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Valeur énergétique de la chair de l'huître Crassostrea gigas estimée par mesures microcalorimétriques et par dosages biochimiques ArchiMer
Heral, Maurice; Deslous Paoli, Jean-marc.
The energetic values measured by the direct method and estimated by biochemical analysis of the annual cycle of ash-free dry weight of two Crassostrea gigas populations, show that the analytic results are inferior by 30% to those obtained by direct evaluation. The mean energetic amount is 4.84 cal.mg-1. Its seasonal variations, from 4.11 to 5.39 cal.mg-l, mainly depend on variations in the values of lipids. The mean conversion factor of lipids is 8.63 cal.mg-!, noticeably different from Brody's (9.45 caI.mg-I). This coefficient varies according to seasons from 7.85 to 9.38 cal.mg'-I. According to the biochemical evaluations, female oysters appear to he more energetic than male oysters when gonads are full. Direct evaluations, on the other hand, show no...
Tipo: Text Palavras-chave: Crassostrea gigas; Microcalorimétrie; Biochimie; Coefficient de conversion des lipide; S Bassin de Marennes-Oléron; Crassostrea gigas; Microcalorimetry; Biochemistry; Conversion factor of lipids; Bay of Marennes-Oleron.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/publication-2953.pdf
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Valeurs caloriques de la chair de l'huître Crassostrea gigas Thumberg : estimation directe et biochimique ArchiMer
Deslous-paoli, Jean-marc; Heral, Maurice.
Caloric values of the ash-free dried sofkt parts of Crassostrea gigas have been characterized, according to season using a microcalorimeter and biochemical composition. This study shows the difference between the two methods. A conversation factor (8,63 kcal-gr(-1) has been determined for lipid of pacific oyster. Thix work shows that reproduction implies a loss of energy (53% to 63%) for non juvenile oysters. The increase of energetic level necessary to growth is more important for juvenile oysters and is upper than what is lost for reproduction.
Tipo: Text Palavras-chave: Lipid; Energetic level; Crassostrea gigas; Oysters; Lipide; Teneur énergétique; Crassostrea gigas; Huîtres.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/acte-3162.pdf
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Valorisation des claires ostréicoles - Contrat de Plan IFREMER - Région Poitou-Charentes - Année 1994 ArchiMer
Soletchnik, Patrick; Goulletquer, Philippe; Razet, Daniel; Geairon, Philippe; Le Moine, Olivier; Faury, Nicole; Turpin, Vincent; Hamon, R.; Robert, Jean-michel; Hussenot, Jerome; Brossard, Nicolas.
Cette étude constitue une des trois actions engagées dans le cadre du Contrat de Plan IFREMER - Poitou-Charentes (1994-1998) sous l"intitulé Valorisation des Claires Ostréicoles. Les deux actions complémentaires concernent la compréhension et l'amélioration du verdissement des claires sous la direction du Pr. J.M. Robert de l'Université de Nantes/lSOMER et la production de masse de microalgues afin de stimuler la croissance de mollusques filtreurs sous la direction de Dr. J. Hussenot du CREMA L'Houmeau. Ce programme répond directement à la demande des professionnels afin d'optimiser les conditions d'élevage de l'huître creuse C. gigas en période d'affinage qui à l'heure actuelle, ne permettent pas le contrôle de la qualité finale du produit. L'objectif...
Tipo: Text Palavras-chave: Claires ostréicoles; Affinage; Capacité trophique; Paramètres; Marais expérimental; Culture diatomée; Huître creuse; Crassostrea gigas; Haslea ostrearia.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00044/15541/12918.pdf
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Variabilité et bioénergétique de la reproduction chez l'huître creuse Crassostrea gigas ArchiMer
Enriquez Diaz, Marta Rosalia.
The variability in reproductive cycle of Pacific oyster, Crassostrea gigas, was evaluated in situ (Bay of Veys, BOV and Marennes-Oléron bay, MOB) and in hatchery culture conditions. Two trophic levels were tested over one year: high food level (HFL) and low food level (LFL). We observed that the phytoplanktonic concentration (chlorophyll a) was the principal factor that influenced gametogenesis intensity and duration. The oysters reared within a high trophic ration (BOV and HFL) shown a hyper-gonad development. However, the spawning efficiency was reduced and partial. Therefore, the resorption period was prolonged until winter. Meanwhile, the oysters reared in a low trophic ration (MOB and LFL) produced a normal gonad with a total spawning in summer and...
