Kant; Alpana Dey.
Multiple Sequence Alignment gives insight into evolutionary, structural and functional relationships among the proteins. Here, a novel Protein Alignment by Stochastic Algorithm (PASA) is developed. Evolutionary operators of a genetic algorithm, namely, mutation and selection are utilized in combining the output of two most important sequence alignment programs and then developing an optimized new algorithm. Efficiency of protein alignments is evaluated in terms of Total Column score which is equal to the number of correctly aligned columns between a test alignment and the reference alignment divided by the total number of columns in the reference alignment. The PASA optimizer achieves, on an average, significant better alignment over the well known...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4258/version/1

Highly Efficient Gene Expression in B Lymphocytes Mediating by Lentivirus Vector
Provedor de dados: 17
Autores: Akira Tempaku.
Gene transduction and expression efficiencies among several type cell lines were compared by using vesicular stomatitis virus-glycoprotein (VSV-G) pseudotyped human immunodeficiency virus type-1 (HIV-1) based lentivirus vector. Large discrepancies of the efficiencies were shown among them. B lymphocytes showed high susceptibility of gene transduction and expression, while other cell lines marked lower potential. Variable gene transduction strategies have been assessed to apply immunological therapies. This study showed that B lymphocytes had facilities enough to support the gene transduction and expression by lentivirus vector. Our result suggested that the lentivirus vector would be a powerful tool to express exogenous genes in B lymphocytes.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology.
Ano: 2012
URL: http://precedings.nature.com/documents/7030/version/1

High-Throughput Transcriptome Profiling Of Human Mesenchymal Stem  Cells Reveals A Role For Wnt/GSK-3 Signaling In Their Hypoimmunomodulation
Provedor de dados: 17
Autores: Gurudutta Gangenahalli; Neeraj Satija; Farhat Afrin; Rajender Prasad Tripathi.
Mesenchymal stem cells (MSCs) are gaining importance among clinicians following recent demonstration of safe allogeneic transplantation due to their ability to modulate the immune response. However,the molecular machinery regulating the expression of immunomodulatory factors in MSCs is unknown. We, therefore, inhibited glycogen synthase kinase-3 (GSK-3),a Wnt signaling inhibitor, to elucidate the role of Wnt signaling in mediating immunoregulatory effects of human MSCs using gene expression profiling. Our results highlight enhanced ability of GSK-3 inhibitor (lithium) treated MSCs to evade immune response as a result of decreased expression of immune stimulatory cytokines and chemokines.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Immunology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5512/version/1

High-Value Phytochemicals from Grape Cane Waste: Potential Value-Added Viticultural Sources of Trans-Resveratrol and Trans-e-Viniferin with Medicinal and Anti-Phytopathogenic Applications
Provedor de dados: 17
Autores: Sierra Rayne.
Grape canes as agricultural waste from commercial viticultural activities represent a potentially important source of the well-known medicinal and anti-phytopathogenic stilbene compounds trans-resveratrol and trans-e-viniferin. Reports in the literature suggest that concentrations of these compounds range up to 5 mg/g dw and 2 mg/g dw, respectively, and can be quantitatively extracted from the cane residue using low-cost, environmental benign, and non-toxic aqueous alcoholic solvent systems such as ethanol:water mixtures. With current commercial values of  these compounds between US$2,000 to US$3,000 per kg, established stilbene yields from cane waste could represent an agricultural coproduct valued at US$2,000 to US$3,000 per hectare of production. At the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Earth &amp; Environment.
Ano: 2007
URL: http://precedings.nature.com/documents/636/version/1

Histórico da Comissão Interna de Biossegurança da Embrapa Cerrados.
Provedor de dados: 14
Autores: FALEIRO, F. G.; ANDRADE, S. R. M. de; SOUZA, P. I. de M.; MOREIRA, C. T.; ABUD, S.; MENDES, I. de C.; REIS JÚNIOR, F. B. dos.
bitstream/CPAC-2009/26860/1/p2005_51.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Transgênico; Transgenics.; Biotecnologia; Genética Vegetal; Melhoramento Genético Vegetal.; Genetics; Biotechnology; Plant breeding.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/569168

HMGB1 release by C5a anaphylatoxin is an effective target for sepsis treatment
Provedor de dados: 17
Autores: Hidechika Okada; Noriko Okada; Masaki Imai; Alan Okada; Fumiko Ono.
Antibodies to C5a have proven to be effective in treating experimental septic primate models. A 17 amino acid peptide (ASGAPAPGPAGPLRPMF) named PepA binds to C5a and prevents complement-mediated lethal shock in rats. AcPepA harboring an acetyl group at the N-terminal alanine showed increased inhibitory activity against C5a. Cynomolgus monkeys destined to expire from a lethal dose of bacterial endotoxin (4mg/kg) were rescued by intravenous administration of AcPepA. AcPepA could have interfered with the ability of C5a to stimulate C5L2 which is responsible for HMGB1 release and stimulation of TLR4 as an endogeneous ligand with LPS behavior. The suppression of HMGB1 release by AcPepA administration to LPS-shock monkeys is likely responsible for rescuing the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/5727/version/1

Homology Modeling of Human &amp;#x3b1;~2A~-Adrenoceptor
Provedor de dados: 17
Autores: Jignesh Patel; Gourja G; Vivek Kumar; Yogesh Narkhede; Shikhar Gupta; C. Gopi Mohan.
&amp;#x3b1;~2A~-adrenoceptor (ADA2A) is a membrane bound receptor which has been classified as the member of larger superfamily G-protein coupled receptors (GPCRs); also known as seven transmembrane (7-TM) domains receptor. Around 50% drugs currently available in the market exert their effects through GPCRs. Membrane proteins are difficult to crystallize as compared to soluble proteins. There is a need of 3D-structure of ADA2A to understand   binding modes of various agonists and antagonists and hence homology model of ADA2A was developed. Homology model of ADA2A constructed based on crystal structure of &amp;#x3b2;~2~-adrenoceptor. The crystal structure of &amp;#x3b2;~2~-adrenoceptor (PDB ID: 2RH1) was used as template, which has good sequence identity...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/4106/version/1

Hongos comestibles silvestres en una Zona Otomí y dinámica de colonización ectomicorrízica entre Pinus pseudostrobus y Hebeloma mesophaeum.
Provedor de dados: 32
Autores: Heredia Acuña, Cristina.
Dentro de los ecosistemas forestales existe una amplia diversidad de microorganismos que participan en el establecimiento del equilibrio del sistema, dentro de los cuales se encuentran de manera relevante, los hongos ectomicorrízicos. Los hongos ectomicorrízicos forman asociaciones simbióticas con especies forestales, en esta simbiosis existe un intercambio de nutrimentos esenciales para la vida de ambos organismos. Desde el punto de vista antropocéntrico existen hongos ectomicorrízicos que han sido utilizados desde épocas prehispánicas como alimento o medicina, en la actualidad existe un gran interés por validar y ampliar dichos conocimientos desde una perspectiva científica. Diversas especies han sido estudiadas con la finalidad de evaluar su potencial...
Palavras-chave: Etnomicología; Biotecnología; Ectomicorríza; Hebeloma mesophaeum; Pinus ayacahuite; Pinos mexicanos; Ethnomycology; Biotechnology; Ectomycorrhiza; Mexican pines; Maestría; Edafología.
Ano: 2011
URL: http://hdl.handle.net/10521/495

Hongos comestibles silvestres en una Zona Otomí y dinámica de colonización ectomicorrízica entre Pinus pseudostrobus y Hebeloma mesophaeum.
Provedor de dados: 32
Autores: Heredia Acuña, Cristina.
Dentro de los ecosistemas forestales existe una amplia diversidad de microorganismos que participan en el establecimiento del equilibrio del sistema, dentro de los cuales se encuentran de manera relevante, los hongos ectomicorrízicos. Los hongos ectomicorrízicos forman asociaciones simbióticas con especies forestales, en esta simbiosis existe un intercambio de nutrimentos esenciales para la vida de ambos organismos. Desde el punto de vista antropocéntrico existen hongos ectomicorrízicos que han sido utilizados desde épocas prehispánicas como alimento o medicina, en la actualidad existe un gran interés por validar y ampliar dichos conocimientos desde una perspectiva científica. Diversas especies han sido estudiadas con la finalidad de evaluar su potencial...
Palavras-chave: Etnomicología; Biotecnología; Ectomicorríza; Hebeloma mesophaeum; Pinus ayacahuite; Pinos mexicanos; Ethnomycology; Biotechnology; Ectomycorrhiza; Mexican pines; Maestría; Edafología.
Ano: 2011
URL: http://hdl.handle.net/10521/495

Human Cancer Cells Express Slug-based Epithelial-Mesenchymal Transition Gene Signature Obtained in Vivo
Provedor de dados: 17
Autores: Dimitris Anastassiou; Viktoria Rumjantseva; Wei-yi Cheng; Jianzhong Huang; Peter D. Canoll; Darrell J. Yamashiro; Jessica J. Kandel.
The biological mechanisms underlying cancer cell motility and invasiveness remain unclear, although it has been hypothesized that they involve some type of epithelial-mesenchymal transition (EMT). Here we show that human cancer cells express in vivo a precise multi-cancer invasion-associated gene expression signature characterized by the prominent presence of collagen COL11A1 and thrombospondin THBS2. The signature is present in the expression of all solid tumor datasets that we analyzed and includes numerous EMT markers, among them the transcription factor Slug, fibronectin, and &amp;#x3b1; SMA. Using xenograft models of human cancer cells in immunocompromised mice and profiling the harvested tumors separately with species specific probes, we found that...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6243/version/1

Hybrid Extraction Method; Innovative Method to Produce Non Oxidative Tuna Oil
Provedor de dados: 17
Autores: Yoshinori Ito.
Summary: The function of Polyunsaturated Fatty Acid such as DHA, EPA are now well known world widely through strong scientific evidences and the importance of nutritional value and popularity are increasing every day all over the world with the name of omega 3 fatty acids.^1,2,3^ However, the fish oil, the source of omega 3 fatty acid had been considered as very vulnerable to oxidation. Many papers say the reason why is because its very easy to oxidize fish oil. The greater the Polyunsaturated Fatty Acid contents in the fish oil is, the faster oxidation takes place.^4,5^ Here I show that tuna oil extracted from Albacore Tuna Head in closed circuit under low temperature, at first decompressing then pressurizing and heating in a container, is very stable...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology.
Ano: 2009
URL: http://precedings.nature.com/documents/3110/version/1

ICRISAT in the 21st century: towards sustainable food security
Provedor de dados: 30
Palavras-chave: Pigeonpeas; Genetic resources; Postharvest technology; Millets; Biotechnology; Green revolution; Tropical zones; Hybrids; Policies; Integrated pest management.
Ano: 1998
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4210

Identification of a highly polymorphic tetranucleotide repeat locus (DXpS) at Xp and development of a DXpS/HUMARA biplex methylation-based PCR assay that enhances detection of X-chromosome inactivation
Provedor de dados: 17
Autores: Filipe B. Machado; Ester S. Ramos; Pamela C.  Radic; Carlos D. de Brasi; Enrique Medina-Acosta.
The methylation-based PCR assay at the polymorphic (CAG)n repeat in exon 1 of the human androgen receptor _AR_ gene (HUMARA) is a standard method for determination of the methylation state of alleles in active X (Xa) and inactive X (Xi) chromosomes. HUMARA assay is endowed with heterozygosity rates ~85% worldwide. This means that in a proportion of females it is uninformative. The HUMARA genotype is not neutral, being linked to Kennedy&amp;#xb4;s disease. Moreover, allele designation and quantification from trinucleotide repeats demand normalizing for minor (stutter) products differing from the original template by multiples of the repeat unit. Here, we report on the identification of a highly polymorphic tetranucleotide repeat (named DXpS) mapping to...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics &amp; Genomics.
Ano: 2011
URL: http://precedings.nature.com/documents/6633/version/1

Identity in research infrastructure and scientific communication: Report from the 1st IRISC workshop, Helsinki Sep 12-13, 2011
Provedor de dados: 17
Autores: Gudmundur A. Thorisson; Mikael  Linden; Anthony J. Brookes; Myles Byrne; Juha Muilu; Tommi Nyronen.
Motivation for the IRISC workshop came from the observation that identity and digital identification are increasingly important factors in modern scientific research, especially with the now near-ubiquitous use of the Internet as a global medium for dissemination and debate of scientific knowledge and data, and as a platform for scientific collaborations and large-scale e-science activities.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The 1 1/2 day IRISC2011 workshop sought to explore a series of interrelated topics under two main themes: i) unambiguously identifying authors/creators &amp;#x26; attributing their scholarly works, and ii) individual identification and access management in the context of identity federations. Specific aims of the workshop...
Tipo: Manuscript
Palavras-chave: Biotechnology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6609/version/1

IDENTITY PRESERVATION AND LABELING OF GENETICALLY MODIFIED PRODUCTS: SYSTEM DESIGN AND ENFORCEMENT ISSUES
Provedor de dados: 31
Autores: Moschini,   GianCarlo; Lapan,   Harvey E.
This paper analyzes economic issues that arise in devising a credible and enforceable system of identity preservation and labeling for genetically modified (GM) and non-GM products. The model represents three stages in the supply chain: farm production, marketing handlers, and final users. The possibility of accidental co-mingling of non-GM products is modeled at the marketing stage. Regulation takes the form of a threshold level of purity for non-GM products, a probability of government testing to verify compliance with the threshold level, and a fine for violators. Uncertainty is modeled explicitly, such that would-be suppliers of non-GM products always face some risk of failing the test and incurring a fine. The paper also presents a novel demand...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Enforcement; Food labeling; Identity preservation; Regulation; Uncertainty; Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/18355

Impact of Allium sativum leaf lectin on the Helicoverpa armigera larval parasitoid Campoletis chlorideae
Provedor de dados: 30
Autores: Arora, R.,( Richa Arora ).
Palavras-chave: Animal developmental stages; Parasites; Transgenics; Biological interaction; Chickpeas; Biotechnology; Genes; Garlic; Proteins; Grain legumes.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3288

Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions
Provedor de dados: 17
Autores: Hiroyuki Koyama; Hideo Kagaya; Makoto Oba; Yutaka Miura; Takehiko Ishii; Tsuyoshi Takato; Kazunori Kataoka; Tetsuro Miyata.
Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (cRGD) was introduced to block copolymer, poly(ethylene glycol)-block-polycation carrying ethylenediamine units (PEG-PAsp(DET)). cRGD recognizes &amp;#x3b1;v&amp;#x3b2;3 and &amp;#x3b1;v&amp;#x3b2;5 integrins, which are abundantly expressed in vascular lesions. cRGD-conjugated PEG-PAsp(DET) (cRGD-PEG-PAsp(DET)) formed polyplex micelles through complexation with plasmid DNA (pDNA), and the cRGD-PEG-PAsp(DET) micelles achieved significantly more efficient gene expression and cellular uptake as compared with...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5020/version/1

IMPACTS OF GROWTH STIMULANTS IN THE DOMESTIC LIVESTOCK SECTOR
Provedor de dados: 31
Autores: Peterson,   Everett B.; Preckel,   Paul V.; Hertel,   Thomas W.; McGuirk,   Anya M.
Many individuals are predicting a "second green revolution" in agriculture from the introduction of growth stimulants into the livestock industries. An economy-wide approach is used to determine the affects on prices and quantities of introducing growth stimulants in the domestic dairy and pork industries. In general, the impacts are found to be much smaller than previous research focused at the sectoral level has suggested. Increases in the amount of lean meat per hog, which reduces processor demand, may actually lead to a reduction in pork production at the farm-level.
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; General equilibrium; Pork and dairy; Livestock Production/Industries.
Ano: 1992
URL: http://purl.umn.edu/116106

Improved prediction of protein interaction from microarray data using asymmetric correlation
Provedor de dados: 17
Autores: Kojiro Yano.
Background:Detection of correlated gene expression is a fundamental process in the characterization of gene functions using microarray data. Commonly used methods such as the Pearson correlation can detect only a fraction of interactions between genes or their products. However, the performance of correlation analysis can be significantly improved either by providing additional biological information or by combining correlation with other techniques that can extract various mathematical or statistical properties of gene expression from microarray data. In this article, I will test the performance of three correlation methods-the Pearson correlation, the rank (Spearman) correlation, and the Mutual Information approach-in detection of protein-protein...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4430/version/1

Improving cellular cancer vaccines
Provedor de dados: 17
Autores: Petr Lokhov.
Immunization with cancer cells is of great demand in anti-cancer therapy. However, current cellular vaccines are inefficient and there are questions regarding their overall safety. We report a simple and straightforward approach for improving of cellular cancer vaccines. Through treatment of cancer cell cultures with purified protease, it is possible to make preparations of cell-surface antigens that are free of intracellular content and contain two orders-of-magnitude less protein than the whole lysate of an equivalent number of cancer cells. Despite this difference in total protein content, protease-generated preparations stimulate anti-cancer responses from immune cells better those stimulated with cancer cells themselves. The composition of collected...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4356/version/1

In silico Genetic Network Models for &amp;#xb;Pre-clinical Drug Prioritization
Provedor de dados: 17
Autores: Jianghui Xiong; Juan Liu; Simon Rayner; Ze Tian; Yinghui Li; Shanguang Chen.
The high rates of failure in oncology drug clinical trials highlight the problems of using pre-clinical data to predict the clinical effects of drugs. Patient population heterogeneity and unpredictable physiology complicate pre-clinical cancer modeling efforts. We hypothesize that gene networks associated with cancer outcome in heterogeneous patient populations could serve as a reference for identifying drug effects. Here we propose a novel in vivo genetic interaction which we call &amp;#x2018;synergistic outcome determination&amp;#x2019; (SOD), a concept similar to &amp;#x2018;Synthetic Lethality&amp;#x2019;. SOD is defined as the synergy of a gene pair with respect to cancer patients&amp;#x27; outcome, whose correlation with outcome is due to...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5343/version/1

In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production
Provedor de dados: 17
Autores: Israr  Khan; Muhammad Waheed Akktar; Ayaz Ali Khan.
According to the &amp;#x22;RNA World Hypothesis&amp;#x22; the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity, fidelity and generality of the enzyme.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6064/version/1

In vitro organogenesis from internodal segments of adult sweet orange plants
Provedor de dados: 86
Autores: Bassan,Meire Menezes; Mourão Filho,Francisco de Assis Alves; Miyata,Luzia Yuriko; Mendes,Beatriz Madalena Januzzi.
The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Citrus sinensis; Adventitious buds; Biotechnology; Plant growth regulators.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000600015

In vitro shoot regeneration from cotyledon of redgram
Provedor de dados: 17
Autores: Telugu Raghavendra; Palagiri Sudhakar; K Balakrishna Reddy; Keerthi Venkaiah; Putta Latha; Tollamadugu NVKV Prasad; Lakkireddi Prasanthi.
Pigeonpea is one of the most popular legume grains in the world, especially in the Indian sub-continent. Keeping in view the importance of the crop, an efficient protocol for rapid in vitro plant regeneration from cotyledon of redgram has been reported in this paper. The concentraion of the hormones played an important role in shoot and root regeneration.
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7041/version/1

_In vivo_ photoacoustic molecular imaging with simultaneous multiple selective targeting using antibody-conjugated gold nanorods
Provedor de dados: 17
Autores: Pai-Chi Li; Dar-Bin Shieh; Churng-Ren Wang; Chen-Wei Wei; Chao-Kang Liao; Ann-Ann Ding; Ya-Na Wu; Carolina Poe; Suwen Jhan.
The use of gold nanorods for photoacoustic molecular imaging in vivo with simultaneous multiple selective targeting is reported. The extravasation of multiple molecular probes is demonstrated, and used to probe molecular information of cancer cells. This technique allows molecular profiles representing tumor characteristics to be obtained and a heterogeneous population of cancer cells in a lesion to be determined. The results also show that the image contrast can be enhanced by using a mixture of different molecular probes. In this study, HER2, EGFR, and CXCR4 were chosen as the primary target molecules for examining two types of cancer cells, OECM1 and Cal27. OECM1 cells overexpressed HER2 but exhibited a low expression of EGFR, while Cal27 cells showed...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1687/version/1

Incorporating Collateral Information Using an Adaptive Management Framework for the Regulation of Transgenic Crops
Provedor de dados: 31
Autores: Linacre,   Nicholas A.; Burgman,   Mark A.; Ades,   Peter K.; Stewart-Oaten,   Allen.
A lack of data often makes biological management decisions difficult and has been an area of contention in the debate over the approval of transgenic crops. Our knowledge of natural systems is limited and our ability to gain additional information, quickly and effectively, is often handicapped by statistical complexity. To adequately cope with this requires new approaches and models that integrate decision-making and management. This paper describes one possible approach to the integration of decision-making and management, which may have application for the regulatory approval of transgenic crops. In many situations countries wishing to approve transgenic crops will have limited data on the environmental performance of the crop. The approach outlined in...
Tipo: Working or Discussion Paper
Palavras-chave: Bayesian estimation; Collateral data; Inference; GMO; Transgenic plants; Bayesian theory; Biotechnology; Biosafety; Regulatory systems; Crop Production/Industries.
Ano: 2005
URL: http://purl.umn.edu/59233

Incorporating Project Uncertainty in Novel Environment Biotechnologies: Illustrated Using Phytoremediation
Provedor de dados: 31
Autores: Linacre,   Nicholas A.; Whiting,   Steven N.; Angle,   J. Scott.
Pollution of the environment by metals and organic contaminants is an intractable global problem, with cleanup costs running into billions of dollars using current engineering technologies. The availability of alternative, cheap and effective technologies would significantly improve the prospects of cleaning-up metal contaminated sites. Phytoremediation has been proposed as an economical and ‘green’ method of exploiting plants to extract or degrade the contaminants in the soil. To date, the majority of phytoremediation efforts have been directed at leaping the biological, biochemical and agronomic hurdles to deliver a working technology, with scant attention to the economic outlook other than simple estimates of the cost advantages of phytoremediation over...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Project risk; Soil contaminants; Environmental remediation; Industrial crop technologies; Crop Production/Industries; Q5; Q51; Q2.
Ano: 2005
URL: http://purl.umn.edu/59223

Increased regeneration efficiency of _Brassica napus_ L. cultivars Star, Westar and Cyclone from hypocotyle and cotyledonary explants
Provedor de dados: 17
Autores: Israr Khan; Waqar  Ali; Zahir Ali Takar; Arfa Farooqi; Sikandar Khan; Muhammadwaheed Akhtar.
The comparative organogenesis of _Brassica napus_ L cultivars Cyclone, Star and Westar was studied. The cotyledonary explants gave a higher response to all the combinations of 0.5 mg/L 2,4-D and BAP (0.5, 1.0,1.5 and 2.0 mg/L} used for optimizing the conditions for callus induction. The best mean weight and mean length of callus was obtained at 0.5 mg/L 2,4-D and 1.5mg/L BAP for Star cotyledonary explants. For the complete plant regeneration the new method of exposing the explants culture to Growth regulator free medium was performed. The method was applicable to both hypocotyl and cotyledonary explants. The Shoot Induction Frequency for hypocotyl (6-34%) in the three cultivars is higher than the cotyledonary explants (3-23%). The method is speedy and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4781/version/1

Increasing Access To Bioinformatics Resources: Increase Community Curation by Increasing Your Community
Provedor de dados: 17
Autores: Jennifer M. Williams.
The amount of biological data has risen exponentially over the last decade. Along with this rise, the number and types of bioinformatics resources has risen such that the sheer number of bioinformatics resources is overwhelming. For these resources to attain their full potential, they must be efficiently and extensively utilized. But in such a plethora of resources, how does a researcher new to a field find the resources that will meet their needs. Once a resource is found, how does the researcher quickly learn to utilize that resource fully? There are resource lists such as those provided by the journal Nucleic Acid Research (NAR), BioMed Central and the Univ. of Pittsburgh Health Sciences Library, and most resources include their own documentation. But...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3163/version/1

Indian Farmers'  Valuation of Crop Yield Distributions: Will poor farmers value 'pro-poor' seeds?
Provedor de dados: 31
Autores: Lybbert,   Travis J.
Potential poverty traps among the rural poor suggest a need to reduce poor farmers' vulnerability by stabilizing crop yields and limiting yield losses. Advances in agricultural biotechnology enable breeders to address this need more directly than ever before with crops that reduce production risk by tolerating climate fluctuation or resisting biotic stresses. Will poor farmers who could benefit most from less vulnerability choose to purchase such risk-reducing seeds? I use data from a household survey and experiment involving farmers in India to infer their valuation of changes in the mean, variance, and skewness of yield distributions. I conclude that these farmers value increases in expected yield in the yield distribution but seem indifferent about...
Tipo: Conference Paper or Presentation
Palavras-chave: Poverty; Risk; Biotechnology; Experimental Economics; Farm Management; C9–; D8; O1; Q1.
Ano: 2005
URL: http://purl.umn.edu/19160

Induced fit, conformational selection and independent dynamic segments: an extended view of binding events
Provedor de dados: 17
Autores: Peter Csermely; Robin Palotai; Ruth Nussinov.
Single molecule and NMR measurements of protein dynamics increasingly uncover the complexity of binding scenarios. Here we describe an extended conformational selection model which embraces a repertoire of selection and adjustment processes. Induced fit can be viewed as a subset of this repertoire, whose contribution is affected by the bond-types stabilizing the interaction and the differences between the interacting partners. We argue that protein segments whose dynamics are distinct from the rest of the protein (&amp;#x2018;discrete breathers&amp;#x2019;) can govern conformational transitions and allosteric propagation that accompany binding processes, and as such may be more sensitive to mutational events. Additionally, we highlight the dynamic...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4422/version/1

INFORMATION POLICY AND GENETICALLY MODIFIED FOOD: WEIGHING THE BENEFITS AND COSTS
Provedor de dados: 31
Autores: Teisl,   Mario F.; Caswell,   Julie A.
The labeling of genetically modified foods (GMFs) is the topic of a debate that could dramatically alter the structure of the U.S. and international food industry. The current lack of harmonization of policy across countries makes GMF labeling an international trade issue. The U.S. and Canada do not require GMFs to be labeled unless the GMF is significantly different than the conventional food or the GMF presents a health concern. However, many other countries are requiring GMFs to be labeled. This paper discusses empirical work on the sources and magnitude of benefits and costs from labeling programs, with particular emphasis on the impact of the design of the labeling program on benefits and costs.
Tipo: Working or Discussion Paper
Palavras-chave: GMOs; Biotechnology; Labeling; Benefits; Costs; Food Consumption/Nutrition/Food Safety; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/14534

Inhibition of pteridine reductase 1 (PTR1) expression in Leishmania promastigotes using a full-length antisense construct
Provedor de dados: 17
Autores: Farnaz Kheirandish; Mojgan Bandehpour; Nushin Davoudi; Nariman Mosaffa; Fereydoun Mahboudi; Bahram Kazemi; Ali Haghighi; Mehdi Mohebali.
Leishmania exhibit many unusual features, one of which is the pteridine metabolic pathway essential for the growth for these parasites. Leishmania have evolved a complex and versatile pteridine salvage network capable of scavenging a wide array of conjugated and unconjugated pteridines. The L. major PTR1 gene was cloned into pcDNA3 digested with KpnI and BamHI. The gene was cloned antiparallel to the promoter and named pcDNA-Rptr. L. major promastigotes were divided into two groups. One group was transfected with 50 &amp;#x3bc;g of pcDNA-Rptr, whereas the other group was electroporated with pcDNA3. Mentioned cells were cultured and plated onto semi-solid media.Western blotting was performed on extracts from transfected promastigotes of L. major using an...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2807/version/1

Integration of the scientific literature into the Semantic Web: Facts from biomedical data resources
Provedor de dados: 17
Autores: Dietrich Rebholz; Samuel Croset; Christoph Grabmueller.
Scientific literature is the primary resource for relevant and innovative information. The integration of the literature with other data resources in the biomedical research community generates overhead that can be avoided through the used of Semantic Web Technology generating openly accessible data. Projects such as SESL and CALBC have producted significant amount of data that that are ready for exploitation.&amp;#xd;&amp;#xa;The tutorial will teach different approaches on how to integrate the scientific literature with the content from biomedical databases, and will discuss the inferences that can be achieved. Furthermore, the tutorial will point to the resources that are ready for use and enable integration of the literature at your discretion. A good...
Tipo: Presentation
Palavras-chave: Biotechnology; Immunology; Bioinformatics; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7011/version/1

Intellectual property rights related to the genetically modified glyphosate tolerant soybeans in Brazil
Provedor de dados: 42
Autores: Rodrigues,Roberta L; Lage,Celso L. S; Vasconcellos,Alexandre G.
The present work analyzes the different modalities of protection of the intellectual creations in the biotechnology agricultural field. Regarding the Brazilian legislations related to the theme (the Industrial Property Law - no. 9. 279/96 and the Plant Variety Protection Law - no. 9. 456/97), and based in the international treaties signed by Brazil, the present work points to the inclusions of each of them, as well as to their interfaces using as reference the case study of glyphosate tolerant genetically modified soybean. For this case study, Monsanto's pipelines patents were searched and used to analyze the limits of patent protection in respect to others related to the Intellectual Property (IP) laws. Thus, it was possible to elucidate the complex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; GMO; Intellectual property; Patent; Pipeline; Soybean.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200029

Intelligence-based medicine
Provedor de dados: 17
Autores: Pierre Squara; Didier Journois.
Despite seven hundred thousand new medical references last year, the relationship between a given set of medical features and specific pathophysiology, treatment, and criteria of improvement is often weak. Moreover, the generalization of evidences obtained in specific settings may lead to under-treat or to over-treat a significant proportion of patients. We expose an application of the cybernetic loop, based on traditional medical steps: nosology, semeiology, pathophysiology, therapy and on the four transitions between these steps. This approach leads to formulate eight basic questions evaluating the steps in terms of reproducibility and the transitions in terms of predictivity. We detail two practical applications: 1) the evaluation of a medical decision...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3196/version/1

Intestinal Electric Stimulation Accelerates Whole Gut Transit and Promotes Fat Excrement in Conscious Rats
Provedor de dados: 17
Autores: Ying Sun; Jiande Chen.
*_Introduction:_* Intestinal electric stimulation (IES) is proposed as a potential tool for the treatment of morbid obesity. Our previous study showed that IES with one pair of electrodes accelerated intestinal transit and decreased fat absorption in a segment of the jejunum in the anesthetized rats. The aims of this study were to assess the effects of IES on the whole gut transit and fat absorption in conscious rats, to examine the effects of multi-channel IES, and to explore the cholinergic mechanism behind the effects of IES. &amp;#xd;&amp;#xa;*_Methods:_* Thirty-eight male rats implanted with serosal electrodes were randomized into five groups: control without IES, 2/3 channel IES with short pulses, atropine and atropine plus IES. The whole gut transit...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2854/version/1

Intra-mammary single infusion of casein hydrolyzate in dairy cows as alternative dry period treatment: Curing bacterial infection and increasing milk yield in the subsequent lactations
Provedor de dados: 17
Autores: Nissim Silanikove; Shamay Yacoby; Gabriel Leitner.
Conclusions: This study sustain previous results that treatment with CNH is a powerful mean to eradicated bacterial infection, and strongly highlight the potential of this method to serve as a viable non-antibiotic dry cow therapy treatment. The increase in milk yield is a valuable bonus and its effectiveness is comparable with that achieved with growth hormone treatment, albeit in much simple mode of treatment and without the problematic of hormonal treatment.
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2130/version/1

Invasive monitoring of the clinical effects of high intra-abdominal pressure for insertion of the first trocar.
Provedor de dados: 17
Autores: Octavio H. M. Hypolito; Joao-Luiz Azevedo; Fernanda   A. B. Gama; Otavio  C. Azevedo; Otavio M. Becker Junior; Afonso C. C. G. Machado; Susana A. Miyahira; Glicia   C. Azevedo; Bianca Marigliani; Jos&amp;#xe9;  F. Borborema; Linda  Bernardes; Gustavo P. S. Miguel.
Background: To analyze the effects of transitory, high intra-abdominal pressure on clinical, hemodynamic, blood gas and metabolic parameters.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;Methods: Sixty-seven laparoscopic patients were divided into groups P12 (n = 30, maximum intra-abdominal pressure of 12 mmHg) and P20 (n = 37, maximum intra-abdominal pressure of 20 mmHg). Through radial artery cannulation, mean arterial pressure (MAP) was assessed and blood gas analysis &amp;#x2013; pH, arterial oxygen tension (PaO2), arterial carbon dioxide tension (PaCO2), bicarbonate (HCO3) and base excess (BE) &amp;#x2013; was performed. These parameters were evaluated in both groups at time point zero, before CO2 insufflation; at time point one (TP1), when intra-abdominal...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6536/version/1

In-Vivo Lipidomics using Single-Cell Raman Spectroscopy
Provedor de dados: 17
Autores: Huawen Wu; Joanne Volponi; Ann Oliver; Atul Parikh; Blake Simmons; Seema Singh.
We describe a method for direct, quantitative, in vivo lipid profiling of oil producing microalgae using single-cell laser-trapping Raman spectroscopy (LTRS). This approach is demonstrated in the quantitative determination of the degree of unsaturation and transition temperatures of constituent lipids within microalgae. These properties are important markers for determining engine compatibility and performance metrics of algal biodiesel. We show that these factors can be directly measured from a single living microalgal cell held in place with an optical trap while simultaneously collecting Raman data. Cellular response to different growth conditions is monitored in real time. Our approach circumvents the need for lipid extraction and analysis that is both...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4428/version/1

Iridium complex, a phosphorescent light-emitting diode material, serves as a novel chemical probe for imaging hypoxic tumor tissues
Provedor de dados: 17
Autores: Shaojuan Zhang; Masahiro Hosaka; Toshitada Yoshihara; Yasuhiko Iida; Keigo Endo; Seiji Tobita; Toshiyuki Takeuchi.
Iridium complex, a promising organic light-emitting diode for next generation television displays, emits phosphorescence. Phosphorescence is quenched by oxygen. We used this oxygen-quenching feature for imaging tumor hypoxia. Red light-emitting iridium complex Ir(btp)~2~(acac) (BTP) presented hypoxia-dependent light emission in culture cell lines, whose intensity was in parallel with HIF-1[alpha] expression. BTP was further applied to imaging five tumors (four from human origin and one from mouse origin) transplanted in athymic mice. All tumors presented a bright BTP-emitting image even 5 min after the injection. The BTP-dependent tumor image peaked at 1 to 2 h after the injection, and was then cleared from tumors within 24 h. The minimal BTP image...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2007
URL: http://precedings.nature.com/documents/1443/version/1

Iron (FeSO4) Bioavailability in Obese Subjects Submitted to Bariatric Surgery
Provedor de dados: 17
Autores: Luciana Bueno; Juliana  Pizzo; Jos&amp;#xe9; Ernesto dos Santos; Julio S&amp;#xe9;rgio Marchini; Jos&amp;#xe9; Eduardo Dutra de Oliveira.
Objective: To determine iron bioavailability in obese subjects after the ingestion of a nutritional supplement containing multiple nutrients. Methods: Fourteen persons were studied before and after bariatric surgery after the ingestion of a nutritional formulation containing 25 mg iron, 25 g fiber and 800 mg calcium. Serum iron levels were measured basally and every hour after the ingestion of 200 mL of the formulation, and serum ferritin, unsaturated iron binding capacity (UIBC), hemoglobin, albumin, total proteins, total triglycerides, total cholesterol and fractions (LDL-cholesterol and HDL-cholesterol) sere determined at the same time points.Results: The following ferremia values (median/minimum - maximum) were obtained before and after bariatric...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3787/version/1

Is Monsanto Leaving Money on the Table? Monopoly Pricing and Bt Cotton Value with Heterogeneous Adopters
Provedor de dados: 31
Autores: Oehmke,   James F.; Wolf,   Christopher A.
We examine the allocation of technology rents between a price-setting, innovating monopolist and heterogeneous technology adopters.  A model of monopoly pricing in the presence of heterogeneous adopters is used to examine conditions under which greater producer (farmer) heterogeneity leads to greater producer benefit from innovation in non-competitive markets.  An application to Bt cotton determines the profit-maximizing price of Bt cotton seed and reveals that Monsanto and Delta and Pine Land are indeed leaving money on the table in the form of unexploited profit opportunities.  However, we estimate that the presence of heterogeneous adopters explains over 80% of the rents that accrue to the farmers.
Tipo: Journal Article
Palavras-chave: Biotechnology; Bt cotton; Heterogeneous adopters; Innovation; Monopoly pricing; Technology; Valuation distribution; L1; O3; Q1.
Ano: 2004
URL: http://purl.umn.edu/43469

IS SOY MILK? THE ECONOMICS OF THE SOY MILK MARKET
Provedor de dados: 31
Autores: Dhar,   Tirtha Pratim; Foltz,   Jeremy D.
This study uses revealed preferences of consumers to study the consumer benefits from soy milk.  The study specifies and estimates structural demand and reduced form models of competition for different milk types using US supermarket scanner data.  The introduction of soy milk is used to estimate consumer benefits and valuations.  We decompose benefits into two components, competitive and variety effects.  Results show relatively small consumer benefits from soy milk.
Tipo: Conference Paper or Presentation
Palavras-chave: Demand Systems; Q-AIDS; Milk markets; Biotechnology; Soy milk; Food Labeling; Demand and Price Analysis; Q130; C300; D120; D400.
Ano: 2004
URL: http://purl.umn.edu/20337

Is there a Weed Shift in Roundup Ready Maize?
Provedor de dados: 17
Autores: Arnd Verschwele.
The cultivation of transgenic glyphosate-resistant crop varieties can simplify weed control in crops such as maize and others. On the other hand, the repeated use of broadly effective herbicides like glyphosate, especially in the same crop, can have a negative effect on biodiversity. Additionally there is a risk for a spread of more tolerant or even resistant weed species.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;It was the aim of a 6-years field study (2003-2008) to investigate weed effects of use of glyphosate in a continuous transgenic herbicide-resistant maize rotation at three sites in Germany. These sites differed significantly in terms of soil, climate and initial weed spectrum. The studies focussed on the comparison between local herbicide standards and...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4527/version/1

Island Communities and Biotechnology
Provedor de dados: 69
Autores: DaSilva,Edgar J.; Taylor,Mary.
In the run-up to the year 2000 and beyond, several island countries, especially in the Caribbean and Pacific regions, are confronted by the challenges and threats of globalization. In response, several island countries are initiating self-reliant strategies aimed at national and regional endogenous development. Amongst these strategies, the potential of biotechnology for economic development and technological growth is being tapped.
Tipo: Journal article
Palavras-chave: Biotechnology; Pacific islands; Caribbean region; MIRCENS.
Ano: 1998
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100001

Isolamento, cultivo criopreservação de células somáticas de mamíferos silvestres para formação de um banco de germoplasma.
Provedor de dados: 14
Autores: MARTINS, C. F.; CUMPA, H. C. B.; CUNHA, E. R. da; SILVA, C. G. da; GAMA FILHO, J. B. da; BORGES, L. A.
bitstream/item/100353/1/comtec-175.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Animal silvestre; Animal conservation; Genebank.; Criopreservação; Germoplasma; Mamífero.; Biotechnology; Cryopreservation; Reproduction; Wild animals.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/981278

Isolation and screening of d-limonene-resistant microorganisms
Provedor de dados: 58
Autores: Bicas,Juliano Lemos; Pastore,Gláucia Maria.
This study reports the isolation of microorganisms that are resistant to environment containing limonene, the most important residue in the citrus industry. For the isolation, samples collected from strategic places of a citrus processing plant (yellow water, entrance and exit of the bagasse tank, effluent and deteriorated fruits found in bins, machine straps, fruit washers and plant floor), some citrus fruit and mint from local market were used. The samples were incubated in rotary shaker at 30ºC/150rpm for 48h or 7 days in YM medium containing 0.1% limonene. Great part of the 112 strains recovered after 48h and the 126 strains recovered after 7 days were identified as Gram positive bacilli, followed by Gram negative bacilli, yeasts and Gram positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Limonene; Industrial by-products; Flavor; Biotechnology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300034

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE
Provedor de dados: 17
Autores: Bernd Kastenholz; David E. Garfin.
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE)
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4617/version/1

Issues, impacts, and prospects of the first transgenic crops tolerant to a herbicide. The case of glyphosate-tolerant soybean in the USA
Provedor de dados: 31
Autores: Bonny,   Sylvie.
IAAE 2009 International Conference
Tipo: Conference Paper or Presentation
Palavras-chave: Genetically modified crop; GMO; Biotechnology; Agro-economic impact; Environmental impact; Sustainability; Soybean; Pesticide; Weed; Prospects; Herbicide; Glyphosate; Agricultural and Food Policy; Crop Production/Industries; Environmental Economics and Policy; Production Economics; Research and Development/Tech Change/Emerging Technologies; Q55; O3; O13; O51.
Ano: 2009
URL: http://purl.umn.edu/51449

Knowledge about and acceptance of genetically modified organisms among pre-service teachers: a comparative study of Turkey and Slovenia
Provedor de dados: 69
Autores: Sorgo,Andrej; Ambrozic-Dolinsek,Jana; Usak,Muhammet; Özel,Murat.
Genetically modified organisms cannot be regarded as merely a topic for academic debate, since these have serious implications as a research field and for production based on genetic engineering. Public debates rarely base their arguments on elements rooted in scientific arguments and knowledge but are heavily loaded with emotions, opinions and informal reasoning. This study aimed to investigate the knowledge and acceptance of genetically modified organisms among prospective teachers in Slovenia and Turkey. Knowledge of genetic modification was measured with a two-tier instrument. The level of acceptance of genetic modification was measured with a 17-item instrument. Findings revealed that knowledge of genetics and biotechnology barely influenced the...
Tipo: Journal article
Palavras-chave: Biotechnology; Genetic engineering; Genetic modifications; Teaching.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400005

Laboratório de biotecnologia de plantas.
Provedor de dados: 14
Autores: EMBRAPA AMAZÔNIA ORIENTAL.
bitstream/item/50165/1/fd470001.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Belem; Pará; Brasil; Plants.; Instituição de Pesquisa; Laboratório; Pesquisa Agrícola; Planta.; Amazonia; Biotechnology; Research.
Ano: 2001
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/373934

Laparoscopy Pneumoperitoneum Fuzzy Modeling
Provedor de dados: 17
Autores: Otavio M. Becker Jr.; Jose E. Araujo Jr.; Joao L. M. C. Azevedo.
Abstract: Gas volume to intra-peritoneal pressure fuzzy modeling for evaluating pneumoperitoneum in videolaparoscopic surgery is proposed in this paper. The proposed approach innovates in using fuzzy logic and fuzzy set theory for evaluating the accuracy of the prognosis value in order to minimize or avoid iatrogenic injuries due to the blind needle puncture. In so doing, it demonstrates the feasibility of fuzzy analysis to contribute to medicine and health care. Fuzzy systems is employed here in synergy with artificial neural network based on backpropaga tion, multilayer perceptron architecture for building up numerical functions. Experimental data employed for analysis were collected in the accomplishment of the pneumoperitoneum in a random population of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6060/version/1

Lego DNA assembling, a simple in vitro method for constructing DNA molecule
Provedor de dados: 17
Autores: Mo Xian; Xinglin Jiang; Jianming Yang; Haibo Zhang; Huaiwei Liu; Yanning Zheng; Cong Wang; Sizheng Su.
Digestion-ligation based and recombination based methods for constructing recombinant DNA are the basic techniques in molecular biology and thus built one of the foundations for the modern life sciences. Here we describe a new strategy that can radically simplify some of the same task. The lego DNA assembling, based on strand annealing, allows in vitro assembly of multi DNA fragments in one step with precise junctions and excludes the need for any enzyme. As a proof of concept, we rapidly constructed plasmids from 4, 6 and 8 fragments with very high efficiencies (100%). And we found this method a powerful tool for synthetic biology, constructing a partial isoprene biosynthesis pathway (consisting of four genes) in 2 days. We also assembled a customized...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics.
Ano: 2010
URL: http://precedings.nature.com/documents/5147/version/1

