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Only the B biotype of Bemisia tabaci is present on vegetables in São Paulo State, Brazil Scientia Agricola
Rocha,Kelly Cristina Gonçales; Marubayashi,Julio Massaharu; Navas-Castillo,Jesús; Yuki,Valdir Atsushi; Wilcken,Carlos Frederico; Pavan,Marcelo Agenor; Krause-Sakate,Renate.
Bemisia tabaci (Genn.) is one of the most important pests in cultivated areas of vegetables and ornamental crops around the world. Based on the mitochondrial cytochrome oxidize I (mtCOI) sequence, there is evidence that B. tabaci should be considered a cryptic species complex of 11 groups containing 24 species. Two of the groups, Middle East-Asia Minor 1 and Mediterranean include biotypes B and Q, respectively. In this study we evaluated the mtCOI sequence of B. tabaci populations collected in sites of the state of São Paulo, Brazil. Using PCR-RFLP with Taq I, a typical biotype B profile was obtained for all specimens. Based on the comparison with mtCOI reference sequences we found four haplotypes all belonging to the Middle East-Asia Minor 1. They...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RFLP; Cytochrome oxidase I gene; Sequencing; Whiteflies.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000100018
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Molecular detection of adulteration in commercial buffalo meat products by multiplex PCR assay Ciênc. Tecnol. Aliment.
WANG,Lanping; HANG,Xinru; GENG,Rongqing.
Abstract Authentication of commercial buffalo meat products has become a market concern. This study intended to develop and validate a highly species-specific multiplex PCR assay for authentication of buffalo meat products. Four pairs of species-specific primers were used to target mitochondrial cytochrome oxidase I (COI) gene. The assay generated the expected PCR products of 313, 255, 294 and 177 bp for buffalo meat, cattle meat, pork meat and duck meat, respectively. The multiplex PCR assay was sensitive enough to detect 1 pg pure DNA and 0.1% (w/w) adulterated meat under mixed matrices. Market survey revealed about 35.3% of buffalo meat products are adulterated with cattle meat, pork meat or duck meat in China. The adulteration was found in all food...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo meat; Commercial fraud; Adulteration; Multiplex PCR; Cytochrome oxidase I gene.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000200344
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