Tipo: Text Palavras-chave: Aquaculture; Crassostrea gigas; Genetic factor; Ecophysiology; Gametogenesis; Aquaculture; Crassostrea gigas; Bilan énergétique; Ecophysiologie; Gamétogénèse.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/these-1095.pdf
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Variabilité génétique des stocks d'huîtres dans les Pertuis charentais ArchiMer
Cornette, Florence; Lapegue, Sylvie.
Afin de fiabiliser l'approvisionnement en juvéniles de bivalves dans les Pertuis charentais, une information importante à prendre en compte est l'origine des naissains captés. Ainsi, afin de tenter de déterminer la provenance du naissain capté dans chaque zone des Pertuis charentais, l'objectif de l'action Vargenet « Variabilité génétique des stocks d'huîtres dans les Pertuis charentais» est de déterminer si l'outil génétique peut être envisagé en complément des données de suivi de stocks et de modélisation. Pour cela, il est nécessaire de savoir si les stocks sauvages présents dans ces zones peuvent être différenciés génétiquement. Si c'est le cas, les cohortes de naissain captées pourraient alors être assignées à un stock parental, ce qui permettrait...
Tipo: Text Palavras-chave: Génétique; Variabilité génétique; Naissain; Stocks sauvages; Huître creuse; Crassostrea gigas; Pertuis Charentais; Charente-Maritime.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00055/16661/14154.pdf
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Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species ArchiMer
Huvet, Arnaud; Boudry, Pierre; Ohresser, M; Delsert, Claude; Bonhomme, F.
Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Cloning System, CA). White colonies (11,500) were screened by hybridisation with double-stranded DNA probes containing dinucleotide repeats. Poly(dAdC), poly(dAdG), poly(dAdT) (Pharmacia Biotech, Sweden) were mixed at equimolar ratio and radiolabelled by [-32P] alpha dATP and dCTP using a random priming labelling kit (Life Technologies, Germany). Filter...
Tipo: Text Palavras-chave: Crassostrea gigas; Oyster; Microsatellites.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-703.pdf
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Variations des caractères biométriques et des constitutants biochimiques d'une population C. gigas (Thunberg) d'un parc d'élevage du bassin de Marennes - Oléron en 1974 ArchiMer
Gras, Marie-paule; Gras, Paul.
At the beginning of its introduction, following the massive mortality rate of 1970-1971 that struck the Portuguese oyster, Crassostrea angulata (COMPS and GRAS 1973), the oyster from the Pacific, Crassostrea gigas, displayed excellent growth in addition to exceptional hardiness and resistance to mortality. But, since 1973, in most of the farming areas, there has been a slowdown in the growth of the oysters and an at times quite pronounced decrease in their quality. It was in order to better understand their adaptation to the environment and the causes of the lowering of their growth rate and their condition that a study was undertaken in 1974, in one of the largest areas of flat cultivation of the Marennes-Oléron basin, located on the east coast of the...
Tipo: Text Palavras-chave: Marennes Oléron; Biochimie; Biométrie; Huîtres; Crassostrea gigas.
Ano: 1975 URL: http://archimer.ifremer.fr/doc/1975/acte-2988.pdf
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Variations des productions de biodépôts (feces et pesudofeces) de l'huître Crassostrea gigas dans un estuaire macrotidal : baie de Marennes-Oléron ArchiMer
Razet, Daniel; Heral, Maurice; Prou, Jean; Legrand, Jacqueline; Sornin, Jean-marc.
The continuous obtention of current (speed and direction) turbidity and phytoplanktonic fluorescence was achieved for 9 successive days during the month of May 1988. Each two hours, during the day, the faeces and pseudofaeces productions of 7 oysters have been sampled individualy. The filtration rate were calculated in continuous. It appeared that the concentration in particular matter and also the planktonic fluorescence fluctuated in direct relation with the water depth, the origin of the water masses and the speed of the wind. During the tidal cycle the mean filtration rate was constant which induced a food consumption largely variable. The oysters were always above the limit of pseudofaeces production which regulated the ingested quantity. Their...