Life fingerprints of nuclear reactions in the body of animals
Provedor de dados: 17
Autores: Jun Zeng; Ji-hui Han; Tao Sun; Ji-hui Su; Qi-yin Sun; Quan-shi Zhang; Wen-hui Xie; Ci-yi Liu; Xiao-jia Cai.
Nuclear reactions are a very important natural phenomenon in the universe. On the earth, cosmic rays constantly cause nuclear reactions. High energy beams created by medical devices also induce nuclear reactions in the human body. The biological role of these nuclear reactions is unknown. Here we show that the in vivo biological systems are exquisite and sophisticated by nature in influence on nuclear reactions and in resistance to radical damage in the body of live animals. In this study, photonuclear reactions in the body of live or dead animals were induced with 50-MeV irradiation. Tissue nuclear reactions were detected by positron emission tomography (PET) imaging of the induced beta+ activity. We found the unique tissue...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4897/version/1

Local and global modes of drug action in biochemical networks
Provedor de dados: 17
Autores: Jean-Marc Schwartz; Jose C. Nacher.
It becomes increasingly accepted that a shift is needed from the traditional target-based approach of drug development to an integrated perspective of drug action in biochemical systems. We here present an integrative analysis of the interactions between drugs and metabolism based on the concept of drug scope. The drug scope represents the set of metabolic compounds and reactions that are potentially affected by a drug. We constructed and analyzed the scopes of all US approved drugs having metabolic targets. Our analysis shows that the distribution of drug scopes is highly uneven, and that drugs can be classified into several categories based on their scopes. Some of them have small scopes corresponding to localized action, while others have large scopes...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2516/version/1

Long-term Benefits of GM crops: Potential for Diabrotica Suppression in Europe using Bt Maize
Provedor de dados: 17
Autores: Nicholas P. Storer; Peter Schlotter; Kevin L. Steffey.
Transgenic crops producing insecticidal proteins from _Bacillus thuringiensis_ (Bt) have been widely adopted since 1996 in the United States of America to combat important pests of maize and cotton. There is growing evidence that several target pest populations have been dramatically reduced in areas where the Bt crops have been most intensively adopted over multiple years. The evidence is most dramatic for nonmigratory monophagous and oligophagous species that show high mortality on Bt crops, such as European corn borer (_Ostrinia nubilalis_), and tobacco budworm (_Heliothis virescens_). Bt cotton is currently being used in the southwestern USA as part of an area-wide eradication program for pink bollworm (_Pectinophora gossypiella_). Single, stacked, and...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4447/version/1

Lost in translation: Toward a formal model of multilevel, multiscale medicine
Provedor de dados: 17
Autores: Rodrick Wallace.
For a broad spectrum of low level cognitive regulatory and other biological phenomena, isolation from signal crosstalk between them requires more metabolic free energy than permitting correlation. This allows an evolutionary exaptation leading to dynamic global broadcasts of interacting physiological processes at multiple scales. The argument is similar to the well-studied exaptation of noise to trigger stochastic resonance amplification in physiological subsystems. Not only is the living state characterized by cognition at every scale and level of organization, but by multiple, shifting, tunable, cooperative larger scale broadcasts that link selected subsets of functional modules to address problems. This multilevel dynamical viewpoint has implications...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Immunology; Molecular Cell Biology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6973/version/1

Low Cardiorespiratory Fitness is an Independent Predictor of Metabolic Syndrome in Young Korean Adults
Provedor de dados: 17
Autores: Hyun-Sik Kang; Soo-Hyun Park; Shinuk Kim.
Objective: To investigate the relationship between cardio/respiratory fitness (CRF) and metabolic syndrome (MS) in young Korean men. Design: In a cross-sectional study, we examined 909 young Korean men (mean&amp;#xb1;SD age, 24.0&amp;#xb1;2 years) who were healthy and not taking any medications affecting blood pressure, glucose, or lipids concentrations. Body fatness, resting blood pressures, and fasting blood levels of lipids, glucose, and insulin were measured with our standardized laboratory protocols. CRF was quantified as the maximum volume of minute oxygen consumption measured during a graded treadmill test. Metabolic syndrome was defined with the National Cholesterol Education Program Adult Treatment Panel III criteria and a modified cut-off value...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2236/version/1

Magnetic Resonance imaging (MRI) in detection of _Bifidobacterium longum_ and _Clostridium novyi-NT_ labeled with superparamagnetic iron oxide (SPIO) nanoparticle
Provedor de dados: 17
Autores: Jianhua Wang; Xuebin Zhang; Dayong Zhou; Qingguo Wang; Dan Li.
*Purpose:* To investigate the MR imaging of _Bifidobacterium longum_ and _Clostridium novyi-NT_ labeling with superparamagnetic iron oxide (SPIO) nanoparticles.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;*Materials and methods:* Tubes containing _B. longum_-SPIO, Free-SPIO, _B. longum_ and PYG Medium were incubated under anaerobic condition in _in vitro_ experiment. Transmission electron microscope and Prussian blue staining were used to demonstrate intra-bacteria nanoparticles. R~2~^*^ mapping and R~2~ mapping were reconstructed after MR scanning. _B. longum_-SPIO and _C. novyi_-NT-SPIO were injected respectively _in vivo_ to show whether it might be traced by MR imaging.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;*Results:* Magnetosomes in bacteria were observed by...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Microbiology.
Ano: 2009
URL: http://precedings.nature.com/documents/3435/version/1

Major Forces and Factors Shaping Global Markets: The Emerging Agricultural World
Provedor de dados: 31
Autores: Brookins,   Carole L.
The world is producing greater opportunity for more people than ever before in the history of mankind. The global agri-food system is dynamic, not static. In order to understand the future trends and potentials for utilizing and improving our agri-food system, it is critical to focus on forces that will improve both productivity and policy to meet the demands of the ever-changing global market. This paper identifies five major trends that will impact the global economy and create structural change in commodity markets.
Tipo: Journal Article
Palavras-chave: Biotechnology; Energy; Environment; Farm policy; Food safety; Information technology; Infrastructure gaps; Trade logistics; Agribusiness; Food Consumption/Nutrition/Food Safety.
Ano: 2007
URL: http://purl.umn.edu/62280

MANAGING PEST RESISTANCE: THE POTENTIAL OF CROP ROTATIONS AND SHREDDING
Provedor de dados: 31
Autores: Babcock,   Bruce A.; Secchi,   Silvia.
The current debate over resistance management plans mandated by the Environmental Protection Agency for transgenic crops ignores management practices that are complementary to refuge schemes.  A dynamic production model is developed that measures the costs and benefits of crop rotation and shredding in terms of delaying resistance to Bt corn.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Common property resource; Crop rotation; European Corn Borer; Externalities; Insecticide resistance; Integrated pest management; Intertemporal optimization; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/21597

Manual de biossegurança da Embrapa Soja.
Provedor de dados: 14
Autores: MARCELINO-GUIMARÃES, F. C.; ARIAS, C. A. A.; HUNGRIA, M.
Definição. Tipos de Risco. Riscos de acidentes. Riscos Físicos. Riscos químicos. Riscos biológicos. Classificação de risco biológico. Níveis de contenção física para riscos biológicos. Classificação de risco biológico de organismos geneticamente modificados (OGMs). Níveis de biossegurança para trabalhos com OGMs. Métodos de controle de agentes de risco. Recomendações gerais. Barreiras de proteção. Procedimentos para descarte dos resíduos. Instalações físicas e procedimentos em contenção para atividades e projetos com vegetais geneticamente modificados. Manuseio dos materiais GM. Rotinas de esterilização. Anexo 1 - Procedimento para uso de cabines de biossegurança. Anexo 2 - Legislação referentes às atividades descritas neste Manual.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Biotecnologia; Biossegurança; Organismo transgênico; Biotechnology; Biosecurity.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/998975

Margarine products quality monitoring using reflectance UV-VIS-SWNIR spectroscopy
Provedor de dados: 17
Autores: Rui C. Martins; Vitor V. Lopes; Ant&amp;#xf3;nio A. Vicente.
Margarine is a term that can indicate any of a wide range of butter substitutes. Due to the great diversity of the raw material, margarine end-product quality characteristics are expected to be highly diversified. This article proposes the use of reflectance UV-VIS-SWNIR spectroscopy to monitor the margarine end-product quality properties. The main effort in this work was the development of a fast monitoring procedure to assess the quality of the manufactured margarines. &amp;#xd;&amp;#xa;The study was performed on two margarine products: regular margarine (less than 80% fat) and reduced-fat margarine (less than 60% fat). The nine product samples were collected during the production line normal operating conditions on different days. The samples had the...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2293/version/1

Marked Rise in Replikin Counts in H5N1 Influenza Virus Localized to Lethality Gene p B1.
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Abstract: Virus outbreaks have been found to be related to the concentration of a new class of genomic peptides, Replikins^1^. The eight genes of H5N1 influenza virus were analyzed for the distribution of Replikin Counts (number Replikins /100 amino acids) in 2,441 sequences from birds and humans. An increase (p&amp;#x3c;0.001) occurred from 2004 to August 2011 in one gene, pB1.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Immunology; Microbiology; Molecular Cell Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6420/version/1

Market Impacts of Adopting Herbicide-Resistant Rice in the Southern United States
Provedor de dados: 31
Autores: Fuller,   Frank H.; Annou,   Mamane Malam; Wailes,   Eric J.
Herbicide-resistant (HR) rice varieties offer U.S. rice producers a powerful tool for control of red rice infestations.  However, improved weed control can shorten crop rotations and boost yields, resulting in expanded rice production and lower domestic market prices.  Declining market returns diminish the benefits of HR rice adoption and substantially reduce net returns for nonadopters.  More competitive prices increase U.S. rice exports, causing a slight decline in world rice prices.  The dependence of the rice marketing loan program on world prices prevents loan deficiency payments from adequately offsetting producers’ market revenue losses.  U.S. consumers gain from lower rice prices.
Tipo: Journal Article
Palavras-chave: Biotechnology; Crop rotation; Herbicide resistant; Red rice; Rice; Simulation models; Technology adaptation; Q11; Q16; Q18; O33; C53.
Ano: 2003
URL: http://purl.umn.edu/37859

MARKET STRUCTURE IN BIOTECHNOLOGY: IMPLICATIONS FOR LONG-RUN COMPARATIVE ADVANTAGE
Provedor de dados: 31
Autores: Lavoie,   Brian F.; Sheldon,   Ian M.
A country specializing in a high technology industry may find that excess returns stemming from innovation are reallocated overseas as foreign-based multinationals access ongoing domestic R&amp;D through alliances with or acquisition of established domestic start-ups. Computer simulation illustrates this process in the context of the current US specialization in biotechnology. Keywords: Biotechnology, market structure, comparative advantage
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Market structure; Comparative advantage; Research and Development/Tech Change/Emerging Technologies.
Ano: 2000
URL: http://purl.umn.edu/21835

MARKETING STRATEGIES OF BIOTECHNOLOGY FIRMS: IMPLICATIONS FOR U.S. AGRICULTURE
Provedor de dados: 31
Autores: Renkoski,   Matthew A.
DuPont Quality Grains is focused on improving grain quality for end users, rather than on farm production traits.  A major DuPont program is high oil corn.  Feed corn has a huge market, and, because it requires no intermediate processing, any enhanced value is measurable by end users.  Standard commodity markets do not function for capturing the value of enhance grains, and so DuPont works with end users, elevators, farmers, and seed companies to create market channels.  As biotechnology commercializes more value-enhancing traits targeted to specific agricultural customers, vertical value chains will become shorter and more coordinated while standard commodity markets will diminish.
Tipo: Journal Article
Palavras-chave: Biotechnology; DuPont; Marketing strategies; Research and Development/Tech Change/Emerging Technologies.
Ano: 1997
URL: http://purl.umn.edu/15550

Mass Cultivation of _Chroococcus turgidus_ and _Oscillatoria sp._ and Effective Harvesting of Biomass by Low-cost methods
Provedor de dados: 17
Autores: Mohan N; Hanumantha Rao P; Ranjith Kumar R; Sivasubramanian V.
The potential of microalgal biomasses for the production of biochemicals such as pigments, vitamins, antibiotics, polysaccharides, proteins, essential fatty acids, bio-flocculants, enzymes, etc. is well known. The purpose of this study was to cultivate two cyanobacterial species namely _Chroococcus turgidus_ and _Oscillatoria sp._, isolated from industrial effluents, using a suitable growth medium in a large-scale High Rate Algal (HRA) pond. The biomolecules such as total protein, total carbohydrate and total lipid, and the pigments chlorophyll, phycobilins, &amp;#x3b2;-carotene, were analysed at regular intervals during cultivation. In addition, the total bacterial cell numbers were enumerated during the study and their influence on algal growth was...
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4331/version/1

Medicine beyond magic bullets: a formal case for multilevel interventions
Provedor de dados: 17
Autores: Rodrick Wallace.
Western medicine&amp;#x27;s paradigmatic search for &amp;#x27;magic bullet&amp;#x27; interventions is facing increasing difficulty: Between 1950 and 2010 the inflation-adjusted cost per USFDA-approved drug has increased exponentially in time, a draconian inverse of the famous Moore&amp;#x27;s Law of computing. A sequence of empirically-oriented statistical models suggests that carefully designed synergistic multifactorial and multiscale strategies might evade this relationship.
Tipo: Manuscript
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6780/version/1

Melhoramento da manga (Mangifera indica L.) no Brasil: sinopse de projeto.
Provedor de dados: 14
Autores: PINTO, A. C. de Q.; ANJOS, J. R. N. dos; ANDRADE, S. R. M.; FALEIRO, F. G.; CORDEIRO, M. C. R.; DIAS, J. N.
bitstream/CPAC-2009/24316/1/p2003_05.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Melhoramento genetico; Mango; Tropical fruits.; Biotecnologia; Fruta Tropical; Mangifera Indica.; Manga; Biotechnology; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567612

Metabolic, Replication and Genomic  Category of Systems in Biology,  Bioinformatics and Medicine
Provedor de dados: 17
Autores: I. C. Baianu.
Metabolic-repair models, or (M,R)-systems were introduced in Relational Biology by Robert Rosen. Subsequently, Rosen represented such (M,R)-systems (or simply MRs)in terms of categories of sets, deliberately selected without any structure other than the discrete topology of sets. Theoreticians of life&amp;#x2019;s origins postulated that Life on Earth has begun with the simplest possible organism, called the primordial. Mathematicians interested in biology attempted to answer this important question of the minimal living organism by defining the functional relations that would have made life possible in such a minimal system- a grandad and grandma of all living organisms on Earth. Genomic systems are also considered as molecular realizations of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Molecular Cell Biology; Neuroscience; Bioinformatics; Earth &amp; Environment; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7051/version/1

Methanogen consortium metabolism characteristic analysis of Illinois basin by real time monitor method
Provedor de dados: 17
Autores: Dong Xiao; Enyuan Wang.
Microbially Enhance Coalbed Methane technology is used to improve methane content in coalbeds. The methanogen consortium metabolism process is the basic avenue for MECoM to yield methane with some organic compounds in coal. Detailed information about the metabolism process is needed in studying the microbial consortium mechanism. Traditional gas analysis is limited by gas source and test complexity. The real time monitor with Infrared gas sensor breaks the gas analysis limitation. It shows much more detailed information than the traditional analysis method including information about microbial growth factor changes, reaction speeds for environmental changes, the detailed relationship between CH4 and CO2, methane yield and the consumption factor by real...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2010
URL: http://precedings.nature.com/documents/5426/version/1

Metodologia para operacionalizar a extracao de DNA de especies nativas do cerrado visando a analises moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.
bitstream/CPAC-2009/24559/1/comtec_92.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Biotecnologia; Biologia Molecular; Cerrado; Engenharia Genética; DNA; Marcador Genético; Marcador Molecular; Melhoramento Genético Vegetal.; Biotechnology; Genetic engineering; Genetic markers; Plant breeding.; Molecular biology.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/559088

Microalgues marines : les enjeux de la recherche
Provedor de dados: 5
Autores: Muller-feuga, Arnaud.
Industrial potentialities related to biotechnology of microalgae are examined, highlighting a wide spectrum of applications which concern principally nutrition, health, environment and energy. The french situation of research on microalgae, especially at Ifremer, is assessed, showing that the main axis are fine chemistry, aquaculture, environment, bio-process engineering. It covers the majority of the applications, but leaves in shade certain important aspects such as human food and CO2 fixation. Concerning the general approach, some significant improvements can be expected from a more accurate control of bacteriological and genetic quality of cultures. About the industrial aspects, a national initiative seems in progress. The variety of potential...
Tipo: Text
Palavras-chave: Process engineering; Energy; Environment; Health; Food; Aquaculture; Biotechnology; Microalgae; Génie des procédés; Energie; Environnement; Santé; Alimentation; Aquaculture; Biotechnologie; Microalgues.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/rapport-1092.pdf

Microbial diversity, metagenomics and the Yucatán aquifer
Provedor de dados: 85
Autores: Rojas-Herrera,R; Zamudio-Maya,M; Arena-Ortiz,L; Pless,RC; O'Connor-Sánchez,A.
Mexico counts among the five countries with the highest biodiversity in the world. In the Yucatán Peninsula, there are aquatic ecosystems with a very special microbial diversity. These ecosystems are essential for the ecological equilibrium of the region, and are seriously threatened by human activities. Access and knowledge of the microbial resources of these environments have an enormous scientific interest, and could potentially result in biotechnological products which could lead to more efficient and environmentally friendly processes; it could also offer a full arsenal of microorganisms and/or novel molecules to the local and world industry to face the current needs. In recent years, with the rise of metagenomic technology, knowledge about microbial...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Ecosystems; Biotechnology; 454-sequencing.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572011000200017

Micro-magnetic resonance imaging (micro-MRI) study on the sepsis effected eyeball of zebrafish
Provedor de dados: 17
Autores: Bimal Kumar  Sarkar; Wu  Zen; Shangwu  Ding; C. H.  Hsu; Z.H.  Wen; C. S.  Lin; Chiranjib  Chakraborty.
We have studied the normal and sepsis effected zebrafish eyeball with micro-magnetic resonance imaging (micro-MRI). T2 weighted image was studied and pixel-wise T2 maps were co registered among the normal and sepsis effected eye. From the micro-image the sepsis effect in the eye has been demonstrated. The pixel-wise brightness distribution is not so scattered in the normal eyeball image, whereas it is very scattered in case of sepsis affected eyeball image. Also the T2 mapping on the eye has given valuable information that would be a potential tool for the study of diseased organ in the micro level. From T2 mapping, it has shown that the T2 in normal eyeball have low values in comparison to the sepsis affected eyeball.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2694/version/1

Migraine aura: retracting particle-like waves in weakly susceptible cortex
Provedor de dados: 17
Autores: Markus A. Dahlem; Nouchine Hadjikhani.
Cortical spreading depression (SD) has been suggested to underlie migraine aura. Despite a precise match in speed, the spatio-temporal patterns of SD and aura symptoms on the cortical surface ordinarily differ in aspects of size and shape. We show that this mismatch is reconciled by utilizing that both pattern types bifurcate from an instability point of generic reaction-diffusion models. To classify these spatio-temporal pattern we suggest a susceptibility scale having the value [sigma]=1 at the instability point. We predict that human cortex is only weakly susceptible to SD ([sigma]&amp;#x3c;1), and support this prediction by directly matching visual aura symptoms with anatomical landmarks using fMRI retinotopic mapping. We discuss the increased...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2070/version/1

Milk is for Children, Colostrum silage is for calves.
Provedor de dados: 17
Autores: Mara Helena Saalfeld; Daniela  Brayer Pereira; Kathleen Kr&amp;#xfc;guer Silveira; Marcia Gularte; Julia Valente; Renata Schramm; F&amp;#xe1;bio Leivas Leite.
One sixth of the world population is starving. In the meantime, producers from all over the world daily disdain billions of liters of bovine colostrum, which is seen as rich in nutrients, immunoglobulin and bioactive substances. The milk is the most expensive component in the final costs of calves breeding. Considering the impossibility of substituting the milk to feed the calf, different ways to use the colostrum have been studied however with controversial results. We have developed colostrum silage. This product is economical and possible to store in the environment for up to eighteen months. Being efficient for calf breeding, yielding income and profit to the dairy business. The colostrum silage keeps the necessary physicochemical characteristics for...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6553/version/1

MIREOT: the Minimum Information to Reference an External Ontology Term
Provedor de dados: 17
Autores: Melanie Courtot; Frank Gibson; Allyson L. Lister; James Malone; Daniel Schober; Ryan R. Brinkman; Alan Ruttenberg.
While the Web Ontology Language (OWL) provides a mechanism to import ontologies, this mechanism is not always suitable. First, given the current state of editing tools and the issues they have working with large ontologies, direct OWL imports have sometimes proven impractical for day-to-day development. Second, ontologies chosen for integration may be under active development and not aligned with the chosen design principles. Importing heterogeneous ontologies in their entirety may lead to inconsistencies or unintended inferences. In this paper we propose a set of guidelines for importing required terms from an external resource into a target ontology. We describe the guidelines, their implementation, present some examples of application, and outline...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2009
URL: http://precedings.nature.com/documents/3574/version/1

MIREOT: the Minimum Information to Reference an External Ontology Term
Provedor de dados: 17
Autores: M&amp;#xe9;lanie Courtot; Frank Gibson; Allyson L. Lister; James Malone; Daniel Schober; Ryan R. Brinkman; Alan Ruttenberg.
While the Web Ontology Language (OWL) provides a mechanism to import ontologies, this mechanism is not always suitable. First, given the current state of editing tools and the issues they have working with large ontologies, direct OWL imports have sometimes proven impractical for day-to-day development. Second, ontologies chosen for integration may be under active development and not aligned with the chosen design principles. Importing heterogeneous ontologies in their entirety may lead to inconsistencies or unintended inferences. In this paper we propose a set of guidelines for importing required terms from an external resource into a target ontology. We describe the guidelines, their implementation, present some examples of application, and outline...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3576/version/1

MMsPred: a bioactivity and toxicology predictive system
Provedor de dados: 17
Autores: Joel Masciocchi; Luca Pireddu; Ricardo Medda; Piergiorgio Palla; Matteo Floris; Luca Pani; Patricia Rodriguez-Tome.
In the last decade, the development and use of new methods in combinatorial chemistry and high-throughput screening has dramatically increased the number of known biologically active compounds. Paradoxically, the number of drugs reaching the market has not followed the same trend, often because many of the candidate drugs present poor qualities in absorption, distribution, metabolism, excretion, and toxicological properties (ADME-Tox). The ability to recognize and discard bad candidates early in the drug discovery steps would save lost investments in time and money. Machine learning techniques could provide solutions to this problem.&amp;#xd;&amp;#xa;The goal of my research is to develop classifiers that accurately discriminate between active and inactive...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4188/version/1

Mobile Image Ratiometry: A New Method for Instantaneous Analysis of Rapid Test Strips
Provedor de dados: 17
Autores: Donald  C. Cooper; Bryan Callahan; Phil  Callahan; Lee  Burnett.
Here we describe Mobile Image Ratiometry (MIR), a new method for the automated quantification of standardized rapid immunoassay strips using consumer-based mobile smartphone and tablet cameras. To demonstrate MIR we developed a standardized method using rapid immunotest strips directed against cocaine (COC) and its major metabolite, benzoylecgonine (BE). We performed image analysis of three brands of commercially available dye-conjugated anti-COC/BE antibody test strips in response to three different series of cocaine concentrations ranging from 0.1 to 300 ng/ml and BE concentrations ranging from 0.003 to 0.1 ng/ml. These data were then used to create standard curves to allow quantification of COC/BE in biological samples. MIR quantification of COC and BE...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6827/version/1

Mobile Image Ratiometry for the Detection of Botrytis cinerea (Gray Mold)
Provedor de dados: 17
Autores: Donald  C. Cooper.
Mobile Platform Informatics (MPI) and Smartphone Informatics (SPI) methods like Mobile Image Ratiometry (MIR) are potentially transformative point-of-use instantaneous analysis tools that are useful across a variety of industries. In agriculture, MIR-compatible immuno test strips allow early detection of a number of biotic stressors before devastating crop losses occur. Here we describe a low-cost and easy-to-use Smartphone and/or tablet-based protocol (Mobile Assay Inc., www.mobileassay.com) for the detection and on-sight instantaneous analysis of B. cinerea, a fungus that causes significant damage to a variety of plants and flowers. Early detection and tracking of the B. cinerea fungus before the visible gray mold appears has the potential to increase...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth &amp; Environment; Plant Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6989/version/1

Modelling Hepatic Endoderm Development: Highly Efficient Differentiation of Human Embryonic Stem Cells to Functional Hepatic Endoderm Requires ActivinA and Wnt3a Signalling.
Provedor de dados: 17
Autores: David Hay; Judy Fletcher; Catherine Payne; John D. Terrace; Ronald C. J. Gallagher; Jan Snoeys; Jim Black; Davina Wojtacha; Kay Samuel; Zara Hannoun; Anne Pryde; Celine Filippi; Ian S. Currie; Stuart J. Forbes; James A. Ross; Philip Newsome; John Iredale.
Human embryonic stem cells (hESCs) are a valuable source of pluripotential primary cells. However, their homogeneous cellular differentiation to specific cell types _in vitro_ has proven difficult thus far. Wnt signalling has been shown to play important roles in coordinating development and we demonstrate that Wnt3a is differentially expressed at critical stages of human liver development _in vivo_. The essential role of Wnt3a in hepatocyte differentiation from hESCs is paralleled by our _in vitro_ model, demonstrating the importance of a physiological approach to cellular differentiation. Our studies provide compelling evidence that Wnt3a signaling is important for coordinated hepato-cellular function _in vitro_ and _in vivo_. In addition, we demonstrate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/1454/version/1

Modelling Hepatic Endoderm Development: Highly Efficient Differentiation of Human Embryonic Stem Cells to Functional Hepatic Endoderm Requires ActivinA and Wnt3a Signalling.
Provedor de dados: 17
Autores: David C. Hay; Judy Fletcher; Catherine Payne; John D. Terrace; Ronald C. J. Gallagher; Jan Snoeys; Jim Black; Davina Wojtacha; Kay Samuel; Zara Hannoun; Anne Pryde; Celine Filippi; Ian S. Currie; Stuart J. Forbes; James A. Ross; Philip Newsome; John P. Iredale.
Human embryonic stem cells (hESCs) are a valuable source of pluripotential primary cells. However, their homogeneous cellular differentiation to specific cell types _in vitro_ has proven difficult thus far. Wnt signalling has been shown to play important roles in coordinating development and we demonstrate that Wnt3a is differentially expressed at critical stages of human liver development _in vivo_. The essential role of Wnt3a in hepatocyte differentiation from hESCs is paralleled by our _in vitro_ model, demonstrating the importance of a physiological approach to cellular differentiation. Our studies provide compelling evidence that Wnt3a signaling is important for coordinated hepato-cellular function _in vitro_ and _in vivo_. In addition, we demonstrate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1454/version/2

Modulação da expressão gênica em plantas via tecnologia CRISPR/dCas9.
Provedor de dados: 14
Autores: MORGANTE, C. V.; ARRAES, F. B. M.; PINTO, C. E. M.; MELO, B. P. de; GROSSI-DE-SA, M. F.
Este capítulo aborda a tecnologia CRISPR pelo uso da variante dead Cas9 (CRISPR/ dCas9), com ênfase no seu uso para a modulação da expressão gênica em plantas. São apresentadas e discutidas as principais estratégias atualmente utilizadas, para as quais foi proposto um roteiro, considerando os principais aspectos para um bom desenho experimental.
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: CRISPR; Genoma da planta; Expressão gênica.; Biotecnologia; DNA; Genoma; Genética.; Biotechnology; Plant genetics.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1126520

Molecular Bio-imprinting of Biocatalysts
Provedor de dados: 17
Autores: Israr  Khan; Muhammad Waheed Akhtar.
Energy conservation is the cry of the day. Attempts are made all over the world to occupy and use energy reserves. Increased industrialization and mechanization has led to the  depletion of natural energy reserves. Its unavoidable to search for renewable sources of energy, which may be not used now but can be used by future generations. We are using the expertise of our ancestors. Thus exploiting the nature and newer techniques in this area would yield the best results. Bio-imprinting is one of those techniques whereby chemical modification is done in order to achieve highly expressed protein which can be stored in its highly active form in the specific solvent.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Ecology; Genetics &amp; Genomics; Microbiology; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics; Earth &amp; Environment; Plant Biology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5665/version/1

Molecular crowding defines a common origin for the Warburg effect in proliferating cells and the lactate threshold in muscle physiology
Provedor de dados: 17
Autores: Alexei Vazquez.
Aerobic glycolysis is a seemingly wasteful mode of ATP production that is seen both in rapidly proliferating mammalian cells and highly active contracting muscles, but whether there is a common origin for its presence in these widely different systems is unknown. To study this issue, here we develop a model of human central metabolism that incorporates a solvent capacity constraint of metabolic enzymes and mitochondria, accounting for their occupied volume densities, while assuming glucose and/or fatty acid utilization. The model demonstrates that activation of aerobic glycolysis is favored above a threshold metabolic rate in both rapidly proliferating cells and heavily contracting muscles, because it provides higher ATP yield per volume density than...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5784/version/1

Molecular Models of DNA Structures and Dynamics: Principles, Techniques and Applications
Provedor de dados: 17
Autores: I. C. Baianu.
Molecular models of DNA structures are representations of the molecular geometry and topology of Deoxyribonucleic acid (DNA) molecules using one of several means, such as: closely packed spheres (CPK models) made of plastic, metal wires for &amp;#x27;skeletal models&amp;#x27;, graphic computations and animations by computers, artistic rendering, and so on, with the aim of simplifying and presenting the essential, physical and chemical, properties of DNA molecular structures either in vivo or in vitro. Computer molecular models also allow animations and molecular dynamics simulations that are very important for understanding how DNA functions in vivo.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Microbiology; Molecular Cell Biology; Bioinformatics; Plant Biology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7075/version/1

Molecular Static and Dynamic Analyses reveal Flaw in Murine Model used by US FDA to Detect Drug Carcinogenicity
Provedor de dados: 17
Autores: Trevor Marshall.
The US FDA currently accepts carcinogenicity studies of pharmaceutical drugs based on murine models. In addition to 6 month studies with p53(+/-) and ras.H2 transgenic mice, lifetime studies (typically 2 years) in WT mice or rats are also considered as evidence that a drug lacks carcinogenic activity. This model is not always exhaustive. For example, during the acceptance testing of the ARB Olmesartan[1], possible carcinogenicity observed in hamsters was not able to be duplicated in rats, or in transgenic mice. We have previously used the static molecular modeling of AutoDock to demonstrate that Olmesartan has agonostic activity in the PDB:1DB1 model of the human VDR Nuclear Receptor[2], while it has antagonistic activity in the PDB:1RK3 model of the rat...
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Immunology; Pharmacology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/52/version/1

MRI and MRSI Application in Neurodegeneration
Provedor de dados: 17
Autores: Rakesh Sharma.
MRI combined with MR spectroscopy generates better information of both brain tissue composition and mesurement of metabolites or neurochemicals in multiple sclerosis(MS), Alzheimer&amp;#x27;s disease and epilepsy. New fast imaging techniques are technical advance in visualizing accurate anatomy and measurement of absolute neurochemicals in brain. Gray metter seated MS lesions is a new form of disease and lesion may appear normal on MRI but show spectroscopy peaks. High magnetic field scanners are available for fast imaging.
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4317/version/1

Multi-Cancer Computational Analysis Reveals Metastasis-Associated Variant of Desmoplastic Reaction Involving INHBA and THBS2
Provedor de dados: 17
Autores: Hoon Kim; John Watkinson; Vinay Varadan; Dimitris Anastassiou.
Despite extensive research, the details of the metastasis-associated biological mechanisms are largely unknown. Here, we analyze data from multiple cancers using a novel computational method identifying sets of genes whose coordinated overexpression indicates the presence of a particular phenotype. We conclude that there is one shared &amp;#x201c;core&amp;#x201d; metastasis-associated gene expression signature corresponding to a specific variant of desmoplastic reaction, present in a large subset of samples that have exceeded a threshold of invasive transition specific to each cancer, indicating that the biological mechanism is triggered at that point. For example this threshold is reached at stage IIIc in ovarian cancer and at stage II in colorectal...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4503/version/1

Multifunction moonlighting and intrinsically disordered proteins: Information catalysis, nonrigid molecule symmetries, and the &amp;#x27;logic gate&amp;#x27; spectrum
Provedor de dados: 17
Autores: Rodrick Wallace.
Intrinsically disordered proteins (IDP) appear far more likely to engage in functional moonlighting than well-structured proteins. The recent use of nonrigid molecule theory to address IDP structure and dynamics produces this result directly: Mirror image subgroup or subgroupoid tiling matching of the molecular fuzzy lock-and-key can be much richer for IDPs since the number of possible group or groupoid symmetries can grow exponentially with molecule length, while tiling matching for 3D structured proteins is relatively limited. A simple information catalysis model suggests how this mechanism can produce a vast spectrum of biological &amp;#x27;logic gates&amp;#x27; having subtle properties far beyond familiar AND, OR, XOR, etc. behaviors. Inferring the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Molecular Cell Biology; Pharmacology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6413/version/1

Multifunctional Hybrid Silica Particles for Multicolor Imaging and Multiplex Tasking
Provedor de dados: 17
Autores: Sang Man koo; Hae Sung Kim; Chan Yoon Jung; Ha Young Kim; Hoe Jin Hah.
Multifunctional submicron particles have recently emerged as great promises for biological applications such as bio-labeling, medical diagnostics, and drug delivery. These particles are non-toxic and have size comparable to biomolecules, becoming very suitable for biomedical applications. The particle structures can be engineered to perform multicolor imaging, as well as multiplex tasking such as site-selective binding, detection, and separation. In addition, these particles can load a large amount of fluorescent dye for signal amplification. Several synthetic techniques have been developed for a variety of submicron particles, including core-shell synthesis, layer-by-layer techniques, multi-block polymer emulsifications, and surface modifications. Silica...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/1621/version/1

Multifunctional Nanoparticles for Imaging Guided Interventions
Provedor de dados: 17
Autores: Yousef Haik; Basel al-Ramadi; Bashar Issa; Shahnaz Qadri; Saleh Hayek; Hassan Hijaze.
We describe multifunctional magnetic nanoparticles (MNPs) encapsulated in thermosensitive, drug-bearing shells and delivered to the tumor site by genetically modified and non-pathogenic strains of bacteria with known affinity to tumors for an effective and minimally invasive protocol for tumor management. The magnetic nanoparticles also serve as a non-invasive imaging contrast agent, heating agent as well as thermometry monitoring agents. We have shown an efficient tumor management on a mouse model utilizing the MNPs. Our studies showed that these novel MNPs significantly reduce the progress of tumor and prolong the animal life and function as an imaging contrast to visually monitor the tumor treatment and evolution.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer.
Ano: 2008
URL: http://precedings.nature.com/documents/2190/version/1

Multi-scale network biology model &amp;#x26; the model library
Provedor de dados: 17
Autores: Jianghui Xiong.
Multi-scale network biology model &amp;#x26; the model library
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5778/version/1

Multivalent ligands of CD22 for targeting of B cells
Provedor de dados: 17
Autores: James C. Paulson.
Multivalent ligands of CD22 for active targeting of B cells. Mary O&amp;#x2019;Reilly, Wei Hsu Chen, Gladys Completo, Ying Zeng, Satoshi Futakawa and Cory Rillahan and James C. Paulson, Departments of Chemical Physiology and Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA, 92037&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The siglecs comprise 13 members of the immunoglobulin superfamily that recognize sialic acid containing glycans, and are differentially expressed on leukocytes and glial cells.   The natural ligands of siglecs typically occur on the same cell (in cis) and/or on adjacent cells (in trans).  Cis ligands mask the binding of multivalent synthetic sialoside ligands and are thought to regulate the activity of...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry; Immunology; Molecular Cell Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3549/version/1

Nanoconfinement of spider silk fibrils begets superior strength, extensibility and toughness
Provedor de dados: 17
Autores: Tristan Giesa; Melis Arslan; Nicola Pugno; Markus J. Buehler.
Silk is an exceptionally strong, extensible and tough material made from simple protein building blocks. The molecular structure of dragline spider silk repeat units consists of semi-amorphous and nanocrystalline beta-sheet protein domains. Here we show by a series of computational experiments how the nanoscale properties of silk repeat units are scaled up to create macroscopic silk fibers with outstanding mechanical properties despite the presence of cavities, tears and cracks. We demonstrate that the geometric confinement of silk fibrils to diameters of 50&amp;#xb1;30 nm width is critical to facilitate a powerful mechanism by which hundreds of thousands of protein domains synergistically resist deformation and failure to provide enhanced strength,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics; Earth &amp; Environment.
Ano: 2011
URL: http://precedings.nature.com/documents/5916/version/2

Nano-enabled Biological Materials
Provedor de dados: 17
Autores: Bradly J. Alicea.
This talk is an attempt to define a new field called &amp;#x201c;Nano-enabled Biological Tissues&amp;#x201d;. As such, this talk serves as a review of both the theoretical underpinnings and relevant recent results. &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The presentation is divided into several parts: &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;* in the first section (slides 3-4), the concept of nano-enabled tissues are introduced as a complex system that can be engineered at multiple scales.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;* the second section (slides 5-12) contains three essential ingredients to achieve the technological vision. Current examples of each ingredient are introduced separately.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;* in the third section (slides 13-17),...
Tipo: Presentation
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5448/version/1

Nanofiber fabrication in a temperature and humidity controlled environment for improved fibre consistency
Provedor de dados: 17
Autores: Oliver Hardick; Bob Stevens; Daniel G.  Bracewell.
To fabricate nanofibers with reproducible characteristics, an important demand for many applications, the effect of controlled atmospheric conditions on resulting electrospun cellulose acetate (CA) nanofibers was evaluated for temperature ranging 17.5 - 35&amp;#xb0;C and relative humidity ranging 20% - 70%. With the potential application of nanofibers in many industries, especially membrane and filter fabrication, their reproducible production must be established to ensure commercially viability.&amp;#xd;&amp;#xa;Cellulose acetate (CA) solution (0.2 g/ml) in a solvent mixture of acetone/DMF/ethanol (2:2:1) was electrospun into nonwoven fibre mesh with the fibre diameter ranging from 150nm to 1&amp;#xb5;m.&amp;#xd;&amp;#xa;The resulting nanofibers were...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4524/version/1

Nanotoxicity Assessment toward the Applications of Carbon Nanotubes as a Small Biomolecule Carrier in Drug Delivery Systems
Provedor de dados: 17
Autores: Rakesh  Sharma; Soonjo  Kwon; R Baktur; Avdhesh Sharma.
Carbon Nanotube (CNT) materials have superior properties in electric current carrying capacity, thermal conductivity, and thermal stability. Due to their structure with high aspect ratio, they have structural unusual toxicity and complicate safety issue in a target tissue.  In optimized quantities with limited functionality, special type of CNT assembly such as &amp;#x201c;buckyball&amp;#x201d; can be used as a potential drug carrier of bioactive molecules and display with increased circulating time and acceptable functionality.  We analyzed cytoxicity and inflammatory response following exposure of different size, mass, shape, and functionality of CNTs. We monitored the transport across skin, physiological perturbation of transepithelial electrical...
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3948/version/1

Narrow genetic and apparent phenetic diversity in Jatropha curcas: initial success with generating low phorbol ester interspecific hybrids
Provedor de dados: 17
Autores: Siam  Popluechai; Diego Breviario; Sujatha Mulpuri; Harinder P. S. Makkar; Manish Raorane; Attipalli R. Reddy; Enrico Palchetti; Angharad M. R. Gatehouse; Keith J. Syers; Anthony G. O&amp;#x27;Donnell; Ajay Kohli.
Due to the increasing popularity of Jatropha curcas as a feedstock for biodiesel, generating non-toxic and high yielding varieties of the plant requires genotypic characterization towards identifying breeding lines. There is little information on the phylogenetic relationships between its global accessions and species. Assessing genetic variation by RAPD, AFLP and combinatorial tubulin based polymorphism (cTBP) in 38 J. curcas accessions from 13 countries on 3 continents revealed narrow genetic diversity. However, 6 different species of Jatropha from India exhibited pronounced genetic diversity indicating possibilities of improving J. curcas by interspecific breeding. The relatively unexplored cTBP approach we used was a highly efficient and cost effective...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Earth &amp; Environment; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/2782/version/1

National Agricultural Biotechnology Research Capacity in Developing Countries
Provedor de dados: 31
Autores: Cohen,   Joel I.; Komen,   John; Zepeda,   Jose Falck.
Adequate public research capacity is key to the appropriate development of biotechnology, including genetically modified (GM) crops. While commercial crops can be introduced without intensive local research (i.e. insect resistant GM cotton), introducing products of public research depend on indigenous capacity. This paper defines capacity for agricultural biotechnology research and then provides national funding levels for such work in six developing countries. As one indicator of capacity and outputs, GM crops developed from public research in developing countries are documented, and attention given to issues remaining for capacity, research and development. Knowledge of investments in public biotechnology improves policy decisions, clarifies roles of the...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Research Capacity; GMO; Biosafety; Investments; Funding.; Research and Development/Tech Change/Emerging Technologies; O31; O38; Q16.
Ano: 2004
URL: http://purl.umn.edu/23790

Near Infrared Microspectroscopy, Fluorescence Microspectroscopy, Infrared Chemical Imaging and  High-Resolution Nuclear Magnetic Resonance Analysis of Soybean Seeds, Embryos and Single Cells
Provedor de dados: 17
Autores: I. C. Baianu; Schyler S.  Korban; Tiefeng You.
Chemical analysis of soybean seeds, somatic embryos and single cells were carried out by Fourier Transform Infrared (FT-IR), Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fluorescence and High-Resolution NMR (HR-NMR). The first FT-NIR chemical images of biological systems approaching 1 micron (1&amp;#x3bc;) resolution are presented here. Chemical images obtained by FT-NIR and FT-IR Microspectroscopy are presented for oil in soybean seeds and somatic embryos under physiological conditions. FT-NIR spectra of oil and proteins were obtained for volumes as small as 2&amp;#x3bc;3. Related, HR-NMR analyses of oil contents in somatic embryos are also presented here with nanoliter precision. Such 400 MHz 1H NMR analyses allowed the selection of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6200/version/1

Near Infrared Reflectance Spectroscopy (NIRS) Determination of Isoflavone Contents for Selected Soybean Accessions
Provedor de dados: 17
Autores: I. C. Baianu; Jun  Guo; Tiefeng  You; Randall   L. Nelson.
Soybean isoflavones are of considerable interest in relation to their possible health effects in human diets. The rapid and economical determination of soybean isoflavone concentrations is essential for the investigation and development of soybean health foods as well as the selection of soybean seeds with optimal isoflavone levels for such foods. Fourier transforms near infrared reflectance spectroscopy (FT-NIRS) calibrations were developed for the rapid and cost-effective analysis of isoflavones in soybean seeds. FT-NIRS measurements were carried out in quadruplicate for 50 soybean lines selected from the USDA Soybean Germplasm Collection. The selected soybean seeds provided a wide range of isoflavone concentrations (from 0.3 to 6.0 mg/g) that is...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Molecular Cell Biology; Plant Biology; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7048/version/1

Networks of intergenic long-range enhancers and snpRNAs drive castration-resistant phenotype of prostate cancer and contribute to pathogenesis of multiple common human disorders
Provedor de dados: 17
Autores: Anna Glinskii; Shuang Ma; Jun Ma; Denise Grant; Chang-Uk  Lim; Ian Guest; Stewart Sell; Ralph Buttyan; Gennadi Glinsky.
Biological and mechanistic relevance of intergenic disease-associated genetic loci (IDAGL) containing highly statistically significant disease-linked SNPs remains unknown. Here we present the experimental and clinical evidence revealing important role of IDAGL in human diseases. Targeted RT-PCR screen coupled with sequencing of purified PCR products detects widespread transcription at multiple intergenic disease-associated genomic loci (IDAGL) and identifies 96 small non-coding trans-regulatory RNAs of ~ 100-300 nt in length containing SNPs associated with 21 common human disorders (snpRNAs). Functionality of snpRNAs is supported by multiple independent lines of experimental evidence demonstrating their cell-type-specific expression and evolutionary...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6069/version/1

