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Ovocalyxin-32, a novel chicken eggshell matrix protein Inra
Gautron, J.; Hincke, M.T.; Mann, K.; Panhéleux, M.; Bain, M.; McKee, M.D.; Solomon, S.E.; Nys, Y..
The eggshell is a highly ordered structure resulting from the deposition of calcium carbonate concomitantly with an organic matrix upon the eggshell membranes. Mineralization takes place in an acellular uterine fluid, which contains the ionic and matrix precursors of the eggshell. We have identified a novel 32-kDa protein, ovocalyxin-32, which is expressed at high levels in the uterine and isthmus regions of the oviduct, and concentrated in the eggshell. Sequencing of peptides derived from the purified protein allowed expressed sequence tag sequences to be identified that were assembled to yield a full-length composite sequence whose conceptual translation product contained the complete amino acid sequence of ovocalyxin-32. Data base searches revealed that...
Tipo: Journal Article Palavras-chave: OEUF; COQUILLE D'OEUF; RECHERCHE AVICOLE; OVOCALYXINE; SEQUENCE NUCLEOTIDIQUE; TECHNIQUE ANALYTIQUE; MICROSCOPIE ELECTRONIQUE; TRANSMISSION; EXPRESSION DES GENES.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300012761095478&uri=/notices/prodinra1/2010/10/
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Molecular cloning and ultrastructural localization of the core protein of an eggshell matrix proteoglycan, ovocleidin-116 Inra
Hincke, M.T.; Gautron, J.; Tsang, C.P.W.; McKee, M.D.; Nys, Y..
The role of avian eggshell matrix proteins in shell formation is poorly understood. This calcitic biomaterial forms in a uterine fluid where the protein composition varies during the initial, calcification, and terminal phases of eggshell deposition. A specific antibody was raised to a 116-kDa protein, which is most abundant in uterine fluid during active eggshell calcification. This antiserum was used to expression screen a bacteriophage cDNA library prepared using mRNA extracted from pooled uterine tissue harvested at the midpoint of eggshell calcification. Plasmids containing inserts of differing 5′-lengths were isolated with a maximum cDNA sequence of 2.4 kilobases. Northern blotting and reverse transcriptase-polymerase chain reaction demonstrated that...
Tipo: Journal Article Palavras-chave: RECHERCHE AVICOLE; OEUF; CLONAGE MOLECULAIRE; EXPRESSION DES GENES; TECHNIQUE ANALYTIQUE; TRANSCRIPTION; SEQUENCE NUCLEOTIDIQUE; COQUILLE D'OEUF; ULTRASTRUCTURE; STRUCTURE MINERALE; OVOCLEIDINE; PROTEOGLYCANE; MICROSCOPIE ELECTRONIQUE.
Ano: 1999 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300012762095479&uri=/notices/prodinra1/2010/10/
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Effects of separate calcium feeding on laying hens selected for low (R-) or high (R+) residual feed consumption Inra
Banga-Mboko, H.; Bordas, A.; Minvielle, F.; Leroy, P..
Tipo: Journal Article Palavras-chave: CONSOMMATION; POULE PONDEUSE; OEUF; POIDS CORPOREL; METHODE STATISTIQUE; COQUILLE D'OEUF.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300012928096384&uri=/notices/prodinra1/2009/11/
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