Tipo: Text Palavras-chave: Faeces; Pseudofaeces; Sort; Crassostrea gigas; Fèces; Psueofèces; Tri; Crassotrea gigas.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-3031.pdf
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Variations saisonnières in situ de la production et de la composition des biodépots de trois mollusques estuariens (Mytilus edulis, Crassostrea gigas, Crepidula fornicata) ArchiMer
Deslous-paoli, Jean-marc; Sornin, Jean-marc; Heral, Maurice.
The biodeposition was quantified on the field with sediment traps. All over the year, the biodeposition was the lowest for Crepidula fornicata. Mytilus edulis rejected more biodeposits during winter, and Crassostrea gigas during spring and autumn. The percentage of organic matter sonsumed and digested was higher during summer. The constituttive elements of the digested organic matter was not always the same, all over the year. The energy content of the digest fraction was higher in spring. For Mytilus edulis the ration intake was higher in winter and spring, related with the gametogenetic ans spawning period. An analysis of the mineral constutents of the natural food was made.
Tipo: Text Palavras-chave: Crepidula fornicata; Crassostrea gigas; Mytilus edulis; Digestibility; Biodeposition; Crepidula fornicata; Crassostra gigas; Mytilus edulis; Digestibilité; Biodéposition.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/publication-2691.pdf
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Variations spatio-temporelles de l'infestation des huitres creuses, Crassostrea Gigas, par les vers du genre Polydora (Annelides Polychetes), dans le cadre du reseau Ifremer/REMORA. ArchiMer
Duault, Céline; Gillet, Patrick.
The worms Polydora. damage the oysters, Crassostrea gigas, by foraging the shell, training the formation of galleries and chambers with mud. The IFREMER'S REMORA network which has in charge to survey the oysters Crassostrea gigas in France, has showed an increase of the infestation of oysters since 1993 (first year of the network). The study had for goal first to estimate the spatial and temporal variations of the infestation of the oysters in 1999, by using two methods: the infestation index, the number of worms by individual and a quality index, the Polydora Index (IP varies from 0 to 1) which is based on 5 classes of the oysters quality (from 0 to 4) in relation to the aspect of the valves
Tipo: Text Palavras-chave: REMORA network; Infestation; Crassostrea gigas; Polydora sp.; Réseau REMORA; Infestation; Crassostrea gigas; Polydora sp..
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-2447.pdf
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Vibrio aestuarianus zinc metalloprotease causes lethality in the Pacific oyster Crassostrea gigas and impairs the host cellular immune defenses ArchiMer
Labreuche, Yannick; Le Roux, Frederique; Henry, Joel; Zatylny, Celine; Huvet, Arnaud; Lambert, Christophe; Soudant, Philippe; Mazel, Didier; Nicolas, Jean-louis.
Extracellular products (ECPs) of the pathogenic Vibrio aestuarianus 01/32 were previously reported to display lethality in Crassostrea gigas oysters and to cause morphological changes and immunosuppression in oyster hemocytes. To identify the source of this toxicity, biochemical and genetic approaches were developed. ECP protease activity and lethality were shown to be significantly reduced following incubation with metal chelators, suggesting the involvement of a zinc metalloprotease. An open reading frame of 1836 bp encoding a 611-aa metalloprotease (designated yam) was identified. The deduced protein sequence showed high homology to other Vibrio metalloproteases reported to be involved in pathogenicity. To further confirm the role of this enzyme in ECP...
Tipo: Text Palavras-chave: Vibrio aestuarianus; Metalloprotease; Crassostrea gigas; Oyster; Hemocytes; Extracellular products.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12649/9588.pdf
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Vibrio gigantis sp nov., isolated from the haemolymph of cultured oysters (Crassostrea gigas) ArchiMer
Le Roux, Frederique; Goubet, A; Thompson, F; Faury, Nicole; Gay, Melanie; Swings, J; Saulnier, Denis.
Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B (gyrB), RNA polymerase Quo factor (rpoD), replication origin-binding protein (rctB) and transmembrane regulatory protein (toxR) genes, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that...
Tipo: Text Palavras-chave: Vibrio gigantis; Haemoplymph; Disease; Crassostrea gigas; Oyster culture.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-792.pdf
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Vibrio tubiashii en France: description d'isolats pathogènes affectant des mollusques et étude de leurs mécanismes de virulence ArchiMer
Mersni Achour, Rachida.