Neural Networks, Cell Genome and Interactome Nonlinear Dynamic Models
Provedor de dados: 17
Autores: I. C. Baianu.
Operational logic and bioinformatics models of nonlinear dynamics in complex functional systems such as neural networks, genomes and cell interactomes are proposed. &amp;#x141;ukasiewicz Algebraic Logic models of genetic networks and signaling pathways in cells are formulated in terms of nonlinear dynamic systems with n-state components that allow for the generalization of previous logical models of both genetic activities and neural networks. An algebraic formulation of variable &amp;#x27;next-state functions&amp;#x27; is extended to a &amp;#x141;ukasiewicz Topos with an n-valued &amp;#x141;ukasiewicz Algebraic Logic subobject classifier description that represents non-random and nonlinear network activities as well as their transformations in...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6206/version/1

New fungal sources for &amp;#x3b1;-L-Rhamnosidase: an important enzyme used in the synthesis of drugs and drug precursors
Provedor de dados: 17
Autores: Vinita  Yadav; Kapil Deo Singh Yadav.
Two fungal strains were isolated and tentatively identified as Penicillium VY and Aspergillus VY. All the isolated species show the maximum production on third day in a liquid culture media. The pH optimum was found to be 10.0 for Penicillium VY and 11.0 for Aspergillus VY. The temperature optima were 50&amp;#xba;C in both the cases. The enzyme produced by Penicillium VY was found to be stable in the pH range 3.0-7.0 and 3.0&amp;#x2013;6.0 in case of Aspergillus VY. The enzyme does not loose activity up to 40&amp;#xba; C in case of Penicillium VY and 40&amp;#xba;C in case of Aspergillus VY if exposed for 1 h.&amp;#xd;&amp;#xa;
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/2560/version/1

Newer Methods of Nanoparticle Synthesis: Nitroimidazole properties with Nanometal oxides in Polymer Cages as Drug-Biomarker Monitors
Provedor de dados: 17
Autores: Rakesh Sharma.
Nitroimidazoles are radiosensitizers and hypoxia detecting chemosensitizers. Intially nitroimidazoles were single dose antibiotic drugs. Recently, nitroimidazole derivatives have emerged as multifunctional &amp;#x201c;drug-biomarker monitors&amp;#x201d; chemical compounds with importance in treatment of tumors, monitoring hypoxia and safer imaging contrast agents to monitor the therapeutic progress.  Additionally, the number of nitroimidazole derivatives is growing and identified their importance as multifunctional nanoparticles. The nanoparticle synthesis scheme of nitroimidazole was proposed as nitroimidazole caged with paramagnetic metal oxide inside polymer coating and labeled with marker radiolebels or biomolecules. We report the step by step...
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3952/version/1

NIR Calibrations for Soybean Seeds and Soy Food Composition Analysis: Total Carbohydrates, Oil, Proteins and Water Contents
Provedor de dados: 17
Autores: I. C. Baianu; Jun Guo.
Conventional chemical analysis techniques are expensive, time consuming, and often destructive. The non-invasive Near Infrared (NIR) technology was introduced over the last decades for wide-scale, inexpensive chemical analysis of food and crop seed composition (see Williams and Norris, 1987; Wilcox and Cavins, 1995; Buning and Diller, 2000 for reviews of the NIR technique development stage prior to 1998, when Diode Arrays were introduced to NIR). NIR spectroscopic measurements obey Lambert and Beer&amp;#x2019;s law, and quantitative measurements can be successfully made with high speed and ease of operation. NIR has been used in a great variety of food applications. General applications of products analyzed come from all sectors of the food industry...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6611/version/1

NIR Calibrations for Soybean Seeds and Soy Food Composition Analysis: Total Carbohydrates, Oil, Proteins and Water Contents [v.2]
Provedor de dados: 17
Autores: I. C. Baianu; Jun Guo; Randall L. Nelson; Tiefeng You; Doina M. Costescu.
Conventional chemical analysis techniques are expensive, time consuming, and often destructive. The non-invasive Near Infrared (NIR) technology was introduced over the last decades for wide-scale, inexpensive chemical analysis of food and crop seed composition (see Williams and Norris, 1987; Wilcox and Cavins, 1995; Buning and Diller, 2000 for reviews of the NIR technique development stage prior to 1998, when Diode Arrays were introduced to NIR). NIR spectroscopic measurements obey Lambert and Beer&amp;#x2019;s law, and quantitative measurements can be successfully made with high speed and ease of operation. NIR has been used in a great variety of food applications. General applications of products analyzed come from all sectors of the food industry...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6611/version/2

NIR Spectroscopy and Microspectroscopy Analysis of Intact Soybean Seeds for Food Applications through Composition Improvements. Version 2.0
Provedor de dados: 17
Autores: Tiefeng You; I. C. Baianu.
The soybean-derived products are among the most important agricultural products in the USA and the world. Conventional analytical methods for soybean composition analysis are both time consuming and costly. Faster and less expensive methods are required for most practical applications. To improve the accuracy, reliability and sensitivity of NIR, major advancements in instrumentation, as well as, data analysis / calibration methodology are required. Novel NIR instruments, such as DA-NIR and FT-NIR spectrometers developed in recent years have the potential for improving significantly the quantification of soybean composition at a reasonable cost. We present representative calibrations and data for intact soybean composition analysis obtained at the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6253/version/1

NMR and Electron Microscopy Studies of Soybean Seeds, Soybean Proteins, and Oil for Improved Utilization in Foods and Feeds
Provedor de dados: 17
Autores: Doina M. Costescu; I. C. Baianu.
NMR, NIR and EM techniques are suitable methods of measuring protein and oil content in soybeans. After completion of a measurement with NMR or NIR, a soybean sample could be either replanted or consummated. NMR techniques involved in the present work are based on the response of nuclei, as nuclear spin flips, when set in a 7.05T magnetic field and excited with rf pulses of 300 MHz. NIR spectroscopy is based on outer electron molecular vibrations, when excited with radiation of frequency 1012 -4&amp;#xd7;1014Hz, and &amp;#xf06c;=2.5Om-750 nm. &amp;#xd;&amp;#xa;TEM and ESEM techniques are bulk and surface analysis techniques, respectively, working with electron beams of 15KV, and image resolution in the range of nm&amp;#x2019;s. ESEM is based on the...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6192/version/1

Nonlinear Models of Neural and Genetic Network Dynamics
Provedor de dados: 17
Autores: I. C. Baianu.
A categorical and &amp;#x141;ukasiewicz-Topos framework for &amp;#x141;ukasiewicz LM-Algebraic Logic models of nonlinear dynamics in complex functional systems such as neural networks, genomes and cell interactomes is proposed. &amp;#x141;ukasiewicz Algebraic Logic models of genetic networks and signaling pathways in cells are formulated in terms of nonlinear dynamic systems with n-state components that allow for the generalization of previous logical models of both genetic activities and neural networks.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Neuroscience; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7114/version/1

Non-viral delivery and optimized optogenetic stimulation of retinal ganglion cells led to behavioral restoration of vision
Provedor de dados: 17
Autores: Ling Gu; Shivaranjani Shivalingaiah; Mathew Ficinski; Edward Wong; Samarendra Mohanty.
Stimulation of retinal neurons using optogenetics via use of chanelrhodopsin-2 (ChR2) has opened up a new direction for restoration of vision for treatment of retinitis pigmentosa (RP). Here, we report non-viral in-vivo electroporation of degenerated retina of adult RP-mice with ChR2-plasmids and subsequent in-vivo imaging of retina to confirm expression. Further, we demonstrate that in addition to efficient non-viral delivery of ChR2 to a specific retinal layer, threshold level of stimulation light needs to be delivered onto the retina for achieving successful behavioral outcome. Measurement of intensity of light reaching the retina of RP-mouse models along with geometrical optics simulation of light propagation in the eye is reported in order to...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2012
URL: http://precedings.nature.com/documents/6869/version/1

NORTH DAKOTA SHOPPER PERCEPTIONS OF GENETICALLY MODIFIED ORGANISMS AND FOOD:  RESULTS OF A WINTER 2003 SURVEY
Provedor de dados: 31
Autores: Wachenheim,   Cheryl J.; Lesch,   William C.
Replaced with revised version of paper 07/23/04.
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Genetic Modification; Consumer Preferences; Food Consumption/Nutrition/Food Safety; Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/23601

Novel approaches, including systems biology, to HIV vaccine research and development: Report from a Global HIV Vaccine Enterprise Working Group
Provedor de dados: 17
Autores: Bali  Pulendran; Rino Rappuoli; Alan Aderem; the Working Group convened by the Global HIV Vaccine Enterprise.
The Global HIV Vaccine Enterprise convened a two-day workshop on August 10-11 2009, at the Fred Hutchison Cancer Research Center offices in Seattle, WA, to discuss the application of novel approaches,including systems biology, to HIV vaccine research and development. The goals of this Working Group were to identify key scientific issues and opportunities that have emerged since the Enterprise Scientific Strategic Plan1 was published in 2005, and to make recommendations to Enterprise stakeholders.
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4798/version/2

Novel approaches, including systems biology, to HIV vaccine research and development: Report from a Global HIV Vaccine Enterprise Working Group
Provedor de dados: 17
Autores: Bali  Pulendran; Rino Rappuoli; Alan Aderem; the Working Group convened by the Global HIV Vaccine Enterprise.
The Global HIV Vaccine Enterprise convened a two-day workshop on August 10-11 2009, at the Fred Hutchison Cancer Research Center offices in Seattle, WA, to discuss the application of novel approaches,including systems biology, to HIV vaccine research and development. The goals of this Working Group were to identify key scientific issues and opportunities that have emerged since the Enterprise Scientific Strategic Plan1 was published in 2005, and to make recommendations to Enterprise stakeholders.
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology; Microbiology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4798/version/1

Novel Cell type-specific aptamer-siRNA delivery system for HIV-1 therapy
Provedor de dados: 17
Autores: Jiehua Zhou; Haitang Li; Shirley Li; John Zaia; John Rossi.
The successful use of small interfering RNAs (siRNAs) for therapeutic purposes requires safe and efficient delivery to specific cells and tissues. Here we demonstrate cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer. The envelope glycoprotein is  expressed on the surface of HIV-1 infected cells, allowing binding and interalization of the aptamer-siRNA chimeric molecules. We demonstrate that the anti-gp120 aptamer-siRNA chimera is specifically taken up by cells expressing HIV-1 gp120, and the appended siRNA is processed by Dicer, releasing an anti-tat/rev siRNA which in turn inhibits HIV replication. We show for the first time a dual functioning aptamer-siRNA chimera in which both the aptamer and the siRNA portions have...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/1299/version/1

Novel network pharmacology methods for drug mechanism of action identification, pre-clinical drug screening and drug repositioning
Provedor de dados: 17
Autores: Jianghui Xiong.
The high rates of failure in oncology drug clinical trials highlight the problems of using pre-clinical data to predict the clinical effects of drugs. Here we present two methodology innovations on network pharmacology modeling. (1) We hypothesize that the gene network associated with cancer outcome in heterogeneous patient populations could serve as a reference for identifying drug effects. We proposed a novel in vivo genetic interaction between genes as &amp;#x2018;synergistic outcome determination&amp;#x2019;, in a similar way to &amp;#x2018;synthetic lethality&amp;#x2019;. We scanned above genetic interactions based on microarray profiling for cancer prognosis, and identified a cluster of important yet epigenetically regulated gene modules. By...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6455/version/1

Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for  Composition Improvements
Provedor de dados: 17
Autores: I. C. Baianu; Valentin  Prisecaru.
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions.  Conventional &amp;#x201c;wet chemistry&amp;#x201d; analytical methods are time-consuming and costly.  As a result, soybean breeders and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive method for composition analysis for academia and industry.  Recent advancements in instrumentation design, such as the application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly improved overall instrument...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6231/version/1

Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for  Composition Improvements.  (v.3)
Provedor de dados: 17
Autores: Valentin  Prisecaru; I. C. Baianu.
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions.  Conventional &amp;#x201c;wet chemistry&amp;#x201d; analytical methods are time-consuming and costly.  As a result, soybean breeders and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive method for composition analysis for academia and industry.  Recent advancements in instrumentation design, such as the application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly improved overall instrument...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Bioinformatics; Earth &amp; Environment; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6231/version/3

Novel NIR Spectroscopy Correlation Approach to Amino Acid Analysis of Soybean Proteins for  Composition Improvements.  (Version 2.0)
Provedor de dados: 17
Autores: Valentin  Prisecaru; I. C. Baianu.
Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions.  Conventional &amp;#x201c;wet chemistry&amp;#x201d; analytical methods are time-consuming and costly.  As a result, soybean breeders and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive method for composition analysis for academia and industry.  Recent advancements in instrumentation design, such as the application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly improved overall instrument...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Bioinformatics; Earth &amp; Environment; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6231/version/2

Novel Techniques for Microspectroscopy and Chemical Imaging Analysis of Soybean Seeds and Embryos
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng  You; Doina M. Costescu.
Novel methodologies are currently being evaluated for the Chemical Analysis of soybean seeds as well as developing mature soybean embryos by FT-IR/NIR Microspectroscopy. This is the first report of FT-NIR Chemical Images obtained with 1 micron resolution. NIR spectra of Proteins, Oil and DNA fibers are obtained for regions as small as 1&amp;#x3bc;^2^.
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6591/version/2

Novel Techniques for Microspectroscopy and Chemical Imaging Analysis of Soybean Seeds and Embryos
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng  You; Doina M. Costescu.
Novel methodologies are currently being&amp;#xd;&amp;#xa;evaluated for the Chemical Analysis of&amp;#xd;&amp;#xa;soybean seeds as well as developing &amp;#xd;&amp;#xa;mature soybean embryos by FT-IR/NIR Microspectroscopy. This is the first report of FT-NIR Chemical Images obtained with 1 micron resolution. NIR spectra of Proteins, Oil and DNA fibers are obtained for regions as small as 1&amp;#x3bc;^2^.
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6591/version/1

Nuclear DNA and protein content evaluation in Taxus plant cell cultures using multiparameter flow cytometry
Provedor de dados: 17
Autores: Vishal Gaurav; Amit Shavit; Susan C. Roberts.
Plant cell cultures of Taxus provide the most reliable production methods for the anti-cancer drug paclitaxel. In order to comprehend the inherent culture heterogeneity and production variability in cell cultures, it is essential that the cellular metabolism is studied at the genomic level. Genomic stability in plant cell cultures is crucial as it affects cell growth and division, metabolite accumulation and protein synthesis. A rapid and efficient method to prepare nuclei suspensions from aggregated cell cultures of Taxus was employed. Methods were subsequently developed to simultaneously stain them for DNA and protein content using Propidium Iodide and Fluorescein Isothiocyanate respectively. Flow cytometry was used to analyze and quantify the DNA...
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics &amp; Genomics; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3809/version/1

Nuclear Medicine and Early Discovery of Disease
Provedor de dados: 17
Autores: I. C. Baianu.
There is an urgent need for the early detection of diseases such as Alzheimer&amp;#x2019;s (AD) and Cancers in order to enable their successful treatment. Cancer is the second major cause of death after Heart Disease, and AD is the third major cause of death with major consequences for the society.&amp;#xd;&amp;#xa;Three major Nuclear Medicine techniques that are established for diagnostic and research purposes are: Positron Emission Tomography (PET) CAT/CT and Nuclear Magnetic Resonance Imaging (NMRI/MRI). These nuclear medicine techniques are discussed together with their clinical and research applications to early diagnosis of Alzheimer&amp;#x27;s Disease (AD) and cancers. Novel approaches to early detection and modeling of AD and cancers are then...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics &amp; Genomics; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6196/version/1

Nucleic acid - protein fingerprints. Novel protein classification based on nucleic acid - protein recognition
Provedor de dados: 17
Autores: Alexander Krylov; Renad Zhdanov.
Protein chemistry uses protein description and classification based on molecular mass and isoelectric point as general features. Enzymes are also compared by enzymatic reaction constants, namely Km and kcat values. Proteins are also studied by binding to different oligonucleotides. Here we suggest a simple experimental method for such a comparison of DNA binding proteins, which we call &amp;#x22;nucleic acid-protein fingerprints&amp;#x22;. The experimental design of the method is based on an use of short oligonucleotides immobilized inside microarray of hydrogel cells - biochip. As a first stage, we solved a simple experimental task: what is the shortest single strand oligonucleotide to be recognized by protein? We tested binding of oligonucleotides from 2...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6955/version/1

Nurturing the seeds of success in the semi-arid tropics
Provedor de dados: 30
Palavras-chave: Bioinformatics; Chickpeas; Pigeonpeas; Sorghum; Mycotoxins; Millets; Hybrids; Groundnuts; Biotechnology; Transgenics.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3270

Objective dysphonia quantification in vocal fold paralysis: comparing nonlinear with classical measures
Provedor de dados: 17
Autores: Max A. Little; Declan A. E. Costello; Meredydd L. Harries.
Clinical acoustic voice recording analysis is usually performed using classical perturbation measures including jitter, shimmer and noise-to-harmonic ratios. However, restrictive mathematical limitations of these measures prevent analysis for severely dysphonic voices. Previous studies of alternative nonlinear random measures addressed wide varieties of vocal pathologies. Here, we analyze a single vocal pathology cohort, testing the performance of these alternative measures alongside classical measures.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;We present voice analysis pre- and post-operatively in unilateral vocal fold paralysis (UVFP) patients and healthy controls, patients undergoing standard medialisation thyroplasty surgery, using jitter, shimmer and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3106/version/1

OFF-FARM WORK AND THE ADOPTION OF HERBICIDE-TOLERANT SOYBEANS
Provedor de dados: 31
Autores: Fernandez-Cornejo,   Jorge; Hendricks,   Chad.
Adoption of herbicide-tolerant (HT) soybeans is proceeding rapidly despite little difference between the net returns to HT versus conventional soybeans. Using a multivariate probit model, we analyze the interaction of off-farm work and adoption and test if farmers adopt HT soybeans because its simplicity and flexibility frees management time.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Herbicide-tolerant soybeans; Technology adoption; Off-farm work; Household models; Crop Production/Industries.
Ano: 2003
URL: http://purl.umn.edu/35133

Oilseed Rape straw for Cultivation of Oyster Mushroom
Provedor de dados: 17
Autores: Gholamali Peyvast; Ahmad Nowroozi; Jamal-Ali Olfati.
Oyster mushroom [_Pleurotus ostreatus_ var. _sajor caju_ (Fr.) Singer] cultivation can play an important role in managing organic waste. It can be cultivated on a wide rang of substrates containing lignin and cellulose. Oyster mushroom was grown on five substrates: Rise straw (Oryza sativa L. var. Alikazemi), Rise straw + Oilseed Rape straw (Brassica napus var. Hyola 401) (75:25 dw /dw), Rise straw + Oilseed Rape straw (50:50 dw /dw), Rise straw + Oilseed Rape straw (25:75 dw /dw) and Oilseed Rape straw alone. Oilseed Rape straw alone and Rise straw + Oilseed Rape straw (25:75 dw /dw) were best for fruit body production of P. ostreatus. Time to fruiting for P. ostreatus was also shorter on Oilseed Rape straw. Protein content of the fruit bodies obtained...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2069/version/1

On Asymmetry in Biology and Nature
Provedor de dados: 17
Autores: I. C. Baianu.
Symmetry has attracted a substantial amount of effort because considerable simplifications are possible in the mathematical and physical treatment of phenomena and natural systems that possess a certain degree of symmetry. Among physical and chemical systems the most widely known are those related to crystals and fluids. Whereas crystals have a lattice structure and a symmetry caused by &amp;#x2018;perfect&amp;#x2019; order which can be classified by mathematical symmetry groups, most fluids have an average isotropic, highly-disordered &amp;#x2018;structure&amp;#x2019; that is often considered to be random.Asymmetry is widely encountered in Biology and ecological systems- from amino acids to trees forests, and tribes, from physiological processes to...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Ecology; Genetics &amp; Genomics; Microbiology; Molecular Cell Biology; Bioinformatics; Earth &amp; Environment; Plant Biology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/7134/version/1

On optical detection of densely labeled synapses in neuropil and mapping  connectivity with combinatorially multiplexed fluorescent synaptic markers
Provedor de dados: 17
Autores: Yuriy Mishchenko.
We propose a new method for mapping neural connectivity optically, by utilizing  Cre/Lox system Brainbow to tag synapses of different  neurons with random  mixtures of different  fluorophores, such as GFP, YFP, etc., and then detecting patterns of fluorophores at different synapses using light microscopy (LM). Such patterns will immediately report the pre- and post-synaptic cells at each synaptic connection, without tracing neural projections from individual synapses to corresponding cell bodies. We simulate fluorescence from a population of densely labeled synapses in a block of hippocampal neuropil, completely reconstructed from electron microscopy data, and show that high-end LM is able to detect such patterns with over 95% accuracy. We conclude,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2010
URL: http://precedings.nature.com/documents/4144/version/1

On Pricing and Vertical Organization of Differentiated Products: The Case of Soybean Seed Industry
Provedor de dados: 31
Autores: Shi,   Guanming; Chavas,   Jean-Paul.
This paper investigates the pricing and vertical organization of differentiated products under imperfect competition. In a multiproduct context, a Cournot model is used to examine how substitution/complementarity relationships among products and vertical structures can affect the exercise of market power. This motivates a generalization of the Herfindahl-Hirschman index (termed VHHI) capturing how market concentration and vertical structures interact to influence prices of differentiated products. The analysis is applied to pricing of soybean seeds in the US over the period 2000-2007. The analysis considers two vertical structures employed by biotech firms: vertical integration and licensing. The econometric analysis finds evidence that vertical...
Tipo: Conference Paper or Presentation
Palavras-chave: Vertical structures; Pricing; Imperfect competition; Seed; Biotechnology; Demand and Price Analysis; Industrial Organization; L13; L4; L65.
Ano: 2009
URL: http://purl.umn.edu/49186

On Real-Time Synthetic Primate Vision
Provedor de dados: 17
Autores: Andrew A. Dankers; Nick Barnes; Walter F. Bischof; Alex Zelinsky.
The primate vision system exhibits numerous capabilities. Some important basic visual competencies include: 1) a consistent representation of visual space across eye movements; 2) egocentric spatial perception; 3) coordinated stereo fixation upon and pursuit of dynamic objects; and 4) attentional gaze deployment. We present a synthetic vision system that incorporates these competencies.We hypothesize that similarities between the underlying synthetic system model and that of the primate vision system elicit accordingly similar gaze behaviors. Psychophysical trials were conducted to record human gaze behavior when free-viewing a reproducible, dynamic, 3D scene. Identical trials were conducted with the synthetic system. A statistical comparison of synthetic...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2007
URL: http://precedings.nature.com/documents/1241/version/1

On the Segregation of Genetically Modified, Conventional, and Organic Products in European Agriculture: A Multi-market Equilibrium Analysis
Provedor de dados: 31
Autores: Moschini,   GianCarlo; Bulut,   Harun; Cembalo,   Luigi.
Evaluating the possible benefits of the introduction of genetically modified (GM) crops must address the issue of consumer resistance as well as the complex regulation that has ensued. In the European Union (EU) this regulation envisions the "co-existence" of GM food with conventional and quality-enhanced products, mandates the labelling and traceability of GM products, and allows only a stringent adventitious presence of GM content in other products. All these elements are brought together within a partial equilibrium model of the EU agricultural food sector. The model comprises conventional, GM and organic food. Demand is modelled in a novel fashion, whereby organic and conventional products are treated as horizontally differentiated but GM products are...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Differentiated demand; Genetically modified crops; Identity preservation; Innovation; Welfare; Production Economics.
Ano: 2005
URL: http://purl.umn.edu/18531

On the social induction of Alzheimer&amp;#x27;s disease: An index theorem aging model for amyloid formation
Provedor de dados: 17
Autores: Rodrick Wallace; Deborah Wallace.
The central &amp;#x27;risk factor&amp;#x27; for Alzheimer&amp;#x27;s disease (AD) is age. From first principles, we construct a mathematical model of protein folding and its in vivo regulation that gives this result in a natural manner. We extend the basic approach using topological information theory methods, and examine a case history of socially-induced premature aging in the United States.
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/6012/version/1

On the status of the Michaelis-Menten equation and its implications for enzymology
Provedor de dados: 17
Autores: Sosale Chandrasekhar.
The Michaelis-Menten equation (MME) is considered to be the fundamental equation describing the rates of enzyme-catalysed reactions, and thus the &amp;#x27;physicochemical key&amp;#x27; to understanding all life processes. It is the basis of the current view of enzymes as generally proteinaceous macromolecules that bind the substrate reversibly at the active site, and convert it to the product in a relatively slow overall sequence of bonding changes (&amp;#x27;turnover&amp;#x27;). The manifested &amp;#x27;saturation kinetics&amp;#x27;, by which the rate of the enzymic reaction (essentially) increases linearly with the substrate concentration ([S]) at low [S] but reaches a plateau at high [S], is apparently modelled by the MME. However, it is argued herein...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Evolutionary Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1637/version/1

Ontology for Biomedical Investigations
Provedor de dados: 17
Autores: Bjoern Peters; The OBI Consortium.
The goal of OBI is to enable a formal representation of biomedical investigations that captures the experimental evidence on which their findings are based. The scope of OBI includes: materials made in and produced for investigations, research objectives, experimental protocols, roles of people in investigations and processing and publication of data gathered in investigations. Use of OBI will allow comparison of experimental data from the wide array of scientific disciplines represented by domain experts in the OBI consortium. OBI follows the principles laid out by the OBO foundry, and integrates tightly with other foundry candidate ontologies, such as GO (www.geneontology.org) and ChEBI (www.ebi.ac.uk/chebi/) whose terms are used to describe biological...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3623/version/1

Open Data in Science
Provedor de dados: 17
Autores: Peter Murray-Rust.
Open Data (OD) is an emerging term in the process of defining how scientific data may be published and re-used without price or permission barriers. Scientists generally see published data as belonging to the scientific community, but many publishers claim copyright over data and will not allow its re-use without permission. This is a major impediment to the progress of scholarship in the digital age. This article reviews the need for Open Data, shows examples of why Open Data are valuable and summarizes some early initiatives in formalizing the right of access to and re-use of scientific data.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1526/version/1

Open flower segregants selected from cajanus platycarpus crosses
Provedor de dados: 30
Autores: Cherian, C.A.
Palavras-chave: Pigeonpeas; Self pollination; Cytoplasm; Cross pollination; Segregation; Crossing over; Biotechnology; Hybridization; Hybrids; Grain.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3187

Open Notebook Science: Perspectives from a newbie
Provedor de dados: 17
Autores: Cameron Neylon.
My group is using an electronic lab notebook based on a Blog format that is being developed in collaboration with the group of Professor Jeremy Frey. Here I discuss how this led to the adoption of an open notebook science approach in my group as well as some of the consequences, both positive and negative, of adopting such an approach.
Tipo: Presentation
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1130/version/1

Open Science: Tools, approaches, and implications
Provedor de dados: 17
Autores: Shirley Wu; Cameron Neylon.
The Pacific Symposium on Biocomputing is an annual meeting whose topics are determined by proposals submitted by members of the community. This document is the proposal for a session on Open Science, submitted for consideration for the PSB meeting in 2009.
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1633/version/1

Open Standards and Resources in Systems Biology: collaborative scale-up toward virtual life
Provedor de dados: 17
Autores: Nicolas Le Novere.
The practise of Systems Biology relies on interfaces. Interfaces&amp;#xd;&amp;#xa;between the entities we study: the paradigm moved from a physical&amp;#xd;&amp;#xa;object centric view toward a relationship-centric one; interfaces&amp;#xd;&amp;#xa;between tools: From the retrieval of the primary data to the fine&amp;#xd;&amp;#xa;analysis of a model&amp;#x27;s behaviour, one uses many tools, more or less&amp;#xd;&amp;#xa;well connected; interfaces between individuals: To build any&amp;#xd;&amp;#xa;non-trivial mechanistic model requires to merge existing work and&amp;#xd;&amp;#xa;gather external expertise.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;If we want these interfaces to be generic enough to allow for anybody&amp;#xd;&amp;#xa;to leverage on existing...
Tipo: Presentation
Palavras-chave: Biotechnology; Data Standards.
Ano: 2006
URL: http://precedings.nature.com/documents/10/version/1

Operacionalizacao da extracao de DNA de especies nativas do cerrado visando analises moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.
bitstream/CPAC-2009/24957/1/p2004_10.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Planta nativa; Indigenous organisms.; Biotecnologia; Biologia Molecular; Cerrado; DNA; Marcador Genético; Marcador Molecular.; Biotechnology; Genetic markers; Molecular biology.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567935

Optimal control circuitry design for the digital p53 dynamics in cancer cell and apoptosis
Provedor de dados: 17
Autores: Rosario M. Ardito Marretta.
Experimental work and theoretical models deduce a &amp;#x22;digital&amp;#x22; response of the p53 transcription factor when genomic integrity is damaged. The mutual influence of p53 and its antagonist, the Mdm2 oncogene, is closed in feedback. This paper proposes an aerospace architecture for translating the p53/Mdm2/DNA damage network into a digital circuitry in which the optimal control theory is applied for obtaining the requested dynamic evolutions of some considered cell species for repairing a DNA damage. The purpose of this paper is not to improve the analysis of the actual mathematical models but to demonstrate the usefulness of such digital circuitry design capable to predict and detect the cell species dynamics for finding more information...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/2870/version/1

Optimal control of bacterial growth for the maximization of metabolite production
Provedor de dados: 5
Autores: Yegorov, Ivan; Mairet, Francis; De Jong, Hidde; Gouze, Jean-luc.
Microorganisms have evolved complex strategies for controlling the distribution of available resources over cellular functions. Biotechnology aims at interfering with these strategies, so as to optimize the production of metabolites and other compounds of interest, by (re)engineering the underlying regulatory networks of the cell. The resulting reallocation of resources can be described by simple so-called self-replicator models and the maximization of the synthesis of a product of interest formulated as a dynamic optimal control problem. Motivated by recent experimental work, we are specifically interested in the maximization of metabolite production in cases where growth can be switched off through an external control signal. We study various optimal...
Tipo: Text
Palavras-chave: Optimal control; Nonlinear dynamical systems; Mathematical modelling; Bacterial growth; Biotechnology.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00486/59791/63028.pdf

Optimising Blue Fluorescent Protein (BFP) for use as a mammalian reporter gene in parallel with Green Fluorescent Protein (GFP).
Provedor de dados: 17
Autores: Etienne Joly.
To obtain an optimised form of BFP for use as a reporter gene in mammalian cells, the brightest available GFP form, EGFP, was mutated at 5 different positions, yielding 8 different mutagenised forms of BFP. The intensity of the fluorescent signals attained in mammalian cells with all these various versions of BFP was analysed by flow cytometry of transiently transfected COS 7 cells. The best mutant obtained can be detected readily both by flow cytometry and fluorescence microscopy, even when expressed together with GFP. To explore whether cellular localization could enhance the fluorescence signals any further, plasmid constructs were made to target optimised versions of GFP and BFP to the nucleus, the endoplasmic reticulum (ER) and the cell surface....
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1259/version/1

Optogenetic control of genetically-targeted pyramidal neuron activity in prefrontal cortex
Provedor de dados: 17
Autores: Michael  V. Baratta; Shinya Nakamura; Peter Dobelis; Matthew B. Pomrenze; Samuel D. Dolzani; Donald  C. Cooper.
A salient feature  of  prefrontal  cortex  organization  is  the  vast diversity  of  cell types  that  support  the  temporal   integration   of   events   required   for   sculpting future   responses.   A   major   obstacle   in   understanding   the   routing   of   information   among   prefrontal   neuronal   subtypes   is   the   inability   to   manipulate   the   electrical   activity   of genetically defined cell types over behaviorally relevant timescales and activity patterns.  To address these constraints, we present here a simple approach for selective activation of prefrontal excitatory   neurons   in   both   in   vitro   and   in   vivo   preparations.   Rat   prelimbic pyramidal neurons   were   genetically   targeted   to   express   a...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Neuroscience.
Ano: 2012
URL: http://precedings.nature.com/documents/7102/version/1

Origin and evolution of tomato production Lycopersicon esculentum in México
Provedor de dados: 65
Autores: Saavedra,Tarsicio Medina; Figueroa,Gabriela Arroyo; Cauih,Jorge Gustavo Dzul.
ABSTRACT:  Lycopersicon esculentum known as tomato, although has an Andean origin is a contribution of Mexico to the world is, being the first agricultural product to be exported. This research aimed to review the literature in relation to the origin and evolution of the production of tomato in Mexico within the historical development of the country. In ancient times, the tomato was cultivated in milpas (open field) and chinampas (artificial islands for riparian agriculture) using sustainable methods. Spanish colonizers showed the tomato to the rest of the world and diversified its uses. In independent Mexico, haciendas and railroads integrated the different farming regions. Production decreased during the Mexican revolution, and with land reform, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopersicon esculentum; Chinampas; Green revolution; Organic production; Biotechnology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300401

Overnight weight loss: relationship with sleep structure and heart rate variability
Provedor de dados: 17
Autores: Walter Moraes; Dalva Poyares; Christian Guilleminault; Agostinho Rosa; Marco Tulio Mello; Adriana Rueda; Sergio Tufik.
Background: Weight loss can be caused by a loss of body mass due to metabolism and by water loss as unsensible water loss, sweating, or excretion in feces and urine. Although weight loss during sleep is a well-known phenomenon, it has not yet been studied in relation to sleep structure or autonomic tonus during sleep. Our study is proposed to be a first step in assessing the relationship between overnight weight loss, sleep structure, and HRV (heart rate variability) parameters.Methods: Twenty-five healthy volunteers received a 487 kcal meal and 200 ml water before experiment. Volunteers were weighed before and after polysomnography. Absolute and relative weight indices were calculated. Time and frequency domain analysis of heart rate variability was...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2342/version/1

Panorama tecnológico de biorreatores para pesquisa de transformação de resíduos sólidos em fertilizantes orgânicos.
Provedor de dados: 14
Autores: INACIO, C. de T.; GOUVEA, S. P.; LAFORET, M. R. C.
Com a finalidade de se realizar um levantamento da atividade de patenteamento de invenções nas categorias de biorreatores - que operam por meio da atividade microbiológica, aeróbica e em escala de teste de bancada ou piloto -, foi estabelecido o estudo de panorama de tecnologias publicadas em documentos de patente. Dentre os principais indicadores desse estudo, foram empregadas as variáveis da evolução temporal dos depósitos de patentes, das áreas de conhecimento das tecnologias, bem como dos países de origem desses depósitos.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Biorreatores; Biotecnologia; Patente; Biotechnology; Patents.
Ano: 2018
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1096703

Parables: applied economics literature about the impact of genetically engineered crop varieties in developing economies
Provedor de dados: 31
Autores: Smale,   Melinda; Zambrano,   Patricia; Falck-Zepeda,   Jose Benjamin; Gruere,   Guillaume P.
A vast literature has accumulated since crop varieties with transgenic resistance to insects and herbicide tolerance were released to farmers in 1996 and 1997. A comparatively minor segment of this literature consists of studies conducted by agricultural economists to measure the farm-level impact of transgenic crop varieties, the size and distribution of the economic benefits from adopting them, consumer attitudes toward GE products, and implications for international trade. This paper focuses only on the applied economics literature about the impact of transgenic crop varieties in non-industrialized agricultural systems, with an emphasis on methods. A number of studies have surveyed the findings for both industrialized and non-industrialized agriculture,...
Tipo: Report
Palavras-chave: Genetically engineered crops; Economic impacts; Technology adoption; Developing economies; Economics methods; Best practices; Biotechnology; Crop Production/Industries.
Ano: 2006
URL: http://purl.umn.edu/55412

Paraoxonase 1 Polymorphism p.Q192R in Patients With Dementia
Provedor de dados: 17
Autores: Tomasz Krzywkowski.
PON1 PCR-RFLP polymorphism frequency and enzyme activity were determined in 223 patients with dementia (94 with AD, 55 with VaD and 74 with MD) and in 100 age and sex matched controls without dementia. I found no statistical significance of genotype frequencies between analyzed groups. &amp;#xd;&amp;#xa;Paraoxonase 1 activity was lower in patients carrying the R allele in locus 192 as compared with the R allele non-carriers. The results showed statistically significant association between PON1 polymorphism and enzyme activity and thus can suggest an important relationship between different isoforms of PON1, enzyme activity and dementia.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Neuroscience.
Ano: 2011
URL: http://precedings.nature.com/documents/6333/version/1

Partial characterization of an exopolysaccharide secreted by a marine bacterium, Vibrio neocaledonicus sp nov., from New Caledonia
Provedor de dados: 5
Autores: Chalkiadakis, Eletherios; Dufourcq, R.; Schmitt, Sophie; Brandily, Christophe; Kervarec, N.; Coatanea, Denis; Amir, H.; Loubersac, Lionel; Chanteau, S.; Guezennec, Jean; Dupont-rouzeyrol, M.; Simon-colin, Christelle.
Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identify NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic Resonance and HPLC-size...
Tipo: Text
Palavras-chave: Biopolymers; Biotechnology; Exopolysaccharide; Fermentation biotechnology.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00144/25501/24036.pdf

Patent application in biotechnology at subclass C12N in Brazil at the period of 2001 to 2005
Provedor de dados: 52
Autores: Buttow,Mônica Elisa; Steindel,Mário.
An analysis of Instituto Nacional de Propriedade Industrial (INPI) patent database from 2001 to 2005 showed that less than 5% of the total patent deposited in the subclass C12N, which referred to "Microorganisms or Enzymes, Compositions thereof, Propagating, Preserving or Maintaining Microorganisms, Mutation or Genetic Engineering, Culture Media", were from national depositors. In contrast, more than 34% of all these deposits came from the United States of America during the same period. Among the national depositors, public universities and government research institutions contributed with 83% over all the deposits, demonstrating that research and development in the field of Biotechnology in Brazil has been concentrated within Brazilian public...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Patents; Biotechnology; Intellectual Property; Technologic Innovation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300003

Pecuniary, Non-Pecuniary, and Downstream Research Spillovers: The Case of Canola
Provedor de dados: 31
Autores: Gray,   Richard S.; Malla,   Stavroula; Tran,   Kien C.
This paper develops an empirical framework for estimating a number of inter-firm and downstream research spillovers in the canola crop research industry. The spillovers include basic research, human capital/ knowledge (as measured through other-firm expenditures), and genetics (as measured through yields of other-firms). The model used to examine spillover effects on research productivity provides evidence that there are many positive inter-firm non-pecuniary research spillovers, which is consistent with a research clustering effect. The second model, which examines spillovers at the level of firm revenue , shows that, while private firms tend to crowd one another, public firm expenditure on basic and applied research creates a crowding-in effect for...
Tipo: Conference Paper or Presentation
Palavras-chave: Basic research; Applied research; Public research expenditures; Private research expenditures; Biotechnology; Research and Development/Tech Change/Emerging Technologies; O3.
Ano: 2005
URL: http://purl.umn.edu/24776

Pepper yellow leaf curl Indonesia virus, a new bipartite begomovirus species that belongs to distinct clade of Old World geminiviruses
Provedor de dados: 17
Autores: Pradeep  Sharma1,2; J. Sakata2; Y. Shibuya2; Masato  Ikegami2.
Begomoviruses are currently emerging as a major threat to vegetable production in many tropical and subtropical regions worldwide. Pepper yellow leaf curl disease (PepYLCD) has been noticed in many Capsicum annum L. producing regions from East Asia especially from Indonesia and causes devastating damage to pepper crop production since 2000. In this study we have cloned and sequenced complete nucleotide of begomoviruses from pepper exhibiting leaf curling and bright yellowing symptoms. Besides, we also determined the occurrence of disease on tomato evoking leaf curl symptoms and ageratum with yellow vein type of symptoms. On the basis of genome organization and sequence homology, these viruses were designated as Pepper yellow leaf curl Indonesia virus...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4162/version/1

Peptides as potent antimicrobials tethered to a solid surface: Implications for medical devices
Provedor de dados: 17
Autores: Ivan A. Gonzalez; XiXi Wong; Dilhari DeAlmeida; Raymond B. Yurko; Simon C. Watkins; Kazi Islam; Ronald C. Montelaro; Ahmed El-Ghannam; Timothy A. Mietzner.
Medical devices are an integral part of therapeutic management; despite their importance, they carry a significant risk of microbial infection. Bacterial attachment to a medical device is established by a single, multiplying organism, leading to subsequent biofilm formation. To date, no preventative measures have impacted the incidence of device-related infection. We report the bidirectional covalent coupling of an engineered cationic antimicrobial peptide (eCAP), WLBU2, to various biological surfaces is accomplished. These surfaces included (i) a carbohydrate-based gel matrix, (ii) a complex polymeric plastic bead, and (iii) a silica-calcium phosphate nanocomposite associated with bone reconstruction. WLBU2-conjugated surfaces are shown to retain potent...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2008
URL: http://precedings.nature.com/documents/1967/version/1

Performance of beef cattle bulls in feed lots and fed on diets containing enzymatic complex
Provedor de dados: 2
Autores: Oliveira, Leonardo Guimarães de; Ferreira, Reginaldo Nassar; Padua, João Teodoro; Ulhoa, Cirano José; Cysneiros, Cristine dos Santos Settimi; Arnhold, Emmanuel.
Current paper evaluates the performance of confined beef cattle supplemented with amylolytic enzyme complex produced by fungus Aspergillus awamori and a commercial product containing multienzyme complex, yeast and MOS. Treatments comprised control (basal diet composed of 16% Mombasa grass silage, 66% ground corn, 3% vitamin nuclear mineral and 15% cottonseed meal), amylase treatment (control diet with the addition of 48.7 saccharifying units kg-1 diet) and compound treatment (control diet with the addition of enzymatic complex composed of 83.2 saccharifying units, 8.8 fibrolytic units, 0.05 g of mannan oligosaccharides and 0.2 g of inactivated yeast Kg-1 of the dry matter diet). The addition of products did not significantly increase daily weight gain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.04.03.02-8 starch; Biotechnology; Feedlot; Ruminant.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/26446

pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers
Provedor de dados: 17
Autores: Corinne Marie; Magali Richard; Gaelle Vandermeulen; Mickael Quiviger; Veronique Preat; Daniel Scherman.
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic Resistance genes, called pFARs. The strategy is based on the suppression of a chromosomal nonsense mutation by a plasmid-borne function. The amber mutation was introduced into the Escherichia coli thyA gene that encodes a thymidylate synthase required for dTMP synthesis, resulting in thymidine auxotrophy. In parallel, a small plasmid vector that carries an amber suppressor t-RNA gene was entirely synthesised. The introduction of pFAR plasmids into an optimised thyA mutant restored normal growth to the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics.
Ano: 2008
URL: http://precedings.nature.com/documents/2395/version/1

Phagocytic Activity and Hexose Monophosphate Shunt Activity of Cultured Human Kupffer Cells upon Zymosan, Erythrocytes, Amoebic Trophozoits, Latex Beads and Nitroimidazole
Provedor de dados: 17
Autores: Rakesh Sharma; Vijay S. Singh.
Phagocytic function of human Kupffer cells was altered by the addition of nitroimidazole and amoebic trophozoits. The hypothesis was that phagocytic activity was dependent on the nature of additives in culture. The Kupffer cells were fractionated and lysosomes were isolated for enzyme estimations in presence of additives. Phagocytic activity function of human Kupffer cells was altered by the addition of nitroimidazole and amoebic trophozoits in Kupffer cell culture. The human Kupffer cells showed take up of zymosan particles larger than 1 micron in diameter and inert interaction with latex particles. The liver endothelial cells or phagocytes constitute a second line of defense in the liver for the phagoctosis function. The nitroimidazole slowed down the...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4300/version/1

PharmGKB:  Capturing knowledge to catalyze pharmacogenomics research
Provedor de dados: 17
Autores: Russ B. Altman.
This is a powerpoint presentation made initially at the Cold Spring Harbor meeting on Pharmacogenomics, November 2006.  It discusses the PharmGKB (http://www.pharmgkb.org/) and how we are building a pipeline for annotation of knowledge.  In particular, we are focusing on pathway knowledge, &amp;#x22;Very important Pharmacogene&amp;#x22; annotations, and curation of the pharmacogenomics literature.  We are building an ontology-based system to capture and aggregate knowledge, for use by our curators.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Pharmacology.
Ano: 2006
URL: http://precedings.nature.com/documents/8/version/1

Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.]
Provedor de dados: 58
Autores: Fernandes Júnior,Paulo Ivan; Lima,Andréa Aparecida de; Passos,Samuel Ribeiro; Gava,Carlos Alberto Tuão; Oliveira,Paulo Jansen de; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro.
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strain selection; Biological nitrogen fixation; Biotechnology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400045

Photoelectrochemical properties of melanin
Provedor de dados: 17
Autores: Arturo Solis; Maria E. Lara; Luis E. Rendon.
Melanin is to the animal kingdom like chlorophyll to the vegetal kingdom(1). Melanin collects energy from lower-energy radiation sources, kicks electrons into excited states, initiating a process that would end up producing chemical energy, similar to the way in which photosynthesis supplies energy to plants. However, the precise roles of melanin during this process are unknown. Here we show that the increase in the electron-transfer properties of melanin is independent of the energy of the incident photons. We found in controlled in vivo assays that melanin has the remarkable capability of converting lower-energy radiation towards a more useful form of energy. Furthermore, we found that melanin can break up water molecules and giving up energy suggesting...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2007
URL: http://precedings.nature.com/documents/1312/version/1

Phytochemical Approach and Bioanalytical Strategy to Develop Chaperone-Based Medications
Provedor de dados: 17
Autores: Bernd B. K. Kastenholz.
Currently, no pharmaceuticals for the etiological treatment of degenerative protein-misfolding diseases (e.g., ALS, Alzheimer&amp;#x2019;s or prion diseases) are commercially available. Therefore, in this technical note theoretical considerations and practical approaches concerning the development of chaperone-based medications from medicinal plants (e.g., Ginkgo biloba) are reviewed and discussed in detail. Phytochaperones and other agents isolated from medicinal plants are proposed to serve as the general basis of drug development in protein-misfolding diseases.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Pharmacology.
Ano: 2007
URL: http://precedings.nature.com/documents/1431/version/1

Pioneer Discovery of secondary gold deposits-unique bacterioforms andplacers in north Goa from deep coremetabiosphere
Provedor de dados: 17
Autores: Nandkumar M. Kamat.
Bacterioform secondary gold deposits were discovered in tilloid overlaying archaean base rock (metagraywacke) in Sanvordem rock formation, at the depth of 65 metres in north Goa , India and were confirmed optically as microbiogenic Gold. Multiple and unique forms were seen. This is claimed to be first report of bacterioform Gold in deep cores in Goa, India and Asia.
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Ecology; Genetics &amp; Genomics; Microbiology; Earth &amp; Environment; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5497/version/1

Plant Genetic Resources for Agriculture, Plant Breeding, and Biotechnology: Experiences from Cameroon, Kenya, the Philippines, and Venezuela
Provedor de dados: 31
Autores: Falck-Zepeda,   Jose Benjamin; Zambrano,   Patricia; Cohen,   Joel I.; Borges,   Orangel; Guimaraes,   Elcio P.; Hautea,   Desiree; Kengue,   Joseph; Songa,   Josephine.
Local farming communities throughout the world face binding productivity constraints, diverse nutritional needs, environmental concerns, and significant economic and financial pressures. Developing countries address these challenges in different ways, including public and private sector investments in plant breeding and other modern tools for genetic crop improvement. In order to measure the impact of any technology and prioritize investments, we must assess the relevant resources, human capacity, clusters, networks and linkages, as well as the institutions performing technological research and development, and the rate of farmer adoption. However, such measures have not been recently assessed, in part due to the lack of complete standardized information...
Tipo: Working or Discussion Paper
Palavras-chave: Plant breeding; Biotechnology; Public research; Funding; Innovation systems; Capacity building; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2008
URL: http://purl.umn.edu/42329

Plantas transgênicas e a microbiota do solo.
Provedor de dados: 14
Autores: REIS JÚNIOR, F. B. dos; MENDES, I. de C.; HUNGRIA, M.
ABSTRACT: Soil micro-organisms represent a key hole in the nutrient cycle and in the maintenance of the fertility, besides execute another variety of important functions for the plants and the environment. With the possibility of the transgenic crops to cause negative impacts on the soil microbial communities, emerge the necessity to deep evaluate the real extensions of these impacts. In such case, this document presents some discussions about the interactions between transgenic plants and soil micro-organisms, showing results from different studies conducted by many reseachers in the world and focusing in the evaluated parameters and the methodologies utilised to analyse the microbial communities.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Microbial flora; Plant diseases; Pests of plants; Resistance.; Biotecnologia; Doença de Planta; Herbicida; Microbiologia do Solo; População Microbiana; Praga de Planta; Planta Transgênica; Resistência.; Herbicides; Biotechnology; Soil biology; Transgenic plants.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/569840

PMEM of carabid beetles in the agro-ecosystem: Prospects and problems
Provedor de dados: 17
Autores: Kai  U. Priesnitz; Ullrich  Benker.
The comparison of carabid communities sampled at three different field sites during 5 years of ecological biosafety research provides useful information for the design of a post market environmental monitoring of this arthropod family. Each evaluated field site demonstrated a unique composition of carabid species. A baseline for the carabid beetle communities would therefore be necessary for each field site to detect any changes in its composition over time. Aside from the influence of the location, the impacts of weather, the date of sampling and of course the chosen sampling method have to be considered. A standardized method is a basic requirement for a replicable assessment. The number of traps per square meter, and the size and the design of the traps...
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4445/version/1

Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures
Provedor de dados: 52
Autores: Carone,Sílvia Bicudo; Santa-Catarina,Claudete; Silveira,Vanildo; Floh,Eny Iochevet Segal.
The aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants, explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA and kinetin, under light or in the dark, during successive subcultures. PAs levels followed a basipetal decrease in diploid and an increase in haploid, pith tissues. A similar pattern of total PAs (free + conjugated) was observed for the callus of diploid and haploid plants maintained in the light, and for the haploid callus in the dark, whereas the diploid callus in the dark showed a constant increase in total PAs levels until the end of culture. The PA increase in the diploid callus in the dark was related to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Nicotiana tabacum; Ploidy; Putrescine; Spermidine; Spermine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020

Polyethylenimine-Modified Multiwalled Carbon Nanotubes for Plasmid DNA Gene Delivery
Provedor de dados: 17
Autores: Bo-Zhang  Yu; Ji-Fei Ma; Wen-Xin Li.
An efficient molecular delivery technique based on the transporting high-molecular-weight PEI 600K-modified multiwalled carbon nanotubes (PEI 600K-MWCNTs) into cell membranes is reported. The PEI 600K-MWCNTs exhibit low cytotoxicity and its associated plasmid DNA (pDNA) is delivered to cells efficiently, and the green fluorescent protein (GFP) levels up to 18 times higher than that of naked DNA were observed.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/2753/version/1

Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense oligonucleotide
Provedor de dados: 17
Autores: Xiaolong Wang; Deming Gou.
Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process. Here we report a novel thermocyclic reaction, polymerase-endonuclease amplification reaction (PEAR), for the amplification of oligonucleotides. A target oligonucleotide and a tandem repeated antisense probe are subjected to repeated cycles of denaturing, annealing, elongation and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and thermostable endonuclease (PspGI) cleavage releases monomeric duplex oligonucleotides. Each round of PEAR achieves &amp;#x3e;100-fold...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology.
Ano: 2009
URL: http://precedings.nature.com/documents/3711/version/1

Post-market monitoring: legal framework in Brazil and first results
Provedor de dados: 17
Autores: Marcia A. Melo; Ederson  A. Kido; Paulo P. Andrade.
Brazil is presently (2010) the second largest producer of Genetically Modified (GM) crops. The legal framework to support both research activities and the commercial release of Genetically Modified Organisms (GMOs) is, however, very recent, as the Biosafety Law dates back from 2005. After the indispensable risk analysis, done by National Technical Committee for Biosafety (CTNBio), a GM plant can be approved for commercial release. Nevertheless, as stated in the Normative Resolution nr. 5 (NR-5), 2008, the post-market monitoring is mandatory. However, the first GM crop in Brazil, the herbicide tolerant soybean, was harvested in 2005. The CTNBio asked, then, for a monitoring plan and the applicant designed a complex study focused on case-specific monitoring....
Tipo: Poster
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4528/version/1

Potencial biotecnológico de espécies vegetais oleaginosas ocorrentes em comunidades extrativistas do Acre.
Provedor de dados: 14
Autores: MENEGUETTI, N. F. S. P.; SIVIERO, A.
O presente capítulo tem por objetivo analisar o potencial biotecnológico das principais espécies oleaginosas vegetais que ocorrem em áreas extrativistas do estado do Acre, Brasil.
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Amazônia Ocidental; Tucumã; Castanha do brasil; Acre; Western Amazon; Amazonia Occidental.; Produto florestal não madeireiro (PFNM); Productos forestales no madereros; Grasas y aceites vegetales; Oenocarpus mapora; Planta Oleaginosa; Tucum; Bacuri; Platonia Insignis; Copaíba; Pracaxi; Pentaclethra Macroloba; Castanha do Para; Bertholletia Excelsa; Cupuaçu; Cacau; Theobroma Cacao; Andiroba; Carapa Guianensis; Ucuuba; Virola Surinamensis.; Biotecnologia; Gordura Vegetal; Óleo Vegetal; Buriti; Murumuru; Astrocaryum Murumuru; Patauá; Oenocarpus Bataua; Bacaba; Nontimber forest products; Plant fats and oils; Biotechnology; Mauritia flexuosa; Euterpe precatoria; Attalea phalerata; Astrocaryum aculeatum; Copaifera; Theobroma; Brazil nuts.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1119046

Potential and environmental concerns of ethanol production from sugarcane molasses in Pakistan
Provedor de dados: 17
Autores: Tahir Rashid; Zafar Altaf.
Energy security and climate change imperatives require large-scale substitution of petroleum-based fuels as well as improved vehicle efficiency4. Biofuels have become one of the fastest growing markets in the world - at 15% growth a year. In fact, until recently, Pakistan was the second largest exporter of sugarcane ethanol to the European Union - a preferential status we have since lost because of WTO obligations and dumping complaints. Several distilleries have planned to close down in light of this fact. Instead of curbing production of fuel ethanol, however, we should redirect it to the domestic market. Pakistan produces around 2 million tons molasses annually and over 90% is exported earning only $47 million. This quantity of molasses will produce...
Tipo: Manuscript
Palavras-chave: Biotechnology; Earth &amp; Environment.
Ano: 2008
URL: http://precedings.nature.com/documents/1499/version/1

Potential Impacts of Bt Eggplant on Economic Surplus and Farmers’ Health in India
Provedor de dados: 31
Autores: Krishna,   Vijesh V.; Qaim,   Matin.
In this article, the potential impacts of Bt eggplant technology in Indian agriculture are analyzed. Several proprietary Bt hybrids are likely to be commercialized in the near future. Based on field trial data, it is shown that the technology can significantly reduce insecticide applications and increase effective yields. Comprehensive farm survey data are used to project farm level effects and future adoption rates. Simulations show that the aggregate economic surplus gains of Bt hybrids could be around US $108 million per year. Consumers will capture a large share of these gains, but farmers and the innovating company will benefit too. As the company has also shared its technology with the public sector, Bt open-pollinated varieties might become...
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Bt eggplant; Economic surplus; Health costs; Pesticides; Public-private partnership; Health Economics and Policy; Research and Development/Tech Change/Emerging Technologies.
Ano: 2007
URL: http://purl.umn.edu/9909

Predicting Prostate Cancer Aggressiveness through a Nanoparticle Test
Provedor de dados: 17
Autores: Qun Huo; Shannon Sullivan; Hillari Hallquist; Sally Litherland; David Decker; Inoel Rivera-Ramirez.
Prostate cancer (PCa) is the most common malignancy and the second leading cause of cancer death in American men. Due to the lack of accurate tests to distinguish aggressive cancer from indolent tumor, prostate cancer is often over-treated. Post-surgery pathology analysis revealed that 30% of tumors removed by radical prostatectomy are deemed clinically insignificant and would not have required such invasive treatment.^1^ Over-diagnosis and treatment of low-risk prostate cancer has serious and long-lasting side effect: as high as 70% of the patients who receive radical prostatectomy treatment will suffer a loss of sexual potency that cannot be remedied by drugs such as sildenafil citrate.^2^ We herein report a simple nanoparticle-serum protein adsorption...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2011
URL: http://precedings.nature.com/documents/6409/version/1

Prediction of Evolutionarily important catalytic amino acid of Mycobacterium tuberculosis O-Succinylbenzoate synthase through in silico mutational analysis
Provedor de dados: 17
Autores: Babajan B; Chaitanya M; Anuradha CM; Suresh Kumar Chitta.
The emergence of tuberculosis resistant to multiple, first- and second-line antibiotics poses challenges to a global control strategy that relies on standard drug treatment regimens. The high drug-resistant strains of Mycobacterium tuberculosis (Mtb) have been implicated in outbreaks and have been found throughout the world; a comprehensive understanding, the magnitude of this threat requires an accurate assessment of the worldwide burden of resistance. In an attempt to design anti-TB drugs, the target chosen is a key enzyme of Mtb, O-Succinylbenzoate synthase (OSBS), which is an attractive target for its role in electron transport chain as OSBS is not available in humans.  An attempt has been to built the 3-D structure of Mtb-OSBS using online Swiss model...
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology; Pharmacology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3776/version/1

Prediction of specific virus outbreaks made from the increased concentration of a new class of virus genomic peptides, replikins.
Provedor de dados: 17
Autores: Samuel Bogoch; Elenore S. Bogoch.
Advance warning of pathogen outbreaks has not been possible heretofore. A new class of genomic peptides associated with rapid replication was discovered and named replikins. Software was designed to analyze replikins quantitatively. Replikin concentration changes were measured annually prior to, and &amp;#x201c;real time&amp;#x201d; every few days during, the 2009 H1N1 influenza pandemic. Replikins were seen by both linear sequence representation and three-dimensional X-ray diffraction, and found to expand on the virus hemagglutinin surface prior to and during the H1N1 pandemic.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;A highly significant increased concentration of virus replikins was found a) retrospectively in three pandemics from 1918 to 1999 (14,227...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Immunology; Microbiology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6279/version/1

Present and future of proteomics data curation at the PRIDE database
Provedor de dados: 17
Autores: Juan Antonio Vizcaino; Henning Hermjakob; Lennart Martens.
Significant progress has been made in improving the accessibility and utility of the large amounts of generated high-throughput proteomics data by the introduction of publicly available proteomics repositories. One such repository is PRIDE (the &amp;#x2018;PRoteomics IDEntifications&amp;#x2019; database, &amp;#x22;http://www.ebi.ac.uk/pride&amp;#x22;:http://www.ebi.ac.uk/pride). PRIDE stores mass spectrometry related data, including peptide and protein identifications, mass spectra and valuable additional metadata.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;At present, data curation in PRIDE is limited to data submission support. The format in which all submissions need to take place is PRIDE XML. Mass spectrometry derived data is very heterogeneous in terms of...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3222/version/1

Private Research and Public Goods: Implications of Biotechnology for Biodiversity
Provedor de dados: 31
Autores: Raney,   Terri; Pingali,   Prabhu L.
The pattern of crop genetic diversity has changed over the past two centuries with the modernization of agriculture, accelerating with the advent of the green revolution. Since the green revolution, the locus of agricultural research has shifted from the public to the private sector. The growing importance of the private sector in agricultural R&amp;D is changing the types of crop technologies that are developed and the ways they are delivered to farmers. The spread of transgenic crops will influence crop genetic diversity, but their implications for the availability of plant genetic resources and the resilience of agricultural ecosystems are not entirely clear. Transgenic crops may increase or decrease crop genetic diversity, depending on how they are...
Tipo: Working or Discussion Paper
Palavras-chave: Biodiversity; Biotechnology; Agricultural Research; Transgenic Crops; Green Revolution.; O13; O33; Q16; Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/23805

ProbCD: enrichment analysis accounting for categorization uncertainty
Provedor de dados: 17
Autores: Ricardo V&amp;#xea;ncio; Ilya Shmulevich.
As in many other areas of science, systems biology makes extensive use of statistical association and significance estimates in contingency tables, a type of categorical data analysis known in this field as enrichment (also over-representation or enhancement) analysis. In spite of efforts to create probabilistic annotations, especially in the Gene Ontology context, or to deal with uncertainty in high throughput-based datasets, current enrichment methods largely ignore this probabilistic information since they are mainly based on variants of the Fisher Exact Test. We developed an open-source R package to deal with probabilistic categorical data analysis, ProbCD, that does not require a static contingency table. The contingency table for&amp;#xd;&amp;#xa;the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/369/version/1

Produção de material vegetativo de videira livre de virus no submédio São Francisco.
Provedor de dados: 14
Autores: LEAO, P. C. de S.; MELO, N. F. de; PIRES, E. da L.
Tem como objetivo produzir material vegetativo livre de virus das principais cultivares de videira de interesse comercial para o submedio Sao Francisco, proporcionando aos produtores e viveiristas da Regiao Nordeste do Brasil a possibilidade de disporem de mudas certificadas sadias e de elevada qualidade, evitando a disseminacao de viroses e, com isto, permitindo a implantacao de vinhedos homogeneos e de elevada produtividade.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Videira; Submedio Sao Francisco; Grape; Diseases; Resistance.; Uva; Biotecnologia; Doença; Muda; Meristema; Micropropagação; Resistência; Vírus.; Biotechnology; Meristems; Micropropagation; Seedlings.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/133465

Producing recombinant commercial proteins
Provedor de dados: 5
Autores: Cadoret, Jean-paul; Bardor, M; Lerouge, P; Cabigliera, M; Henriquez, Vitalia; Carlier, Aude.
Extraction of natural substances and chemical synthesis are the main sources of pharmaceutical molecules. When possible, one may transfer the gene of the molecule in living cells creating individual factories producing on demand and in a safe way the requested molecule. Today, bacteria, yeast, mammalian cells and plants constitute the main plate-forms for various commercial products. Microalgae present numerous advantages and could offer a powerful tool for the production of commercial molecules in a near future.
Tipo: Text
Palavras-chave: Genetics; Recombinant; Algae; Pharmaceuticals; Gene transfer; Biotechnology; Génétique; Recombinant; Algues; Pharmaceutique; Transfert de gène; Biotechnologie.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4045.pdf

Product Life Cycles and Innovation in the US Seed Corn Industry
Provedor de dados: 31
Autores: Magnier,   Alexandre; Kalaitzandonakes,   Nicholas G.; Miller,   Douglas J.
The purpose of this study is to evaluate potential changes in the length of product life cycles in the US seed corn industry. We use the observed survival time on the market for hybrids sold during 1997-2009 to conduct a survival analysis. Our empirical results show that the expected lifetimes for corn hybrids with single biotech traits are 5-15% longer than for hybrids with multiple (stacked) traits, and the expected lifetimes for conventional corn are 13-17% longer than stacked hybrids. Also, the product life cycles for all types of hybrids have decreased over the past twelve years (especially after 2004), but the rate of decline is roughly similar across hybrid types. Based on this evidence, we conclude that the shorter product life cycles are closely...
Tipo: Journal Article
Palavras-chave: Accelerated failure time; Biotechnology; Product life cycle; Survival analysis; Productivity Analysis; Research and Development/Tech Change/Emerging Technologies; Research Methods/ Statistical Methods; Risk and Uncertainty.
Ano: 2010
URL: http://purl.umn.edu/93557

Production and characterization of tyrosinase activity in Pycnoporus sanguineus CCT-4518 crude extract
Provedor de dados: 58
Autores: Duarte,Lívia Teixeira; Tiba,Joyce Batista; Santiago,Mariângela Fontes; Garcia,Telma Alves; Bara,Maria Teresa Freitas.
Tyrosinase is an enzyme of industrial interest. The production and characterization of tyrosinase from P. sanguineus CCT-4518 were investigated. The selection of inductors, luminosity influence, inoculum size and type of culture medium on the production of tyrosinase and the effect of inhibitors on enzyme activity were performed. Optimum conditions for intracellular tyrosinase production was observed after 2 days using 0.15% L-tyrosine as inducer, in the presence of light, with inoculum size of 10 mycelium discs, using 2% malt extract broth medium, incubated at 30°C, and constant agitation of 150 rpm. Tyrosinase activity was completely inhibited by the addition of 6 mM salicylhydroxamic acid or phenylthiourea, however an inhibition of 4.15% was recorded by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pycnoporus sanguineus; White-rot fungi; Tyrosinase; MBTH; Biotechnology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100003

Production and mechanical evaluation of biodegradable composites by white rot fungi
Provedor de dados: 64
Autores: Teixeira,Juan Lopes; Matos,Maxwell Paca; Nascimento,Brenno Lima; Griza,Sandro; Holanda,Francisco Sandro Rodrigues; Marino,Regina Helena.
ABSTRACT The production of plastic packing grows worldwide, resulting in the accumulation of these materials in the environment due to improper disposal and problems related to degradation. Alternatively, composites produced with agricultural residues and filamentous fungi may exhibit physical and mechanical properties similar to or greater than expanded polystyrene, depending on the fungal species and substrate of the cultivation. In the literature, no reports were found on the use of coconut powder and edible fungi of white rot in the mechanical properties of composites. Thus, the objective of this work was to select fungal isolates and to evaluate the period of cultivation in the mechanical resistance of composites produced in coconut powder...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Edible fungi; Coconut powder.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600676

Production of biocomposites from the reuse of coconut powder colonized by Shiitake mushroom
Provedor de dados: 64
Autores: Matos,Maxwell Paca; Teixeira,Juan Lopes; Nascimento,Brenno Lima; Griza,Sandro; Holanda,Francisco Sandro Rodrigues; Marino,Regina Helena.
ABSTRACT The demand for biodegradable composite has grown worldwide in recent years, mainly in order to reduce environmental contamination by structural materials produced from the oil industry. The objective of this study was to evaluate the growth of isolates from the edible mushroom “Shiitake” (Lentinula edodes) in substrate coconut powder-based supplemented with wheat bran, as well as to analyze the influence of fungi growing period and drying time of the colonized substrate on the mechanical properties of the composite, in order to produce a biodegradable composite. The mycelial density is not influenced by the type of hyphae of L. edodes. Drying of the composite does not influence the residual odor, depending on the isolate. The compressive strength...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Fungiculture; Biodegradable composite.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100212

Production of flavor compounds from olive mill waste by Rhizopus oryzae and Candida tropicalis
Provedor de dados: 58
Autores: Guneser,Onur; Demirkol,Asli; Yuceer,Yonca Karagul; Togay,Sine Ozmen; Hosoglu,Muge Isleten; Elibol,Murat.
Abstract The purpose of this study was to investigate the production of flavor compounds from olive mill waste by microbial fermentation of Rhizopus oryzae and Candida tropicalis. Olive mill waste fermentations were performed in shake and bioreactor cultures. Production of flavor compounds from olive mill waste was followed by Gas Chromatography–Mass spectrometry, Gas chromatography- olfactometry and Spectrum Sensory Analysis ®. As a result, 1.73-log and 3.23-log cfu/mL increases were observed in the microbial populations of R. oryzae and C. tropicalis during shake cultures, respectively. C. tropicalis can produce a higher concentration of d-limonene from olive mill waste than R. oryzae in shake cultures. The concentration of d-limonene was determined as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olive mill waste; Biotechnology; Microbial fermentation; Bioflavor; Agro-waste.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200275

Production of Transgenic Cloned Miniature Pigs with Membrane-bound Human Fas Ligand (FasL) by Somatic Cell Nuclear Transfer
Provedor de dados: 17
Autores: Jae Goo Seol; Soo Hyun Kim; Dong-Il Jin; Seung Pyo Hong; Ji Yeon Yoo; Ki Myung Choi; Young Chul Park; Yun Jin Yun; Kwang Wook Park; Jae Young Heo.
Cell-mediated xenograft rejection, including NK cells and CD8+ CTL, is a major obstacle in successful pig-to-human xenotransplantation. Human CD8+ CTL and NK cells display high cytotoxicity for pig cells, mediated at least in part by the Fas/FasL pathway. To prevent cell-mediated xenocytotoxicity, a membrane-bound form of human FasL (mFasL) was generated as an inhibitor for CTL and NK cell cytotoxicity that could not be cleaved by metalloproteinase to produce putative soluble FasL. We produced two healthy transgenic pigs harboring the mFasL gene via somatic cell nuclear transfer (SCNT). In a cytotoxicity assay using transgenic clonal cell lines and transgenic pig ear cells, the rate of CD8+ CTL-mediated cytotoxicity was significantly reduced in transgenic...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Immunology.
Ano: 2010
URL: http://precedings.nature.com/documents/4539/version/1

PRODUCTION SYSTEMS COMPETITION AND THE PRICING OF INNOVATIONS: AN APPLICATION TO BIOTECHNOLOGY AND SEED CORN
Provedor de dados: 31
Autores: Alexander,   Corinne E.; Goodhue,   Rachael E.
Farmers now choose production systems rather than individual inputs.  This change may limit biotechnology innovation rents.  We model a corn farmer's production choice among four systems: traditional, herbicide-resistant, insect resistant, and high-oil.  We compare actual seed and chemical prices to the maximum rent the biotechnology firm can obtain.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Corn; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/21646

PROM Khad an efficient source of P to replace the costly chemical Phosphatic Fertilizer
Provedor de dados: 17
Autores: DMR Sekhar; MK Katewa; MS Shaktawat.
The subsidy on phosphate fertilizers rose to an unacceptable level while alternate cost effective technologies are available. Fertilizer industry needs to develop innovative products using the recent advances in the agricultural sciences. Manure producing industries to be supplied rock phosphate mineral (at subsidized costs) as being supplied to chemical fertilizers industries suitable to produce PROM Khad (Phosphate Rich Organic Manure).
Tipo: Manuscript
Palavras-chave: Biotechnology; Earth &amp; Environment.
Ano: 2012
URL: http://precedings.nature.com/documents/6848/version/1

Proof of principle for shotgun DNA mapping by unzipping
Provedor de dados: 17
Autores: Lawrence J. Herskowitz; Anthony L. Salvagno; R. Andy Maloney; Linh N. Le; Steven J. Koch.
Ability to map polymerases and nucleosomes on chromatin is important for understanding the impact of chromatin remodeling on key cellular processes.  Current methods (such as ChIP and ChIP-chip) have produced a wealth of information that demonstrates this importance, but key information is elusive in these ensemble methods.  We&amp;#x2019;re pursuing a new single-molecule chromatin mapping method based on unzipping native chromatin molecules with optical tweezers.  The first step we are taking towards this ability is shotgun DNA mapping (SDM).  This is the ability to identify the genomic location of a random DNA fragment based on its naked DNA unzipping forces compared with simulated unzipping forces of a published genome.  We show that ~32 separate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics.
Ano: 2010
URL: http://precedings.nature.com/documents/2808/version/2

Proof of principle for shotgun DNA mapping by unzipping
Provedor de dados: 17
Autores: Lawrence J. Herskowitz; Anthony L. Salvagno; R. Andy Maloney; Linh N. Le; Steven J. Koch.
Ability to map polymerases and nucleosomes on chromatin is important for understanding the impact of chromatin remodeling on key cellular processes.  Current methods (such as ChIP and ChIP-chip) have produced a wealth of information that demonstrates this importance, but key information is elusive in these ensemble methods.  We&amp;#x2019;re pursuing a new single-molecule chromatin mapping method based on unzipping native chromatin molecules with optical tweezers.  The first step we are taking towards this ability is shotgun DNA mapping (SDM).  This is the ability to identify the genomic location of a random DNA fragment based on its naked DNA unzipping forces compared with simulated unzipping forces of a published genome.  We show that ~32 separate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics.
Ano: 2009
URL: http://precedings.nature.com/documents/2808/version/1

Protein and DNA evolution: Statistics and combinatorics are the rules of the game
Provedor de dados: 17
Autores: Arunava Goswami; Srimoyee Duttagupta; Chirantan Chakraborty; Amita Pal; Pabitra Pal Choudhury; Sk Sarif Hassan.
Let us assume AVMAYDRYVAIC or MAYDRYVAICCP, most important signature domain of&amp;#xd;&amp;#xa;olfactory receptor (OR) gene family assembled in evolution following simplest possible laws of statistics which says the addition of V after A in AVMAYDRYVAIC or addition of A after M are independent events. There are 20 amino acids present in nature so the probability of joint occurrence of such events is 1/400. Therefore for making a contiguous string of&amp;#xd;&amp;#xa;AVMAYDRYVAIC or MAYDRYVAICCP, the probability would be 1 out of 2012. Over millions of years of evolution this has certainly happened and that is why we see such a signature sequence in OR1D2, OR1D subfamily member 2 as per &amp;#x22;HORDE...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4859/version/1

Public Policy and Endogenous Beliefs: The Case of Genetically Modified Food
Provedor de dados: 31
Autores: Lusk,   Jayson L.; Rozan,   Anne.
When individuals have limited information and are uncertain about the quality of a good, government policy, or the lack thereof, can serve as a signal to consumers about the likelihood of realizing alternatives states of nature. In this paper, we focus on a controversial beliefs about government intervention: the market for genetically modified food. Data from a mail survey were used to estimate an econometric model where beliefs about labeling policy, beliefs about the safety of genetically modified food, and willingness to consume genetically modified food are endogenously determined. Results indicate that consumers who believe the government has a mandatory labeling policy for genetically modified food are more likely to believe genetically modified...
Tipo: Journal Article
Palavras-chave: Biotechnology; Food labeling policy; Genetically modified food; Trivariate probit; Demand and Price Analysis; Food Consumption/Nutrition/Food Safety.
Ano: 2008
URL: http://purl.umn.edu/42460

PUBLIC SECTOR PLANT BREEDING IN A PRIVATIZING WORLD
Provedor de dados: 31
Autores: Thirtle,   Colin G.; Srinivasan,   Chittur S.; Heisey,   Paul W.
Intellectual property protection, globalization, and pressure on public budgets in many industrialized countries have shifted the balance of plant breeding activity from the public to the private sector. Several economic factors influence the relative shares of public versus private sector plant breeding activity, with varying results over time, over country, and over crop. The private sector, for example, dominates corn breeding throughout the industrialized world, but public and private activities in wheat breeding differ widely in Western Europe, different regions of the United States, Canada, and Australia. Public sector involvement in plant breeding may have benefits to society that the private sector's activities may not, fostering greater sharing of...
Tipo: Report
Palavras-chave: Plant breeding; Economics; Public sector; Private sector; Research policy; Biotechnology; Intellectual property; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/33775

PUBLIC-PRIVATE AGREEMENTS FOR EMERGING TECHNOLOGIES IN AGRICULTURE
Provedor de dados: 31
Autores: Johnson,   Marc A.
Land Grant Universities are partnering with private firms to fulfill the university mission. Public and private entities can come together in mutually beneficial activities, measured by fulfillment of each party's mission. Rules of engagement are discussed. Six actual agreements currently in operation between Kansas State University and private entities represent a range of types of public-private agreements.  The principal components of each agreement are described and the benefits to each party enumerated in the context of organizational missions.
Tipo: Journal Article
Palavras-chave: Biotechnology; Commercialization; Intellectual property; Joint venture; Land Grant Universities; Nonprofit corporation; Patent licensing; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/14687

Purification and physicochemical studies of metalloprotease, cotinifolin from Euphorbia cotinifolia
Provedor de dados: 17
Autores: Reetesh Kumar.
A detailed procedure for the purification of cotinifolin from the latex of Euphorbia cotinifolia using ion exchange chromatography and hydrophobic interaction chromatography is described.&amp;#xd;&amp;#xa;Various biochemical characteristics of cotinifolin and other enzymatic properties along with spectroscopic parameters like circular dichorism and fluorescence have been reported. Cotinifolin is stable over a wide range of pH and under extreme conditions of chaotrops and organic solvents where most proteins lost their functions.  Further studies on the identification of physiological substrates, biophysical and structural studies will give insight into the utility of the enzyme in the biotechnological industries and protein engineering.
Tipo: Presentation
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6397/version/1

Pyrosequencing/Sanger Plant Genome Assembly (Limitations, Problems And Solutions) - On The Way To Cucumber (Cucumis sativus L. cv. Borszczagowski) Draft Genome Sequence Publishing
Provedor de dados: 17
Autores: Rafal Woycicki; Zbigniew Przybecki.
Genome sequencing give us an opportunity to broaden the range and improve the quality of omics studies. New sequencing technologies make it possible to achieve the sequencing reads fast and cheap. Since the assembly step of such next generation reads is still not well standardized it is the most cumbersome part of sequencing projects.&amp;#xd;&amp;#xa;We focus here on the wide range of approaches undertaken to assemble the draft cucumber (Cucumis sativus L. cv. Borszczagowski) genome done by using 12x coverage of pyrosequencing Titanium chemistry shotgun paired and unpaired reads combined with Sanger reads.
Tipo: Poster
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4630/version/1

QSAR and Docking Studies of Gallic Acid Derivatives for Anticancer and Immunomodulatory Activity
Provedor de dados: 17
Autores: Dharmendra Kumar Yadav; Feroz Khan; Arvind Singh  Negi.
Immuno-modulation is the process of alteration in immune response due to foreign intrusion of molecules inside the body. In the present communication QSAR and molecular docking studies for immuno-modulatory activity of derivatives of gallic acid were performed. Molecular docking studies showed higher binding affinity with INF&amp;#x3b1;-2, IL-6, and IL-4 receptors. Binding site residues indicate hydrogen bonds formation with gallic acid derivative compounds G-3, G-4, G-5, G-6, G-7 and G-10. Further screening through quantitative structure activity relationship model establishes the immuno-modulatory activity of the compounds G-4, G-7, G-9, G-10, G-12 and G-13. Activity was predicted through a robust statistical model, developed by forward feed multiple...
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Chemistry; Pharmacology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6620/version/1

Quality decay and shelf-life study of fresh celery (Apium graveolens L.) grown under different nitrogen fertilization
Provedor de dados: 17
Autores: Valeria Rizzo; Giuseppe  Muratore; Marco Antonio Russo; Riccardo Iacona; Adalgisa Belligno.
Nitrogen fertilization is useful and widespread to obtain higher growth and quality of productions. To evaluate the influence of nitrogen-rich fertilizers on the quality characteristics, shelf-life tests of celery grown with two levels (80-120 kg ha-1) of two different nitrogen fertilizers were performed. Celery plants were selected and packed in two plastic films (anti-fog polyolefin and micro-perforated polypropylene). Nitrate contents, weight loss, hardness, changes in colour parameters and total phenols, were studied in both packaging solutions during storage. Results showed that celery fertilized with organic nitrogen and packed in anti-fog polyolefin reached a shelf-life of 37 days. Not treated sample packed in micro-perforated polypropylene was not...
Tipo: Manuscript
Palavras-chave: Biotechnology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4180/version/1

Quantification of Cell Movement Reveals Distinct Edge Motility Types During Cell Spreading
Provedor de dados: 17
Autores: Benjamin J. Dubin-Thaler; Jake M. Hofman; Harry V. Xenias; Ingrid A. Spielman; Anna H. Shneidman; Lawrence David; Hans-Guenther Dobereiner; Chris Wiggins; Michael  P. Sheetz.
Actin-based motility is central to cellular processes such as migration, bacterial engulfment, and cancer metastasis, and requires precise spatial and temporal regulation of the cytoskeleton. We studied one such process, fibroblast spreading, which involves three temporal phases: early, middle, and late spreading, distinguished by differences in cell area growth. In these studies, aided by improved algorithms for analyzing edge movement, we observed that each phase was dominated by a single, kinematically and biochemically distinct cytoskeletal organization, or motility type. Specifically, early spreading was dominated by periodic blebbing; continuous protrusion occurred predominantly during middle spreading; and periodic contractions were prevalent in...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/391/version/1

Quantifying the potential of microalgae to remove nutrients from wastewater
Provedor de dados: 5
Autores: Martinez, Carlos; Mairet, Francis; Plaza, Luis; Sciandra, Antoine; Bernard, Olivier.
The main resources limiting microalgae growth are typically phosphorus, nitrogen, and light. Based on the theory of the light limited chemostat, the variable cell quota approach, and photoacclimation models, we build a mathematical model for describing microalgae growth under limitation by these resources. The model is calibrated with a data set from the literature. Then, by numerical simulations, we find that under constant operation of the culture and constant environmental conditions (illumination, temperature, pH, etc.), solutions of the model approach towards either a positive or an extinction steady state. Based on the positive steady state, and in the context of wastewater treatment, we evaluate the capacity of microalgae to remove contaminants. We...
Tipo: Text
Palavras-chave: Control; Biotechnology; Wastewater treatment; Microalgae; Cell quota model.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00600/71188/69541.pdf

Quantitative Models for Efficient Cloning of Different Vectors with Various Clone sites
Provedor de dados: 17
Autores: Gang Zhang; Anurag Tandon.
We developed an efficient &amp;#x22;Combinatorial Strategy&amp;#x201d; for cloning different vectors with various clone sites. 1) Using originally existed clone sites from circular vectors to prepare the inserts, and if no appropriate sites are available, performing SDM to create compatible sites, to achieve maximal correct digestion of the inserts. 2) Different vectors were digested with various restriction endonucleases, and then dephosphorylated with CIP. 3) Top10 competent cells were used for transformation to increase the transformant colonies. Our results showed that, when blunt sites, or a Xba I site was adopted for ligation, the percentages of positive clones were about 50%. Whereas, when different sites, including one blunt and another Pst I...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics &amp; Genomics; Immunology; Microbiology; Molecular Cell Biology; Neuroscience.
Ano: 2012
URL: http://precedings.nature.com/documents/6965/version/1

Quantitative MRI and MR Spectroscopy of Multiple Sclerosis, Alzheimer&amp;#x2019;s Disease, Epilepsy and Cancer Microimaging Techniques
Provedor de dados: 17
Autores: Rakesh Sharma.
Clinical MRI and MRS have promise in molecular imaging. Microimaging techniques offer a promising research tool to get noninvasive data in monitoring the effect of drugs.
Tipo: Presentation
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3485/version/1

Rapid antigen detection using the liquid sample as a lens and self-mixer for light scattering detection
Provedor de dados: 17
Autores: Antonio Garcia; John Schneider.
Superhydrophobic surfaces, both natural and artificial, cause aqueous drops to bead on them. The most water-repellent surfaces produce nearly perfect spheres with very little contact between water, solutes, and surface due to the roughness of the surface ^1^. However, a related phenomenon that has not been well appreciated until recently, is that colloidal particles in the aqueous drop remain highly mobile, and smaller-sized colloids migrate to the drop surface due to both the driving force of evaporation and the expanded liquid/air interface ^2-3^. A newly identified, third useful optical property resulting from the suspension of an aqueous drop by the superhydrophobic surface is that a spheroidal lens is created. Without any additional energy or...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4754/version/1

Rapid bioassay for evaluating enzyme production in fungal isolates from environmental sources
Provedor de dados: 17
Autores: Eleonora Bordo; Francesca Luziatellie; Silvia Crognale; Sara Marinari; Stefano Grego; Maurizio Ruzzi.
Fungal hydrolytic enzymes have a great potential due to the rapid development of enzyme technology and their industrial applications. A fluorimetric microplate assay has been developed for measuring activity of &amp;#x3b2;-D-exoglucanase, &amp;#x3b2;-D-glucosidase, &amp;#x3b1;-D-glucosidase, &amp;#x3b2;-N-acetyl-hexosaminidase, &amp;#x3b2;-D-xylosidase  in diluted culture broth samples. The substrates used are conjugates of the highly fluorescent compounds 4-methylumbelliferone (MUB) and thus product formation can be measured directly in the microplate without previous extraction and purification of the product. The developed system was evaluated on eight fungal strains isolated from shrub species (Tamarix) that can be used in phytoremediation. Depending...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6217/version/1

Rapid pre-gel visualization of proteins with mass spectrometry compatibility
Provedor de dados: 17
Autores: Marco A. Mata-Gomez; Matthew T. Yasui; Robert Winkler.
Despite all of the prophecies of doom, gel electrophoresis is still prevalent in modern proteomic workflows. However, the currently used protein staining methods represent a serious bottleneck for a quick subsequent protein analysis using mass spectrometry. Substituting traditional protein stains by pre-gel derivatization with visible and mass spectrometry compatible reagents eliminates several processing steps and drastically reduces the sample preparation time. A defined chemistry permits seamless integration of such covalent protein staining methods into standardized bioinformatic pipelines. Using Uniblue A we could covalently stain simple to complex protein samples within 1 minute. Protein profiles on the gels were not compromised and MS/MS based...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/5163/version/1

Recent trends in US patent grants and issues to be considered
Provedor de dados: 17
Autores: George M. Garrity; Charles T. Parker.
The life sciences have changed radically since the Convention on Biological Diversity first opened for signatures in 1992. Traditional organism-based approaches to discovery and use of genetic resources have been supplanted by molecular approaches. Biodiversity prospecting is more likely to be a programmatic bioinformatics activity rather than an activity conducted by field scientists. Access to genetic resources is no longer centered on a hunt for novel species. Rather, the hunt is for novel genes and metabolic pathways that can be cloned into well-understood expression systems and readily scaled-up for industrial production. Information about contemporary research, development, and manufacturing practices needs to be addressed, especially when those...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4998/version/1

Recombinant Mitochondrial Transcription Factor A with N-terminal Mitochondrial Transduction Domain Increases Respiration and Mitochondrial Gene Expression in G11778A Leber&amp;#x27;s Hereditary Optic Neuropathy Cybrid Cells
Provedor de dados: 17
Autores: Shilpa Iyer; Ravindar R. Thomas; Francisco R. Portell; Lisa D. Dunahm; Caitlin K. Quigley; James P. Bennett.
Diseases involving mitochondrial defects usually manifest themselves in high-energy, post-mitotic tissues such as brain, retina, skeletal and cardiac muscle and frequently cause deficiencies in mitochondrial bioenergetics. We have developed a scalable procedure to produce recombinant human mitochondrial transcription factor A (TFAM) modified with an N-terminal protein transduction domain (PTD) and mitochondrial localization signal (MLS) that allow it to cross membranes and enter mitochondria through its &amp;#x22;mitochondrial transduction domain&amp;#x22; (MTD,=PTD+MLS). _In vitro_ studies in a classic mitochondrial disease cell model demonstrated that Alexa488-labeled MTD-TFAM rapidly entered the mitochondrial compartment. MTD-TFAM treatment of these...
Tipo: Manuscript
Palavras-chave: Biotechnology; Neuroscience.
Ano: 2008
URL: http://precedings.nature.com/documents/2084/version/1

Reductive Biotransformation of Ethyl Acetoacetate: A Comparative Studies using Free and Immobilized Whole Yeast Cells
Provedor de dados: 17
Autores: Amit  R. Kumbhar; Vaibhav V.  Kulkarni; Madhav B. Pande; Sachin S. Laddha.
Bioreduction of ethyl acetoacetate with free and immobilized yeast whole cell was achieved by using water and sucrose combination. After detachment from immobilized beads under basic condition, the corresponding ethyl(S)-(+)-3-hydroxybutanoate was isolated with 98 to 100% yield. Immobilized beads of yeast whole cell were prepared at different temperature which affects the morphology and physiology of the beads for the diffusion of the enzyme, which shown the maximum conversion of the substrate to products as compared to the free yeast whole cell.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/2087/version/1

Reflect: Augmented Browsing for the Life Scientist
Provedor de dados: 17
Autores: Evangelos Pafilis; Se&amp;#xe1;n I. O&amp;#x27;Donoghue; Lars J. Jensen; Heiko Horn; Michael Kuhn; Nigel P. Brown; Reinhard Schneider.
Anyone who regularly reads life science literature often comes across names of genes, proteins, or small molecules that they would like to know more about. To make this process easier, we have developed a new, free service called Reflect (&amp;#x22;http://reflect.ws&amp;#x22;:http://reflect.ws) that can be installed as a plug-in to Firefox or Internet Explorer. Reflect tags gene, protein, and small molecule names in any web page, typically within a few seconds, and without affecting document layout. Clicking on a tagged gene or protein name opens a popup showing a concise summary that includes synonyms, database identifiers, sequence, domains, 3D structure, interaction partners, subcellular location, and related literature. Clicking on a tagged small...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3212/version/1