The oyster farming constitutes one of the major components of the global aquaculture. However, this sector is facing abnormal mortalities outbreaks that affect diploid and triploid oyster at their different life stages, in the hatcheries and in the field. During bivalve molluscs mortality events in France, bacteria initially classified into Harveyi group, were regularly isolated along with herpes virus, V. splendidus or V. aestuarianus. In order to fine tune the taxonomic affiliation of those isolates, a genotypic and phenotypic approach was used. The bacterial isolates, initially misclassified into the Harveyi clade, were shown to be genetically closed to V. tubiashii strains already recognized as the main causative agents of larvae and juvenile mollusc...
Tipo: Text Palavras-chave: Crassostrea gigas; Isolats français; Vibrio tubiashii; Hémocytes; Pathogénicité; Infection expérimentale; Produits extracellulaires; Facteurs de virulence; Metalloprotéases; Crassostrea gigas; French isolates; Vibrio tubiashii; Hemocytes; Pathogenicity; Experimental infection; Extracellular products.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00230/34093/32540.pdf
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Viral contamination and depuration of oysters under field conditions ArchiMer
Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L.
Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine...
Tipo: Text Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf
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Viral gametocytic hypertrophy of Crassostrea gigas in France: from occasional records to disease emergence? ArchiMer
Garcia, Celine; Robert, Maeva; Arzul, Isabelle; Chollet, Bruno; Joly, Jean-pierre; Miossec, Laurence; Comtet, Thierry; Berthe, Franck.
Viral gametocytic hypertrophy was reported for the first time in 2001 in Pacific oyster Crassostrea gigas in France. Since this date, the number of reported cases and the distribution area have increased every year; however, the cases are not associated with macroscopic signs or increased mortality rates. Both male and female gametes were hypertrophied and basophilic inclusions were observed in gamete nuclei, Transmission electron microscopy revealed the presence of viral particles in these intranuclear basophilic inclusions. These particles had characteristics similar to those of the Papillomaviridae and Polyomaviridae families: they were small, non-enveloped, icosahedral, and 44 to 56 nm in diameter, The viral particles were found in male, female and...
Tipo: Text Palavras-chave: Polyomaviridae; Papillomaviridae; Gonad; Viral garnetocytic hypertrophy; Pacific oyster; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-3288.pdf
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Virose de l'huître portugaise ArchiMer
Comps, Michel.
Tipo: Text Palavras-chave: Virose; Crassostrea angulata; Crassostrea gigas; Etiologie; Prophylaxie et traitement; Histopathologie; Virosis; Crassostrea angulata; Crassostrea gigas; Etiology; Control; Histopathology.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/00140/25076/23182.pdf
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δ15N of organic matter sources and benthic invertebrates along an estuarine gradient in Marennes-Oleron bay (France): implications for the study of trophic structure ArchiMer
Riera, Pascal.
This study examined the delta(15)N of suspended and sedimented particulate organic matter, primary sources of organic matter and benthic invertebrates along an estuarine gradient in Marennes-Oleron Bay, France. Particular emphasis was given to the use of delta(15)N as a tracer of the origin of organic matter and as a means of determining the benthic food web structure in estuarine environments. delta(15)N values indicated that there was direct utilisation of benthic diatoms as a food source by oyster Crassostrea gigas near intertidal mudflats but suggested trophic mediation between terrestrial detritus and oysters in the upper estuarine reaches. delta(15)N data showed that the trophic position of oysters may vary in the estuarine bay. The delta(15)N values...
Tipo: Text Palavras-chave: Nitrogen isotope ratio; Particulate organic matter; Crassostrea gigas; Benthic food web; Estuary; Marennes-Oleron Bay.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00448/55926/57786.pdf
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Итоги изучения фауны симбионтов промысловых черноморских двустворчатых моллюсков IBSS Repository
Холодковская, Е. В..
Для Mytilus galloprovincialis, Ostrea edulis и Crassostrea gigas у черноморских берегов России, Украины, Румынии и Болгарии приведен список видов симбионтов и их ареалы. Установлено, что характер распространения симбионтов определяется экологическим типом биоты. Приведены новые данные по морфометрии трематоды Parvatrema duboisi и инфузорий Raabella helensis и Crebricoma carinata
Tipo: Journal Contribution Palavras-chave: Симбионты; Паразиты; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas; Черное море; Parasites; Symbionts; The Black Sea Symbionts Parasites Symbionts Parasites http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_5574.
Ano: 2003 URL: http://repository.ibss.org.ua/dspace/handle/99011/488
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