Reguladores de crescimento e época de inoculação dos óvulos no resgate de embriões de uvas sem sementes.
Provedor de dados: 14
Autores: NUNES, B. T. G.; MELO, N. F. de; LEAO, P. C. de S.
O presente estudo teve por objetivo avaliar a aplicação de regulares de crescimento e da época de coleta e inoculação in vitro de óvulos de videira em diferentes cruzamentos sobre a percentagem e número de embriões resgatados e germinados. Dois experimentos foram realizados na Embrapa Semiárido, Petrolina, PE. No primeiro experimento os tratamentos foram representados pelos reguladores de crescimento ANA, BAP, GA3 e a combinação ANA + BAP e BAP + GA3 aplicados aos 10 dias antes da floração e 7 dias após a polinização. No segundo experimento, foram avaliadas três épocas de coleta e inoculação dos óvulos (6, 7 e 8 semanas após a polinização). Os resultados obtidos permitem concluir que as respostas variam em função dos genótipos parentais, mas a pulverização...
Tipo: Parte de livro
Palavras-chave: Uva sem semente; Inoculação in vitro de óvulos de videira; Melhoramento Genético Vegetal; Uva; Biotecnologia; Regulador de Crescimento; Grapes; Biotechnology; Growth regulators.
Ano: 2022
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1144433

Regulating Nanomedicine: A European perspective
Provedor de dados: 17
Autores: Joel D&amp;#x27;Silva; Geert Van Calster.
The potential impact of nanomedicine is foreseen to radically change health care; however it also challenges existing perceptions, dynamics and standards relating to ethics, safety and governance. This commentary briefly highlights aspects and approaches pertaining to nanomedical regulation in the European Union.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2388/version/1

Regulatory Approval Decisions in the Presence of Market Externalities: The Case of Genetically Modified Wheat
Provedor de dados: 31
Autores: Furtan,   William Hartley; Gray,   Richard S.; Holzman,   J.J.
This study examines the optimal approval strategy for genetically modified (GM) wheat varieties in Canada and the United States. Without an affordable segregation system, the introduction of GM wheat will create a market for "lemons" that will result in the loss of important export markets. Using a differentiated product trade model for spring wheat, with endogenous technology pricing, a payoff matrix is generated for the possible approval outcomes. Results show that the existence of the market externality removes the first-mover advantage for wheat producers from the approval of the new GM wheat variety. There are large distributional effects; wheat producers lose economic surplus, while consumers and the biotech company gain economic surplus. With a...
Tipo: Journal Article
Palavras-chave: Biotechnology; Market externalities; Non cooperative games; Strategic approval decisions; Trade; Agricultural and Food Policy.
Ano: 2005
URL: http://purl.umn.edu/30778

Relationship of arterial and exhaled CO2 during elevated artificial pneumoperitoneum pressure for introduction of the first trocar.
Provedor de dados: 17
Autores: Octavio H. M. Hypolito; Joao-Luiz Azevedo; Susana A. Miyahira; Fabiana Scarpelli; Otavio M. Becker Junior; Afonso   C. C. G. Machado; Otavio C. Azevedo; Glicia C. Azevedo; Bianca Marigliani; Jose F. Borborema; Linda  Bernardes; Gustavo P. S. Miguel.
The present study evaluated the correlation between arterial CO2  and exhaled CO2 during brief high-pressure pneumoperitoneum. Patients were randomly distributed into two groups:  P12 group (n=30) received a maximum intraperitoneal pressure of 12mmHg, and P20 group (n=37) received a maximum intraperitoneal pressure of 20mmHg. Arterial CO2 was evaluated by radial arterial catheter and exhaled CO2 was measured by capnometry at the following time points: before insufflation, once intraperitoneal pressure reached 12mmHg , 5 minutes after intraperitoneal pressure reached 12mmHg for the P12 group or 20mmHg for the P20 group,  and 10 minutes after intraperitoneal pressure reached 12mmHg for the P12 group or when intraperitoneal pressure  had decreased from 20mmHg...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6538/version/1

Research need assessment and agricultural research priorities for South and West Asia
Provedor de dados: 30
Autores: M.C.S.
Palavras-chave: Agroclimatic zones; Biotechnology; Policies; Agroecosystems; Rice; Agroecology; Economic indicators; Postharvest technology; Grain legumes; Capacity building.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4237

Resumo dos trabalhos...
Provedor de dados: 14
Autores: TALENTO ESTUDANTIL DO CENARGEN, 1996, Brasília, DF.
A evolução dos mapas genéticos; Influência da temperatura de cultivo no crescimento e toxicidade do isolado S93 de Bacillus thuringiensis supesp. Kurstaki; Efeito da previsibilidade alimentar no vigor da prole de Podisum nigrispinus; Diagnóstico molecular para uso e preservação de germoplasma equino.
Tipo: Livros
Palavras-chave: Biology; Genetic; Biologia; Biotecnologia; Controle Biológico; Genética; Biotechnology.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/171474

Resumos...
Provedor de dados: 14
Autores: ENCONTRO DE PRODUÇÃO CIENTÍFICA DA EMBRAPA ALGODÃO, 28., 2021, Campina Grande, PB.
Formar pessoas capazes de buscar conhecimentos por meio da pesquisa e saber utilizá-los em benefício da sociedade. Este é um dos grandes desafios que resume a importância do Programa Institucional de Bolsas de Iniciação Científica do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/Pibic) para estudantes de graduação nas universidades, em parceria com institutos de pesquisa. É dentro desta perspectiva que o Encontro de Produção Científica (EPC) se insere como uma etapa obrigatória do processo formal de avaliação dos bolsistas do Programa, enquanto orientandos dos pesquisadores da Embrapa Algodão. Anualmente, o EPC proporciona a estudantes iniciantes na pesquisa a participação em um evento de natureza científica, em que são apresentados...
Tipo: Anais e Proceedings de eventos
Palavras-chave: Algodão; Pesquisa Agrícola; Agricultura; Tecnologia; Controle Químico; Herbicida; Soja; Milho; Cotton; Research; Chemical control; No-tillage; Soybeans; Biotechnology; Herbicides; Agriculture.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1138090

Resveratrol Increases Intracellular NAD+ Levels Through Up regulation of The NAD+ Synthetic Enzyme Nicotinamide Mononucleotide Adenylyltransferase
Provedor de dados: 17
Autores: Ross S. Grant.
Resveratrol is a polyphenol with major health benefits that is thought to operate through direct activation of the &amp;#x27;anti-aging&amp;#x27; enzyme SIRT1. However recent reports have challenged this &amp;#x27;direct-activation&amp;#x27; hypothesis, suggesting the mechanism by which resveratrol increases SIRT1 function is still unknown. We report for the first time that resveratrol induces a dose dependent increase in activity of the NAD+ synthetic enzyme nicotinamide mononucleotide adenylyl transferase (NMNAT1). Activation of NMNAT1 by resveratrol in cultured primary human astrocytes and neurons increased NAD+ levels by up to 5 fold. As SIRT1 requires NAD+ as a substrate to perform its gene silencing function, higher NAD+ levels will enhance SIRT1...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4421/version/1

Reversal of an immunity associated plant cell death program by the growth regulator auxin
Provedor de dados: 17
Autores: Suresh Gopalan.
One form of plant immunity against pathogens involves a rapid host programmed cell death at the site of infection accompanied by resistance, termed the hypersensitive response (HR). Here it is shown that the HR programmed cell death program initiated by the bacterial type III secretion system dependent proteinaceous elicitor harpin from Erwinia amylovora can be reversed till very late in the process by the plant growth regulator auxin. Early inhibition or late reversal of this cell death program does not affect marker genes tightly correlated with local and systemic resistance. Cross-regulation between cell death programs and growth regulators is prevalent in different kingdoms. Thus, the concept that cell death program can be reversed till late provides a...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Molecular Cell Biology; Plant Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/1347/version/1

RGDU06337-MAS-24-B-B: ข้าวเจ้าอายุเบาสำหรับนาน้ำฝนภาคตะวันออกเฉียงเหนือ
Provedor de dados: 106
Autores: Somjai Saleeto.
The program on research and development of rainfed lowland rice in north eastern Thailand in collaboration with the National Centre for Genetic Engineering and Biotechnology (BIOTEC) have carried out the project to develop an early maturing, blast resistant, non-glutinous rice variety. The cross between IR57514-PMI-5-B-1-2 and RGDU99016-MAS-77 was developed and the progenies were backcrossed to IR57514-PMI-5-B-1-2 for 3 time at RGDU, BIOTEC. The selected promising line; RGDU06337-MAS-24-B-B, was finally obtained. This line is a non-glutinous, photoperiod sensitive, early maturing line which flowers approximately on October 8 and is ready for harvest on November 11. The average number of panicles per hill is 11 panicles. The average plant height is 114 cm....
Tipo: PhysicalObject
Palavras-chave: Rice; Non-glutinous rice; Rainfed lowland rice; Semi dwarf; Blast; Blast resistance; Early maturing rice; North-eastern region; Biotechnology; Genetic engineering; ข้าว; ข้าวนาน้ำฝน; ต้นเตี้ย; โรคไหม้; ความต้านทานต่อโรคไหม้; ข้าวเบา; ข้าวอายุสั้น; ภาคตะวันออกเฉียงเหนือ; เทคโนโลยีชีวภาพ; พันธุวิศวกรรม.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5631

Risk assessment and management of genetically modified organisms under Australia's Gene Technology Act
Provedor de dados: 31
Autores: Linacre,   Nicholas A.; Falck-Zepeda,   Jose Benjamin; Komen,   John; MacLaren,   Donald.
Compared to both Canada and the United States, Australia has been slow to approve commercial planting of transgenic crops. Two probable reasons exist for the slow approval rate of transgenic crops in Australia. The first reason is community perceptions about the risks associated with transgenic technologies. The second is the regulatory framework currently employed to approve commercial releases. This paper examines some of the potential regulatory issues that may be affecting the review process and approval of transgenic technologies. First we provide a brief introduction to the regulatory structure in Australia, second we consider the impact of regional, national and state jurisdictions, third we argue that the regulator needs to consider the use of...
Tipo: Report
Palavras-chave: Risk assessment; Biotechnology; Environmental risk; Crop Production/Industries.
Ano: 2006
URL: http://purl.umn.edu/55414

Riverina high school students’ views of biotechnology
Provedor de dados: 69
Autores: Cavanagh,Heather; Hood,Jennie; Wilkinson,Jenny.
A survey investigating the knowledge and perception of biotechnology by high school students living in the rural Riverina region of New South Wales, Australia, was undertaken. Data relating to the student’ preferred and trusted information sources was also gathered. This study has demonstrated that at least two-thirds of students had a good knowledge of medical biotechnology issues, however, a significant proportion of the students did have concerns about the use and/or safety of biotechnology. Nearly 90% of the respondents would like further information on biotechnology. In agreement with other surveys, the students reported an apparent lack of trust in articles from the lay press (newspapers, television, radio) and yet still report that these media as...
Tipo: Journal article
Palavras-chave: Biotechnology; Education; Science; Secondary school.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200001

Robustness of self-organised systems to changes in individual level behaviour: an example from real and simulated self-organised snail aggregations
Provedor de dados: 17
Autores: Richard Stafford; Mark S. Davies; Gray A. Williams.
Perfect behaviours that are optimal to the environment an agent operates within rarely exist in real animals or in robotic systems. The costs (be they biological or economic) of building sensors and processing the information they capture become excessive compared to the small advantages that occur from the modifications of behaviour. Many self-organised systems are thought to change their properties as a result of changes in individual behaviour. Here, using both natural systems and computer simulations, we demonstrate that intertidal snail aggregations slightly decrease in size when individuals forage for shorter periods due to hotter and more desiccating conditions &amp;#x2013; a non-optimal behaviour for the snails since aggregation reduces desiccation...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Evolutionary Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3922/version/1

Role of intellectual property rights in biotechnology and pharmaceutical industries
Provedor de dados: 17
Autores: Ajeet Ajeet.
The main objective of this review is to highlight and explore the inter-relationship and the functioning of the intellectual property right in the pharmaceutical and biotechnology industry. The rising tide of patent applications can be witnessed globally in this industry as the need for such protection and licensing has become imperative so as to safeguard the rights of the inventor and also to encourage and promote new talents, inventions and innovations which can be a boon for the economy. The field of biotechnology is an upcoming science which is still at the initial stage of establishing  a foundation but it promises a revolution in the fields of  medicine, agriculture, pharmaceuticals and industrial sector amongst  other sectors of the economy along...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/7065/version/1

Role of the porous structure of the bioceramic scaffolds in bone tissue engineering
Provedor de dados: 17
Autores: Wang Zhen; Chang Jiang; Bai Feng; Sun Xiaojiang; Lu Jianxi; Lin Li; Lei Chen; Dai Rong.
The porous structure of biomaterials plays a critical role in improving the efficiency of biomaterials in tissue engineering. Here we fabricate successfully porous bioceramics with accurately controlled pore parameters, and investigate the effect of pore parameters on the mechanical property, the cell seeding proliferation and the vascularization of the scaffolds. This study shows that the porosity play an important role on the mechanical property of the scaffolds, which is affected not only by the macropores size, but also by the interconnections of the scaffolds. Larger pores are beneficial for cell growth in scaffolds. In contrast, the interconnections do not affect cell growth much. The interconnections appear to limit the number of blood vessels...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4148/version/1

Rural Poverty and Employment Effects of Bt Cotton in India
Provedor de dados: 31
Autores: Subramanian,   Arjunan; Qaim,   Matin.
The impact of genetically modified (GM) crops on the poor in developing countries is still the subject of controversy. While previous studies have examined direct productivity effects of Bacillus thuringiensis (Bt) cotton and other GM crops, little is known about wider socioeconomic outcomes. We use a microeconomic modelling approach and comprehensive survey data from India to analyze welfare and distribution effects in a typical village economy. Bt cotton adoption increases aggregate employment with interesting gender implications. Likewise, aggregate household incomes rise, including for poor and vulnerable farmers, highlighting that Bt cotton contributes to poverty reduction and rural development.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Genetically modified crops; Poverty; Rural development; Village economy; Social accounting matrix; Impacts; Agricultural and Food Policy; Community/Rural/Urban Development; Crop Production/Industries; Food Security and Poverty; International Development; Production Economics; Research and Development/Tech Change/Emerging Technologies.
Ano: 2009
URL: http://purl.umn.edu/50555

SABIO-RK: Access to Biochemical Reaction Kinetics Data
Provedor de dados: 17
Autores: Martin Golebiewski.
SABIO-RK (http://sabio.h-its.org/) is a curated database for biochemical reaction kinetics data drawn from different sources: from literature or direct submission by experimenters. The system offers standardized data by the use of controlled vocabularies and annotations pointing to other resources and biological ontologies. SABIO-RK can be accessed either manually via a web-based search interface or automatically via web services that allow direct data access by other tools. Both interfaces support the export of the data together with its annotations in SBML (Systems Biology Markup Language) complying with the MIRIAM (Minimal Information Requested In the Annotation of biochemical Models) standard. New RESTful web services, as well as a new browser...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6506/version/1

Safety and Socio-Economic Issues Raised by Modern Biotechnology
Provedor de dados: 31
Autores: Xue,   Dayuan; Tisdell,   Clement A.
Modern new biotechnology has the potential to provide major economic and other benefits, but at the same time it poses potential hazards for human health, the environment, the ‘natural’ biological order and can have adverse socio-economic consequences.  The application of such technology frequently violates traditional ethical, moral and religious values.  This paper after outlining possible benefits of modern new biotechnologies, discusses the type of biosafety risks which they pose, their possible adverse consequences for the sustainability of biodiversity and agriculture and their potential impacts on socioeconomic welfare and traditional cultures.  Particular concern is expressed about the possible consequences of such technologies for developing...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Socio-economic policy; Genetic engineering; Biodiversity; Biosafety; Patents; Environmental Economics and Policy; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/47995

Salmonella typhimurium harboring plasmid expressing interleukin-12 induced attenuation of infection and protective immune responses
Provedor de dados: 17
Autores: Yong Keun Park; Yoon Won Suck; Lee Seung Seok.
IL-12 is known to be an essential cytokine which appears to provide protective immunity against intracellular bacteria, such as Salmonella. In this study, we investigated the possibility of developing a vaccine using IL-12 against virulent Salmonella. We used the host defense system activated by cytokine IL-12. The highly virulent Salmonella strain (Salmonella typhimurium UK-1) was transformed with cytokine-expressing plasmids. These live, wild-type pathogens were used as vaccine strains without undergoing any other biological or genetic attenuating processes. The newly developed strains induced partial protection from infections (30-40%). Of note, the interleukin-12 transformed pathogen was safe upon immunization with low doses (103 CFU), induced IgG...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3413/version/1

SBGN-ED - a tool for editing, validating, and translating of SBGN maps
Provedor de dados: 17
Autores: Tobias Czauderna; Christian Klukas; Astrid Junker; Falk Schreiber.
The recently proposed Systems Biology Graphical Notation (SBGN) provides a standard for the visual representation of biochemical and cellular processes. SBGN helps to communicate biological knowledge more efficient and accurate between different research communities in the life sciences. However, to support SBGN, methods and tools for editing, validating, and translating of SBGN maps are necessary.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;We present methods for these tasks, the tool SBGN-ED, and an application example. SBGN-ED allows to create all three types of SBGN maps from scratch, to validate these maps for syntactical and semantical correctness, to translate maps from the KEGG and MetaCrop databases into SBGN, and to export SBGN maps into several file and...
Tipo: Poster
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4973/version/1

SBML Level 3 Hierarchical Model Composition
Provedor de dados: 17
Autores: Lucian P. Smith; Michael Hucka.
A new proposal for incorporating modularity (or &amp;#x27;model composition&amp;#x27;) into SBML Level 3.
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5133/version/1

Scenarios, challenges and opportunities for sustainable agricultural chemistry.
Provedor de dados: 14
Autores: VAZ JUNIOR, S.
bitstream/item/202219/1/SILVIODOC-30.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Sustainability; Agrochemicals; Biotechnology.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1112377

Scientific progress in the production of monogastric in the first decade of the twenty-first century
Provedor de dados: 96
Autores: Costa,Fernando Guilherme Perazzo; Silva,José Humberto Vilar da; Lima,Raffaella Castro de; Oliveira,Cleber Franklin Santos de; Rodrigues,Valéria Pereira; Pinheiro,Sarah Gomes.
Significant progress in the area of animal production is due to the development of researches on genetics, environment, health, management and nutrition. The search for better management conditions that benefit animal welfare resulted in changes to the installation. The genetic improvement more productive animals has encouraged the number of searches to reduce the incidence of diseases, mainly of metabolic origin. The incorporation in products with higher bioavailability as industrial amino acid and organic minerals, the inclusion of products modifiers enteric of microflora as probiotic, prebiotic, symbiotic, nucleotides and exogenous enzymes, has benefited the use of nutrients are not well absorbed and often unavailable in the diet. Biotechnology latest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Biotechnology; Nutrition; Phenomenal progress.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300032

Screening Lactic Acid Bacteria for Antimicrobial Compound Production
Provedor de dados: 17
Autores: Khalisanni Khalid; Lee  Hung Kiong.
Lactic Acid Bacteria was known as potential probiotic used in food industries and dairy products and probable to produce antimicrobial compound that inhibit variety of microorganisms. The objectives of the research are to determine the optimum condition and glucose utilization in relation to antimicrobial compound production. Two species of Lactic Acid Bacteria namely Lactococcus and Lactobacillus were used as probiotic. The Lactic Acid Bacteria were fermentated in different medium, initial substrate pH and incubation temperature for the production of antimicrobial compound. The test organisms such as E.coli and Salmonella were selected as test organisms. Amongst the two species of Lactic Acid Bacteria, Lactococcus produced the highest amount of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/3326/version/1

Screening of actinobacteria for antimicrobial activities by a modified &amp;#x22;Cross-Streak&amp;#x22; method
Provedor de dados: 17
Autores: Nandkumar Kamat; Sonashia Velho-Pereira.
New molecule discovery from natural sources, such as that of actinobacteria, has proved to be an interesting area in antibiotic research, as most of these antibiotics are difficult to synthesize. Out of 30 actinobacterial cultures screened for antimicrobial activity, 28 cultures were found to produce active products against various pathogenic microorganisms such as Gram-negative, Gram-positive bacteria and yeast, using a &amp;#x2018;modified cross streak method.&amp;#x27; The modified method helped in easy quantification of results and also in ruling out probable mutual antibiosis. 53%, 13% and 10% of tested actinobacterial strains belonging to Streptomyces, Micromonospora and Actinomadura genera, respectively, showed the ability of producing antimicrobial...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Immunology; Microbiology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6765/version/1

Screening Of Antibiotic Producing Microorganism From Rhizoshphere Soil, Their Antibiotic Production And Characterization
Provedor de dados: 17
Autores: Vishwambhar V. Bhandare; Sandip Mahadev Chavre.
Rhizosphere soil samples were preferred as it contains most of the prospective antibiotic producers. In an attempt to screen out new potent antibiotic producers from soil, the Rhizobium species was isolated. Antibiotic produced by shake flask culture is found to be effective against both gram positive and gram negative organisms. This product was found to be a very promising antibiotic when assayed biologically. UV treatment showed positive effect on antibiotic production. The molecular structure of the antibiotic was identified by using advanced analytical techniques like UV Visible spectroscopy, FTIR, GCMS, NMR spectroscopy.
Tipo: Poster
Palavras-chave: Biotechnology; Microbiology.
Ano: 2011
URL: http://precedings.nature.com/documents/6101/version/1

Secondary water pore formation for proton transport in a ClC exchanger revealed by an atomistic molecular dynamics simulation
Provedor de dados: 17
Autores: Youn Jo Ko; Won Ho Jo.
Several prokaryotic ClC proteins have been demonstrated to function as exchangers that transport both chloride ions and protons simultaneously in opposite directions. However, the path of the proton through the ClC exchanger and how the protein brings about the coupled movement of both ions are still unknown. In the present work, we demonstrate that a previously unknown secondary water pore is formed inside a ClC exchanger by using an atomistic molecular dynamics (MD) simulation. From the systematic simulations, it was determined that the glutamate residue exposed to the intracellular solution, E203, plays an important role as a trigger for the formation of the secondary water pore. Based on our simulation results, we conclude that protons in the ClC...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/2168/version/1

Seed technology training in the year 2000
Provedor de dados: 63
Autores: McDonald,M.B.; Marcos-Filho,J.; Bino,R.J.
Seed quality will remain the centerpiece of successful agricultural programs in the year 2000. As new changes occur in agriculture driven by advancements in biotechnology, seed enhancement technologies, a more diverse seed user clientele, and communication technologies, successful seed companies will require a knowledgeable and informed workforce to assure high seed quality. A new approach to seed technology training is professed that relies on the establishment of a three-institution consortium to achieve this objective. Advantages of the consortium are identified that emphasize the unique strengths of each institution, their geographic advantages representing major climactic/agricultural zones in the world, and differing approaches to seed technology...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seed quality; Education; Seed industry; Biotechnology; Seed enhancements; Consortium.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500001

Seeking a Common Path: Structuring Multistakeholder Dialogue on Agricultural Biotechnology in Africa
Provedor de dados: 31
Autores: von Grebmer,   Klaus; Omamo,   Steven Were.
This paper analyzes an ongoing effort by national, regional, and international partners to raise awareness, promote dialogue, and catalyze consensus-building mechanisms among stakeholder groups on the role of biotechnology in agricultural development and food security in southern Africa. Stakeholders include public bodies, the private sector, and civil society. The paper argues that while responsible adoption of agricultural biotechnology promises significant gains to the countries of southern Africa, governments must clarify its specific role, improve policies for its application, and assess the place of biotechnology in broader development strategies. The issues that surround the introduction, creation, and application of agricultural biotechnology in...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Consensus-building; Conflict resolution; Africa; Food security; Genetically modified food; Biosafety; Capacity building; Research and Development/Tech Change/Emerging Technologies.
Ano: 2006
URL: http://purl.umn.edu/58581

Selection of somaclonal variants of the cultivar ‘Prata-Anã’ for resistance to Fusarium oxysporum f. sp. cubense race 1
Provedor de dados: 94
Autores: Ferreira,Mileide dos Santos; Moura,Érica Rodrigues de; Lino,Lucymeire Souza Morais; Amorim,Edson Perito; Santos-Serejo,Janay Almeida dos; Haddad,Fernando.
Abstract  The banana tree is one of the most cultivated fruit globally; however, some diseases significantly affect its production, such as Fusarium wilt. The most appropriate measure for controlling this disease in areas with inoculum pressure is the use of resistant cultivars. Therefore, this study aimed to generate banana somaclones of the cultivar ‘Prata-Anã’ resistant to Fusarium wilt by inducing somaclonal variation. ‘Prata-Anã’ stem apexes were established in vitro in MS culture medium and, on a monthly basis, subcultivated in AIA and adenine sulfate supplemented MS medium with added plant regulators: 6-benzylaminopurine (BAP, 4 ml L-1), Thidiazuron (TDZ, 1 ml L-1), and Paclobutrazol (PBZ, 10 ml L-1). The treatments were: T0: no regulator, T1: BAP,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Tissue culture; Musa sp.; Somaclonal variations; Fusarium wilt.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452020000600101

Sequence-specific Solution Structures of the Four Isosequential Pairs of Single-stranded DNAs and RNAs
Provedor de dados: 17
Autores: Subhrangsu  Chatterjee; Wimal  Pathmasiri; Jyoti Chattopadhyaya.
The role of the sequence-context in the self-organization of four single-stranded (ss) isosequential pairs of DNAs (1 &amp;#x2013; 4) and RNAs (5 &amp;#x2013; 8), [d/r-(5&amp;#x27;C^1^A^2^X^3^G^4^Y^5^A^6^C^7^): X^3^ = A or C, Y^5^ = A or C; sequence variations: 2^2^ = 4], has been elucidated by NMR-constrained Molecular Dynamics (MD) simulations (2 ns). Following sequence-specific observations have been made from the solution NMR and the NMR constrained MD simulation study: (i) Analysis of the NOESY footprints, mainly (H8/H6)~n~ to (H1&amp;#x27; and H3&amp;#x27;)~n-1~ contacts, of ssDNAs (1 - 4) and ssRNAs (5 &amp;#x2013; 8) in the aqueous medium have shown that all ssDNAs (1 - 4) and ssRNAs (5 - 8) adopt right handed stacked helical structures in the NMR...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1685/version/1

Serine biosynthesis with one carbon catabolism represents a novel pathway for ATP generation in cells using alternative glycolysis with zero net ATP production
Provedor de dados: 17
Autores: Alexei Vazquez; Zoltan N. Oltvai.
Recent experimental evidence indicates that some cancer cells have an alternative glycolysis pathway with net zero ATP production, implying that upregulation of glycolysis in these cells may not be related to the generation of ATP. Here we use a genome-scale model of human cell metabolism to investigate the potential metabolic alterations in cells using net zero ATP glycolysis. We uncover a novel pathway for ATP generation that involves reactions from the serine biosynthesis and one-carbon metabolism pathways. This pathway has a predicted two-fold higher flux rate in cells using net zero ATP glycolysis than those using standard glycolysis and generates twice as much ATP with significantly lower rate of lactate- but higher rate of alanine secretion. Thus,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6123/version/1

Serine biosynthesis with one carbon catabolism represents a novel pathway for ATP generation in cells using alternative glycolysis with zero net ATP production
Provedor de dados: 17
Autores: Alexei Vazquez; Elke K. Markert; Zoltan N. Oltvai.
Recent experimental evidence indicates that some cancer cells have an alternative glycolysis pathway with net zero ATP production, implying that upregulation of glycolysis in these cells may not be related to the generation of ATP. Here we use a genome-scale model of human cell metabolism to investigate the potential metabolic alterations in cells using net zero ATP glycolysis. We uncover a novel pathway for ATP generation that involves reactions from the serine biosynthesis and one-carbon metabolism pathways. This pathway has a predicted two-fold higher flux rate in cells using net zero ATP glycolysis than those using standard glycolysis and generates twice as much ATP with significantly lower rate of lactate- but higher rate of alanine secretion. Thus,...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6123/version/2

Sharing perspectives on public-private sector interaction
Provedor de dados: 30
Autores: A.J.
Palavras-chave: Private sector; Hybrids; Policies; Biotechnology; Genetic resources; Breeds (animals); Millets; Agreements; Patents; Sugarcane.
Ano: 2001
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4226

Signatures of spatial organizations &amp;#x2013; From the universe of proteins to the universe in general
Provedor de dados: 17
Autores: Aditya Mittal.
Pattern generation and recognition in 3-D space have been of wide interest in fields varying from atomic and molecular structures to organization of celestial objects. Our thoughts towards investigating patterns (and elucidation of geometrical rules) followed by a known number of points with specific or specified coordinates are guided by the assumption/belief that there must be some thermodynamic and/or kinetic basis leading to the organization of coordinates of the known points. In terms of biomolecular structures, the DNA double helix is one of the strongest examples of such structures with coordinates of atoms in the phosphate backbone and the side-chains provide a helical geometrical configuration. It needs to be appreciated (and emphasized) that,...
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6038/version/1

Simcluster: clustering enumeration gene expression data on the simplex space
Provedor de dados: 17
Autores: Ricardo Z. N. V&amp;#xea;ncio; Leonardo A. B. Varuzza; Carlos Pereira; Helena Brentani; Ilya Shmulevich.
Transcript enumeration methods such as SAGE, MPSS, and sequencing-by-synthesis EST &amp;#x22;digital northern&amp;#x22;, are important high-throughput techniques for digital gene expression measurement. As other counting or voting processes, these measurements constitute compositional data exhibiting properties particular to the simplex space where the summation of the components is constrained. These properties are not present on regular Euclidean spaces, on which hybridization-based microarray data is often modeled. Therefore, pattern recognition methods commonly used for microarray data analysis may be non-informative for the data generated by transcript enumeration techniques since they ignore certain fundamental properties of this...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/202/version/1

Similaridade genetica de acessos de arachis pintoi com diferentes niveis de produtividade de materia seca com base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; ANDRADE, R. P.; KARIA, C. T. CORDEIRO, M. C. R.; SOUZA, T. L. P. O.; MERIDA, J. L.
bitstream/CPAC-2009/24947/1/p2004_01.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: RAPD; Peanut; Feed crops.; Amendoim; Biotecnologia; Marcador Molecular; Planta Forrageira; Variação Genética.; Genetic variation.; Genetic markers; Biotechnology.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/559244

Simple reverse genetics approach to elucidating the biosynthetic pathway of complex thiopeptide nocathiacin
Provedor de dados: 17
Autores: Maochen Wei; Jing Deng; Shuzhen Wang; Nan Liu; Yijun Chen.
Biothythetic pathway of the most drugable thiopeptide nocathiacin has been elucidated by applying reverse genetics method based on its structural features. The present study provides an efficient approach for an easy access to the biosynthetic gene clusters of complex bioactive peptides that are ribosomally synthesized with extensive posttranslational modifications.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4740/version/1

Simulation of Two Dimensional Ultraviolet (2DUV) Spectroscopy of Amyloid Fibrils
Provedor de dados: 17
Autores: Jun Jiang; Darius Abramavicius; Cyril Falvo; Benjamin Bulheller; Jonathan Hirst; Shaul Mukamel.
Revealing the structure and aggregation mechanism of amyloid fibrils is essential for the treatment of over 20 diseases related to protein misfolding. Coherent two dimensional (2D) infrared spectroscopy is a novel tool that provides a wealth of new insight into the structure and dynamics of biomolecular systems. Recently developed ultrafast laser sources are extending multidimensional spectroscopy into the ultraviolet (UV), and this opens up new opportunities for probing fibrils. In a simulation study, we show that 2DUV spectra of the backbone of a 32-residue &amp;#x3b2;-amyloid (A&amp;#x3b2;(9-40)) fibril associated with Alzheimer&amp;#x27;s disease, and two intermediate prefibrillar structures carry characteristic signatures of fibril size and geometry...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/4369/version/1

Single Cancer Cell Detection by Near Infrared Microspectroscopy, Infrared Chemical Imaging and Fluorescence Microspectroscopy
Provedor de dados: 17
Autores: I. C. Baianu; Schuyler S. Korban; Doina M. Costescu.
Novel techniques are currently being developed and established for the accurate chemical analysis and detection of single cancer cells, single embryos and single seeds by Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fourier Transform Infrared (FT-IR), Fluorescence and High-Resolution NMR (HR-NMR). The first FT-NIR chemical images of biological systems approaching 1micron resolution are here reported. 400 and 500 MHz, H-1 NMR analyses were carried out that allowed the selection of mutagenized embryos. Detailed chemical analyses are being demonstrated to be also possible by FT-NIR Chemical Imaging/ Microspectroscopy of single cancer cells. FT-NIR Microspectroscopy and Chemical Imaging are also shown to be potentially important in Functional...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Molecular Cell Biology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6207/version/1

Single cell fertilizer (SCF): Evidence to prove that bio-molecules are potent nutrient for plant growth
Provedor de dados: 17
Autores: Sribir Sen; Amit  Kr. Roy; Sayan Choudhury; Shibu Ghosh; Somenath Das; Sohini Roy; Rajib Ghosh; Dipankar Dey; Deben Pathak; Sanjeev Upadhyay; Isita Chattopadhya.
Fertilizers of various kinds are used for the cultivation of crop plants for hyper production of plant based food materials. The study used bio-molecules made in a bacterial cell. The experimental results showed tremendous effect on plant growth. These cellular molecules were made by treating the bacterial cells with   lysozyme and protenase K. The   wet/weight was increased in multiple folds compared to that of control sets. The fold of increase was 4.79 for rice, 2.77 for wheat, 1.89 for gram and 1.89 for pea when bacterial cellular molecules were used as fertilizer.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2816/version/1

Sistema de Informação da Rede Genômica Animal - Sirga: manual do usuário.
Provedor de dados: 14
Autores: HIGA, R. H.; LONG, C. K.
O software Sirga baseia-se em uma plataforma de banco de dados relacional e acesso via web, tal que o sistema pode ser acessado de qualquer local que tenha acesso à internet. Este documento apresenta os pré-requisitos necessários para utilização do sistema e uma descrição de suas funcionalidades.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Software Sirga; Microarranjos.; Biotecnologia.; Computer software; Microarray technology; Biotechnology.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/920174

Site-Specific Modification Of Proteins Mediated By Transglutaminase
Provedor de dados: 17
Autores: Abhijeet Satwekar.
Transglutaminase (TGase) catalyzes an acyl transfer reaction between the [gamma]-carboxamide group of glutamine (Gln) and the [epsilon]-amino group of lysine (Lys) residues to form a stable amide bond. The TGase reaction can be used for bioconjugation of an amino-derivative of poly-ethylene glycol (PEG) to protein drugs, leading to PEGylated proteins that display increased bioactivity and stability. The procedure was shown to lead to site-specific bioconjugation of few proteins, thus offering a valid alternative to the chemical methods of PEGylation in current use (1). Moreover, TGase can be used for site-specifically labeling proteins with fluorescent groups at the level of Gln or Lys for diagnostic applications. The TGase attack is not entirely dependent...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry.
Ano: 2011
URL: http://precedings.nature.com/documents/6000/version/1

Skin Typing and High Resolution MRI of Human Skin: Multiple Contrast Approach for Image Characterization
Provedor de dados: 17
Autores: Rakesh R. Sharma.
Background and objectives:    &amp;#xd;&amp;#xa;The ex vivo magnetic resonance microimaging (MRM) image characteristics are reported in human skin excised samples. &amp;#xd;&amp;#xa;Design and methods:&amp;#xd;&amp;#xa;Human excised skin samples were imaged using custom coil placed inside 500 MHz NMR imager for high resolution microimaging. Skin in vivo- and ex vivo MRI images were processed for characterization of different skin structures. Contiguous cross-sectional T1-weighted 3D spin echo MRI, T2-weighted 3D spin echo MRI and proton density images were compared with skin histopathology and NMR peaks. In all skin specimens, epidermis and dermis thickening and hair follicle size was measured using MRM.  &amp;#xd;&amp;#xa;Results:...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4161/version/1

Skin Typing and High Resolution MRI of Human Skin: Multiple Contrast Approach for Image Characterization
Provedor de dados: 17
Autores: Rakesh R. Sharma.
Background and objectives:    &amp;#xd;&amp;#xa;The ex vivo magnetic resonance microimaging (MRM) image characteristics are reported in human skin excised samples. &amp;#xd;&amp;#xa;Design and methods:&amp;#xd;&amp;#xa;Human excised skin samples were imaged using custom coil placed inside 500 MHz NMR imager for high resolution microimaging. Skin in vivo- and ex vivo MRI images were processed for characterization of different skin structures. Contiguous cross-sectional T1-weighted 3D spin echo MRI, T2-weighted 3D spin echo MRI and proton density images were compared with skin histopathology and NMR peaks. In all skin specimens, epidermis and dermis thickening and hair follicle size was measured using MRM.  &amp;#xd;&amp;#xa;Results:...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2010
URL: http://precedings.nature.com/documents/4161/version/2

SLAUGHTERHOUSE RULES: HUMAN ERROR, FOOD SAFETY, AND UNIFORMITY IN MEAT PACKING
Provedor de dados: 31
Autores: Hennessy,   David A.
Meat retailers and processors express demand for a more uniform product, and technical innovations are allowing an increasingly uniform supply. Meat packers can promote uniformity through pre-slaughter sorting, or earlier through contractual procurement. Emphasizing human error and the efficacy of effort on the packing line, we develop a model whereby packers gain from expanding revenue and reducing processing costs when exogenously determined carcass uniformity increases. Line speed and occupational risk increase with uniformity. Whether optimally regulated or not, equilibrium food safety can decline with increased uniformity. Effort-saving automation also will have an adverse effect on occupational safety, and may have this effect on equilibrium food...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Contract provisions; Overload; Safety regulation; Value of information; Agricultural and Food Policy; Livestock Production/Industries.
Ano: 2003
URL: http://purl.umn.edu/18625

Slug-based epithelial-mesenchymal transition gene signature is associated with prolonged time to recurrence in glioblastoma
Provedor de dados: 17
Autores: Wei-Yi Cheng; Jessica J. Kandel; Darrell J. Yamashiro; Peter Canoll; Dimitris Anastassiou.
Background&amp;#xd;&amp;#xa;We previously identified a precise stage-associated gene expression signature of coordinately expressed genes, including the transcription factor Slug (SNAI2) and other epithelial mesenchymal transition (EMT) markers, present in samples from publicly available gene expression datasets in multiple cancer types. The expression levels of the co-expressed genes vary in a continuous and coordinate manner across the samples, ranging from absence of expression to strong co-expression of all genes. These data suggest that tumor cells may pass through an EMT like process of mesenchymal transition to varying degrees.  &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;Findings&amp;#xd;&amp;#xa;Here we show that this signature in glioblastoma multiforme...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6544/version/1

SNIPER SHOT PEGylation: TGase mediated site-specific conjugation of PEG to proteins
Provedor de dados: 17
Autores: Abhijeet Satwekar.
Commercially available recombinant protein drugs often cause immune reactions in the body which reduces their efficiency. Protein drugs can be PEGylated, (attachment of polyethylene glycol) to overcome this problem. PEGylation increases bioavailability by reduced immune reactions and decreased renal clearance [1]. So far the traditional approaches for PEGylation involve harsh reaction conditions which provide a heterogeneous product (PEG attached randomly to different sites) along with the formation of several byproducts. Due to heterogeneity, the PEGylated protein drug faces challenge for FDA approval. Therefore, there is an immense need to develop an approach which could generate a homogeneous PEGylated protein drug. &amp;#xd;&amp;#xa;The...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/5671/version/1

Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao
Provedor de dados: 86
Autores: Silva,Thiago Édson Ribeiro da; Cidade,Luciana Cardoso; Alvim,Fátima Cerqueira; Cascardo,Júlio Cézar de Mattos; Costa,Marcio Gilberto Cardoso.
The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; Cacao; Gas exchange; Micropropagation; Morphogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000024

Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of cyan fluorescent proteins at YFP-Excitation
Provedor de dados: 17
Autores: Johannes Schmid; Naila Malkani.
Background&amp;#xd;&amp;#xa;The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells or to determine protein interactions. In the latter case, the quantum physical phenomenon of fluorescence resonance energy transfer (FRET) is exploited by specific microscopy techniques to visualize proximity of proteins.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;Methodology/Principal Findings&amp;#xd;&amp;#xa;When we applied a commonly used FRET microscopy technique - the increase in donor (CFP)-fluorescence after bleaching of acceptor fluorophores (YFP), we obtained good signals in live cells, but very...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/4517/version/2

Soybean Composition Database from NIR, NMR and GC-MS Analyses
Provedor de dados: 17
Autores: I. C. Baianu.
This novel Soybean Composition Database from the AFC-NMR &amp;#x26; NIR Spectroscopy Facility of the College of ACES at the University of Illinois at Urbana includes more than 12,000 NIR measurements on soybeans from the International Soybean Germplasm Collection, such as those received from Peking at the National Soybean Collection.&amp;#xd;&amp;#xa;Excel files (.xls) of our novel spectroscopic data are currently available for all 80,000 + NIR and FT-NMR measurements; such data are made available from an ultra-fast and secure supercomputer server utilizing the current version of the Scientific-Linux OS-based software. &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;A detailed account is also presented of our high-resolution nuclear magnetic resonance (HR-NMR) and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6201/version/1

Soybean Composition Database from NIR, NMR and GC-MS Analyses
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Jun Guo; Doina M. Costescu; V. I. Prisecaru.
This novel Soybean Composition Database from the AFC-NMR &amp;#x26; NIR Spectroscopy Facility of the College of ACES at the University of Illinois at Urbana includes more than 12,000 NIR measurements on soybeans from the International Soybean Germplasm Collection, such as those received from Peking at the National Soybean Collection.&amp;#xd;&amp;#xa;Excel files (.xls) of our novel spectroscopic data are currently available for all 80,000 + NIR and FT-NMR measurements; such data are made available from an ultra-fast and secure supercomputer server utilizing the current version of the Scientific-Linux OS-based software. &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;A detailed account is also presented of our high-resolution nuclear magnetic resonance (HR-NMR) and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6201/version/2

Soybean Composition Database from NIR, NMR and GC-MS Analyses- (v.3)
Provedor de dados: 17
Autores: I. C. Baianu; Tiefeng You; Jun Guo; Doina M. Costescu; V. I. Prisecaru.
This novel Soybean Composition Database from the AFC-NMR &amp;#x26; NIR Spectroscopy Facility of the College of ACES at the University of Illinois at Urbana includes more than 12,000 NIR measurements on soybeans from the International Soybean Germplasm Collection, such as those received from Peking at the National Soybean Collection.&amp;#xd;&amp;#xa;Excel files (.xls) of our novel spectroscopic data are currently available for all 80,000 + NIR and FT-NMR measurements; such data are made available from an ultra-fast and secure supercomputer server utilizing the current version of the Scientific-Linux OS-based software. &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;A detailed account is also presented of our high-resolution nuclear magnetic resonance (HR-NMR) and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6201/version/3

Spiral Tissue Microarrays as Next Evolutionary Step in the High-density Tissue Microarray Technology
Provedor de dados: 17
Autores: Junya Fukuoka; Matthias Hofer; Takashi Hori; Tomonori Tanaka; Sayaka Tominaga; Ryo Ohsawa; Takeshi Uemura; Lucian Chirieac.
Tissue microarray (TMA) is a well-established technique that connects basic research with clinical applications that allow the validation of many pathobiologic events from gene expression dysregulation to genomic aberrations. However, conventional TMAs have several limitations such as limited representation of tissue heterogeneity, destruction of donor tissue blocks due to coring and usage of particular specimens that have limited evaluable material (tissue from thin specimens or needle biopsies). We have developed a novel method, which we termed &amp;#x22;Spiral TMA&amp;#x22; that generates TMAs that allow for improved representation of the donor tissue while keeping the architectural details of the donor block intact. This technology is ideal for...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4831/version/1

Sponges in Peru and their potential as a source of antibacterial compounds
Provedor de dados: 50
Autores: Cóndor-Luján,Báslavi; Francia-Quiroz,Juan C.
ABSTRACT In the last decades, sponges have emerged as the largest source of active compounds of animal origin for pharmaceutical purposes. In Peru information on these animals is incipient and scattered, which limits taking the right measures for their adequate management, exploitation, and conservation. The aim of this study is to provide an update on the scope and perspectives of sponge research in Peru, with an emphasis on species richness and their antibacterial potential. Likewise, research initiatives carried out in other South American Pacific countries are reviewed. Research efforts in Peru have allowed the description of 46 species; however, the sponge richness is underestimated. On the other hand, in other latitudes, active principles with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial activity; Bacteria; Biotechnology; Marine biodiversity; Porifera.
Ano: 2022
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612022000200107

SSR - Identification from EST
Provedor de dados: 17
Autores: Aikkal Riju.
Tutorial of Identification of SSR from EST resources using online Tools
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3908/version/1

Standardising bioresource acknowledgement in papers
Provedor de dados: 17
Autores: Dr Robert Hewitt.
This presentation describes the speakers experience of gathering performance indicator data, for biobank assessment.  Problems encountered in collecting data on the number of publications resulting from supported studies are described.  These include difficulty in obtaining a complete record of all the resulting publications and also frequent misnaming of the biobank in the acknowledgment section of papers.  The presentation also includes a discussion of approaches to providing credit to all the contributors in the biobanking effort.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5607/version/1

Statistical Tests for Detecting Differential RNA-Transcript Expression from Read Counts
Provedor de dados: 17
Autores: Oliver Stegle; Philipp Drewe; Regina Bohnert; Karsten Borgwardt; Gunnar R&amp;#xe4;tsch.
As a fruit of the current revolution in sequencing technology, transcriptomes can now be analyzed at an unprecedented level of detail. These advances have been exploited for detecting differential expressed genes across biological samples and for quantifying the abundances of various RNA transcripts within one gene. However, explicit strategies for detecting the hidden differential abundances of RNA transcripts in biological samples have not been defined. In this work, we present two novel statistical tests to address this issue: a &amp;#x27;gene structure sensitive&amp;#x27; Poisson test for detecting differential expression when the transcript structure of the gene is known, and a kernel-based test called Maximum Mean Discrepancy when it is unknown. We...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4437/version/1

Strategic approaches to informing the public about biotechnology in Latin America
Provedor de dados: 69
Autores: Traynor,Patricia L; Adonis,Marta; Gil,Lionel.
Abstract &lt;a href="http://fbpe/img/ejb/v10n2/a01/reprint.html"&gt;Reprint (PDF)&lt;/a&gt; The benefits of today's biotechnology products are not evident to consumers. The public will accept biotechnology only when individuals decide for themselves that biotec products will contribute to their personal well-being. To make such a decision, people will need greater awareness and understanding of how biotechnology will affect the environment, human health, local and national economies, and the well-being of society. A low level of awareness and understanding about biotechnology is characteristic of Latin America and the Caribbean countries, as elsewhere, efforts to remedy poor public perception often seem inadequate and do not reflect a well-designed...
Tipo: Journal article
Palavras-chave: Biotechnology; GMOs; Public perception.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200001

_Streptomyces_ sp. as predators of bacteria
Provedor de dados: 17
Autores: Charushila A. Kumbhar; Milind G. Watve.
Background: The ecological role of actinomycetes and their secondary metabolites is not yet completely understood. &amp;#xd;&amp;#xa;Hypothesis: Actinomycetes and Streptomyces sp. in particular, are non-obligate predators of bacteria in soil. &amp;#xd;&amp;#xa;Evidence: Ability to grow on live bacterial cells as a sole source of nutrients. Prey cell lysis accompanying growth. Circumstantial evidence for the involvement of antimicrobials along with enzymes.&amp;#xd;&amp;#xa;Implications: This finding may open up a new source of novel secondary metabolites from the genus.
Tipo: Presentation
Palavras-chave: Biotechnology; Ecology; Microbiology.
Ano: 2007
URL: http://precedings.nature.com/documents/1263/version/2

_Streptomyces_ sp. as predators of bacteria
Provedor de dados: 17
Autores: Charushila A. Kumbhar; Milind G. Watve.
Background: The ecological role of actinomycetes and their secondary metabolites is not yet completely understood. &amp;#xd;&amp;#xa;Hypothesis: Actinomycetes and Streptomyces sp. in particular, are non-obligate predators of bacteria in soil. &amp;#xd;&amp;#xa;Evidence: Ability to grow on live bacterial cells as a sole source of nutrients. Prey cell lysis accompanying growth. Circumstantial evidence for the involvement of antimicrobials along with enzymes.&amp;#xd;&amp;#xa;Implications: This finding may open up a new source of novel secondary metabolites from the genus.
Tipo: Presentation
Palavras-chave: Biotechnology; Ecology; Microbiology.
Ano: 2007
URL: http://precedings.nature.com/documents/1263/version/1

Strong violation of the competitive exclusion principle
Provedor de dados: 17
Autores: Lev V. Kalmykov; Vyacheslav L. Kalmykov.
Bacteria and plants are able to form population waves as a result of their consumer behaviour and propagation. A soliton-like interpenetration of colliding population waves was assumed but not proved earlier. Here we show how and why colliding population waves of trophically identical but fitness different species can interpenetrate through each other without delay. We have hypothesized and revealed here that the last mechanism provides a stable coexistence of two, three and four species, competing for the same limiting resource in the small homogeneous habitat under constant conditions and without any fitness trade-offs. We have explained the mystery of biodiversity mechanistically because (i) our models are bottom-up mechanistic, (ii) the revealed...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Microbiology; Bioinformatics; Plant Biology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/6667/version/1

Structural analysis of outer membrane beta-stranded porins using B-factor
Provedor de dados: 17
Autores: Abhishek Kumar; S Krishnaswamy.
Computational and statistical analysis has formed a large component of the biophysical efforts put forth to understand protein structure and function, due to the diversity and complexity of their structure. Outer membrane proteins form a diverse and complex set of proteins. Of these, porins that allow passage of molecules across the membrane interface have been analyzed here from a biophysical and structural perspective. The objective of this study is to analyze the structural organization of porins using atomic temperature factor as a parameter. Generally atomic temperature factors of molecules from crystal structures indicate the degree of mobility or disorder seen in the crystal structure. As good crystal structures have lesser possibilities of errors...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2803/version/1

Structural Analysis of Outer Membrane Beta-stranded Porins using Temperature Factor
Provedor de dados: 17
Autores: Abhishek Kumar.
Computational and statistical analysis has formed a large component of the biophysical efforts put forth to understand protein structure and function, due to the diversity and complexity of their structure. Outer membrane proteins form a diverse and complex set of proteins. Of these, porins which allow passage of molecules across the membrane interface have been analyzed here from a biophysical and structural perspective. The objective of this study is to analyze the structural organization of porins using atomic temperature factor as a parameter. Generally atomic temperature factors of molecules from crystal structures indicate the degree of mobility or disorder seen in the crystal structure. As good crystal structures have fewer possibilities of errors...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7016/version/1

Structural and Dynamic Studies of Amino Acids, Proteins and Nucleic Acids By Chemical-Hyperspectral Imaging, FT-IR/NIR, Vibro-Rotational Spectroscopy, Plasma, Microwave and Neutron Spectroscopy
Provedor de dados: 17
Autores: I. C. Baianu.
A detailed review of structural and dynamic studies of amino acids, proteins and nucleic acids by spectroscopic techniques: microwaves,  near infrared, UV, X-rays and neutrons, including also EXAFS, chemical-hyperspectral imaging and fluorescence correlation spectroscopy and FLIM, FRAP imaging with references, instrumentation diagrams and  application examples for several related areas.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7079/version/1

Structural and functional glycosphingolipidomics by glycoblotting with aminooxy-functionalized gold nanoparticle
Provedor de dados: 17
Autores: Noriko Nagahori; Midori Abe; Shin-Ichiro Nishimura.
Glycosphingolipids (GSLs) synthesized in Golgi apparatus by sequential transfer of sugar residues to a ceramide lipid anchor are ubiquitously distributing on vertebrate plasma membranes. Standardized method allowing for high throughput structural profiling and functional characterization of living cell surface GSLs is of growing importance because they function as crucial signal transduction molecules in various processes of dynamic cellular recognitions. However, methods are not available for amplification of GSLs, while the genomic scale PCR amplification permits large-scale mammalian proteomic analysis.&amp;#x3000;Here we communicate such an approach to a novel &amp;#x22;omics&amp;#x22;, namely glycosphingolipidomics based on the glycoblotting method....
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2008
URL: http://precedings.nature.com/documents/2034/version/1

Structural and functional validation of Microsystin synthetases
Provedor de dados: 17
Autores: Swapnil G. Sanmukh; Waman N. Paunikar.
The structure and function prediction for the Microsystin synthetases from Microsystis aerogenosa (LNSAMB) were carried out for verifying the authenticity of the sequenced genes. The genes for Microsystin synthetases (mcyA, mcyB, mcyD and mcyE,), were predicted by the application of computational methods and Bioinformatics web tools. The probable function prediction for the proteins was done by using Bioinformatics web tools like CDD-BLAST, INTERPROSCAN, PFAM and COGs by searching protein databases for the presence of conserved domains. While tertiary structures were constructed using PS2 Server- Protein Structure Prediction server. This study revealed structural and functional validation of sequenced genes.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6835/version/1

Structure and function prediction of human homologue hABH5 of _E. coli_ ALKB5 using in silico approach
Provedor de dados: 17
Autores: Mr. Shankaracharya; Saibal Das; Ambarish Sharan Vidyarthi.
Newly discovered human homologues of ALKB protein have shown the activity of DNA damaging drugs, used for cancer therapy. Little is known about the structure and function of hABH5, one of the members of this superfamily. Therefore, in the present study we intend to predict its structure and function using various bioinformatics tools. Modeling was done with modeler 9v7 to predict the 3D structure of the hABH5 protein. 3-D model of hABH5, ALKBH5.B99990005.pdb was predicted and evaluated. Validation results showed 96.8% residues in favor and an additional allowed region of the Ramachandran plot. Ligand binding residues prediction showed four ligand clusters, having 25 ligands in cluster 1. Importantly, conserved pattern of Pro158-X-Asp160-Xn-His266 in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5597/version/2

Structure and function prediction of human homologue hABH5 of _E. coli_ ALKB5 using in silico approach
Provedor de dados: 17
Autores: shankaracharya shankaracharya; Saibal Das; Ambarish Sharan Vidyarthi.
Newly discovered human homologues of ALKB protein have shown the activity of DNA damaging drugs, used for cancer therapy. Little is known about the structure and function of hABH5, one of the members of this superfamily. Therefore, in the present study we intend to predict its structure and function using various bioinformatics tools. Modeling was done with modeler 9v7 to predict the 3D structure of the hABH5 protein. 3-D model of hABH5, ALKBH5.B99990005.pdb was predicted and evaluated. Validation results showed 96.8% residues in favor and an additional allowed region of the Ramachandran plot. Ligand binding residues prediction showed four ligand clusters, having 25 ligands in cluster 1. Importantly, conserved pattern of Pro158-X-Asp160-Xn-His266 in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5597/version/1

Structure-based design of novel pyrazoles as aurora kinase A inhibitors.
Provedor de dados: 17
Autores: Mohane Coumar; Paritosh Shukla; Ajay Kumar Dixit; Jiun-Shyang Leou; John T. A. Hsu; Su-Ying Wu; Hsing-Pang Hsieh.
Inhibition of Aurora kinase, a member of serine/threonine kinase involved in the regulation of cell division is emerging as a new molecular targeted cancer treatment option. Three isoforms of Aurora kinase, A, B and C are known. Both Aurora A and B are over expressed in many human cancers and are linked to chromosome instability, oncogenic transformation, tumour progression and development of chemoresistance. Inhibitors of Aurora kinase, regardless of their kinase specificity spectrum have shown to promote cancer cell death by induction of apoptosis and mitotic catastrophe. Based on the current success of Aurora kinase inhibitors in the development of kinase-based cancer therapy, we have initiated a structure-based virtual screening for the identification...
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Chemistry.
Ano: 2009
URL: http://precedings.nature.com/documents/2769/version/1

Student Response to Transgenic Meat:  An analysis of a Fort Valley State University Survey
Provedor de dados: 31
Autores: Ibrahim,   Mohammed; Brewer,   Alex; Johnson,   Filton; Whitehead,   Jackie.
The study examines the factors the factors that influence Fort Valley State University students’ willing to purchase transgenic meat. Results show that respondents who said they read labels when shopping were more likely to purchase transgenic meat. Results also suggest that respondents who trust scientists to tell them the truth about transgenic meat were more likely to purchase transgenic meat.  Respondents who identified themselves as sophomores, however, were less likely to consume transgenic meat.
Tipo: Conference Paper or Presentation
Palavras-chave: Transgenic meat; Willingness to accept; Biotechnology; Genetically modified organisms; Agribusiness; Agricultural and Food Policy; Consumer/Household Economics.
Ano: 2010
URL: http://purl.umn.edu/56766

Study of Fish Weight Loss in Solar Dryer Across Different Agro-Ecological Zones of Nigeria
Provedor de dados: 17
Autores: Julius Olokor; Emeka Ugoala; Adenike Ibitoye.
Drying sessions were conducted across Nigeria to study the interaction between fish weight loss and the meteorological parameters. Correlation analyses using weight loss values show that latitude is strongly related (r =+0.96) to weight loss of fish inside the dryer while altitude had a weak relationship (r = +0.24) with weight loss. The results show that New Bussa recorded more weight loss of fish than Jos, despite their uniform latitude. Weight loss records in Jos, was however better than those of Warri and Ibadan.
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth &amp; Environment.
Ano: 2009
URL: http://precedings.nature.com/documents/3018/version/1

Substrate entering and product leaving trajectories predict an engulfing dynamic for the major conformational change of the &amp;#x3b2;-lactam acylase
Provedor de dados: 17
Autores: Xi Huang; Jun Yin; Wei-Hong Jiang; Zixin Deng; Guo-Ping Zhao.
It is still a major challenge to acquire insight into the conformational changes between the ground state and the transition state of an enzyme, although conformational fluctuation within interconverting conformers has been widely investigated (1-4). Here, we utilize different enzymatic reactions in b-lactam acylase to figure out the substrate/product trajectories in the enzyme, thereby probing the overall conformational changes in transition state. First, an auto-proteolytic intermediate of cephalosporin acylase (EC 3.5.1.11) with partial spacer segment was identified. As a final proteolytic step, the deletion of this spacer segment was revealed to be a first-order reaction, suggesting an intramolecular Ntn mechanism for the auto-proteolysis. Accordingly,...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2514/version/1

Subtyping of Dengue Viruses using Return Time Distribution based Appproach
Provedor de dados: 17
Autores: Pandurang Kolekar; Mohan Kale; Urmila Kulkarni-Kale.
Dengue virus (DENV) is the causative agent of Dengue Hemorrhagic Fever and Dengue Shock Syndrome, and continues to represent a major public health hazard. DENVs are antigenically classified in four serotypes and each serotype is further divided into respective genotypes. The association between DENV subtypes and the kind &amp;#x26; severity of disease caused by them is known. Experimental and computational approaches for subtyping are routinely used for the purpose of diagnosis and treatment of DENV, in addition to the study of phylodynamics. All virus-specific molecular subtyping tools make use of sequence alignments at backend. But as the volume of molecular data increases, alignment-dependent methods become computationally intensive. Hence, the need for...
Tipo: Poster
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Immunology; Microbiology; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5590/version/1

Successful use of axonal transport for drug delivery by synthetic molecular vehicles
Provedor de dados: 17
Autores: Aaron G. Filler; Garth Whiteside; Mark Bacon; Martyn Frederickson; Franklyn A. Howe; Miri K. Rabinowitz; Alan J. Sokoloff; Terrence Deacon; Chris Abell; Raj Munglani; John R. Griffiths; B. Anthony Bell; Andrew Lever.
We report the use of axonal transport to achieve intraneural drug delivery. We constructed a novel tripartite complex of an axonal transport facilitator conjugated to a linker molecule bearing up to a hundred reversibly attached drug molecules. The complex efficiently enters nerve terminals after intramuscular or intradermal administration and travels within axonal processes to neuron cell bodies. The tripartite agent provided 100-fold amplification of saturable neural uptake events, delivering multiple drug molecules per complex. _In vivo_, analgesic drug delivery to systemic and to non-targeted neural tissues was greatly reduced compared to existing routes of administration, thus exemplifying the possibility of specific nerve root targeting and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2164/version/1

Synchronized Mammalian Cells in Culture: A Comparison of Normal and KB Cancer Cell Growth
Provedor de dados: 17
Autores: I. C. Baianu.
Experimentation and tests with cells in culture and cell synchronization or synchrony are important in order to be able to make both reliable and high-sensitivity measurements. This is also very important for mechanistic studies of cell division control, oncogenesis and tumor growth. A comparison is made in this article between the initially synchronized cell growth of rabbit and KB cells in synchronized cultures.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7128/version/1

Synthetic Biology: opportunities for Chilean bioindustry and education
Provedor de dados: 48
Autores: Federici,Fernán; Rudge,Timothy J; Pollak,Bernardo; Haseloff,Jim; Gutiérrez,Rodrigo A.
In an age of pressing challenges for sustainable production of energy and food, the new field of Synthetic Biology has emerged as a promising approach to engineer biological systems. Synthetic Biology is formulating the design principles to engineer affordable, scalable, predictable and robust functions in biological systems. In addition to efficient transfer of evolved traits from one organism to another, Synthetic Biology offers a new and radical approach to bottom-up engineering of sensors, actuators, dynamical controllers and the biological chassis they are embedded in. Because it abstracts much of the mechanistic details underlying biological component behavior, Synthetic Biology methods and resources can be readily used by interdisciplinary teams to...
Tipo: Journal article
Palavras-chave: Synthetic biology; Bioengineering; Education; Engineering; Biotechnology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400010

Systems Biology Graphical Notation:  Process Description language Level 1
Provedor de dados: 17
Autores: Stuart L. Moodie; Nicolas  Le Novere; Emek Demir; Huaiyu  Mi; Falk  Schreiber.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialized notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields. With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/3721/version/3

Systems Biology Graphical Notation:  Process Description language Level 1
Provedor de dados: 17
Autores: Stuart L. Moodie; Nicolas  Le Novere; Anatoly  Sorokin; Huaiyu  Mi; Falk  Schreiber.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialised notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields. With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3721/version/1

Systems Biology Graphical Notation:  Process Description language Level 1
Provedor de dados: 17
Autores: Stuart L. Moodie; Nicolas  Le Novere; Emek Demir; Huaiyu Mi; Alice Villeger.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialized notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields. With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/3721/version/4

Systems Biology Graphical Notation:  Process Description language Level 1
Provedor de dados: 17
Autores: Stuart L. Moodie; Nicolas  Le Novere; Emek Demir; Huaiyu  Mi; Falk  Schreiber.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialised notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields. With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/3721/version/2

Systems Biology Graphical Notation: Process Diagram Level 1
Provedor de dados: 17
Autores: Nicolas Le Novere; Stuart Moodie; Anatoly Sorokin; Michael Hucka; Falk Schreiber; Emek Demir; Huaiyu Mi; Yukiko Matsuoka; Katja Wegner; Hiroaki Kitano.
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace. Similarly, specialised notations such as the Feynmann notation or the process flow diagrams did a lot for the adoption of concepts in their own fields. With the advent of Systems Biology, and more recently of Synthetic Biology, the need for precise and unambiguous descriptions of biochemical interactions has become more pressing. While some ideas have been advanced over the last decade, with a few detailed proposals, no actual community standard has emerged. The Systems Biology Graphical Notation (SBGN) is a graphical...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2320/version/1

Systems Biology on Bio-Linux
Provedor de dados: 17
Autores: Mesude Bicak.
This presentation introduces the Bio-Linux Operating System which is a popular Computational Biology workstation with 500+ bioinformatics software. It then provides an overview of popular modelling approaches in systems biology, covering top-down, bottom-up and hybrid modelling. Finally it highlights the challenges being faced by systems biology modellers, agent-based modellers in particular, regarding standardisation efforts.
Tipo: Presentation
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Microbiology; Molecular Cell Biology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6348/version/1

Systems Pharmacology
Provedor de dados: 17
Autores: Ravi Iyengar.
The slides are from a presentation given by Professor Ravi Iyengar from Mount Sinai School of Medicine at the Drug Forum Meeting #9 that took place in Washington, DC on February 20-21, 2008. The slides describe two projects: one that was published last year, and the other unpublished. These projects used network analysis to explore the relationships between FDA approved drugs and a human protein-protein interaction network.
Tipo: Presentation
Palavras-chave: Biotechnology; Chemistry; Pharmacology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1749/version/1

Tales From the Code Front:  Translating Modularity
Provedor de dados: 17
Autores: Lucian P. Smith.
In translating modular Antimony models to and from CellML, two differences appeared:  CellML&amp;#x27;s &amp;#x27;black box&amp;#x27; approach vs. Antimony&amp;#x27;s &amp;#x27;model composition&amp;#x27; approach, and CellML&amp;#x27;s math-based encoding vs. Antimony&amp;#x27;s object and rule-based encoding.  The latter turned out to have a much larger impact on the translation than anticipated.
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics; Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5132/version/1

TAPCells, the Chilean dendritic cell vaccine against melanoma and prostate cancer
Provedor de dados: 48
Autores: Salazar-Onfray,Flavio; Pereda,Cristián; Reyes,Diego; López,Mercedes N.
Here we summarize 10 years of effort in the development of a biomedical innovation with global projections. This innovation consists of a novel method for the production of therapeutic dendritic-like cells called Tumor Antigen Presenting Cells (TAPCells®). TAPCells-based immunotherapy was tested in more than 120 stage III and IV melanoma patients and 20 castration-resistant prostate cancer patients in a series of phase I and I/II clinical trials. TAPCells vaccines induced T cell-mediated memory immune responses that correlated with increased survival in melanoma patients and prolonged prostate-specific antigen doubling time in prostate cancer patients. Importantly, more than 60% of tested patients showed a Delayed Type Hypersensitivity (DTH) reaction...
Tipo: Journal article
Palavras-chave: Dendritic Cells; Cancer Immunotherapy; Melanoma Vaccine; Technology Transfer; Biotechnology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400014

Targeted delivery of anti-inflammatory therapy to rheumatoid tissue by fusion proteins containing an IL-4-linked synovial targeting peptide
Provedor de dados: 17
Autores: Sarah E. Wythe; Danielle DiCara; Taher E. I. Taher; Ciara M. Finucane; Rita Jones; Y. K. Stella Man; Michele Bombardieri; Ahuva Nissim; Stephen J. Mather; Yuti Chernajovsky; Costantino Pitzalis.
We provide first-time evidence that the synovial endothelium-targeting peptide (SyETP) CKSTHDRLC successfully delivers conjugated IL-4 to human rheumatoid synovium transplanted into SCID mice. SyETP, previously isolated by in vivo phage display and shown to preferentially localize to synovial xenografts, was linked by recombinant technology to hIL-4 via an MMP-cleavable sequence. Both IL-4 and the MMP-cleavable sequence were shown to be functional. IL-4-SyETP augmented production of IL-1ra by synoviocytes stimulated with IL-1[beta] in a dose-dependent manner. In vivo imaging confirmed increased retention of SyETP-linked-IL-4 in synovial grafts which was enhanced by increasing number of copies (one to three) in the constructs. Strikingly, SyETP delivered...
Tipo: Manuscript
Palavras-chave: Biotechnology; Immunology.
Ano: 2011
URL: http://precedings.nature.com/documents/6399/version/1

TATA binding protein binds to single stranded DNA: A new model for eukaryotic transcription
Provedor de dados: 17
Autores: Rostem J. Irani.
Although the players mediating the initiation of eukaryotic transcription appear to be known, the mechanism is not. In this paper we report findings on the interaction between the TATA box binding protein (TBP) and DNA, using a novel optical technology, that point to a potential plausible physical mechanism, and at the same time tie together a number of otherwise disparate observations. TBP plays a central and ubiquitous role in initiating eukaryotic transcription, binding to promoters with and without the canonical 5&amp;#x2019; TATA(A/T)A(A/T)(G/A) 3&amp;#x2019; motif1. It also tends to bind to double-stranded DNA promiscuously, and though affinity is lower, the number of binding opportunities is greater. TBP-DNA complexes have been characterized...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Microbiology; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4139/version/1

Technical Comments on &amp;#x201c;Greater Transportation Energy and GHG Offsets from BioelectricityThan Ethanol&amp;#x201d;
Provedor de dados: 17
Autores: Y-H Percival Zhang; Joe Rollin.
Campbell et al. [Report in Science 2009 (May 22),  324, 1055] analyzed two scenarios where bioenergy crops were used for transportation &amp;#x2013; (1) cellulosic ethanol production by biorefineries for internal combustion engines and (2) bioelectricity generation by power stations for electric motors. Their study suggests the latter approach offers more efficient energy utilization than the former.  We argue that the evaluation of a transportation system must be based on complicated metrics rather than only one or two factors. We suggest another out-of-the-box solution biomass carbohydrate-to-hydrogen-to-electricity as a key component of the future transportation sector.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Earth &amp; Environment.
Ano: 2009
URL: http://precedings.nature.com/documents/3725/version/1

Technology Adoption and Off-Farm Household Income: The Case of Herbicide-Tolerant Soybeans
Provedor de dados: 31
Autores: Fernandez-Cornejo,   Jorge; Hendricks,   Chad; Mishra,   Ashok K.
We model the interaction of off-farm work and adoption of agricultural technologies and the impact of adopting these technologies on farm household income from on farm and off-farm sources after controlling for such interaction, and estimate the model for the case of adoption of herbicide-tolerant (HT) soybeans using a nationwide survey of soybean farms for 2000.  We find that adoption of HT soybeans is positively and significantly related to off-farm household income for U.S. soybean farmers, after controlling for other factors.  In addition, while on-farm household income is not significantly related to adoption, total household income increases significantly with adoption.
Tipo: Journal Article
Palavras-chave: Agricultural household model; Biotechnology; Herbicide tolerant soybeans; Off-farm income; Technology adoption; O33; Q12.
Ano: 2005
URL: http://purl.umn.edu/43487

Técnicas inovadoras de melhoramento de precisão.
Provedor de dados: 14
Autores: NEPOMUCENO, A. L.
bitstream/item/194672/1/p-8-a-11-Anuario-Abrasem-2018-Final.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Agricultura de Precisão; Biotecnologia; Inovação; Precision agriculture; Biotechnology; Innovation adoption.
Ano: 2018
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1107315

Temperature factor analysis of outer membrane &amp;#x3b2;-stranded porin crystal structures suggests the pore is not uniformly rigid
Provedor de dados: 17
Autores: S. Krishnaswamy; Abhishek Kumar.
Outer membrane beta-stranded porins form a diverse and complex set of proteins which allow passage of molecules across the membrane interface have been analyzed here from a biophysical and structural perspective using atomic temperature factors or B-factors. Generally atomic temperature factors of molecules from crystal structures indicate the degree of mobility or disorder seen in the crystal structure. Structures of six porins (four 16 stranded beta barrel porins and two 8 stranded beta barrel porins) were taken from the PDB for the analysis based on resolution (better than 3.0 &amp;#xc5;) and R-factor (&amp;#x3c; 0.23). The residue distribution and mobility distribution was found to be characteristic of each of the porins. The mobility and residue...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2495/version/1

Tendril, adhesive disc and super adhesive effect of climbing plant
Provedor de dados: 17
Autores: Wenli Deng.
Understanding the super adhesion mechanism of biological system is of great scientific interest and a prerequisite for bioinspired design of adhesive systems. To investigate the versatile climbing plant requires the development of new methods. Here I present strategies to fully study the super adhesive effect of climbing plant _Parthenocissus tricuspidata_ by the first time measuring the mass and the attached area of single adhesive disc, by further determining the microscopic structure and the adhesive strength of single adhesive disc and by finally elucidating the adhesion mechanism in cellular and molecular level using the classical theories and the proposed new hypothesis and model. I have measured that a single mature adhesive disc has an average mass...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics; Plant Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/1513/version/1

Tendril, adhesive disc and super adhesive effect of climbing plant
Provedor de dados: 17
Autores: Wenli Deng.
Understanding the super adhesion mechanism of biological system is of great scientific interest and a prerequisite for bioinspired design of adhesive systems. To investigate the versatile climbing plant requires the development of new methods. Here I present strategies to fully study the super adhesive effect of climbing plant _Parthenocissus tricuspidata_ by the first time measuring the mass and the attached area of single adhesive disc, by further determining the microscopic structure and the adhesive strength of single adhesive disc and by finally elucidating the adhesion mechanism in cellular and molecular level using the classical theories and the proposed new hypothesis and model. I have measured that a single mature adhesive disc has an average mass...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1513/version/2

The &amp;#x201c;Minimum Information about an ENvironmental Sequence&amp;#x201d; (MIENS) specification
Provedor de dados: 17
Autores: Pelin  Yilmaz; Renzo Kottmann; Dawn Field; Rob Knight; James R. Cole; Linda Amaral-Zettler; Jack A. Gilbert; Ilene Karsch-Mizrachi; Anjanette  Johnston; Guy Cochrane; Robert  Vaughan; Christopher Hunter; Joonhong Park; Norman Morrison; Phillipe Rocca-Serra; Peter Sterk; Mani Arumugam; Laura Baumgartner; Bruce W. Birren; Martin J. Blaser; Vivien Bonazzi; Peer Bork; Pier Luigi Buttigieg; Patrick Chain; Elizabeth K. Costello; Heather Huot-Creasy; Peter Dawyndt; Todd DeSantis; Noah Fierer; Jed Fuhrman; Rachel E. Gallery; Richard A. Gibbs; Michelle Gwinn Giglio; Inigo San Gil; Elizabeth M. Glass; Antonio Gonzalez; Jeffrey I. Gordon; Robert Guralnick; Wolfgang Hankeln; Sarah Highlander; Philip Hugenholtz; Janet Jansson; Jerry Kennedy; Dan Knights; Omry Koren; Justin Kuczynski; Nikos Kyrpides; Robert Larsen; Christian L. Lauber; Teresa Legg; Ruth E. Ley; Catherine  A. Lozupone; Wolfgang Ludwig; Donna Lyons; Eamonn  Maguire; Barbara A. Meth&amp;#xe9;; Folker Meyer; Sara Nakielny; Karen E. Nelson; Diana Nemergut; Josh D. Neufeld; Norman R. Pace; Giriprakash Palanisamy; J&amp;#xf6;rg Peplies; Jane Peterson; Joseph Petrosino; Lita Proctor; Jeroen Raes; Sujeevan Ratnasingham; Jacques Ravel; David A. Relman; Susanna Assunta-Sansone; Lynn Schriml; Erica Sodergren; Aym&amp;#xe9; Spor; Jesse Stombaugh; James M. Tiedje; Doyle V. Ward; George M. Weinstock; Doug Wendel; Owen White; Andreas Wilke; Jennifer Wortmann; Frank Oliver Gl&amp;#xf6;ckner.
We present the Genomic Standards Consortium&amp;#x2019;s (GSC) &amp;#x201c;Minimum Information about an ENvironmental Sequence&amp;#x201d; (MIENS) standard for describing marker genes. Adoption of MIENS will enhance our ability to analyze natural genetic diversity across the Tree of Life as it is currently being documented by massive DNA sequencing efforts from myriad ecosystems in our ever-changing biosphere.
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Microbiology; Bioinformatics; Earth &amp; Environment.
Ano: 2010
URL: http://precedings.nature.com/documents/5252/version/1

The &amp;#x201c;Minimum Information about an ENvironmental Sequence&amp;#x201d; (MIENS) specification
Provedor de dados: 17
Autores: Pelin  Yilmaz; Renzo Kottmann; Dawn Field; Rob Knight; James R. Cole; Linda Amaral-Zettler; Jack A. Gilbert; Ilene Karsch-Mizrachi; Anjanette  Johnston; Guy Cochrane; Robert  Vaughan; Christopher Hunter; Joonhong Park; Norman Morrison; Phillipe Rocca-Serra; Peter Sterk; Mani Arumugam; Laura Baumgartner; Bruce W. Birren; Martin J. Blaser; Vivien Bonazzi; Peer Bork; Pier Luigi Buttigieg; Patrick Chain; Elizabeth K. Costello; Heather Huot-Creasy; Peter Dawyndt; Todd DeSantis; Noah Fierer; Jed Fuhrman; Rachel E. Gallery; Richard A. Gibbs; Michelle Gwinn Giglio; Inigo San Gil; Elizabeth M. Glass; Antonio Gonzalez; Jeffrey I. Gordon; Robert Guralnick; Wolfgang Hankeln; Sarah Highlander; Philip Hugenholtz; Janet Jansson; Jerry Kennedy; Dan Knights; Omry Koren; Justin Kuczynski; Nikos Kyrpides; Robert Larsen; Christian L. Lauber; Teresa Legg; Ruth E. Ley; Catherine  A. Lozupone; Wolfgang Ludwig; Donna Lyons; Eamonn  Maguire; Barbara A. Meth&amp;#xe9;; Folker Meyer; Sara Nakielny; Karen E. Nelson; Diana Nemergut; Josh D. Neufeld; Norman R. Pace; Giriprakash Palanisamy; J&amp;#xf6;rg Peplies; Jane Peterson; Joseph Petrosino; Lita Proctor; Jeroen Raes; Sujeevan Ratnasingham; Jacques Ravel; David A. Relman; Susanna Assunta-Sansone; Lynn Schriml; Erica Sodergren; Aym&amp;#xe9; Spor; Jesse Stombaugh; James M. Tiedje; Doyle V. Ward; George M. Weinstock; Doug Wendel; Owen White; Andreas Wilke; Jennifer Wortmann; Frank Oliver Gl&amp;#xf6;ckner.
We present the Genomic Standards Consortium&amp;#x2019;s (GSC) &amp;#x201c;Minimum Information about an ENvironmental Sequence&amp;#x201d; (MIENS) standard for describing marker genes. Adoption of MIENS will enhance our ability to analyze natural genetic diversity across the Tree of Life as it is currently being documented by massive DNA sequencing efforts from myriad ecosystems in our ever-changing biosphere.
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Genetics &amp; Genomics; Microbiology; Bioinformatics; Earth &amp; Environment; Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5252/version/2

The &amp;#x27;consciousness&amp;#x27; of the living state: Extending the Baars global broadcast model across physiological subsystems
Provedor de dados: 17
Autores: Rodrick Wallace.
For the broad spectrum of cognitive biological phenomena having &amp;#x27;dual&amp;#x27; information sources, isolation from crosstalk between them requires more metabolic free energy than permitting correlation. This allows an evolutionary exaptation leading to dynamic global broadcasts at multiple scales, similar to the well-studied exaptation of noise to trigger stochastic resonance amplification in physiological systems. Entropy gradient models adapted from nonequilibrium thermodynamics lead to an index theorem in which analytic solutions of empirical equations describe different possible topological modes. Not only is the living state characterized by cognition at every scale and level of organization, but by multiple, shifting, tunable, cooperative...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Ecology; Immunology; Molecular Cell Biology; Neuroscience; Pharmacology; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6898/version/2

The anti-angiogenic herbal composition Ob-X inhibits adipose tissue growth in obese mice
Provedor de dados: 17
Autores: Min-Young Kim; Jong Cheon Hahm; Byung-Young Park; Hee Suk Lee; Eun kyu Park; Michung Yoon; Jinmi Lee; Yeonhee Hong; Seolwha Choi; Hyunghee Lee.
Objective: The growth and development of adipose tissue are thought to be associated with angiogenesis and extracellular matrix remodeling. Since the composition of the herbal extract called Ob-X has been shown to have both anti-angiogenic and matrix metalloproteinase (MMP)-inhibiting activities, we hypothesized that growth of adipose tissue can be regulated by Ob-X. Materials and Methods: The effects of Ob-X on angiogenesis and extracellular matrix remodeling were measured using in vitro and ex vivo assays. The effects of Ob-X on adipose tissue growth were investigated with nutritionally obese mice. Results: Ob-X inhibited angiogenesis in a dose-dependent manner in the human umbilical vein endothelial cell (HUVEC) tube formation assay in vitro and the rat...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2970/version/1

The Asian Maize Biotechnology Network (AMBIONET): A Model for Strengthening National Agricultural Research Systems
Provedor de dados: 31
Autores: Pray,   Carl E.
This report reviews the impacts of the Asian Maize Biotechnology Network (AMBIONET), organized by the International Maize and Wheat Improvement Center (CIMMYT) with funding from the Asian Development Bank to strengthen the capacity of public maize research institutions in China, India, Indonesia, Philippines, Thailand, and Vietnam to produce high-yielding, disease resistant, stress tolerant maize cultivars. It was found that, during its lifetime (1998-2005), AMBIONET clearly benefited researchers and institutions in participating countries, as well as CIMMYT. In addition, there was good progress toward developing improved cultivars. Asian farmers are just beginning to gain from the work, but their future benefits will likely pay for AMBIONET’s relatively...
Tipo: Report
Palavras-chave: Zea mays; Plant breeding; Biotechnology; Breeding methods; Research methods; Disease resistance; Yield increases; Research institutions; China; India; Indonesia; Philippines; Thailand; Viet Nam; Asia; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies; F30; F01.
Ano: 2006
URL: http://purl.umn.edu/56103

The biotechnological perspective of Beta-Glucosidases
Provedor de dados: 17
Autores: Israr  Khan; Muhammad waheed Akhtar.
Biotechnology is the art and science of making and technology to process biological materials for the welfare of human being. This discussion includes the beta glucosidase classification, its structure, application, and recent research interests in relation to its significance and comparison with eukaryotic Beta glucosidases including plant, animal and yeast as well. The study also comprise of the various stress factors and modifications thereby the organism to cope it. The focus of the study is on the various kinetics principles and their full potential exploitation to increase the activity of the enzyme. As such making the enzyme a potent vehicle for various industrial, pharmaceutical and research purposes. It also include the various glycosylhydrolases...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4945/version/1

The Biotechnology Sector: "Bounds" to Market Structure
Provedor de dados: 31
Autores: Sheldon,   Ian M.
This paper examines whether it makes sense to consider Sutton's "bounds" approach as a candidate theory for explaining the recent evolution of market structure in the biotechnology sector, and to speculate whether market structure will change if the industry begins to introduce second-generation GM products that are of more direct benefit to consumers.  A key result is that the market structure is bounded in the presence of endogenous sunk costs, implying care should be taken when inferring any correlation between R&amp;D expenditure and seller concentration in the biotechnology sector.
Tipo: Conference Paper or Presentation
Palavras-chave: Biotechnology; Market structure; Research and Development/Tech Change/Emerging Technologies; L1; L11.
Ano: 2008
URL: http://purl.umn.edu/6078

The BRIF (Bioresource Research Impact Factor) as a tool for improving bioresource sharing in biomedical research
Provedor de dados: 17
Autores: Laurence Mabile; Raymond Dalgleish; Gudmundur  A. Thorisson; Myl&amp;#xe8;ne Desch&amp;#xea;nes; Robert Hewitt; Jane Carpenter; Elena Bravo; Pierre-Antoine Gourraud; Paul Hofman; Jennifer Harris; Maria Angeles Munoz-Fernandes; Anne Cambon-Thomsen.
The central aim of the BRIF (Bioresource Research Impact Factor) initiative is to construct a quantitative parameter to evaluate bioresources, modeled to some degree on the Journal Impact Factor (JIF), and to provide guidance and methodology for optimizing recognition of bioresources, their use and their sharing at international level. To implement this concept an international working group has been set up. Specific tasks have been assigned to several sub-groups: BRIF digital identifier schemes; BRIF parameters, measures and indicators; journal guidelines for resource citing and referencing; policies for bioresource access and sharing. These are discussed in the present manuscript.
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Pharmacology; Bioinformatics; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/6568/version/1

The BRIF: Success Factors and Crucial Considerations for Ensuring Impact.
Provedor de dados: 17
Autores: Lisa  B. Miranda.
This presentation focused on the potential of the BRIF to capture data that demonstrates the initial and long term impact of bioresources and those who direct/operate them for support/participation in biomedical and clinical research and their effects on clinical care outcomes. The focus of this talk is also to share ideas and provide different ways to look at the BRIF. The BRIF can be used to measure impact but may also be utilized to ensure and accelerate impact. Implementing the BRIF would potentially lead to significant positive downstream effects. Many challenges exist for the biobanking area ahead of this. One central issue that is delaying/impeding impact is the ability to create economically viable and sustainable resources.
Tipo: Presentation
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Pharmacology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5877/version/1

The Case For and Against Import Embargoes on Products of Biotechnology
Provedor de dados: 31
Autores: Gaisford,   James D.; Chui-Ha,   Carol Lau.
This article examines alternative trade policy responses available to an importing country with concerns over innovations in biotechnology. Regardless of the policy response, the importing country may be worse off after a new genetically modified food (GMF) is introduced. While an import embargo may be preferable to allowing free access to unlabelled GMF imports, permitting labelled imports is typically superior to an embargo. Thus, import embargoes on products of biotechnology should not be generally allowed. The paper provides surprising support for the existing WTO provisions on Technical Barriers to Trade, but suggests significant potential problems with the recently negotiated Biosafety Protocol.
Tipo: Journal Article
Palavras-chave: Asymmetric information; Biotechnology; Genetically modified foods; Import embargo; Labelling; International Relations/Trade.
Ano: 2000
URL: http://purl.umn.edu/23832

The Cognitive Atlas: Employing Interaction Design Processes to Facilitate Collaborative Ontology Creation
Provedor de dados: 17
Autores: Eric Miller; Christian Seppa; Aniket Kittur; Fred W. Sabb; Russell A. Poldrack.
The Cognitive Atlas is a collaborative knowledge-building project that aims to develop an ontology that characterizes the current conceptual framework among researchers in cognitive science and neuroscience. The project objectives from the beginning focused on usability, simplicity, and utility for end users. Support for Semantic Web technologies was also a priority in order to support interoperability with other neuroscience projects and knowledge bases. Current off-the-shelf semantic web or semantic wiki technologies, however, do not often lend themselves to simple user interaction designs for non-technical researchers and practitioners; the abstract nature and complexity of these systems acts as point of friction for user interaction, inhibiting...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4532/version/1

The Differences Between Products Of Gene Expression In Male, Female And Hermaphrodite Cucumber Floral Buds (Cucumis Sativus L.)
Provedor de dados: 17
Autores: Magdalena Pawelkowicz; Ewa Siedlecka; Zbigniew Przybecki.
Cucumber is a species in which sex expression has been extensively studied. Sexual differentiation is controlled by genotypic and environmental factors. The main genes responsible for sex determination have been described but the mechanism of their action remains unexplained. In this study we attempted to find cDNA clones which can be connected with sex differentiation and flower development  in cucumber. &amp;#xd;&amp;#xa;Two pairs of nearly isogenic lines: GY-3 (gynoecious &amp;#x2013; FFMMGG) and HGY-3 (hermaphrodite - FFmmGG), B10 (monoecious - ffMMGG) and 2gg (gynoecious - FFMMgg) were used to search for differences in gene expression in young (1 &amp;#x2013; 2mm) cucumber floral buds.&amp;#xd;&amp;#xa;In order to obtain differentially expressed cDNA...
Tipo: Poster
Palavras-chave: Biotechnology; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5605/version/1

The Economic Impact of Genetically Engineered Crops
Provedor de dados: 31
Autores: Zilberman,   David; Sexton,   Steven E.; Marra,   Michele C.; Fernandez-Cornejo,   Jorge.
Outstanding Choices Article Award, 2011
Tipo: Journal Article
Palavras-chave: Biotechnology; GMO; Adoption; Innovation; Productivity; Crop Production/Industries; Q160; Q240; Q420.
Ano: 2010
URL: http://purl.umn.edu/94769

The Economic Value of Patents, Licenses, and Plant Variety Protection
Provedor de dados: 31
Autores: Rausser,   Gordon C.; Small,   Arthur A.
While biotechnology creates new opportunities for agriculture, developments are impeded by confusion in the system for awarding intellectual property rights (IPRs) over agro-biotechnological innovations.  An intelligent redesign IPR system requires attention to how the definition of rights interacts with the market environment, generating incentives to create value.  A distinction is drawn between cost-reducing innovations that increase the efficiency of producing homogeneous outputs and value-adding innovations that create entirely new types of differentiated outputs.  A reform is proposed that would require the mandatory sublicensing of genes and certain core enabling technologies for creating genetically altered organisms.
Tipo: Working or Discussion Paper
Palavras-chave: Agriculture; Biotechnology; Intellectual Property; Market structure; Research and Development/Tech Change/Emerging Technologies.
Ano: 1996
URL: http://purl.umn.edu/25023

THE ECONOMICS OF BIOSAFETY: IMPLICATIONS FOR BIOTECHNOLOGY IN DEVELOPING COUNTRIES
Provedor de dados: 31
Autores: Maredia,   Mywish K.
There is a growing body of literature on the safe use of biotechnology and the need for an international biosafety protocol and national regulations to facilitate the safe development and transfer of biotechnology.  Most of these studies, however, address the issue of biosafety from a scientific, legal, environmental and organizational perspective.  The purpose of this paper is to add to this discussion by providing an economic perspective on regulating products of agricultural biotechnology, with special emphasis on implications for developing countries who are under increasing pressure to put a biosafety framework in place. The paper provides a brief discussion on the economic rationale for biosafety regulations, explains the economic benefits and costs...
Tipo: Working or Discussion Paper
Palavras-chave: Biosafety; Economic aspects; Developing countries; Agriculture; Biotechnology; Research; Research and Development/Tech Change/Emerging Technologies.
Ano: 1998
URL: http://purl.umn.edu/11768

The Economics of Biotechnology (Gmos) and the Need for A Regional Policy: The Case for COMESA Countries
Provedor de dados: 31
Autores: Minde,   Isaac J.; Kizito,   Mazvimavi.
Many countries in the world have adopted genetically modified organisms as products that can have great beneficial impact on agriculture, industry and trade. However, to date for the whole of Africa, only South Africa has commercialized genetically modified organisms (GMOs). Realizing the high transactions costs—particularly in trade that may underlie different countries having varying policy stances on biotechnology, COMESA (COMESA—Common Market for Eastern and Southern Africa, is a regional grouping of 20 countries. In the context of this study, Tanzania is also included although it is not a COMESA member. This is because it belongs to the Association for Strengthening Agricultural Research in Eastern and Central Africa (ASARECA) of which nine countries...
Tipo: Conference Paper or Presentation
Palavras-chave: Biosafety; Biotechnology; Africa; Policy; Agricultural and Food Policy; Consumer/Household Economics; Demand and Price Analysis; Environmental Economics and Policy; Food Consumption/Nutrition/Food Safety; Food Security and Poverty; International Relations/Trade; Marketing; Productivity Analysis; Research and Development/Tech Change/Emerging Technologies.
Ano: 2008
URL: http://purl.umn.edu/52150

THE ECONOMICS OF INTELLECTUAL PROPERTY RIGHTS UNDER IMPERFECT ENFORCEMENT: DEVELOPING COUNTRIES, BIOTECHNOLOGY AND THE TRIPS AGREEMENT
Provedor de dados: 31
Autores: Giannakas,   Konstantinos.
This paper develops a model of heterogeneous producers to examine the economic causes of IPR infringement and its consequences for the welfare of the interest groups and the pricing and adoption of a new technology (i.e., a genetically modified seed) in the context of a small open developing economy. Enforcement of IPRs, and pricing and adoption of the new technology are modeled as a sequential game between the government that enforces the IPRs, a foreign innovating firm that prices the new technology, and the developing country’s producers who make the production and cheating decisions. Analytical results show that complete deterrence of IPR infringement is not always economically optimal. IPR infringement affects the welfare of the interest groups and...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Enforcement; Infringement; Intellectual property rights; TRIPs agreement; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/16063

The Economics of Testing for Biotech Grain: Application to StarLink Corn
Provedor de dados: 31
Autores: Johnson,   D. Demcey; Lin,   William W.
StarLink corn, a variety not approved for human use, disrupted the marketing system in 2000 because of inadvertent commingling. This paper provides an overview of the economics of testing grain for biotech content. What are the risks facing buyers and sellers, and how are these influenced by testing protocols? How do market premiums and discounts, testing costs, and prior beliefs affect the incentives to test? A conceptual model is developed in which sellers choose whether to pre-test grain prior to shipment. Through simulation analysis, we illustrate the impact of market premiums and other variables on testing incentives and buyer risk.
Tipo: Journal Article
Palavras-chave: Biotechnology; Grain marketing; Quality risk; StarLink; Testing; Crop Production/Industries.
Ano: 2005
URL: http://purl.umn.edu/31220

The effects of anti-sense interleukin-5 gene transferred by recombinant adeno-associated virus in allergic rats
Provedor de dados: 17
Autores: Daxiong Zeng; Yong Cao; Qingfeng Song; Chao Cao; Xiansheng Liu; Yongjian Xu; Weining Xiong.
The accumulation and infiltration of eosinophils in airways is one of the most important characteristics of asthma, and is mediated partly by secretion of IL-5 from Th2 lymphocytes. It is well known that interleukin-5 (IL-5) played an important role in the regulation of eosinophils. In this study, an anti-sense IL-5 gene transferred by recombinant adeno-associated virus (rAAV-ASIL-5) was prepared to transfect allergic rats. It was found that the expression of IL-5 protein in plasma and BALF were inhibited significantly. The rAAV-ASIL-5-mediated suppression of total cell counts in peripheral blood and BALF were also observed. Moreover, rAAV-ASIL-5 remarkably reduced the eosinophil counts in peripheral blood and BALF, as well as the expression of ECP protein...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/2036/version/1

The Effects of Biotechnology Policy on Trade and Growth
Provedor de dados: 31
Autores: Oehmke,   James F.; Maredia,   Mywish K.; Weatherspoon,   Dave D.
Despite the clear influence of European Union biotechnology restrictions on trade patterns, very little work has been done to model these influences or their long-run effects. This paper presents an economic trade theory model of biotechnology, biotechnology research and development (R&amp;D), and biotechnology regulation. The model analyzes the impact of European Union consumer preferences on production and consumption of biotechnology products, the level and national origin of biotechnology R&amp;D, trade patterns, and growth. The results indicate that developing countries may become the major producers of biotechnology agricultural products if they can establish the necessary legal infrastructure; and that European biotechnology regulations effectively...
Tipo: Journal Article
Palavras-chave: Biotechnology; Economic growth; International trade; Regulation; Research and development; Research and Development/Tech Change/Emerging Technologies.
Ano: 2001
URL: http://purl.umn.edu/23872

THE EFFECTS OF INFORMATION ON CONSUMER DEMAND FOR BIOTECH FOODS:  EVIDENCE FROM EXPERIMENTAL AUCTIONS
Provedor de dados: 31
Autores: Tegene,   Abebayehu; Huffman,   Wallace E.; Rousu,   Matthew C.; Shogren,   Jason F.
Consumers' willingness to pay for food products decreases when the food label indicates that a food product is produced with the aid of modern biotechnology. This bulletin presents empirical evidence on consumers' willingness to pay for biotech foods based on the presence or absence of labels advising that the food was prepared with the aid of biotechnology. The authors designed and conducted an experimental auction to elicit consumers' willingness to pay for "genetically modified" (GM)-labeled and standard-labeled foods under different information regimes. The evidence gathered for vegetable oil, tortilla chips, and potatoes shows that labels matter. In particular, under all information treatments, consumers discounted food items labeled "GM" by an...
Tipo: Report
Palavras-chave: Biotech; Bioengineering; Biotechnology; Food labels; Auctions; Experimental economics; Random nth price; Willingness to pay; Demand and Price Analysis; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003
URL: http://purl.umn.edu/33577

The Effects of Vertical Organization on the Pricing of Differentiated Products
Provedor de dados: 31
Autores: Shi,   Guanming; Chavas,   Jean-Paul.
We investigate differentiated product pricing and the effects of vertical organization under imperfect competition. We rely on vertical measures of concentration (termed VHHI) to study how the exercise of market power varies with substitution/complementarity relationships among products and vertical structures. This approach is applied to U.S. soybean seed pricing under vertical integration versus licensing. We find evidence that vertical organization affects seed prices, with an impact ranging from 1.87% to 13.6% of the mean price. These effects vary by institutional setup.We also find that complementarity can mitigate price enhancements associated with market concentration.
Tipo: Article
Palavras-chave: Biotechnology; Imperfect competition; Pricing; Seed; Soybean; Vertical structures; Demand and Price Analysis; Industrial Organization.
Ano: 2011
URL: http://purl.umn.edu/119157

The Emergence of Insect Resistance in Bt-Corn: Implication of Resistance Management Information Under Uncertainty
Provedor de dados: 31
Autores: Linacre,   Nicholas A.; Thompson,   Colin J.
The successful management of transgenic technology is likely to depend on the economic behavioral response of farmers to the regulated use of transgenic crops. A well-studied example is the widespread use of Bt-corn, in the United States, and elsewhere, to control the European Corn Borer, a major corn pest. The extensive use of Bt-corn has led to concerns about the emergence of insect resistance. The United States Environment Protection Agency addressed this potential problem by developing an insect resistance management strategy, based, in part, on complex mathematical models using detailed biological assumptions about the population genetics and life history of the European Corn Borer. However, seed companies and others have sometimes used simpler...
Tipo: Working or Discussion Paper
Palavras-chave: Insect resistance; Monte Carlo; Bt-corn; Logistic growth; Environmental risk; Biotechnology; Environmental protection; Crop Production/Industries.
Ano: 2005
URL: http://purl.umn.edu/59239

The Entrepreneur's Choice of Location: Evidence from the Life Sciences
Provedor de dados: 31
Autores: Kolympiris,   Christos; Klein,   Peter G.; Kalaitzandonakes,   Nicholas G.
Why do biotech firms cluster? New and established firms in biotech clusters are said to capitalize on knowledge spillovers, labor-market pooling, and other externalities. Some have even argued that such spillovers are so strong that the cluster itself, rather than the individual, is the “locus of entrepreneurship.” Such arguments, however, do not resolve the mechanism by which clusters might contribute to the establishment of new firms. This paper proposes a conceptual framework for analyzing the locational choices of entrepreneurial firms in the life-sciences industry. Building on both the cluster literature and the literature on entrepreneurship, we develop hypotheses about how cluster characteristics, the entrepreneur’s personal characteristics, and...
Tipo: Conference Paper or Presentation
Palavras-chave: Entrepreneurship; Biotechnology; Clusters; Knowledge spillovers; Agglomeration economies; Industrial Organization; L26; L65; O18; O32.
Ano: 2007
URL: http://purl.umn.edu/9761

The Entropic Landscape of proteins revealing protein folding mechanism
Provedor de dados: 17
Autores: Kentaro Onizuka.
It has long since been a mystery why most proteins fold within a flash of time into particular structures out of astronomically large numbers of possible conformations. Even more confusing is that protein folding in vivo is played out in rich solution containing various organic and non-organic, big and small molecules and ions which would potentially bind the protein molecules and prevent them folding. A possible answer to these mysteries might be, &amp;#x22;Nature have favoured such proteins that quickly fold in rich solution through natural selection&amp;#x22;. Then what mechanism of folding has been favoured? Here I show how to decipher protein sequences to reveal the folding mechanism. The entropic landscape of a protein sequence tells which region of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2719/version/1

The Hepatocellular Hypoxia Criteria:2&amp;#x2019;Nitroimidazole Effect on Hepatocyte Carbohydrate Metabolizing Enzymes
Provedor de dados: 17
Autores: Rakesh Sharma.
Aim:  to understand the 2&amp;#x2019;-nitroimidazole induced hypoxia and liver cell interaction, we proposed a &amp;#x201c;Hapatocellular Hypoxia Criteria&amp;#x201d;. Hypothesis: The nitroimidazole induced metabolic energy loss and oxygen depletion (hypoxia) in liver cell mitochondria causes the phagocytosis.  Based on it, ten control subjects with 2&amp;#x2019;-nitroimidazole therapy were studied for their carbohydrate metabolizing enzymes in serum and hepatocellular enzymes in liver biopsy tissues.  Materials and Methods: Proven ten control subjects were studied for hypoxia by enzyme assays.  The 2&amp;#x2019;nitroimidazole treated paired ten subjects were studied for hypoxia using enzyme assays and hepatocellular cytomorphology by electron microscopy....
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2738/version/1

The History, Development and Impact of Computed Imaging in Neurological Diagnosis and Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities, forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/5

The History, Development and Impact of Computed Imaging in Neurological Diagnosis and Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities, forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/3

The History, Development and Impact of Computed Imaging in Neurological Diagnosis and Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities, forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/1

The History, Development and Impact of Computed Imaging in Neurological Diagnosis and Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities, forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/2

The History, Development and Impact of Computed Imaging in Neurological Diagnosis and Neurosurgery: CT, MRI, and DTI
Provedor de dados: 17
Autores: Aaron G. Filler.
A steady series of advances in physics, mathematics, computers and clinical imaging science have progressively transformed diagnosis and treatment of neurological and neurosurgical disorders in the 115 years between the discovery of the X-ray and the advent of high resolution diffusion based functional MRI. The story of the progress in human terms, with its battles for priorities, forgotten advances, competing claims, public battles for Nobel Prizes, and patent priority litigations bring alive the human drama of this remarkable collective achievement in computed medical imaging.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Neuroscience.
Ano: 2009
URL: http://precedings.nature.com/documents/3267/version/4

The Human Virome in Children and its Relationship to Febrile Illness
Provedor de dados: 17
Autores: Gregory A. Storch; George M. Weinstock; Erica  J. Sodergren; Kristine M. Wylie; Max Q. Arens; Richard S. Buller; David M. Jaffe; Jared T. Muenzer.
This study investigates the relationship of viruses to febrile illness in children.  Subjects are normal children 2-36 months of age with fever along with normal children of the same age without fever, plus immunocompromised children with fever along with immunocompromised children without fever.  Specimens obtained include blood, nasopharyngeal secretions, and feces.  Specimens are analyzed using a panel of virus-specific PCR assays and also by high throughput sequencing using 454 and Illumina platforms.
Tipo: Marker Paper / Data Plan
Palavras-chave: Biotechnology; Microbiology; Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5319/version/1

The impact of Bt cotton on poor households in rural India
Provedor de dados: 17
Autores: Arjunan Subramanian; Matin Qaim.
The impact of genetically modified (GM) crops on the poor in developing countries is still the subject of controversy. While previous studies have examined direct productivity effects of _Bacillus thuringiensis_ (Bt) cotton and other GM crops, little is known about wider socioeconomic outcomes. We develop a microeconomic modeling approach and use comprehensive survey data from India to analyze welfare and distribution effects in a typical village economy. Bt cotton adoption increases aggregate employment with interesting gender implications. Likewise, aggregate household incomes rise, including for poor and vulnerable farmers, suggesting that Bt cotton contributes to poverty reduction and rural development.
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology.
Ano: 2008
URL: http://precedings.nature.com/documents/1812/version/1

THE IMPACT OF REGULATION ON THE DEVELOPMENT OF NEW PRODUCTS IN THE FOOD INDUSTRY
Provedor de dados: 31
Autores: Menrad,   Klaus; Blind,   Knut.
Paper prepared for presentation at the 8th ICABR International Conference on Agricultural Biotechnology: International Trade and Domestic Production Ravello (Italy), July 8 to 11th, 2004
Tipo: Conference Paper or Presentation
Palavras-chave: Food industry; Regulation; Innovation; Biotechnology; Organic food; Functional Food; European Union; United States; Industrial Organization; L5; L66; O32.
Ano: 2004
URL: http://purl.umn.edu/91013

The Importance of Patents for Scientists
Provedor de dados: 17
Autores: Sue Scott.
Patents have become a hot topic and the subject of much media attention.  If your main source of information about patents is the newspapers, you could be forgiven for having an extremely distorted view of what patents are, what they do, and what benefits and problems are associated with them.   All scientists want to be able to improve the world in some way, whether by contributing in a fundamental way to the fund of human knowledge or by delivering new and valuable technology.  If you are a scientist making inventions, it is increasingly important that you should have an understanding of the importance of patents in the modern world, and should have sufficient understanding of the patent system to make sure that your aims for your inventions are...
Tipo: Presentation
Palavras-chave: Biotechnology; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/1974/version/1

The interplay of descriptor-based computational analysis with pharmacophore modeling builds the basis for a novel classification scheme for feruloyl esterases
Provedor de dados: 17
Autores: D.B.R.K. Gupta Udatha; Irene  Kouskoumvekaki; Lisbeth Olsson; Gianni  Panagiotou.
One of the most intriguing groups of enzymes, the feruloyl esterases (FAEs), is ubiquitous in both simple and complex organisms. FAEs have gained importance in biofuel, medicine and food industries due to their capability of acting on a large range of substrates for cleaving ester bonds and synthesizing high-added value molecules through esterification and transesterification reactions. During the past two decades extensive studies have been carried out on the production and partial characterization of FAEs from fungi, while much less is known about FAEs of bacterial or plant origin. Initial classification studies on FAEs were restricted on sequence similarity and substrate specificity on just four model substrates and considered only a handful of FAEs...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4762/version/1

The legacy of bio-molecules as a bio-fertilizer: Context of single cell fertilizer Isolation and partial characterization of an amylolytic bacterium
Provedor de dados: 17
Autores: Sribir Sen; Anwesha Banerjee; Sanghamitra Sanyal.
Repeated cultivation of crop plants is the reason for the depletion of nutrients in an agricultural land. Therefore, modern procedures of agriculture cascaded with the addition of organic and inorganic fertilizers, the use of insecticides and pesticides, the addition of proper water, etc. Various inorganic molecules are used as fertilizers. However, the use of organic manures is also in practice. They have many roles such as improving soil porosity, air holding capacity, water holding capacity, structure, texture, etc. Agricultural scientists suggest using organic molecules for many reasons. Bio-fertilizers of many kinds are used by farmers of all nations. However, these fertilizers are unable to cause tremendous effects on the growth and development of...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2012
URL: http://precedings.nature.com/documents/6774/version/1

The Mass-Action-Law Based GPS Concept for Bio-Informatics
Provedor de dados: 17
Autores: Ting-Chao Chou.
The unified theory of dose and effect, as indicated by the median-effect equation for single and multiple entities and for the first and higher order kinetic/dynamic, has been established by T.C. Chou and it is based on the physical/chemical principle of the mass-action law (_J. Theor. Biol._ 59: 253-276, 1976; _Pharmacological Rev._ 58: 621-681, 2006). Rearrangements of the median-effect equation lead to Michaelis-Menten, Hill, Scatchard, and Henderson-Hasselbalch equations. The &amp;#x201c;median&amp;#x201d; serves as the &amp;#x201c;universal reference point&amp;#x201d; and the &amp;#x201c;common link&amp;#x201d; for the relationship of all entities at all dynamic orders, and is also the &amp;#x201c;harmonic mean&amp;#x201d; of kinetic dissociation...
Tipo: Presentation
Palavras-chave: Biotechnology; Pharmacology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2064/version/1

The Mass-Action-Law Based GPS Concept for Bio-Informatics
Provedor de dados: 17
Autores: Ting-Chao Chou.
The unified theory of dose and effect, as indicated by the median-effect equation for single and multiple entities and for the first and higher order kinetic/dynamic, has been established by T.C. Chou and it is based on the physical/chemical principle of the mass-action law (_J. Theor. Biol._ 59: 253-276, 1976; _Pharmacological Rev._ 58: 621-681, 2006). Rearrangements of the median-effect equation lead to Michaelis-Menten, Hill, Scatchard, and Henderson-Hasselbalch equations. The &amp;#x201c;median&amp;#x201d; serves as the &amp;#x201c;universal reference point&amp;#x201d; and the &amp;#x201c;common link&amp;#x201d; for the relationship of all entities at all dynamic orders, and is also the &amp;#x201c;harmonic mean&amp;#x201d; of kinetic dissociation...
Tipo: Presentation
Palavras-chave: Biotechnology; Pharmacology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/2064/version/2

The Modified Pharaoh Approach: Stingless bees mummify beetle parasites alive
Provedor de dados: 17
Autores: Mark K. Greco; Dorothee Hoffmann; Anne Dollin; Michael Duncan; Robert Spooner-Hart; Peter Neumann.
Social insect colonies usually live in nests, which are often invaded by parasitic species. Workers from these colonies use different defence strategies to combat invaders. Nevertheless, some parasitic species are able to bypass primary colony defences due to their morphology and behaviour. In particular, some beetle nest invaders cannot be killed or removed by workers of social bees, thus creating the need for alternative social defence strategies to ensure colony survival. Here we show, using Diagnostic Radioentomology, that stingless bee workers _Trigona carbonaria_, immediately mummify invading destructive nest parasites _Aethina tumida_ alive, with a mixture of resin, wax and mud, thereby preventing severe damage to the colony. In sharp contrast to...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth &amp; Environment.
Ano: 2009
URL: http://precedings.nature.com/documents/2591/version/2

The Modified Pharaoh Approach: Stingless bees mummify beetle parasites alive
Provedor de dados: 17
Autores: Mark K. Greco; Peter Neumann; Dorothee Hoffmann; Anne Dollin; Michael Duncan; Robert Spooner-Hart.
Social insect colonies usually live in nests, which are often invaded by parasitic species^1^. Workers from these colonies use different defence strategies to combat invaders^1^. Nevertheless, some parasitic species are able to bypass primary colony defences due to their morphology and behaviour^1-3^. In particular, some beetle nest invaders cannot be killed or removed by workers of social bees^2-5^, thus creating the need for alternative social defence strategies to ensure colony survival. Here we show, using Diagnostic Radioentomology^6^, that stingless bee workers _Trigona carbonaria_, immediately mummify invading destructive nest parasites _Aethina tumida_ alive, with a mixture of resin, wax and mud, thereby preventing severe damage to the colony. In...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth &amp; Environment.
Ano: 2008
URL: http://precedings.nature.com/documents/2591/version/1

The Molecular In Silico Dissection of Cancer using TCLASS and its Application in the Identification of the Tissue of Origin of &amp;#xb;Cancers of Unknown Primary Site&amp;#xb;
Provedor de dados: 17
Autores: Torik Ayoubi.
Traditional cancer diagnosis relies on a combination of clinical and histo-pathological data. In many cases, however, the morphology of in particular metastatic cancer is not discriminative enough for correct diagnosis. In addition, the morphology of tumor cells do not provide information on aberrant molecular pathways that may be used to specifically target the tumor like the application of Herceptin to breast cancers with amplification in the ERBB2 gene. With the completion of the human genome project, molecular pathology has dramatically increased its arsenal of novel molecular biomarkers that can be used to determine the phenotype of tumors. Gene expression profiling using high-density microarray technology is an extremely powerful tool to...
Tipo: Poster
Palavras-chave: Biotechnology; Cancer; Genetics &amp; Genomics; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/5300/version/1

The Neonatal Microbiome and Necrotizing Enterocolitis.
Provedor de dados: 17
Autores: Phillip I. Tarr; Barbara Warner; Erica Sodergren; William Shannon; Aaron Hamvas; Vincent Magrini; George Weinstock.
Necrotizing enterocolitis (NEC) is a devastating disorder that affects approximately 10% of premature infants. Its mortality remains high (15-30%), and its cause remains unknown. About 80% of cases occur within 35 days of birth among hospitalized newborns of low birth weight. Probiotics diminish the incidence and severity of NEC, and NEC does not occur antepartum. NEC affects a readily identifiable at-risk group, has a tightly defined interval before its onset, occurs in an organ system that is intimately associated with a microbial population in flux, has a plausible association with the intestinal microbiota, and cohorts at risk have rarely been studied in large numbers, or prospectively. This disorder, therefore, provides a unique opportunity to explore...
Tipo: Marker Paper / Data Plan
Palavras-chave: Biotechnology; Developmental Biology; Genetics &amp; Genomics; Microbiology; Bioinformatics; Data Standards.
Ano: 2010
URL: http://precedings.nature.com/documents/5285/version/1

The Nuclear Imaging Uncertainty Principle. Do Nuclear Cameras Really Work?
Provedor de dados: 17
Autores: Richard Fleming; Gordon Harrington.
The introduction of the Heisenberg Uncertainty principle and Nuclear Cardiology occurred simultaneously in 1925-1927. Thirty years later the Anger gamma camera would allow for a more sophisticated radioactive isotope counting to determine the presence or absence of disease. When employed with technetium-99m isotopes, ischemic heart disease can be inferred by differences in visual appearance of cardiac images. These gestalts of imaging results have been separated from the quantitative information recorded by the cameras computer. We investigated whether current camera and computer systems are sophisticated enough to quantify differences between images to be clinically relevant. Our study demonstrated that efforts to &amp;#x22;sharpen&amp;#x22; image...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2010
URL: http://precedings.nature.com/documents/5013/version/1

The Open Practises E-Science Network (OPEN)
Provedor de dados: 17
Autores: Cameron  Neylon.
A grant proposal submitted for support to fund a research network focussed on identifying and dealing with the practical issues of enabling open practise in research. The text of the proposal was written by a large number of people and coordinated by Cameron Neylon.
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/1370/version/1

The Private Sector in Agricultural R&amp;D: Policies and Institutions to Foster its Growth in Developing Countries
Provedor de dados: 31
Autores: Naseem,   Anwar; Omamo,   Steven Were; Spielman,   David J.
New technologies are critical to enhancing agricultural productivity and reducing poverty in many developing countries. While public-sector investment in research has historically driven technological change in agriculture, recent trends suggest that the public sector’s role may not be as significant in the future. There is much optimism about the private sector’s capacity to deliver new technologies, even though current levels of private investment in research in developing countries remain low. This paper examines the determinants of private investment in agricultural research and development in developing countries, the market and institutional constraints that limit private investment growth, and the incentive mechanisms that can strengthen private...
Tipo: Working or Discussion Paper
Palavras-chave: Agricultural research and development; Private sector; Agricultural productivity; Research and development; Poverty reduction; Investments; Biotechnology; Food policy; Agricultural research; Research and Development/Tech Change/Emerging Technologies.
Ano: 2006
URL: http://purl.umn.edu/58582

The Production and Price Impact of Biotech Crops
Provedor de dados: 31
Autores: Brookes,   Graham; Yu,   Tun-Hsiang (Edward); Tokgoz,   Simla; Elobeid,   Amani E.
Biotech crops have now been grown commercially on a substantial global scale since 1996. This paper examines the production effects of the technology and impacts on cereal and oilseed markets through the use of agricultural commodity models. It analyses the impacts on global production, consumption, trade and prices in the soybean, canola and corn sectors. The analysis suggests that world prices of corn, soybeans and canola would probably be, respectively, 5.8%, 9.6% and 3.8% higher, on average, than 2007 baseline levels if this technology was no longer available to farmers. Prices of key derivatives of soybeans (meal and oil) would also be between 5% and 9% higher, with rapeseed meal and oil prices being about 4% higher than baseline levels. World prices...
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; Cereals; Crop yields; Oilseeds; Price effects; Productivity.; Crop Production/Industries; Demand and Price Analysis; Productivity Analysis.
Ano: 2010
URL: http://purl.umn.edu/56984

The Proteomics World is Flat: Dependence of Regulation upon Abundance
Provedor de dados: 17
Autores: David Good; Roman Zubarev.
The long-standing assumption that proteins divide into house-keeping and cell-specific groups was recently struck down after discovering that the vast majority of expressed proteins are shared across cell lines. Here, we tested a related hypothesis that abundant proteins are less regulated than low-abundance ones. Meta-analysis of published data revealed that protein regulation is only weakly dependent upon the protein abundance, thus providing support to cell characterization by the &amp;#x22;top&amp;#x22; proteome.
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6412/version/1

The relationship among knowledge of, attitudes toward and acceptance of genetically modified organisms (GMOs) among Slovenian teachers
Provedor de dados: 69
Autores: Sorgo,Andrej; Ambrozis-Dolinsek,Jana.
The objective of this study was to investigate knowledge about, opinions on and attitudes toward and finally readiness to accept genetically modified organisms (GMO) among Slovenian teachers. On average, they have higher levels of knowledge in classical genetics, and poor levels of knowledge about modern issues in biotechnology, and their attitudes toward GMOs are not extreme. They make decisions based on the acceptability of a particular GMO and not on GMOs in general, following two patterns: genetic modifications (GM) microorganisms and plants are more acceptable than animals, and GMOs are more acceptable if they can not be used directly for consumption and produce something recognized as useful. The relationship among knowledge of, attitudes towards and...
Tipo: Journal article
Palavras-chave: Acceptance; Attitudes; Biotechnology; Education; Genetically modified organisms; Knowledge.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400001

The Reproducibility of Lists of Differentially Expressed Genes in Microarray Studies
Provedor de dados: 17
Autores: Leming D. Shi; Wendell D. Jones; Roderick V. Jensen; Stephen C. Harris; Roger G. Perkins; Federico M. Goodsaid; Lei Guo; Lisa J. Croner; Cecilie Boysen; Hong Fang; Shashi Amur; Wenjun Bao; Catalin C. Barbacioru; Vincent Bertholet; Xiaoxi Megan Cao; Tzu-Ming Chu; Patrick J. Collins; Xiao-hui Fan; Felix W. Frueh; James C. Fuscoe; Xu Guo; Jing Han; Damir Herman; Huixiao Hong; Ernest S. Kawasaki; Quan-Zhen Li; Yuling Luo; Yunqing Ma; Nan Mei; Ron L. Peterson; Raj K. Puri; Feng Qian; Richard Shippy; Zhenqiang Su; Yongming Andrew Sun; Hongmei Sun; Brett Thorn; Yaron Turpaz; Charles Wang; Sue-Jane Wang; Janet A. Warrington; James C. Willey; Jie Wu; Qian Xie; Liang Zhang; Lu Zhang; Sheng Zhong; Russell D. Wolfinger; Weida Tong.
Reproducibility is a fundamental requirement in scientific experiments and clinical contexts.  Recent publications raise concerns about the reliability of microarray technology because of the apparent lack of agreement between lists of differentially expressed genes (DEGs).  In this study we demonstrate that (1) such discordance may stem from ranking and selecting DEGs solely by statistical significance (P) derived from widely used simple t-tests; (2) when fold change (FC) is used as the ranking criterion, the lists become much more reproducible, especially when fewer genes are selected; and (3) the instability of short DEG lists based on P cutoffs is an expected mathematical consequence of the high variability of the t-values.  We recommend the use of FC...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/306/version/2

The Reproducibility of Lists of Differentially Expressed Genes in Microarray Studies
Provedor de dados: 17
Autores: Leming Shi; Wendell D. Jones; Roderick V. Jensen; Stephen C. Harris; Roger G. Perkins; Federico M. Goodsaid; Lei Guo; Lisa J. Croner; Cecilie Boysen; Hong Fang; Shashi Amur; Wenjun Bao; Catalin C. Barbacioru; Vincent Bertholet; Xiaoxi Megan Cao; Tzu-Ming Chu; Patrick J. Collins; Xiao-hui Fan; Felix W. Frueh; James C. Fuscoe; Xu Guo; Jing Han; Damir Herman; Huixiao Hong; Ernest S. Kawasaki; Quan-Zhen Li; Yuling Luo; Yunqing Ma; Nan Mei; Ron L. Peterson; Raj K. Puri; Feng Qian; Richard Shippy; Zhenqiang Su; Yongming Andrew Sun; Hongmei Sun; Brett Thorn; Yaron Turpaz; Charles Wang; Sue-Jane Wang; Janet A. Warrington; James C. Willey; Jie Wu; Qian Xie; Liang Zhang; Lu Zhang; Sheng Zhong; James J. Chen; Russell D. Wolfinger; Weida Tong.
Reproducibility is a fundamental requirement in scientific experiments and clinical contexts.  Recent publications raise concerns about the reliability of microarray technology because of the apparent lack of agreement between lists of differentially expressed genes (DEGs).  In this study we demonstrate that (1) such discordance may stem from ranking and selecting DEGs solely by statistical significance (P) derived from widely used simple t-tests; (2) when fold change (FC) is used as the ranking criterion, the lists become much more reproducible, especially when fewer genes are selected; and (3) the instability of short DEG lists based on P cutoffs is an expected mathematical consequence of the high variability of the t-values.  We recommend the use of FC...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/306/version/1

The Risks and Benefits of Genetically Modified Crops: A Multidisciplinary Perspective
Provedor de dados: 7
Autores: Peterson, Garry D; McGill University; garry.peterson@mcgill.ca; Cunningham, Saul; CSIRO Entomology; saul.cunningham@ento.csiro.au; Deutsch, Lisa; Department of Systems Ecology, Stockholm University; lisad@system.ecology.su.se; Erickson, Jon; Rensselaer Polytechnic Institute; erickj@rpi.edu; Quinlan, Allyson; Conservation Ecology; aquinlan@resalliance.org; Tinch, Robert; School of Environmental Sciences, University of East Anglia; R.Tinch@uea.ac.uk; Troell, Max; Beijer Institute, Royal Swedish Academy of Sciences; max@system.ecology.su.se; Woodbury, Peter; Boyce Thompson Institute for Plant Research; pbw1@cornell.edu; Zens, Scot; Department of Biology, Dartmouth College; zens@dartmouth.edu.
The benefits and risks of any particular GM crop depend on the interactions of its ecological functions and natural history with the agroecosystem and ecosystems within which it is embedded. These evolutionary and ecological factors must be considered when assessing GM crops. We argue that the assessment of GM crops should be broadened to include alternative agricultural practices, ecosystem management, and agricultural policy. Such an assessment would be facilitated by a clearer understanding of the indirect costs of agriculture and the ecological services that support it. The benefits of GM crops should be compared to those of other means of agricultural intensification such as organic farming, integrated pest management, and agricultural policy reform....
Tipo: Peer-Reviewed Reports
Palavras-chave: Agriculture; Biotechnology; Genetically modified crops (GM); Interdisciplinary; Public dialogue; Regulation; Risk assessment.
Ano: 2000

THE ROLE OF SOCIOECONOMIC FACTORS AND LIFESTYLE VARIABLES IN ATTITUDE AND THE DEMAND FOR GENETICALLY MODIFIED FOODS
Provedor de dados: 31
Autores: Heiman,   Amir; Just,   David R.; Zilberman,   David.
Consumer resistance is a key barrier to the diffusion of genetically modified foods (GMFs). Several studies have shown that consumers in general have a negative attitude toward GMFs. Through analysis of a survey conducted in Israel, we find consumer attitudes toward GMFs to be context specific, differing based on the available alternatives. Consumers responded positively to genetically modified meats when given the alternative choice of meats produced with hormones or dyes.  We also address the importance of gender, education, and being religious on the consumer's attitude toward GMFs. Both education and being religious have significant effects on attitude, while gender does not.
Tipo: Journal Article
Palavras-chave: Biotechnology; Consumer attitudes; Diffusion; Genetically modified foods; Food Consumption/Nutrition/Food Safety; Research and Development/Tech Change/Emerging Technologies.
Ano: 2000
URL: http://purl.umn.edu/14713

THE SEED INDUSTRY IN U.S. AGRICULTURE: AN EXPLORATION OF DATA AND INFORMATION ON CROP SEED MARKETS, REGULATION, INDUSTRY STRUCTURE, AND RESEARCH AND DEVELOPMENT
Provedor de dados: 31
Autores: Fernandez-Cornejo,   Jorge.
The unprecedented growth in crop yields and agricultural total factor productivity over the past 70 years owes much to a series of biological innovations embodied in seeds, beginning with the development of hybrid crops in the United States in the early part of the 20th century, continuing with the adoption of high-yielding varieties during the Green Revolution of the 1960s and 1970s, and more recently, modern biotechnology. Throughout this period, the seed industry evolved, as small businesses gave way to larger enterprises that integrated plant breeding, production, conditioning, and marketing functions. The industry was further shaped by widespread mergers and acquisitions in the latter part of the century, rapid growth - in private research and...
Tipo: Report
Palavras-chave: Seed markets; Seed costs and prices; Regulation; Plant breeding; Field crops; Research and development; Industry concentration; Biotechnology; Agribusiness; Crop Production/Industries.
Ano: 2004
URL: http://purl.umn.edu/33671

The Simulation Experiment  Description Markup Language - Update
Provedor de dados: 17
Autores: Frank T. Bergmann.
This presentation gives a brief overview of the Simulation Experiment Description Markup Language L1V1 as well as an introduction to LibSedML (http://libsedml.sf.net) a library for reading and writing SED-ML documents.
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/5589/version/1

THE SOURCE OF COMPARATIVE ADVANTAGE IN THE BIOTECHNOLOGY INDUSTRY: A REAL OPTIONS APPROACH
Provedor de dados: 31
Autores: Lavoie,   Brian F.; Sheldon,   Ian M.
Sources of heterogeneity within the process of R&amp;D investment, such as international differences in the maximum per-period rate of investment and level of regulatory uncertainty, offer a plausible explanation for US comparative advantage in biotechnology. Using dynamic stochastic simulation, the results presented in this paper suggest US biotechnology firms may initiate more R&amp;D projects, innovate earlier and more rapidly, persevere longer in the face of mounting R&amp;D costs, and successfully complete more R&amp;D projects than European firms.
Tipo: Conference Paper or Presentation
Palavras-chave: Comparative advantage; Biotechnology; Real options; Industrial Organization; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/21508

The study on estimation of time since death with proton magnetic resonance spectroscopy under different temperature
Provedor de dados: 17
Autores: Tiantong Yang; Li Zhenwei; Wu Yue; Liu Liang; Zheng Na.
Estimation of the time of death (post-mortem interval, PMI) is occasionally a critical issue in adjudication of a criminal or civil action. But no objective and precise means has been derived for estimating PMI.One reason for this outcome may be the fact that most of these methods attempted to characterize PMI by a few or even just one specific parameter&amp;#x27;s variable in constant ambient temperature. It is crucial that we reduce or eliminate the effect of temperature variation in the estimation of PMI. Here we show the first results by using proton magnetic resonance spectroscopy (1H-MRS) technique to determine the order of degradation of certain substances, present in the brain tissue of a rabbit model, under different temperatures; our goal is to...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2007
URL: http://precedings.nature.com/documents/1363/version/1

The Systems Biology Markup Language (SBML) Level 2 Version 2
Provedor de dados: 17
Autores: Michael Hucka; Sarah M. Keating.
The Systems Biology Markup Language (SBML) is a machine-readable model representation language for software tools in computational systems biology. By supporting SBML as an input/output format, different tools can all operate on an identical representation of a model, removing opportunities for translation errors and assuring a common starting point for analyses and simulations. SBML is by no means a perfect format, but it has achieved widespread acceptance as a de facto standard. It is supported worldwide by over 100 software systems (both open-source and commercial). The broad acceptance of a common, open format for exchanging models between software tools is a crucial step towards wider use of quantitative modeling in biology, because it allows...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/19/version/1

The Systems Biology Markup Language (SBML): Where It&amp;#x27;s Been and Where It&amp;#x27;s Going
Provedor de dados: 17
Autores: Michael Hucka.
A cornerstone of systems biology is the use of computational modeling, by which hypotheses can be cast into a quantitative form that can be tested systematically.  The use of computational modeling by biologists promises to pave the way for more rigorous analyses of biological functions, and ultimately will lead to new and better treatments for disease.&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;A crucial enabler for more widespread use of computational modeling in biology is reaching agreement on how to represent, store, and communicate models between software tools. The Systems Biology Markup Language (SBML) project is an effort to create a machine-readable format for representing computational models in biology.  By supporting SBML as an input and output...
Tipo: Presentation
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2007
URL: http://precedings.nature.com/documents/21/version/1

The timing of evaluation of genebank accessions and the effects of biotechnology
Provedor de dados: 31
Autores: Koo,   Bonwoo; Wright,   Brian D.
The lack of ex-ante evaluation of germplasm in genebanks has been the single most prevalent and long-standing complaint of plant breeders about the management of genebanks. Advances in biotechnology offer the possibility of faster, cheaper, and more efficient evaluation methodologies. Will these new technologies favor ex-post evaluation, as some expect, or will it lead to more ex-ante evaluation? Will it also lead to earlier development of varieties with disease resistance traits in anticipation of actual infestations? Will the prospect of further advances in biotechnology favor delay of evaluation and development? This paper addresses these questions in the case of evaluation of germplasm for resistance to a disease.
Tipo: Working or Discussion Paper
Palavras-chave: Gene banks; Plant; Biotechnology; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999
URL: http://purl.umn.edu/97514

The U.S. Seed Industry: An Exploration of Statistics Highlighting the Economic Activity of the U.S. Row Crop Seed Industry
Provedor de dados: 31
Autores: Roucan-Kane,   Maud; Gray,   Allan W.
This report presents relevant statistics that highlight the economic activity of the U.S. seed industry. The focus of this report is on the four main U.S. crops: corn, soybean, wheat, and cotton. The report contains three sections. The first is related to the U.S. seed market’s size based on seed sales and expenditures. The second section examines industry investment in research and development (R&amp;D) activity in terms of both budget and human resources. The final section illustrates the impact of the seed industry in terms of intellectual property development, improved productivity, and other benefits. This report focuses on using publicly available data to examine these three areas. In addition, the report presents the results of a survey conducted...
Tipo: Working or Discussion Paper
Palavras-chave: Seed industry; Field crops; Biotechnology; Herbicide-tolerant crops; Bt crops; Corn; Soybeans; Cotton; Wheat.; Agribusiness; L11; L16; L65; O33; O34; Q16.
Ano: 2009
URL: http://purl.umn.edu/52549

The use of coral as a graft in a large cortical bone defect
Provedor de dados: 17
Autores: Hassan Z. Eskandar; Wan Zulmi; Abdul Hamid Suzina; Abd Rani Samsudin.
The use of coral as space filler in a large cortical bone defect was studied. Twelve Australian White Rabbit were divided into 8 and 12 week group. All rabbits were operated to create a 2.5cm defect in right tibia and replaced with coral; partially covered with the free autologous non-vascularized periosteum harvested from contralateral tibia. The coral was transfixed and the leg was immobilized with Plaster of Paris. At the end of 8th and 12th week, all the rabbits were assessed clinically, radiologically and sacrified for histology evaluation. Bony union achieved in 3 out of 5 rabbits at 8th week while 3 out of 4 at 12th supported by radiological studies. Histological examination showed healing at coral-cortical junction by callus formation with...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2008
URL: http://precedings.nature.com/documents/2264/version/1

Three Dimensional Quantitative Structure-Activity Relationships of Sulfonamides Binding Monoclonal Antibody by Comparative Molecular Field Analysis
Provedor de dados: 17
Autores: Zhanhui  Wang; Xingyuan Cao; Zhenpeng  Kai; Ross C. Beier; Congming Wu; Haiyang Jiang; Linli Cheng; Jianzhong Shen; Xinling Yang.
The three-dimensional quantitative structure-activity relationship (3D-QSAR) model of sulfonamide analogs binding a monoclonal antibody (MabSMR) produced against sulfamerazine, was carried out by comparative molecular field analysis (CoMFA). The affinities of MabSMR, expressed as Log10IC50, for 17 sulfonamide analogs were determined by competitive fluorescence polarization immunoassay (FPIA). Removal of two outliers from the initial set of 17 sulfonamide analogs improved the predictability of the models. The 3D-QSAR model of 15 sulfonamides resulted in q2cv values of 0.600, and r2 values of 0.995, respectively. This novel study combining FPIA with CoMFA demonstrates that multidisciplinary research can be used as a useful tool to investigate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry; Pharmacology.
Ano: 2008
URL: http://precedings.nature.com/documents/2646/version/1

Tissue culture parameters in sweet orange cultivars
Provedor de dados: 86
Autores: Mendes,Amanda Ferreira da Silva; Cidade,Luciana Cardoso; Manzoli,Gabrielle Novais; Otoni,Wagner Campos; Soares Filho,Walter dos Santos; Costa,Marcio Gilberto Cardoso.
The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; Biotechnology; Gene transfer; Morphogenesis; Regeneration.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800020

TO REACH THE POOR: RESULTS FROM THE ISNAR-IFPRI NEXT HARVEST STUDY ON GENETICALLY MODIFIED CROPS, PUBLIC RESEARCH, AND POLICY IMPLICATIONS
Provedor de dados: 31
Autores: Atanassov,   Atannas; Bahieldin,   Ahmed; Brink,   Johan; Burachik,   Moises; Cohen,   Joel I.; Dhawan,   Vibha; Ebora,   Reynaldo V.; Falck-Zepeda,   Jose Benjamin; Herrera-Estrella,   Luis; Komen,   John; Low,   Fee Chon; Omaliko,   Emeka; Odhiambo,   Benjamin; Quemada,   Hector; Peng,   Yufa; Sampaio,   Maria Jose; Sithole-Niang,   Idah; Sittenfeld,   Ana; Smale,   Melinda; Sutrisno; Valyasevi,   Ruud; Zafar,   Yusuf; Zambrano,   Patricia.
Local farming communities throughout the world face productivity constraints, environmental concerns, and diverse nutritional needs. Developing countries address these challenges in a number of ways. One way is public research that produces genetically modified (GM) crops and recognize biotechnology as a part of the solution. To reach these communities, GM crops, after receiving biosafety agreement, must be approved for evaluation under local conditions. However, gaps between approvals in the developed and developing world grow larger, as the process of advancing GM crops in developing countries becomes increasingly difficult. In several countries, only insect resistant cotton has successfully moved from small, confined experimental trials to larger, open...
Tipo: Working or Discussion Paper
Palavras-chave: Biosafety; Regulation; Biotechnology; Genetic modification; Public research; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2004
URL: http://purl.umn.edu/16065

Topological Characterization of Protein-Protein Interaction Networks in Human and Mouse
Provedor de dados: 17
Autores: Bipin Singh.
The elucidation of the cell&amp;#x27;s large-scale organization is a primary challenge for post-genomic biology, and understanding the structure and topological properties of protein-protein interaction networks offers an important starting point for such studies. We compare the protein-protein interaction network of the human and mouse, aiming to uncover the network&amp;#x27;s generic large-scale properties and the impact of the protein&amp;#x27;s function and cellular localization on the network topology. We show that both human and mouse protein-protein interaction supports a scale-free, topology with hierarchical modularity, indicating that these features represent a robust and generic property of the protein interactions network. We also find strong...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6126/version/1

Towards a genome-wide transcriptogram: the Saccharomyces cerevisiae case
Provedor de dados: 17
Autores: Jos&amp;#xe9; Luiz Rybarczyk; Mauro A. A. Castro; Rodrigo J. S. Dalmolin; Jos&amp;#xe9; C. F. Moreira; Leonardo G. Brunnet; Rita M. C. de Almeida.
A genome modular classification that associates cellular processes to modules could lead to a method to quantify the differences in gene expression levels in different cellular stages or conditions: the transcriptogram, a powerful tool for assessing cell performance, would be at hand. Here we present a computational method to order genes on a line that clusters strongly interacting genes, defining functional modules associated with gene ontology terms. The starting point is a list of genes and a matrix specifying their interactions, available at large gene interaction databases. Considering the Saccharomyces cerevisiae genome we produced a succession of plots of gene transcription levels for a fermentation process. These plots discriminate the fermentation...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3869/version/1

Towards a Grey to Green Revolution
Provedor de dados: 30
Autores: W.D.
Palavras-chave: Green revolution; Chickpeas; Biotechnology; Pigeonpeas; Watershed management; Private sector; Groundnuts; Watersheds; Tropical zones; Sorghum.
Ano: 2002
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3727

Tracing blastomere fate choices of early embryos in single cell culture
Provedor de dados: 17
Autores: Yunhan Hong; Haobin Zhao; Ni Hong; Zhendong Li; Meisheng Yi; Rong Liu; Mingyou Li; Yan Yan; Yongming Yuan; Chang Ming Li; Ruowen Ge; Jianxin Song.
Blastomeres of early vertebrate embryos undergo numerous fate choices for division, motility, pluripotency maintenance and restriction culminating in various cell lineages. Tracing blastomere fate choices at the single cell level in vitro has not been possible because of the inability to isolate and cultivate early blastomeres as single cells. Here we report the establishment of single cell culture system in the fish medaka, enabling the isolation and cultivation of individual blastomeres from 16- to 64-cell embryos for fate tracing at the single cell level in vitro. Interestingly, these blastomeres immediately upon isolation exhibit motility, lose synchronous divisions and even stop dividing in &amp;#x2265;50% cases, suggesting that the widely accepted...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Molecular Cell Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4907/version/1

Transformacao de feijoeiro com gene Lhcb1*2 por meio do metodo SAAT ("Sonication-assited-agrobacterium transformation").
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de; GOMES, D. G.; LABATE, M. T. V.; LABATE, C. A.
bitstream/CPAC-2009/24345/1/p2002_71.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Transformacao genetica; Transgenico; Transgenics.; Biotecnologia; Feijão; Gene; Melhoramento Genético Vegetal; Phaseolus Vulgaris.; Biotechnology; Genetic transformation; Beans; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567647

Transformacao de Phaseolus vulgaris Via SAAT ("Sonication-assisted agrobacterium- mediated transformation").
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de; GOMES, D. G.; LABATE, M. T. V.; LABATE, C. A.
bitstream/CPAC-2009/24346/1/p2002_72.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Transgenico; Transformacao genetica; Transgenics.; Biotecnologia; Feijão; Gene; Melhoramento Genético Vegetal; Phaseolus Vulgaris.; Genetics; Beans; Biotechnology; Genetic transformation; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567648

Transformation and Regeneration of Groundnut, and Utilization of Viral Genes to Induce Resistance to Virus Diseases
Provedor de dados: 30
Autores: D.V.R.
Palavras-chave: Genes; RNA; Transgenics; Protoplasts; Groundnuts; Transgenic plants; Biotechnology; Regeneration; Genomes; Nucleotides.
Ano: 1992
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4105

Transforming the Axiomisation of Ontologies: The Ontology Pre-Processor Language
Provedor de dados: 17
Autores: Mikel Ega&amp;#xf1;a Aranguren; Robert Stevens; Erick Antezana.
As ontologies are developed there is a common need to transform them, especially from those that are axiomatically lean to those that are axiomatically rich. Such transformations often require large numbers of axioms to be generated that affect many different parts of the ontology. This paper describes the Ontology Pre-Processor Language (OPPL), a domain-specific macro language, based in the Manchester OWL Syntax, for manipulating ontologies written in OWL. OPPL instructions can add/remove entities, and add/remove axioms (semantics or annotations) to/from entities in an OWL ontology. OPPL is suitable for applying the same change to different ontologies or at different development stages, and for keeping track of the changes made (e.g. in pipelines). It is...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/4006/version/1

Transgene effects on rhizodeposition: Evidence from molecular-chemical screening by Pyrolysis-Field Ionisation Mass Spectrometry (Py-FIMS)
Provedor de dados: 17
Autores: Andre  Schlichting; Christel  Baum; Dana  Zimmer; Peter  Leinweber.
Rhizodeposition plays an important role in the C and N cycle of ecosystems, since it essentially reflects the interaction between plant, soil and soil microorganisms. The molecular-chemical characterisation of rhizodeposition is often limited to selected compound classes (e.g., carbohydrates and amino acids). A more comprehensive analytical approach is based on the molecular-chemical &amp;#x201c;fingerprint&amp;#x201d; revealed by Pyrolysis-Field Ionisation Mass Spectrometry (Py-FIMS).&amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;The presented results give evidence of specific effects in conjunction with soil type, crop, variety, transgenes and mycorrhizal colonisation on the molecular-chemical composition of rhizodeposition. The experiments included various crops...
Tipo: Poster
Palavras-chave: Biotechnology; Chemistry; Plant Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/4440/version/1

Transgenic Trees: Implementation and Outcomes of the Plant Protection Act
Provedor de dados: 31
Autores: Sedjo,   Roger A.
The responsibility for protecting U.S. agriculture from pests and diseases is assigned by the Federal Plant Pest Act (FPPA) to the Animal and Plant Health Inspection Service (APHIS) of the Department of Agriculture. The Plant Protection Act (Title 7 U.S.C. Sections 7701 et seq.) gives Aphis statutory authority over genetically modified organisms (GMO), in effect assigning to APHIS a related responsibility of determining whether a genetically altered plant, crop, or tree is likely to pose unacceptable risks to the environment. Although APHIS has considerable experience with crop plants, it has only limited experience with trees. Yet the possible benefits of applying genetic engineering to trees are substantial and include industrial wood production and...
Tipo: Working or Discussion Paper
Palavras-chave: Forestry; Biotechnology; Transgenic; Tree plantations; Timber supply; Genes; GMOs; Industrial wood; Economics; Regulations; Costs; Benefits; Conservation; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 2004
URL: http://purl.umn.edu/10629

Transient-mediated fate determination in a transcriptional circuit of HIV
Provedor de dados: 17
Autores: Leor Weinberger; Roy D. Dar; Michael Simpson.
Steady-state behavior and bistability have been proposed as mechanisms for decision-making in gene circuits. However, transient gene expression has also been proposed to control cell fate with the decision arbitrated by the lifetime of the expression transient. Here, we report that transcriptional positive-feedback plays a critical role in determining HIV infected cell-fate by extending the duration of Tat expression transients far beyond what protein half-life modulation can achieve. To directly quantify feedback strength and its effects on the duration of Tat transcriptional pulses, we exploit the noise inherent to gene-expression and measure shifts in the autocorrelation of expression noise. The results indicate that transcriptional positive-feedback...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology.
Ano: 2007
URL: http://precedings.nature.com/documents/1198/version/1

Translational Oncogenomics and Human Cancer Interactome Networks
Provedor de dados: 17
Autores: I. C. Baianu.
An overview of translational, human oncogenomics, transcriptomics and cancer interactomic networks is presented together with basic concepts and potential, new applications to Oncology and Integrative Cancer Biology. Novel translational oncogenomics research is rapidly expanding through the application of advanced technology, research findings and computational tools/models to both pharmaceutical and clinical problems. A self-contained presentation is adopted that covers both fundamental concepts and the most recent biomedical, as well as clinical, applications. Sample analyses in recent clinical studies have shown that gene expression data can be employed to distinguish between tumor types as well as to predict outcomes. Potentially important applications...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2011
URL: http://precedings.nature.com/documents/6190/version/2

Transparent Exopolymer Particles (TEP): an overlooked factor in the process of biofilm formation in aquatic environments
Provedor de dados: 17
Autores: Tom Berman; Uta Passow.
We hypothesize that transparent exopolymer particles (TEP), present in high concentrations in most sea and freshwaters, are critical agents for biofilm initiation and development in many natural and anthropogenic aquatic environments. These gel-like particles appear in many forms; amorphous blobs, clouds, sheets, filaments or clumps ranging in size from ~2 to ~200 &amp;#xb5;m. TEP are mostly polysaccharide, negatively charged, very sticky and are frequently colonized by bacteria. TEP may be considered a &amp;#x22;planktonic&amp;#x22; subgroup of exopolymeric substances (EPS), widely studied in biofilm research. Recognition of TEP involvement in biofilm formation has important implications for a comprehensive understanding of the complexities of this...
Tipo: Manuscript
Palavras-chave: Biotechnology; Microbiology.
Ano: 2007
URL: http://precedings.nature.com/documents/1182/version/1

Trichoderma reesei derived cellulase activity in three N,N-dimethylethanolammonium akylcarboxylate ionic liquids
Provedor de dados: 17
Autores: Sierra Rayne; Giuseppe Mazza.
The activity and denaturation extent of cellulase from Trichoderma reesei (E.C. # 3.2.1.4) was investigated in three representative N,N-dimethylethanolammonium akylcarboxylate ionic liquids. Significant cellulase activity and absence of enzyme unfolding was found in all concentrations of N,N-dimethylethanolammonium acetate (DMEAA), including the pure liquid. Activities in 20% and 40%  (v/v) solutions of DMEAA were equal to citrate buffer controls. Lower enzymatic activities and denaturation were observed in solutions of the corresponding formate and octanoate ionic liquids, although cellulose hydrolysis still proceeded at a substantial rate. The results provide the first proof-of-principle that cellulose can be enzymatically hydrolyzed in the presence of...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2007
URL: http://precedings.nature.com/documents/632/version/1

Trichoscopy criteria for diagnosing female androgenic alopecia.
Provedor de dados: 17
Autores: Adriana Rakowska; Monika Slowinska; Elzbieta Kowalska-Oledzka; Malgorzata Olszewska; Lidia Rudnicka.
Differential diagnosis of chronic hair loss remains a challenge in dermatology. The aim of the study was to evaluate the value of a new scalp visualization technique, trichoscopy, in differential diagnosis of hair loss. Trichoscopy was performed in 131 females (59 with androgenic alopecia, 33 with chronic telogen effluvium, 39 healthy controls). Based study results, a standardized trichoscopy report was developed and diagnostic criteria for female androgenic alopecia were established. Major criteria: increased number of yellow dots and thin hairs, as well as decreased average hair thickness in frontal area. Minor criteria: increased frontal area to occiput ratio of single-hair units (&amp;#x3e;2:1), vellus hairs (&amp;#x3e;1.5:1) and follicles with...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/1913/version/1

Trust in Scientists and Food Manufacturers: Implications for the Public Support of Biotechnology
Provedor de dados: 31
Autores: James,   Harvey S., Jr.
An analysis of data from the U.S. Biotechnology Study, 1997S1998 reveals that few variables affect public trust in scientists, while variables representing perceived benefits, risks, trustworthiness, and competence affect trust in food manufacturers on matters of biotechnology. Both trust in scientists and trust in food manufacturers have a large and important effect on public support for biotechnology, although trust in scientists is found to be more important than trust in food manufacturers. Findings of this analysis suggest that trust in scientists is relatively generalized, while trust in food manufacturers is particularized.
Tipo: Journal Article
Palavras-chave: Biotechnology; Genetic modification; Public support of biotechnology; Public trust; Trust; Agribusiness; Food Consumption/Nutrition/Food Safety.
Ano: 2006
URL: http://purl.umn.edu/62273

Trypsin digest of cancer cells surface stimulates anti-tumor immune response better than cancer cells themselves
Provedor de dados: 17
Autores: Petr Lokhov; Balashova Elena.
Antigens expressed on the surface of cancer cells are accessible targets for both humoral and cell-mediated immune responses, and are therefore potential candidates for vaccine development. Treating surface of live human breast adenocarcinoma cells (MCF-7) with trypsin yields a digest that contains 0.7% of total cell protein. Despite this difference, the trypsin digest stimulates in cytotoxicity assays anti-tumor response which kills 10-40% more cancer cells than those stimulated with cells themselves. From these results, we concluded that trypsin digest obtained from live cancer cells contains the essential antigens to induce an immune-mediated anti-tumor effect, and therefore, is candidate for anti-tumor vaccine development.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Immunology.
Ano: 2010
URL: http://precedings.nature.com/documents/4895/version/1

Tuning and regulating the repertoire of glycan determinants
Provedor de dados: 17
Autores: Rodrick Wallace; Deborah Wallace.
We apply Tlusty&amp;#x27;s information-theoretic index theorem analysis of the genetic code to the glycome, using a cognitive paradigm by which external information sources constrain and tune the glycan code error network, in the context of available metabolic energy. The model suggests spontaneous symmetry breaking of the glycan code as a function of metabolic energy intensity, an effect that may be currently present, or embedded in evolutionary trajectory, recording large-scale ecosystem resilience shifts in energy availability such as the aerobic transition. Once focused on a subset of the glycan error code network however, the glycan production machinery must then be regulated by an elaborate cognitive process to ensure that what is produced matches...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Ecology; Molecular Cell Biology; Pharmacology; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5932/version/2

Ultrasound-targeted microbubble destruction enhances AAV mediated gene transfection: human RPE cells in vitro and the rat retina in vivo
Provedor de dados: 17
Autores: Hongli Li; Xiaozhi Zheng; Huiping Wang; Fan Li; Ying Wu; Lianfang Du.
The present study was performed to investigate the efficacy and safety of Ultrasound-targeted microbubble destruction (UTMD) mediated rAAV2-EGFP to cultured human retinal pigment epithelium (RPE) cells _in vitro_ and the rat retina _in vivo_. _In vitro_ study, cultured human RPE cells were exposed to US under different conditions with or without microbubbles. Furthermore, the effect of UTMD to rAAV2-EGFP itself and the cells were evaluated. _In vivo_ study, gene transfer was examined by injecting rAAV2-EGFP into the subretinal space of the rats with or without microbubbles and then exposed to US. We investigated EGFP expression _in vivo_ via stereomicroscopy and performed quantitative analysis by Axiovision 3.1 software. HE staining and frozen sections...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2009
URL: http://precedings.nature.com/documents/2898/version/1

Underlying Mathematics in Diversification of Human Olfactory Receptors in Different Loci
Provedor de dados: 17
Autores: Sk. Sarif  Hassan; Pabitra  Pal Choudhury; Arunava Goswami.
As per conservative estimate, approximately (51-105) Olfactory Receptors (ORs) loci are present in human genome occurring in clusters. These clusters are apparently unevenly spread as mosaics over 21 pair of human chromosomes. Olfactory Receptor (OR) gene families which are thought to have expanded for the need to provide recognition capability for huge number of pure and complex odorants. ORs form the largest known multi-gene family in the human genome. Recent studies have shown that 388 full length and 414 OR pseudo-genes are present in these OR genomic clusters. In this paper, the authors report a classification method for all human ORs based on their sequential quantitative information like presence of poly strings of nucleotides bases, long range...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2010
URL: http://precedings.nature.com/documents/5475/version/1

Understanding Genomic Evolution of Olfactory Receptors through Fractal and Mathematical Morphology
Provedor de dados: 17
Autores: Sk. Sarif Hassan; Pabitra Pal Choudhury; B. S. Dayasagar; Shantanav Chakraborty; Ranita  Guha; Arunava Goswami.
Fractals and Mathematical Morphology are immensely used to study many problems in different branches of science and technology including the domain of Biology. There are many more unrevealed facts and figures of genes and genome in Computational Biology. In this paper, our objective is to explore how the evolutionary network is associated among Human, Chimpanzee and Mouse with regards to their genomic information. We are about to explore their genomic evolution through the quantitative measures of fractals and morphology. We have considered olfactory receptors for our case study. These olfactory receptors do function in different species with subtle differences in the structures of DNA sequences. Those subtle differences can be exposed through intricate...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5674/version/1

Understanding Hydrogen-Bond Patterns in Proteins using a Novel Statistical Model
Provedor de dados: 17
Autores: Ofer Rahat; Uri Alon; Yaakov Levy; Gideon Schreiber.
Proteins are built from basic structural elements and their systematic characterization is of interest. Searching for recurring patterns in protein contact maps, we found several network motifs, patterns that occur more frequently in experimentally determined protein contact maps than in randomized contact maps with the same properties. Some of these network motifs correspond to sub-structures of alpha helices, including topologies not previously recognized in this context. Other motifs characterize beta-sheets, again some of which appear to be novel. This topological characterization of patterns serves as a tool to characterize proteins, and to reveal a high detailed differences map for comparing protein structures solved by X-ray crystallography, NMR and...
Tipo: Manuscript
Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2008
URL: http://precedings.nature.com/documents/1490/version/1

Unique features of Plasmids among different Citrobacter species
Provedor de dados: 17
Autores: Swapnil G. Sanmukh; Waman N. Paunikar.
The _Citrobacter_ plasmids are supposed to represent the host genetic association within the living bacterial cell. The plasmids impart various beneficial characteristics to the host, helping it to retain suitable characteristics for adaptation as well as evolution. The study aims at understanding the role of prophage in influencing host functional characteristics by horizontal gene transfer or as whole plasmids. The  _Citrobacter_ plasmid can be understood by analyzing many hypothetical protein sequences within its genome. Our study included 82 hypothetical proteins in 5 _Citrobacter_ plasmids genomes. The function predictions in 31 hypothetical proteins and 3-D structures were predicted for 11 protein sequences using PS2 server. The probable function...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Bioinformatics; Evolutionary Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6836/version/1

Unique for human centromeric regions of interphase chromatin homing (CENTRICH) govern dynamic features of chromatin fractal globules
Provedor de dados: 17
Autores: Gennadi Glinsky.
We report the results of the genome-wide alignment of inter- and intra-chromosomal chromatin interactions within the context of interphase chromatin binding to nuclear lamina and nucleolus. For all human chromosomes, a significant correlation exists between binding of chromosomal loci to nuclear lamina and segregation into spatially-defined distinct compartments of genome-wide chromatin interactions identified by Hi-C method. We report identification of near-centromeric intergenic regions on human chromosomes (chr2; chr10; chr17; chr1), which are highly enriched for interphase chromatin homing sites and function as attractors of long-range physical interactions (Centromeric Regions of Interphase Chromatin Homing, CENTRICH). CENTRICH are engaged in 397-1526...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6756/version/1

Universality of a mesenchymal transition signature in invasive solid cancers
Provedor de dados: 17
Autores: Dimitris Anastassiou.
In this brief communication, additional computational validation is provided consistent with the unifying hypothesis that a shared biological mechanism of mesenchymal transition, reflected by a precise gene expression signature, may be present in all types of solid cancers when they reach a particular stage of invasiveness.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/6862/version/1

Unlocking biomarker discovery: Large scale application of aptamer proteomic technology for early detection of lung cancer
Provedor de dados: 17
Autores: Rachel M. Ostroff; William L. Bigbee; Wilbur Franklin; Larry Gold; Mike Mehan; York E. Miller; Harvey I. Pass; William N. Rom; Jill M. Siegfried; Alex Stewart; Jeffrey J. Walker; Joel L. Weissfeld; Stephen Williams; Dom Zichi; Edward N. Brody.
Lung cancer is the leading cause of cancer deaths, because ~84% of cases are diagnosed at an advanced stage. Worldwide in 2008, ~1.5 million people were diagnosed and ~1.3 million died &amp;#x2013; a survival rate unchanged since 1960. However, patients diagnosed at an early stage and have surgery experience an 86% overall 5-year survival. New diagnostics are therefore needed to identify lung cancer at this stage. Here we present the first large scale clinical use of aptamers to discover blood protein biomarkers in disease with our breakthrough proteomic technology. This multi-center case-control study was conducted in archived samples from 1,326 subjects from four independent studies of non-small cell lung cancer (NSCLC) in long-term tobacco-exposed...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Molecular Cell Biology; Bioinformatics.
Ano: 2010
URL: http://precedings.nature.com/documents/4537/version/1

Use of inadequate data and methodological errors lead to a dramatic overestimation of the water footprint of Jatropha curcas
Provedor de dados: 17
Autores: Wouter  Maes; Wouter M. J. Achten; Bart Muys.
In their recent article, Gerbens-Leenes et al. (1) calculated the water footprint (WF, the amount of water required to produce 1 GJ of energy) of several bioenergy crops. One of the most remarkable findings of this study was the very high water footprint of this species, which has serious management consequences. &amp;#xd;&amp;#xa;&amp;#xd;&amp;#xa;However, these results are in apparent contrast with recent findings on this species. We present evidence that several errors were made by the authors when calculating the water footprint of jatropha, which has lead to a dramatic overestimation. These errors include weaknesses concerning the data used for the calculation of the water footprint, as well as flaws in the calculation method, as we demonstrate in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Ecology; Earth &amp; Environment; Plant Biology.
Ano: 2009
URL: http://precedings.nature.com/documents/3410/version/1

Uso de marcadores RAPD e do sistema de informacao geografica no estudo da variabilidade genetica de Stylosanthes macrocephala.
Provedor de dados: 14
Autores: BARROS, A. M.; FALEIRO, F. G.; SHIRATSUCHI, L. S.; KARIA, C. T.; LOPES, G. K. B.; ANDRADE, R. P.
bitstream/CPAC-2009/24956/1/p2004_09.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: RAPD; Stylosanthes macrocepala; Feed legumes.; Biogeografia; Biotecnologia; Marcador Genético; Marcador Molecular; Leguminosa Forrageira; Sistema de Informação Geográfica; Variação Genética.; Biotechnology; Genetic variation; Geographic information systems; Biogeography; Genetic markers; Molecular biology.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567934

USP4 is regulated by Akt phosphorylation and deubiquitylates TGF-beta type I receptor
Provedor de dados: 17
Autores: Long Zhang; Fangfang Zhou; Yvette Drabsch; Ewa  Snaar-Jagalska; Craig Mickanin; Huizhe Huang; Kelly-Ann Sheppard; Chris Lu; Peter ten Dijke.
Stability and membrane localization of Transforming growth factor-&amp;#x3b2; (TGF-&amp;#x3b2;) type I receptor (T&amp;#x3b2;RI) is essential for controlling TGF-&amp;#x3b2; signaling. T&amp;#x3b2;RI is targeted for ubiquitination-mediated degradation by Smad7/Smurf2 complex. However, it is unclear whether polyubiquitin modified T&amp;#x3b2;RI can be reversed. Here we performed a genome-wide gain of function screen and identified ubiquitin-specific protease (USP) 4 as a strong inducer of TGF-&amp;#x3b2; signaling. Putative oncogenic USP4 was found to interact with T&amp;#x3b2;RI as deubiquitinating enzyme thus maintains T&amp;#x3b2;R1 levels at the plasma membrane. Depletion of USP4 mitigates TGF-&amp;#x3b2;-induced breast cancer cell migration, epithelial...
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6804/version/1

Utilizacao de marcadores moleculares em retrocruzamentos visando a resistencia do maracujazeiro-azedo a multiplas doencas.
Provedor de dados: 14
Autores: FALEIRO, F. G.; JUNQUEIRA, N. T. V.; BELON, G.; KRALH, L. L.; ANJOS, J. de R. N. dos; PEIXOTO, J. R.; BRAGA, M. F.; REZENDE, A. M.
bitstream/CPAC-2009/24952/1/p2004_06.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Melhoramento genetico; Plant diseases.; Biotecnologia; Doença de Planta; Marcador Molecular; Marcador Genético; Maracujá; Passiflora Edulis; Retrocruzamento; Variedade Resistente.; Biotechnology; Genetic markers; Disease resistance; Passion fruits; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/566509

VALUATION OF BIOTECHNOLOGY COMPANIES: REAL OPTIONS APPROACH UNDER UNCERTAINTY
Provedor de dados: 31
Autores: Ljumovic,   Isidora; Cvijanovic,   Janko; Lazic,   Jelena.
Review article
Tipo: Article
Palavras-chave: Risk; Real options; Valuation; Biotechnology; Agribusiness; Risk and Uncertainty; D04; D81; L65.
Ano: 2012
URL: http://purl.umn.edu/123958

Variabilidade genética de acessos de mandioca morfologicamente similares à etnovariedade 'buriti' com base em marcadores moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FIALHO, J. de F.; BELLON, G.; VIEIRA, E. A.; FUKUDA, W. M. G.
bitstream/CPAC-2009/27695/1/p2006_34.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Biotecnologia; Marcador Genético; Marcador Molecular; Mandioca; Manihot Esculenta; Variação Genética.; Genetic variation.; Biotechnology; Cassava; Genetic markers.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570172

Variabilidade genética de acessos de mandioca similares às variedades 'Pioneira' e 'Japonesa' mantidos em banco de germoplasma e cultivados em núcleos rurais com base em marcadores moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FIALHO, J. de F.; BELLON, G.; VIEIRA, E. A.; FUKUDA, W. M. G.
bitstream/CPAC-2009/27697/1/p2006_36.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Biotecnologia; Mandioca; Manihot Esculenta; Marcador Genético; Marcador Molecular; Variação Genética.; Genetic variation.; Genetic markers; Biotechnology; Cassava.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570174

Variabilidade genética de acessos de maracujá-suspiro (Passiflora nitida Kunth.) com base em marcadores moleculares.
Provedor de dados: 14
Autores: JUNQUEIRA, K. P.; FALEIRO, F. G.; RAMOS, J. D.; BELLON, G.; PAULA, M. da S.; JUNQUEIRA, N. T. V.; BRAGA, M. F.
bitstream/CPAC-2009/27694/1/p2006_33.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Biotecnologia; Marcador Genético; Marcador Molecular; Maracujá; Passiflora Edulis; Variação Genética.; Genetic markers; Biotechnology; Genetic variation; Passion fruits.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570171

Variabilidade genética de cultivares de coqueiro anão e híbridos com base em marcadores moleculares RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; VARGAS RAMOS, V. H.; BELLON, G.; JUNQUEIRA, K. P.; AZEVEDO, J. A.; ARAGÃO, W. M.
bitstream/CPAC-2009/27700/1/p2006_39.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Biotecnologia; Coco; Cocos Nucifera; Marcador Molecular; Variação Genética.; Genetic markers; Coconuts; Genetic variation.; Biotechnology.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570177

Variabilidade genética de Mahanarva spectabilis (Distant, 1909) coletada em brachiaria brizantha cv. marandu no estado de Tocantins, com base em marcadores RAPD.
Provedor de dados: 14
Autores: PAULA-MORAES, S. V. de; FALEIRO, F. G.; VILELA, M. de F.; JUNQUEIRA, K. P.; BELLON, G.; CARVALHO, G. S.; AUAD, A. M.; TAKADA, S.; PONTES, R. A.; GONÇALVES NETO, S. de C.
bitstream/CPAC-2009/27699/1/p2006_38.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Pests of plants; Feed grasses.; Biotecnologia; Capim Brachiaria; Cigarrinha; Gramínea Forrageira; Marcador Genético; Marcador Molecular; Praga de Planta; Variação Genética.; Genetic variation.; Biotechnology; Genetic markers.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570176

Variabilidade genética de Mahanarva spectabilis (Distant, 19090) coletadas em diferentes locais e hospedeiros, com base em marcadores moleculares RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; PAULA-MORAES, S. V. de; JUNQUEIRA, K. P.; BELLON, G.; RAMOS, A. K. B.; VILELA, M. de F.; CARVALHO, G. S.; AUAD, A. M.; TAKADA, S.
bitstream/CPAC-2009/27698/1/p2006_37.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Pests of plants.; Biotecnologia; Cigarrinha; Marcador Genético; Marcador Molecular; Praga de Planta; Variação Genética.; Genetic variation.; Biotechnology; Genetic markers.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570175

Variabilidade genetica de plantas de Stylosanthes guianensis var. vulgaris com base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; BARCELLOS, A. de O.; ANDRADE, R. P.; KARIA, C. T.; RAMOS, A. K. B.; COELHO, M. H.; PINTO, J. F. N.
bitstream/CPAC-2009/24948/1/p2004_02.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: RAPD; Feed legumes.; Leguminosa Forrageira; Marcador Molecular; Melhoramento Genético Vegetal; Variação Genética.; Genetic variation; Genetic markers; Biotechnology; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567821

Viability of liquid medium-inoculation of Rhizobium etli in planting furrows with common bean
Provedor de dados: 86
Autores: Oliveira,Dâmiany Pádua; Soares,Bruno Lima; Martins,Fábio Aurélio Dias; Franceschini,Leandro Alex; Cardillo,Bruno Ewerton da Silveira; Rufini,Márcia; Morais,Augusto Ramalho de; Moreira,Fatima Maria de Souza; Andrade,Messias José Bastos de.
Abstract: The objective of this work was to evaluate the viability of liquid medium inoculation of Rhizobium etli in the planting furrow and to certify the efficiency of its strain UFLA 02-100 as a potential inoculant for common bean (Phaseolus vulgaris). The treatments consisted of three application rates of liquid inoculant applied in the planting furrows or in common bean seed, besides two controls without inoculation. The inoculant contained 109 cells of Rhizobium etli mL-1. Regardless of the application method, the yield obtained with the inoculation was equivalent to that of N from urea; however, the application in the furrows, at 0.6 L ha-1, is more advantageous due to the operational practicality and reduced costs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Biological N2 fixation; Biotechnology.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300394

Visual Molecular Dynamics Investigations of the Impact of Hydrophobic Nanoparticles on Prognosis  of Alzheimer&amp;#x2019;s Disease and Cancers
Provedor de dados: 17
Autores: I. C. Baianu; M Charles; V. I. Prisecaru.
The possible impact of hydrophobic lectin nanoparticles on the prognosis and progression of Alzheimer&amp;#x27;s disease (AD) and cancers was investigated by Visual Molecular Dynamics (VMD) computer modeling programs available from the Beckmann Advanced Research Institute at the University of Illinois at Urbana. Our results indicate the possibility of impeding pathological aggregation of certain proteins such as modified tau- or beta-amyloid that are currently being considered as possible causes of Alzheimer&amp;#x27;s disease. VMD programs serve as useful tools for investigation hydrophobic protein aggregation that may play a role in aging of human populations.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics &amp; Genomics; Molecular Cell Biology; Neuroscience; Pharmacology; Bioinformatics; Data Standards.
Ano: 2012
URL: http://precedings.nature.com/documents/7111/version/2

Visual Molecular Dynamics Investigations of the Impact of Hydrophobic Nanoparticles on Prognosis  of Alzheimer&amp;#x2019;s Disease and Cancers
Provedor de dados: 17
Autores: I. C. Baianu; M Charles; V. I. Prisecaru.
The possible impact of hydrophobic lectin nanoparticles on the prognosis and progression of Alzheimer&amp;#x27;s disease (AD) and cancers was investigated by Visual Molecular Dynamics (VMD) computer modeling programs available from the Beckmann Advanced Research Institute at the University of Illinois at Urbana. Our results indicate the possibility of impeding pathological aggregation of certain proteins such as modified tau- or beta-amyloid that are currently being considered as possible causes of Alzheimer&amp;#x27;s disease. VMD programs serve as useful tools for investigation hydrophobic protein aggregation that may play a role in aging of human populations.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics &amp; Genomics; Neuroscience; Pharmacology; Bioinformatics.
Ano: 2012
URL: http://precedings.nature.com/documents/7111/version/1

Visualization of the Phosphoproteomic Data from AfCS with the Google Motion Chart Gadget
Provedor de dados: 17
Autores: Huilei Xu; Avi Ma&amp;#x27;ayan.
Results from multivariate molecular biological experiments become increasingly complex. Hence, the challenge of projecting high-dimensional data onto few dimensions for effective data visualization is becoming increasingly important in Systems Biology. Effective data visualization can summarize the activity of many variables over time as well as display relationships between variables. Dynamic interactive visualization tools can provide scientists with ways of visually identifying relationship and patterns, and improve communication of results on the web and in presentations. For this, interactive systems with animation have great potential since they add dimensions to static images limited to two dimensions. Interactivity and animation is particularly...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3074/version/1

VO: Vaccine Ontology
Provedor de dados: 17
Autores: Yongqun He; Lindsay  Cowell; Alexander D. Diehl; Harry  Mobley; Bjoern Peters; Alan  Ruttenberg; Richard H. Scheuermann; Ryan  R. Brinkman; Melanie  Courtot; Chris  Mungall; Zuoshuang  Xiang; Fang Chen; Thomas Todd; Lesley  Colby; Howard  Rush; Trish  Whetzel; Mark A. Musen; Brian D. Athey; Gilbert  S. Omenn; Barry  Smith.
Vaccine research, as well as the development, testing, clinical trials, and commercial uses of vaccines involve complex processes with various biological data that include gene and protein expression, analysis of molecular and cellular interactions, study of tissue and whole body responses, and extensive epidemiological modeling. Although many data resources are available to meet different aspects of vaccine needs, it remains a challenge how we are to standardize vaccine annotation, integrate data about varied vaccine types and resources, and support advanced vaccine data analysis and inference. To address these problems, the community-based Vaccine Ontology (VO,...
Tipo: Poster
Palavras-chave: Biotechnology; Immunology; Microbiology; Bioinformatics.
Ano: 2009
URL: http://precedings.nature.com/documents/3552/version/1

We Need A Web For Data
Provedor de dados: 17
Autores: John Wilbanks.
The Web has many forums for sharing personal data, but not for scientific data and not in a way that allows the data to be accessed by &amp;#x22;machines as users.&amp;#x22; A Web of data could add tremendous value by integrating disparate disciplines or conduct data-driven queries. Doing this is very complex and requires more robust standards than currently exist. The intended user for most data is not a person; it is a software application that can manipulate the data into something useful for humans. Such software could be &amp;#x22;search engines, analytic software, visualization tools, database back ends, and more.&amp;#x22; This need creates a much different requirement for standards than those that were developed for displaying web data to people....
Tipo: Manuscript
Palavras-chave: Biotechnology; Data Standards.
Ano: 2011
URL: http://precedings.nature.com/documents/5499/version/1

Weight matrix based identification of terpene synthases conserved motifs in Arabidopsis thaliana proteome
Provedor de dados: 17
Autores: Suchita  Srivastava; Suaib  Luqman; Feroz  Khan.
Terpenes comprise the most diverse collection of natural products. Out of more than 30,000 individual terpenoids identified, at least half are synthesized by plants. A relatively small, but quantitatively significant, number of terpenoids are involved in primary plant metabolism. However, the vast majorities are classified as secondary metabolites; compounds not required for plant growth and development but presumed to have an ecological function in communication or defense and are widely used in industrial applications. Terpene hydrocarbon scaffolds are generated by the action of the mechanistically intriguing family of mono-, sesqui-, and diterpene synthases collectively termed as terpene synthases, that catalyze multistep reactions with diphosphorylated...
Tipo: Poster
Palavras-chave: Biotechnology; Molecular Cell Biology; Bioinformatics; Plant Biology.
Ano: 2012
URL: http://precedings.nature.com/documents/6909/version/1

Welfare Impacts of Cross-Country Spillovers in Agricultural Research
Provedor de dados: 31
Autores: Lence,   Sergio H.; Hayes,   Dermot J.
The welfare implications of intellectual property protection (IPP) for private sector agricultural research are analyzed, focusing on the realistic cases in which countries provide different IPP levels, technology spills over across countries, and the public sector is involved in research. A model is developed to determine who benefits from, and who should pay for, the associated research. The paper contains some interesting results on the implications of a harmonization of IPP policies through multilateral agreements or via technology that allows research firms to prevent the copying of plants and animals that express traits that have emerged from their research.
Tipo: Working or Discussion Paper
Palavras-chave: Biotechnology; GURTs; Intellectual property; Research spillover; Welfare analysis; Research and Development/Tech Change/Emerging Technologies.
Ano: 2007
URL: http://purl.umn.edu/9375

Where Is Scientific Evidence in Support of Refuge Size Reduction for Pyramided Bt Crops?
Provedor de dados: 17
Autores: Andrei Alyokhin.
Genetically modified plants expressing insecticidal Cry proteins originating from a soil-dwelling bacterium Bacillus thuringiensis (Bt) provide a powerful tool for managing insect pests. Unfortunately, insect ability to develop resistance to insecticidal proteins potentially jeopardizes its long-term efficiency. Review of registration materials submitted by plant biotech industry to the U.S. Environmental Protection Agency and the existing scientific literature shows that currently available evidence in support of reducing refuge for the pyramided genetically modified corn plants to 5% of the total crop area is essentially limited to predictions of a single unpublished mathematical model developed &amp;#x201c;in-house&amp;#x201d; by the industry...
Tipo: Poster
Palavras-chave: Biotechnology.
Ano: 2011
URL: http://precedings.nature.com/documents/6563/version/1

Why not &amp;#x22;do simple things in a simple way&amp;#x22;: Use of the Pap test as the first step in screening genetic stability for human cultured stem cell therapy?
Provedor de dados: 17
Autores: Katherine Athayde Teixeira de Carvalho; Ana Carolina Irioda; Larissa Zocche; Carolina Maria Costa de Oliveira Souza; Reginaldo J. Ferreira; Eduardo Aliprandini; Ricardo C Cunha; Julio C Francisco; Luiz Cesar Guarita-Souza; Mariester Malvezzi; Miriam Beltrame; Lismary Mesquita; Diogo Kuczera; Rafael Vargas.
The aim of this study was to analyze adipose tissue-derived mesenchymal stem cells (AT-MSCs) using the Pap test as a first screening step to evaluate genetic stability. Human adipose tissue from six healthy female donors was obtained from elective liposuction procedures. The cells were isolated, cultivated at P2/P3, characterized by flow cytometric analysis, and differentiation induced. The AT-MSCs were stained by Papanicolaou staining and analyzed according to the Bethesda classification, and viability-apoptosis relationships were evaluated. The results of the Pap test for Sample I indicated high-grade alterations consistent with genetic instability; for Samples II-V, atypical cells of undetermined significance; and for Sample VI, normal cells. These...
Tipo: Manuscript
Palavras-chave: Biotechnology; Molecular Cell Biology.
Ano: 2011
URL: http://precedings.nature.com/documents/5676/version/1

Without magic bullets: the biological basis for public health interventions against protein folding disorders
Provedor de dados: 17
Autores: Rodrick Wallace.
Protein folding disorders of aging like Alzheimer&amp;#x27;s and Parkinson&amp;#x27;s diseases currently present intractable medical challenges. &amp;#x27;Small molecule&amp;#x27; interventions - drug treatments - often have, at best, palliative impact, failing to alter disease course. The design of individual or population level interventions will likely require a deeper understanding of protein folding and its regulation than currently provided by contemporary &amp;#x27;physics&amp;#x27; or culture-bound medical magic bullet models. Here, a topological rate distortion analysis is applied to the problem of protein folding and regulation that is similar in spirit to Tlusty&amp;#x27;s (2010a) elegant exploration of the genetic code. The formalism produces...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4847/version/1

Without magic bullets: the biological basis for public health interventions against protein folding disorders
Provedor de dados: 17
Autores: Rodrick Wallace.
Protein folding disorders of aging like Alzheimer&amp;#x27;s and Parkinson&amp;#x27;s diseases currently present intractable medical challenges. &amp;#x27;Small molecule&amp;#x27; interventions - drug treatments - often have, at best, palliative impact, failing to alter disease course. The design of individual or population level interventions will likely require a deeper understanding of protein folding and its regulation than currently provided by contemporary &amp;#x27;physics&amp;#x27; or culture-bound medical magic bullet models. Here, a topological rate distortion analysis is applied to the problem of protein folding and regulation that is similar in spirit to Tlusty&amp;#x27;s (2010a) elegant exploration of the genetic code. The formalism produces...
Tipo: Manuscript
Palavras-chave: Biotechnology; Developmental Biology; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2010
URL: http://precedings.nature.com/documents/4847/version/2

Worm-like carbon shell chains produced from wood
Provedor de dados: 17
Autores: Kyoko Suzuki; Hidetoshi Kita; Yukie Saito; Tsutomu Suzuki; Kazuhisa Sato; Toyohiko Konno.
Large-scale utilization of wood which command absolute majority in biomass for functional carbon precursors contributes to reduce greenhouse effect. Wood char generally has a limit on material usage because of its non-graphitic structure^1^, so we developed a new functional wood char by iron-, or nickel-catalyzed carbonization, which has a graphite-like structure with mesopores good for electroconductivity and liquid phase adsorption capacity for macro molecules^2-5^. However the fine structure of the wood char is still not clear. Here we report more than 70 wt % of iron-catalyzed wood char is filled with chained carbon shells formed by 3~20 defective stacking layers of carbon hexagonal planes, which look like nanometer-sized worms swarm. We name them...
Tipo: Manuscript
Palavras-chave: Biotechnology; Chemistry.
Ano: 2011
URL: http://precedings.nature.com/documents/6519/version/1

X-ray phase contrast imaging of biological specimens with tabletop synchrotron radiation
Provedor de dados: 17
Autores: Stefan Kneip; Chris McGuffey; Franklin Dollar; Michael S. Bloom; Vladimir Chvykov; Galina Kalintchenko; Karl Krushelnick; Anatoly Maksimchuk; Stuart P. D. Mangles; Takeshi Matsuoka; Zulfikar Najmudin; Charlotte A. J. Palmer; Joerg Schreiber; Will Schumaker; Alexander G. R. Thomas; Victor Yanovsky.
Since their discovery in 1896, x-rays have had a profound impact on science, medicine and technology. Here we show that the x-rays from a novel tabletop source of bright coherent synchrotron radiation can be applied to phase contrast imaging of biological specimens, yielding superior image quality and avoiding the need for scarce or expensive conventional sources.
Tipo: Manuscript
Palavras-chave: Biotechnology; Cancer; Microbiology.
Ano: 2011
URL: http://precedings.nature.com/documents/5946/version/1

Yeast surface 2-hybrid to detect protein-protein interactions via the secretory pathway as a platform for antibody discovery
Provedor de dados: 17
Autores: Xuebo Hu; Sungkwon Kang; Xiaoyue Chen; Charles B. Shoemaker; Moonsoo M. Jin.
High throughput methods to measure protein-protein interactions will facilitate uncovering pairs of unknown interactions as well as designing new interactions. We have developed a platform to detect protein interactions on the surface of yeast, where one protein (bait) is covalently anchored to the cell wall and the other (prey) is expressed in secretory form. The prey is released either outside of the cells or remains on the cell surface by its binding to the bait. The strength of their interaction is measured by antibody binding to the epitope tag fused to the prey or direct readout of split fluorescence protein complementation. Our novel &amp;#x27;yeast surface 2-hybrid&amp;#x27; system was found to differentiate 6-log difference in binding affinities...
Tipo: Manuscript
Palavras-chave: Biotechnology.
Ano: 2008
URL: http://precedings.nature.com/documents/2067/version/1